LOCUS       DASYWM010000001         4711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_656861, whole genome shotgun sequence.
ACCESSION   DASYWM010000001 DASYWM010000000
VERSION     DASYWM010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656861"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..472
                     /locus_tag="VGR29_00005"
     CDS             59..472
                     /locus_tag="VGR29_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2071998.1"
                     /translation="MRFRFDIVVYAFLGMAVAIAIAAVVSGSGEADATGSAAVPHYQR
                     ADVRVRCTDEPQISRGQDPMLVQEEYGTIPSTVSHLTILTDENCQPDAEGVSHCRNRV
                     QFETATGIEQATLRHHHRMAEEPCFTPGQYVEVVR"
     gene            complement(553..1344)
                     /locus_tag="VGR29_00010"
     CDS             complement(553..1344)
                     /locus_tag="VGR29_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2071999.1"
                     /translation="METNVGGDNPSSEKDAPPTIPLDRQHLLFDADDTLWENNVHFER
                     SVEEFVRFLDHEHLAPREIRAMLDEIELANVSTHGYGARAFAHSLSETFQQITGSNDE
                     QALESVRAFGLRILDIEMEPIEGVQETLEALRPHHDLFLITKGDPEEQRLKIDRSAAS
                     QIFDAHVITQEKRTNTYRDIVESLKLDPLRTWMIGNSTRSDIHPALEAGLHAVLIPHP
                     MTWHLEHVDIEHHPGWKGQFVELGSFRELLTLFRHSPSRSHGRMD"
     gene            complement(1516..1968)
                     /locus_tag="VGR29_00015"
     CDS             complement(1516..1968)
                     /locus_tag="VGR29_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV2072000.1"
                     /translation="MPLTGEYAPTTSDWARQQTELFETSGGKDGNTLRGMPIIVMTSV
                     GAQSGKLRKTPLMRVEHNGEYAIVASLGGAPRHPAWYFNVKKNPHVELQDGPVKRDYL
                     AREVTGEEKAVWWERAVEAYPDYAEYQERTSREIPVFVLTPMEDEQKA"
     gene            2276..3220
                     /locus_tag="VGR29_00020"
     CDS             2276..3220
                     /locus_tag="VGR29_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072001.1"
                     /translation="MLRSIGLVLMVCALLIGNVAASAAQEIDISFSDVKLKELGYPEI
                     HITVGPDGVEAPSEIEAGYYLVTLHPTEEYSAYLDFMKPPAGLSEDEATELALQAAAN
                     DLVQPDWEYLGGTNTFEIGVPVSFAIHFQPGEYVTAASYYAPEEGAEEVMTLVPLVVT
                     GEATPAAMVATPVVAQASPVASAPMADVTLEMTDDLEYIVSPDPVPSGPQLWEVTNTG
                     TEQAHHLVMQRVPEGVTAEEIVAEFAGLISGTPPAGEPLVTQFTYVGYAALQSGGHTT
                     WQEFDLDPGTYAITCFIIDPATGVPHLLDGMVTTFTVE"
     gene            3416..3652
                     /locus_tag="VGR29_00025"
     CDS             3416..3652
                     /locus_tag="VGR29_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072002.1"
                     /translation="MPLRKREDIQKRERTTIEEAREQGIGEPGDGVEPTVPTYTDSGG
                     VSDLGVDEPVVPGQLRGDPSPEPEGDVDVAGRGS"
     gene            3943..>4711
                     /locus_tag="VGR29_00030"
     CDS             3943..>4711
                     /locus_tag="VGR29_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2072003.1"
                     /translation="MDAGSYRFMLGEFECLAVSDGAFNYSPGSLFANVPEQEIESVLH
                     SRGLPTDRVTTPYTCLFIDTGDNRVLVDTGAGNLGESAPKVVPNVDHSTTATGALRRN
                     LEAAGIPASEVDTVIITHAHPDHVGGTVDDAGELVFANARYVIGREESTFWTSEVGAR
                     QAPPIMVDIARRSLDAIGDRLTFVDTGEEILPGIRVVATPGHTPGHISLEISSGDEIL
                     LHISDVVIYPLHLEHPDWTPVFDMLPEAAETSKRRTFD"
BASE COUNT          890 a         1329 c         1486 g         1006 t
ORIGIN      
        1 gtcaggtcgt gtgccggtgg ccaatccgtc ggcctgagcg agggcaggga gttatccaat
       61 gaggtttcgg ttcgatattg tcgtctatgc gttccttggt atggcggtcg caatcgctat
      121 agcagcagtc gtctcaggga gcggagaggc agacgccacc gggagtgctg ctgtgcccca
      181 ttatcaacgg gctgatgtgc gcgttcggtg taccgatgag ccacagatct cgcgaggcca
      241 ggacccgatg ctggtgcagg aggagtacgg cacaatcccc agcaccgtgt cacatttgac
      301 gatcctgact gacgagaact gtcagccgga cgcggagggc gtctctcatt gccgcaaccg
      361 tgtgcagttt gagacggcga cggggatcga gcaggcaacg cttcggcatc atcatcgtat
      421 ggcggaggag ccctgcttca cgccgggtca atacgtggaa gtggttcgat agggtcagtg
      481 ggtgaaacca catctgcagg gcatgcatcg ggaagcgtgt gcggacgtat cgctgacgca
      541 agattggccg tatcagtcca tccttccatg gctcctgctg gggctgtgac ggaaaagcgt
      601 gagcagctca cggaagcttc ccagctccac gaactgacct ttccatccgg gatggtgttc
      661 gatatccaca tgctccaggt gccaggtcat gggatggggt atcaggacgg cgtgcaggcc
      721 tgcctcaagg gccgggtgga tgtcggagcg cgtggagttc ccgatcatcc aggttcgcaa
      781 ggggtcaagc ttgagtgact cgacaatgtc ccggtaggtg ttggtgcgtt tctcctgtgt
      841 gatgacgtga gcatcgaaga tctggctggc cgcagaccgg tcgatcttca ggcgttgctc
      901 ctccgggtct cctttggtga tcaggaagag atcgtgatgg gggcgaagtg cctccagcgt
      961 ctcctgaacg ccctcgatgg gctccatctc gatgtcgagg atgcggagac cgaacgcgcg
     1021 gacgctttcc agcgcttgct cgtcgttgga acccgtgatc tgctggaagg tctcggaaag
     1081 actgtgggcg aacgcccgcg caccgtagcc gtgggtggac acgttggcca gctcgatctc
     1141 gtcgagcatt gcgcgtattt ctcggggggc gaggtgctcg tgatcgagga agcgcacgaa
     1201 ctcctcgacc gagcgctcga agtgaacgtt gttctcccag agcgtgtcgt cggcatcaaa
     1261 gagcagatgc tgccgatcca gcgggatagt gggcggggcg tccttctcac ttgatggatt
     1321 gtcaccgccg acgttggttt ccacgtttcc ctaccgctat gtgcttattc gacctgctgt
     1381 gccgacgtgg tacgaatgtg tcgatccggc agttcggttg gtcctggcac gctgtcaaag
     1441 acgagaccgt gcgcgaatcg caaacgagca cgggcggtat cggtccatcg ctcgatctgg
     1501 ttatgagacg tggagtcagg ctttctgttc atcctccatg ggtgtgagga caaagacggg
     1561 aatctcgcga ctcgtcctct cctgatactc cgcgtagtcg ggataagctt cgaccgcgcg
     1621 ttcccaccat acggcctttt cctcgcctgt gacttcgcgg gcgagatagt cccttttgac
     1681 gggtccgtcc tgcagctcga catgtgggtt cttcttcaca ttgaagtacc acgctgggtg
     1741 cctcggggcg ccaccgagcg atgcgactat cgcgtattcg ccgttgtgct caacgcgcat
     1801 gagtggagtc ttccgaagct tcccgctctg ggctccgacg gaggtcatta cgataatggg
     1861 catgccgcgc agcgtgtttc cgtccttacc ccctgatgtt tcaaagagct cggtttgctg
     1921 gcgggcccag tcggacgtgg ttggcgcgta ttctccagtg agtggcatgt gcggacggcc
     1981 ccatgtgttc aagcggacgg cgctccccat ttgggagcaa cgaacgaagg attcgttttt
     2041 acattgtgtg acgatgttcc ggcgaatgcc aatccggatg tttccagagg gcatggttcc
     2101 tgacccggca aggagcctgg ggacaggaaa caggcccggg atgccggtca gagagtcgct
     2161 gcatgacgga cctttcagtt ttccctgacc cttgcgtttt gatttcgctg tccctagact
     2221 ttttgtcggg acagattcac ttttgcgaag cgtatctgaa gaagggaaaa gaaccgtgtt
     2281 gcgatcaatc ggactcgtat tgatggtctg tgcgctgctc atcggcaacg tggcagcgtc
     2341 ggccgcacag gaaatagaca tctcgttcag cgatgtgaag ctgaaggagc ttgggtatcc
     2401 agagatccat atcacggttg gacctgacgg cgttgaagct ccgtccgaga tcgaggcggg
     2461 atattacctc gtcacgctcc acccgacgga ggaatattcc gcttacctgg acttcatgaa
     2521 gccgcctgcc gggttgtctg aagacgaggc gacggagctc gcgctccagg ctgcggccaa
     2581 tgatcttgtc cagcctgact gggagtatct cggcggcacg aacacattcg agatcggtgt
     2641 tcctgtatcg ttcgccatcc atttccagcc cggtgagtat gtaaccgccg cctcgtacta
     2701 cgccccggag gagggcgctg aggaggtcat gaccctggtg ccgttggtcg tcacgggcga
     2761 agccactccg gccgcaatgg tcgccacacc ggtcgtagcg caagcatcgc ctgtagccag
     2821 cgctccaatg gccgacgtga ccctggagat gacggacgat ctggagtaca tcgtctctcc
     2881 agacccggta ccaagtggtc cccagctttg ggaagtgacg aatacaggga ccgagcaggc
     2941 ccatcacctg gttatgcaga gagtcccgga gggggtaacg gcagaagaga tcgtggccga
     3001 gttcgcgggc ctgatctccg ggacgccgcc tgccggtgag ccgctcgtca cccagttcac
     3061 gtacgttggc tatgccgcgc tgcaatccgg tggccacacg acctggcagg agtttgacct
     3121 cgacccgggg acctacgcca tcacctgctt tatcatcgat cctgccactg gcgtgccgca
     3181 tctcctggat ggaatggtga cgaccttcac cgtggaatag aagcccgcgg ctgagcggcg
     3241 gtgacactga cattcactcc gggaatcgtc aattcttgtc cctgtctgcc tgcacgctgt
     3301 gcggccgacg gggacatttt aattaagtga cgtccgagag catcgtggag attgctggac
     3361 tactccaggc agggttccgg gtgggatact ggccgcacgt tgaagggaga agatcgtgcc
     3421 actgagaaaa cgggaagata tccagaagcg ggaacgcacg acaatcgaag aagcgcgcga
     3481 gcaggggatt ggcgagccgg gagacggagt ggagccaacg gtgccgacgt acacggactc
     3541 cggtggtgtt tcggatcttg gcgtcgatga accagtcgta ccggggcaat tgcgcggaga
     3601 cccgtcgccg gaaccagagg gtgacgtgga cgtagcggga cgagggtcgt aagacctcag
     3661 cttttcgccc gcgtggctcc ctgctcaagg tcgtgattcc cgctcagagc cctgtttcac
     3721 gtggcataac aggataacct gtcactgcaa ttggctttct cccagagcgt tcagttccat
     3781 caccggtcgt ctccggttgt ggaagcgatg catgatccgt atgatgcaga tgggacccat
     3841 gtttcatgtt ccgatcaatg tcgtgctgct gcgctctggt gaatcgggaa tgtggcgtgt
     3901 cgccgtttca tgttggtcat ggcgcgagag gagagcaggt atatggatgc cggatcctac
     3961 cgtttcatgc tcggggagtt tgaatgcctt gccgtcagcg atggcgcctt caactattcc
     4021 cccgggagcc tgtttgccaa cgtccctgag caggaaatcg agagcgtcct ccattcgcgg
     4081 ggactcccca ctgaccgggt cacgacgccc tacacctgtc tgttcataga cactggtgac
     4141 aaccgggtac tcgttgatac gggtgccgga aacctcggtg aaagcgcacc aaaagtggtc
     4201 ccgaatgtcg accactcgac gaccgcgacg ggtgccttgc gccgcaatct ggaggcggca
     4261 ggtatcccgg cgtccgaggt cgataccgtc atcatcactc atgcccatcc cgaccatgtc
     4321 ggaggcacag tggacgatgc aggggagctc gtctttgcca acgcccggta cgtcatcgga
     4381 agagaagagt cgactttttg gacgtcagag gtcggtgccc ggcaggctcc gcccatcatg
     4441 gtcgatattg ccaggcgcag cctggacgca atcggagatc ggctcacatt cgtcgatacc
     4501 ggtgaggaga tcctccccgg tatccgggtc gttgccaccc ccgggcacac cccgggacac
     4561 atatcgctgg agatctcatc gggcgatgag atcctgctgc acatctccga tgtcgtgatc
     4621 tacccgctgc acctcgagca tccggattgg accccggtct tcgacatgct ccccgaggcg
     4681 gctgagacga gcaagcgccg cacgttcgat c
//
LOCUS       DASYWM010000002         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_206592, whole genome shotgun sequence.
ACCESSION   DASYWM010000002 DASYWM010000000
VERSION     DASYWM010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206592"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VGR29_00035"
     CDS             <1..910
                     /locus_tag="VGR29_00035"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEV2072004.1"
                     /translation="GTLVKRYWYHFTSLIAIIVLMIIGFTPLRAVVYATGLAFLLSFL
                     RRDTAMTPNRTWNALRSGAVSTLSVASTCATAGIIVGVMTLTGLGLKMSDLIVDLANE
                     QLFLTVLYTGLAVWVLGLAVPVTASYIIAAAITAPALISMGVPDVAVHMFIFYYAVLS
                     EVSPPTALSPFAAAAITGGNPFKTMMLTWKYTLPAFVVPFMFTLNKENGINLLALGET
                     SDIILSTVTACLAILALVAGVGGWLLKPATWLERGILVAAAGLLLYTGPVQDVTGTAL
                     LVVGIALHVMRTRGSNEAGPTPAPAV"
     gene            complement(992..1933)
                     /locus_tag="VGR29_00040"
     CDS             complement(992..1933)
                     /locus_tag="VGR29_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2072005.1"
                     /translation="MVENPVLGTGQQRYSRILVPLDGSDASEAALAYAAQIPCRELVL
                     LHVVVDDDVIAPEWATGWEDDDEEEASARGTMEQLAERLRNGEREVSIDIRSGDIAEQ
                     VIEAGTDADLIVMKTHGRGAAGRLIFGSVADHVVRHGQTPTLLLRIGELTRSPREPKR
                     VAVALDGSELAERSLPVAARLAGALGLPMVLMRAVSVDQIKHAVAARRDPGKPPHEQP
                     PTLYEDTQRDVMAETAEYLEGHADRLRQSGLRVETQVLEGTAAFSLMWALTPEDVIVV
                     ATHGRGGYKRLSIGSVAEKLVREAPCPVLLQRATGGQ"
     gene            2455..2928
                     /locus_tag="VGR29_00045"
     CDS             2455..2928
                     /locus_tag="VGR29_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072006.1"
                     /translation="MKSKLNLIEETRQLRLKDFGVDKKTWSQLLEAFKHPVGAPGMFY
                     LAGRPFQMAFYLGFARRLMAEGVDPFNASQCKALADEVKQQVKQETGSQPGLGRNILL
                     HLLFPNEFERIASDDSKKRIVDHFAQDASVPSGTDVDNTLLIIRNHLAKKWIAPI"
     gene            2976..>3265
                     /locus_tag="VGR29_00050"
     CDS             2976..>3265
                     /locus_tag="VGR29_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2072007.1"
                     /translation="MNRGDDDSNGDETPESQKLDELMEATYVSKSFLQELNDILLDKK
                     QLIFEGPQGSGKTYVAENFARWFTGQSLDVNTPPNEQVEIVQFHQSYGYEDFV"
BASE COUNT          648 a         1018 c          927 g          672 t
ORIGIN      
        1 cgggacgctg gtgaagcgtt actggtatca cttcacctcg ctcatcgcga tcatcgtcct
       61 gatgatcatc gggttcacac cgctacgggc ggtggtctac gccacaggtc tggccttcct
      121 gctcagcttc ctgcgccggg acacggcaat gacgccgaat cgaacctgga acgcgctgcg
      181 cagcggtgcg gtcagcaccc tgtcggttgc gtccacctgt gccaccgccg gcatcatcgt
      241 tggggtgatg acgttgaccg ggctcggcct gaagatgtcc gacctgatcg tcgatctcgc
      301 caacgagcag cttttcctga ccgtgctcta cactgggctg gcagtgtggg tgctcggcct
      361 ggcggtgccg gtgacggcgt cgtacatcat cgcggcggcc atcacggctc cggcgctgat
      421 ctcgatgggc gtcccggacg tcgcggtcca catgttcatc ttctactatg cggtgctctc
      481 ggaggtgtcg ccgccaaccg ctctctcccc cttcgcggcg gcggccatca ccggcggcaa
      541 cccgttcaag acgatgatgc tgacatggaa atacaccctg cccgccttcg tcgtaccgtt
      601 catgttcacc ctgaacaagg aaaacggaat caacctgctg gcgcttggtg agacatcgga
      661 catcatcctc tccacggtga ccgcctgcct cgcgatcctg gcactggtcg cgggcgtcgg
      721 cggatggttg ctcaaacctg ccacctggct ggaacgggga atcctcgtcg ccgccgcggg
      781 gctactgctc tacacgggtc cggtgcagga tgttaccggc accgccttgc tggttgtcgg
      841 cattgccctg catgtcatgc gcacacgggg cagtaacgag gcgggaccga cgcccgcccc
      901 ggcggtctag accctgttgt gtcgcgttgg gccccggcac aatatgctgc cggggcccaa
      961 cgtcatgaac aggtctacgg gtgattgggc gtcattgacc cccggtcgcg cgctgcagca
     1021 gcaccgggca cggggcctcg cgcaccagtt tctcggcgac actcccgatc gacaaccgct
     1081 tgtatccacc tcggccatgg gtggccacaa cgatgacgtc ctccggcgtt agcgcccaca
     1141 tcaggctgaa tgccgccgtg ccttcaagga cctgtgtctc gacccgcagg ccggattgcc
     1201 tcaatcgatc ggcatggccc tccagatact ctgccgtctc ggccatgacg tccctttggg
     1261 tgtcctcata gagcgttggc ggttgctcgt ggggaggctt gccagggtcg cgacgtgccg
     1321 ccacggcgtg cttgatctga tccacgctca ctgcccgcat caggaccatc ggcaacccga
     1381 gcgcacccgc cagcctggcg gctaccggca atgaccgctc ggccagctcc gatccatcca
     1441 gggcaaccgc cacccgcttc ggttcacggg gggatcgggt aagctcgccg atacgcagca
     1501 acagcgtcgg tgtctgcccg tggcgcacga catgatcggc aaccgatccg aagatgaggc
     1561 gaccggccgc gccacggcca tgcgtcttca tcacgatcag gtcagcatcc gtgccggcct
     1621 cgatcacctg ctccgcgatg tcacccgacc ggatgtcgat gcttacctcg cgctccccgt
     1681 tgcgcaggcg ttccgcgagc tgctccatgg tgccgcgggc ggatgcttcc tcctcgtcat
     1741 catcctccca gccggtggcc cactcgggag caatgacgtc gtcatcgacg acgacgtgca
     1801 gcagcaccag ctccctgcaa gggatttgcg cggcgtatgc cagcgcggct tcggaggcat
     1861 ctgagccgtc gagtggcacc agaatacggc tgtatcgctg ctgacccgtt cccaagaccg
     1921 ggttctcgac catggagtcc tccttgcctg gatggcgact caattcggat gatctgtcct
     1981 tatcaatgat accgccgacc gggactgccg cgatctagga aggcggttcc gatatctctc
     2041 cactactgcc attcgcgtgg gatgtgactc tatcggcgcg gtccggatcg aggtcggtgc
     2101 gccatggccc tctatcgttt cggaaagcac ccatatcatg acgatgtgaa aggacctatc
     2161 tctatggcgc gcgttcctgg cgcagaagcc gtctatgaag tcgccgatct cttccgctag
     2221 aggtgtctgg tcgagagcaa atctctgctc tggccggacc acaatgcatg gacacttgag
     2281 aatctcgata acttctgggc agcctttgtc gaccatccaa acgagagtac cgattcattc
     2341 gtcgtcaaac tgggcggaca gcttgaggct caatcggacg acattcaccg aattgccgtt
     2401 gatttggtca cagtttatgg gcccttccct agtagtggac caggaggcat ggggatgaag
     2461 tccaaactca atctaattga ggagacccga cagttgcggc tcaaagactt cggagtagat
     2521 aagaagacgt ggagtcaact gctggaagca tttaagcatc cagtaggtgc gccaggaatg
     2581 ttttatctcg ccggacgacc cttccaaatg gcgttctacc tcggattcgc caggcgattg
     2641 atggcagaag gtgtagatcc gtttaacgca tctcaatgca aggcactcgc tgatgaagtc
     2701 aagcagcagg tgaagcaaga gacaggatcc caaccgggtt tgggcagaaa catactgctt
     2761 cacctgcttt tccccaatga attcgaacgg atcgccagcg acgacagcaa gaagcgcatc
     2821 gtcgaccatt tcgctcaaga tgcgtctgta ccttctggta ccgatgtcga caatacgctt
     2881 ctcattatcc ggaaccactt ggctaaaaaa tggatcgccc caatttagat ttctacgagg
     2941 aaaacatcta ccagctatgg aacccgtcag aaattatgaa tcgcggtgat gacgacagta
     3001 acggcgatga aacgcccgaa tcacaaaaac tcgatgagtt gatggaagca acgtatgtta
     3061 gcaaatcctt cctccaagaa ctcaacgaca tactcctcga caagaagcaa ctcatcttcg
     3121 aaggacctca aggttcgggg aagacctacg tggccgagaa ttttgcgcgg tggttcaccg
     3181 gccaatcgct cgacgtcaac accccgccca acgagcaggt cgaaatcgtt caatttcacc
     3241 agtcatatgg gtatgaggac ttcgt
//
LOCUS       DASYWM010000003         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_150316, whole genome shotgun sequence.
ACCESSION   DASYWM010000003 DASYWM010000000
VERSION     DASYWM010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150316"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VGR29_00055"
     CDS             complement(<1..437)
                     /locus_tag="VGR29_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072008.1"
                     /translation="MAAHRRLVAVVSLMSLVVAIIGIVLISPNLSADGFVSEQAESAR
                     VDEALAREFGHGSDSLVFLFDANRLVSDPAVQRAVEGALAPITADARFGRVLTTWSTG
                     DPRMVSETGTATYAVAVVAPGADVDDAEFERFTGLVTDGAETNG"
     gene            complement(604..1305)
                     /locus_tag="VGR29_00060"
     CDS             complement(604..1305)
                     /locus_tag="VGR29_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2072009.1"
                     /translation="MLGKAVPSGASARRERGRREMRLAIIDTASRIIADEGIDRLTIR
                     AVATRLGYSPGALYEYFDSKEAILAGLYFEGTDGLGARCEQAVADLPVGASAIDALMA
                     LGHAYRVFALEHADLYRLVFGGFKTPPQPERAGYPDANQGGFGTLVQVAQRGVEEGVF
                     IDLPAPVIAVAAWSAVHGFVSLELSHHLTGGDGPGMPPTSPDEGRQRRDHMFDALVRM
                     VMLGFVREEHRPQSI"
     gene            complement(1699..>2539)
                     /locus_tag="VGR29_00065"
     CDS             complement(1699..>2539)
                     /locus_tag="VGR29_00065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2072010.1"
                     /translation="SFGIKTAPQHTTYDDMLKVWQEAEEIPSIEHAWLFDHFAPIQGD
                     VSGPCLEGWTVLAAFAAVTKRMRLGLMVTGNTYRHPAVLAKIAATMDVISDGRLDFGI
                     GAGWNVYEHESMGIPLYKPGERIRRLDEACEIIKGLFTQPTLDFDGRYYQLKDARSEP
                     KPVQKPYPPFVIGGSGEQLTLRVVAKHADIWNYTGPSVEEFTHKVRVLHDHCAVVGRD
                     PGEIVLSYQHRLQADDLESSVSELQRFVEAGATHIVLYLPTPHTDGIATRVAEEVIAK
                     VSA"
BASE COUNT          519 a          792 c          753 g          475 t
ORIGIN      
        1 ccattggttt cggcgccatc agtgaccagt ccggtgaagc gctcgaactc ggcatcgtcc
       61 acgtctgccc caggggcgac cacggcaacg gcataggtgg ccgtccccgt ctccgaaacc
      121 atgcgcggat ctccggttga ccacgtggtc agcacgcggc caaagcgtgc atctgcagtg
      181 atgggggcaa gggccccttc cactgcccgc tggaccgccg ggtcactgac cagacgattc
      241 gcatcgaaga gaaagacaag tgaatcgctg ccatggccga actccctggc gagcgcctcg
      301 tcgacgcggg cggattctgc ttgctctgag acaaatccat ccgcggagag gttgggtgag
      361 atcaggacaa taccaataat cgctacgacc agcgacatca gtgaaacaac ggcaacaaga
      421 cgtcggtggg ctgccaacag cagtcctcag gatgcaaaca tgacacactc cttccaagcc
      481 gggcacctat cctggttccc gggcatcatc gatagctaag agagttgaga tacagacttt
      541 acgacggtgg tcatggagcg gcgcaggatg ccggcctgca cggatgggcg tgtctgacga
      601 ccatcagatg gactgtgggc gatgctcctc ccgtacgaat cccaacatga ccatgcgcac
      661 gagcgcatcg aacatgtggt cccgacgctg ccgtccttcg tccggtgacg ttggcggcat
      721 cccggggcca tctcctccag tgagatggtg actcaactca agtgagacga acccgtgcac
      781 cgccgaccat gccgcaacgg cgatgactgg ggctggcagg tcgataaaca ctccttcctc
      841 aacgcctcgt tgcgcgacct ggaccagcgt gccgaaacct ccctgattcg cgtccgggta
      901 gccagctctt tccggctggg gtggtgtctt gaaccctcca aagaccaggc ggtagagatc
      961 cgcgtgttca agcgcgaaca cccggtaggc gtggcccagg gccatcagag catcgatcgc
     1021 cgaggctccc accggcaagt ccgcgacggc ctgttcacaa cgggcaccaa ggccgtcggt
     1081 tccctcgaag tagagaccgg caaggatcgc ttctttcgag tcaaagtatt cgtagagggc
     1141 accgggcgag tacccgaggc gagtggcaac ggcacggatc gtaagacggt cgatcccctc
     1201 gtcggcgatg atcctcgacg cggtgtcaat aatcgcgagt cgcatctcgc gccgaccgcg
     1261 ttcgcgcctg gcacttgctc cactaggaac cgctttacct aacaatggtc aatctcctga
     1321 acatcgttta gcaacttatg actccatcct atcatgaggt tcctgaatgt caataggcat
     1381 accgaacaat gttcagtgac ggaaggtcgg cgaacagtca cccgcccagg gaggcaagta
     1441 tgttcgtcct attcggcttg aagcaatgcc ggaagagggg acgaacatca tgctaccaag
     1501 attgattggt cgacatgcac agcgtcggct gttggaccgg gatcgagtag tcagggttac
     1561 atgcagctgg cgatgccgga ccaagccctg cagacggcgc tcctgcctgg gggccagttc
     1621 atctccatgc tgctccggca gccccggcga gggagcgcca aagctcccac cggacgcatc
     1681 gggagaggca gggcgcgctt atgcggagac tttggcaatt acctcctctg ccacccgcgt
     1741 tgcgattccg tccgtatggg gcgttggcag gtagagaaca atgtgcgttg cccctgcctc
     1801 aacgaagcgt tgcagctcgc taacggacga ctcgagatca tcggcctgga gccggtgctg
     1861 atacgagagg acaatctctc ccggatcccg acccaccact gcacagtggt catgcaggac
     1921 cctcaccttg tgcgtgaact cctcaactga gggtccggtg tagttccaga tgtccgcatg
     1981 cttggcgacc acccgcagcg ttaactgctc accactgccg ccgatcacaa agggcggata
     2041 cggcttctgc accggctttg gctcgctgcg cgcatccttc agctggtagt agcgaccgtc
     2101 gaaatcgagg gtcggctgcg taaacaagcc cttgatgatc tcgcaggctt cgtcgaggcg
     2161 gcgaatgcgc tccccgggct tgtacagcgg gatgcccata ctctcgtgct catagacatt
     2221 ccagccggca ccgatcccga agtcgagccg accatcggag atgacgtcca tcgttgcggc
     2281 gatcttggcc agcactgccg ggtggcgata ggtgttcccc gtcaccatca atcccaggcg
     2341 cattcgtttc gtgacggcgg cgaaggcggc aaggaccgtc catccctcaa ggcacggacc
     2401 actcacgtcc ccctgaatcg gggcgaagtg gtcaaagagc caggcgtgct cgatgctggg
     2461 aatctcctcg gcttcctgcc agaccttcag catgtcgtcg taggtcgtgt gctgcggcgc
     2521 ggtcttgatc ccgaagctc
//
LOCUS       DASYWM010000004         5893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_150320, whole genome shotgun sequence.
ACCESSION   DASYWM010000004 DASYWM010000000
VERSION     DASYWM010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150320"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..984)
                     /locus_tag="VGR29_00070"
     CDS             complement(112..984)
                     /locus_tag="VGR29_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2072011.1"
                     /translation="MATSLPTSSSLDPGNPPQPRSFRSAWLYGPALLWLLAAGLPFAF
                     VVLSSFKSVKEYIADLWAMPASLQLSNYLEAVEGGIGRFFINSLIVTVSSVVLTLVLA
                     SLASFPLAKMKFRLNRPLFLLFIAGAMIPVHVTLIPIYTLTNRLGIYDTLLALIGPYI
                     AFHLPLAVLIITEFMSHLPAELEEAARIDGAGPFEVFRRIILPLSTPALSTVAIYTFI
                     YAWNEFVFALVLLSSRSNMTLPLGLMQFSGEYSVNVPGIMAAVTLASLPIILFYLVMQ
                     ERVVAGMVAGAVKG"
     gene            complement(994..1947)
                     /locus_tag="VGR29_00075"
     CDS             complement(994..1947)
                     /locus_tag="VGR29_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2072012.1"
                     /translation="MARSDIQPRTLDTYAKPEPATLRGSSGFQFPQWLVAMLFIAPAL
                     LLYAIYVIYPIFQSILYSLRDWSGIGAGTYIGLDNYKEALSDGIFWRAVRNNIVLVIA
                     SIAIQLPIALMLALLLSAKFPGWRIFRAVFFLPLLLSTVAVGLVWINIYDPVFGLLNG
                     ILGAVGLENLQQSWLGNESTALPAIIAVVCWQYIPFYMILFIAGLTTIPLELYEAARI
                     DGASGWRSFWDITLPLLRGIIRTGAILSLIGSLKFFDLVWVMTGGGPNRSTELMATYM
                     YEEAFASFRMGYGSAVATLLFIFAFVIAGSTLIIDQRRSNR"
     gene            complement(2033..3379)
                     /locus_tag="VGR29_00080"
     CDS             complement(2033..3379)
                     /locus_tag="VGR29_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2072013.1"
                     /translation="MTVDKSRILRPSVTSSHLSRRRMLQGAAALGIAAPLARHTGIVG
                     AQEATPVGGDQEGTLTVWSLTGAGLNEELPQTAAARLTEAYPNVEMDLQALQNDPFKT
                     KLRTSLGSDNPPDVWHSWGGGVLGEYAKEGVVLDLTEALNQDGWKDRFIPGLLDIMKV
                     DDSYYGVPIVANTVVFWYNAELLGDISAPATWDEFIQLVKDVKSDGLVPITLANQTKW
                     PGAFYLNYLVLRINGPDFLDRVLAGEASFDDPGVVRAGEMIQELVDAGAFPEGFNGLD
                     IDTGGSRKLLYAEQAAMELMGTWLPGTIDSEIDDFSQNLSFFSFPQVEGGEGDPSSLL
                     GGVSPAYAVSQQTEIPEVAIQFLREITNDASRDLVVDAGRLAALKGATYDTETANQLA
                     TALEEAATVQLFWDQFFPPALGQAQLDVSQGLLSKSITPQEGAAQLEEAAASVREG"
     gene            complement(3537..4184)
                     /locus_tag="VGR29_00085"
     CDS             complement(3537..4184)
                     /locus_tag="VGR29_00085"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEV2072014.1"
                     /translation="MAKKALIFYGGWAGHEPEKTSARFAGILRGHGYDVDLEQTLDVF
                     TDADRTAQYDLIVPMYTQSEITKEQEKGLLDAVKAGAGLAGWHGGMGDAFRNATEYQF
                     ACGGQWVAHPGNIIDYTVNITNHDDPITAGLSDFQMHSEQYFLHVDPSNEVLVTTTFT
                     GEHAPWIAGTVMPVVWKRKYGEGKVFYCSLGHVDADFEVPEAQTLTERGLIWATR"
     gene            complement(4288..5400)
                     /locus_tag="VGR29_00090"
     CDS             complement(4288..5400)
                     /locus_tag="VGR29_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2072015.1"
                     /translation="MAERTNAVGIGIIGAGYISTKYLENCTGRFDNLKVVGIADVMME
                     RAEEQAQGFGIEALSVDQLLAHPDIELVINLTIPAAHGQVGLQVVNAGKSIYNEKPLA
                     ATREEAQAILARAAEQGVLVGSAPDTFLGSGFQTARALLDSGAIGRPVAVTGMLLLRG
                     HERWHPNPDFYYQPGGGPLFDMGPYYLAALMSLLGSVERVAATASASYQTRTIASGPR
                     AGETVPVETPTHVISVLDFANGVRCSLTTTFDVYDTNRSTLVLYGSEGTIRLPDPNTF
                     GGPVEMLRAGSDQWESVDLLEGYTDNSRGLGVSDMAQALRDGRAPRASGELGYHIVDV
                     MHAALESNDQGRHISIESNFERPVALGAVQVTGGQG"
BASE COUNT         1296 a         1681 c         1730 g         1186 t
ORIGIN      
        1 cccacccggc ctggccctca acgctagctg gaatgtatcg actccgtgcg cggcgattga
       61 tgccttgtga tagtgcatag gcacctcttg cctggaatac cgcgaaggca cttacccctt
      121 gacggcgcct gcgaccatcc cggcgaccac tcgttcctgc atgaccaggt agaagagaat
      181 gattgggaga ctggccaacg taacagcggc catgatcccg gggacgttga cgctgtactc
      241 gcccgagaac tgcatcagtc ccagtggaag cgtcatattc gagcgggagg acaggagtac
      301 cagcgcaaaa acaaattcgt tccaggcata gatgaacgta tagattgcca ccgtgctcag
      361 cgctggggtg ctcagtggca ggatgatgcg ccggaagacc tcaaacggtc ccgctccgtc
      421 gatcctggcc gcttcctcca gctccgcggg taggtggctc atgaactcgg tgatgatcag
      481 cacggccagc ggaagatgga aggcgatata gggtccaatc agcgcaagga gtgtgtcgta
      541 gatgccaagg cggttggtga gtgtgtagat cgggatcagg gtgacatgca ccgggatcat
      601 tgcgccggcg ataaagagga gaaaaagcgg ccggttcaag cggaatttca tcttggcaag
      661 agggaagctt gccagtgatg caagcacgag cgtaagcacc actgatgaaa cggtcacgat
      721 aagactgttg atgaagaacc tgccgatgcc cccctccacg gcctcgagat agttagagag
      781 ttgcagggat gccggcatcg cccagaggtc agcgatgtac tccttgaccg atttgaatga
      841 agagagcacc acaaaggcaa atggcagccc tgccgccagt agccacagga gtgccggccc
      901 gtagagccat gcagagcgaa acgaccgcgg ctgtggcggg ttgccggggt caagagagga
      961 ggaagtgggg agtgacgtgg ccatggacaa ctcctacctg ttcgatcggc gctggtcgat
     1021 gatgagggtg gatcctgcga tcacaaaggc gaagatgaag agcagagtcg ctacggcgct
     1081 cccgtagccc atccggaacg aggcgaacgc ctcctcgtac atataagtcg ccatcaactc
     1141 agtggacctg ttggggccgc cacccgtcat gacccacacg agatcgaaga atttcagcga
     1201 tccgatgagc gagagaatgg caccggtgcg gataatcccc cggagcaagg gcagggtgat
     1261 gtcccagaaa gagcgccatc ctgaagcccc gtcaattctg gctgcctcat acaactcgag
     1321 cggaatggtt gtgagcccgg cgataaagag aatcatgtag aaggggatgt attgccagca
     1381 cacgaccgca ataatcgcag ggagggcagt cgattcattt ccgagccatg actgttgaag
     1441 gttctcgagt ccaacggctc ccaggatacc gttcagcaaa ccaaaaacag gatcatagat
     1501 gttgatccag accagtccaa cggccaccgt cgaaagcagg agagggagga aaaacactgc
     1561 ccggaagatg cgccatccgg gaaatttcgc ggacagcaac aatgcaagca tgagggcgat
     1621 gggcaactgg atggcgatag aggcgatgac tagaacgata ttgttccgga ccgctcgcca
     1681 gaagatgccg tcgctcagcg cctccttgta gttgtccagc ccaatatacg tgccggcccc
     1741 gatcccggac cagtcgcgga ggctgtagag gatcgactgg aaaataggat agataacgta
     1801 gatggcgtac aggagaagag cgggtgcgat gaagagcatc gccacaagcc actggggaaa
     1861 ctggaacccg ctcgagccgc gtagcgtcgc tggctcgggc ttcgcatagg tgtccagtgt
     1921 ccgaggctga atatctgacc gtgccactcc cacacctcac gctaccggat caggattcat
     1981 gtgtgaacag ggctgtgcca ggaactcgtg acgcagccct gttcccggat tactagcctt
     2041 cgcgaactga ggcagccgct tcctcaagct gggcagcgcc ctcctgaggt gtgatggact
     2101 tgctcaacaa gccctgcgaa acatcaagct gggcctggcc gagggctggt gggaagaact
     2161 gatcccagaa gagctgaacc gtcgctgctt cttcgagcgc ggtcgcgagt tgatttgccg
     2221 tttcggtgtc ataggttgcg cccttcagcg ccgccaggcg cccagcgtcc accacaagat
     2281 cccgggaggc gtcgtttgta atctctcgca ggaactggat cgccacttcc gggatttccg
     2341 tttgctggga aaccgcatac gcagggctca caccgccgag aagggagcta gggtcaccct
     2401 cgccgccttc cacttgcggg aaggagaaga aggacaggtt ctgggagaag tcatcgatct
     2461 cagaatcaat ggtacccggc aaccaggttc ccatcagctc catcgcggcc tgctcggcat
     2521 agagcagctt gcgggatccg cccgtgtcga tatcgagacc gttgaacccc tcggggaagg
     2581 ctccagcatc gacgagttcc tggatcattt cgccggcccg gacgacgccc ggatcgtcga
     2641 aacttgcctc tccggccagg acccggtcca ggaaatcggg accgttaatc cgcagcacca
     2701 ggtagttcag gtagaaggct cccggccact tcgtctggtt cgcgagcgta atgggcacga
     2761 ggccgtccga cttgacgtcc ttcaccaact ggatgaactc atcccaagta gcgggtgccg
     2821 aaatgtcccc gaggagctcg gcgttgtacc agaagaccac tgtattggcg acgatcggga
     2881 caccgtaata ggagtcgtcc accttcatga tgtccagcag gccgggaatg aaacggtcct
     2941 tccatccatc ctggttcagc gcttctgtga ggtcgagaac cacaccttcc ttggcatatt
     3001 cccccaggac accaccgccc cacgaatgcc atacatcggg aggattatca ctaccgagcg
     3061 aggttcggag cttcgtcttg aacggatcgt tttgcagggc ctgaagatcc atctcgacgt
     3121 tcgggtaggc ctccgtcaac ctcgctgctg ccgtctgtgg aagctcctca ttcaaccccg
     3181 cgccagtgag actccaaaca gtcagcgtcc cttcctggtc gcctccaacc ggcgtggctt
     3241 cctgcgcacc aacgatgccg gtgtgtctgg ccaggggtgc cgctatgccg agtgcagctg
     3301 ctccctgaag catcctgcgg cgggaaagat gagacgaagt gacggatggg cgaagaatgc
     3361 gactcttgtc gacagtcatg cggttcctcc agatggatgt ttcaagtggc tggaaagtgc
     3421 cagcctctgc cagagcaatc cgatcggttg gcgaatttcg cgaatcttac tacagggttg
     3481 tccttacctg tcaacaggaa cccattattg ggaagtgcca ggaccagcga gttcgcctac
     3541 ctcgtagccc agattaggcc ccgctccgtc aacgtctgtg cctcaggtac ctcgaagtct
     3601 gcatcgacat ggccgagcga gcagtagaac accttcccct cgccatactt ccgcttccag
     3661 accactggca tcactgtgcc agcaatccag ggagcatgct ccccggtgaa tgtcgtcgtg
     3721 acgagcactt cgttcgacgg gtcgacgtgg agaaagtact gctcggaatg catctggaaa
     3781 tcactcaatc cggcggtaat ggggtcgtcg tgattggtga tattgacggt gtaatcgatg
     3841 atgttgccag ggtgggcgac ccactgtccg ccacaggcaa actggtattc ggtggcgttc
     3901 cggaatgcat cgcccatgcc gccgtgccag cctgctagtc ctgcaccggc cttgacggcg
     3961 tcgagcaatc ccttctcctg ttccttggtg atttcactct gggtgtacat gggaacgatg
     4021 agatcgtact gggccgtccg atccgcatca gtgaagacat ccaatgtctg ctcgagatcg
     4081 acatcatacc cgtgacctct caggattccc gcaaagcggg ctgaggtctt ttcgggctca
     4141 tgccctgccc agccgccata gaagatgagt gctttctttg ccatcgtgtg taactcctcc
     4201 gggtgatgtt cgccaccgct tgcttttgga ataccaccgc tcatctgaca cagcgtaaac
     4261 cgtcatagac gaatgtgtgg cgggatgtta gccctgccca ccggttacct gaacggcgcc
     4321 gagagccacg gggcgttcga agttgctctc gatggaaatg tgtcgcccct ggtcattcga
     4381 ttccagcgcg gcatgcatga catcgacgat gtggtatccc aactctcccg atgcgcgggg
     4441 tgcacgccca tcgcgcagcg cttgagccat atcgcttact cccagcccac ggctgttgtc
     4501 ggtataccct tcgagcaaat cgacggattc ccactggtcc gaacctgctc gcaacatctc
     4561 gaccggccca ccgaatgtgt tcgggtcagg cagcctgatc gtgccttctg atccataaag
     4621 gacaagggtg ctgcgattgg tgtcgtacac atcaaacgtc gtcgtgaggc tgcatcggac
     4681 cccattggcg aagtccagga cgctgatgac gtgagttggc gtctcaaccg gaactgtttc
     4741 acccgcacgg gggccgcttg cgatggtgcg ggtctggtaa ctcgcacttg ccgtggcggc
     4801 aactctctcg actgacccaa gcagggacat cagggcggca aggtagtagg ggcccatgtc
     4861 aaagagtggc ccgccgcccg gctggtagta gaagtccggg ttgggatgcc accgctcatg
     4921 accccggaga agcagcattc cggtgaccgc cacgggcctg ccaatcgctc cactgtccag
     4981 tagcgcacga gcggtctgga agccactgcc gaggaaggta tccggggcgc tgccaaccaa
     5041 tacgccttgt tccgctgccc gggcaagaat cgcctgtgct tcttcgcgcg tcgccgccag
     5101 tggcttttcg ttatagatgg acttgcccgc gttgacgacc tgaagcccga cctgaccgtg
     5161 cgcggcggga atggtgagat tgatgacaag ctcaatatcg gggtgggcca ggagctggtc
     5221 tacgctgagg gcctcaatgc cgaagccttg agcctgctcc tcagcccttt ccatcatcac
     5281 atcggcaatg ccgacaacct tgaggttgtc aaagcggccc gtgcaattct ccaggtactt
     5341 tgtggagata taacccgcac caataatgcc gatgccaacc gcattagttc gctctgccat
     5401 aggtcccccg cattttagta aaccgtccag ccaaaaggga tttcggacgt cgcaatagcg
     5461 tgcggaacgt cgtttggcag catgcgtgag attgagagag actgcaaggg tagaccggca
     5521 gatatgttgt taggtgggac tgcaaggtct gcggagaagc cgtcatccct attgcagcgg
     5581 tgggtaagaa aaacccgatc atattaactg tttagcccat gatcattggg tttcggttgt
     5641 cattccatgg ttcctggatg cgcatggtaa tccgccaagg caaggaaaca atgtcctgct
     5701 tcagtgcctt tgcgggacca aactgccgta gtcatgccga tattggcctt atgcagggat
     5761 tgccttatga cgtttatatt gataggatag atgcaagacc aagtcggttt cgtaaagaca
     5821 atcgtacgaa accgcatgta cgatcagggg caccacacct gtatcgcgca ttgtatttac
     5881 tcctcgtaat ctg
//
LOCUS       DASYWM010000005         4274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_431964, whole genome shotgun sequence.
ACCESSION   DASYWM010000005 DASYWM010000000
VERSION     DASYWM010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431964"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VGR29_00095"
     CDS             <1..259
                     /locus_tag="VGR29_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072016.1"
                     /translation="LSTDDLQIGHSYFMTDGIETDVVLNRVWTRAVSPLLNEYLHHHR
                     NRDEILAELTPASLLRTTVEVGPENDTAPDGQANGREPDAV"
     gene            256..1506
                     /locus_tag="VGR29_00100"
     CDS             256..1506
                     /locus_tag="VGR29_00100"
                     /inference="COORDINATES: protein motif:HMM:NF021622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072017.1"
                     /translation="MIATREVIKEHQDFRWEFSQDDLALIGGPLRNAFTITRSLVGPE
                     YILNPGSTVGVIRLPSGLQLEIQPKVPLTNLLWMLAAVEDLTAIDFKQLEDSVDLKEF
                     DQVLEIIADAFAGMVEHRLDLGLYRNYVEEEGNLTAIRGRILFAPDIEQNAVLRHRTY
                     CQYTTYSWDLPENQVIRQVARQLSGWGFSSGLTGRLVALDHQMDEVHPSRLSAGDVDQ
                     FVYNRQSEDYRPIHRYCRFFLNGASLNEEAGDTAFDGFLWNMNALFEKFITTMLRARL
                     EPRFRLKDQERSTLDVNRAVTIKPDLVLADGLIDILIGDTKYKKLGTDDHKHADLYQT
                     LAYCTALDVRAGVLIYPRHNIVREDRLAVRNSPVTLHETSVDLRGSITHIEHEIDELA
                     QRLRDWSDRGNAVTPLHAPQRTSA"
     gene            1528..2079
                     /locus_tag="VGR29_00105"
     CDS             1528..2079
                     /locus_tag="VGR29_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV2072018.1"
                     /translation="MTTKLPSHPVAATLYANFPRWVLRLNREFPHDPGRVWDAITKAD
                     EVAQWAPFRPDHDLIAHGDVWLTPTDGGEEDQPGRVLEVQSPSSLLYLWVSDHLHFDL
                     TPTDGGTGLGLGYTFDDRNSASSIAAGWHLCLAALELLLDGKEVPSVVGEKAKEYGWE
                     DLERQYAELFEENDASPEPMDSL"
     gene            complement(2511..3491)
                     /locus_tag="VGR29_00110"
     CDS             complement(2511..3491)
                     /locus_tag="VGR29_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEV2072019.1"
                     /translation="MEISGIRFVGLNAENGQKLLFTAVLILLVWILRSVLRHLVRFAH
                     KGIDDPHAEFWTRQAINIGTTLLLVLGLLSIWFDDPTRLATALGLVTAGLAFALQKVV
                     TAIAGYFVILRGNNFTVGERITMGGVRGDVIGLSFIQTTIMEMGQPPAVQGADPAMWV
                     RSRQYTGRIVTVTNDKIFDEAVYNYTRDFPYLWEEITIPISYTVDRERAEQILLRAAK
                     RHADVYGEQGQRAMDVMMRRYFMQPASVEPKVYYRLTDNWLELTVRFLTEDRGVRDIK
                     DMMSREILREFEAAGIGLASATFEIVGFPPLRIVQAGTDKQAAPNGPAGQ"
     gene            complement(3610..>4274)
                     /locus_tag="VGR29_00115"
     CDS             complement(3610..>4274)
                     /locus_tag="VGR29_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072020.1"
                     /translation="LSRLLYGARISMIVGIVPTIITLLVGASYGLASGWVGGHWDNIL
                     MRLVDVVYAFPSLLLFIIMQASLRDTGFGRMLGGLMLLFVAFAITGWNGTARLIRGEV
                     LSVREKEYVHAARAIGGSSRRIVLRHVLPNCLAPIIVSVSFSIPSYILAEASLSFLGI
                     GIRPPNASWGSMVYTSFPLITYQPIFVIMPSMLIALVMIAFAFVGDGLRDALDPQSGG
                     RR"
BASE COUNT          911 a         1330 c         1228 g          805 t
ORIGIN      
        1 cctcagtacc gacgatctcc agatcggtca cagttacttc atgactgacg gcatcgagac
       61 agatgtggtc ctcaaccgcg tctggacgcg agcggtgtca ccccttctca atgagtacct
      121 gcatcaccac cggaatcgtg acgagatcct tgccgagctg acacccgcca gtctgctccg
      181 gacaaccgtt gaggtgggcc cggagaacga cacggccccc gatggccagg ccaatggtag
      241 agaaccggat gcggtgtgat agctacacgc gaagtgatca aggaacacca ggatttcagg
      301 tgggagttca gccaggatga cctcgcgctg atcgggggcc cgctaaggaa tgcgttcacg
      361 atcaccaggt ccctcgttgg ccccgaatac atcctgaacc ccggttccac cgtgggtgtc
      421 atccgcctgc cgtcgggcct gcagcttgaa atccagccga aggttccact gacgaacctg
      481 ctctggatgc tcgctgcggt ggaagacctg actgccatcg acttcaagca actcgaggat
      541 tccgtggacc tgaaagagtt tgaccaggtc ctcgagatca ttgccgatgc cttcgccggt
      601 atggtcgagc accggttgga tctcggtctc tatcgcaact acgtcgagga agagggcaac
      661 ctgacggcga tacgcgggcg catcctcttt gccccggata tcgagcagaa tgccgtgctt
      721 cgccaccgaa cctactgcca atacaccacc tactcatggg atctccctga gaaccaggtg
      781 attcgccagg tcgcgcggca gctttccggc tggggtttct cctccgggct gactggccgc
      841 cttgttgcgc tggatcacca gatggatgag gttcacccct cccgcttgag cgcaggggat
      901 gtcgatcaat ttgtttacaa tcggcagtcg gaggactatc gcccaatcca tcggtattgc
      961 cggttcttcc tgaatggagc atcgctcaat gaggaagcag gagacacggc gttcgatgga
     1021 ttcctgtgga acatgaatgc gctcttcgag aagttcatta ccaccatgct gcgggcacgc
     1081 cttgagcccc gattccgcct gaaggaccag gagcgctcga cgctggacgt gaaccgcgcc
     1141 gtcactatca agcccgatct cgtccttgcc gacggcctga tcgacatcct gattggcgac
     1201 acaaagtaca agaagctcgg caccgacgat cacaaacacg cggacctgta ccagacgctc
     1261 gcctattgca cggcgctcga tgtccgcgcg ggtgtcctta tctatccacg ccacaacatc
     1321 gttcgcgagg accggctcgc ggttcgcaat agcccggtga cgctccacga gacttcggtc
     1381 gacctccggg gctcgattac ccacatcgaa cacgagatcg atgaactggc gcagcgcttg
     1441 cgagactgga gtgatcgcgg aaatgccgtg acaccgctcc atgctcccca acgaacttca
     1501 gcctaacctc caaaaggacg attgcccatg accaccaaac tgcccagcca tcccgtagcc
     1561 gccacactct atgccaattt cccccgatgg gttctccggc tcaaccgtga gtttccccat
     1621 gatcccggca gggtctggga cgcgatcacc aaggccgatg aggtcgccca gtgggcgcct
     1681 ttccgccctg atcacgatct gatcgcgcac ggagatgtct ggctgacacc gaccgacggc
     1741 ggcgaggagg accagccggg ccgcgtcctt gaggttcagt cgccatcgtc attgctctat
     1801 ctctgggtct ccgatcatct ccacttcgac ctgacaccga ccgatggagg cactggcctt
     1861 gggctgggtt acacgttcga cgaccggaac tcggcctcca gcatcgctgc gggatggcac
     1921 ctttgcctcg ccgccctcga gttgctgctc gacggaaaag aagtcccatc cgttgtcggc
     1981 gagaaggcga aggagtatgg ctgggaggat ctggaacggc aatatgctga gctcttcgag
     2041 gagaacgatg cttccccgga acctatggac tccctgtaag acaggaagca gcgacgagca
     2101 agaatcatca ggagccgtgg cccttgcatc cccttattca agagaatcga gatgccattg
     2161 aagccctttg ccggcaattc gtgttgcacg gctggaggtt ttcggctcaa ttgtcagcga
     2221 cgaatgcgac cccgagcggt cggatgtgga cttcatcgtg gagtatcccg agggctatga
     2281 tctggggccg tgggggggaa acaccttgaa ttgcaggagc gccaggaagc cgtcccggga
     2341 cggaaggtgg acttaatcat ggccggcgcc atccgaaagc cgcgattcat cgaagcaatc
     2401 cttccaggca ggcggctcct ctattcaccc aaaatctccg aagctgcttg acgacgttcc
     2461 gcagattcac tggatggtgt ggcacaggag tgatctctct tcgatacgta tcactggccc
     2521 gcgggtccgt tcggggccgc ctgtttgtct gtcccagcct gaacaattcg aagtggcggg
     2581 aatccaacga tctcaaacgt cgcggaggcc agcccaatcc cggctgcctc gaactcacgc
     2641 aggatctcgc ggctcatcat atccttgata tcccgcaccc cacgatcctc ggtcaggaag
     2701 cggaccgtga gctccagcca gttgtccgtc agccggtaat agactttcgg ctcgaccgat
     2761 gctggctgca tgaaatagcg acgcatcatg acatccatcg cccgttgccc ctgttcgcca
     2821 tagacgtcag catggcgctt cgctgcccgg aggaggatct gctccgctcg ctccctgtcc
     2881 acggtgtagg agatggggat ggtgatctcc tcccacagat agggaaagtc ccgggtgtag
     2941 ttgtacacgg cctcatcgaa aatcttgtcg ttcgtgactg tgacgatgcg gcccgtgtac
     3001 tggcgactgc gtacccacat ggccgggtcg gcaccctgca ccgcgggcgg ctggcccatc
     3061 tccatgatcg tcgtctggat aaagctcagg ccgatcacat caccgcgcac gccacccatc
     3121 gtgatgcgct cgccaactgt gaaattgttg ccgcgcagga tcacgaagta ccccgcgatc
     3181 gccgtgacca ccttctggag ggcgaatgcc agacccgctg tcacgaggcc cagggccgtg
     3241 gccaaacggg tcggatcgtc gaaccagatc gacagcaagc cgaggacgag cagcaatgtc
     3301 gttccgatat tgatagcctg tcgcgtccag aactcggcgt gcgggtcgtc aattcccttg
     3361 tgagcgaatc ggacgaggtg tctaagaaca ctgcggagaa tccacaccag caggatgagc
     3421 actgctgtaa agagcagttt ctgcccattc tcggcgttca agccgacaaa gcgaattccg
     3481 gatatctcca acgtggtacc tcaactttat cggtctggac cgcgagcgga cgcgggcaac
     3541 gtctcagctt gaggcatgcc gacgtcaccc gtcaaaccgg actcaagacg gtatccgagc
     3601 gaaggagcgc tatcgccgcc ctccgctctg cggatcgagg gcatcgcgga ggccatcccc
     3661 gacgaacgcg aaggcgatca tcaccagcgc gatgagcatg ctcggcatga tgacgaagat
     3721 gggctggtag gtgatcaacg ggaaactggt atagaccatg ctgccccagc tggcattcgg
     3781 cgggcggata ccaatcccga gaaaactgag gctggcctcc gccaggatgt aggacggaat
     3841 cgagaacgac accgagacga tgatcggcgc gaggcaattc ggcagcacat ggcgaagcac
     3901 gatccgtcgg gacgatcccc cgatcgcccg ggcggcatgg acgtactcct tctcccgcac
     3961 gctcaaaact tccccacgga tcaatcgcgc cgtcccgttc cacccggtaa tggcaaatgc
     4021 cacgaagagc agcatgagcc cgccgagcat ccggccaaat cccgtatcgc gcagtgaggc
     4081 ctgcataata atgaagagca acagcgacgg gaaggcatac acgacatcca ccaggcgcat
     4141 gaggatattg tcccagtgac caccgaccca ccctgaggcc agcccgtatg acgcgccaac
     4201 cagcaacgtg atgatggtcg ggacaatgcc gacgatcatg ctgatccgtg cgccatagag
     4261 cagccggctg aggt
//
LOCUS       DASYWM010000006        28763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_281734, whole genome shotgun sequence.
ACCESSION   DASYWM010000006 DASYWM010000000
VERSION     DASYWM010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 28763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28763
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281734"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VGR29_00120"
     CDS             <1..440
                     /locus_tag="VGR29_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEV2072021.1"
                     /translation="PTEAEWEFAARGGREGARFPWGNELTPTGKHRMNVWQGTFPGRN
                     TRADGHLGTAPVRSYQPSAYGLSCMTGNVWEWCADWFSPETYAVSDGTNPTGPEQGEA
                     KVMRGGSYLCHASYCNRYRVDARSSNTPDSSTGNLGFRTGWNG"
     gene            complement(638..1192)
                     /locus_tag="VGR29_00125"
     CDS             complement(638..1192)
                     /locus_tag="VGR29_00125"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2072022.1"
                     /translation="MTLGAVLFDLGDVIMAEETEEKVDDVTQRADLHPGMADVLKWLH
                     GRSVPMGLLADTRDGTYRNVLDMHGPFDLFDVFAISDHLGVVKPDRQMFVHALEGLGI
                     PESDWGEVAMVGNNLARDIRGANALGLTSIWLVWNQRYPCISADDAEMPDYQVSTAAG
                     LQNLLDALLSGSDTGGYAHLQPFP"
     gene            complement(1216..1980)
                     /locus_tag="VGR29_00130"
     CDS             complement(1216..1980)
                     /locus_tag="VGR29_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2072023.1"
                     /translation="MIAPFLWMLSTAFKGGGAIFDYPPSWISSPVTLENFREVWTVVP
                     FGRYLFNSLFIAICVTIGEVLTSALAAYAFARLRFPGRDALFLMYLATLMIPGQVTVI
                     PNFILMRYLGWINTYQGLIIPTAFTAFGTFLLRQYFLSIPRELEEAARVDGAGYFEIW
                     WRIIIPLASPAIATLGVFSFMGAWNSFLWPFIMVSASEMRTLTVALRSLQTQYATEWG
                     MMMAGSLIAMLPMLAIFLSAQRYFVRGIATTGFGGR"
     gene            2173..3768
                     /locus_tag="VGR29_00135"
     CDS             2173..3768
                     /locus_tag="VGR29_00135"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEV2072024.1"
                     /translation="MISTDYTTAPGRLTRSDDIWVRLIVFLGGMSSIGTEIAATHLIA
                     PYFGESTFIWATVIGLTMTFLAIGYFLGGRVAHRYPRAWLLYFTTAVAAIAAGFLPFF
                     SRPILGASLDAFENVEIGAFYGALIGVLLMLAVPITLLGFVTPYAIRLRLEHIEDAGN
                     TAGTVYALSTVGSIAGSFLPVLILIPWIGTTRTFLVLSLLLLVPSLVGLIRLRAVRPA
                     LAAVTLTGAMIAANLWVSQQSVRPAEYGTLIYETESSENYIQVTKRGDQYLLSLNDGH
                     AVHSIYDPTKLLTGSPWDYFMAGPLFIPREGPAQIDNALLIGLAGGTASKQITAGYGP
                     VPIDGVELDPEIVRVGREYFDMKEPNLNVIINDGRYVLRTTDTEYDLIAVDAYQQPYI
                     PFQLTTREFFQEVSDAMTDDGVAVMNVGRTATGFRLVDVIASTMKDVFPNVYLIDTER
                     YANTMVIATKSPSDIGTFTQSVARQPAGSPIRIVGQRALETRNVREVTEVATVFTDDH
                     APVERVVDQIIIDEALIEEGSND"
     gene            3761..4606
                     /gene="pgeF"
                     /locus_tag="VGR29_00140"
     CDS             3761..4606
                     /gene="pgeF"
                     /locus_tag="VGR29_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEV2072025.1"
                     /translation="MTDSSLSSTINRPAITPLRSQLLSSVPGVIHGLTHRVEGMGRAN
                     GNIGYSGERDRADAWEMRQQWCEAIGVEPARMVLVGQVHGNGVLEAQPDHAGAGAAPG
                     SAQVGYADALMTDRAGLVLTTLHADCLPILIVDPERPAIAAVHAGWRGTVLDVAGATI
                     RAVQERYNSDPAELLVYLGPAIGGCCNEVGSEVTGAWREQVRDLGPLAELAVTKPGIK
                     EHFDVPRANTLLLQRAGVQPENMEVSATCTQCNTDDWFSHRGHGPSAGRQAALIMLTT
                     AGDAT"
     gene            4603..5301
                     /locus_tag="VGR29_00145"
     CDS             4603..5301
                     /locus_tag="VGR29_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV2072026.1"
                     /translation="MTCAVPLDLERRAATVRLQVEAAATRADRDPGEITIVAVSKTFP
                     RSYVEAAYHLGFRAFGENRVQEIRTKYEEPLPADARIHLIGALQTNKVRQAVRVVNRV
                     ETVDRIALVETLAKELPRQGVTCSVLIQVNIAGETQKSGCRPEEAEKLLSQIANVPEL
                     SCDGLMTMAPYVDDSETVRPVFTELRTLRDHLQQVSGLALPDLSMGMSGDFRVAIEEG
                     STHVRLGRALFGDR"
     gene            complement(5523..8330)
                     /locus_tag="VGR29_00150"
     CDS             complement(5523..8330)
                     /locus_tag="VGR29_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEV2072027.1"
                     /translation="MITAAVIVLALIVLMFVTAPFWINWLWFGSVGYRSVIVTNYNAQ
                     ALLFLAAAVIAGLIFYVNMRLALRNTRSPQLAEEGRVSRASRGVIRGLTVLGSLIIAL
                     VAGVLATQRWQDVLLALYGGSFGIEDPTFGRDAGFYVFLLPVLRALETGLLALLIVTI
                     LAVALVYLIRLGIRFRSWGDVPFVALRHLSGLISAVLLVVAMRYLLNTFELVYSSRGV
                     VTGPGFTDVNIVRPLNWLMALASALVAIGLLSGFILRNPKWLAGLLGSWFLLAVVVTP
                     LLPMAVQRVLVEPNEFSREENYIERNIEMTRAGFALNDVQAYDVTGQEEIDISELSAD
                     EPPLDNVRIWDYRVVGPVFQQVQSFVPSYEFPDVDVDRYEIDGEPVQVLVGVRELNLD
                     GLAENRRTWTNTHLVYTHGYGVVVSPVSEVSGDGWPPFIVSGIPMEGPEELALDRPEV
                     YFGETDLRWIILFTDQTETTGLVEAGDAEVEGFQGEVAGSVSLGNPVTRGLAALTLGD
                     RNVFLSSQLTGDSRLVLERNIVERAKKIAPFLDYDQDPYAVIADGRLYWVMDAYTTTS
                     RFPQATRFDGTNYIRNSVKVVVDAYDGTTIFYRTGIEDQIADAWGDVYPDLFTPITEA
                     PASLSAHFRYPERQFVVQSEVWSDYHMDDARTWYDGDDKWTVAEETIDGEIQQVEPFF
                     VSQPLPGETESVFALTVPFTPGGQQTRQNMTAWFAGTADETGDTTLRLYRYPRQVTVY
                     GPRQIEAQINQDPDISQQITLWGQGGSEVIRGNMLVIPVNDAILYVQPLYLQATGSSA
                     SAPRLARVIVATNDQVVMRPTLPEAIAALADPEAESVDQIEQDPDAAIAQAEEASQPG
                     SAPQGTPVAEPSGDVPTGLAAMSEEELAAEALVTLDRAEQAQQAGDWETYGREQERLR
                     RILEIMAGSEVVPDASPEAGS"
     gene            8560..9447
                     /locus_tag="VGR29_00155"
     CDS             8560..9447
                     /locus_tag="VGR29_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2072028.1"
                     /translation="MQPSTPSCVPRPRAISVQSLGSGSSGNAFLITTGTDTLLIDCGV
                     GIRTLTASLRKRNIAINEISAICITHEHSDHIRTLPKVMHEGATFYSTRGTASRSALP
                     MERHQRAIEDVPVWIAGVAIWSLPVAHDAMEPCGFMAELPDGSRVTMLTDLGSWQESL
                     REFVRASDLIILEANHDEEMLRLGPYPTYLKRRVASNTGHLSNRHCGLALGEALRGST
                     HHPQIWLAHLSEHNNRPDLAEETVRSLLAERELDLAVTALPRRSPSEIWTPGERSGTP
                     QTFNRMRPQKVEQLGFDLF"
     gene            complement(9604..9978)
                     /locus_tag="VGR29_00160"
     CDS             complement(9604..9978)
                     /locus_tag="VGR29_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072029.1"
                     /translation="MDVIELLHNRLATTVMLFFLFVGLWGLFEYARGGALGGNVAGAL
                     IIGQVLVVIQVGFGVVLLALGTAPADSTHYLYGLTAVAIMPFLWTYTRDRAPRQGLLL
                     YSLVALFIFGLAIRGMTTGGAS"
     gene            10329..10859
                     /locus_tag="VGR29_00165"
     CDS             10329..10859
                     /locus_tag="VGR29_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEV2072030.1"
                     /translation="MESEPRDYQEVMDNALQLVYSHHHRLVSQLMPEAFRPLTLDQLR
                     NGPLGQDLLRLARLARGQIREPKEQVLDAIDSVLQLLFWPAAAEDYSVNRGFWDTDLG
                     RMLALAKYRAYEPNELVSIGNAAQQLGVTRPTVYRWMDDRTLSYVRDDMSGRTFVVRR
                     DIDKLKRVAQEFEGQI"
     gene            complement(11199..12416)
                     /gene="alr"
                     /locus_tag="VGR29_00170"
     CDS             complement(11199..12416)
                     /gene="alr"
                     /locus_tag="VGR29_00170"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV2072031.1"
                     /translation="MGIRDIVESWQGRPTFAVVDLDALATNIKTLRAHVRPEASILAV
                     VKANGYGHGAVPVAETVLENGAYGVAVATVDEGAQLRKAGISAPILAMGPMGAAERGR
                     AVGLDMAIVMSDVPFAAGLARSVRMHQRTEPIKVHLKVDTGMRRFGVPPESVVESARA
                     IMQHPELKLEGLMTHLACADELDLTSAHEQVNVFDRCVGMLSEAGIEIPVHHVANSAT
                     TVQFSQYHKNLVRSGLALYGVRPAPHIPLPGGERAMKQILTIHGMVTRVIPLAPGDRV
                     GYGGAWVAKEKSRGALVPIGYADGYLRSLSSKGWMTVSGERANVIGRVCMDQTILQMP
                     DSLPGETRQPVVIVGNGTDETPGAPTLETLAEMAGTIPHELMTGLAPRLPKLYIKGGR
                     LFAVDDLEGYRRV"
     gene            complement(12416..12904)
                     /locus_tag="VGR29_00175"
     CDS             complement(12416..12904)
                     /locus_tag="VGR29_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV2072032.1"
                     /translation="MSRQNDASEPVVAEIMRKDVPAVAPEESVARVAKLMVDYDVAGV
                     AVIDNDEIIGIITESDIVAREADVDAPTPVPFLDAIFVADAGRPYEDDIRRALAINAA
                     MLMSSPVVSIRHDATLHEVASIMIERDVHPLPVVDQDNRYVGIVSRKDLVRVIAELEN
                     RA"
     gene            complement(13011..14045)
                     /locus_tag="VGR29_00180"
     CDS             complement(13011..14045)
                     /locus_tag="VGR29_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HEV2072033.1"
                     /translation="MATEGPIPKPELGHNASWADDTFARRVRPGRKIGTHKWEVGGVI
                     VIAGSPSFIGAAYLASRAAGRAGAGIVYLASGRSVGVALAGAMPEVAHIVLPETDAPG
                     SARRAVERLEPHLKKAKSVVIGPGLGDDELTDHLLSALFGFGGKNAQIGSNIGFGGPG
                     LVTTNARDVANSPIFGHEHIKLVIDADALTWLSKQEEWWTHMPQGRAVLTPHPGEMAR
                     LTGLSTEEIVADPSHVAREYAEKWGQAVLMKSAYSAASDGKATFVAEDAPTSLATAGT
                     GDVLSGTVGALLAQGLTPLDAAGLALFAGAKAARALEYRFGDLGVIATDMPDQIAGVL
                     GKLGTGNESR"
     gene            complement(14069..14587)
                     /gene="acpS"
                     /locus_tag="VGR29_00185"
     CDS             complement(14069..14587)
                     /gene="acpS"
                     /locus_tag="VGR29_00185"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV2072034.1"
                     /translation="MPERPRWREYQPPVLEEREGEGWYVPEFDPEREGGVAVGTDIIE
                     IARIQRSLNDFGDRFLKRVYTEREREWYGTRVNELAARFAAKEATSKALGTGIIGIRW
                     REMEVLPNRRGKPVLVLHGRAAERARQLGLTSFSVSLTHSRTDAMAFVVGTGEGSGLP
                     SGAVLDHVAENS"
     gene            complement(14587..15825)
                     /gene="tgt"
                     /locus_tag="VGR29_00190"
     CDS             complement(14587..15825)
                     /gene="tgt"
                     /locus_tag="VGR29_00190"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV2072035.1"
                     /translation="MIDSVNRPVSPGLRGQSSVFSGRFSFELLREDRETLARRGRLTT
                     TRGTVETPVFMPVGTQGTVKTLHPAEVRETGAQIILANTYHLMLRPGLEIIEEAGGLH
                     TFMRWNRPILTDSGGFQIFSLANNARVTEEGVRFASHIDGSKWEMTPERVVELQMGFG
                     SDIMMQLDHLIGLPAEHTAIAAATERSARWLERAITHYTDRNGSASRSVLFGIQQGGM
                     EADLRRESARRLASMDVAGCAVGGLSVGEPKPVMAEMLEVSTPELPRDKPRYLMGVGS
                     PEDLWNGVARGIDMFDCVLPTRAARNGALFTPDGRISIRNASWKHIHEPVDQTCDCVA
                     CTEFTAAYLHHLFRADEVLGLRLASVHNLRFLARQMDEIRAAISAGTFAKVHGAFADR
                     YRPVANPSPVGVTRPKKEQR"
     gene            complement(15832..16560)
                     /locus_tag="VGR29_00195"
     CDS             complement(15832..16560)
                     /locus_tag="VGR29_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEV2072036.1"
                     /translation="MFGVGPQEMLLIGLAALLIFGPGKLPEVMGQAGKLYKDFRNMTS
                     ELTGEFEKTVAEAREMGNQLTGDLGPMQKQVDSVTRSVQRDLGKGTKGTATSKKSAGT
                     TSSSSASAKKSGTTGSRSSGTKTTSSKSTSSSRTTPSKTETKADAKTSETIKKTAVAP
                     VATKEDPYSDISVFAPSEPERTRRARRATPSVISDRTPRQIAASTAADVVESAGSAGP
                     APVDDAIARARQRRRAAGYAQRSA"
     gene            16804..17391
                     /gene="pth"
                     /locus_tag="VGR29_00200"
     CDS             16804..17391
                     /gene="pth"
                     /locus_tag="VGR29_00200"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV2072037.1"
                     /translation="MFDRDHTKVVIGLGNPGRKYAGTRHNLGFLVVDELAKRTSAPAS
                     KQRMRAEITETRPDNSRLVLAMPQTYMNDSGISVREIVRWYKAPLEDVLIVVDDLDLP
                     FGQIRIRPKGSAGGHNGLKSIFHELGSQEIPRLRIGIGRGGNHTISHVLSRFSPEEQQ
                     HLPEIIEQAADAVEHWMRHGMLATMNVTNGSSSPL"
     gene            17437..18312
                     /locus_tag="VGR29_00205"
     CDS             17437..18312
                     /locus_tag="VGR29_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV2072038.1"
                     /translation="MTPEQEQEATAILGVVGAGTMGSGIAQVAAQSGYNVVLIDTEEE
                     MLDRGLARITSDVDSLVERERLSSENRDEVLSRIEGATTLERLDPCELVIEAVTEDRE
                     IKAGVFKQISESVDPETIIASNTSSLSITELAHCVPNPQRVVGMHFFNPATRMKLVEI
                     VAGRATSEDTIAVAIATAMQMGKTPVVVGDSPGFVANRLMIPMINEAIFTLNEGVASC
                     DDIDQIMQLGAGHPMGPLALADLIGLDVCLSIMEALHHEFGDDKYRPAPLLRQMVAGG
                     QLGRKSGSGFYQYQA"
     gene            18324..18770
                     /locus_tag="VGR29_00210"
     CDS             18324..18770
                     /locus_tag="VGR29_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEV2072039.1"
                     /translation="MGTGQELMAASGRSRPIRVLVAKPGLDGHDRGAKVMARAFRDAG
                     FEVIYTGLFQTPQMIANAALQEDVDVVGLSILSGAHMSLFSPIMDALRDVGREDVLVV
                     AGGTIPDEDIPTLNELGVAKIFGPGTPLQDAVAYIREHAPDRVDLP"
     gene            18770..19756
                     /gene="meaB"
                     /locus_tag="VGR29_00215"
     CDS             18770..19756
                     /gene="meaB"
                     /locus_tag="VGR29_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEV2072040.1"
                     /translation="MTGTPHRASLSERVLAGDRRALARLLTSIENDDPLGEQAMEKLF
                     PHTGSAHLIGITGPPGGGKSTLVNEMIRAYREMPKSVAVIAIDPSSPLTGGAALGDRI
                     RMQEWHADVGVFIRSMASRRFGGGLARNALAVAHAFDAAGFDQIIIETVGTGQDEVTI
                     ASLAMTTLLVQVPGTGDGVQTLKAGALEIGDVLVVTKGDRPESSDLARDLRNLRQLGM
                     AAGSELSRWDTPVIKTSAARREGIDSLMNAIAAHRSWLQETGEMESRVQTMINTEITF
                     RVQDALLQEIRRPVSGDGGMQRLVERVAAREVTPHQAADEMLARLRHNDTAP"
     gene            19911..20657
                     /locus_tag="VGR29_00220"
     CDS             19911..20657
                     /locus_tag="VGR29_00220"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV2072041.1"
                     /translation="MSGHSKWSTIKRAKGAADAKRGQLFTKLAREITVAARTGLPDPE
                     ANSRLRLAIQKARGENMPKDNIDRAIARAAGADGGSQYDEIYYEGYGPGGIALMILTM
                     TDNRNRTVGEVRAALTRSGGSLGENGSVSWMFDQAGLITIPVDGHDPDEIQLVAIDAG
                     ATDVQVEDTAIEIYTDPAELHKIAEQFTEAGYEPQAVELTMKPKTMLQPDEDSAVKAI
                     RLVEKLEDLDDVQTVYTNLDISDEVLAQVS"
     gene            20686..21219
                     /gene="ruvC"
                     /locus_tag="VGR29_00225"
     CDS             20686..21219
                     /gene="ruvC"
                     /locus_tag="VGR29_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEV2072042.1"
                     /translation="MSVTSGSRSEPITLGIDPGTARLGYGLIRGGITPSLIDAGVIET
                     WPDQDMPGRLVTLYESVRELIQEFEPQMLAIEKLFFARNVTTAIAVGQARGVVLLAAA
                     QSGITVSEYSPSEIKHAIAGYGKADKNQMQEMVRMILHLRNVPQPDDAADALAIAICH
                     SQTASYEVRSLRHGNRE"
     gene            21216..22028
                     /locus_tag="VGR29_00230"
     CDS             21216..22028
                     /locus_tag="VGR29_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV2072043.1"
                     /translation="MIKTQRLDHAMVERELADTRSRAKALIMAGDVLVNGQKEMRAGH
                     NVGPADTLTLRAKPRFVSRGGEKLDHALGVFGMDVNGMTAADLGSSTGGFTDCLLQRG
                     ASKVYAVDVGYGILDERIRTDPRVISMERTNARYLTELPEPVDIVVIDVSFISLSLML
                     PVAARILEPEGICVPLIKPQFEAGKENVGKRGVVRDPAVHEMVLRNAIGHAGKNNLSI
                     LGLIASPLRGPNGNVEFLASLIRDASVESPDVDRLIASAMAEAKALTEEATS"
     gene            22025..22759
                     /gene="larB"
                     /locus_tag="VGR29_00235"
     CDS             22025..22759
                     /gene="larB"
                     /locus_tag="VGR29_00235"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEV2072044.1"
                     /translation="MTDPFADLLAALNGTAPEPVSPYLRVDPSRAARTGVPEIVFAER
                     KTVDEVLVALRSLAQSNGRALASRLTSEQLDHLGSFSEPGFTLDIDTEARMAIMAQPG
                     MQDPQIYGRIAVIAAGTSDRSVAREATLTARMMGCEVIEIHDVGVAGLHRLVQPLRSA
                     VASGIDAIVVAAGMDGALPSVVTGLVDVPVVGLPVSVGYGAGGNGEGALLAMLQSCAP
                     GMAVVNIDNGIGAGSMAALIARRKTQ"
     gene            23116..24255
                     /gene="rpoD"
                     /locus_tag="VGR29_00240"
     CDS             23116..24255
                     /gene="rpoD"
                     /locus_tag="VGR29_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV2072045.1"
                     /translation="MDTSLITKGKDQGYLLSEEIIAAFPNADEQSEPLEEFYSALVAE
                     GIDVLDRHPKPQKTARVVRSKTDARAGTEDPLAGGVGDSVRLYLQEIGETSLLSMEEE
                     VWLATRMERGKAAEARLQERGLNEEDRTDLELDRKDGDAARAHLIQANLRLVVSVAKK
                     YVGRGLSFLDLIQEGNIGLMKATDKFDYHRGFKFSTYATWWIRQAITRAISDQSRTIR
                     LPVHVGETINRVKKTSHRLQQIFEREPTQEEIARAMSITDDKVRQVFDVSRHPVSLEA
                     PVGSDGDAFLGDFIEDDTMPAPLELASQQLLKAQIGSALDRLSERERRIVVLRYGLED
                     GRFRTLEEVGRDFGITRERIRQIEAKALRKLRHPDSSQPLRGYLD"
     gene            complement(24500..26005)
                     /locus_tag="VGR29_00245"
     CDS             complement(24500..26005)
                     /locus_tag="VGR29_00245"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEV2072046.1"
                     /translation="MRIVTGGMAQETNTFQRVATTMDDFCRPGFGFFARGQDILGLEG
                     TGTVYGGVISEAKASGVDLVPTVYAECMPGGRVSRHAFDTVRDEILDGIRAALPVDGV
                     LLVLHGAMALEDVDDAEGPLIESVRDLVGADVPIVAPLDLHTNLSDSMVESADALVGY
                     KEYPHTDMPETGAQALRLLVNIIRDGTRSTMKHTRLPLIVPNQSMVTTWQSPLKSAID
                     RAREIEMQPGVLAATVLGGFPFADVPFAGVATIVVTDNDPALARAYAYELADICWDRR
                     SDFTVNPTPVSEAIAEAMEAAPGSVYVLADVADSGASGTASDGTVVLKGLLDAGARSA
                     AVAQIMDAEAVAACIDAGVGSDVALTIGGRQDGLHGPPVEVRGKVRLIHEGSFMLAGP
                     MDAGTPASRGRTVVLEIGGHDGIEVQLTELRGHPSDLNHFRAFGIEPTQRRILVLKSA
                     AHFRAAFEPIATKVIEVDAPGISSPKLTNFDYQRLRRPIYPLDPDTVWSRS"
     gene            complement(26231..27205)
                     /locus_tag="VGR29_00250"
     CDS             complement(26231..27205)
                     /locus_tag="VGR29_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072047.1"
                     /translation="MATGDSVRTRKPVPVASTETVPTATPSRRLASDDEPLALMGTRQ
                     RRHRSSIRRFISTSPLGTLTTFILVAAIVIAILGPFIGTGDPEEISPIAMFASPSGDF
                     PMGGDYLGRSVLARLVYGLRISLALAAASALLAASVGTLLGLVSGYFGGVLDELVMRF
                     TDILFAFPTILLGLLVAMVLNPGVKSVIFTIVIATIPVFARVARGPTLSVREAEYTIA
                     ARVIGATPPRILFRYVLPNVSTPLLVQLAFTLSGALIAEGALSFLGLGVQPPMPSLGS
                     LLRDGKTYMEIAPWTMLFPGVTLAVAILAINLLGDELQSFTDPRLRNK"
     gene            complement(27254..28201)
                     /locus_tag="VGR29_00255"
     CDS             complement(27254..28201)
                     /locus_tag="VGR29_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072048.1"
                     /translation="MSRYIAKRTVMALITIFGVATIVFVAMRLVPGGFAAALLGPNAV
                     QQPEVVAAIEEKYGLDQPVFVQYTLWLGNALRGDLGESLRTHSSVTSEIVRRSLITIE
                     LTLLATLMSLVIGIPAGVIAAIRRNSVADITTRTVSLVGLSIPDFVLGTILIYVVSTR
                     HIGLPISGYISPSDGIIDHLRSMVLPTFTLGLITTSVVMRVIRSSVVEVLGEPYVITA
                     RAKGLHPRDVALHHVVRAALIPTVTIVGINMGYLLGGAVIVEELFSLPGLGRYALTGI
                     LNRDYPVAQGTVIVGALMFVLANLAADIAYAYLDPRIKY"
     gene            complement(28367..>28763)
                     /locus_tag="VGR29_00260"
     CDS             complement(28367..>28763)
                     /locus_tag="VGR29_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072049.1"
                     /translation="IVQRDLSTFVNEYAVEGTAQLAISWWAGYSDPYLILLENGSSDF
                     APVLGITDPTVDELIAKAASTVDPEERLEVLYQLEDAIATIAGFQPLVTRDNFISYRK
                     DLVSNLTFAEGEGFGLPLWHRLDDVTVSQ"
BASE COUNT         5993 a         8874 c         8434 g         5462 t
ORIGIN      
        1 tgccgacgga agcggaatgg gagttcgccg cacgaggtgg tcgggaggga gcgcgcttcc
       61 cgtggggcaa tgaactaacg ccaacgggca agcaccggat gaacgtctgg cagggcacct
      121 tcccggggag aaacacccgg gcggacggtc atcttggcac ggcaccggtg aggagctacc
      181 aaccgagtgc ctatggcctc tcttgcatga ccggcaatgt ctgggaatgg tgcgccgact
      241 ggttttcacc ggagacctat gcagtgagcg atggaacgaa tcccaccggg ccggagcagg
      301 gtgaggcaaa ggtcatgcgc ggggggtcct atctctgcca tgcctcctac tgcaaccgct
      361 atcgcgtcga cgcccgcagc tccaacaccc ccgattcatc cactggaaat ctcggctttc
      421 gcacggggtg gaacggataa ggaggggttg cattccggtt cggatgtcgc cgaggtgctg
      481 ttgaggtttc gtcaggctga ggaccacgga agaggagtgc ggcccttccg ccatgaagac
      541 catgctttgc ggataagcta cgttgtgcga aaaggctgcc ttagacctcg aagtgcggaa
      601 caagggcaac accctcccaa catcaggtag cgtcgtctca gggaaagggc tggagatggg
      661 catacccgcc tgtatcgctc cctgacaaca gcgcatcgag cagattttgg agtccggcgg
      721 cggtactcac ctgataatcc ggcatttcag cgtcgtcagc actaatgcat ggatagcgct
      781 ggttccacac cagccagatg ctggtcaagc cgagcgcgtt tgcgccacgg atgtcccgag
      841 cgagattgtt tccaaccatt gccacctcgc cccagtcaga ttcaggtatg ccgaggcctt
      901 ccagtgcgtg cacgaacatc tgacgatcgg gcttcacgac cccgagatga tcggagatcg
      961 caaagacgtc gaagaggtca aacgggccgt gcatgtcaag cacgttgcga taggttccat
     1021 cccgcgtatc ggcgagaagg cccatcggca cgctacgccc atgcagccac ttgagaacat
     1081 ccgccatccc cggatgcaag tccgcccgct gcgtgacgtc gtcgaccttc tcctcggtct
     1141 cctccgccat aatcacatcg ccgagatcga acaatactgc acctaacgtc acgtcagccc
     1201 gaccctcttt tatgtctacc gaccgccaaa gccagttgtt gcgatgccac ggacaaagta
     1261 ccgctgtgcg gaaaggaaga tagcaagcat cggcagcatg gcaatcagtg atcccgccat
     1321 catcatgccc cactcggtgg cgtactgggt ctgtagcgac cttagggcca ccgtcagcgt
     1381 cctcatctcg gaggcgctaa ccatgatgaa gggccataga aaactattcc acgcgcccat
     1441 gaagctgaag acgccgagcg tcgcgatggc aggactggca aggggtatga tgattcgcca
     1501 ccagatctcg aaatatccag cgccatcgac ccgggcggcc tcctccaact cgcgcggtat
     1561 cgacaggaag tactgtcgga gcaggaaggt tccaaatgcc gtgaaggccg tcggaatgat
     1621 caatccttga tacgtattga tccagccaag gtagcgcatc aagatgaaat tcgggatgac
     1681 cgttacctgg ccgggaatca taagcgtcgc aagatacatc aggaagagcg cgtcacgccc
     1741 ggggaatcgc agccgggcaa acgcataggc cgccaatgcc gaggtcagga cctcgccgat
     1801 cgtgacacag attgcgatga agaggctgtt gaagagatat cggccaaacg ggacgaccgt
     1861 ccacacctca cggaagttct caagagtcac tggactggag atccatgatg gcggatagtc
     1921 aaagatcgcc cctccgccct tgaacgcagt agagagcatc cacaagaatg gtgcaatcat
     1981 cagcaccgcg ccgagcgccg gttacgtcca gctcggcgag cagccggtcg aggaagccaa
     2041 caccagcgac tgtaacaatc gaacatggtg ttcaacggat cgacagtagc cgcagggcct
     2101 tttatggata tgcgcgggcg cgtctatact ggccaccatg aatagactcg tctttcgttc
     2161 ctggcaggct ttgtgatctc aaccgactac accacagcac ccggacgtct cactcgtagc
     2221 gacgacatct gggttcgact cattgtcttt ctcggcggca tgtccagcat tgggacagag
     2281 atcgcagcaa cccacctgat agccccttac ttcggggaat caacattcat ctgggctacg
     2341 gtcatcggtc tcacgatgac cttcctcgct attggctatt tcctgggcgg tcgtgttgcc
     2401 caccgctacc cacgcgcgtg gttgctctac ttcacgactg ccgttgccgc catcgcggca
     2461 gggtttctcc ccttcttctc ccggccaatt ctcggcgcct ccctggacgc gtttgagaac
     2521 gtggagattg gcgcattcta tggtgccctt atcggggtgc tgctcatgct tgctgttccc
     2581 atcacgctcc ttggctttgt cacaccgtat gcaattcggc tccggctcga gcacatcgag
     2641 gatgcgggga acaccgccgg aacggtctat gcgttgagca ccgtggggtc gatcgctggc
     2701 agctttctgc ccgtcctgat actcatccca tggatcggca cgacgcgcac gttcctcgtg
     2761 ctcagcctgt tgctcctggt cccgtccctc gttggactca tacgcctccg cgcggttcgc
     2821 cctgcacttg ctgcggtcac tctcacgggc gcaatgatcg ctgccaacct gtgggtgagc
     2881 cagcaatcag tccgtcccgc ggaatacggg acactcatct acgagacgga gtcgtccgag
     2941 aattacatcc aggtgacgaa acgcggcgac cagtacctgc tctcgctcaa cgacggacat
     3001 gcagttcatt cgatctacga cccgacaaag ctgctcaccg gaagtccatg ggactacttc
     3061 atggcgggcc cgcttttcat cccaagggag ggaccggccc aaatcgataa cgcgctcctg
     3121 attggcctcg cgggtggcac tgccagcaag cagatcaccg ccggctacgg ccctgtcccg
     3181 atcgacgggg tggagctcga cccggagatc gttcgcgttg gtcgcgaata cttcgacatg
     3241 aaggagccga acctcaatgt catcatcaac gacggccgat atgtgctccg gacgaccgat
     3301 acggagtacg atctgatcgc agtggacgct taccagcaac cctacattcc gttccagctc
     3361 actacgcggg agttcttcca ggaagtgtcg gacgcgatga ccgatgacgg cgtggcggtg
     3421 atgaacgtgg ggcgcacggc gaccggcttc aggctggttg acgtgatcgc ctcgaccatg
     3481 aaggacgtct ttccgaacgt ctacctgatt gacacggagc gctatgcgaa cacgatggtg
     3541 atcgctacca agtctccctc tgatatcggc acgttcaccc aatcggtcgc ccggcagccg
     3601 gcagggtctc ccatccggat tgttggtcag cgcgcgctgg agacccgaaa tgtccgcgag
     3661 gtaaccgagg ttgccaccgt ttttaccgac gaccatgcac cggttgaacg tgtcgttgac
     3721 cagataatca tcgatgaagc actgatagaa gaaggttcga atgactgatt ccagcctcag
     3781 ctcgaccatc aacagaccag ccatcactcc gctccggagc cagctcctct cctcagtgcc
     3841 cggcgtcatt catggcctca cacaccgtgt cgagggcatg ggaagggcaa atggcaacat
     3901 cggctacagc ggagaacgtg atcgcgcgga tgcctgggag atgcgccagc agtggtgcga
     3961 ggctatcgga gtggaaccgg cacgtatggt gctcgtcggc caggtccacg ggaatggtgt
     4021 gcttgaggca cagcccgacc acgctggcgc cggggctgct ccagggagcg cccaggtcgg
     4081 ctatgctgat gccctgatga ccgatcgcgc cggtctcgtg ctgacgacgc tccacgccga
     4141 ttgcctgccg atcctgatcg tggatccgga gcggccggcg atcgctgccg tccacgcggg
     4201 ttggcgtggc accgtgctgg atgtcgccgg tgcgacgatc agggcggtgc aggagagata
     4261 caactctgac ccggcagagc ttctcgtcta tctcggcccc gccatcggtg gctgctgtaa
     4321 tgaagttggt tcggaggtca ccggggcctg gcgcgagcag gtgcgtgatt tgggacctct
     4381 ggcagaactt gcggtgacga agcctggaat caaggagcat tttgatgttc cccgggcgaa
     4441 cacgctcctc cttcagcgag cgggggttca gccagagaac atggaggtct cggcgacttg
     4501 cacccaatgc aacactgatg actggttctc acaccggggg cacggcccgt ccgcgggacg
     4561 gcaggccgcg ctgatcatgc tgacgacggc aggagacgca acgtgacgtg tgccgttccg
     4621 ctggatctag agcgccgcgc ggccaccgtc cgccttcagg tcgaggcggc cgcaacgaga
     4681 gcagaccgcg atcccggcga gatcaccatc gtcgccgtga gcaaaacctt cccccgctca
     4741 tatgtggagg ccgcgtacca ccttggattc cgcgccttcg gtgagaatcg cgtgcaggag
     4801 attcgcacga agtacgagga gccgcttccc gccgatgcgc ggattcacct tattggtgcg
     4861 ctgcagacaa ataaggttcg ccaggcagtc cgtgtcgtca atcgcgtcga gaccgtcgat
     4921 cggattgcgc tggtggagac actcgctaag gaacttccca ggcagggggt cacctgctca
     4981 gtgttgatcc aggtcaatat cgctggggag acgcagaaat cgggatgtcg accggaggaa
     5041 gccgagaagc ttttgtcgca aatagccaac gttccggaat tgagctgcga cggcttgatg
     5101 accatggcgc cctacgtcga cgactccgag acggtgcggc cggtcttcac tgagcttcgc
     5161 acgctccgcg atcacctgca gcaggtaagc gggcttgccc ttccggacct ctccatgggc
     5221 atgagcggcg atttccgcgt tgcgattgag gaaggctcca cccatgttcg tcttggtcga
     5281 gcgctattcg gcgatcggtg acatgccgcg aaccatgtgt gacacctctg tcattcgagc
     5341 cggaaggcga taatgcctaa acaagcctga ggccagactg ttctgcactt cggaccatcg
     5401 ccgcgccact acaggagacg gtagaggcac gaccttccac gcttaatccg tcgccctgag
     5461 gtgcgtcacg tgccttcaag acaggcgacc caagtctgga ccgcccaatc atcccggatc
     5521 tttcaggatc ccgcctcggg cgatgcatcc ggcacaactt cgcttcccgc catgatctcg
     5581 agaatccggc gcaaccgctc ctgctcccgg ccgtaggtct cccaatcgcc agcctgctgt
     5641 gcctgctcgg cgcgatccag cgtcacgagc gcttcagcgg caagctcctc ttcagacatg
     5701 gcagcaagac cggtcggtac atcaccactc ggctcggcca cgggcgtacc ttggggcgcc
     5761 gagcccggct gcgaggcttc ctctgcctgc gcgattgcgg catccggatc ttgctcgatc
     5821 tgatcgacac tctccgcttc cggatccgcc agtgcagcaa ttgcttccgg caaggtggga
     5881 cgcatcacaa cctgatcatt ggtggcaacg atgacacgcg ccagccttgg tgcgctcgcc
     5941 gatgagccag ttgcctgcaa atagagcggc tggacataga gaatcgcgtc gttcaccggg
     6001 atcaccagca tgttgccgcg aatcacttcc gaaccaccct ggccccagag tgtgatttgc
     6061 tgggagatgt cgggatcctg gttgatttgt gcctcaatct gtcttgggcc gtagacggtc
     6121 acctgccttg gatagcggta cagacgcagg gtggtatcgc ccgtctcatc agctgtaccg
     6181 gcaaaccagg cggtcatgtt ctgtcgcgtc tgctgtccgc caggcgtgaa cggtacggtc
     6241 aatgcgaaga cggattcggt ctcgcccggc agcggctggc tcacgaagaa cggctcgact
     6301 tgctgaattt cgccgtcgat agtctcctcc gccactgtcc acttgtcatc gccgtcgtac
     6361 caggtccgag catcgtccat gtgatagtca ctccacactt ccgactgaac gacaaactgc
     6421 cgctctggat accggaaatg ggcgctcagc gatgcaggag cttcggtgat gggtgtgaag
     6481 agatcagggt acacgtcgcc ccatgcatcg gcgatttggt cctcgattcc agtccggtaa
     6541 aagatcgttg tgccgtcata cgcgtcgacg acaaccttga cgctgttgcg gatgtaattc
     6601 gtgccatcga aacgagtcgc ctgcgggaag cgggacgtcg tcgtataggc gtccatcacc
     6661 cagtacagtc gaccgtcggc gataacagca tacggatcct ggtcgtaatc gaggaagggt
     6721 gcgatctttt tcgcacgctc gacgatgttt cgctccagaa ccagccggga gtcaccggtc
     6781 aactggctgc tgaggaaaac attgcggtcg ccgagcgtca acgcggccaa cccccgggtg
     6841 acagggttgc cgagactgac cgagcccgca acctctccct ggaagccttc gacttctgca
     6901 tctccagcct cgacaagtcc cgtcgtctcg gtctggtccg tgaagaggat gatccacctc
     6961 agatcagttt ccccaaagta gacctcaggg cgatcaagtg cgagctcctc gggaccttcc
     7021 atcgggatgc cactgacgat gaagggcggc caaccgtcgc ccgacacctc actcaccggg
     7081 ctcacgacga caccataccc atgtgtgtaa acgagatggg tgttggtcca ggttcggcga
     7141 ttctcggcaa ggccgtccag attgagttcg cgaacgccga ccaacacctg cacgggctca
     7201 ccgtcgatct catagcggtc cacatcgaca tctggaaact cgtaggacgg cacgaacgac
     7261 tgcacttgct ggaagacggg tccgaccacg cgatagtccc aaatacgcac gttatcgagc
     7321 ggcggttcat cagccgagag ctcggagata tcgatctcct cctgccccgt aacgtcgtag
     7381 gcctgcacat cgtttagcgc aaatccagcc ctcgtcatct cgatgttgcg ctctatatag
     7441 ttttcttcac ggctgaactc gttcggctcc accagcaccc gctgcaccgc cattggcagc
     7501 agcggcgtga ccacgacggc gagcaggaac cagctaccga ggagccccgc cagccacttc
     7561 gggttccgga gaataaagcc cgaaagtaac ccgatcgcca ccagcgccga cgccagcgcc
     7621 atgagccagt tcagcggccg cacgatattt acatctgtga aacctggtcc ggtcacaacg
     7681 ccacgggagg aatagacgag ctcgaaggtg ttcagcaggt agcgcatggc aacaaccagc
     7741 agcaccgcgc tgattagccc gctgaggtgg cgaagtgcca cgaacggcac atcaccccat
     7801 gaccggaagc ggatgcccag gcggatgagg taaacaagtg caaccgcgag gatcgtcacg
     7861 atcaacaaag cgagcaaccc tgtttcaagc gccctcagca ccggcaggag gaatacatag
     7921 aatcctgcat cccggccaaa ggtgggatcc tcgatgccaa acgagccgcc atacagggca
     7981 aggagcacgt cttgccagcg ctgggttgcg aggactccgg caacaagcgc gatgatgaga
     8041 ctccccagta cggtgaggcc gcggatgaca ccccgacttg cccggctgac tctcccctcc
     8101 tccgcgagct gcggactccg ggtattccgg agcgcgagcc tcatgttcac gtagaagatg
     8161 agaccggcaa tgaccgcagc ggcaaggaac aacagggcct gggcgttgta gttggtgaca
     8221 atgactgagc ggtatccgac actgccgaac cagagccagt tgatccagaa cggcgccgtc
     8281 acgaacatga ggacgatgag agcgagaacg atcaccgccg ccgtaatcat gcgtctcgtc
     8341 gacgccgaga gggcaaaccg aggacgtctt ggaccgcgcg ctgctgcagt ggacggggat
     8401 ccggcccgcc ctcgtcttcc aaataccacc tggattccat ttcctggcga agttcgcctg
     8461 tcctaaaatc aacgtaccgg atcgtgctac gatcatggtg agcataccgc acggtcgctc
     8521 gtccctgatg gccctatcaa cagaaattct tgccattcca tgcagccttc gacaccgtcc
     8581 tgcgttcctc gccctcgcgc catctccgtg cagagcctcg gcagtggcag ctccggcaac
     8641 gcctttctga tcaccacagg taccgacacg ctgctcatcg attgcggcgt cggtattcgt
     8701 accctcacag catcgctacg caagcgcaat atagctatca acgagatcag cgccatctgc
     8761 atcacccatg agcacagcga ccacatacga acgctgccga aagtgatgca tgagggagcc
     8821 acgttctatt ccacgcgggg caccgcgagt cgaagcgcgc tcccgatgga acggcaccag
     8881 cgcgcgattg aggatgtacc ggtatggatt gccggcgtag cgatttggtc actccccgtc
     8941 gcacatgacg cgatggagcc atgcggcttt atggccgaac ttccagatgg ctcccgggtc
     9001 accatgctga ccgatctcgg gtcctggcag gaatcattga gggagtttgt ccgcgcgagc
     9061 gacctgatca tcctggaggc aaatcacgac gaagagatgc tccggctcgg tccctacccc
     9121 acctacctga agcggcgcgt cgcctccaat actggacatc tctcaaatcg ccattgcggc
     9181 cttgcgctcg gtgaggctct ccgggggtcg acacaccacc cgcagatctg gctggcgcac
     9241 ctctcggagc acaacaatcg cccggatctc gcggaggaga cggtccggtc actgcttgcc
     9301 gagcgtgaac ttgacctcgc cgtcaccgcc cttccccgcc ggtccccaag tgagatatgg
     9361 acgcccggag aaagatccgg gacaccgcag accttcaacc gcatgcgtcc ccagaaggta
     9421 gagcaactgg gattcgatct gttctgacag ctcaagggga gcgtccgaca cccatccccg
     9481 atctatccag atgattgaga agtccactcg acacgccatg tacaccctca ccgctaaggt
     9541 gattccccct gagccaaatg gcctttgggc acatgctgtc caagcggaac gcttggttgc
     9601 tcgttaggaa gctccgcctg tcgtcatgcc gcggattgcg agaccgaaga taaagagcgc
     9661 caccaggcta tagagcagga gtccctgtct gggcgcacga tccctggtat acgtccacag
     9721 gaacggcatg atggccacgg ccgttaaacc atacaggtaa tgtgtcgagt ccgcgggggc
     9781 agtgccaagc gccagaagca caacaccgaa accaacctgt atcacgacca ggacctgtcc
     9841 gatgatcaac gccccagcga cattaccgcc aagcgcacca cccctcgcgt attcaaaaag
     9901 cccccacaat cccacgaaga gaaaaaagag catgacggta gtggcgaggc gattgtgaag
     9961 cagctcaatg acatccatgg acggtattcc gggagagcac aggtgtgccg caagagcgaa
    10021 ggccccctcg ctcactttga tggcattcag tataagtggc aaatcaccac ggtcctgaag
    10081 cttgtcagca ggtgcggatt cgggcccact ttctgccccg atgacgggcg agcctggcct
    10141 ccgcacggtc tcaacgtgat tagacggtgt tgacgtacat acaccattga cagtccgaac
    10201 gcacaaggcg tacaatccga ccgtacagtc tggtgcgtac ataccgatcg ccgaagacat
    10261 acagcgctac tctgttgagc cgtgacgacg cttacgaaag taagcattcg gacctgaaag
    10321 gagtcatcgt ggagtccgag ccccgagact accaggaggt catggacaac gccttgcagc
    10381 ttgtatacag ccatcatcat cgattggtgt cccagcttat gcctgaggca ttccgaccgt
    10441 tgacgctgga tcagcttcgc aatggaccgt tgggccagga ccttcttcgc ctcgcacgtc
    10501 tggcacgtgg ccagattcgt gaacccaagg agcaagtgct ggacgcgatc gattccgtcc
    10561 ttcagctcct cttctggcca gccgcggcgg aagactattc cgtgaaccgc ggattttggg
    10621 acacagacct tggccggatg ctcgctctcg cgaagtaccg tgcctatgag ccaaacgagc
    10681 tcgtgagcat cggcaacgcc gcgcagcagc tcggtgtgac tcgcccgaca gtttaccgtt
    10741 ggatggatga ccggacgctc agctatgtac gcgatgacat gagtggacgt accttcgtgg
    10801 ttcgccggga tatcgacaag ctgaaacgcg tcgcccagga gttcgaggga caaatctaga
    10861 cgaatcgggc cctgggtgtc gtgacagggg aaagggtcac ggcgttttgt gcccgtaaag
    10921 gatgcggatg gagcaaaggg gctggcactt cagtgtgccg gcccttttgc tgcctgggat
    10981 ttggtcgatt ccttcgattt tccagaggtc gccgaactca ccttacgcgg tcgtgagatg
    11041 tactggcgtg gaggaccggg atcaaagatc cctactctca agggcagaac acaaggccgg
    11101 cacaacacct caagcgcact tgggatgata gacctgtcac tccgtgaccg gttgtgagac
    11161 gtgatcgcgc acgatatgcg tcatctcgtt cgtgagactc ataccctacg gtatccctcc
    11221 aggtcatcca ctgcgaacag tcgcccgccc ttgatataga gcttcggcaa ccgtggcgcg
    11281 agacccgtca tgagctcgtg cggaatggtg cccgccatct cagcaagcgt ctccaacgtc
    11341 ggggcgccgg gcgtctcatc agtaccgttt cccacgatca cgaccggttg acgggtttcc
    11401 cctggaagac tgtccggcat ctgcaggatc gtctggtcca tgcagacacg gccgatcacg
    11461 tttgcccgct ctccgctgac ggtcatccag cccttcgatg aaagtgaacg gagatagccg
    11521 tccgcatacc caattggcac caatgccccg cgacttttct ctttggctac ccaggctccg
    11581 ccgtatccga ctcgatcccc aggggccaag gggattaccc gcgtcaccat gccatggatg
    11641 gtgaggatct gcttcatggc ccgctcccct cctggaagcg gaatatgcgg ggctggacgg
    11701 acaccataga gcgcgagccc ggagcgaacc aggttcttgt gatactggga gaactgcacc
    11761 gtggtcgcgc tgttcgcaac gtgatgaaca ggtatctcga tgccagcctc actcagcatc
    11821 ccaacacatc gatcaaagac gttcacttgc tcgtgcgcgg aggtgaggtc cagctcatcg
    11881 gcacaggcga gatgagtcat caatccttct agcttgagct ccgggtgctg catgatggcc
    11941 cgcgccgatt ccacgaccga ctccggcggc acaccgaaac ggcgcatgcc cgtatccacc
    12001 ttgaggtgca ccttgatcgg ctcggttcgc tgatgcatcc gcacgctgcg agcaagacca
    12061 gcggcaaacg gcacatcact catgacaatg gccatgtcca atccgacagc ccgacctcgt
    12121 tccgcggcac ccatcggtcc catcgcaagg atcggcgcgg agatcccagc cttccttagc
    12181 tgggccccct cgtccaccgt tgccacggcg acaccatacg ccccgttctc aaggaccgtc
    12241 tcggcgaccg gcaccgcccc gtggccataa ccgttcgcct tcacgacggc gagaatcgat
    12301 gcctccgggc gcacgtgcgc gcgaagtgtc tttatgttcg tagcgagcgc atccagatcc
    12361 acgacagcaa aggtcggacg gccctgccac gactcaacga tgtcccgaat gcccatcacg
    12421 cgcgattctc cagctccgcg atcacgcgta ccagatcctt tcgggagacg atcccgacat
    12481 atcgattgtc ctggtctacc accggcagcg gatgcacgtc ccgctcgatc atgatggagg
    12541 caacctcatg cagcgttgcg tcgtgccgaa tgctcacgac tggcgatgac atcaacatcg
    12601 ccgcgttgat cgccagagcc cgccggatgt catcctcgta ggggcggcca gcatcagcga
    12661 caaagatggc atcaagaaaa gggacaggcg tgggcgcgtc cacgtcagcc tcgcgggcga
    12721 cgatatcgct ctcggtgatg atgccgatga tttcgtcatt atcgatcacc gccaccccgg
    12781 caacatcgta gtcgaccatc agctttgcga cccgggccac actctcctcc ggcgccacgg
    12841 cgggcacatc cttgcgcatg atctcggcaa caacaggctc cgatgcgtcg ttctgccgtg
    12901 acaccacatg atccttatcc aatgtgctct ccctatcgat gcatgaagtg gtatagcgcg
    12961 taacgcgctc ctcgccttcg ctgctgtgtc ggattaccgg tcgaacgccg tcagcgtgat
    13021 tcattgcccg tgccgagttt cccgaggaca cccgctatct gatcaggcat atcggtggcg
    13081 atgacgccga ggtcgccgaa tcggtactcc agggctcggg ccgccttcgc tcccgcaaaa
    13141 agcgccaaac ctgcggcatc cagcggtgtc aatccctgcg ccagcaacgc accaacagtt
    13201 ccagagagca catcacctgt gccggctgtc gccagagagg tcggcgcatc ctcagcgaca
    13261 aaagtcgcct tcccatccga ggcggcggaa tacgctgatt tcattaggac ggcttgtccc
    13321 catttctccg catactcgcg tgcaacgtgc gaggggtcag caacgatctc ttcagtggat
    13381 agtcctgtca ggcgggccat ctctcccgga tgtggcgtca acaccgcccg cccctgtggc
    13441 atatgggtcc accactcctc ctgcttcgac agccatgtaa gcgcatcggc atcgatcacc
    13501 agcttgatgt gctcatgacc gaagatgggc gaattcgcca cgtcacgggc attggtggtg
    13561 acgaggcctg ggccgccgaa gccgatgtta cttccgatct gggcgttttt cccgccgaac
    13621 ccgaagagtg cgctgagcag atggtcggtc aactcatcgt ccccaagacc cggaccgatc
    13681 accacggact tcgccttctt taggtgcggc tcaaggcgtt cgactgcgcg ccttgccgag
    13741 ccaggagcat ctgtctccgg cagcacgatg tgtgcgacct ctggcatggc cccggcgaga
    13801 gcgacgccca cactccgccc ggaggcgagg tagacaatac cggcaccggc ccgacccgcc
    13861 gccctgctcg ccagataggc ggcgccaatg aaggagggcg aaccggctat cacaatcaca
    13921 ccgcccactt cccatttgtg ggtgccaatc ttccgtcctg gacgtacccg tcgggcaaac
    13981 gtatcgtccg cccacgacgc gttgtgtccc agctcaggtt tcggtatggg gccctcggtt
    14041 gccattcgtc tgctccagct atgtgtgtct atgagttttc agcgacgtgg tccagaacgg
    14101 caccactcgg cagccccgaa ccctcgccgg tccccaccac gaatgccatg gcatcggtcc
    14161 gggagtgcgt cagcgatacg ctgaagctgg tcagaccgag ctggcgagcg cgttctgccg
    14221 ctcgcccgtg caggacaagc acgggtttgc cgcgtcggtt gggtagcact tccatctcgc
    14281 gccagcggat gccgataatt cccgtgccga gcgccttgga cgtcgcttcc tttgcggcaa
    14341 atcgcgcggc caactcgttg acgcgggtcc cataccattc acgctcgcgc tcggtataca
    14401 cgcgcttcag gaaccgatcg ccaaaatcat tcaacgagcg ttgaatgcgg gcgatctcga
    14461 taatgtcggt gccgaccgcc actccacctt cgcgctcggg atcgaattcg ggaacatacc
    14521 acccctcccc ttcgcgctcc tcgagcaccg gaggctgata ttccctccaa cgaggtcgtt
    14581 cgggcatcag cgttgctcct tcttcgggcg ggtgacgcct accggtgagg gattcgccac
    14641 ggggcggtag cgatcggcga acgcgccatg caccttggcg aacgtccccg cggatatcgc
    14701 ggcacggatc tcgtccatct gccgggcgag aaagcgaaga ttatggacgc tcgcaagccg
    14761 tagccccagc acttcatcgg cgcgaaagag gtgatgcagg tatgccgcgg tgaactccgt
    14821 gcaggcaaca caatcacacg tttgatccac cggctcgtga atatgcttcc aggatgcgtt
    14881 acggatcgaa atccggccgt caggagtaaa cagcgcaccg tttcgggcag ctcgggtcgg
    14941 caacacgcaa tcgaacatat cgatccctcg tgcgactccg ttccagaggt cttccggtga
    15001 ccctacaccc atcaggtagc gcggcttgtc ccgcggcaac tcgggcgtgc tgacctccag
    15061 catctccgcc atcaccggtt tcggttcacc gacgctgaga cctccgaccg cacagcccgc
    15121 gacatccatt gacgccaggc ggcgagcgct ctcgcgtcgc aggtcggctt ccattccccc
    15181 ctgctggatg ccgaagagca ccgagcgaga ggcggacccg ttcctgtccg tgtagtgcgt
    15241 aatcgcgcgt tccagccagc gggctgatcg ctcagtagcc gcggcaatcg ccgtatgctc
    15301 ggcgggcagg ccgatcaggt gatcaagctg catcatgatg tcggagccga agcccatctg
    15361 cagttcgacc acacgttccg gcgtcatctc ccatttcgag ccatcgatgt gggaggcgaa
    15421 ccgcaccccc tcctccgtca ccctggcatt gttggcaagg ctgaagatct ggaagccccc
    15481 gctatccgtc aggatgggac ggttccaccg cataaaagta tggagacctc cagcctcctc
    15541 aatgatctcc agaccgggcc ggagcatgag gtgataggta ttggcgagga tgatctgcgc
    15601 accggtttct cgcacctcgg cgggatgaag cgtcttcacg gtgccctggg tgccgaccgg
    15661 cataaagacg ggagtttcga cggtaccccg cgtagttgtg agccgcccgc ggcgggcgag
    15721 cgtttctcga tcctctctca gaagctcgaa cgaaaaccgc ccgctaaaaa cgctgctctg
    15781 gccgcgcagc ccaggagata caggtctgtt tacggaatcg atcatgctcc ctcaggcgct
    15841 gcgctgcgca tagccggcgg cacgtcgacg ctgacgggcc cgcgctatgg cgtcatcgac
    15901 aggagcaggg cccgcagacc cggcgctctc aacaacatca gcagcagtgg aagcagcgat
    15961 ttgccgaggt gtacggtcac tgattactga cggtgtagcg cgacgggccc ggcgtgtacg
    16021 ctctggctcc gatggggcaa atacagagat atccgaatac ggatcttcct tggtagcgac
    16081 aggcgcgacg gctgtctttt taatcgtctc ggatgttttc gcgtcggcct tggtctccgt
    16141 ctttgacggt gtcgttcgcg acgatgaggt gctcttgctg gaggtcgtct tcgtgccgct
    16201 cgagcgtgag ccggtggttc cggatttctt tgctgaagcg ctcgatgagc tcgtggtgcc
    16261 agccgacttc ttcgacgtcg ctgtgccctt ggtacccttg ccgagatccc gctggacgct
    16321 cctggtgacg gaatcgacct gcttttgcat tggcccgaga tcaccggtga gctggtttcc
    16381 catttccctg gcttccgcca cggtcttctc gaactcaccc gtgagctctg aggtcatatt
    16441 tcggaagtcc ttgtagagct ttccggcctg acccatcact tcgggcagct ttcctggccc
    16501 gaagatcagg agcgcggcaa ggccaataag gagcatctcc tgcggtccaa caccaaacac
    16561 agtggaactc cttacctaca tatcacatgt ttccgattcg cccgggacgg ttcgagagcg
    16621 acaagaaaag cgagcccggg aacgtggcac aattgctacc tatagagtat ccggcaggat
    16681 aagcgatatc tgcaacacga aggctcgtcc atgatctcgc gtttcaggtc gattcccgcc
    16741 aggactcgtg cgtggctgac agcagccgac tctgcagacc tagcagacga ggacaagaca
    16801 ccgatgtttg atcgagacca cacgaaggtg gtcattgggc tggggaaccc cggcaggaaa
    16861 tatgccggaa ccaggcacaa tctcggtttt ctcgtcgtgg atgagttagc caaacgaacg
    16921 agcgcgcccg cctcgaagca acgcatgagg gcggagatca cggaaacgcg ccctgacaat
    16981 tcccgcctcg ttctggcgat gccacagacc tatatgaacg acagcgggat ctcggtccgg
    17041 gagatcgtcc gttggtacaa ggctccgctg gaggatgtgc tcatcgtcgt tgacgatctc
    17101 gacctccctt tcggtcagat ccgcattcgc ccgaagggca gcgccggtgg acacaacggc
    17161 ctgaagtcga tctttcacga gctgggcagc caggagatcc cgcggctgcg catcggcatc
    17221 gggcgaggtg gaaatcatac gatcagtcat gtgctatcgc gcttctctcc ggaagagcaa
    17281 cagcaccttc cggagatcat cgagcaggca gcggacgcgg tcgagcactg gatgcgacac
    17341 ggtatgctcg cgacgatgaa cgtgacgaac gggagcagca gtcccctata attccggcgt
    17401 ggacaaacag gccgacgcag caaggagcac cccgcagtga cccccgaaca ggaacaggaa
    17461 gcaacggcta tccttggcgt cgttggcgcc ggcaccatgg gaagtggcat cgcgcaggta
    17521 gcggcccagt ccggctacaa cgttgtgctg atcgacacgg aggaagagat gctggatcgc
    17581 ggtctcgccc gcatcacctc cgacgtcgac agcctggtcg agagggagcg actctcgtcc
    17641 gagaatcgcg acgaggtcct ctctcgcatc gaaggcgcta ccacactcga gcggctggat
    17701 ccctgcgagc tcgtgatcga ggcggttacg gaggatcgcg agatcaaggc cggtgtcttc
    17761 aagcagatct cggaatccgt cgatcctgaa acgatcattg ccagcaacac ctcgtccctc
    17821 tccatcaccg agctggcgca ctgtgtcccg aaccctcaac gggttgttgg gatgcacttc
    17881 ttcaatcccg ctacaagaat gaaactggtt gagatcgtgg ccggacgcgc gacctcagag
    17941 gatacgatcg cggtcgcgat tgcaacggcg atgcagatgg gcaagacgcc ggttgtagta
    18001 ggtgactcgc cggggtttgt tgcgaaccgc ctgatgattc cgatgatcaa tgaggcgatc
    18061 ttcacgctca acgagggcgt agcatcctgc gacgatatcg accagattat gcagctgggt
    18121 gcgggccacc cgatggggcc gctggcgctg gccgacctca tcggtctcga tgtctgtctc
    18181 agcatcatgg aggcgctgca tcacgaattc ggcgatgaca agtaccgtcc ggcgccactg
    18241 ctgcggcaga tggtcgccgg tggccagctt ggcaggaaat caggcagcgg tttttaccag
    18301 tatcaggcgt gaggtagcgc gagatgggta ccggtcagga actcatggca gcgtcagggc
    18361 ggagccgtcc gattcgcgtg ctggttgcaa aacccggcct ggatggacat gaccgcggtg
    18421 ccaaagttat ggcacgcgca tttcgggatg ctggtttcga ggtaatttac actggtctct
    18481 tccaaacgcc gcagatgatt gcaaatgccg ctctccagga agacgttgat gtcgtcggcc
    18541 tgagcatcct ctccggtgct catatgtcac ttttttcacc tatcatggac gcattgcggg
    18601 acgttggccg cgaggatgtg ctcgtcgtgg caggcggaac gatcccggat gaagacattc
    18661 cgacactcaa tgaactgggc gtggcgaaga ttttcggtcc tggtactccc ctccaggacg
    18721 ctgtcgcgta catacgcgag cacgcacctg atcgcgtgga cctgccctag tgacggggac
    18781 tccacaccgg gccagccttt cggaacgtgt gctggcaggg gaccggcgcg cattggcgcg
    18841 tctcctgacc tccatcgaaa acgacgatcc gcttggcgag caagctatgg aaaagctctt
    18901 cccccacacg ggaagcgcgc atctgatcgg catcaccggt cctccaggcg gcggcaagtc
    18961 aaccctcgtc aacgagatga ttcgagccta ccgcgaaatg cccaaaagcg tcgcggtaat
    19021 cgcaatagat cccagcagcc ctctcaccgg tggcgcggct ctcggtgacc gaatccgcat
    19081 gcaggaatgg catgcggacg tcggcgtctt tattcgcagc atggcatctc gccgcttcgg
    19141 aggtggtctt gcaaggaatg cactggccgt cgcccacgcc ttcgatgctg ccgggtttga
    19201 ccagatcatc attgagacag tcggcacagg ccaggacgag gtcaccatcg cgtcccttgc
    19261 gatgacgacg cttctcgttc aggtcccggg gaccggtgat ggtgtccaga cgctgaaagc
    19321 tggtgcgctt gaaatcggcg atgtgctcgt cgtgacgaag ggcgaccgac cggaatcgag
    19381 cgatctagcc cgtgatctgc gaaacctccg ccagctggga atggctgcgg gcagtgagct
    19441 ctcacgttgg gacacgcctg tgataaagac gagcgcggcc cgtcgcgagg gtatcgactc
    19501 gttgatgaac gccatcgccg cccaccggtc ctggctccag gaaaccgggg agatggaatc
    19561 ccgggtccag acgatgatca acacggaaat caccttccgg gtacaggatg ccctgctcca
    19621 ggagatcagg cgtccggtgt ctggagacgg cggcatgcag cggctcgtcg agcgcgtggc
    19681 cgcgcgtgag gttaccccgc accaagccgc cgacgagatg ctcgctcgcc tgcgtcacaa
    19741 cgacacggca ccatagacgg tctttccaga gcgagcggat cgattctccc cacagtcttc
    19801 tgccggaagc cattctccgt cagtcatgtc caggcagccc tgaccccggt gctatacttc
    19861 ccaagttatc gagcattgcg tttttcaacg attcctggag tacataccgc atgtccggac
    19921 attccaaatg gtccacaatc aagcgcgcca agggtgcagc agacgccaag cggggccaac
    19981 tgttcaccaa actggcgcgc gaaatcaccg ttgctgcacg gaccggactt cctgaccctg
    20041 aagccaactc tcgcctgcgc ctcgcgattc agaaggcccg tggcgagaac atgccgaagg
    20101 acaacatcga ccgtgcaatc gcccgcgccg caggcgccga tggcggttcg cagtacgatg
    20161 agatctatta cgagggctac ggcccaggcg gcatcgcact catgatcctc accatgacgg
    20221 acaacagaaa tcgcaccgtc ggagaggttc gcgctgccct cacccgcagt ggcgggtctc
    20281 tgggtgagaa cggcagcgtg agctggatgt tcgaccaggc tggtctaatc accattccgg
    20341 tggacggcca tgacccggac gagatccagc ttgttgcgat cgacgcgggt gccaccgatg
    20401 tgcaggtgga agacacggcg atcgagattt acactgaccc ggccgagctt cacaaaattg
    20461 ccgagcagtt caccgaagca ggctacgaac cccaggccgt ggaactcaca atgaaaccga
    20521 agaccatgct ccagccagac gaagactcgg ctgtcaaggc aatccggctt gtcgagaaac
    20581 tcgaggatct ggacgacgtc cagaccgtct ataccaacct ggacatctcc gacgaggtcc
    20641 tggctcaggt cagctagccc cgcatccacg agaggccggt gatcgatgtc cgtcacgtcc
    20701 ggttcccgat cggaaccgat cacactcggc attgatcccg gcactgctcg cctgggatat
    20761 gggttaatcc ggggcggcat cacccccagc ctgatagacg ccggagtcat cgagacttgg
    20821 cccgaccagg acatgccggg gcgtctcgtg accctctacg aaagcgtccg ggagctaatc
    20881 caggagtttg agccgcagat gctcgccatc gaaaagctct ttttcgcccg gaatgtgacc
    20941 accgccatcg ccgtcggcca ggcgcgtggt gtggtcttgc tggctgcagc ccagtccggc
    21001 attaccgtca gcgaatacag tccctccgag atcaagcatg ccatcgccgg ctatggcaag
    21061 gcggacaaaa accagatgca ggagatggtt cggatgatcc tgcacctgag aaatgtcccg
    21121 caaccggacg atgcggccga tgcccttgcg attgcaatct gccactccca gaccgcatcc
    21181 tacgaggttc gttccctgcg ccatggaaac cgcgaatgat aaagacgcag cggctcgatc
    21241 atgccatggt ggagcgcgag ctcgccgaca cccgctcccg cgccaaggca ctcatcatgg
    21301 caggtgacgt actcgtcaat gggcaaaagg agatgagggc cggtcacaac gtgggaccgg
    21361 cagacacgtt gacgctccgg gcgaagccac gctttgtcag ccggggtggt gagaagctcg
    21421 accacgccct aggtgttttt ggcatggacg tcaacggcat gaccgctgct gatctgggct
    21481 cgagcacagg cggatttacc gactgtttgc ttcagcgcgg tgcctcaaaa gtctatgccg
    21541 tcgatgttgg ctatggcatt cttgatgagc gcatccgcac cgacccccgc gtcatctcca
    21601 tggagcgcac caacgcccgc tatctcaccg aattgccgga gcctgtcgac atcgtcgtca
    21661 tcgacgtttc gttcatctcc ctctccctga tgctccccgt cgctgcccgc attctggagc
    21721 ccgagggtat ctgcgtgcca ttgatcaagc cgcagttcga ggccggcaag gaaaacgtcg
    21781 gcaaacgtgg agtggtgcgc gaccccgctg ttcacgagat ggtgcttcgc aatgcgatcg
    21841 gccatgcagg gaaaaacaac ctcagcattc tcggcctcat cgcctcaccg ttgcgtggcc
    21901 cgaatggaaa tgtggagttc cttgcctcgt tgatcaggga tgcatcagtc gagtcaccgg
    21961 atgtcgaccg tttgatcgcg tctgcaatgg cggaggccaa ggcccttaca gaggaagcaa
    22021 catcgtgacc gatccctttg ctgatcttct tgccgccctg aacggcactg cacccgaacc
    22081 ggtgagtccc taccttcggg tcgatccgtc ccgagccgcg cgcacgggcg tgccggagat
    22141 tgtcttcgcc gagcgaaaga cggtcgacga ggtactggtc gcactcagga gtctcgcgca
    22201 atcaaacggt cgagctcttg ccagtcgcct gacctcagag cagctggatc atctcgggtc
    22261 tttttcagag cccggattca cgctggacat cgacaccgaa gcgcgcatgg cgatcatggc
    22321 ccagcctggc atgcaagacc cgcagattta cggccgtatt gccgtcattg cggccggcac
    22381 ctctgaccga tcggtcgccc gggaggcgac cctcaccgcg cggatgatgg gatgtgaggt
    22441 tatcgagatc cacgatgtgg gggttgcggg gctgcacagg cttgtccagc cactgcggag
    22501 tgcagtggcc tcgggtatcg atgccatcgt ggtcgccgcg ggcatggacg gagcgttgcc
    22561 gagtgttgtc accggtcttg tcgatgtccc ggtcgtcgga cttccggtga gcgttgggta
    22621 tggcgcgggc ggaaatgggg agggagcctt gctcgcaatg ctccagtcgt gcgcacccgg
    22681 gatggcggtg gtcaatatcg ataacggcat aggtgccggt tccatggccg cgctcattgc
    22741 tcgccgaaag acccaatgaa ctcgccaaac cgcttggtac cgaggatatt ctgtatgcag
    22801 atgccttgaa acccggaggg atcacctact agactatgct ctcataagca ttggtgaatc
    22861 tcgtgaagcg gggaggaata tgagtcgttc gaccgttccg tcattcgaac cgcagattgg
    22921 ctgacccgac gcctcgcgcg ttgggttttg ttttgcggcg cgtatgtaac gaacgaacac
    22981 aacggtacgg tggcccacgt ttgggatggc tttcgccgta gcaccaccgg attctgaaaa
    23041 ccaagcgcgc aacgccctgc aggtcaacgg tgactccgag agtgctgcgc tcgcccgaga
    23101 ggtggcaacg cttcaatgga tacgagcctt ataaccaagg ggaaagatca gggatacctg
    23161 ctttccgagg agatcatcgc ggccttcccg aatgccgatg agcaatcgga accactggaa
    23221 gaattttatt ccgcactggt tgctgaaggc atcgatgtac tggaccggca ccccaagcct
    23281 cagaagaccg cccgtgttgt ccgctccaaa accgatgcga gagccggtac cgaagatccc
    23341 cttgctggcg gagttggcga ttctgtacgc ctctatctgc aagagatcgg cgaaaccagc
    23401 ctgctcagca tggaagaaga agtctggctc gccacccgca tggaacgagg caaagccgcc
    23461 gaagcgcgtc tccaggagcg cggccttaac gaggaagacc gcactgatct tgaattggac
    23521 aggaaagatg gagatgcggc acgtgcccat ctcatccagg cgaatctccg cctcgtcgtc
    23581 tcggtggcga agaaatacgt cggtcgtggg ctctccttcc ttgatctgat ccaggaaggc
    23641 aatatcggcc tgatgaaggc caccgacaaa ttcgattacc atcgcggctt caagttcagt
    23701 acctacgcaa cctggtggat tcgtcaggca attacccggg ccatctccga ccagagtcgc
    23761 acgatccgtc ttccggtaca tgtcggtgag acgatcaacc gggtgaaaaa gacatctcac
    23821 cgcttgcagc agatcttcga gcgggaacca acacaggagg agatcgcgag ggcaatgagt
    23881 atcacggacg acaaggtccg gcaggtattc gacgtctcgc gacatcccgt ctcgcttgaa
    23941 gcaccagtcg gaagcgacgg cgatgccttc cttggcgact ttattgagga cgacaccatg
    24001 ccggcgccgc tggagcttgc ctcgcagcaa ctcctaaagg cccaaattgg tagcgccctt
    24061 gaccggctct cggagcgtga gcgccgcatc gtcgtgctgc gttatggcct tgaggatggg
    24121 cgcttccgca cgctcgagga agtcgggcgc gacttcggta tcacgcgaga gcggattcgc
    24181 cagatcgaag caaaggcatt gcgcaagctc cgccacccgg actccagcca acccctgcga
    24241 ggctatctgg actagtcgac acttgggtag cgcgagcacg gcgagactcg ttgcacgagc
    24301 ctccactgca gccactcccc attactgaaa aagaacagcc ccggaccgag gggtcggggg
    24361 ctgttctgct gccacgcctc tccaacagaa tgttattaga gtcgaaaagc aggtgacgaa
    24421 tcggcaccgg cgtgccgttt agctgccacc tcaagtaaaa ggggcagaca atcaggctat
    24481 ctgttcaccg gcgaccgtct caagaccgtg accagacggt atcagggtcc agtggataga
    24541 tggggcgacg aagccgttga tagtcgaaat ttgtcagctt tgggctactg atgcccggcg
    24601 cgtcgacctc gatcaccttc gtcgcgatcg gctcgaaggc cgcacgaaaa tgggcagcac
    24661 tcttgaggac gaggatgcgc cgctgggttg gctcaatccc gaaggcccgg aaatgattca
    24721 ggtcggatgg gtggccccgc agctcagtca gttgaacctc gataccatca tgcccaccaa
    24781 tctcgagcac cacggtccga cccctgctcg caggcgttcc cgcatccatc ggacctgcca
    24841 acatgaatga cccttcgtgg atcaaccgca ccttccctct tacctcgacc gggggaccat
    24901 gcaggccgtc ctgacgacca ccgatagtga gcgcaacgtc acttcctact cccgcatcga
    24961 tacaggcggc cactgcctca gcgtccatga tctgggcgac cgcggcggaa cgggccccgg
    25021 catcgagcaa acccttgagg acgaccgttc catcgctcgc ggtaccgctc gccccggagt
    25081 cggcaacatc tgccagtaca tagacactgc caggtgctgc ctccatggcc tcggcgatcg
    25141 cctcggagac cggcgtagga ttgacggtga agtcggagcg gcgatcccag cagatgtctg
    25201 ccaactcata agcatatgcg cgcgctaaag cgggatcatt atcggtgacg acgatggtcg
    25261 ccactcccgc gaatggcaca tcagcgaacg gaaaaccgcc gaggactgtg gcggcaagga
    25321 cgcccggttg catctctatc tcgcgggcgc ggtcaatcgc cgacttcagg ggtgactgcc
    25381 atgtcgttac catggactgg ttggggacga taagaggcag acgcgtatgt ttcatcgtcg
    25441 accgtgtgcc gtcccggatg atgttgacaa ggagacgcag tgcctgtgca ccagtctctg
    25501 gcatatccgt atgcgggtac tccttgtacc ctaccagtgc gtctgcactc tccaccatgg
    25561 aatcagagag attggtgtgg aggtcaagcg gcgcgacgat gggcacatcc gctcccacga
    25621 ggtcacgtac cgattcgatc agcggtcctt ccgcatcgtc tacatcctcg agcgccatgg
    25681 ctccatggag gacgagcagg acgccgtcaa ctggcagggc tgcccggatt ccatccagta
    25741 tctcgtcgcg caccgtatca aaggcgtggc gactgaccct cccgccaggc atgcactcgg
    25801 cataaacggt cggaaccagg tcaacgccgc tcgcctttgc ctcggagatg acgccaccgt
    25861 agacggttcc ggttccctca agcccgagga tgtcctgacc gcgggcgaag aagccaaatc
    25921 ccggtcggca gaagtcgtcc atcgtcgtgg ccacacgctg aaaggtattc gtctcttgcg
    25981 ccatgccacc ggtcacgatc cgcataatct ccccttactc ccttcgccag atagcgataa
    26041 tcgatatgaa tccgggttcc tgtgttgacg gttgaagcag tacatcggaa gagatgtttt
    26101 tcagtcgcga aggatcccaa aatctataag cggtgacgcc catggaggtg tcgtcggggc
    26161 tggattcatc tcgatgagtg agcactctct cctggcggag gacgtagcac cacctatggc
    26221 gtagcacaac ctacttgtta cgcaatcggg ggtcggtaaa cgactggagc tcatcgccca
    26281 acaggttgat ggccaggatc gccacagcca acgtcacccc cgggaacagc atcgtccaag
    26341 gggcgatctc catgtacgtc tttccatcgc gcagcaagga gccgagcgac ggcatcggcg
    26401 gctgcacacc aagccccagg aaactgagcg ccccttcagc gatcaacgcc ccggagagcg
    26461 tgaaggcgag ctgaaccagg aggggcgtag agacattcgg gagcacgtac cggaacagga
    26521 ttcgcggagg tgttgctccg ataacgcggg cggcaattgt gtactccgcc tctcgaacac
    26581 ttagtgtcgg accacgtgcg acccgagcga agaccgggat ggtggcaatg acgatggtga
    26641 agatgacgct cttaacaccg gggttcagga ccatcgccac caggaggccg agaaggatcg
    26701 ttggaaaggc aaagagaata tcggtgaagc gcatgacgag ctcatccaga actcccccga
    26761 aatatccgga gacgagtcca agcagggttc ctacggaggc agcgagcagc gccgatgcgg
    26821 ctgcaagtgc aagcgatatg cggaggccgt agacgagtcg cgccagcacg cttcgtccca
    26881 gatagtcgcc tcccatcggg aagtccccgg agggagatgc gaacattgca atcgggctaa
    26941 tctcctcagg atcaccagtt ccaataaatg gccccaggat cgcgatgaca atcgcggcga
    27001 cgaggatgaa cgtcgtcaga gtcccgagcg gactcgtcga tatgaagcgg cgtatcgacg
    27061 agcggtggcg ccgctgtcgc gtccccataa gggcaagggg ttcatcatcc gacgccagtc
    27121 tccgcgaggg agtggccgtc ggtactgttt cagtcgacgc caccggcacc ggtttgcgcg
    27181 tcctcacgct gtcgcctgtg gccatcgagc cgtttcctcc acatgctgtc tcacagcgaa
    27241 aaatcggtac ctgctagtac ttgatgcgtg gatcgaggta ggcatatgca atgtccgctg
    27301 cgaggttcgc cagaacgaac atgagcgctc caacgatcac tgtgccctga gctaccggat
    27361 agtcacgatt gagaattcca gtgagcgcat accggcccag accgggcaat gaaaagagct
    27421 cctcgacgat gacagcaccg ccgaggagat aacccatgtt gatgcctacg atggtcaccg
    27481 ttggaatgag tgcggcccgc accacatgat gcaacgcgac atcgcgtgga tgcaggccct
    27541 tcgcccgggc ggtaatcacg tagggctcgc cgagaacctc taccacgctg gagcgaatga
    27601 cacgcatcac gacggatgta gtaatgaggc ccagggtgaa tgtgggcagg accatcgatc
    27661 gaaggtggtc aatgatgccg tcactcggcg atatgtagcc agagataggt aaaccaatgt
    27721 ggcgggtgga gacaacatag atcaagatcg taccgagaac gaaatccggg atggacagtc
    27781 ctacgagcga tacggtcctt gttgtgatat ccgcgaccga gttccgccgg attgcggcga
    27841 tgacgcccgc cgggatgccg atgacaagcg acatcagggt cgccagtaac gtcagctcga
    27901 ttgtgatgag ggatctccgg acgatttccg aagtaaccga tgaatgcgtg cgtaatgact
    27961 cgccgagatc ccctctaaga gcattgccca gccagagcgt atattgcacg aagactggct
    28021 ggtccaggcc atatttctct tcgattgcgg caaccacctc aggctgctga acagcgttgg
    28081 gaccaaggag tgccgccgca aagccacccg gcacaaggcg catggccacg aagacaatgg
    28141 ttgccacgcc gaaaatcgtg atcaaggcca tgacggtccg tttggcaata tatcgtgaca
    28201 tcgtttcctg gctcagcggt gtgtacccgc cgctcgacat tggctggata cgaacgacca
    28261 attgttgagc ggagcccagc cgggctctta cgcgttaagg tgtgactggg gacgcatccg
    28321 acgtccccag ccacgtaaaa agctcagcaa gctgatcaac cttcagttac tgagacaccg
    28381 tcacgtcatc gaggcggtgc cacaatggaa gcccgaagcc ctcgccttcc gcaaaggtga
    28441 ggttactcac caggtcctta cggtaggaga tgaaattgtc gcgcgtgacg agcggctgga
    28501 atccggcaat tgtcgcgatc gcgtcctcca gctgataaag cacctccagg cgctcctccg
    28561 gatcgacggt tgaggccgcc ttggcaatca gttcgtccac ggttgggtca gtgattccaa
    28621 gaacaggagc aaagtcggac gacccgtttt caagcaggat caggtacggg tcgctgtaac
    28681 cggcccacca ggaaatcgcc agctgggcgg taccctcaac ggcgtactcg ttcacaaatg
    28741 tcgagaggtc gcgctggaca atc
//
LOCUS       DASYWM010000007         6373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_638296, whole genome shotgun sequence.
ACCESSION   DASYWM010000007 DASYWM010000000
VERSION     DASYWM010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638296"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..372
                     /locus_tag="VGR29_00265"
     CDS             157..372
                     /locus_tag="VGR29_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072050.1"
                     /translation="MNSVSTRPAARVFRPTQLIAEALIDRKDSDELITRARQLGFSQD
                     LTRTVVAVCCKDKKAHSPVVTGKSSRS"
     gene            734..1024
                     /gene="rpsP"
                     /locus_tag="VGR29_00270"
     CDS             734..1024
                     /gene="rpsP"
                     /locus_tag="VGR29_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV2072051.1"
                     /translation="MIKLRLRRMGAKKRPFYRIVATEHSSPRDGRFIEILGHYDPLTE
                     PATVKVDAERVKYWMSVGAKPSETVEGLLKRAGVIAATSAEPTEQPAAEATA"
     gene            1051..1410
                     /locus_tag="VGR29_00275"
     CDS             1051..1410
                     /locus_tag="VGR29_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV2072052.1"
                     /translation="MSQEDVEEFDDTYNGDETVSEDDIDQVMDEAADAGDNPAEQLRT
                     LVLWLIDQLVDDPGSVTVEAIERGASVQIQVRLPEADLGKLIGRGGRIAKSIRTALMI
                     AGSRHHMRVSLDIEGQE"
     gene            1598..2086
                     /gene="rimM"
                     /locus_tag="VGR29_00280"
     CDS             1598..2086
                     /gene="rimM"
                     /locus_tag="VGR29_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV2072053.1"
                     /translation="MGRIGGPHGVHGEMKLRILTDDPEHLATIRTVYLGERTVPIVLE
                     SIRFVNEGALIKLAGTDAPEDAALLSGLAVKIAGSDARPLAEGEYFLFQLIGLNAFLE
                     DETPVGAVTDLIETGANDVLVIGERPDSSGDLLVPNHPEFVLEISPEKGRIVVRLPVY
                     AN"
     gene            complement(2244..3038)
                     /locus_tag="VGR29_00285"
     CDS             complement(2244..3038)
                     /locus_tag="VGR29_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV2072054.1"
                     /translation="MSDIKSQPIVLVVAMESERVHVDQLIPGWQNVWGSVWPTLRTLY
                     RGVPLILVRSGIGMVAAAAATEYALLTWTPRCLLNFGCAGAHTREILPGDVIIGTGLV
                     SHGRMRISPDEGIVPMDIPFTVAGEPEPRSELRTSPGLVALAQDAARKTVLDLLPAHP
                     GNGAVRVHSGVIASADVWMQDPTWLDATHQRTGSLCEDMEAAAIAQVAMLHDVPFLTI
                     KDISNNEFHATSPFEGTESALPLSEVGKRAAVLTVATIDRIIASSD"
     gene            complement(3162..3533)
                     /locus_tag="VGR29_00290"
     CDS             complement(3162..3533)
                     /locus_tag="VGR29_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEV2072055.1"
                     /translation="MTEHLPASSPGLKSRQVTASAIKVLTTGWRLSFALIIIGLVIAI
                     LRDEPLTEELGTFTHVLDELITGHSNGFLGLGILVMILAPIISSATIAINFFRIGDRR
                     YGLITTAVFVILMVSITLSVF"
     gene            complement(3538..4380)
                     /locus_tag="VGR29_00295"
     CDS             complement(3538..4380)
                     /locus_tag="VGR29_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV2072056.1"
                     /translation="MDLDYSLALGLAAASAAILGSMLGLGGGVFIVPLFTLYLGVDPK
                     IAVGASAIAVVTNSVVGSSNHLRNGFTNIRLSMLLQVSTAIGAIVGAIIAIWADASLL
                     KVVLGIILVYSAFSMIRSRKAAYPNATSDTPDPLALGSIYPDPASGNEVRYVPQRVGI
                     GVGVSTGAGVLSGMLGIGGGVIQVPMMNLVMRMPVKAAAGTSSFMVGMTAVATAAVFY
                     SSGKIDPRVAVPAMLGVFIGSNLGSTLTKRLKTDRLILVFFVILTYLGASMILDAFDI
                     NPFG"
     regulatory      4554..4627
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4815..5804
                     /locus_tag="VGR29_00300"
     CDS             4815..5804
                     /locus_tag="VGR29_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEV2072057.1"
                     /translation="MNRMLMCVLTVIWLVLAVAIPVGAQEATPSASPAAGGGDLASAT
                     AWLIDQQREDGAFAGFSGEPDPGTTVDAITALVAADHAGVNTGTSIPEALAYLEGGDV
                     ALVYGQTGVGQAAKLALALLAAGRDPRDFAMTNPVSIVEAGQNDETGVYGSGLYDHAY
                     AMMALAATGSEVPASALDALAATQAENGGWSFDGSTDPAMVDSNTTSMVVQAMVATGN
                     ADHEVMAPALEFLESTITDDGAAYAPGAEADANSTALVLQAMLATGGETAGLEAALAT
                     FQTDSGAYFYQAADRAENLFSTVQAIPAAAGAVLPILPEGDATPIAIHGLQAA"
     gene            5814..>6373
                     /locus_tag="VGR29_00305"
     CDS             5814..>6373
                     /locus_tag="VGR29_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072058.1"
                     /translation="MKLLTPENSPISRRMHLTLLVMCVLAIATALIPSASGADMNVAG
                     LVVDYGDGRMSYALVPFAEEEINGIELLRRSGLGLVTVGFGGMGDAVCQIENTGCPVD
                     DCRRRLCQTSDPESPFWRYLRQTAPGDWEFLALGASGASVEDGDIDGWSWTGTEASLP
                     SLTMDEVAERAGANATLLAASDAPSA"
BASE COUNT         1310 a         1811 c         2007 g         1245 t
ORIGIN      
        1 gcagggattg ccttatgacg tttatattga taggatagat gcaagaccaa gtcggtttcg
       61 taaagacaat cgtacgaaac cgcatgtacg atcaggggca ccacacctgt atcgcgcatt
      121 gtatttactc ctcgtaatct gaagggaaca ggacacatga atagcgtatc aacgcgcccc
      181 gcagcgcgtg tcttccgacc tacgcagttg atcgccgagg cgctcatcga ccgcaaggat
      241 tcggacgagt tgattacgcg tgctcgtcag ctcggcttct cacaagacct caccaggact
      301 gttgtagctg tctgctgcaa ggacaagaag gcccactccc ctgtggtgac tggcaagtcg
      361 tcccgctcct gactattctc cgcggcgtac acacatccct gtattagaat ctgaagcttt
      421 gacctgttgc atcaggttat gctacatttt ggcttcaagg ccgcctgtgg cggtctcttc
      481 tggcatgcgt cccggtatgg gcttccctac ttcgggcggt gttgagatcg tcggtcacat
      541 acgaccgggg gctatcgatc gtagccttgg tgagcaacag tgcagcttgc ctctcaaccc
      601 cgggcattcg ttgtgtcgca tgttggaggt ccggctcgca ttgagaaacc tcgtccagat
      661 gcaccggtat gaatcaacga gatgattgat tgggcatgat gcctgagcat tctccgccag
      721 ggagtcagtt actgtgatta agcttcgtct tcgccgtatg ggtgcgaaga agcgtccgtt
      781 ctaccgcatc gttgccaccg agcatagctc tccgcgcgat ggccgcttca ttgagatcct
      841 gggtcactac gacccgctga ccgagccagc caccgttaag gtcgatgcgg agcgagtcaa
      901 gtattggatg agcgtgggag ccaagccgtc cgagacggtc gagggcctgc ttaagcgagc
      961 aggcgtgatt gccgctacca gcgccgagcc cacagagcaa ccggccgccg aagctacggc
     1021 ctaatgtcgg tcgacgagcg gaaccagttc gtgtcgcagg aggacgtcga ggaattcgac
     1081 gacacctaca atggcgatga gactgtctcg gaagacgaca tcgatcaggt gatggatgaa
     1141 gccgcggatg ccggagacaa tccagctgag cagcttcgta ccctggtcct ttggctgatt
     1201 gaccagctcg tggacgatcc gggctccgtg acggttgaag cgatcgagcg cggtgcatcc
     1261 gtgcagatcc aggttcggct gccggaggcc gacctcggca agctcatcgg tcggggcggt
     1321 cgaattgcca aatcgatccg gacggctctc atgattgccg gatcccgaca ccatatgcga
     1381 gtgagtctgg acattgaagg acaggaataa cgccccatcc aaatttcggt ccgcagcgaa
     1441 cggggagccg ccaaccggct ccccgtcggt gtctgcgggg cgtcgaccca agcggccctc
     1501 cgccaaccgg cgcaaccggg gtcagcttcc tgcccgggcc cttatcgaga cagcaggtcc
     1561 cgaaccgaac acgccacttg ccgatgtgcg gttgaccgtg gggcggattg ggggcccgca
     1621 cggcgtgcac ggggaaatga agcttcggat tcttacagat gacccggagc atctcgccac
     1681 catccgtact gtgtacctgg gtgaacgcac cgtgccaatc gtccttgaat cgattcgctt
     1741 tgtgaacgaa ggcgcgttga tcaagctcgc gggaaccgac gccccggagg acgcggcctt
     1801 gctctccggt ctggcggtga agatcgcggg ctcagatgcc cggccgctgg cggagggaga
     1861 gtacttcctc ttccagctga tcggactcaa cgcgttcctt gaggatgaga caccggtagg
     1921 ggcggtcacc gacctgatcg agaccggtgc gaacgacgtg ctggtgatcg gtgagcgccc
     1981 ggactcgtcc ggcgacctgt tggtaccgaa ccatcccgaa ttcgtgctgg aaatcagccc
     2041 ggagaagggg cggatcgtcg tccggctccc ggtgtacgcc aactaggcac agaacaatgt
     2101 gtatagcgaa caggcgagta tcttgctcgc ctgtttttgc gcctggtgct gtccctttat
     2161 ggtgcaagag ttcgttcatt agaccggttt gcagcgtatc gcgggattgt ccttgctgga
     2221 ccatccccta tgaggggtcg ggatcaatcg gaagacgcaa tgatgcggtc gatcgtggcg
     2281 acagtgagca cggcggctcg tttgccgacc tcactcaaag ggagcgctga ctcggttccc
     2341 tcgaagggcg aagtggcatg gaactcgttg ttcgagatgt ccttgatggt caggaatggc
     2401 acgtcatgga gcatcgcgac ttgggcgatg gcggctgcct ccatatcctc gcaaagcgaa
     2461 ccggtgcgct gatgagtggc atccagccaa gtagggtcct gcatccagac atcggcagag
     2521 gcaatgaccc ccgagtgcac gcgcacggcg ccatttcctg gatgcgcagg aagtaagtcc
     2581 aggacggttt tcctggccgc gtcctgagcg agtgcgacga gtccaggcga ggtcctcagc
     2641 tcgctgcgcg gctccggctc accagcgacg gtgaaaggga tatccatcgg tacaattccc
     2701 tcatcgggag agatacgcat tcgaccatgt gagacgagac cggtgcctat tatcacatcg
     2761 cccggcagaa tctcccgcgt gtgagcgcca gcgcacccga agttgaggag gcagcgaggg
     2821 gtccacgtta agagtgcata ttcggtcgcc gcagcggctg cgaccatccc gatcccggag
     2881 cgcaccagga taagcggcac ccctcggtag agtgtccgca atgttggcca gacacttccc
     2941 caaacattct gccagcccgg gatgagctgg tcgacgtgca cacgttcgct ctccatggca
     3001 acgacaagaa caatcggctg actcttgatg tcgctcacga caacgtcctc ctctgcaggc
     3061 tagcgaaggt gtcacaggag ctgaaggggg cgcgtcactg acgagcctcg caaacagtga
     3121 aaatgcagga gttgcgttgg cagcacccga ttacctgggg ttcagaagac agatagcgtg
     3181 atcgagacca tgagaatgac gaagacagcc gtcgtgatca ggccataccg gcgatctcca
     3241 atccggaaga agttgatcgc gatcgtcgcc gaggagatga taggcgcaag aatcatgacc
     3301 agaatgccaa gaccgaggaa gccattggag tggccggtaa tcagttcgtc caagacgtga
     3361 gtaaacgttc ccagttcctc agtaagaggc tcgtcgcgca ggattgcgat aaccagaccg
     3421 ataatgatca gggcgaacga gagtcgccat ccggttgtca gcaccttgat agcggatgcg
     3481 gtgacttgcc ttgatttcag gcccggcgaa ctggcaggaa gatgttctgt catcagtcta
     3541 tccaaacggg ttgatatcga acgcatcgag gatcatggag gctccgagat acgtgagaat
     3601 gacaaagaag accagtatca ggcggtcggt cttcagccgc ttggtaagtg tcgagccaag
     3661 gttggacccg ataaacacgc cgagcattgc aggtaccgcg acacggggat cgatctttcc
     3721 actgctgtag aagaccgccg ccgtcgccac cgccgtcatc ccgaccatga aggagctggt
     3781 accagccgcg gccttgaccg gcatcctcat gacgaggttc atcatcggta cctggataac
     3841 gccgccgcca atgccgagca tgccggacag cacaccggca ccggtactga cgccaacacc
     3901 gataccgaca cgctggggga catagcggac ctcatttcca gaagccgggt ccgggtaaat
     3961 ggatccaagc gccaatggat cgggggtatc ggatgttgcg ttggggtagg cagccttgcg
     4021 gctgcggatc atgctgaagg cggagtaaac aaggattatc ccaagcacca ctttcagcag
     4081 ggaggcgtcg gcccagatcg cgatgatggc gccgacgatg gcgccgattg cggtggagac
     4141 ttgaagcagc atcgacaggc gaatgttggt gaagccgttg cgaagatgat tggatgagcc
     4201 gacgacggaa ttggtcacca ccgctattgc actggcaccg acggcaatct tggggtcgac
     4261 gccaaggtag agcgtaaaga gcggcacgat gaagacgccg ccacccagac cgagcatgct
     4321 acccaggatc gcggctgacg ccgcggcgag acccagggcc agcgagtaat ccagatccac
     4381 gaacgcttct ccccctctga tgaacggtgc ggcattgtca cgtgcccgtt cgcaccattc
     4441 cttataccac catccagggt tgcgtcctca gtctggagta tctggagtag aatggtggaa
     4501 aggcgcctcg gtgcctgcac attgacaatc agctcatcgc gttccgaaaa caggggaagc
     4561 cggtgtaaat ccggcgctgt cgcgcaactg taacgatcga ttcctgatcg atcaaagcca
     4621 ggttaccgct tcggagtgcg tctgagaccc ttcgcgattt caagggtgca ggacaaacgt
     4681 gtctatcacg gcgggtcatc ccccgtggag atactgccgg ggacccgata gggacatccc
     4741 ggtttgtcag gcccgctcgt gaagagcggg cttttttgtt gtaatcgaag caatgagagg
     4801 gacaggaaca acctatgaat agaatgctca tgtgcgtcct aacggtgatt tggctggttc
     4861 tcgcggtcgc gatacccgta ggtgctcagg aggccacgcc gtcagcatcg ccagcggcgg
     4921 gagggggcga cctcgcctcc gcaacggcat ggttgattga tcagcagagg gaagacggcg
     4981 cctttgccgg cttctctggc gagccggacc ccggcacgac ggtggatgcc attaccgccc
     5041 tggtcgcggc ggatcatgca ggtgttaata ccggtacctc gatccccgag gccctcgcgt
     5101 atcttgaggg cggggatgtc gcgttggtgt acgggcagac cggggttggg caggccgcga
     5161 agctggcgct ggctctgctc gcggctggcc gggacccacg ggattttgcg atgactaacc
     5221 cggtcagtat tgtggaggct ggccagaacg acgagaccgg cgtctatggt agtggtcttt
     5281 atgatcacgc ctacgcgatg atggcgttgg ccgcgaccgg atccgaagtg ccggccagcg
     5341 cgctcgatgc ccttgccgcg acacaagccg agaatggtgg atggtcgttt gacggttcaa
     5401 ccgatcccgc gatggtggac agcaatacaa cctcgatggt ggtccaggcg atggttgcca
     5461 ctggcaatgc tgatcatgag gtgatggctc cagcattaga gttccttgaa agcaccatca
     5521 ccgacgatgg tgccgcctat gcgccgggtg cggaagcgga tgcaaattca acggcgctcg
     5581 tgctgcaggc gatgcttgcg accggcggcg agacggccgg gctggaagca gccctggcga
     5641 ccttccagac agattccggt gcctacttct accaggctgc tgaccgggcc gagaacctct
     5701 tctcgaccgt acaggcgatc cccgcggcgg caggcgcggt cctgccgatc ttgcccgagg
     5761 gcgacgccac gccaattgcc attcacgggc tccaggcggc gtaaggcgcc cgtatgaagc
     5821 tgcttactcc agagaactcg ccaatatcgc gccggatgca cctgaccttg ctggtgatgt
     5881 gcgtccttgc cattgcgact gcattgatcc cgtcggcttc cggggcggac atgaacgtcg
     5941 ccggtttggt ggtcgattac ggtgacgggc ggatgtcgta tgcactggtg ccctttgcgg
     6001 aagaggagat caacgggatc gagcttttgc ggagaagcgg gctcggtctc gtgacggttg
     6061 gcttcggcgg gatgggtgat gctgtctgtc agatcgaaaa taccggctgt cccgtggatg
     6121 actgtcggcg gcggctgtgc cagacctccg acccggagtc acccttttgg cgctatttgc
     6181 gacaaacagc tcccggcgac tgggagttcc tggctctggg cgcgagtgga gcaagcgtag
     6241 aggatggcga catcgatgga tggagctgga cgggaaccga ggcctccctg ccgtcgctga
     6301 cgatggatga ggttgcagaa cgggccggtg ccaatgccac cctccttgct gcctccgatg
     6361 ctccgtccgc gat
//
LOCUS       DASYWM010000008         3689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_319407, whole genome shotgun sequence.
ACCESSION   DASYWM010000008 DASYWM010000000
VERSION     DASYWM010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319407"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VGR29_00310"
     CDS             complement(<1..637)
                     /locus_tag="VGR29_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072059.1"
                     /translation="MSSHVEEVAETASGTPPMTQAAEQPVPHSPSTSRVAATRSERIA
                     CAIRTSSVPQRFLALLLVIFLFKGIIVSLVFPPYTGHDEVTHYAYLKIVAEEGRVPLI
                     PDPVVFDREYSRNEEYDDWDMIPDEFYQYSEYVTSDWWSGYAEPVRAVTYQGMYLPSG
                     WVYTGNHPPLFYLLLTPLFWLLADQTIETQLYVFRLLTIPFGMLTVLFAYLT"
     gene            833..1264
                     /locus_tag="VGR29_00315"
     CDS             833..1264
                     /locus_tag="VGR29_00315"
                     /inference="COORDINATES: protein motif:HMM:NF039673.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6157 family protein"
                     /protein_id="HEV2072060.1"
                     /translation="MGVNYVNTLIAVADDCPVTDAVVPPRNDAKPTIAAEQYRLISGH
                     PYGLTSEEVIFQIYADKQANPEDERPAAREEYFSVGRARMRTSPLAKTFGWGIHSDAD
                     GRLALIGMQTEEYRRLESGANGTTVVKAMRSSKKQIVGSRT"
     gene            1369..2341
                     /gene="corA"
                     /locus_tag="VGR29_00320"
                     /pseudo
     CDS             1369..2341
                     /gene="corA"
                     /locus_tag="VGR29_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
     gene            complement(2460..>3689)
                     /locus_tag="VGR29_00325"
     CDS             complement(2460..>3689)
                     /locus_tag="VGR29_00325"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2072061.1"
                     /translation="RYVDAAFRARSARIIPVSARARFIRMGIPADVVDDTLGRIRRAN
                     HWSSQWVETAQRFLGDFRRQTSAANVREAAQARQLAALCYHAAQIFEPFDPMTVRKCR
                     SASASLFTQALPQLHPNVRHLWIPWRSKSLPVYFELPERVSEPVGLVVILNGASMSKE
                     ETFAWRGQLIDHGYAVILMDSPGTGEATSVGSIGPDQNDILDGVFEMFRNQPMIDLSR
                     VVALGASLGGNQAIRAAAHDRRIMAAVAVTPPYDPARWIHRASPLLQAELGLIVDDKL
                     VPEARDMVAGFSLADVSLASRQPVLIFGGGRDVIVPPTEAQLLAKRVGGRATLVWYPT
                     GGHCLYELVDQWMFEAAVWIDAVAHVQRSGGLQDNPTKVAAVAAEALELAEYAPKSGP
                     NDWETGEELGEYARLLT"
BASE COUNT          813 a         1099 c         1042 g          735 t
ORIGIN      
        1 ccgtcaggta ggcgaacagc actgtcagca tgccgaaggg aatggtgagc agccggaaca
       61 catagagttg ggtctcgatc gtctggtcgg ccagcagcca gaagagcggg gtcagcaaca
      121 gatagaagag cggcggatga ttccccgtat acacccaccc ggatgggaga tacatgccct
      181 gatacgtcac agcccgcacg ggctcggcgt atccgctcca ccagtcagag gtgacgtact
      241 cggagtattg gtagaactcg tccgggatca tgtcccagtc gtcatactcc tcattgcggg
      301 agtattcacg atcgaacacc actgggtcgg ggatcagtgg cacccttccc tcctccgcca
      361 cgatcttcag atacgcgtaa tgcgtcacct catcgtgccc ggtatacgga gggaaaacca
      421 gcgagacgat aatgcccttg aagaggaaga tgaccaacag aagcgcaaga aaacgctgcg
      481 gaaccgagga cgtacggatt gcacatgcga tgcgctcgga acgggtagca gcaacacggc
      541 tcgtggacgg cgaatgggga acaggttgtt ccgccgcttg cgtcatgggc ggagtgccgg
      601 atgcggtctc agcaacctct tccacgtgcg aagacacgaa ttgcggctcc tcgtttactg
      661 gatcatcgac agacatcaag gaggccctga gcctgtcccc ggcctcgacg attcagtgta
      721 cacgcatgca gcggcaccga ggccatcgtc acagcagcac gtcagtcacc ggagcgtgat
      781 gctacatttc gccctctttg gaggctcgtc aaggtacagg gaagtgaaat ccatgggcgt
      841 aaactatgtc aacacgctga tcgccgtcgc agacgactgt cctgtcaccg acgcagtggt
      901 tccaccccgg aacgatgcca agccgactat cgcggcggaa cagtaccggc tcatctccgg
      961 gcatccctac gggctcacgt cggaagaggt cattttccag atatacgctg acaaacaagc
     1021 caacccggaa gacgaacgtc ccgccgcgcg ggaggaatat ttctctgtcg gccgagcgcg
     1081 catgcgaaca tcaccgctgg caaaaacgtt cgggtgggga atccatagcg atgctgatgg
     1141 ccggctcgcc ttgatcggca tgcagaccga ggagtaccgg cgactggaat cgggagcgaa
     1201 cgggaccacg gtcgtcaaag cgatgcgttc gagcaagaaa cagatcgtgg gaagccgtac
     1261 ataacacggc gtacaaccac cgctcccgtg tcctgtcttt tcattccgat ggtcttcata
     1321 cttccaacaa ggtgatcacg gattgcaata agcggggtga cacgaagcat ggtatcggtt
     1381 cggctctgtt tcgacgagca tggcacgtat tccaacccaa agggcgagga tgagattcgc
     1441 aacattctgg cgcaccctgg cgctctcgtc tggcttgacg tagaccgtga tcacctaggt
     1501 cacctcaagc cattcgatga cctcatccat ctccatccac ttgcggttga ggacgctgag
     1561 tcaccacatc aacgaccaat cctgaaccga tacgacggca ctattttcct ggttctgtat
     1621 gagctcacca tggcggaggg cgacaatacc attcggggct tccccatcag cttttttgtc
     1681 gggcagaact atgtcgtcac cgcgggacat tgagcgatca acgctcgacg acgtagcaaa
     1741 acggtggcaa gaattcggtc agcataccga gactcacact ccgggctttt tactttttgc
     1801 gattatcgac gcactggtcg acaactactt tccagttgtc gaaacactcg gaaaccgcat
     1861 cgaagccctg gaagagcgac ttttgcaatc ggatgagcgc ctggagcaga aggagcttca
     1921 cgcggttcga aaacaacttt tcgaggttcg acgtgtgctt gctccgggac gtgaagtgct
     1981 caacgagctc atccgccgcg acacgccact gatcgatgag aaatcaatca actacttcca
     2041 cgatgtctac gatcatgtcc tgcgggtcct ggattggatg gatgcatatc gggagatggc
     2101 aggaacactc ttcgaaatgc agctcaccat gtcgtcccat cggcttgatc aatcagttcg
     2161 cacactgaca gttgcatcca ttatcttgat gagtgctagc ctgattgcag gcatctatgg
     2221 gatgaatttc gacaatatgc cggaactggg gtggcggctt ggatatccca ttgcccttgg
     2281 tatgatgctg ctctccgcta acctgctttt tgtcttcttc cggcatcgtc gctggcttta
     2341 aaccaggccc tatggaatga gctcccgcca tgacggcgtc catccactgt tgcgtacgga
     2401 tctcattacg cgaccaaatt atccatgact ttagatcgag tatctggaca tgtggtctgt
     2461 caagtgagga gccgtgcgta ttcgcccaac tcctcaccgg tttcccaatc attgggcccg
     2521 gatttcggtg cgtactccgc gagctccaga gcctcagcag ccacggccgc taccttcgta
     2581 gggttgtcct gcagtccccc gctgcgttgc acatgggcga ctgcgtcgat ccacaccgcc
     2641 gcctcaaaca tccattgatc aactaattca taaagacaat ggccacccgt gggataccat
     2701 accagggttg cccgtccccc taccctcttc gccagcaact gcgcctccgt gggcggcacg
     2761 atgacatccc ggccaccacc gaagatcagc actggctgcc ggctggccag cgatacatcg
     2821 gcgagggaaa agccggcaac catgtcccgc gcctcgggga cgagcttgtc atccacgatc
     2881 aaccccagtt cagcctgcag gagggggctg gcgcgatgaa tccagcgagc cggatcgtat
     2941 ggcggtgtca ccgcgacggc ggccatgatg cggcgatcgt gagccgccgc tcgtatggcc
     3001 tggttgccac ccaaactggc gcccagcgcc accacccggc tgaggtcgat catcggctgg
     3061 ttcctgaaca tctcgaagac gccatccagg atatcgttct ggtcaggtcc gatactcccg
     3121 acactcgttg cctctccggt acctgggcta tccattagga tgacggcata gccatgatcg
     3181 atcaactggc cacgccaggc gaaagtctct tccttggaca tgctggcgcc gttaaggatg
     3241 accaccagac ccaccggttc cgaaacccgt tcgggtagtt cgaagtacac tggaagcgac
     3301 tttgaccgcc aggggatcca gagatgacgc acgttgggat ggagctgagg cagggcctgg
     3361 gtaaagagcg aggccgacgc cgaccgacac ttccggacag tcatgggatc gaacggctcg
     3421 aagatttggg ccgcgtggta gcagagcgcg gcaagctgtc tcgcctgggc cgcctcacgg
     3481 acgttggctg cagaggtctg cctccgaaag tccccaagga accgctgggc ggtttcgacc
     3541 cactggctac tccagtggtt ggcccgccgg attcgtccca gcgtatcatc gaccacatct
     3601 gccgggatgc ccatccggat aaaccgggcc cgtgcggaga ccgggatgat acgggcgctc
     3661 cttgccctga aggcagcatc gacgtagcg
//
LOCUS       DASYWM010000009        13333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_563301, whole genome shotgun sequence.
ACCESSION   DASYWM010000009 DASYWM010000000
VERSION     DASYWM010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563301"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VGR29_00330"
     CDS             <1..711
                     /locus_tag="VGR29_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2072062.1"
                     /translation="VVLDISFRLAPGEVLGLIGRTGSGKTTLTRLLFRLHDPTAGTIR
                     IGDMNLREYRLSDLRAEIGIVTQDIQLFRASVRDNLTLFDSSISDEQIRSALEGLGLA
                     PWLSSLPDGLDSLLASGGAGLSAGEAQLLAFARVFLRHPRIVVLDEASSRLDPGTERR
                     LERAVDRLLEGRTGIIIAHRLATVQRADSIIVLEDGRIVEYGRREDLAADPTSRFARM
                     LRTGHDLLSEEEPAEARR"
     gene            708..2504
                     /locus_tag="VGR29_00335"
     CDS             708..2504
                     /locus_tag="VGR29_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2072063.1"
                     /translation="MRPWRYVLRMAAFRPWLFIASGLSASTLFYLVPLLPGLVVREFF
                     DAITVETTGPSTIWWLLALLAGIGALNMSGLIAAAYFETTLHLYVDALLRKNMLRRVL
                     DHPGARAVPVSPGEAVSRFRDDVTVASNFLSWTLDPVGQVLVLVIGISFLISIDPVLT
                     IGVFLPLLAVITVSRLAGKRIEHYRRANQESIGAVTGLLGEMFGAVQAVKVAGAEERV
                     VDYFRTINEVRRKATLNDLVFNEILRSLSTNMANIGTGIILLFSAQSIRSGELSVGDF
                     ALFVSYLGWLSQVVTMFGFYLNQYRQFGVSVSRMGELLKGVPAENLVEHGPVYLRGDL
                     PGLPGLQTTGQERLEVLEARGLTAHYPETGCGVTDIDLRIERGTMTVIVGEVGAGKTT
                     LLRALLGLIPHDAGEIRWNGRIVEDPSRFFVPPRTAYTPQTPRLFSESLRQNIMLGLP
                     EHEVDLPGALRSAVMEQDLLALEEGLDTVVGARGVKLSGGQLQRSATARMFVRPAQLY
                     VFDDLSSALDVETERILWDRFFERPEATSLVVSHRHAALRRADQIIVLDGGRIIARGT
                     LRELLATSPAMRHLWHGEPGKPEAPAESGAGM"
     gene            2572..3942
                     /locus_tag="VGR29_00340"
     CDS             2572..3942
                     /locus_tag="VGR29_00340"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEV2072064.1"
                     /translation="MAHSHRSDTINRHAPSRPDTITSHAQPLSGEAGDYDRLLEEIGD
                     ARLVLIGEASHGTEEFYRARATITRRLIEERGFGAVAAEADWPDAFRINRYVRNTSDD
                     RTPLDALDDFGRFPHWMWRNTVVHDFVGWLRDYNDAHPNHKAGFYGLDLYSLNTSVRA
                     VIEYLDKVDPAAADRARERYSCFEHFGQDSQAYGMATSLGTAEPCEDDVVAQLVEFHV
                     RAAELAMRDGQVAEDEFFSAQQNARLAKNAEAYYRSMFRGRVSSWNLRDTHMAETLDA
                     LIDHLTQRNGAPAKIVVWAHNSHLGDARATEMGDAGELNLGQLARERHPNEVFLIGQT
                     THEGTVTAASDWDQPAERKRVRPGLPGSYEALFHEFPYGAFLLSMRDAGGMLRTDRLE
                     RAIGVIYRPETERLSHYFGARLADQFDAVIHWDSTTALTPLEPTEQWDRGEAPETYPS
                     GDEPLS"
     gene            3972..4643
                     /locus_tag="VGR29_00345"
     CDS             3972..4643
                     /locus_tag="VGR29_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEV2072065.1"
                     /translation="MRESPQDITIPIRSASLYGMLALPPGATGIVVFAHGSGSSRFSP
                     RNHEVAAALQQAGFATLLFDLLTESEEAVDRRTRQHRFDIPLLGERLIAATDWLSDQA
                     ATGEMNIGYFGASTGAAAALIAAADRPNVHAVVSRGGRPDLAGEALGRVRSPTLLIVG
                     ELDTAVIEMNTRAKAKLGGPSEMVIVPGATHLFEEPGTMEQVTKHAEGWFQRYLASGE
                     QMASE"
     gene            5026..5784
                     /locus_tag="VGR29_00350"
     CDS             5026..5784
                     /locus_tag="VGR29_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEV2072066.1"
                     /translation="MLDPSHQQRAYRLRFEWGMAGAETVARDSDIAIVVDVLSFTTTL
                     SVALDAGTVVLPYGWNDESAALYARDHDAVLAAGRTNARPGDITLSARSIQDASLISR
                     LVLPSPNGSAISHHLASTASTVLASSLRNASAVACWINDNHDAESTTIAVIAAGEQWL
                     GGTLRPAIEDLWGAGAVLSHLHRSGWTDLSPEAEVARHSYEQAAGDILGQLRACASGR
                     ELIGKGFPDDVVIAAELDQSRSVPILRERQFILA"
     gene            5933..6991
                     /locus_tag="VGR29_00355"
     CDS             5933..6991
                     /locus_tag="VGR29_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2072067.1"
                     /translation="MTASPIVLSEADPAIVVDRLRKTYVVPEREGGLKAAVGSLFHRK
                     TKEVEAVREISFSIAPGEIVGFLGPNGAGKTTTLKMLSGLLHPTSGTAGVLGHTPWER
                     DRAYLGQMALVMGQRNQLTWDIPVVDSYQLNKAIFRISDDEFRRRLDELTDLLDLGDL
                     IRKPVRNLSLGERMKCEIAGSLLHYPRVLFLDEPTIGLDVAMQRRIRSFVAEYNGRTG
                     ASVMLTSHYMADVEALCERVIVIHHGTLLYDGGLPGLVRAFSPHKTIIVELEEGAEIS
                     RDHVTTLLPGGGEVVEQTPVGLTLRVPKAETARVAAQVLSALPVFDLTIEDPPIEEVI
                     EAVFASPTSDHHSEPTLV"
     gene            6988..7857
                     /locus_tag="VGR29_00360"
     CDS             6988..7857
                     /locus_tag="VGR29_00360"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2072068.1"
                     /translation="MSRPQIPESSASTGPDFDRLSLKVLWAYYSGKLKIDIANNLAYR
                     GAVAIWVLSTVVQPLVSLVVWRTVAGSEGGSAGGFTANEYTSYFVIVMLVSHMTFIWH
                     MWEFEWRIRTGFFSPILLRPIHPIHHDVSENLSYKVVGLIGVIPAAIILAVVFDADFS
                     GTSVIDILAFFPALILAMALRFLVEWTLALAAFWMTKVSALNNLFDVFFLFLGGTFAP
                     LSVMPGWIQAVSYVSPFRWAIAFPVEVALGNRSGLEILTGYVIQLVWITLSVIVLRLM
                     WTRATRRYSAVGA"
     gene            7862..8608
                     /locus_tag="VGR29_00365"
     CDS             7862..8608
                     /locus_tag="VGR29_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV2072069.1"
                     /translation="MPASDRPHEERLAGGFINEVVRVGDTVRRSGSLNTEFVAPLLQH
                     LDECGWSGAPRFLGIDEHGREILSFIEGVVPVDEDIPPSFSTDETLTALARMVREFHD
                     LTAGTPLAGTEEVVCHNDLSPKNTVYRDDAGIMMPVALIDWDLAAPRGRIYDVGHICW
                     KYLNLGPSVTDLDECARKIHLICDAYGIEDLSGVIDAIIWWQEASIWGIETGAKAGDK
                     GMMRLQSAGVLLEIRASVTWVQKHRTALEQ"
     gene            8664..9452
                     /locus_tag="VGR29_00370"
     CDS             8664..9452
                     /locus_tag="VGR29_00370"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2072070.1"
                     /translation="MHALTVLKTYLQLGLLNVMQYRADFFLQVLGTIIGFATSLIGIL
                     VIFNRTTDLKGWGRDDLIVLVGIQILVAGLINLVIRPSMQQLMEGIRLGTLDFMLTKP
                     ADSQLLASIQMVNPAGIQDAVFGAGVILVGLSLLDAAISPLAVVLFLLVLIAGLIIIY
                     SFMLILSTCAFWFVKLDNVLVIFNTMFGNAGQWPITIYPGWLRASLTFLVPVAFAVTI
                     PAQSLTGRLDGWTVVGTFALAFAFFVASRLFWRFALRHYTGASA"
     gene            9528..10505
                     /locus_tag="VGR29_00375"
     CDS             9528..10505
                     /locus_tag="VGR29_00375"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV2072071.1"
                     /translation="MQRGRPEQFVSSVTKPDDFNAFWDQVQAELDDISLEPAMRHVPE
                     RSTAEVDVYEIHYTSLHSLRIAGWYCTPKPDYPVSPYPGLAILPGYVSEPTLPKSWAK
                     LGYAAVGVAPRGKLRSNGQYNPGYPGLLVDRIVDEQTYGYRGFYADALRAIDFMFTRP
                     EIDPSRIGVHGGSQGGALSLVTAALRPDVVRCGALAAPYLTGFMDAAALTHSYPYEEM
                     NEYFRTYPERIDQVRKVVSYFDCLNFAPKITAPLLIYVGLADDICPPETAFNLRERLT
                     CDVTFRAHAGCGHEAGVYWELPKVEAFLADHLRPAAPARLMTDGLEEVS"
     gene            10502..11416
                     /locus_tag="VGR29_00380"
     CDS             10502..11416
                     /locus_tag="VGR29_00380"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV2072072.1"
                     /translation="MSADLAQEIDQWWERLDDDLCPLEATPEFEHDALRSHDLFDTYR
                     VRMTSLDRYIIAAWLSVPKGDGPFPAAMYTPTYMSVVNPAPYEFRIRAITMNVISRGQ
                     RGADRPYAAAFPGHLTVGIDDPDRYIFRGVVADTIRAWEVLRNHPQVDTDRMAMIGTD
                     LALLVNARRADAKAVHVTGSFWYRMKEIAAGTEAYPFEEINDHLRMWPNQEEAVARTL
                     AHFDPQYQAERITARVLLNRDQASPTGNDAWFAPLASRLATGPTFLDMTHRGQTDYDA
                     MDAWLSNELGMPAYPRSWTPQDLGAWSV"
     gene            11413..12546
                     /locus_tag="VGR29_00385"
     CDS             11413..12546
                     /locus_tag="VGR29_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV2072073.1"
                     /translation="MKPEGMPQDWLRELGLRPVINAAATLTSLGGSIMPPEVVEAMAV
                     GARHFVDYRDLQASVGARIAALTNNEAAYVSSGAAAGITLSIAACMAGKDPDLIRSFP
                     GPDAFQRNEVLVYRSQRNGYDYAASMTGAKMIEVASEGDPLAARINEHTAAVLWFAGT
                     RLRSDTPPIEEIIAIAHEVNVPVIVDAAAQIPPASNLWHFTRELGADVVIFSGGKGLR
                     GPQATGLVMGRAALIEAVAANASPHYSIGRPMKVGKEELFGILAAVEWTLAQDEGAVI
                     YAYEETVKCWIDGLEGLPGVEISRGFPSEAGQPHARAIVRVLPSARRDRDAVHDALWD
                     MNPRIAVLKEGDDVLALNPQTLEPGEAEIVLDALRSVLELPPG"
     gene            complement(12597..>13333)
                     /locus_tag="VGR29_00390"
     CDS             complement(12597..>13333)
                     /locus_tag="VGR29_00390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2072074.1"
                     /translation="IPLACSGFFAVCGILLAFGLDEPARERSHSGRKKGIRAHVHDVH
                     ALLRTRHAVALMLAVGAVFWGSLTVAELYVQAVFSDRGLSNGQIGLLVGSMFLAIAAG
                     TTVGRQLRGSFAWQWPVLAVTTGAGVVLIGLESVVLAITAFVLAQFASGVVETRMSAW
                     YNAQLPSAQRATVLSIESWLFSCLMIVFFPLGGWLAQRSGWSVLYLICGLTGVLTSLA
                     VIVLRKHPETADEPDLLEPVLESSPT"
BASE COUNT         2445 a         4229 c         4110 g         2549 t
ORIGIN      
        1 gtggtactcg atatcagctt ccgcctcgct cccggcgaag ttctcggcct gatcgggcgc
       61 accggtagcg gcaagacaac gctgacccgg ttgctcttcc ggctgcatga cccgacggcc
      121 ggcaccatcc gaatcgggga catgaacctc cgggagtatc gcttgtccga tctccgcgcg
      181 gagatcggca tcgtcacgca ggacatccag ctcttccggg ccagcgtacg ggataacctg
      241 accctgttcg attcctcaat cagcgacgag cagatccgat ccgcgctgga gggactggga
      301 ctggcaccct ggctgtcatc ccttcccgat gggttggact cgctccttgc atcagggggg
      361 gcgggactct ccgccggtga ggcccagctc cttgcctttg cccgcgtctt cctgcgtcat
      421 ccccgaatcg tcgtgctgga tgaagcatca tcccgcctcg accccggcac ggagcgtcgc
      481 ctggagcgag ccgttgaccg attactggag gggcgaacag ggatcatcat cgcccaccgg
      541 cttgcgaccg tgcagcgggc agattccatc attgtcctgg aggatgggcg gatcgtggag
      601 tacggccgac gggaggatct tgccgccgac ccgacatcgc ggttcgctcg catgctgcga
      661 accgggcacg acctgctgag cgaggaagaa ccagcggagg cgcgacgatg aggccctggc
      721 gctatgtcct tcgtatggcc gcgttcaggc cgtggctctt cattgccagt gggctctccg
      781 ccagcacgct cttctatctc gtgcccttgc tgcctggtct tgtcgtccgc gagttcttcg
      841 atgcaatcac cgtggagaca accgggccct cgactatctg gtggctcctg gccctccttg
      901 caggtatcgg cgccctgaac atgagcgggc tgattgccgc ggcctacttc gagacaacct
      961 tgcatctgta cgttgatgcc ttgctgcgga agaacatgct gcggcgcgtc ctcgatcacc
     1021 cgggggcacg ggcggttccc gtgtcgccag gcgaagctgt cagccggttc cgggacgatg
     1081 tcacggtcgc cagcaacttc ctgagctgga cgctggatcc cgttggtcag gtgctggtgc
     1141 tggtgatcgg gatcagcttc ctgatcagta tcgatccggt gctcaccatc ggcgtcttcc
     1201 tgccattgct ggcggtgatc acagtctccc ggcttgcagg caagcggatc gagcactacc
     1261 ggagggccaa tcaggaatcg attggtgctg tcacgggcct ccttggtgag atgttcggtg
     1321 cggtgcaggc ggtgaaggtt gcgggggcgg aagaacgtgt tgtcgattac ttccgaacga
     1381 tcaacgaagt gcggcgcaag gccacgctca acgacctcgt cttcaacgag atcctgcgct
     1441 cgttgtcgac caatatggcg aacatcggta ccggcatcat ccttctcttc tccgcccagt
     1501 cgattcgctc tggcgagctc agcgtgggag atttcgcgct gttcgtctcc tatcttggct
     1561 ggctctcgca agtcgtgacc atgttcggct tttacctgaa ccagtaccgc cagttcggtg
     1621 tctccgtctc ccggatggga gagctgctga agggagtacc ggcggagaac ctggtggagc
     1681 atggaccggt ctacctgcgc ggcgatctcc ctggccttcc cggtctccag acgaccggcc
     1741 aggaacgcct ggaggtgctg gaagcgcgag ggttgaccgc acactatccc gaaaccggct
     1801 gcggtgtgac ggatatcgac ctccggatcg agcgcggcac gatgacggtg atcgtcggtg
     1861 aggtaggcgc tggtaagaca acacttctgc gcgcgttgct cggcctgatc ccgcacgacg
     1921 ccggcgagat ccggtggaac gggcgcatcg tcgaggatcc gtcccgcttc ttcgttcctc
     1981 cacgaacggc gtatacaccc cagaccccac gtctctttag cgagtcgctc cgccagaaca
     2041 tcatgctcgg gttgccggag cacgaggtcg acctgccggg cgcattacga tcggcggtca
     2101 tggagcagga cctgttggcg ctggaagagg gcctggacac ggtcgttggc gcacgcgggg
     2161 tcaagctgtc aggtggccag ctgcagcgca gcgcgacagc ccggatgttc gtccggccag
     2221 cccagctcta tgtcttcgac gatctctcca gcgcgctgga cgtcgagacc gagcgcatcc
     2281 tgtgggatcg cttctttgag cggccagagg caaccagcct ggttgtctcc caccgacacg
     2341 ccgcgctccg tcgcgccgat cagatcatcg tgctcgatgg gggccggatc attgcccggg
     2401 ggacgttgcg cgagcttctc gccacgtccc cagcgatgcg gcatctttgg cacggcgagc
     2461 caggcaagcc ggaggcgccc gccgaatccg gggcaggcat gtaaccctga ccgatggtat
     2521 ccgtggcgcg ggaaatgccg tgatggtcct gcctgaaagg agggccacac aatggcacat
     2581 tcccatcgtt ccgatactat caaccgacat gccccgagtc gacctgacac cattacctca
     2641 catgcacagc ccctgagcgg cgaagcagga gactacgatc gtctactcga ggagatcggc
     2701 gacgctcgcc tggtcctgat cggcgaggcg agtcatggca cggaggagtt ctaccgagcg
     2761 cgcgccacga tcacccgccg gctcattgag gaacgcgggt tcggtgcggt tgccgcggaa
     2821 gccgactggc cagacgcctt ccgtatcaat cgctacgtgc gcaatacaag cgacgatcgg
     2881 actccgctcg atgcgctcga cgatttcggc cgctttcccc actggatgtg gcgcaacacc
     2941 gtcgtccacg acttcgtcgg ctggttgcga gactacaacg acgcccatcc gaaccacaag
     3001 gcaggctttt acgggctgga tctctacagc ctgaacacat cggtccgtgc cgtcattgag
     3061 tatctcgaca aggtcgaccc cgccgcggct gaccgggctc gcgagcgcta cagctgtttc
     3121 gagcacttcg ggcaggattc ccaggcctat ggcatggcga ccagtctcgg gaccgcggag
     3181 ccctgcgaag acgatgtcgt cgctcaactg gtggagtttc atgtccgggc cgccgagctc
     3241 gccatgcgcg acggccaggt cgccgaggac gagttcttct ccgcccagca gaacgctcgt
     3301 ttggcgaaga acgccgaggc ctactaccgg tcgatgttcc gcgggcgtgt ctcttcctgg
     3361 aacttacggg atacccatat ggccgaaacc ctcgacgcat tgatcgacca tctcacccaa
     3421 cgcaacggcg caccggccaa gatcgtcgtc tgggcgcaca actcacatct cggcgatgcg
     3481 cgggcgacag agatgggcga tgcgggcgag ctcaatctgg gacagctggc gcgggagcgt
     3541 catcccaacg aggtcttcct tattggccag acaacccacg agggaaccgt gaccgctgca
     3601 agcgattggg accaaccagc cgaacgcaag cgtgtgcggc cgggactccc cggatcctac
     3661 gaggcgctct tccacgagtt cccgtacggc gcgtttcttc tctcgatgcg tgatgctggc
     3721 ggaatgttgc gaaccgaccg tctggagcgg gctatcggcg tcatctaccg cccggaaaca
     3781 gagcgactga gccattactt tggtgcccgc ctcgcggatc agttcgacgc ggtgatccac
     3841 tgggacagca cgacggcact caccccactg gagccgactg agcaatggga tcggggagag
     3901 gcgcccgaga cctacccctc cggtgatgaa ccgctgtcct gatcatcgcc aggccgacag
     3961 gggaagtgcc catgcgcgaa tcaccgcagg acatcaccat cccgatccgg agcgccagcc
     4021 tctacggtat gctggcgctc ccgccgggtg cgacaggcat cgttgtcttt gcccatggca
     4081 gtgggagcag ccgcttcagc cctcgcaacc atgaggtggc ggcggcactg cagcaggcgg
     4141 gtttcgctac cctgctcttc gacctcctga ctgagtcgga ggaggccgtc gaccggcgca
     4201 cccggcagca ccgcttcgat atcccgctcc ttggcgagcg cctgatcgcg gcaaccgact
     4261 ggctgagcga tcaggcagcg acgggtgaga tgaatattgg ctacttcggg gccagcaccg
     4321 gcgcggcagc ggccctcatc gcggctgccg accggcccaa cgtgcatgcg gtggtctccc
     4381 ggggaggccg accggatctc gctggagagg cacttggacg cgttcgatcc cctaccctgc
     4441 tgatcgtcgg tgagctcgat acagcggtta tcgagatgaa cacgagggcg aaagcgaagt
     4501 tgggcggccc atcggagatg gtcatcgtgc cgggcgcaac ccacctcttc gaggagcccg
     4561 gcacgatgga gcaggtgacc aagcacgctg aaggctggtt ccagcgctac ctcgccagcg
     4621 gggaacagat ggcatcggag tgagacgaag cacccaagat gatcacgatc atgatcattc
     4681 gcgtagcgtg cgctgatcgt gttgggagac gggacttgcg tggaccctct atccccattg
     4741 ctctatatac gcagcgggga ggatgaagtg tgccaccgta ggcggtctgc agttgctcga
     4801 ttgcctgtgg agcggtgagc cggtgacctt cgagggtgag cactatcgct atgtcactcg
     4861 tgtgccttgt cagattggag tccgtgagcg ccattgctgg gccaattgcc tgcctggtgc
     4921 gcctgaatcc tgcaccggtg cccacggaca cgagcccata cgactacttc cagctgtcaa
     4981 cggcacatgt cacctatcca gatgtagcac gctgtgagga gacacatgct cgatccgtcg
     5041 catcaacaac gtgcatatcg tcttcgtttt gaatggggca tggcaggcgc tgagaccgtg
     5101 gcgcgggact cggatatcgc gatcgtggtt gatgtcctgt ctttcactac cacactcagt
     5161 gtcgcgctgg atgctggcac ggtcgtcctt ccctacggat ggaacgacga gtctgccgcc
     5221 ctctatgcac gcgaccacga tgcggtgctc gcggcgggcc gaaccaacgc gaggccaggt
     5281 gatatcaccc tctcggcgcg atcgatccag gatgcatcat tgatatcccg gctcgttctg
     5341 ccttctccca acggctcggc aatctctcat catctcgcgt ccaccgcatc aaccgtgctt
     5401 gcgtcctcgc tcaggaatgc cagcgctgtc gcatgctgga tcaacgacaa ccacgacgct
     5461 gaatcgacca ctattgcggt gatcgcggct ggcgagcagt ggctgggcgg gacgctgcgc
     5521 ccggcaatcg aggatctctg gggagcggga gcggtgctct cgcatctcca ccgtagcggg
     5581 tggaccgacc tctctcccga agccgaggtg gcacgacaca gctacgagca ggcagcgggc
     5641 gacatcctgg ggcaacttcg tgcctgcgca agcggccggg agttgatcgg gaagggattt
     5701 ccagatgacg ttgtgatagc ggcggagttg gatcagagcc ggtccgtgcc gatccttcgg
     5761 gaacgccagt tcatcctcgc ctgaggattc cggcctccat cggcccgtgc gcgaattgca
     5821 cccgatagcc ttccggcgac ttggggaaga gggccggagc tacgagcagc gtttgggtat
     5881 ctgctatcgt ggtgcagata cagattcgac cgacactatc cggatacgcc atgtgaccgc
     5941 atccccaatc gttctctccg aagccgaccc cgcaatcgtc gtggatcgcc tgcgcaagac
     6001 ctatgtcgtc cccgagcgcg agggagggct taaggcagcc gtcggcagcc tcttccaccg
     6061 gaagacgaag gaggtcgagg cggttcgcga gatcagcttc tcgatcgcgc ccggcgagat
     6121 cgtcggcttc ctgggaccaa acggagcggg caagacgacg acgttaaaga tgctctccgg
     6181 cctcctccac cctacttcgg gcactgccgg cgtcctgggt catacgcctt gggaacggga
     6241 ccgggcgtac ctgggccaga tggcgctcgt catggggcag cgcaaccaac tgacctggga
     6301 tataccggtc gtggattcgt atcagctaaa caaggcgatc ttccggatca gcgacgacga
     6361 gttcaggagg cgcctcgatg agctgaccga cttgctggac ctcggtgacc tgatccggaa
     6421 accggtgcgc aacctctcac ttggcgagcg catgaagtgc gagatcgccg gatcgctgct
     6481 gcattatccc agggtgctct tcctcgacga gccaacgatc ggcctggatg tcgcgatgca
     6541 gcgccgcatc cgatcctttg ttgccgagta caacgggcgc actggtgcct ccgtcatgct
     6601 gaccagccac tacatggctg acgtcgaggc gctctgcgag cgcgtcatcg ttatccatca
     6661 cggaacgctt ctctacgatg gcggcctgcc gggactggtg agagccttct caccgcacaa
     6721 gacgatcatc gtggagctgg aggaaggcgc cgagatttcc cgagaccacg tcactaccct
     6781 cctcccgggt ggcggcgagg tggtcgagca aacgccggtc gggcttaccc tgcgcgtgcc
     6841 gaaggcggag accgcccgtg ttgccgccca ggtactgtcc gcgctgccgg tcttcgacct
     6901 cacgatcgag gacccgccaa tcgaggaggt catcgaagcg gtcttcgcca gccccacaag
     6961 cgaccaccat agcgagccca cgctggtatg agccgccccc agatccctga atcctcggca
     7021 tcaacgggac cagacttcga ccgcctgtcg ctgaaggtgc tatgggccta ttactcggga
     7081 aagttgaaga tcgacatcgc gaacaatctt gcctaccgtg gcgcggtcgc gatctgggtc
     7141 ctcagcaccg tggtgcaacc gctggtctcg ctcgttgtct ggcgaactgt cgccgggtcg
     7201 gagggtggca gtgccggcgg ttttaccgcg aacgagtaca cctcctattt tgtcatcgtg
     7261 atgctcgtca gccacatgac gttcatctgg cacatgtggg agttcgagtg gcgcatccgc
     7321 acagggttct tctcgcccat cctcctgcga ccgattcatc ccatccatca tgatgtctcg
     7381 gagaacctaa gctacaaggt cgtcgggctc atcggcgtga tcccggccgc gattatcctg
     7441 gccgtcgtct tcgatgccga cttcagcggg acatcagtca tcgacatact cgccttcttt
     7501 ccggcactga tccttgcgat ggcattgcgt ttcctcgtgg aatggacgct ggcccttgct
     7561 gccttttgga tgaccaaggt ctcggcgctc aacaacctct tcgacgtctt cttcctcttt
     7621 cttggcggca cattcgcgcc gctgagtgtg atgccgggct ggatccaggc ggtctcctat
     7681 gtctccccct tccgctgggc gatcgctttc ccggtcgagg ttgcccttgg gaaccgttcg
     7741 gggctggaaa tcctgacggg atacgtgatt caactcgtct ggatcacgct ctccgtcatt
     7801 gttttgcgcc tgatgtggac acgggccacg cgccgctact cagcagtagg agcgtagcga
     7861 catgcctgca tccgaccgcc cgcatgagga gcgacttgcg ggtgggttca tcaacgaggt
     7921 cgtccgcgtc ggggacacgg tgcgccggtc gggttccctg aacaccgagt tcgtcgcccc
     7981 gcttctgcag catctcgacg aatgtggctg gagcggtgcc ccgcgtttcc tcggcatcga
     8041 cgagcacggc cgcgagatcc tgagtttcat cgagggtgtc gtcccagtcg acgaggatat
     8101 cccgccatcg ttctcgacgg acgaaacgct cactgcgctc gcgcggatgg tccgcgagtt
     8161 ccatgacctc accgccggga cgccgctcgc cggcacggaa gaggtcgtct gccacaacga
     8221 tctctcgccg aagaacacgg tttatcgcga tgatgccgga atcatgatgc cggtcgcgct
     8281 catcgactgg gacctcgccg caccgcgagg ccgcatctat gacgtcgggc acatctgctg
     8341 gaaatacctc aatcttgggc catccgtcac cgatctggac gagtgtgccc gcaagattca
     8401 cctcatctgc gacgcctacg ggatcgaaga cctgagcggt gtgattgacg cgatcatctg
     8461 gtggcaagag gcatcgatct ggggcatcga aaccggcgcg aaggccgggg acaaaggcat
     8521 gatgcgcctg cagagtgcag gcgtcctgct ggagatccgg gccagcgtca catgggtgca
     8581 gaagcaccga acggcgttgg agcagtagct atcacgcagc agacaaatca tctttactga
     8641 aggatcgtaa cgaagggcaa gctgtgcacg cgctgaccgt tctcaaaacc tacctccagc
     8701 tcgggctgct caacgtcatg cagtatcgcg ccgatttctt cctgcaggtg ctgggcacga
     8761 tcatcggctt cgcgacgtca ctcatcggca tcctcgtcat cttcaaccgg acgaccgacc
     8821 tgaagggctg gggccgcgat gacctgatcg ttctcgttgg aatccagatc cttgtcgcgg
     8881 gtttgatcaa cctggtgatc cggcccagca tgcagcagct catggagggc atccggctcg
     8941 ggacgcttga tttcatgctc accaaacctg cggactccca gctattggca agcatccaga
     9001 tggtcaatcc cgcggggatc caggatgccg tcttcggggc cggcgtaatt ctcgttggac
     9061 tttcgttatt ggatgcggcg atctccccgc tcgcggtcgt cctcttcctg ctcgtcctca
     9121 tcgccgggtt gatcatcatc tacagcttca tgctgatcct ctccacatgc gccttctggt
     9181 tcgtgaagct ggacaacgtg ctggtgatct tcaacaccat gttcggcaac gccggtcaat
     9241 ggccgatcac catctatccc ggctggctac gggcctcgct gaccttcctc gtgcccgtcg
     9301 cgtttgcggt gacgattccg gcgcagagcc tgaccggtcg actggacggg tggaccgtgg
     9361 tcggcacgtt cgctctcgcc ttcgcgttct tcgtggcctc tcggctcttc tggcgattcg
     9421 cgctccgcca ttacaccggc gcctcggcct aaccctcttc acatctcccg aaatctatcc
     9481 gatgatgcga cggatggtgt gcgtggcggg agcatggaga cagagagatg caacgcggga
     9541 gaccggagca gttcgtttca tcggtgacga aacccgacga cttcaatgcg ttctgggacc
     9601 aggttcaggc ggagctcgat gacatctccc tcgaaccggc aatgaggcac gtccctgagc
     9661 gatcgacggc tgaggtcgat gtctatgaga ttcactacac cagcctgcat tcgctgcgca
     9721 tcgccggttg gtactgtact ccgaaaccgg attatccggt ttcaccctac ccgggactgg
     9781 caatcctgcc cggatatgtt tccgagccga cgctgccaaa gtcctgggcg aagctcggct
     9841 acgcggccgt tggcgtggcg ccacgcggca agttacgctc caacggtcag tacaaccccg
     9901 gctatcccgg tctgctggtc gatcgcatcg ttgacgagca gacctatggc tatcgcgggt
     9961 tctatgcgga cgcgctacgg gcgatcgact tcatgttcac caggccggaa atcgatccct
    10021 cccggatcgg cgttcacggt ggcagccagg gcggtgccct ttcgctggtg accgcggcgc
    10081 tacggccgga tgtcgtccgc tgcggtgcgc ttgccgcccc ctacctgact ggatttatgg
    10141 atgccgccgc actcactcac tcctacccct atgaggagat gaatgagtac ttccgcacct
    10201 acccggagcg aatcgatcag gtacggaagg tggtcagcta cttcgactgc ctgaatttcg
    10261 cgccgaagat caccgcgcca ctgctgatct atgtcggttt ggcggatgac atctgcccgc
    10321 cggaaacagc gttcaacctg agggaacggc tcacctgtga tgtcaccttc cgtgcccacg
    10381 ccggttgcgg ccatgaggcc ggggtgtatt gggaattgcc gaaggtggag gcatttctcg
    10441 ccgatcacct ccggccagcg gcccccgccc gcctgatgac cgatggcctg gaggaagtat
    10501 catgagtgca gacctcgcac aggagatcga tcaatggtgg gagcgtcttg acgacgacct
    10561 ctgccccttg gaagcgaccc cggaattcga gcatgatgcg ctgcgttcac acgacctctt
    10621 cgacacatac cgggtacgca tgaccagtct cgatcgctac atcatcgcgg cctggctcag
    10681 tgtgccgaaa ggtgatggcc ccttccccgc cgccatgtac acaccaacct acatgagcgt
    10741 cgtcaacccg gcgccgtacg agttccggat ccgcgccata acgatgaacg tgatcagccg
    10801 cgggcaacgc ggcgcggatc ggccgtatgc ggctgcattc cccgggcacc tcaccgttgg
    10861 catcgatgat ccggaccgct atatcttccg gggcgtggtg gcggatacga tccgcgcgtg
    10921 ggaggtgctc cgcaaccatc cccaggtgga taccgaccgc atggcgatga tcggaaccga
    10981 cctcgccctg cttgtgaacg caaggagagc cgatgcaaag gcggtgcatg tgaccggcag
    11041 cttctggtac cggatgaagg agattgcggc ggggacggaa gcctatccat ttgaggagat
    11101 caacgatcac ctccggatgt ggcccaatca ggaagaggcg gtcgcccgca cgctggccca
    11161 tttcgatccg caataccagg cggagaggat aaccgccaga gtgctcttga atcgcgatca
    11221 ggcaagtccc accgggaatg atgcctggtt cgctcccctc gcctcccgcc tggccacagg
    11281 gccaacgttc ctcgatatga cgcaccgtgg tcaaaccgat tacgacgcga tggatgcctg
    11341 gctctcgaac gagctcggga tgccagcata cccgcgaagc tggacgccgc aggatctggg
    11401 agcgtggtcg gtatgaaacc cgaagggatg ccacaggact ggctgcggga gcttggcctg
    11461 cgacccgtga tcaatgccgc cgcaactttg acatccctgg gtggctcgat catgccaccc
    11521 gaggtggtag aggcaatggc cgtcggtgcc cggcactttg ttgattaccg ggacttacag
    11581 gcatcagttg gtgcgcgcat cgcagccctc acgaacaacg aggcggccta cgtaagctcc
    11641 ggtgctgcgg cgggaatcac cctttccatc gccgcctgca tggcgggaaa ggacccggat
    11701 ctcatacgca gcttccctgg ccctgatgcg tttcaacgca acgaggtcct ggtctaccgg
    11761 tcgcaacgaa acggctacga ctacgccgcg agcatgaccg gcgcgaagat gatcgaagtc
    11821 gccagcgagg gtgacccgct tgccgcgcgg atcaatgagc ataccgccgc cgtactttgg
    11881 ttcgcgggca cgcgcctgcg cagcgatacg ccaccgatcg aggagatcat cgcaatcgcg
    11941 catgaggtga acgttccggt gatcgtggat gctgcagcgc agattccacc agcgtcgaat
    12001 ctctggcact tcacccggga actcggcgcc gacgtcgtca tcttcagcgg tggcaagggg
    12061 ctacgaggtc cgcaagcaac aggtctggtc atgggcaggg cggcgttgat tgaggccgtt
    12121 gccgccaatg cctccccaca ctactccatc ggacgcccga tgaaggtcgg caaggaggag
    12181 ctgttcggca tccttgccgc cgtcgaatgg acactcgcgc aagatgaggg ggccgtcatc
    12241 tatgcctacg aagaaaccgt gaagtgctgg atcgatgggc tggaaggact acccggggtt
    12301 gagatcagcc ggggctttcc cagcgaagcg ggtcagcccc acgcgcgagc aatcgtgcgc
    12361 gtcctcccta gtgcccggcg ggaccgggat gcagttcatg acgccctgtg ggacatgaac
    12421 ccgcgcatag cagtgctaaa ggagggcgac gacgtgctcg ccctcaaccc acagaccctc
    12481 gaacccggcg aggcggagat cgtgcttgat gcgctgcgtt cagtactcga gttgccgccg
    12541 ggctgacgct ttgcccgaag ggacagctcc gaatcgtgcc aacgtacctc cttcgctcat
    12601 gtgggagatg actcaagcac gggctcaagg agatccggtt catcggcggt ttctggatgc
    12661 ttccgtagca cgatcaccgc aagtgaggtc agcactcctg tcagcccgca gatgagatag
    12721 aggaccgacc agccggaccg ctgcgccagc caaccgccca acgggaagaa cacgatcatc
    12781 aggcaggaga agagccagga ttcgatcgag agcaccgtcg cgcgctgggc agagggcaac
    12841 tgggcgttgt accaggcgga catccgcgtc tccaccacgc cactcgcgaa ctgggcaaga
    12901 acgaaggcag tgatagccag gacgacgctc tccagcccga tcaggacgac gccggccccg
    12961 gtcgtcacgg ccagcaccgg ccattgccaa gcgaagctcc cgcgtagctg tctacccacg
    13021 gtggttcctg cggcgatcgc gaggaacatc gaacccacca gcaacccgat ctgcccattg
    13081 ctgagaccac gatcggagaa cacggcctgg acatagagtt cggcaacggt caggctcccc
    13141 cagaagacgg cacctaccgc gagcatcagt gccacggcat gccgtgtccg cagcagggcg
    13201 tggacgtcat gaacatgggc ccggatcccc ttcttccgtc ccgagtggct tcgctcgcgt
    13261 gccggttcat ccagtccgaa ggcgagcagg atgccgcaga ccgcgaagaa tcctgagcag
    13321 gccaacggga tcg
//
LOCUS       DASYWM010000010         5401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_206866, whole genome shotgun sequence.
ACCESSION   DASYWM010000010 DASYWM010000000
VERSION     DASYWM010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206866"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGR29_00395"
     CDS             <1..220
                     /locus_tag="VGR29_00395"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEV2072075.1"
                     /translation="RKHVLGGMVFGTGWAITGACPGTASTMIGGGSVLGIVLAVGVVA
                     GITLRDMIVSTPQAAPLAVTDPVTAQPS"
     gene            complement(658..734)
                     /locus_tag="VGR29_00400"
     tRNA            complement(658..734)
                     /locus_tag="VGR29_00400"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(698..700),aa:Glu,seq:ctc)
     gene            complement(802..1311)
                     /locus_tag="VGR29_00405"
     CDS             complement(802..1311)
                     /locus_tag="VGR29_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2072076.1"
                     /translation="MSDYLLPLSRTRLTLESVNTGIPFMNLHARLSGGTTSAETAQVR
                     GRNTSRPTILVVDDEPQIVGFLSDLLEDEGYRVCNACNGREAMHILQRESPSLVISDL
                     TMPDVDGHELVYYIHREISGQAPKVILMSAIERCSPNQGVPFLHKPFDINDLLDLVDS
                     TLGEGALVS"
     gene            complement(1355..2002)
                     /gene="pdxH"
                     /locus_tag="VGR29_00410"
     CDS             complement(1355..2002)
                     /gene="pdxH"
                     /locus_tag="VGR29_00410"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEV2072077.1"
                     /translation="MNEPLSSMRMSYELARLGDKDADRDPMAQFSRWFAEVLETHAGE
                     ANALILSTVSPNCVPSSRTVLMKQFDHRGFVFFTNYESQKGREIAANPHVAALFYWSS
                     LQRQVRIGGIATRIGDEESDAYFQTRPHGSQIGAIVSPQSQEIPNRAWLEDRFQQAEE
                     ALERGEILKRPNHWGGFRIEPNSMEFWQGRPNRLHDRVVYRRGVDGSWSMHRLAP"
     gene            2301..4730
                     /locus_tag="VGR29_00415"
     CDS             2301..4730
                     /locus_tag="VGR29_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEV2072078.1"
                     /translation="MVTLRQAKPRREDEGIGLDPRDIGTLQAIERRLLWLATQIVHYA
                     NNVRENPDKPTKVGGHQASSASVVTILTALYFHFLRAGDRVSIKPHASPVYHAAQYLL
                     GNLEQKYLTMLREFGGLQSYPSRTKDPDPVDFSTGSVGLGAVAPVYAALASEYAHLHF
                     GDVTSGRFIALMGDAELDEGNVWETVAEEAVQGLGNVIWIVDLNRQSLDRVIPGIKAS
                     RLKRLFAGAGWHVLEAKYGTSLEAVMESPRGGALRDRIDDMPNEEYQALIRVTDGAEL
                     RRRLADVADANRHKAILASVEHVSDADLPTLISNLGGHDLPKLIQLLNEADSIEHCPV
                     VIFAYTVKGYGLPMAGDPLNHSQLLTEAQMEAVRQDMGLEPSQVWDAFDEHSEPGRLI
                     ARRVEELSSERQPVRLALPNVPVGIEVRHPAKTSTQEAFGRTLIRLGEIPEIGERIVT
                     MSPDVATSTHLAGWINKAGVFSLEEQRDYETATQRMLQWKPSPMGQHLELGISEMNLF
                     MALGQFGLAEELTGQPLIPIGTVYDPFICRGLDALIYSLYIHSRMIFAGTPAGVSLSP
                     EGGAHQSSVTPSLSIELPNLYFYEPAFGKEVDWILLEALRQCCDREHGLSTYLRLSTK
                     PIDQSLMSEPLERLGEDELHRQVIAGGYRLIEGGISAPDADARDTVQIAAGGIMIPEA
                     VAAAKRLHAEGVAANVINVTSAQKLYAGIRSSRQAQLDDAYVALNHGHIETLLPRHER
                     RAPIVTVQDGASHSLAFLGGIWGTPVVPLGVDQFGQSGARKDLYRVMGIDADQIFNAA
                     LLALDLARELD"
     gene            complement(4747..5331)
                     /locus_tag="VGR29_00420"
     CDS             complement(4747..5331)
                     /locus_tag="VGR29_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072079.1"
                     /translation="MAILLSLLLPLSILFSWLRLIGVGSHRLEGAPIGVMPIPLLARL
                     YMHAMPIAISLATAILLAALGVISWLWLPVPVISGVLLVSVPLRYTLSAAGIRRSFGG
                     FRRWTEFAGVERAPGGARLKPLPNTHRAHIWLSGSRGDDDFLQLMRVLIRNAYKGKSD
                     ITIFPGSTVVSSEPTDSVPPALPQIAAFQRSEPV"
BASE COUNT         1117 a         1549 c         1654 g         1081 t
ORIGIN      
        1 acggaagcac gtgctgggcg gtatggtgtt cggtacgggg tgggcaatca cgggggcctg
       61 ccctggcacg gcgtcgacca tgatcggcgg aggcagcgtc ctgggcattg tgcttgccgt
      121 gggtgtagtt gccgggataa cactgcgaga catgatcgtc agcacgccac aagccgctcc
      181 cctggctgtc actgatccgg tcactgcgca accaagctga ccggccaaat ggcgcaacag
      241 cgttactagc cagacccgga gacggcaacg ctctcaatta cccagcgacg ctgctacatg
      301 agcactggtt cgcctcccct ccggttcctg atttgatatt ccccaatgta ggtgcttgac
      361 aggcagtcat gtgtacgtat atagtgttcg tacagtcccg aggggctggg cacatggcat
      421 cggccaaccg gcgttcatat ggcggacatc gctgcggggc tttgttcaac caccaggtga
      481 acgacggggt gcaggaggcg aacgatacgc gttgcggggc gtcgtatcga aacgatcttg
      541 gacacggcat ggtgcttgtg ccccgcctat cgggactttc gttttacacc tctaatcact
      601 ccaggacgag aacgaaaaag gaccctcctg ccgcggcagg aaggtccttt gcattgctgg
      661 tagcccgtag gggattcgaa cccctggtct ccgccttgag agggcggtgt cctaggccgc
      721 tagacgaacg ggccgtaaag caacggtcgg tatcttacta cacggcccct gcgcggtcaa
      781 accgtctggt ttcaattcat gtcagctgac gagtgctcct tcacccagcg tgctgtcgac
      841 gagatcaagc agatcgttga tgtcgaaagg cttgtggagg aacggtacac cctgatttgg
      901 ggaacatctc tcaatagcgc tcatcaggat aacttttggt gcttgccccg atatttcccg
      961 atggatgtag tacaccagtt cgtgcccatc gacatcgggc atggttaggt cagagattac
     1021 caggctaggc gattctcgct ggaggatatg catcgcctct ctcccgttac aggcgttgca
     1081 tacgcggtag ccttcgtcct ccaggaggtc cgagagaaaa ccgacgatct ggggctcatc
     1141 atccactacg agaatggtgg gtctggacgt gttccgaccc cgaacctggg cagtttctgc
     1201 tgatgtggtg ccgccggaca ggcgggcatg gagattcatg aaagggattc cagtgttgac
     1261 cgattccagc gtgagcctgg tgcgtgataa cggaagcaga tagtcactca cgcgacttct
     1321 tgccgggaac gcaactcctg tgccagcagg agggctaagg cgccaggcgg tgcatgctcc
     1381 aggaaccgtc aaccccacgg cgatagacga cccggtcatg gaggcggttc gggcggccct
     1441 gccagaactc catgctgttc ggttcaatgc gaaatccgcc ccaatggttt gggcgtttaa
     1501 gtatctcacc gcgttcgagc gcttcctcgg cctgctggaa ccggtcctcg agccacgcgc
     1561 ggttgggaat ttcctggctt tgcggtgaca caatggcacc gatctggctt ccatggggcc
     1621 gggtctggaa ataggcgtct gattcctcgt cgccgatgcg tgtcgcgatt cccccgatgc
     1681 gtacctggcg ctgaagtgat gaccagtaga agagtgccgc gacatgcgga ttcgcagcga
     1741 tctccctccc cttttgactc tcataattgg tgaagaacac gaatcctctg tggtcgaact
     1801 gtttcatcag caccgtgcgc gaggatggaa cacaattggg gctgacggtc gaaagaatga
     1861 gcgcattggc ctcaccagcg tgtgtttcca gcacctcggc aaaccacctt gagaactgcg
     1921 ccatcgggtc gcgatcggca tccttgtcac ccaggcgagc aagttcgtag gacattcgca
     1981 tcgaggaaag gggctcgttc atgtatgctc ttactcaatg tggtcggatg caaagaaacc
     2041 gtttggagag gggcacaacg gggcgctggg atcgctcccc gatcggtagc agagcaagca
     2101 tacgcgagtt cctgcaaata cgatgagccg tcccctcgta tgtactgtag gtacaacctc
     2161 ccggcagcgc cacagtgatg gtgagaacag aacctactcc cgtgacaggc ttcgaagttt
     2221 gaggacgcgt ggccgcgtga ttcgatgctc ataggcccca gcgggacagg cggcaggacc
     2281 gggacaggaa ggacgagtca atggtgacgc tccgtcaggc gaaacctcgc cgcgaagatg
     2341 aggggatcgg tcttgatccc cgcgatattg gaacgctcca ggcgatcgag aggcgactcc
     2401 tctggctcgc tacgcagatc gtgcattacg ccaacaacgt ccgcgaaaat cctgataaac
     2461 caaccaaggt aggtggccat caggcgagtt ccgcctctgt cgtcactatc cttactgccc
     2521 tctacttcca tttcctgcgg gcgggtgatc gcgtctcgat caagccccat gcgtcgcctg
     2581 tctatcacgc cgcccagtat cttctcggga atctggaaca aaagtatctg acaatgcttc
     2641 gcgagtttgg agggctgcag tcctaccctt cccgtacgaa ggatccggac ccggtcgatt
     2701 tctcaaccgg ctcagtgggt ctaggtgctg tcgctccagt ctatgcggcg ctggccagcg
     2761 agtatgccca tctccacttt ggtgacgtga cctccggacg gtttattgcc ctcatgggag
     2821 atgcggagct cgacgaaggc aatgtctggg agaccgtggc cgaggaggcc gtgcaggggc
     2881 ttgggaacgt tatctggatc gtggacctca atcgccaaag cctggaccgt gtgatcccgg
     2941 gaatcaaggc atcgcgtctg aagcgactct ttgccggtgc tggctggcat gtgctggagg
     3001 ccaaatacgg aacatctctc gaagcggtga tggagagtcc gcgaggcggt gcgttgcgag
     3061 accgcatcga cgacatgccc aacgaggagt accaggcgct tatccgtgtc accgatggtg
     3121 cggagctacg ccgccggctg gcggatgtcg ccgatgcaaa ccgtcacaag gcgattctgg
     3181 cgtccgtcga gcacgtctct gatgctgatc tcccgacgtt gatttccaat ctgggcggac
     3241 acgacttgcc gaagctcatc cagctcctca atgaggcgga cagtatcgag cattgccccg
     3301 tggtgatctt cgcctatacg gtgaagggat acggactgcc aatggcgggc gatcctctga
     3361 accactccca actattgacc gaggcgcaga tggaggcggt gcgccaggac atggggctgg
     3421 agccctccca ggtgtgggat gcgttcgacg agcactcgga acccggaagg ctcattgccc
     3481 gtcgggttga ggaactctcc tcggaacgac aacctgtccg gttggcgctg cccaatgttc
     3541 cagtggggat cgaagtccga catcccgcca aaacgtcgac ccaggaagcg ttcggtcgta
     3601 cattaattcg ccttggagag attccggaaa ttggcgagcg cattgtcacc atgtcgccag
     3661 acgttgccac ctccactcac ctggcggggt ggatcaacaa ggcaggcgtc ttttctctcg
     3721 aggagcagag ggactacgag acggcaaccc agcgaatgct gcaatggaag ccgtcgccga
     3781 tggggcagca tcttgagctc ggcatctccg agatgaacct cttcatggcg ctcggacaat
     3841 ttggccttgc agaggaactg accggccagc cgctgatccc gataggaacc gtctacgatc
     3901 cctttatctg tcgtggcctg gatgccctga tctacagcct ctacatccac tcccggatga
     3961 tcttcgctgg cacgccggcc ggggtgtcgc tcagcccgga gggaggcgct caccaaagct
     4021 cggtaacacc ttctctgagt atcgagctac ccaacctcta cttctacgag ccggcgtttg
     4081 gcaaggaagt tgactggatt ctattggagg cgctccgcca gtgctgcgac cgggagcatg
     4141 gcctttcgac ctatcttcga ttgtccacca agcccatcga ccaatcgctc atgtctgagc
     4201 ctctcgagcg ccttggcgag gatgagctac accgccaggt cattgctggc ggctatcgcc
     4261 tcattgaggg tggtatctcg gctcccgatg ccgatgcgcg cgatacggta cagatcgccg
     4321 ctggcggtat catgatcccg gaagctgtag ccgctgcgaa gcgactgcat gccgaggggg
     4381 tagcggcgaa tgtaattaat gtgacaagtg cccaaaagct ctacgccgga attcgttcca
     4441 gccgccaagc ccagctggat gacgcatatg tggcactcaa tcacgggcat atcgagacgc
     4501 tcctgccccg ccacgagcgg cgagccccga tcgtcacggt ccaggacgga gcgtcgcatt
     4561 cgctggcgtt cctgggaggt atctggggta caccagtcgt gcccttgggt gtagaccagt
     4621 tcggtcaatc cggtgcccgc aaggatctgt atagagtgat ggggatcgac gcggaccaga
     4681 tcttcaacgc ggcgctgctc gcactcgatc tcgcccggga gctggactag agccggtcca
     4741 gagtcctcag accggctcac tccgctggaa cgcagcaatt tggggaagag ccggagggac
     4801 ggaatccgtg ggctcggatg acaccaccgt gctccctggg aagatcgtga tgtcggactt
     4861 tcccttgtag gcattgcgga tcaggactcg catcagttgc aggaagtcgt cgtccccacg
     4921 cgatcccgac agccagatat gggcgcgatg tgtgttcgga agtggcttaa gacgcgctcc
     4981 gcccggagcc cgctcaaccc ctgcgaactc agtccatcgg cggaagccac caaagctgcg
     5041 gcggataccg gcggcagata gcgtgtaccg aagtggcacg ctgacaagaa gcactcccga
     5101 gataactggc acgggtagcc agagccacga gatgacacca agtgccgcaa gcaaaatggc
     5161 cgtggcgagc gagatggcaa tcggcatcgc atgcatgtag agccgggcaa gcagcggaat
     5221 tggcatcacg ccgattggag caccctcgag ccgatgcgag ccgacgccga taaggcgcag
     5281 ccaggaaaag agtattgaga gtggaaggag aagagacaga agaattgcca tacgtgttct
     5341 ctgggagtta agtgcgtgca ggataaggcg agagtgaggg cgaacgtagt ccggacgtca
     5401 a
//
LOCUS       DASYWM010000011        17382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_582192, whole genome shotgun sequence.
ACCESSION   DASYWM010000011 DASYWM010000000
VERSION     DASYWM010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 17382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17382
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582192"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VGR29_00425"
     CDS             <1..237
                     /locus_tag="VGR29_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072080.1"
                     /translation="PDGFPIKGNASSRIFHPKESPSYENTVAEIYFASNEVAEQHGYR
                     LPKTMQQVGATAANSAAGLAQNAMDAAGSDSEEG"
     gene            238..714
                     /locus_tag="VGR29_00430"
     CDS             238..714
                     /locus_tag="VGR29_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV2072081.1"
                     /translation="MSLAKVTIIGNLGRDPETRYTPSGALNVQFSVAASRRWRDSQGQ
                     EQENTTWFRVTAWGRLAETLVNLNERGALVKGRQVYVEGRIESREYTGTDGQVRTSLD
                     VNANEVQLLGARQDGQGGEGSSGGQQYGGGYGGGQQRQRNDFGDQGGSDDLNEVPF"
     gene            1137..1319
                     /gene="rpsR"
                     /locus_tag="VGR29_00435"
     CDS             1137..1319
                     /gene="rpsR"
                     /locus_tag="VGR29_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV2072082.1"
                     /translation="MEHVDYKDIGRLRRHVGERGKIEPRRKLGTCAKHQRSVTVAIKR
                     ARYIGLLPFTNSAPRG"
     gene            1472..2923
                     /locus_tag="VGR29_00440"
     CDS             1472..2923
                     /locus_tag="VGR29_00440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV2072083.1"
                     /translation="MSDRFKRPSGTRTPLGDRGKTRLTSPPRRASRRQKETMYQRIFY
                     IGLIAAGVLILAILGIGAFWEYQVKPNQVVASVNGEEITREEYWKYQNIALYNQARTY
                     EDYALQVTGQQQTQFLLYAAQFDQMREEVWGSSDVSEATVRQMIEDRLYVAGAEEMGI
                     DLSEPVLEDYALNAFSPPGQSVVTPIPTPTMIPQRAAWATETQQALQTQQAAALGTPP
                     AAAGATPVGTPGATPAASPVATPDIEAARITASSEFELFQEEVFDDADISLDEYMELW
                     VKPQAARQLVDAKIISDVPQRAEQVQAQHILVATEDLANQTYEQITSGASFDEVAKTT
                     STDTITAPTGGKLGWITKDQMPDAFTEVAFSTEPGEVAGPVKTEYGWHIIKVNDNAED
                     RPLTEAQYSLAKTEAVSNWIEQQREKSEISSDYYEPTPEPTSEQFAPPPDAPTPIVAT
                     PIAAPDLSATPVAGPSLKPDASPVGTPMATPGQ"
     gene            3063..4109
                     /locus_tag="VGR29_00445"
     CDS             3063..4109
                     /locus_tag="VGR29_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072084.1"
                     /translation="MSSRDGSDQSGRNHRSAPSDRGANPRKQSQRANQPSWDIDPAEI
                     DRYLSGRPSREQETKATARSRVRQDAPQGTAGQLNRLQRAVNDRRASHDARSEPTPGA
                     ASPGRQQFAEPDYDDEVNAGTSLTEPDYGSDESYEETSVPASPGRERSPRQAQRRTQA
                     SAAQPPRQRQQTPRQRQRPQEEYDDPSDVELYADDPYLTYDDERDWEVPTRRRTARSR
                     PKVTFTTPSLPKVSMPRSISGAELVNDIPSLALMGAAILSAAIMAIVVSNRLDLLPLT
                     IPTHVSASGLPENIRGRNALWSVPLLSIALSLMNIVTAWFLAKADMFAARFILAAALL
                     VQFIAWIALIRYLW"
     gene            complement(4185..5063)
                     /locus_tag="VGR29_00450"
     CDS             complement(4185..5063)
                     /locus_tag="VGR29_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEV2072085.1"
                     /translation="MCRDCGENAVFPTSFTYHRAQSIEDAVAVLEQNPDAKILAGGHS
                     LIPAMKLRLAAPETLVDIGRIAELRTIKVDADIHIGAMVTYNEIRDHMELATLFPILP
                     EAINVVGDQQVRARGTLGGAIAHADPAADLTAVFHALNGRISATGPSGEREIAADDVF
                     VDLWTTSLEPNELLTTIVIPRPAPGTKMAYAKHSHPASGYAVVGVAVVLEMSHGSVSD
                     ARVVITGATSTPTRATGVEAALNGKNLTSTTIADAASRASVDLEINGDTYASEEYRAH
                     LVNVMTRNALEQAAAR"
     gene            complement(5084..5605)
                     /locus_tag="VGR29_00455"
     CDS             complement(5084..5605)
                     /locus_tag="VGR29_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV2072086.1"
                     /translation="MEKPMSSTEVNTRHRVTMTVNGQERVADVEPRTLLVHFLRDCLG
                     LTGTHVGCDTSQCGACVVNVDGITTKSCTMFAVQCDGSNVQTIEGLSGTGSYHPVQEG
                     FWEKHGLQCGYCTPGMIMSSVALLERNPNPTEDEIRHGLEGNLCRCTGYQNIVRAVQY
                     AAEKMQKEPFSAD"
     gene            complement(5786..6973)
                     /locus_tag="VGR29_00460"
     CDS             complement(5786..6973)
                     /locus_tag="VGR29_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072087.1"
                     /translation="MKSPVFPGRSLLSATFDRDIMRLSDRGGASGLIGDRWSDVCAER
                     MAAWPGTTVRVLEGREIRVIRIVRLDDVPAIAHTASRHKLQNPDFIVVGSDARGQIIF
                     SVDAKFSIETAGASQVSGESLQSLLEIGPIITESVGEVSDDVTVEDGIFLAPDYSLTH
                     YVMGRRRGYRSLSVNMAQIALLPLTATGLLKPVEGSSLIPLFAGIDQLDMESRQSVLL
                     AQYYFRLVRAGIGCWYDQTGSLLVPKVKPTLDLDQVRQEAEGLAKDAVSGWEVIQRWD
                     SVAESVRSQRDLVNKMTAVPIVNRELREQLEKAALAASVTPPSLNKVRRRIGSWFRDQ
                     LIEDFGPIMPPVSNFSDVLQQLGRRARELHPDLERMTAEIISAMLQEMREDNSETTRS
                     GPS"
     gene            complement(7085..9142)
                     /locus_tag="VGR29_00465"
     CDS             complement(7085..9142)
                     /locus_tag="VGR29_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072088.1"
                     /translation="MPEPGDQPVDDSGRVEGRVWLVLLLAMCLWAAWRIGAFDLTNIV
                     TIQGREIEVPNVYAWVDHPFHATRAHLLLESLKEGDLLRWVGNHQGGYPVEFYPLGIP
                     WLDVALWALFLGTMPIIALHKLAVVAVFLLPAVSYWVLARGDRIHPGVALLATALHFA
                     VPGHWLNGGYTELVGWGLVTNVAGGSLALLASAALARFVVRREHAMGVLAVLAAAAGA
                     CTNPRSLFAVVIAAIAILATVTAAGDRQQAKARFTDAVSRIGLVGVLALLLAMPVVLA
                     LFRYNAEYFFLHYEFYEPVTELWTASMSAVSLPVLIMAIIGTGVAILGRPCTVAQSLA
                     VTLVAYVLFTMAVAASSWVPPLVEQLEAPRLMPYQRQLTMYFGAWALGAGLQKISQQI
                     PVWKRAYASSAVIMVVAVAALVIFVRPASFVPENFHGLTPVDTTGDDDFPLFMEAIEE
                     AESEREEGTSIFVVGNREDRAYWWHQQLWAPVASPGPFYYDDWLWYWHRDHRGPYDYR
                     NGYSFPNPGDALTAEYLRNNGISVVVVTDMSVPSGPSPRMAARTSPALALDTTIGQWE
                     IYSVVDPTSIVSNGDSMPEDTEIDNGDIVARFDDGGGTVVVRQNWFPRWEAFASGEKV
                     DITRRDDGYMELNVPPGPVSIELRYGVTLLDWVGRIAAIAGVAGTVGFTIRGRHLFAR
                     RRG"
     gene            complement(9135..9533)
                     /locus_tag="VGR29_00470"
     CDS             complement(9135..9533)
                     /locus_tag="VGR29_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072089.1"
                     /translation="MAQTPSPGSADDSRLADQSDEITQNSRARFGVWLVLSASFLGFI
                     AFGSVDARIWRTGQLYFRERGVVHVWGYFGRHLPEMPAQSLITALYYLSVSVMVGGTV
                     LGLWYFLHEDGDPSDPQRDIPAKTVPPDHA"
     gene            complement(9610..11115)
                     /locus_tag="VGR29_00475"
     CDS             complement(9610..11115)
                     /locus_tag="VGR29_00475"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2072090.1"
                     /translation="MENGHNPNGAKESTVGTTVVMGAGPAGLCSAYVLSKAGAPVTVV
                     ESAPFVGGLARTIRRQTEYGEFRFDIGGHRWFTKNDELNALFQEVVDDELLWVNRISR
                     IYFDGKFIDYPLKIGSALTSVGPVTAARAVVDYGRARLEQKFRPKELLSMEDAYVDQY
                     GQTLYELFFQRYSEKVWGMPCDQLSGDWVTQRTKGMSLMTAIKDAVIPSKGAVVSLID
                     EFMYPRDGFGRFSERMADSITDSGNEILLKHPVRRIVLDGNRVKGIEIQTENGIEIME
                     AENFISSIPMTVLCKITDPPAPDDVIEAANSLTFRDIITVNLMIKKRQVTNDTWLYVH
                     DRNILFGRFHEPKNWSPAMVPNDDFTSLVVEYFCTKGDHIWNMSDEQLVNQTVKHLVE
                     DLHFIKPEEVIGGFTLRATKAYPVYDIGYEEPLRKMKDYIRSIENLQFIGRGGSFRYN
                     NTDHSIETGLLAAKNILGEHHDLENVNADDEYHEIKRSPAQVADAELQAKS"
     gene            complement(11360..11785)
                     /locus_tag="VGR29_00480"
     CDS             complement(11360..11785)
                     /locus_tag="VGR29_00480"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV2072091.1"
                     /translation="MISVESWPQPAIRIWTLMQRFQKFLVVGAVGFATNQGALFLLHS
                     SFGLAVPVASPIAIFLSMIVTFYMNEVWTWHDRGTGRIISRAQTYVPINIGGLAINWG
                     ILTYLHENTSMHYLLANLIGAAVAAVWNFTLNNAITWRS"
     gene            complement(12050..13279)
                     /locus_tag="VGR29_00485"
     CDS             complement(12050..13279)
                     /locus_tag="VGR29_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV2072092.1"
                     /translation="MTSVLLVLAVCLGMGLDRAMIATGLVDASTQLTTSEEFTILEET
                     YDAIRNNYVLEEEISDEELMYGAARGMVDALGDEGHSVFLDPQEAEEFEQSSRGELVG
                     IGIQIDTEASPPVVIAPIPDSPALEAGLLPGDVILEVDGVSTEGLEPEEIGNRIRGEA
                     GTDVTLTLQHEGEVDTYEVTITRTKIDVNPVSWALLPNNVLWVQVSQFSSGATEGLQQ
                     AIREGQALGATSVILDLRNNPGGLVFEAMGIASQFLPDGSPLYQEVDAEGNIEVVRTV
                     GSNGVYQEGRLVVLVNEYSASASEIVSSSLMEAGRAPVLGEKTFGTGTVLLPFELSDG
                     SMAVLGTELWLTGEGDEIYMEGVEPTETVELEEGTRTALPVIYTSETGDEEIAPETFE
                     AIEDAQLHAAWEELEIP"
     gene            complement(13618..14553)
                     /locus_tag="VGR29_00490"
     CDS             complement(13618..14553)
                     /locus_tag="VGR29_00490"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEV2072093.1"
                     /translation="MSQHPKYLWWNGEPVAWHDATVHVSDIAWSATGAVFEGIRAYWN
                     GEQEELYVFRLHEHMRRLKDSSKMVRLPIDVTVDDLIENTVDLLRRNGSREDTYIFPL
                     VYFGGARTGRSDPAKLRGDLLITTDPRPSHLGTGVKQRAKTSSWTRISDNVMPPRVKN
                     ISNYRNGQLATYEVKLDGYDVALLLGPDGKVAEAPGACVMFMKNDQLITPDPASGILE
                     SITRDSIMTLAREDLGLTVIERRVDRTELYTADEIFLCGTMAEITPIVEIDRFLIGDG
                     GIGPVTHQLERLFTEAVRGNEAKRESWRTAVGASA"
     gene            complement(14640..15605)
                     /locus_tag="VGR29_00495"
     CDS             complement(14640..15605)
                     /locus_tag="VGR29_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV2072094.1"
                     /translation="MDEKQAIAVDLGGTNIRVAVISGDGDIAHRTRVATHAEEGPNAV
                     IERIGTLINQVARMTGLNSDVPVGVASPGPLNPRTGVVHYTPNLPGWRDVPFVEILAD
                     FIAHPATLANDGNCGALGEARFGSANGVQNLVYLALGTGIGGGIISEGVLIDGKNGLG
                     AEVGHITIALDGPRCTCGNVGCLESFASGWAIQRDAEAIATTEDGDLLRKLAGDGEIH
                     AGIVAEAARQGDPAACQVLDRAGRALGAALGTFVNLFDPEMIVIGGGVAALGDLLMGP
                     ARAAIPTHSFVDMRADMRLAYSSLGDDTGLYGAAALAFTVFGDRA"
     gene            complement(15653..17146)
                     /gene="hslU"
                     /locus_tag="VGR29_00500"
     CDS             complement(15653..17146)
                     /gene="hslU"
                     /locus_tag="VGR29_00500"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEV2072095.1"
                     /translation="MVELVRQMTGPDAAMRKPDLGAGEPEPSSLTPKEIVAELDRYII
                     GQEDAKRAVAVALRNRYRRQQLPEELRREVHPRNILMIGSTGVGKTEIARRVARIVDA
                     PFLKVEATKFTEVGYIGRDVESIIRDLVEIAISMLHNERLDAVKEQAARAAVERLAEI
                     MATEAVEQKARRVNKKNGPNPESPEEAVAAAERAAQSTERKRRKEEKRLLELLRQNAM
                     EDETVEIELDAESDMDDGASFEFMPGFSPDDLQDSFMELLDGLLPRRRTRRRMSVREA
                     RRVLAQQEANRMVDVDTVIEEAIQRVEQTSVVFIDELDKTVGESDGGPDVSGEGVQRD
                     LLPIIEGSTVMTRYGPVQTDHILFIAAGAFHNTKPSDLIPELQGRFPIRVELQSLTES
                     DLYAILTEPRNALSRQYLALLETEGVTLDLTEEGLRAVAQLATMVNQRTEDIGARRLQ
                     TIMERVLESVSFDAPDMNGQTIVIDEAYVVDRVGDIAVDDELSNFIL"
     gene            complement(17146..>17382)
                     /locus_tag="VGR29_00505"
     CDS             complement(17146..>17382)
                     /locus_tag="VGR29_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072096.1"
                     /translation="EGDVIEPDDGVVAIGSGAPFATAAAKALITNTDMPAEAIVRESM
                     RVTAEICIFTNDRFTVEVVRVEDDKGGEADEGGA"
BASE COUNT         3705 a         5300 c         4884 g         3493 t
ORIGIN      
        1 cccgacggct tcccgatcaa gggcaacgcc agttccagga ttttccaccc caaggaatca
       61 ccctcgtacg agaacaccgt tgccgagatc tactttgcct ccaacgaggt cgcggagcag
      121 cacgggtatc gcctgccgaa gacaatgcag caggttggag ctactgccgc caatagcgca
      181 gcgggcctcg cccaaaatgc tatggatgca gctggttccg attccgagga gggataaatg
      241 agcctggcaa aggtcacaat catcggcaat ctcggtcgag atccggagac ccgttacacc
      301 ccgtctggcg ccctcaatgt ccaattttcc gtcgccgctt cgcgtagatg gcgagactcc
      361 cagggtcagg agcaggagaa tacgacatgg tttcgggtga ccgcgtgggg tcggctggct
      421 gaaacactcg tgaacctcaa cgagcgcggg gcgctcgtta aggggcgcca agtgtacgtt
      481 gagggccgca tcgagtcccg cgagtatacc gggactgacg gtcaggtgcg gacgtctctc
      541 gacgtcaacg ccaacgaagt ccagttactc ggtgcgcggc aggatggtca gggaggagaa
      601 ggctctagcg gcggtcagca atatggcggc ggctacggtg gcggtcaaca gcgccagcgg
      661 aacgacttcg gcgaccaggg cggatctgac gacctcaacg aggttccgtt ctagtttcac
      721 cccgccacca gtgccattgc ttttagattg aaacgagcga ataacacatg actgacaatc
      781 aacgaccaga gaacgataat cgaccggaac cgcgcgatgc tcgggaaggc acggaaaccc
      841 gggagccgcg agaaggtgga gatcgccgac cgcagccggg tggatcccgc tttggtggcc
      901 cgcagggcca gcgcgacggc ggagagtccg gcggctcccg cttcggcagt tcccagggtc
      961 ctcgtgacgg cggagaaggg gggggatcgc gttttggcgg ttcccaaggt ccccgtgaag
     1021 gcggagaagg cggtggacgc cgtccctttg gagctggtgg cggacgtcgc ccgggcggtg
     1081 gtggaggcgg ccgtttcggt tcgcgtcgca aggtttgcca attctgcacc gataacatcg
     1141 agcatgtgga ttacaaggat atcgggcggc ttcgccgtca cgttggcgag cgcggaaaga
     1201 ttgagccacg ccgcaaactg ggtacctgtg ccaagcacca gcgctccgtc actgtcgcca
     1261 tcaagcgcgc gcgatacatc gggctgctgc cgttcaccaa cagtgctcct cgcggataat
     1321 ccgctgcagt cgatgcacca cggcgcgtct catgggatca catgatccag tggcgcgccg
     1381 ttttccgtaa agttcaacct gacacatcgg gatgcgctca cctgtcctgg acgaggtgtg
     1441 tattcgctag ataaagaagg ccgtagcgtc gatgagtgat cgcttcaaga gaccctccgg
     1501 tacccggact ccgcttggtg accgtggcaa gactcgtctg acatcgccgc cgcgccgtgc
     1561 cagtcgtcgc caaaaggaga cgatgtacca gcggattttc tacatcggat tgatcgccgc
     1621 cggcgtactt atccttgcca ttttgggcat cggggcgttc tgggagtatc aggtcaaacc
     1681 caaccaggta gttgcttcgg tcaacggtga ggaaattacg cgcgaggaat actggaagta
     1741 ccagaacatt gcgctctaca accaggcccg gacctatgaa gactacgcct tgcaggtgac
     1801 gggtcagcaa cagacgcaat tcctgctgta tgccgcgcag ttcgatcaga tgcgggaaga
     1861 ggtgtggggt agcagtgacg tgagcgaggc cacagtccgt cagatgatcg aggatcgtct
     1921 ctatgttgct ggtgccgagg agatgggcat cgatctctca gagccagttc tggaggacta
     1981 tgctctgaat gccttctccc cgccagggca gtcagtagtc actcccattc cgacgcctac
     2041 catgatcccg cagcgcgcgg catgggccac ggagactcag caggcgctgc agacacaaca
     2101 ggcggccgcg ctgggcacgc cgccggcagc cgctggtgca acaccagtgg ggacaccggg
     2161 agcgactccc gccgcaagcc ccgtagcgac cccggatatc gaagccgccc ggatcaccgc
     2221 gagctcagag ttcgagttgt ttcaagagga ggtgttcgat gacgccgaca tttcgctgga
     2281 cgagtacatg gagctctggg tgaagcccca agccgcacgc caactggttg atgcgaagat
     2341 tatcagcgat gttccgcaac gagccgagca ggtccaggcg cagcatatcc tagttgcaac
     2401 cgaggatctt gcgaaccaga cgtacgagca aatcacgagc ggcgcaagtt ttgatgaagt
     2461 cgcgaagacg acctcgacgg atacgatcac tgctccaacc ggtggcaagc ttggatggat
     2521 aaccaaagac cagatgccgg atgcctttac cgaggttgct ttcagtaccg agccgggcga
     2581 ggtagcgggg ccggtgaaaa ctgaatatgg ttggcacatc atcaaggtga acgacaacgc
     2641 agaggaccgt ccgttgacgg aagcacaata ctccctcgca aagacggagg ccgtgagcaa
     2701 ctggattgag cagcagcggg agaagagcga gatttcctcg gactactacg agccgacccc
     2761 tgagccgacg agcgagcagt ttgcaccgcc gcccgatgct cctactccaa ttgttgctac
     2821 cccgattgcc gcgccggatc tgagcgcaac gccggtcgcg gggccgagcc tcaagccaga
     2881 tgcctcgccg gtggggacgc cgatggcaac ccctgggcag taattgatat ctgaaggtgt
     2941 gggtcgtgct ttgacgcgat ccagcggatg atccacactt gacgaagaaa tccccggtgg
     3001 tgtgaagact tgaaaacggc acaccaccat cgccagcaca agtgggagag gagcttgata
     3061 acgtgtcctc acgagacgga tcagaccagt cgggacggaa tcaccgatcg gcaccatctg
     3121 accgtggtgc aaacccacga aagcagtcgc aacgggcaaa tcagcctagc tgggacattg
     3181 atccggcgga gatcgaccgc tatctttcgg ggcgtccctc tcgcgagcag gaaaccaagg
     3241 ctaccgcgcg ttcccgggtt cgtcaggacg caccccaggg aacggctggc cagctcaatc
     3301 ggctgcaacg cgccgtgaac gaccggcgag cctcacacga cgcacgctca gaacccactc
     3361 cgggcgccgc ttcccctggc cgccaacagt ttgccgagcc cgattacgac gacgaagtga
     3421 acgccggaac cagccttacg gagccggatt atggcagcga cgagagctac gaggaaacat
     3481 ctgttcctgc ctcgcctggt cgagaacgct ctccgcgaca ggcccagcgc cggactcagg
     3541 cgagtgccgc ccagcctcct cgtcagcgcc agcaaacacc ccgacagcgg cagcgtccgc
     3601 aggaggagta tgacgatccg agcgacgttg aactctatgc agatgacccc tatctgacat
     3661 acgacgatga gagggactgg gaagtaccga cgcgtcgccg cacggctcgc tctcggccga
     3721 aggtcacatt cacgacgccc agtcttccca aggtatccat gcctcggtct atctcgggcg
     3781 cggaactcgt caacgacatt ccctcgcttg ccttgatggg agcggctatt ctcagcgccg
     3841 ccattatggc tatcgtagtc tctaaccggt tggaccttct cccgcttacg atcccgaccc
     3901 atgtaagcgc aagtggcctc cctgagaaca tcaggggacg gaacgctctc tggagcgtgc
     3961 cacttctgtc cattgccctg tcattgatga atatcgtcac ggcctggttc ctagcgaagg
     4021 ccgacatgtt cgcagcaagg ttcattctcg cggctgccct actcgtccag ttcattgcct
     4081 ggatcgcgct gatccgttac ctctggtagc ggcaagaaat gaaggatgcg aaagagcccg
     4141 gtgctgaaat gcagcgccgg gctctttttg ttccatacgg accgctaccg cgccgctgct
     4201 tgctccaaag cgttacgcgt catcacgttt acgagatgtg cgcggtactc ctcagatgca
     4261 taagtatctc cgttgatctc cagatcaacc gacgccctcg aggccgcgtc tgcgatcgtg
     4321 gttgaagtca ggttcttgcc gttaagggct gcctccaccc ctgttgctct ggtcggcgtg
     4381 gaagtcgcac ccgtaatgac cacgcgagcg tcagacaccg agccatggga catctccagc
     4441 accacagcta ccccgactac agcgtagcca cttgcaggat gcgagtgctt cgcataggcc
     4501 atcttcgtcc ccggagcagg gcgggggatg acgatggtgg tcaggagctc attcggttcc
     4561 agcgaggtgg tccacagatc aacgaagacg tcatctgctg caatctcacg ctccccggaa
     4621 ggacctgttg cgctgatgcg accattcagc gcatgaaaga ccgccgtgag gtccgcggcc
     4681 ggatcagcat gtgcgatcgc cccgcccagc gtgcccctcg cgcgcacctg ctgatcaccc
     4741 acgacattga tagcctcggg caagatcggg aagagcgtcg cgagttccat gtgatcgcga
     4801 atttcgttat acgtcaccat tgcgccgata tgaatgtcag catcgacctt gatcgtgcgc
     4861 agctcggcga tgcgtccaat gtctacgagg gtttcaggag cagcgagtcg caacttcatc
     4921 gccggaatca gcgaatgccc accagcaaga atctttgcat cgggattttg ttccaacacg
     4981 gccacagcat cttcaatgga ttgagcccga tgataggtaa aggacgtcgg aaacactgca
     5041 ttctccccgc aatcgcgaca catgacagat caggaccgcc ccgttaatcc gcagaaaacg
     5101 gttccttctg catcttttcc gctgcgtact gcactgctcg cacgatgttc tgataccccg
     5161 tgcagcgaca caggttcccc tcaagaccat gacgaatctc atcttcggta ggattaggat
     5221 tccgctctag gagagccacg gaggacatga tcatgcctgg ggtgcaatac ccacattgca
     5281 agccatgttt ctcccaaaaa ccctcctgta cagggtgata ggatccggtc cctgacaggc
     5341 cttcgatagt ctgtacattt gatccatcac attggacggc gaacatcgtg catgacttcg
     5401 tggtaatacc gtctacattg acgacacaag cgccacactg gctcgtatca caaccgacat
     5461 gtgttcccgt caggcccagg caatcccgaa ggaaatgcac cagcaaagta cgcggctcaa
     5521 cgtctgcgac gcgttcctgg ccattcaccg tcatcgtcac ccggtggcgg gtgtttactt
     5581 cagtcgagct catcggtttc tccatcccta tcacgtttca cccgctgtgt gaccctgcta
     5641 ttctccatcg atcttgtagc ggggcaactc gtacacggtc attcgaacgg cgaacagaac
     5701 caggtacgcc tctctcccct attctctctg cgcgtgcagt ataccgttga ctgccccttg
     5761 tgtccctcat gatggaaact cttgatcagg acggaccgga gcgtgtagtt tcggaattgt
     5821 cttctcgcat ctcctgaagc atcgcggaga tgatctccgc ggtcatacgc tccagatccg
     5881 gatgtagctc acgggcacgg cgacccagtt gttgaagcac atccgaaaaa ttggacaccg
     5941 gcggcattat tggaccaaag tcctcgatga gctgatcccg gaaccatgag cctatccttc
     6001 ggcgcacctt gttgagtgac ggaggggtaa cacttgcggc taatgccgct ttctcaagct
     6061 gctctctcaa ttcccgattt acgattggaa cggcagtcat cttgttcact aaatctcgct
     6121 gggatcgcac cgactcggcg acggaatccc agcgctggat gacttcccat cctgacacag
     6181 cgtctttcgc cagtccctct gcctcttggc gcacttggtc caggtccagg gttggtttca
     6241 ccttcggcac aagcagcgag cccgtctggt cgtaccagca tccgattccc gccctgacaa
     6301 ggcgaaagta atactgcgcc agcaacacac tctggcggct ttccatgtcg agctggtcaa
     6361 tcccggcaaa caacggtatg agcgacgacc cctctaccgg cttcaagaga ccagtggccg
     6421 tcaggggcag gagcgcaatc tgcgccatat tcacagatag ggaacgatac ccgcgccttc
     6481 gtcccatgac gtagtgcgtg aggctgtaat cgggggcaag aaatattccg tcctcgaccg
     6541 taacgtcgtc gcttacctct cctaccgatt cggtgattat cgggccgatc tccaggagcg
     6601 actggagcga ttccccactg acctgtgaag cccctgctgt ttcgatggaa aattttgcgt
     6661 cgacggaaaa gatgatttgg ccgcgcgcgt cactacccac tacgatgaaa tcgggattct
     6721 gcaacttgtg gcgcgatgcc gtatgcgcga ttgccgggac atcgtcaagc cggacgatac
     6781 ggataacgcg gatctcacgc ccttcgagca cgcggacggt ggtgcccggc caagccgcca
     6841 tcctctcggc gcacacgtcc gaccagcgat ccccaatcag tccgctggcg ccgccacgat
     6901 ctgaaagccg catgatatcg cgatcgaatg tagcggaaag cagcgatctt cccgggaaga
     6961 ccggggattt catggcgagg tcgccttgag atccgaattg ggtcacccgc acccctcaca
     7021 acgaataaag cacaccaaaa tgcagaacct aacaggtatt acctcaggtc gctagggtat
     7081 tgcgtcaacc acggcgacga gcgaagagat gacgaccacg aatcgtgaac cccacggtac
     7141 cggcaacacc ggctatggcg gcaatccggc cgacccaatc caataaggtc actccatagc
     7201 gaagctcgat agatacaggg ccggggggca cattgagttc catatatcca tcatcacggc
     7261 gagttatatc gaccttctca ccgctggcga aagcctccca ccgggggaac caattctgcc
     7321 gaaccaccac ggttccacca ccgtcatcga atctcgcgac aatgtccccg ttgtcgattt
     7381 cggtatcttc tggcatcgag tcaccattac tgacgattga ggtcgggtcg accacgctat
     7441 aaatctccca ttggccgatc gttgtatcga gtgcaagagc cgggcttgtc cttgctgcca
     7501 tacgaggcga gggacccgac ggcactgaca tatcagtaac cacgacgacg ctgataccgt
     7561 tgttgcgcaa gtactcggcg gtcagcgcgt cgcccggatt tgggaaagag tacccgtttc
     7621 ggtagtcata tggaccgcga tggtcccggt gccagtacca caaccagtcg tcgtaataaa
     7681 agggaccagg tgatgcgaca ggcgcccaaa gttgctggtg ccaccagtag gcccggtcct
     7741 cccgattccc tacgacgaag attgatgtgc cctcttcacg ctcggactct gcctcttcaa
     7801 ttgcctccat gaacaaaggg aagtcgtcgt cacctgtggt gtctactggc gtgagtccat
     7861 ggaaattctc ggggacgaag gacgcgggtc gcacaaagat tacaagtgcc gccacggcta
     7921 ctaccataat tacggccgat gaggcgtaag ctcgcttcca taccggtatc tgctgcgaga
     7981 ttttctgaag cccggcacca agggcccacg caccgaaata catcgtgagc tgccgctgat
     8041 agggcatcaa ccgaggagct tccaactgct cgacaagagg tggaacccaa ctgcttgctg
     8101 ctaccgccat cgtgaagagc acgtaggcga ccagtgtgac ggccagtgac tgcgcgacgg
     8161 tgcatggtcg cccgagaatc gcgacacccg tgccaatgat cgccatgatc aacaccggta
     8221 gtgagaccgc cgacattgac gccgtccaga gctcggtgac gggttcatag aactcgtagt
     8281 gaaggaagaa gtattccgcg ttgtagcgga acagtgcgag cacgaccggc atggcaagca
     8341 agagggcgag cacaccgacg agtccaatgc gagaaacggc atcggtaaac cgcgccttag
     8401 cctgctgcct gtcgccggcc gccgtcactg tcgccaaaat ggcgatggcc gcgatcacca
     8461 ctgcaaaaag cgagcgcggg ttcgtacagg caccggccgc tgcagcgagg accgcgagca
     8521 cacccatcgc gtgctcgcgt cgcacgacaa accttgccaa cgccgcgctt gcaagcaacg
     8581 ccagcgaacc tcctgcaacg ttcgtcacca atccccagcc aacgagctcg gtgtagccgc
     8641 cgttcaacca gtgacccggt acagcgaagt gaagagccgt ggcgagcaga gcgacaccgg
     8701 ggtgaatacg gtcgccccta gcgaggaccc agtacgaaac ggccggcaac aggaagactg
     8761 caacgacggc aagcttatgg agcgcgatga tgggcatagt tcccagaaag agcgcccata
     8821 acgcaacatc cagccacgga atcccgagcg gatagaactc gacaggatag ccgccctggt
     8881 gattcccaac ccaacgcagg aggtcgccct ctttcaggga ctcgagcagg agatgagcac
     8941 gggtagcatg aaagggatga tcgacccagg catagacatt agggacttct atctcacgcc
     9001 cctgaatcgt cactatattt gtgagatcga aggccccgat gcgccatgcg gcccacaaac
     9061 acatggcgag aagtaggact aaccaaaccc taccctctac ccgtccggaa tcgtccacgg
     9121 gctgatcacc gggttcaggc atgatccggt ggaactgtct tcgccgggat gtcccgctga
     9181 ggatcagatg ggtccccatc ttcgtggagg aaataccaga gccccagcac agttcctccc
     9241 accatcacgg aaacagagag gtaatacagc gcggtaatga gtgattgagc tggcatctcc
     9301 ggcagatggc gcccgaagta tccccagaca tggacgacac cccgctcgcg aaagtagagc
     9361 tgaccggtgc gccagattcg tgcgtcaacg gagccaaacg cgataaagcc caggaatgaa
     9421 gccgagagca ccaaccagac accaaaacgc gctcgtgagt tctgcgtgat ctcgtcactc
     9481 tgatcggcaa gtcgggagtc gtcggcggac cccggcgaag gagtttgtgc catgtatgtc
     9541 ctgactggga attcccaaaa gattgatggt gccagccggg cattaccact gtccggctgg
     9601 ctcgtcacat caggacttgg cctgtagctc tgcatcggcc acctgtgcag gcgatcgctt
     9661 gatctcgtgg tactcgtcgt cggcattcac gttctcgagg tcatgatgct ctccaagaat
     9721 gttctttgcc gccagcaggc cggtttcgat ggagtgatcg gtgttgttgt atcggaagga
     9781 acccccgcga ccgatgaact gcaagttctc gatcgagcga atgtagtcct tcattttccg
     9841 tagcggctct tcatacccga tgtcatacac ggggtatgct ttggtcgcgc ggagcgtgaa
     9901 gcctccaatg acctcttccg gtttgatgaa gtggagatcc tcgacaagat gcttgaccgt
     9961 ctggttgaca agctgctcgt cactcatgtt ccagatgtga tcgcccttgg tgcaaaagta
    10021 ctccactacc agggaggtga agtcgtcgtt cggcaccatc gccgggctcc agttctttgg
    10081 ctcatggaag cgtccaaaaa gaatgtttcg gtcatggacg taaagccatg tatcgttcgt
    10141 cacctgacgc ttcttgatca tcaggttgac ggtgatgatg tcccggaagg tcaacgagtt
    10201 tgcggcctcg atgacgtcgt ccggtgcagg gggatctgta atcttgcaaa gcacggtcat
    10261 cgggattgaa gagatgaaat tctccgcctc catgatctct atgccgtttt ccgtctggat
    10321 ctcgatgccc tttacgcgat tgccgtcaag gacaatccga cgaacaggat gcttaaggag
    10381 gatctcattc ccgctgtccg tgatggagtc cgccatgcgc tctgagaatc gaccgaagcc
    10441 atcacgcggg tacatgaact cgtcgatcag gctgacgacg gcgcccttgg aaggaatgac
    10501 tgcatccttg attgcagtca tcaacgacat gcccttggtg cgttgcgtta cccagtctcc
    10561 gctgagctga tcgcacggca tcccccagac tttctccgag tagcgctgga agaaaagctc
    10621 atagagtgtc tgcccatact gatcgacata ggcgtcctcc atggagagaa gctccttggg
    10681 gcggaacttc tgctccagtc gtgcgcgccc gtagtcgacc actgccctcg cagccgtgac
    10741 tgggcccacc gacgtcaatg cgcttccaat cttcagcgga tagtcgatga acttgccatc
    10801 gaagtaaatc cggctgatgc ggttcaccca aaggagttcg tcatcgacga cctcctggaa
    10861 caacgcattg agctcatcgt tcttcgtgaa ccagcggtgt ccgccaatgt cgaacctgaa
    10921 ttcaccatac tcggtttggc gtctgatggt ccgggccagt ccacccacaa agggagccga
    10981 ttcgaccaca gtcacgggcg cgccggcctt gctcaggacg taggccgagc atagtccggc
    11041 cgggccggca cccattacca ccgtcgtgcc aacagtgctt tccttcgcac cattgggatt
    11101 gtggccgttc tccatcactt aaggttcctt ccttgggcaa cactggagcc ctggcaagca
    11161 ttgacaacat cgtcggtttt cgaagtcgat tacacgcgtt tcaaccacca cgtcgatgcc
    11221 gcggcgttct cacgcgtatc ctaccatctg ccgcgagggc agtagcggat cccccagtcg
    11281 cgagggatgc cggggagctg cctgtcgcct acaaacgccc gcatcacctg agttatgggc
    11341 cgttccgatt cggcttcagc taggaccgcc aggtaatggc gttattcagg gtgaaattcc
    11401 acaccgcagc gacagccgcg cctatgagat tcgcgagcag gtaatgcata ctcgtgttct
    11461 cgtggaggta cgtcaggata ccccagttga tagcaaggcc accgatatta atggggacgt
    11521 acgtctgggc gcgggagatg atcctgccgg tgccccggtc atgccacgtc catacctcgt
    11581 tcatgtaaaa ggtgactatc atcgagagga aaatcgcgat aggagatgcc acgggaactg
    11641 caagtccgaa cgaggagtga agcaggaaga gcgctccctg gttggttgcg aaccccaccg
    11701 cgccgacgac gagaaacttc tggaaacgct gcataagcgt ccagatcctg attgccggct
    11761 gtggccatga ctcaactgag atcatgcgtt cgcgttactc ctcatgcaga cgcagcggga
    11821 cgtgcgacgg atacatcgcc cagcgtaagc ctggttcctt cacctgtcgt tgactgtcaa
    11881 ctccagggta ttatccaccg gtatggcggt ccgtgactac ctgctggccc tgctccctgg
    11941 attatcttcg gcatacgcac cgagtatgcc atggaagttg gagaggaccc catgcagacg
    12001 agcgaaactc gcgataccgg cactttcgct cgtctgggag ctggagttat taggggatct
    12061 cgagttcttc ccacgcagca tgtagctgcg cgtcctcaat tgcttcgaag gtctcaggcg
    12121 cgatttcttc gtcaccggtc tctgatgtat agatcacagg cagcgcggtg cgggtcccct
    12181 cttccaactc cacggtttcc gttggctcga cgccttccat gtaaatctcg tcgccttcac
    12241 ccgttaacca cagctcggtg ccgagcacgg ccattgagcc atcgctgagc tcgaacggaa
    12301 ggaggacagt tccagtacca aacgtcttct cacccagcac tggagctcgt cctgcctcca
    12361 taagcgagga ggaaacaatc tcggacgccg acgcggagta ctcattcacc agaaccacca
    12421 ggcggccctc ctgatagacc ccattggatc caacggtcct gacgacctcg atgttgccct
    12481 ccgcatcgac ctcctggtaa agcggtgagc cgtccggcag gaactgggag gcaatgccca
    12541 tggcctcaaa aacaagacca ccagggttgt tccgaagatc gagaatgacg ctggttgcgc
    12601 caagcgcctg gccctcgcgt atggcctgct gcaggccttc cgttgcgcca ctggagaact
    12661 gggacacctg gacccacaac acattgttgg gaagcagagc ccatgagacc ggattgacgt
    12721 cgatcttggt acgcgtgatg gtcacctcgt acgtgtccac ctcgccctcg tgctgaagtg
    12781 taagcgtgac gtctgtcccc gcctctcctc gaatacggtt ccctatctcc tcgggctcca
    12841 aaccctctgt cgatacgccg tcgacctcca ggatgacgtc acctggaagg aggccagcct
    12901 caagagcagg agagtccggg ataggtgcga tcacgactgg ggggctggcc tcagtgtcga
    12961 tctggatacc aattcccaca agctcaccgc gggacgattg ttcgaactcc tcggcttctt
    13021 gaggatccag gaaaacggaa tgcccctcat cgccgagggc atcaaccatt ccccgtgcag
    13081 ctccatacat cagttcttcg tcgctgatct cttcctcaag gacgtagttg ttgcgaatcg
    13141 cgtcgtacgt ctcttcaagg atcgtgaact cttcagaggt agtgagttgt gtcgatgcat
    13201 ccacgagacc ggtcgcgatc attgcccgat ccagacccat accgaggcat actgcaagga
    13261 cgagtagaac actcgtcacg attgtccgcg tcctcgacgc ttgaccgctc ctcgcttcgc
    13321 gagattccga tggattcatt ggtcctccag aagccgttgc ctggctggta agaagaagaa
    13381 agagatcttg ccggcatgtg cggcttggac tgacaggaag gtagaaccga ctcctcgatc
    13441 cgcattcccg gcaataatga tgcatttcga agctgaatcc atgctgaatc ggcgagcatc
    13501 acgcggaggg aaatcgttcc gcccgcacat tgtacgcgtt tcaaagatgt gattatcccc
    13561 gggacaacgt tcgccgttcc ggggattatt cgctacgcac ttgatggtcc agggtcgtta
    13621 cgcggatgcg ccgaccgcgg tccgccaact ctctcgtttt gcttcattgc ctcgaactgc
    13681 ctccgtgaac agccgctcca gttgatgcgt cacggggcca ataccaccat cccctattag
    13741 gaagcggtcg atctcaacga ttggcgtaat ctccgccatg gtcccgcata gaaagatctc
    13801 atctgcggta tacagctccg tccggtcaac ccggcgttcg atcaccgtca ggccgagatc
    13861 ttcacgtgcc aacgtcatga tgctatcgcg ggtaatgctc tcaagtatgc cactggccgg
    13921 gtcgggagta ataagctgat cgttcttcat gaacatgacg caggctcccg gtgcttcggc
    13981 gaccttgcca tccggtccaa gcagcagcgc aacatcgtac ccgtccagtt tcacctcata
    14041 ggtcgccagc tggccgttac ggtaatttga aatgttcttc acccgtggcg gcattacatt
    14101 gtctgagatc cgcgtccagc tgctcgtctt ggcgcgctgc ttcacccccg taccgaggtg
    14161 cgagggcctc ggatcggtgg tgatgaggag atcgccgcgg agcttcgctg gatcagatcg
    14221 tcctgtccga gcgccgccaa agtacaccag cgggaagata taagtgtctt cacgactccc
    14281 gttcctccga agcaagtcca cggtattttc gatcaggtcg tcgaccgtaa catcgatcgg
    14341 cagccgcacc attttcgaag aatcttttag gcgccgcatg tgctcgtgga gtcgaaaaac
    14401 gtagagctct tcctgctccc cattccagta cgcccgaatg ccctcgaaaa ctgcccccgt
    14461 tgccgaccag gcaatgtcag agacgtggac tgtggcatca tgccaggcaa ccggctcacc
    14521 gttccaccac aaatattttg gatgctggct cattccctgt tcctcggctc atacggagtg
    14581 acggtataac ggcgccattg tcgcacgggc ccatgagcgt gcctacattc gtgccgtaat
    14641 caggcccggt ccccaaaaac cgtgaatgcc agcgctgcgg ctccgtacaa acccgtgtca
    14701 tcacccaagg aggaatatgc cagcctcata tccgctcgca tatcgacaaa ggagtgtgtc
    14761 ggtatcgctg ccctcgccgg tcccataagg agatccccca aagcggcgac tccgcctccg
    14821 atgacgatca tttcggggtc gaagaggttg acgaaagtcc ccaaagcggc acccagtgcc
    14881 cgtcctgccc tgtcgagcac ctggcacgcg gctgggtcgc cttggcgcgc ggcttcggcg
    14941 acgatgcccg catgaatttc cccatcgccg gctagcttgc ggagcaggtc cccatcttct
    15001 gtcgtggcaa ttgcttcagc atcgcgctga atcgcccacc ccgacgcaaa tgactcgagg
    15061 cagccgacat ttccgcaggt acagcgcggg ccatcgaggg caatggtgat gtgcccgacc
    15121 tctgccccta aaccgttctt gccgtcgatc agtacgccct cggaaataat gcccccgccg
    15181 atcccggtgc caagagcaag atacaccaga ttctgcacac cattcgcact gccgaaacgt
    15241 gcttcgccga gggcgccgca gttcccatca tttgccaggg tggcgggatg agcgatgaag
    15301 tcggcgagga tttctacgaa gggcacgtcc cgccaacccg ggagattcgg ggtgtaatgc
    15361 acaacaccgg tgcgtggatt gagcggacca ggagaagcaa caccaacagg aacgtcgctg
    15421 ttgagaccgg tcatcctggc gacttggttg atcagtgtcc cgatcctctc gatgaccgca
    15481 ttgggtccct cctcggcatg agtggccacg cgggttcggt gagctatgtc gccatcgcca
    15541 gaaatgaccg ctacccgaat gttggtacct cccaggtcaa ccgcaatcgc ctgcttctcg
    15601 tccacgaaac ctcctgcttt ctggataagc gtccccggcc gacctgagta gatcagagga
    15661 tgaagttgct cagctcatcg tcaacggcaa tatcaccgac gcggtcaact acgtatgcct
    15721 catcaataac gatggtttgc ccgttcatgt ccggcgcatc gaacgagaca ctttccagca
    15781 cccgctccat gatcgtctga agtcgccggg cgcctatgtc ttccgtccgt tgattcacca
    15841 tcgttgcaag ctgggccacc gctcgcaaac cctcctctgt caggtccagc gtaacgccct
    15901 ctgtctcgag cagggctaga tactgacggg agagcgcatt ccggggctca gtgagtatgg
    15961 cgtagaggtc actctcggta agcgactgca actcaacccg gatcgggaat cggccctgga
    16021 gttccgggat caggtccgat ggcttcgtat tgtggaaggc gcccgctgcg atgaagagga
    16081 tatgatcagt ctgtactgga ccatagcggg tcatgactgt ggaaccttcg atgatcggca
    16141 gcaggtcgcg ctgaaccccc tcgccggaca cgtccggacc accgtcgctt tcacccaccg
    16201 tcttgtcaag ttcatcgatg aagacgactg aggtttgctc gacacgctgg atcgcttcct
    16261 cgatgacggt gtcgacatcc accatccggt tcgcttcctg ctgtgcgagg acccggcggg
    16321 cctcccggac cgacatgcgg cggcgggtcc tgcgccgcgg cagcagaccg tcgagaagtt
    16381 ccatgaagga gtcctgcagg tcgtcgggag aaaatccggg catgaattcg aaggaagcac
    16441 cgtcgtccat gtcagactct gcatccaact cgatctcgac ggtctcgtct tccatggcgt
    16501 tctggcgaag gagctcaaga aggcgctttt cttccttacg tcgctttcgc tcggttgact
    16561 gagcggcccg ctctgccgcg gcgacggctt cctcgggact ctcgggattt ggaccgtttt
    16621 tcttgttcac ccggcgagct ttctgctcca ccgcctctgt tgccataatc tcggccaggc
    16681 gctcgacggc agcccgcgca gcttgctcct tcacggcatc gaggcgctca ttgtgcagca
    16741 tggaaatagc aatctcgacc aggtcacgaa taatggattc gacgtcacgg ccgatgtaac
    16801 caacctcggt gaacttggtg gcctccacct tcaggaaggg cgcgtccacg atccgtgcta
    16861 cccgacgagc gatttcggtc ttgccgacac cagtggaccc gatcataaga atgttgcggg
    16921 gatggacctc gcggcgaagc tcctcgggca actgctgacg acgatagcgg ttccgtagcg
    16981 cgacggcgac agctcgcttc gcgtcctcct ggccaatgat gtaccggtcc aactctgcaa
    17041 cgatctcctt tggcgtgaga ctgctcggct caggttcccc tgctcccaaa tccggctttc
    17101 tcattgcggc atcgggcccc gtcatttggc gcacaagttc gaccatcacg cccctccctc
    17161 atcggcttcc ccgcccttgt cgtcctcgac gcgaacgacc tcgacggtga acctgtcgtt
    17221 cgtgaagatg cagatttctg ccgtcacccg catcgattcg cgaacgatcg cttcagccgg
    17281 catatccgtg ttcgtgatca gggccttcgc cgcggcagtg gcgaagggag caccagagcc
    17341 aatagcaacg actccatcgt ccggctcaat cacatcaccc tc
//
LOCUS       DASYWM010000012         7238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_300724, whole genome shotgun sequence.
ACCESSION   DASYWM010000012 DASYWM010000000
VERSION     DASYWM010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300724"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VGR29_00510"
     CDS             <1..619
                     /locus_tag="VGR29_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072097.1"
                     /translation="LSLDEAAELQLVTRRGLAVERETYQHLSDVGLLPPTATRVLLHE
                     VDDQIEALGVGRVTLAEVYNRERPGFDRIVERITGWLPNPIGDDPTTLAYAEASARRL
                     AARRTSEALEMFTRLPNIGQVVIDEARATFARWEEEAVTQLSELDADVTQDMREIHQQ
                     QAETLIRVASTDALEELAEIGILPEALARRAAETVATEVESDRGV"
     gene            923..1330
                     /locus_tag="VGR29_00515"
     CDS             923..1330
                     /locus_tag="VGR29_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072098.1"
                     /translation="MHIEVPRIVGTTADDVLAKLIAWELLARSLDGLQETAADFAALL
                     QNELGDVDDDSARQQRRFCLAVEYAATSIARDHHDTSQESTDPLVIEARSSDFLRVLE
                     HASEALRLADEALGIEQGQGAQGPPDATPPMMP"
     gene            complement(1327..1656)
                     /locus_tag="VGR29_00520"
     CDS             complement(1327..1656)
                     /locus_tag="VGR29_00520"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEV2072099.1"
                     /translation="MLFYCEFTWFPGTTREQVARRLVQQHDAGSNHPDRIRGWYNLAG
                     GGAGFLIVEYDDIRQLTAFLQPYMELMSFDVRAIEQLDYEDRLSALRSLVGQSSPEDT
                     PARMDYA"
     gene            2041..2409
                     /locus_tag="VGR29_00525"
     CDS             2041..2409
                     /locus_tag="VGR29_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV2072100.1"
                     /translation="MPKDDLSGKKLDHISLSGANLSDSDLSKSSLRRSHFEGASLQDA
                     NLAESDLQGAHLNHADVTGAILRNADLTGADLRGVDLGRAASTEGAKLTDAKGASLEQ
                     AEQAAEDRVISVEWADDADE"
     gene            2470..2739
                     /locus_tag="VGR29_00530"
     CDS             2470..2739
                     /locus_tag="VGR29_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072101.1"
                     /translation="MVEAAKIGPLPSLQKGRTVNVSCARADVLDMIGTIVEATPFVEK
                     ARTRASMGSRIATGILAEEGPPAAHGHLSLLGVGGSSVEHLLACW"
     gene            2803..3648
                     /locus_tag="VGR29_00535"
     CDS             2803..3648
                     /locus_tag="VGR29_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEV2072102.1"
                     /translation="MKRTFLSLFVVLMLFSPVFGVTAQEVPSPVPEATAEAARVGESG
                     PGFGREGQRPGTAEFVEMVIQDVNLTWANLFQSWGYSYIPPTYVTIEAGAYARSSCGI
                     NAGNPMEDPSLNPALYCIYGGELGTQAIGSPDVFETQVVYSPVIYLSIPWLEESSSTN
                     ASNADFTVAYRVAHEVAHHIEYLLGYIDHTGGGCCGYTDEQIDLWADCLTGVWAYSAY
                     DEGKLAEPDVKGAQAAAWGDEADLPEEFGREADHGNPEQRLLSLMTGYENGNASLCIE
                     GDPGG"
     gene            complement(3741..4022)
                     /locus_tag="VGR29_00540"
     CDS             complement(3741..4022)
                     /locus_tag="VGR29_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072103.1"
                     /translation="MIPRPQHQHLDPETDEDTSLMSRRAMLRRSLLGITGAGLASLLV
                     ACGGDEEDAEDDEAGDVQPPDAPDVDPDEPALPGTDDEEDQDDQAEEDD"
     gene            4301..5530
                     /locus_tag="VGR29_00545"
     CDS             4301..5530
                     /locus_tag="VGR29_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2072104.1"
                     /translation="MTTAQVNTSYKSLFEPSGAVVFFLATFIGRVPLAMKALGCVLLV
                     EILTGSYGLAGAVGATQTLVSAFASPRLGRFADRYGERKILFWSIFVHTVGMAWLIIA
                     AYTAAHPALLLTGAALIGGSTMPFGSLSRARWVHTLGQGRRLEKAYALESMADEAGFI
                     IGPLLVIPLSVSVNAAAGLLASLVMTIAATIILIIQNAPRTNVRISPQENDVPEGKTV
                     IGIPGIQVLIGSLLFMGIIFGAVEILLVAFAEHLGQPNAASIMAALFALGSLIGAVGY
                     GAIQWHSPVDRRLKIAIWWIALGTIPILWANTVLLMSLAVFVTGLAISPGMIAANTVV
                     EHLAPPKMLTEAFSWIGSALATGAAFGSIIVGIVLDEFGVRGGQAVGLVGGLLSALVV
                     IVWAKHLKVDPPPEPGF"
     gene            5685..6617
                     /locus_tag="VGR29_00550"
     CDS             5685..6617
                     /locus_tag="VGR29_00550"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEV2072105.1"
                     /translation="MAFDITLDYAFFEGKIVPFSEAKVSIGTHALQYGTGAFAGIRGY
                     RSEDGETINIFRLRDHCARLLRSARLLRMDLPFDVDSLAETIVELTKKNAPTSDVYYR
                     PFVYKASVQLSPRLQGIDDELAIYMLPMGDYLDTTRGQSAIVSSWTRASDNAIPSRGK
                     ITGSYINSSLAKDQAMVYGADDAIMLNADGKISEGSSCNLFIVRDGVLITPPINSDIL
                     EGITRRTVMQLAEEMNIPVEQRAIDRSELYIVDEAIFCGTGVQLAWIEVIDGRTIGDG
                     AQGPIARQLQERLFDIFRGRTGDHKEWLTPVAIS"
     gene            6981..>7238
                     /locus_tag="VGR29_00555"
     CDS             6981..>7238
                     /locus_tag="VGR29_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072106.1"
                     /translation="MFPPIEPFATGMLPVDDDNVVYWEASGNPEGKPALHLHGGPGGG
                     MMAGYRRRFDPDRYMIVGFEQRGCGRSRPLATDADLSTNTTQ"
BASE COUNT         1410 a         2232 c         2167 g         1429 t
ORIGIN      
        1 cctctcactc gacgaggcag ccgaactcca gctggtgacg cgccgggggc tcgcggtgga
       61 acgggagacc tatcagcacc tgagcgatgt gggcctgcta ccaccaacgg ctacgcgcgt
      121 gttgctgcat gaggtcgatg accagatcga agcgctaggt gtgggtcgtg tcacgcttgc
      181 cgaggtgtac aatcgtgaac gaccaggttt cgaccgaata gtagagagaa ttacgggctg
      241 gttgcccaac ccgatcggcg atgatccgac cacgctggcc tatgcggagg ccagcgccag
      301 gcggctggcg gcacgacgta ccagcgaagc cctggaaatg ttcacacgcc taccgaacat
      361 cggacaggtc gtcatcgacg aggccagggc aaccttcgcg cgatgggaag aggaagctgt
      421 gacccaactc tccgagcttg acgccgatgt gacacaggac atgcgcgaga tacatcagca
      481 gcaggcagag acgctgattc gcgtggcatc gacggatgcc ctggaagaac tcgctgagat
      541 cggcatattg cctgaagcct tggcgcgtcg agcggccgaa acggttgcga ccgaggtcga
      601 gagcgaccgg ggtgtgtgac tcgactgtct cctggctcca tggggtgatg atcggccatg
      661 tggatttccg gattcgagtc taaggggaga ggctggagcg tccatccgtc aattggggaa
      721 ttcgcggagg agaggttacg acaactaccg aaggatccgc agttctcacg tcggtcacgg
      781 cgctctagga caggcactga cgcctgaagc aagagcaagc tccatcggca tctcgtttct
      841 catgaatgac aatgccacgg atggctcaaa acgtgccatg agggagtacg acggctgaat
      901 cacgatgaaa ggaccctgcg gcgtgcacat tgaggtaccg aggatcgttg gcacaacggc
      961 ggacgatgtc ctggccaagc tcattgcgtg ggagctgctc gcccgctccc tggatggact
     1021 ccaggaaaca gcagccgatt tcgcggccct gctgcagaac gagttaggtg atgtcgacga
     1081 tgactccgcg cggcagcagc gaaggttctg tctcgccgta gaatatgcgg caaccagcat
     1141 cgcccgtgac catcacgaca ccagccagga gagtaccgat ccacttgtga tcgaggcgcg
     1201 gagcagcgac ttcctgcgtg ttctggagca cgcatcggag gcactgagat tggccgatga
     1261 agcactcggg atagagcaag gacaaggcgc tcagggtcca ccagatgcga ccccgcccat
     1321 gatgccttag gcgtaatcca tccgcgccgg agtatcttcg ggggatgact gcccgacgag
     1381 agaacgcagg gcgctgagcc gatcctcgta gtccagttgc tctatcgccc gcacgtcaaa
     1441 gctcatcaat tccatgtacg gctggagaaa cgccgtcagt tgtcgaatat cgtcgtactc
     1501 gacgatcagg aagcccgcac caccacctgc caggttgtac cagcccctga tcctgtcagg
     1561 atggttcgag cctgcatcgt gctgctgcac cagccgccgg gctacttgct cccgggtggt
     1621 gccagggaac caggtgaact cacaatagaa caacatcggc tcctcctttt ggttgtgacg
     1681 cgcgtgtctc tgtattgtac gccggcagtc aatgatgcag tcatgccccg gcccaggagt
     1741 acacaggttg gcaactgaat cagttcctac cccgccccag gaacaccagg cagcgtatca
     1801 gagcccgcgt gacgacctcc tgcctcaggt ccatactcct gtacggtcca cctttggccc
     1861 aagggaaggg actggcgcgc ccatgacctt gacctccttt ttctccctgg agtcatagca
     1921 gactggggac gccgtcgata tactacgcag agacactgtt ttcagatcat cacgtcatac
     1981 atcacctctc cccgggtgag gctcaagcat tctctctatc actcctgaaa ggagccacct
     2041 gtgcccaagg acgatctctc aggtaagaag ctggaccaca tcagcttgtc tggtgcgaac
     2101 ctctcggatt cggatctctc gaaatcctcg ttgcgccgct cacacttcga aggcgcctcg
     2161 ttgcaagacg ccaacctggc cgagagcgac ctgcaaggag cccacctgaa tcatgcggac
     2221 gtgaccgggg cgatcctgcg caatgccgat ctcaccggcg ccgatcttcg cggggtcgat
     2281 cttggccgtg cagcatcgac cgagggagcc aaactcacag atgccaaggg cgcgtccttg
     2341 gagcaagcgg agcaggcagc ggaggaccgg gtcatcagtg tggagtgggc ggacgatgcg
     2401 gacgagtaag ggcgcctgat ccactctcgt gatcgaacat cttctgtttg cgttggcacg
     2461 cccgcctgaa tggtagaggc ggccaagatt gggccactcc cgtccctcca aaagggccgc
     2521 acagtaaatg tctcgtgcgc acgggccgat gtgctcgata tgatcggcac tattgtcgag
     2581 gcaacgccct tcgtcgagaa agcacggacg cgagcctcga tggggtctcg gattgcgacc
     2641 ggcatactcg ctgaggaggg accgccagcc gcccatggac atctcagcct gctgggtgtt
     2701 ggaggctcgt cagtcgagca cctgctcgcc tgctggtgaa ggagcaccga tcagatttcc
     2761 atatgaaaat catgtttcgt tgaatcccaa gggaggtcac acatgaagcg gacgttcttg
     2821 tcattgttcg tcgttttgat gctcttctcc cctgtctttg gagtgacggc acaagaggtt
     2881 ccgtccccag tgccggaggc gacggcagag gcagctcgtg tcggcgagtc cggtcctggc
     2941 tttgggagag aaggacaacg gccaggaacg gccgaattcg tggagatggt gattcaggat
     3001 gtcaacctca cgtgggcgaa tctcttccag tcctggggat actcctacat tccgccaacc
     3061 tacgtaacga tcgaagctgg tgcctacgcc aggtcaagct gcggcatcaa cgccggcaac
     3121 ccgatggagg acccctcgct caatccagcg ctttactgca tctacggagg tgaactgggt
     3181 acacaagcga ttgggtcacc ggacgttttt gagacccagg tcgtgtattc ccccgttatc
     3241 tatctctcca ttccatggct ggaggagtcc tcctcgacaa acgcgtcgaa tgctgatttc
     3301 actgtcgcct accgggtggc gcatgaagtt gcccatcaca tcgaatatct cctcgggtac
     3361 atcgatcaca cggggggagg ctgctgcggc tacaccgatg agcagatcga tctatgggct
     3421 gactgcctta ctggagtctg ggcatactcg gcctatgacg agggcaagct cgcggagccg
     3481 gatgtcaaag gggcgcaagc tgctgcctgg ggtgacgaag ctgaccttcc agaggagttc
     3541 ggtcgcgaag cagaccacgg caatccggag caacgcctgc tatcgcttat gactgggtac
     3601 gagaatggaa atgccagcct ctgcattgaa ggcgacccag gagggtgagg ccgagagata
     3661 ggcctgctga ttgagccacc gggactcgat ccaatctgtg acgcgggctc tcggggccag
     3721 gggagccgtt ctcgcgtctg tcagtcgtcc tcctccgcct ggtcatcctg atcctcctca
     3781 tcgtcggtcc cgggaagagc cggctcatca ggatcaacgt cgggtgcgtc tggcggctgg
     3841 acatcaccag cctcgtcgtc ctccgcatcc tcctcgtcac cgccgcacgc cacgagcagg
     3901 gacgccaaac cagcgcccgt gatcccaagc agggatcgac gcaacatcgc gcgacgggac
     3961 atcaagctcg tgtcctcgtc tgtctcaggg tcgaggtgct ggtgttgagg gcgcgggatc
     4021 atactgtcga ctccttccga aacataagac tccccagcgt agatactggt gggctatgtc
     4081 gcgttggtgg caagaaacgc accactggga gcggttcaag tcgttgtcgg gcccctggtg
     4141 ttggatcacg tgaggtatcg gtgccaacgg aggctgcagc aacaccatcg gccttatcct
     4201 cgattccgtg cgtccggata tcgtatcgag taccgtaggc aacgtgcctg gtccgcttac
     4261 cggcacattc tttttgcgcc cagcttcgga tctgcctttc atgacgaccg ctcaggtgaa
     4321 taccagctac aagagtctct tcgagccgtc gggtgccgtc gtcttcttcc tggcaacctt
     4381 catcggccgc gttccactgg ccatgaaggc gctcgggtgt gttctcctcg tcgagattct
     4441 caccggttcc tacggcctcg caggggcggt cggcgcaacc cagacgctcg tttccgcatt
     4501 cgcttcacca cgactgggcc gcttcgccga tcggtacggc gagcgaaaga tcctcttctg
     4561 gagtatcttc gttcacacag taggcatggc gtggctcatc atcgccgcgt acacggcggc
     4621 tcaccccgct cttctcctga ccggcgctgc cctcatcggc ggatctacca tgcccttcgg
     4681 gtcactcagc cgggcacgct gggtgcacac gcttggccag ggccgacgcc tggagaaggc
     4741 atacgcgctg gagagcatgg ccgacgaggc gggtttcatt atcggtcccc tgctggtgat
     4801 tcccctctcg gtcagtgtca acgcggctgc tggcctgctc gcctcgctcg tcatgacgat
     4861 tgccgcgacg atcatcctca ttatccagaa cgctccccgc accaacgtgc gcatctcccc
     4921 gcaagaaaat gacgttcccg agggcaagac cgtgatcggc atccccggga tccaggtgct
     4981 gatcggctcc ctgctcttca tgggcatcat cttcggagcg gtggagatcc tccttgtcgc
     5041 ctttgccgag catcttggcc agccaaacgc cgcgagcatc atggcagcgc tcttcgccct
     5101 tggctccctc atcggcgcgg tcgggtacgg ggcgatccaa tggcattcac cagtggaccg
     5161 gcggctgaag atcgcgatct ggtggatcgc ccttggcacc attccgattc tctgggcaaa
     5221 cacagtcctg ttgatgtcgc tggcagtctt tgtcaccggg ctggcgatct cccccggcat
     5281 gatcgccgcc aacacggtgg ttgagcacct tgcgccaccg aagatgctga cggaagcgtt
     5341 ttcctggatc ggctccgccc tcgcgacggg cgccgcgttc gggtccatca tcgttggcat
     5401 cgtgctggat gagttcggcg ttcgcggcgg tcaagccgtg ggcctcgtcg gcggcctgct
     5461 ctccgcgctt gttgtgattg tgtgggcgaa gcatctaaag gtggatcccc cgcctgaacc
     5521 cggattctga cgggcctgat cctcccgaat cctgtgcgat actgacgcct gcgtacgcat
     5581 gcctcgtcgc cgcgttcctg atgcatgggg acgccgatgt agttgccggc aatatccgat
     5641 atgacggggc aagaccgccc acagcagatc ctggagcacc caacatggcg ttcgatatca
     5701 cgctcgatta cgcattcttc gaagggaaga tcgttccgtt ctccgaggcg aaggtcagca
     5761 tcggcacgca cgccctgcaa tatggcacgg gtgccttcgc cggcattcgg ggctaccgct
     5821 cggaagacgg cgagacgatc aatatcttcc gcttgcgtga ccactgcgcc cgcctgcttc
     5881 gctcggcccg gctgctacgg atggaccttc ccttcgacgt ggattcgctt gcagagacga
     5941 tcgttgagct gacgaaaaag aacgccccga cctcggacgt ctactaccga cccttcgtct
     6001 acaaggcatc cgtccagctc tccccccgcc tgcagggcat cgatgacgaa ctggcgatct
     6061 atatgctgcc gatgggcgac tatctggaca ccacgcgcgg ccagagcgcg atcgtctcct
     6121 catggacgcg tgcctccgac aatgccatcc cgagccgggg caagatcaca ggctcctaca
     6181 tcaactcctc gctggccaag gaccaggcca tggtctatgg cgccgacgat gcgatcatgc
     6241 tcaatgccga cgggaagatc tccgaaggaa gctcctgcaa tctcttcatc gtgcgggacg
     6301 gcgtgctgat caccccgccg atcaacagcg acattctgga gggcatcacc cgccggaccg
     6361 tgatgcagct ggccgaggag atgaacatcc ctgtggagca gcgggcgatc gaccggtcgg
     6421 aactctacat cgtggacgag gcgatcttct gcggcaccgg cgtgcaactg gcatggatcg
     6481 aagtcatcga cggccgcacg atcggcgatg gcgcccaagg tcccattgca aggcagctgc
     6541 aagagcggct ctttgacatc ttccggggcc gcacgggaga ccacaaggaa tggctcaccc
     6601 cggtagccat ctcctaacgt ggtttgcaga atcattgacc gttatccctg tagcggacga
     6661 cattgctgcg gcaaggggcc gctcccgctc aattgcttcg caattgccta tgcgtcgtgc
     6721 gctacaggca atcaacgtga aaacatccgg caacctctct gcgaaccact ttagcggcga
     6781 gtcgtaacgg cttgcgggag gccctcgtga attcccctgc cccccatgac gagttgcgac
     6841 atttcttcag gcgcatttcg cgcccgtacg ggccggggcc tcattatttc gtagcggcat
     6901 cccacaccgt cacgattggc gaaggaaccc ccttggtagg cccttatggt ctcctctact
     6961 gctgatgaca ggatgcggca atgtttccac cgattgaacc gttcgcaact gggatgctgc
     7021 cggtagatga cgacaatgtc gtctactggg aggcgtccgg gaatccggag ggcaagcccg
     7081 ccctgcacct gcacggggga ccgggtggcg gcatgatggc gggatatcgc cggcgcttcg
     7141 atcccgaccg gtacatgatc gtcggcttcg agcagcgagg atgcggcagg agccggccac
     7201 tggcgaccga cgccgacctt tccaccaaca ccactcag
//
LOCUS       DASYWM010000013         3667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_432080, whole genome shotgun sequence.
ACCESSION   DASYWM010000013 DASYWM010000000
VERSION     DASYWM010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432080"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VGR29_00560"
     CDS             <1..940
                     /locus_tag="VGR29_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2072107.1"
                     /translation="LGLAGESGCGKSTIAQAILRILQPPAVITGGQVLFQGRDVLEMS
                     DDQLEKFRWDQVSMVFQSAMNSLNPVMTIGDQIIDAIKAHNPRTSDRQAQERAAEMLR
                     IVGIEETRIDSYPHQLSGGMRQRAVIAIALVLDPPLMIMDEPTTALDVVVQREILAQI
                     ADLRERLGFSIIFITHDLSLLVEISDRIAIMYAGQIVEMANARELFDNPLHPYTQGLM
                     SSFPSLTGDRVPLTGIPGSPPNLIDPPSGCRFHPRCPKRMLICDDPVPLLGEARPGHW
                     VACHLYEASRPDKYGPSQLEPHPQAMAAHNAERNIL"
     gene            937..1953
                     /locus_tag="VGR29_00565"
     CDS             937..1953
                     /locus_tag="VGR29_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2072108.1"
                     /translation="MTETLTKQDLVMDIRNLSKRFPVGGLFDSRKVHAVEDVSFQMPR
                     REIVTLVGESGSGKSTTARLLARLIEPTSGEIIYDGKDVLKTEPNGASLKYRGDVQMV
                     FQDPFGSLNPAHPIEYLLERPLKIHKKVRGRRDTREKIIELLETVGLTPAAEVAAKFP
                     YELSGGQRQRVAFARALSVEPKVMLADEPVSMLDVSIRVGVLNLMRRLTEERGLSFLY
                     ITHDIASARYIGDKTIVMYAGRMMEGAESRELIEKPAHPYTKLLLSAVPNPHAGLTTR
                     EAPTRGEIPSLVDPPPGCPFAARCPDVMPICREQISPPVIISPSHWVRCHMYDGQTMD
                     TAAL"
     gene            2063..3193
                     /locus_tag="VGR29_00570"
     CDS             2063..3193
                     /locus_tag="VGR29_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2072109.1"
                     /translation="MIKVGIIGCGDVATHSYMPGLNRFNDRAKVVALFDIVEERIQNA
                     KAFHPNATGYTDYGEFLSHSDERMDLVLNLTPAPLHREITSRALEAGYNVYSEKPVAS
                     TLEDAQELVQIADSRGLTFFCAPAMMVTSRMMWLKQQMEEGTFGRAYFLKCQIGSMGP
                     AAWREYSGDPRVFYTPKVGPLIDLGVYMLHTMTGFFGPAKRIQAVGGIMNEERVMLQP
                     ARFGEKLKVEVPDLYSINIEFENNRYGHLFNSFAVPRSKAPMFELYGSMGTASVGLPE
                     WYDANGTTDLYLREESASGDNEFWRDGVPVPNPSRAVGVLDSGLLHAFDVLEYGVPNI
                     LTAAHATHVLEIMLACGTALETGESIEITSTFEEEARAVTTA"
     gene            3207..>3667
                     /locus_tag="VGR29_00575"
     CDS             3207..>3667
                     /locus_tag="VGR29_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072110.1"
                     /translation="MKREQIGVQMYTLREMAAKNLDATLGEIARIGYAGVEIAGFYGH
                     SASDVKSMLDRYGLKAFSAHIPAAQFEGDIESVVSDLTTIGAPWGIVPWVSPEERNET
                     AMRDLGLKMNAYASHLKDADIKFGYHNHDFEFTEKVGTGETLFHTLLNITDP"
BASE COUNT          762 a         1042 c         1132 g          731 t
ORIGIN      
        1 actggggctg gccggcgagt ccggttgcgg aaagtcaact attgcccagg caatcctgcg
       61 catcctccag ccaccggcgg ttattaccgg cgggcaggtg ctcttccagg ggagagatgt
      121 ccttgagatg agcgacgacc aactggagaa gtttcgctgg gatcaggtct cgatggtctt
      181 ccagagtgcg atgaattcgc tcaatcctgt gatgacgatc ggcgaccaga tcatcgatgc
      241 gatcaaggcc cacaatccca ggaccagcga ccggcaggcg caggagcggg ctgcagagat
      301 gctccgcatt gtcgggatcg aggagacgcg gatcgacagc tatccgcatc aactctctgg
      361 cggtatgcga caacgcgcgg tcattgcgat tgccctggtg cttgatccgc ccctgatgat
      421 catggacgag cccactaccg cactggatgt ggtggtgcag cgggagatcc tggcgcagat
      481 cgcagatctc cgcgagcggc tcgggttctc gatcatcttc atcacgcacg atctctcgct
      541 gttggtggag atttcggacc gcatcgccat catgtatgcc ggtcaaatcg tcgagatggc
      601 gaacgctcgc gagctgttcg acaatccgct gcatccctat acccaggggt tgatgagctc
      661 gtttccctcc ctgacgggtg atcgggtacc gcttacggga atccctggtt cgccgcccaa
      721 cctgatcgac ccgccatccg gctgccggtt ccatccacgg tgccccaagc ggatgttgat
      781 ctgtgacgat cctgtgccat tgctcgggga agcccggccg ggccattggg tggcatgcca
      841 tctctatgag gcgtctcgac cggacaaata cggtccatcc cagctcgaac cgcatccaca
      901 ggccatggcg gcccacaatg cggaaaggaa cattttatga cggagacact gacgaaacag
      961 gatctggtga tggatatccg gaacctcagc aagaggttcc ccgtcggcgg actgttcgat
     1021 tcccgcaagg tgcacgcggt tgaggatgtg tcgttccaga tgccccgccg ggagattgtg
     1081 acgctcgtcg gggaatcggg ttccggcaag agcacgacag cccgcttgct ggctcgactg
     1141 atcgagccga catcggggga gatcatctat gacggcaagg atgtgctgaa gaccgagcca
     1201 aacggtgcgt cactcaagta ccgtggcgat gtccaaatgg tcttccagga tcccttcgga
     1261 tcgctcaatc cggcgcatcc gatcgaatac ctgctcgagc ggccattgaa gatccacaag
     1321 aaagtgcgag ggcgaaggga cacgcgcgag aagatcatcg aattgctcga gacggtcggg
     1381 ttgaccccgg ccgccgaggt ggccgcaaag ttcccgtatg agctctcagg cgggcagcgc
     1441 cagcgtgtgg cattcgcccg tgcattgtcc gttgagccga aggtaatgct ggccgacgag
     1501 ccagtgtcga tgctcgacgt gtcgatccgc gttggggtac tgaatctgat gcggcgactc
     1561 actgaagagc ggggcctctc atttctctat atcacgcacg acatcgccag tgcccgctat
     1621 atcggcgaca agacgatcgt gatgtacgct ggccggatga tggagggcgc cgagagccgc
     1681 gagctgatcg agaagccggc gcatccctat acgaagctgc tcctttctgc ggtgccgaac
     1741 ccgcatgccg ggttgacgac gcgcgaggcg ccgacacgtg gcgagattcc ctcgctggtc
     1801 gatccgccgc cgggttgccc gtttgccgcc cgttgcccgg acgtgatgcc gatctgccgg
     1861 gaacagattt cgccgccggt cattatttcg ccgagccact gggtacggtg ccacatgtac
     1921 gatggccaga cgatggacac ggctgcgttg tagtcgaata cgttgcattt tttcgacatg
     1981 gcggtacgct aggcgcgatc tgaacgggga tggaggcggc aggtcggcgt ctctccgttt
     2041 gccgaagaaa ggcacaggaa ccgtgatcaa ggtaggtatc atcggctgtg gcgatgtggc
     2101 cacgcactcc tatatgccag gactgaacag gtttaatgac cgggcgaagg ttgtggccct
     2161 tttcgatatc gtcgaagagc ggatacagaa tgccaaagcg ttccatccca atgcaactgg
     2221 ttacaccgat tacggcgagt tcctcagcca cagcgatgag cggatggacc tcgttctgaa
     2281 tctcacgccg gcaccgctcc accgcgagat tacctctcgt gccctggagg ccggatacaa
     2341 cgtatattcc gagaagccgg tggcctcgac gctggaagat gcccaggaac tcgtgcagat
     2401 tgccgacagc aggggactta cctttttctg tgctccagca atgatggtga catcgcgcat
     2461 gatgtggctg aagcaacaga tggaggaggg cacgtttggc cgggcgtatt tcctgaaatg
     2521 tcaaatcggc agcatggggc cagcggcgtg gcgcgaatac tccggcgacc cacgtgtctt
     2581 ctataccccg aaagtcggtc cactcatcga tcttggcgtt tatatgttgc ataccatgac
     2641 ggggttcttt ggtcctgcca agcggatcca ggcagttggc ggcatcatga acgaggagcg
     2701 ggtcatgctt cagccggccc ggttcggcga gaagctcaag gtcgaagtcc cggacctgta
     2761 ttcgatcaac atcgagttcg agaacaaccg ctacggacat ctcttcaaca gcttcgccgt
     2821 gccgcgatcg aaagcaccga tgttcgagct ctacggctcg atgggtaccg caagcgtcgg
     2881 tctacctgag tggtatgacg caaacggcac taccgatctc tacctgcgag aagaatcagc
     2941 gagtggcgat aatgaattct ggcgggacgg tgttccggtg ccgaacccct caagggcggt
     3001 tggcgtcctc gactccgggc tgcttcatgc gttcgacgtg ctggagtacg gtgtgccgaa
     3061 catcctgacg gccgcgcatg cgacccatgt actggagatc atgctggcgt gtggcacggc
     3121 gcttgagacc ggcgagtcga tcgagatcac gtcgacattc gaggaagaag cacgcgccgt
     3181 caccacggca tgaaggagag tactctgtga aacgtgagca aatcggcgtc cagatgtata
     3241 ccctgaggga aatggcggca aaaaatctgg atgcgacact cggcgagatc gccaggatag
     3301 ggtatgccgg cgtggagatt gccgggttct atggtcattc cgcttcagat gtgaaatcca
     3361 tgctcgatcg gtatggtctg aaggcgttct ccgcgcacat cccggccgcc cagttcgagg
     3421 gtgacatcga aagcgtcgta agcgatctca cgacgatcgg tgccccgtgg ggaatcgtgc
     3481 cgtgggtgag ccccgaggag cgcaacgaga ccgcgatgcg cgatctcgga ttgaagatga
     3541 acgcttatgc cagccacctc aaggatgccg acatcaagtt cgggtatcac aaccacgact
     3601 tcgagttcac cgaaaaggtg ggtaccgggg aaaccctctt ccacacattg ctcaatatca
     3661 cagatcc
//
LOCUS       DASYWM010000014         1609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_94384, whole genome shotgun sequence.
ACCESSION   DASYWM010000014 DASYWM010000000
VERSION     DASYWM010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94384"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="VGR29_00580"
     CDS             complement(<1..1027)
                     /locus_tag="VGR29_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV2072111.1"
                     /translation="MATAPPPAVASSPEEFLRYQPNELGVVPILEYHVITTNPEEEEQ
                     FVRTADDMRADLQWLYDHNFYIVPLRDVVSSSIAVPAGKHPVALTFDDGSSTQFAFIE
                     NEQGELVPDPNSAIGILEEFSNAHPDFGRGGHFALLINNQLAWPDESQMPYFDAKIQW
                     LVEHGYEIGNHTMHHTNLTDIPNEEFKMTLAEPMIWADEVIGDRPENASRILTLPYGS
                     TPDEELHPDQREAMRRGFTYQGEHYEIIAALLVGADPAPSPASTVWNPLMIPRIQTFD
                     ESLAFWFGQFEAGEQVLYTSDGDPETIVVPNPLSVVLEGHLDVAALVAAGKQVIQYDL
                     ETGQLIDP"
BASE COUNT          314 a          483 c          506 g          306 t
ORIGIN      
        1 gtggatcaat gagctgccca gtctccagat cgtattggat cacctgcttc cccgcagcca
       61 ccagcgccgc gacatccaga tgcccctcga gcaccaccga cagcgggttc ggaacgacga
      121 tcgtctccgg atccccgtcg ctggtgtaga gcacctgctc gccggcctcg aactgcccga
      181 accagaaggc gagggactca tcaaaggtct ggatccgggg aatcatcagc ggattccata
      241 ccgtgctcgc cggagaggga gccgggtcag cacccaccag cagcgcggcg atgatctcat
      301 aatgctcgcc ctgataggtg aacccccgcc gcatcgcctc acgttgatcg ggatgcagct
      361 cctcgtcggg cgtcgaacca tacggtagtg tcaggattcg cgaggcattc tccggcctgt
      421 cgccgatcac ctcatctgcc cagatcatcg gctccgccag cgtcatcttg aactcctcgt
      481 tagggatatc cgtcaggttg gtgtggtgca tcgtatggtt tccgatctcg tagccatgct
      541 cgaccagcca ctggatcttg gcgtcgaaat agggcatctg ggattcgtcc ggccacgcca
      601 gctggttatt gatcaggagc gcgaagtggc ctccccggcc aaagtcggga tgggcattgg
      661 agaactcttc aagaatcccg attgcggagt tcggatcggg gaccagctcg ccctgctcgt
      721 tctcgatgaa ggcaaactgg gttgatgagc catcatcgaa ggtcagggcg accgggtgct
      781 tcccggccgg gacggcgatg gagctactga cgacatcccg caacggcacg atatagaagt
      841 tgtggtcgta gagccactgc aggtcggccc gcatgtcgtc cgcggtgcgc acgaactgct
      901 cctcttcctc ggggttcgtg gtgatgacgt ggtattcgag gatcgggacg acaccgagct
      961 cattcggctg atagcgcagg aactcctcag gtgagctggc gactgcaggc ggtggggcgg
     1021 tggccatggc cgtcggttcc gcggtcggtg cgaccgtcgg agtgcccgcc gggacggggg
     1081 tgccagggcc ggatggaggc tcagacaacc ctgatccagc tgactgttgg gagatcggtc
     1141 tctcgtcagc tacgggctgt actacatcgc ctgtgcccgc atctgcaaat aaggcaatga
     1201 tgatcgagac gagaagaacc agcacactgg cgtggcgaag cggcagagag agccggaccg
     1261 gcgtcggcac agggcgatca ctcctcaccg cgcgtggcgg cgatcaggac atctgacgta
     1321 ttggattaat gggatcccca gcagagctca cgctccccgg caatcctacc cccagttctc
     1381 gctggagcgc aacacgagcc gaagcgtttt tctcaagtca aagccggacg aaggatatca
     1441 ggcatcggac gcagccgcag cctggacggc aggtgaggca agcaattgga gactacgtgt
     1501 acgccctgaa ttgacggcga gtgattggtt cctgcgactt ggtgatggcc acgggcatct
     1561 ccacatccag cgtctggccc gtgaccggat catattggat cacctgctt
//
LOCUS       DASYWM010000015         5271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_356989, whole genome shotgun sequence.
ACCESSION   DASYWM010000015 DASYWM010000000
VERSION     DASYWM010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356989"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VGR29_00585"
     CDS             complement(<1..633)
                     /locus_tag="VGR29_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2072112.1"
                     /translation="MSTSNNIFFGINIDPSPDYGKALHSTQLAEHLGFDHALIQDHAY
                     NPGFVETWTLISALTGATSRIMLGPNVMTTPLRLPAMIAKEAATLNMISGGRLLLGLG
                     AGASDEGIVGLGGPDLREKGAKFRAFFDALHIVRGLWESEGEPFSHEGQELSVRDVHF
                     GPRPVRRIPIITGAMGPQSLRLTTKLADGISLSTTYVPAERLSWFRDQLDQ"
     gene            complement(948..1196)
                     /locus_tag="VGR29_00590"
     CDS             complement(948..1196)
                     /locus_tag="VGR29_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV2072113.1"
                     /translation="MYTGVTNDLQQRVWEHKNGVNTDGFTSGYTLTRLVYFAEFPWFD
                     DAIAYEKQIKGRLRWWKMGLVEERNPRWNDLAWDWFQE"
     gene            complement(1485..2162)
                     /locus_tag="VGR29_00595"
     CDS             complement(1485..2162)
                     /locus_tag="VGR29_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV2072114.1"
                     /translation="MIQPNQMTDDPHAHAQVLHYGAPLETATAVVVLIHGRGATAQSI
                     LQLAPGLVEGLDIARNVAFLAPQANGNTWYPHRFIAPIQDNEPWLSSALGLIDQVVDE
                     VASAGLEPKALMVAGFSQGACLSLEYAARGSRPIGAVAGFSGALIGPSEGPHHPMVDV
                     QDLPVFIGSGTVDDHVSPDLVRESARRFEEAGARVDVRIYEGMTHTINDDEIAAVRQT
                     IQLLPKT"
     gene            complement(2159..3202)
                     /locus_tag="VGR29_00600"
     CDS             complement(2159..3202)
                     /locus_tag="VGR29_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEV2072115.1"
                     /translation="MSTQTNLELTGIHHVSATTADAKANLDFYTRLLGMRLVKKTVNQ
                     DDISVYHLFYADGEGHAGTDLTFFNFPNLTNNIPGTGEVSETILRVTGKDAIEYWQKR
                     FDQFGIDHDDLTELAGHTLLRFRDFEGQRLALMDDGGDAAPIPGGNPWELATVPVEYG
                     IKGLGAISITTGRPAQTLAMLTDIMGFRVVDDQQGPEHPDHRIVLLETGKGGPNGMMV
                     VHVRPDLPLARTGRGGVHHVAFRVPTYEAHEEWNEYLRGKGVQVTEQIDRFYFKAMYF
                     REPGGVLYEISSDGPGFATDEQLDSLGEKLALPPFLEPRREQIEAYVKPLDSSKAVTT
                     TEYGQPVKEEANA"
     gene            complement(3223..3693)
                     /locus_tag="VGR29_00605"
     CDS             complement(3223..3693)
                     /locus_tag="VGR29_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEV2072116.1"
                     /translation="MVTDTTQLQSERLQDSRNLAVLTWVRLMRVYHKVDRRTAEEMKH
                     WKLSVSRFDVLNHAGVREGRTQQELADALLVTKGNICQLLDGMEADGLLRRRRSGRTN
                     HIYLTDEGRALREASLARHEERIAQEMSVLSSDEQQTLLTLLRKLDRSLSNDPA"
     gene            complement(3913..4845)
                     /locus_tag="VGR29_00610"
     CDS             complement(3913..4845)
                     /locus_tag="VGR29_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072117.1"
                     /translation="MPSSDQPIIPPSTGIPTPPGGAGAADLATDSTDPQILRELLDRA
                     RDRLAFYESFDRIIGENIRRSGELMVETVALREQAQALAAQSAMERAAFAATRQSERD
                     RYRALVEQVLNEVSTMRPLIDTMVTRFESVLEELSEDTDTETGAGGVLDSTSISIASA
                     LEAPSPESSETDEPPPVLLEDETPVTHINPPAEESAQETTEITTVEDAPPADVAPEVT
                     PEAPALEGSRGIDLLAHGVPNARVAISLQKMLRELDVVSSVEAREFANGELRLAIQAS
                     DALPEAALVAWLGDNNGELTSRSSSVTELTFNSA"
BASE COUNT         1000 a         1541 c         1690 g         1040 t
ORIGIN      
        1 ctggtcgagc tggtcgcgaa accacgaaag acgctcggcc ggcacgtagg tggtgctgag
       61 gctgatgccg tccgccagct tcgtggtgag gcgcagggac tgcggtccca tcgcgccggt
      121 gatgatcggt atccggcgca cgggacgcgg gccgaagtgg acgtctcgca ccgagagttc
      181 ctggccctcg tggctgaatg gctctccctc tgactcccag agcccacgca caatgtgcag
      241 tgcatcgaag aacgcccgga acttcgcgcc tttctcacgg agatcgggac cgcccaggcc
      301 aacgatgccc tcatccgatg ctccggcgcc gaggccgagc aggagccggc ccccgctgat
      361 catgttcaag gttgcagcct ctttcgcaat catggcaggc agccgcagtg gggtggtcat
      421 cacgttcgga cccagcatta tgcggctggt tgccccggtg agcgcggaga tgagcgtcca
      481 cgtttcaaca aagccgggat tgtaagcgtg gtcctggatg agtgcatgat cgaatccgag
      541 gtgctcggcc aactgcgttg agtggagggc cttgccgtaa tcgggagagg gatcgatgtt
      601 gataccgaag aagatgttgt ttgaggtcga catgcggtgg tccttttgga ggaagcgagg
      661 gtcgatgatg gaattcgaat gcggagcggt tcatcgcggc gcacagggcc aaacgatgga
      721 aggtgggaga agcaagcttc cactggtccg ggtcgaagca tgcaaccggt ctagccagcc
      781 ataggacagg accgctcggg caggatttac attgggcggt gccaacggag ccgagcggga
      841 gcggccccat ggctgagccg acagggccaa acaggaccgc tcaaacccat cgtcatcctg
      901 aggccgcagc cgaaggttcc tacgcgaagc gcatcaggag ggcgcaacta ttcctggaac
      961 cagtcccatg caaggtcgtt ccagcgcggg ttgcgctcct cgacgagacc catcttccac
     1021 caccgcaacc tccccttgat ctgcttctca tacgcgatcg catcgtcgaa ccagggaaac
     1081 tccgcgaagt acaccagccg cgtgagtgtg tatccggacg tgaagccatc ggtgttgacg
     1141 ccgttcttgt gttcccacac cctttgctgc agatcgttgg tgacaccggt gtacatggtg
     1201 cgggtcctgc ctagccatga tgtcgacgta ggccgttctc actaaacttc cgtgaaagcc
     1261 tcatccatgc tagtggcact ccaccacata ggctgtccag cctcatgaca ccatggtgat
     1321 gaacaccgct tcgcgtagga ttcatcaccc ttcgggatct gaatgacgat tggaggttga
     1381 gaggggggcg gcagggcagg tgtccaccgt acaggcaggc tccatatcgg ccctctggta
     1441 tgaaggacga agtcgttgct tgctcccggg aagtgatgca gtcgttatgt ctttggcagg
     1501 agctggatcg tctggcgaac ggcagcgatc tcgtcgtcat tgatcgtgtg cgtcatgccc
     1561 tcgtagatgc ggacgtcgac cctcgcgccg gcttcctcaa agcggcgggc gctctcccgt
     1621 acgaggtccg gtgagacgtg atcatcgacg gtgccactgc cgatgaagac cggcaggtcc
     1681 tgtacatcga ccattgggtg atgcggccct tccgacggcc cgatgagggc accgctgaat
     1741 ccagcgacgg caccaatcgg acgcgagccg cgagcggcgt attcgagtga gaggcacgct
     1801 ccctgggaga agccggcaac catcagtgcc ttgggctcca gcccagcact ggcgacctcg
     1861 tcgaccacct ggtcgatcag cccgagggcg ctcgaaagcc agggctcatt gtcttggatt
     1921 ggtgcaataa agcggtgggg gtaccatgta ttgccattcg cctgaggtgc aaggaaggcg
     1981 acattccggg caatatccag cccctccacg agacccgggg caagctggag gatgctctgt
     2041 gcggtcgcgc cccgaccgtg gatgagcaca acgacggcgg tcgcggtctc cagtggtgct
     2101 ccgtagtgga gcacctgtgc gtgggcatgt ggatcgtcgg tcatctggtt cggctggatc
     2161 atgcgttggc ttcttccttc accggctggc cgtattcggt cgttgtgacg gccttcgagg
     2221 agtcaagcgg cttcacgtag gcctcgattt gctcccgtcg tggctccagg aatggtggca
     2281 gggcaagttt ttcgccgagc gaatccaact gctcgtcggt tgcaaagccg ggcccgtcgc
     2341 tggagatttc ataaaggacg ccacctggct cgcggaagta catcgccttg aagtagaagc
     2401 ggtcgatctg ctccgtcacc tgaacgccct tgccgcgcag gtactcgttc cactcctcgt
     2461 gcgcctcgta ggtcgggacg cggaaggcga catgatgcac gcccccccgg ccggtgcggg
     2521 caagcgggag atctggccgg acgtggacga ccatcatgcc attcgggcca cccttgccag
     2581 tctccaggag gactatccgg tgatccggat gctccggccc ctgctgatcg tcgacgacgc
     2641 ggaagcccat gatatcggtc agcatggcga gtgtctgggc cgggcgacca gtcgtgattg
     2701 agattgcgcc gagacccttg atcccgtact cgaccggaac cgtggcgagc tcccacgggt
     2761 tgccgcccgg gatcggggcc gcatcgccgc catcatccat cagcgcaagg cgctgcccct
     2821 cgaaatcgcg gaagcggagc agggtgtggc ccgctagctc ggtgaggtca tcgtgatcga
     2881 tgccgaactg atcgaaccgc ttctgccagt actcgatggc atcctttccg gttacgcgaa
     2941 ggatggtctc gctgacctca ccggtgccgg ggatgttgtt cgtgagattg gggaaattga
     3001 agaaggtcag gtcggtacct gcgtggccct caccatcggc atagaagagg tgatagacgg
     3061 agatgtcgtc ctgattaacg gtcttcttca ccaggcgcat accaagtagc cgcgtgtaga
     3121 aatcgagatt ggccttggcg tcggcggtcg tcgcgctgac atgatgaatt ccggtcagtt
     3181 cgagattcgt ctgggtactc attgaatgat gtccttccat tactaggccg ggtcgttgga
     3241 gagtgagcgg tcgagctttc gaagcagggt caggagcgtt tgctgttcat ctgacgagag
     3301 gactgacatc tcctgcgcga tacgctcttc atgtcgcgcc agtgacgcct ccctgagagc
     3361 acggccctcg tcggtgaggt agatgtggtt ggtgcggccg gagcggcggc ggcgaagtag
     3421 gccatcggcc tccatgccgt ccagaagctg gcaaatgttg cccttggtta ccaggagcgc
     3481 atcagcgagc tcctgctggg tgcggccctc acggacacct gcgtggttga ggacatcgaa
     3541 acggctcacg gagagcttcc aatgcttcat ctcctcggcg gtacgccgat ccaccttgtg
     3601 gtagacgcgc ataagccgta cccacgtcaa tacggcgagg ttgcggctgt cctgcaggcg
     3661 ctcggattgt agttgggttg tgtcagtaac catcgttctc gtccttacgt gaagaaagtt
     3721 tagtattaaa ccttcgttgt gtcaagcggg aggaatggac cgtcgaggcc ccaagctgct
     3781 cttgacaggc gtgacgagct cgccaaggtg gaaccggagg aacggctcct tgctccttca
     3841 acggtcaagc gcgactatca accacgatcg tgaccggcac agtgacgtgg acgatggagg
     3901 ttatgtctta gatcaggcgc tgttgaaggt aagctcagtc actgaggatg atctgctggt
     3961 caactcaccg ttattgtcgc cgagccaggc aacgagggcg gcttccggga gggcatcgct
     4021 agcctgtatc gccaggcgaa gctccccgtt ggcaaactcc cgtgcctcca cgctgctgac
     4081 gacatccaac tcacggagca tcttctgcag ggagatagcg accctggcat tcgggacgcc
     4141 atgggcgagc aggtcaatcc ctcgggagcc ctccagggcc ggcgcctcgg gtgttacctc
     4201 cggggccaca tcggcgggag gagcgtcctc gacggtggtg atctccgttg tttcctgggc
     4261 ggactcctcg gctggggggt tgatgtgggt gactggcgtc tcgtcctcaa ggaggacggg
     4321 tggcggctca tctgtctcgc tggattcggg agacggtgcc tcaagggcgg aagcgattga
     4381 gatgctggtt gagtcgagga caccacccgc gccagtttcc gtatcagtgt cctcgctcag
     4441 ctcctccagg acgctctcaa accgcgtcac catcgtgtcg atgaggggtc gcatcgtgga
     4501 gacctcgttg aggacctgtt caacgagcgc acggtagcga tcacgttcac tctggcgcgt
     4561 ggccgcaaag gcggcgcgtt ccattgccga ctgcgccgca agtgcctgtg cctgttcacg
     4621 gagggcaact gtctccacca tgagttcacc agaccgacgg atgttttccc cgatgatccg
     4681 atcgaacgac tcgtaaaacg ccagccgatc cctcgcgcga tcgaggagct ctcgcagtat
     4741 ttggggatcg gtcgaatcgg tcgccagatc ggcggcgcca gctccgccag gcggcgtggg
     4801 gattcccgtc gatggcggga ttatgggctg atcggacgaa ggcatctggg ctccgtacat
     4861 atgacgcggc gtctaatctc tacagagggc agtgtagcaa accaactgcc ggtgccttct
     4921 gaggtggaca ccggggtata caattcaacg cagagaacgc ggactgtcgt gcacgggatt
     4981 gcacccagat cgtgagcccg cccgcaatga gggcagaccg ggccagagcg tccttacccc
     5041 cgtaccagac gtccattagg ccgcgtgagc ggccagcgtt gtcaaacaat ctccccaggc
     5101 cggaacctcc tggtgcgtgt gcacagggtg cacgtgtttt tcactctgga aagtcgagca
     5161 agagcaacca tgccgtcgcg aaaatccgct gacgatcagg cccaggccaa cgaaccaaac
     5221 gagcctatgg atcacgaaga ccagctgacg gctgaagcac cggcgccgaa g
//
LOCUS       DASYWM010000016        10315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_188155, whole genome shotgun sequence.
ACCESSION   DASYWM010000016 DASYWM010000000
VERSION     DASYWM010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188155"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1298..2506)
                     /locus_tag="VGR29_00615"
     CDS             complement(1298..2506)
                     /locus_tag="VGR29_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV2072118.1"
                     /translation="MHLTLLTLEEFRTYQQLALELPPEGLRIAGRNASGKTSLLEAIV
                     LLATTRSPRTSTERDTVRWESGEEYGVNPYARLGAVVVAKDRRHRLEISLELPEGTAR
                     IARKRFRVDGRSVRAHDMVGVLRTVLFSPEDVQLVTGAPAERRRQMDILISQIDRSYM
                     RALSRYGKVLSQRNGLLRTFSRDRVDPGSRAAVAQLSFWDEELVSSGSEIVSGRVVAL
                     ADLTSLVAERSRFLISDHEIGINYHPRLDLPSWSREEHGSLGELQQRVQAAFLSQLEQ
                     VRSEEFRRGTTVVGPHRDDFLMTLDGRPLDAFGSRGQQRLGVVALKLSESDMITNRTG
                     ERPIVLLDDVLSELDEVHRGLLLDALSAENCQVLVTSADRSPLEHPALEHLPMVQLSG
                     GKLIGNQAPA"
     gene            complement(2532..3674)
                     /gene="dnaN"
                     /locus_tag="VGR29_00620"
     CDS             complement(2532..3674)
                     /gene="dnaN"
                     /locus_tag="VGR29_00620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV2072119.1"
                     /translation="MRVACLQENLQRALSRVGRAVASRTALPVLSNVLIATDNGRLRI
                     AATNLDIGVTTWIDADIESEGRITVDARLLGEFVNTLPQGAISLTADDVRFSLTVQSG
                     RDKASINGIDAEDFPVIPSVPDEGFLVELDAQMLREMISLVEFSAATDESRPVLAGVY
                     TRFEGEMLTLASADGFRMAVSEHRLDNAAGDRQELIVPARSYRELARIIGDHADPIRI
                     AVTPNRSQVLARVGDTEWVSRLIDGTFPDVKQIVPKDFATRVDVGRDTLLNAVRRAGY
                     FARENNDVVFLMVQPGEDDLAPGNVEVSANAAERGNSQSFVDASVTGSEIRVAFNAKY
                     LTDVLSVLRHGQVMLGLNGSNHAGIVRPSGVEDYTHVIMPMVIGGQ"
     gene            3949..4560
                     /locus_tag="VGR29_00625"
     CDS             3949..4560
                     /locus_tag="VGR29_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072120.1"
                     /translation="MTQTAFDPSQHLTSIKGQEYLEVKWRLAWLRSEHPDAIVTTQLS
                     SHDGNRAIFSAQVELLGGGSATGWGSETADSFGNYIEKAETKAIGRALAALGFGTQFC
                     TDFDDGPETGVLADAPVQLRRPNQRGGIQESPAQYNNQMTAAQRAKILGISRELSITG
                     AQIEELSHSVTGSTFPELNKSQASRLIDSLMERKGEPTPRKAS"
     gene            4590..4814
                     /locus_tag="VGR29_00630"
     CDS             4590..4814
                     /locus_tag="VGR29_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072121.1"
                     /translation="MSPTRPQKRHNGVMGLDPDLEVVSVERLERRLEDGYRMIEDRLA
                     AGADVTELEAFWINLLLQYEALCDELPMAA"
     gene            complement(4900..5424)
                     /locus_tag="VGR29_00635"
     CDS             complement(4900..5424)
                     /locus_tag="VGR29_00635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV2072122.1"
                     /translation="MAKQWNKPFDNQLEEGKAYAATLETSHGTIEVEFFPEDAPIAVN
                     NFVSLAKEGFYDGTPFHRIVKGFVIQGGDPTGTGTGGPGYKFKDETIRKDYEQGMLAM
                     ANAGPNTNGSQFFIVLEDLRGRLPKNYTIFGKVTAGMDVVKSISDVQVTRSPSGEPSS
                     PVEPVMLNKVEIKS"
     gene            complement(5503..6111)
                     /locus_tag="VGR29_00640"
     CDS             complement(5503..6111)
                     /locus_tag="VGR29_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEV2072123.1"
                     /translation="MMEQENLELTGKTREDAWGLLTEWVESESLRRHCLSVETAMRAY
                     ARHYGQPEDAWGMLGLLHDFDYERHPARDEHTIVGADILASEGYPQWMSDALLAHGFD
                     ERFPRTTLMDKALFAVDELTGFIAAVALVRPSKAVEDVTYSSVNKKMKDRRFAEAVSR
                     EEMLAGAEELGVPFDQHVTFVIAAMAANAEALGLADVSLTNG"
     gene            complement(6117..7181)
                     /gene="queA"
                     /locus_tag="VGR29_00645"
     CDS             complement(6117..7181)
                     /gene="queA"
                     /locus_tag="VGR29_00645"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV2072124.1"
                     /translation="MTRAATPRLRMEEFDYELPPKLIAQEPLADRSGSRLMVLDRGTG
                     EVQHRRFVELPALLDAGDLLVVNDTRVIPARLLGRRETGARIEVLMLRPVADGGWLAL
                     ARPTRRLSPGERLIIPPRNGSREADASLVLREKLEDGQVIVDVDDTLSEHLEGYGRIP
                     LPPYISSELEDDNRYQTVYAQHRGSAAAPTAGLHFTDDTFAQLHERGVESAAVTLHVG
                     LDTFRPVTEEYAEDHQIHSEWCSVPPDTWEKILATRAHGGKVVAVGTTSARTLESLGQ
                     RVAAGHPGPFDGSTRIYITPGYEWTMVDGLVTNFHLPKSTLLLMISSFAGHESVLAAY
                     REAVRERYRFFSFGDAMLIM"
     gene            complement(7178..8284)
                     /gene="ruvB"
                     /locus_tag="VGR29_00650"
     CDS             complement(7178..8284)
                     /gene="ruvB"
                     /locus_tag="VGR29_00650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV2072125.1"
                     /translation="MNDERRPSGRQSTISNDRDAKRLLTPRQREDDDAGAERSLRPRR
                     LDEYIGQERVKKNLRIFIEAARARAESLDHVLLYGPPGLGKTTLASIIAAEMGVSLRV
                     TSGPAIERQGDLISILTNLQPGDVLFIDEIHRLNRVVEEVLYPAMEDFAVDIVIGKGP
                     AARTMRINLPRFTLIGATTRLALLTGPLRDRFGAVMRLEFYENDAMQQIIERSASILQ
                     LKLDPEGAKALSGRARGTPRIANRLLRRVRDVAQVVGEGIVTPDIANHALELLEIDHL
                     GLDDTDRRVLDAVITKFDGGPVGIDTLAAATSEETDTIMDVYEPYLIQLGFLQRTPRG
                     RIATRRAYEHLGYEISAKVPEFSGQQQQLFREDE"
     gene            complement(8321..9004)
                     /locus_tag="VGR29_00655"
     CDS             complement(8321..9004)
                     /locus_tag="VGR29_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2072126.1"
                     /translation="MQRPRRVRPQPVPRSSARPTPREAWDRPGNRIHIVYIVISSLVV
                     CSMLAVALATIDFSGLFGGDDENSANIVDPNADLISEQETKVAGNPNDVDEVVLLANL
                     LGNTGRIRDAVPWYEKALELAPDDHGIRLDFARSLAGADLLPDAEAQFLRVLEDDPDN
                     QSAHYYLAELYMRWDSARREDAATHYRRAVEIDPASFLAERAQIELDAMGSGAPFPSP
                     LPSTPPPGT"
     gene            9148..9220
                     /locus_tag="VGR29_00660"
     tRNA            9148..9220
                     /locus_tag="VGR29_00660"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9181..9183,aa:Thr,seq:cgt)
     gene            9244..9330
                     /locus_tag="VGR29_00665"
     tRNA            9244..9330
                     /locus_tag="VGR29_00665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9278..9280,aa:Leu,seq:cag)
     gene            9404..9750
                     /gene="rnpB"
                     /locus_tag="VGR29_00670"
     ncRNA           9404..9750
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGR29_00670"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            9762..9836
                     /locus_tag="VGR29_00675"
     tRNA            9762..9836
                     /locus_tag="VGR29_00675"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9796..9798,aa:His,seq:gtg)
     gene            complement(10101..>10315)
                     /locus_tag="VGR29_00680"
     CDS             complement(10101..>10315)
                     /locus_tag="VGR29_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072127.1"
                     /translation="ITVITSLILTFVGSEEQAPDRLIIIVLGLTALVALSRSAWFNRM
                     LIPIIQRALSMPQQILPRFERFLVSD"
BASE COUNT         2101 a         3201 c         2974 g         2039 t
ORIGIN      
        1 cgcagatcca cacgtgggcg gcaactctat cgtggctgcg cgggaggtgc tttcttctct
       61 catcatgcgt ggactcgacg gaaataccat gccgttcgtt tcatggaatg aggacgatga
      121 cctctggtac atgccgcgca cgttgcatgc ggctatctac ctccatttgt ggcgagacct
      181 ctctaaccag aagcggctcc aggttccgtg ccagcattgc aatcagttct acacccccac
      241 ccggagcagt cagaaatact gcagcaccca atgcaaacag aatgcgtata ccgctcgcaa
      301 aagagcgcga gcctgaacgg ctactccacc ccagaattac cccaaaaccg agggacagca
      361 acggacgatt gcagacacag tcaagccata atccttggat taccagtcat agaggacagg
      421 gtgatacgta tacggacgga tcgctgtaat actctgaatc aggcagttgg ggttcgaatc
      481 cctgcacccc agccaacgac aagcgtcccg gtccttggct ggggcgtttt tgcgtctata
      541 ggcgcacttc ataaaacaga gaagacgaag cctatttcct tcgccgtcac cgatcgttgc
      601 tgtttcctgg ctgacatgcg ggtggtcgta cgagtaatcc ttgacgtgcg cttgtgatga
      661 tccccgggca taacgcggag gaatactccc ttgagggcga gaagcgcgtt agcgccaacc
      721 tcaagtgcgt ggacctgccg tgacacgctg cgctgggaca tcagtccatt ggcacgaccg
      781 agcgtaaccg cgaccattct caaggtaacg cccaacgcgt ggatgtgcct tgacccctgt
      841 ggccatcgat caagtacaca tcggccttct agcgcgcttc cacaggtaga cgccctccgt
      901 ggggaggact cagcgcatcc ctcgctgatg taccctcgct ccctttggca caacaagaag
      961 gcggccaaac gtcgcgcgcg ctcactaaac gtgttgcgtt ggttttagca cccgaccgga
     1021 acgaaagatc cgttgtgctg agcagtccga aatctagtcg cgctatgaac cgtggatgtc
     1081 agcaatattg gtgaaagtct tgatcccggc tataccacca cgctctcgct gcatgaccaa
     1141 gagcgctcat tgggttgcgc cgtaactggt gatgaagcga cgaacgccgg ttccacattt
     1201 caccatatca ggtgggtagc agctgctgga ggaacctgtc ctccgcaaga ctcaccgcac
     1261 tcatttggtc gtcgggaccg cctgcctgat gcacggctta cgccggtgcc tggttgccga
     1321 tcaactttcc gcccgagagc tggaccattg gaaggtgctc gagggctggg tgctccagcg
     1381 gcgaccgatc cgcggacgta accagcacct gacagttctc cgccgataac gcgtcaagca
     1441 gcaacccacg atgcacctca tccagctcag acagcacatc atcaaggagc actatcggcc
     1501 gttcgccggt cctgttggta atcatgtcac tttcggacaa cttcagggcc accaccccga
     1561 gccgttgctg tcccctggag ccgaaggcgt cgagaggccg accgtccagc gtcatgagga
     1621 aatcgtcccg atggggccca accactgtcg tccccctgcg gaactcttcc gaccgcacct
     1681 gctcaagctg agacagaaat gcagcctgca cccgctgctg caattcgccc aggctgccgt
     1741 gctcttccct gctccacgag ggcaggtcga gccgaggatg gtaattgatc cctatctcgt
     1801 ggtctgagat gaggaatcgc gatctctccg ccaccagact agtgaggtcg gctagtgcga
     1861 caaccctgcc ggaaacaatc tccgaccccg agctcaccag ctcctcatcc cagaacgaga
     1921 gctgggcgac cgctgcacgt gaaccggggt cgacccggtc gcgtgagaac gtacgaagca
     1981 agccgttgcg ctgcgaaagc actttcccgt accgcgacag ggcccgcatg taggagcgat
     2041 cgatctgcga gatgagaatg tccatctgtc gccgacgctc ggccggcgcg ccggtgacca
     2101 actgcacatc ctcaggtgaa aacagcactg tacggagaac gccgaccatg tcatgagcac
     2161 ggacactccg gccatctacc cgaaatcgct tccgggcaat gcgcgccgta ccttccggta
     2221 gctccagcga gatctccagg cggtgtctgc ggtctttcgc gaccactacc gcgccgaggc
     2281 gagcatacgg gttcacccca tactcctccc cgctctccca gcgcacggta tcccgctcgg
     2341 tggacgtccg cggtgatcgt gtggtagcaa gcaagacgat cgcttcgagc aggctcgtct
     2401 ttccgctggc attccgtccc gcgatgcgca atccctcggg tggcaattcc agtgccagtt
     2461 gctggtaagt tcggaattcc tcgagtgtga gcagtgtcag gtgcatagga tccggagtcc
     2521 cggacaatgt gctactgacc gccgatgacc atcggcatga tcacgtgcgt gtaatcctca
     2581 acgccggacg gacggacaat tcctgcgtgg ttcgagccgt tcagacccag catcacttga
     2641 ccatgtcgaa gcacggacag aacgtccgtc aggtacttgg cattaaaggc aacacgaatc
     2701 tccgaccccg tgacacttgc atcgacaaag ctctggctgt tgccgcgctc ggcggcgttg
     2761 gccgacacct caacgttgcc cggagcaagg tcgtcctcac ccggctggac catcaggaag
     2821 accacatcgt tgttctcgcg ggcgaagtag cctgcacgcc tgacggcatt gagcaacgtg
     2881 tcgcgaccga catcgactcg cgtggcgaaa tccttgggca cgatctgctt cacgtcggga
     2941 aacgtgccgt caatgagccg cgacacccac tcggtgtcgc ccacccgcgc aagcacctgg
     3001 ctgcggttag gagtaacagc gattctgatc gggtcggcat ggtcgccgat gatccgcgcc
     3061 aattcccggt aggagcgggc tgggacaatc aactcctgcc ggtcacctgc ggcattgtcg
     3121 agcctgtgct ccgataccgc catgcgaaag ccatcggcag atgcaagggt cagcatctcg
     3181 ccttcaaacc gagtgtagac tcccgcgagc accggccggc tctcatcagt ggccgcagaa
     3241 aattcgacga gcgagatcat ctcccgcaac atctgggcgt caagttcgac caggaacccc
     3301 tcgtcgggaa cagaagggat gacaggaaaa tcctccgcat cgatgccgtt gatactcgcc
     3361 ttgtcgcggc cagactgcac ggtaagcgag aagcgaacgt cgtcggccgt gagtgaaatc
     3421 gctccctgtg ggagcgtgtt cacgaactcg ccaagcagtc gggcatcgac cgtgattcgc
     3481 ccttccgact caatgtcagc atcgatccac gtcgtgacac cgatatcaag attggtcgca
     3541 gcaatgcgta gtcgcccgtt atccgttgcg atcaacacat tactgagaac cgggagcgca
     3601 gtacgggacg ccacggcccg tcctacccga ctcagcgcgc gctgcagatt ttcctgaagg
     3661 catgcgaccc tcacgggctt acctctcttc tgtgagactg atcgtagacg gccacgagta
     3721 tagcaaagcg tctgctaatc cctgtcggct ccatccaaac atcctctagc aacaccatgc
     3781 tcgtctgctg gcatggcgac gattccgcct gcgtattctc tcacgaatgc gggccccatg
     3841 gcgtccggtc caccaccgtt gacgccttga caaagtagat catccgttct attattcact
     3901 tagaacagag gttcttattc gaccatcgcg gaagaacgag gaagacacat gacacagaca
     3961 gcgttcgatc cgtcccagca tctcaccagc atcaagggac aggaatatct ggaggtgaag
     4021 tggcgtctgg catggctccg atcggaacat ccggatgcaa tcgtcaccac acagctctca
     4081 agccatgatg gtaaccgcgc catcttttcg gcccaggtcg agcttctcgg cggagggagt
     4141 gccaccggtt ggggaagcga gactgctgat agtttcggga attacatcga gaaggcggaa
     4201 acgaaggcga tcggccgtgc gctcgcggcg ctcggattcg gcacacagtt ctgtaccgat
     4261 tttgatgacg gtccggagac tggtgtcctg gcggacgctc ccgtccagct tcgccggccg
     4321 aaccagcgcg gtgggatcca ggagtctcct gcgcagtaca acaaccagat gacagccgcc
     4381 cagcgcgcga agattctggg gatatcgcgg gaactgagca tcacgggtgc gcagatcgag
     4441 gaactttccc actccgtcac aggctccacg tttccagagt tgaacaagag ccaggcgtcc
     4501 aggctgatcg acagcttgat ggagcgcaag ggcgagccca ctccccgcaa ggcatcctga
     4561 tcctcgcggg gtaacggaag gagcacaaca tgagccccac ccgaccgcag aagcggcata
     4621 atggtgtcat gggactcgat ccagaccttg aggtagtttc ggtcgaacgg cttgaacgac
     4681 gcctcgaaga cgggtaccgg atgatcgagg accggctcgc agcaggcgcc gatgtgacgg
     4741 aacttgaagc gttctggatc aatctgttgc tccagtacga agcgctctgc gatgagctgc
     4801 caatggcagc ctagcagtgt ggcgggacaa gtagaacggc gggcaaagga gatagtcctt
     4861 gcccgccgtt ctacttgtcc gtgacgtcac ggccgttgcc tagctcttga tctcgacctt
     4921 gttgagcatg actggctcca ctggtgagga gggttccccg gacggcgaac gtgttacctg
     4981 cacatcggaa attgacttca caacgtccat acctgcggtc accttgccga agattgtgta
     5041 gttcttcggg agccggccgc gcaggtcttc aagcacgatg aagaactggc ttccgttggt
     5101 gttcgggccc gcgtttgcca tggcgagcat tccctgctcg tagtccttcc gaattgtctc
     5161 gtccttaaac ttgtaccccg gtccgccggt gccggttccg gtcgggtcgc cgccctggat
     5221 gacaaacccc ttcacgattc gatggaatgg ggtgccgtcg tagaaccctt ccttcgccag
     5281 gctgacgaag ttgttaacag caatcggagc gtcctcgggg aagaactcga cttcaatggt
     5341 gccgtggctc gtttccagcg tggcagcata agccttgcct tcctccagtt gattgtcgaa
     5401 tggcttgttc cattgcttcg ccatgaatcg gatgtccttt cgttcggacc tttaggaggt
     5461 ccatttggtc caggtttctc tcaggtccgg agttgatagg cgctagccgt tggtcaagga
     5521 tacgtccgcc agacctaacg cctcggcgtt ggcggccatt gccgctatga caaaggttac
     5581 gtgctggtcg aaaggcacgc caagttcctc ggctcccgcc aacatttctt cgcggctcac
     5641 ggcctcggca aatcgccggt ccttcatctt cttgttgacc gaggaatagg tgacgtcctc
     5701 gacggctttc gacggtcgca ctagagcgac ggctgcgata aaaccggtca gctcatcgac
     5761 ggcgaagagc gctttatcca tgagcgtcgt gcggggaaac cgctcgtcga aaccatgcgc
     5821 gagcagcgcg tctgacatcc actgcggata accctcgctg gctaggatgt cggcgccgac
     5881 aatcgtgtgc tcgtcccgcg ccggatgtct ctcatagtca aaatcatgaa gaagccccaa
     5941 catgccccag gcgtcctctg gttggccgta gtgccgggca tacgctcgca tcgccgtctc
     6001 taccgagagg caatggcgcc ggaggctttc gctttccacc cattcggtga gcagccccca
     6061 tgcatcctcc cgtgtcttcc ccgtaagctc aagattctcc tgctccatca tttctcctac
     6121 atgatcagca tcgcgtcacc gaacgaaaag aaacgataac gctcccgaac tgcctcccgg
     6181 taggcagcaa gcacactttc atgaccagca aatgagctga tcatcagcaa gagtgtcgat
     6241 ttcggcaggt ggaaattcgt cacgagcccg tccaccatcg tccactcgta gcccggcgtg
     6301 atgtagatcc gcgtagatcc gtcaaatgga ccaggatgac ccgccgccac ccgctgcccg
     6361 agcgattcaa gcgtgcgcgc cgatgttgtg ccgactgcta ccaccttacc accgtgtgcc
     6421 ctcgtcgcca ggatcttttc ccacgtatca ggcgggaccg agcaccactc actgtgaatc
     6481 tggtggtcct cggcatactc ctcggtcacc gggcggaatg tgtcgagccc aacatgcaag
     6541 gtgactgctg cagattcgac gcctctctca tggagctgtg cgaacgtgtc gtctgtgaaa
     6601 tgtagaccgg cggttggtgc ggcggcagag ccacgatgct gggcgtacac cgtttggtag
     6661 cggttatcgt cctcaagttc gctggagata tagggtggca gcgggatacg tccatagccc
     6721 tccaagtgct ccgagagcgt atcgtccaca tcaacaatga cctggccgtc ctcgagtttc
     6781 tcgcgcaaca caagcgatgc gtcagcttct cttgaaccat tgcggggcgg aatgatcagg
     6841 cgctcaccgg gactcaggcg ccgcgtcggc ctggccaagg caagccatcc tccgtccgcc
     6901 acaggccgga gcatcaacac ctcgatcctt gctcctgttt cgcgacgccc gagcaagcgc
     6961 gccggtatga cgcgggtgtc attcaccaca agcagatcgc cggcatcgag caacgccgga
     7021 agctcgacga atcgccggtg ctgcacttcc ccagtcccgc ggtccagcac catcaggcga
     7081 cttccggatc gatccgccaa cggctcctgg gcgatgagct tcggggggag ctcgtagtca
     7141 aactcctcca tacggagccg tggagtcgct gctctggtca ctcgtcttcc cggaagagct
     7201 gctgctgctg acccgaaaac tccggtacct ttgcagagat ctcgtatccc agatgctcgt
     7261 atgcgcgccg ggtggcaata cgtccgcggg gggtgcgctg gagaaaccca agctggatca
     7321 ggtaaggctc atacacatcc atgatcgtgt cagtctcctc actcgtcgca gcggcgaggg
     7381 tatcgatgcc aactggtccg ccatcgaact tggtgatcac cgcatcgagc acccttcggt
     7441 ccgtgtcatc gagacccaga tggtcgattt ccagcagttc gagcgcgtga ttcgcgatat
     7501 ccggcgtcac aatcccctcg cccacaacct gggccacgtc ccggactcgc ctcagcaacc
     7561 ggttggcgat gcgcggcgtt ccacgagcac gaccggacag ggccttcgcc ccttccggat
     7621 ccagcttcag ctgcaggatc gaagctgagc gctcgatgat ctgctgcatc gcatcgttct
     7681 catagaattc gagccgcatc acggcaccaa agcggtcacg tagcggtccc gtcaacagcg
     7741 ccagacgggt cgtcgcccca atgagtgtga agcggggaag gttgatgcgc atagtccgcg
     7801 cagctggccc cttcccgatc acgatatcga ccgcgaagtc ctccattgcc gggtacagga
     7861 cctcttccac cacccgattc aaccggtgga tctcgtcgat gaagagcaca tcgcctggct
     7921 ggagattggt caggatcgag atgaggtcgc cctgacgctc gatcgcagga ccggacgtca
     7981 cccgcaggct gacacccatt tccgctgcga tgatggacgc aagcgtcgtc ttgcctaaac
     8041 ccggtgggcc gtacagcagg acgtgatcga gcgactccgc cctggcacgc gctgcctcga
     8101 tgaagattcg caggttcttc ttaacccgct cctgcccgat gtattcatcg agccggcgag
     8161 gccggagtga acgctctgca ccagcgtcgt catcctcccg ctgccggggc gtgaggaggc
     8221 gcttcgcatc acgatcattc gagatggtgg actgtcgccc gctcgggcgt cgctcgtcgt
     8281 tcatactccc attataccgc gagtatcgaa catacgttct ctatgtaccc ggaggaggcg
     8341 tggacggcag agggctcggg aagggcgctc cgctgcccat cgcgtcgagc tcgatctggg
     8401 cacgctcagc gagaaatgag gcggggtcga tttccactgc ccttcggtag tgcgtagccg
     8461 cgtcctcccg cctggcagaa tcccaccgca tgtagagctc cgccaggtag tagtgggcag
     8521 attggttgtc cggatcgtcc tccaacactc gaagaaactg cgcctcggcg tctggcagga
     8581 ggtccgcgcc ggcaaggctc ctcgcgaaat cgaggcggat cccgtgatcg tccggagcga
     8641 gctcgagcgc cttctcgtac catggcacgg cgtcacgtat tcggccggtg ttcccgagta
     8701 ggttggcgag aaggacaacc tcgtctacat cgttggggtt cccggccacc tttgtctcct
     8761 gctcggagat gagatccgcg tttgggtcca cgatatttgc gctgttctcg tcgtctcctc
     8821 cgaagaggcc ggagaaatcg atggtcgcca gggccacggc gagcatggag cacaccacga
     8881 gcgaggagat cacgatgtag acgatatgaa tcctgttgcc cggtcggtcc catgcctctc
     8941 tcggcgtcgg ccgagcggag cttcgcggaa ctggctgtgg tcgcaccctt cttggccgct
     9001 gcactctctg gtccttaagg tttgtaccgg ctacacttaa ccgcagcgat ggccgccgct
     9061 catgtaacgc aagagagtac accaggaacc gccgttggcg gacacaccga gccctctgct
     9121 atactgacgc ggcttcgcag caggagcgcc ggcctagctc agttggtaga gcagctgttt
     9181 cgtaaacagc aggtcgccgg ttcgagtccg gcggtcggct ccgcaagctg caccgcacgc
     9241 caggccgatg tggcggaaca ggcagacgcg ctacgttcag ggcgtagtgg gagtaatccc
     9301 gtgggagttc gaatctcccc ttcggcacca ctccggataa tcgacgatcg atgctgatcg
     9361 tgatgctccg ggtgaacgca ccttcagaag cgaatgtgaa gtggaagcaa agggcatggt
     9421 cgctgggcct ttcaagagga ccagaggaaa gtccgggctg cagagagcaa gggtgcctgg
     9481 tgaaagccag ggaggtgcga ctcgtcgcat cgacagtcca gatcggcgca ctcaataggg
     9541 tgcgagagca acagagacaa aggccgcgac tccggtcgcg gagtgaaaag ggcaatcctc
     9601 cccgcagcaa tctccgattc gaccgttagc aggctgctcg ccaaggtcgg aagtcgagag
     9661 ccgtcacgaa ggcgacgacg tggccaatcc tttcggattg agagagatga ccatccaaaa
     9721 cagaacccgg cttactcctt ttctcccctt tcacatctcg tggtgcttat agctcaatcg
     9781 gcagagcgct tgcctgtgga gcaagaggtt ccgggttcga gccccggtag gcacccccga
     9841 aaagcctata acggtaagga aagcggccac ccgctctgag aggccgttcg tcctatcctc
     9901 actgcgctgc acctgcgctg cacagccgtc gatatcgacg gttccgcccc agcctgtcga
     9961 gccgtccctg ggaaaccttg gcaggcccct ccgtatacat cccctactcc ccactggtga
    10021 cggctagagg aaaacagcct ccccatgtga ggggccgtac gcgtttctgg tcttctggtg
    10081 cattttgagg tcacggccag ttaatcactc accaagaatc gctcaaatct cggcagtatt
    10141 tgctgtggca tgctcagggc gcgttggatg atcgggatca gcatgcggtt gaaccacgcg
    10201 ctccgggaca gcgcgacgag ggcggtcaag ccgagaacga tgatgatgag gcgatccggg
    10261 gcctgctcct cggagccgac gaaggtgagg atcagtgagg tgatcaccgt gatgg
//
LOCUS       DASYWM010000017         4145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_169465, whole genome shotgun sequence.
ACCESSION   DASYWM010000017 DASYWM010000000
VERSION     DASYWM010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169465"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VGR29_00685"
     CDS             <1..268
                     /locus_tag="VGR29_00685"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEV2072128.1"
                     /translation="PRWTSWPWTDPSVIDNPFELQVENRLPDDVISSLEERGHAVRLQ
                     DAWGGGGAAQIIARNPDTGLLIGGTDARVEGSVLGFYASGSHSI"
     gene            complement(344..1324)
                     /locus_tag="VGR29_00690"
     CDS             complement(344..1324)
                     /locus_tag="VGR29_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV2072129.1"
                     /translation="MTTTTTFPQHTRSLAADAPVDLHLHTFVSDGIWHPRDLIDRLEI
                     DGISVAAVCDHDNQRSVVEAIEYGRRRNVLIIPGIECTVRWADRQWHLLVYGIRPDED
                     RLEAKAFLDLVARQDEIFQALAKDARCRVERSGRPIPTVNSLERHGPLMPVHVLRAMI
                     ADKHVSRLKEAAELLVKLGGNFTTDTPLAEVVAAAHEGGGLCIVAHPGRADLGPALTE
                     EMLDQMLAEVPVDGIECHYRTYTDKETAYYRGLAEMRGLLIGTGSDSHGPGVPVDPRP
                     FHASWSADLLARLGLEVDSSEGPVWEPGMDPNAVPEPEPVPAHEQVPAQG"
     gene            1748..2242
                     /locus_tag="VGR29_00695"
     CDS             1748..2242
                     /locus_tag="VGR29_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072130.1"
                     /translation="MLKVLIPIVAVLALLLGFAATTAQDASPVATDQHPFVGTWLFDA
                     NAEDPANAPDVVRVSADGGYISVNAAGFTSLGVWEPTGEQTAIVTVTSTGVDETGAPA
                     GTFVLRASAEVDASGDAFTASYTSEVVDPDGAGTGEAGPTTATATRLTPEPMGTPVAG
                     TPAG"
     gene            complement(2359..2652)
                     /locus_tag="VGR29_00700"
     CDS             complement(2359..2652)
                     /locus_tag="VGR29_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072131.1"
                     /translation="MSVVFFSDTPNVTRDLVERVSGFVNEHLGGSPPDGSLVHADGPT
                     DGGGWWSFELFESEAQFTRFNEGILTPAFVQAGMNPPAFRRLEVAWDSTQVPG"
     gene            complement(2861..4075)
                     /locus_tag="VGR29_00705"
     CDS             complement(2861..4075)
                     /locus_tag="VGR29_00705"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2072132.1"
                     /translation="MTEAKGQARNAFPSRDIRVDGVTLTGGSVDGVGSRSTDLEYVDV
                     VIVGGGQAGLATGYHLARRGIRFVIVDAHSSVGDSWRNRWDSLKLFTPARYAGLPGKP
                     FPAPPDSHPSAAEVATYLESYARQMGLTVQCGVRVDRVTPLVGDAKTGFLIYTSSGLL
                     KANQVVIATGGYQKPNIPFFAGELGPDILQLHSSEYRNPSQLREGPVLVVGASTSGVE
                     IALEAAQNHRTLLSGRHPGQIPFRIDGPLARLTFPLVWFIWNHVFAMANPLGRMTRPY
                     VRHRGGPLFRIKLADLEAANVERVTLRTIGAQAGQPVLEDGRVLDVTNVIWCTGFRGD
                     YSWIDGLSIGDDGYPVENLGTVPELPGLYFVGLKFQRTIGSMLIGGVGRDVAYIADCI
                     ATARQEHDSGDG"
BASE COUNT          834 a         1279 c         1254 g          778 t
ORIGIN      
        1 gccgcgctgg acgagctggc catggacgga cccgagcgtc atcgacaatc ccttcgagct
       61 tcaggttgag aatcgcctgc cagacgacgt catctcctcg ctcgaagagc gagggcacgc
      121 tgttcgtctc caggacgcct ggggcggtgg cggcgcggca cagatcattg cccgaaaccc
      181 ggataccggc ctactcatcg gtggtaccga cgcacgagtc gagggcagcg ttctcgggtt
      241 ttacgcatca ggatcacact ctatttgatg cgcaaccccg gcacggcgcg ggtagccggg
      301 agaatggagc ctccataggg aagaagtgaa gctcctgcca cgatcagccc tgtgctggaa
      361 cctgttcgtg ggctggcacg ggctccggtt ccggcaccgc attcggatcc atgccgggct
      421 cccacactgg cccctctgac gagtccacct caagccctag ccgcgcgagg agatcagccg
      481 accaggatgc atggaaggga cgaggatcta ccggcacgcc gggcccgtgg gaatccgatc
      541 ctgtcccgat cagcaggccc cgcatctcgg cgagaccccg ataataagcg gtctccttgt
      601 cggtgtaggt gcggtagtgg cactcgattc cgtcaaccgg cacctcggcc agcatctggt
      661 ccagcatctc ttccgtcagc gcgggaccga gatccgcacg cccgggatga gccacgatgc
      721 agagcccgcc accctcgtgt gcagccgcaa ccacctcagc gagaggcgtg tccgtcgtga
      781 aattgccgcc aagcttcacc agcaactcgg cggcctcctt caatcgcgac acgtgcttgt
      841 cggcaatcat cgcccggagc acgtgaaccg gcatcaacgg gccgtggcgt tccagggagt
      901 tgaccgtcgg gatggggcgc ccggaccttt ccactcgaca gcgggcatct ttcgccaaag
      961 cctgaaagat ctcgtcctgc cgagcgacga ggtcgaggaa ggccttcgct tccagtcgat
     1021 cttcatccgg ccggatgcca tacacaagca ggtgccattg acggtccgcc cagcgaaccg
     1081 tgcactcgat cccgggaatg atcagcacat tgcgccgccg cccgtattcg atcgcctcga
     1141 cgaccgagcg ctggttgtca tggtcgcaga cggccgccac gctgataccg tctatttcca
     1201 gtcgatcaat caggtcacgc ggatgccaga tcccgtcgct gacgaaagtg tgtaggtgaa
     1261 ggtcgacagg cgcgtccgcg gccaatgatc gagtatgttg aggaaacgtg gtcgtagtgg
     1321 tcacagggtg gaaatgctcc cgagcgagtt agccgaactg ccggcacctc caagcacggt
     1381 gccgaagcgt ttgcaattat agttcggcta gttgcccctt ccggtgtttt ttgctccctc
     1441 cccggatgtc agggcctccc actcatggac atccgtgacc gcccggttgc gattcccacc
     1501 atcacccggc atcggtgggc ctacttgagg ttgtgtcaaa atacaagggt cgcacgcaaa
     1561 cgcatcctcg acatgagctg attcctttag gcataccctt tgcgtatacc tcgacaatga
     1621 caggcttgat tcaggcgaca aacaggattc cgagatccgg gcggcccggt gccgtgaggg
     1681 ggtgaactgt accaagcagt gatttgagct gtccgagatt cacgttctga atgccaggag
     1741 aattaccatg ctcaaggtgc tcatcccgat cgtcgccgtc ctcgccctcc ttttggggtt
     1801 tgccgccacg acggctcagg acgcttcacc agttgccaca gatcagcatc cgttcgtcgg
     1861 aacctggttg ttcgatgcaa atgcagagga tcccgccaac gcgccggacg tggttcgcgt
     1921 atcagccgat ggaggataca tctccgtgaa cgcggcgggg ttcacgtcac tgggggtttg
     1981 ggagcctacc ggcgaacaga cggcaattgt gaccgtcacc tcgacaggtg tggacgaaac
     2041 aggtgccccg gcaggaacat tcgtgctccg cgcttcggca gaggtggatg cctcgggaga
     2101 tgccttcact gcttcgtata ccagtgaggt agttgatccg gacggcgctg gcacgggcga
     2161 agctgggccc acgacagcca cagctacccg tctcacccct gagccaatgg gaacgcccgt
     2221 ggcggggacg cctgcaggat aggcagggct catacagcct ggcccttcgt tgaatacatg
     2281 tgaggaacac gaagacgcgg ctctcgaagg tgtcgggagc cgcgtcttcg aattatatgt
     2341 atcctgcggc ggccgccgtt agccgggtac ttgcgtgctg tcccaggcga cctccagccg
     2401 gcggaacgcg ggcgggttca tacccgcctg tacgaaggca ggtgtcagga ttccctcgtt
     2461 aaaccgggtg aattgtgcct cggactcgaa gagttcaaag gaccaccacc cgccgccgtc
     2521 ggtcggcccg tcggcatgga cgagggaacc gtccggaggg gacccgccaa ggtgttcgtt
     2581 gacgaagccg ctgacccgct cgaccaagtc tcgcgtgacg tttggagtgt ctgaaaagaa
     2641 gacgaccgac atgctgacct cctccatccc caagggatgg gtgatgatga acactgacgg
     2701 tgaggagaca ggaactgctt ggatcgtacc agtcatcaga ccagaaagat acaggcgtga
     2761 ctcgcacgtt tttggtgggt acgacggtac ccgcagggag caggagcttt gtcgcacgat
     2821 caggtgcgcg actgcccgtg cctgagcgag caggctgccc ctagccatct ccagaatcat
     2881 gctcttgtcg tgcggtagca atgcaatcgg cgatgtaggc aacgtccctg ccgacgccac
     2941 cgatcaacat cgagcctatg gtccgctgga acttgagacc gacgaaatag agcccgggga
     3001 gttccggcac tgtcccaagg ttttcgactg gatagccatc gtccccgatt gacagcccat
     3061 cgatccagct gtagtcaccg cgaaatccgg tacaccagat cacgttggtg acatcaagca
     3121 ctcggccgtc ctccagcact ggctgccctg cctgggcgcc gatcgttcgt agcgtgacgc
     3181 gctcgacatt ggcggcctcg agatccgcca gcttgatcct gaaaagcggg ccaccacggt
     3241 ggcgaacata gggccgagtc atcctgccga gcggattcgc catcgcaaat acatggttcc
     3301 agatgaacca gacaagggga aacgtcagtc gcgcgagagg gccatcgatg cggaagggga
     3361 tctgcccggg atggcgtccg ctaagcagag tccggtggtt ctgcgcggct tccagggcga
     3421 tttcaacacc tgaagtgctt gcccccacga caaggacagg gccctccctc agctgggacg
     3481 ggtttcgata ctcgctcgaa tgcagctgca gaatgtcagg cccaagttcc ccggcgaaaa
     3541 atggaatgtt cggcttctga tatccgccgg tcgcaatgac cacctgattg gccttgagta
     3601 acccgctact ggtgtaaata agaaagccgg ttttagcgtc gcctaccaac ggtgttactc
     3661 ggtcaacgcg aactccgcac tggacagtta aacccatctg tcgggcgtac gattcaaggt
     3721 acgtggccac ctcggcggcg gacgggtgag aatctggtgg tgcgggaaac ggcttacccg
     3781 gcaaaccggc atagcgtgcg ggcgtgaaga gcttgagcga gtcccagcga ttcctccatg
     3841 agtcgccaac agaggaatgg gcatcgacga tcacgaatct gataccccgg cgtgccaggt
     3901 gatatccggt cgcgagacct gcctggccac caccgacgat gacgacatcg acgtactcga
     3961 ggtcggtact ccgactccct acgccgtcga cggaaccgcc cgtcagtgtg acgccgtcta
     4021 cccggatgtc cctcgagggg aaggcgttgc gcgcctgtcc cttcgcctcc gtcatggctg
     4081 accctttcaa cccgcgtacg cccagtgtgt ccaataagca attacagagt gggatacctt
     4141 cccga
//
LOCUS       DASYWM010000018         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_544750, whole genome shotgun sequence.
ACCESSION   DASYWM010000018 DASYWM010000000
VERSION     DASYWM010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544750"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VGR29_00710"
     CDS             <1..288
                     /locus_tag="VGR29_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV2072133.1"
                     /translation="EASLCCGSAGIYNIIRRKMADDLGDRKAHHIAETHADQVVTANP
                     GCHMQLRTSLVRNGEEKPVRHIVEILDEAYGGKETARLRRWAIDQSPTEAP"
     gene            complement(324..2297)
                     /locus_tag="VGR29_00715"
     CDS             complement(324..2297)
                     /locus_tag="VGR29_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2072134.1"
                     /translation="MPSNVALQSLSQRLSTELVASFDPQVTSPIIGGDAMVEAFTRFG
                     QASGSAGITLEDALAVAAGALRNLFIQTGSLGTDSTADLAAGIALAAVARAHRNTGPT
                     PNSSPLGAENFPETRHARLEALYLINRAATSNLRLSEMLNTVAQVVASTTSSDASAIF
                     LYDESTGLLSLRSAVGLSQEAVGNVTVRIGNGIIGRAAIEGKLISAPDAQQHESFLAH
                     PGIGDEKYRSQVSVPILLQAQNRLVGILNIHSIERRSFDQDELEFLRTVAGELAISIE
                     NARRYSSTDERLRQKIRELGTLQRVTRSFASTLNVEGVLHLITQQAMELVRAEAAAIF
                     RPAPNKGAHDYEPIIESRIGSVRILRSVEERDSVVGEVLRTGTARHKDVSYIDGVATL
                     LCLPLHSARSTVGALCFRLQKDTELGEDTLAMLQAFSDSAAMAIENAQLYQNAMLGLQ
                     TQQTLVQEMHHRVRNNLQTVAALLSLQLRRAEDAPWAVEIREAISRIQSIAAVHDLLS
                     DERRLGGTTVDVIARMVAEDAHSTLIPPRLRVRFDIRPSELKVPSRQATIMSLLINEL
                     TANAISHGFENRDHGYIRIRAWEESGIANVEVFNDGKKVPEGFDPSVSEGLGMRITQS
                     LVTSDLKGTFTISSEEDGTVALIRFPIANDNIE"
     gene            complement(2345..>2920)
                     /locus_tag="VGR29_00720"
     CDS             complement(2345..>2920)
                     /locus_tag="VGR29_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV2072135.1"
                     /translation="PRGSAGYGEHFTLCTRARWGESDMPDVIATLEAAIAGHDWIDTD
                     RLGVTGGSYGGYLTNWIVSHDDRFKAAVTQRCVSNFYSFFGTSDIGFNFGEFEFGGVP
                     WKDAELLLRYSPISYVDQVHTPLLILHSERDLRCPIEQAEQMFTALKYLGREVAFVRI
                     PEESHDLSRSGTPSRRLARLHHLVSWFDTHL"
BASE COUNT          572 a          832 c          919 g          597 t
ORIGIN      
        1 gaggcatcgc tttgttgtgg cagcgccgga atctacaaca tcatccggcg gaagatggcg
       61 gatgacctgg gagaccgaaa ggcgcaccac atcgcggaaa cccacgccga tcaggtggtg
      121 accgccaacc caggatgtca catgcagctc cggacctctt tggtgaggaa cggcgaggaa
      181 aagcccgttc gccatatcgt tgagattctg gacgaagcgt acggaggcaa ggagaccgca
      241 aggctccggc gttgggcgat tgaccaatcg ccgacagaag ctccgtagga gagaccacgc
      301 gggtgtgaaa agtggccatg ccgctactca atgttgtcgt tcgcaatggg aaagcggatc
      361 agcgcgacgg tgccatcttc ttctgaagag atcgtgaagg tgcccttgag atcggaggtc
      421 actaaacttt gggtgatgcg cattcccagg ccctcgctca ccgaggggtc gaatccttcg
      481 ggtaccttct ttccatcatt gaacacttca acatttgcta tcccgctttc ctcccacgca
      541 cggatgcgaa tatagccgtg atcgcggttc tcaaacccgt ggctgatcgc atttgctgtg
      601 agttcgttga tgaggagcga catgatcgtt gcttgacgcg aagggacctt gagttctgat
      661 ggccggatat cgaaccggac cctgagtctc ggcgggatca gagtgctgtg agcgtcctcg
      721 gcgaccatgc gggcgatgac gtccacggtg gtcccaccca gtcgtcgctc gtcgctcaac
      781 aggtcgtgga ctgccgcgat cgactggatt cgactgatgg cctcccggat ttcaaccgcc
      841 catggcgcgt cctctgcccg ccggagttgc agcgagagga gggcggcaac tgtctgcaag
      901 ttgttcctta ctcgatgatg catctcctgg acgagggtct gctgggtctg cagtccgagc
      961 atggcgttct ggtagagctg ggcgttttcg atggccattg cggcggagtc cgagaacgcc
     1021 tggagcatgg caagcgtatc ctcccccagt tcggtgtcct tctggaggcg gaagcacagg
     1081 gccccaacgg tgctgcgggc agagtgaagc ggaaggcaca agagcgttgc cacgccgtcg
     1141 atgtagctca cgtccttgtg tcgggcagtg ccggtacgca gcacctcgcc aacgacgctg
     1201 tctcgctctt cgacacttct caggatcctg acactgccga tgcgagactc gatgattggc
     1261 tcgtagtcat gggccccttt attgggggca ggacggaaga tcgcagccgc ctcagcccgc
     1321 acgagctcca tggcttgctg ggtaataagg tgtaagacgc cctctacatt gagagttgac
     1381 gcaaaggagc gggtgacgcg ctgaagggtc cctagctcac ggatcttctg tcgcagtcgc
     1441 tcatccgtgc tgctgtatcg tctggcgttc tcgatgctga tcgccagctc tcccgcaacg
     1501 gtccggagga actccaactc gtcctgatcg aatgagcgcc gttcaatgga atggatattc
     1561 aggataccga caagtcggtt ctgtgcctgc aacaggattg gaacggaaac ctgggaacga
     1621 tacttttcat ccccaatgcc ggggtgagca aggaagctct catgctgctg cgcatccgga
     1681 gcagagatga gcttgccctc gatagcggcg cgcccgatga tgccgtttcc gatgcggacg
     1741 gtcacattgc cgaccgcctc ctgtgacagc cccacagcgg atcggaggga gagaagcccg
     1801 gtggattcgt catagaggaa tatggcgctg gcatcactgc tggtcgtact ggccacaacc
     1861 tgggctaccg tattgagcat ttcggatagt cgcaggttgg acgtggcggc gcggttgatg
     1921 agatagagtg cctccaggcg ggcgtgccgg gtttccggga agttctcagc acccaacggg
     1981 cttgagtttg gggtgggacc ggtatttcta tgggcgcggg cgacagctgc caaggcaatg
     2041 ccggcagcca ggtctgccgt tgagtcggtg ccaaggctgc cggtttggat gaagagattg
     2101 cgcagggccc cggccgctac agccaaagcg tcctcaaggg taataccggc ggatcctgac
     2161 gcctgtccga atcgggtgaa tgcttccacc atcgcgtccc cgccgatgat cggcgatgtt
     2221 acttgggggt cgaacgacgc caccagttcg gtgctcaggc gttgcgaaag gctttgcagc
     2281 gcgacattac tcggcatcgg agggtccctc aggtgggggc attcgccccc gtcgccggat
     2341 gttcctagag atgggtatca aaccagctga caaggtgatg tagccgggcc aggcggcggc
     2401 tcggcgtccc tgagcggctc aaatcgtggc tttcctccgg tatccggacg aatgctacct
     2461 cgcggccaag gtacttcagt gcggtgaaca tctgctccgc ctgctcgatc gggcaccgta
     2521 gatcccgctc gctatggagg atcaagagtg gtgtatgtac ctggtcaacg taggagattg
     2581 gactgtatcg gagcagtagc tcggcgtcct tccatggcac cccaccgaac tcgaactcgc
     2641 cgaagttgaa gccgatatcg ctggttccga agaacgaata gaagttggag acgcagcgct
     2701 gggtcactgc ggccttgaat cggtcatcgt gggacacgat ccaattcgtt aggtagccac
     2761 cgtacgagcc cccggtaacc ccgaggcgat cggtgtcgat ccagtcatgt ccagcgatcg
     2821 ccgcctccaa cgtcgcgatc acgtccggca tgtcgctttc accccaccgg gcacgtgtgc
     2881 aaagggtgaa gtgctcacca taaccggctg atccacgcgg
//
LOCUS       DASYWM010000019         3906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_357192, whole genome shotgun sequence.
ACCESSION   DASYWM010000019 DASYWM010000000
VERSION     DASYWM010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357192"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..1507
                     /locus_tag="VGR29_00725"
     CDS             446..1507
                     /locus_tag="VGR29_00725"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV2072136.1"
                     /translation="MQIEEYPVPEPEPGAVLLKMNVANVCGSDLHQWRGEFDVSKFGR
                     PYPQILGHEMTGSVFQLGEGVTADTAGQPLNVGDRVVFRYFYPCGNCPACRKGITRAC
                     PFARSYLANSCDVPPHFYGAFADYYYVRPGAAIFKVPEGLADTTVAGINCGLSQMVGG
                     LELAQLEPGEAVVIQGAGGLGVYATALAKELGARPVIVVDAIRERLELARDFGADVTI
                     DINEIPETVDRVQRVRDLTGGWGADVVVEVVGHPRVVNEGLEMLGRTGRYLEIGNVTP
                     GLTFELDPSVLVQRNITVFGMVYYEAQHLQQALERLDRTREKYPWNKVVSQTFPLEQI
                     NQAFEAADRGEVTRAAIVP"
     gene            1574..2305
                     /locus_tag="VGR29_00730"
     CDS             1574..2305
                     /locus_tag="VGR29_00730"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEV2072137.1"
                     /translation="MPTALVTGATRGIGRATALALGGIDVAANARDAEGTEAIAQAVR
                     DLGRQSLALPGDVSVPDDVGSFTRQVIETWGGVDILVNNAGITHSGSLQDTTDEAWER
                     LMDVNLKSMFLTTRALVPHMIEQRSGRIINVSSMTARLGRGFVGSASYGASKGGVVSF
                     TRGLAKELGPHNVTVNAVAPGVIVTDMNRDYLDEYGEQVLKGIPLGRFGEAEDVAGLI
                     AFLASDTASYITGAVIDVNGGLVFG"
     gene            complement(2477..3616)
                     /locus_tag="VGR29_00735"
     CDS             complement(2477..3616)
                     /locus_tag="VGR29_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2072138.1"
                     /translation="MSQFDLLLQGGDVFDPASGQRGRFDVGFVGDSLAAIEPQIDSSG
                     ARNVVQATGCLVVPGLVDVHTHVFETVGESVSADEACLTRGTTTVVDGGSAGANLFEA
                     FLRLTGGNRARVLAWLNLSTIGQVDIRVGELLALPHADVEAAVTTARLHPSLIVGLKA
                     RLSTYVVGGTAKPVLRLLREAADAAGLPIMVHVGDTGEPLPEIFEFLRPGDIVTHILT
                     GRKNGILDGAGKIIPEAFEARERGILFDASRGRNHEAFPVLQATVEQGLLPDTVSTDI
                     TRATARNRDFGLPLLATHLLSFGVPLEEVIARITLNAANAIRRGELGRLQVGGVADAT
                     LLQLEEGKFALQDVDGRIRWTDQRLVAVGTVRAGAYSERPRKEAG"
BASE COUNT          805 a         1080 c         1186 g          835 t
ORIGIN      
        1 tcgatcagat tgtccgcggt gacagccgca tcagcgggcg ggatgcccac agcttgaaga
       61 cacccgctcg caaatgttct taggacttca gcagggtaga acacctcctg gttcatatgg
      121 tctcccttca aactaacgcg gcgaacaagc aacttgatag tcgcgagcac gtctttaggc
      181 tcacatattg gagcaagcaa accacgaggc gggccgaagc tttagataat cctgcgagtg
      241 cacgatgtca agcgttgcgc tccgtattga tccattgatg aaacgtcaat tatatatact
      301 aatgtttcgc agctggttag tgtcacaaag catcgtttta gcccattgcg actgcttgtc
      361 gtcagtgggt gcagacgtaa gtgagggtgt tggctttatg gctacgatgg gacgggttgc
      421 agcattctat ggatctggaa ggccgatgca gatcgaggag tatccagtac cggagccgga
      481 gccgggagcc gtcctcctca agatgaacgt cgcgaatgta tgcggatcag acctccacca
      541 gtggcgaggt gagtttgacg tttctaaatt cggccggccg tatccgcaga ttctcggaca
      601 cgagatgacc ggttctgtct ttcaactcgg cgagggagtg actgcggaca ccgccggtca
      661 accgctcaac gtcggtgacc gggttgtctt tcgctacttc tatccctgcg gcaactgccc
      721 tgcgtgtcgt aagggcatca cccgtgcgtg tccctttgcc cgctcctatc tcgcaaatag
      781 ctgcgacgtc cctcctcact tttacggggc cttcgccgac tattactacg tgcgacccgg
      841 tgccgctatc ttcaaggtgc cagaagggtt agcggacacc actgtggcag gcatcaactg
      901 tggcttgagc caaatggtgg gaggactaga gctggcgcag ctggagccgg gcgaggcagt
      961 agtcatccag ggggcaggtg gcttgggcgt ttacgctacc gccctcgcta aggagttggg
     1021 ggcccgaccg gtgattgttg tggatgcaat tagggagcga cttgagctcg ctcgagattt
     1081 cggcgcggat gtgacgatcg atatcaacga gattcccgaa accgtcgatc gggttcagcg
     1141 ggtgcgagat ttgaccggtg gatggggggc cgacgtggtg gtagaggtgg tcgggcatcc
     1201 cagggtcgtg aatgaggggc tcgagatgct cggacggacg gggcgctacc tggaaattgg
     1261 aaatgtcacg cccggcctca cgtttgaact tgatccaagc gtgcttgtac agcgcaatat
     1321 caccgttttc ggcatggtgt attacgaggc ccagcacctc cagcaggcac tcgaacgcct
     1381 cgatcgcaca cgcgaaaagt atccctggaa caaagtcgtg agccaaacgt tcccattgga
     1441 gcagatcaat caggcttttg aagccgcgga tcgaggcgaa gtgactcgtg ccgctattgt
     1501 gccgtagtca tctcctaaac gttcgactac aaattcggtc tacggatact agcgattgag
     1561 gaggggggaa actgtgccga ctgcactcgt caccggagcc accaggggca ttggacgagc
     1621 tacggctctt gcgctgggcg ggatcgacgt ggcggccaac gcccgcgatg cggaggggac
     1681 ggaagctata gcgcaggcgg tacgcgactt gggtcggcaa tcgttggcat tgcccggtga
     1741 cgtctcggta cctgatgatg ttggatcgtt cacgcggcag gtaatcgaaa cgtggggcgg
     1801 cgtagacatt cttgtaaaca atgccggaat cacccattcc ggaagcctgc aagacactac
     1861 ggacgaggct tgggagcggt tgatggatgt caaccttaag tcaatgtttt tgacaacccg
     1921 ggcactggtg ccccatatga tcgaacagcg ctccggtcga attatcaacg tctcgtcgat
     1981 gaccgctcga ctaggccgcg gcttcgtcgg aagtgccagt tatggcgcga gcaaaggggg
     2041 cgttgtttcc ttcacccgtg gtctggcaaa ggagcttggc ccgcacaatg ttacagtgaa
     2101 tgccgtagct cctggcgtga tcgtgactga catgaaccgg gattacctcg acgagtatgg
     2161 ggagcaggta cttaagggta tcccgctcgg tcgtttcggt gaggcggaag acgtggcagg
     2221 gcttatagca ttcctcgcgt cggatacggc gagctacatc acaggggcgg tcatcgacgt
     2281 taatggcggc cttgtgttcg gataaaaggc atctcatgat tcaagtacct gctactttgg
     2341 gatagccaga cgtcccgtcg cttgggggcc acagagcaga atcgacggac cttttccgag
     2401 atcgccctag ggcggtccag gtacaccaca ctgggcgctc gcgggccagc aattgtggca
     2461 atggcaagca agacgctcag ccagcttcct tacggggacg ctcgctgtat gcgccggctc
     2521 gtacggttcc tacggcaaca aggcgctggt ccgtccaacg gattcgtccg tcaacatcct
     2581 gaagagcgaa tttgccctcc tccagctgca ggagggtggc gtcggccact ccgcctacct
     2641 gtaggcggcc caattcacct cggcggatgg cgttcgccgc gttgagtgtg atgcgggcta
     2701 tcacctcttc cagtggcacg ccgaaggaga ggagatgcgt tgccagaagc ggcaaaccga
     2761 agtcccggtt tcgggccgtg gcgcgcgtga tatccgtgct gacggtatct ggcagcaatc
     2821 cctgctccac cgttgcttgg aggactggaa acgcttcgtg gtttcgtcct cgggaagcat
     2881 cgaacaggat gcctcgttcg cgcgcctcga aggcctccgg gataatcttg ccagcgccat
     2941 cgagtatgcc gttctttcgc cctgtcagga tgtgggtaac gatatcacca gggcgcagaa
     3001 actcgaatat ctcagggagc ggttcaccag tatctcccac gtgcaccatg ataggcaggc
     3061 cggcagcgtc tgccgcttca cgcagcaagc gtagtaccgg cttggctgtg ccaccgacaa
     3121 cgtacgtgct caggcgcgct ttgagcccaa cgatgaggct tgggtgcaaa cgcgcggtgg
     3181 tcactgctgc ctccacgtca gcatgtggaa gagccagtag ctcgccgacg cggatgtcca
     3241 cttggccgat ggtcgagaga ttcagccagg caagaacgcg ggcgcggttg ccgcctgtca
     3301 ggcgtaggaa cgcttcgaac agattggctc ccgcgcttcc tccgtccacg accgttgtcg
     3361 taccacgggt gagacatgcc tcatcagcac ttacgctctc acccaccgtc tcgaatacat
     3421 gagtatggac gtcgaccagc ccaggtacta cgagacagcc agtcgcctgc actacgttcc
     3481 tcgccccgct ggagtctatt tgaggttcga tagccgccaa ggagtcaccg acgaatccca
     3541 cgtcgaaacg accgcgctgt cccgatgcgg ggtcgaacac gtcacctccc tggagcagaa
     3601 gatcgaactg gctcatttca tccccttgtt atcaattccg gctgcgtggt cctgtgggag
     3661 cgagagcggg tttgaggcaa agcttctctc actcctaccg gctagtatca cactgttttc
     3721 tgcataaccg cctgaaggag atcagcatcg gagtcgccct ttgtcccctt cttacgccaa
     3781 taggctcagg gatgaggatg aaccactgag cgaagcaatg agccatatcg gccacaaggc
     3841 tctttggaac gatgagtttt gttgcaggtg gtgtaagcgc atctggttca acatgctgga
     3901 ccatac
//
LOCUS       DASYWM010000020         9264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_207257, whole genome shotgun sequence.
ACCESSION   DASYWM010000020 DASYWM010000000
VERSION     DASYWM010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207257"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="VGR29_00740"
     CDS             <1..1578
                     /locus_tag="VGR29_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEV2072139.1"
                     /translation="RDAAFISSRACGVCGGVHSSTSALAMEMAFPVTPPPLGIAVRNL
                     MLALEFWYDNPLHLFLLAGPDYSQAIVQTTNPEIWERARTTEAPHASVHGYRTIGDIM
                     VDPNPLTGGLYLGGLQMTRVAREAYVLIGAKYPHPETVIPGGMSTTVTVSMFNEVFTR
                     LRQLFDYCKKTATIWDDICDFFYEVNPQYKKVGARPANMIDSGIWDDPFAYDASYENC
                     DAWGERRWSTPGVIVDGEVVTTKLQAMNIGIEEFIEHSYYEPWGSPRYETDPAGNPIS
                     PYHMWNKETKPQPSGQNWREKYSWSTAPRWDRRAMECECSARLWLIAAGGVMPHKRFL
                     EPTGTSLKIHVPESTMPAMELEWKIPEIWNAFERNRGRAYHLALTTVIAFENWMIGLD
                     LLKQGHTRVHTPFEPPRKGERIGVGFWGAGRGFLTHHATIDNGALSNYQILTPSTWNA
                     SPKDPWGNPGPYEEAVLNTPILEQFDDPGSFKGIDILRTIRSFDPCMPCTTHVHVDGT
                     DHVVVREVNTCGCGIDG"
     gene            1571..2110
                     /locus_tag="VGR29_00745"
     CDS             1571..2110
                     /locus_tag="VGR29_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEV2072140.1"
                     /translation="MVEEAKAHESRIGAVTRHVLIAGVGYPFLRDLSIGPVLVEGLRQ
                     LEWPAGIEVDDWSFNPIAIVQRMEERSEPYDRIVFLSGVERGREPGRVYRYRWQGELP
                     DAEEIQERIGEAVMGVISLDNLLVVCQHFKVLPPDVIVVEIEPEDTGWGAGFSLRIEA
                     ALSEVIRSIQLAAEEGVDA"
     gene            2103..2471
                     /locus_tag="VGR29_00750"
     CDS             2103..2471
                     /locus_tag="VGR29_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072141.1"
                     /translation="MHDPLDGIRCRDELLQVFYWYRGEGLGEEVTLQDVAPFLVSDSE
                     LLRDHLEDLVDEGYVERVESEPVRYRLTERGVKEGGRRFADEFAGLTGQAHGECSNPN
                     CSCQTLGPAACESRTSAHAH"
     gene            2480..3220
                     /locus_tag="VGR29_00755"
     CDS             2480..3220
                     /locus_tag="VGR29_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV2072142.1"
                     /translation="MLKVEDFVPHDDRLTDEEFRAVCQHLDELVRQFEDLPFPEVRDH
                     VFELLQMVDALHREGLGRLVGFLHDHDQGAWIDRAAEDRAVQTLFTLYDLIPMDPLRA
                     GEAPDRTGIATGRAHFIPLSQIAVSRPVRHPVLQELARMEEIPHGTMVSVDVHGKPVL
                     LANVDGEMYAVRNSCPGSVAPLSLGFFNPPVVICPWHNEAFDVRTGERVDGVQGPGLD
                     VLPISVQGGAILMAVDTVPDTQLQRSIP"
     gene            3217..3750
                     /locus_tag="VGR29_00760"
     CDS             3217..3750
                     /locus_tag="VGR29_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEV2072143.1"
                     /translation="MSRLLIAGMGNVLRGDDGFGVEAAKHLATLHSNGPDVRVIEVGI
                     GGIHLVQELMEGYDMLVVIDAVDRGSSPGTVHLLEADVPDLAEWAENERADFLADMHY
                     TTPSKALVLARALGVLPPVVYILGCQPAETDAVGIGLTSSVAQAVPKAVDVVREIMQR
                     SQDQGVDERPSSSVGMA"
     gene            3750..4076
                     /locus_tag="VGR29_00765"
     CDS             3750..4076
                     /locus_tag="VGR29_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072144.1"
                     /translation="MMDQDSRDHELEPIAPLVDYHTQEMGQVAGRLMVTLSLLTDLEN
                     SYGWDRHPDQHRETVRYARLLVSSVFRLITLTEAEQDEEMDIAKGLDEQQQRFGLGGK
                     EPNGHE"
     gene            4069..4620
                     /gene="tsaA"
                     /locus_tag="VGR29_00770"
     CDS             4069..4620
                     /gene="tsaA"
                     /locus_tag="VGR29_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEV2072145.1"
                     /translation="MNEDPCIKDRQQRSTFSVSAIGVVHRPGMSDTATTDDGTYFDPF
                     VESVVAIYPEWADGLVGIEEFSHLVVVLYMDRVKPLSQHEPIQHRVESLPGMPEVGMF
                     STRTPRRPNPLGLCYPRLLHREGNVLHVLGLDAWPGTPVLDIKGYYLRDELQPHATVP
                     DWLHRLWLKHDLERGPDHRPLNS"
     gene            4767..5003
                     /locus_tag="VGR29_00775"
     CDS             4767..5003
                     /locus_tag="VGR29_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEV2072146.1"
                     /translation="MCQAIPRKVLQVAEDSAEVLYDGKPIWIKVHDIPDLCAGEYLIV
                     YAGQALERMAAEDAEEVLESFEDIDLYFQEAIGQ"
     gene            5000..5749
                     /locus_tag="VGR29_00780"
     CDS             5000..5749
                     /locus_tag="VGR29_00780"
                     /inference="COORDINATES: protein motif:HMM:NF040399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEV2072147.1"
                     /translation="MSEILTPPQPLAGSLRFVRYAFMPNRLQFCGGDDHRMLFEYGVQ
                     QSADGGLSPLLRRFTGALPYLHLIARANRIPDPFDERVVEAYWIGNELLARVEVRQLY
                     EALQERFGKQLQGRTRDWVLGKAPAGARPHHNFHVFDVHSRVGELEHSLETMDNCRIS
                     WGKVIQVEDSELVVERQPLAMADGKLVLGPPTLERAVRQVDGRGFAEDATVGDLVSLH
                     WGWVCEVITPLQHSNLRRFTDDHIRLANQTI"
     gene            5778..6980
                     /locus_tag="VGR29_00785"
     CDS             5778..6980
                     /locus_tag="VGR29_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072148.1"
                     /translation="MRQAVISGGVAYHHGLTQPGEILFGFEVIDIGDLASVNLNQYDL
                     VLVLRSTDGDALRARQHQFGRFLDHGGVLIAFGESWSGWLPGSRWESECPEDVIEPVI
                     VSDHPLVAGWSAADLHWHPPLESWCCHGHFIAPPGAEILVQNQRGDAWLYIDRTSTNG
                     VIVASSNLDPDTHTYHGSSRARAFFARLITWARLEADLTADRRRRVAPKIAGLFSGVH
                     FQKGFYEDPEFGPRYAVLPVWELAAANLHDYAALWIPRESNQNVLMANRDKLLRYLQE
                     GGTIISFEQVNQPWLPAGAWELREVNMGSIRTGDHPIVSNLTTDDVKWHSHGAYSLYD
                     GATVLIDDSQDGVILFLDETTFAGTLIAGTLDPDCHVGFGTQRTRPLLRALVNWITQP
                     QRAAASVA"
     gene            7001..8536
                     /locus_tag="VGR29_00790"
     CDS             7001..8536
                     /locus_tag="VGR29_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072149.1"
                     /translation="MQSVTRRQVLLSLAGGAFALPIARTEARDARQSVELLQYAWSDS
                     GVPNPFQVSPAGPGGPILVSLIFDTLTWKDDKGIIPWLASEWSISSDGLTYTFRIVSG
                     ATWQDGKPLTADDVAFSFAYYARHSYVWMRSDVVESATAEGDTVRIILNRPYAAFIED
                     VTGIVPIIPKHVWESVDDPILYAGSDRSLGSGPYVLAEYNDTAGAYRLTANDAYWNGT
                     LKIAELRQLVIPPEALIQSVREGGVDLASSPDASVRDLLEDDRDLAVHESAPLSIVRL
                     AVNTTQSPLDRVEVRQAIAFALDRKLIAETITRGPAIVGSAGVVPPETPWFNPGLPQY
                     TYDPERARDLLGGERLLMNLVADVRAREPDLMIPMLEAVGITLNVQRVDPATRVQLLR
                     DGDFQLGLLTHIGVGGDPDYLRRWYSGEETNAFAQGSLFSSEEYTRLAKEQAASLDMR
                     ERKDLIFRMQEILAEELPTIVLYHRRFYWIYDPIRFTPMKTWGGLMNGIPFPHNKLAF
                     LAR"
     gene            8546..>9264
                     /locus_tag="VGR29_00795"
     CDS             8546..>9264
                     /locus_tag="VGR29_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072150.1"
                     /translation="MYHHILRLVGRYLVLVLGVVLINFLLPRMLPGDPLDFSSGDDLD
                     AAVPLTASARVQLAAYYHLDDPTLTQLRSYLIDLFVGDLGLSISRSVPVADLILARLP
                     WTLALLLVSLVFSAGMGTVVGMVAGWRPGKRRDRLLVSISGMLAAIPEFLIAMSLLLA
                     GAVGLKWFPLYGGETVFTETGRGASNSIRQALDIAWHLTLPAATLALAGISAFILVTR
                     DATAGLQNEPWLIAARAKGLN"
BASE COUNT         1836 a         2641 c         2836 g         1951 t
ORIGIN      
        1 cgggacgctg cgttcatcag cagtcgggcg tgcggagtgt gtggaggcgt gcattcctcg
       61 acgtcggcac tcgcgatgga gatggcattt cccgtcaccc cacctccgct ggggatcgcg
      121 gtgcggaacc tgatgctggc attggagttc tggtatgaca atccactcca tctctttctg
      181 ttggccggtc ctgattactc ccaagccatt gtccagacca ccaaccccga aatctgggag
      241 cgagcgagaa cgactgaggc gccacacgcg agcgtccatg gctaccgcac catcggtgac
      301 attatggtgg accccaatcc cctgactggc gggctctacc tcggtggcct acagatgacg
      361 cgagtggcac gagaagcgta tgtcctgatc ggcgccaagt acccgcaccc ggagacggtc
      421 attcccggcg gcatgagcac aacggtgacc gtgagcatgt tcaatgaggt ctttactcgg
      481 ctcaggcaat tgttcgatta ctgcaagaaa accgccacaa tctgggatga tatctgcgac
      541 ttcttctacg aggtgaatcc gcagtacaag aaggttggtg cccggcctgc caacatgatc
      601 gattccggga tctgggacga cccgttcgcc tatgacgcgt cctatgagaa ttgtgatgcc
      661 tggggcgagc ggcgttggtc cacgccgggt gtgattgtgg acggtgaggt cgtcacgacc
      721 aagcttcagg ccatgaacat cggtatcgag gagttcatcg agcattcgta ctacgagccc
      781 tggggcagcc ctcgctatga aacggatccc gccggtaacc caattagccc gtaccatatg
      841 tggaacaagg aaaccaagcc tcaaccgagc gggcagaact ggcgggaaaa atactcgtgg
      901 tcaaccgctc cgcgttggga tcggcgagca atggaatgcg aatgctcggc acgactttgg
      961 ctgatcgcgg cgggcggcgt gatgccacac aagcgctttc tcgaacccac aggcacaagc
     1021 ctgaaaatcc atgtacccga gagcacaatg ccggcaatgg agctggaatg gaagattcca
     1081 gagatttgga acgccttcga gcggaatcgt ggacgcgcct atcacctggc cttgaccacc
     1141 gtcatcgcct tcgaaaactg gatgatcggc cttgacctcc tcaagcaggg acacacgcgt
     1201 gtgcataccc catttgagcc accacggaag ggtgagcgga tcggtgttgg attctggggc
     1261 gccgggcggg ggttcctgac ccatcatgcc acgatcgaca atggagcatt gagcaactat
     1321 cagatcctca cgccgtcgac ctggaacgcc agcccgaagg acccttgggg aaatccgggt
     1381 ccttacgaag aagcagtgct caacacacca atcctggagc agttcgacga ccccggaagc
     1441 tttaagggga tcgatattct tcgaacgatc cgaagctttg atccgtgcat gccatgtacg
     1501 acacatgttc atgttgatgg caccgaccat gtcgtcgtgc gtgaggtaaa tacctgcggc
     1561 tgtggcattg atggttgagg aggcaaaggc tcacgagtcg aggatcgggg ccgtcacacg
     1621 gcatgtcctc attgctggtg ttgggtatcc attcctccgc gacctgtcga ttgggcccgt
     1681 actcgtggag ggtctgcgtc agctggagtg gccggcaggc attgaggtcg atgactggag
     1741 tttcaatccg attgctatcg tgcaacgcat ggaggagcga tccgaaccct atgaccggat
     1801 cgtgttcctg tccggagtcg agcgcggacg cgagccgggg cgggtctatc gttaccggtg
     1861 gcaaggtgaa cttccggatg cagaagagat ccaggagcgg attggcgagg cggtgatggg
     1921 tgtgatcagc ctggataacc tgctcgtcgt gtgccagcac ttcaaggtgc tgcccccgga
     1981 tgtcatcgtg gtggaaatcg agccggagga caccggctgg ggagcaggat tctcacttcg
     2041 cattgaggcg gcgctgagtg aggtgatccg gtcgatacag ctggcggcgg aggagggcgt
     2101 cgatgcatga cccactggac ggaatacgct gccgcgacga actgctccag gttttctact
     2161 ggtaccgggg agagggacta ggagaggaag tgacgctgca agatgtggcc ccgttcctcg
     2221 tttcggatag cgaattgctc agggatcacc tggaagacct ggtcgatgag ggctatgtgg
     2281 aacgtgtgga gagcgaaccg gtgcgctatc ggcttacgga gcggggagtc aaggagggcg
     2341 ggcggcgttt tgccgacgag ttcgccggcc tcacagggca ggcgcacggt gagtgcagca
     2401 acccgaactg ttcctgtcaa acccttggac cggcggcatg tgagagccgg accagcgccc
     2461 atgcgcactg aggagccgtg tgctcaaggt ggaggatttt gtgcctcatg acgaccggct
     2521 aacagatgaa gagttccggg ctgtctgtca acatctggat gagctcgtgc gccagttcga
     2581 agatttgccg ttccccgaag tgcgggacca cgtcttcgag ctcctccaaa tggttgacgc
     2641 gctccaccgg gagggactgg gtcggctggt tggattcctc cacgatcacg atcagggtgc
     2701 gtggatcgac cgtgctgcag aggatagggc cgttcagacg ctgtttacgt tgtacgacct
     2761 gattcctatg gatccacttc gcgcaggtga agcaccggac cggaccggga tcgcgacggg
     2821 aagggcacac ttcatcccac tcagtcagat cgcggtatcc cgtccagttc gccatccggt
     2881 gctccaggaa ctcgctcgga tggaagagat accgcatggg acgatggtga gtgttgatgt
     2941 ccacgggaaa ccggtgctcc tcgcgaacgt ggacggcgag atgtatgccg ttcgcaacag
     3001 ctgccctggc agcgtcgctc cgctaagcct cggattcttc aatcccccgg ttgtcatctg
     3061 tccctggcat aacgaagcct tcgatgtccg caccggggag cgagtggatg gtgtgcaagg
     3121 accaggtctc gatgtcttgc caatctcagt gcagggtggt gcgatcctga tggcggttga
     3181 taccgtcccg gatacccagc tgcagcggag catcccgtga gccgccttct catagccggg
     3241 atgggaaacg tgctgcgtgg cgacgatggc ttcggcgtag aagccgccaa gcatctggcg
     3301 accttacact cgaacggacc tgatgtccgg gtgatcgagg tcggaattgg tgggatccac
     3361 ctcgtgcagg agttgatgga gggctacgac atgctggtcg tgatcgacgc ggtcgatcgc
     3421 ggaagcagtc ctggtacggt ccatctgctt gaggccgacg tgccggactt ggcggagtgg
     3481 gccgagaacg agcgtgctga ttttcttgct gatatgcact atacgacgcc gtcgaaggcg
     3541 ctcgttcttg ccagggcgct cggcgtgctg ccaccggtgg tgtacatcct tggttgtcag
     3601 cccgcggaaa ctgatgcggt cggaatcggg ctcaccagct cagtggccca ggcggtccct
     3661 aaggcggtcg atgtggtgcg ggagataatg caacgatcac aggaccaagg agtggatgag
     3721 cgtccgtcgt catcggtagg tatggcctga tgatggacca agactctcgc gaccatgaac
     3781 tcgaaccgat cgcgccgctg gttgactatc acacccaaga gatgggtcag gtcgctggcc
     3841 ggctcatggt gacgctttca ctgctcaccg atctggagaa ttcctatggc tgggacaggc
     3901 atccggacca gcaccgggag actgtccgct atgctcgcct ccttgtctcg tctgtgtttc
     3961 gactcatcac tcttaccgaa gccgagcagg atgaagagat ggatatagcc aaggggctcg
     4021 acgagcagca gcaacgcttt ggcctcggtg gcaaggaacc aaacggtcat gaatgaagat
     4081 ccctgtatca aggaccggca gcagcgatcc accttcagtg tgtcagcaat cggcgtggtc
     4141 caccggccag gaatgagtga cactgcgacg acggacgacg gcacgtactt tgatcctttt
     4201 gtagagtcgg tcgtcgcgat ctacccggag tgggctgacg ggctcgtggg catcgaggaa
     4261 ttcagtcatc tggtggtcgt cctgtacatg gatcgagtga aaccgctcag ccaacatgaa
     4321 ccgatccagc accgcgtgga gtcgctccct gggatgccgg aagtgggcat gttcagcaca
     4381 cgcactccac gccggccgaa tcctctcggg ctctgctacc cgcgtctgct gcacagggag
     4441 ggaaacgtcc tgcatgtcct gggcctggat gcttggccgg gaaccccggt tctggacatc
     4501 aaggggtact atcttcggga cgaattgcag ccacatgcca cggtgccgga ttggttacac
     4561 cgcctctggt tgaaacatga cctggagcgt gggccagatc accggccgtt gaatagctaa
     4621 atgggatgcc ggcctgccgc cgtgctgacg ttccgtaatc ggccaacgcg ccgatcgccg
     4681 gaatagcgat cgatccggtg aacagttgga tgacgctccg ctgcactgac ggcaatgaag
     4741 atgcattcgt ggaagggtaa ccatatatgt gtcaggccat accgcgcaaa gtgctccaag
     4801 tggccgaaga cagtgcggaa gtgctttacg acggcaagcc gatctggatt aaggtgcacg
     4861 acattcccga cctgtgcgcc ggggagtact tgattgtcta cgctgggcaa gcgctcgaac
     4921 gaatggcggc cgaggatgcg gaggaggtat tggaatcctt cgaggatatc gacctctact
     4981 tccaggaagc aatcggacaa tgagtgagat cttgacgcct ccccaaccac ttgccggctc
     5041 actccgcttt gtccgctacg cgttcatgcc caaccgcttg cagttctgcg gaggggatga
     5101 tcaccggatg ctcttcgaat acggcgtcca gcagagtgcc gacgggggcc tgagccccct
     5161 cctgcgacgg ttcacggggg cgctaccgta tctccatctg atcgcgcgtg ccaaccggat
     5221 tcctgatccg ttcgacgagc gcgtcgttga agcgtactgg atagggaacg agttgctggc
     5281 gcgagtagag gtacgtcaat tgtacgaagc attgcaggaa cgcttcggca agcagctgca
     5341 gggtcgtacg cgtgactggg tgcttggaaa ggcgccggcc ggcgcgcggc cacatcacaa
     5401 cttccatgtc tttgacgtgc acagccgggt cggggagctc gagcactcgc tcgagaccat
     5461 ggataactgc cggattagtt ggggcaaggt gattcaagtt gaggattcgg agcttgtcgt
     5521 tgaacggcag ccactcgcca tggccgacgg caaactggtg cttggtccgc ccacgctgga
     5581 acgagccgtc cggcaggttg atggtcgcgg cttcgccgag gacgcaactg tcggcgattt
     5641 ggtatccctg cactggggtt gggtgtgcga ggtgattaca ccgcttcagc acagcaacct
     5701 cagacgtttt acagacgacc atattcgcct tgccaatcag acgatatagt ggtgcgatcg
     5761 cacggaggtt tcccataatg cggcaggcag ttatatccgg aggtgtggca tatcaccacg
     5821 gtctcacaca gcccggagag atccttttcg gattcgaggt tatcgacata ggcgatctgg
     5881 cgtcggttaa cctcaaccag tacgatcttg tcctcgtcct gcgatcgacc gatggcgacg
     5941 ccctccgagc acgacaacat cagtttggac gttttctgga tcacggcggc gtgctgattg
     6001 ctttcggtga aagctggtca gggtggcttc cgggctcccg gtgggaatcc gagtgcccgg
     6061 aagacgtcat cgagccggta atcgtgagcg accacccact tgtcgctggt tggtctgccg
     6121 ctgatctgca ctggcaccct ccactggaga gctggtgctg tcacgggcac ttcattgcac
     6181 ctccgggtgc cgaaatcctc gtccagaacc agcgcggcga tgcctggctc tatatcgatc
     6241 gcacgtcgac gaacggcgtc attgtcgcct catcaaatct cgacccggat acccacactt
     6301 accatggaag ttcccgtgct cgcgccttct tcgctcgtct gatcacgtgg gcgaggttgg
     6361 aggccgatct gactgccgat cgccggcgac gggtggcgcc aaaaattgct gggctctttt
     6421 ccggtgtcca cttccagaag ggtttctacg aagaccccga attcggtccg agatatgcag
     6481 tcttgcccgt ctgggagctg gccgcagcaa atctgcatga ctatgccgcg ctctggatcc
     6541 ctcgagaaag caaccagaac gtactcatgg ccaatcggga caagcttctt cgctatctcc
     6601 aagagggcgg gacgatcatt tccttcgaac aggtcaatca gccgtggttg cctgcaggag
     6661 catgggagct acgtgaggtg aacatggggt ccatccgcac cggtgatcac ccgatcgtat
     6721 cgaacctgac tacagacgac gtcaagtggc attcgcatgg agcgtattcc ctatacgatg
     6781 gagcgaccgt cctgatcgac gattctcagg acggcgtgat acttttcctg gatgagacca
     6841 cctttgccgg gacgctgatc gctggaacgc tcgatcctga ttgccatgtc ggattcggga
     6901 cacagcgaac gcgacccttg ttgcgggctc ttgtcaattg gataacacag ccccagcgtg
     6961 ccgcagcatc tgtagcctga ctacctcagc ggagacatct atgcagtccg taaccagacg
     7021 ccaggttctg ttgagtctgg cgggtggggc gttcgccctg cccatcgcaa ggaccgaggc
     7081 ccgtgacgcc cggcaatcgg tcgagttgct gcaatatgcg tggagcgact ccggtgtccc
     7141 gaatccgttt caggtttcgc cggccggtcc cggcggaccg atcctcgtca gcctcatttt
     7201 tgacacgctg acctggaaag atgacaaggg gattatcccg tggttagcgt cggagtggtc
     7261 aatcagtagc gatgggctga cctacacttt ccggatcgta tcaggtgcta cttggcagga
     7321 tggcaaaccg ctgacagccg atgatgtcgc cttctcattt gcctattacg cccgccactc
     7381 ctatgtctgg atgcggtcgg atgtggtcga atcggcaacg gcggaaggcg acaccgtccg
     7441 gatcatcctt aatcgcccct acgcggcatt catcgaagac gtcactggaa tcgtgcccat
     7501 tattcccaaa catgtctggg aatctgttga tgacccgatc ctgtacgccg gctccgaccg
     7561 ctccttgggc agtggaccct acgtgcttgc cgagtacaac gatactgcgg gcgcgtatcg
     7621 cttaaccgcg aacgatgcat actggaacgg cacactgaaa atcgccgaac tgcgccaact
     7681 tgtgatcccg ccagaggcgt tgatccaatc cgtacgggag ggaggagtag atctggcttc
     7741 aagtcccgat gcctcggtcc gagatctcct cgaggatgac agggatctgg cggtccatga
     7801 atctgcaccg ctgtcgatcg ttcgcctcgc cgtaaacacc acgcagtcac cactcgatcg
     7861 cgtggaggtg cgtcaggcaa tcgcttttgc cctcgatcgc aagctcattg ccgagacgat
     7921 tacgcgtggt cctgccattg ttggtagtgc cggtgtggtg ccacccgaaa cgccgtggtt
     7981 caatccaggc ctcccgcagt acacctacga tccggaacgc gcccgtgatc tcctcggcgg
     8041 cgagcggctt ttgatgaatc tggtggccga tgtaagggcc cgcgagccgg acctcatgat
     8101 cccaatgctt gaggcggttg gtatcacctt gaatgtccaa agagtggacc cagccacacg
     8161 tgtccaactc ttgcgcgacg gtgactttca gctcggcctc ctgacccata ttggcgtcgg
     8221 cggtgacccg gactaccttc gtcgctggta tagcggtgaa gagacaaatg ccttcgctca
     8281 gggatcgctc tttagtagcg aagagtacac acgccttgcg aaggaacaag cggcatcgct
     8341 ggacatgcgc gagcgaaagg accttatctt ccggatgcag gagattctgg ccgaggaact
     8401 tcccacgatc gtcctctatc atcgacgctt ttactggatc tatgatccca tccggttcac
     8461 tcccatgaaa acctggggag gcctcatgaa tggcattcca tttccgcaca acaagctcgc
     8521 attcctcgcg cgctgatcca gttttatgta ccatcacatc cttcgactcg tcggccgata
     8581 cttggtgctc gtgctcggcg tcgtgctgat caacttcttg ctgcctcgaa tgctccccgg
     8641 tgatccactc gacttcagtt ccggtgatga tctggatgct gctgtacccc tcaccgcatc
     8701 agcccgcgtc cagttggcgg cctactatca cctcgacgat cccaccttga cccagctacg
     8761 ctcctatctg attgatcttt tcgttggtga cttggggctc tcgatctccc gatcggtgcc
     8821 tgtggcggat ctcatcctcg cccggcttcc ctggacgttg gcgctgctgc tcgtctcgct
     8881 ggtgttctct gcgggcatgg ggacagtggt cgggatggta gcggggtgga ggcctggcaa
     8941 gcgacgcgat cgcttgctgg tctcaatatc tgggatgctc gccgccattc cagaattcct
     9001 gatcgcgatg agtttgctgc ttgcaggtgc agttgggctc aaatggtttc cgctgtacgg
     9061 cggcgaaacg gtgttcaccg agacagggcg gggggcttcc aacagcattc gacaagcctt
     9121 ggatatcgcc tggcacctaa ccttgcccgc cgcgacgttg gcgctggcag gaatctcggc
     9181 cttcattctg gtgacgcgag acgcgactgc cggcctccag aacgaaccat ggctcatcgc
     9241 cgcacgcgcg aaggggctta atga
//
LOCUS       DASYWM010000021         1860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_338765, whole genome shotgun sequence.
ACCESSION   DASYWM010000021 DASYWM010000000
VERSION     DASYWM010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338765"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VGR29_00800"
     CDS             <1..552
                     /locus_tag="VGR29_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072151.1"
                     /translation="VRGTSGAGKTTFSADLAHRLNLASIELDALYHGPHWSEPTIEEF
                     RARVRAAMDAAPEGWVIDGNYDSRLGDTVVRVADTVVWLDLPLWLKMRRLWSRTLYRT
                     RDEPELWNGNRETWRGAFLGRDSLFVYAIGAHIRHRRHWPAQFGQDPRVVRLRSDAEV
                     RRWLEQQSKPTETIGRDVSHLLP"
     gene            complement(1236..1409)
                     /locus_tag="VGR29_00805"
     CDS             complement(1236..1409)
                     /locus_tag="VGR29_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072152.1"
                     /translation="MAEKCLLLGRQRTAVMEVIDAVPQGLTVDPEITAVSHRPEALTQ
                     QGPGCLDTIAFYY"
BASE COUNT          342 a          568 c          584 g          366 t
ORIGIN      
        1 gtcaggggca cctccggggc tgggaaaacc accttctccg cggaccttgc gcaccggctc
       61 aatctcgcct ccatcgaact cgatgcgctc taccatggac cgcactggag cgagccgacc
      121 atcgaggagt tccgtgcccg cgtgcgcgcc gcgatggatg ctgctcccga ggggtgggtg
      181 atcgacggca actacgactc caggcttggt gacacggtcg tgcgcgttgc ggacaccgtg
      241 gtctggctcg atctgccgtt gtggctcaag atgcgccggc tctggagccg gaccctgtac
      301 cggacccgcg atgaacccga gctgtggaac ggcaatcgag agacatggcg tggggcattt
      361 cttggccggg actcgctctt cgtctatgcg atcggtgccc atatccgcca tcgtcgccac
      421 tggccagccc agttcggtca ggacccgcgt gttgtccggc tccgctccga cgcggaggtc
      481 cgccgatggt tggagcagca gagcaaaccg acggaaacga tcgggaggga cgtatcccat
      541 ctccttccgt gacgctgcac cgtgttcagg atctggctca acttcaccgg gcccggcgaa
      601 ctgcgctgcc ctccgagcat ctgatagccg actcggccct tctggaccgt gacgccctcc
      661 tcctggtaac cgtggacgtg cattgggatc ctgctgccgg gcttgacaac gttctcggtc
      721 tcaagacgat cgccagcagc gtcctggaca cggcgaacaa aggcaagcca ctcgtacccg
      781 ttgttgatgg tgtggatagg tgtaggtact catgctccta gcatacctgg tcctggtccg
      841 tataacgctc gaccatggca cgggtgatac ctgcgtagcc acacgcgtgt ccagccgacc
      901 agcgtcgctg ggagttcgta tcaatcctgc gttgggggcg atccacgaag gcgcgcctcg
      961 caatgtcgct tgaggtgaat ccaagatcgc ggcgtcgctg aggagggctg agaggcgcgg
     1021 tcagggcctg atcgaagcgc aatgatcggc ggcgggcatg gaaaaaccct gcccgatcca
     1081 atacgggttc aacccttatg gcggatccaa tgatctggag ctcagcacct atcgctggcg
     1141 agtcgtgggc gagaaggtgt cgattcgtgc tgcttcggtc gctccgatgt tgccacattg
     1201 ttgacacaac caatttctct ttcgcagcgc cgcagtcagt agtaaaacgc gatagtgtcg
     1261 aggcagcccg gtccctgctg cgtcaacgct tccgggcggt ggcttacggc ggtgatctct
     1321 ggatctacgg tcaggccctg cggcacggca tcgatgacct ccataacggc ggtgcgctgt
     1381 cggccgagga gtaggcactt ctctgccatg caatccgagc aggaggccaa tcggaggaag
     1441 aatcactgtg cgagcgcggg gactgcccgg acggttcact aggtgaggtg gaattcggga
     1501 agcaccttcg ccgtatccgt ttgactccac gatgaagagt gtcatccgag cggtcactaa
     1561 ggggcagcct atcaagtcgt catcgggtca gaaatggttc ataagatgct ttgtaggagg
     1621 cgttctctgt tgccgaactc aacgggagga tctcttgacc agtaccagtg ttacgctgcg
     1681 agaccagatt atggtccctc ggtatgctgg gtcctgccgc cgaggtggct cacacaggcc
     1741 agcaacgcgt ctgaggcgcc aaagctgccg gcctttgtcg ccaccggcac ggaccggacg
     1801 agctcagcgt ccagcactcc ccacggcatg gcgggacgaa tctctcctcc cagtcgcaac
//
LOCUS       DASYWM010000022        12913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_432549, whole genome shotgun sequence.
ACCESSION   DASYWM010000022 DASYWM010000000
VERSION     DASYWM010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12913
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432549"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            467..3340
                     /locus_tag="VGR29_00810"
     CDS             467..3340
                     /locus_tag="VGR29_00810"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEV2072153.1"
                     /translation="MSTSVKGKADPFGARASLSTQRGTTVGYYKLQQLVDDGVLDNLD
                     RLPFTVRILLENVLRNAGGEFTDESHVQELAKWQPTAGSAHEDFELPFLPARVVLQDF
                     TGVPCVVDLAAMRSAMDRLGGNVRKINPLVPVDLVIDHSVQVDKFGTSLAFARNVDME
                     YQRNHERYALLRWGQNAFQNFRVVPPGTGIVHQVNLEYLASVVATKQVDGDTVAMPDT
                     LVSTDSHTTMINGLGVLGWGVGGIEAEAVLLGQPLYQLSPEVVGFKFVGEMQPGTTAT
                     DLVLTVTEKLRSHGVVGRFVEYCGTGLSSLSLADRATIANMAPEYGATAGLFPVDDET
                     LNYLRLTGRDEDLVDLVERYSKAQGLFRTDETPDPEFNEMLELDLSTVEPSLAGPRRP
                     QDRVALRDVQMTFRDAFTDRIMNGKHEDQDGYDEQSAESFPASDPPAAMAGHSHSAPI
                     PEEQSVAEHKAAPPPVTLTRLGEVDIEMDGVDVPMHHGAVAIAAITSCTNTSNPSVMI
                     GAGLLAKKAVERGLDVQPYVKTSLAPGSQVVTRYLEEAGLIPYLSSLGFQTVGYGCTT
                     CIGNSGPLPEPVANIVEEKDLVVASVLSGNRNFEGRIHPQVRASFLASPPLVVAYALA
                     GTVDINLADDPLGHDPNGDAVYLKDVWPTEEEVNEAMASAVKPEMFREEYSTVFSGDE
                     RWQTLPVPEGDLYEWDEDSTYVQEPSFFQDLSPEPQPVPDLKDGRVLVMVGDSVTTDH
                     ISPAGSIPRTSPAGQYLQEHGVKPFEFNSYGSRRGNHEVMIRGTFANIRLRNLLTPDK
                     EGNWTRHLPDGEVMTIYDASQKYQEEGTPLIVIAGKEYGTGSSRDWAAKGTLLLGASA
                     VIAESFERIHRSNLVGMGVLPLQFKNGENAQSLGITGEETFTIENLDAQLAPKVTLTV
                     AATRRDGSSFTFDTIARVDSRVDVRYLRNGGILPAVLRSLLQA"
     gene            3461..4030
                     /locus_tag="VGR29_00815"
     CDS             3461..4030
                     /locus_tag="VGR29_00815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV2072154.1"
                     /translation="MEHRRIEIAALIGSTRRQSFNRGLLRTAMLLQPEDVRLIEVSIN
                     TLPFYNPDHATADDLPLVQDFRQALRRADAVLICSPEYSYSVPGILKNAMDWASVPTG
                     RSVLRGKPIGLAGASRGRSGTVRAQMHLRQILASMGAVVIPEPEVLITFAEDKFNQLG
                     ELTDHTSRELMGQLMVNLTDWAVLLRDQR"
     gene            complement(4220..5290)
                     /locus_tag="VGR29_00820"
     CDS             complement(4220..5290)
                     /locus_tag="VGR29_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEV2072155.1"
                     /translation="MESVAIPDPELSAREAGLAYVHVDRPGITRHRAGKGFFYRDPKG
                     DRITDEKTLARIISLAIPPAWENVWICPSANGHIQATGRDARNRKQYRYHPRWREVRD
                     ETKYEHMIAFGKALPSIRERVEADLRRKGMPKEKVLATIVRLLETTLIRVGNDRYAKE
                     NKSFGLTTMRDRHVDVSGSTVHFEFKGKSGVKHEIELKDRRLAAIVKRSQELPGQELF
                     QYLDEDGSRQTVSSDDVNAYLKEISGQDFTAKDFRTWAGTILAAEALTGFEAFDSEAS
                     AKKNVVQAIESVAARLGNTTTICRKCYVHPAVIDAYLEGDTVESIRQRADKELGDNLR
                     DLPPEEAAVLMLLHDRLQTAAS"
     gene            complement(5428..6321)
                     /gene="sdhC"
                     /locus_tag="VGR29_00825"
     CDS             complement(5428..6321)
                     /gene="sdhC"
                     /locus_tag="VGR29_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEV2072156.1"
                     /translation="MASVSTQRRSTATGVGFIPGSERALWYFLRVSGVILVFFALGHL
                     FITHYVNIPSDTTFDFVSARWANPLWRTFDLILLFSALWHGLVGIRLVITDLIRKQGP
                     RQIAYALLWIFGILFTILGTVTIVSFDEEMARNNTGPLSGEMWIGDLLGYSLYAFAVV
                     TYIAAVLLIIWIVRNLRAGIVPVYNGDVGQYAWVLHRATGIGILFFLLVHILDIMLIG
                     LGRDVYDHTVEFYAHWFIVPMEVMLVGAVIYHTLNGLRVIAINFSYAGAVREKKAFWW
                     AMGATILLTIPSAIIIVLAEL"
     gene            complement(6510..6827)
                     /locus_tag="VGR29_00830"
     CDS             complement(6510..6827)
                     /locus_tag="VGR29_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072157.1"
                     /translation="MNDDEWYVLQDEMTGETAGYIDRHGNTRATDPDIEDRVRNAFDR
                     ELLVRDGDVVEELGICFDGVCSIGPSDPAHDALVLRNLGALTGLRPAAELDLADGPSE
                     PAE"
     gene            complement(6820..7323)
                     /gene="moaC"
                     /locus_tag="VGR29_00835"
     CDS             complement(6820..7323)
                     /gene="moaC"
                     /locus_tag="VGR29_00835"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEV2072158.1"
                     /translation="MGRPKPGVEDLTHFDVSGRAQMVDVGGKDDTKRIAIASGRVRMS
                     PETAEVIREGRAKKGDVLGVAQVAAIMAAKRTHEIIPMCHPLMLTKINVSLEVTSDTV
                     EITAQVETTGKTGVEMEALTAVSAAGLTIYDMVKAIDRSMVIDSIQLESKEGGRSGVW
                     KRSKDDE"
     gene            complement(7324..7788)
                     /locus_tag="VGR29_00840"
     CDS             complement(7324..7788)
                     /locus_tag="VGR29_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072159.1"
                     /translation="MHEKFRLDIYTVLIIALVPFFLLTAWGLTWNYERVLASRACEDT
                     TSYLQDASRFASDFTNAGTLQNAGGWVGRMDGLRPPPLAEDLHDALVSTVTYGMNTDP
                     NLGTTQPAVVFNELTPFQNSIIEGRDKIISECPELARAIPDAYPMFFAEENS"
     gene            7897..8652
                     /locus_tag="VGR29_00845"
     CDS             7897..8652
                     /locus_tag="VGR29_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2072160.1"
                     /translation="MQHEEPLASRILLVRHAMTVVDAGRSSREWDLEEGAWRDVLSLA
                     AHLGPFNVDGIITSPERKAVATAGIIATELRLPVVADPALHEQGGEAVPWIRSERVFR
                     EAVARHFAWPTGVVLGNESSDDAVKRFGAGVQRARARYRFPLLVGHGRVMSGFIARIA
                     DTEPMEVWDARCVRCGVPEQDMATYWREGNAMTTSARLAAAISSGMIPAIVAFVVLIV
                     ADSSVNAAIVAALIIWIGLAAAMWVRFGRRGHV"
     gene            complement(8691..9563)
                     /locus_tag="VGR29_00850"
     CDS             complement(8691..9563)
                     /locus_tag="VGR29_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV2072161.1"
                     /translation="MPIVRFCPPDYGQPCLGWLAGELITPFPRNSGFTTTRQLLQHRR
                     ATITQAIQSLDNSVEPVALAEVRLLAPLDEQEIWACGVTYRRSRDARMEESTQKDVYD
                     LVYDADRPELFLKATPRRVSGPDGTITIRSDSTWDVPEPELTLVINRHHEVIGYTVGN
                     DVSSRSIEGENPLYLPQAKIYTGCAAVGPVITMPNEVPDPGRLGITLIIERDDAEYFR
                     GETSTAEIHRSLEDLVSWLHKGNSFPDGVFLMTGTGIIPPDAFTLKEGDTVRITIEGI
                     GTLGNRVALLDVNA"
     gene            complement(9642..10733)
                     /locus_tag="VGR29_00855"
     CDS             complement(9642..10733)
                     /locus_tag="VGR29_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV2072162.1"
                     /translation="MHILIQTAGASIVFIALGDIYLTVLQGGSVSWVSGRLNREVWRL
                     FRGAASILPMNRSRFLSYAGPTLVALNLITWVMLLVIGFALIFLPALGTQIVASKGDT
                     PTGFWTAIYFSAMSISTLGAGDIVATTAPYRLLEVLGVLIGFSVLTASLTYLMSIYSA
                     LIRNNAFALSLHHASTDTGKFIEVVARLGPGGDFSGGQQELSTLGMELATLIESHHIY
                     VALRYFRLASTAYALPRFAFLTIDSASLIRSAIDQERYGTIVQSTSAARLWHGGLQLV
                     TGIGGRFLPEELVDKHRPDEHDEQEWRGHFRRAANRFRDEGIVIAPDLQIAEDDYVAL
                     RREWQAHISALTDYLQYEWNEICPDEQKE"
     gene            complement(10831..11745)
                     /locus_tag="VGR29_00860"
     CDS             complement(10831..11745)
                     /locus_tag="VGR29_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV2072163.1"
                     /translation="MPKSRTPPDADVLNNDITPEADIRDDDQDAQAGEAIKEPTPMGQ
                     EAPEIVDDASKIGAKRLRRPLLGDAITAFTGGMSVSFGAVAMVTAAAAMGGGIDSPST
                     ALLVGSLVFPVGFLILLVGKTELFTENFLLPVAAVLKGRGDLRQLGSLWGTTLAFNLL
                     GALVFALLISRNHVISPGPAEEMIALATHSVNYSLWTAFIKALFAGWLMTTLTWLLVA
                     AVGFGPRIAMIWAIGALIVLGQFNHVIISAAEIFIAMLLGADITFGDWVMRNFLPALA
                     GNIFGGLVFETMLQYIQSQYHEPEEKTS"
     gene            complement(11966..12370)
                     /locus_tag="VGR29_00865"
     CDS             complement(11966..12370)
                     /locus_tag="VGR29_00865"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEV2072164.1"
                     /translation="MQNGNSFTNTSWSERFYTEQDALPRLSTVKATNTFSGAISGEGI
                     LRYVMIYTSEEAGTFTGLQTITGAVDGQRGSFTVREDGSWQGMRISSTMTVVEGSGTG
                     ALSGISGTARYTHDGGENPACPYTFDFTLDRG"
     gene            complement(12515..>12913)
                     /locus_tag="VGR29_00870"
     CDS             complement(12515..>12913)
                     /locus_tag="VGR29_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV2072165.1"
                     /translation="AKLPPGSLAATDGSHRTVTYHDSCQGLNALGLRSEPRYLIEKVM
                     GDEIVELAENSLCCGFGGSFSIEYPGVAERLMNRKLDNAQATGAPVVVTDNQGCIMHL
                     RGGADAGNRSLKVRHIAELLAERIAERYLR"
BASE COUNT         2600 a         3734 c         3914 g         2665 t
ORIGIN      
        1 gttcttctgc gcgccgggaa gaagcgcttc aaacgcgtgg cgatcgtgta gtatcagccg
       61 ttcttgagcg tcttggagtt cagcccgagg ggcttggtcg gccaattgat cccggtccac
      121 gcctctgtgc tcacagtctc ccggtgcgcc tacccatgga ggagcaggtc ttggtgattg
      181 atcgactctc gctccagctg caccgtggga gtggtcatga gccttggcca cgctcccatc
      241 gatgaccacg cgcgggggca gaccagaccc accggattct gactccaatt cattccctca
      301 tcctcatagt gccgtagaat agaggcgtat gtcacgggct tgtcaaatct tcctcgctgt
      361 cgtgcgttcg aggtcgccgc ggggcgatcg atgagcactc gttgggagag gcgaacgctc
      421 acgagttcgc tggctccggt cccggtcaga aagggttccc cataccatga gtacttcggt
      481 gaaaggcaag gccgatccgt ttggcgcgcg tgccagcttg agcacgcagc gcgggacgac
      541 tgttggctat tacaagctcc agcaacttgt cgacgatggc gttctcgata atctcgaccg
      601 cctcccgttt accgtccgca ttctgctcga gaacgtactg cggaatgccg gtggcgagtt
      661 taccgacgag tcacatgtgc aggagttggc gaaatggcag ccaacggccg ggtccgcgca
      721 cgaggacttc gagttgccgt ttcttccggc ccgggtggtg ttgcaagact tcaccggcgt
      781 gccgtgcgta gttgatttgg cggcgatgcg ctccgccatg gatcggctcg gcggtaatgt
      841 gcgcaagatc aacccgctgg tgccagtcga tctcgtgatc gatcactccg tccaggtcga
      901 caaattcggt acaagcctcg cgtttgcccg caatgtcgat atggaatacc agcggaacca
      961 cgagcggtat gcgctcctgc ggtggggtca gaacgccttt cagaacttcc gggtcgtccc
     1021 gccgggcacc gggatcgtcc accaggtgaa ccttgagtac ctcgcatcgg tcgtagcgac
     1081 gaaacaggtc gatggtgata ccgtcgcaat gccggacact cttgtcagca ccgatagcca
     1141 cacgacgatg atcaacgggc tcggtgtcct cggttggggc gtgggtggta tcgaggccga
     1201 ggcagtgctg ctcggccagc cgctctatca gctttcgccc gaggtggtcg gattcaagtt
     1261 cgtaggagag atgcagccgg gaaccacagc cactgacctc gtgctcaccg tgacggagaa
     1321 gctccggagc cacggcgtcg ttggccgctt cgtcgagtac tgcggcacgg gcctgagcag
     1381 cctgagcctg gccgaccgcg cgaccatcgc gaatatggcg ccggaatatg gcgcaacggc
     1441 aggtctcttc ccggttgatg acgagacgct gaattacctc cgactcacgg gccgagatga
     1501 ggacctagtt gacttggtcg agcgctattc caaggcgcag ggcctcttcc gcaccgacga
     1561 gacaccggat ccggaattta acgagatgct cgagctggat ttgagcactg tggagccctc
     1621 actggctggt ccacggcggc cgcaggaccg ggtagcgctg cgtgatgtgc agatgacttt
     1681 ccgcgatgcg tttaccgatc gcatcatgaa cgggaagcac gaggatcagg atggatacga
     1741 cgagcaatcc gccgagtcct tccccgcgag cgatccgcca gccgcaatgg caggtcactc
     1801 gcactctgct ccaattcctg aagagcaatc cgtcgcggag cacaaggccg caccgccacc
     1861 ggtaacgctg acgcgcttgg gcgaggtgga tatcgaaatg gatggcgtcg atgtgcccat
     1921 gcatcatggc gcggtcgcca tcgcggcgat tacctcgtgc accaacacct ccaacccctc
     1981 cgtgatgatc ggcgccggtc tgctggcgaa gaaggcggtt gagcgcggtc ttgatgtcca
     2041 gccatacgta aagaccagcc tcgctccagg ttcccaggtg gtgactcggt atctggaaga
     2101 ggccgggctc attccctatt tgagctccct gggattccag actgtcggat atggatgcac
     2161 cacatgtatc ggaaactccg gcccacttcc ggagcccgtt gccaatatcg tggaggagaa
     2221 agacctggtt gtcgcatcgg tcctctctgg caaccggaac ttcgagggac gtatccaccc
     2281 gcaggtgcgc gcgtcgttcc tggcgtcgcc gccgcttgtt gtggcgtacg cccttgctgg
     2341 taccgtcgat atcaacctgg cggacgatcc acttggacat gatccgaacg gcgatgccgt
     2401 ctacctcaag gatgtctggc cgaccgaaga agaagtcaat gaagcgatgg catctgcggt
     2461 gaagccggaa atgttccgtg aggaatacag caccgtcttt tccggcgacg agcgctggca
     2521 aacgctgccg gtgccggagg gtgacctcta cgagtgggac gaggactcga cctacgtcca
     2581 ggagccgagc ttcttccaag atctctcgcc ggaaccccag ccggtcccgg acctcaagga
     2641 cggtcgtgtg ctcgtcatgg ttggtgattc cgtcacgaca gaccatatct ccccggccgg
     2701 ctccatcccc cgtacgagcc cggcgggaca gtacctgcag gagcatgggg tcaagccgtt
     2761 cgaattcaac tcctacggct cccggcgcgg caatcacgaa gtcatgattc gcggaacctt
     2821 cgcaaatatt cgcctgcgca acctgttgac tcccgacaag gagggcaact ggaccaggca
     2881 ccttccggac ggcgaggtga tgacgatcta cgatgcgtcg cagaaatatc aggaagaagg
     2941 cacgccactc atcgtgatcg ccggtaagga gtatggaacc ggttcctccc gtgactgggc
     3001 agcgaagggg accctgctgc tcggcgccag tgcggtgatc gccgagagct tcgagcgcat
     3061 ccaccgttcc aacctcgttg gtatgggcgt actgccattg cagttcaaga acggcgagaa
     3121 cgcgcagtcg ctcgggatca cgggtgagga aaccttcacc atcgagaatc tggatgccca
     3181 gctggctccg aaagtgacac tgacggtggc cgcgacgcgt cgcgatggct cttcatttac
     3241 tttcgatacc atcgcccggg tcgatagcag ggtcgatgtc cgctatctcc gcaatggtgg
     3301 catcctgccg gcggtgctgc gcagcctgct gcaggcctag ggcagacggg cgacttggtg
     3361 caggatagcc agcggggatc ggcaaaaccg gtccccgctg acgtgagagg gagttgccgg
     3421 gggttgggtc aacgggtcgt gggaagaggg aataaacatc gtggagcatc gtcggatcga
     3481 gatagctgcg ttgatcggaa gcacgcgccg gcagtcgttc aatcgtggtt tgctgcggac
     3541 ggcgatgttg ctgcaacccg aggacgtgcg gttgattgag gtctccatca atacgctgcc
     3601 gttctataac cccgaccacg ccactgctga tgatctgcca ctcgtccagg atttccgaca
     3661 ggcgctgcgc cgggcagacg cggtgctgat ctgttccccc gaatacagtt attcagtccc
     3721 tggcatacta aaaaacgcga tggattgggc ttcggtgccc accgggcgct ccgttcttcg
     3781 tggtaagcca atcggcctgg cgggcgcgtc ccgcggtcgc tccggaacgg ttcgcgcaca
     3841 gatgcacctg cgacagatcc tcgcctcgat gggcgcggta gtgatccccg agcccgaggt
     3901 gctgattacg ttcgccgaag acaagttcaa ccagcttggg gagctcacag accatacgtc
     3961 ccgggaactg atgggacagc tcatggttaa cctgaccgac tgggcggtat tgctccggga
     4021 tcagcgctag ccaatacagg ctgaaagctt gaaatgctgg tcgtgtgccg ccgggaaagc
     4081 acagccctcg cgaagcgcct gatctgcccc tggcagggag cagatctaaa gcgaatgccc
     4141 gacggacata tcgcggatca ccttcgttgg cccgtggatg agtggatggg gcgttatgcc
     4201 gccgccttac atccgttccc tacgaggcgg cagtttggag ccggtcgtgc aggagcatca
     4261 gcactgccgc ctcttccggg ggaaggtccc tgaggttgtc gcccagctcc ttgtccgccc
     4321 gttgccggat cgattccacc gtgtcgcctt cgagataggc gtcgatcacc gccgggtgga
     4381 cgtagcactt gcggcagatc gtcgtagtgt tgccgaggcg agcggcgaca ctctcgatag
     4441 cctgcacgac atttttcttt gcgcttgctt cggaatcgaa cgcctcaaaa ccggtaaggg
     4501 cctccgcagc aaggattgtc ccggcccagg tgcggaaatc ctttgccgta aagtcctgtc
     4561 cactgatctc cttgaggtag gcgttgacgt catccgagct cacggtttgg cgcgatccat
     4621 cctcgtcgag atactggaat aactcctggc cgggaagttc ctggctgcgt ttcacgatcg
     4681 ctgccagtcg gcggtctttc agctcaattt cgtgcttcac gccggacttg cccttgaact
     4741 cgaagtggac ggtcgacccg gagacgtcga cgtggcggtc acgcatcgtc gtgaggccga
     4801 atgacttgtt ctccttggcg taccggtcgt tcccgacgcg gatcagggtc gtttccagca
     4861 gccggacgat ggtggcaagc accttttcct ttggcatgcc ttttcggcga agatccgcct
     4921 cgacacgctc ccggatcgaa ggaagcgcct tgccaaaggc gatcatgtgc tcgtattttg
     4981 tctcgtcacg aacctcacgc cacctcggat ggtagcgata ctgcttgcga ttccgcgcat
     5041 cccggcccgt tgcttggata tggccattcg cactgggaca gatccacacg ttctcccagg
     5101 cgggtggaat cgcgagggat atgatacgtg ccaacgtttt ctcgtcggta atgcggtcac
     5161 cctttgggtc gcgatagaag aatcccttgc cggcacggtg acgtgtgatg cctggtcggt
     5221 cgacgtgcac ataggcgaga ccagcctccc tggctgacaa ctcgggatcg gggatggcaa
     5281 cggattccat acgtggtgcg ttccttcaga actgtacgga ctaatctttt cgtctctgta
     5341 catgcaggtc ttgtgccaga tatatgcaaa gacggcagta ggtatagcgc ccactgccgt
     5401 ctgctactcc agattgaatt actgggctta cagttccgcg agtacgatga taatcgcgct
     5461 cggaattgtg aggaggattg tcgcgcccat tgcccaccaa aaggctttct tctcacgcac
     5521 cgcaccagcg taggagaagt tgatcgcaat cacacggaga ccattcaggg tgtggtagat
     5581 aaccgcgccg acgagcatga cctccatcgg aacgatgaac cagtgagcgt agaactccac
     5641 cgtatggtcg tagacatctc gtccaaggcc aatgagcatg atgtcgagaa tatgaacaag
     5701 caggaagaaa agaatgccga taccggtcgc acgatggaga acccatgcgt actggccgac
     5761 atcgccgttg tagaccggca caatgcctgc acggaggttc ctgacgatcc agataatcag
     5821 gagaaccgcc gcgatgtagg tgacaaccgc aaaggcgtag agcgaatagc caagcagatc
     5881 cccaatccac atctctccgg agagcggccc ggtattgttg cgcgccatct cttcgtcgaa
     5941 tgagacgata gtcactgttc cgaggattgt gaagaggatt ccgaagatcc agagcagggc
     6001 ataggcgatc tgtcgtggac cctgtttgcg gataaggtcg gtgatgacaa gacggatccc
     6061 cacaagcccg tgccagagag cggagaagag gaggatcagg tcaaacgtcc gccacaacgg
     6121 gtttgcccag cgggcagata cgaaatcgaa ggttgtgtca gacggaatgt tgacgtagtg
     6181 ggtgatgaag aggtgaccga gtgcgaagaa gaccaggatc acaccggaca cgcgcaggaa
     6241 ataccagagc gctctctctg atcccgggat aaagccgacg cccgtggcgg tgcttcttcg
     6301 ttgcgttgac accgaggcca ttgttgccgt gtcctccatc ctgtcccggt tatccaatag
     6361 ccacgagtat gggtacggct tcgcgttctg gcgctccgcg cgcatgcaca gcgtccaacc
     6421 cgcgatggcg aatcccggac gctgcgactt gcatcaactg ttggtggcaa ttgtacgatg
     6481 cgagcgctga tcagggtaac ggatccgtgt cattctgccg gttcgcttgg gccatcggcc
     6541 aggtccagct cagctgcagg tcgcaggccc gtgagggcgc caagattcct caggaccagt
     6601 gcgtcgtgag ccggatcact tggcccgatt gagcagacgc cgtcgaagca gatacccagc
     6661 tcctcgacga catcgccgtc gcggaccaat agctcgcggt cgaatgcatt tcgcacacga
     6721 tcctcgatat cgggatccgt cgctcgtgtg ttcccatgac ggtcaatgta tcctgccgtc
     6781 tcgcccgtca tttcgtcctg caggacgtac cactcatcgt cattcatcgt ctttcgatcg
     6841 tttccagaca ccggagcgac ccccttcctt ggattccagt tggattgagt caatcaccat
     6901 gctgcggtcg attgctttca ccatatcgta gatggtaagt ccggcggcac tcaccgcggt
     6961 cagcgcttcc atctccacgc cagtcttgcc ggtggtctct acctgcgcgg tgatctcgac
     7021 cgtgtcgctg gtgacctcca ggctcacatt gatcttcgtc agcatcagcg gatggcacat
     7081 cgggatgatc tcgtgcgtcc tcttggcggc catgatcgcg gctacctgtg ccaccccgag
     7141 gacgtcgccc ttcttcgcgc gtccctcccg gatgacctcg gccgtttcgg gagacatccg
     7201 cacacgaccg gaggcaatgg caatccgctt ggtgtcgtcc ttcccaccaa catcaaccat
     7261 ctgcgcacgt ccgctgacgt caaaatgggt gagatcttca acgcctggtt ttggcctacc
     7321 catctatgag ttttcctccg cgaagaacat tgggtatgcg tccgggattg cacgtgccag
     7381 ctctggacat tccgagatga tcttgtctcg tccctcgatg atcgaattct ggaagggggt
     7441 gagctcattg aagacaacgg ctggctgcgt cgtgccgagg ttcgggtcgg tgttcatgcc
     7501 ataggtgacc gtgctcacaa gcgcatcatg gaggtcctcc gcaagcggag gtggacgaag
     7561 tccgtccatg cggcccaccc atcctccagc gttttgaagt gttcccgcgt tcgtgaaatc
     7621 ggaagcaaat cgggaagcgt cctgcagata gcttgtggtg tcctcgcatg cccgggacgc
     7681 gagcacgcgc tcatagttcc acgtcagccc ccatgccgtc aggagaaaga aggggaccag
     7741 ggcgattata agaactgtgt agatatcgag gcgaaacttc tcatgcatgg gcaggctttc
     7801 atcgtatttc ggtccggcgt attgattctc atattcgtgt gccggtcgga tgccggtgcc
     7861 cggtcataat agtggaatcc ggaagaggac gggtgcatgc aacatgaaga acctctcgct
     7921 tcgagaatcc tgctggtgcg ccatgccatg actgtagtcg acgctggacg gtcctcccgc
     7981 gaatgggatc ttgaggaagg tgcatggaga gatgtcctgt ctcttgcggc acatctcggt
     8041 ccattcaatg tggatgggat cattactagt ccagagagaa aagcggttgc aacagcaggg
     8101 attatcgcca ccgagttaag acttcccgtg gtggcggatc ctgccttgca tgaacagggc
     8161 ggcgaggctg tgccgtggat cagaagtgag cgcgtgtttc gggaagcagt cgcccgccat
     8221 ttcgcttggc ccaccggggt agtacttggg aacgagtcga gtgacgatgc cgtcaagcgt
     8281 tttggtgcag gggtacagcg ggcccgtgcg cgttaccggt ttcccctgct ggtcgggcat
     8341 ggacgagtca tgagtggatt catcgcccgc atcgctgata cagaaccaat ggaggtctgg
     8401 gatgcccgat gcgttcgttg tggagttcca gaacaagaca tggcgacgta ttggagggaa
     8461 ggaaacgcga tgacgacatc ggcccgcctg gcggcagcaa tcagctcggg aatgatcccg
     8521 gccatcgtgg cctttgtagt gcttatcgtc gcggatagta gtgtcaatgc ggccatcgtg
     8581 gcggcactga tcatctggat cgggctcgcc gcggcgatgt gggtccggtt cggtaggcgc
     8641 ggccacgtct agcccatcag caaggggccg ggatggggcc gccactgcat tcaggcgttt
     8701 acatccagca gggctacgcg gttgccaagc gttccgatac cttcgatcgt gatccgcacc
     8761 gtatcgcctt ccttcagggt gaatgcgtct ggcggaatga tgccggtccc ggtcatcagg
     8821 aacacgccat cggggaagct gttgcccttg tgcagccaag agacgaggtc ttccagggag
     8881 cggtgaatct cggctgtgga cgtctcgccg cggaaatact ctgcgtcgtc gcgctcgata
     8941 atgagggtga tcccaagtcg cccaggatca gggacttcat ttggcatggt gattacgggg
     9001 ccgacggccg cgcagccggt ataaatcttg gcttgcggca ggtagagtgg gttctcgcct
     9061 tcaatagagc gggaggagac atcgtttccg acggtgtagc caatcacctc gtggtgccgg
     9121 ttgatgacga gcgtcaactc tggctccgga acgtcccagg tagaatcgct gcggatggtg
     9181 atggtgccgt ccgggccaga gacacggcgt ggcgtggctt tcaggaacaa ctctggtcga
     9241 tcggcatcgt aaaccagatc atacacatcc ttctgggttg attcctccat gcgtgcgtcc
     9301 cggcttcgac gataggtcac gccacaggcc catatttcct gttcatcgag tggtgcaagc
     9361 aagcgtacct ctgcaagagc gacaggctca acggaattgt ccaggctctg gatggcttgt
     9421 gtaatcgtcg cccgccgatg ttgcaggagc tgcctggtgg tggtgaaccc ggagttcctg
     9481 ggaaatggcg tgatgagttc cccagccagc cagccaagac agggctgacc ataatccgga
     9541 ggacaaaagc gaacgatcgg catgtgactc acctttcatg gcacaatggc gtcgatggtt
     9601 cagatgcgta catcctacgc caattctcgc aattttcggg attactcctt ctgctcgtca
     9661 ggacaaatct cattccattc gtactgcagg taatcggtaa gcgctgagat gtgcgcctgc
     9721 cattcccgcc ttaacgcgac gtagtcgtcc tcggcgatct gcagatccgg ggcgatgaca
     9781 atgccctcat cacggaagcg gttggccgcc cggcggaagt gcccgcgcca ttcctgctcg
     9841 tcatgttcat caggcctgtg cttgtcgacc aattcttccg gaagaaatct cccgccgatt
     9901 cctgtgacca gctgcagtcc tccgtgccat aggcgagcag cggatgtgga ctggacgatc
     9961 gtaccgtatc gctcctgatc aatggcggac cgaatgaggc tggcagagtc gatcgtgaga
    10021 aaggcaaagc ggggaagcgc gtaggcagtg ctggcaagcc ggaaatagcg caatgccacg
    10081 tagatatgat gcgattcgat cagcgtggca agctccatcc ccaaggtact cagttcctgc
    10141 tggcccccgc tgaagtcccc gcctggacct aacctcgcca caacctcaat gaattttccg
    10201 gtgtcggtgc tggcgtggtg aagggagagg gcaaatgcgt tgttacggat cagggcgctg
    10261 taaatcgaca tcaggtaggt gagggacgcg gtaagcaccg agaacccgat cagtaccccc
    10321 agcacctcca gcagccggta gggtgcggtc gtcgccacga tatctcccgc gccaagcgtg
    10381 gagatggaca tggcactgaa gtagatcgcc gtccaaaatc cggtcggcgt gtcacccttg
    10441 gaggcgacga tctgcgtgcc gagcgcgggc aggaagatca gcgcaaaccc gatgaccagc
    10501 agcatcaccc acgtaatcag gttgagtgcg accagggtag gaccggcata ggaaaggaat
    10561 cggctccggt tcatgggcag gatggatgcg gcgccgcgga agagccgcca aacctccctg
    10621 ttgagccgac cgctcaccca cgatacggaa ccgccttgaa gcacggtgag gtagatgtct
    10681 cccaaagcga tgaagacaat cgacgcacct gctgtctgga tcaggatatg catcgagtag
    10741 gggcctgact tctggaccgg cgagtgtcca ggtctgcatg ggagtctaga gaggaaccct
    10801 caacctcgcg gagccgggaa aatcggacaa tcagctggtc ttctcctcag gctcatggta
    10861 ctgcgactgg atgtactgca gcattgtttc gaacacgagg ccaccaaaga tgttcccggc
    10921 cagggcgggc aggaagttgc gcattaccca gtccccgaag gtgatatccg ctccgagtaa
    10981 catggcaatg aagatctcgg ctgcggaaat aatcacgtgg ttgaactgcc caagcacgat
    11041 cagtgctccg attgcccata tcatggcgat ccggggaccg aatcccaccg ccgcaacgag
    11101 aagccacgtc agcgtcgtca tcaaccagcc agcgaagagc gccttgatga atgcagtcca
    11161 caagctgtaa ttgacgctgt gcgtcgccag cgcaatcatc tcttctgccg ggccgggaga
    11221 aatcacgtgg ttccgcgaga tgagcagggc aaagacgagt gcgcccagaa gattgaatgc
    11281 cagcgtggta ccccagagcg atccaagctg gcggagatcc ccgcgcccct tgaggaccgc
    11341 ggcgacgggg agcaggaaat tctcggtgaa cagttctgtc ttgcctacca gcaggatgag
    11401 gaaaccgacc gggaaaacga gagagccaac gagaagcgcc gttgacgggg aatcaatacc
    11461 gcctcccatc gcggcggcgg cagtgaccat ggcgacagcg ccgaacgaaa cactcatgcc
    11521 tccggtaaat gccgtgatgg cgtccccaag cagggggcgt ctcaatcgtt ttgcgccgat
    11581 tttggaggca tcatcgacga tctcaggggc ttcctggccc ataggcgtcg gttctttgat
    11641 cgcttcgccg gcttgggcat cctgatcgtc atcacgtatg tccgcttccg gagttatatc
    11701 gttgttgagc acatcagcgt cgggtggtgt cctgcttttt ggcacgtagc atcgtctccg
    11761 gatgatggga ggtcgccgga tatcccggcc tgtggtactc ccaccgccag acatgtgcca
    11821 gacggggccg gtggcgctcg attcatgtca tgtgtttcat gcgttccctg aaggtggccg
    11881 aatgcggcat gccggggggt gcgcgtgacc agcacaccac ccccggcatg aggatgaaaa
    11941 gctgaacttc cgagagtcga cgtcgttatc cgcgatccag tgtgaaatcg aaggtgtaag
    12001 ggcaggcagg gttctcgccg ccatcatggg tgtagcgagc agtaccgctg atccccgaga
    12061 gggcgccggt acctgatccc tcaacgaccg tcatcgttga gctgatcctc attccctgcc
    12121 aggaaccgtc ttcacgcacg gtgaagctgc cacgctgacc atcgaccgca ccggtgatcg
    12181 tctgcaaacc ggtgaaggtt ccggcctcct cagaggtata aatcatgaca taacgcagga
    12241 tgccttcacc ggagattgct cctgaaaacg tgttcgtggc tttgacggtg gagagacgag
    12301 gcagtgcatc ctgctcggta tagaatcgct cactccaact cgtgttggta aagctatttc
    12361 cgttctgcat cgtgctgcaa tctcctgata cgaatcgaaa tgccagtgat tccacactac
    12421 cagaaaaaga acatatgttc tatacatctg gagcatgaat ttctgctgtt actaccgaat
    12481 gtggagcaga ccccggcggt ccttccgcct gcgctcagcg gaggtaacgc tcggcaatgc
    12541 gctctgccag gagttccgcg atgtgcctga ctttcagtga tcgattgccg gcgtcggccc
    12601 cgccccgaag gtgcatgatg cacccttggt tgtccgtcac gacgactggc gcgccggtgg
    12661 cctgcgcgtt atcgagcttt cgattcatca ggcgttcggc aacccccggg tactcaatgc
    12721 tgaacgagcc gccgaagcca cagcacagac tgttctccgc aagctcaaca atctcgtcac
    12781 ccatgacctt ttcaatgagg tagcgtggtt cactcctcaa gccgagtgcg ttgagcccct
    12841 gacatgaatc gtggtaggtg actgttcggt gggaaccatc ggtcgctgcc aggctaccag
    12901 gcgggagctt cgc
//
LOCUS       DASYWM010000023         7122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_76111, whole genome shotgun sequence.
ACCESSION   DASYWM010000023 DASYWM010000000
VERSION     DASYWM010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76111"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VGR29_00875"
     CDS             <1..946
                     /locus_tag="VGR29_00875"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEV2072166.1"
                     /translation="GALRCFRSATVASRHQEQGNHPDGHSVPQAEVSARNTFTRSAVV
                     EQEDFDWFTNTLATECSRRSVLRGLAGGDAIGLFTPVGTSKVGPPVVAPHYDLGVASP
                     IPLAEESLYLTDQAVGWDDWPEDDDGWTYADELLVNDGLGQGEVFLAPYQPEVTDYAV
                     EAEIRLRGATGGTSFYGFGLAARVAPEGAASFGGIMLQGKDGGPDAYVRRTGSVGPVP
                     FFQAYFEAVRDENDLSSRTAFDPGFDTWHTFRLEAERKSLRLLIDGRVTAEAEEDQTP
                     APGRVGLWSSGLQAEVRRFAVLALPEVSSATPVAKRPV"
     gene            complement(1016..1279)
                     /locus_tag="VGR29_00880"
     CDS             complement(1016..1279)
                     /locus_tag="VGR29_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072167.1"
                     /translation="MILPPYHQPPDRETGEDRWVLSRRTLLRRSVLGMTGIGLISLLA
                     ACGGDDEDVPAGLDGDGELPATDVPDVPDVEDELPDADEEGEG"
     gene            complement(1476..2570)
                     /locus_tag="VGR29_00885"
     CDS             complement(1476..2570)
                     /locus_tag="VGR29_00885"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2072168.1"
                     /translation="MAASDSVIASPDALVERLFAATLGAMDLFSVYLGGRLGYYRALA
                     DRGPATSSELAQRTGTAERYAREWLEQQAATGILTCENSDGDATERRFRLPDGYEAVL
                     VDPDSLTAMAPMAQIFAGCVKPLPQLLEAFRTGGGVPYADYGVDLHEGQAGTTQPQFR
                     HLLGQEWLPAMPDLHIRLQADPPARVADIGMGLGWSSIAIAQAYPKVRVDGFDLDEAS
                     VMAARVNAEAAGVADRVTFHARDAGAPDLVGRYDFALAVECIHDMSDPVSVLESMRRL
                     VGETGAVLVADEKVADRFTAPGDEVERFMYGFSILHCLPVGMTDQPSAATGTVMRAST
                     FRRYAREAGFNAVDELPIEHDFFRFYRLSS"
     gene            complement(2728..2886)
                     /locus_tag="VGR29_00890"
     CDS             complement(2728..2886)
                     /locus_tag="VGR29_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072169.1"
                     /translation="MGREEDIFAGTPSGTNIAAAIQLARQLGPGHTVVTVAVDFGLRY
                     LAGDLYEA"
     gene            3141..3563
                     /locus_tag="VGR29_00895"
     CDS             3141..3563
                     /locus_tag="VGR29_00895"
                     /inference="COORDINATES: protein motif:HMM:NF039977.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6069 family protein"
                     /protein_id="HEV2072170.1"
                     /translation="MSSTDFVASDNRLIMRNTMTAAVVTLALNLALYFIGDAAGWIPD
                     ELPERAEQFGLPALILSTVVPILIGGVVLVILVHTTNHPVLMFCLIASVAFIATLVAP
                     LSIAGASTAFRTFLVSLHLVTVVVGTALLVRNVADEPE"
     gene            3723..4001
                     /locus_tag="VGR29_00900"
     CDS             3723..4001
                     /locus_tag="VGR29_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072171.1"
                     /translation="MASTSIRIDRKAHERLKAIAEKEHASLSNVVEKLISRYEQEEFR
                     RAVYESFRGLREDPAEWQAYQEMVRPWDATLLDGLQNEPAFDNDDTSG"
     gene            3994..4353
                     /locus_tag="VGR29_00905"
     CDS             3994..4353
                     /locus_tag="VGR29_00905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEV2072172.1"
                     /translation="MDSSTSAVPRAGDVWYAFLDPILGHEQGGNRPVIVVSAEWFNDS
                     TGSKLVIVVPLTRTNKSYPTHVHIDRDQANLRSDSWAMCEQVRALSFERFKKKRGRVA
                     EETLAMLRMIVTQILTE"
     gene            complement(4403..5305)
                     /locus_tag="VGR29_00910"
     CDS             complement(4403..5305)
                     /locus_tag="VGR29_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV2072173.1"
                     /translation="MIRQGETHNWGRGQAWRSSSTLVLAVASVGGLAAFLYPFLLPGL
                     QQFSDENAAHSGVAPLIFAGVTVLCLVAMLVTIAEDHGGVARSKTVALLGVLVAIDAT
                     LRLVPSLGGASPVFLLIMLVGTVFGASMGFQMGALTILLSGFLTGGIGPWLPFQMLGA
                     GWMGMTAGWLPRIESPRARLVMLALFGGVWGLLFGALMNIWFWPFTAPGAGADSSLYW
                     TPGLSMAETVSRYTRFYLVTSLVFDAFRAIGNIVLVLALGGPVLRLLERYRSRFGWEP
                     WEVMEASVESGDEVPDLRQSGPIP"
     gene            5418..6884
                     /locus_tag="VGR29_00915"
     CDS             5418..6884
                     /locus_tag="VGR29_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2072174.1"
                     /translation="METTSGEITLHDLLAWEPRLTFVSPLAVPAGAADPLAREVDWVL
                     TARSSPPMLPFLRGGELIVLPQRIVQETGIPFARLVNEITMQPIAGILTEEPVPAIPD
                     SPLVILSTERIGPETESDLNRLLASRRRELLQSASEVDRLIADALERRVRPAELLDAL
                     SQRLRIPISVLTDYGAVLFSTGSGADNVPADARMSREWLSAPLKEKQTLWLGPIPTHN
                     HALMRMTLHRVRESVQRSLDRDATTAPHGTARASALNALLLPHAGLQGDSVTEQAFRA
                     GIAPGRILHVALAPGTVADSSLRRMLAPLGESHEAGHVDGYRAVITVATPAASGRGAL
                     PEIPGIPWIAISSPIDTARELPQATRQARYLAALIEASAISEPVVRFSDAARLGVYRL
                     LYDSWGSPLLERFCSTLLADLEQEDRRGLLRETLRIFLEEGGSLRPTAEALGIHRNTL
                     TYRLKQIRSIVEVDLDDPHTRLSLQIALIARQLPDAPV"
BASE COUNT         1361 a         2294 c         2169 g         1298 t
ORIGIN      
        1 cggcgcgctt cggtgtttca gatctgcaac ggtggcgtcc aggcaccagg agcaagggaa
       61 ccatccggac ggacattccg tcccccaggc agaagtatcg gcacggaaca cgttcacgag
      121 gagtgctgtt gtggagcagg aagacttcga ttggttcacc aacaccttgg cgacagaatg
      181 ctcgcgacga tccgtactgc gcgggttggc aggaggagac gccattggcc tgttcacccc
      241 ggtggggacc tcgaaggtgg gtcctcctgt cgtcgcgcca cattatgacc ttggcgtcgc
      301 cagcccgatc ccgcttgctg aggagagcct ctatcttaca gatcaggcgg tgggatggga
      361 cgactggccc gaggatgacg acggctggac atatgccgac gagctgctcg tcaatgacgg
      421 attgggccaa ggcgaggtct tcctcgctcc ctaccagcca gaggtcaccg actacgcggt
      481 ggaagccgag atccggctga ggggtgcgac cggcggcacg agcttctatg gcttcggcct
      541 tgccgcacgt gtcgcaccgg agggagcagc gtcctttggt ggaatcatgc ttcagggaaa
      601 ggatggtggc cccgatgcct atgtcaggcg gacaggcagt gtagggccgg tgcccttttt
      661 ccaagcgtac ttcgaggccg tccgggacga gaacgacctg agctcccgga ccgcattcga
      721 tcctggcttc gatacctggc ataccttccg actcgaagcg gagcggaaga gccttcgcct
      781 cctgatcgac ggtcgcgtca cggccgaggc ggaggaggat cagacccctg cgcccggccg
      841 cgtgggactg tggagcagtg gccttcaggc cgaggtgcgc cgcttcgccg tcttggctct
      901 gccggaggtt tcgtccgcga ccccggttgc aaaacgccct gtgtaacgac ctcgatgggt
      961 tgtcctgagg caccggcctg gcttctgaag ataccaggcg ctgcatcgct gcctgtcagc
     1021 cctcgccctc ctcgtcggca tccggaagct catcctcaac gtccggtacg tcggggacgt
     1081 ctgtggctgg gagctcaccg tcaccgtcta atccggctgg cacatcctcg tcgtcacccc
     1141 cgcacgccgc cagcagggat atcaggccaa tacccgtcat ccccagcacc gatcggcgca
     1201 ggagcgttcg acgagacagc acccatcggt cctcacctgt ctcgcggtcc gggggctggt
     1261 gataaggggg cagaatcatg acgtcagctc cttccaggac atgtggctca ccggtgcata
     1321 tcgggccggg gtttccgggt gccgtggcaa gaaacgcacc acctgcgcga ctagagccgg
     1381 cgtcatgacc ctcgcgcatt tacgggagat gttgccggtg tccatggggg agggcctcat
     1441 gcccatcccc agccaatcga gatgtctgtc gcccctcagc tggacaggcg gtagaaccgg
     1501 aagaagtcgt gctcgatcgg cagttcatcg acggcgttga agccggcctc cctggcatag
     1561 cgacggaagg tgcttgcccg catgacggtg ccggtggcgg ccgacggctg atctgtcatc
     1621 ccgaccggca ggcaatggag gatgctgaag ccgtacatga agcgctctac ctcatcgcct
     1681 ggtgccgtga atcggtccgc caccttctcg tcggcgacca gtaccgcccc cgtctcccca
     1741 accagccgtc gcattgattc gagcaccgag actggatccg acatgtcatg gatgcactca
     1801 acggcgagcg caaagtcata gcgcccgacg aggtcggggg caccggcgtc acgggcgtgg
     1861 aacgtcaccc ggtcggccac cccggccgcc tcagcattga cccgggccgc catcaccgat
     1921 gcttcatcga gatcgaagcc gtcgacacgc accttggggt aagcctgcgc gatggcgatg
     1981 ctcgaccagc cgagacccat cccgatgtct gccacgcggg ccggtgggtc agcctggagc
     2041 cggatatgga gatccggcat ggcggggagc cactcctgac ccagcaggtg gcggaactgc
     2101 ggctgtgtgg ttccggcctg cccctcatgg aggtcgaccc catagtcggc gtacgggacg
     2161 ccgccacccg tgcgaaacgc ttccaggagc tggggcagcg gcttgacaca gccggcaaaa
     2221 atctgggcca tcggtgccat cgccgtcagg ctgtccggat cgacgaggac cgcctcatag
     2281 ccgtccggta gccgaaagcg tcgctcggtc gcgtccccgt ccgagttctc gcaggtgagg
     2341 atgcccgtcg cagcctgttg ctccagccac tcccgggcat agcgctcggc ggtgccggtg
     2401 cgctgggcca attcgctact tgtggccgga cctcgatccg cgagcgcccg gtagtagccg
     2461 agacggccac cgaggtagac ggagaagagg tccatggcac cgagtgtggc ggcgaagagc
     2521 cgctcgacaa gggcatcggg tgaggcgatg acagaatccg atgcagccat tgctgacctc
     2581 ctgtgctgtg ccgtggtgat ggaaagtagg gagacccgtt tccatcatac acctcgcctg
     2641 acacgatccc acccgcaggg gaagggcggg tgcagacgtg gcgtcggctg ggaccatacc
     2701 tacagccctc ggtgatctgc gtgggaatca cgcctcgtat aggtcaccgg cgaggtacct
     2761 gagtccgaag tcgaccgcaa cggtgaccac ggtatgcccg gggccgagct gccgggcaag
     2821 ctggatcgcc gcggcgatgt tcgtccctga cggggtcccc gcgaagatgt cctcttcgcg
     2881 ccccatcctg ctggctatct cacgggactc atgctcctcg tgcctttgcg aaccggttta
     2941 tgcttgaagc cgcaaccgtc ttactgtgcc cgctcaaggg acatcatcga gcgagagact
     3001 agacatacca tgtaccattg aagttgtcag aaaccagcca ccaatgaatt gtcgtcggga
     3061 ttgactccac gaattggggt cgcggccaac ttctgacaac aggtagtacg ccctgaaacg
     3121 cgataagagg agggcatgac atgagctcca ctgatttcgt cgcttcagat aacagactca
     3181 tcatgcggaa tacgatgact gccgcagtcg tcaccctcgc gctcaatctt gcgctctatt
     3241 tcatcggcga cgcggccggc tggattcctg atgagcttcc cgagcgcgcc gagcagttcg
     3301 gcctccccgc gctcatcctc agcaccgttg ttcccatcct catcggtggc gtggtgctcg
     3361 tcattctcgt ccatacgaca aaccatccgg tgctaatgtt ctgcctgatc gcctccgtcg
     3421 catttatcgc gacgctggtg gccccactga gcattgccgg tgcgtcgacg gcattccgga
     3481 cgtttttggt gtcgctacat cttgttaccg ttgtcgttgg gacggcgctc ctggtcagga
     3541 acgtcgccga cgaaccggag taaccgccag cacccctgtc aggcggacaa cacagcgata
     3601 atctcgccgg gatccttgta aggacggcgc catgcaagcg cactcttaac gtgcagaccc
     3661 tgttgtgtac aatagtgcat gcaaagtagc attcatagct gcataagcaa cgaggtgtgg
     3721 tgatggcaag cactagcatt cgcattgacc gaaaagcaca tgagcgcctc aaggcaatcg
     3781 ctgaaaaaga gcacgcgtcc ttgagcaatg ttgtggagaa gttaatttcc cgttatgagc
     3841 aggaggagtt ccgtcgggcc gtctacgaaa gcttccgagg cttgcgagaa gacccggccg
     3901 aatggcaggc gtatcaggaa atggtgcgcc cctgggacgc gaccctgctt gacggcctcc
     3961 agaacgaacc tgccttcgac aacgatgaca ccagtggata gctcaacctc ggcagtgccg
     4021 cgggcagggg acgtttggta tgccttcctt gatccgattc ttggccatga gcaaggcggg
     4081 aaccggcccg tcattgtcgt ctcagccgaa tggttcaacg actctaccgg cagcaaactg
     4141 gtgatcgtgg tcccgctcac gaggacaaac aagtcatatc caacacatgt gcatatcgat
     4201 cgcgatcagg caaatctgcg atcggattcc tgggcgatgt gcgagcaagt ccgggcactg
     4261 agcttcgaac gattcaagaa gaagcggggc cgcgtggcgg aggaaactct tgcgatgctg
     4321 agaatgattg tcacgcagat attgacggaa tgatgccccg cgcatcccaa ggtctgctca
     4381 ggcaatctcg ttgattacac cgtcagggta ttgggcctga ttgacggaga tcgggtacct
     4441 catcgcccga ctcgaccgat gcctccatca cttcccatgg ctcccagcca aagcgggagc
     4501 ggtatcgctc cagcagcctg agcaccggac cacccagtgc cagcacgagc acgatgttcc
     4561 caatggcccg gaaggcgtcg aacacgagag atgtgacgag atagaaccgg gtgtaccgcg
     4621 ataccgtctc tgccatcgat agaccaggtg tccagtagag ggaggaatcc gctcccgcac
     4681 ctggcgccgt gaacggccag aaccagatat tcatgagtgc cccgaagagc agcccccaca
     4741 caccgccgaa gagcgccagc atgaccagcc gagcccgtgg tgattcgatc cggggcagcc
     4801 acccggctgt catccccatc cagccggctc cgagcatctg gaacggcaac catggtccga
     4861 tcccgcccgt caggaagccg gagagcagga tcgtcagcgc ccccatctgg aatcccattg
     4921 aggccccgaa gacggttccc accagcatga tcagcaggaa gacgggcgag gccccgccca
     4981 gactcggcac gagccgcaag gtggcgtcga tcgcgacgag tacgcccagc agtgccacgg
     5041 tcttcgaacg cgcaacccca ccgtgatcct cggcaattgt caccagcatg gcgaccagac
     5101 agagcacggt cacccccgcg aagatcaacg gcgccacccc gctgtgagcg gcattctcat
     5161 cgctgaactg ctgcaatccc ggtagcagga agggatagag gaaggctgcc agaccgccga
     5221 cgctcgcgac agccaggacc agtgtcgacg acgatcgcca cgcttgtccc ctcccccaat
     5281 tgtgcgtttc gccttggcgt atcatctgtc ccctgttccg cttcgccgga tttccggcgc
     5341 ctttccgcac ctagtatgga ctgataggat ttggaatacg ccgttcgggc cctgagggtg
     5401 agaggccaca cgattttatg gagacaacca gcggagaaat taccctgcat gacctgcttg
     5461 cgtgggagcc gcgtctgacg tttgtctcac cgcttgctgt gcccgctggc gctgccgacc
     5521 cactggcgcg tgaggtggat tgggtgctga cggcccgctc cagccccccg atgctgccct
     5581 tcctccgggg cggcgagctc atcgtcctcc cgcaacgtat cgtccaggag accgggattc
     5641 ccttcgcgcg gcttgtcaat gagatcacga tgcagcccat cgccgggata ctcactgaag
     5701 aaccagtacc ggccattccc gacagcccgc ttgtgatcct gagcacggag aggattggcc
     5761 cggagacgga aagcgacctc aaccgcctgc tggcatcacg ccgtcgcgag ttgctgcagt
     5821 cggcatcgga ggtcgatcgg ctgatcgccg atgccctgga gcgtcgcgtc cgcccggcag
     5881 agttgcttga cgcgctctca cagcgtctca ggatcccgat cagtgtcctc accgattacg
     5941 gagcggtcct cttctcgacc ggctccggtg cggacaacgt gccggccgat gcccggatgt
     6001 cccgtgaatg gctgagtgct cccctgaagg agaagcagac actctggctc gggccaatcc
     6061 ctacccataa ccacgcgcta atgaggatga ccctgcatcg cgttcgggag agtgtgcaac
     6121 gctcgctgga tcgtgatgcg accacggcac cccacgggac cgcacgggca tccgcgttga
     6181 acgccctcct cctgccccac gcaggattgc agggcgactc tgtcaccgag caggctttcc
     6241 gggccggcat cgcgccggga cgcatccttc atgttgccct cgcacctggc acggtcgctg
     6301 atagctccct ccgccgcatg ctggctcccc tgggcgaaag tcatgaggcg ggacatgtcg
     6361 acgggtatcg cgccgtgatc accgttgcca cacccgccgc ctcgggcaga ggtgcattgc
     6421 cggagatccc ggggattcca tggatcgcaa tctcatcacc cattgatacg gcacgggagt
     6481 taccacaggc tacccggcag gctcgatatc tcgccgcact gatcgaagcg agcgccatca
     6541 gcgaaccagt tgtccgcttc tccgatgcag cacgcctcgg agtctaccgg ctcctctatg
     6601 actcctgggg atcgccgctg ctcgagcggt tttgctccac gctcctcgcc gacctggagc
     6661 aggaggatcg gcggggcctg ctccgggaga cattgcgcat cttccttgag gagggaggct
     6721 cgctccggcc caccgccgaa gcgctgggga ttcaccggaa cacgctgacc taccggctga
     6781 agcagatccg gtccatcgtc gaggtcgacc tcgatgatcc gcacacacgc ctgagcctgc
     6841 agatcgcact gatcgcccgc caactgcccg acgcaccggt gtaggtagcc gtggtggaag
     6901 gccctggctc cctgcgccag cctgcaggag gccgcttggc agcaccggcc attcccaact
     6961 gaccccgccc ccattcctct acttcccaca ctgttgacat ccccaagcat atggtagatt
     7021 gtgtacgtac acatcgttcg gtcgttcttt acgacacggc atcggctggg atgccgtgac
     7081 cgaccgggag tggggaatgc acagggaaca caaacatagc tg
//
LOCUS       DASYWM010000024         9601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_507661, whole genome shotgun sequence.
ACCESSION   DASYWM010000024 DASYWM010000000
VERSION     DASYWM010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9601
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507661"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VGR29_00920"
     CDS             <1..530
                     /locus_tag="VGR29_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072175.1"
                     /translation="YMVHCGTAPVLMHIASGMYLPMIVDPAENTFEPATEVVLSQSEF
                     YVMEGEGDVYVTDTEGLFASTMTHTVMAFNGHASQYVDEPIKVPAGELCRFYVVNMGP
                     NVWSSFHIVGTIFSHGYFNANPKNALEGLQALSIGPGDGAAFEVAFPEPGTYIAVNHS
                     FGHATHGALALIEAE"
     gene            688..1278
                     /locus_tag="VGR29_00925"
     CDS             688..1278
                     /locus_tag="VGR29_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV2072176.1"
                     /translation="MEFQDVVRKRRMIRNFTNEPVSDEAIERIIEAGQHAPSAGFSQG
                     VVYVVVTREETLQVIAEIAGEEWYVRSGHAPFISGAPVQIVICTSEQVYHDRYNEPDK
                     KGKGQEEINWPIPYWHTDAGAALMLILLAAVNEGLGTAFVGVMEPKRLQDALGIPGEY
                     LPIGVTMIGHPAPDMKSGSLKRGRRPLDDVLHREHW"
     gene            complement(1534..2055)
                     /locus_tag="VGR29_00930"
     CDS             complement(1534..2055)
                     /locus_tag="VGR29_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072177.1"
                     /translation="MTEEPLYRSPQFGYAVTWTSPWGTIGSDGTGTALPESDVDGLTV
                     VMQGTELAEVRIIGADRRDLLSATETGRFWVSAAYLSEFTESGTRVVLSECCAKTAAV
                     VMVSPGEMVGTAVVTVKESYDLGPRVSIRSTFTADLAIFHEAFGSLQQSVLIDGRAPL
                     RCFDADVLLTVLT"
     gene            2724..5066
                     /locus_tag="VGR29_00935"
     CDS             2724..5066
                     /locus_tag="VGR29_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2072178.1"
                     /translation="MPDSPEVAIPSLPSGEFVGRDHELAQVISIVEQCSTRLLTIVGP
                     GGVGKTRLALTAAWELSHLFRDGTFFISFAVADKENVWELVAHTLSIPDRDREPGPEA
                     LCEAICGALREREVLLILDNCEHVIDGFADLSKLLSRCPGVILIASSQEALRLRGEQE
                     LWLRPLALPEEDHAITAGAAEHSSAVELFVLRARKVDPNFELTDDNASDIAAIVRILD
                     GLPLAIELAGAQTRHLSPAALRQRLEQDLPSLSGGPRDFPERQQSLMNMAAWSLSLLS
                     PEERDRFLQLAVLVGDFTPEIAATVVEAPSPFDGWDTLLSFADKSLIKRVAGEAQSVP
                     RFFILQTVQATTLRLLQQQPDLYVGACERHAAAYLSVAREAATHWHDREQLHWLRRME
                     QEHSNIQVILDRALTMPQLVERALELMEPMFWFWYTRGYHTWALPRIEQLLGIAPDDI
                     SPHVRGSAHVTAGWLAFEQRQVELADRHFRVGLQLLPDRASTAALWALLGTAYTLSDE
                     GKNKAAAIRQMEEVIELASTNPAASFELGAGRFGIGLLEYFDRNLPAARSRFEKALNL
                     DRACGDIQSIGMDLLYLAHIDRAGGNHRHAIHNLQQVLPIFLEVQDRANIVLGLDVVV
                     SALAGVGAFDLGWRIATVSEHIRRTRGITRAPLEQPDFEDAIHRIREGLNLDPDEPLG
                     TNQTALSLHDVTEEFLAFNLADAPIGASVTTANPRIETVLSPREMEVLQLVASGMTSS
                     EIASSLYVSQHTVKRHMANIRQKLGVRSQAAAVAVLKQAG"
     gene            complement(5309..5890)
                     /locus_tag="VGR29_00940"
     CDS             complement(5309..5890)
                     /locus_tag="VGR29_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072179.1"
                     /translation="MLTPHARRTSRSQVQGPSFRSVDEPFYKSSQFGYTVTWATPWAV
                     AESDVMDVRSPAPGTDGIAICMQDCEIAEVHIVGADRRDLLDAVETARFWASAAYLSE
                     FTESGTKVVLSQCCAKTAALVMVSPGNTPENRIVTVKESYDLGPRVSIRSTFTADLAT
                     FHEAFGSLQQSVLIDGRAPLRCFDADVLLAVLP"
     gene            6335..8686
                     /locus_tag="VGR29_00945"
     CDS             6335..8686
                     /locus_tag="VGR29_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2072180.1"
                     /translation="MALVPFTPIIGREQPIRFACNLLGEADIRLVTLTGPGGVGKTRL
                     ALEVLAVRAARGNCDVASISLAPLRDPELVLPTVATMLGVDHTGTAPLPRLIAEYVGE
                     KPLLLLLDNMEHLPAATSGIAELLASCSTLKVLATSRSPLHIKGEHIIDVPPLALPDL
                     SHLPPLAALAHIEAVELFVERARAIDPGFQINADNAASIATICARLDGLPLAIELAAA
                     RLQVLSLTSLLERLTHRLDLLTRGSRTLPERHQTLRAAIDWSYEQLPAPDQGAFERLA
                     VFAGGFDLAAAEYVVGGGATSSPLSSQAATSCQTMDALSELLENALLRREVIAGEPRF
                     MMLETIREYALERLEASGDAADAQRRHAEYFTGLAEEAEPALSIGQLSGQWPNRLEGE
                     LANLRVALTWSLSQEDPKLGLRLAAALVWFWWIRGHLVEGSEWLESALLRSREDQSAL
                     RSKLLDGAGRIARTRGEFDRAKALHNESLCIAAHLNDDVAMTRALSNLAMVIEAKGDI
                     DQATTLFERALGLARQGGQAEVLATILTNYGLMKLTHGDQQRGVALLEEGLIIARGLG
                     PSGLLGAILSNLGDARLEQGDQAASAAMYRECLILQSELGNQVGIADALLGIATIAVT
                     RGDFSFATRLLASAQALYDSIRASLSLAVMRLLDNALHEIRSVLDEDTFSAEWESGRN
                     ADLQTVIAEALELEFRPSKEMDQIPANPYYSGLTSREMQVLRLVAEGYSNREIADTLF
                     ISPQTAATHVKRLRAKIGASSRAAAAAHAHRHGLVQMGSARKA"
     gene            9035..>9601
                     /locus_tag="VGR29_00950"
     CDS             9035..>9601
                     /locus_tag="VGR29_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEV2072181.1"
                     /translation="MRISLRQYWRLLATYLKPQGKRTTVLGLLLLVSIALQLGAPQIL
                     RQFIDRASAGEPVSTLLWFAGLFLAIALVTQVVNLAEIWLAENVGWTATNNLRNDLAL
                     HVLRLDASFHNATTPGALIERVDGDVAKLGNFFSRFTVYVLGNALLGVGVLALLFRID
                     LRVGAALTLFAVLAIALINALRDVAVPHW"
BASE COUNT         1929 a         2867 c         2844 g         1961 t
ORIGIN      
        1 cctacatggt gcactgcggc accgcaccgg tgctcatgca catcgcgtct ggtatgtacc
       61 tgccgatgat cgtggacccg gcggagaaca cgttcgagcc agccaccgag gtcgtgctct
      121 cccagagtga gttctatgtg atggaaggcg agggcgacgt ctacgtcacc gacactgagg
      181 gcctgttcgc gtccaccatg acccatacgg tcatggcctt caacgggcac gctagccagt
      241 acgtcgacga gccaatcaag gttcctgccg gtgagctatg ccgcttctat gttgtcaaca
      301 tgggtccaaa cgtctggtcg tcgttccaca ttgtaggcac gatcttttcg cacgggtatt
      361 tcaacgccaa cccgaagaat gccctggaag gcctccaggc cctctccatc ggccctggcg
      421 acggtgctgc tttcgaagtc gccttccctg agccgggcac atacattgcc gtaaatcact
      481 cgtttggtca cgcaacgcat ggtgccctcg ctctcatcga ggcagagtaa tctccgaggt
      541 tctctatcag ccctggccgg atcagaatcg cgcatggttt ccggaccatg cgcgattctc
      601 acgttcaccc cggatgttcc cgtctggtag agtgcgagcc tgacggtgcc agaggttgtg
      661 gagcgacatg caggagcgag cgcagcaatg gaatttcagg atgtggtacg caagagaagg
      721 atgatccgga acttcacgaa cgagcctgta tcggacgagg cgatcgagcg catcatcgaa
      781 gcggggcagc atgcgccgag cgccgggttc agtcagggtg tggtctacgt agtggtgacc
      841 cgggaggaaa ctctccaggt aatcgccgag attgcgggcg aggagtggta cgtgcggtcc
      901 ggtcacgcgc cgtttatctc cggggcaccg gtccagattg tgatctgcac cagtgagcag
      961 gtgtaccacg accggtacaa cgaacccgac aagaagggca aggggcagga agagatcaac
     1021 tggccgatcc cgtattggca taccgacgcc ggtgccgcgc tcatgctgat cctgctcgct
     1081 gcggtaaacg aaggtctggg cactgccttt gtcggtgtca tggagccgaa acgtttgcag
     1141 gatgcgctcg ggattccagg cgagtatctc cccatcggtg tcacgatgat cggccacccc
     1201 gcgccggaca tgaaatccgg ctcactcaag cgcgggcgtc gccccctcga tgatgtgctc
     1261 caccgggagc actggtagaa cacgagcgtg agttttccag cttacgagtg gcctgatgcc
     1321 aaacgacccg ggcctactcc tgggttcggg ccaggaacgg caccaaggga ttggacggga
     1381 cgagccgggt tcgccggaca gtgcttgccg tacatcaggt caccccgcac ccggctcaca
     1441 ccgggtcaga cacatcaaca ccctcggccg ttccctgaca cggtcgaggg tgttgatgtg
     1501 tggtgcagga cggcaaggtg tcaaatggtg gtatcaggtc agcacggtca gcaagacatc
     1561 ggcgtcaaag cagcgtaacg gggcacggcc atcgatcagg acactctgct gcaagctccc
     1621 gaacgcctcg tggaagatcg ccagatcggc cgtgaaggtg gagcggatcg agaccctcgg
     1681 gcccagatcg tagctctcct tcaccgtgac cactgccgtc ccaaccattt ccccagggct
     1741 caccatcacg acagcggcgg tcttcgcgca acactccgac agcacgaccc tggtgccaga
     1801 ctccgtaaac tccgagagat acgcggcaga gacccagaac cgtcccgttt ccgtcgcgct
     1861 caggaggtct cgccgatcgg caccgatgat acggacctcc gccaattcag ttccttgcat
     1921 aacgaccgtg agaccatcta catccgactc cgggagggca gtgcctgtcc cgtcgctgcc
     1981 gatggtgccc cagggagagg tccacgtcac ggcatatccg aattggggac ttcgatagag
     2041 gggttcttcg gtcaagtggg agggacgcac cggggaccgg gaccgggagg atctgattgc
     2101 atacggattc agcattgcgg gttgcctcgc tatggttcgt agcacatatg gtgcaggatg
     2161 cagtcattta atgttttgtc cccaaatcca tgttgccagc gacatgccag ttctgtgaat
     2221 ggccccattg ggccatttcc gctgaacacc gcaatcttcc ctgacactgc ccgcgtcgcc
     2281 tgctacacgc ccagtgcagg aacagagacg gtgcattggg aacgttgggt tgaacaactc
     2341 tgttcgctga gggcttgcag atagttcccg tacgcgacgt tacatgactg ggagggaccg
     2401 acccatcggg aaagcgcggt atcgagcaac cgccacgtac ctcggcaggg aggtgaccct
     2461 ctcaccggaa gaagcaagtc tggaggggac ctgtgataat atctggttag ctatcgtaaa
     2521 ctggtaagga tacacgccag gacaaggtgg tacggagtgt ttccggttat cgagccggct
     2581 cgccagactc gtgagtagca cgccctcgac tcagatcgtt ttcatctatt tccacatcaa
     2641 ttttgtgggc ggtttatgga aatatttatc gaaaatcagt tggcttgagt cgggcactgg
     2701 tgaatgagta tggaggatga attgtgccgg acagcccgga ggttgccatt ccatccctcc
     2761 ctagcggcga gtttgttggg cgtgaccatg agcttgcgca agttatctcc atagtagagc
     2821 agtgctccac gcggcttctc accattgtcg gacccggtgg tgtcggcaaa acccgtcttg
     2881 ccttgacggc ggcctgggag ctctctcact tgttccggga tggcacgttt tttatctctt
     2941 ttgccgtagc tgataaggaa aatgtatggg agctcgttgc gcatacgctc tctatcccgg
     3001 atcgagacag ggagcctggg ccagaagcac tttgtgaagc catttgcggg gcattgcgcg
     3061 aacgcgaggt cctcctaatc ctggacaact gcgagcacgt tatcgatggc ttcgccgatc
     3121 tctcaaagtt attaagccga tgtcccggag tcatacttat tgcgtccagc caggaagccc
     3181 tgcgattgcg aggggaacaa gagctctggc tccggccgtt agccttgccc gaggaggacc
     3241 atgcgatcac cgcaggcgcg gcagaacact ccagtgcggt tgagctcttc gttcttcgcg
     3301 cgcgcaaggt agatccgaat tttgagctca cggatgacaa tgcttcagac attgccgcca
     3361 ttgtccgtat tctggatggg ctgccgctcg ccattgagct tgcgggcgcc cagacacgcc
     3421 acctttcccc ggcggctctt cgccaacgcc tggagcaaga tctgccatca ctctcaggag
     3481 gaccccgcga ctttccagag cggcaacagt cgttgatgaa tatggcggca tggagcctga
     3541 gcctgctcag cccggaggag cgagacaggt tccttcagct cgcggtgctt gtgggcgact
     3601 ttacaccgga gattgcggcc acggtggtcg aggcaccctc accgttcgat ggctgggaca
     3661 ccctgctctc attcgcagac aagagcctga tcaagcgtgt ggccggagag gcccagtccg
     3721 taccccgatt cttcatactc cagacggtgc aagcgacgac acttcggctg ttgcagcaac
     3781 agcctgatct atatgtaggc gcgtgtgaaa ggcacgccgc tgcctatctc agcgtggccc
     3841 gggaagcggc gactcactgg catgatcgcg agcagttgca ctggctccgg cgcatggaac
     3901 aggaacactc caatatccag gtcattctgg accgcgccct gaccatgcct caactggtag
     3961 agcgtgccct ggagttgatg gagccaatgt tttggttctg gtacacgcgt ggctatcaca
     4021 cgtgggcctt gccacgtatt gagcagcttc ttggaatcgc accggacgat atctctcccc
     4081 acgtccgggg ctcggctcat gtcaccgcag gatggctggc gttcgagcag cgacaggtgg
     4141 agttggcaga tcggcacttt agggtaggtc tccaactctt gccagaccgt gcgtcaactg
     4201 ccgctctctg ggcactcctg ggtacggcct acaccctatc ggacgagggc aagaacaagg
     4261 ctgcagcgat ccgacagatg gaagaggtga tagaactcgc ttcaactaat ccggccgcat
     4321 cgttcgaact tggcgccggg cgcttcggca tcggtttgct ggagtacttc gaccggaacc
     4381 ttccagcggc caggtcccgt ttcgagaagg cactcaacct cgatcgggca tgcggtgaca
     4441 tccagagcat cgggatggac cttctctatc tcgcgcatat cgatcgcgct ggcggaaatc
     4501 accgtcatgc catccataac cttcagcaag tactcccgat cttcctagaa gttcaggatc
     4561 gggccaacat tgtgttgggc ctcgacgtcg ttgtttctgc tttggccggg gttggtgcgt
     4621 tcgaccttgg ctggcgcatc gcgaccgtga gtgagcacat tcgccgcacc aggggcatca
     4681 cgcgagcacc gctcgagcaa cccgattttg aggatgcaat tcatcgcatc cgggagggtt
     4741 tgaacctgga cccggacgaa ccgcttggaa cgaatcagac agcactatcg ctgcacgacg
     4801 tcaccgagga atttctggcg ttcaacctgg cagacgctcc catcggtgcc agtgtgacga
     4861 ctgccaaccc acgaattgag accgttctct ctccacgaga gatggaggtc ctgcagctcg
     4921 tcgcgtccgg aatgacctca tccgagatcg ccagttcgct ctacgtaagc caacacaccg
     4981 tgaagcgcca tatggcgaac atacggcaga agctgggtgt gcgctcccag gccgccgcgg
     5041 tcgccgtcct gaagcaagcc gggtaagaga tacggtgcga tctctcacac gtctgattgg
     5101 acgaaccagt cgtgaaggtc atgccactga ctggtactca acaagatggt gcctggtggc
     5161 ggcacaatgg cgacacacca gatatctgcg aggcaggtgg aacatccctg aactgctcaa
     5221 ttcactaatt tcgccgaagt ccccggcatc aatgggggac aaccggtgac ttcggcaaga
     5281 gtggtgagca agaagttaaa tcaaggtatc agggcagcac ggccagcaag acatcggcgt
     5341 caaagcagcg taacggggca cggccatcga tcaggacact ctgctgcaag ctcccgaacg
     5401 cctcgtggaa ggtcgccaga tcggccgtga aggtggagcg gatcgagacc ctcgggccca
     5461 gatcgtagct ctccttcacc gtgacaatcc gattctccgg agtgttgccc gggctgacca
     5521 tgacgagagc cgcggtcttg gcacagcatt gggacagcac gaccttggtg ccagactccg
     5581 taaactccga gagatacgcg gcagaggccc agaaccgagc agtctctaca gcatcaagga
     5641 ggtctcgccg atcagccccg acgatgtgca cctcggcgat ctcacagtct tgcatacaga
     5701 tcgcgattcc gtcggttccc ggagcaggac tgcgtacatc catgacgtca ctttcggcaa
     5761 cggcccaggg agttgcccag gtaacggtat agccaaactg ggaactttta tagaagggtt
     5821 catcgacgga tcggaacgaa ggtccctgta cctgggaccg ggaggttcgg cgagcatgcg
     5881 gagtcagcat tgcgggttgc ctcactcagg tagatagctg ggggcatgat gcagagatgt
     5941 gtcgtcccca aagctctgat gatgatagtg acacgctgct tctatttgga atccaatata
     6001 tggagtgtat tggcctacgt accggatcgt ctgtaccccg aattgggtaa ttcgatgggc
     6061 acctacgcga ggtacttacc caccgtggaa gagtcggtgc cacgcttcgc aggttggtgg
     6121 aacaggtcaa ctccaggatg agcaagtaac cggacaggat ccgggtctcc tcattgggtc
     6181 atgaaatgcg cttttgaagg ggcctgaagg tccgccaaac gactgtagcg ttgacaaacc
     6241 agtaagcctg ccacacaatc caagcatctt acatgtcaca acgtcgttga cagagggcag
     6301 cgtgtccgtc ttgccaaacc tggtccacac tcgcatggca ctggtaccct tcactccgat
     6361 tatcggccgg gaacagccga tccgtttcgc gtgcaatctg ctcggcgagg ctgacattcg
     6421 cctggtcacc ttgacaggac cgggtggtgt aggcaaaacc cggcttgccc tggaagtcct
     6481 tgcagttcgc gccgcgcggg gtaattgcga tgtggcgagt atctccctgg cccctctccg
     6541 tgacccggaa ctggttctac cgacagtcgc caccatgctc ggcgtggacc ataccggcac
     6601 tgcaccgctt cccaggctca tcgccgaata tgtgggcgaa aagcctctgc tgctcctgct
     6661 ggataacatg gagcatcttc ccgcagccac gtcagggatc gcggagctgc tggcgtcatg
     6721 ttccaccctg aaggtgcttg caaccagccg cagcccgctt catataaagg gtgagcacat
     6781 tatcgacgtg ccgcctcttg cattacctga tctgtcgcac ctccctcctc tcgcagcgct
     6841 tgcacatatt gaagcggtcg agctttttgt ggagcgtgcc cgcgccattg atccgggctt
     6901 tcagatcaac gctgacaacg cggcttcgat tgcgacgatc tgtgcccgtc tcgatggact
     6961 gccattggcc atcgaactgg ccgcggcgcg gctccaggtg ctctcgctca cgtcactcct
     7021 cgagcgtctg acgcatcgcc tagacctgct cacgagggga agccgcacgc tccccgagcg
     7081 acaccagacg ctgagggccg ccatagactg gagctacgag caacttccgg ctccggatca
     7141 gggggcattt gagcgcctcg ctgtgttcgc gggcggtttc gaccttgcag cagctgaata
     7201 tgtggtcggc gggggggcga catcgtcccc tctttcatcc caagcagcca cttcttgcca
     7261 gacgatggac gccctttccg agctcttgga gaatgcgctg ctcaggcgcg aggtgatcgc
     7321 tggagaaccg cggttcatga tgctcgaaac gatccgggag tatgcactcg aacggctcga
     7381 ggcgagcggc gacgcagcag acgcccaaag gcgacatgcg gagtatttca ccggtctcgc
     7441 ggaagaggct gaaccagcat tatcaatcgg gcaattgtcc ggccagtggc caaaccggct
     7501 ggaaggagaa ctcgcgaatc tgcgggtggc cctcacatgg tcgctcagcc aggaggatcc
     7561 caagcttggg cttcgcctcg ccgccgcgct ggtctggttc tggtggattc gcggtcatct
     7621 tgtcgaaggc agcgagtggc tggagtccgc gctcttgcgg tcgcgtgagg atcagtccgc
     7681 cttacgctca aagctccttg atggcgccgg gcggatcgcg agaacccgag gggagttcga
     7741 ccgtgcgaaa gccctgcaca atgagagcct gtgcatcgct gctcacctga atgatgatgt
     7801 cgcgatgaca cgagccttgt cgaatctcgc aatggttatt gaagccaagg gtgacatcga
     7861 tcaggcaacg acgctcttcg aacgcgcgct cgggctggca cggcaaggcg gccaggccga
     7921 ggtactcgcc acaatcctga ccaattatgg cttgatgaag ctcacccatg gggaccagca
     7981 acgaggcgtt gcgctgcttg aggaggggct catcatcgca cggggactcg ggccgtccgg
     8041 actcctgggc gctattctgt ccaatctcgg tgatgctcgt ctggagcagg gggatcaggc
     8101 cgcgtcagca gcaatgtacc gggaatgcct gatcctgcag tccgagctcg ggaaccaggt
     8161 cggcatcgcg gatgccctac tcggcattgc caccatcgcc gtaaccaggg gagacttctc
     8221 gttcgcgacc cgtctgcttg catcagcaca agccctctac gactcgatca gggcatctct
     8281 ttcacttgcg gtcatgcgat tgcttgacaa cgccttgcat gagatacggt cggtcctgga
     8341 cgaggacacc ttttccgcgg aatgggaatc gggacgaaac gcggatctgc aaaccgtcat
     8401 tgcggaagcg ttggaactcg agtttcgtcc atccaaagaa atggatcaga tccccgccaa
     8461 cccgtactat agtggcctca cgtcgcgcga gatgcaggtt ctgcgcctgg tggctgaggg
     8521 ctactccaac cgagagattg cggatacgct ctttatcagc ccacagaccg cggcaacgca
     8581 cgtcaagcgg ctccgggcca aaatcggagc ctcctcacgg gctgccgcgg ctgcccacgc
     8641 ccatcggcac ggcctcgtcc agatgggttc tgcacgaaag gcgtgaaatt gcgcccgtcg
     8701 gcttcgccgc tctggctcat caatgggcgt atcgacaaca ctgtccagca cgacggcaca
     8761 gatcccagga aacgataagc agtcagggct ccatcccgct ggtcattcaa cggattcggt
     8821 ctccatcggc ccacatattg tgaagcgaca tcgggcgaat attcggccgg agcctggcgc
     8881 gtgttcccgg ctgcagccgc ggctgcgtta cagcaatagc ctctgctttt tccggtgctg
     8941 ccccaccaga cacagtgaat atgggccgtg ctacagtggg ttgcctgctt cttgcttgag
     9001 cacctgaaag tacgtttccg gagcacactc aaacgtgcgg atctccttgc gccagtactg
     9061 gcgcttgctc gcaacctacc tgaagcccca aggcaagcgc acgaccgtcc tgggcctgct
     9121 gctgctcgtc agcattgcgc tgcaacttgg ggcacctcag atcctccgcc agttcatcga
     9181 ccgcgcctcc gcgggcgagc cggtgagtac cctcctttgg tttgccgggc tcttcctggc
     9241 cattgccctc gtcacgcagg tggtcaatct cgctgaaata tggctggcgg agaatgtcgg
     9301 atggaccgcc acgaacaacc tccgcaacga tctcgcgctg cacgtgctga ggctcgatgc
     9361 atcatttcac aacgcaacca cacctggcgc gctcattgag cgggtggatg gtgatgtcgc
     9421 taagctgggt aacttcttct cgcgtttcac ggtctatgtt ctcggcaatg ccctgctcgg
     9481 cgtcggagtg ctggccctgc tctttcgcat tgacttgcgg gtcggcgcag cactgaccct
     9541 ctttgccgtt ctggcgatcg ccctcatcaa tgcactgcgc gacgttgccg tccctcactg
     9601 g
//
LOCUS       DASYWM010000025        15972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_395170, whole genome shotgun sequence.
ACCESSION   DASYWM010000025 DASYWM010000000
VERSION     DASYWM010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15972
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395170"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VGR29_00955"
     CDS             <1..588
                     /locus_tag="VGR29_00955"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV2072182.1"
                     /translation="QFDLPPFLGLGFGFGVVGVLFSLMGQRAQHPHGEVEGGTAPLTR
                     MEHDIQEVLREVDYSSLIFFVGILLSVAALDSLGVLAEVSQTLFGAVPSFSRVAAGSI
                     LMGWGSAIVDNVPLTAVAIDTIPTSDSSLWSLLAYTVGTGGSHLIIGSAAGVVVMGRV
                     NALTSLAYMRIAALPVLVAYLSGVVIWVAQILVFG"
     gene            complement(793..1614)
                     /locus_tag="VGR29_00960"
     CDS             complement(793..1614)
                     /locus_tag="VGR29_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEV2072183.1"
                     /translation="MERTNPNGNLRIGLAAARNAPSVEERLQIVDRFLGEAAAREVAI
                     VCFPEAYIPGLRGQDVEVPPHDQVRQDAALEEIRASAQRHGVAVVIGMEWETTLGLHN
                     VAFVVSRDGVIEGYQAKNQIPPEEEPFYVPDGRRRLFEVDGVPFGITICHEGWRYPEA
                     VRWSAVRGARVVFHPQVTGSDRTGVTIDRWGDPASPYYEKAMVARSVENTIYFASVNN
                     AMRYQDSATSLIDPDGVCVAHVPYGQEQLLVHDLDLSRATGFCARRFNPAFYPAT"
     gene            complement(2027..3109)
                     /locus_tag="VGR29_00965"
     CDS             complement(2027..3109)
                     /locus_tag="VGR29_00965"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEV2072184.1"
                     /translation="MARQTSPQAVTRRRFMGIAGLTGAGAIGTALSPVNVRIEREPAA
                     LAQSTDHDHEKVASAQTTSKKKDEPTADEMDAMHEAGIKSFPAKTEGLGGQPLEYEMD
                     GDVKVFSLTCQVTNWEFAPGEFVDAWTYNGVVPGPEIRVTEGDKVRFEVTNELPQSTA
                     VHWHGLVVPNNQDGVPFITQPPIKPGETYTYEFQIRDGNAGTHMYHSHHNSAFQVTQG
                     LLGSFIVEPKDPSSRPAFDREYTIVLNDGPIGGFSLNGKSFPATQPLMAKKGEKILVR
                     YMNEGLMIHPMHLHGMPMTVTAADGYLLPQPYLCDALNIAPGQRFEAIIDATEPGTWA
                     FHCHILSHAESEHGMFGMVTALIVEE"
     gene            complement(3439..3852)
                     /locus_tag="VGR29_00970"
     CDS             complement(3439..3852)
                     /locus_tag="VGR29_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072185.1"
                     /translation="MAQLSPGKGGNGGPASDQPGIEREELLHVAVIAAIFLPNNGRRI
                     GVDSVEGGAQGEWGSQAMATQPKHPGGDSHRGNNCEGDDLTANELILDVGGRAERNGL
                     EDKRAKDCARGNPGTKRNRANARKAGPNHHPTPFG"
     gene            3891..4337
                     /locus_tag="VGR29_00975"
     CDS             3891..4337
                     /locus_tag="VGR29_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072186.1"
                     /translation="MAGIFLGLGSFFALAWAQQIRLVYIGSATAEYQEGPTIFWLIKL
                     LDFGFMIPLLLATGVGLIRRNRTAIKAAYGLATFATCLVGSIIGMAVVMQAKGDPSAQ
                     PVMLVVLIPATIGLALVTEHLLRSYVQGTRERPEFSATPRVGWEMG"
     gene            complement(4529..5254)
                     /locus_tag="VGR29_00980"
     CDS             complement(4529..5254)
                     /locus_tag="VGR29_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2072187.1"
                     /translation="MRKQLVLVVDDEASIVRLLRATLEADGFGVATADRGPTALNIIN
                     TERPDLVVLDVMMPEMDGFETLRRIRSQSQVPRVPVIMLTARTGDIDKLQGFQSGADD
                     YVTKPFNPDELLARITAVLRRSGGDSPATTNQMLRYPDLEIDLDQRRVLVRGEEIRLS
                     RTEWALLSQLAGNPGRIMLHAELLTRIWGPEFRDEVHYLRTWVSRLRAKLEPDAGDES
                     IISTFPGVGYRLEPPGGSPARQE"
     gene            complement(5331..6986)
                     /locus_tag="VGR29_00985"
     CDS             complement(5331..6986)
                     /locus_tag="VGR29_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2072188.1"
                     /translation="MAQGYGSVNGQTPSDESSKSSPPNLAVVAAYIAALDVASEVQLS
                     VVLQRLVDLARDVIPSKYAALGVSDEHGHITEFITSGISSLERARIGPIPQGHGMLGA
                     LIRDRSPMIVPNIGEDPRSVGFPPHHPPMHSLLGVPLLIGDRVVGNLYLTERLENRNY
                     DESDLQAIQILAAHAATAIDRAQLFSEVGDSRRRAEEQRDQLRVILDSLPSGVIIQTA
                     PEGEIELDNATAREMVSPRPGSDTPGRHYHFLRADGSPLPRIQWPAMRALRGEVVRNR
                     QLTLRIGDDRTVPVLVQAVPLRNRLGEVVRAVVVFQDVTRLREAEQLKDDFLSLISHE
                     FRTPLTAIHGGAHLLAQRGETLDRETQQALLTDIVTESTRLEQMLANMLTLTAIQAGR
                     LEPGTEPLLVPPLVRGASATAEAKSRDHRFVVDISNSVPPVEGDAELLNQVLRNLYEN
                     AAKYSPPGSTITTYVTCDNGSITIHVADEGAGIATEHLGTVFERFRRPGADPTVRGMG
                     LGLYLSRHLIEAQGGQIRVSSPGPGKGATFSVTLPVSSDWSDT"
     gene            complement(7022..7552)
                     /locus_tag="VGR29_00990"
     CDS             complement(7022..7552)
                     /locus_tag="VGR29_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2072189.1"
                     /translation="MYQRIVVPLDGSDLAERALVEAETLATLTHAPIHLVRVIDGTAD
                     HTAAPYTAMLNPLALSASLADEIDSVRQYLGSAAQRIAARGYEVSHELRHGQVVDQLI
                     AVSKPGDLLVMASHGRSGISRWFMGSIAEDLVRRVSIPVLLVKASSYTGSGKGSGRTL
                     AGAMTGETAMTATRSW"
     gene            complement(7933..8262)
                     /locus_tag="VGR29_00995"
     CDS             complement(7933..8262)
                     /locus_tag="VGR29_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072190.1"
                     /translation="MTTFEDISHHPNILLEHADDGVVSPLGVLFLLAGLTFIGAGIGV
                     LRRAPWWPPAVAAALSLSLVVTILWSKDAIAGLVINIAVLIVLGLIALGSHLQQQNRG
                     LRQAKPV"
     gene            complement(8358..9440)
                     /locus_tag="VGR29_01000"
     CDS             complement(8358..9440)
                     /locus_tag="VGR29_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072191.1"
                     /translation="MISHPSNPAIDRSTVHHGTKIDDRSRMITISASSAVVWLSGLII
                     ILSLVAASAGLFWQDGEGPTTVTTVHGETVELQGEGVYRYDSILKAGANKGADVVTLV
                     VSIPLLLVATYFFRHGSLRGAWLLTGTLVWFLYLYASLALGTAYNNLFLLYVALFSAA
                     LGAFVLAFGSIDLQALPAQVSSRLPRRGLAFFMIGSGVVTLGVWIEAPISALIKRQPP
                     SVIEHSTTLFTHALDLAIIVPAAILAGVLILRRAPLGYLMAFSLLILEVLLAPMIALQ
                     TAFQLQAGVTFTTAEIVGPIGGFVVIALIAIWFLVALLRTIGVDESALQQSGATSYQC
                     CEQGSDRDALRADGLVHQTRARLEEW"
     gene            complement(9656..10159)
                     /locus_tag="VGR29_01005"
     CDS             complement(9656..10159)
                     /locus_tag="VGR29_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072192.1"
                     /translation="MRQVIVDIRFLILKESWIMHAYDASKRPAVHATHTSYRTTRRDR
                     PIKLLAVELFMGFLALTCGGLLLVDGLGMPESTLESSPFDSFLIPGLLLSGVVGGSML
                     LAARLIWFRRPMAPIMSLLAGIILLCWIIVEAIMVHDGRGLQVAVAIYALVTIAMASG
                     FLRRNPR"
     gene            complement(10323..11168)
                     /locus_tag="VGR29_01010"
     CDS             complement(10323..11168)
                     /locus_tag="VGR29_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072193.1"
                     /translation="MTTMTRSKGYILTAIAAGLYIFLGIAFADGLFNLDNFWQSEEIF
                     SSSWVSYLLLALIIAGGVMQANRIPEAGVEIDAGTSTGNGQIEDPKGWRLLLGNTYLA
                     VFWMPLRFFVGQEWLAAGEHKIRDDAWMSGGSALKGYWERAVTIPEAPARPAITYNWF
                     RDFLQYMLDHEWYTWFAKLVAIGEVLVGVGLIVGGLVGIAAFFGTVLNFNFLMAGTVS
                     SNPVLFGLTVFLVLGWKVAGYIGLDRVLLPALGTPWQVGKLTSMWHRDGTGQGPINHQ
                     HPRTA"
     gene            complement(11910..12398)
                     /locus_tag="VGR29_01015"
     CDS             complement(11910..12398)
                     /locus_tag="VGR29_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2072194.1"
                     /translation="MYQRLVIPLDGSEIAERALIEATELASLSHTPMHLVRVIDSASA
                     DMAVVSGKLTDSEDTAVLLADDVDDACQYLDDVARRLGDRGHRITHEVRCGPAAGQLI
                     DAAQPGDLYVMASHGRTGLSRWFMGSVAEEVVRRIPVPVLLVRAGTPVKPSDTRRSLA
                     WS"
     gene            complement(12473..13333)
                     /locus_tag="VGR29_01020"
     CDS             complement(12473..13333)
                     /locus_tag="VGR29_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072195.1"
                     /translation="MMLITRAKMYGLTAIAAALYLGLGIAFGNGWGDEGIFSFGKFWE
                     GGNADIGYAGSSWLSYVLLAAIIAAGVMLANRIPEDGVQISAGDTSGEGQVHDPRGWR
                     LLLGNTYLSVLWLPIRFFVGQEWLAAGEHKLRDSGWMDGGTSLQGYWERAVAIPEAPA
                     RPAITYDWYRDFITYMLNHEWYTWFAKVVAIGEFLVGLGLLVGALVGIAAFFGTVLNF
                     SFMMAGTVSSNPVLFGLAVFLVLGWKVAGWIGLDRFLLPALGTPWEPGKVTHLHPYRG
                     NAATPTAQTH"
     gene            complement(13642..15594)
                     /locus_tag="VGR29_01025"
     CDS             complement(13642..15594)
                     /locus_tag="VGR29_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072196.1"
                     /translation="MTYRASRRQFIVGVLGISAAPATLPHGASAQIATPSNSNTHSAP
                     EFVVSIGSRGENDLAFERAEFGIVGIYDGDWFEQPGFPHLLDNLAASPQAFHGVRYFG
                     AFTAGQPEQFLPKTGGSVWTRADAPIDFSVTFDALEALTSRGLIPFISLGFFPPASSG
                     SPVQPPREWDAYKRLVRTFFEELAADPRFGREAIAGWWFEAWNEPNEGRFWTGSIDEF
                     FALYRATSEAIAETGLSVRFGGPAIAYKPQASPRDGAPWMERFLRFVASEPELQCDFI
                     SLHRKGTVGNDPPDPRRMYEAAVTTADQMLEIDPERFAGMVIINNEADEKVGFEHPYA
                     PRLDEHNAAWLATVTTIHGGLAARYEDAGFRFIGAADNANLQLVEEPFDGRRSIMTQA
                     RDSETDLLKLPAYAFYELLSLLGDCIGTVVAGADQVVPRTDIYHLATFAERHAGCLIT
                     HYPDPGNSEQSPHTLEYVVTDIPWGRVNIARFQIDNELSNAHTAAGGSEDNPFPVPDP
                     EGLGVIRQAQELAVARPIERDVSLDDGTYREPLEIAPYTTICLWITPFEATTPASPEW
                     LSIEVQDGNVVLRWTPDGETNFYSYEVFLMTNDEPGPRLTPEPLRSALWVDTAPPHGS
                     RSYGVRTVTASGMTSPIVASDAVTVR"
BASE COUNT         3109 a         4781 c         4998 g         3084 t
ORIGIN      
        1 cagttcgacc tgcccccctt cctcggcctg ggatttggct tcggtgtcgt gggggtcctg
       61 ttcagcttga tgggacagcg ggcccaacac cctcacgggg aagtggaggg gggaacggcc
      121 cccctgacca ggatggagca cgatatccag gaagtgctca gggaggtgga ttattcctcc
      181 ctgatcttct ttgtcggtat cctgctcagc gtggcggcat tggactcatt gggcgtcctg
      241 gcagaagtgt cccagaccct gtttggggct gttcccagtt tttcccgggt cgcagccggg
      301 agcatactga tgggatgggg gtcggcgatc gttgacaatg tgcccctgac cgccgtggcc
      361 atcgatacga tccccacatc ggattccagc ctctggtccc tgctggccta caccgttggc
      421 acggggggat cacacctcat catcggaagt gccgccggcg tcgtggtgat gggaagggtg
      481 aatgcgctca cgtcgcttgc ctacatgcga attgctgcat tgcccgtact ggttgcctac
      541 ctgtccggcg tcgtgatctg ggtggctcag atcctggtct tcggctagag gcggaatggg
      601 ggcacgagtc ctcctgatcc ggcaggcgtt tacgctgcat ctgcgagcag gaatccatca
      661 atacgcaagg actggtgcga ctcaaaacct gtccatgcca gtgggttgca ggaaccgagg
      721 agaactgtca cccgtctccc ctcagagacg ggtgacagtc aggaagcacc gcatttcagg
      781 cgtcaatgac gctcaggttg ccggatagaa ggccgggttg aagcgccggg cgcaaaagcc
      841 tgttgcccgc gacaggtcga ggtcgtgcac cagcaattgc tcctgaccat agggaacatg
      901 ggccacgcac accccatctg gatcgatgag gctggtcgcg gaatcctggt agcgcatggc
      961 gttgttgacg ctggcgaagt agatggtatt ctccacgctg cgcgccacca tcgccttctc
     1021 gtagtaggga gaagccgggt ctccccagcg atcgatggtc acaccggtgc ggtcgctgcc
     1081 ggtgacctga gggtgaaaga cgacgcgcgc gccgcgtacc gccgaccagc ggacggcctc
     1141 gggatagcgc caaccctcat ggcagatggt gatgccgaac gggacaccgt cgacctcgaa
     1201 cagtcgccgc cgcccatcgg gtacgtagaa cggctcttct tccggtggga tctggttctt
     1261 cgcctggtag ccttcgatga caccatcacg cgagacgacg aaggcgacgt tgtgcagccc
     1321 cagggtggtt tcccactcca tcccgataac gactgctacg ccgtgccgct gggctgaggc
     1381 acggatctct tcgagcgccg catcctgacg gacctgatcg tggggcggga cctcaacgtc
     1441 ctggccccgc aggccgggga tataggcctc cgggaaacag acgatggcca cctcgcgcgc
     1501 ggcagcctcc cccaggaatc ggtccacgat ttggagacgc tcttccaccg acggcgcgtt
     1561 ccgcgcggcc gccagaccga tacgtaagtt cccgttggga tttgtccgtt ccattggccc
     1621 gcacgcctcc agatccgcag ccttgatcgc atgtacgatc tcgattgcgc atcgtagcac
     1681 gaggaggtga aggagtacgt gaaggagatc ggctcgccgg tgctttcggt ccgaagcagg
     1741 attggcctcg ggaacgtgcc ggggcatcga gttaatcacg ggaatgggta tggggttcct
     1801 gacgaccgcg ggggatggaa aatggagacc ttgggccaat ccttggagcg gcccagggtc
     1861 aacttgtcag tccatgcgta acaggaacga ccggtagtac tacggattac gatgcgccag
     1921 tatcctcggt cgatggcggt tcttccgagc gcggtgggca gaagccctgc atcgagcagc
     1981 cgctgggaca ccaaccgggt ccggcgcatc gtaacgttct ccctccctat tcctccacaa
     2041 tcagggcagt caccatgccg aacattccat gctcggattc ggcgtgactc aggatgtggc
     2101 aatggaaggc ccaggtgccc ggctccgttg catcgatgat ggcctcaaag cgttgaccag
     2161 gggcgatgtt gagcgcgtcg cacaggtagg gctggggcaa gaggtaccca tccgcggcgg
     2221 tgacggtcat cggcatcccg tgcaggtgca tggggtgaat catcaagcct tcgttcatgt
     2281 accggaccag gatcttttcg cccttcttgg ccatgagcgg ctgggtcgcg gggaagctct
     2341 tgccattgag gctgaagccg ccgattggtc cgtcattcag gacgatggtg tactcccggt
     2401 caaaggcggg ccggctggac ggatcctttg gctcgacgat gaacgatccc aggagaccct
     2461 gggtgacctg gaatgccgag ttatggtgcg agtggtacat gtgggtgcca gcattgccgt
     2521 cacggatctg gaactcatag gtgtaggttt cacccggctt gatgggaggc tgggtgatga
     2581 acggcacgcc gtcctggttg ttgggcacga ccagcccatg ccagtgaacc gccgtgctct
     2641 gcggcagttc gttggtcacc tcgaatcgga ccttgtcccc ctcggtgacc cggatctccg
     2701 gaccgggaac tacgccgttg tacgtccagg catcgacaaa ctcgcccggg gcgaactccc
     2761 agttcgtcac ctggcaggtg aggctgaaga ccttgacgtc gccatccatc tcatactcaa
     2821 gcggctggcc gccgagtccc tcagtcttcg ccggaaagga cttgatgccc gcctcatgca
     2881 tggcatccat ctcgtcggcg gttggctcat ccttcttttt gctggtggtc tgggctgacg
     2941 ccaccttctc atggtcgtga tcggtcgatt gcgccagagc tgccggttcc cgttcgatac
     3001 ggacattgac tggtgacagt gcggtgccga tcgctccagc ccctgtcagg cctgcaatgc
     3061 ccatgaatcg tctccgggtc actgcctgtg gtgatgtctg ccgtgccatg ttgcctggtc
     3121 ctctcctgat agatgcgtcg gtttgttcct gctatgcaat gggcaggaga tgccccgcgc
     3181 gctcgacctt tcggtcggta cgaagctctc ttgtcatggc cgtagtgtgt cattgagggc
     3241 attgcaaacc ggacaaaaat gcgagagccg gtgttacaaa cccgtgtcaa ttccatggcc
     3301 caaaacctgg catgactttg taaccgcggt gaccagttcg ttacctgtct gcaacggcga
     3361 tcccgctaac ctgacgtcga gggctggatt cggctccccg gtgagacgcc ctttcatcag
     3421 cgtcttatcc cggtcaggtc atccgaaagg agttgggtga tgattcggtc ccgccttgcg
     3481 ggcatttgct cgattgcgct tggtgccggg attgccgcga gcgcaatcct tggcccgctt
     3541 gtcctcaagg ccattacgtt ccgcacgtcc gccaacatcg agaatcagtt cgttggcggt
     3601 gaggtcgtct ccctcgcagt tgttgccccg atggctgtcg ccgccggggt gctttggctg
     3661 cgtggccatc gcctggctcc cccactcgcc ctgggccccg ccctctacgc tgtctacacc
     3721 tatacgacgg ccgttattgg gcaggaatat ggccgctatg acggcaacgt ggagaagttc
     3781 ttcccgctct atgccgggct ggtcgctggc ggggccgcca ttgccgcctt tgcctgggga
     3841 cagctgggcc aaacggggac gtccctcccc ccaatcgcct gcgacggacg atggcgggca
     3901 tctttctcgg gcttgggagc tttttcgcgc ttgcctgggc gcagcagatc cggcttgtct
     3961 acatcggcag cgcgacggcc gagtatcagg aagggccgac catcttctgg ctgatcaagc
     4021 tgctcgactt cggcttcatg atccccttgc tgctggcgac cggcgtgggg ctcattcgcc
     4081 ggaaccggac ggccatcaag gctgcgtacg gtctggcgac attcgcgaca tgcctcgtgg
     4141 gctccatcat aggcatggcc gtggtgatgc aggcgaaggg agatccatca gcccagccgg
     4201 tgatgctggt cgtcctcata ccggccacga tcggtctggc tctggtgacg gaacatctcc
     4261 ttcgctcgta cgtgcagggc acccgggaac ggccggagtt ctccgcgact cctcgggtcg
     4321 gatgggagat ggggtagacc cggcatcgtg agtggcaatc ggccaggtct gtcgaggtat
     4381 cagatggaga cttcgacaga cctgcaatag atattcctga aactgaatgc tgagtgaggg
     4441 ggtagggcgt gtggtggggc catcgccttc gcgcattggg ctccatggat gctccctaga
     4501 tgcgctggca gtcgcctccc gctgtgccct attcctgtcg cgccggggaa cctcctggtg
     4561 gttccagccg gtaccccaca ccagggaagg tcgagatgat cgattcgtcg cctgcatcgg
     4621 gttcgagctt tgctcgcagc cggctcaccc aggttcgcag gtaatgcacc tcatcgcgga
     4681 actcaggccc ccagatgcgg gtgagcagct cggcatgaag catgatccgg ccgggattcc
     4741 ccgccaactg gctcaggagc gcccattcgg tccggctgag acgaatctcc tcgcccctga
     4801 cgagaacccg tcgctggtca agatcgatct ccaggtcggg ataacgcagc atctggttcg
     4861 tggtggctgg tgaatcgcca ccgctgcggc gaaggacagc agtgatccgg gcaaggagct
     4921 catccggatt gaagggtttg gtgacgtaat cgtccgcccc gctctggaaa ccctgtaact
     4981 tgtcgatatc gcccgtgcga gcggtcagca tgatgaccgg cactcgtgga acctggctct
     5041 ggctgcggat ccggcgcagg gtttcgaacc cgtccatctc cggcatcatc acgtcgagca
     5101 cgacgaggtc cggccgttcg gtattgatga tgttgagtgc cgtcgggccc cgatcggcgg
     5161 tcgctacccc gaacccgtcg gcttccaggg tagcccgaag cagtcgcacg atcgacgctt
     5221 cgtcatcaac cactagcacg agctgcttgc gcacgctggt tcctccaaat cgatgtgaat
     5281 ggccaatgag tatcccgccc tgatgctcga catggagcga agagcggcga tcaggtgtct
     5341 gaccagtcgg atgagaccgg caaggtcacg gaaaaggttg cacccttgcc cgggccgggt
     5401 gaggagaccc tgatctgccc gccctgggcc tcgatcaggt ggcggctcag gtagagcccc
     5461 agtcccatgc cccgcacggt agggtcggcg cccggccggc ggaaccgctc gaagaccgtg
     5521 cccaggtgct cggtggcgat gccggcgccc tcatccgcga catgaatcgt tatgctgccg
     5581 ttatcacagg tgacatacgt ggtgatcgtg ctcccgggcg gtgagtattt cgccgcgttc
     5641 tcatagaggt tgcgaagcac ctggttgagg agctcggcat ccccctccac cggtggaacg
     5701 ctgttggaga tatcgacaac aaaccggtga tcgcgtgatt ttgcctcggc ggtcgcggag
     5761 gctccacgta ccaacggggg cacgaggagc ggttctgttc cgggttccag ccgtcccgcc
     5821 tggatcgccg tgagggtaag catgttggcg agcatctgct cgagcctggt gctttcggtg
     5881 acgatatcgg tgagcagcgc ttgctgcgtt tcgcggtcca gcgtctcccc gcgttgcgcc
     5941 agcaggtggg cgccgccgtg gattgcggtg agaggcgtgc ggaattcgtg tgagatcaac
     6001 gacaggaagt cgtccttgag ctgttccgcc tcccggagcc gggtgacgtc ctgaaacaca
     6061 acgaccgccc ggacgacctc gccaaggcga ttacgcaatg gcaccgcctg gacgagcacc
     6121 ggcaccgtac gatcatcgcc gatgcggagc gtgagctgac gattccgtac gacctcgccg
     6181 cgaagggcac gcatggcggg ccattggatc cggggcaacg ggctgccgtc cgcgcgcagg
     6241 aagtgataat gccgtccggg ggtatcggag ccggggcgtg ggctcaccat ctcgcgggcg
     6301 gtggcgttgt cgagctcgat ctcgccttcg ggggcggtct ggatgatgac gccggaggga
     6361 aggctgtcga ggatgacgcg tagctgatcc cgctgctcct cggctcgccg ccggctgtcc
     6421 ccgacttccg agaagagctg ggcgcgatcg attgcggttg cggcgtgcgc agcaaggatc
     6481 tggatcgcct gcaggtcgga ttcgtcgtag ttccggtttt ctaggcgctc ggtgagatag
     6541 aggttcccca ccacacgatc tccgatcagg agaggcacac ccagcagcga atgcattggc
     6601 gggtgatggg gtgggaagcc cacggatcgg ggatcctcgc caatattggg cacgatcatt
     6661 ggcgagcggt cgcggataag ggcgccgagc ataccgtgcc cctgtgggat tggcccgatc
     6721 cgcgcccgtt cgagggagct gatgccggac gtgatgaact cggtgatgtg gccgtgctcg
     6781 tcggagacac caagggcagc gtatttggag ggaatgacgt cacgcgcaag atcgaccaac
     6841 cgctgtagca ctaccgacag ctggacctca gatgcgacat cgagagcagc gatataggcg
     6901 gcgacgacag caagattggg cggggaggat ttgctggatt catccgacgg cgtttgcccg
     6961 ttgaccgatc cataaccttg cgccactacc agaccccccg ctcgtcggac tgaaccactg
     7021 gttaccagga tcgggtcgct gtcatggccg tttcaccggt cattgcgccg gctagcgttc
     7081 gtcccgatcc cttcccgctt ccagtatagg atgaggcctt gaccagcaac acgggtatgg
     7141 acacgcggcg gacgagatcc tcggcaatgc ttcccatgaa ccagcggctg atcccgctcc
     7201 ggccatggga cgccatgaca aggagatcac ccggtttcga gacggcgatc agctgatcca
     7261 caacctgtcc gtgccggagc tcgtggctca cctcgtatcc ccgggctgca atccgttggg
     7321 cagcagatcc caggtactgc cggaccgagt cgatctcgtc ggcgagcgac gcagagagtg
     7381 ctagagggtt gagcatcgcg gtgtacgggg cagccgtgtg gtcagcagtc ccatcgatca
     7441 cgcggacaag atgaattggc gcgtgggtca gggtggccag cgtttcagct tcaacgaggg
     7501 cacgctcggc caggtcggag ccatcgaggg gtacaacaat tcgctggtac ataagagcct
     7561 cccatgagcg tccctcagag ctgtgctcgc agtgacacat cgacaagcga gcgagttgcg
     7621 ctatggcaag actaggggaa tgtatgtctc agaaccgatc acgattctcc ctgaggcgtt
     7681 acaagaacgt gtcaatattg ctcaagcatc gaattcggag tgaaggttgg tatggcgcag
     7741 gtcgcattgg gccgacgact gcgcaagtgc cttcacaggg cggatatcta ccattgacaa
     7801 gatccatgta acgagaccgc caaacagtgc gtccgccggt ctctcatcta ttgaacgacc
     7861 gatgttgaag ccgttagatg cttcttcctg gtcgctcggc tcatgatggc aggttgcgct
     7921 cgcatccttg ccctacacag gtttcgcttg acgcagccct cggttctgct gctggagatg
     7981 cgacccaagc gcgatcaggc cgagcacaat gagcacggcg atgttgatga caagtccggc
     8041 gatcgcgtcc ttcgaccaga ggatcgtcac cacaagcgag agggaaagcg cagctgccac
     8101 tgctggtggc caccacggtg cccgacgcag taccccgatt ccagccccga tgaatgtcag
     8161 ccccgcgagc aggaagagga cgccaagcgg agagacaact ccgtcatccg catgctccaa
     8221 caggatgttg gggtggtgag aaatatcttc aaaggttgtc atgtttcacg agccgagcac
     8281 tcccgggccg tgtgccacac catgcaggat cagcagcaca ccgaataaca ggcttgcggc
     8341 cttctgaagg tgtttcatca ccattcctcc aatcgtgcac gggtctgatg tacaagtcca
     8401 tcagcacgaa gcgcatcgcg gtcactgcct tgctcacaac attggtagct ggttgcccca
     8461 ctctgttgca gcgctgattc gtctacgccg atggtgcgca ggagggcgac aaggaaccag
     8521 atcgcaatca gcgcgatgac gacgaatccg ccaatggggc ctacgatttc agcagtggtg
     8581 aatgtcacgc ctgcctggag ctgaaacgct gtctgcaacg caatcatcgg cgccagcagg
     8641 acctcaagga tgagtaacga gaacgccatc aggtaaccca atggagcacg acgcagaatc
     8701 aggacaccag ccaggatcgc ggccgggaca atgatcgcca gatccagggc gtgcgtgaag
     8761 agcgtcgtgg agtgctcgat cacgctgggc ggctggcgct tgataagtgc gctgatcggt
     8821 gcctcgatcc agacacccaa ggtgacaacg ccactcccga tcatgaagaa cgcaaggccg
     8881 cggcgtggca ggcgggacga gacttgggca ggcaatgcct gcaggtcaat ggacccgaac
     8941 gccagcacga aggcaccaag cgccgcggag aagagagcga cgtagagcag gaacagattg
     9001 ttgtacgccg ttcccagtgc caggctggca tagagataga ggaaccagac gagcgtgccg
     9061 gtgagcagcc aggctccccg gagcgaccca tgccggaaga aatacgtagc gacgagcagg
     9121 agggggatgc ttaccaccag ggtaaccaca tccgcgccct tgtttgcacc ggccttgagg
     9181 atggagtcgt agcggtacac cccctccccc tgcaattcca cggtttcgcc atgcaccgtg
     9241 gtgaccgtcg tcggaccttc tccatcttgc cagaacaggc cagcactggc ggccaccagg
     9301 gacagtatga tgatcaaccc cgagagccaa accacagcac ttgacgccga tatcgtgatc
     9361 atccgcgacc ggtcgtcaat cttcgttcca tgatgcaccg tgctccggtc gatcgcgggg
     9421 ttcgaggggt ggctgatcat gacagaaact cctgtaacct caaacatgca ttcgcgtcca
     9481 cgtcggtaaa tcgtggtctg cgtgtcgtat gtactgtatg cactctgctg tcacagaggc
     9541 atcaaacagg aatagacgtc ataacagaat cgttgcaatg caaatctgtg caaatctgtg
     9601 gccgaatctc cttccacaac gtgcaaggat ttttccgatc cgtcgtggtg ctgactcatc
     9661 tgggattacg gcggaggaag cccgaggcca tcgcgatggt gacgagagcg tagatggcga
     9721 ccgccacttg caggccgcga ccatcgtgga ccatgatcgc ttcaacgatg atccagcaga
     9781 gaaggatgat ccccgcaagc agcgacatga tcggcgccat gggacgccgg aaccagatca
     9841 ggcgagcggc aagcagcatg ctgccgccga caactccgct gagaagcaga cccggaatga
     9901 ggaagctgtc gaagggggag ctctcaagcg tcgactccgg cataccgagg ccatccacaa
     9961 ggagcaggcc accacaggta agtgccagga aacccatgaa caattcgacg gcaaggagct
    10021 ttatggggcg atcacgacgg gtagtccggt atgaagtgtg cgtcgcatgt accgcgggtc
    10081 gcttgctggc atcataagca tgcatgatcc aactctcttt caggatcagg aagcgaatat
    10141 cgacgatgac ttgcctcatc gtttccggtg gatggcaagg gccactgcct acgtgtccat
    10201 tgacgctggg ccgtggttcg gatgcatctg gcgcaacacc actggtgcat gcgatgtctt
    10261 cagaaaaagt gagaccggcg aactactgtt cgccggtctc cttgctttcg aatgatcgac
    10321 agtcacgctg tgcgcgggtg ctggtggttg atggggcctt gtcccgttcc gtcccggtgc
    10381 cacatcgagg tcagtttgcc cacctgccag ggggtgccga gggctggtag caggacccga
    10441 tcgagtccga tgtaaccagc gaccttccac ccgaggacca ggaagacggt gagtccgaac
    10501 aggacaggat tggagctgac ggtgcctgcc atcaggaagt tgaagttgag caccgtgccg
    10561 aagaaggcag cgataccgac gagtccaccg acgatgaggc caaccccgac cagcacttca
    10621 ccgatggcca ccagcttggc gaaccaggtg taccactcgt gatcgagcat gtactgcagg
    10681 aaatcgcgga accagttgta cgtgattgcc ggcctcgcgg gcgcctccgg gatggtgacc
    10741 gctcgctccc agtagccctt gagggctgac ccgccgctca tccaggcatc atcgcgaatc
    10801 ttgtgctcgc cggctgcaag ccattcctgg ccgacgaaga agcggagcgg catccagaac
    10861 actgcgagat aggtgttgcc aaggagcagc ctccacccct tcgggtcttc aatctggcca
    10921 ttaccagtcg aggtgccggc gtcgatctca acgcctgctt ccggaatccg gttcgcctgc
    10981 atcacgccac cggcaatgat gagggccagg agcaggtagc taacccagga cgagctgaag
    11041 atctcctcgc tctgccagaa gttgtccagg ttgaacaggc cgtcggcaaa ggcaataccg
    11101 agaaagatat ataacccagc ggcgatggcc gtgagaatgt accccttgct ccgtgtcatc
    11161 gtggtcatga tggtgatcct ttctgcgtct ccctgacgct gcttccatac ccttggtttt
    11221 catccagctc ccggtctgcc tggcttctgc accggtgctt tctgttggga tcatgatgcc
    11281 gtgctaggca tggagaagac gtcacagcaa gccggccgac gtatcaatct cgtgacaaaa
    11341 ctggtggaga ggacggtgcc atggtgctgt gcttatgcag aggtgccaag cgggcgacag
    11401 actgtcacca cggctgtctc acagtctctg tagagccgtt gtgaaggcca gcgtctctcg
    11461 gtaatgtctt ccacgtcacc gctgtgcgaa ccacgtacgc cgcaatccct agtgaccgct
    11521 gagagtagat ctggaaccat gccgggtcat ggagggtccg ctgtagacgt gcaatggcac
    11581 ggatacccat gccccggcca tcaacaacgc gctgggcggg cagcgcctca ataccgtctt
    11641 gtgggaggac tgcctcggtt tcggcatggt ggcgcgtcga cgtactattc ggggagtcgc
    11701 gaaggtgcca gggagctcac cttggaggat gccgagtagg aacaggggat cgcgatctat
    11761 catcacatgg aggggggaaa tgtcgccgat gaggtatcgc tggaagcctc ttcattggcg
    11821 ccgtcgttct ggacacggct cctgacgttt gtgggtgtct catggagata caccttcggt
    11881 gtgtgattgg ctgacgctct ccttgcacat cacgaccatg ccagagaccg gcgagtgtcc
    11941 gatggcttca cgggcgtacc ggccctgacg agcaacacgg gaacaggtat gcggcgcacc
    12001 acctcttcgg ccacactgcc catgaaccag cgactcagac cagttcgccc atgcgaggcc
    12061 atcacgtaga gatcacccgg ctgggcagca tctatcagtt ggcccgcagc tggaccgcac
    12121 cggacttcgt gcgtgatgcg gtgtccacgg tccccaagtc ggcgggcaac gtcgtcaagg
    12181 tattggcacg cgtcatccac gtcatctgca agcagaaccg ctgtgtcttc cgaatcagtc
    12241 agtttgccgg agacaacggc catgtctgca ctggcggagt caatcacgcg aaccaggtgc
    12301 atcggcgtat gggacaggct ggcaagttct gtggcctcga tcaacgcacg ctcagcaatc
    12361 tctgatccgt ccaggggaat gaccagtcgc tgatacatgg gttcctcctt tagaaagaaa
    12421 gagaccggca acatggtggt tgccggtctc tgtagccgtt ggccaacgag catcagtggg
    12481 tctgtgctgt tggtgtggcc gcgttgccac ggtaaggatg gagatgagtg accttgccgg
    12541 gctcccaggg agttcccaat gccgggagca ggaaccggtc aagcccgatc cagccagcca
    12601 ccttccaacc cagcaccagg aagaccgcca atccgaacag caccgggttg gagctcaccg
    12661 tgccggccat catgaagctg aagttgagca cggtgccgaa gaaggcggca atcccgacga
    12721 gggcaccaac cagcagcccc agtccgacca ggaactctcc gatcgcgacc accttggcga
    12781 accaggtgta ccactcgtgg ttgagcatgt aggtgatgaa atcacgatac caatcgtagg
    12841 tgatcgcggg ccgtgccggt gcctcaggga tcgcgaccgc tcgctcccag tacccctgca
    12901 gcgaggtgcc gccatccatc caacccgaat cgcgaagctt gtgctcgccg gctgcaagcc
    12961 attcctgacc cacgaagaag cggatgggca gccagaggac cgagagatag gtattgccca
    13021 gcagcaggcg ccagccacgg ggatcatgga cctggccttc gccactggtg tcgccagcgc
    13081 tgatctggac gccgtcttcg gggatccggt tcgcaagcat gaccccggcc gcgataatgg
    13141 cggccaggag gacgtagctc aaccatgacg acccggcgta gccgatgtcg gcgttgcccc
    13201 cttcccagaa cttgccaaaa ctgaagatgc cttcgtcacc ccagccgttg ccgaaggcga
    13261 tgcccagtcc aaggtagagg gcggcggcaa tggcggtgag accatacatt ttggcgcgtg
    13321 tgatgagcat catgaacgtt gacctttctg cgtctctcga acgcgttgtg catcccgagc
    13381 atccatccag cggccagtac tcctggcccg tggatcaggt gccctgtgtt gggatcatga
    13441 tgccgtgcag ggcatggaga agatgtcgca ggggcgcagg ggaccgtgtc aatctcgtga
    13501 caaaactggt ggaggccctg agcgagaaag cggccccttc cgggaggcgc ggccaagctt
    13561 ggccatgcaa ccgtgtaaac agggttcttt gggatcaata tcagtcccgg cgccccgcgg
    13621 tcgtcagaga acgaccgcgc cctacctcac ggtaacggca tcgctggcga caatcgggct
    13681 ggtcattcca gacgccgtga cggtgcgaac gccgtagctg cgagaaccat gtggcggggc
    13741 cgtgtccacc catagcgcgg accgcagcgg ctccggcgtc aggcgggggc cgggctcgtc
    13801 attggtcatt aggaagacct cgtagctgta gaagttcgtc tccccgtccg gcgtccagcg
    13861 cagcaccacg ttgccgtcct gcacctcgat ggaaagccac tccggcgacg cgggagttgt
    13921 tgcctcgaat ggagtgatcc agaggcagat cgtcgtatat ggcgcaatct ccaggggctc
    13981 ccggtacgtc ccatcgtcga gggagacgtc ccgctcgatc gggcgcgcaa ccgccagctc
    14041 ctgcgcctgc cggatcaccc ccagtccctc gggatcagga acggggaacg gattgtcctc
    14101 ggagccgccc gcggccgtgt gggcgttcga tagctcattg tcgatctgga accgcgcgat
    14161 gtttacccgg ccccagggaa tgtccgtcac aacgtactcg agggtgtggg gcgactgctc
    14221 actgttcccg ggatcggggt agtgcgtgat caggcagccg gcatgtctct ccgcaaaggt
    14281 cgcgagatga tagatatccg tccgcgggac cacttgatct gcacccgcta caactgtccc
    14341 aatacaatcg ccgagcagcg agagcagctc gtaaaaggcg tatgcaggga gcttcaagag
    14401 atcagtttcc gaatcacgcg cctgggtcat gatcgagcgc cgcccgtcga atggctcctc
    14461 aaccagttgc aggttggcgt tgtccgccgc gccgatgaag cggaatcctg catcttcata
    14521 tcgtgcagcg agcccgccgt gaatcgtggt aacagttgcc aaccaggcgg cgttgtgctc
    14581 atccaggcgc ggtgcatagg gatgctcgaa accaaccttc tcgtccgctt cgttattgat
    14641 gatgaccata ccggcaaagc gctcgggatc gatttcgagc atctggtccg ccgtggtgac
    14701 tgccgcctcg tacatccggc gtggatccgg gggatcattc ccaaccgtgc ccttgcgatg
    14761 cagggagatg aaatcacact gtagctccgg ctcgctggcg acgaacctga ggaatcgctc
    14821 catccacggc gcgccatccc gtgggctggc ctgcggcttg taggcgatcg ctgggccacc
    14881 aaaccgcacc gacaggccgg tctccgcaat cgcctcggac gtcgcccggt agagagcgaa
    14941 aaactcatct atcgagcccg tccagaaccg gccctcattg ggttcgttcc aggcttcgaa
    15001 ccaccagccg gcaattgctt cccgcccgaa ccgtggatcc gcggccagtt cctcgaagaa
    15061 cgtgcggacc aacctcttgt acgcgtccca ttcgcgaggg ggctgcaccg gcgatccgga
    15121 gctggcaggt ggaaagaatc cgagggaaat gaatggaatc agtccccgcg aggtgagcgc
    15181 ctccagcgca tcgaaggtca cggagaaatc gatcggtgca tcggcccgcg tccagacgct
    15241 gcctcctgtc ttggggagaa actgctcggg ctggccggcg gtgaaggcac caaagtaccg
    15301 gacaccatga aacgcctgtg gagacgccgc cagattgtcc agcaagtgcg ggaagcccgg
    15361 ttgctcgaac cagtcgccat cgtagatgcc aacgatgcca aattccgcac gctcaaatgc
    15421 aagatcgttc tcaccgcgtg acccgatcga tacaacgaac tcaggggcgg aatgtgtatt
    15481 ggaattggag ggggtagcga tctgggccga tgccccatgt ggcagggtgg ccggggccgc
    15541 actgatcccc agaacgccca cgatgaactg gcgccgggat gctcggtacg tcatgtcgtg
    15601 ctcatgtcca ttgatgaaaa accggattat ccggttccac ttagcctcgc cggtagcctg
    15661 tcatgctcgc cgaatatggc tttcgcgtga gcatggatag tgatctcgtc gctgccgcct
    15721 gcaaagggcc gattccctag cttaacgatg taccatgatg tcgggtctct accgtcgaat
    15781 atcgtcgtca tcccatacaa gggagcagca ctatgtccac gcaccatcca cgaaccagtt
    15841 tcacccacga ggagaatatc gaccatgcca agggcaacgg agcggtgctg gccatggcgc
    15901 tggttgcccg tgcacgtgag cagggagaag caccggagac ggtagctcgc tggctcggga
    15961 ggatccttgc tc
//
LOCUS       DASYWM010000026         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_639048, whole genome shotgun sequence.
ACCESSION   DASYWM010000026 DASYWM010000000
VERSION     DASYWM010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639048"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGR29_01030"
     CDS             complement(<1..188)
                     /locus_tag="VGR29_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072197.1"
                     /translation="MQHKVSRSHHVPEQIVRYQGDQLILQPVCRFDPAEAVGALQRCQ
                     RVLAIDIGGDKIRSAHYE"
     gene            complement(348..548)
                     /locus_tag="VGR29_01035"
     CDS             complement(348..548)
                     /locus_tag="VGR29_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072198.1"
                     /translation="MSDHHTRPRLLTGDRPTGPLHLGHYVGTLQNRVRLQHDYDCFFI
                     IADVHILAEEWTRSPPWLPAAP"
     gene            complement(615..1109)
                     /locus_tag="VGR29_01040"
     CDS             complement(615..1109)
                     /locus_tag="VGR29_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV2072199.1"
                     /translation="MYCQRCGHNTEERQIDGRMRPLCVACGAVTWLDPKLAVAVVIER
                     EGKILMALRAEGAREAGKWSIPAGFVDRGEVVENAAIREIAEEVGLDVALGPILAVIS
                     RPDEPVVLLVYPAMSAKGDPTPNDDVQDIGWFLPDALPELAFAHDREILSIWGDWRAS
                     HAAD"
     gene            1262..>2541
                     /locus_tag="VGR29_01045"
     CDS             1262..>2541
                     /locus_tag="VGR29_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEV2072200.1"
                     /translation="MTTKTDVLIIGSGQAGSPLASAFAAAGKRTVLVERKHIGGTCVN
                     VGCTPTKTMIASGRVAYLSRRATEYGVQTGDVTIDMQSVRERKRDVVERWRSGSEKTL
                     LGKENPRVIFGEARFIGPHRVVVTLNKGGEEVLEAATIVIDVGERPAQMGVPGAEDVP
                     ILDSTTVMELGDVPDHLIIVGGGYVGLEFGQLFRRLGAEVTIVHRGSHLLSREDADIG
                     EAVGKVFEDDGIAVMLDAEAASVSQHAGRITLVVSQDGERRQITGSHILAAAGRVSNA
                     DSLSLDAAGVQTDEKGYIPTNDTLETNMPGIYAVGDVRPGPKFTHISYDDYRVVKAHL
                     IGGQQRTVGWRMVPYTVFIDPQLGRIGMTEEQAREDGREFRVATMPMSRVARAVETAE
                     PRGMMKVIVDAANDMILGAAILGLEGGEIASMLQ"
BASE COUNT          504 a          710 c          859 g          468 t
ORIGIN      
        1 atctcgtagt gcgcgctgcg gatcttgtcg ccgccgatgt cgatggcgag cacccgctgg
       61 catcgctgga gcgcgccaac ggcctccgcg gggtcgaacc ggcacaccgg ctgcaggatg
      121 agctgatcgc cctgatagcg cacgatctgt tcgggaacgt ggtgagaccg ggacaccttg
      181 tgctgcatgt gttcccctcg gtgaagatcg acagaggctg catggtatcc gaacggaagg
      241 aggctggcaa ccgcggggcg cgcctggcag atagtggcac cacgctcgtg cttttgctcg
      301 gtagcaggaa catgcaggac tacggcagga ggttggcagc gtgtcggcta cggagcggcg
      361 ggaagccatg gaggactccg cgtccattcc tcggctagta tatgaacgtc agcgatgatg
      421 aagaagcagt cgtagtcatg ttggagccga acccggttct gcagcgtgcc gacatagtgc
      481 cctagatgga gcggtccggt cgggcgatcc cccgtaagga ggcgtggtcg cgtgtggtga
      541 tcggacatgc gggttccttt ccaggaggat gagcgatcgt aacaggacgc acctgccggg
      601 ctagtggtcg atggtcaatc agcggcgtga gaagcgcgcc agtctcccca gatagaaagg
      661 atctcgcggt cgtgggcgaa ggcgagctcg ggtaacgcat ccggaaggaa ccatccgata
      721 tcctgcacat cgtcgttcgg cgtgggatcc cctttggcag acatggctgg gtagacgagg
      781 agcacgaccg gttcatccgg acgggagatg acggcgagta tcggcccaag ggcgacatcg
      841 aggccaactt cctcggcaat ctcgcgaata gctgcgtttt cgaccacctc cccgcgatcc
      901 acgaagccgg cgggaatact ccatttgccg gcttcccgcg caccttcggc gcggagggcc
      961 atcaggattt tgccctcgcg ttcgatgaca acagcgaccg caagcttggg gtcgagccac
     1021 gtcactgcgc cgcatgcgac gcagaggggt cgcatgcggc cgtcaatctg gcgctcttcg
     1081 gtgttatgcc cacatcgctg gcagtacatg gtgatccttg atcagtccga tatgcgtctg
     1141 ttgcagtctg tcgtttctac cgtgacaggg ccgccgatcg caaggggtag acagtatgga
     1201 tggcattgtg cgaaaggtga cacatcacgt ccatgtgggt catcaaggag aagcgaacga
     1261 agtgacgaca aaaactgacg tgctcattat tgggtcgggc caggccggca gtccgctggc
     1321 aagcgcattt gctgcagcag ggaaaaggac agtcctggtc gagcgcaaac atatcggtgg
     1381 tacctgcgtc aatgtcggtt gcacgccgac caagacgatg atcgcaagcg gacgggtggc
     1441 atacctctcc cgacgcgcga cggaatacgg cgtgcaaacg ggcgacgtca cgattgacat
     1501 gcagagcgtg cgcgagcgca agcgcgatgt cgtcgaacga tggcggagtg ggagcgagaa
     1561 gacacttctc ggcaaggaga atccacgcgt tatcttcggc gaggcgcggt tcattggacc
     1621 gcaccgcgtg gtcgtcactc tgaataaggg gggcgaggag gtcctggagg cggcgacgat
     1681 tgtgatcgat gtcggcgagc gcccggcgca aatgggagtc ccaggagcag aggacgttcc
     1741 catcctggac tcgacaacgg tcatggagct tggcgacgtc ccggatcacc tgattattgt
     1801 cggaggcggc tatgttggtc tggagtttgg acagctcttc cggcgcctcg gcgcagaagt
     1861 caccatcgtc catcgaggct cgcacctgct ctcccgcgag gatgcggaca tcggcgaggc
     1921 agtagggaag gtgtttgagg acgacggcat tgccgtgatg ctcgatgccg aggccgccag
     1981 cgtctcccag catgctgggc ggatcacact cgtcgtgagc caggatgggg agcggagaca
     2041 aatcaccggc tctcacatcc ttgccgctgc cggtcgagtc tccaatgccg attccctgtc
     2101 cctggatgct gctggcgtcc agacggacga gaaagggtac atcccgacga atgacactct
     2161 tgagacgaac atgccgggca tctatgcggt gggcgatgtc cgccccggtc ccaagttcac
     2221 ccatatctcc tacgacgatt accgtgtcgt gaaggcacat ctgatcggcg gtcagcagcg
     2281 gacagttggc tggcgaatgg ttccatacac cgtcttcatc gatccgcagc ttggccggat
     2341 cgggatgacc gaggagcagg cccgggaaga tggccgggag ttccgtgtgg ccacaatgcc
     2401 gatgagccgt gtggcgcggg ccgtggagac tgccgagcca agaggcatga tgaaggtgat
     2461 cgtcgacgct gccaacgaca tgattctcgg tgcagcgatc ctggggctgg agggcggcga
     2521 gatcgcgtcc atgctccaga t
//
LOCUS       DASYWM010000027         9315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_545280, whole genome shotgun sequence.
ACCESSION   DASYWM010000027 DASYWM010000000
VERSION     DASYWM010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9315
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545280"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VGR29_01050"
     CDS             complement(<1..556)
                     /locus_tag="VGR29_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV2072201.1"
                     /translation="MPQASSTRKQDGRPLEWLTWPFSLLLPIALLLLLVSLAVAGNDP
                     RTVVLTVRDESGEPLPQAQVVVDDELFLTDANGQVRLEAVDANKDVTVHREGYVTMVG
                     TLEENTSSDQEVVLQAEKSSNAMDAAMAEKPFATTLIGQESGVQQQDSTPAATASPKG
                     ESNTASKQIAGTVTNGAGEPVGSAW"
     gene            complement(724..1566)
                     /locus_tag="VGR29_01055"
     CDS             complement(724..1566)
                     /locus_tag="VGR29_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEV2072202.1"
                     /translation="MMRINHVRQRLQQGEPSIGTWLSLPSPEAAEYISRLGFDWLVVD
                     TEHNAIDIRTLGQMFMGMAHSGVAPMVRIPWNSAENIKRVLDAGAWGFVVPMVNSREE
                     AELAVSAARYPPTGTRSVGGGRHAMSFDGSAKEYYGQANDQVLVVLQIEHIQGVNNAE
                     EILSVPGVDACFIGPNDLAASMGLGLGVPLESDQPDLVEAILHIRDTARKHNVSPGVH
                     TSGPEGVNARIEQGFQFCAMASELKFMLGGLSEGIDQLNWQPSDRTQRVEGSAAEEGT
                     IVRY"
     gene            complement(1812..3398)
                     /locus_tag="VGR29_01060"
     CDS             complement(1812..3398)
                     /locus_tag="VGR29_01060"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEV2072203.1"
                     /translation="MSSNATTNMAAADAAAHTMDDRIRQTLDERPLIVASNRGPVTFT
                     AEGNDRFSSRQGSGGLVTAIGAVLQEHQAIWIAAAMTKGDRERVKKAEREGHALITPE
                     DSDSNFKLRFVVPETDAYNKYYNEISNPLLWFLQHYLWDTPRAPDITHETWDAWRNGY
                     VKVNEMFADEILVACEDSQPGQEPIIMLQDYQLYLCGQMIRQKRPHATIQHFVHIPWP
                     DPDYWRLLPMEMRCSICEGLLGCDIVGFQTPNHVRSFMYTCEAYVPDCTIDYATRCIQ
                     WNDRVIQVRAYPISIDTDEVRKTAYSQEARAYERYLPTHWNEFTILRADRAEPSKNII
                     RGFLAFDRFLEVHPEFRDRVNFIAVTQPSRMDVEEYRNYLDDVSAIVGRINARYANVE
                     TGWQPIQLIMGEKYARVLAAMKWYDLLMVNSLMDGMALVAKEGALVNERSGVLLLSEG
                     AGAVEQLGEYALVVAPADVEGTANAIYQALSMPIAERQRRALALKRAIEREDVTVWFR
                     DQLSDIIRLERPDSEKCEDA"
     gene            complement(3633..3857)
                     /locus_tag="VGR29_01065"
     CDS             complement(3633..3857)
                     /locus_tag="VGR29_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV2072204.1"
                     /translation="MAQQASEQVTLQAPDISCGHCVSSVQNRLGSLEGIRSVTASAET
                     KLVNVEFDPTQISLDRIEAELDDEGYPVQK"
     gene            complement(3957..6686)
                     /locus_tag="VGR29_01070"
     CDS             complement(3957..6686)
                     /locus_tag="VGR29_01070"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV2072205.1"
                     /translation="MSQGRTSPIFQTSFPVEGMTCASCVRRVEKALQKVDGVSTVNVN
                     LATERASVDIEEGVSSDDLRAAVEKAGYTPGQIEIPAIAREPASADTSPDSGEVSFSV
                     EGMTCASCVRRVERALEKVEGVSAVNVSLATERATVAYGNGVTADDLRTSVEKAGYVP
                     GEISLAAPSPSPVSMPPDSGASQEERDAEERDRKRDAYITDLKRKSLISLAIGVVMMV
                     LMYVPLPMSERTLAPFLLIGATVVQFWAGKVFYQATWAALKHGATNMNTLVAVGTSVA
                     YGYSAFVTLWPHLAERWQIPYHLYYEASVIIIALILMGRWLEARAKKRTGDAIRALMG
                     LRAETARIIRDGREIDIPTDEVVVGDLVRVRPGEKIPVDGVLVEGSSSVDESMLTGES
                     MPVTKHTGDSIIGATINQTGSFVFRTTNVGRDTVLAQIVRLVEEAQGSKAPMQRLADQ
                     VAGVFVPAVLVIAALTFGGWLIWGPDPALSFAVITAVAILVIACPCALGLAAPTAIMV
                     GTGKAAENGILVRGGEALELARKIDTVVLDKTGTITQGKPSVTSIRMASDADENEMLT
                     LVAAAEAQSEHPLGQAMIALAEERGLALPPVSQFDSVTGKGIIATVDGRRLAIGNAGL
                     MDGHEIDHASLDAIVGDLARSGATPVMVAIDGEPAGVIGIADELKADSAETVKELEAL
                     GLDVWMITGDNAHTAEAIASQAGIRNVLAEVLPDEKAAKVRDLQADGRTVAMVGDGIN
                     DAPALATADLGIAIGTGTDVAMASSDITLIGGDPKTIVTAIAVSRRTVATIKQGLFWA
                     FAYNIALIPIAMGALFPAFGILLNPVLAAAAMAMSSVSVVTNALRLRDFKPPKDAAEI
                     LHPPLGARVRDAGYLVAIAIIALAIGAGALWLGETAGMGIEDTRDTMEMHHSD"
     gene            6931..7332
                     /locus_tag="VGR29_01075"
     CDS             6931..7332
                     /locus_tag="VGR29_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072206.1"
                     /translation="MVDRSRMQHPRRPGLSPRKDALQTPSEDRTMQENRRECPWCGST
                     NTLFYKRGFTGPTDERDQYFTCEDCGRWTYEIVSRTSRDMRVGQFRAGGSFRDHARKT
                     KYTISRVLKVGMNEVLLYLKPIVPRTDETDT"
     gene            7404..9029
                     /locus_tag="VGR29_01080"
     CDS             7404..9029
                     /locus_tag="VGR29_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV2072207.1"
                     /translation="MSDTIATAATTHTTSPLGQEVAKRRTFAIISHPDAGKTTLTEKL
                     LLYGGAVQLAGSVTARKSQRHAASDWMDIEKERGISITSTVLAFPYGGRQLNLLDTPG
                     HQDFSEDTYRTLTAVDSAVMVLDGARGIEPQTLKLFEVCRRRHTPIFTFINKVDRPAK
                     EPLDLMDEIEQTLGLQTYPMNWPIGDGQDFKGVYDRMTNTVQLYERTEHGSRRVPVQV
                     SDLSDPLLAEKIGEQQAAKLREEIELLELAGTPFDIDEVRAGRLTPVSFGSALTNFGV
                     QLFLETFIQFAPEPAPQRTDKETMEPEDDRFSGFVFKIQANMDPRHRDRVAYFRVVSG
                     KFERDMDAWHPRLKRKVRLARPMRLFGQDREVVDEAFAGDVVGLINPGTFTIGDTISS
                     EDVGQFTALPRFQPEHFALLRHSRADKYKQFLKGLTQIEEEGGIQLFYPVSSGSREPI
                     MGAVGALQFEVVRYRLEAEYNVETIMEPLTYSAARWVTGDDVEITSIGNGRGRMRAED
                     QEGRPVILFTTEWDLRYAEENAKNVTFSALAGI"
     gene            9065..>9315
                     /locus_tag="VGR29_01085"
     CDS             9065..>9315
                     /locus_tag="VGR29_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072208.1"
                     /translation="MAEATVQQALPHWNLDQYFPGLSSPEFASAVEEMKSDTASFSRL
                     VEEITSRESSSDVTADADSVLDALIRLLDRVSIIMPYVHL"
BASE COUNT         1813 a         2818 c         2770 g         1914 t
ORIGIN      
        1 cccaggcact ccccaccggt tcgcctgctc catttgtcac tgtgcccgcg atttgtttgg
       61 aagcggtgtt gctttctcct ttaggggaag ccgtcgcggc aggtgtgctg tcctgctgtt
      121 gaaccccaga ttcctgccca atgagggtgg tggcaaatgg cttctccgcc atggcggcgt
      181 ccatcgcgtt ggagctcttt tcggcttgaa ggacgacctc ctgatcggaa gaggtgttct
      241 cctccagtgt gcccaccatg gtgacgtagc cttcgcgatg gaccgtcacg tccttgttgg
      301 cgtcaactgc ctccagccgc acctgaccat tggcatccgt gagaaagagc tcgtcatcga
      361 cgacgacctg ggcctggggt aggggttcac cgctttcgtc tctcactgtc aggacgaccg
      421 ttcgggggtc gttccctgcg accgcgagag acacaagcaa gaggagcagg gcgattggga
      481 gaagcaggct aaatggccaa gtcagccact cgagcggtcg accgtcctgc tttcgggtcg
      541 atgaggcttg gggcatagaa tgaaacccgt cttcggtatg agacccatga gacggcgggt
      601 cacggcacat tcattgttca aatatgtctt ttggtgcacc aggagcacag ggaacgaacc
      661 ctgacctgcg atcgatggac caaggatgag cctaccacgc ccctgcgttc tggtgcgacc
      721 ggattagtac cggacgatgg tgccttcctc cgccgccgac ccctccactc tctgggtgcg
      781 atctgagggc tgccaattga gttgatcgat tccctcactg agcccgccca gcatgaactt
      841 cagctcgctc gccatggcgc agaattggaa tccctgctcg atacgggcat tgacgccctc
      901 gggacctgag gtgtgtacgc ccggcgacac gttatgcttt cgggccgtat cgcggatgtg
      961 gagaatcgcc tcgacgagat caggctggtc ggattccaac ggaacgccca gaccaagacc
     1021 catggaggcg gcgaggtcat tgggcccgat gaagcaggcg tcgacgccgg gaaccgagag
     1081 aatttcctcg gcattgttca cgccctggat gtgctcgatc tggagcacga cgaggacctg
     1141 atcgttcgcc tggccgtaat actcctttgc cgagccgtcg aagctcatcg cgtggcgtcc
     1201 tccaccgacg ctccgtgtgc ccgtgggtgg atagcgcgcc gccgataccg ccagttccgc
     1261 ttcctcgcga ctgttgacca taggtacgac gaatccccag gcacctgcat ccagcacccg
     1321 tttgatgttc tcggcactgt tccaggggat gcgaaccatc ggggcaacac cagagtgggc
     1381 catgcccatg aacatctgcc cgagcgtgcg gatatcgatg gcgttgtgct cggtgtccac
     1441 cacaagccag tcaaatccga gccggctaat gtattcagcg gcttcgggtg atgggagtga
     1501 aagccaggtg ccgatcgagg gttctccctg ctggagtcgc tgccggacgt gattgatacg
     1561 catcatgttc ctcctgacga acgagtggaa aaggacagac atcagtacgt acgtaatgta
     1621 atcgctctac tgagccggga atggagcctg acttcgtgaa gcctggcaca atgttggcaa
     1681 gcgtgacgta acccgtcaca atatgcacac gtttccttca gatatcgaaa tcgcgtagac
     1741 ggagcctgag gcggacaacc cgccggtgga taagcagagc cgtgcgcttt ttcgtccggc
     1801 tgtactaccc attaggcatc ctcacacttc tccgaatcgg gccgttcgag gcgaatgatg
     1861 tccgacaact ggtcgcggaa ccagaccgta acatcctcgc gttcaattgc ccgtttcagc
     1921 gccagggccc gccgctgccg ctccgcgatc ggcatgctga gcgcctgata gattgcattg
     1981 gcggttcctt ccacatccgc cggtgcgaca acgagtgcgt attccccaag ctgctccacg
     2041 gcgccggcac cctctgagag caggagtacg ccactacgct cattgaccag ggcgccttcc
     2101 ttggcgacga gtgccatccc atccatcagg gagttgacca tcaggaggtc gtaccatttc
     2161 atcgcggcaa ggacgcgggc gtacttttcg cccatgatca gctgaatcgg ttgccagcca
     2221 gtttccacat ttgcgtaccg ggcgttaatc ctgccaacga tcgcgctgac atcgtcgagg
     2281 tagttccgat actcctcaac gtccatacgt gatggctgcg tcaccgcaat gaagttcacg
     2341 cggtcacgaa actcgggatg gacttccagg aagcggtcga aggcgaggaa cccccgaatg
     2401 atgttcttgc tcggctccgc acggtcggcc ctaagaatcg tgaactcgtt ccagtgcgtc
     2461 ggcaggtatc gctcgtacgc ccgtgcttcc tgactatatg cggtcttgcg gacttcatcg
     2521 gtatcgatcg agattggata ggcgcgaacc tggatgacac gatcgttcca ttggatgcac
     2581 cgggtcgcat agtcgatggt gcaatcggga acatacgctt cacaggtata catgaagctg
     2641 cgcacgtggt tcggtgtctg gaagccaacg atatcgcagc ccagcaaacc ctcacagatc
     2701 gagcagcgca tctccatcgg cagcaagcgc caatagtccg gatcaggcca cgggatgtgc
     2761 acgaagtgct gaatcgtggc gtgcggtcgc ttctggcgga tcatctggcc gcaaagatag
     2821 agctggtagt cctggagcat gatgatcggc tcttgccccg gctgggaatc ctcgcatgcc
     2881 accagaattt catcggcgaa catctcgttc accttgacgt agccattacg ccacgcgtcc
     2941 caggtttcat gagtgatatc tggcgcccgc ggcgtatccc agaggtagtg ctgcaggaac
     3001 cagagcaggg gattactgat ttcgttgtaa tacttgttgt acgcgtccgt ttccgggacc
     3061 acgaaccgga gcttgaaatt gctgtcgctg tcctccgggg tgatcagtgc atggccctcg
     3121 cgctccgcct tcttgacgcg ttcccgatct cccttcgtca tggcggccgc aatccagatc
     3181 gcctggtgct cctgcagcac ggctccaatg gcggtcacga gaccgccgct tccctgccgg
     3241 gagctgaagc gatcgttgcc ctcggcggta aacgtcaccg ggccacggtt cgaggcgacg
     3301 atgagcggcc tttcatccag agtttgccgg atacggtcgt ccatggtgtg ggccgctgca
     3361 tctgccgcgg ccatgtttgt ggttgcattg ctcgacacgg tgattgcctc cttgcaggtc
     3421 ccttaatggt acgtacgatg agcaatgact ccgccaatgg gagcatcggc tcccttgtac
     3481 ggtcgatcgt cgagcgttcc caacctgcca tctcgcgtca gcatgaccgc aagcaccgcc
     3541 gagcgcaaga ggtttccctg cccttaacct gcacacccct tcgcatattg catgcgaagg
     3601 ggtgtgcaag tacgtgtttg ggagagtgac cgttacttct ggacggggta accctcgtcg
     3661 tcaagctccg cctcaatcct gtcgagggag atctgcgtcg ggtcgaactc tacattcacc
     3721 agcttcgtct ctgccgaggc ggtcacgctc cggataccct caaggctgcc gagtcgattc
     3781 tgcaccgagc ttacacagtg cccgcagctg atatcgggag cctggagtgt cacttgttcg
     3841 ctagcttgct gtgccatgaa aaatcttcct ctcgtgttgc cgggcgggtg tccgtcacgg
     3901 cattggccaa tgaaatcagt actgacgtag tacccatgtt tgggatggga gacaacctaa
     3961 tcgctgtggt gcatctccat ggtatcgcgc gtgtcctcaa tgcccatgcc ggctgtttca
     4021 ccaagccaga gcgccccggc gccaatagcg agggcaataa tcgcgatcgc tacaagatat
     4081 cctgcgtcac ggacacgcgc acccagtggc gggtgcagga tctccgcggc atccttcggt
     4141 ggcttgaagt cacgcagccg caaggcgttc gtcactacgc tgaccgagct cattgccatc
     4201 gcggcggcgg cgagaaccgg gttgagcaga atcccgaacg ccgggaagag ggctcccatt
     4261 gcgatcggga tgagtgcaat attgtatgcg aacgcccaga agagtccctg tttgatcgtc
     4321 gccacggttc ggcgagatac ggcgatcgcc gtcacaatcg ttttggggtc gccaccaatc
     4381 agcgtgatat cggacgacgc catcgcgacg tcggtgccag tgccgattgc gatgccgagg
     4441 tcggcggttg ccagtgctgg agcatcattg atgccgtccc cgaccatggc gacggtccgt
     4501 ccatctgcct gcaagtcgcg gaccttcgct gccttctcat cgggcaggac ctcggccagc
     4561 acgttccgga taccggcttg ggaggcaatc gcctctgccg tgtgggcgtt gtccccggta
     4621 atcatccaga cgtccaggcc gagcgcttcg agttccttta cggtctcggc ggagtcggcc
     4681 ttgagctcgt ctgcgatacc gatcacaccc gccggctctc catcgatggc caccatcact
     4741 ggcgttgcgc cggatcgggc gaggtccccc acgatagcat cgagtgacgc gtggtcgatc
     4801 tcgtggccat ccataaggcc ggcattgccg atggcaagac ggcgcccatc aacagtcgcg
     4861 atgatgccct tgccggtcac tgagtcgaac tgcgaaaccg gtggtaatgc gagtcctcgc
     4921 tcctcagcga gggcgatcat cgcctgtccg agcgggtgct cggactgcgc ctcggcagcg
     4981 gcgacgagcg taagcatctc gttctcatcg gcatcgcttg ccatccggat cgatgtcacg
     5041 gaaggcttgc cctgggtaat cgttcccgtc ttgtcgagca cgactgtatc gatcttccgt
     5101 gccagctcca gtgcttcccc gccacgcacg aggatgccat tctcggcggc cttgccagtg
     5161 ccgaccataa tggccgtcgg cgcagcgaga ccaagagcgc acgggcaagc gatcacgagg
     5221 attgcgaccg ccgtgatgac cgcgaaggag agggccgggt ccggtcccca aatcagccaa
     5281 ccaccgaacg tcaaggcggc gatgaccagg acggccggca caaagacgcc tgcgacctgg
     5341 tccgccagcc gctgcatcgg cgccttggag ccctgggcct cctccacgag gcgaacgatc
     5401 tgcgccagga cggtatcccg cccgacgttg gtcgtccgga agacgaacga tccggtctgg
     5461 ttgatggtgg caccaataat cgagtcgcct gtgtgcttcg tgacgggcat cgattcgccg
     5521 gtcagcatgc tctcgtcgac ggatgaggag ccctcaacga ggacgccgtc gacggggatt
     5581 ttctcgcctg gtcgaacacg taccagatcg ccaacgacaa cctcgtcggt cgggatgtca
     5641 atctcacgac catcgcggat tatcctcgcc gtttcagctc ggagccccat gagcgcccga
     5701 attgcgtctc cggtgcgctt ctttgcacgc gcttctagcc atcgccccat gaggatgagc
     5761 gcaatgatga tgaccgaagc ttcgtagtag aggtggtagg gaatctgcca tctctccgcg
     5821 agatggggcc agagggtgac gaaagcactg tagccgtagg cgacgctggt accgaccgcg
     5881 acaagggtgt tcatgttggt ggcgccgtgc ttgagtgcgg cccatgtcgc ctggtagaag
     5941 accttgcctg cccaaaactg gacgacggtc gcaccaatca gcaggaaggg cgcaagtgtc
     6001 cgctcactca tcggtaatgg cacgtacatt aggaccatca tcaccacgcc aatggcgagg
     6061 ctgatcaagc tcttgcgctt gaggtcggtg atgtaggcat cacgtttgcg gtcccgctct
     6121 tcggcgtcgc gctcttcctg gctggcgcca gaatccggtg gcatcgaaac cggcgatggg
     6181 gatggggccg caagtgaaat ctcacctgga acataccccg ccttctcgac ggaagtacgt
     6241 agatcgtcgg cggtaacacc attcccgtag gcgacggtcg cgcgctccgt cgcaaggctg
     6301 acattcacgg ccgagacgcc ttcaaccttc tccagcgctc gctccacgcg acggacgcac
     6361 gaggcgcatg tcatgccttc gacactgaag gaaacctctc cggagtccgg tgaagtgtcg
     6421 gctgaagccg gttcacgagc gatcgcagga atctcgattt gtcccggtgt atatccggcc
     6481 ttttcaacag cggcgcgaag atcgtccgac gaaacgcctt cctcgatatc cacgctcgct
     6541 cgctctgtcg cgaggttcac attgacggtc gaaaccccgt cgactttctg aagcgccttt
     6601 tcgacgcggc gtacacagga tgcgcatgtc atcccctcga cggggaatga tgtctggaat
     6661 atgggagatg tacgcccctg gctcatcgtg aggcccttcg tctgcaagcg ctaaacggaa
     6721 ctactatggg tttattgtac cccgaggggg tataatacgc aagcgacctt attgatctcg
     6781 ggccgcgttt ttgacgggga gccgtcccgg ctcgtgatgt accatagggt ctgtacttcg
     6841 gctgcggcgg gcgaggggtg ccggctcgta ttcacgcgtg accgctatgc tgcatctggc
     6901 gccgccgctc ccggacagga ggatggcccc ttggtggatc gatcacgcat gcaacatccg
     6961 cggcgaccgg gtctctcacc ccgcaaagac gcgctccaga cgccctcgga agaccgaacg
     7021 atgcaagaaa accgccgcga gtgcccctgg tgcgggagta ccaacacgct cttctacaag
     7081 cgggggttca ctggtccgac ggatgagcgc gatcagtact tcacctgcga ggattgcggc
     7141 cgctggacgt atgagattgt ctcgcgaacc agtcgcgata tgcgtgtcgg ccagttccga
     7201 gcgggtgggt cattccgtga tcatgctcgc aagactaagt acaccatcag ccgggtcctg
     7261 aaagtcggca tgaacgaggt tctgctctac ctcaagccga ttgttccccg cactgatgag
     7321 actgacacat agataccgcc ccaaatctcg cgtgcatgca cctcaagtcc cctcagcttt
     7381 accaaggaag tacgtactac tcaatgagcg acacaatagc tactgctgcc acaacacaca
     7441 cgacgtcacc tcttgggcag gaggttgcca agcgccgaac cttcgccatt atttcccatc
     7501 ccgatgccgg caagacgaca ctgacagaaa agctgctgct ttatggcggg gcggttcagc
     7561 tggctggctc agtgacggcc cgcaagtcgc agcggcatgc agcctccgac tggatggata
     7621 tcgagaagga gcgcggcatc tcgattacct cgacggtgct cgccttccca tatggtggac
     7681 gccagcttaa cctcctcgat actcccggtc accaggattt ctccgaggac acctatcgca
     7741 cgctgaccgc agttgatagt gctgtcatgg tgctggacgg cgcgcgtggc atcgagccgc
     7801 agaccctgaa gctgttcgag gtctgccgcc gtcgccatac tcccatcttc acgttcatca
     7861 ataaggttga ccggccggcg aaagagccac tcgacctgat ggacgagatc gagcagacgc
     7921 tcggattgca gacgtacccg atgaattggc cgattggtga tggccaggac ttcaagggtg
     7981 tttacgaccg gatgaccaac acggtgcaac tctatgagcg caccgagcac ggttccaggc
     8041 gcgtaccggt ccaggtgagt gatctcagcg accccttgct tgcagagaag ataggcgagc
     8101 agcaggcggc gaagctgcga gaggagatcg agttgctgga gctcgcgggc acgcccttcg
     8161 atatcgacga ggtgcgcgcg ggtcggttga cgccggtgtc gttcggttct gccctcacca
     8221 acttcggggt gcagctcttc ctcgagactt tcatccagtt tgcgcctgag ccggctccgc
     8281 aacggaccga caaggagacg atggagcccg aggatgaccg gttttccgga ttcgtcttca
     8341 agatccaggc aaacatggac ccccggcatc gcgaccgtgt ggcctatttc cgggtcgtat
     8401 ctggcaagtt cgagcgggac atggatgcct ggcatccgcg cctgaagcgg aaggtgcgtc
     8461 tcgcgagacc gatgcggctc ttcgggcagg atcgcgaggt cgtggacgag gcctttgccg
     8521 gggatgtcgt cgggctcatc aatcctggca cgttcaccat cggcgacaca atcagcagtg
     8581 aggatgttgg ccagttcacc gcgctgcctc ggttccagcc ggagcacttc gctttactac
     8641 gccattctcg ggcagacaaa tacaagcagt tccttaaagg actgacccag atcgaggaag
     8701 aggggggtat ccagctcttt tacccagtca gcagcggtag tcgtgaacct atcatggggg
     8761 ctgtcggcgc actgcagttc gaggtcgtac ggtatcggct tgaagccgaa tacaacgtcg
     8821 aaaccattat ggagccgctg acgtactcgg ctgcgcgctg ggtgacgggc gacgatgtcg
     8881 agatcacatc gatcggaaac ggccgcggtc gcatgcgagc agaggaccag gagggtcggc
     8941 cggttattct cttcaccacg gagtgggatc ttcgctacgc cgaggagaac gccaagaacg
     9001 tcaccttctc cgcccttgca ggtatctaac gaacctgaac ccgttttaca tcaagaggaa
     9061 acacgtggct gaagcaaccg tacaacaggc gttgccgcac tggaacctgg atcagtattt
     9121 cccgggactc agctctccgg agtttgcgtc tgctgtcgag gagatgaaga gcgacacggc
     9181 ttctttctca aggcttgtcg aggaaattac gagccgcgag tcatcgtcag atgtcacggc
     9241 ggatgcggac agcgtcctcg acgcgttgat ccggctactc gaccgggtct ccatcatcat
     9301 gccctatgtt cacct
//
LOCUS       DASYWM010000028        13109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_57218, whole genome shotgun sequence.
ACCESSION   DASYWM010000028 DASYWM010000000
VERSION     DASYWM010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13109
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57218"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VGR29_01090"
     CDS             complement(<1..323)
                     /locus_tag="VGR29_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072209.1"
                     /translation="MKNYTGTTAKPFGQRRTFSFLVSLFVIASFFSAVIAANGPTSRA
                     FAQAETSPADVVPAESVLYMDVELDQTTEQWTTFYELLDRAGLSGLAEQEANVSPEQA
                     GQLAA"
     gene            complement(514..648)
                     /locus_tag="VGR29_01095"
     CDS             complement(514..648)
                     /locus_tag="VGR29_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072210.1"
                     /translation="MELLEGLIRAVLTVGFVVLVILAIAALFMLVFTLATSFDKRVHR
                     "
     gene            complement(807..3416)
                     /gene="clpB"
                     /locus_tag="VGR29_01100"
     CDS             complement(807..3416)
                     /gene="clpB"
                     /locus_tag="VGR29_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEV2072211.1"
                     /translation="MAANQQYTEKAREAIVEGQQITEQRKLAQYEPEVLLYSLVSQAE
                     GIVPQILQRLSIDPKIIASELEKLIDAAPKLQFSSDAIISAGMRRVLQAAETEARQFG
                     DEYISAEHLLLGIMSVERSPAESLLRRYNIDRDRVLGILTDIRGNQRVTDPNPESTYQ
                     ALEKYGRDLTELARDGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAE
                     GLAQRIVRGDVPEGLENKRIISLDLGALVAGAKFRGEFEERLKAVLKEVTSSNGEILL
                     FIDELHTVVGAGAAEGSMDASNMLKPLLARGELHAIGATTLNEYRKYIEKDAALERRF
                     QPVMVGEPTVEDTISILRGLRERYEVHHKVKILDSALVAAATLSNRYITDRFLPDKAI
                     DLVDEAASKLRMEITSMPVELDETERRIMQLEIEREALRKEADVASKQRLASLEQELA
                     DLKEERDALRSRWDQERQAISAISELRDEMDRTRVEIEQAQRAYDYNRAAELQYGKLK
                     ELDTRLNELEQDLIRLQSGGKLLKEEVDAEDIAEVVSRWTGIPSTRLMEGEIQKLVQM
                     EDRLHARVVGQDEALEAVANAIRRARAGLQDPNRPLGSFIFLGPTGVGKTELARALAG
                     FMFDSDQAMVRIDMSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEAIRRRPYSVVLFDE
                     IEKAHPDVFNVLLQILDDGRVTDSQGRTVDFKNTVIIMTSNLGSQFIAGLGQGQDEEI
                     RHQVMDALHANFRPEFLNRIDETIIFRQLTKEQIGDIVQIQLEQVSKRLRDRDISLML
                     TPAAKDLIATLGYDPMFGARPLKRVIQKQILDPLALKLLNGEIREGETVTVNARNHEF
                     TFNGNLSNAQVVA"
     gene            complement(3519..3890)
                     /locus_tag="VGR29_01105"
     CDS             complement(3519..3890)
                     /locus_tag="VGR29_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV2072212.1"
                     /translation="MPQRFSEPLFVISVAARLVEMHPQTLRKYEREGLIAPSRTSGNL
                     RLYSDQDIEQLRQVKYLVEERGLNLAGVQLALDLTRELQALREASSRGKANGVSLEAE
                     HNRLLRMLGHVDQQELRKRRT"
     gene            3996..4688
                     /locus_tag="VGR29_01110"
     CDS             3996..4688
                     /locus_tag="VGR29_01110"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEV2072213.1"
                     /translation="MTPVYAHRGASAEYPENTLAAFRRALELHTEGIELDVHLSADGV
                     PVVIHDLNVDRTTDGSGLVSEMTFDELQSLDAGQGEKIPALGQVLDLVGDRPHVDIEV
                     KANEAGEAVLRELQGRNTRWLISSFDWDVLHYVRRQDADAVLWPLTIAATDEAINVAR
                     DLGAPALAILHKAIDQDIVEHLKEQGLDFWVWTVNDPEQARIYAEWGAIGICTDDPAA
                     IQQGLGERHPRT"
     gene            4802..5446
                     /locus_tag="VGR29_01115"
     CDS             4802..5446
                     /locus_tag="VGR29_01115"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV2072214.1"
                     /translation="MANAITIGRLVLLFVVIWLIYVGNVTIIAACMLLLMLVFAADGV
                     DGWVARKQGTTSTFGAVFDIAGDRMVENSLWVIFAHLDVIPVWVPLLVLSRGFIVDGL
                     RSLSFAEGMTAFGEKNMMRSPITQWLTAGRLMRALFGYMKAAGFVFLTGLVAWETKDT
                     NGTALGALYSQQWFRWFGWLLVWGAVALTVVRGLPVLWDAFPYMRELDRRKRAG"
     gene            5562..6662
                     /locus_tag="VGR29_01120"
     CDS             5562..6662
                     /locus_tag="VGR29_01120"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072215.1"
                     /translation="MAHDDKPKRRTAVFRKEDRHELRYQLSVAIAASLSWFFFLVPAP
                     IRNSFAERCGSLFFRVSHTYRDNVISNVMQVLGKTEEDAEVRATARSIFLNSALNFMD
                     LLTLTRRSGRYLMRSTHVVAGDWSTINNAMASGRGLILVTAHVGCFDFIGQSFLAHGL
                     KLTIVTGRTTSRFIFDGVTWLRSVRGGNFVEPTPSGVRAVIKALRRGECAVFVADRDF
                     FQNGRPATFFGRETTLPPGVIRIARDTGAVIVPIFTRRMRRGHELRIYPAMEIEKTSD
                     VDADIRAGINRLVPILEEGIRSALEQWVMFQRVWPETTPEAVKIFPTGSPLESELLEK
                     VASHLPERKSGETRVRVGSFARASSESEAGHD"
     gene            complement(6659..7150)
                     /locus_tag="VGR29_01125"
     CDS             complement(6659..7150)
                     /locus_tag="VGR29_01125"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEV2072216.1"
                     /translation="MAANSSFDIVSKFDRQELKNAIDQAEREIRTRYDLKDTKTEIVW
                     DDNQITITTASEYSLQAVKDTLESKIINRKLSLKILDYQEPEEASGARMRQVIKLKEG
                     IADEVAKKITKQIRDEFKKVTPQIQGDAIRVQAKSRDDLQAVIQMLNGADYPVALQFV
                     NYR"
     gene            7375..8517
                     /locus_tag="VGR29_01130"
     CDS             7375..8517
                     /locus_tag="VGR29_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV2072217.1"
                     /translation="MESVQSDPAHPEREESASGKSAALQEQLLSLPILYKVLIANALI
                     VLAGAVFGTYVTARFVHEDIVPRREGLMLLFGGLGVVLSLAVNYWVLRVAFEPLDSLE
                     RVASAVRHGDFSARTEPWRFGDPQLSRFADTFNETLDELQQDREQIQSLADQVIQAQE
                     EERRRISRELHDDTAQVLFAQLLRVTALKHSSDPELRATAEELEDLTVEAIESVRRLA
                     LELRPPALDDLGLHDALADLAQRFEAQLAIPISLKMSGARGRLASEVELVLYRVAQEA
                     LTNAGKHARATRIVLELVRSDTEVTMRIEDDGRGFDTALAWARDGRGIGLGLFGMEER
                     VGLVGGTLSIDRMDPHGTRVAARIPLSGDTPALSGASRRRRALDVS"
     gene            8514..9197
                     /locus_tag="VGR29_01135"
     CDS             8514..9197
                     /locus_tag="VGR29_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2072218.1"
                     /translation="MTSNTPPGTEPIRVLLADDHGIVRAGLRALLEAQPDMTVVGEAE
                     DGPSAIAQVQELRPTVVIADLSMPGGGLEAIQGITDLDQPTRVLVLTVHAEERYLLPV
                     LKAGGSGYVRKSSAHTDLLNAIRTVARGEVFLDPAATKTLLRGYLGRVRSGDELDLGE
                     VLSEREREVVKLTAEGHTAQQAADILSLSPKTVETYRHRAMQKLGLTNRAELVRYALR
                     AGLLVDDSL"
     gene            9469..9990
                     /locus_tag="VGR29_01140"
     CDS             9469..9990
                     /locus_tag="VGR29_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV2072219.1"
                     /translation="MTKTAVHPGWLLLDDAVTVPGEAGESEDSLRFAAFMQLLRTAGA
                     LDRAATDALADLDLTAGAFGALMELRNVGDAGVAPSELARRLAVARRTATLYVDILAR
                     QGWASREAHPQDRRMVLARLTPEGEALLDELGDAYQRRLASLLGDMSPLQAERLRQLL
                     ALVPTDRPLAVNS"
     gene            complement(10071..12275)
                     /gene="glgX"
                     /locus_tag="VGR29_01145"
     CDS             complement(10071..12275)
                     /gene="glgX"
                     /locus_tag="VGR29_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEV2072220.1"
                     /translation="MTGSTGELHLMPLPLEAQRIRPGTPRPLGATWDGEGTNFAVFSA
                     HATRVDICLFEDSNDKPSRTIELPERTYGIWHGYVPGVAPGTRYGVRVDGPYKPELGE
                     RFNPAKLLVDPYTKAIDGKVTWDDSVYAYDLDDPEDDLSISTVENDASIPKGMVIDPT
                     FDWGDDEPLRTSWYDTVIYEVHVKGFTRQHPDIPEDIRGTYKAMGHPAAIEHLKGLGI
                     TAVELLPVHSFVDDHFLTQKGLVNYWGYSSLGFFAPDKRYSSDGTPGGQVTEFKEMVK
                     ALHAAGIEVILDVVYNHTCEGNHLGPMLSFKGIDNTNYYRLMPDKPRYYIDFTGTGNS
                     LNAHHSQVLTMMMDSLRYWVTEMHVDGFRFDLASTLGRDQYDFDPFGGFFDAVHQDPV
                     LATVKLIAEPWDIGEGGYQVGGFPVLWSEWNDRFRDDVRAFWQTQEPVLPQMGFRLTG
                     SADIYEAGGRSPQASVNLITAHDGFTLNDLVSYAEKHNEANGENNEDGHDHNVSANYG
                     HEGPTDDPEIRALRERQKRNMLATLLFSQGTPMICGGDELGRTQQGNNNAYCQDNEIS
                     WYDWDLTEDQKDLIEFTANAIRLRQDQLALRRRLFFKGRPEQPGSIKDVTWLRPDGQE
                     FQSHDWSDPELRTIGMRLAGDAIQEIDEEGNPLTPSSVLLLFHAGENPIDFTLPRVER
                     GDDLSHWTAMLTSDTPDGSISLKAKALSTITLPGRTVMVFVPSTSEDGSSRQ"
     gene            complement(12340..12531)
                     /locus_tag="VGR29_01150"
     CDS             complement(12340..12531)
                     /locus_tag="VGR29_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072221.1"
                     /translation="MRYEVRYMVDGQEESMVVDVETAAEAAEAARQEHASHGENFELI
                     QVHLLDDLAPDHLEAISEF"
     gene            complement(12544..>13109)
                     /locus_tag="VGR29_01155"
     CDS             complement(12544..>13109)
                     /locus_tag="VGR29_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072222.1"
                     /translation="IGLMLLLLVTVVGVSKVTVEDIDLGEAAPPSASLTVDEETYYRF
                     VAPRLDRMVVEVDRVADMVNGKSRDIIALTISANRIETLSEAIVEFGEQNGVPERFGY
                     VHHCILQSTGTFAYAFDEARSALSRFDFSAMTVLVPQFNDAAHMLHLAQEEMTSIAGS
                     DSALFPAGYVTGSAPGRKSALSTAPID"
BASE COUNT         2524 a         3741 c         4002 g         2842 t
ORIGIN      
        1 attgccgcca actggccagc ctgctccggg gatacgttgg cctcctgctc ggcgaggccg
       61 gaaagcccgg cgcggtccag gagctcgtag aacgtcgtcc actgctccgt ggtctgatca
      121 agctcgacgt ccatgtagag cacggactcg gctggcacaa catcggcggg cgatgtctcg
      181 gcctgcgcaa aggcgcggct agtggggccg tttgcggcga tcacagcaga aaagaagctc
      241 gcgatcacaa agagtgacac gaggaacgaa aaggtccgcc gttgaccgaa tggcttcgcg
      301 gtggttcctg tgtagttctt caaggaaaac tccttcatca gccaacgatg cgtcgaccga
      361 gacaactatg cgagagtccg aaacgctcac ggttaaccgc tgaggacacg aaacgcccaa
      421 gggccgttgg ccaaacggcc atccaggaga gccagctgtc cctcattatc aacgtcgggg
      481 aacaggcaaa tggttccgct cctggtccgc aggttaccga tggacccgct tgtcaaagct
      541 cgtcgccaac gtgaagacga gcatgaaaag cgccgcaatc gccaggatga cgagcaccac
      601 aaaccccact gtaagcaccg cccggatgag accctccaac agttccatag ccgttatccc
      661 ttcgtcgccg ggttggccga ccgatccgcg tcgcgcctta tcccagtata gcggtggatc
      721 gttcgctgag ggttgaggga ttacgtcgtg aggtcacaaa caaaaagggg agctcgggat
      781 ccatccggag ctccctttcc cggtcgtcag gcgacgacct gcgcgttgct gagattgccg
      841 ttgaaggtga actcgtggtt ccgcgcattg acagtgaccg tttcgccttc gcgaatctcg
      901 ccgttgagca acttcaatgc gagcggatca aggatttgct tctggatcac tcgcttcagc
      961 ggcctcgccc cgaacatcgg gtcatagccg agcgtcgcga tgaggtcctt cgccgcgggg
     1021 gtaagcatga gcgagatatc gcggtctctc agtcgtttgc tgacctgctc aagctgaatc
     1081 tggacgatgt cgccaatctg ctccttcgtc agctgccgga agatgatggt ctcatctatc
     1141 cggttgagaa actccggccg gaagttggcg tgcagcgcat ccatcacctg gtgacgaatc
     1201 tcctcatcct gaccctgacc gagcccggcg atgaactggg agccgagatt cgaggtcata
     1261 atgatgaccg tgttcttgaa gtcgaccgtt cttccctggc tgtctgtgac acgtccatca
     1321 tccaggatct ggagcaggac attgaagacg tcagggtgag ccttctcgat ctcatcgaag
     1381 aggaccacgc tgtagggacg ccggcggatc gcttccgtca gttgtccgcc ttcgtcgtaa
     1441 ccaacgtatc ccggaggagc cccaatgaga cggcttaccg aatgcttctc catatactcg
     1501 gacatgtcga tgcggaccat ggcctgatcg ctatcaaaca tgaacccggc aagcgctcgg
     1561 gccagctctg ttttaccaac gccggtggga ccaaggaaga tgaaactccc aagaggccgg
     1621 tttggatctt gcagtccggc gcgggcccgc cgaatcgcgt tggccactgc ctcgagtgcc
     1681 tcatcctgcc cgacgacgcg tgcatgcagg cggtcttcca tctggacgag cttctggatt
     1741 tcgccctcca tcaggcgggt cgacggaatg ccagtccacc ggcttaccac ttcggcgatg
     1801 tcctcggcgt caacctcctc cttgaggagc ttgcctccgg actggaggcg gatcaagtcc
     1861 tgctccagct cattcagcct ggtgtccagc tccttgagct tgccgtattg cagctctgcc
     1921 gcgcggttgt agtcgtaggc ccgctgtgcc tgttcgatct cgacacgagt gcggtccatc
     1981 tcatcccgga gctccgagat ggcggaaatc gcctgccgct cctgatccca acgggaacga
     2041 agcgcgtcgc gctcttcctt caggtcggcg agctcctgct ccagcgatgc aagacgttgc
     2101 tttgatgcga catccgcttc cttgcggagt gcctcgcgtt cgatctcgag ctgcatgatc
     2161 cgacgttccg tttcgtccag ctcaactggc atggacgtga tctccatacg gagcttggat
     2221 gccgcttcgt ccacgaggtc gatggccttg tccggcagga accggtccgt gatgtaacgg
     2281 ttggacagcg tggcggcggc gacgagggcg gagtcaagga tcttgacctt gtgatggacc
     2341 tcgtagcgct cccttaggcc acggagaatc gagatggtgt cctcaacggt cggctcgccg
     2401 accatcactg gctggaagcg acgctcgagg gcggcatcct tctcgatgta cttgcggtat
     2461 tcgttgagtg tcgttgcgcc gatggcatgg agttcgccac gtgccaagag cggcttgagc
     2521 atgttcgacg catccatcga tccttccgcg gcaccggcgc ccaccacggt gtggagctcg
     2581 tcgataaaga ggaggatctc gccgttggag gacgtgacct ccttaagcac tgccttcagg
     2641 cgctcctcga actcaccgcg gaactttgcg cccgccacga gggcaccgag atccagcgag
     2701 atgatgcgct tgttctccag gccctcgggc acgtcgcccc gcacaatacg ctgggcgagt
     2761 ccctcggcga tggccgtctt gcccacgccc ggttcaccga tcaggacagg gttattcttg
     2821 gttcggcggg agagaacctg gatgacgcga cgaatttcct catcacgccc aataacagga
     2881 tcgagcttgc cgtcccgggc caactcggtg agatcacgcc cgtatttctc aagtgcctga
     2941 tacgtactct cggggttagg atcggtcacg cgctggttcc cccggatgtc ggtcaggatt
     3001 cccagaacgc ggtcgcgatc aatgttatac cggcggagca aggactcggc tggcgaccgc
     3061 tcgacggaca tgatgccgag caacagatgc tccgcactga tgtattcgtc gccaaactgc
     3121 cgcgcctcgg tttcagcagc ttgaaggacg cggcgcatac ctgcgcttat gattgcatcg
     3181 gagctgaact gcagctttgg agcggcatcg atgagttttt ccagctcgct cgcaatgatt
     3241 ttcgggtcga tcgaaagtcg ctgcagaatc tgaggaacga ttccctcggc ctggctgaca
     3301 agggagtaga ggagcacctc gggctcgtat tgtgccagct tgcgttgctc cgtaatctgc
     3361 tggccctcga caatcgcttc gcgggccttt tcggtgtatt gctgatttgc tgccataggg
     3421 cggcctcctg tgaagttatc tggaatcgtc aaaggtgtcc cgcccgtcct aaacgcaaga
     3481 cataggacga gggacgtgcg aagatgtcct caaatagttc aggttctgcg ctttcgaagt
     3541 tcctgctggt cgacgtgtcc cagcatgcgc agtaggcggt tatgctccgc ttcgaggctt
     3601 accccgtttg ccttgccacg ggagcttgcc tcccgaaggg cctgcaattc ccgcgtcagg
     3661 tcgagtgcga gctgtacacc tgcgagattc agtcctcgct cctcgacgag atacttcacc
     3721 tgtcggagtt gctcgatgtc ctggtccgag tacaaccgga ggtttccgga cgtccgggat
     3781 ggcgcgatga ggccctcccg ctcgtacttc ctgagcgttt gcgggtgcat ctcgacaagc
     3841 cgagcggcga cgctgatgac aaaaagcggt tctgaaaatc gttgtggcat gtggtcggcc
     3901 tccagagaat gcgtacatat gaatcatata ggctgatatg actcatgtca agaaaagaac
     3961 ccaataattg gagagaatcg tgaaaggata gatccatgac ccctgtgtat gctcatcgag
     4021 gggcatcggc cgagtatccg gagaacacgt tagcggcctt ccgccgggcg ttggagctgc
     4081 acactgaagg catcgagctg gacgttcatt tgtctgcgga tggtgttccg gtggtcattc
     4141 atgacttgaa cgttgaccgc acgacagatg ggtctggcct cgtgagcgag atgacctttg
     4201 acgagctcca gtccctggat gccggccagg gtgaaaagat cccggcgctg gggcaagtgc
     4261 ttgacctggt tggtgacaga ccccatgtgg atattgaggt gaaggccaat gaggcaggtg
     4321 aggcggtcct gagggagtta caaggccgca atacccggtg gctcatatcg tcattcgatt
     4381 gggatgtcct ccactatgtg cgaaggcagg atgccgatgc tgtgctctgg ccactcacaa
     4441 tcgctgcgac ggacgaggca attaatgtgg ccagggatct cggtgctcca gcactggcaa
     4501 tcctgcacaa ggcaatcgat caggacattg tcgagcatct gaaggagcag ggactcgact
     4561 tttgggtgtg gacggttaac gatccggagc aggcacggat atatgccgaa tggggtgcga
     4621 tcggtatctg caccgacgat cctgccgcga tccagcaggg attgggggaa cgccatcccc
     4681 ggacatgaga ggggccaggt ggctaggacc ccgtgctaca ctccacccgt gttgatggac
     4741 ggaggcggac gaaaggcgtg gggtggactg gtgagggcac gtcctgcttg gggaaacgtc
     4801 cgtggctaac gcgataacca tcggccggtt ggtgctactc ttcgtcgtga tctggctgat
     4861 ttacgtcggc aatgtcacga tcattgctgc gtgcatgctc ctgctgatgc tcgtcttcgc
     4921 tgccgatggt gtcgatggct gggttgcccg caaacaggga acgacttcca cttttggggc
     4981 ggtgttcgac attgccggtg acaggatggt ggaaaactct ctttgggtga ttttcgctca
     5041 tctcgatgtc attccagtat gggttccgtt gctcgtcctt agccgcggct tcatcgtcga
     5101 cggtctgcga tcgctttcct ttgccgaggg aatgacggcc ttcggcgaga agaacatgat
     5161 gcgttcgccg atcacgcaat ggctcactgc agggcgactc atgcgcgcgc tcttcggcta
     5221 tatgaaggcg gccggttttg tcttccttac gggcctggtg gcttgggaga caaaagatac
     5281 aaacggaacg gcgctgggtg ctctctacag tcagcagtgg ttccgctggt ttggctggct
     5341 tctggtttgg ggagcagtgg cgctgacagt cgttcggggg ctccctgtgc tctgggacgc
     5401 ctttccctat atgcgcgagc tcgaccggcg aaagcgtgcc ggatgacgca gatttcgcct
     5461 gatccaattc ctgcaggatg acgggccgcc aggtggcggc aagacggatt gtgatcgtct
     5521 gttgaagaga ctgttcttca agagatggga gtactggtta cttggcgcac gacgacaagc
     5581 cgaagagaag aaccgcggtt tttcgcaagg aagaccggca cgagttgcgc taccagctca
     5641 gcgttgccat cgccgcatca ctttcctggt ttttcttcct tgtgccggcg ccaattcgga
     5701 actccttcgc cgagcgttgc gggtcacttt tcttccgtgt ctcacacaca taccgggata
     5761 acgtcatctc caacgtgatg caggtgttgg gtaagacgga ggaggacgcc gaagtgcgtg
     5821 ctactgcccg atcgattttc ctgaacagcg cgctcaactt catggatctc ctgacgctga
     5881 cacgtcgttc cggccgatac ctcatgcgct cgacacatgt tgtggctggt gactggtcaa
     5941 ctatcaacaa tgccatggca agcgggaggg gcttgatcct tgtcacggcg cacgtagggt
     6001 gcttcgactt tattggccaa tcattcctgg ctcacggatt gaaactcacc attgtgacgg
     6061 gcaggacaac atcccgcttt atcttcgatg gggtgacatg gctgaggagc gttcgggggg
     6121 gcaatttcgt tgagcccacc ccttcagggg tgcgcgccgt gatcaaggcg ctccggcggg
     6181 gtgagtgcgc tgtttttgtt gctgaccggg acttcttcca gaacgggcga cccgcgacgt
     6241 tcttcggacg agagacgacg ttgccgccgg gtgttatccg cattgcgcgc gataccgggg
     6301 cggtgatcgt gccgatcttc acacgccgga tgcgacgcgg gcacgaattg cgaatctacc
     6361 cggcgatgga gatcgagaag acgtccgatg tcgatgctga catcagggcc ggcatcaaca
     6421 ggcttgtccc tatcctggaa gagggaatcc ggtcggcact tgaacagtgg gtgatgttcc
     6481 agcgtgtttg gccggagaca acaccggaag cggtgaagat cttcccgacc ggatcaccgc
     6541 tcgaaagcga gcttctggag aaagtggcct cgcatctgcc agagcgtaaa tcaggggaga
     6601 cccgagtacg cgttgggtcg ttcgctcgag cgtcttcaga gtccgaggcc gggcacgact
     6661 agcggtagtt gacgaattgg agcgccacgg gatagtcggc gccgttgagc atctggatta
     6721 ccgcctggag atcgtctcta ctcttggcct ggacgcggat cgcgtcgccc tggatctgcg
     6781 gggtaacctt cttgaactca tcgcggatct gcttggtgat cttctttgcc acttcgtccg
     6841 caatgccttc cttcaacttg atcacctgcc gcatacgggc gccggatgcc tcctcgggtt
     6901 cctggtagtc caaaatctta agcgacagct tgcggttgat gatctttgac tcaagcgtgt
     6961 ccttgactgc ctgaagcgag tactcactcg cggtcgtgat agtgatctgg ttgtcgtccc
     7021 aaactatctc ggttttcgtg tccttgaggt catagcgggt acgaatctcg cgctcggcct
     7081 ggtctatcgc attcttcagt tcctggcgat cgaacttcga gacgatatcg aatgaggaat
     7141 tagctgccat acaccttctc cttacactat ctgcatatcg gtcgggattg taccaaaatg
     7201 accagtactg ctctcgctgg ggttcttagt cggcgcgaga aggatcaagg tgcctgatca
     7261 ttggagaagg cacgagaagg cagcgccgcc ctcggccagc acggagaggg gattcgttcg
     7321 ttgcgtcgat gtggccccag aagttcgcaa acctttcaaa ctaccggaga ggccatggaa
     7381 tcagtgcaat ccgatcctgc acatcctgaa cgggaggagt cggccagcgg gaaatcggct
     7441 gctctccagg aacagctgct ctcgcttccg attctctaca aggtgctcat cgcgaacgcg
     7501 ctgatcgtac tagcaggcgc cgttttcggt acctatgtga ccgcgcggtt cgtgcatgag
     7561 gacattgttc cgaggcgtga agggctgatg ctgctatttg gaggactcgg ggttgtgctg
     7621 agcctggcag tcaattactg ggtgttgaga gtcgcattcg agccattgga tagtctggag
     7681 cgtgtcgcgt cggccgtccg ccatggggac ttttccgcgc gcacggagcc atggcgtttc
     7741 ggtgatccgc aactgtctcg attcgccgac accttcaacg agacgctcga tgagctgcaa
     7801 caggatcgtg agcagattca atcccttgca gaccaggtta tccaggcgca ggaggaggag
     7861 cgacggcgga tatcgcggga gctgcatgac gataccgcgc aggtcttgtt tgctcagttg
     7921 ctccgggtca ctgcgcttaa gcactcctcg gatccggagc ttcgcgcaac ggcggaggag
     7981 ttggaggacc tcaccgtcga ggcgatcgag agcgtacggc gattggcgct tgagcttcgt
     8041 ccacccgcgc tggacgatct tggcctccat gacgccctgg cggatcttgc ccagcgattc
     8101 gaggcccagc tcgctatccc catctcgctg aagatgagcg gtgcccgcgg ccggctcgca
     8161 agcgaggtgg aacttgtgct gtacagggtt gcgcaggaag cgctcacgaa cgctggaaag
     8221 cacgcccgag ccacgcggat tgtgctggag ctcgtccgga gcgatacaga agtgacgatg
     8281 cgtattgagg acgatggacg ggggttcgac acggcgctcg cctgggcgag ggacggcagg
     8341 ggtattggcc tcggcctctt cgggatggaa gaacgggtcg ggctcgtggg tggtacctta
     8401 tcgatagacc gcatggatcc gcatggaacg cgggtcgccg ctcgcattcc gctcagtgga
     8461 gatactccgg cactttccgg ggcgagccga cgtagaaggg cattggacgt ttcgtgacaa
     8521 gtaatacgcc gcctggtaca gagccgatcc gtgtgcttct cgccgatgac catggcattg
     8581 tgcgggccgg tctcagagcc cttctcgaag cccagcccga catgaccgtg gttggcgagg
     8641 ccgaggatgg accgtcggcg atcgcgcagg tgcaggagct tcggcccacc gtcgtcattg
     8701 ccgacctctc catgccgggc ggcggtctcg aggcaatcca ggggatcacc gatttggacc
     8761 agccgacgcg cgtccttgtc ctcacggtgc atgcagaaga gcggtatctc ttgccggtgc
     8821 tcaaggcggg gggatcgggt tacgtcagga aatcgagtgc ccacacggat ctcctgaacg
     8881 ccattcgcac cgtcgcccgg ggagaggtct tcctcgatcc cgcagccacc aagacccttc
     8941 tccgcgggta tttggggcgt gtccgctcgg gagacgaact cgatctcggc gaggtgcttt
     9001 ccgagcggga acgcgaggtg gtcaaattga cggcggaggg tcacacggcc cagcaggccg
     9061 ccgacatcct gtcgctttcc ccgaaaacgg tcgaaacata ccggcacagg gcgatgcaga
     9121 agcttgggct cactaaccgg gcggagctcg tgcgttacgc cctgcgggct ggtttgttgg
     9181 tcgacgactc attgtagcgg gtcaggaaat ccctgacagg cgtgggcaaa agcttgatgg
     9241 tttccacccc gtatttcagg tggtacctgt ctatgcagcg taccatgaac ccgtacactg
     9301 gttgcatgag ggcgatgtat ggtgatacat agcccgtgga gcatcccgga cataattaca
     9361 tgttgtcatc aggcaatctg aaggcgcggt ccaggaattc gctctttagt gtcgtactcc
     9421 cgggctcttg cattcccggg ttgaaggcaa agacagatgg tttcccaaat gaccaagacc
     9481 gctgtacatc ctggctggct ccttcttgac gacgcggtta ccgtccccgg tgaagctgga
     9541 gaatccgagg attccctgag gttcgccgcg tttatgcagt tgttgcgaac ggcgggcgcg
     9601 cttgaccggg cggcgactga tgcactcgcc gatctcgatc tgactgccgg cgcctttggc
     9661 gcgctgatgg agctaagaaa cgtgggtgac gcaggcgtgg ccccatccga gctggcacga
     9721 cggctcgctg ttgcacgccg caccgccacc ctgtacgtcg atattcttgc ccgtcagggc
     9781 tgggcttcgc gggaggcgca tccccaggac cgccgcatgg tattggctcg gctcacgcct
     9841 gagggcgagg cgttgctgga cgagcttgga gatgcctatc agcgacgact tgcgtcgttg
     9901 ctgggcgaca tgagccccct gcaggcggag cgccttcggc agttacttgc actggtgcca
     9961 acggaccgtc cgctcgctgt caattcctag cagattcgtc catatgtcgg atgaatggaa
    10021 aaccatggcg aggagttcac tcctcgccat ggttttctat tggatgctgc ttattggcgg
    10081 gacgacccgt cctcagacgt ggaggggaca aagaccatca cggtgcgacc cggcaatgtg
    10141 atcgtggaaa gcgccttcgc cttcagggag atcgatccat cgggggtatc cgaggtcagc
    10201 attgcggtcc agtggctcag atcgtctccc cgctccacgc gaggcagcgt gaagtcaatc
    10261 ggattctctc ctgcgtgaaa gaggagcaaa actgacgagg gcgtgagggg attcccttcc
    10321 tcgtcgattt cctggatggc atcacccgcc aggcgcatcc cgatcgtgcg tagctcagga
    10381 tccgaccagt cgtgggactg gaactcctgt ccgtcggggc ggagccacgt gacgtccttg
    10441 atacttcccg gctgctcggg ccgccccttg aagaagaggc gacggcgaag agcgagctgg
    10501 tcctgacgga gccggatggc attggcggtg aactcgatca gatccttctg gtcctcggtg
    10561 agatcccagt cgtaccaaga gatctcattg tcttggcaat aggcattgtt gttaccctgc
    10621 tgcgtccgcc caagctcgtc cccgccgcaa atcattgggg tgccctgcga gaagaggagg
    10681 gtcgccagca tgttgcgctt ttggcgctcc cggagcgccc tgatctccgg atcgtcagtg
    10741 ggaccttcgt gtccatagtt ggctgaaacg ttgtgatcgt ggccgtcctc gttgttctcg
    10801 ccgtttgcct cgttgtgctt ttccgcgtac gagacgagat cgttcagggt gaacccatcg
    10861 tgtgcggtaa tgaggttgac gctggcctgg ggtgatcgac ctcccgcttc gtagatgtcc
    10921 gccgatccgg tgaggcggaa ccccatttgc ggtagcaccg gctcctgcgt ctgccagaat
    10981 gcgcggacgt catcacgaaa ccggtcattc cactccgacc aaagcaccgg aaacccgccg
    11041 acctggtacc cgccttcgcc aatatcccac ggctcagcga taagcttcac ggtcgccagc
    11101 acaggatcct ggtggaccgc atcaaagaac ccgccgaagg gatcgaagtc atactgatcg
    11161 cgtccaagcg tgcttgccag gtcgaaccgg aacccgtcaa cgtgcatctc ggtcacccag
    11221 tagcggaggc tgtccatcat catggtcagc acctgggagt gatgcgcgtt caggctgttg
    11281 ccggtgccgg tgaagtcgat atagtagcgc ggcttgtccg gcatcagccg atagtagttg
    11341 gtgttgtcaa ttcccttgaa cgacaacatc ggcccgaggt ggttcccttc gcaggtgtgg
    11401 ttgtagacga catcgaggat cacctcgatg ccagcagcat gaagcgcctt gaccatttcc
    11461 ttgaattcgg tgacctggcc gccaggagtc ccgtctgatg agtaccgctt atcgggggca
    11521 aaaaaaccga ggctgctata gccccagtag ttgacgagcc ccttctgtgt cagaaagtgg
    11581 tcatcgacaa atgagtgaac cgggagaagc tcgacagcgg tgatcccgag tcccttcaga
    11641 tgctcgattg ccgcagggtg tcccatagct ttgtaggtgc cgcgaatatc ttccgggatg
    11701 tccggatgct gccgcgtgaa gcccttaaca tgaacctcat agatgacggt gtcataccag
    11761 gaggtacgca acggctcgtc gtctccccaa tcaaacgtgg gatcgatcac catgcccttg
    11821 ggaatgctgg catcgttctc cacagtgctg atggagagat cgtcttcggg atcatccaag
    11881 tcatacgcgt aaacggagtc gtcccacgtc accttgccat caatggcttt ggtgtatgga
    11941 tcgaccagca gtttcgcggg attaaaccgt tccccgagtt ccggcttgta cgggccatca
    12001 acacgtacgc catagcgcgt tccgggggcc acgccgggta catatccgtg ccagatacca
    12061 tacgtgcgtt cgggcagctc gatcgtgcgg ctcggtttgt cgttcgagtc ctcgaagaga
    12121 cagatgtcta ctcgggttgc atgcgctgag aagacggcaa aattggtccc ctcgccatcc
    12181 caggtggcgc cgaggggacg tggagtgccg ggacggattc tctgcgcttc cagcggaagc
    12241 ggcataaggt ggagttctcc agtggaccct gtcattcagg ctatagaatc attcgggcag
    12301 acgtaaaacg gaaacctgac tggttcagtt tcgagcaagc tagaactcgc tgattgcttc
    12361 caggtggtca ggagcgaggt catcgagcag atgtacctga atcagctcaa agttctcgcc
    12421 gtggctcgcg tgctcctgcc gtgccgcctc agcggcttct gctgccgtct ctacatcgac
    12481 gaccatggac tcctcctgcc cgtcaaccat gtatctgact tcgtatctca tgtgatcgtg
    12541 tcctcagtcg attggcgcgg ttgaaagagc agacttccgc ccgggagcgg agccggtcac
    12601 gtaccctgcc gggaagaggg cagaatcgct gccagcgatt gaagtcatct cctcctgcgc
    12661 gagatgcagc atgtgggctg cgtcgttgaa ttgggggacc aataccgtca ttgccgaaaa
    12721 gtcgaagcgg ctcagcgccg agcgagcctc atcaaaagca tatgcgaagg taccggtact
    12781 ctggaggatg caatggtgaa catacccgaa tcgctcgggt accccattct gttcaccaaa
    12841 ctcgacaatt gcctccgaga gtgtctctat gcgattggca ctgattgtca acgcgatgat
    12901 gtcgcggctc ttgccgttga ccatatcggc tacgcgatca acctctacca ccatcctgtc
    12961 gagccggggc gcgacaaacc tataatacgt ctcctcgtca actgtaaggc tggctgacgg
    13021 tggagcagct tcgccgagat cgatatcttc gacggtaacc ttcgacacgc caactaccgt
    13081 aacgaggagc agcaacatta atccaatca
//
LOCUS       DASYWM010000029         5940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_413886, whole genome shotgun sequence.
ACCESSION   DASYWM010000029 DASYWM010000000
VERSION     DASYWM010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413886"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VGR29_01160"
     CDS             complement(<1..217)
                     /locus_tag="VGR29_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2072223.1"
                     /translation="MQGQRIGQSDLEVSAIGLGTWPMGDARYGPRDDHEAERTITTAL
                     DAGVTCFDTAPIYGNGHAEELLGKALTG"
     gene            671..2632
                     /locus_tag="VGR29_01165"
     CDS             671..2632
                     /locus_tag="VGR29_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2072224.1"
                     /translation="MTKWISSQPGPRVLSRRRFIGGVMATAMTAAYGRQAQTARAASA
                     AVTISRGSTSNAVVALTFDCGADSGYTGSILDTLANRGVRASFGVTGKFAASYPSLVK
                     RMVNEGHSLINHSYSHPSFSGLTTTQRLSELESTEQALVNASGRAGRPLFRPPYGEFN
                     SSVLTDLGNAGFTYNIMWSIDVLGWNGLTRDQVINRALSNHGNGYIYLMHVGGQSQEG
                     PALPSIIDGLRSRGYGFVTIPQMLGGTSAPPPPSPPATGFNIGDTVKVTAGLYLRTAP
                     TLSSGVITTMPTGTVCTIVGGPTSADGYTWYQVKTTYGTGWAAGQYLQLTTSTPPPAT
                     PGGYAAGTKVRVTSGLWLRTAPNLSGGVITTMPTGTICTVLSGPSPSGGYTWYQLDTP
                     YGQGWAAGEYLEVTTQSPPPPTTTPVGYAAGTKLSVTSALYLRASASLSSGVITTMPV
                     GTIVTVVSGPSPSGGYTWYQVDTPYGRGWAAGEFLKPATTAPPSTTEPPPPTTGGHAA
                     GTTLRVISGLWLRTAGNLSGGVITTKPTGTIVTVVSGPSPSGGYTWYQLDTPYGRGWA
                     AGEFLEPTTQSPPPVTSGYPAGTKLRVTAGLWLRTAGNLSGSVITTMPTGTIVTVVSG
                     PSPSGGYTWYQLDTPYGRGWAAGEFLVRA"
     gene            complement(2712..3509)
                     /locus_tag="VGR29_01170"
     CDS             complement(2712..3509)
                     /locus_tag="VGR29_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2072225.1"
                     /translation="MSARPSSLAIYTQHLTGRFRRTGIFWAIGLGVYIALLILSYPSF
                     RESGLLALENYPKTIIEAFGIEDLTTIGPFLDVRVYSSLPLVISFFPVMTFAGALAGA
                     EERGSLDILLGNPIPRRHVVLANWLALALVLLAVLLVVAIVSWGCGRLINVGLDAADA
                     LRASLNLFPITMAFGTLALALSAWLRSRGAVIGISFAVMFLMYLLETIGRISPTWAGL
                     RWVSAFRLYGSAITDGIDWGNAALLLGASVVLLVLAIGLFDRRDIYT"
     gene            complement(3506..4420)
                     /locus_tag="VGR29_01175"
     CDS             complement(3506..4420)
                     /locus_tag="VGR29_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2072226.1"
                     /translation="MTVAIVTYNLTKTYGANRGIRNINLSIQQGEIFGFLGPNGAGKS
                     TTLRTLIGLMKPTSGRAEILGLDPHTQTTEVLARIGNLPTEFALDDRMTGRRMLSLFG
                     RLRGLTELAYADELAERLDADLDRPMRRLSRGNKQKIGIIQALYHKPEVVILDEPTGG
                     LDPLVQESFLSLLVEAKGRGQTIFFSSHVLSDIERVADRVGIIRHGQMVAVEDPHELT
                     GRAHRQVRVEFATPMTDDMCQRLAGIPGVENVAFESTIARFTVSENIDAVIKLLGEHQ
                     VRTLDVERPSLEEIFMTFYGPEENGDAA"
     gene            complement(4773..4958)
                     /locus_tag="VGR29_01180"
     CDS             complement(4773..4958)
                     /locus_tag="VGR29_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072227.1"
                     /translation="MSEHDPNQPGEIVNESTPTNEPEPNPEPSLHHTEDQPEEASGKE
                     TGRSEDDQDSDSDEAGR"
     gene            complement(5027..5254)
                     /locus_tag="VGR29_01185"
     CDS             complement(5027..5254)
                     /locus_tag="VGR29_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072228.1"
                     /translation="MPSGTYRYEEIQNVSPTEFGEELTRVLNQLRGDGWEIVSISLSL
                     AKLGPVASDYEHSSRVAQIVAYHPATADSRE"
     gene            complement(5494..>5940)
                     /locus_tag="VGR29_01190"
     CDS             complement(5494..>5940)
                     /locus_tag="VGR29_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072229.1"
                     /translation="RRDRVTPEDYPRPDSFYGWAKAAYESLGFLYASGSLGRKLEVIQ
                     IRIVAPREIRASDFEGKPVEQYIRDITGYISERDLQQLFCKSVETPNIDDAFGVPFHI
                     FYGVSNNARTFWSITNAREVIGYRPEDDSEIRFAEDIARMVSRARS"
BASE COUNT         1157 a         1783 c         1798 g         1202 t
ORIGIN      
        1 gaccggtgag cgccttgccg agcagttcct cggcatgtcc gttaccgtag attggggcgg
       61 tgtcgaagca ggtgacgccg gcatcgagtg ccgtggtgat tgtgcgctca gcctcatggt
      121 cgtcgcgtgg gccgtagcga gcgtcgccca ttggccatgt tcccaggccg atggcggaga
      181 cttcaagatc tgactgaccg attcgttgtc cctgcataag tggttgcgct ccgattacat
      241 gcgcggatcg aggtcgtccc gcaggcggtc gccaaacacg caacgcacgc gaccgacgcg
      301 gctcctgata cttcgggggc cgtggtcgtg cccttcacgc ccgtgcgaga ggccctatat
      361 cagcctttat ggggtgtcgg gatcatccat gagggaaccc gttatcgcgc gtgatgcacg
      421 gcgtttcggt tgggtgatat tcaccatttc accacgctct gtatgctcta gtacacctct
      481 catttgacct gagagtcaac aagaacttgc tcgatccagt tgagtcagtg agattacgga
      541 tatgggcgcg ccataaatca gtaattttac ggatacgact tcgagcacta ttccctaaac
      601 tcggatcaca tcacgttggg gaaagttgtt gggggacctg tcgagcgttt tcatgaagga
      661 gacagatgcc atgacaaagt ggatttcttc tcaaccaggt cctcgtgtcc tctccaggcg
      721 tcggttcatc ggcggcgtga tggcgaccgc gatgaccgcc gcatatggtc gtcaagcaca
      781 aaccgctcga gccgcctctg cggcggtcac catctcgcgt gggtcgacga gcaatgccgt
      841 cgtcgcgctg accttcgact gtggcgccga tagtgggtat accgggagca ttctcgatac
      901 gctggcgaac cggggtgtgc gagccagttt cggcgtgacc ggcaaattcg ctgcctccta
      961 tccttccctg gtcaaacgaa tggtgaacga ggggcacagc ctgatcaacc attcgtattc
     1021 gcatccctcg tttagcggtc tgacaacgac gcagcggctc tctgaactgg agtcgaccga
     1081 gcaggcactg gtcaacgcga gcggacgggc tggacgtcca cttttccggc ccccctatgg
     1141 cgaattcaac agcagcgtgc ttacggatct gggtaatgcc ggattcacgt acaacatcat
     1201 gtggtcgatc gatgtgctgg gctggaacgg gctcaccagg gatcaggtga tcaaccgggc
     1261 gctgagtaac cacggcaacg ggtacattta tctgatgcac gtcggcgggc aatcccagga
     1321 agggccggcg ttaccgagca tcattgacgg cctgcgatcg cgcggatacg ggtttgtcac
     1381 catcccgcaa atgctcggcg gtacctcggc accgcctcca ccatcgccac cggcgaccgg
     1441 gttcaatatt ggcgatacgg tgaaagtcac ggccgggctc tatctgcgga cggcgccgac
     1501 cctcagcagt ggggtgatca cgacgatgcc gaccggaacg gtatgcacga tcgtgggcgg
     1561 gcccacttcc gcagacggct acacctggta tcaggtcaag acgacgtacg ggacgggctg
     1621 ggcggccggg caatatcttc agctcaccac atctaccccg cctcctgcga caccgggagg
     1681 ctatgcggca ggtaccaagg tgcgagtcac gtctggtctc tggttgcgaa ctgccccgaa
     1741 cctgagcggc ggggttatca ccaccatgcc caccggcaca atctgcactg tcctgagtgg
     1801 gcccagcccc tccggtggat acacttggta tcaactcgat acgccctatg gtcaggggtg
     1861 ggctgctggt gaatacctgg aggtcaccac gcaatcgcct ccgccaccga cgaccacacc
     1921 ggttggatat gcggccggta cgaagctctc cgtcacctca gcactctacc ttcgggcctc
     1981 ggcgagcctc agtagtgggg tcattacgac aatgccggtg ggcacgatcg tcaccgtggt
     2041 gtcgggtccc agcccctctg gcggctatac ctggtaccag gttgatacgc cttatggacg
     2101 tgggtgggcc gccggcgagt tcctcaaacc tgctacgaca gcacctccat cgacaaccga
     2161 gccaccaccc ccgacaaccg gcgggcatgc cgcgggaaca acgctacggg tcatcagcgg
     2221 gctttggtta cggacggccg gtaacctgag cggcggggtt atcaccacca agccaaccgg
     2281 cacgatcgtg acggtggtct cgggtcccag tccctctggc ggctatacct ggtatcaact
     2341 cgacacaccg tatggacgtg ggtgggccgc cggtgagttt ctcgagccaa cgacccaatc
     2401 gccgcctccg gtaaccagcg ggtatccggc gggaacaaag cttcgcgtga ctgcgggact
     2461 ctggctgagg acggctggca acctgagtgg gtccgtgatc acgacaatgc cgaccggtac
     2521 gatcgtgact gtcgtatccg ggccgagccc atctggtggc tacacctggt accagcttga
     2581 tactccgtat ggccggggat gggcggccgg cgagtttctt gtcagggcgt gatattccag
     2641 cattggcctc accacctgat gaaggtgggg ccaatgctcc tatctggtca ctgctgcgca
     2701 tcggatgtag cctaggtgta aatgtcccgt cgatcgaaga gcccgattgc cagaaccagg
     2761 agcacgacgg aggcacccag caagagggcc gcgtttcccc aatcgatccc gtcggtgatt
     2821 gcgcttccgt agagtcgaaa tgccgacacc cagcgcagcc ctgcccaggt gggtgaaatc
     2881 ctcccgatcg tttccaacag atacatcagg aacatgaccg cgaacgagat accgatcacc
     2941 gcaccacgac tgcgaagcca ggctgaaagc gcgagggcaa gagtgccgaa cgccatggtg
     3001 attgggaaga ggttcaacga cgcgcgaagc gcatcggctg catccagtcc gacattgatc
     3061 aggcgcccac atccccagct gacgatggcc acgacaagca gtacggccag gagaacgagc
     3121 gcgagggcga gccagttcgc gagaacgaca tggcgacgag ggatcggatt gccgagaagg
     3181 atatccagcg atccacgttc ctcggccccg gcgagcgcgc ccgcaaaggt catcaccggg
     3241 aaaaagctga tcacaagcgg gagcgaggaa tagacgcgta cgtcaaggaa ggggccaatt
     3301 gtcgtcaagt cctcgatccc gaacgcctcg atgatggttt tcggatagtt ttccagcgcc
     3361 aggagtccgg actcgcggaa cgagggatag gacaggatca agagggcgat atagacaccg
     3421 aggccgattg cccagaagat gccggttcgc cggaagcgcc cggtgaggtg ctgggtgtag
     3481 atagccagcg agcttgggcg tgcgctcatg cggcatctcc attctcttcc ggcccataaa
     3541 aggtcatgaa gatctcctca agcgaggggc gctcaacatc cagtgtgcgc acctggtgtt
     3601 cgcccagtaa tttgatcacc gcgtcgatat tctccgatac cgtgaagcgg gcgatcgtgc
     3661 tttcgaatgc gacgttctca acgccgggga tgccggcgag acgttggcac atatcgtccg
     3721 tcatcggcgt agcgaattcg acgcgcactt gccggtgggc acggccagtg agctcgtgcg
     3781 gatcctcgac ggcgaccatc tggccatgcc ggatgatccc gacacgatcg gcaacccgtt
     3841 caatgtccga gaggacatgc gatgagaaga agatggtctg cccacgccct ttcgcctcaa
     3901 ccagcaggga gaggaaggat tcctggacaa gcgggtcgag gccgccggtc ggctcatcga
     3961 gaatcacgac ctcgggcttg tgatagagcg cctggataat gccgatcttc tgcttgttgc
     4021 cgcgagagag ccggcgcatc ggacggtcca ggtcggcatc aagtcgttcc gcgagctcgt
     4081 cagcgtaggc gagctcggtc agccccctga gccggccaaa gagggagagc atccgccggc
     4141 cggtcatccg gtcatccagc gcgaactccg ttgggaggtt accgatccgg gcaagcacct
     4201 cggtggtctg tgtgtgtgga tccagaccca ggatctctgc ccggccactt gttggcttca
     4261 tcaatccgat cagtgtccgc aaagtcgtgg atttgcctgc gccgttcggg ccgaggaagc
     4321 caaagatttc gccttgctga atggaaaggt tgatgttccg gatgccacga ttggcaccgt
     4381 aggtcttggt gaggttgtaa gtcacgattg cgacggtcat gcatcgtctc ctgctcccga
     4441 tcatgataat tggccgctac agttggagcg tgcggacgct gcatgaggtc acaggttgct
     4501 gccacggcag acgcctcaga aaatccctcc ggcatgccga atcaccgctc atcttcagca
     4561 gctattcgtc atcgccaggt tggtgttttc ctttgcttcc cctgattcga ttatcgatgt
     4621 gtcgatccag ttcatggcca ggacgcgtga tcgcccactg gctccacgct tcccaaaacc
     4681 atttcttccc gacgtgttaa gggaaccgga gagctgtcgg gtcgagcgag tcatcactcc
     4741 tcggtggtgt atttccgcgt ctcgcgacgc cgtcagcggc cggcctcgtc actgtcgctg
     4801 tcctggtcgt cctccgatct cccggtttcc ttccctgacg cttcctcggg ctgatcctca
     4861 gtgtgatgca gggatggctc cgggttcggc tcgggttcgt tcgtcggggt ggactcgttc
     4921 acgatctcgc cgggctggtt agggtcgtgc tcgctcatgg attcctcctg gaatacacat
     4981 cgtgacgctc cggagcgtct gtatacgagg atcgatatcg caaggattac tcccgcgagt
     5041 cagcagtcgc cggatgatag gcgacgatct gggcgacccg ggacgagtgc tcgtagtcgc
     5101 ttgctaccgg accgagcttg gcgagcgaca gcgatatgct cacaatctcc cagccgtcac
     5161 cccgcagctg gttgaggacg cgggtcaact cctcaccgaa ctctgtggga ctgacgtttt
     5221 ggatctcttc ataccgatat gtcccggatg gcatgtcgct ccactcccat gcacgtgctg
     5281 ccgcctgggg gaatcgacga cgggtctccc tcgcggcata aaatcaacct ctcctggctg
     5341 tatccggcaa gcttgccctt tggctcctcc tcgatagcgc gcctgaaccc caccacctta
     5401 tcgagtgtca cttccagtcg tccgcttcct ttttaggacc gcgtttagga ccgtggatac
     5461 ccaaagcatg gtggaactga ctgggctgca tctctacgat cgggcacgag aaaccatgcg
     5521 tgcgatatcc tccgcgaatc ggatctcgga gtcgtcctcc ggccgatagc cgatgacctc
     5581 gcgggcgttg gtgatgctcc agaaggtccg cgcgttgttc gatacgccat agaagatgtg
     5641 gaacggcact ccaaaggcat cgtctatgtt cggtgtctcc accgacttgc aaaagagctg
     5701 ctgcagatcc cgctcgctga tgtagccggt gatatcgcgg atgtactgtt ccaccggttt
     5761 tccttcgaaa tcactggcgc gaatctcgcg cggagcgaca atccgaatct ggatgacctc
     5821 cagtttgcgg cccagcgatc cagatgcata gagaaagccg agcgattcat aggcagcctt
     5881 cgcccatccg tagaaggagt ccggccgagg gtaatcctct ggcgtgacgc ggtcacgccg
//
LOCUS       DASYWM010000030        14782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_95003, whole genome shotgun sequence.
ACCESSION   DASYWM010000030 DASYWM010000000
VERSION     DASYWM010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14782
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95003"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VGR29_01195"
     CDS             complement(<1..579)
                     /locus_tag="VGR29_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV2072230.1"
                     /translation="MVETTKTAKTFQDVPSKVDFPQMERDLLEWWDKEQVVRQYLDRN
                     NGAEERFSFIDGPITANNPMGVHHAWGRSYKDIFQRYQNMLGKEQRFQNGFDSQGLHV
                     EVEVEKELGLKNKREIESFGVAEFVERCKQRVWKYSARMTEQSKRLGYFMDWDNSYYT
                     MSDENNYTIWHFLKTCHERGWIYKGDDVMPWCP"
     gene            complement(1037..1750)
                     /locus_tag="VGR29_01200"
     CDS             complement(1037..1750)
                     /locus_tag="VGR29_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2072231.1"
                     /translation="MGLRHFLNTPRSISLNPERVARRRSKRGFIASLTSMVLFSVIFW
                     LVRRNRSAAADAAVTLRVQKQDHPYFDRLMHIVSWPGFPPQSRILPPGIAAALWLRGM
                     RLEAIFQLAAWGTGGVSSTFKRIMRRKRPSSEGTGIKVAVANIGGSSFPSGHVIIYMG
                     VYGFLAYLADTWVRANAIRRVVVALLTSLLALVGPSRIYLGHHWFTDVAASYLLGTTW
                     LVGLTALYRRTKRWMIKGA"
     gene            1859..2662
                     /locus_tag="VGR29_01205"
     CDS             1859..2662
                     /locus_tag="VGR29_01205"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEV2072232.1"
                     /translation="MSTVLVRSIYRYWLWYGVVLAGMAVLMLLPGGFAEKSRMLLHGL
                     CAQAPTHSFTLGGVLLPFDSRMTGIYGGTLATMIYLTARSRFFACGVPPTNVVVVLAL
                     FVIAMAADGFNSLFTDLGIWHPWTPSNELRLITGYLAGITLGAMLSWLLGSALYRVGQ
                     RQAGIQRPIDVLVILVPLFPFGAILLSGAEWLFVPTATLLIVSAWTTMSVLGLSIIVL
                     AFGLDTRINRLRDLHVPGAAAVLLGLAIMLGLAFGRMWLEHFMGMPSTL"
     gene            2865..3644
                     /locus_tag="VGR29_01210"
     CDS             2865..3644
                     /locus_tag="VGR29_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEV2072233.1"
                     /translation="MRIVRHNAHISQRKRRARIFSFFGFLLLSGSLLIAWYPEFLLFA
                     YVAMLGGFILFNMGMQQLGKWTRNPRNDQFLDHQMKGLSDKITLIHYAQFGKHNIEHV
                     VVHPGGLVVLTAREVDGTIEKRGTRWRRKGGLYRRLFSFSGPQIGNPSFETDANVTRA
                     EDFLAEHQMEVDVEGAIVFVHPRTELDIEDPDYPVLHGDEVDEFIRDLPADESFTREE
                     RDRIVELLSAGERVETPQTQKQGARRPRPVRRVAAPKVKPQ"
     gene            3662..4720
                     /locus_tag="VGR29_01215"
     CDS             3662..4720
                     /locus_tag="VGR29_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEV2072234.1"
                     /translation="MTATGIEAPPSSTGWRVWGHDHAVHELRSAIDKGVRHAYILSGY
                     HHVGKTALAQEFARALMCGSSPSPGQPCGVCSACRRVGRGAHPDVTLYDLARQQETSD
                     KASTSKNLSLNIQTVRDITSSISLRPMEARHRVVIVDDVETMQETAQEAFLKTLEEPP
                     PYAVILLLTTDAELLLETIRSRCMTLHLQTVRPSTILDLLLATGLDEKDATAIAAASI
                     GRPGWAVQAASDPNILRERLELESLVKGWITGDQYRRMVEATRLGDVFSKDRAAVYAR
                     LSLAQTMWREIVLHALGVVDVDRPDKNIFEGQQPTAGEGVTALRSVERCLNDLDANVR
                     PKLALQSMVLQWPTIAAR"
     gene            4696..5493
                     /locus_tag="VGR29_01220"
     CDS             4696..5493
                     /locus_tag="VGR29_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072235.1"
                     /translation="MANDRRPMISPKGPKAGPPGPGRTELPDASEDQNGFNIVGVRLF
                     DSPRVVYFLLSRGSVQPGTWVVVRARHGEEAGRVVIAPQQIVLAQISGEIEPIERVMS
                     DEEILQMERFLNESPALVERAREIVRRCWPHVELRSARYSFDGSYVVISLAGSDGDEI
                     LGLEEALAEEFRVPVRIRNAGTRQPTRLMGGLGTFGRKPDTLSRENEHYRKIKEDLPR
                     LGQRVRTHDGEGMVVALQVFRELVTVRYTDGGREMAHPVTDLLPERA"
     gene            5665..5970
                     /locus_tag="VGR29_01225"
     CDS             5665..5970
                     /locus_tag="VGR29_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072236.1"
                     /translation="MPLYLVVHTPRKDDEEKLFPPTKMLDMARDHGAEDIRTRWLKTW
                     SPDLHDERHFTLWSANSAADITEVMSRYNFLSETDSEPICVTEWSPADVLESADAEE"
     gene            6155..8422
                     /locus_tag="VGR29_01230"
     CDS             6155..8422
                     /locus_tag="VGR29_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV2072237.1"
                     /translation="MATAYKTIGQRTRRLDSPPKLTGTEKFTADMSLPGMLHARIVGS
                     DLAHGRILSIDKSAALDMPGVVAVLTNADLPIHRDENDIPAAELLASEEALHVGHPIA
                     LVLAETEAAAQDAAELVVVDMEPMPVVSDLHEAIREDAPRVSADKAGAFEDEAAMHNA
                     DAAGSAEKQEDLPPNVSNSANFERGDIDAGFAEADEIVELSLTSAAIHQGYIEPQVAL
                     AAPDAFDKLTIYTSTQGAFHGRQKVSDTLGMPLDSVNIVPMPVGGGFGGKFVLIEPLV
                     AAAAKAVNRPVLLQYTRSDDFLAGNPAPECAIKIKLGAKRDGTLTALDVDLLFDAGAA
                     SGSPLGIAAILVGGYYRFPNLRIKGYETKTHRPSSGSYRAPGAQQATFAIESAMGELC
                     DRLGMDQIEIRLLNCAEEGDLRPNGGEWPRIGLRQTLEALRDHPAWQQRDAAKAQGHG
                     IGVAVGGWPGGIEPASAACRLDGDGKLTVMLGSVDLNGTNTAFAQIAAEGLGTGVEDV
                     RVQTVDTAGAPFAGGTGGSKITYTVGPAVQKAVEDAVEQIKQIAADHLEASPEDLELV
                     DGGVRVKGVPGSSVTLKEIARMSMSTGKGHMPVQGRGGSGIATQAPGFAAHLAEVSVD
                     DLTGVVTIHRYVAAQDVGFAINPATVEGQIEGGVLQGIGWALYEGMPYDEDGQPGAST
                     LMEHTLPRARMAPPIETLLVEVASEAGPYGAKGVGEPPAIPGAAAIANGIKDATGVRM
                     TALPILPAAVLEQLG"
     gene            8570..10339
                     /gene="recN"
                     /locus_tag="VGR29_01235"
     CDS             8570..10339
                     /gene="recN"
                     /locus_tag="VGR29_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV2072238.1"
                     /translation="MLVELAISDLAIIERTTIHFEEGLNALTGETGAGKSILLDALGA
                     VLGQRVSSDLVRTGAKAARVEAAFDLSGEEGPRVVEILGSYGIEQRLDEPLLLSREIS
                     SAGRSSARINGRMSTVAQLNEIGAILVDIHGQSDHLSILQTSEQRSILDRYARLELLR
                     DEVGVLVREYLEVRRLLEDVSRGSREREQRLDLLRFQLGEIDEAGLASGEDERLIRER
                     EVLQNADRLRQSAMSAVEALVGDESGAVEVSANALLRQAEHAAADLGEIDETAQELSE
                     RATELVVLAEDLVRDLRSYADTIEGDPERLMEVEDRLDLIQTLKRKYGGSIDEILAFR
                     DEADRELQQLTGTGFDREALVAREASISRELASCASLLSEKRSEVSLELSARIVESIS
                     ELRMGSSNVSVEVRQREDADGIELPDGRRVHVDETGIDEVEFMIAPNAGETLKPLGRI
                     ASGGETARVMLAVKSILSAVDETPTLVFDEIDVGVGGRSGQMVGEKLWSLTDRHQVIV
                     ISHLAQIAAFADKHFRIVKEPREGRVVSSVQELDRLTREEELAAMLDGVPATPESLAN
                     ARAMLQRVYDYRSRGMRKQEAIS"
     gene            10407..10709
                     /locus_tag="VGR29_01240"
     CDS             10407..10709
                     /locus_tag="VGR29_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV2072239.1"
                     /translation="MQPNMKMIQQMQNRMMKIQQELEETVVEGTAGGGVVKVEVNGQR
                     AMQSIKIDPSAVDPEDVEMLEDLVVAAVQDAMDRASKLAEDKMGALTGGMKIPGLM"
     gene            10728..10976
                     /locus_tag="VGR29_01245"
     CDS             10728..10976
                     /locus_tag="VGR29_01245"
                     /inference="COORDINATES: protein motif:HMM:NF017879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEV2072240.1"
                     /translation="MILSEPIPFYIDDIVRLRKPHPCGSYEWKVVRIGADIGLVCQEC
                     GRRVLLPRRVLEKRLKAFVSRGPTAPGAPAADDQITSP"
     gene            10988..12406
                     /locus_tag="VGR29_01250"
     CDS             10988..12406
                     /locus_tag="VGR29_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2072241.1"
                     /translation="MRDKNGKRDVRPDSMGAVLRNGAFLRLWLIQATTQILQNMINFA
                     LLLRVRGVVEVHDLPHANTAISLVILAFSLPAVLFGPVAGVIADRVNKRNLMAVLNAA
                     RAVAVLLFLVIRPGWDVETILAAYYIVTFLFGIAGQFFAPVLGATIPLVAKRQQLMSA
                     NALFNLTSTAAQLIGFAAAGPILIRVLGVDRLFAVTVVAYLISVALVFTLPKTPVPVR
                     AINEIELRPMQRLWSDMKEGLVFILQDPALMRAIGYLTLASTTFLMIATLGPDFVSSV
                     IGLPKEDIGFIVAPAGAGILAGVLIVPRIVRRIGRDWLIDISLTIAGLMLALMALTRG
                     VLNGIVPGNAPLWTVVGFTASFAGILGLCNAFILVPSQTMLQERSHEGVRARVYATFF
                     TVSNTVAFVPIFFAAAAADIFGVINVLIVMAFFVGGLGFFGLFHRRRAERNKWERVRT
                     RHRQGPDAIPRRFEGSGKPVKS"
     gene            complement(12456..13361)
                     /gene="pyrB"
                     /locus_tag="VGR29_01255"
     CDS             complement(12456..13361)
                     /gene="pyrB"
                     /locus_tag="VGR29_01255"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEV2072242.1"
                     /translation="MKHIVAVEQFERAWIETLFADADRMRSVTAAEGLLRGKILATLF
                     YEPSTRTRLSFESAMLRLDGQVISTENAREFSSAIKGETVEDTIRIVAGYADCIVIRH
                     FERGAAQRAANASPVPIINGGDGPGEHPTQALLDLYTIQNELGQIDDLKVALVGDLRF
                     GRAARSLALLFRKTRNTELVFVAPGAVPMGDDIKDALDHDSVPWREEPDLTKAMGEVD
                     VVYQTRIQRERFATPEEYTEAQGIYIMTPDNMKDLGEHAIVMHPLPRVDEIHPEVDSD
                     PRAAYFRQARNGVFIRMALLHQLLA"
     gene            13453..14343
                     /locus_tag="VGR29_01260"
     CDS             13453..14343
                     /locus_tag="VGR29_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2072243.1"
                     /translation="MLQQLTERVFVAAGGTNTGVVISDDGRVVLIDTGLNDTHARKVL
                     RAVRYDLGTEVRAILTTHGHADHFGANAFVVKRTSASVHAPDVDEMVLRHPLMQTVML
                     FGGADPVDTLRTRFLLAEDGPVDGLLEPGNQVLEGVELDIVSLAGHSMNQMGIVIDSI
                     FFCADVVFPDSTLEKYRIPYLYGLTEHLDALDFSLSVACRQVVPGHGPVEEGITGPVE
                     RNREVINRTITALLSVLDTPKSTDQICVDLFTALDVPVGDDGGYFLLRPTVAAYLSHL
                     QRVNQVCTEVANKGVVWRRT"
     gene            complement(14510..>14782)
                     /locus_tag="VGR29_01265"
     CDS             complement(14510..>14782)
                     /locus_tag="VGR29_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072244.1"
                     /translation="LEKINPNFDPTWVKQTWVFRERAAQPIIPLNYSEMIPEHRTPLK
                     NLYLANTTQIYPEDRGTNYSVRLGEDIAKLILDDIKCRGAEPIVQE"
BASE COUNT         2880 a         4177 c         4644 g         3081 t
ORIGIN      
        1 ggggcaccag ggcatcacat cgtctccctt gtagatccag cctcgctcgt ggcaggtctt
       61 caggaaatgc cagatggtgt agttgttctc atccgacatt gtgtagtagc tgttgtccca
      121 gtccatgaag tagccgagac gcttggactg ttcggtcatc cgcgctgagt atttccagac
      181 gcgctgcttg catcgctcca cgaactcagc gacaccgaat gattcgatct cgcgcttgtt
      241 cttgaggccc agctccttct ccacctcgac ctcgacatgg aggccctggc tatcgaagcc
      301 gttctggaag cgttgctcct tgcccagcat attttggtac cgctggaaga tgtccttgta
      361 tgagcggccc caggcgtggt gtacgcccat agggttgttc gcggtgattg gtccgtcgat
      421 gaacgagaac cgctcttccg ccccgttgtt gcggtcgaga tactggcgga cgacctgctc
      481 cttgtcccac cattcgagga ggtctcgctc catctgagga aaatcgacct tcgatgggac
      541 gtcctgaaaa gtctttgctg tcttcgttgt ctcaaccatg gtgtcctggt tctcccggaa
      601 cgttccacat ctggaatacg tgcgatgcgc agtcaatcgg cgtcctaggt ggcactcctg
      661 accatcgact ggacgggtag ccctcacccc ggacggtgcg gtccaaattg gaaccgaggt
      721 acgaagcctg gtatcaggca cgaaaaaggc ccgacctcag ggttgaggac gagcagtgcc
      781 gcggtaccac ctcagttggc cattcagcag aatagcccgc ttgatccgtg atatcggacg
      841 gaacccgatc gcgtctacca gtccccgaag gacgttggcg cgacggctca ggaacgatgt
      901 cccagtgtgc acttgatgat ccggcttcca tcgtcccgga cttgctggtc ggcgcactgt
      961 gggcgcggtt tccttcagcg cctcatgctt cttcaattac tttccagtgt accaaaaatg
     1021 gtggtgattc ctgcttctac gctcccttga tcatccatcg cttggtgcgc ctgtagaggg
     1081 cggtgagccc gaccagccac gtagtgccaa gcaggtatga tgcggccaca tcggtgaacc
     1141 agtggtgacc aagatagatt cggctggggc cgacgagcgc cagcagggat gtaagcaggg
     1201 ctacgacgac cctgcggatg gcattggccc taacccatgt gtcggcaagg taggcaagaa
     1261 acccgtatac gcccatatag atgatgacgt gtccgcttgg aaagcttgaa ccgccgatgt
     1321 tcgcgacagc gaccttgatc cctgtacctt ctgaactcgg gcgcttgcgc cgcatgatcc
     1381 gcttgaacgt actcgagacg ccgcctgtac cccatgcggc cagctggaag atcgcttcga
     1441 gacgcattcc cctgagccac agcgcggcgg caataccggg gggcaggatc cggctctgcg
     1501 gtgggaagcc aggccaggaa acgatgtgca tgagccgatc gaagtagggg tgatcctgtt
     1561 tctgcacccg cagggtcact gccgcgtcgg ctgcagcgct gcgattcctg cgaacgagcc
     1621 aaaagatgac agagaagagg accatggagg tcagcgatgc aatgaagcct ctcttgctac
     1681 gccgacgggc gacccgttcc ggattgagcg agatacttct cggcgtattg agaaaatgcc
     1741 gcagacccat atatccagat cccttccgtc cgtatcgttg gagcgcatgg tcgacccgtc
     1801 aagcggtaga ctttgagata gtaaccactc ctggtggatc tcgtctgtcg gaggcactgt
     1861 gtcgaccgtt ctcgttcgct caatctatcg ttactggctt tggtacgggg tcgtcctcgc
     1921 cggaatggcc gtcctgatgc tcctgccggg aggattcgcg gaaaaatcgc gcatgctgtt
     1981 gcatggcctc tgcgcgcagg ctcccaccca ttccttcacc ctcgggggcg tgctcttgcc
     2041 gttcgattcc cgcatgacgg gcatctatgg tggcaccctt gcaacgatga tctaccttac
     2101 agctcgatct cgattcttcg cctgcggtgt tccgccgacg aacgttgttg tcgtgcttgc
     2161 gcttttcgtg atcgccatgg ccgccgacgg gttcaactct ctcttcaccg atttgggcat
     2221 ctggcatccg tggactccgt ccaatgaatt gcgtctcatc acgggatatc tcgctggtat
     2281 aacgctcggc gcgatgcttt catggctgct cggctcggcg ctctaccgtg ttggtcagcg
     2341 acaggcagga atccaacgcc ccattgatgt tttagtcatc ctggtgccgc tttttccgtt
     2401 tggggcaatt cttctctctg gtgctgaatg gctgttcgtg ccgaccgcca cgctgctgat
     2461 cgtctctgcg tggacgacga tgtccgtgtt ggggttgtcg atcatcgtgc tcgcgttcgg
     2521 gctggatacc cgcatcaacc gccttcggga ccttcatgtc cccggagcgg ctgcggtgct
     2581 cctggggctg gcgatcatgc tcggacttgc cttcggccgg atgtggctgg agcacttcat
     2641 gggtatgcca tccactctgt aacgcggtgg cactttggta cactccactg atgctgggtg
     2701 cccggcaacg caccgcgctc accggtggtt accggccgtg ggactccggc gcggccggcc
     2761 gatctcgccc cgaattctcg tgatcggcac tgatcacgct ccttaatcgt cgtcattgct
     2821 catcgttcgc cccgggtggg gcggtcagga aacgttttct acctatgcgc attgttcgac
     2881 ataacgccca catctcgcag agaaagagac gtgcgcgcat cttctcgttt ttcggattct
     2941 tgctcttgtc gggatcgttg ctgatcgcct ggtatccgga atttcttctc ttcgcgtatg
     3001 ttgccatgtt aggcgggttc atcctcttca acatgggcat gcagcaacta ggcaagtgga
     3061 cgcgaaaccc tcgtaacgac cagttcctcg accaccagat gaagggcctg agcgacaaaa
     3121 tcacgctgat tcactatgcc caattcggta agcacaacat cgagcacgtt gtggtgcatc
     3181 ccgggggact ggtcgtgctg acggcccgtg aggtggatgg caccatcgag aagcgcggca
     3241 caaggtggag acgaaagggt ggattgtatc gtcgtctctt ctccttcagc gggccgcaga
     3301 tcggtaaccc gagtttcgaa acagatgcga atgtgacgag agcagaggat ttcctcgccg
     3361 agcaccagat ggaagtcgac gtcgagggcg ccatcgtctt tgtccacccc cgaaccgagc
     3421 ttgatatcga ggatccggat tacccagtgc tgcacgggga cgaggtggac gagttcatca
     3481 gggatctccc ggccgatgag tcattcactc gagaggagcg cgatcgcatc gtggaattgc
     3541 tgtccgccgg tgaacgcgtt gaaacgcctc aaacccagaa gcagggtgcc cggcggcccc
     3601 gaccggtcag gcgtgttgcg gcacccaaag tgaaaccgca gtagagaacg gacggaaggg
     3661 catgacggca accgggatag aggcaccgcc atcatcaaca ggctggcgag tctggggtca
     3721 cgatcacgcg gttcacgaac tacggtccgc catcgataag ggcgtccgcc acgcgtatat
     3781 tctcagcggg taccatcacg tggggaaaac cgcgcttgct caggagttcg cccgagcgct
     3841 gatgtgcggc tcctcaccgt ccccgggtca gccgtgtggc gtctgttccg catgccgaag
     3901 ggtggggcga ggagcgcatc ccgatgtgac actctatgac cttgcgaggc agcaggagac
     3961 ctctgacaag gcgtcgacca gcaagaatct cagcctgaat atccagaccg tgcgcgacat
     4021 tacctcaagc atctccttgc ggccgatgga agcccggcat cgggttgtca tcgtggacga
     4081 cgtcgaaacg atgcaggaaa cggctcagga agcctttttg aagacacttg aggagccgcc
     4141 gccgtacgca gtgatcctgc tccttacgac ggacgccgaa ttactcctgg aaactatccg
     4201 gagtcgctgc atgacgcttc atctgcagac agtccgcccg tcgacaattc tggatttgct
     4261 cctcgcgacc ggactggatg agaaggacgc gacggcgata gcggccgcaa gtattggccg
     4321 gcccggctgg gcggtccagg cggcgagcga tccgaacatc ctcagggaac ggctggaact
     4381 ggaatcgctc gtaaagggct ggatcaccgg cgatcaatac cggcggatgg tagaagcaac
     4441 gcggttgggt gacgtctttt ccaaggaccg tgccgcggtc tatgctcgtc tatccctcgc
     4501 ccagactatg tggagggaga tcgttctcca tgctctcggt gtggtcgatg tcgatcgccc
     4561 tgacaagaat atttttgagg gccagcagcc gaccgcgggg gagggtgtga cggctttacg
     4621 aagcgtggag cgatgcctta acgatctgga tgcgaacgtg cgtccaaagc tggcgctgca
     4681 atcgatggta ctccaatggc caacgatcgc cgcccgatga tctctcccaa gggaccaaag
     4741 gctggacccc caggtccagg acgcaccgag ctccctgacg catctgagga ccagaacggt
     4801 ttcaatattg tcggagttcg gctcttcgat tcaccccgtg tcgtttactt cctcctcagt
     4861 cgtggctcgg tccagccagg aacctgggtt gtggtgcggg cgcgccatgg cgaagaggca
     4921 ggacgcgtgg tgatcgcgcc acagcagata gtacttgcac aaatctcggg ggaaatagag
     4981 cccatcgaac gggtaatgag cgatgaagag attctccaaa tggaacgatt cctcaatgag
     5041 agcccggctc tcgttgagcg cgcaagggag atcgtccgcc gctgctggcc gcatgtcgag
     5101 ctgcgttccg ccaggtactc cttcgatggc tcgtatgtcg tgatatccct tgccgggtcg
     5161 gatggggacg agattctagg gttggaagaa gcgctggcag aggagttccg cgtgcctgtc
     5221 cggatccgta atgctggaac ccgacaaccg actcgtctga tgggcgggct ggggacattc
     5281 ggccggaaac ccgacactct ctctcgcgag aacgagcact acaggaagat caaggaggat
     5341 cttccacgat tgggccagcg ggtgagaaca cacgacggtg agggaatggt ggttgccttg
     5401 caggtcttcc gcgagctcgt cactgtccgg tatacagatg ggggacgcga aatggcgcat
     5461 ccagtgacgg acctgctccc cgagcgcgcc tgatccatca ggccgaacat cggttgggat
     5521 ggttgccagg gggtgcagtg cgcgacgtca agggcataac acggttagga caatgaacct
     5581 ggtgcccgca caagccggat ggagtcgctg cgtgtgaccc gtcgtttcag cggggtcggt
     5641 tcagtttgga aagttggagt ttcaatgcca ctctatctgg tcgttcatac gcctcggaaa
     5701 gacgacgagg agaagctgtt tccaccgacg aagatgctcg acatggctcg cgaccacgga
     5761 gccgaggaca tccgtacgag atggctcaag acctggtcgc cggacctcca tgatgagcgg
     5821 cacttcaccc tgtggagcgc aaactctgcc gccgatatca cagaggtcat gagccggtat
     5881 aacttcctga gcgagaccga cagtgaaccg atctgcgtca cggaatggtc gccagcagat
     5941 gtgctcgaat ccgctgacgc tgaggagtaa atccgccgaa agacaactta agatctcaca
     6001 cgatcacgtg ttgttgatag gtgtctttcc ttgcggaacc gaatatgcga atctgacgcc
     6061 agcacccctt cgggcaccct ggagcacgtg acctccacgg tggtccgagc cgtcgagttt
     6121 ggaacgggaa tcatcagatc aaggagcgcg tcgaatggca accgcctaca agactattgg
     6181 ccagcgcaca cggcgcctgg attcgccacc caagttgaca ggaaccgaaa agttcaccgc
     6241 tgatatgtcc cttccaggca tgctgcacgc acggatcgtg ggtagtgatc tcgcccatgg
     6301 ccggattctc agtatcgaca agagcgcggc gcttgatatg cccggcgtcg tcgctgtgct
     6361 aacgaatgcc gatctcccca tccaccggga tgagaacgac atcccggccg cggagctgtt
     6421 ggcttcagag gaggctttgc acgtcgggca cccgattgcg ctcgtgctgg cggagacaga
     6481 ggctgcagca caggatgccg ctgaactggt cgtggtggac atggaaccca tgccggtggt
     6541 gagtgacctc cacgaagcga tccgcgagga tgcgccaaga gtcagcgcgg acaaggctgg
     6601 cgcctttgag gacgaggcgg ccatgcacaa tgccgatgcc gcgggttcag ccgagaagca
     6661 ggaagacttg ccgcccaatg tttccaactc ggccaacttc gagcgcggcg acatcgatgc
     6721 cggattcgct gaagcggatg aaatcgtcga actctcgctg acatcggcgg cgatccatca
     6781 aggctatatc gaacctcagg tcgcgcttgc cgcgccggat gccttcgaca aactcaccat
     6841 ctacaccagt acccagggcg cgttccacgg gcggcagaag gtgtcggaca cactgggcat
     6901 gccgctggac tctgtcaata tcgtgccgat gccagttggt ggagggttcg gcggcaagtt
     6961 cgtcctcatc gagccgttgg tcgccgccgc cgccaaggca gtgaataggc cggtattgct
     7021 ccagtacacg cggtcggacg atttcctcgc tggcaacccg gcacctgagt gcgcgatcaa
     7081 gatcaagctc ggcgcgaagc gcgacgggac cttgacggca cttgatgtcg acctgctctt
     7141 cgatgctggc gcggcgtccg gatcgccgct cggtatcgct gccattctcg tcggcgggta
     7201 ctatcgcttc cccaaccttc gcatcaaggg atatgagacc aagacccacc gccccagctc
     7261 cgggtcctat cgggctcctg gggcacagca ggcaacattc gcgattgagt ccgcaatggg
     7321 cgagttgtgt gaccggctgg ggatggacca gatcgagatc cgtttgttga actgcgccga
     7381 agaaggcgat ctgcgcccga acggcggaga atggccgcgg atcggtttac gccagacgct
     7441 tgaggccctg cgggaccatc ccgcctggca gcagcgggac gcggccaagg cacaagggca
     7501 tggcatcggt gtcgcggtcg gtggatggcc cggcggaatt gagccggctt ccgcggcctg
     7561 ccgtctcgac ggggacggaa agctgacagt catgctcggg tcggtggacc tcaatgggac
     7621 gaacaccgcg ttcgcccaga ttgccgctga aggcctgggc acaggcgtcg aagatgtacg
     7681 ggtgcaaacg gttgatacgg ccggcgcgcc gttcgcgggc ggcaccggcg gctccaagat
     7741 cacctatacg gtcggcccgg cggtccaaaa ggccgtggag gatgccgtcg agcaaatcaa
     7801 gcaaattgcg gcggatcacc ttgaggcgtc gcccgaggac ctggagcttg tcgacggtgg
     7861 tgttcgggtc aagggcgtgc ctggttcctc tgtcacactc aaggaaatcg cgaggatgag
     7921 catgagcacc ggtaaaggac atatgccggt gcaggggcgt ggcggatcgg gcatcgcaac
     7981 gcaggcgccc ggatttgccg cgcacctggc tgaggtctcc gtggacgatc tgaccggcgt
     8041 cgtgaccatt caccggtacg tcgccgcaca ggatgtcggc tttgctatca atcccgccac
     8101 agtggaaggc cagattgaag gtggggtgtt gcagggcatc ggctgggcgc tatacgaagg
     8161 aatgccatac gacgaggatg gacagccggg ggcgtcgacg ttgatggaac acacgctgcc
     8221 ccgagcccga atggcgccgc ccatcgagac gctgcttgtc gaggtagcgt cagaggctgg
     8281 tccgtacggc gccaagggcg tgggcgagcc accagcaatc ccaggagcgg cggccattgc
     8341 gaacggaatc aaggatgcaa caggcgtgcg gatgacggcc ctgccaattc tacctgccgc
     8401 tgtactggag caacttggct aggaacggaa acaagaaacg gggctacctg caggttcagg
     8461 tggccccgtt tttcgtgcgc ggatcatcat tgcgcgggca tacttggtgc aaggaggatg
     8521 tcgatgctgg ttgcaggtac agcggcacgt cagcacaggc aggagccata tgctcgtaga
     8581 actagccatc agcgatctcg caatcatcga gcgtaccacc atccattttg aggagggact
     8641 gaacgcactt actggcgaaa caggggcggg gaagtcgatt ttgctcgacg cactcggagc
     8701 ggttctcggg cagcgggtga gcagcgacct cgtgcgcacc ggcgccaagg cggccagggt
     8761 cgaggctgcc ttcgatctct ccggtgaaga aggcccacgc gtcgttgaga tcctgggatc
     8821 ctacggaatc gagcagcgac tggatgagcc tctcctgctc agccgcgaaa tcagttcggc
     8881 tggaagatcc tcggctcgca tcaacggtcg tatgtcgacc gttgcgcagc tcaacgagat
     8941 cggcgcgatc ctggtcgaca tccacgggca gtcggatcat ctctccatcc tccaaacgtc
     9001 ggagcaacga agcatcctgg atcgatacgc ccggctggag ctgctccgcg atgaggtggg
     9061 cgtccttgtg cgggagtatc tagaggtcag gcgtcttctc gaagacgttt cacgagggtc
     9121 acgtgagcgc gagcagcggc tggatctgct gaggtttcag ctaggggaaa tcgatgaggc
     9181 agggctggcc tctggcgagg atgagcggct cattcgggag agggaggtcc tgcaaaacgc
     9241 cgatcgactt cggcagtcgg caatgtcggc tgtcgaagca ctggtcggcg acgaatccgg
     9301 cgcggttgag gtcagcgcga acgcgctcct ccgtcaagcc gagcatgccg cggccgatct
     9361 gggcgagatc gatgaaactg cgcaagagct gtcggagcga gcgacggagc tcgtcgtgct
     9421 ggcagaggac ctggtccgag acctcaggtc gtacgcggac acgatcgagg gtgatcctga
     9481 gcgacttatg gaagtcgagg atcgactgga tctgatccag accctgaagc ggaagtatgg
     9541 tgggtcgatc gacgaaatcc tggcattccg tgatgaggcg gaccgggagc tgcagcagct
     9601 gacgggcacc ggttttgacc gggaagcgct tgtggcgcga gaggcatcga tatcccgcga
     9661 gcttgcgtct tgtgccagct tgctctcgga gaaacggagc gaggtttcct tggaactgag
     9721 cgcccgtatt gtcgagagca tatccgagct ccgaatggga agctccaatg tctccgttga
     9781 ggtgcgtcaa cgggaggatg cggacgggat cgagctgcca gacggccgca gggttcacgt
     9841 ggacgaaaca ggcatcgacg aagtggagtt tatgattgct ccgaacgctg gtgaaacttt
     9901 gaagccgttg ggcaggatcg cgtcgggcgg tgagactgca cgggtcatgc tagctgtgaa
     9961 atccattctg agtgctgtcg atgagacccc gacactcgtc ttcgacgaga ttgatgttgg
    10021 agttggaggg cgttccggcc agatggtcgg tgaaaagctc tggtcgttga ccgaccggca
    10081 tcaggttatc gtcatttcac accttgccca gatagctgcc ttcgcggaca agcactttcg
    10141 gatcgttaag gaaccaagag agggccgggt ggtgtcctct gtccaagagc tggatcggtt
    10201 gacacgggaa gaggagctgg cggcaatgct tgacggtgtg cccgccactc ccgagtcgct
    10261 tgcgaacgcc cgagcgatgc ttcagcgtgt ctatgattac cgttcccgtg gcatgcgcaa
    10321 gcaggaagcc atttcctgag cggctatact tttgccagcg tggtgcttcc gggccgtggc
    10381 cttaatatcc tgaaggagaa caatcaatgc agccaaacat gaagatgatt cagcagatgc
    10441 agaatcggat gatgaagatt cagcaggagc tggaagaaac tgtcgtcgag ggtaccgctg
    10501 gcggaggcgt cgtcaaggta gaggtcaacg gccagcgggc gatgcagtcg atcaagatcg
    10561 atccgtcagc ggtggatccg gaagatgtcg agatgcttga ggacctcgtt gttgccgcag
    10621 tgcaggacgc aatggacagg gcttccaagc tggcagagga caagatgggc gcacttacag
    10681 gcggcatgaa gattcccggc ctgatgtaga gccttgggcg tcttctgatg atccttagcg
    10741 aaccgattcc attctatatc gacgatatcg tccgccttcg taaaccccat ccgtgtggct
    10801 cgtacgagtg gaaggtcgtg cggattgggg cagacatcgg gctcgtctgc caggaatgtg
    10861 gcaggcgagt gctcttgccg cgtcgtgtgc tcgagaagcg cttgaaggcc ttcgtgtcac
    10921 gagggcccac cgctcctggt gcccctgccg cagatgacca aataacgtcg ccatgagcgc
    10981 cgcaagcgtg cgcgacaaga atgggaaacg ggacgttcgt cccgactcga tgggcgccgt
    11041 cctgcggaac ggcgcgtttc tgcgtctctg gctgatccag gcgaccaccc aaatcttgca
    11101 gaatatgatc aacttcgcct tgctcctccg ggttcgcggg gttgttgaag tgcatgacct
    11161 tccccatgcc aacacagcga tcagccttgt cattcttgcc ttttccctgc ccgccgtgct
    11221 gttcggtccg gtggccggtg tcatcgctga ccgtgtaaac aagcgcaacc tgatggcagt
    11281 gctgaatgcg gcccgagccg tagcagtgct tctcttcctc gtcattcggc cgggatggga
    11341 tgtcgaaacc atcctcgcgg cgtactacat cgtgacattc cttttcggca tcgccgggca
    11401 gttcttcgca ccggtgcttg gtgcaacgat tccgctggtg gcgaagcggc agcaactgat
    11461 gtctgcgaat gcgctcttca atctgacatc gacggcggct cagctcatcg ggtttgcggc
    11521 agccgggcca atcctgatcc gggtgcttgg cgtcgatcgg ctgttcgccg ttactgtcgt
    11581 cgcctatctc atcagtgtgg cgttggtctt tacgctgccg aagacgccag ttccggtgcg
    11641 cgcgatcaac gagatcgagc tgcggccaat gcagcggctg tggagcgaca tgaaggaagg
    11701 cctcgtcttt atccttcagg atccggcgct catgcgagcg attggttatc ttacgctcgc
    11761 ttcaacgaca ttcctgatga tcgcgacgct cggccctgac tttgtttcga gtgtgattgg
    11821 attgccgaag gaagacatcg ggttcatcgt cgcgcccgcc ggtgcgggca ttttggccgg
    11881 ggtattgatc gtgccgcgga tcgtgaggag aatcggtcgc gactggctta tcgatatctc
    11941 gctgaccatc gccgggctca tgctcgcgct gatggcgctc acgaggggcg tgctgaatgg
    12001 gatagttccc ggtaatgcgc cgttgtggac ggtcgtgggc ttcaccgcca gcttcgcggg
    12061 gatcctgggt ctctgcaacg cattcattct tgtgccgtcc caaacgatgc tgcaggaacg
    12121 gtcgcatgaa ggagtgcgcg cgagggtcta cgcgacgttc ttcactgttt caaacaccgt
    12181 ggcctttgtt ccaatcttct ttgccgccgc cgcggcagac atttttggcg tcatcaatgt
    12241 gcttatcgtt atggcctttt ttgtcggtgg gctagggttc tttggacttt tccacaggag
    12301 gcgggcagag cgcaacaagt gggagcgcgt gaggacccgg caccgacagg gtcccgatgc
    12361 catcccgaga agattcgagg gatctggaaa gcccgtcaag tcataagtgt caatgccaga
    12421 atatcggtcc tcaatgcata acggaatctc tccacttacg ccaacaactg atgcagaagc
    12481 gccatgcgga tgaagacccc gttccgggct tgccgaaagt aggcagcacg cgggtctgaa
    12541 tcgacctcgg ggtgaatctc atcgacgcga ggcaggggat gcatcacgat ggcgtgttcg
    12601 ccaagatctt tcatgttatc aggagtcatg atgtagatgc cttgtgcctc ggtgtattcc
    12661 tctggagtag cgaaccgttc ccgctggatt cgggtctggt acacgacgtc gacctcgccc
    12721 atagccttcg tgagatcggg ctcctcccgc catgggactg agtcatgatc aagagcgtcc
    12781 ttgatgtcgt ctcccatcgg tacggcgccg ggggcaacga agaccagctc ggtgttacgg
    12841 gtcttgcgaa acagcagggc gagcgatcgg gccgcgcggc cgaatcgaag atcgcctacc
    12901 agggcgacct tgaggtcgtc aatttgacca agctcgttct ggatcgtgta gaggtcgagc
    12961 agggcttgtg tggggtgttc tccaggtccg tcgccaccgt tgatgattgg cacaggggag
    13021 gcattggcgg cgcgctgggc agcacctcgc tcgaagtggc gaatgacgat gcaatcggcg
    13081 taaccggcga caatgcggat tgtgtcctcg acggtttctc ccttgatggc ggaggagaat
    13141 tcgcgggcgt tttcggtgga gatcacctga ccgtccagcc gcagcattgc agattcgaaa
    13201 ctgagccggg tcctggtgga gggctcatag aagagcgtgg cgaggatttt cccccgcagc
    13261 aagccttcag ccgcggtaac gctccgcatt cgatcggcgt ctgcgaacag cgtctcaatc
    13321 catgctcgtt cgaactgctc aaccgcgacg atatgcttca caggtgctcc ttccaagagg
    13381 gtctgaaggg aatgccgacg ggtgagagta tagttccggg gacgaccata tcaggattgc
    13441 gggagcgaga gcatgttgca gcagttgact gagcgcgtct ttgtcgccgc agggggcacc
    13501 aacaccggag tcgtcatctc tgatgatggt cgagtggtat tgatagatac ggggttgaac
    13561 gacacccatg cgaggaaggt cttgcgggcc gtccgctatg atctagggac agaggttcgt
    13621 gcgattctta ccacgcacgg tcatgcggac cacttcgggg cgaacgcctt tgtcgtcaag
    13681 cgcacaagtg catcggttca cgctccggac gttgacgaga tggtgctccg gcaccctttg
    13741 atgcagaccg tgatgctctt cggaggtgcg gatccagtgg ataccctgcg gacccgattc
    13801 ctgctcgccg aggacggacc cgtggacggg ctccttgaac ctggaaacca ggtactggag
    13861 ggggtggaac tggatatcgt ctctcttgcc gggcattcca tgaaccagat gggcattgtc
    13921 atcgacagta tctttttctg tgcagatgtc gtctttcctg attccacgct cgagaagtac
    13981 cggattccct atctgtacgg gctcacggaa catctggacg cgcttgattt ctccctctcg
    14041 gtggcgtgcc ggcaagttgt gccgggtcac ggtccggtgg aggaggggat tacaggtccg
    14101 gtcgagcgga atcgcgaggt gatcaaccgc accatcacgg ccctgctctc agttctcgat
    14161 acaccgaagt caaccgatca gatatgcgtg gacctcttca ccgcactcga tgtgccagtc
    14221 ggggatgacg gcgggtactt cctgctgcgt cccaccgtcg cggcgtatct atcccatttg
    14281 cagcgggtca accaggtgtg caccgaggtc gcgaacaagg gggtcgtgtg gcggcgcaca
    14341 tgagcagacg aagagttttg taagtcctgg ggccgatctc attgacctgc cgacgatagc
    14401 catggcgtgg tttgatcata tgccagggac gattggaaat cggtgccgag tgtcccaagc
    14461 ccatttccag gttacacgct cgaagcggtc ggggtgcgcg gcccgagcac tattcctgaa
    14521 cgatcggttc ggcgccccgg cattttatgt cgtcgaggat caactttgcg atgtcctcgc
    14581 cgagtcgcac gctgtaattc gttcccctgt cttccggata gatttgtgtc gtattcgcga
    14641 gatagaggtt tttcagcggc gtccggtgct ccgggatcat ctccgagtag ttcagcggga
    14701 tgatcggctg agccgcacgc tcccggaaga cccaggtctg cttgacccac gttggatcga
    14761 agttcgggtt gatcttctcc ag
//
LOCUS       DASYWM010000031         7320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_526616, whole genome shotgun sequence.
ACCESSION   DASYWM010000031 DASYWM010000000
VERSION     DASYWM010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526616"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VGR29_01270"
     CDS             <1..184
                     /locus_tag="VGR29_01270"
                     /inference="COORDINATES: protein motif:HMM:NF025445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4260 family protein"
                     /protein_id="HEV2072245.1"
                     /translation="YNAVHSSVLPVILALVGVMTDGSLLTAIALIWLAHIGFDRMVGY
                     GLKYAAGFKETHLARI"
     gene            complement(273..3215)
                     /locus_tag="VGR29_01275"
     CDS             complement(273..3215)
                     /locus_tag="VGR29_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV2072246.1"
                     /translation="MKKSFEFTATDVVSTVASDEELRARIEQLELVLRATEEGLWDWN
                     IVTDEVYFSPRWKAMLGYADEEFRNSTDEWQQRVHPDDVDSAVGAIRGYVAGERDNCE
                     NTHRLRHKDGSYRWMYTRGILTRDSEGRPVRLVGSHADITIRKRAEEDLKRRDAILEA
                     VRFAAERFLRAGTSWEESIQDVLERLARATQVSRTYVFENYVGDDGELRNKITHEWVA
                     PGVVPRIDNPTYRKVSLKNAGLARWRDILERGEIIFSHVRDLPDSERSRAEGEGAVSF
                     AIVPIFVEGAWWGVIGFDESAHERDFSVPEQDALKAAADLMGAAIGRKRVEERSARLA
                     AIVESTHDAIVGMLRNGTITSWNRGAEDLYGYTAEEMIGHSIRPLMPLEELPRLRDVL
                     IRLTAGQRLDHFETEFIRKDKTHVAVSMSFSLVRDRDGEVTGASAIARDISEQKRAEA
                     ELRESQRSLSTLLSNLPGMAYRCLNQPDWPMDFISEGSLDLTGYSAAELVANTHVSFA
                     DLIHPDDRDQVWRGVQEAIALDQPFRMNYRIATADGAEKWVMEQGRGVFDEWNQIQAI
                     EGIIIDVTDRVQARRELEQRVEERTRELATVLNVSSSIASTLELETLLGVILEQFEQV
                     VPHTAASISFLENEMTLRLLDYRGPMDQADLVWVWPSHENEHSWEVIRSGKPVIIPDV
                     RAETPLAQAFQRKAVYDTGGVPIYISSWMGVPLIFRDKVIGLLAVDSSDVDAYTSRDA
                     ELALAFATHAAVAIENARLYEQAQGKAALEERHRLARELHDSVSQALFGIGLGARTAR
                     TLLDSDPGKAVAPIEYVLSLAEAGLTEMRALIFELRPEALEQEGLVAALEKQVATLRT
                     RHGLEVVAEFEHSAGVENYVEEAVYRIAQEALNNITKHAQASKVDVRLAHERGRIVLS
                     IADNGNGFDPSALYSGHLGLKSMRERAERLGGSLQIESAPGAGSRLEAAIPASPGSNA
                     TPGLRRAV"
     gene            3639..4070
                     /locus_tag="VGR29_01280"
     CDS             3639..4070
                     /locus_tag="VGR29_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2072247.1"
                     /translation="MTTSMGMTLRLELFVHNMDVSIDFYCRVFGFELVRKESDYASLR
                     SGDVILGLGPISKLPKEDGYFTRARLASDRGAGVEIVLEVDDVLALHERVGASGYPVL
                     HTLQRRPWGLTDFRITDPDGYYLRITSRNQEANPDERVLQE"
     gene            4096..5820
                     /locus_tag="VGR29_01285"
     CDS             4096..5820
                     /locus_tag="VGR29_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2072248.1"
                     /translation="MSTQERPDQQMSRPAILVVDSDHAELERTVHALARRFGADYFVV
                     SAPTAPQGLQVLADLASKEAEVALVAAALRMTGGDGPAFLDEVRSRYPGAGRALMIEM
                     GDTTATESVRYATARSQIDFCIVGGWESPEEWLYPQVQEALTKWANAHRPRHEHVRLI
                     GEQWSPRSHELRDLLTRNMVPFGFYDASTEVAQRLLAEHGLSPDALPTIILFNGEVIA
                     EPTNMDLATILGVRTQPDSGLYDLVVLGAGPAGLAAAVYAASEGLRTVVIEPQAIGGQ
                     AGSSSMIRNYPGFPRGISGGELTLRAYEQAALLGAQFVFASRATGLSTRGNERIVMLS
                     DGSEVACRSVLIATGVSYRRLNIPSVDRLLGMGVFYGAAAAEAGAMTDQHVCVVGGAN
                     SAGQAALHLAKVAARVTILVRGASLASGMSDYLVREIDATLNIEVRTQTRVVDGGGNE
                     RLESVVLEDLRTGTRNEIAAAGVFIMIGAEPRTDWLEGAVERDPYGYVPTGRDVSLDH
                     WPLERPPMLLETSVPGVFAIGDVRSGSVKRVVSAVGDGGVAVGLIHQYLAQAQEQSLV
                     TQQLVTAQ"
     gene            complement(5867..6508)
                     /locus_tag="VGR29_01290"
     CDS             complement(5867..6508)
                     /locus_tag="VGR29_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2072249.1"
                     /translation="MTIRILIADDHSVVRQGLRMFLELDDELEIVGEATNGAEAVARA
                     KELHPDVVLMDLLMPVMDGLTAISTMRREMPGVEVVALTSVLEDASVVGAIRAGAIGY
                     LLKDTNAEELRRSVKAAAAGQVQLAPEAATRLMREVRAPEAPEALTDRETDVLRLLAD
                     GQSNKEIARNLTIGEKTVKTHVSSILSKLGVQSRTQAVLQAMRDGLIPPAEST"
     gene            complement(6666..>7320)
                     /locus_tag="VGR29_01295"
     CDS             complement(6666..>7320)
                     /locus_tag="VGR29_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV2072250.1"
                     /translation="AAGACRMLSIPRDTRVEIPGIGYTKINHALMEGGVPLATETVER
                     FLGIDIDHYGLIDFVAAAQVIDALGGVTVTNEYAFDIGGNHYPAGEQRLDGARAALYA
                     RYRYGPDGDFGRIHRQQQVFAGLLRELQGASVTQLTQTAWGLLGPHMMSDLTPAMLLE
                     LASTDGGSCNGEALVIDTIPDTAQGYYWDDLFQQELWFVVVDPAVVQQKVDALIHGE"
BASE COUNT         1428 a         2319 c         2132 g         1441 t
ORIGIN      
        1 gtacaatgcg gtgcattcat cggtcctgcc cgtgatcctg gccctagtcg gcgtaatgac
       61 ggatggttcg ctcctcaccg ccatcgcgct catttggctc gcccacatcg gcttcgaccg
      121 gatggtgggc tatggactca aatatgcggc aggattcaag gagactcatc tcgcacgcat
      181 ctgaccgggc gcggtaacgg cgactgactc cgatatcctc cgccgaagcc cgtgcgcgga
      241 agaggtgaag cggagcacgg gaggacgcaa gctcacactg cccggcgcaa tcccggcgta
      301 gcgttcgaac caggcgacgc cgggattgcc gcctcgaggc gagagccggc accaggagcg
      361 ctctcgatct gcagcgagcc tcccagtcgc tcggctcgct cgcgcatgct cttcaaccca
      421 aggtggcccg agtacaaggc cgatggatcg aaaccgttcc cgttgtcggc aatgctgagg
      481 acgatccgtc ctctctcgtg agcaagacgc acgtcgacct tcgatgcctg cgcgtgcttg
      541 gtgatgttat taagggcttc ctgggcgatc cggtacacgg cttcctcgac gtagttctcg
      601 acaccagcac tatgctcgaa ctccgcgaca acctccagcc cgtggcgggt tcgcagcgtc
      661 gccacttgct tctcgagggc agcaaccagc ccctcttgct ccagcgcctc tggtctcagc
      721 tcgaagatga gcgcccgcat ctcggtcaac ccggcctcag ccagcgaaag gacatactct
      781 attggcgcga cggccttgcc gggatcgcta tcaagcaacg tgcgcgcggt gcgggcaccg
      841 agcccgatac caaagagggc ctgggaaacc gagtcgtgca gctcccgcgc caaccgatgt
      901 cgctcttcaa gcgcggcctt gccctgggcc tgctcataca gccgcgcgtt ctcaatagcc
      961 acagcggcat gggtggcaaa ggcaagcgcg agctcagcat cccgtgacgt ataggcgtca
     1021 acgtcactgc tgtcgacagc gagcaatcca atgaccttgt ccctgaagat gagtggcacg
     1081 cccatccagg aggagatgta gattggcacc cctcctgtat cgtagacagc cttcctttgg
     1141 aatgcctggg cgagcggcgt ctccgctcgc acatcgggaa tgatgaccgg tttcccgctc
     1201 cgtatcactt cccaactgtg ctcattctcg tgggacggcc aaacccagac gaggtccgcc
     1261 tggtccatcg gaccccggta atccagcagg cgtagcgtca tctcgttctc caggaaggag
     1321 atcgacgccg cagtatgggg cacaacctgc tcgaactgct ccagaatcac gccaagcaac
     1381 gtctcaagct ccagcgtgga cgcgatgctt gaggagacgt tgagcaccgt tgccagctcg
     1441 cgcgtccgct cctcgacacg ttgctccaat tcccggcgcg cctgcacgcg gtcagttaca
     1501 tcgataatga tgccttcaat cgcctggatc tggttccatt cgtcgaacac gccacggccc
     1561 tgctccatga cccacttctc cgccccgtcg gctgtcgcga tcctgtagtt catccgaaag
     1621 ggttggtcga gggctatggc ctcctggaca ccacgccaca cttgatccct gtcgtcggga
     1681 tgaatcagat cggcgaagga gacatgcgtg ttcgcgacca actcggcggc actgtagccg
     1741 gtgagatcca gcgacccctc gctgatgaaa tccattggcc aatcaggctg gttcaagcag
     1801 cgatacgcca ttccggggag attgctcagc agggtgctaa gtgaccgttg gctctcccgc
     1861 aactccgcct cggcgcgctt ttgctccgag atgtcacggg cgatcgccga ggcccccgtt
     1921 acctccccat cccgatcacg cacaagcgag aaggacatgg acacagcaac atgcgtcttg
     1981 tcctttctga tgaactcggt ctcgaaatga tcaagccgct ggcctgccgt cagcctgatc
     2041 agaacatcac gaagcctagg aagctcttcc agaggcatga gtggacgaat tgagtggcca
     2101 atcatctcct cggccgtgta gccgtagagg tcttctgcac ctcggttcca ggacgttatg
     2161 gtgccattac gcagcatgcc aacgattgca tcatgtgtgg attcaacgat ggcggcaagc
     2221 cgggccgaac gctcctcgac tcgcttccgc ccgatggccg cacccatcag atccgctgcg
     2281 gccttgaggg catcttgttc aggaaccgag aaatcacgct catgtgcgct ttcgtcgaat
     2341 ccaatgaccc cccaccacgc gccttccacg aaaatcggga cgatcgcgaa ggacaccgca
     2401 ccttcgccct cggcccgcga tcgttcagaa tccggaagat cacgcacatg gctgaatatt
     2461 atctcgccgc gctcaaggat atctctccag cgagccaggc cagcattttt tagggaaacc
     2521 ttcctgtatg ttggattgtc gatcctgggc accactccag gtgcgaccca ctcgtgggtg
     2581 atcttattcc gcaattcacc atcgtcgccg acgtagttct cgaacacgta ggtgcggctt
     2641 acctgagtcg cgcgtgccaa tcgttccagg acatcctgga tgctctcctc ccagcttgtg
     2701 cctgccctga ggaaccgctc ggcagcgaat cgaacggctt ccaggatggc atcacggcgc
     2761 ttgagatctt cctcggcccg cttccggatg gtgatatcgg catgcgagcc aacgaggcgg
     2821 acgggccgac cttcgctatc gcgcgtgagg atgcctcggg tgtacatcca tcgatacgag
     2881 ccatccttgt gccggaggcg atgggtgttc tcgcagttat cccgctctcc agccacgtag
     2941 ccacggatag cgccgaccgc cgagtcgacg tcatcgggat gcacgcgttg ttgccactcg
     3001 tcggtgctat tacggaattc ctcgtcggca tagccgagca ttgccttcca acgaggcgag
     3061 aagtagacct cgtcggtgac aatgttccaa tcccacagcc cctcttcagt tgcccggaga
     3121 accaattcca gctgttcgat gcgggccctg agctcttcat ctgatgcgac ggtggagacc
     3181 acgtcggtgg ccgtgaactc gaagcttttc ttcatgaccg atcttcccgg caggaaaacg
     3241 taatggcttg tttagagatg aggagactgt ttgcacttct tccggagaac cgggtgcctc
     3301 ccgctagccc ggcccgagcg ggtggggact ttccttgagc aatccgacag cccgacatat
     3361 gagagttcta aagtcccgac cagccgtcgc gtcataaata gaagtccgat ccaggcgaat
     3421 gctccgcctt ttgacggacc tccatattcc gcccagatgc cgacccaaac ttccgccagt
     3481 cgccagaggt cctactgcct gaatcctctc aaactatatc agaggattgg acgcagacta
     3541 cgccagcagc acttgaggac aacatccgtg aacgtaagtt gctcaccccc tcacccaaca
     3601 tcttcagggc caaccgggag ggaggtaaga gggagatcat gacgacctct atggggatga
     3661 ctttgcggct cgagttgttt gtccacaaca tggatgtatc catcgacttc tattgtcgcg
     3721 tgttcggctt tgagttggtg aggaaggaat cggactatgc cagcctgcgc tccggcgacg
     3781 tgattcttgg gcttggacca atctcgaaac tcccgaagga agacgggtac ttcacccggg
     3841 ccaggttggc gagcgatcgc ggcgcaggcg tcgagatcgt gcttgaggtt gatgacgtgc
     3901 tggcgctcca cgagcgcgtc ggtgcctccg ggtatcccgt gcttcacacc ctgcagagac
     3961 gcccgtgggg attaaccgac tttcgaatta ccgaccctga cggctattac ctgcgcataa
     4021 cctcccgaaa ccaggaagca aaccctgacg agcgcgtgct tcaggaatga ttgtgggatt
     4081 cgtacggtga ggaagatgag tacacaagaa cgacctgacc agcagatgag ccgcccggca
     4141 attctcgtgg tagacagcga tcacgctgaa ctcgagagga ccgtgcacgc gctggcacgc
     4201 cgcttcggag ccgactactt cgttgtatca gcgcctaccg cacctcaggg acttcaggtc
     4261 ctggcagacc tggccagcaa ggaagcagaa gttgcactgg tggcggccgc attgagaatg
     4321 accgggggag atggtccagc gttcctcgac gaggtccgtt cacgctatcc cggcgctggc
     4381 cgggccctca tgatcgagat gggcgataca actgcgaccg agtcagtccg atacgcgacc
     4441 gctcgaagcc agatcgattt ctgtatcgtt ggcggatggg aatcacccga agagtggttg
     4501 tatccacagg tgcaagaggc gttgacgaag tgggccaacg cccaccggcc gcgacatgag
     4561 cacgttcggc taatcggcga acaatggtcg cctcgcagtc acgagctacg ggacctgctg
     4621 acgcggaaca tggtgccgtt cggattctat gatgcatcga cagaagtagc acagcgcctg
     4681 ctcgccgagc acggattatc ccccgacgcc ctgcccacca tcatcctctt caacggcgag
     4741 gtgattgctg agccaaccaa catggacctc gccacgattc tcggggtccg cacgcagcct
     4801 gactccggtc tctacgacct cgtggtcctg ggagccggcc cggcagggct tgcagccgct
     4861 gtctatgctg cgtcggaagg gctccgcaca gtcgtgatcg agccccaggc aatcggtgga
     4921 caagccgggt caagttcgat gatccggaac tatccgggtt tcccgcgcgg catcagcggg
     4981 ggcgagctga cgctgagggc ctacgaacaa gccgcgcttc ttggggcgca attcgtcttc
     5041 gcgagcaggg cgaccggact ctctacccgg gggaatgagc gcatcgtgat gctctcggac
     5101 ggcagtgagg tcgcctgtcg ctcagtgctc atcgccacgg gggtttccta ccggcgattg
     5161 aacatcccct ccgtcgatcg gctgctcggg atgggcgtct tttacggggc cgccgctgcg
     5221 gaagccggtg cgatgaccga ccagcacgtg tgtgtggttg gcggagcgaa ctccgcgggc
     5281 caggcggccc tgcacctcgc caaggtcgct gcccgcgtga ccatcctcgt ccggggtgca
     5341 tcgctcgctt ccggcatgtc tgactatctg gtcagggaaa tcgatgccac cctcaacatc
     5401 gaggtccgaa cgcagactcg cgtggttgat ggaggcggca acgaacgtct ggagtccgtt
     5461 gttctggaag atctgcggac cggaacgcga aacgaaatcg ccgcggcggg tgtattcatt
     5521 atgatcgggg ccgagccacg gactgactgg ctggaagggg ccgtcgagcg cgatccttac
     5581 gggtatgtcc ccacaggccg ggacgtttcc ctggaccatt ggccccttga acggccgccc
     5641 atgttgctgg agaccagtgt gccgggtgtc ttcgccatcg gcgatgtgcg tagcggctca
     5701 gtgaaacgtg tggtctccgc ggtgggagat ggaggagttg ctgttggact cattcaccaa
     5761 tacctcgccc aggctcagga gcaatccctg gtgacacagc aactcgtcac agcgcaatga
     5821 cggaatatcg gccgtgggcg ggctacctga ggatcgggag cgatcctcag gtactttccg
     5881 ccggtggaat gagaccatcc cgcatggctt gcaacactgc ctgcgtccgg ctctggacgc
     5941 cgagcttgga gagaatgctg ctgacgtggg tcttgacggt cttctcaccg atcgtgagat
     6001 tccgcgcgat ctccttgttc gactgcccgt cggcgagcag ccgcaagaca tcggtttcac
     6061 gatccgtgag cgcctccgga gcttccggcg cccgaacctc acgcatgagc cgtgtggcgg
     6121 cctcgggagc aagctgcacc tggcccgcag cggcggcctt gacagagcgc cgcaactcct
     6181 cggcgttggt gtctttcagc aggtatccga tcgcaccggc ccggatggcg ccgacaacgg
     6241 atgcatcctc cagaacgctg gtcagtgcca cgacctcgac ccccggcatc tcccggcgca
     6301 tcgttgagat cgccgtgaga ccgtccatga ccggcatcag aaggtccata agcacgacgt
     6361 cggggtgcag ctccttcgct cgcgcaaccg cctccgcccc gttggtcgct tcgccaacga
     6421 tctccagctc gtcgtccagc tccaggaaca tgcggagccc ctgacgaacc acactatgat
     6481 cgtcggcaat gagaatgcgg attgtcattg tgccctacta tcttccgacc accacccact
     6541 catcgcgtac tctccctggc cgcatccgcc gctgattcct cagctcttcg atgtcccgac
     6601 tccactatat acgggagacg gattaatcga aaaggtttca tgaacgcggt tgcgccgggg
     6661 ctctgctact ccccgtggat caaggcatcg accttttgct gcacgacggc aggatctact
     6721 accacaaacc agagctcctg ttggaaaagg tcatcccagt agtaaccctg tgcggtgtca
     6781 ggaatcgtgt cgataacgag agcctcgccg ttgcaggagc cgccatcggt tgaagcaagt
     6841 tccaggagca tcgccggtgt gaggtcgctc atcatgtgcg gtcccaacaa tccccatgcg
     6901 gtctgggtaa gctgcgtcac cgatgcaccc tgcaactcgc gaagcaggcc ggcaaagacc
     6961 tgctgctgtc gatgtatgcg cccaaagtca ccgtctggtc cgtagcggta ccgggcatag
     7021 agcgccgccc gggcgccatc caggcgctgc tccccagccg ggtaatgatt gccgccaata
     7081 tcgaaggcgt actcgttggt gactgtcaca ccgccgagag cgtcgatgac ctgggcggca
     7141 gccacaaaat cgatcagccc gtaatgatcg atatcgatcc caaggaatcg ctcgacagtc
     7201 tccgtagcca acggcacgcc gccctccatc aaggcatggt tgatcttggt gtagccaata
     7261 cccggaatct ccacgcgtgt atcccggggg atgctcagca tcctgcacgc gcctgccgcc
//
LOCUS       DASYWM010000032         8378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_113883, whole genome shotgun sequence.
ACCESSION   DASYWM010000032 DASYWM010000000
VERSION     DASYWM010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113883"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VGR29_01300"
     CDS             <1..443
                     /locus_tag="VGR29_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV2072251.1"
                     /translation="EIIPRVDVLFASRGALQTFYGLEGAYEDVLRQAIEKLGVAAVTV
                     NRKRAKGSRRLKLTSMAMGQSGVLASSEPRDIEVLDRLGGGDAFAAGFLASYLDNPHG
                     LTRAVSLGAAACALKHTMPGDFLCATKEEIEDAVKSVDAGVLQR"
     gene            complement(516..1460)
                     /gene="thrB"
                     /locus_tag="VGR29_01305"
     CDS             complement(516..1460)
                     /gene="thrB"
                     /locus_tag="VGR29_01305"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV2072252.1"
                     /translation="MQFSVRAPASSANLGPGFDTIGIALDIWNEVTIDTDGEPGSVIN
                     EGPESALLEGHENLTLTAMRMLAREYRRKLPAFSLTAYTGIPVSRGLGSSAAALVVGL
                     IAANRLLDLDLSQDDLFQMAWRIEGHGDNVGATMYGGAIMAVPHITGVTRLWHKEDLG
                     LTCVLFIPEATGATHAARAALPNTVPHTDARFNVATVAGLAMGLKLQDHALIAAGMHD
                     RLHEPYRARLFPHLNAMKEAAKRAGAIGAVLSGAGPTVLALATLEKAVEVHAAYEAAA
                     HLLGVPGQAHIIGPSSDGATANIPTPAGASNEPIFPGR"
     gene            complement(1444..2763)
                     /gene="thrC"
                     /locus_tag="VGR29_01310"
     CDS             complement(1444..2763)
                     /gene="thrC"
                     /locus_tag="VGR29_01310"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV2072253.1"
                     /translation="MTAPGESTSVSSSPGLSVTCMACGRTFDGYQSLTTCPTCGGLLD
                     AWIDVAQQLTPASFDAARGSFEALSGVWRFRPLLPPIADEHIVTRYEGNTPLYWDERL
                     GAYAGLSGGRFGVKHEGHNPTGSFKDRGMTVALSHAKATGARIVACASTGNTSASLAS
                     YAATAGIPAMVLIPDGKISASKLSQTIAYGATVVQVEGDFDVALQLLRQLTDAYDVYL
                     ANSVNPFRPEGQKTIVFEILDTLGWQVPDIIALPGGNLGNTAAFGKALSEAFQVGLID
                     TLPRIATIQAAGAAPFAAFYESGFDHYEPVQADTIATAIKIGAPASVDRARRTIELTS
                     GIVTSVTDEEILDAKATIDAAGIGCEPASAASLAGVRKLVTQGEIDPGATVVGVLTGN
                     VLKDTEAVVSYHLQDLEGEPRPRANRPVKVVAQLEALKRVLDHAVLG"
     gene            complement(2902..3114)
                     /locus_tag="VGR29_01315"
     CDS             complement(2902..3114)
                     /locus_tag="VGR29_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV2072254.1"
                     /translation="MHLDIGIERSRKNRWIRGVCAGIAYKFGVDPLWVRLGFIGAAVI
                     VPGVSLIAVVVAYVVLGIALPERTTL"
     gene            complement(3301..4038)
                     /locus_tag="VGR29_01320"
     CDS             complement(3301..4038)
                     /locus_tag="VGR29_01320"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEV2072255.1"
                     /translation="MHGHDASLPERALRLSRRSLLGVASGALVAGMLRSTTAQESTPD
                     STPELVPIQSTTVGELPSAGGMRPGPVGQQSPRPSAVNPTDPVNITVEKAAINADIVT
                     MEIVNGVMQNPPGPWVVAWYRQTATLGEQGNVVLAGHVDYWDVGPSVFFNLRDLVAGD
                     IVSLTGENGTRYDYAVEWSEVFDIDALTGGKLQEVVGLTERPAVTLITCGGEFDYANG
                     EYLSRMVVRGTLVERIGTPEATAEAGA"
     gene            4163..5146
                     /locus_tag="VGR29_01325"
     CDS             4163..5146
                     /locus_tag="VGR29_01325"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV2072256.1"
                     /translation="MNTTMMPDQSTARALLRQIGMDVPEKRITPVFGGTINTTFRIDN
                     GDDGQLILRIAPSDAEVEAGPSWMTSHGLQREQVTIGLLSNIDHLLPRTVGFDESREY
                     IDRDWVLQTSVHGQQWQEARKALTAAEEQDLWRELGEVTRQIHAVTGEEFGPPLTGLG
                     CATWSDLVRWDVAGFSVDAHRYGLEQEPFEQLQGLVDDAAPVLNEITEPRLVHSDLNE
                     RHIFIAPSSDGRPHITGLIDFEFARFADPYSETIFIDEALLPSHDGRDVALCEGYKCE
                     KPTHEDIVRRHIYSLVAMGWTVMDLVRRDQPKQVPALLERMQIMTREAREL"
     gene            complement(5161..6105)
                     /locus_tag="VGR29_01330"
     CDS             complement(5161..6105)
                     /locus_tag="VGR29_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV2072257.1"
                     /translation="MEIITGLGAIIIVFLVLVLFSAIKVVQEYERGVVFRLGRLVGAR
                     GPGLILLIPFIERMQKIDLRTVTMDIPAQEVITQDNVTVRVNAVAYFRVVDPNAAVVN
                     VADYIRATSQIAQTTLRSVLGQSSLDDLLGQRETINQRLQQIIDDQTEPWGIKVSVVE
                     VKDVELPQTMQRAMARQAEAERDKRAKIIHAEGEFQASAQLSEAAEVINRNPVTIQLR
                     YLQTLTEIGAEKNTTIIFPIPIDMVTAFMNKMTGDDDDKAPRARPEPPQAPRGGSGRD
                     VPASKPRPDLKGMPPRPTLLQEGQNPSPQRPKPPTDKA"
     gene            complement(6208..7629)
                     /locus_tag="VGR29_01335"
     CDS             complement(6208..7629)
                     /locus_tag="VGR29_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEV2072258.1"
                     /translation="MSPHSLPRYSSLSGAPLPLLCLVLGTLLMLFQPVVAQDNASTVR
                     VISIDGTITPAMASYVDRAMEGVQRDNVEAVVLRIDTPGGLSTAMDDIVDDILHSSVP
                     VIAWVGPSNARAASAGVYITYAAHIAAMAPGTNIGSASPIQLGGDSEDTGESTAERKA
                     MNDAAARIENLARLRDRNVDWAISAVRDAENITADKALELGVIDLTASNIDVLLKDVD
                     GMQVELASGETVTLDTADIAQTSESMNVFEQFLQLISDPTIAYLLLSFGALGIFLELS
                     NPGGFVPGIVGVVCIILGLYALGTLPVNWTGMLLIALAFALFFIDLFVTSFGLLLIGG
                     LASFIIGSYMLIDESVPGYEGVSRPVIWTSAALILFSATLIGAFVLKSMRRTPATGKN
                     ALIGQVAEVRSPLAPSGMVYVEGEYWSATAAGLPANTPLPVGSKVEVTDVQGLKLTVQ
                     ATAKASHDDVAKTSRRETIVSVP"
     gene            7845..8354
                     /locus_tag="VGR29_01340"
     CDS             7845..8354
                     /locus_tag="VGR29_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV2072259.1"
                     /translation="MMRSPLFSDLVSLRNTVDQLFRESPFSDAFDTLWSRAETSGGTV
                     ARPMPLDVYADDENVFIVAAVPGMRPDDLQLTVQKNTVTLSGTVRNVMDSEEGKKATW
                     YIHELASGSYQRSVTLPFPVDADHAEAHFDHGILRVVLPKAEASKPKQISIQTGQNQA
                     IESGSTQQS"
BASE COUNT         1693 a         2365 c         2659 g         1661 t
ORIGIN      
        1 tcgagatcat tccgcgtgtc gatgtcctct tcgcgtcgcg cggggcactc cagaccttct
       61 acgggcttga gggagcgtat gaggatgtcc tgcgtcaggc gatagagaag ctgggggtcg
      121 cggcggtgac agtgaaccgg aagcgggcca agggaagtcg ccgattgaag ctgacgagta
      181 tggcaatggg gcaaagcggt gtgttggcat cctcggaacc tcgcgatatc gaggtgcttg
      241 accggttggg cgggggagat gcctttgctg ccggcttcct cgcatcctat cttgataatc
      301 cgcatgggct gacacgagcc gtttcgctgg gggccgccgc ttgcgcactg aagcacacca
      361 tgccaggcga ttttctctgc gcgacgaagg aagagatcga ggacgccgta aagagcgtcg
      421 acgcaggcgt cttgcagcgg tagggagaat gggagaacgt tcccgtgccc cggtcagtaa
      481 ggttgactca atcagttgcc cgtaatgaac aggagttacc tgcctggaaa aatgggctcg
      541 ttggacgcgc cagctggagt tggaatattt gctgtcgcgc catcacttga tgggccaata
      601 atgtgcgctt gcccgggcac gccaagcaga tgtgcggcag cctcgtaggc ggcatgaacc
      661 tccacagcct tctcaagtgt ggcaagcgcg aggacggttg ggccagcgcc actcaaaact
      721 gcgccgatcg ctccagccct cttcgcggcc tccttcatgg cattgagatg cgggaagagc
      781 cgtgcccggt atggctcatg caaccgatcg tgcatcccgg cggcgatcag tgcgtgatcc
      841 tgtaatttga ggcccatcgc gaggccggcg acagtcgcga cattgaaccg ggcgtccgtg
      901 tgcggcacgg tgtttggcag ggccgcacgc gcggcgtggg ttgcaccggt cgcctcggga
      961 atgaagagca cgcaggtgag acccaagtcc tctttgtgcc acagccgggt cacccctgtg
     1021 atgtgcggca cggccataat ggcgccaccg tacatcgtcg ctccgacgtt gtccccgtgt
     1081 ccctcgatgc gccaggccat ctggaagaga tcatcctgac tcaggtcgag gtccagaagg
     1141 cggttggccg cgatcaaccc gacgacgagc gccgccgccg atgatcccag cccgcgtgag
     1201 acaggaatgc cggtataggc ggtgagcgag aacgcgggga gtttgcggcg gtactccctc
     1261 gccagcattc gcatggcggt cagcgtcagg ttttcgtggc cttcgagcag cgcggattcc
     1321 ggcccctcat tgatcaccga accgggttca ccatcggtat cgatggtgac ctcgttccag
     1381 atatccagcg caatgccgat cgtgtcgaac ccggggccca gattggcgga agaagcggga
     1441 gccctaaccg agaactgcat gatccagcac cctcttcaat gcttcaagtt gggccacgac
     1501 cttgactggc cggtttgcgc gtggacgcgg ttcaccctca aggtcctgga gatggtagga
     1561 gaccacagcc tcggtgtcct tgagtacatt gccggtcagg accccgacga cggtggcgcc
     1621 aggatcaatc tccccctggg tgacaagctt gcgcactccg gcaagcgacg ctgcggatgc
     1681 tggctcacag ccgatgcctg cggcatcgat ggtggctttg gcgtcaagaa tctcctcgtc
     1741 ggtgacgctg gtcacgatgc cgctcgtaag ctcgatcgtt cggcgggcgc ggtcaacact
     1801 tgccggggcg ccgatcttga tcgccgtggc aatggtgtca gcctgtaccg gttcataatg
     1861 gtcgaagccg gactcataga acgcagcgaa gggtgcggca cctgcggcct ggatggttgc
     1921 aatacggggg agcgtgtcga tcagtccgac ctggaaggct tccgagaggg ccttgccgaa
     1981 cgcggccgta ttgccgagat tgccgccggg cagggcaatg atatcgggaa cctgccagcc
     2041 gagggtgtcc aggatctcga aaacgatcgt cttttgtccc tctggccgga aggggttgac
     2101 ggagttggcg aggtagacat cgtaggcgtc cgtgagctgg cgcaggagct ggagcgcaac
     2161 gtcgaaatcc ccttcgactt ggacgacagt tgcaccatat gcgatcgtct ggctcagctt
     2221 gctggcgctg atcttcccat ccgggatcag gaccatggcc ggtataccgg cggtcgcagc
     2281 ataggaggca agtgaggcag aggtgttacc ggtcgaggcg caggcgacga tccgtgcgcc
     2341 tgtcgccttc gcatggctaa gggcaacggt catgccccgg tccttgaagg agccggtcgg
     2401 gttgtgtccc tcgtgcttga cgccaaaacg tccaccggaa aggccggcgt acgcgccaag
     2461 gcgctcgtcc caatagagcg gggtgttgcc ttcgtagcgg gtgacgatat gctcgtcggc
     2521 aatgggtggc aggagcggtc ggaaacgcca tacgccggag agcgcctcga atgatcctct
     2581 tgcagcgtcg aacgatgcag gcgtgagttg ctgtgcaacg tcgatccagg cgtcgagcaa
     2641 tcctccacat gtcgggcagg tggtgaggga ctgataaccg tcaaaggtcc gtccacacgc
     2701 catgcaggtc accgacagac cgggggacga agatacggac gtgctctcac cgggcgcggt
     2761 catgtgacgg gagttcctct cttggcgtgg cttggctggt tctcagggat gacgggcagc
     2821 gcggcatcgg gcgcccgacg atggcgtgag cataccacca accctggcca ggacgagagg
     2881 atgaatgttc cgtccctgtc cctagagtgt agtgcgctcc ggaagggcga tgccgagcac
     2941 gacataggca acgacgacgg caataagtga cacgccgggg acaattactg ctgcgccgat
     3001 gaacccaagg cgaacccaca gggggtcaac accgaatttg taggcgatac ccgcgcagac
     3061 gccgcggatc cagcgattct ttctggagcg ttcgatccca atgtcaaggt gcatactctg
     3121 gtctcctcat ttccgacgcg aatccgcgat gattcgccct ttcgtggctc aagcataggc
     3181 caggaactcc aggggcggca tgggtgataa ccggaaatcg cagagacagg ataacgggac
     3241 gcagtgggct ggagagggtg ccaagcccaa acccgaaaga gacgatcccc tcggcatccc
     3301 tcaggctccc gcttcggccg tggcttcagg agtgccaatg cgttccacca gcgtgccccg
     3361 cacaaccatc cgggagaggt attcgccatt ggcgtagtca aactcgccgc cgcacgtgat
     3421 cagcgtgacg gctggtctct cggttaagcc aacaacctct tgcaacttgc cgcccgtgag
     3481 cgcatcgatg tcgaagactt cagaccattc gacggcgtag tcgtagcgtg tgccgttttc
     3541 tccagtcagg ctgacgatgt ctcccgcgac aagatcccgc agattgaaaa agaccgatgg
     3601 accgacatcc cagtagtcga catggccggc aaggacgaca ttgccctgtt ctcccagcgt
     3661 ggcagtctgg cggtaccagg caacaaccca gggacctggc gggttctgca tcacgccgtt
     3721 gacgatttcc attgtcacaa tatcggcgtt gatcgcggcc ttctcgacgg tgatattgac
     3781 tggatcagtt gggttgacgg cggagggccg aggggactgt tgaccgactg gaccgggccg
     3841 catgccgcca gcggacggca gttctcctac agtggtggac tggatgggca ccagttccgg
     3901 ggtggaatcc ggcgtcgatt cctgcgcggt cgtggaccgc agcatgcctg caaccagcgc
     3961 accgcttgcc acgccgagta gggatcgccg gctcaggcgg agggcgcgtt ccgggagcga
     4021 agcgtcgtga ccgtgcatct tggatgatct cctggttgca gtgtgcggct tgaatgcctg
     4081 tacttctact atcttaggac gaaccagcgt cccgtttggt gtcgcactgg cagaagcatt
     4141 gaactatgag gcgttgtatc tgatgaacac gaccatgatg ccagatcaat caacggcacg
     4201 ggcgctgctg cgccaaatcg gcatggatgt ccccgaaaag cgcatcactc cggtgttcgg
     4261 cggaacaatc aataccacgt tccgcatcga caatggagat gatggccagc ttattttgcg
     4321 cattgcccca agtgatgcag aggtggaagc cggtccaagc tggatgacct cgcatggctt
     4381 gcagcgggag caggtcacaa ttgggctgtt atcaaacatc gatcatctgc tacctcgtac
     4441 agtgggcttc gatgaatccc gggagtacat agatcgggac tgggtcctgc agacctcagt
     4501 gcatggtcaa caatggcagg aggcgcgcaa ggcccttact gcagcggagg agcaggatct
     4561 ctggcgagag ctcggcgaag tgacgcgtca aatccatgcc gttacggggg aggagttcgg
     4621 cccacccctg actggcctgg ggtgtgccac ctggtccgac ctggtgcgct gggacgtggc
     4681 cggtttctca gtcgatgccc accgctatgg ccttgagcag gagccctttg aacagttgca
     4741 ggggcttgtc gatgatgcgg caccagtcct gaacgagata actgaaccgc ggctggtcca
     4801 cagcgacctg aacgaacggc atattttcat tgcgcccagt tcagacggcc gcccgcacat
     4861 cactgggctc atcgacttcg agttcgcccg gttcgccgat ccgtattccg agaccatctt
     4921 tatcgacgag gcactgttac ctagccacga cgggcgagat gtggcgctgt gcgagggcta
     4981 caagtgtgaa aagccaacac atgaagatat tgttcgaaga catatttaca gcctcgtcgc
     5041 catgggttgg accgtcatgg acctggtgcg acgggatcaa ccaaagcagg taccggcgct
     5101 tcttgagagg atgcagatca tgacacgtga ggctcgggag ttgtaggctc ggcagatctg
     5161 ctaggccttg tcggtcgggg gcttggggcg ctggggagac gggttctgcc cctcctggag
     5221 gagggtcgga cgcggtggca tgcccttcaa gtcaggtcgt ggtttcgacg caggtacatc
     5281 ccgaccggat ccgccgcgag gtgcctgggg aggctccggg cgcgcacgtg gtgccttgtc
     5341 gtcgtcgtca ccggtcatct tgttcatgaa tgcggtcacc atatcgatgg ggatcgggaa
     5401 gatgatagtc gtgttcttct ctgcgccaat ttccgtcagc gtctgcagat accgtaactg
     5461 aattgtgaca gggttccggt tgatgacctc ggcggcttcc gaaagctgcg cgctggcctg
     5521 gaactcgcct tcggcgtgga tgatcttggc acgcttgtcg cgctcggctt cggcctgtcg
     5581 tgccatcgcg cgctgcatgg tctgcggcag ctcgacatct ttcacctcga caacgctcac
     5641 cttgataccc catggctcgg tttgatcatc gatgatctgc tgtagccgct ggttgatcgt
     5701 ctcgcgctgg ccaaggagat catccagcga cgattggccg agcacggagc ggagggtagt
     5761 ttgggcgatc tgggacgtgg ctcggatata gtcggccacg ttcacgaccg ccgcattcgg
     5821 atcgacgact cggaaatacg cgactgcgtt cacacggaca gttacgttgt cctgagtgat
     5881 gacttcctgc gccggaatat ccatcgtgac agttcgcagg tcgatcttct gcatccgctc
     5941 aataaagggg atcaggagga tcaatccagg accacgggcg ccgacgaggc ggccaagccg
     6001 gaagacaacg ccacgctcgt attcctgcac caccttgatg gcggagaaga gcacgagtac
     6061 gagaaagaca atgatgatgg cgccgaggcc ggtaattatc tccatgcact cctcaagatt
     6121 tgtgtaccgc ggccactgga aatcgtgggt gaagacttag tcaactaatg gtaacggttc
     6181 ccggtgatag caacgtttgt cgggatgtta tggcacggaa acgatggtct cccgacgtga
     6241 cgttttcgca acgtcatcat gtgaagcctt cgcggtagcc tgcacggtga gcttgagccc
     6301 ttgcacgtca gtcacttcga ctttgctacc tacgggtagg ggtgtattcg ccggaagccc
     6361 tgccgccgtc gccgaccagt actctccctc aacataaacc atgcctgatg gtgccagggg
     6421 acttcgcacc tcggcgacct ggccaatcaa tgcattcttc cctgtggcag gtgttcggcg
     6481 catggatttc aggacaaaag caccgatgag cgtcgcagag aagagtatca gtgctgccga
     6541 ggtccagata actggccggg acacaccctc gtaaccggga acagactcat cgatcagcat
     6601 gtaggaaccg atgatgaacg atgccagccc accgatcagc aagagcccga atgaggtcac
     6661 gaagagatcg atgaagaaga gggcgaacgc gagtgcgatc aggagcatgc cggtccagtt
     6721 cactggcaat gtgccaagcg catacaggcc cagaatgata cagacgacgc caacgattcc
     6781 gggtacaaac ccgccgggat tggagagttc caggaagatc cccagcgcac cgaatgagag
     6841 caggagatag gcgatcgttg gatcggagat cagctgcagg aactgttcga agacgttcat
     6901 ggattcgctg gtctgcgcga tatcagcagt atcgagtgtc accgtttcac cactggccag
     6961 ctctacctgc atgccgtcaa catccttgag caggacgtca atgtttgatg ctgtcagatc
     7021 aatcacgcca agctcgagtg ccttgtcggc tgtgatgttc tcggcatccc gtacagcgct
     7081 gattgcccag tccacgttgc ggtcgcgtag ccgggccagg ttctcgatgc gcgcggcggc
     7141 atcgttcatg gcctttcgtt cggcggtaga ctcgccggta tcctcgctgt cgccgccgag
     7201 ctggatcggt gaggccgagc cgatattggt accgggtgcc attgccgcga tatgggcagc
     7261 ataggtgatg tagacgccgg cagaggcggc gcgggcgttc gaagggccga cccacgcaat
     7321 cacggggacg ctgctgtgca ggatgtcgtc gacgatgtcg tccatcgcag tgctgagccc
     7381 accgggtgtg tcaatgcgca gcacaaccgc ctcaacattg tcgcgctgga caccttccat
     7441 cgcgcgatcg acgtaactcg ccattgcggg agtgatcgtg ccgtcgatgc tgatgacacg
     7501 aacggttgag gcgttgtcct gtgccaccac tggttggaag agcatcagaa gcgtgccaag
     7561 aaccagacag aggagcggca acggggcgcc agagagggat gagtaacgtg ggaggctatg
     7621 ggggctcatg cggcggctcc tgttcgtagg tgttccctca ctagtatagg gcggtgacct
     7681 gaccaccgac ttcgcctgtg acaaaacacc agaatgttga cacagagtgc atcaggatct
     7741 tgacatgtga tcggaagatg aatagaatga aatcacggta aaggctaaca gccaccgttg
     7801 tttggttcgg atccgttcgg attgaatgat ggaggtgatg gtccatgatg cgttcccctc
     7861 tcttcagtga cctggtgagt ctgcgaaata ctgttgacca gctcttcagg gaaagcccct
     7921 tcagtgacgc attcgatacc ctctggtcgc gcgcggaaac cagcggcgga actgtcgccc
     7981 ggccgatgcc gctcgatgtt tacgcggacg atgagaatgt cttcatcgtt gcggccgtgc
     8041 ctggcatgcg accggacgac ctgcagctga ctgtccagaa gaacaccgtt acactgtccg
     8101 gcactgttcg gaacgtgatg gactcggaag aaggcaagaa ggcgacctgg tacatccatg
     8161 agctcgccag cggatcgtat cagcgcagcg tgacgctacc gttcccggtg gatgctgatc
     8221 acgccgaggc ccatttcgac cacggtattc tccgggtggt cctgccgaag gctgaggctt
     8281 cgaagccgaa acagatctct atccagacag gccagaatca ggcgattgag tcgggcagta
     8341 cgcagcagag ctagttcatc actgtgatgc ggcacaca
//
LOCUS       DASYWM010000033         6244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_489108, whole genome shotgun sequence.
ACCESSION   DASYWM010000033 DASYWM010000000
VERSION     DASYWM010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489108"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..926
                     /locus_tag="VGR29_01345"
     CDS             126..926
                     /locus_tag="VGR29_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2072260.1"
                     /translation="MEADRTQATAATTSTQSVWEHGEDPAASQGDPILRIENIDAAYG
                     KVRILHGVSMVVHPGEAVSIIGPNGAGKSTVLKAVIGQLTPSSGAITFNGINVVGMRP
                     EQIIRHGMAVVPQGRIVFERMTVVENLEMGAYSLKDKSRVPVLMERIFDLFPRLAERK
                     RQEAGTMSGGEQQMLAMGRGLMSDPKMVLMDEPSLGLAPMFVELIFEQIGVLKKNGLT
                     LLLVEQNAVKSLSISDRAYVLELGRNRFTGSGEALLADPRVRQLYLGG"
     gene            complement(1040..1603)
                     /locus_tag="VGR29_01350"
     CDS             complement(1040..1603)
                     /locus_tag="VGR29_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV2072261.1"
                     /translation="MLTRSPRTGSISWAQGAVGDPDVVFLDTETTGLGAHAEIVDIAV
                     IDRDGHVLMDTLVCPTRGIPREASNIHGILDHHVARAPAWNQVHIELMSILRGRRVIV
                     FNAAYDQKMIRQCCSHFRLMPPSCAWECAMLAYAEYVGERSEWGRGYRWHRLEKAATA
                     FGIVPGGHRARADAEACRQVVLRMAMG"
     gene            complement(1714..2757)
                     /locus_tag="VGR29_01355"
     CDS             complement(1714..2757)
                     /locus_tag="VGR29_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072262.1"
                     /translation="MRIDDARIVARDWVARHAVNVPGFRGAYIAGSTNGLPGEAILPA
                     TSDIDIMIVLDAVDVPAKPGKFLYHGVLLEATYLSMDLVANVEQVLGHYHLAASLRRP
                     VILADPDGHLTRLQPEVEAAFSRRKWVERRCEQARETALTRIASVIEGRAFEEQVMAW
                     LFAAGGLPHMLLVAGMRNPTVRRRYEATRVLLVEYGYEAFYPGLLALLDPEGISAEQT
                     MRHLVTVGEAFDVASSVISTPFPFAADISAAARPVAIDGSREMIEEGRHREALFWVVA
                     TFARCQMVLHADAPPPMSTRWDGSFQDLLADLGIRTFADLDQRCEHIREALPQVDSIA
                     VDVMDASPEIHDD"
     gene            2901..3539
                     /locus_tag="VGR29_01360"
     CDS             2901..3539
                     /locus_tag="VGR29_01360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2072263.1"
                     /translation="MPLKVLFVRHGESQANLDQMFANRVGIPGDLTPAGVAQAHALAE
                     SLAPEPVTHVYTSPLDRARQTAEIIAKTLGVPATITDALREYDVGDFEGKPYSGSDAW
                     RWERYERIERAWRDGQHEARHSEGESLFDLRDRFLPFMDRLVEHHDKSEILIAVGHGG
                     LFRAVLPFLFASLPSPEVYAQPLRHGDMVVAVHDRGTWRCEGWGEQQASGAG"
     gene            complement(3547..5070)
                     /locus_tag="VGR29_01365"
     CDS             complement(3547..5070)
                     /locus_tag="VGR29_01365"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEV2072264.1"
                     /translation="MAALIIVGLLLLVGSLVVLFVSDERDDGMDSGTPDVTADLVGAT
                     LVASPPDTVPIASASPLAPGATPVASPGATPLASPGATPIPPLLSGQCREGCLIRVPR
                     NDLTVALLKETGERPSYESDTWVWTIVSAKTVMDLQASDVPVFLVRDSPETLYLYATR
                     LPKDQSGSRVVREFGEVLDSVDGHSIVRVDDVPANVTDLIAARVWVEKVKPAAPGLAS
                     QSPAGIGEPISEVDLGVLLPEVDAEEIEQTIAELQEMSSTDGTGIGTRHYTSTGNAMA
                     AEYLFTRLESYGLTVWYEDFVTPEGLLSTNVIGEIPGQDPGSIYAVMAHLDSVAADTS
                     SAPGADDNATGLAASLEIARILSGYELKYPVHIILVNAEETAILGSDAYARDAVQEET
                     PLEGVFNLDSIGSDRNGPRVYLNADEGSAWMMDLLVRINDGYGLGQELWVRQNPEIIA
                     DDNMLRAQGIEAVLVARELFGMSPVHHTADDIKENVSIPYTIEATQLVLLAIAALVQ"
     gene            5504..6025
                     /locus_tag="VGR29_01370"
     CDS             5504..6025
                     /locus_tag="VGR29_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072265.1"
                     /translation="MFHRIRVSALSLILLLAFGTMLPAKAQPTPTAGPPATLQVAFPV
                     EKLPSPEAEVHFLRYTFGPGAQLPAAPQIGSTLLAVESGEITLTTDQPVSVKNGGQLA
                     TPSPATSHAGETTLGPTQGALIPDGTLVSMRNDSDQEAQLLVLRVFSQEQNPVVNGAD
                     GSPAAESSGVNVQ"
     gene            6041..>6244
                     /locus_tag="VGR29_01375"
     CDS             6041..>6244
                     /locus_tag="VGR29_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072266.1"
                     /translation="MTFPAWSGVLLIEREVVGTNRSAASSTHNGIEAGLLEQGSAQVL
                     FMTGSTSIRPGMRNIPEDATPPAP"
BASE COUNT         1233 a         2013 c         1835 g         1163 t
ORIGIN      
        1 cgaccatgaa atggcgatca tgatggagct gtgcgagagc atcttcgtgt tcgattacgg
       61 ctcggtgatc gcgcacggcg cacctgccga ggtccgcgag gatcgccgtg tgatggaggc
      121 ctactttgga agctgaccgg acacaagcca cggcggccac aaccagcaca caatcggtct
      181 gggagcatgg cgaggatccc gccgcgtccc agggggatcc gatcctgcgc atagaaaaca
      241 tcgatgccgc ctatggcaag gtccggattc ttcacggtgt ctcgatggtc gtgcatcccg
      301 gcgaagcggt cagcatcatc gggccgaacg gcgccggcaa atcgaccgtg ctcaaggcgg
      361 tcatcgggca gctgacaccg tcttccggtg caatcacctt caacggcatc aatgtcgtcg
      421 gcatgcgccc ggagcaaatc atccggcacg gcatggcggt ggttccccag gggcggatcg
      481 tttttgagcg gatgaccgtc gtcgagaacc tcgagatggg ggcatactcg ctgaaggaca
      541 agagccgggt gcctgtgctg atggagcgga ttttcgatct ctttccgcgt ctcgccgagc
      601 gcaagcgaca ggaagcgggc acgatgtccg gcggcgagca gcagatgctc gcgatgggcc
      661 gtgggttgat gagtgacccg aagatggtcc tgatggacga gccctcgctc ggccttgcgc
      721 cgatgttcgt cgagcttatc ttcgaacaaa tcggtgtgct caagaagaat gggctgacgc
      781 ttctgctggt cgagcaaaat gcggtcaagt cgctctcgat cagcgatcgc gcctatgtgc
      841 tcgagctcgg ccgcaaccgc ttcaccggct ccggcgaagc actcctcgcc gatccccgcg
      901 tacgccaact ctatctcggc ggataattcc ggtcttccat ccctcagagc cgattccgct
      961 cgttgtaggg gtcaactcca tgttgaccct cccccacgca cccgtgctgc ggccattgct
     1021 gtcacccacg acgccagcgt caccccatcg ccatccggag aacaacctga cgacatgctt
     1081 ccgcgtcggc gcgagcccgg tgccctcccg gcacgatccc gaaggccgtg gcggccttct
     1141 ccagcctgtg ccagcgatat ccgcgccccc actcgctccg ctcaccgaca tactccgcat
     1201 acgccagcat tgcgcactcc caggcgcagc tcggcggcat caggcgaaaa tgcgagcagc
     1261 attgacggat catcttctgg tcgtaggctg cattgaagac aattacccgc ctacccctca
     1321 ggatggacat gagttcgatg tgcacctgat tccacgcggg agcacgagca acgtggtggt
     1381 caaggatgcc gtggatgttc gatgcctcgc ggggaatgcc gcgcgtaggg caaacgagag
     1441 tgtccatcag gacgtgacca tcccggtcta tcacggcgat atccacgatc tcggcatgag
     1501 cacccagccc agtcgtctcg gtgtcgagga agaccacatc aggatcaccg acggctccct
     1561 gggcccacga aatcgaaccg gttcttggac tccttgtaag catgcgtttt ctccctgaca
     1621 gtgccccacc gtccgcaaat ctgaactttg tgtacgtaca caataggagg cggtgttccg
     1681 ctcgtcaagg agcgagagct cagtttccag ccgtcagtcg tcatgaatct ccgggctcgc
     1741 gtccatgacg tcgacggcga tgctgtctac ctgcggcaag gcttcccgga tatgctcgca
     1801 cctctgatct aggtcggcga acgtccggat accaaggtca gcgaggagat cctgaaacga
     1861 accgtcccat cttgtgctca tgggcggcgg tgcatcggca tggagcacca tctggcaacg
     1921 ggcgaacgtc gcgacgaccc agaacagtgc ctcgcggtgg cgcccctcct cgatcatctc
     1981 ccggctcccg tcgatcgcca ccgggcgcgc ggctgcggag atgtcggcgg caaaggggaa
     2041 cggcgtcgag atgacggagc tggcaacatc gaatgcctct ccaaccgtca caaggtgccg
     2101 catggtctgc tcggcgctga tcccctccgg gtcaagcaat gcgagcaagc caggataaaa
     2161 cgcctcgtat ccgtattcga ccaggaggac gcgggtggcc tcgtatcgac gccgcaccgt
     2221 agggttcctc atcccggcga caagaagcat atggggcagc ccaccggcgg cgaagagcca
     2281 tgccattacc tgctcctcga acgctcggcc ctcgatcacg ctggcaatcc gcgtcagcgc
     2341 tgtctcgcgt gcctgttcgc agcgccgctc cacccatttc cgtctcgaga atgctgcttc
     2401 gacttcaggt tgcagacgcg tgaggtgccc atccgggtcg gcaagaatca ccggccttcg
     2461 caaactcgcc gccaggtgat agtgccccaa cacctgctca acattcgcga ccagatccat
     2521 cgacaggtat gtagcctcaa gcagcacccc gtgatagagg aacttgccgg gctttgcggg
     2581 aacatcaacg gcatccagca cgatcatgat gtcgatgtcg gacgtcgcgg gaaggattgc
     2641 ctcgccgggg aggccgttgg tcgagccggc gatgtaggcg ccgcgaaaac cgggcacgtt
     2701 gacagcgtgc cgggccaccc aatcccgcgc cactatccgg gcatcatcga ttctcatgcc
     2761 tgcaacccgt gcataccgcc ttcccctagt ctgaatttaa ttggagtaat ttcccgccca
     2821 tccctgcatc ttgcccgctt cctgtaccat catggacgtc atggaccgct cgcgccatat
     2881 gtaaaatccg gagggccacc atgccgctga aggtcctgtt cgtgcgccat ggcgaaagcc
     2941 aggcaaacct ggatcagatg ttcgcgaacc gcgtcgggat cccgggcgac ctgactccag
     3001 cgggcgttgc ccaggctcac gcattggcag aatccttggc gcccgagccc gtgacgcacg
     3061 tttacaccag ccccctcgac cgtgcccgcc agacagcgga gatcatcgcc aaaacgctcg
     3121 gcgttcccgc cacgatcacc gatgcgctgc gcgagtacga cgtcggcgat ttcgaaggca
     3181 aaccgtactc tggtagtgac gcgtggcgct gggagcgata cgagcggatt gaacgggcct
     3241 ggcgggacgg acagcatgag gcccgtcact cggagggaga aagtctcttt gacctccgtg
     3301 atcgcttcct tcccttcatg gaccggctgg tagagcacca cgacaaatca gagattctca
     3361 tcgcggtcgg ccatggaggt ctctttcggg ccgtgctccc attcctcttc gcgtccctgc
     3421 catcgccgga ggtctacgcc caaccccttc gacatggcga catggtggtc gccgtgcacg
     3481 atcgcgggac ctggcgttgc gaagggtggg gggagcagca agcatcgggt gctggatgat
     3541 cggccattac tggacgagtg cggcgatggc caggagcacc aattgtgtcg cctcgatggt
     3601 atacggaatc gacacattct ccttgatatc atcggcggtg tggtggaccg ggctcatccc
     3661 gaataactcc cgcgcgacca gcactgcctc gatcccctgg gcccggagca tattgtcatc
     3721 cgcgatgatc tccgggttct gccgtaccca gagctcctgg ccaaggccgt acccgtcgtt
     3781 gatccgaacg agcaggtcca tcatccatgc cgatccctcg tcggcattta ggtacacccg
     3841 gggaccattg cggtcggacc ctatggagtc caggttgaac accccttcga gcggcgtctc
     3901 ctcctggacg gcatcccgcg cgtaagcatc cgagccgaga atggctgtct cttccgcatt
     3961 cacgagaatg atgtgtaccg ggtacttgag ctcgtaccca gagaggatcc gggcaatctc
     4021 cagggaggcg gcaagtccgg tggcgttatc gtccgcgccg ggagcggagc tggtgtcggc
     4081 agccaccgaa tcgaggtgcg ccatcacggc atagatggag ccgggatcct gccccggaat
     4141 ctcgccgatc acgttcgtgg acagcagccc ctcgggcgtc acgaagtctt cataccagac
     4201 agtgagtccg tacgattcaa ggcgagtgaa gaggtactcg gccgccatcg cattgcccgt
     4261 gctggtgtag tggcgagtgc cgatccctgt cccatcggtg gagctcatct cctgtaattc
     4321 agcaattgtc tgctcgatct cctccgcatc tacctcggga agcagtaccc ccagatccac
     4381 ctcggagatt ggttctccga ttcccgctgg gctctggctt gctagaccag gagctgcggg
     4441 tttcaccttc tcaacccaca cccgggccgc aatgagatcg gtcacattcg ccggaacatc
     4501 gtcaacgcga actattgaat gtccatccac gctgtccagg acctcgccga actcccggac
     4561 aaccctgctt ccagactgat ccttcggcaa tcgcgtcgca tagaggtaaa gcgtctcggg
     4621 tgaatcccgc accaggaaga ccggtacatc gctcgcctgc aagtccatga cagtcttcgc
     4681 cgagacaatg gtccacaccc acgtatcgga ctcgtaggaa ggccgctctc cggtctcctt
     4741 caggagcgca acagtgagat cgttgcgtgg cacccggatc aggcagcctt ccctgcactg
     4801 tccgctcaag agaggcggga tcggtgttgc gccgggcgag gccaaaggcg tggcaccggg
     4861 agaagcgacc ggagttgcac cgggcgcgag cggcgatgcg gacgcgattg ggaccgtgtc
     4921 gggagggctg gcaaccaagg tggcacctac gaggtccgcc gtcacatcgg gcgtccccga
     4981 gtccatccca tcgtctcgct cgtcgctgac aaacagcacc accaacgaac cgacaagcag
     5041 gagtaacccc acgatgatga gcgccgccac acctcgcgat ttccagctta ctgattcccg
     5101 taatcccagc atcgacggct tcgtttctca agagcagcaa caggagtcgc aaacagaatc
     5161 agagggtgta cgtccacggc ttacatggag tgtagtacca caagagcgct agaagcaact
     5221 attgggcaac cactggttag ggtgatgcac tgcgcgtgcg ttcaaccaac atgaggtgct
     5281 tcactccgca aaaatcccat ggaatgcgat ctggcatgga ttgattgcag ccgtcgctat
     5341 gatggaagga tgcaggcgaa cgctctggcg gcatacgtga tcctcctaca cgggtggatc
     5401 gcgcggtcga tcttccacct tcgccgcact acgatgtgct ggtccctgga ttgtgaacga
     5461 cgcgttccgg gaaagtgcac ctttgcggac aggagctatt gccatgtttc accgaatccg
     5521 cgtctccgct ctctccctga tactccttct ggcgttcggg accatgctcc ccgccaaggc
     5581 acagccgacc ccaacggctg ggccgccagc aaccctgcag gttgccttcc cggtggagaa
     5641 actcccctcg cccgaggcgg aggtccactt cctccgatac acctttggtc cgggcgctca
     5701 actgccggcg gccccacaga tcggctcgac gctcctggct gtcgagtcag gcgaaatcac
     5761 gctgaccacc gaccaaccgg tgagcgtgaa aaacggagga caacttgcaa cgccctcgcc
     5821 tgctacgtcc catgcaggcg aaactaccct tggcccaacg cagggagcgc taatcccgga
     5881 cggcacgcta gtaagcatgc gaaacgacag tgatcaggag gcacagctgc tagtccttcg
     5941 ggttttctct caggagcaaa acccggtggt gaacggcgcc gacggttccc cggccgcgga
     6001 gtcaagtggt gtgaacgtcc agtagatctc ttcccaacgc gtgacctttc cggcctggtc
     6061 cggcgttctc ctgatcgagc gagaggtcgt gggcaccaac agaagcgcgg ccagcagcac
     6121 gcacaatggc atcgaggctg gcctacttga gcaaggaagc gcgcaggtac tattcatgac
     6181 cggcagcacc tcaatccggc ccggcatgcg gaacatcccg gaagacgcga cacctccggc
     6241 tccg
//
LOCUS       DASYWM010000034         3736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_170147, whole genome shotgun sequence.
ACCESSION   DASYWM010000034 DASYWM010000000
VERSION     DASYWM010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170147"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VGR29_01380"
     CDS             <1..218
                     /locus_tag="VGR29_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072267.1"
                     /translation="TELWLTAEGEVIWKKGVDPTIEVELDPEADVALPYTYTGNVVPQ
                     NVFTTLLDNQLIAAYEEVLEAVEATGT"
     gene            417..842
                     /locus_tag="VGR29_01385"
     CDS             417..842
                     /locus_tag="VGR29_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV2072268.1"
                     /translation="MISVESWPRSAVRLWTLTQRFQKFIVVGAIGLAVNQMGLLFLHD
                     IAGLEVKAASPIAIMASMVVTFFLNEVWTWHDRGSGRIIHRAMSYVPINLGGLLINWS
                     ILTYLHDAFGMHYLLANLFGAAAAAVWNFVLNNAITWRA"
     gene            1080..2573
                     /locus_tag="VGR29_01390"
     CDS             1080..2573
                     /locus_tag="VGR29_01390"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2072269.1"
                     /translation="MQNGHHPNGGGDHSSSGSQTVVMGAGPAGLCSAYVLSKAGAHAT
                     VVEAAPFVGGLARTIRRDTEHGEFKFDIGGHRWFTKNEELNDLFREVVAEELLWVNRI
                     SRIYFDGKYVDYPLKITSALKATGPVTAARAVLDYGRVRAQQRLHPTEVKSMEDAYIN
                     QYGNTLYKLFFQAYSEKVWGLKCDKLSGDWVSQRTKGMSLVTAIKDAIIPSKGKVVSL
                     IDQFMYPRDGFGRFSERMADAITASGNEIRLKHAVEKIVLEGNRVKGIRVRTPNGTEL
                     IEGDNFISSIPFTVLVKIMEPAAPQDVIDAADSLTFRDIITVNLMIKKRQVTPDTWLY
                     VHDRNILFGRFHEPKNWSPAMVPSDEYTSLVIEYFCTKGDHIWNMSDEALVNQSVKHL
                     VEDLHFVKPEEVLGGFTLRATKAYPVYDLDYQEPLLKMKNYIQSIENLQYIGRGGSFR
                     YNNTDHSIETGMLAAKNLLGERHDLERVNADEEYHEIKRTEAQSSVV"
     gene            2754..3083
                     /locus_tag="VGR29_01395"
     CDS             2754..3083
                     /locus_tag="VGR29_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072270.1"
                     /translation="MSRRMRTVLWAFATIALFGYIAFGSVPAYTWRFGRLHDDARPVR
                     GIWSVQASNLPDVSRQWLIDGVFYGALIAFLIGVTLGLWYLLTSEDSKASPHQPASST
                     SAGPTSH"
     gene            3107..>3736
                     /locus_tag="VGR29_01400"
     CDS             3107..>3736
                     /locus_tag="VGR29_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072271.1"
                     /translation="MDHDTDRSHPGMAGNESSRQAAFAALLLAALCGWVIWRFGGFDF
                     FQTGSIAGRSRTVVDTFAAVDHPFHATRAATLLRNVQQGDLLRWVGHHQGGYPVEFYP
                     LGVAWFEVVLWFTALGTVPIIAIHKLAVLLIFVMPAIGFWILARGDGLNPWLPILATV
                     LHVTIPGGVWTNGGWTRGGYEELVSWGLVTNVAGATVALIACAALARAVI"
BASE COUNT          736 a         1098 c         1140 g          762 t
ORIGIN      
        1 gaacggagct ttggctgacg gcggaaggcg aggtaatctg gaagaagggc gtcgacccca
       61 cgatcgaggt ggaacttgat cccgaagccg atgtcgcctt gccatacacg tataccggaa
      121 atgtggtgcc gcaaaatgtc ttcacgacgc tgctcgacaa ccagttgata gcggcctacg
      181 aggaggtttt ggaggcggtc gaggccaccg gaacctgaca gcgatggagt gcagagacac
      241 ggggccgacg atacgtcggc cccgtgttgc gtcctgcgca tgatgccttg ctgacagggg
      301 tgcgtggtat tgttctggcg gagacatctg caaaggccat ggactgactg cgcatggtca
      361 ggccaagggt tgtgcagcca tgggtcgggg acctagccaa ggagagtcag gagcggatga
      421 tctcagttga gtcctggccg cgatcggccg tacggctctg gacgttgaca caacgattcc
      481 agaagttcat cgtggtcggg gctattggtc tggcggtcaa ccagatgggc ctgctctttc
      541 tccatgacat cgccggactc gaggtcaagg cggcttctcc catcgccatt atggcctcga
      601 tggtcgttac cttttttctc aacgaagtct ggacatggca cgatcgcggg agtggccgga
      661 tcatccaccg ggcaatgtcc tatgttccca tcaacctcgg tgggctgctt atcaactggt
      721 cgatcctgac atatctccat gatgcgttcg gcatgcatta cctccttgca aacctgttcg
      781 gggccgccgc cgccgcagtc tggaacttcg ttctcaacaa tgccatcacg tggcgcgcgt
      841 agcacgccat gagcccggtt ccgacacacg ctccttccaa ccccaccgaa ttcacctgcc
      901 gtcccttgcg cggtcgggcc gccctgagcg tggtatacat ttagtggctg cgagagaccc
      961 atgaaatggg gccttttggc gtgcctgcaa cggcaatttc gcacccgtgt agatgacaag
     1021 taccggatcg atcctcgatc ccaagggtga cacagacgcc caggaaggaa actccgacga
     1081 tgcagaacgg ccaccacccg aatggagggg gagatcactc ctcctccgga tcgcagactg
     1141 tggtcatggg cgcgggaccc gcgggattgt gttccgcata tgtgctcagc aaggcaggcg
     1201 cgcatgcaac cgtggtcgag gccgctccat tcgtcggagg tctagcccga accattcgcc
     1261 gggataccga gcacggcgag ttcaagttcg atatcggtgg gcatcgctgg ttcaccaaga
     1321 acgaagagct gaacgatcta ttccgtgagg tggttgcgga agaactgctc tgggtcaacc
     1381 ggatcagccg catttatttc gatggcaagt acgttgacta tccgctcaag atcacaagcg
     1441 cgctgaaggc aacaggcccg gtcaccgccg cgcgggcggt tctggattat ggtcgtgtcc
     1501 gtgcccagca gcgcctccac ccgaccgaag tcaagtcgat ggaagacgcc tacatcaacc
     1561 agtatggcaa cacgctctac aagctgttct tccaggcgta ctcggaaaag gtatggggtc
     1621 tcaagtgcga caagctgtcc ggtgactggg taagccagcg caccaagggc atgtcccttg
     1681 ttacggcgat caaggacgcg atcatcccgt cgaagggcaa ggtcgtgagc ttgattgacc
     1741 agttcatgta cccgcgtgat ggattcgggc gcttttccga acgcatggcc gatgcgatca
     1801 cggcttccgg caatgagatc cggctcaagc atgccgtcga gaagatcgtg ctcgaaggca
     1861 accgggtcaa gggcattcgt gtccgcacac ccaacgggac ggagctgatc gagggggaca
     1921 atttcatttc ctccatcccg ttcaccgtcc tcgtcaagat catggagccg gccgcgccgc
     1981 aggatgtgat cgacgccgcg gactcgctga ccttccgcga catcatcacg gtgaacctga
     2041 tgatcaagaa gcgacaggtt acccccgata cctggctcta cgtccacgat cgcaacatcc
     2101 tgttcggccg ctttcacgag ccgaagaact ggagcccggc aatggtgccg agcgatgagt
     2161 acacatcgct cgtgatcgag tacttctgca cgaagggcga ccatatctgg aacatgtccg
     2221 atgaggcgct ggtcaatcag tccgtcaagc acctggtcga ggatctccac ttcgtcaagc
     2281 cggaggaagt gcttggaggg ttcacgctcc gggccaccaa ggcctatccg gtgtatgacc
     2341 tcgattacca ggaaccgctg ctcaagatga aaaactacat ccagagcatc gagaacctgc
     2401 agtacatcgg gagaggcggg tcgttccggt acaacaatac cgatcactcg atcgagaccg
     2461 gcatgctggc cgccaagaat ctgctgggtg agcggcatga cctggaacgc gtcaatgccg
     2521 atgaggaata tcacgagatc aaacggaccg aagcgcagtc cagcgtcgtg tagtaatggt
     2581 gggaatgccc atgcgagttg gaaggccgtt gcgccatcgc agctgcctcc tcgctgacgt
     2641 gtggttccaa acgggtggca atgattcgca gcgagcctga aacccctcca tctccaacgc
     2701 cggatgaccc gtcgtccatg gctgaggaca ccggcgaaag gcgatcctcg gcaatgtccc
     2761 gacgtatgcg gacggtcctc tgggcattcg ccacgatcgc cctgtttggg tacatcgcat
     2821 ttggctcggt gccggcgtac acgtggaggt ttggccgcct ccatgacgat gcccggccgg
     2881 ttcggggcat ctggagcgtt caggcgagca atcttcccga cgtatcccgc caatggctca
     2941 tcgatggtgt gttctatgga gcgctgatcg ccttcctgat tggggtgacg ctcgggctct
     3001 ggtatctgct cacgtcagag gacagcaaag cgtcccctca ccagccagca tcatccacaa
     3061 gtgccgggcc gacatctcac tgatgtcgcg gtcgcatcag tcctcggtgg accacgatac
     3121 tgatcgctcc catcccggaa tggcgggtaa tgaatcgtct cgccaggcag cgttcgccgc
     3181 gctgttgctt gccgcgctat gcgggtgggt gatctggcga ttcggcgggt tcgacttctt
     3241 ccagaccggc tcgatcgcgg gccgttcgcg gacggtggtc gacacgttcg ccgcggtcga
     3301 tcaccctttc catgccaccc gtgcggcgac gttgcttcga aacgtgcagc agggcgatct
     3361 gctgcgatgg gtggggcacc atcagggcgg atacccggtc gagttctacc cgcttggtgt
     3421 ggcgtggttc gaggttgtgc tctggtttac ggccctcggg accgtcccga tcattgccat
     3481 ccacaagctc gcggtactgc tgatcttcgt gatgccggcg attggattct ggatcctcgc
     3541 ccggggtgat ggtctcaacc cctggctacc gatcctcgcc acggtcctcc atgtcaccat
     3601 ccctggcgga gtctggacca acggtggttg gacccgtggc ggctatgagg aactggttag
     3661 ttggggactg gtcaccaacg tcgcgggagc gaccgttgcc ttgatcgcct gtgcagcgtt
     3721 ggcacgggca gtcatt
//
LOCUS       DASYWM010000035         4738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_470444, whole genome shotgun sequence.
ACCESSION   DASYWM010000035 DASYWM010000000
VERSION     DASYWM010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470444"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VGR29_01405"
     CDS             <1..357
                     /locus_tag="VGR29_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072272.1"
                     /translation="EGDRHLFREAVDEYKRLRPFLPGQRFLLTGPLHQDWEIWQFVHP
                     SEEAFALLAFREGGQISGVRVEMCVPAQDRSYLIQRSGADGVVTVSGVELASDGIMLC
                     LPEQRSSDVIWVTAAP"
     gene            complement(678..2000)
                     /locus_tag="VGR29_01410"
     CDS             complement(678..2000)
                     /locus_tag="VGR29_01410"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2072273.1"
                     /translation="MSTPEDAGVKTTHPASADSSGTAATDQPHPPGLLRHRDFLHLWA
                     AQTLSMLGSQITALAIPLLAVVTLQATAFDMGVLAMAASLPTPVFGLVAGVWTDRLRR
                     RPILVGADLGRAVLLGTVPFAVFLDLLTIPYLCAVVVLVAFFTVWFDVAHALLLPSLI
                     HPEQLVEGNSKLEISRSGALIAGPGLAGLLIQAISAPVAILVDAFSYLVSAVLLVRIR
                     TVELPPERAGLKSPVWEEAREGWRAVRQDPRLASMALSLSVFNLFSSMLNALFILFAV
                     RELGIDAAALGLIFAAGSAGFPVGAAAAGWVARRIGVGWAIIYGAVISDLALLLIPLA
                     GIMQDAAVPLLIGSRLIATLTGPVTAINQLSLRQSLTPHRLLGRVNATMLVLALGLTP
                     LGGLLAGGLGQIIGIQATVTVAAIGVQAGFVVLLASPLRTLARVPPTP"
     gene            complement(2143..2925)
                     /locus_tag="VGR29_01415"
     CDS             complement(2143..2925)
                     /locus_tag="VGR29_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV2072274.1"
                     /translation="MTEHRSPHTPIVKTTNVRKRYGELDVLRGVSLSVNQGQVKVIVG
                     PSGSGKSTFLRCLALLEPIDDGHITLDGDEITWLASGGRRKGPGERTLARHRSEVCMV
                     FQHFNLFPHMTAIENVMVGPVHVRGTPKSQARQEAMTLLERVQLSNRANHYPHELSGG
                     QQQRVAIARALAMRPKVMLFDEPTSALDAELVREVIDVMEELVREGMTMVVVSHELRF
                     AHEAADTIVMMADGEIIEEVPPGQFFTSPQHERTRQFLRVVK"
     gene            complement(2922..3635)
                     /locus_tag="VGR29_01420"
     CDS             complement(2922..3635)
                     /locus_tag="VGR29_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV2072275.1"
                     /translation="MTFDWGIFADALTSGAYARGAMVAISVAALAQATALVVGLVIAL
                     LGRSPILPLRIVALGWVWLFRAVPTLLQLLFVWNALPQLIPSLRENWFTPFLAAWLTL
                     SINESAYMAEIIRSGLNAVDKGQTLAGKTLGLRSGQIFRIVILPQMIRVIIPATGNEF
                     ITLLKLTALASVISLRELLTVTSQQVAVNFRFAELYGAATVWYLAIVSIFIVLQARLE
                     RHYQWSSEGRRALSGALNR"
     gene            complement(3663..4577)
                     /locus_tag="VGR29_01425"
     CDS             complement(3663..4577)
                     /locus_tag="VGR29_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072276.1"
                     /translation="MVHAFRAGQRLVSMLRVFLIASVLFSGGRVAADVLAQESTPSAD
                     VLADIGDDGVLTNCIDPAFPPMEYFENQDSTEPIGFDIDLTNELASRLGVEAEFVPLE
                     FTGLLPGLQSGRCDIVASGIYVTAERLETFGAQPYYDSSIVLMTTADNEEITSPEDLS
                     GKTIAVQSGTNYLKMMEDLDARLQSEGKEGITIQTYPKQTDADQQLVVGRADATITQD
                     TEVAFRETAQPGQFKDAYTYPDAQTFAIYYNKDATALGDALERTLEEMRADGTLAEIA
                     SAWNLPEAGAEFTREFDVEATPSASPAS"
BASE COUNT          989 a         1373 c         1477 g          899 t
ORIGIN      
        1 gagggagacc gtcacctgtt tcgagaagcg gttgacgagt acaagcggct gcggcccttc
       61 ctccctggcc agagattcct cctgaccggg ccactgcacc aggactggga aatctggcag
      121 tttgtgcatc catccgaaga agcgttcgcg ttgctggcgt tccgtgaagg tggacagata
      181 tcaggtgtca gggtggaaat gtgtgtaccg gcgcaagacc ggtcctacct gattcagcgc
      241 tcgggagcgg atggagtggt caccgtctca ggagtggagc tcgcgtcgga cggcattatg
      301 ctttgtctcc ccgagcaacg cagcagcgac gtcatatggg taactgcggc tccctgatcc
      361 caacgacatg atgaatgtgg gatggatatt tgaggcatag agcaactgaa gtcccacata
      421 aatggagcag agaactttgt cacaactttg tcacaaatgc agcagaacgg ggtcgcacag
      481 cgccgcatga gcgggactgg tattgcccaa aatccgcatg aatacgcacc tttccagact
      541 aaacagcacg gggtcacatg ggctccccag aatcctgtac ccggcaccaa gcgcacattt
      601 ggtgtgatct gtgtgatctg acggaattct gagggtccgg aagaacttcg ccacagctat
      661 cagggccccg catggcctca tggtgtaggc gggactcggg caagggttct gagcggggaa
      721 gcgagcagga ccacgaatcc ggcctggacc ccgattgccg cgacggtgac cgtggcctga
      781 attccgatga tctgtccgag gccccctgcc agcaaaccgc ccagtggagt aagaccgaga
      841 gcgaggacga gcatcgttgc attcactcgg cccagcaaac gatgcggggt caggctttgg
      901 cgcaggctca attgattgat ggcggttacc gggccggtca gggttgcaat caggcgtgaa
      961 ccgataagca gtggcaccgc ggcatcctgc atgattccgg caagagggat gagaagtaac
     1021 gcaagatcac ttatgaccgc gccataaatg attgcccatc ccacaccgat cctccgtgcg
     1081 acccacccgg cggcggctgc cccaaccgga aaacctgcgc tccccgcggc aaagatcagc
     1141 cccagtgcag cggcgtcaat accaagctct cgcaccgcaa acaggataaa gagcgcattg
     1201 agcatactgg agaagaggtt gaacacgctg agggagaggg ccatgctggc aagtctgggg
     1261 tcctggcgca cggccctcca tccctcgcgg gcctcttccc aaaccgggct cttcaaccct
     1321 gctcgctctg gtggcagttc gaccgtgcgg atccggacca gcagcacggc ggaaaccaga
     1381 taggaaaacg cgtccaccaa gatggccact ggcgccgaga tcgcctggat aaggaggcca
     1441 gcgagaccag ggccagcgat cagtgcgccg gatcgactga tctccagttt gctgtttcct
     1501 tccacgagtt gctcggggtg aatgagtgag ggaaggagca aggcatgcgc gacatcgaac
     1561 cagacggtga agaatgcaac gagaaccacg actgcgcaga gatagggaat ggtgagcaga
     1621 tcgaggaaga cagcaaacgg aaccgtgcca agcagcaccg cacgaccgag atcggcgcca
     1681 accagaatgg gccggcgtcg cagccgatcg gtccagacac ctgcaacaag cccaaagacc
     1741 ggagtcggga gcgatgctgc catggcaagc acccccatat caaaggcagt tgcctggaga
     1801 gtaaccaccg caaggagggg tatcgcgagc gctgtgatct gggaaccgag catcgagagc
     1861 gtttgggcgg cccagagatg gagaaaatcc cgatgccgca agagacccgg tggatgaggt
     1921 tggtcggttg cagcagttcc cgagctatcg gctgaagcag ggtgcgtggt tttcacccct
     1981 gcgtcttctg gcgttgacat cgagtgttcc ttgatgctcg ctcaatgccg ccccagggcg
     2041 ggcctcctta ttatgtccgg gtaggaccaa tcgcaccccg agcaggtttt gggtatggtg
     2101 ccgatttata agtggttcag gatatgaaaa gcgcataggg agctacttga cgacccgcag
     2161 aaactgccga gtgcgctcgt gctgtgggga agtgaaaaac tggcccggcg gtacctcttc
     2221 gataatctcg ccatcggcca tcatcacgat ggtgtcggcg gcctcgtggg cgaatcgcaa
     2281 ctcgtgactc accaccacca tggtcattcc ttcccgaacg agctcctcca tgacgtcgat
     2341 gacctcgcgc acgagttcgg catcgagggc tgaggtcggc tcgtcgaata gcatgacttt
     2401 tggtcgcatg gcgagcgcac gagcgatggc cacacgttgc tgctgcccgc ccgagagctc
     2461 atgaggatag tggttcgccc tgttcgacag ctgcacgcgc tcgaggagcg tcatcgcttc
     2521 ctgtcttgcc tgggactttg gcgttccgcg cacgtgcacc gggcctacca tgacgttctc
     2581 gatcgcggtc atatgcggaa agaggttgaa atgctgaaac accatgcaga cctcggagcg
     2641 gtgtctcgcc agggtccgct cgcctggacc tttccgccga ccacccgatg ccagccacgt
     2701 gatctcatcg ccatcgagcg tgatgtggcc gtcatcgatc ggctcaagca gcgccaggca
     2761 tcgcaggaag gtgctttttc ctgatccgga tggtccaacg atcaccttga cctggccctg
     2821 gttgaccgac agggagacgc cccgcagcac gtcaagctcg ccgtagcgct tgcgcacgtt
     2881 cgtcgtctta acgatgggtg tatggggcga tcgatgctcc gtcatcggtt cagcgctccg
     2941 gatagcgccc gccgcccctc cgatgaccac tggtaatgcc gctcaagccg ggcctgcagc
     3001 acaatgaaga tgctgacgat cgccaggtac cagaccgttg ccgcaccgta gagttcggcg
     3061 aaacggaagt tgacggcaac ctgctggctc gtcacggtca ggagctccct gagggaaatg
     3121 accgatgcga gcgcggttag cttcaagagg gtgatgaact cgtttccggt tgccggaatg
     3181 atcacccgga tcatctgcgg caggatcacg atccggaaga tctgccctga gcgcaggcca
     3241 agcgtcttac cggcaagtgt ttgtcccttg tcgacggcat tcagtcccga gcggatgatc
     3301 tcggccatat acgccgattc attgatggag agtgtcagcc acgcggccag aaaaggcgtg
     3361 aaccagttct cgcgtagcga tgggattagt tgcgggagtg cgttccagac gaacaggagt
     3421 tgcagcaggg tgggcacggc ccggaagagc cagacccacc ccaaggcgac gatccggagt
     3481 gggaggattg gtgagcgccc aagcaaggcg atcaccagtc cgacgaccaa ggcggttgcc
     3541 tgcgccaacg ctgccaccga gatcgccacc atcgccccgc gggcatacgc cccggaggtg
     3601 agggcgtctg cgaagattcc ccagtcgaac gtcatggtgc gcagtactga ccggaatgcc
     3661 cgctacgacg ccggggaagc ggatggcgtt gcctcgacat cgaactcgcg ggtgaattcg
     3721 gcaccagcct cgggcaggtt ccacgcggat gcgatctcag ccaacgtgcc gtccgccctc
     3781 atctcctcga gcgtccgctc aagtgcatcg ccaagcgccg tggcgtcctt gttgtagtag
     3841 atggcgaatg tctgtgcgtc cgggtaggtg tacgcgtcct tgaactggcc tggttgcgcg
     3901 gtttcgcgga acgcgacttc ggtgtcctgg gtgatggtgg catcagcgcg gccaacgaca
     3961 agttgctggt cggcatcggt ttgcttggga taggtctgga tcgtgatgcc ttccttgccc
     4021 tcgctctgca gccgggcgtc aaggtcctcc atcattttga ggtagttcgt gccggattgc
     4081 accgcgatgg tcttgccgga caggtcctcg ggagaggtga tctcttcgtt gtctgccgtg
     4141 gtcatcagga cgatggagga atcgtagtac ggctgcgcac caaaggtttc gagccgctcc
     4201 gcggtgacat agataccgct ggcgacgata tcgcatcgac ccgactggag ccctggcagg
     4261 agcccggtga actctagtgg cacaaactcc gcttcgacgc ccaatcggga cgccagttca
     4321 ttcgtgaggt cgatgtcaaa tccgattggt tcggtcgagt cctggttctc gaagtattcc
     4381 atcggtggaa acgcaggatc gatacagttg gtgagcaccc catcgtcgcc gatgtcggca
     4441 agaacgtctg ccgagggcgt gctctcttgg gcgaggacgt cggcagcgac ccttccgcct
     4501 gaaaacaata cagatgcgat gaggaacact cggagcattg acacgagccg ctgccctgcc
     4561 cggaacgcat gtaccatctc tcgctccttc caagtttggt tgactatgat cgccgaaagg
     4621 atttctctac ccggccctga tgttctgggt gagaacgatc atgcttcgcc aggcaggtac
     4681 tgtcaaggat gatcttgcaa cagtctgcgc cgctggaggt gcgtggaccc atcgggag
//
LOCUS       DASYWM010000036         4319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_19899, whole genome shotgun sequence.
ACCESSION   DASYWM010000036 DASYWM010000000
VERSION     DASYWM010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19899"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VGR29_01430"
     CDS             complement(<1..778)
                     /locus_tag="VGR29_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072277.1"
                     /translation="MTDEAPVQSARQDDRPTELALASAAFLNQVLDLDDDPSTLASDL
                     SAIKRDQNAAIFTIQLESSVGPAAFLVYTYVLDTRGGDGRTGKELFDVGLETLQTAAT
                     RNTPGPRAVAHAETETHGFILATTPGTYRALTGNTREEALEPAPEDLPVTGDTDRIRS
                     EAAEELLKLLRSANDEARTWLNAIRSVSSSQPGDVSDLISFTEAETELALFLFDDSSI
                     GDLLRSLNLLVATAQHHTAEALAGEGETPDDEAQSTTDAAT"
     gene            908..1381
                     /locus_tag="VGR29_01435"
     CDS             908..1381
                     /locus_tag="VGR29_01435"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV2072278.1"
                     /translation="MTHVKSDMAAGFERLDDALVEKLVSVARKFAWNAYVPYSSFPVG
                     AAALTGDGSIIGGVNIENASYGLTVCGERVAIFTAAATGHRTLRAVAVSAPKAAGTTP
                     CGACRQVMNEFKPLEGDLIVVLDSNEGAVQVPLRALLPEAFGPRDLGRVSDLGQD"
     gene            1660..>4319
                     /locus_tag="VGR29_01440"
     CDS             1660..>4319
                     /locus_tag="VGR29_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEV2072279.1"
                     /translation="MPRARLEDLKGYLTTSSVADALGLSAYLIKARIADGTLPPASKI
                     SEAGVLLFDDSWMAQARDILSGTPSRRRRRETKRVSNVPSPEMVLGRAVDEGGWLPTW
                     TEIVGYFNELDDASPRVSVEVLGESTSGLPYIVVAVSAPENLQPNARDLNRDLLSRLW
                     DSRGADEGAVESVLDEAKTVGVILATQHSTEIGAALMTLQLVHDLASSDDPATLEILQ
                     NTISLFIPSHNPDGMQMIQEWYTRVRGTPYVGSDLPFLYHPYTGHDNNRDWFMMTQAE
                     TRHYVSLHNQEHPQAVFDMHQMGRLGPRYMVPPFIDPLDPNQDPVIQQGFAALGTHVA
                     LRLTAADKAGVVTHAIFDNYSPSLAYGNYHGSVDLLSEAASAKLASPVTLKEDELTDE
                     YGFDPKQRLWNQPKPWKGGEWTIGDIVEYNQIAARAFLEHLAKNRRQWLIGYFGINAR
                     SSSRTEKPFGFVIPREQRDPGTTDELLEILQRGLVEVMEATAEIELDGVVWPAGTRVV
                     SLNQPAGPFAKTLLEVQKYPDLRRWPEGPPSHPYDVVGHTLPIQMGVRVVQVDKPIPA
                     EAPLKLVEEQIHYEGEVTEGPSSPRAWVFDERSNASIAAIADLLVEDVPVYRAKEAIG
                     ELGIRAGSIIVPADEKNRALIKEIAQGTGTDARTIDAPLDIPAWRQSRVRMGVYKPWT
                     ASMDEGWARWVLEEFAIEYETLETPDIRQGNLRDRFDVILIPNMSPEHLMEGLPEKNR
                     YKDLNLPEYVGGIGALGMDELRRFVEQGGTLIGVDQGTQALIEGMALPVRNVLRGKKE
                     TEFYCPGSLLRVVMNPDHELGYGLPREPAVLFMESVAFEGFGSEATSVATYPTTNANL
                     SGWILGEEHLQRKSALMEVSYGEGSVVLLG"
BASE COUNT          847 a         1213 c         1396 g          863 t
ORIGIN      
        1 cggtggcagc gtccgtggtg gattgtgcct cgtcgtctgg cgtctcacct tcgcccgcca
       61 gtgcctccgc ggtgtggtgc tgcgcggttg ccacaagcag attgaggctg cgcagaagat
      121 ccccaatgct cgaatcgtcg aacagaaaga gcgccagctc tgtctcggcc tccgtgaagg
      181 agatcagatc ggagacgtca ccaggttggc tgctgctcac ggaacggatg gcgttgagcc
      241 atgtccgcgc ctcatcgttc gcgcttcgaa ggagtttcag gagctcctcc gccgcctcac
      301 tgcgaatgcg atcggtgtca ccggttaccg gcaaatcttc cggagcgggt tcaagcgcct
      361 cttccctagt attcccagtc agcgcccggt aggtccccgg tgttgttgcc aggatgaatc
      421 cgtgcgtttc cgtctccgca tgggccacgg cacgcggtcc aggcgtgttc cgggtggccg
      481 ccgtctggag cgtctccaat ccgacgtcga agagttcttt gcccgtccgg ccatccccgc
      541 cgcgtgtatc aagcacgtag gtatagacca ggaacgcggc ggggccgacg ctcgattcaa
      601 gctggatcgt gaagatcgct gcgttctggt cccgcttgat ggcggagaga tcactcgcga
      661 gcgtcgatgg gtcatcatcc agatcaagga cctgattcag gaacgccgcg gatgcgagcg
      721 caagctcggt cggccggtcg tcctggcgcg cggattgaac gggtgcttcg tcggtcatct
      781 ggatatcctg aggggaagcc cggagcgcgg ggctgcgctg ccggtggaca atggcaccgt
      841 ttactacggc tgagcatacc agttccccta ggcttccgcc tcgcacgacg atgaaaggac
      901 attgcagatg acacacgtga aaagtgatat ggccgcgggg tttgaacggc tggatgatgc
      961 gctggtggag aagcttgtat cggtagcccg aaagttcgcc tggaatgcgt atgtgcccta
     1021 ttcgtcgttt ccggtgggtg ctgcggcgct taccggggac ggttcgatca tcggaggtgt
     1081 gaacatcgag aacgccagct atgggttaac cgtatgcggg gagcgggtcg cgattttcac
     1141 ggcggcggca acaggtcatc gaacgctgcg ggccgtggct gtgtctgcac ccaaggcggc
     1201 gggaacgaca ccatgcggtg cgtgtcgcca ggtgatgaac gagtttaagc cgcttgaggg
     1261 agacttaatc gtggtgctcg acagcaacga aggagcagtt caggtgcctc ttcgcgcatt
     1321 gttgccggag gcgttcggtc ctcgagattt gggccgggtg agtgatcttg gccaggactg
     1381 acctcgcaga cccttgcaat tatcggtatt atcaacataa tcggttgatg acagcgaccc
     1441 cggagggagg gagctggagc tttttggcgt tccgccgcct tgttgattgt cgcatgaggc
     1501 gaaaccacac aggctcgtgc ggcacgcgct gtcgcacaac gccacaatat tatgggttct
     1561 ggagaaactg gctggggtcc ggacccatgc ataacggtgc ccgtatcttt ccgggcggga
     1621 tttttatgcc ccctttgccg tggtcacaag gagtaagtag tgccgagggc aagactcgag
     1681 gatctcaagg ggtatctgac gacgtcatcc gtcgcagatg cgctcgggct ttccgcgtat
     1741 ttgatcaagg cgaggattgc cgatggcacc ctgccgcccg cgagcaaaat ctcagaggca
     1801 ggggtactcc tgttcgacga ttcatggatg gcacaagccc gagatattct ctccggcact
     1861 ccgtcacgac gtcgccgacg tgaaacaaag cgggtatcca atgtcccatc gccggaaatg
     1921 gtgcttggcc gggcggtgga cgagggggga tggctgccca cctggaccga gattgtcgga
     1981 tatttcaatg agctcgatga cgcctcgcct cgagtgagtg tcgaggtgct tggcgagtcc
     2041 acgagtgggc tgccctacat cgtggtggct gtttcggcgc cggagaatct ccagccaaat
     2101 gcgcgggacc tgaaccggga tctgttgagc cgcctgtggg actcccgggg agcagatgag
     2161 ggcgctgttg agtcggtgct ggacgaggca aagacggttg gggtcatcct ggcgacgcag
     2221 cactcgactg agatcggggc ggccttgatg acgctccaac tcgtgcacga ccttgcatcc
     2281 tcggacgatc cggcgacact ggagatcctg cagaacacta tctcactgtt tatccctagc
     2341 cataacccgg atgggatgca gatgatccag gagtggtaca cacgcgtcag gggcacgccg
     2401 tatgtaggct cggatctgcc cttcctctac cacccttaca ccggccacga caataatcgc
     2461 gactggttca tgatgacgca ggccgagacc cgtcactatg tcagcctgca caaccaggaa
     2521 cacccgcaag ctgtgttcga catgcaccag atgggccggc tgggcccgcg gtatatggtg
     2581 cctcccttca tcgatccgct ggatccgaac caggatccgg ttatccagca gggattcgcg
     2641 gcactcggca cgcatgtcgc cctgcggctc acggccgcgg acaaggccgg tgtcgtgacg
     2701 cacgcgatct tcgacaatta ttcgccgtca cttgcctacg gcaactatca cggaagcgtc
     2761 gacctcctga gcgaggctgc aagcgccaag ttggcgtcgc ccgtgacgtt gaaggaagac
     2821 gaactgaccg acgagtacgg gttcgacccg aagcagcgac tttggaacca gcccaaaccc
     2881 tggaagggcg gcgagtggac gattggcgac atcgttgaat acaaccagat cgcggcgcgc
     2941 gcgttccttg agcatcttgc gaagaaccgc cggcaatggc ttatcggtta cttcggtatc
     3001 aatgcccgga gctccagtcg aaccgagaaa ccctttggct ttgtcattcc ccgggagcag
     3061 cgtgacccgg gaacgacaga cgaactgctg gagattcttc agcgtggact tgtcgaggtg
     3121 atggaagcga cggcagagat cgagctcgat ggcgtggttt ggccggcagg cacacgtgtc
     3181 gtctccctca accagcccgc agggccgttt gccaagacgc ttctggaggt ccagaagtat
     3241 ccggatctgc ggcgttggcc agaagggcca ccctcgcatc cctacgacgt ggtaggacac
     3301 acattaccaa tccagatggg cgtgcgtgtc gtgcaggtcg acaagccgat cccggccgag
     3361 gcgccactga agctcgtcga ggagcaaatc cattacgagg gtgaggtcac ggagggcccc
     3421 tcaagtccac gggcgtgggt cttcgatgag cggtccaacg cctctatcgc tgccatcgcc
     3481 gaccttctgg tcgaggatgt gccggtgtac cgggccaaag aagcaatcgg cgagcttggg
     3541 attcgggccg ggtcaatcat cgttccggcc gacgagaaga accgcgcgct gatcaaggag
     3601 atcgcacagg gaacgggaac cgatgcacgg accatcgacg cgcctctgga catccctgcc
     3661 tggcggcaat ctcgcgtgcg gatgggtgtc tacaagccgt ggacagcttc gatggatgag
     3721 ggatgggcac gctgggtgct tgaggagttc gcaatcgagt atgagacgct ggaaacaccc
     3781 gacatccgtc aaggtaattt gcgagaccgg ttcgatgtga tcctgattcc aaatatgagc
     3841 cccgaacacc tcatggaagg gttgccggag aagaacaggt acaaggacct caatctcccc
     3901 gaatatgtcg gtggcatcgg tgcgctcggt atggatgagc tccggcgttt cgtggaacag
     3961 ggtggaacgc ttatcggggt tgatcaagga acccaggccc tgatcgaggg gatggcactc
     4021 cccgttcgta atgtattgcg tggcaagaag gagacggagt tctattgtcc gggatcactc
     4081 cttcgggtgg tgatgaatcc ggaccatgaa ctcggctacg ggctcccacg cgagcctgcg
     4141 gtgctcttta tggagagcgt tgctttcgag ggctttgggt ccgaggcgac atcggtcgct
     4201 acctatccaa ccacaaacgc caacctcagt ggctggatcc tgggcgagga gcacttgcag
     4261 cgcaagagcg cgctgatgga ggtgtcgtac ggggaaggat cggtggtgtt gctcggatt
//
LOCUS       DASYWM010000037         5695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_489187, whole genome shotgun sequence.
ACCESSION   DASYWM010000037 DASYWM010000000
VERSION     DASYWM010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489187"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VGR29_01445"
     CDS             complement(<1..941)
                     /locus_tag="VGR29_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV2072280.1"
                     /translation="MTPTQAIARRADLRNVAIIAHVDHGKTTLVDGLLKQSNIFRDPD
                     AAGDLILDANDLERERGITILSKNTAIQFHDVKINVIDTPGHADFGGEVERVLNMADG
                     CILLVDASEGPMPQTRTVLARALELGLRPIVVINKIDRSNARIDEVVELINDLFLDLA
                     IEDSQLDFPILYAIGRAGRAGFSPDDLAPDLLPLLETVLEIVPAPVADLEAPFQMLVS
                     SLDYDSHRGRIAIGRIHRGTIRAGEVLALTGENVDTVRQRVSSLMTFEGLGKRDVEEA
                     QAGDLIAVTGFNDAKIGSTITDPNHLDAIPAVAIEEPT"
     gene            complement(1335..3086)
                     /locus_tag="VGR29_01450"
     CDS             complement(1335..3086)
                     /locus_tag="VGR29_01450"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV2072281.1"
                     /translation="MSIREDSTAMRMAHLFGRTIREAPADAEFPSHQLMVRAAMIRPL
                     GAGIYSLLPLGYRVIRKVEQIVREEMDAIGGQEMLMPFVHPADVWRRSGRFDAIGPEM
                     VRLKDRGDRDMVLAMTHEEVVTDLTGTEISSYKQLPQIIYHLQSKFRDEPRARGGLIR
                     VREFTMKDSYSLDADEAGLDESYRQHAIAYTRIFERLGLDFIRVGADVGMMGGSASDE
                     YMAFSPHGEDVILICPNGDYAANREVAEFQADTPEEEELLPMEDVETPNAPTIQALAD
                     QLGVTLSRTAKAVFYKSGSGSFIFVVIRGDLDVNETKLRNAIGESDLTPATAEEIQAI
                     GAEPGYGSPLGVNDALIVADQSVARSPNLVAGANRPGWHVRNVNLGRDYSAEIVADIA
                     AAAEGALCPICGTPMQARRAIEVGNIFKLGTRYTTAMGATYLDQNGKAQPVVMGSYGI
                     GIGRSAASAVEQNYDDRGIIWPISIAPFHVSLLTIGKDDDVRAATDQIYADLQAAGIE
                     VLYDDRGERPGVKFNDADLIGNPVRLSISRRTLDNNEVEMKLRTESDAELVPLDGVVG
                     HVRGLVDRLIADLAPSA"
     gene            complement(3903..4448)
                     /locus_tag="VGR29_01455"
     CDS             complement(3903..4448)
                     /locus_tag="VGR29_01455"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEV2072282.1"
                     /translation="MKIYTALGDQGATDLLGERVSKDDPRIELMGSLDEATSSIGFGR
                     SIVTWQNANDLLISTQRDLYQIMAELAFIDTTRPDSLRFATQRVDWLEATIATVQEMV
                     ELPREFVVTGETTAGAALDVARTVVRRAERHAVALSKKGVVGNPEIIRYLNRLSTLLF
                     ILARAEDLQKVDHVRLAKGEA"
     gene            4719..>5695
                     /locus_tag="VGR29_01460"
     CDS             4719..>5695
                     /locus_tag="VGR29_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2072283.1"
                     /translation="MSTPLRARPAPALARSELSSRPIRVAVIGTGFGSTVHIPALAHV
                     DHTQLVAVCSRRQERASRVAAQYDIPTASTDWRDVINDPGVDAVVIATPPYLHHQMVI
                     TAIEAGKHVLCEKPLARNLAETRDMAKLAQSAQVVAMVNHQFRYLPIRMRVKELIDEG
                     YIGQPHAVSMIVFNSHLADPDGKRFGWLMESDKAGGMLRASGSHYIDSLRWWLGDIHA
                     VAGITGTMVKRRRLPDSTSMASVDADDNFAFLLRFASGALGSVHFSATAPTDAGESIT
                     ITGSEGMLIMEGDNELFGARKRDPGLRELAIPDRLNPKLPEFNHFLTRPT"
BASE COUNT         1135 a         1800 c         1633 g         1127 t
ORIGIN      
        1 gtaggctcct cgatcgcgac cgccggtatc gcgtcaagat gattcggatc cgtgatcgtg
       61 ctgccgatct tcgcatcgtt gaagccggta acagcgatga ggtcgccggc ctgggcttcc
      121 tcgacatcgc gcttgccaag accctcaaag gtcattagcg aggagacgcg ctgccgcacc
      181 gtatccacgt tctcgcccgt cagggccagg acctcgcccg cgcggattgt cccgcgatgg
      241 atgcgcccaa tagcgatccg gccccgatgg gagtcgtaat cgagcgacga gacaagcatc
      301 tggaaaggtg cctcgagatc ggccactggt gccggcacga tctcaagcac ggtttcgagg
      361 agcggcagca agtctggcgc aagatcatct ggcgagaacc cggctcgccc ggcgcgaccg
      421 atcgcgtaga gaatcggaaa atcgagctgg gaatcttcga tggcgagatc gaggaagaga
      481 tcgttgatga gctcaacaac ctcatcgatc cgcgcattgg agcgatcgat cttgttgatc
      541 acaacgatcg ggcgcaagcc aagctcgagc gcccgggcaa gcaccgtccg cgtctgcggc
      601 atcggtccct cggaagcgtc aacaagcagg atgcagccat cggccatgtt cagcacgcgc
      661 tcgacctcac caccgaaatc ggcgtgaccc ggcgtgtcga ttacattgat ctttacatca
      721 tgaaactgga tcgcggtatt tttcgaaaga atcgtgatgc cgcgctcacg ctccagatcg
      781 ttcgcgtcga ggatcaggtc gccggcggca tccgggtccc ggaagatatt ggactgcttg
      841 agaagtccgt cgacaagagt ggttttgccg tgatcaacat gcgcgatgat cgcaacgttc
      901 cgcagatccg cgcgacgggc gatagcctgg gttggtgtca tgggagtgtg cactttcggg
      961 tgatggcgcc cggtcgcagg aaacttgccg ggccacagaa gatgacccgt ccggttcggc
     1021 ccgagtcatt tcatgtgaag aatttcactt tcgtgctgaa ttatagcatg aatgccgcaa
     1081 aatcgctcct agggacccga acgaagcggc acttggcgtg aggcgacttg gtcggcacgc
     1141 ctggcccgag acaacgtgtg tcatatcagg actagctcga tggagcatgg atccaaggag
     1201 gctagcgaac gcttcagcat cccctctagc tgatcgggcg acgtacagtg tagggaacgc
     1261 atcttcccgt catagcactc ggttccccat gtggggaacc gagtgccggc gtcatccacc
     1321 ctgaacgact ttgcctatgc ggagggtgcc aggtcggcaa tcaaccggtc gacgagaccg
     1381 cgcacatggc ccacgacgcc gtccagggga acgagctcag catccgactc ggtgcgcaac
     1441 ttcatctcga cttcgttgtt gtccaatgtg cgtcggctga tcgagagccg aaccggatta
     1501 ccgatcaggt ctgcatcatt gaacttcacg cccggccgct cgccccggtc atcgtagagc
     1561 acctcgatac cagctgcctg cagatccgca tagatctgat ccgttgcggc acgaacatca
     1621 tcgtccttgc caatcgtcaa cagtgagaca tggaacgggg caatcgagat gggccagatg
     1681 atgccgcggt cgtcgtaatt ctgctcgaca gcagacgcgg ccgaacgtcc aatgccgatg
     1741 ccgtaggagc ccatcacaac cggctgggcc ttgccgttct ggtcgagata ggtcgccccc
     1801 atcgcggtcg tgtatcgagt gccaagcttg aagatgttac ccacctcgat ggcgcgtcga
     1861 gcctgcatcg gtgtaccgca tatgggacaa agcgcaccct cggccgctgc agcgatatct
     1921 gcgacgatct ccgccgagta gtcccgcccg aggttgacat tcctcacgtg ccagcccgga
     1981 cgattcgcac cagcaaccag gttcggggag cgggcaacgc tctggtcggc aacgatcaga
     2041 gcgtcgttca cgccaagcgg cgagccgtaa ccaggctccg cgccgatcgc ctggatctcc
     2101 tcggctgttg ccggggtcag atccgactcc ccgatggcgt tccgaagctt tgtctcattc
     2161 acatccagat caccccggat gacaacgaag ataaagctgc cactgccgga cttgtagaag
     2221 acggcctttg cggttcggct cagcgtgacg cccagttgat ctgccagcgc ctggatcgtc
     2281 ggcgcgttcg gcgtctcgac atcctccatc ggcaggagtt cttcctcctc tggtgtatcg
     2341 gcctggaact ccgccacctc ccggttcgcg gcgtaatccc cgttggggca gataagaatg
     2401 acgtcttcac cgtgcggcga gaacgccatg tattcatcgg atgcggagcc gcccatcatg
     2461 ccgacatcgg caccgacccg gataaaatcc aggcccagcc gctcgaagat gcgcgtgtag
     2521 gcgatcgcat gttgacgata cgactcgtcc agtccggcct cgtcggcatc gagcgagtac
     2581 gaatccttca tcgtgaactc acgcacccgg atgagcccgc ctcgtgctcg tggctcatca
     2641 cggaacttgg actgcaggtg gtagatgatc tgtggcaact gcttgtagga gctgatctcg
     2701 gtgccagtta ggtccgtgac gacctcctca tgggtcatcg ccagcaccat gtcccgatca
     2761 ccacggtcct tgagtcgcac catctccggt ccgatcgcgt cgaaccggcc agatcgccgc
     2821 cagacatccg ccggatggac gaacggcatg agcatctcct gtccaccgat cgcgtccatt
     2881 tcctcgcgga cgatctgctc aaccttgcgg ataacgcgat aacccaacgg cagcaggctg
     2941 tagatacccg cgcccaaggg acggatcatt gcggcgcgca ccatgagctg gtggctgggg
     3001 aactcggcat ccgccggagc ttcgcgaatt gtccgtccaa agagatgcgc catccgcatg
     3061 gctgtgctgt cctcccgaat gctcatgcat ccatcactca tgccctcgtt tcaacgagag
     3121 ggctcctgca tccgaaagaa tacaggttta catgggtatc cactcgcgta aagctgccaa
     3181 gggttagcac acaaggctgt ggccgtataa caagacttgg ggtggatgtg acggttgcta
     3241 gtgtatgcag tggataccgc ccagaagttg cccgacaaga gaatggcgtt gggatggaac
     3301 atgtggcacc cggtagacgg gccctgttgg cgaggattgg cccccgggac tcgaaggcgg
     3361 agttagccgt tcgacaagca gcacacgcac tggggtactg gttccgtcag caatcccacc
     3421 ggcccgtcat ggaatttcag ctcaggctga aacgtcgact cttcgctcca ggggcatggt
     3481 ctctcacggg atcgacgatg atcttgctat ccggtccgag gactggaacc tgtacccatc
     3541 gaacggaacg gtgggcgcgg caaagcgaac cacactgcca tcaccccgta tacacacaac
     3601 ttggccactt ggcagcctaa cctccgccgc tcccagccaa ggctgccagg tttccgtcag
     3661 gcgctctgga aacctggcca cctgaaaaat ccttgtcgta tgactgaaat ttgcccggat
     3721 tgattcctag atggccactg gccaagttaa ttacttggcc atctggagat ctttcgcaag
     3781 cgcagtaatc tccgcccttc gaccagggcc gacacttccc tcgccagcct ggtatcgatc
     3841 tcgagcacag acccggcgct tcgggcctgc tctgaccttg cggagtatcc tctcgactct
     3901 aactacgctt cgcctttcgc cagccgaacg tgatcgacct tctgcagatc ctcggcacgg
     3961 gcaaggatga agagcagcgt ggagagccgg ttgagatagc ggatgatctc cgggttcccc
     4021 acaacaccct ttttcgacaa tgccacggca tggcgctccg cacggcggac aaccgtgcgc
     4081 gcgacatcaa gcgcggcacc ggcggtcgtc tctccggtca ccacgaactc gcggggtagt
     4141 tccaccatct cctgaactgt cgcaatggtg gcttccagcc agtcaacgcg ttgggttgca
     4201 aaacgtaacg agtcaggtcg agtcgtatca ataaaggcca gctcggccat gatctggtag
     4261 aggtcgcgct gggtggaaat gagcaagtcg ttcgcgtttt gccacgtcac aatcgaccgg
     4321 ccgaacccta ttgacgacgt agcctcatcg agggatccca tcaactcaat gcgaggatcg
     4381 tccttgctga cccgctcacc cagaagatcg gtcgctccct gatccccgag tgccgtgtag
     4441 atcttcatcg gtcgtcatcc ttatttgtcc gcgcctgtta cgcggcatcg aattcaattt
     4501 ggtagcatgg atggccaggc ccgcgccggg attaccagca cactctcgaa acatggtacc
     4561 tgctggccat catcgggtcg tgccacgccg agcagtcaga ggcccgcttc atcactcctg
     4621 ccccgccatc tgcgatcggc tgcctcgacc acactcgccg cgtgcaccgg gtacttgcta
     4681 ctgccggttg tgcgttcgac cgagaatctg gagaacacat gtcgacaccg cttcgtgccc
     4741 gtcccgctcc cgcactcgct cgatccgaac tctcgagccg gcccatccgt gttgctgtca
     4801 tcggcaccgg gtttggttcc acggttcaca tcccggcact ggcgcacgtc gaccataccc
     4861 agcttgtcgc ggtctgctcc cgtcggcagg agcgcgcatc gcgtgtcgcc gcccagtacg
     4921 acatcccgac cgcgtcgacc gactggcggg acgtgatcaa tgacccggga gtcgatgcgg
     4981 ttgtcatcgc gaccccaccc tacctgcatc atcaaatggt gattaccgcg attgaggcag
     5041 gcaagcacgt gctctgcgag aaaccgctcg cccgcaatct tgcggagacg cgtgacatgg
     5101 cgaagctcgc ccagtcggcg caggtcgtcg cgatggtcaa ccaccagttt cgctatcttc
     5161 cgatccggat gcgcgtgaag gagctgatcg atgaggggta catcggtcaa ccacacgccg
     5221 tctccatgat cgtcttcaac tcccatctgg ctgatcctga cggcaaacgc ttcggatggc
     5281 ttatggaatc ggacaaggcc ggcggtatgc tccgcgcatc cggctcccat tacatcgact
     5341 ccctgcgatg gtggctgggc gacatccatg ccgtggccgg catcacagga acgatggtga
     5401 aacgccgccg gctcccggat tccacctcca tggccagcgt ggatgccgac gacaacttcg
     5461 ccttcctact tcgcttcgcc agcggagcgc tcggttctgt gcatttctcc gccacggcgc
     5521 cgactgatgc gggagaatcg atcaccatca ccggatcgga gggcatgctc atcatggaag
     5581 gcgataacga gcttttcggt gcccggaagc gcgaccctgg cttgcgtgag ctggctattc
     5641 ccgatcgcct caatccgaaa ctccccgagt tcaaccattt cctgacccga ccgac
//
LOCUS       DASYWM010000038         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_19973, whole genome shotgun sequence.
ACCESSION   DASYWM010000038 DASYWM010000000
VERSION     DASYWM010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19973"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VGR29_01465"
     CDS             <1..620
                     /locus_tag="VGR29_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV2072284.1"
                     /translation="EFGEFQHESDKNDAMRLVGAIHNATAQLSVDRLRKETLLVPKRT
                     ELQQALRALDVTWNAGPYSEPARRLLRDHAPVLHQRLGQFERLAAAVMGDRSGWVVSH
                     GEPHAGNIIRTRSGNMVVVDWDTAAYAPLERDLWMLLDEANPDWSAYRATTNVASLSH
                     RALGAYRLHWSLSEIAIYVSWCRSPHDRTEEMEIAWAGLQEYVMG"
     gene            complement(876..1130)
                     /locus_tag="VGR29_01470"
     CDS             complement(876..1130)
                     /locus_tag="VGR29_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072285.1"
                     /translation="MQPDPDSVPRHESVDHKHPTDAGMMNDDDEHTPGTGIMGDETLV
                     REVPGGLRLDSPSAGSRDCSSHPHPGTTPTPAMDQAEDKE"
     gene            1616..2197
                     /locus_tag="VGR29_01475"
     CDS             1616..2197
                     /locus_tag="VGR29_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072286.1"
                     /translation="MDGTRDVTDPRPDVPRRINVRGISGAGKSTFSAGLADRLGLTYI
                     ELDALHHGPNWSEPTIEEFRARVHAAMDAAAQGWVIDGSYDSKLGETVVGAADTIIWL
                     DLSLRTVFPRLWKRTMHRIRNNVELWNGNRETWRDQFASRQTIFFWTLRAHLRHRRDW
                     PSRFASDPRLVRLRSDAEVCRWLNALEQRVSKG"
BASE COUNT          494 a          742 c          869 g          553 t
ORIGIN      
        1 gcgagtttgg tgagttccag cacgagtctg acaagaacga cgccatgcgt ctggtcggtg
       61 caatccacaa cgcaactgcc cagctctcgg tcgatcggct gcgcaaggag acgttgctgg
      121 taccgaagcg gactgaacta cagcaggccc tgagggctct ggatgtcacc tggaatgcag
      181 gcccatattc cgaaccggct cgccgcctgc ttcgggatca cgcacctgtg ctgcatcagc
      241 gactgggtca attcgagcgg ttggctgctg cagtcatggg ggacagatcc ggctgggtcg
      301 tctcgcatgg ggagccgcac gccgggaata tcatccgtac gcgatccggg aatatggttg
      361 tcgtcgactg ggacaccgcg gcatacgcgc cgctcgagcg cgacctctgg atgctgctgg
      421 atgaggcgaa cccggactgg tccgcataca gggctacaac caacgtcgcg tctctttcgc
      481 atcgtgcgct gggtgcgtat cgactccatt ggagcctgtc cgagatcgcc atctacgtat
      541 cgtggtgccg tagcccgcat gaccgaacag aggagatgga gattgcctgg gcagggctgc
      601 aagagtatgt gatggggtag ggcttaccct gtgcgcgctt gctcaaggct accctggggc
      661 gagacggagc gatggggaat cccatacgac cactccagag cggcgtgggt gtctcgctat
      721 ctaaagatat gtatccggcg cggcaccgac atacggggac ctgacgtaat ccaggaaacc
      781 gggcctcggg aagggccggc tggcgataca ttggacgcgg cttgagcatc tcagtcacat
      841 ggcgctgttc gaggtgccag cgccagtcgg caacgttact ctttgtcttc cgcctgatcc
      901 atcgccggcg taggtgttgt gccggggtga gggtgggaag agcagtctcg tgatcctgcg
      961 gaaggagagt cgagacgcag gccgccgggg acttcgcgaa ccagggtttc atcacccatg
     1021 attccagtgc ccggggtgtg ttcgtcatcg tcgttcatca ttccggcgtc ggtcgggtgc
     1081 ttgtgatcta cggactcatg acgcgggacg ctatcggggt ctggctgcac aggattcctc
     1141 ctcaatcgag ggacgattgc cgtccccgag cgcgggtacg cacattttga tagacccaca
     1201 atacatcttc ggaaaccagg gggtgcggtg ctggcccagg gagcaggaga ggtgccactg
     1261 atgtacggag caacgtcgct cgtcgcgttg atccgggcag gtgcacggga gggatttctt
     1321 ggatgaccag cgacatcggc agttgcctgg tgaatcgacg ctactgtgca gggccatgag
     1381 atcacccagg ttacctgtat gctgaacctg cccctcggtc agcatcggat gcaatcaggt
     1441 ggtctccctt ctggcggatg gagaacatgg gaccgggaac agcacaagca cgggatgacg
     1501 aatcggctcg accggtgttc caacggaccg tggtcgtacc ggactggacg gacccaaagg
     1561 tctcgtgtgt ttgatgattg cttctctgtt gctcgccgcc acaaggagtc accttatgga
     1621 cggaacgcga gatgtgacgg atccaaggcc ggatgtcccg cgcaggatca atgtcagggg
     1681 tatatcaggt gccggcaagt ccacgttctc cgcgggtctt gccgaccggc ttgggctcac
     1741 ctatatcgag cttgacgcgc tgcaccatgg accaaattgg agcgagccga ccatcgagga
     1801 gttccgtgcc cgggtgcacg cagcgatgga cgctgccgcc caaggatggg taatcgacgg
     1861 cagttacgac tccaaactcg gggaaaccgt cgttggtgca gccgacacga tcatttggct
     1921 cgatctgtcg ttgaggacgg tattccctcg gctctggaag cggaccatgc accggatccg
     1981 caacaatgtc gagctctgga atggaaaccg tgagacatgg cgtgaccagt ttgccagccg
     2041 ccagacgatc tttttctgga ccctccgggc tcatctcagg caccggcgtg attggccgtc
     2101 tcgattcgcc tcggatcctc gccttgtccg cctccgttct gatgcggagg tgtgtcgttg
     2161 gctcaatgcg ctggagcaac gggtgtcaaa agggtagggg cttttggtgt tgattggcac
     2221 gataagaggc ggcgcccatg gattgaaggt ctggcttggc gatgagtctg aaagcacata
     2281 aaatccggtc tggatgcccc gaccgggttt cgtcctggtg ttactcccag ccttctgaag
     2341 gtgagctgct gaaggcgcga cctgcgtgtt caggccgcgc tttttgtagt catacccccg
     2401 gtcgatgtcg tccattgaga cgcccaaggc gggcgccaga tcgcgtaggt tggtcgcgga
     2461 tggttcattg cggccccgtt cccagttgta aacagtgctt ggtgcacctg gtggacggca
     2521 cgcactccgc agtctttacc tcgcagggtg tggtcaatgt ctcaggggat gtgccggcag
     2581 ccattgccga catcctgccg ccatcgttgc ctagtggttg gcgcggaagg cacgcgtcga
     2641 gggcgtgcag ctataccg
//
LOCUS       DASYWM010000039        26620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_245396, whole genome shotgun sequence.
ACCESSION   DASYWM010000039 DASYWM010000000
VERSION     DASYWM010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 26620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26620
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245396"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VGR29_01480"
     CDS             <1..738
                     /locus_tag="VGR29_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV2072287.1"
                     /translation="VVGADGRNSVVARQLGLDHALRWPHKTGLATHYRGVKGLDRFGE
                     MHIAPGIYAGLAILEDGLTNVTIVASVRDLESRSGSIEDFFSESIQRMPEVARKLESA
                     ERVGGIRGVGSMACRSRRTTGDGYLLIGDAAAFLDPFAGEGVYEALRGALLAAPVASA
                     AIRSGDVSADALDAYRIARRRTFTAKRSVSWIVQGFINTPPMMNYVTGRLARREELGL
                     TLSGVLGSFRPATQALSPLFLARLLRP"
     gene            757..1239
                     /locus_tag="VGR29_01485"
     CDS             757..1239
                     /locus_tag="VGR29_01485"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV2072288.1"
                     /translation="MRTENVVQMHGELDNIVALASDVERWPEILPHYRWVTLLEGGGD
                     RKVVEMAARRGRFPVKWRAVQEIERGNGTPVIRYHHIGGVTKGMDVAWTFDVSDGSVD
                     VRIDHDFSPPWPLVGGPIANGIIGPHFVEVIAGQTLATIKAIIEDRDPRFEPNGRARS
                     "
     gene            1236..2468
                     /locus_tag="VGR29_01490"
     CDS             1236..2468
                     /locus_tag="VGR29_01490"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV2072289.1"
                     /translation="MKQRVAITGIGAITPIGNGPEGLWAGVRGGDSAVREITRFDASS
                     FSCQVAAEIDFDPLDHLTAKQARRLDRFSQFALVSAKMALQDAGVTAEEAARVGAGIY
                     TGSALAGVAFAEEQHRSYVTKGPHHVNPLLALAVFGGAASSNVTIELGLTGPSLAYGN
                     SCAAGMIAIGEAARLIHDGRVPMMLAGGAEAPLAPLTFGAFTMIRVLSTRNDDPGTSS
                     RPFDQGRDGFVIAEGGAILVLESFEHATARGAHIYAELMGYGTSSDAHHMTAPHPDGI
                     HAARAMSDAISSAGLLPDDIEYVNAHGSSTVLNDSTECRAIRIALGGQAEKISVSGTK
                     GLHGHALGATGAMETAISALALERGHLPGTANLENQDPACDLDVIASGGRDTQVSHLL
                     CNAFGFGGINASLAMGAV"
     gene            complement(2576..4147)
                     /locus_tag="VGR29_01495"
     CDS             complement(2576..4147)
                     /locus_tag="VGR29_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV2072290.1"
                     /translation="MASSDREVTTDSGISIDPVYTAPDEPVELSAPGEYPYTRGIHPG
                     MYRSRRWTMRQYAGFSSAEESNKRYRLLLERGTTGLSVAFDLPTQLGMDSDDPRAFGE
                     VGRVGVAISTLDDMRLLFDQIDLQAVTTSMTINAPASLLLLMYQLVAEERGADARKLG
                     GTIQNDILKEYAARGTYIFPPRPSMRLVTDTFAYCAEHLPAWNTISVSGYHIREAGAS
                     AAQEIAFTIADGIAYIQAAVEAGMDVGAFAPRISFFFASHSNLFEEAAKFRAARRMWA
                     RILKERFGATNPKGMMLRFHTQTGGVTLTAQQPLNNVVRVAYQALSAVLGGTQSLHTN
                     GYDEALAIPTEEAATLALRTQQILAEETGVAETVDPLAGSYFVESLTDQLEAKAMEWL
                     ERIDEQGGAVAAIENGYMQNAIAENAYRRELARSSGDEIVIGVNRYVEDESTPMKLHR
                     IDPEMVARQIERVTTYKRNQDRTAVDATLEQVRQTAAGKENLLPVMKQALLQGATLGQ
                     VAYALRDVFGEHRTS"
     gene            complement(4134..4763)
                     /locus_tag="VGR29_01500"
     CDS             complement(4134..4763)
                     /locus_tag="VGR29_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2072291.1"
                     /translation="MKSLQDRLAFFVATDALKTTSRANWIYSEARTESVAEHVWHTTL
                     LAMLLADAAPDGIDHSHVRDLLTIHDLVEVYAGDTVLWDDVAIDDVDAREIAAGERLI
                     QMLPESQEERFDLLWREFQLQETSDARWARAIDALHPMVVSWFPGAPGHANKTLTPTR
                     ILTRKRPFLEPFPPLWSLAHWLVQQAVDQRLIPGDDLIGPTQETSSGII"
     gene            complement(5085..5501)
                     /gene="mce"
                     /locus_tag="VGR29_01505"
     CDS             complement(5085..5501)
                     /gene="mce"
                     /locus_tag="VGR29_01505"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEV2072292.1"
                     /translation="MSPESVLGELRLHHVGIVVADLDAAIARYEALGFRKGERHDIPE
                     QHIVAVTFRAGSGWVELIQPTALEGPIARYMAKRGEGTHHVAYAVDNIVAAMNRLRDA
                     GIRLIDETPRSGAHGWKIGFIHPEACHGVLTELVQE"
     gene            complement(5518..7938)
                     /locus_tag="VGR29_01510"
     CDS             complement(5518..7938)
                     /locus_tag="VGR29_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV2072293.1"
                     /translation="MARRRKRIPYDPPHKRKRRRGARAHLRADQFFLSRRTLVMKGGI
                     VAAFTALAAKLGVMQLQEGDRYKKAAAKNVIKEVTLPAARGLILDRQGRRLAQNRRAW
                     ELRVVPADLPKSKPERQRVLDTLISALQIEETLVVDPNALPDGSEDTVYSRIAAMLGF
                     EGPDVAELIEIWKSQATTEFLVNITSAGGLTIDDAARFRSARAELPGVQVMNRLNYLI
                     ENIWNPKLAVTVATDVPREIALKLESNRMYLPGVELDDSALVREYTGGQVMSHVVGYV
                     QNIPAALIDDPRWKGDKGERIYEQNDVIGRSGIELGMEQRLRGKRGRQSVERDASGLE
                     MRVLPGTIEEPDAGENIHLTIDLEFQEAVGKVLEQQIMAAAEGKRAENEKRRSEGKRE
                     WTIPKAGSAVAYDPRTGEILAMVSYPYYDNQLFINGISSRKWGEYVDRKAGAAFLNRA
                     TSELYPPGSTFKIFLATSALHRQTLNTTQTHVCRGGISVPNPYNLNDQLKMACWVAWN
                     NGTPHGELDVFGAIEQSCDVFFYNIAEERIKPRDAIDPIFYWDYDLNSNEILSDTKHV
                     FNGLGIEPIHEDMTTRFWFGKETGIEIEEIGGLFPGPEWKQEAIGEGWTVGDTLNISI
                     GQGEFTVTPLQIAMNTGILATRGAIRRPHLVRDTAGELATPVAATPEVVGDLGIAPEY
                     IDIVREGMRRVCHSETGTAFRDANGSKWKLTNPEGEVDQIIIAGKTGTAEFGFEDEIG
                     ARDSHALFTCFAPLDNPEIAVSVVIEAGGEGSTYAVPVADATLRAWFELNGRRPRGTV
                     LSPEPKPV"
     gene            complement(8073..8519)
                     /gene="mreD"
                     /locus_tag="VGR29_01515"
     CDS             complement(8073..8519)
                     /gene="mreD"
                     /locus_tag="VGR29_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV2072294.1"
                     /translation="MARLLFGLLLFATVFAQSTFLPRLNPFQVTPDLVLVLLFLWAAH
                     YSVREALLWVFITGIVIDVIAIDPLGANGLALVIVVVLSHPMRIRPWQFNLMSAMLLV
                     MLATVIHGVILYTLRGIPISSVIAIQAVVHALLVPLIYMSLRLIGR"
     gene            9053..10345
                     /locus_tag="VGR29_01520"
     CDS             9053..10345
                     /locus_tag="VGR29_01520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEV2072295.1"
                     /translation="MSAQETFIPRRVVVTGIGAISSLASNARETWNRVLNGETGIKRA
                     ANLDPAKHKCLLRSDVDDNTIPQRFLSGKALRNASRYARMAVEAAGEALMDAGLLNAD
                     LRPKIDLTDAGAALGNCIGGTYDDMIPAHDVAVERDSYARVPPHLHVTFPHNIGGYTI
                     QQRFDMHGPSSTVVTACATGSQAIGDGFHAIKFGRAPLMIAGAMESTQHPLFEAGFEA
                     MRALCTDSNDEPEKASRPFDATRAGFVLGEGAGMLVLEDLEHAQARGAHIYCEIAGYA
                     TSNDAYHPIAPQPEGFGAAKAIRDALNDAGVEPHEVDHVNAHAASTPAGDLAEAKAIK
                     LALGDRAEEIPVTSVKGAFGHSMAAAGALEAVMAVLTLHEGKIPPTRNYNNPDPEIGL
                     DIVGEARDAKIRVMTKHSFGLGGQNAVLVLKAWDEAIA"
     gene            10338..10742
                     /locus_tag="VGR29_01525"
     CDS             10338..10742
                     /locus_tag="VGR29_01525"
                     /inference="COORDINATES: protein motif:HMM:NF022279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2568 domain-containing protein"
                     /protein_id="HEV2072296.1"
                     /translation="MPDRASQSQPGPAEIGFFFLDIAALAGLARWGWHLGGGGLVGPA
                     LALLFVLLFGTVWAVFRARGFVPNGGDPVVAIPGPARLGIELLLYLLAAWSCWVSGWQ
                     VAAVVLVICAIAIYMAMRKRTIGLLRNTPPAA"
     gene            10857..11810
                     /gene="fabD"
                     /locus_tag="VGR29_01530"
     CDS             10857..11810
                     /gene="fabD"
                     /locus_tag="VGR29_01530"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV2072297.1"
                     /translation="MSLNETLGSTYGLIFPGQGSQAVGMGKNLADFSDAARQVMERAD
                     EILGFPLSTLCYEGPAEGLNDTYNSQAAIFTVSIAALHALEEEARQHDEYFVPMMVAG
                     HSLGQFSAMVAAEVIEFENALRLVRERGRLMKEAGTSHPGGMAAVIGMDDLKLAELVD
                     QAANGDVLTIANINCPGQTVISGELAPLDRFMDLAKDAGARKVARLPISIASHSSLMA
                     EASATLNDLLDSMTFHEPKMPLVANTTGKALNTAEEIREELRHHAERGVDWTGTIQTM
                     VDNGITTFGELGPGSVLAGLNRRIDRTTKTVGLKELGLPSA"
     gene            12056..13288
                     /gene="fabF"
                     /locus_tag="VGR29_01535"
     CDS             12056..13288
                     /gene="fabF"
                     /locus_tag="VGR29_01535"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV2072298.1"
                     /translation="MTRVVVTGVGAITPLGIGRDAFWSGLTEGRSGIRLIQNFDNSGL
                     EVKIAGEVPDFRAKDFMDFKAARRMDRFSQFGVAAAREAIEHAGIEITDDNRDRIGIM
                     INTGGGGIPTIEHEVTVMAKSGGKRVSPFLIPLFAPNMASCQVAIQFNIHGPTVTSVA
                     ACAAGVQAFVDAVHMIERGDADVVITGGTEGGITPVAIASLTNMGALSRRNDDPQRAS
                     RPFDTARDGFVFSEGAAVVILESEEHAARRGANVLCQASGGAYTSDAFHITAPDPNGR
                     GATTAMKRALQRAELDPTDVDYVAAHATATQIGDIAETTSIKNVFGDHAYKLSISANK
                     SMLGHLLGGAGAVSSLACILAIRDNIVPPTINLDNQDPQCDLDYTPNVARKAEIKTAL
                     ANGFGFGGQNAVTVFRQI"
     gene            13432..14163
                     /locus_tag="VGR29_01540"
     CDS             13432..14163
                     /locus_tag="VGR29_01540"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2072299.1"
                     /translation="MAHDVALVTGSAKGIGYAIARRLLRDGMTVIVADIDSRGAESSA
                     AELGDRAHALHLDVTSHERWESGVTSVARQHGTVSVLVNCAGIAGRSAPCWELSPEEW
                     HQVIGIDLTGVFFGCRAVLPAMIDATYGRIVNIASIAGKEGNPNASPYSAAKSGVLGL
                     TKSIAKEVAAKGILVNAVTPAVIETEILKQVTPEHIAYMTSRIPMGRVGQPEEVAALV
                     SFLCSRELSFSTGAVFDISGGRATY"
     gene            14414..14839
                     /locus_tag="VGR29_01545"
     CDS             14414..14839
                     /locus_tag="VGR29_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072300.1"
                     /translation="MMVEPRPEISGHQFTLKGSPRHYDDVEKIIEQGGGATVYFGEAL
                     TYERGAGVAQWRVKAKSLDWLPSLYDWWAEAERVEPINFTFHLYHPENLGYPVLDLSQ
                     HTAEEVAEYIADNMPWVGSDPVTTKPSRMSSGEPTMNAH"
     gene            complement(14996..15571)
                     /locus_tag="VGR29_01550"
     CDS             complement(14996..15571)
                     /locus_tag="VGR29_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072301.1"
                     /translation="MSNSNAETPGGQKNRSSRPRRRSRKRQSGGGAPRREAQPQEPIQ
                     PLPVAENRAPQPQQRDGAGRTNRRRGGRRKPTEPIPGEVIRRDAEADAPIQPMRKRPL
                     SEAEDTTEPTFGCPMLTRTRLGMPFRGGQHVPRCSVGWAVHDEDEVLLCMHTPTRNQC
                     WKEHPEHLEDLIETLRPLIEAELKGEETAAD"
     gene            complement(15522..16112)
                     /locus_tag="VGR29_01555"
     CDS             complement(15522..16112)
                     /locus_tag="VGR29_01555"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEV2072302.1"
                     /translation="MDEASQGRQPVQGTPDVNIPGRSTNERTPGILPQEQPDEPIDGT
                     SSDAMQRFWEAVKRLPAYIKLVARIARDPDVPKQAKTVLAVGGGYAVSPIDLVPGVIP
                     VAGQIDDMYVLLTAIQKSLQMMPTEIANRNLALAHVSREEIDADLQAVRDVVRMAVKK
                     SIKVGGKALERVSRAAFGFAREQLKRRNAGRSEKPL"
     gene            complement(16340..17260)
                     /locus_tag="VGR29_01560"
     CDS             complement(16340..17260)
                     /locus_tag="VGR29_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEV2072303.1"
                     /translation="MNLMRRAVLGVADLPPVHRLFVRNRYARQLVNRFVAGDTLESAL
                     TVARELAGEGITVTLDLLGENVSTRDAAAAAVGSYTETLRQMKANDLEPNISVKLTML
                     GLDLGDDVARENMTRILEAAREVGGFVRIDMEGSDYTTRTMQIFGQLHDRFPEEVGIV
                     IQSYLHRAASDVVDMINRGARVRLVKGAYAEPESVAIQSRTENDENYERLMQHLLDKG
                     NYPAIATHDPALIEATIAYARANTIPPDRYEFQMLYGVRRNEQLRLKRAGYGMRVYVP
                     FGTEWYPYFTRRIAERPSNAFFVLRQVFDR"
     gene            complement(17378..18709)
                     /locus_tag="VGR29_01565"
     CDS             complement(17378..18709)
                     /locus_tag="VGR29_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072304.1"
                     /translation="MSVDQDNPMAARAAAILRANDTGTSIKAAPALYPHQWSWDAAIV
                     SIGLVHLSLDRAIQEMRTLFRGQWKNGMLPHIVYSADVPKDAYFPDADRWDVAHVSPD
                     VPEGIATSGIIQPPIHAIAIRRICDQANPEQARAIASEFYGPLLEWHRYLVSARDPEV
                     SGLITIVHPWESGMDNSPRWDQAMARIQVPEGGVPPFERRDLSHVSDASQRPTNEDYE
                     RFIWIVEVLKRGSYRVDQIYDELPFRVKDIFMSAMLVAANEALIRIAALADGDFDDLT
                     IIESWIDRGRRGLAEQWLPDEGLCCDLDVIDNSWIKVQTIATFAPLLAGQLTADTLTA
                     LNRVWSSEAFTGHPELRWALPPSTSPGEPSFVPNRYWRGPVWPVMNWLLWWAWERIGE
                     GEIAAALRGNALDQISSAGFAEYVEPFTGEALGSDDQSWTAAVVLDWLAAS"
     gene            18847..20217
                     /locus_tag="VGR29_01570"
     CDS             18847..20217
                     /locus_tag="VGR29_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2072305.1"
                     /translation="MIEDILEYRDVIGTSPAPQERYTSVIRDGLYDRIPMIAPAIALI
                     STLPFLRLDRIQQLGFVSRVWPGAKHTRYEHSLGVMHLTRLAVEHLCSTPEGTWITPE
                     DARTVVAAALLHDIGHYPFSHAIEEIGAPVATHEAVGQVIIESGEIAEILSQDWLVDP
                     ARVAGIIHDDRSLLNEADLVLGGLLSGTLDMDKLDYLPRDARACNVPYGGVDTARLIE
                     SLTIVEVDLPGTRTSQRIAITEKGISPLHSLINARQEMFDNVYWHHTNRACMVMLLRA
                     IQDALLADAISPGSLTGHDDASLLAFMRSVDMPAATRRLADALAGRRIHKRAVEISAR
                     AESLYSRLAQLWYDQRARREVELRLVAALSNTLGEPVEDTAILIDIPKPEKWKTDVWV
                     SFRQPPVGMQPLMHWQDVVGLGDEDFKRYEEHRRMIRIVTEEPYRHVLAETWEALLYP
                     ALGGAL"
     gene            20232..20456
                     /locus_tag="VGR29_01575"
     CDS             20232..20456
                     /locus_tag="VGR29_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072306.1"
                     /translation="MTDQQQLADLQTPEPSQAETIIRPARPDDTVQSDRERADTDLPE
                     NDPHGTVSIVLMGVLMLMLVLLVLVIVQAA"
     gene            complement(20489..21388)
                     /locus_tag="VGR29_01580"
     CDS             complement(20489..21388)
                     /locus_tag="VGR29_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2072307.1"
                     /translation="MMAKTIVVTGASSGFGKGVAQRLAEQGHNLVLAARRGELLEELA
                     REIGHAIAVPTDVANPDEVHYLGERAKAEYGRIDVWINDAGVGTIGRFTDLPLRDQIS
                     VVETNLIGALNGTYVAMQQFVEHGEGTLINVASVAGKVAMPYFAVYGGTKSAILSMSA
                     SIRRELELNGQDNIHVCVVNPWATDTSFFEHGSNYTRHSLRMPMIDDPEIVVDAIVGL
                     IDDPKDEVDVGVKSKGTALGSHLAPGLTEAASAKLIHHTLMEEAPLAGVTSGSVNEPV
                     AAGTEVEGHTRERIAEEDRRKES"
     gene            complement(21486..23207)
                     /gene="ilvD"
                     /locus_tag="VGR29_01585"
     CDS             complement(21486..23207)
                     /gene="ilvD"
                     /locus_tag="VGR29_01585"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV2072308.1"
                     /translation="MSTDGKANGSNGQSEQVDMRFNSRTLYDGNDRAGARSMVKAIGF
                     TDEDLQKPIIGIANTWIETMPCNFGLRRLAEQVKAGVRAAGGTPMEFNTVAISDGVTM
                     GTEGMKTSLISREVIADSVELVGRGHMFDGIIALGACDKTLPGLSIALARLNVPGFIL
                     YGGTIMPGYWQGKEVTIQTVYEAIGANAAGKMSDEDLKDLENHACPGAGACGGQFTAN
                     TMAMAMEFIGLSPLGSASPPAIDPRKEEVGLKAGELIIDMLRRNQRPLDILTRGAFEN
                     AIAGVAASGGSTNSVLHLLALAREAGVPLTIEDFDVISRRTPLITDLMPSGKYAAADL
                     DAAGGTQLVAQRLVDAGYIDGSQATPTGRSLADEAATATETAGQDVVHAFDNPMKATG
                     GLNILKGNLAPDGCVVKLFGYEPMHLTGPARVFNSADEAGDAVLHNRIVPGDVVVIRY
                     EGPKGGPGMQEMLGITAAIVGQGLGGSVALITDGRFSGATRGLMIGHVAPEAFVGGPI
                     AALQEGDLIEIDIENRALNVHLSEDEIATRLRDWAQPEPRYKQGVLAKYAAHVQQASE
                     GAVTMLQ"
     gene            23463..24341
                     /locus_tag="VGR29_01590"
     CDS             23463..24341
                     /locus_tag="VGR29_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2072309.1"
                     /translation="MTDRGVREFGMQTIQYWTWEETRERWIWFEDLGFESLWLTDHFV
                     RTAQPESPYFEAWTLLSALAGVTRNARLGVLVSSNTFRHPSLLAKQAVTLDHISGGRL
                     ELGIGAGWFQPEHEMFGLEFPPTGELVDRYEEAIDLLDRYLRNDITSFEGAYYSLKHA
                     PNRPTPVQQPRIPFVLGAHKPRMIRLAARYADTWNSRGTPGEIRDRNDLLDESCVEEG
                     RDPKEIKRSLLYVIAQMTDERPWDSIDAFEDFVGRFTEAGMQEFIFQPPDPEHYAIVE
                     RIATDVMPRLRGSDTA"
     gene            24499..24747
                     /locus_tag="VGR29_01595"
     CDS             24499..24747
                     /locus_tag="VGR29_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV2072310.1"
                     /translation="MGITELLLNILSIYSFVLFGRAIMSWFDPGFRSTIGRILYDITE
                     PVVAPVRQVVPSLGGLDLSIMVTLFLILILQRLLSSAL"
     gene            24922..25143
                     /locus_tag="VGR29_01600"
     CDS             24922..25143
                     /locus_tag="VGR29_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072311.1"
                     /translation="MLLRLLFLISLLGYATGPDAREREEDDKLGGEGFGTIVMLFVGL
                     GLLLLLALVVLAILAVTGQFDSEFMDPIT"
     gene            complement(25201..>26620)
                     /locus_tag="VGR29_01605"
     CDS             complement(25201..>26620)
                     /locus_tag="VGR29_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HEV2072312.1"
                     /translation="RIAIPATRYGWDALYRSVLTTSSLDDLAVRQEAIAELAGEVDVQ
                     QAVEAAALRDDEPLPDPQALVHWAGSTPWLRPRICLRAVSWVSPVVFVILLALWALGV
                     TSSPLFVIPVALNGLIFALWGQQAAVRVQAIVPLRDAIAGYRDIFWTIATARSRASLL
                     RHIDDTLEGGHDGALARTAQLARAVSLAIPPGAIIYFPLQLAFLWDIHVLELLEGWQA
                     KAGDRVHAWLRAAGEWEALASFSILHRDHPGWAFPRVTPEAVSLFAAELSHPLLREQE
                     AVANDVTVGPEAHFLFVTGSNMSGKSTLLRAIGANVVLAQAGAPVFARSLSMPPVRLW
                     TCMRVEDSLEQGVSFFMAELQRLKAVVDGVASDPSRTALYLLDEILQGTNTAERQIAP
                     RRVLASLVEQRAFGAISSHDLELIEGSTLKDSATLVHFAEVFLPGPDLPTMTFDYRLR
                     PGLATSSNALALMEMLGFDLKT"
BASE COUNT         5303 a         8354 c         7679 g         5284 t
ORIGIN      
        1 gtcgttggcg cggacgggcg caactcggtc gttgcgcgcc aactggggct cgaccacgcc
       61 ctgcgctggc cccacaaaac cggtctcgcg acgcattatc gcggtgtaaa ggggcttgat
      121 cgcttcggcg agatgcatat cgctcctggc atttacgcag ggctggcaat tctcgaagac
      181 gggctcacaa acgtgacgat cgtcgccagt gtgcgggacc tggaaagccg gtccggatcg
      241 atcgaggact tcttctcgga gtcgatccag cgcatgccgg aggtagcgag aaaactggaa
      301 agcgcagagc gggttggcgg aattcgtggt gttggatcca tggcctgccg tagtcgccgt
      361 accaccggtg atggatacct gctcattgga gacgccgccg cgttccttga tccctttgcc
      421 ggggagggcg tctatgaagc gttgcgaggt gcgttgcttg ccgctcccgt tgccagtgcc
      481 gcgattcggt ccggggatgt ctccgccgac gctctagacg cctaccgcat tgctcgccgt
      541 cgcaccttca ccgccaagcg ttcagtgagc tggatcgtgc agggatttat caacactccc
      601 ccgatgatga actatgtaac tgggcgactg gcgcgtcgtg aagagctcgg tctgaccctc
      661 agtggcgtac tcggcagttt ccgtcctgcc actcaggccc tttccccgct tttcctggcc
      721 cgattgttgc gtccctgatg acaaaaggag acatgaatgc ggacagagaa cgtcgttcag
      781 atgcatggcg aactggacaa tatcgtcgcc ctcgcatccg atgtcgagcg ttggccagag
      841 atccttcctc attaccggtg ggtgacactg ttggagggtg gcggcgatcg caaggtggtt
      901 gagatggcgg ctcggcgggg tcgatttccc gtcaagtggc gtgcggtcca ggagatcgag
      961 cggggcaatg gcaccccggt cattcgctat caccatatcg gtggcgttac gaaaggcatg
     1021 gatgttgcct ggactttcga cgtcagcgat ggatcagtcg atgtaaggat tgaccacgat
     1081 ttctcgccgc catggccgct tgtcggcgga ccaatcgcca atggaattat cggccctcac
     1141 tttgttgagg tgatcgcggg ccagacgctc gccacgatca aggcgatcat cgaggatcgg
     1201 gacccgcgtt tcgagcccaa tgggagagca cgatcttgaa gcaacgcgtc gcaatcacgg
     1261 gcattggtgc cattacgccc attgggaacg gtcccgaggg actctgggcg ggcgtgcgcg
     1321 gtggagactc cgccgtccgc gagattacgc gcttcgatgc ctcctcgttc tcctgccagg
     1381 tggcggcgga gattgatttt gacccgctgg accatctgac agccaagcag gcgcgccggc
     1441 tcgaccggtt ttcccaattt gccctcgtct ccgcgaagat ggcgttgcag gatgcgggcg
     1501 tgacggcaga ggaagcagcg cgggttggtg ccggcatcta caccgggtcc gcgctcgcgg
     1561 gagtcgcctt cgcggaggag cagcacagat cctacgtgac caagggcccg catcacgtga
     1621 acccgctcct cgcgctcgcc gtcttcggtg gcgcggcctc gtccaatgtg accatcgagc
     1681 tcggcctcac cgggccctcc ctggcctacg ggaactcctg tgcggccggg atgatcgcga
     1741 tcggtgaggc agcaaggctc atccacgacg gacgggttcc catgatgctg gcgggcggcg
     1801 ccgaggcacc gctcgctccc ctcaccttcg gcgcattcac catgatccgt gtgttgagca
     1861 cccgcaacga tgaccccgga acctcaagcc gaccattcga tcagggacgc gatggctttg
     1921 tcattgccga gggaggagca atcctggtcc tggaatcatt cgagcacgcg acggcacgtg
     1981 gcgcgcacat ctatgccgag ctcatgggat acgggacttc cagtgatgcg caccacatga
     2041 ccgcccccca tccggacggg attcacgcgg cacgggcgat gagtgacgcc atttccagcg
     2101 cgggccttct gccagacgac atcgagtatg tgaacgcgca cggatcatcg acggtactca
     2161 atgactcgac cgagtgtcgt gccatccgta ttgctctggg cggccaggcg gagaagatat
     2221 cggtgagcgg tacaaagggg ttgcacggtc acgcgctcgg tgccaccggc gccatggaaa
     2281 cagcaatctc cgcacttgca ctggagcgtg ggcaccttcc cggcaccgcc aaccttgaga
     2341 atcaggatcc tgcctgcgat ctcgatgtga tcgcctcagg tggtcgcgat acgcaggtat
     2401 cgcacctgct gtgcaacgca ttcggcttcg gcggcattaa tgccagcctt gccatgggcg
     2461 cggtgtaata gcctccaaac attccaccac gtcggcagga caggaataac ctgctttagt
     2521 gtcgacactc tgctctaaat attgatgcgg taaggagagc tgttgcaggg ctctcctacg
     2581 atgtccgatg ctcgccaaag acatcccgta gggcgtaggc aacctgacca agcgtcgcgc
     2641 cttgcagcaa agcctgtttc attaccggga gcagattctc tttaccagcc gcagtctgtc
     2701 gtacctgctc cagagtcgca tccaccgccg tgcggtcctg gttccgcttg taagtcgtca
     2761 cccgctcgat ctgtcgcgcc accatctccg gatcgatcct gtgcagcttc attggcgtcg
     2821 attcgtcctc gacataccga ttcacgccaa tgacaatctc atcaccggag gaccgggcaa
     2881 gctcgcgacg ataggcgttc tcggcaatcg cgttctgcat gtagccgttc tcaatcgcgg
     2941 cgaccgcccc accctgctca tcgatgcgct ccagccattc catcgccttt gcttccagct
     3001 gatcagtcaa cgattcaacg aaataggacc cggccagcgg atcgacggtc tcggcaacac
     3061 cagtctcctc ggccagaatc tgctgtgtcc ggagggcgag cgttgccgcc tcctcggtcg
     3121 gtattgccag tgcctcatcg tagccattcg tatgcaggct ctgggtgccg cccagcactg
     3181 cggaaagcgc ctgatacgcg acccgaacaa cattgttcaa cggctgctgg gccgtcaggg
     3241 tgacgccccc cgtctgcgta tggaagcgca gcatcatccc cttcgggttt gtcgccccga
     3301 aacgctcttt caggatcctt gcccacattc gccgcgcggc ccggaacttc gcggcctcct
     3361 cgaaaaggtt ggaatgcgaa gcgaagaaaa agctgatccg tggtgcaaat gcacctacat
     3421 ccatgccggc ctcaaccgca gcctggatat aggcgatgcc gtccgcaatc gtgaacgcaa
     3481 tctcctgggc ggcagatgct ccagcctcgc ggatgtgata accagagacg gagatcgtgt
     3541 tccacgccgg caggtgctcg gcgcagtacg caaacgtatc ggtgacgagg cgcatcgatg
     3601 ggcgcggcgg gaagatatag gttccccggg ccgcatattc cttcaggatg tcgttctgga
     3661 ttgtcccgcc gagcttgcgg gcgtcagcac ctcgctcctc cgccacgagc tggtacatca
     3721 gcagcagcag agaggcaggc gcgttgatcg tcatcgaggt ggtgacagcc tgcagatcga
     3781 tctggtcgaa gagcagccgc atatcgtcca gcgtgctaat ggcgacgccc actcgaccga
     3841 cttcaccgaa ggcacgtgga tcatcggaat ccataccgag ctgtgtcgga aggtcgaacg
     3901 cgacactcag gccggtagta ccccgctcca gcaagaggcg atagcgcttg ttgctctctt
     3961 cagccgagga gaacccggca tattggcgca tcgtccatcg ccgggaccgg tacatacccg
     4021 gatggatgcc acgggtatac gggtactcac caggggcgga gagctccacg ggttcatccg
     4081 gagcggtata gacgggatcg atgctgatac cggaatcggt agtgacttcg cgatcagatg
     4141 atgccactcg acgtctcctg cgttggtcca atcaggtcat cgcccgggat cagccgctga
     4201 tccaccgcct gctgcaccag ccagtgcgca aggctccata gcggcgggaa gggctccaga
     4261 aaggggcgct tgcgtgtcag gatccttgtt ggtgtgaggg tcttgttggc atgtcccggt
     4321 gcaccgggga accagctcac caccataggg tgcagcgcgt cgatggcacg tgcccagcgc
     4381 gcgtcggacg tctcctgcaa ctggaactcg cgccacagca gatcaaacct ctcctcctgc
     4441 gactccggaa gcatctggat cagacgctcc cccgcggcga tctctcttgc atcgacatca
     4501 tcgatggcga catcgtccca caacaccgtg tcgccagcat agacctcaac caggtcgtgg
     4561 atcgtcagca gatcacgaac atgactgtgg tcgatgccat cgggtgctgc atcggccagg
     4621 agcatcgcca gcaatgtcgt atgccagaca tgctccgcca cactttcggt gcgcgcttcg
     4681 gaatagatcc agttcgcgcg ggatgtcgtc ttcagtgcat ccgtagcaac gaagaaggcg
     4741 agtcggtcct gcaacgattt cacctggtct cctgatcttc catgcttgac gtgggcatag
     4801 agctactcac cctccccatc ctacctgttt ggtgccgaca aagaactcgg tatcctgaca
     4861 tcaggaagaa ttccgctccg cgccaagcgg tgtctcaact ggaccgctgg acatcggtgc
     4921 gcataatcga cctccgtcac tgaggcatgc tgcttcgcag acgcattctc acgcggcgca
     4981 aggagatgac accggagttg ttttctatgg ccgggaacga ggcaatggac aagcctcagc
     5041 catcgctcgg gataggagct cgatccatca aggcaagtga ccacctactc ctgcaccagc
     5101 tcggtgagga cgccgtgaca tgcttcgggg tggatgaacc cgatcttcca tccgtgggcg
     5161 ccagagcgtg gcgtctcatc aatcaggcga atgcccgcgt cgcgcagccg gttcatcgct
     5221 gcgacgatat tatccaccgc gtaggcgacg tggtgcgttc cttcgccgcg ctttgccata
     5281 taccgcgcga ttggtccctc taaagcggtc ggctggataa gctccaccca tcccgaacca
     5341 gccctgaatg taacagcgac gatgtgttgc tcggggatgt cgtgtcgctc tcccttgcga
     5401 aaccccagcg cctcataccg ggcaatggca gcatcgagat cagcgacgac aataccgaca
     5461 tgatgcagtc gcaactcacc aaggaccgat tcaggactca tcacgctaag gacgctccta
     5521 tacgggtttc ggctccggac tcagcaccgt accgcgtggc cggcgcccat tgagttcgaa
     5581 ccaggcccgc aatgtcgcgt cggcaaccgg cacggcgtag gtggagccct cgccaccggc
     5641 ctcaattaca accgacacgg cgatctccgg attatcgagt ggcgcaaagc acgtgaacaa
     5701 ggcatgtgaa tcccgggcac cgatctcgtc ttcgaagcca aactcggcgg taccggtctt
     5761 tccggcaatg atgatctggt cgacttcacc ctcagggttg gtcagcttcc attttgatcc
     5821 gttggcatcc cggaaggctg tccctgtttc tgaatgacaa actcgccgca taccttcccg
     5881 aacgatgtcg atgtattcgg gcgcgatgcc cagatccccg acaacttcag gtgtggccgc
     5941 aactggcgta gctaactcac ccgcggtgtc acgaacgaga tgtggcctcc ggatggcacc
     6001 ccttgtggca aggataccgg tgttcatcgc gatctggagc ggtgtcacgg tgaactcgcc
     6061 ctgaccgatc gagatattga gggtgtctcc gacggtccac ccctcaccga tcgcctcctg
     6121 tttccactca ggccccggga aaagcccccc gatctcctcg atctcgatgc cggtttcctt
     6181 gccgaaccag aacctggtgg tcatgtcctc gtggattggt tcgataccta gaccgttgaa
     6241 gacgtgcttc gtatcactca ggatttcgtt gctgttcagg tcgtaatccc aatagaagat
     6301 ggggtcgatg gcgtcccgag gtttgatcct ctcttctgcg atgttgtaga agaagacatc
     6361 acacgactgc tcaattgcac caaagacgtc cagttctccg tgaggggtac cgttgttcca
     6421 ggccacccag cacgccatct tcagctggtc gttgagattg tagggattgg gaacgctgat
     6481 gcctccacga caaacgtgcg tttgggtggt attgagcgtc tgccgatgca gtgcggacgt
     6541 ggccaggaag atcttgaagg tcgatcccgg gggatagagc tcgctggttg cccggttgag
     6601 gaacgcggca ccggccttcc tgtccacgta ctcgccccac ttccgggagg agattccgtt
     6661 gatgaagagc tggttgtcgt agtagggata gctcaccatg gcaaggattt caccggttcg
     6721 cggatcgtag gcaacggcac tccccgcttt cgggatggtc cactcccgct tgccctcgct
     6781 ccgccgcttc tcattttcgg cacgcttgcc ttctgccgca gccataatct gctgctcgag
     6841 taccttcccc acagcctcct ggaactccag gtcaatagtg aggtggatat tctcgccggc
     6901 atccggttcc tcaatagttc ctggcagtac ccgcatctcc agcccactcg catcgcgctc
     6961 cacagactgc cggccccgct tgccccgaag ccgctgctcc atgccgagct cgataccgga
     7021 tcgcccaatc acatcgtttt gctcgtagat gcgctctccc ttgtccccct tccagcgagg
     7081 atcgtcgatc agcgccgcgg ggatgttctg aacgtacccg accacgtggg acatgacctg
     7141 accaccggtg tactcgcgga ccaaggcgga gtcgtcgagt tcgacgcccg gcaggtacat
     7201 cctgttggat tcgagcttga gggcgatctc gcgaggaaca tccgtggcga ccgtcaccgc
     7261 cagtttcggg ttccagatgt tctcgatcag gtagttaagg cgattcataa cctgcacgcc
     7321 tggcagctct gcgcgcgccg aacggaatcg ggcagcatca tcgatcgtca accctcccgc
     7381 cgacgtgata ttcaccagga attcagtcgt ggcctgggat ttccatattt caatcaactc
     7441 ggcaacgtcg gggccctcaa agccgagcat tgccgcgatt cgactgtaga cggtgtcctc
     7501 tgaaccgtcc ggcaaggcgt tcggatccac gaccagcgtt tcctcgattt gcagtgcgga
     7561 tatcagcgta tcgagaacgc gctgccgttc gggcttgctc ttgggtaggt cggccgggac
     7621 tacccgaagc tcccaggcgc gtcgattctg ggcaaggcga cgtccctggc gatcaagaat
     7681 caacccacgc gccgcaggca acgtcacctc cttgatgacg tttttcgcgg ctgctttctt
     7741 gtagcgatcg ccttcctgca actgcatgac accaagcttc gcggccaggg ctgtaaaggc
     7801 ggccacaatc ccacccttca tcacaagggt gcgccggctg agaaagaact gatcagcgcg
     7861 caggtgagcg cgcgcgccac ggcgccgctt gcgcttgtgt ggaggatcgt agggaatgcg
     7921 ttttcttcgt cgtgccatag gttagcctct gggcgaacac tcaggtgcgg agcttgttcg
     7981 atcgttggat ggggagcgta ttgccgtagc agtctctggt agtgcggctc ggtgtctgcc
     8041 tgctcacccg acgcctgtta aagggtacgt cgtcagcgcc caatcagacg caagctcatg
     8101 tagatgagag gaacgagcaa cgcatgaaca accgcctgga ttgcgatgac agaggaaata
     8161 ggaatacccc gtaacgtgta caaaatgacg ccgtgaatga cagttgccag cataaccagc
     8221 aacatggcac tcatcagatt gaactgccat ggtcggatcc gcatcgggtg tgagagcacg
     8281 accacaatga ccagtgcgag cccgttggcc ccgagtggat caatggcaat aacatcgatc
     8341 acgatcccgg tgatgaacac ccagagcaac gcttcgcgca cgctgtagtg cgcggcccac
     8401 aagaaaagca ggaccaacac caaatccggc gtcacttgga acggattgag cctggggaga
     8461 aacgttgact gggcaaacac cgtcgcgaac aaaagaagcc cgaagagcaa gcgcgccata
     8521 agttcgtcag gtgtgctccc tcgtcaatcc ggcgtcactg tcccacagga cgtccccggc
     8581 tcgtgctacc gattcagtct agcatcatgg tggggagagt ctgttgcagg aaccacgaat
     8641 cgggcaagaa ccctatcatt gacgaagaga ctcacagatg taaatacacg gccgctggcc
     8701 tctatccacg aagcattctt tgcgggtggc aaattccctt tcaaccaccc gcgagcccga
     8761 tgtgcttggc gttccaggtc agcggctaaa ggcggttttc gacagcttgg acattatcca
     8821 gcgcagggct gccaatattg ccccgatgga tcacgggaac atgcatcaat cccggcgctc
     8881 ggctcgtgca tcacggcatg ctacccacat gcccaatcgc tgcagaagca tgctgatctg
     8941 ccgtatgatc cgcagatgat attcgcatcg cctgtcggtc cacgcttgca gcgtcagaca
     9001 ggcaattctc gaacagatat cgtgaattcc aggagtccag aaaggtgctc ggatgtccgc
     9061 tcaagagacg ttcattccac gccgtgtcgt tgtaactggt atcggcgcca tctcctcact
     9121 cgcgagcaac gccagggaaa cctggaaccg tgtgctcaat ggggaaaccg gcatcaagcg
     9181 ggccgccaat ctggatcccg ccaaacacaa gtgcctgctg cggagtgatg tcgacgataa
     9241 cactatcccg cagaggttcc tcagcggcaa agccctgcgc aatgcctccc gctacgcgag
     9301 aatggctgtt gaggcagccg gcgaggcgct gatggacgcc ggcctcctta atgccgatct
     9361 acggccaaag atcgatttaa cggacgccgg agcggcgctc ggaaactgca tcggcggaac
     9421 ctacgacgac atgattccgg cccatgatgt cgccgtggaa cgtgatagtt acgcccgcgt
     9481 ccccccgcac ctccatgtca cctttccaca taatatcggt ggatacacca ttcagcagcg
     9541 attcgacatg catggaccca gctcgacagt ggttaccgca tgcgccactg gctcccaggc
     9601 gattggtgat ggcttccatg ccatcaaatt cggtcgcgca cccctgatga ttgccggcgc
     9661 catggaatcg acccagcatc ccctcttcga ggccggcttc gaggcaatgc gagccctctg
     9721 cactgattca aacgatgagc ccgagaaggc aagccgaccg ttcgatgcga cacgtgccgg
     9781 ctttgtcctt ggcgagggcg ccggcatgct ggtgctggag gatctggagc atgcgcaagc
     9841 gcgtggtgcg catatttatt gtgagatcgc gggatacgcg acctccaacg atgcatatca
     9901 tccaatcgcc ccacagccag aaggatttgg tgcggccaag gctatccgag acgcactaaa
     9961 cgatgccggt gttgagcccc atgaggtgga ccatgtcaac gcccacgcag cgtccacacc
    10021 ggccggtgat ctggcggaag ccaaggccat caaactggcg ctcggggatc gtgcagagga
    10081 aatcccggtg acctcggtca agggtgcctt cgggcacagt atggccgccg ccggggcact
    10141 cgaggccgtc atggccgtct tgaccctcca tgaagggaag atcccgccaa cgcggaacta
    10201 caacaaccct gatcccgaaa tcgggctcga tattgtcggc gaggcccggg atgccaaaat
    10261 cagggtaatg acgaagcatt cctttggcct gggtggccag aacgccgtgc tcgtcctcaa
    10321 ggcctgggat gaggccattg cctgaccgtg cgtcgcaatc tcagcctggg cccgccgaaa
    10381 ttggcttctt ctttctagat attgctgctc tggcaggtct tgcccgttgg ggatggcatc
    10441 ttggcggtgg gggattggtg ggcccggctc tcgccctgct tttcgtcctc ctcttcggta
    10501 cggtatgggc cgttttccgg gcccgaggtt tcgtccccaa cggaggagac ccggtcgttg
    10561 ccattcctgg tcctgcacgg ctcggcatcg aactgctctt gtatttgctc gccgcgtgga
    10621 gctgttgggt ttccggttgg caggttgccg ctgtggtgct cgtcatctgt gccattgcca
    10681 tctacatggc catgcgcaaa cgcaccattg gcctcttgcg gaatacacca cctgccgcat
    10741 gagcaccagg tgaggcctgc tgttaccttc gtttccgggt ggatgcgaca gggtccggga
    10801 tcaactgaaa aacgaacgca ttacggtggt ctacgccttg gaggaatgag gtaaatatga
    10861 gcctgaacga aacgctcgga tcaacctacg gtctgatctt ccctggccag ggcagccagg
    10921 cggttgggat gggcaagaat ctcgctgact tctcggacgc agccaggcag gtcatggagc
    10981 gagcggacga gatccttggt ttccccttgt cgacgctctg ctatgagggc cctgccgaag
    11041 ggctcaatga cacctacaac tcccaggccg cgatcttcac cgtcagcatt gccgccctcc
    11101 atgcgcttga ggaggaggca aggcaacacg atgaatactt tgtgcccatg atggtcgccg
    11161 gtcactcact cggccagttc tccgcgatgg tcgccgccga ggtgatcgaa ttcgagaatg
    11221 ccctgagact tgtgcgggag cgtggacgac tgatgaagga agccggtact tcccatcccg
    11281 gcgggatggc agcagtcatt ggaatggacg acctcaaact cgccgaactc gttgatcagg
    11341 ccgcgaacgg tgacgtcctt acgatcgcca acatcaactg cccgggccag acagtgatct
    11401 ccggcgaact agcgccattg gaccggttca tggatctggc aaaggatgcc ggtgcgcgca
    11461 aggttgcccg gttaccgatc agcatcgctt cccactcctc acttatggca gaagcctctg
    11521 cgacgctcaa cgacctcctc gacagcatga cgttccatga gccaaaaatg ccgcttgtgg
    11581 cgaacacaac cgggaaggcg ctcaataccg ctgaagagat ccgtgaggaa cttcgtcatc
    11641 atgccgaacg gggtgtcgac tggactggaa ccatccagac catggttgat aacggcatca
    11701 cgaccttcgg cgagcttggc cccggatctg tgcttgctgg actcaaccgg cgcattgatc
    11761 gcacgacaaa gacagtcggc ttgaaggagc tgggacttcc gtcggcatag gcaagggcgc
    11821 gacatgcggc tagaatggtc gtttagaggg gatgccccga agtatctcgg cgctcgcatg
    11881 ctcaatgttt cgcggacgcg acggcaccac accgggcgct tgtgttgttt ctatgcgtgc
    11941 tacccgtgac cgtgaacaca cggtgtgttg gcagaatcac gacgaatgcc ccgcgagtct
    12001 tccagatgcg acgtagcgct gacgcgggaa acgatcgaag gaaaggacgt catcggtgac
    12061 acgagtggta gtaactggcg taggagcgat tactccgctt ggtatcggcc gtgacgcctt
    12121 ctggtcaggt ctcacagagg ggcgttcagg tatccgatta atccagaact ttgacaactc
    12181 cggcctggag gtgaagatcg cgggagaagt acccgacttc cgggccaagg actttatgga
    12241 cttcaaggcg gcacgacgca tggatcgctt ttcgcagttc ggtgtcgcag ccgcccgcga
    12301 agcgatagag cacgccggca ttgagatcac cgacgacaat cgggaccgca tcggcatcat
    12361 gattaacacc ggtggtggcg gcatcccaac catcgagcat gaagtcacgg tgatggcgaa
    12421 gagtggaggc aagcgggtga gcccgttcct catccccctc tttgccccga acatggccag
    12481 ctgccaggtt gcgatccagt tcaatatcca tggaccgacc gttacctccg tcgccgcctg
    12541 cgcggccggc gtccaagcgt tcgttgatgc ggttcacatg atcgagcgcg gcgatgccga
    12601 tgtagtgatc accggtggaa ccgagggcgg catcaccccg gtggcaatcg ccagcttgac
    12661 aaatatgggc gccctttctc gacgaaacga cgacccgcag agagccagcc gaccgtttga
    12721 taccgcccgc gacggcttcg tcttctcgga aggggccgcg gtagtgatcc tggagtcgga
    12781 ggagcatgcc gcccgtcgtg gcgccaacgt gctatgccag gcaagtggcg gcgcctatac
    12841 gtcggacgcc ttccatatca ctgcgccgga tccgaacggc cgcggcgcca ccacggccat
    12901 gaagcgagcc ctccaacggg cggagctcga tcctaccgat gtcgattatg tggcggcgca
    12961 cgccacggca acccagatcg gcgatattgc ggagacaacc tcgatcaaga acgtctttgg
    13021 ggaccacgcc tacaagctct cgatctcggc gaacaagtca atgctcgggc acttgctcgg
    13081 gggcgctgga gcagtttcgt cactcgcctg tattctcgcg atccgggaca acatcgtgcc
    13141 gcctacgatc aatctggata accaggatcc ccagtgtgac ctcgactaca cacccaacgt
    13201 cgcaaggaaa gctgaaatca agaccgccct ggcaaacggg ttcgggttcg gcgggcagaa
    13261 tgcggtgacc gtcttccggc aaatctagcg ccagccacaa tctcatccgt gatcgacccg
    13321 gaatcttcca tgcaggattc tctatcgccc gtttaccctc atgtacccga tcggactccg
    13381 taaagaccgc gcctgttccg ctctgcacgc caattgaagg gatcctacct catggctcat
    13441 gatgtagcgc tcgtcaccgg gagcgcaaag ggaatcggtt atgcgattgc acgtcgactc
    13501 cttcgcgatg gcatgacggt gattgtcgcg gacatcgaca gccgcggagc cgagtcctcg
    13561 gcggcagaac tcggtgatcg ggcacatgcg ctgcacctgg atgtcacctc ccacgagaga
    13621 tgggaatcag gcgtaacatc tgtagccagg cagcatggta cagtctccgt cctcgtcaac
    13681 tgtgccggga ttgctggccg ctccgcgcca tgttgggaat tgagtccgga agagtggcat
    13741 caagtcatcg ggatcgacct gaccggtgtc tttttcgggt gccgtgccgt gctccccgca
    13801 atgatcgacg ccacatacgg acgcatcgtc aacatcgcgt cgattgccgg caaggagggc
    13861 aatcccaatg caagccccta ctctgcagcg aaatccggcg tccttgggct gacaaagtca
    13921 atcgccaagg aagtagcggc caagggcatc ctcgtcaacg ccgtgacacc tgccgtaatc
    13981 gagacggaaa tcctcaaaca ggtcacgccg gaacatatcg cctacatgac gagccggatt
    14041 ccgatgggcc gcgttgggca accggaggag gtagcagccc tcgtcagctt cctttgctcg
    14101 cgcgagcttt ccttctccac cggtgccgtc ttcgacatct ctggtggccg agccacgtat
    14161 tagtgccacg gagattcctg aatgactccc cgattaaaaa acggacacgc aacgggaacg
    14221 gtccacctcc tcatacacga atcggccact gcttccatgg ccgttacgcg ctcgctcacc
    14281 agttgacggt acaataggcg ccagtatcgc ctgatccatg aacgggcatc aggcactcga
    14341 cccacacccc gctggaggat caccaggaat gagctttcca tggagtcgca agtcccgacc
    14401 aagcaaggaa tccatgatgg tcgaaccgcg accggaaatc tcgggccacc aattcaccct
    14461 caaaggctcc ccccgccact acgatgacgt ggagaagatc atcgagcaag gcggtggtgc
    14521 aacggtctat ttcggtgagg cactcacata cgagcgaggg gccggggtcg cccagtggcg
    14581 cgtgaaggcg aagagcctgg attggcttcc ctcgctgtat gactggtggg ctgaggcgga
    14641 acgcgttgaa ccgatcaact tcacgttcca tctctaccac cctgagaacc tgggataccc
    14701 tgttttggac ctcagtcagc acacggccga agaagttgca gaatacatcg ccgacaacat
    14761 gccctgggtc ggcagtgatc ctgtcacgac caagccgtct cggatgtcct ccggcgagcc
    14821 cacgatgaac gcccactaac cgccagcaag aacagtacgc aatacgggat ggcaggaatt
    14881 caatctgcca tcccgttacc cttgccctgc ctagttacca ggtgtgggca acccctggac
    14941 tgacgagccc acaactgccc ttcaaatcca gcaggacacc cggggcgcaa tccaatcaat
    15001 cggcggcggt ctcttctccc ttgagctccg cctcgatcag cggccgcagc gtctcaatca
    15061 aatcctcgag atgctcaggg tgttccttcc agcactgatt acgggtaggc gtgtgcatac
    15121 acaacaggac ctcgtcctcg tcgtgcactg cccatcccac cgaacagcgc ggcacatgtt
    15181 ggccacctcg aaacggcatg cccagcctgg ttcgcgtcaa catcgggcac ccaaaggtgg
    15241 gctccgttgt atcttcggcc tcactcaggg gccgcttccg catcggctgg atgggagcat
    15301 cggcctccgc atccctccgg atcacttctc ccgggattgg ctcagtcggc ttccgccggc
    15361 ctccccgtcg acggttggta cgaccggccc cgtcacgctg ttgcggctga ggcgcacggt
    15421 tttcggctac cggcagtggc tggatcggct cctgcggctg cgcttcgcgg cgtggagcac
    15481 ccccgccaga ttgtcgcttc cgactccggc gacgcggccg gctagagcgg tttttctgac
    15541 cgcccggcgt ttctgcgttt gagttgctca cgagcgaacc cgaacgcggc tctgctgact
    15601 cgttcaagag cctttcctcc gactttgatt gatttcttaa cggccatgcg aacaacatcc
    15661 cgcacggctt gaagatcggc gtcgatttcc tcgcggctca cgtgcgccaa tgcgagattg
    15721 cggttagcaa tctccgtcgg catcatctgc aacgatttct ggatcgccgt gagcagcaca
    15781 tacatgtcgt ctatttgacc ggcgaccggg atcacgcccg gcaccagatc gatcggcgaa
    15841 accgcatagc caccaccgac tgcgaggacg gtcttggcct gtttcggcac atcgggatcc
    15901 ctggcgatgc gtgccaccag cttgatgtac gcaggtagtc tcttgaccgc ctcccagaaa
    15961 cgctgcatcg catcggacga ggttccgtca attggctcgt ccggctgctc ctgaggcagg
    16021 atccctggcg tgcgttcgtt tgttgatcgc ccggggatat tcacatcggg agtgccctgc
    16081 acgggttgtc taccctgcga cgcttcatcc atcaatggcc ctccttccgc tgaagctcaa
    16141 aacattccgg tcatataggg aggtattcgc tccccatacc tctattgtgc cacccgcatg
    16201 ccaatgccct gaggcgacat agctgccatt ggaccgcgat gagacacaac cggaacgccg
    16261 cttcaggcac gtccggaacc ccgtcagctg tgaaaaagac cagacaggac gtgggtggga
    16321 gaagcacgaa tccgggcaac taccgatcga acacctgtcg caacacgaag aatgcgttcg
    16381 atggccgctc cgcgatccga cgcgtgaagt agggatacca ctcggttccg aatggcacat
    16441 agacgcgcat gccatacccg gcccgcttca gacgcaactg ctcgttccgc cggacaccat
    16501 aaagcatctg gaactcgtac ctgtccggcg ggatcgtgtt tgcccgtgca tacgcgatgg
    16561 ttgcctcgat caacgccggg tcgtgcgtgg cgattgccgg gtagttcccc ttatccagca
    16621 aatgctgcat caaccgctcg tagttctcgt cattctcggt tctggactgg atagctaccg
    16681 attctggctc tgcataggca ccttttacga gtcgcacccg tgcgccacga ttgatcatat
    16741 ctacgacatc agaggcggca cgatggaggt agctctggat aacgatgcca acctcttccg
    16801 gaaaccggtc atggagctgt ccaaagatct gcatggtgcg tgtcgtgtag tccgacccct
    16861 ccatgtcgat gcgaacgaac ccgcccacct cccgcgcggc ttcgaggatc cgggtcatgt
    16921 tctcccgggc aacatcgtct cccaggtcaa gcccaagcat cgtgagcttg accgagatgt
    16981 tcggctcgag atcgttcgcc ttcatctgac gcagcgtttc ggtgtagctt ccgacagcgg
    17041 ctgcggctgc atccctggtg gagacattct caccaagcag gtccagcgtg acggtaatgc
    17101 cctcccctgc gagctcccgg gcgactgtca gcgcagattc cagcgtatct ccggcaacaa
    17161 accggttcac caactggcgg gcataccggt ttcgcacaaa cagccggtgt accggaggca
    17221 ggtctgccac ccccagcacc gctcgccgca tcaggttcat tgtccttact ccatggctgc
    17281 tattcacccg ccgtcaacag gctacatcgc ccgggcgggt atccctgtgg agggatgtct
    17341 ttgctctgaa ttatcccatg cgggaagagt tccgcgatca ggaagcagcg agccagtcca
    17401 gcaccacagc agccgtccat gactggtcgt cggatcccag tgcctcaccc gtaaaaggct
    17461 ccacgtactc ggcaaatcca gcagaggaaa tctggtcgag tgcgttgcca cgcagggccg
    17521 ccgcgatctc accctcgccg atgcgttccc atgcccacca gagcaaccag ttcatcaccg
    17581 gccacaccgg acctcgccag taccggtttg gaacaaaaga cggctcgcca gggctcgtgc
    17641 ttggcggcaa tgcccagcgc aactcagggt ggccggtgaa ggcttcggag ctccaaaccc
    17701 ggttgagggc cgtgagtgta tcggcggtta attggccggc gaggagcggt gcaaacgtag
    17761 caatagtctg caccttgatc catgagttat cgatcacatc aaggtcacag cacagaccct
    17821 cgtcggggag ccactgttcg gcgagaccac gacggccccg atcaatccac gactcgatga
    17881 tcgtcaaatc gtcgaagtca ccatccgcca gggcggcgat acggatcagg gcttcgttgg
    17941 cggcgaccag catggcgctc atgaagatgt ccttcacccg gaagggcaac tcatcataga
    18001 tttggtccac gcggtagctg cctcgcttca gcacctccac gatccatatg aaccgttcat
    18061 agtcctcgtt ggtcgggcgc tggctcgcat cggagacatg cgagagatct ctgcgttcga
    18121 atggaggcac tccaccctcg ggaacctgaa tgcgtgccat tgcctgatcc caacggggtg
    18181 agttgtccat cccgctctcc cagggatgca cgatcgtaat caaacccgac acctcagggt
    18241 cccgcgccga aaccaggtac cggtgccatt ccaggagcgg accgtagaac tccgatgcga
    18301 tggctcgcgc ctgctcggga ttcgcctggt cacagatgcg acgtatcgcg attgcgtgta
    18361 tcggtggttg aatgatgccg ctggtcgcaa tgccttcagg cacatccggt gagacatgtg
    18421 cgacatccca gcgatccgcg tcggggaaat acgcgtcctt ggggacgtcg gcactgtaga
    18481 cgatgtgagg gagcattccg tttttccact ggcctcggaa cagggtgcgc atctcctgga
    18541 tggcacggtc cagggaaagg tgcaccaggc cgatcgagac aatggccgca tcccagctcc
    18601 actgatgcgg atagagcgca ggcgccgcct tgatcgacgt accagtatca ttggcccgaa
    18661 ggatagccgc tgcgcgtgca gccatggggt tgtcctggtc cacactcatg gaagccgcat
    18721 ctcctcgtta ccgtggaaac agattcgaac gttccgattt gacggattcc gtatggaaga
    18781 ggatacagtg aaaccccgct atctcataca ggaatggcca gacctgaacg aggcaacgtt
    18841 tcacccttga tcgaggatat tttggaatac cgcgacgtta tcggcacctc accagctccc
    18901 caggagcgct ataccagtgt catcagggat gggctgtatg accgcattcc gatgatcgcc
    18961 cccgccattg cgctcatcag cacgttgccc ttcctccgac tggatcgcat ccagcaactt
    19021 ggctttgtct cgcgcgtttg gcctggcgcc aagcacaccc ggtatgagca ctcacttggc
    19081 gtgatgcacc ttacccggct ggcggtagag cacctgtgca gtacgcccga aggcacctgg
    19141 atcacaccgg aggacgcccg gaccgtcgtg gcggctgccc tcctgcacga catcggacat
    19201 tacccgttct cccacgcgat cgaggagatc ggggcgcccg tggcgacaca tgaggcggtc
    19261 ggtcaggtaa ttatcgagag cggcgagatt gcggagattc tcagccagga ctggctcgtc
    19321 gacccggctc gtgttgccgg cattatccac gacgatcggt cattgcttaa tgaggcggat
    19381 ctcgtactcg gtgggttgct ctccggaaca ctcgatatgg acaaactcga ttatctgcca
    19441 agagacgccc gcgcgtgcaa cgttccctac ggtggagtag acacggcccg gctgatcgaa
    19501 tccctgacga tcgttgaggt cgatcttcct ggcaccagaa cctcacagcg aatcgcgatc
    19561 actgaaaagg gcatcagccc gttgcactca ctcatcaatg ctcgccagga gatgttcgac
    19621 aacgtctact ggcatcacac gaatcgcgcc tgtatggtca tgctcttgcg ggctatccag
    19681 gatgccctgc ttgctgatgc gatctcacca ggtagtctca ctggccacga cgacgcatcg
    19741 ctcctggcat tcatgcgcag tgtcgacatg cctgccgcaa cccggcggct cgccgatgca
    19801 ctcgccgggc gccggattca caagcgcgcc gtggagatca gcgcccgtgc cgagtcactc
    19861 tacagccgcc tcgcacaact ctggtacgat cagcgcgccc ggcgtgaggt cgagcttcgc
    19921 cttgtcgcgg ccctctccaa cacacttggg gaacccgtcg aggataccgc gatcctcatc
    19981 gatatcccga agccggagaa gtggaagaca gacgtctggg tgagtttcag gcaaccgccc
    20041 gttggcatgc agccgctcat gcactggcag gacgtcgtgg gcctcgggga cgaggacttc
    20101 aagcgctatg aagagcatcg acgcatgatc cggatcgtca cagaagaacc ctaccggcac
    20161 gttctggccg aaacgtggga ggccctcctt taccccgcac tcggtggggc actgtaaccc
    20221 aaagaggtac catgaccgac caacaacaac tcgcagatct tcaaaccccg gaaccgtcac
    20281 aggcagaaac catcatccga ccggctcgcc cggatgacac cgtgcaatcg gatagggagc
    20341 gggcagacac cgaccttcct gaaaacgacc cacatggcac cgtctctatc gttctgatgg
    20401 gcgtcctgat gctgatgctc gtcctcctcg tcctggtcat cgtacaggca gcgtaaggtg
    20461 agcagaccta atcgcctgcc atccgttcct acgattcctt gcgccggtcc tcctcagcga
    20521 tgcgttcacg cgtgtggccc tcgacctctg taccggccgc cactggctca ttcacgctgc
    20581 ccgaggtcac accggccagg ggtgcttcct ccatcagggt gtggtgtatc aactttgcgg
    20641 atgccgcttc cgtgaggccc ggcgccagat gacttcccag cgccgtgccc tttgacttga
    20701 ccccgacatc gacctcgtcc ttgggatcat cgatcaagcc aacgatggca tccacgacaa
    20761 tctccgggtc atcgatcatc ggcatccgca ggctgtgccg cgtgtaattg cttccatgct
    20821 cgaagaacga cgtatcggtc gcccatgggt tcacgacgca tacgtgaata ttatcttgcc
    20881 cattcagctc aagctcccgc cggatcgagg cactcattga gagaatggcg ctcttcgttc
    20941 caccgtagac ggcgaagtac ggcatcgcga ccttccccgc aacggacgcc acattgatca
    21001 gcgtgccttc accatgctcg acgaactgct gcatcgcgac ataggtaccg ttgagcgcac
    21061 cgatcaggtt cgtctccacg acgctaatct ggtccctgag cggaaggtcg gtaaaccggc
    21121 cgatcgtccc aaccccggcg tcattgatcc agacatcgat tcggccatat tccgctttcg
    21181 cccgctcgcc cagatagtgc acctcgtccg ggttcgcgac atcggtcggg acggcaatcg
    21241 cgtgaccgat ttcacgcgcc agttcttcga gaagctcacc tcttcgcgcc gcaagcacga
    21301 gattatgccc ctgctccgcg agcctctgcg ccactccctt gccaaatccg ctggatgctc
    21361 ctgtaacaac gatcgtcttt gccatcatgt tctcctctta cgcgcagccg gaaagttagg
    21421 gaagatacgc aaacagcggg cccacagggg cccgctgttg agcacgatct gcattgttgt
    21481 agggattatt gcagcatcgt tactgcgccc tcggaggcct gctggacatg cgcagcgtac
    21541 ttggcgagca cgccctgctt gtaacgcggc tccggctggg cccagtcacg cagccgagtg
    21601 gcgatctcat cctcgctcag gtgcacgttc agcgctcgat tctcgatgtc gatctcgatc
    21661 aggtcgcctt cctgcagggc ggcaatcggg ccaccgacaa acgcctccgg cgccacatga
    21721 ccgatcatga ggccgcgtgt ggccccgctg aaccggccgt ccgtgatgag cgcgaccgag
    21781 ccgccaagcc cctgccccac gatggccgcg gtgatcccga gcatctcctg catccctggg
    21841 ccgcccttcg gtccctcgta gcggatcacg acgacgtccc ccggaacaat ccggttgtga
    21901 agcaccgcgt cgcccgcctc atcggccgaa ttgaagacgc gcgctggccc ggtcagatgc
    21961 atcggctcat acccgaagag cttcaccacg cagccgtccg gtgcgaggtt ccccttcagg
    22021 atgttgagac caccggtagc cttcatggga ttgtcgaacg catggacgac atcctggcct
    22081 gcggtctccg tggccgtcgc tgcctcgtct gcaagtgatc tgccggtcgg cgtggcctgt
    22141 gacccatcga tgtacccggc gtcgacaaga cgctgcgcca ccagttgggt accgccagcg
    22201 gcatcgaggt cggcggcggc atatttgccg cttggcatca ggtcggtgat caatggcgtg
    22261 cgacgggaga tgacgtcgaa atcctcgatg gtcaacggca ctccggcttc ccgcgccagc
    22321 gcgaggagat gcaggaccga attggtcgat ccgccgcttg ccgcgacgcc ggcgatcgca
    22381 ttctcgaacg ccccccgtgt caggatgtcg agcgggcgct gattgcggcg aagcatgtct
    22441 atgatcagct cgccagcctt gagaccgacc tcttccttac gcggatcgat agcgggcggc
    22501 gaggcgctgc caagcggcga aaggccgatg aactccatag ccatcgccat ggtgttggcg
    22561 gtgaactggc cgccgcaggc gccagcgcca ggacacgcgt ggttctcgag atcctttaga
    22621 tcctcatcgc tcatcttgcc ggcagcgttc gcaccgatcg cctcgtagac ggtctggatg
    22681 gtaacttcct tgccctgcca gtagcccggc atgatcgttc ctccgtagag gatgaaaccg
    22741 ggaacgttga gacgcgcaag cgctatcgag aggccaggca acgtcttgtc gcaggcgccg
    22801 agagcgatga tgccatcaaa catatgcccc cggccgacaa gttccaccga gtccgcaatc
    22861 acctcacggg agataagtga ggttttcatc ccctcagtgc ccatggtcac gccatcggag
    22921 attgcgacag tgttgaactc catcggcgtg ccgcccgccg cccgcacgcc cgctttcacc
    22981 tgctcggcga ggcgccgcaa gccgaagttg cacggcatcg tttcgatcca ggtattcgcg
    23041 ataccgatga tcggcttctg caggtcttca tccgtgaagc cgatggcctt gaccatcgag
    23101 cgggcacccg cgcgatcgtt accgtcgtac agcgtcctgc tgttgaagcg catgtcgacc
    23161 tgttcgcttt ggccgttgct gccgtttgcc ttgccatccg tgctcatgag tacagtctct
    23221 ccatcgtact tcgtcgttat gccgcgccgc cgggaccgtt cacctcccgg aagcgcgact
    23281 acacaggtta tccatcacca cagtgcaggc ttcattgtcg gcagttggtc gacatcgttg
    23341 tcggaaattt cccatccagt gtcgggtctg gcgcgaatat tggcaagttg cgaaactcgt
    23401 caatacgggt aacaatgccg ccatgcaaca tccgaaggaa tgctctcagg agacctacct
    23461 caatgactga ccggggcgtc cgtgaatttg gcatgcagac gattcagtac tggacatggg
    23521 aggagactcg cgagcgctgg atatggttcg aggatctggg attcgagagc ctctggctca
    23581 ccgatcactt tgtccggact gcgcaaccgg agagccccta ttttgaagct tggacacttc
    23641 tcagtgcgct cgccggtgta accaggaacg cgcgcctcgg cgtgctggtc agcagcaaca
    23701 ccttccgtca tccttcgctc ctcgcgaagc aggccgtcac cctcgaccac atctctggcg
    23761 gtcgtctgga gctcgggatc ggagcgggat ggttccagcc cgagcatgag atgttcgggc
    23821 tggagtttcc tcccacgggc gagctcgtgg atcgctatga agaagcgatc gatctcctgg
    23881 accgctatct tcgcaacgac atcacgagct tcgaaggtgc ctactattcg ctcaagcacg
    23941 ccccgaaccg gccaacaccc gtgcagcagc cacggatccc gttcgtcctc ggtgcccaca
    24001 agccgcgaat gatccggctc gccgcacgct acgcggatac ctggaactca cggggcaccc
    24061 cgggggagat tcgcgaccga aacgacctgc tggacgagtc ctgcgtcgag gaagggcgcg
    24121 acccaaagga gatcaagcgc tccctgcttt acgtcatcgc ccagatgacc gacgagcgcc
    24181 cctgggactc catcgatgcc tttgaggact tcgtgggccg attcacggag gcaggcatgc
    24241 aggagttcat cttccagccg ccggacccgg agcattatgc gatcgtcgag cgcatcgcca
    24301 ccgatgtgat gccccggcta cgcgggtcag acacggcata aatttggcga tacatctgtt
    24361 ggctgggccg aggtgtgggg tgtgcatgta tccagcgccc atcagggaag cgtcaccggc
    24421 agcacgacca cgcactatac tttgggcaca atggccatca tcgccacatc gctcccattc
    24481 atgaaagcag gaatatcgtt gggcatcaca gagctgctgc taaacattct cagtatctat
    24541 tcattcgtcc tctttggccg tgccatcatg tcttggtttg accccggttt caggtccacc
    24601 atcggaagaa ttctctacga catcacggag cctgttgtgg ccccggtccg tcaggtcgtc
    24661 ccatcgctgg ggggcctaga tctctcgatc atggtcaccc tgtttctgat cctgatcctg
    24721 cagcgcctgc tttcatcggc actgtaagaa agcgcaggcg ctgccgttgc tcgggtcatc
    24781 cggctggacc ggccctgaga aatctcttac attgtgttcc gtaccggttg tccgcatgcg
    24841 cacgcacgga gaacctctgt cacaacacga tgatcgcgcc ggctgggttg cccgccagct
    24901 cgatccgacc aggagccaca tatgttgctg aggctgctct ttctgatttc attactggga
    24961 tatgcaaccg gccccgatgc ccgcgaacgg gaagaggacg acaagctcgg tggtgaaggc
    25021 ttcggtacga tcgtcatgct cttcgtcggt ctcggcttgt tgctcctgtt ggcactggtt
    25081 gttctggcaa ttctcgcggt aactggccag ttcgattccg agttcatgga tccgatcacc
    25141 tgatccaaaa tccagcgaat aaagcggccg ggagcctcgc ccccggccgt gcctgtttcc
    25201 tcacgtttta agatcaaacc caagcatctc catcagtgcc aacgcattgc tggatgttgc
    25261 caaccccggt cgcagccggt agtcgaaggt catcgtcggc aaatccggac caggcaagaa
    25321 aacctccgcg aaatgcacca gcgttgccga atcttttagg gtgctcccct cgatcagctc
    25381 cagatcatgg cttgagatcg ccccgaatgc ccgttgttcc accagcgaag ccaggacacg
    25441 acgaggggcg atctgacgct cggcggtgtt cgtgccctga aggatctcgt ccagcaagta
    25501 cagggcagtg cgggagggat cggacgcgac tccatccacc acagccttca gccgctgcaa
    25561 ctccgccata aagaacgaaa caccttgctc cagcgaatct tccacccgca tgcatgtcca
    25621 gagccggaca ggtggcatgg acagcgaccg ggcgaagacc ggtgccccgg cctgcgcgag
    25681 aacaacgttc gccccgatag cccggagcag tgtgctcttg cccgacatat tagagccggt
    25741 gacgaagagg aagtgtgctt ctggccctac cgtgacgtca ttggcgactg cttcctgctc
    25801 ccggagcagc ggatgcgaca gctctgccgc gaaaagcgaa actgcctcgg gcgtcacccg
    25861 cgggaatgcc cagccgggat gatcgcggtg caggatggag aatgaagcca atgcttccca
    25921 ctctccggcg gcccgcaacc aggcatgcac tcggtcacct gccttggctt gccatccctc
    25981 cagcaactcg agcacgtgaa tatcccaaag gaaggcaagc tgaagcggga aataaataat
    26041 cgcccctggt gggatcgcca gggagacagc tcgagcaagt tgggccgtcc gtgccagcgc
    26101 accatcatgc ccgccttcaa gcgtgtcatc gatatgccga agcagggacg cccgcgacct
    26161 ggccgtcgca atggtccaga agatgtcgcg gtacccggcg attgcatccc gtaagggaac
    26221 gatcgcttgc acccgcaccg ccgcctgctg gccccatagc gcaaagatga gcccgttcag
    26281 cgccacgggg atcacaaaaa gcggcgagct ggtaacgccg agagcccata acgccagcaa
    26341 gatcacgaac acaaccgggc tgacccaact gaccgcgcgc aaacagatcc tgggacgaag
    26401 ccatggcgtg gaccccgccc agtgaacgag cgcctggggg tctggcaacg gctcatcgtc
    26461 gcgaagcgca gccgcctcca ccgcctgctg cacatccacc tcgccggcca gctcggcaat
    26521 cgcctcctgc cgcacagcaa ggtcgtcaag ggacgaggta gttaacaccg agcggtagag
    26581 cgcatcccac ccgtagcgag tcgctggtat ggcgattcgc
//
LOCUS       DASYWM010000040         4230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_264234, whole genome shotgun sequence.
ACCESSION   DASYWM010000040 DASYWM010000000
VERSION     DASYWM010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264234"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="VGR29_01610"
     CDS             complement(<1..1716)
                     /locus_tag="VGR29_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072313.1"
                     /translation="MSRTSFIGREREIRLVQSLLNRDDISLVTLTGPGGVGKTRIAVR
                     VAQSIPHAVHFIDLVDVHEPSLVLPTIASAFDCAPNGRSVIDALSAVLRDGDHVLVLD
                     NFEQILSAASSLVHLLDALPRVKVLVTSRAVLGLPGEYVVDVSPFALPGPDGQLSVDA
                     LAGHDVVRLFVDRVRTLQPDFALSSANAATIAKICQRLDGLPLAIELAAAWAPVLSPS
                     ELLAQMERPLALPDAGSHSAHIRQRTIRDTIAWSYSLLDTSSQTLFQRMAIYIGGCTL
                     DSINEVCGDENLKPLQGLHRLVAHSLVRRMDLPSGESRYTMLETIREFGMERLDQSGE
                     GDILRQKYAQYFLAFAEHAEVMLNTDEHEIWLDRLEADQGNLQNVFDWALKHKDAEFA
                     LRLSGALWQFWRFRFFSSSGQEWIQRALALGQDVSAPVLRKALFGAGSLAWAHGRYAE
                     AGTMLTDSLAMYQETDDPAAIGHVELALGRLAWDQGKQDGARRRFEEASRLFEQADDR
                     SGLAHGLHGMGLVAYKDGDYQLSASYFQDALASWQSLGFSWGLACCIPGHLGEVARAE
                     GHLSRA"
     gene            complement(2236..3150)
                     /locus_tag="VGR29_01615"
     CDS             complement(2236..3150)
                     /locus_tag="VGR29_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2072314.1"
                     /translation="MATPSLSYEEQAQSVPHVGVMTPKKRKPEPRVNGKDIVLGILMV
                     LLALAFIFPFVWLIMTSFKPPAEVFSGEFFPSRWTTQNYEQVFTQVPMWRWMGNTFLV
                     ALLGVVSVVLSSSLVAFGFARLHFKGRNALFALVIGTYLLPGSITMIPTFLIWNELGL
                     VGTLYPLWAGNLFGSAFYIFMLRQFMLTIPQDLVDAARLDGASYLRIYWAIMLPLVRP
                     AIVAVAIFEFNAKWNDFMGPLIYLNNPDRYTLALGLQSLKSNFAELGTQWSLLLTASV
                     IFTIPMVIVFFLFQRYFMEGLAHTGIKG"
     gene            complement(3166..>4230)
                     /locus_tag="VGR29_01620"
     CDS             complement(3166..>4230)
                     /locus_tag="VGR29_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2072315.1"
                     /translation="GLESMLSRPSRAANRFTYPIAGRVTASAVGTRCDFLNGFTDMAV
                     VSRPNAPSTTKRRFSDETISGWLSISPWLIGFVVFTATPFLASLYFSFTDYNVLNPPE
                     WVGLKNYERLLTDDRLLPKALQNTFIYAMMYVPLNIISALAVAMLLNKARHFTGFFRT
                     AYYLPSITPGVATAYLWVWILNPNDGIVNSTLRSIGLPAPGWTVDPFWTKPTIVITEI
                     WTLGASMLLYLAALQGVPKDLYEASTLDGAGAWRKFKDVTLPMISGITFFVTTISVIS
                     ALNVFTQGYVMFDEDGGPDNSALFVVMYLFKRAFGSGYFQMGYASAIAWVLFLIILVV
                     TFIQFKFSKRWVYYEHDTRN"
BASE COUNT          942 a         1192 c         1281 g          815 t
ORIGIN      
        1 cgccctggaa aggtgcccct ccgcccgtgc cacctcacca aggtgaccgg gaatgcaaca
       61 cgcaagcccc caggaaaaac cgagtgattg ccaggatgcc aacgcatcct gaaaatagct
      121 ggcgctcagc tggtagtccc cgtccttgta ggcgaccaac cccatcccgt gcagcccgtg
      181 cgccaggccg ctcctgtcgt cggcctgctc gaagagtcgg ctggcctctt cgaaccgacg
      241 tctggcccca tcctgtttcc cctggtccca cgctagtcgc ccgagagcca gctcaacatg
      301 cccgatggca gccgggtcat ctgtctcctg atacattgcc agcgagtccg taagcatcgt
      361 ccctgcttcg gcgtaccggc catgggccca tgcgagcgat cctgcgccga acaatgcctt
      421 gcgcaggaca ggggccgata catcctggcc gagcgccagg gcgcgttgta tccactcctg
      481 acccgagctg gagaaaaagc gaaatcgcca aaactgccag agcgctccgc taagtcgcag
      541 tgcaaattcg gcatccttgt gcttaagcgc ccaatcgaaa acgttctgca ggtttccttg
      601 gtcagcttcg agccgatcca gccagatttc gtgctcgtca gtgttcagca tcacttcggc
      661 atgttcggca aatgcgagga agtattgggc gtatttctgc cttagaatgt ccccttcacc
      721 actttggtcg aggcgctcca tgccgaattc acggatggtt tcaagcatcg tgtaccgcga
      781 ttcgccactt ggcaaatcca tacgccggac caggctgtga gcgaccaatc gatgaagccc
      841 ctgcaggggc ttcagattct catctccgca gacttcattg atggagtcca gggtgcagcc
      901 accgatgtag attgccatcc gctgaaataa cgtctgtgat gaagtgtcga gcaggctgta
      961 actccatgcg atcgtgtccc gtatggttcg ttgtcggatg tgcgcgctgt gtgagccagc
     1021 atcaggaagg gccaaaggac gttccatctg ggccagcaac tcggatggcg aaaggactgg
     1081 tgcccacgcg gctgccaatt cgattgccaa cggcaacccg tccagacgtt ggcagatctt
     1141 ggcgattgtc gcggcattcg cgctcgagag ggcaaagtcg ggctgcaggg tccgcacacg
     1201 gtcgacaaag agccgaacga catcgtggcc ggcgagtgca tcaacagaca attgaccgtc
     1261 tggtcccggt agggcaaatg gtgaaacatc aacgacatac tcgccgggga gtcctaacac
     1321 cgctctgctg gtaaccagca ccttcactct gggtaaggcg tctaaaaggt ggaccagcga
     1381 cgatgcggct gaaagaattt gctcaaagtt gtcgagcacg agcacatgat ccccatcgcg
     1441 tagcacagcc gacagcgcgt cgatgactga gcgcccattc ggcgcacagt caaatgcaga
     1501 ggcaatcgtg gggagcacga gcgagggctc atggacatcg accaaatcaa tgaaatggac
     1561 agcgtgagga attgactgag caacgcgaac ggcgatgcgt gtcttgccga caccaccggg
     1621 gccagtcagg gtgacgagcg agatatcatc gcgattcagg agcgactgaa cgagcctgat
     1681 ttcccgttcc cgtccgatga acgatgttcg ggacaatggc agtccctccg gtccagggcg
     1741 cctgtggacc ggtgttgaga agatacaggt gggtccctcc gagcttggct cgggaaccac
     1801 gaaatctgcc ataggctcgc gcccctcgcg acatgaagga tgatcctcca tcatcatgag
     1861 gtgcagcgaa ccatatgtca attcatggcc actcactcgc gtcgttttag caaggttcgt
     1921 gaattctatg gcgctttcgg cggcaggatc ccagttcggc ctacgaatct acgcaagcgg
     1981 tgagggacga cgtttccttg cctccagtcc cttgctgtct atgagcgctt tggtgatgat
     2041 ctctacgctc cctcaaaggg gttgtgttct gcttgcagga gtgtcccgac aggatcgtaa
     2101 cgttcctccc cgcgttgatg tacgcgagag ggccgcccta tttgcggccc tcttgagtgg
     2161 aaacatcacg gtcgataggt gtggttccgg aagaggctgg atctcgtcca cctgcaggtt
     2221 acacagccct cgcggttagc ccttgatacc ggtgtgggcg aggccttcca tgaagtagcg
     2281 ctggaagagg aagaagacaa tcaccatcgg gatcgtgaag atgaccgagg cggtcaggag
     2341 cagcgaccat tgggtaccca gctccgcgaa attgctcttc aggctctgga gaccgagcgc
     2401 cagcgtgtag cggtccgggt tgttcaggta gatcaacggc cccatgaaat cgttccactt
     2461 ggcattgaat tcgaagatcg ccacggcgac gattgccggc cggaccagcg gcagcatgat
     2521 cgcccagtaa atgcggaggt agctggcgcc atcaagtcgg gctgcatcca ccagatcctg
     2581 tgggatcgtg agcatgaact gccgcagcat aaatatgtag aaggcggacc cgaaaagatt
     2641 cccggcccac aacgggtaga gggtgccgac cagcccaagc tcgttccaga taaggaaggt
     2701 cgggatcatc gtgatcgaac cgggaagcaa gtaggtaccg atcaccaggg caaagagcgc
     2761 gttcctgccc ttgaagtgca ggcgggcgaa gccgaaagcc accagcgagc tggagagcac
     2821 caccgagaca acgccaagga gggcgacgag aaaagtgttg cccatccagc gccacatcgg
     2881 cacctgcgtg aagacctgct cgtagttctg ggtcgtccag cgggatggga agaactcgcc
     2941 ggagaagacc tcggccggcg gcttgaacga ggtcatgatg agccagacga agggaaagat
     3001 gaacgcaagg gcaagcagga ccatgaggat cccgagcacg atgtccttcc cgtttacccg
     3061 tggctccggc ttacgctttt tcggggtcat gaccccgacg tgcgggaccg actgggcctg
     3121 ttcctcatag gaaagcgatg gcgtcgccat gatcaggttt cctccctagt tccgcgtatc
     3181 gtgctcgtag tacacccagc gcttggagaa tttgaactgg atgaaggtta cgaccaggat
     3241 aatcagaaac agcacccagg cgatggctga ggcgtagccc atttggaagt agccgctgcc
     3301 gaaggcgcgt ttgaagagat acatcacgac gaagagcgcg gaattgtcag ggccaccgtc
     3361 ttcgtcgaac atcacgtagc cctgggtgaa gacgttcaag gccgagatga cgctaatcgt
     3421 ggtgacaaag aaggtgatgc ccgagatcat cggcaaggtg acgtccttga attttctcca
     3481 ggcgccggcg ccgtccagcg tggaagcctc gtagaggtct ttcggcacgc cctggagtgc
     3541 ggcgagatag aggagcatgg aggcgccgag tgtccatatc tccgtgatca cgatcgttgg
     3601 cttggtccag aatggatcga ctgtccagcc cggggcgggg agaccgatcg agcgcagggt
     3661 gctgtttacg atgccatcgt tcggattcag gatccagacc cacagatacg cggtcgcgac
     3721 gccaggtgtg attgacggca ggtagtatgc cgttcggaag aacccggtga agtgccgcgc
     3781 cttgttcagc agcattgcta ccgcaagcgc ggagatgata ttgagcggca cgtacatcat
     3841 cgcgtagatg aacgtgttct ggagcgcttt cggcaacagg cgatcatccg tcaagaggcg
     3901 ttcgtagttc ttaagcccca cccattcagg agggttgagg acgttgtagt ccgtgaaact
     3961 gaagtagagg gacgcaagga acggtgtcgc ggtgaagacg acgaacccga tcagccacgg
     4021 agaaatcgag agccatccgg agatagtttc gtctgaaaac cgccgcttgg ttgtcgaagg
     4081 cgcattgggc ctggaaacta cagccatatc cgtgaatccg ttcaagaaat cgcaccgggt
     4141 accgactgcg gaggctgtta ccctaccggc gatcgggtag gtgaatcgat tcgccgcgcg
     4201 ggatggcctg gaaagcatgc tctccaggcc
//
LOCUS       DASYWM010000041        15487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_207847, whole genome shotgun sequence.
ACCESSION   DASYWM010000041 DASYWM010000000
VERSION     DASYWM010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15487
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207847"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VGR29_01625"
     CDS             complement(<1..288)
                     /locus_tag="VGR29_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2072316.1"
                     /translation="MESRQESEPLLQMQDISKAFPGVIALSNASLTVRRGEVHALVGQ
                     NGAGKSTMMKILAGAYRRDSGTIVFNGQPVDFRTPQQAQASGVSTIYQEINL"
     gene            complement(469..1521)
                     /locus_tag="VGR29_01630"
     CDS             complement(469..1521)
                     /locus_tag="VGR29_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072317.1"
                     /translation="MDRVITRRRLVQVSAAGAGAVMLTPMLLRTALAAPPPLEKKAKY
                     KVGFSQVEMNNPWRTAQTASMQAEAEELGHELVYTDAGGSEAKQIADVDSMIAQQVDL
                     IFLAPRSEKPLAQAVLKAKSAGIPVILLDRDVDHSIAEPGEDYVCFIGSNFVQEGARA
                     AEWLAETMDGKATIIELEGTTGSSPAIDRQKGFRDYLAGTYVPEDNGGTPASVPAGGF
                     PDMQIIASQTGDFARDEGRQVFETLYQANPTANAVYAHNDEMALGAIAALEAAGKVPG
                     QDVIIVSVDGSRDALQAIIDGKLGATVECNPRFGPKAFETMDKYADGEEIPEMIINPD
                     RFFDATNAEEFIDEAY"
     gene            1916..3103
                     /locus_tag="VGR29_01635"
     CDS             1916..3103
                     /locus_tag="VGR29_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEV2072318.1"
                     /translation="MRITDVTTIKLKYDMPVPMADAVHYMPDRPTLLVQVHTDEGIVG
                     LGEAAVYGGYLESIEALVLGELRQTILNQDPFRVERLWSMMASRAHQRGRGGMLMMGI
                     SGIDIALWDIIGQATRTPLYRLLGGYRDTLDAYASAGFYAGGKDARALAEEVGTYASQ
                     GFKVVKIKVGRNPELMTNMLHDMVRPEYAAVSLEEDIERVRLAREAIGGKVGLAVDAN
                     NAWTPSVALKFMREVESFDIRWLEEPVPTDDIEGSALVAHHLDTPVAGYETETGLPRF
                     RELIARRAVDIVQPDVIWTGGITESRKVAAFAQAYGMPIIPHVFSSAVSTIANMHLIA
                     SLPNGGLLEFDQNPNPLRTELLEEHIAPSSDGTVGLPVRPGLGVTLNQATVDRYRIDR
                     HTP"
     gene            3159..3938
                     /locus_tag="VGR29_01640"
     CDS             3159..3938
                     /locus_tag="VGR29_01640"
                     /EC_number="1.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005472.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-iditol 2-dehydrogenase"
                     /protein_id="HEV2072319.1"
                     /translation="MRLNGKIAIVTGGAQGMGRAISLRYAQEGASVVVADMNLQGAEA
                     VAEEITGAGGKAVAVAVDVRDQDQVQGMVDAAVEHFGGLDILVNNAGVGKIIPFLETT
                     EADWDFMFDINCKGLLWCSQAAARQMIAQGRGGKIVNLASQAGRRGEALVLAYCASKA
                     CVISMNQSIGLALAPHKINVNAIAPGIVDTPFWDEVDKQFAKLLNMEVGEPKRTFIQG
                     IPLGRIEQPEDVTGAAVFLASSDADYITQQTFNVDGGNWPS"
     gene            3970..4308
                     /locus_tag="VGR29_01645"
     CDS             3970..4308
                     /locus_tag="VGR29_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV2072320.1"
                     /translation="MLQRLKKKDPDPREISNAEAAKAYEEQGATFVDVREPDEWAEGH
                     MPGALHIPLGDLVRRENEIPHGGKIVTVCRIGVRSLDAVEILRRTGRKDVKSMAGGMI
                     EWTKNGRAVE"
     gene            4780..5838
                     /locus_tag="VGR29_01650"
     CDS             4780..5838
                     /locus_tag="VGR29_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEV2072321.1"
                     /translation="MVRSSLGPHLHLVWLDQFAYDRAIVGGKGASLSRLIALGASVPK
                     ACALTTSAYTEFAASISLPPRATDACVSELPRIREVIQSTPLPESVAGAIASAYDQFE
                     TLPGVPMSLAVRSSATAEDSANVSFAGLHDTMLGVRSHTALEAAVRQCWASLWSERAF
                     NYRQSRGLETDVAEIAVVIQQMVRADVSFVLFTSDPVTGSGEHLVISASWGLGEAVVS
                     GVVNPDHIVVDRCGCVVDYRVGHKQQMVIADAAPSEGTRQVTVPRALQNMRVMSDDQA
                     SEIAAVGRLVSARLGFEADIEGGFADGKLYLFQARPITTLGVPIRMSPAAASQQTSLA
                     AAWVSNDIPADELQRSSI"
     gene            5997..7514
                     /locus_tag="VGR29_01655"
     CDS             5997..7514
                     /locus_tag="VGR29_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEV2072322.1"
                     /translation="MTATREYDLPIQDVRVRHRNYYYYTSIVMAEPESEEEARQIGEA
                     AEAGIKPELGRLLERWHGEHLPAISAHLDRLKSMEVRGLPRNEMMAILDEADAIHQDL
                     WAIHFRIAHVMLVVIEVFSEFYTDLFDGDDADAHALLVGANSKSVMAGIALSDLAETA
                     RELGLEEVFQETPVENLLPALQATDKGREFLVSLHSFPDVYGLRQDLFDIATPTWRED
                     PSIALSSVRNYLRADHDARAQQIAIARGADEAVAAACEKLALYPEAVREQFEAMLQSA
                     RHGVFLQEEHNFYIDQQGLASLRFFYLRVGQYLKDLGILEAAEDIFMLTVDEVRDALA
                     TDDASQDHGGVRTLVVQRRTEMDIAQTLTPPPFLGDPPSGPPPGDNPLQRTGTKFFGG
                     PPQQAEHANQLKGNPGSRGLRTGVARVARTLEEALHLQPGEILVAITTMPPWTPLFGV
                     AAAVVTETGGSLSHCAIVAREYGIPAVVGAYSATQRITTGQMITVDGSSGVVTLG"
     gene            7678..8238
                     /locus_tag="VGR29_01660"
     CDS             7678..8238
                     /locus_tag="VGR29_01660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2072323.1"
                     /translation="MSVEIGAQQAPAPSRWLLPAIAIGTTVAPLNSTMIAIALPEIQE
                     VFDVSITSTAWLVTLYLVAMAVGQPVGGRLGDLYGRRVYLLGLIWFAVASAGCAFAPT
                     LPWLIGFRIQQALAGALSFPNGAAIIRATFPKARRGVVFGVVGLAAGSAAAAGPPLGG
                     ALVFAWGWSAIFWANLPVIGIAFLLA"
     gene            8350..9129
                     /locus_tag="VGR29_01665"
     CDS             8350..9129
                     /locus_tag="VGR29_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2072324.1"
                     /translation="MRLGYGRISIGAAVASLLLGLAFLRREIRAQAPVVNLKLFRRSR
                     YTFACASISLSNLVRYTTLLALPIFLERVRDHSVQTSGLILAAMSAFAALLGPLGGKW
                     ADLRGYWPPAFWGAIALVTGTLLLTASTVFQEVVLTIVALAVMGFGLGVSGAPVQAAA
                     IEAVPGETAGSAAGIFSTSRYVGSVLGSTVLALVFTREVGPGDTRAFALLFGGLSVAA
                     LAGVFANARVANRHICPKGDPQGYGPEVPATVFVSKGRADP"
     gene            complement(9373..11790)
                     /locus_tag="VGR29_01670"
     CDS             complement(9373..11790)
                     /locus_tag="VGR29_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2072325.1"
                     /translation="MHPLAGAALAPALSPLIGREGELAMAIELLRRDDVRLLTLTGPG
                     GIGKTRLSIEIGCQMAPAFGAGVRVVPLAPVPHSGLVAMTIASTLQIPESGSAEVVES
                     LAFALRDTHLLLILDNLEHVLAAAPLVTNLLVQCPYLKVLATSRASLRVSGEHILPVP
                     PLALPDPGTTTSSEHAAQTAAVQLFVDRAKAVAPFFKVTTTTMPIVSAICQHLDGVPL
                     GIELVAAQSSILPPATLLARIEARLPLPLAGPRDAPVRLRSMRDTIAWSYAYLPVDIQ
                     QLFRLVGVFAGSFGLDAAEAVGERFYKVSKASLEDAVPDGNDDLSPRPAPVHDILGGL
                     ASLVDNSLVQQKEWDGEARFFMLETIRDFAAEELAARGENDAAREAHAAWCLELAEQS
                     QMAAVIPGGEQELSRLEIEHPNMRAALDWLHQREDSDRLLRLATALGEFWFEHNHYRE
                     GREWLELALANSSAAPDTLRAPALVKLGLLLGFQTNTQRAEELFAEGIRLLRVSGDAA
                     AIALALVWQGAIANYLGDHDRAERVLEEALGLAEQIPDASIAASMTARALANLGISAH
                     LQGKLDVAIVRHELALRVCQEHGYVLGAIRSLRDRGDVARDQGDYTASLAYYRECLTL
                     MGDRGDRRIVVDALLGSALAAAVWDQPERAARLVGAADAIADRFGATATVATDQAAHT
                     RVLALIRASMEEQEFETAWTIGREQDVGKAIAEVLAMSPSIAGSRKAVDPSHIHLSGR
                     EEEVLRLVSAGESDREIAEKLFLSVRTVEAHVTRIRTKLGVRTRTAAVTAAIAAGLMD
                     PRAPRSD"
     gene            complement(12239..13252)
                     /locus_tag="VGR29_01675"
     CDS             complement(12239..13252)
                     /locus_tag="VGR29_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEV2072326.1"
                     /translation="MAVDNTVYVDGERLPGPLPLDQTYDVAREQGGLAWISLFQPSGD
                     DLASVAAEFGLHALAVEDTIKAHQRAKLERYGETLFVVLKTARYLDEPEVIEFGELHI
                     FTGKNFVVTVRHNGAPDLAGVRNRIEQQGDFLCRGSEAILYAIIDWVVDGYAPVVAGL
                     ENDIDEIEEEVFSGSPDASRRIYELSREVIEFQRASKPLTGMLNALTAGFDKYGIAPE
                     LQSYLRDAQDHVVRINEQVEGFRQLLQSILSVNLTIVSLAQNEEMQKLTEVSINQNEE
                     VKKISAWAAILFAPTLVGTVYGMNFEHMPELGWLLGYPFALLLMAAVCVGLYLVFSRR
                     GWL"
     gene            complement(13375..13953)
                     /locus_tag="VGR29_01680"
     CDS             complement(13375..13953)
                     /locus_tag="VGR29_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEV2072327.1"
                     /translation="MHKRMVVTVSALLFLLLIAGDNHPSAGAQDGSTGYCAGEAGIEM
                     LKRMNHLRKENRLHPLVLSEPLGIAAEQKAHDMAKHDYVAHVSPDGQDPQELLDEVGY
                     DYNTATGENIAAGHEGAEATFKQWLNSPEHRETMLGENYTAVGIGRAHNAEAKYDWYW
                     VAEFGGEVGKQAEACDRATPVATPVAIKSMGS"
     gene            14171..14611
                     /locus_tag="VGR29_01685"
     CDS             14171..14611
                     /locus_tag="VGR29_01685"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEV2072328.1"
                     /translation="MRFHTTVLSTGKNTTGISMPEDVLAELGGGRRPAVTVTINGYSY
                     RTTVGSSEGRAMISVSSDIRPAAGVAAGDDVDVTVELDTSPREIEVPQALAAAFAEDE
                     AARNAFNGLSNSKKQQLTLPISKAKTDHTRMRNVEKALSTLRGN"
     gene            14977..>15487
                     /locus_tag="VGR29_01690"
     CDS             14977..>15487
                     /locus_tag="VGR29_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072329.1"
                     /translation="MKTPPRSLEADAISAALSAYWNLDSAALTYTPLGFGSHHWIAET
                     TDGEKWFVTIDDLLAAHLGENEDRSFEVLATAFGTAAALRDAAKLAFVIGPIADSSGR
                     WLHRLEGRYSMAVFPFIDVEPSEFGEFQHESDKNDAMRLVGAIHNATAQLSVDRLRKE
                     TLLVPKRTEL"
BASE COUNT         2986 a         4489 c         4756 g         3256 t
ORIGIN      
        1 gaggttgatc tcctggtaga tggtgctgac cccgctcgcc tgcgcctgct gaggcgtccg
       61 gaagtccaca ggttggccat tgaaaacaat cgtccctgaa tccctgcggt atgcgccggc
      121 gaggatcttc atcatggtgg acttgcctgc gccgttctgg ccgacgagcg catggacctc
      181 gcctcgccgt accgtgagac tggcgttcga gagcgcgatc acgccgggga atgccttgga
      241 gatatcctgc atctggagca gaggttctga ctcttgccgg gactccatga gcacactcca
      301 agcatgagaa acagggctat cgcaaccggg acgcatggtt ccgtgaacac ggacggggag
      361 cggcccatcg gaacgctccc tgccctggcg taactcggcc atcagaggtg ctcacggctc
      421 gcttccgcgg ataccgagcc gtgccgcgag gttgccgctg acaatccgct agtaggcttc
      481 gtcgatgaat tcctcggcgt tcgtggcgtc gaagaaccga tccggattga tgatcatctc
      541 cgggatctct tcgccgtccg cgtacttatc catcgtctcg aaggctttcg gcccgaagcg
      601 aggattacac tcgactgtcg cgccgagctt gccgtcgatg atggcctgca gggcatcacg
      661 cgagccatcg actgagacaa taatgacgtc ctggcccggc accttaccag ctgcctcaag
      721 cgctgcgatc gcgccaaggg ccatctcatc gttgtgtgca tacaccgcat tcgcggtggg
      781 gttggcctgg tagagggttt cgaaaacctg tcgcccctcg tcccgtgcga agtcgcccgt
      841 ctgtgaggcg atgatctgca tatcggggaa gccgccggca ggtacggagg ccggggtgcc
      901 gccgttgtcc tcgggaacgt atgtcccggc gaggtagtcg cggaacccct tctggcgatc
      961 gatggcaggg gaggatccgg tggtcccctc aagctcgata atcgttgcct ttccgtccat
     1021 tgtctcggct agccattccg cggcgcgtgc gccttcctgc acgaagttgg aaccaataaa
     1081 gcagacgtaa tcttcgcccg gctcggcgat ggaatgatcg acgtcgcggt cgagcaggat
     1141 gaccgggata ccggctgact ttgccttcag cactgcctgg gcgagcggtt tctcggagcg
     1201 tggagcgagg aagatcaggt ccacctgctg cgcgatcatc gagtcgacgt ccgcgatctg
     1261 cttcgcttcc gatcctccgg catcggtata gacaagctca tggccgagct cttcggcctc
     1321 agcctgcatg cttgccgtct gggctgtgcg ccagggattg ttcatctcca cctgcgagaa
     1381 gcccaccttg tacttggctt tcttctcgag cggtggcggc gcggcgagag ccgttcgcag
     1441 gagcattggc gtcagcatga cagcgcctgc tccggccgcg gaaacctgta ccagccgccg
     1501 acgtgtaatc actcggtcca tgcgttcgat ctcctctact gaatgattcc cttcagcggg
     1561 cgaaatcgcc acgccaagca ctcacagcgc aggttaggca cgtgtccttg cgcgccagaa
     1621 tcagtgacga tttcactggg agtccgcaac agcctgttcc caatgatctt tcctcaatat
     1681 ccgatggata gaccttaccg gcgttaccat tccaccgttg tccaggcgct cctactgatg
     1741 tatatctggt tgtgagttct gagcaaaatt gtagacatag caccttgccc tgtcaaacct
     1801 gggatgactt tccggtattt gtgtccttta tatctcccgt gcgttaccct gtagtccgtt
     1861 tgccgcctgg tggagacgga cggcactgta tatcactcct tgttgggagg tctttgtgcg
     1921 gatcactgac gtcacgacga tcaagctcaa gtacgacatg ccggtcccga tggccgacgc
     1981 cgttcattac atgcccgacc gaccgacgtt gctcgtccag gttcatacgg atgagggcat
     2041 tgtcggcctg ggcgaagccg cggtctatgg cggctatctt gaaagtatcg aggcgcttgt
     2101 tcttggagag ttgcggcaga cgatcctcaa ccaggacccg ttccgggtgg agaggctctg
     2161 gagcatgatg gcttcccgcg cccaccagcg tggtcggggc ggcatgctga tgatgggcat
     2221 cagtgggatc gatatcgctc tctgggacat catcgggcag gcaacacgga cgcctctcta
     2281 ccggctgctc ggtgggtacc gggatacgct tgacgcctac gcatcggcgg gattctatgc
     2341 cgggggcaag gacgctaggg cacttgcgga ggaggtcggt acatacgctt cccaggggtt
     2401 caaggttgtc aagatcaagg tcgggcgcaa tcccgaactc atgaccaaca tgctgcatga
     2461 catggttcgg ccggagtatg cggcggtctc ccttgaggag gatatcgagc gcgtgcggct
     2521 ggcacgggaa gcaatcggag gaaaagttgg cctcgcagtc gatgccaata atgcctggac
     2581 gccctcagta gcactcaagt tcatgcgcga agtggagtcg ttcgacatcc ggtggctgga
     2641 ggagccggta ccgaccgacg acatcgaggg aagcgcgctt gtggcccacc acctcgacac
     2701 accggtagcg gggtacgaga ccgagaccgg tctgccgagg ttccgcgagt taatcgcgcg
     2761 tcgggccgtg gacatcgttc aacccgacgt aatctggacg ggaggcatca cggagagcag
     2821 gaaggtcgcg gcctttgccc aggcgtacgg gatgcccatt atcccgcatg tcttctcgtc
     2881 ggcggtcagc accatcgcga acatgcacct catcgcctcg ttgccgaatg ggggactgct
     2941 ggagttcgac cagaacccga atccgctgcg aaccgagctc ttggaggagc acatcgcgcc
     3001 ctcgtccgac ggcacagtcg ggcttccggt acgtcccggc ttgggagtta cgctcaacca
     3061 ggccactgta gaccgttacc ggatcgatcg acacacacca tagacctatc caccctgcgc
     3121 caactgtcct tcttcactgt tactgagagg agtcggctgt gagattgaac ggcaagatcg
     3181 cgattgtgac cggcggcgcc caaggcatgg gacgcgccat cagcctccgc tatgcccagg
     3241 agggcgccag tgtggtggtg gcagacatga acctccaggg cgccgaggcg gtcgcggagg
     3301 agatcacggg agctggcgga aaggccgtcg ccgtcgctgt agatgtgcgc gaccaggacc
     3361 aggtgcaggg gatggtagat gccgcggtgg agcacttcgg cggtctcgac atcctcgtca
     3421 acaacgcggg ggtggggaag atcatcccgt tcctcgaaac gactgaggcc gactgggatt
     3481 tcatgttcga tatcaattgc aaggggcttc tctggtgcag ccaggcggcg gcgcggcaga
     3541 tgatcgccca ggggcgaggg ggcaagatcg tcaatctggc ttcacaggcg gggcgccggg
     3601 gtgaggccct cgtacttgcc tactgcgcaa gcaaggcgtg cgttatcagc atgaaccaat
     3661 caattggatt ggcactggct ccgcacaaga tcaacgtcaa tgcgatcgcg cccggcattg
     3721 tcgatacgcc gttttgggac gaggtcgaca aacagtttgc gaaactgctc aatatggaag
     3781 tcggtgaacc caagcgcact ttcatccaag gtattcccct tggtcgaatc gagcagcctg
     3841 aggacgttac cggcgcagcc gtcttccttg cttcttcgga tgccgattac atcacgcaac
     3901 agacattcaa cgtggacggc ggcaactggc caagttaatg tccaactcga tcgaggagac
     3961 tatggggtcg tgctgcaacg attgaagaag aaggacccgg atccgcgaga gatctccaat
     4021 gccgaagcgg cgaaagcgta tgaggagcag ggcgcgacgt tcgtggatgt gcgcgaaccg
     4081 gacgagtggg ccgagggaca catgcctggt gcactacaca ttccactcgg ggacctggtc
     4141 cggcgcgaga acgagattcc gcacggagga aagatcgtga ccgtctgccg gattggtgtt
     4201 cgcagcctgg acgcggtcga aatcctccgg cgcacaggcc ggaaggatgt caagtccatg
     4261 gccggtggca tgatcgagtg gacgaaaaac ggccgcgccg tcgagtagtt cggctctttg
     4321 caaggcacag cagggaaacg ggctgtcacg aaggtgactg cccgtttccc tgttcggcag
     4381 actcgctatc cgaacaagac gaagccatct tcttccagcg cgatcaccat ctgcaggccc
     4441 cgcttcgcga cgcgagggaa cctggcgcca ccgaggatgg agtggagatt gctggtcaat
     4501 gcgaccccag tggccgcatg gaatacagcg tcgttgtctc accagggttt cgcctgcagc
     4561 aatgtattga caacatagga agagcgagaa cgatcccaga accctagtgc gtcatgttgg
     4621 cgcaacgtcg ttggtcataa cttttcaaaa agatccattg acaagcaacg gaacttaccg
     4681 gtgcccctct tcgtaatgac tccttagagg cttgtgttcc tatacccgca ggagtacagt
     4741 gttttattac cgttaaatca cgtggaggca gataatcaca tggtgcgcag cagcttgggt
     4801 ccgcacctac atttggtttg gctcgaccag tttgcgtatg atcgtgccat cgttggagga
     4861 aaaggagcca gtctcagccg ccttatcgct ttaggagcat ctgtacccaa agcttgtgcg
     4921 ctgacgactt cggcgtacac cgagtttgcc gcatcgattt ctttgcctcc tcgcgctacg
     4981 gatgcttgtg tgtcggagct tccacgcatc cgtgaggtta tccaatcgac accgcttcca
     5041 gaatccgtgg cgggggctat tgcttctgcc tacgaccagt tcgaaacgct tcccggagtg
     5101 ccgatgtcgc tcgccgttcg gtcgtcagct acagcggagg attctgctaa cgtctcgttt
     5161 gcgggcctac atgacaccat gcttggcgtg aggtctcata cggccctcga ggcagcagta
     5221 cgacagtgct gggcgtctct atggtcagaa cgggcattca actaccggca gtcgcggggg
     5281 ctggaaaccg atgtcgctga gatcgcggta gttatccagc agatggtgcg agcagatgtg
     5341 tccttcgtat tgttcacctc cgaccccgtt accggaagcg gcgagcatct cgtcatatcc
     5401 gcgagttggg gacttggcga agctgtcgtc tctggcgtgg tcaatccaga ccacatcgtg
     5461 gtggatcggt gtggttgcgt agtggactac agggtgggcc acaagcaaca gatggttatc
     5521 gccgatgcgg ctccgtctga gggtacgcgg caggtgaccg tgccccgggc gctccagaac
     5581 atgcgcgtga tgtcggatga ccaggcgtcc gagatcgccg ccgttgggcg attggtctcc
     5641 gctcggcttg ggtttgaggc agatatcgag ggtgggttcg ccgacggcaa gctctacctg
     5701 ttccaggcgc gaccgatcac gacactcggg gttcctatcc gaatgtcacc agctgcagca
     5761 tcgcaacaaa cgtctttggc agctgcatgg gtctctaacg acatcccagc cgacgagcta
     5821 caaaggagca gcatttaatg gcgacatcag tacccagcac tggaacgacg ttcccggctg
     5881 attggtagag tgaggaggat gctggcggca ccttttccct ggaccggatg cataatcctc
     5941 atccgatcag tcccctagct gcatccacca tgggccgtgc attcccgggc ggtttcatga
     6001 cggctaccag agagtatgac ctgccgatcc aggatgtgag ggtacgccac cggaactact
     6061 actattacac ttcgatcgtc atggcagaac cggagagcga ggaggaagcc cggcagatcg
     6121 gtgaagccgc cgaggcaggg attaagcctg agcttggccg gcttctcgag cgctggcatg
     6181 gtgagcattt gccagccatt tccgcgcacc tcgatcggct caaatccatg gaggtccgtg
     6241 gcctgcctcg caacgagatg atggcgatac tcgatgaggc ggacgcaata caccaagacc
     6301 tctgggccat acactttcgt atcgctcacg ttatgttggt ggtcatcgaa gtctttagtg
     6361 agttctatac ggatcttttt gacggggatg acgccgatgc tcatgccctg ttagtggggg
     6421 ctaactcgaa gagtgtgatg gcggggatcg cgctttccga tttggcagag accgccaggg
     6481 aacttggact cgaggaggta tttcaggaga ctcctgtcga gaatctcctc cctgcattgc
     6541 aagcgaccga caaagggaga gaattcctgg tgagcctgca ttccttccct gatgtctacg
     6601 gtcttcgtca ggacctcttt gatatagcaa ctcctacctg gagagaagac ccgtcgattg
     6661 cgctctccag tgttcgaaac tatctccgcg ccgaccacga cgcacgggct caacagatcg
     6721 ccatcgcgag aggcgcggac gaggcagtag ccgctgcttg tgaaaagctc gccctgtatc
     6781 ccgaggcagt ccgggaacag ttcgaggcga tgctgcaatc tgccaggcac ggtgtattcc
     6841 tgcaagagga gcataatttc tatatcgatc agcagggtct tgcctccctg cggttctttt
     6901 accttcgagt cgggcagtac ctgaaggatc tcggcatcct cgaagcggcc gaggatatct
     6961 tcatgttgac ggtggacgag gtgcgcgatg ccctcgcgac ggacgacgca tcgcaggacc
     7021 atggtggcgt ccgcacattg gttgtccagc gccggacgga gatggatatc gcccaaacac
     7081 tgacgcctcc gccgttcctc ggcgatcctc cctcaggacc accaccaggt gataacccac
     7141 ttcaacgaac cgggacgaag ttctttggtg gtcctccgca gcaggcagaa cacgcaaacc
     7201 aactcaaggg gaaccccggt tcccggggct tgagaaccgg ggtggcccgg gtcgcccgca
     7261 ctcttgagga ggcgttacat cttcagccgg gcgagatcct cgttgccatc acgacaatgc
     7321 cgccatggac cccactgttc ggggttgccg ccgccgtcgt cacggaaacc ggcggttcgc
     7381 tgagccattg tgccatcgtc gctcgagagt acggcatccc tgctgtggtc ggggcataca
     7441 gtgctaccca acgcatcacg acaggccaaa tgatcaccgt cgatggaagc agcggcgtgg
     7501 tgacgctggg ataatataca agaagtgtct tgttgattga cttcggcaag ggagcgtgtg
     7561 tcatgccatg tgacagcgct cctttgtttc atttcgatgt tcgaagggcg gctggagctc
     7621 cagtgcgggg cgctattcaa cctggtctct ttaccgggca atcgcaggga ggtgggattg
     7681 agcgtagaaa tcggggctca acaggcccca gcgccatcac ggtggctcct gcccgcgatc
     7741 gccattggga ccacggtggc gcccctgaat tccaccatga tcgccattgc gttgccagaa
     7801 atacaagaag tatttgacgt ttcgattacc tcgacggcat ggttggtcac cctgtacctc
     7861 gttgcaatgg cagttggaca accggtggga gggcgacttg gcgatctgta tgggcggcgg
     7921 gtctatctgc tcgggctgat ctggttcgcg gtcgcctcgg caggttgcgc cttcgcgccg
     7981 acgctcccct ggctcatcgg ctttcgtatc caacaggcgc ttgccggcgc actgagcttt
     8041 cctaacgggg ctgccatcat ccgcgccacc tttcccaaag cgcgtcgcgg cgtggtcttt
     8101 ggtgtcgttg ggctggctgc cggaagtgcc gctgctgccg ggcccccact tggtggggca
     8161 ctggtattcg cgtggggatg gtcggcaatc ttctgggcga atctcccagt gatcggcatt
     8221 gcgttcctcc tggcctagcg aagcctgcct cgcgatgcgt tcggatccat ctcacgcccg
     8281 caattcgact atcagggcac gggactcttt gccctgtctc ttggcagcgt catcgtcatc
     8341 ccttcactgg tgaggctcgg gtatgggcgg atctcgattg gtgctgccgt agcaagcctc
     8401 cttctcggtc tcgcgtttct tcgaagggag attcgagctc aagcgccagt cgtgaatttg
     8461 aagttatttc gccggagccg ttatacgttt gcctgcgcat cgattagcct aagcaacctg
     8521 gtgaggtaca ccacgctgct cgcactaccg atcttcctgg agcgagtaag ggaccacagc
     8581 gtacagactt ccggtctcat cctcgctgcc atgtcggcat tcgcggcatt gctcgggccg
     8641 ctgggcggta agtgggctga cctgcgtggt tactggccac ctgccttctg gggagccatc
     8701 gctcttgtga ccggcactct gctgttgact gccagcacag tcttccagga agtcgtgctg
     8761 acaatcgtgg ctcttgcagt catgggattt ggtctcgggg tgagcggtgc accggtgcag
     8821 gcggcagcaa tcgaggcagt gcccggggag actgcaggtt ctgccgcagg aatcttttca
     8881 acctcgcggt atgtcggaag cgtattggga tccaccgttt tggccctggt gtttactcgt
     8941 gaggtcggtc ccggtgacac tcgagcgttt gcactgctct ttggaggtct ttcagttgcg
     9001 gcattggcag gtgtgtttgc aaatgcgcgc gttgcaaatc gacacatatg ccccaagggt
     9061 gatcctcagg gatatgggcc ggaggttccc gcgacggtat tcgtgtccaa gggtcgagcg
     9121 gatccctgaa cagtcggctc gaagcacaga gccaggtttg ccagcgaacg acggacgcgg
     9181 aggagtacgt cggcatagaa tcgcggcatg tgagcgtctg ctgccgcttt aacggcgacc
     9241 aaatggctct gcgcggagct gtcggtgaag aacggtttga tgcggttgat ggccgatccg
     9301 atctggtgat gaccattggg ggatgaggac ccgggccttg gattttcagc aaccctcctg
     9361 aagctaggct cctcagtccg accggggtgc gcgagggtcc atgagaccgg cggcaattgc
     9421 ggccgtcaca gcggcggtgc gggttcggac tccgagtttg gtgcggatgc gggtgacgtg
     9481 ggcctcgacg gtccgcacac tcaggaagag cttctcggcg atttcccgat ccgattcacc
     9541 ggcactcacc aggcgaagaa cctcctcctc ccttcccgag aggtggatgt gcgaggggtc
     9601 gacggctttc ctggagcccg caattgatgg cgacatggcc aatacttctg cgatcgcttt
     9661 tcccacatcc tgttcccgcc cgatcgtcca tgccgtttcg aattcctgct cctccatact
     9721 cgcgcggatc agcgccagca cccgcgtgtg cgccgcctgg tccgtcgcaa cggtggcggt
     9781 ggcgccgaaa cgatccgcta tggcatcggc tgccccgacc agccgagctg cccgctccgg
     9841 ttgatcccag actgccgcag ccagtgccga ccccaggagc gcgtcgacga caatgcgccg
     9901 atctccccgg tcacccatca gcgtgaggca ctcgcgatag taggcaagtg atgcagtgta
     9961 atctccctgg tcccgcgcta catcgcctcg atcccgtagt gagcgaatgg caccaagcac
    10021 gtatccatgc tcctggcata cgcggagtgc cagttcatgt cgcacgatgg cgacatccag
    10081 tttcccttgc aggtgtgcgg aaatgcccag gttcgctaat gcccgggcag tcatcgatgc
    10141 agcgatggat gcatcgggga tctgctctgc cagcccaagg gcctcctcca gcacgcgctc
    10201 ggcccgatcg tggtctccaa ggtagttggc tatcgccccc tgccatacga gagccagggc
    10261 aatggccgcg gcgtcgcctg atacgcgcaa cagcctgata ccctcggcga aaagctcttc
    10321 agccctttgg gtgtttgtct gaaagccaag gagcaggccg agctttacaa gtgcgggagc
    10381 ccgtagcgta tcgggggcag ccgacgaatt agccagagcc agctccagcc attcgcggcc
    10441 ctcgcggtag tgattgtgct caaaccaaaa ttcgcccagc gcggtggcca gccgaagcag
    10501 tcggtcgctg tcctctcgct ggtgcagcca gtcaagcgcc gcacgcatat tggggtgctc
    10561 gatctccagg cgtgacagct cctgctcgcc tccggggata accgcggcca tttgactctg
    10621 ttccgccagt tccagacacc aggcagcatg cgcctcacga gccgcgtcgt tttcgccgcg
    10681 cgctgccagc tcttccgccg cgaaatcgcg gatcgtctcc agcatgaaga accgcgcctc
    10741 cccatcccac tctttctgct gcaccaagct gttgtcgacg agggaggcaa ggccgccaag
    10801 gatgtcgtgg acaggtgccg ggcgcggcga gaggtcgtca ttgccgtcag gtactgcgtc
    10861 ctctagggac gcctttgata ccttatagaa tctctcaccg acggcttcag cagcgtcaag
    10921 accgaaactt ccagcgaaaa caccaacaag gcgaaagagt tgctggatat caaccgggag
    10981 gtaggcgtag ctccaggcaa tggtgtcgcg catcgagcgg aggcgaacgg gtgcatcccg
    11041 tggtccggcc agtggcaagg ggaggcgtgc ctcgatccgg gcgagcagcg ttgcaggcgg
    11101 aagaatgctg ctttgcgcgg cgacaagctc gattcccaac ggaaccccgt ccaggtgctg
    11161 gcagattgcg gaaacgatcg gcatggtcgt agtggtgacc ttgaaaaacg gtgcaacagc
    11221 ctttgcgcgg tccacaaaga gttgtactgc tgcagtctga gctgcgtgct cggacgacgt
    11281 ggtggtcccg ggatccggaa gggccagggg cgggaccggg agaatatgct ccccgctgac
    11341 tcgcagcgag gcacggcttg tcgccagcac cttgagatag ggacactgaa cgaggaggtt
    11401 agtgacgaga ggagctgcgg cgaggacatg ctcgagattg tcaagaatga gcaacaggtg
    11461 cgtgtcgcgg agcgcaaacg ccagggactc aacaacctct gcgcttccag attcaggtat
    11521 ctggagggtg cttgcgatag tcattgcaac gagtccactg tggggaactg gcgccagggg
    11581 aacgacgcgg acgccggcgc caaaggccgg agccatctgg cacccgatct caatgctgag
    11641 gcgtgttttt ccaatgcccc cgggcccggt gagcgtcagc aggcgaacgt cgtcgcgacg
    11701 cagcaactct atggccattg ccagctcgcc ttcgcgacct atgagcggtg aaagtgcggg
    11761 tgcgagtgcc gctccggcaa gagggtgcaa gtggtctcct cgtctggagt cggtgatcat
    11821 gctcgggcaa aaccagtatt ggacaacgct gcaatgacgg aaaggcagca ccggttgagg
    11881 tcgtgcttat cgaatatttg tgatgccgag cttctgctat cttattctac cctaccgatt
    11941 tgaacgttga agaggaccaa tatttgtcct cgtagcggtg cgagggcctc ctgagcatgg
    12001 aaggtagggg ctccaaacag gaagcggcat ggcgggagtg tcggtgcacc gggttgctcg
    12061 gtataacgat tgagtgggtc cacctactgg tcccaccaag cacccgttgc gtgatgaata
    12121 tccatccatc gaggacagga ggatttgacc accttgttct gatggcctcg taagcagtcc
    12181 gcagcgcatg agcagtccgc ggcgcatgct cactcctcct cgcctttact ggctgagctc
    12241 acagccagcc gcgccggctg aataccaagt agagcccaac gcaaacggcg gccatcagca
    12301 agagggcgaa tgggtagcca aggagccaac cgagttccgg catgtgctcg aagttcatcc
    12361 cgtagaccgt gccaacgaga gtcggggcga acaggatggc cgcccaggcg ctgatcttct
    12421 tgacttcctc gttctgattg atactgactt cggtcagttt ctgcatctcc tcattctggg
    12481 caagactcac gatggtgaga ttgacgctca ggatgctctg cagcaactga cggaaaccct
    12541 cgacctgctc attgatgcgt acgacgtgat cctgggcatc gcgcaggtaa ctctgcaatt
    12601 cgggcgctat gccgtatttg tcgaagccag cggtgagggc gttcagcata ccagtcaggg
    12661 gcttgcttgc gcgctggaat tctatgacct cccgggaaag ctcatagatg cgtcgtgagg
    12721 cgtccgggct tccagagaac acttcttcct caatttcatc gatgtcgttc tccaggcccg
    12781 cgacgaccgg tgcgtagcca tccaccaccc agtcgatgat cgcgtagagg atggcttctg
    12841 agccccggca gaggaaatcc ccctgctgct cgatgcggtt tcgcactccg gcaaggtcag
    12901 gcgcaccgtt atgacgcact gttaccacga agttctttcc cgtgaagatg tgcaactcgc
    12961 cgaactcgat gacttccggc tcgtcaaggt agcgagcggt cttgagaacg acaaagagcg
    13021 tctcgccata gcgttcgagc ttcgcccgct ggtgagcctt gatcgtgtct tcgaccgcca
    13081 aggcgtgaag tccgaattca gccgctactg acgcgaggtc gtctccggat ggctggaaga
    13141 gactgatcca ggcgaggccg ccctgctctc gcgccacgtc gtaggtttga tccagcggga
    13201 gcgggcccgg gagcctctcg ccatctacgt atacagtgtt gtctaccgcc aagaatgcac
    13261 ctctcgttat gcgccgtgac accgcagtgc cacggaagac cgccctgtcg cggctattcc
    13321 aggtactcta accgctgtgg acttacaacg atcttaacgg cgcgctcggg ggcatcagga
    13381 gcccatggac ttgatggcca ctggcgttgc cacgggtgtt gctctgtcgc atgcctctgc
    13441 ttgtttgcct acctcaccgc cgaactcggc tacccaatac cagtcgtact tcgcctctgc
    13501 attatgagct ctcccgatac ctaccgccgt gtaattttca ccgagcatgg tctcacggtg
    13561 ttcgggcgag ttcagccatt gtttgaaggt tgcttcggca ccttcatgac ccgcggcgat
    13621 gttctcgccg gtggcggtgt tgtagtcgta gcccacttcg tccaggagtt cctgcggatc
    13681 ttgcccgtcc ggcgacacat gtgccacgta atcgtgtttc gccatgtcgt gagccttctg
    13741 ctccgcggcg atgcccagag gttcggagag gacaagcgga tgcagccggt tctcctttcg
    13801 aaggtgattc atgcgcttca gcatctcaat cccagcctcc cccgcacagt atccggtgga
    13861 tccgtcctga gccccggcgg aggggtgatt gtccccggct atcaggagca ggaagagtag
    13921 cgcggacacg gtcacaacca tgcgtttgtg cattctcatt ctcctttccc aactggaggt
    13981 tgcagaaccg atgccagtcc tcttcgagca ggactagacg tgtgattctg gatcatcgcc
    14041 ctcagggccg ctcgctacag atcgaggagc gcagtgctca tatatgcttg agctggactt
    14101 gctgtcttta caatggcgga agcagtggac acggacgtta ttcaccccat tcagcattca
    14161 tggaggtcag atgcgtttcc atacgacggt tctctccacc gggaagaata cgacgggcat
    14221 ctcgatgccc gaggacgttc ttgcggagtt gggaggagga aggcgccccg cagtcaccgt
    14281 gacgatcaat ggctactctt atcggacgac ggttggatcg tccgaggggc gggcaatgat
    14341 cagcgtcagt tcagacatac gaccagcggc tggggtggct gccggtgacg atgtcgacgt
    14401 cacggtcgag ttggatactt caccgcgtga gatcgaggta ccgcaagcgt tggctgcggc
    14461 ttttgccgaa gatgaagcgg caaggaacgc attcaacgga ttgtccaaca gcaaaaagca
    14521 gcaacttacg ctgccaatat ccaaggcaaa gaccgatcat acccgcatgc ggaacgtgga
    14581 gaaggcgctg agcacgttgc gcggtaattg aagcgtcagg ggagaggtgc cgcgacggcc
    14641 acttcagctt gtgcggtaca gggacggaga ccggggaaag gacccgccat gtgttttatt
    14701 ccattccttt tggtggccat cgtggtgttc gtcattatcc gcgtctggta atcggcgtcc
    14761 gggcatcagc ccgtctcctg gtatttccgc cgttccccgg ttcgacaaca tgtgcgatgt
    14821 gccaaagaaa tcggcccgcc cttggcggaa tatgttccca gggtttgcat ggtgagcgct
    14881 ccggaccgcc ccggaatgtt ttggtatcag cggaacgggg tctggttttg gccctcaagg
    14941 gttcctaggc attctttggg agaggcgagt ggatcgatga agactccgcc aaggagtttg
    15001 gaggccgatg cgatctctgc tgcactttcg gcgtactgga atctcgattc ggcggctttg
    15061 acatacacgc cgctcggttt cggaagccat cactggatcg ctgagacgac cgatggagag
    15121 aaatggttcg tcaccataga cgacctgctt gcggcgcatc tcggagaaaa cgaggatcgg
    15181 tccttcgagg ttctcgccac cgcgtttggg acggcggcgg cactgcgtga tgccgcgaag
    15241 ctggcgtttg tgatcggacc catcgcggat tccagcggca ggtggctcca tcgtcttgaa
    15301 ggacgctact cgatggcagt ctttccattc atagacgtgg agccgagcga gtttggtgag
    15361 ttccagcacg agtctgacaa gaacgacgcc atgcgtctgg tcggtgcaat ccacaacgca
    15421 actgcccagc tctcggtcga tcggctgcgc aaggagacgt tgctggtacc gaagcggact
    15481 gaactac
//
LOCUS       DASYWM010000042         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_639583, whole genome shotgun sequence.
ACCESSION   DASYWM010000042 DASYWM010000000
VERSION     DASYWM010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639583"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VGR29_01695"
     CDS             complement(<1..763)
                     /locus_tag="VGR29_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV2072330.1"
                     /translation="MENGQEAVSDASAREAGQSPARPHRVVTFGEAMIRLTPPGNERL
                     ERASSLGLTVGGAELNTAVGLACLGVDASWVSALPTTALGRMITRQARANDVDVSGVA
                     WVSEDAGRTGLYFLEEGLDPRPSSVTYDRKDSALARVRPGTFDWASLVDGASLIHISG
                     ITLAISPSVRAEAMEAVRVANQRGVMVSFDLNYRSKLWSEAEARQAFVEIIPRVDVLF
                     ASRGALQTFYGLEGAYEDVLRQAIEKLGVAAVTVNR"
     gene            911..1666
                     /locus_tag="VGR29_01700"
     CDS             911..1666
                     /locus_tag="VGR29_01700"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV2072331.1"
                     /translation="MTPYSQSPQNVTAEAFGDPAVRALVNSLCRTGPLIEESVSIPGS
                     ERRMAVIRPQDIDGLLDQSADDPEQNLPYWAEIWPSGIALAGALLREPELVAGQPVLE
                     LGSGLGITAAVALHLGAQLVATDYAPESIALTKLTCRIHTGQEPETRQVNWRSPDTDL
                     LQEDGSRWPVVLAADVLYEERDIDPVLDVLERILAPGGLVWLAEPGRRPSQLALQRAT
                     GRGWSVASSTWEGEWPDPKDAGVIVRVHQMRRE"
     gene            1867..2976
                     /gene="ugpC"
                     /locus_tag="VGR29_01705"
     CDS             1867..2976
                     /gene="ugpC"
                     /locus_tag="VGR29_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV2072332.1"
                     /translation="MSRVIFDQVTKIYGAGEQPAVNNLSFEIEDEEFLVVVGPSGCGK
                     STAMRMVAGLEEISSGRIIIGDRVVNDLPPKDRNVAMVFQSYALYPHMSVRDNLAYPL
                     KLRKVPKAEREQRVVEAARILDIEQYLDRKPKALSGGQRQRVALGRAIVRNADVFLMD
                     EPLSNLDAKLRVQTRAELVKLHDRVRTTTIYVTHDQTEAMTMGDRIAVMSLGVLQQLD
                     TPQNLYDNPANKFVAGFMGSPAMNFLDVTLEAIDGRLFAVTPGFRMQVPDIQTKNLQG
                     HIGKTVTMGIRPEHISLPERLSGQIADASRIPVTVDVVELLGNEIFVYLTNRGATLTA
                     RMDPDIRLERGQEIEVAAEPDKMHFFDAQTEQTLR"
BASE COUNT          654 a         1050 c          967 g          589 t
ORIGIN      
        1 tccggttcac tgtcaccgcc gcgaccccca gcttctctat cgcctgacgc aggacatcct
       61 catacgctcc ctcaagcccg tagaaggtct ggagtgcccc gcgcgacgcg aagaggacat
      121 cgacacgcgg aatgatctcg acaaacgcct gccgggcttc ggcctcgctc cacagcttcg
      181 agcgatagtt gagatcgaag gagaccatga ctccacgctg gttggcgaca cggaccgctt
      241 ccatcgcctc tgcccggacg gacggtgaga tcgcgagcgt gatgccggag atatggatga
      301 gggaagcacc gtccaccaac gacgcccaat cgaaggtacc cggcctcacc cgcgcaagag
      361 cggaatcctt gcggtcgtag gtcaccgacg acggacgtgg gtccaaccct tcttcaagga
      421 agtacaggcc ggtccggcct gcatcctcgc tcacccacgc cacaccggag acatccacat
      481 cgtttgccct cgcttgccgc gtaatcatcc ggccaagcgc cgtggtcggg agggcagaaa
      541 cccaggacgc atccacccca aggcaggcaa gcccgaccgc cgtgttcagc tccgccccac
      601 cgaccgtaag tccgagcgat gatgcccgct ccaggcgctc attgcccgga ggcgtcagac
      661 gaatcatcgc ctcaccgaat gtcaccacgc ggtgaggacg agcaggtgac tgaccggctt
      721 cccgtgccga tgcatcagac acagcttctt gcccgttctc catctcatgc ctctcctgat
      781 cggtgccgta tgcgtgcccg tcgattcggg atatcccaat cggctccaat tgtatgcacg
      841 atacccgacc agcggtcgtc tcgcggtact ctgagctcag cacctttccg caacctgacg
      901 gagcacgctc atgacgccgt actcgcaatc cccgcaaaat gttaccgccg aagcattcgg
      961 ggaccctgct gttcgggccc tggtgaactc cctatgccgc accggccccc tgatagagga
     1021 gtctgtctcc attccgggca gcgagcgaag gatggcggtg atccgcccgc aagatatcga
     1081 cgggctcctc gatcagagcg ctgacgaccc ggagcaaaac cttccctact gggcggagat
     1141 ctggcctagc ggcattgcgc ttgccggtgc gctgctccgg gaaccggagc ttgttgccgg
     1201 gcaaccggtg ctggaacttg gttccggcct cggtatcacg gccgctgtcg cgctccacct
     1261 cggcgcccag ttggttgcaa ccgactacgc gccggaatcg attgccctca ccaagctcac
     1321 ctgccgaatc catactggcc aggagccgga gacccgacag gtcaactggc gctctccgga
     1381 caccgatctc ctgcaggagg acggctcgcg gtggccggta gtgctcgcag cggatgtgct
     1441 ctatgaggag cgggacatcg acccggtgct ggacgtgctg gagcgaatcc ttgcgcctgg
     1501 cggcctcgtc tggcttgccg agccgggtcg tcgcccatcg cagctggcgc tccaacgtgc
     1561 caccggacga ggctggagtg tcgcgtccag cacgtgggaa ggagagtggc ccgatccgaa
     1621 ggatgcgggc gtgatcgtcc gtgttcacca aatgcggcga gaatgatccg gaaaagcatt
     1681 gacaacggag gccgctgccc ggtacaaaag gcacagcaca tcataaggcg ccgtgatctg
     1741 cgcacagaac gaacaccgat tgcatgattc tggataacgg acatgcatgt cgtagggcgc
     1801 ttcggagcgg gttcccggat ggaccggaag gacctcggct ctgtaatcgg tgaaggagag
     1861 attcgtatgt ctcgtgttat tttcgatcag gtcaccaaga tctacggtgc cggtgagcaa
     1921 cccgccgtca acaatctcag ctttgagatt gaggatgagg agtttctcgt cgtcgtcggt
     1981 ccttccggtt gtggcaagtc caccgccatg cgtatggtcg ccggtctgga ggagatttcc
     2041 tcgggccgaa tcatcatcgg cgatcgggtc gtcaacgacc tgccgccgaa agaccgcaac
     2101 gtcgccatgg tcttccagtc ctacgcgctc tatccgcaca tgagtgtgcg cgacaacctc
     2161 gcctacccgc tgaagctgcg caaggtcccg aaggccgagc gtgagcagcg cgtcgtcgaa
     2221 gccgcacgaa ttcttgacat cgagcagtat ctcgaccgca agccaaaggc cctctctggt
     2281 ggccagcggc agcgcgtggc gctcggtcga gccatcgtcc gtaacgccga tgtcttcctc
     2341 atggacgagc ctctctcgaa cctcgatgcc aagctccgcg tgcagacccg tgccgagctc
     2401 gtgaagctgc acgatcgtgt ccgcaccacg acgatctacg taacccatga ccagacggaa
     2461 gccatgacca tgggtgaccg tatcgccgtc atgagcctgg gcgtgctcca gcagctcgat
     2521 acgccgcaga acctctatga caaccctgcc aacaagttcg tggccgggtt catgggctcg
     2581 ccggcaatga acttcctcga tgtcacgctc gaggccatcg acggcaggct cttcgctgta
     2641 acgccgggct tccgcatgca ggtgcccgac atccagacca agaatctgca gggtcatatc
     2701 ggcaagacag taaccatggg cattcgcccg gagcacatca gcctgccaga gcgactcagt
     2761 ggccagattg ctgacgcctc ccggattccg gtgacggtcg acgtggtcga gctcctcggc
     2821 aacgagatct tcgtttatct caccaatcgt ggcgccacgt tgaccgcccg gatggacccg
     2881 gatatcaggc ttgagcgcgg ccaggagatc gaagtcgcag ccgagccgga caagatgcac
     2941 ttcttcgacg ctcagaccga gcagacgctc cgctaggacg ccgctggtca agtttcgaga
     3001 atcgatcaca gggagagcgc cggtggagac ttccaccggt gctctccctt tttgcagatt
     3061 tcagatggtg ctgagagtga aattacgcgc cacggtgcat cccattaatg agacaccgcc
     3121 tgtgctggtc gacggctgtc atcgccttcc aagaccttgg gcgcaaaaaa cggcctgcat
     3181 ccggacaagg gagggagagg gaaggtgtcc gaaaacaggc gtacgacaag gaaggaaatg
     3241 gcccgcgagt gcatcatgtg
//
LOCUS       DASYWM010000043         5764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_489370, whole genome shotgun sequence.
ACCESSION   DASYWM010000043 DASYWM010000000
VERSION     DASYWM010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489370"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VGR29_01710"
     CDS             <1..730
                     /locus_tag="VGR29_01710"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEV2072333.1"
                     /translation="FLWWPSGDFANSPVFQDLVEIVDRLIVDSAQLGNDASALAQLRT
                     LLDDEDDPWVGDFTWLRLQPWRQLVAQFFDPQEVQPALETIAQVNIAYTPTREDRGSG
                     LAAALLMVGWLASRLNWEVLEPLEQRKAGGWTTPLRSGNSDKKVRHIQVRLSPDQTIE
                     SHFSLRSVEIVSIDDHEGVFRVLRTDKDDLITSSETADAPYVSRVVYARRQTSVEMLG
                     EELQRFGPDPILEDAVRFATRMLP"
     gene            770..1555
                     /gene="pgl"
                     /locus_tag="VGR29_01715"
     CDS             770..1555
                     /gene="pgl"
                     /locus_tag="VGR29_01715"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEV2072334.1"
                     /translation="MSDEQRLIDYAERGTVIVVPDGEALARLAAETLATTSAEAVAES
                     RQALIALSGGSTPKKMGELLASQDYVGRVHWNYLQIFWGDERWVQLEDSQSNAGEAMR
                     TFLTRVPIPDENVHPWETDPDLDPTLSAERYTRMIKEISGVEDSVPVFDLVFLGMGDD
                     GHTLSLFPGTGAIRDTHSLTLAHRVEKLEATRLTFGPVLANAASKVVFLVGGAGKAET
                     LHQVLDGEEQPNLLPSQVIRPESGVLLWLVDDAAAAKLERSNG"
     gene            1548..2240
                     /gene="rpiA"
                     /locus_tag="VGR29_01720"
     CDS             1548..2240
                     /gene="rpiA"
                     /locus_tag="VGR29_01720"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HEV2072335.1"
                     /translation="MDNAERLTRLGIEAAERVQDGMLVGLGTGTTAKAMIDALGDRVK
                     SGLACNGVATSKYTSDLATSLGIPMRKLYDIDRLDLCIDGADEIDPALDLVKGRGGAL
                     LFEKLVARRADRYLIIATDEKLVLRLGTRMPLPVEVIPVGWTHTAESIMELHLRPALR
                     TQDDGSPFITDGGHYIVDCAWPENAEVDPGSLAGALKALTGVVDHGLFIGMATEALTI
                     DLSGEVTEHGRK"
     gene            complement(2323..3537)
                     /locus_tag="VGR29_01725"
     CDS             complement(2323..3537)
                     /locus_tag="VGR29_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEV2072336.1"
                     /translation="MNTIDSGDTAWVLISAALVMLMTPGLGFFYGGLVSRKNVLSTLM
                     HSFFTVALISVVWVLWGYSLAFGPTMGGVIGGLDFFGLNGITPASVTGSIPTLAFVAF
                     QMMFAVITPALISGSFAERKRFKAFVIFTVLWSTFVYSPVAHWVWGGGWIYDLGALDF
                     AGGTVIHITSGVAALVVAMMLGKRKVFGTTETEPHDLTMTVLGAGLLWFGWFGFNAGS
                     ALAANGIAAHAFVVTNVAAASGAIAWLTTSWMRKGHPSILAAACGAVAGLVGITPAAG
                     FVGAWAAILIGLGAGMFCCLTVDLLKSRLKLDDALDVFAVHGVGGIWGALATGLFADA
                     TIGGTDGLFFGDVRQFFVQALAVVVAAAFSALVTFGICKIIDATIGLRVLEEEEVLGL
                     DSSQHGELAYRL"
     gene            3887..4780
                     /locus_tag="VGR29_01730"
     CDS             3887..4780
                     /locus_tag="VGR29_01730"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEV2072337.1"
                     /translation="MTDVTPTIDTGAPELLVDLPSETGEGPLWHEENCCLYWVDIPVG
                     RLFRYDPETGSNETVYRHDGAIGGYTFQQDGSLILFCKGGAILRLIGGETEPVVPEVE
                     ALRESRFNDVIADPEGRIFCGSMPLGGGSAHLYRLDPDGSLTLVFDDLTLSNGMGFSP
                     DLKMMYLSDSNSRQIFRMDYDRKTGEVRNREVLITVPDEGTVPDGMAVDTEGNIWSAR
                     WDGSGLYKYSPEGELLGKVQFPVRKVSSVTFGGPNYDIAYVTTAGGKQRSMEEGMQAG
                     GLFQIDLKTTGRPPFRSRIAM"
     gene            complement(4949..>5764)
                     /locus_tag="VGR29_01735"
     CDS             complement(4949..>5764)
                     /locus_tag="VGR29_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072338.1"
                     /translation="AVTLIVGTADYTINIGDFALNGIALGTFGSIILYQVFRLIPGSM
                     DEDPGATPASAGLDDEYGGAAAGPGVRWNEEERFVSQRRERSGRQERGTRDSRREQRL
                     APQREETFRYDEVDMDDSEATRSRQPPRRPQQRPQPARREPADEFGFGEPPETPPTRR
                     GQQPAQGQRRPRPQEDSRRASSQPFQESGGYVVDDPDAMPRDVRSPRPSRRQEPRESA
                     PVREDIDDFGFDAPMPLPQPPQPPRARRGSPPPQDRRRPRPEGDKEPPRRGGR"
BASE COUNT         1201 a         1805 c         1695 g         1063 t
ORIGIN      
        1 cttcctatgg tggccgagcg gcgattttgc caacagtccc gtcttccagg atctggtcga
       61 gatcgtcgac cggctgatcg tggacagtgc ccagcttgga aatgatgcca gtgcactagc
      121 ccaactgcga acgctccttg atgatgaaga cgatccctgg gtgggcgact tcacctggct
      181 ccgccttcag ccgtggcgac aactcgttgc ccagttcttc gatcctcagg aggtccagcc
      241 ggcccttgag accattgccc aggtaaacat cgcctataca cctacccgcg aggaccgtgg
      301 ctcggggttg gcagccgcgc ttcttatggt cggttggctg gcaagccgtc tgaattggga
      361 ggtccttgag ccactcgagc agcggaaggc cggcggctgg acgactcctc tacggagcgg
      421 caacagtgac aagaaagtgc gccacatcca ggtacggctc tcccctgatc agacaattga
      481 gtcccacttc tccttgagga gtgtggagat cgtctctatc gatgaccacg aaggcgtttt
      541 ccgggtgttg cggaccgaca aggacgacct gatcaccagc tccgagactg ccgacgcacc
      601 atatgtcagc cgtgtggtct acgcgcgacg gcagactagc gttgagatgc tcggcgaaga
      661 acttcagcgc ttcggcccgg atcccatcct cgaggacgcc gttcgtttcg caacccgcat
      721 gctgccatag gaccgacgag cacgcgacat tgaccttgcg ggagagccga tgagtgacga
      781 gcaacgattg atcgactatg cggagcgggg aaccgtcatc gtggtaccgg acggggaggc
      841 gttggcacgt ctcgccgccg agacgctcgc aaccacatct gcggaagccg tagcggaatc
      901 tcgccaggcg ctcatcgccc tctccggcgg ttccaccccg aagaagatgg gcgagttact
      961 tgcttcccag gactatgtgg gtcgcgttca ctggaactac ctccagatct tctggggcga
     1021 tgagcgttgg gttcaactgg aggactccca aagcaacgcc ggtgaggcaa tgcgcacctt
     1081 tctcacccga gtgcccatcc cggatgagaa cgtgcacccg tgggagaccg accctgatct
     1141 cgacccgacc ctctccgccg aacgctatac ccgcatgatc aaggagatca gcggtgtcga
     1201 agacagcgtt cccgtcttcg atctcgtctt cctcggcatg ggcgatgacg ggcatacgct
     1261 ctccctcttc cccggcacag gggcaatccg ggatacccac tccctaaccc ttgctcatcg
     1321 ggtggagaag ctcgaagcca cgcgcctgac cttcggaccc gtgcttgcca atgcggccag
     1381 caaggtcgtg tttctcgtcg gcggggccgg caaggcagaa acgctgcacc aggtactgga
     1441 tggcgaagag cagcccaatc tcctgccctc acaggtaatt cggccagaaa gcggcgttct
     1501 actctggctc gtggacgatg ccgccgccgc caagctggag cgctccaatg gataatgcgg
     1561 aacgcctcac ccggctcggg atcgaagctg ccgaacgcgt acaggacggc atgctggtcg
     1621 gcctcggcac cggcaccact gccaaggcga tgatcgatgc ccttggcgat cgcgtcaaaa
     1681 gcgggctcgc gtgtaacgga gttgcaacgt cgaaatacac gtcggacttg gcaacgtcgc
     1741 tcggcattcc gatgagaaaa ctctatgaca tcgatcggct cgatctctgc atcgatggcg
     1801 cagacgaaat tgatccagcg ctcgacctcg tcaagggtcg cgggggggcg ctactgttcg
     1861 aaaaactggt cgctcggcgt gcagaccgct atctcatcat cgccaccgac gaaaaactgg
     1921 tcctgcgtct gggaacccgc atgccactgc ccgttgaagt catcccggtc gggtggactc
     1981 acaccgccga aagcatcatg gagctccacc tgcgccctgc cttacggact caggacgacg
     2041 ggtcgccctt tatcacggac ggtggccact acattgtcga ctgcgcctgg ccggaaaacg
     2101 ccgaggttga tcccgggtca cttgccgggg cactcaaggc cctgaccggc gtggtggatc
     2161 acggcctctt tatcgggatg gccaccgagg cattgacgat tgacctaagc ggcgaggtaa
     2221 cggagcacgg ccggaaatag caggttcacc ctcatcgtta gggggcaaga ggtgcggttc
     2281 ccgggtggga accacacctc ttgctggacc gtcaatacgt gcctacaacc gatacgccag
     2341 ttccccgtgc tgggaggaat cgagaccgag aacctcttct tcctcaagca cccgcagacc
     2401 gatcgttgcg tcgatgatct tgcagattcc gaaggtcaca agggccgaga acgcggccgc
     2461 tacgaccacc gccagtgcct ggacaaagaa ctggcggaca tcgccgaaga acaatccgtc
     2521 cgtaccgccg atggtggcat cagcgaacaa accggtcgcc agggcacccc agatgcctcc
     2581 aacgccgtgg acagcaaaga catcaagcgc gtcatccagc ttcaggcgag acttcagaag
     2641 atccacggtc agacagcaga acatccccgc gccgagaccg ataaggatcg ctgcccaggc
     2701 tccgacaaaa ccggcggccg gagtgatgcc aacaagacct gctaccgccc cacacgcagc
     2761 tgcgagaata cttgggtgcc ccttccgcat ccaggacgtt gtcagccatg cgatggcccc
     2821 tgacgcggcg gcgacgttgg taaccacaaa cgcgtgcgct gcgatgccgt ttgccgccag
     2881 ggcactaccg gcattgaaac cgaaccagcc gaaccagagc aggccggcgc caaggaccgt
     2941 catcgtgagg tcatgcggct ccgtctcggt cgtcccgaaa actttgcgct tgccaagcat
     3001 catcgcgacg acaagcgccg ctacccccga ggtgatatgg ataacggtgc cgccggcaaa
     3061 gtcgagcgcg ccgagatcat aaatccagcc cccgccccat acccaatggg ccaccgggga
     3121 gtagacgaac gtggaccaca acacagtgaa gatcacaaac gccttgaatc gcttgcgctc
     3181 ggcgaacgaa ccactgatca gcgctggcgt aatgacagcg aacatcattt ggaacgcgac
     3241 gaacgcgagc gtggggatcg accctgtgac ggacgccggc gtgatgccgt tgagcccgaa
     3301 gaagtcaaga cctccaatca cgccgcccat cgtcgggccg aaggcaagcg agtagcccca
     3361 caacacccag acgacgctta tcagcgccac tgtaaaaaag gagtgcatca acgtcgagag
     3421 gacgttcttg cgactgacca gacccccgta gaaaaatccc aggcccggcg tcatcaacat
     3481 gacaagcgcc gccgatatca gtacccatgc cgtatcgcca gaatcgattg tgttcatctt
     3541 gcctgaatcc ctcatcctct accatgcgat cggaagaacc caccacttaa tgagtcctac
     3601 cggcaatcca ggcaccccat cgtgcatgaa tcgattctgc cggacggcgg acggaacaca
     3661 ctacgtacat tctcgacaat tcaggggtga gttatcgtgc attatgcatg aacggataga
     3721 tgatattccg acgcataggg cttttcggca tagatagcat cgcgtgcgat tgtgcttttt
     3781 ggacagcggc cttccgcggc atgagacatg ccgtttgaga ggaagcatgg cgttatctcg
     3841 accgagcccg tacttttctt ggcaagacac cccaaaggag ccacacgtga ccgacgtgac
     3901 acctaccatc gataccggcg ctccggaact acttgttgat cttccaagcg aaacgggaga
     3961 gggtccgctc tggcatgagg agaattgttg cctgtattgg gtcgacattc cggtcggtag
     4021 gctctttcgt tacgatcccg aaaccggcag caatgagact gtctaccggc acgacggtgc
     4081 aattggggga tacacatttc aacaggatgg gtcgctcatc ctcttctgca agggcggagc
     4141 gatcctgcga ctaatcggcg gcgaaaccga gcccgtcgtt ccggaagtcg aggcgctccg
     4201 agagagccgc ttcaacgatg tcattgccga cccagagggg cgaatcttct gcggaagcat
     4261 gccactgggc ggagggtcgg cacatcttta ccggctggat ccggatggat ccctgaccct
     4321 ggttttcgac gacctgacac tctccaacgg aatgggcttc agccctgacc tgaagatgat
     4381 gtacctctct gactccaaca gccggcaaat cttccggatg gattacgacc gaaaaactgg
     4441 agaggtgcgc aatcgtgagg tgctgatcac tgttcccgac gaggggacgg tgccggacgg
     4501 catggcagtg gataccgagg gcaacatctg gtcggcacgc tgggatggaa gcgggttgta
     4561 caagtacagt ccagagggag agctgcttgg caaggtgcag ttccctgttc gcaaggtttc
     4621 cagcgtcacc ttcggtggcc ctaactatga catcgcgtat gtcacgactg ctggaggaaa
     4681 gcagcggagc atggaggaag gcatgcaggc cggcgggctc ttccagatcg acctgaagac
     4741 caccgggcgt ccacctttcc gctcccgcat cgccatgtag gcccaggaca tctggttcag
     4801 gctctcccat cccttggttc gtaacggagc ttggtccgag atcgctccaa ctgagcatgc
     4861 ttttctccat gctcaccggt ggcacataac aaagtggtat ggagcagagg gccgggcata
     4921 tgcccaaccc tctgctcgat attcctcttt accggccgcc cctccgcggc ggctccttat
     4981 caccctccgg ccgcggccgc cgacggtcct gaggaggtgg cgaacctcga cgcgcacgcg
     5041 gtggctgcgg cggttgaggc aaaggcatcg gcgcatcgaa accaaaatca tcgatatcct
     5101 cgcggaccgg cgccgactcg cgtggctcct gtctccgcga cggtcgcggc gaccgaacat
     5161 cgcgcggcat ggcgtcagga tcatcgacta cgtagccgcc actctcctgg aacggttgtg
     5221 aggaggcgcg acgggaatcc tcttgcggtc gaggccggcg ttgaccttgg gccggttgct
     5281 gcccacgccg cgtcggcggc gtctctggcg gctcaccgaa tccgaactca tcggcaggtt
     5341 cacgtcttgc tggctgtggc cgttgctgcg gccgtcgcgg aggttgcctc gaacgcgtcg
     5401 cctcagaatc gtccatatca acctcgtcgt accggaaggt ctcttcccgc tgcggagcca
     5461 gtcgctgctc ccggcgacta tcgcgtgtgc cgcgttcctg acgcccactg cgctcacggc
     5521 gttgagagac aaagcgttct tcctcgttcc acctgacgcc cggcccggcg gccgcgccgc
     5581 cgtactcgtc atcgagcccg gcactggctg gggtcgcacc tgggtcttcg tccatagaac
     5641 ctggtatcag gcgaaagact tggtacagga tgattgagcc gaaggtgccg agcgcgatcc
     5701 cgttcagtgc aaaatcaccg atgttgatcg tatagtccgc cgtgccgacg atcagcgtca
     5761 cagc
//
LOCUS       DASYWM010000044         6200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_583365, whole genome shotgun sequence.
ACCESSION   DASYWM010000044 DASYWM010000000
VERSION     DASYWM010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583365"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VGR29_01740"
     CDS             <1..1094
                     /locus_tag="VGR29_01740"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEV2072339.1"
                     /translation="PNKKIRVAVVFGGRSDEHDVSLRSARTIMDALDPDRYEVVPMGI
                     TREGRWIAGEDPMAALIEVSPMFHLGEGEQKVEPTGERSIVPVVGAEALIPGGVTDSV
                     DVFFPALHGPMGEDGTIQGMLELAGLPYVGSGVLGSATAMDKAMTKVVMAQAEIPQLP
                     WMLVLRRDWERNPEGVEAQIAEEIGFPCFVKPANLGSSVGITKAHNAMELTEGLREAA
                     HFDRRLVVEKGVVGRELEISVLGTDDPIVSVAGEIRPRGEFYDYNAKYIDDTAELIIP
                     AELEPEVQNQLEEYAARAFTALDLAGLARIDFFLEDGTDQIYLNEVNTMPGFTSISMY
                     PSLWAASGLELGDLVDRLIRLALERHGEK"
     gene            1376..2662
                     /gene="ftsZ"
                     /locus_tag="VGR29_01745"
     CDS             1376..2662
                     /gene="ftsZ"
                     /locus_tag="VGR29_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV2072340.1"
                     /translation="MQSRGQNPARGQQQGRGQSAPRPAPQQNQPQNDGPASAGFSDRP
                     SSRWSSSESLANAFARIKVVGVGGAGGNAINRMVNSGVDGIEFVAVNTDAQALMTSEA
                     TIAIRIGDKLTKGLGAGGRPEIGERAAEESSESLAEVMRDCDMVFIAAGMGGGTGTGA
                     SPTVARLAKDAGALTVAVVTKPFEFEGGRRRRAAEEGVAALKEVVDALITIPNQRLLH
                     MVDPKTTVTEAFQIADDVLRQGIAGISDLITKPGVINLDFADVKTIMQDAGSALMAIG
                     YGEGPDRCVNAAREAIESPLLEMNIQGAKGVLYNISGGSNLTLFETSEAAEVIRAAAD
                     DDAEIIYGTSIEEALSDAVMITLIATGFDERDVMDAYAFDTGSRMPQQEDRFGGRQQQ
                     APSRQPEPRGQGGGQPVYPDDDWESESSIIRFLRER"
     gene            2755..3246
                     /gene="nrdR"
                     /locus_tag="VGR29_01750"
     CDS             2755..3246
                     /gene="nrdR"
                     /locus_tag="VGR29_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEV2072341.1"
                     /translation="MRCPLCKAEDTRVIDSRDVNNGESVRRRRECGECHHRFTTYERV
                     EAPALIVVKRDGRREEFSPEKLRQKLRVALTKRPIGEDVLDSIIRRVESEMRAMGKKE
                     VSSQVIGERVLSELKALDHVAYIRFASVYRQFADIDELRREVEGLSGPDTVPGSLKDP
                     KQG"
     gene            complement(4093..4419)
                     /gene="trxA"
                     /locus_tag="VGR29_01755"
     CDS             complement(4093..4419)
                     /gene="trxA"
                     /locus_tag="VGR29_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV2072342.1"
                     /translation="MSKPVNVTDATYQQEVIEADKPVLVDFWAPWCGPCRMVGPVLEE
                     IASEEAENLKIVKVNVDENQQYAGQLGVFNIPTMVIYKGGQPVDKLMGALPKKQIMDR
                     VKPHLS"
     gene            4606..5697
                     /locus_tag="VGR29_01760"
     CDS             4606..5697
                     /locus_tag="VGR29_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV2072343.1"
                     /translation="MPYSGIGTYLRGLLDALQADADVLGVDVQLLSPSDGRFLRPGTR
                     AHRFAWDAAGVTGQLLWTAGPRPQVLHLPQMSAPLWSPTPMVVTIHDVIPFVLKDYRS
                     SRTMRVYLALMARSARRGQRVIVPSESASIDVQQVLGIHADKIRVIPEAAGPDLVPCR
                     DGEAAAKVRELWGLEGRYLFNIGGFDRRKNLPMLIEAFAAARPSLPEKVKLVIAGAPH
                     TANSLVFPPLEPLIRKHGLAESVILTGRVSDDERRWLYQAAHAYITPSIYEGFGLTPL
                     EAMACGVPAIVANRTSLPEVVEKAGLVVEPEVDDLAASIRVLMTDDDLRARLGVASLE
                     RAGTFTWSTAARSTAAVYHEVISARQLQT"
     gene            complement(5796..>6200)
                     /locus_tag="VGR29_01765"
     CDS             complement(5796..>6200)
                     /locus_tag="VGR29_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072344.1"
                     /translation="LTQAEQQVQLNSLNPNAITQTIQVDLNGVLNGDEDALAEARNAL
                     DSVLAPYLQGQSCRIGFVNISSRAPDIGQGIQLSDRIAELIEDEFPQLIPQPADGGSP
                     QIASESIALPNTTPTGEVELQLFLSSGCDAAG"
BASE COUNT         1195 a         1759 c         2009 g         1237 t
ORIGIN      
        1 tccccaacaa gaagatccgc gttgcagtcg ttttcggcgg acggtctgat gaacatgatg
       61 tctccctgcg ttctgcccga acgatcatgg atgctcttga ccccgatcgt tacgaggtcg
      121 tcccaatggg gatcacccgg gaaggacgct ggatcgcggg agaagacccc atggcggccc
      181 tgattgaggt ctcgccgatg ttccatctcg gtgagggtga gcagaaggtg gagcccaccg
      241 gtgagcgctc aatcgttccg gtcgtcgggg ccgaggcgtt gataccgggt ggcgtgacgg
      301 attcggtgga cgttttcttt cccgcgctcc atgggccgat gggcgaggac ggaacgatcc
      361 agggcatgct tgagctcgca ggccttccct atgtgggtag tggcgtgctt ggctcggcga
      421 cggcgatgga caaggccatg accaaggtcg tcatggcgca ggcggagatc ccccagctgc
      481 cgtggatgct ggtgctgcgt cgcgattggg agcggaaccc ggagggtgtc gaggcacaga
      541 tcgccgagga gatcggcttt ccctgcttcg tcaagccggc aaacttgggt tcaagcgtag
      601 gaatcaccaa ggcccataac gccatggagc ttaccgaggg acttcgggaa gcggcacatt
      661 ttgaccggcg gctggttgtc gaaaaaggtg tcgtcgggcg agagctcgag atctcggtac
      721 tcgggactga cgaccccatc gtctcggtcg cgggcgagat ccgtccccgc ggcgagttct
      781 acgactacaa cgccaagtac atcgacgaca ctgccgagct cattattcca gccgaactgg
      841 agccagaggt ccagaaccag ctcgaggagt acgccgcgcg ggcgttcacc gcactcgacc
      901 tggccggtct cgcccgaatc gactttttcc tcgaggatgg taccgaccag atttacctca
      961 atgaggtcaa cactatgccc ggatttacct ctatcagcat gtatccatca ctttgggcgg
     1021 cgagtggcct ggagcttggc gacctggtcg atcggctgat ccggctggcg ctggaacggc
     1081 acggagagaa atagcagcac attcgtacgc gcgttcgttc gcagccatgc aggtacttaa
     1141 gcagaatcaa tggggcaagg ggagggtgta tgtgcatgct atacttcgcc atgattgcca
     1201 gatggtgcgc tttttcctta tgttgttgca ccctgtgttg catattgtgg tcacctgtta
     1261 cacacgtgca atgagtattg agagttttga cagtaactgg ggcagtcgcc gcctgcgagt
     1321 gcgggtcgaa tcagggtggc agctaccgtt gtgaaacatg gatggtgaag ggactatgca
     1381 atcccgaggt cagaatcccg cacgaggaca gcagcagggg cgaggacaat ccgctccacg
     1441 ccccgcgccg cagcagaacc aaccgcagaa tgatggcccg gccagcgcag gcttctccga
     1501 caggccgtca tcgcgctgga gctcgtcgga atccctggcc aacgctttcg cccgcatcaa
     1561 ggttgtgggc gtagggggtg cgggtggcaa cgccatcaac cgcatggtga actctggcgt
     1621 cgatggaatc gagttcgtcg ccgtcaacac cgatgcccag gcactgatga ccagcgaggc
     1681 gacgatcgca atccgcatcg gagacaagct caccaagggc ctgggggccg gtggacggcc
     1741 cgagatcggt gagcgtgccg ccgaggagag ctccgagagc cttgccgagg tcatgcgtga
     1801 ttgcgacatg gtgttcattg cggctggcat gggcggtggt actggaaccg gagcgtcccc
     1861 gaccgttgcc cgtctcgcga aggacgctgg tgcactgacg gtggccgtcg tgaccaagcc
     1921 gttcgagttc gagggcggac gtcgacgccg ggcggcggaa gaaggtgtcg cggccctgaa
     1981 agaggtggtc gatgccctga tcaccatccc gaaccaacgc ctcctgcata tggttgatcc
     2041 gaagacgacc gtcactgaag ccttccagat tgcagatgat gtgctgcgac agggtattgc
     2101 cggtatctcg gacctcatca cgaagccggg cgttatcaac ctcgacttcg ccgatgtgaa
     2161 aacgatcatg caggatgccg gatccgcgct gatggcgatt ggctatggtg agggccctga
     2221 ccgctgcgtg aacgcggctc gggaggcgat cgagagcccg ctgctggaga tgaacatcca
     2281 gggcgccaag ggtgtgctct acaacatctc cggcggcagc aacctcacgc tcttcgagac
     2341 gagcgaagca gcggaggtta tccgggcggc ggccgatgac gatgccgaga ttatctacgg
     2401 tacatcgatc gaagaggcgc tgagcgatgc cgtcatgatc acgctgatcg ccacgggatt
     2461 cgacgagcgg gacgtcatgg acgcctatgc gttcgatacg ggcagccgca tgccacagca
     2521 ggaggatcgc ttcggcggcc gacagcagca ggcgccctct cgtcagccgg agccgcgcgg
     2581 ccaaggtggt ggtcagccgg tctacccgga cgatgactgg gagtcggaat cctcgatcat
     2641 ccggttcctt cgggagcgct aggcagccgg agactgagca gagcaacgag atgcccgcgc
     2701 gagtatcgtg cgggcatcgc tgtatcaccc ggagacggag ggagaggatt cccagtgcga
     2761 tgtccacttt gtaaagctga agatacgcgg gtgatcgaca gccgcgatgt caacaatggt
     2821 gaatccgtgc gccgccgccg cgaatgcggg gagtgccacc accgcttcac tacctatgag
     2881 cgggtggagg cgccggcgtt gatcgtcgtg aaacgggatg gacgccgcga ggagttcagt
     2941 ccggagaagc tgcgacagaa gctgcgggtt gcactgacca agcgaccgat cggtgaagac
     3001 gtgctggata gcatcatccg gcgagtcgag agcgagatgc gggcgatggg caagaaagag
     3061 gtgtcgtcgc aggtgatcgg ggagcgcgtg ctatctgagc tgaaggcgct ggatcacgtg
     3121 gcgtatatcc ggtttgcatc ggtctatcgc cagttcgccg atattgatga gctgcggcgc
     3181 gaggttgaag ggttgtcagg tccggacacg gttccagggt cgttgaaaga tccgaagcag
     3241 gggtagggca cccgttcagg tgacggtgac cgagtgcctg cagcgaatgg atcgcttcgc
     3301 tattcgccat tcgtcccatc ggctcacccg gggacttgcg gtcccagaga cattgagggt
     3361 gcgctacgga tgtgaacgaa gccgccagaa tacgggtgaa ggtggccaag tgaaataatg
     3421 tggccggtga cagccttgcc ggatttcgtt ggtatcgccg ggttttttag gctgccaagg
     3481 gatggctgaa ttcctccagc ctgccctatc ggcagtggtc gtcaggtggc cctcatggcc
     3541 atctgggcca cttggcagtg tgaggctggt tcatcgacgg tgggtaatta cgtccaagac
     3601 gatcatgatc acgctctatt gcgctcgccg ttgggatcaa gtgcccttcg agacgtggcg
     3661 aatgcaggag cccttattta gatatacgca tcgaggaagc tggtatgcgc tacggatcat
     3721 gaacgaacgt tcctcatgtc tcatccgcat ttgcccacac cgcttcgcgc aggattcttc
     3781 cccttcgacc tcgctcaggg tcagaatgac ggcatgtttg atgtgagaat gtttcccgca
     3841 tcgaggttag cgtgtagtcc gctggcccat gccgaggccg cagggcgaga agcgaaaggc
     3901 tcgctgctgc ttttcgcctt ttcgggttca ccttgcacca ttcggggtat ggtccctgca
     3961 cgacatcagg tacgcgatgg aggaaagcgg aaatatgtga gggcatccag gtagggcggc
     4021 gagcgctcat gcgaaaacgc ccggccgcgc gtgcagccgg gcgttttcgt tcgtgctgga
     4081 tagtgatctg gactaactga ggtgcggctt gacgcggtcc atgatctgct tcttgggcag
     4141 tgcgcccatc agcttgtcga ccggctggcc acccttgtag atgaccattg tcgggatatt
     4201 gaagacaccg agttggccag cgtactgctg gttctcgtcg acattgactt tgacgatctt
     4261 caggttctca gcctcttcag acgcgatctc ttcgaggaca gggccgacca tacggcatgg
     4321 gccgcaccac ggagcccaga agtccacgag caccggctta tcggcctcga tgacttcctg
     4381 ctggtaggtt gcatcggtaa cgttgactgg cttggacata tgtgttcctc cacaatactt
     4441 cacacacggt tgttactatc taaatgatag cacaccatgc aaatgatccg gttgcagctt
     4501 cgcggatgcc ttctcccgtc tatactacgt cccgatatga agcacggcaa cacaggcccg
     4561 gggaccgctc cgctcaccat agccatagat ggcaattttc tcgccatgcc atattcgggc
     4621 attggcacct accttcgtgg cctccttgat gcgcttcagg ctgatgctga tgtgctcggt
     4681 gtagacgttc agttgctttc cccgtccgac ggccgcttcc tgcgccccgg cacgagggcg
     4741 catcgcttcg cgtgggatgc ggcgggtgtg accgggcagc ttctctggac ggctggtccg
     4801 cgcccgcagg tgctgcacct gccacagatg agtgcccccc tgtggtctcc cacaccaatg
     4861 gtggtgacca tccatgacgt catcccgttt gtcctgaagg actatcgatc cagccggacg
     4921 atgcgggtct atctcgcgtt gatggcacgg tcggcccgga gaggccagcg cgtcatcgtg
     4981 ccgtctgagt cggccagtat tgacgtccaa caggttctcg ggatccatgc agacaagatc
     5041 cgggtaatcc cagaggcggc aggcccggac ctcgtcccat gccgcgatgg cgaggccgcg
     5101 gcgaaagtgc gagagctctg gggcctggaa ggccgctatc tcttcaatat cggcgggttc
     5161 gaccgccgga agaatctccc gatgctgatc gaggctttcg cagccgcaag gccgtccttg
     5221 ccagagaagg tgaaactggt gatagccggc gcaccccaca cggctaattc gctcgtcttc
     5281 cccccgttgg agccgctcat ccgcaagcat ggactggcgg agagcgtcat cctgaccggt
     5341 cgcgtttcgg acgatgaacg gcgctggctc taccaggccg ctcacgccta catcacgcca
     5401 tcgatctacg agggctttgg gctgacacct ctggaggcaa tggcgtgcgg cgtacccgcg
     5461 attgtggcaa accggaccag cctgccagag gtcgtagaaa aagccggtct ggttgtcgaa
     5521 ccggaggtag acgacctggc ggcgtcgata cgggtactga tgaccgacga cgatctacga
     5581 gcgcgtcttg gtgtcgcgag cctggaacga gccgggacgt tcacatggtc gaccgccgcg
     5641 cggtcgacag cggcggtcta tcacgaggtg atctcagccc gtcaattaca gacataagct
     5701 tctgagcgtg cgaatccggc gggaatccag agacacggta gccaggggca agcggagaaa
     5761 cacgacgctt gcccctggct actgttccat ctgccttatc ccgccgcgtc acatcccgaa
     5821 ctgaggaaga gctgtaactc gacctcgccc gttggcgtgg tgtttggcag ggcgattgat
     5881 tcacttgcga tctgcggcga tcctccgtcg gcaggctggg ggatcaattg cgggaactca
     5941 tcctcaatca gctctgcaat acggtcggaa agctggatgc cctgtccgat gtccggggca
     6001 cgggaggaga tgttcacaaa cccgatccgg caggactggc cctgcaggta gggtgcgagc
     6061 acgctgtcga gcgcgttccg ggcctcggca agcgcgtcct cgtcgccgtt cagtacaccg
     6121 ttgagatcga cctggatcgt ctgggtgatc gcattggggt tcagggagtt gagctggact
     6181 tgctgctcag cctgggtgag
//
LOCUS       DASYWM010000045         4169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_339401, whole genome shotgun sequence.
ACCESSION   DASYWM010000045 DASYWM010000000
VERSION     DASYWM010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339401"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2233)
                     /locus_tag="VGR29_01770"
     CDS             complement(<1..2233)
                     /locus_tag="VGR29_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEV2072345.1"
                     /translation="MYDPLAGRFDPTLSTRAVFTPADAPSLLYGTDMTEGVIAVEPAG
                     SNGVVLYKRDGRNRTTTERRVTQPWLISSDPAAFAGKDDASIKELRGDHPYRFLVTFE
                     SWESYRNATSQGRPNNTSILGPPSQVTQYLVRSGLTLFKGMDFPELRRMQIDLETLSL
                     DPNSEDAGIIMISLRQCNHEEVLILESTEADLLERFNRTLRALDPDVIEGHNVYRFDF
                     PYLVDRARHTGVTLRLGRDGSMPRIVDGGRRQPSVFVNGRHVIDTYVQIQRFDVQGNL
                     TRYGLKDVIRQLSLEREDRVFVDRASIAKMWRNGSRERETLAAYALDDVRDVDMLSRV
                     IMPTEFYQTQMLPTTYQRSSTTGTGRKIDDLMLRSYLAAGHSLPRAQASRPYPGGYVE
                     LVQAGAFERVVKCDVESLYPSIMLAEHIAAQSDVLETFPLLLEDLTKRRIEAKRRVQS
                     AKGEERATWQGLQGSFKILINSFYGYLGFARGSFNDYDAAERVTLEGQSLIQDVVRHL
                     QDLGAEPIEVDTDGVYFSPPSNVQTPDEEEAFIHRISARLPEGIRLAHDGRFRHMLSL
                     KQKTYALIDYEGSLALTGSALRSRALEQCFQQFIRDAARGFMEEDREAVKVMYFSVAE
                     AIQKRTLSVHDISQWTMLKQETFNSRTRLKQLLESIPGRWRFGERVEVYEREDGTLGL
                     AEDYAHDENTRALLKRLRDVADRFLPLFEDQAEFDATFPPIKLTTDLEQARRRGVVRQ
                     LGLF"
     gene            2581..3033
                     /locus_tag="VGR29_01775"
     CDS             2581..3033
                     /locus_tag="VGR29_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072346.1"
                     /translation="MHMAPLLSAFGHVDALIPVPLHASKAKQRGYNQSELLAEDVSKL
                     TGVPVLTVLRRARKTISQTSLSGHDRKLNVTGVFESDATWVPPAGGRYVLIDDVRTTG
                     STLNACAEALRPLQPVMVGALTFALDMHRDRLAELREYRVGLSSGPIP"
     gene            complement(3014..>4169)
                     /gene="dxr"
                     /locus_tag="VGR29_01780"
     CDS             complement(3014..>4169)
                     /gene="dxr"
                     /locus_tag="VGR29_01780"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEV2072347.1"
                     /translation="HWVDHHGGFVMPGIALLGSTGSIGEQTLDVIATMEDRFRVVTMA
                     AGLREDLLSRQVARFRPDMVVSAHESPDGNRPTPQQLIEAATHPDVDIVVVATSGHDA
                     IPAVIAALEAGKAIALANKEAIVCAGELIMPLAKRLGQVIRPVDSEHSAIWQSLQAGR
                     SEDVRRIILTASGGPFRSWSRDQLRDVTVEQALKHPNWSMGGKITIDSATLMNKGLEL
                     IEARWLFDTDYEHIDVVIHPEQIIHSMVEYNDRSTIAQLSPPDMRLPIQYALTWPEHA
                     PSEFAPLDFTTLSSLSFEVPDLNRFPALRLARQAGEAGKTCPTVLSATDEVAVEAFRS
                     RQIAFTNIPVLIDSVLQRHEPREVVHMDVVLEADRWARAEARQLLKVWGH"
BASE COUNT          831 a         1165 c         1219 g          954 t
ORIGIN      
        1 caaacaaccc caactgccgt accacccccc gccgcctcgc ctgttccaga tccgttgtca
       61 gcttgatcgg tgggaacgtc gcgtcgaact cggcctgatc ctcgaagagg ggcaggaatc
      121 gatcggcaac atcccgaagg cgtttgagga gcgccctggt attctcgtcg tgggcataat
      181 cttctgcaag ccccagagtg ccgtcctctc gctcgtagac ttccactcgc tctccgaagc
      241 gccacctgcc tggtatcgat tcaaggagtt gcttgagcct ggtcctgctg ttaaatgtct
      301 cctgcttgag catggtccac tgactgatat catgcacgct caatgttctc ttttggatcg
      361 cctctgccac ggagaagtac atcaccttga ctgcctcccg gtcctcctcc atgaatcctc
      421 gtgctgcatc tctgatgaac tgctggaaac attgttccag ggcacggctg cgcagagcgc
      481 taccagttag cgccagtgac ccttcatagt cgatgagcgc atacgtcttt tgcttcaggg
      541 acagcatgtg cctgaaccgg ccgtcgtggg ccaggcggat tccctctgga agccgggcgc
      601 taatgcgatg tatgaaggct tcttcctcgt ccggtgtctg gacgttgctt ggtggggaga
      661 agtagacgcc gtcggtgtca acctcgattg gttcagctcc cagatcctgg aggtgtctga
      721 cgacgtcctg aatgagactt tgcccttcga gggttacgcg ctcggcggcg tcgtagtcgt
      781 taaagctgcc ccgcgcgaag ccgaggtatc catagaatga attgatcaag atcttgaagc
      841 tgccctggag accttgccat gttgcccgct cttcaccctt agcgctttgt acgcgccgtt
      901 tggcttcgat gcggcgtttg gtgagatcct caagcagaag cggaaatgtt tcgaggacgt
      961 ccgactgagc ggctatgtgt tcggcgagca taattgaggg atagaggctt tcgacatcgc
     1021 atttcactac gcgctcgaac gctcctgcct gtaccagctc aacatacccg ccgggatacg
     1081 gacggcttgc ttgcgctcgc ggaaggctgt gtcctgcagc gaggtaggat cgaagcataa
     1141 ggtcatcaat cttccgcccg gtacctgtcg ttgatgagcg ctggtaagtc gttgggagca
     1201 tctgcgtttg gtaaaactcg gttggcatga tcacccgtga aagcatgtcg acgtcacgaa
     1261 cgtcgtccag cgcatacgct gccagcgtct cgcgttcgcg ggatccgttt ctccacattt
     1321 tggcgataga ggcacgatcg acaaagacac ggtcctcgcg ttcgagggat agctggcgaa
     1381 ttacatcctt gaggccatag cgtgtgagat taccctggac atcgaagcgc tggatctgca
     1441 cgtatgtgtc gatgacatgg cggccattaa caaagaccga tggctggcgg cgcccaccgt
     1501 ccacgattct cggcatggag ccatctctcc caagccgcag ggtgacgccc gtatgtcgcg
     1561 cccggtccac aaggtacggg aagtcaaatc ggtatacgtt atgcccctcg atgacgtctg
     1621 gatcaagtgc acgtaaggtg cggttgaatc gctccagaag atccgcttcc gtcgattcca
     1681 ggatgagaac ttcctcgtgg ttgcactgtc gaagcgagat cataatgatg ccggcgtctt
     1741 ccgaattggg atccaggctg agggtttcca ggtcgatttg cattcgccgc agttcgggga
     1801 aatccattcc cttgaagagc gtgaggccgg agcggacgag atactgggta acttgcgacg
     1861 gtggccccaa aatcgacgta ttattgggtc ttccctgtga cgttgcatta cggtaggatt
     1921 cccaggattc gaaggttaca aggaaccggt atggatggtc gccgcgtagc tccttgatag
     1981 atgcatcgtc tttacctgca aaggctgcag ggtccgacga gatgagccag ggttgtgtca
     2041 cccggcgttc agtggttgtg cggttgcggc catctcgttt gtacagcaca accccgttgg
     2101 agccggcggg ctctactgcg atgactccct ctgtcatgtc cgtaccgtag aggagggaag
     2161 gtgcatcggc aggtgtgaat actgcacgcg tggagagtgt cggatcgaag cgacctgcaa
     2221 gcggatcata catacggaag tccgtcctcc taggggcata gtatgactat aagcatacga
     2281 cgcattggcc gcacggctct cgccactgtg gtagaagcgg tttacccgaa gatctgtgcg
     2341 ggttgtggca tgcgaggcgt atggctttgc aattactgcg agtcgactgt gccttcgtgt
     2401 aacctgccaa tatcctgttc gcgatgtggc gtaccgacct tgcgaggcag gtgtgggtgt
     2461 gctgacctgg accctgtgat cgcatgcgcg cgatcagcgt ttgattacaa tgggtggaca
     2521 gccacggccg tcaagcgcct gaagtatcag ggggagtcgt cacgagcgga gcatctggcc
     2581 ttgcacatgg ccccgctgct ttcggcgttc ggacatgtcg acgcgctgat tccagtgccc
     2641 cttcacgcct ccaaagcgaa gcagcgtgga tacaatcagt ccgaattact tgctgaggat
     2701 gtcagtaagt tgaccggggt cccggttctg accgtccttc gccgagcgcg aaagacgatc
     2761 tcccaaacgt ccctttcagg ccatgatcgg aaactgaatg tcactggtgt tttcgagagt
     2821 gacgcgactt gggtaccacc cgcgggtggg cgctacgtac tcatcgacga tgtccggaca
     2881 acgggatcta cgttgaacgc ctgtgcagag gcgttgcgac cgctccagcc ggtgatggtg
     2941 ggcgctctga ctttcgcgct tgatatgcat cgcgaccgcc tagcggaact tcgcgagtac
     3001 cgggtcggct tatctagtgg ccccatacct tgagcagttg ccgcgcctca gcacgcgccc
     3061 aacgatctgc ctccaacacc acatccatat gcacaacttc cctgggctca tgacgctgca
     3121 aaaccgaatc gatcagcact ggtatgttgg taaaagcaat ctgacgagag cgaaacgctt
     3181 ctacggcgac ttcatcggtg gcactcaata ccgtcgggca cgtcttgcca gcttcgccgg
     3241 cctgacgggc gagacgtaga gccgggaatc ggttcaagtc tggtacttcg aacgacagcg
     3301 atgacagtgt cgtgaaatcc aacggtgcga actctgacgg agcgtgctcc ggccatgtca
     3361 gcgcatactg gatcggcagc cgcatgtcag gagggctcag ctgagctatt gtgctccggt
     3421 cgttgtactc caccatcgag tgaatgattt gctcagggtg aatcacgacg tcgatatgct
     3481 cgtaatcggt gtcaaaaagc cagcgcgctt caatcagctc gagtcccttg ttcatgagcg
     3541 ttgcggaatc gattgttatc ttgccgccca tgctccagtt ggggtgcttg agtgcttgtt
     3601 cgacggttac atctcgaagt tggtccctgg accaagaacg gaatggtcca ccggaggccg
     3661 tcaagatgat gcgccgtacg tcttctgaac ggcctgcctg aagggactgc caaatggcac
     3721 tgtgctcgct gtctaccggc cgaatgacct gtccgagtcg cttcgcgagc ggcatgatca
     3781 attctccagc acatacgatt gcttccttgt tggccagagc gatggctttg ccggcctcga
     3841 gtgccgctat gacggcaggg atagcgtcgt ggcctgacgt agcaacaaca acaatatcga
     3901 catcagggtg agtcgccgct tcaatcaatt gctgcggtgt gggtcggttt ccatccggtg
     3961 attcgtgagc tgagacgacc atgtccgggc gaaatcgggc gacctgacgt gacagtagat
     4021 cctctcttag cccggcggcc atggttacca cccggaagcg atcttccatc gttgcgatga
     4081 cgtccagcgt ctgctcacca atagagcctg tggagcctag cagcgcgata ccgggcatga
     4141 cgaatcctcc atggtgatca acccaatgt
//
LOCUS       DASYWM010000046         6996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_602192, whole genome shotgun sequence.
ACCESSION   DASYWM010000046 DASYWM010000000
VERSION     DASYWM010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602192"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VGR29_01785"
     CDS             <1..461
                     /locus_tag="VGR29_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2072348.1"
                     /translation="NPVMLANIADTVDEISGGRVILGLGAGWVESEFRALGIPFGQRI
                     ARFEEAIQIITGLLREREVDFQGTWYAAQDSKLRPAGPRPQGPPIMVGTAVHGPRMVR
                     LAARYADIWNIGFRTRPGTLQLCPDTVRPCIVDLQGRLCTATSLCGRSTW"
     gene            complement(529..1077)
                     /locus_tag="VGR29_01790"
     CDS             complement(529..1077)
                     /locus_tag="VGR29_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072349.1"
                     /translation="MLTPYAVTSSRSTKHRYRSHHFGYAVTWTEPWVLDDPEETGSSI
                     ADPGTEAVVVRFTPEQAMARIIGVDRLDVLNPAAIARYWTSPAYLSKYMAAGTMVLLA
                     QQVGNLVAVVMVSPGTTPDNPIVTVKEVDDLGPRIRIQVTFTVELATSQEAFRSLQQC
                     VLIEGRPPIRAIDADLLLAVLS"
     gene            1279..4011
                     /locus_tag="VGR29_01795"
     CDS             1279..4011
                     /locus_tag="VGR29_01795"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2072350.1"
                     /translation="MTGTRGAPFGTVLRALRNRAGLTQEELAERAGLSVRGISDLERG
                     VNRRPHGETVRLLADALGLESNEQANLMAAGRPVPSQTLPPSRLPLPPTSLIGREREI
                     SEVSALLTRPDVHLLTLTGPGGVGKTRLALAAASEVQEAFPDGVWLVDLAPLTNPDLV
                     PGSIGEVLGVRETGGTPLLELLQEYLRNKNLMLVLDNLEQLLPDAAPGIGDLIAAAPG
                     LKALVTSRGPLHLRAEREIAVPPLGLPRRKPLPTLEQLGQYEAVRLFISRAQAVKADF
                     VVTTANAPAVTEICHRLDGLPLAIELAAVRIRLFPPEAMLTRLRNRLPLLTGGARDLP
                     ARQQTLRGAIAWSYDLLSPGEQALFRRLSVFAGGFTLEAADAVAAGDQLGLDMVESLE
                     RLTEQSLVQQTEESGEPRFTMLETIREYGLEQLEGQGEADEAHQRHGAYFLSLAEEVE
                     IALKGVTGQGAWLDRLETEHDNMRSALSWSLEHHPGMAMALAGALGRFWEIRGHLSEG
                     HDWLAQALANSDDLPSAVRAKALFAAGVLVESQAQSTADLASAHTFVEESLALQQRIG
                     DRPGIARCFNMLGVLALRQGEYRRAEAFWEETLTRHHDPNNKRAGSLVLANLGIVSTI
                     RGDMRKATAQFEEALALQREIGDQVGIAATLQNLGEVSAWRGDYDRAVTLFEESLVLN
                     RELGNKAGVVDILVSLARLRTSLGDVAEAETTLAEARDLAQELGNPSITIGLLMSEGN
                     WALESGDHERAVACLEEALALSRAVGDRRSEGSALAGLAEVARRRGIALEATDRFRQA
                     LTLMSELENKDGVGYVLNALALLSLETGRLAPAARLLGAAEKLRESLGIVPAPIERPE
                     LERANAALRADLGEDIYAGAHAAGAALSLEKAIAEALAVDEEMTGDTDGTEAAG"
     gene            complement(5149..5841)
                     /locus_tag="VGR29_01800"
     CDS             complement(5149..5841)
                     /locus_tag="VGR29_01800"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEV2072351.1"
                     /translation="MYFQHSSDIWDDFLELVPGVLSAGGIHPDVDVESRIASWQQVAE
                     ERLADATESTLPEIQAWRRTFAKMGLKPTQYRCASESLLRRFRKEGSLPRLHPLIDLC
                     NALSIAFAIPIAVLDTSKVTDWIEVRHASGEESYLTFAGETEHPAPHEVIFADGAGRA
                     HARRWTNRQSGYSAVQNSTASVLIVAEAMHESAADDIQRLVATIGEEVSSIWSATPAG
                     TVLTRSDPRFVF"
     gene            complement(5880..>6996)
                     /locus_tag="VGR29_01805"
     CDS             complement(5880..>6996)
                     /locus_tag="VGR29_01805"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEV2072352.1"
                     /translation="GHAQEAAIELLRFETVPYPKAVRDELFALYEDQRNAVARLCAMN
                     VVVGQCFAEAAADQLVLAGIDGSEVEVIGSHGQTVWHQPEADSGNPLIVPSTMQMGEA
                     SVIAARIGAPVMADFRVADMAVGGQGAPLAPYLDWAVFSDATKSRCVQNIGGIGNVTW
                     LPAGGAVDEVIAFDTGPGNMLIDALVHRLTDGAQAYDADGAIGAQGAVDADLLARLMD
                     DPYLDQAPPKTTGREYYGSGMVKELVTRTGLSAGAWSGDDPALRQRAADLVATATAFT
                     ASSIADAYHRWLPAMPDEVYINGGGVRNPLLMRMLQDALEIVPVRSTDELGVNADAKE
                     AMAFALMAHDALAGLPTNIPGATGANRAVSLGKLTRL"
BASE COUNT         1291 a         2191 c         2202 g         1312 t
ORIGIN      
        1 gcaatccggt catgctggcc aacatcgccg acaccgtgga tgagatcagt ggtggccggg
       61 tgatcctggg gctcggcgcg ggctgggtcg agtcggaatt tcgcgccctg ggcatcccct
      121 tcgggcagcg cattgctcgc ttcgaggagg cgatccagat catcaccggc ctgctgcgtg
      181 agcgggaggt cgacttccag ggcacctggt atgccgccca ggattccaaa cttcgccctg
      241 ccgggcctcg cccgcagggg ccacccatca tggtggggac cgcggtccat ggcccccgca
      301 tggtcaggct cgcggcccgg tacgcagaca tctggaacat cggtttccga acccgaccgg
      361 ggacgctgca gctatgccct gataccgtga ggccgtgtat cgtcgatctc caggggcgtt
      421 tgtgtacagc aacctcccta tgcggccgtt ccacttggta gacacggatg cattcgcctt
      481 gccgcggttc tccctgtctg gaagcggcga ggctacggtg cgactgtcct aggacagaac
      541 cgcgagcaag agatcggcgt cgattgcacg tatcggcgga cgaccctcga tgaggacaca
      601 ttgctgcaga ctccggaagg cctcctggga ggtcgccaac tcaacggtga aggtgacctg
      661 gatccggatt ctgggtccca agtcgtcgac ctccttcacc gtgacgatgg gattgtcagg
      721 tgtagtgccg ggactcacca tcaccacggc gacgaggttt ccgacctgct gggccagcag
      781 caccatggtg ccggccgcca tgtacttgga gagatacgcc ggggaagtcc agtaccgggc
      841 aatcgcggca gggttgagca cgtcgagtcg gtcaacgccg atgatccgtg ccatggcttg
      901 ttccggggtg aaccgaacga cgacggcctc ggttccagga tccgcaatgg aggacccggt
      961 ctcctcaggg tcgtccagga cccatggctc ggtccaggtg acggcatagc cgaaatggtg
     1021 gctgcggtag cggtgtttcg tcgaacggga ggatgtgacg gcatagggag ttagcatggt
     1081 ggaatgcctc gctctggtgg ccttgggacg gtgtactggc gcttcggcct ggtccttggt
     1141 ggtcctcgct ggacctgttt ccatgatgga gccgggagcg gcgctctccc acgcaggatt
     1201 cccacgtagt gacccacgtg attcaattgg gcaattcatg tgggctgcgg cacacgctgc
     1261 agaggcgagg agatcgggat gacagggacg aggggtgcgc cgtttggcac ggtgctgcgc
     1321 gccttgcgca accgggccgg gctgacccag gaagaactgg cggagcgagc cgggctctcc
     1381 gtgcgcggaa tcagcgatct ggaacggggg gtcaatcgtc ggccccatgg tgaaaccgtc
     1441 cggctcctgg ccgatgcctt ggggctcgag agtaacgagc aggcgaacct gatggcagcg
     1501 ggccgtcctg tgccgtcgca gacgctgcct ccctcccggc tccccttgcc gccaacctcc
     1561 ttgatcggac gggaacggga gatcagcgag gtgtcggcgc tcctgacccg tcctgatgtt
     1621 catctcctca cgctcaccgg ccccggtggc gtcggcaaga cccgcctcgc gctggccgcg
     1681 gccagcgagg tgcaggaggc atttccggat ggcgtctggc ttgtggatct cgcgcctttg
     1741 accaatccgg acctggtgcc cggttcgatt ggcgaggtgc tgggggtacg cgagacaggc
     1801 ggaacaccat tgctggagtt gctgcaggag tacctacgga acaagaatct catgctggtc
     1861 ctggacaacc tggaacaatt gctaccagat gcagcccccg gcattggaga tctcatcgcc
     1921 gcagctcccg ggctgaaggc cctggtcacc agccggggac ccttgcacct gcgtgctgag
     1981 cgggagatcg cggtgccccc gctgggcctg ccccgccgca agccacttcc cacactcgag
     2041 caactggggc aatacgaggc ggtccggctc ttcattagcc gcgcccaagc ggtcaaggcc
     2101 gacttcgtgg tgaccactgc aaacgcaccg gctgtgaccg aaatctgtca ccgcctggat
     2161 gggttgcccc tggcgatcga gttggcagcg gtccggatcc gcctcttccc tccggaggcc
     2221 atgctcaccc gtctccgtaa ccggttgccg ctactgacgg ggggcgcccg ggacctgcca
     2281 gcccggcagc agacgctgcg gggtgcgatt gcctggagct acgatctgct gtccccgggt
     2341 gagcaagcgc ttttccgccg gctcagtgtc tttgccgggg gcttcaccct tgaggcggca
     2401 gacgccgtgg ccgcgggtga ccaactgggg ctcgacatgg tggagagctt ggagcgattg
     2461 accgaacaga gcctcgtgca gcagaccgag gagagcgggg aaccccgctt cacgatgctg
     2521 gagacgatcc gcgagtatgg gctggagcaa ctggaggggc agggggaagc ggacgaagcg
     2581 caccagcggc atggtgccta cttcctctcc ctggcggagg aggtggagat cgcgctcaag
     2641 ggtgtgaccg ggcagggagc ctggctggat cgcttggaaa ccgagcacga caacatgcgc
     2701 tcggcattga gctggtccct agaacaccat ccggggatgg ccatggcact tgctggagca
     2761 ctcggccgat tctgggagat tcgaggccac ctgagtgagg ggcacgattg gctcgcccag
     2821 gcactggcca acagtgacga tctgccgtcc gccgtacggg ccaaggccct ctttgcagct
     2881 ggcgtgttgg tggaatcgca ggcccagtcg acggcagatc ttgcgtcggc tcatactttc
     2941 gtggaagaga gtcttgcgct gcagcaacgt atcggggatc gcccaggcat cgcccgctgt
     3001 ttcaacatgc tcggggtcct cgcccttcgc caaggggaat accgccgtgc ggaggcgttc
     3061 tgggaggaaa cgctgacccg gcatcatgat cccaataaca agcgagcagg ctctcttgtt
     3121 ctagcaaacc tcggaatcgt atcgaccata cgcggggaca tgaggaaggc tacggcacag
     3181 tttgaagaag ctctcgcctt gcagcgagaa atcggggatc aggtaggaat tgctgccacg
     3241 ctgcaaaacc ttggggaggt atccgcttgg cgtggcgact acgatcgggc agtgactctc
     3301 tttgaggaat cccttgtcct gaatcgggaa ctgggcaaca aggcaggtgt cgtcgacatc
     3361 ctggtgagtc ttgcccgcct ccggaccagc ttgggggatg tcgcagaggc cgagacaacc
     3421 ctggcagaag ccagggatct cgcccaggaa ctagggaatc cctcaatcac aattgggttg
     3481 cttatgagcg agggcaactg ggcgctggag tctggcgatc acgagcgagc ggtggcatgt
     3541 ctggaagaag cgcttgccct gtcccgtgct gtgggggacc gaaggagtga gggaagcgct
     3601 cttgcaggac tggctgaagt tgcgcggcga cgagggattg ctctggaggc cactgaccga
     3661 ttccgccaag cactgacgct catgtcggaa ctcgagaaca aggacggtgt cggctacgtg
     3721 ctcaatgccc tggcattgct ctccctggaa actggacgac ttgcgccggc ggctcggctc
     3781 ctgggagccg ccgagaagct gcgggaatct ctcggcattg tgcccgcgcc tattgagcgt
     3841 cccgaactcg agcgggccaa tgcggctctg cgcgcagacc tcggcgagga catctacgcc
     3901 ggtgcccatg ctgctggcgc ggctctgtcg ctggaaaagg cgattgccga ggccttggcg
     3961 gtcgatgagg agatgaccgg cgataccgac ggtacagagg cggcgggata gaggggtaac
     4021 cgtcggtgca cgtacctcgg tgtgctgtca ggatgcgtct tggaaaccag tacgccatgc
     4081 agtccggcga atctctcgca tgcgcgggca tgacaacggc ccccgaggta ccgagagccg
     4141 cgttgcttgc gccttcaatg tggtgaggct gttaggtgct ctaagtcaat ccggctgact
     4201 caggaccaag agcagatggt ccccgaggcg tctacgaatc ggctggtatt ctgcacgcaa
     4261 ggatgtaagc ccattgaagt gagttggtgg tccatctctc gtgttcctgt cgctggatcc
     4321 acatttcatc ctgacgacac ggtgtttctc ggcaacctcc ccaagcgacg gaacgacgct
     4381 gaaggcatgc aacaccttgc gtttcacgta ttcgaattgg cgagcgcgtc tatctgtgcc
     4441 tcccggccgt cccacgggta ggtgtcttgc tactgacaaa ggggcttttc atagccactt
     4501 cccagtcaga acgtagaccg ccgaagccga aggacgatac ccgcatgtct ccgatgtcgc
     4561 aactatgacc gtaaacccga ccttcgcaat cccggtaacc aaatcgatct accccacgct
     4621 gcctgtcggg gtgagataac ggcgcagatc ctctttctgg acgcgtattg aagatagagc
     4681 gtctgcacca tctcgctggt catgtttgcc cgctctcggc aaggctgcca aataccatcg
     4741 agcgatctgg cagacctgaa agccgtatcg attcagggaa attggtccca tgtgggccaa
     4801 ggctgccaca ctgccatatt gagtgcatgg cagccttcgt cctcccaccg cgtcttacgt
     4861 tgccaggttc ttgcccgccc tgcaaacacc ttggccatct agagaaaccc ccgtcatgtc
     4921 aaggaaatca ggctgcagga acctttaggc tgccaccggc caggttatgt acttggccac
     4981 ctaagactac gttcaactgg ccgcgtttct agaccacaga tcgttttcca gccacacgcc
     5041 atcaatcgca ccggtcgcag cctgaccgat ttgtctgcct gtcaaccacg gaatcatcac
     5101 tgtttacagg cacggatcga gataggtccg tacggagggg gccagtaatc aaaaaacgaa
     5161 ccggggatca gatcgcgtga ggaccgtccc tgcaggcgtg gcggaccaga tgctgcttac
     5221 ctcttcccct atggtcgcaa caagccgctg tatatcatcg gcagcggatt cgtgcatcgc
     5281 ctcggcgacg atcagcacag atgcggtcga attctggacc gcggagtacc cgctctgcct
     5341 gttcgtccat cggcgtgcat gtgcccgccc ggcgccgtca gcaaagatca cctcatgcgg
     5401 tgccgggtgc tccgtctccc cggcgaacgt caggtacgat tcctcgccgg atgcgtgtcg
     5461 cacctcgatc caatccgtca cctttgatgt gtctagcacc gctatcggaa tcgcgaaggc
     5521 gattgagagc gcattgcaca ggtcgatcag cgggtgaagc cgtggcagcg acccctcttt
     5581 ccggaaccgg cgcaacaggg actccgaggc gcaacgatac tgggtcggct tcagtcccat
     5641 cttcgcgaac gtgcgtcgcc aggcctggat ttcgggaagc gtggattcgg tggcgtcggc
     5701 taaccgctcc tccgcaacct gctgccacga cgcaatccgg ctctcgacat caacatcggg
     5761 atggattccc ccggcggaaa gcacgccggg aaccagctcc agaaagtcgt cccagatgtc
     5821 gctcgaatgc tggaaataca tgccgcctca ccctcctgta gggagcaaac gatcgaggct
     5881 tacagccgtg tcagtttccc aagggacact gcgcggtttg ccccggtcgc gccggggatg
     5941 tttgtcggca acccggcgag tgcgtcatgc gccatcaggg caaaggccat cgcctccttc
     6001 gcgtccgcgt tcacgcccaa ctcgtccgtc gaacgaacgg gaacgatctc cagtgcatcc
     6061 tggagcatcc gcatcagcaa tggattccgc accccaccac cgttgatata gacctcgtcc
     6121 ggcatcgctg gaagccaccg gtggtaggcg tcggcaattg agctcgcggt gaacgcggtc
     6181 gccgtggcca ccaggtcggc cgcccgctga cgcaacgccg gatcatcgcc ggaccaggct
     6241 ccggcggaga gaccggtcct cgtcactagc tccttgacca tcccgctgcc gtaatactcc
     6301 cgcccggtcg tcttcggcgg tgcctggtcg agatagggat cgtccatgag acgcgccagc
     6361 aggtcagcgt cgactgcccc ctgcgccccg atcgccccat cggcgtcata cgcctgcgcg
     6421 ccatccgtga gcctgtgaac caacgcatcg atcagcatgt ttcccgggcc ggtatcgaag
     6481 gcgatcacct cgtccaccgc tcccccagcc ggaagccagg tgacattccc gatcccgccg
     6541 atgttctgca cgcaccggct ctttgtcgca tcggagaaga ctgcccagtc gagatagggc
     6601 gccagcggcg cgccctgtcc gccaacagcc atgtcggcga cccggaaatc cgccatcacc
     6661 ggcgcgccaa tcctcgccgc aatgacggac gcctcgccca tctgcattgt cgacggcacg
     6721 atcagcgggt tcccggaatc cgcctcgggc tgatgccaga ccgtctgccc gtgcgacccg
     6781 atgacctcaa cctcgctccc gtcgatccca gcgagcacga gttgatcggc tgccgcttcc
     6841 gcaaagcatt gccccaccac gacgttcatt gcgcagagac gggcgaccgc gttccgctga
     6901 tcctcgtaca gcgcgaagag ttcgtcccgc actgctttcg gatagggaac cgtttcgaac
     6961 ctgagcaatt cgattgccgc ttcctgcgcg tgacca
//
LOCUS       DASYWM010000047         4045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_508165, whole genome shotgun sequence.
ACCESSION   DASYWM010000047 DASYWM010000000
VERSION     DASYWM010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508165"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VGR29_01810"
     CDS             <1..1015
                     /locus_tag="VGR29_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072353.1"
                     /translation="VSPLQGEKPMRRLFFGLLAALLLMPVSILSVLAAQDATPASGLA
                     DADVPQLDVTLTATGYEGIPDQVEAGRYLVSLTLEEGASEFGGGVSFVQPPADISAED
                     FLVAYMTPSDESGVGEAAATPIEGGVATPAEGEGEMGGLPSFLFEATYAGGTYSFAGG
                     VTEVVLDLTPGEWIAWTDDFEAPQEPVFFEVTGEMPAELTEPESSATITMGEYVIEVT
                     EGELTSGSQVIRIDNIGAQPHFLAWFGLPEGTTEEQIQTVLDEEMQAEMTGTPAAFSD
                     LNPDEDFMPVVFTATQSSNTSIWITVDLEAGTHGLACFFPDLSDGMPHAYHGMYTVIE
                     VAE"
     gene            complement(1966..2700)
                     /locus_tag="VGR29_01815"
     CDS             complement(1966..2700)
                     /locus_tag="VGR29_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2072354.1"
                     /translation="MMNRTIEQQTEQASPIVDETEIERRLTTFLRSRLGVWPPQESVQ
                     VVPWPVRDEPGWDGSSLPLVGIESPLGTVLSYSPTRLPEAADIDPIRLEDELGTADAY
                     MTLPQLFGRPDLHFGRGVFRYVDQPADLPEIGEWVPRDDPRLPPWLLPFNGDVLVAWD
                     ENGQYAAGVGLKKHNEYGHEISVGTDPAHRGQGLARMLVAQAAKQILRDGAIPIYLHG
                     DRNAASKRVAEQSGFPDRGWHIIELR"
     gene            complement(2863..3375)
                     /locus_tag="VGR29_01820"
     CDS             complement(2863..3375)
                     /locus_tag="VGR29_01820"
                     /inference="COORDINATES: protein motif:HMM:NF025686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4385 domain-containing protein"
                     /protein_id="HEV2072355.1"
                     /translation="MPKPRRQPPFDYDLPFDQIDFRERPDLYRIGKGEQGVLLVEPYK
                     SELLPHWRFRTPDIARASAAKLTECFNVYRAANDFVGMDMARKFLQMGFTRSRRYANH
                     KSGRKWSPGREEVLPDDIDPVKAECARIFYTAYVAARKDPEYKRLKQEHLGRYPGPTA
                     EGPEKGTPTS"
     gene            3576..>4045
                     /locus_tag="VGR29_01825"
     CDS             3576..>4045
                     /locus_tag="VGR29_01825"
                     /inference="COORDINATES: protein motif:HMM:NF015789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072356.1"
                     /translation="MRIGYPCSNRSLDVSAARTFRLASYTPERLIETVEANLLALRTI
                     LEWNVDHGILFFRISSTTVPFASHPIMDVDWQSICAPRLAELGELINAHDMRINMHPG
                     QYTLLNAPKPEVVQQSIAELRYHAELLDLLGLDHTHKIQIHTGGVYGDKVAASER"
BASE COUNT          725 a         1189 c         1346 g          785 t
ORIGIN      
        1 cgtttcgcca ttacaggggg agaaacctat gcgccgtctc tttttcggat tgcttgccgc
       61 cttgctgctg atgcccgtga gcatcctgtc tgtccttgct gcccaggatg cgactccagc
      121 cagcgggctc gctgatgcag acgtgcccca gctcgatgtc acgttgaccg caactggcta
      181 tgagggcatc ccggaccagg tcgaggccgg tcggtacctc gtctctctga cgctcgagga
      241 aggcgccagc gagtttggcg gtggcgtctc cttcgtgcag ccgccggccg atataagcgc
      301 tgaggacttc ctcgtcgcct acatgacgcc ctctgacgag agcggagtgg gcgaggccgc
      361 tgccacgccg atcgaaggcg gcgttgccac ccccgccgag ggtgaggggg agatgggtgg
      421 cttgccgtcc ttcctctttg aggccacgta tgccggcggc acgtattcct ttgccggtgg
      481 tgtgacggag gtcgtgcttg acctgacgcc gggcgagtgg atcgcctgga cggacgactt
      541 cgaagccccg caggagcctg tcttcttcga ggtgaccggc gagatgccgg cggagctgac
      601 cgagccggag tccagcgcca ccatcacgat gggtgagtac gtgatcgagg tgaccgaggg
      661 cgagctgacg agtggctccc aggtcatccg catcgacaat atcggtgccc agccccactt
      721 cctcgcctgg tttggcctgc cggagggcac gaccgaggaa cagatccaga ccgtgctcga
      781 tgaggagatg caggcagaga tgacgggcac cccggcggcc ttctccgacc tgaatcccga
      841 cgaggacttt atgcccgttg tcttcaccgc cacccagtca tcgaacacca gcatctggat
      901 caccgttgac ctggaggcgg ggacacacgg cctagcgtgc ttcttcccgg atctttcgga
      961 cgggatgccg catgcctacc acggcatgta caccgtcatc gaagtcgccg agtagcgata
     1021 cgcaatacgt cgatagcaaa gcgaggccag ccgggcgccc ggctgacctc tctttgcatg
     1081 gactccttcg cccgcgtgta gccttcagag gtggggacgg ggggacgtgc gaatcgctcc
     1141 caacatgcca tccttccggg catcgggggg accttatgtc cgctcctacc aggatgcgga
     1201 cgacctcggg agcttgggag gcgggcctct gccgtaggga tttgcaatgg ggttgatacg
     1261 caggcccagc caggggctcg ttggtgcacg gctgcggcgg caaagccatc aagaagcgga
     1321 ccgagcccat ggccgaggtg gccgcaccat gaagaagcgg agtgagccct ttcatccaag
     1381 gcctcacccc tgcgacatgc cgcagaaata tcgtccttag cggcctgcca ggaggacatt
     1441 gctgcattcg tgatcgcgaa tgggccgcag gcaccgcaag tcctccgcta cagacgacag
     1501 acgcgacaga cctgcaacct cgctgaacac agatccaagt ggcgcttatg gcatctgggg
     1561 atgtcgaggg gtgcgacatc caccaccgac gcgcttcgcg cggggatcct tccacacggg
     1621 gacgcgaacg acgtgcgcgg agctggctca ggatgacggg ggtgggccgt gtcgcgcaag
     1681 gctttgcgtt cccttgcttc cctaaggatg gcggagtggt ggggcatttc gctcggggca
     1741 ttgctgccat tgcctgcgct caggacgacg gggcgtttgg ggcggcgctt tgtgccctcc
     1801 tggcatcacc tcgggtccat cgctgtgctc tgagtgacgg gtgtggtggg acgtttcgcg
     1861 cggggcattg tgcccttttg ctacgaacag gcaagctctc tggaaacagc cctaacccca
     1921 agcgatgggt gcattgcggg caggtttccg cgaagggagc gtttgctacc gcagctcgat
     1981 gatgtgccag ccgcggtcgg ggaagccgga ttgttcggcg acacgcttgg aggcggcgtt
     2041 gcggtcgcca tggagataga tcgggatggc gccgtcccgc aggatctgct ttgcggcctg
     2101 ggcgacgagc atgcgggcga ggccctgccc acgatgggcg ggatcggtgc cgacggagat
     2161 ctcgtgcccg tactcgttgt gcttcttgag gccaaccccg gcggcatact ggccgttctc
     2221 atcccaggcg accagtacat caccgttgaa cggcagtaac caggggggca ggcgagggtc
     2281 gtcgcggggg acccactcgc cgatctcggg gagatccgcc ggctgatcga cgtagcggaa
     2341 gactcctcga ccgaagtgga ggtctggccg gccaaagagc tgcgggaggg tcatgtaggc
     2401 gtcggcggta ccgagctcat cctccaggcg gatgggatcg atgtccgcag cctccgggag
     2461 cctggtgggc gagtaggaga gcaccgtgcc gagcggagac tcaatcccca ccaaagggag
     2521 ggacgagccg tcccagccgg gctcatcacg tactggccag ggaacgacct gaacggattc
     2581 ctgcggcggc cagacaccga gcctgctgcg gagaaaggtg gtgagccggc gttcgatctc
     2641 cgtctcgtcg acaatggggc tcgcctgttc agtctgctgc tctattgtcc tgttcatcat
     2701 agtcatgctg cttcaaggtc tcagggttac gtcgtcagca agcaagaatc tcctgccatg
     2761 ccgttcgtac gcatggattg tacccagagg gtacggcgct gccttcagac agtcggttca
     2821 taagcgactg ccaggcaatg tgcgatggac gggaatcgct gttcaggatg tcggggtgcc
     2881 cttttcaggc ccttcagccg ttggccccgg gtaacggcca agatgttcct gcttcaggcg
     2941 tttgtattcg gggtccttcc gggcggcgac gtaggccgta tagaagatgc gggcgcattc
     3001 agccttgacg gggtcgatgt cgtcgggaag gacttcctcc cggcccggtg accatttgcg
     3061 accactcttg tggttggcat atcgacggga gcgggtgaag cccatctgca ggaacttgcg
     3121 ggccatatcc atgccgacaa aatcgttcgc ggccctgtac acattgaaac attctgtgag
     3181 tttcgcagca gaggcacggg caatatccgg ggtgcggaac ctccagtggg gaagcagctc
     3241 ggatttgtag ggttccacaa ggaggacgcc ctgctcgccc ttgccgatac gatagaggtc
     3301 agggcgctcg cggaagtcga tctgatcgaa ggggaggtca tagtcaaatg gtggctgacg
     3361 gcgtggcttg ggcatgatga tgctccccga tcaggacgcg ggaacgtcag attgtcggga
     3421 tgttcgttgc caacatgcgg catgggtcgt gctctcacgt tgtcatgtcg cgccgtgcgc
     3481 atgacatcct ggcgagccga gccggtatct tccggccgca gtgaagttcc ggatcaggat
     3541 accagtgcat gggtgccggg aaagatggag gcagtatgcg tatcggctat ccgtgctcga
     3601 atcgttcgct cgacgtctcg gccgcgagaa cctttcgcct ggcgtcgtac acgccggagc
     3661 gcctgataga gactgtggag gcgaacctcc ttgcactgcg aacgatcctg gaatggaatg
     3721 tcgaccatgg tatcctcttt ttccggatca gctcgacgac cgtgccgttt gcgtcccacc
     3781 ccatcatgga tgtcgactgg caaagcatct gtgcgcccag attggcggag ctcggcgagt
     3841 tgatcaatgc tcatgacatg cggatcaaca tgcatcccgg tcagtacacg ctgctcaatg
     3901 cgccgaagcc ggaggtggtg cagcagagca tcgccgagct gcgatatcac gccgagctgc
     3961 ttgacctgct tggtctcgat catacgcaca agatccagat ccatacaggc ggtgtctacg
     4021 gggacaaggt ggcggcaagc gagcg
//
LOCUS       DASYWM010000048        11691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_76806, whole genome shotgun sequence.
ACCESSION   DASYWM010000048 DASYWM010000000
VERSION     DASYWM010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76806"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..668)
                     /locus_tag="VGR29_01830"
     CDS             complement(174..668)
                     /locus_tag="VGR29_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072357.1"
                     /translation="MPTRQGDLALLDDPVAHRLLQSTLPARFAYNWDDGTPRVVPIGF
                     HWNGTELVLGTPPDAPKMKVLGDGAKVALTIDSDTMPYKVLLIRGTVRTDTVDGIAPE
                     YEAMTVRVMGEEQARAWLENMRPICPRMSRVFVTPEWVGILDFETRFPSAIERAVEQA
                     QSQA"
     gene            complement(853..2235)
                     /locus_tag="VGR29_01835"
     CDS             complement(853..2235)
                     /locus_tag="VGR29_01835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV2072358.1"
                     /translation="MKDYRGYSFWLETCGDDLTPRPPLDGSRDVDIAILGAGFTGLWT
                     AYYLLSKEPSLKIAIVEAEIAGFGASGRNGGWCFSGFPVSPLSLLQSYGYDTARLVSL
                     EMYKAVDEVGRVTREEGIDARYAKGGELEVARALYDVPKLQQTYDEFRAIGLEDHNQL
                     LDARETQERINVAGAVGSFWNKEGAAIQPARLARGLARAVERLGGTIYEGTRVTDFTP
                     APDASLVTDRGTVHAKSVVLAGEAYLSALPKLSRSIIPLTSHMVVTEPLSDDQWDQIR
                     WRERDVLGGFGVKGGYINHTSDGRIAFGAYRANYPYKSRITDDINLDEKVFAHAREAT
                     LDWFPMLQGVRFTHAWGGVFGVPRKRMPTMTYDPGTGIASAYGYSGEGVATANLSGRV
                     LADLITETDSSLTTLPMTQSGDSKWEPEPLRWAGVTFVRRGRLRMLQKVEREGKYPEK
                     PTLAQRLYNY"
     gene            complement(2275..3228)
                     /locus_tag="VGR29_01840"
     CDS             complement(2275..3228)
                     /locus_tag="VGR29_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072359.1"
                     /translation="MINTTQSASSRLTLDRVGPALAPDQGLRSKPQSMARVYIRRFLR
                     HRLAAAGLAVLVLITLMAVLAPLITQDPNALSASDRKAAPSAGHWFGTDEAGRDILAR
                     TIHGGRISLAVGIAATGVALLIGTLVGAVAGYFGGLVDAVLMRVTDAFLCFPQLFVLI
                     VFGTLMRTTELRVLQGNMTPIAIIIGLLSWMGLARIVRGTFLSLREQDYVEAARSVGA
                     GNVRIMVRHILPGAVGPIVVQATLLIAVTIITESGLSYLGFGVQPPTSSWGNLLEGAK
                     PFLQTLPVYAIAPGMMIFLTVMSINFVGDGLRDALDPHSRH"
     gene            complement(3245..4234)
                     /locus_tag="VGR29_01845"
     CDS             complement(3245..4234)
                     /locus_tag="VGR29_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072360.1"
                     /translation="MGAYIVRRILWAIPLLLFISIVVYGLLELSPGGPTSMYMRRGSG
                     ISAEDLARVEESLGLNDPFYVQYARWLGRVVQGDFGMATTSSRPVIDEIVDRLPNTLY
                     LMGAAWGVSLLIAIPIGILSAVRQYSKFDHFVTALTFVGQSIPSFFLGLLLLLIFYMQ
                     LDNPFTGGPLLPSGGVKTIGENLTGFAMIKDRVLHLILPVTMLSATWVAWYSRFLRAS
                     MLETIHLDYVRTGRAKGLRERIVILRHAFRNAAIPLVTLMALDLPLLFAGALFAEVIF
                     SWPGMGRLFYQAAERRDYGLLMAIIMITSLLIVIGNILADVAYRLLDPRIKLE"
     gene            complement(4328..6082)
                     /locus_tag="VGR29_01850"
     CDS             complement(4328..6082)
                     /locus_tag="VGR29_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2072361.1"
                     /translation="MATKRLNRRRFVVTSAGAGLVAGSVMRPQFGVMAQEATPETGQT
                     YYEDTQGPPQPGGTLRYLLYEDPNSLNPIMGATTIALQVVAAILEGLAESAPDGTFVP
                     ILAAELPTPENGGISEDLLTVTWKLKEGVVWSDGTPFTSEDVRFTWEAARDAANGSTL
                     ATDYALIIGVETPDDLTVVVTYSDFNAGYVDQFPWILPKHATGEIADMRNWAFNRAPI
                     GTGPFKLDEWSSGEFLQLSANENYREENKPYLDELLFLVIPDESVRTAMMIEESAEIM
                     LWSGQEADEQIEASGVGAPRTAPGVWVVEMRFNLSRPFDGDPGVDPPHPILGDIRTRR
                     AITHGVNRDRINNELITGTVYELDSPLQVGWMDCQVEPFVFDPEAAMALLEEVGWRDE
                     DGDGIREAHGVETVEDGTRLSLMMNGYTGFDTLDLIELAVQEDLMNIGMEIQIENQEF
                     AVIFGTWEDGSPRMLGDYDILIYDAGLFAEPGADIARNYGPDQVPGPDNQGGENFHRW
                     VREDVGEWIAAANSSPDLEVRRENFCKVADALREDIVSFPILQFSEGSAYSNKLHNFA
                     VSTWEWSTWDVENWWLEQ"
     gene            complement(6442..7242)
                     /locus_tag="VGR29_01855"
     CDS             complement(6442..7242)
                     /locus_tag="VGR29_01855"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2072362.1"
                     /translation="MTRIAILGALASASIRSEFQYRANAITSMIGGMLYQFTGFVTVW
                     IVVARFNDIGGWSLGEITFLYGMRLTSHGIFYACFSQMFEADRVLLTGEYDRYLLRPM
                     PPLLQLFTRKLRINAFGDLIGGAMLLTAASFGISVEWSPLTVLYLLAAVTGGALVEGA
                     VQISLGSLSFRFLQTMSVRTTVNEVFNLYGNYPFSIFPKMLEYLLTFVLPVAFVAFLP
                     STLLLDREDSLHVSPALAAIAPLVGVLLFLVALRIWRWQSRNYQSSGH"
     gene            complement(7244..8050)
                     /locus_tag="VGR29_01860"
     CDS             complement(7244..8050)
                     /locus_tag="VGR29_01860"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2072363.1"
                     /translation="MTIRANLPAWIEIGRANIRWQYMYRFNVLMGILLTGVTIYLLTI
                     VWRAAYGGQTEVGGIGIQQTLVYLTIANLQLYFLRPQVSGTIQERIREGQIGFDLSRP
                     VGYPSQLIARAAGDMVGLLPMVVVSVPVALIVGELEPPASFNAGLAYVVSFLLAWVIA
                     VQLNMVIGLVSFWTLEMTGFEMMYRLIGNFATGALVPLWFMPDVLRAIVQVLPFQSIA
                     FVPVSIYVGAPATGAVWSALAIQVFWAVALVGIIRWVWERAFLRTVIQGG"
     gene            complement(8047..8679)
                     /locus_tag="VGR29_01865"
     CDS             complement(8047..8679)
                     /locus_tag="VGR29_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEV2072364.1"
                     /translation="MTYLSSYRMGNNSECLRSQGQNSRAGIILNALDVFGDTRARNWD
                     REARDLERLGYSSEEVDLRQYFRDNEGLRHRLEALDLVWVVGGSAFALARAMVSSGFA
                     TALEPGMQRRLIYAGYSAGACVAGPDLRGINFMDDPDDIPAGYDPAVPPLTLGLVPFR
                     IVPHWKSDHPDAANAQRTVEYLERSSLPYKALRDGEALVIDEHKPGEVRS"
     gene            complement(8676..9689)
                     /locus_tag="VGR29_01870"
     CDS             complement(8676..9689)
                     /locus_tag="VGR29_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2072365.1"
                     /translation="MPIIQVDHLVKEFRRPRRIGGRLGGLRTLFTQQYDTTRAVDDVS
                     FTIDEGELIGYLGSNGAGKSTTIKMLSGILVPTGGEVRVGGLVPWEKREQNALQIGVV
                     FGQRTQLWWDLPLEDSLRLIGKLYRVDAARYARSHSHLVDLLGLEPFLRTPVRQLSLG
                     QRMRGDLAAAMLYEPRILYLDEPTVGLDVVAKERMRQFVEETNRERGTTIMLTTHDLA
                     DVERLCKRIIFIDKGRVLYDGSVAELKRRYAPWRVLEVTLAGETEDEIPPLGLPGAEI
                     VEQGAARLSIRFDPALIAVADLITAVIARHPVSDISIVEPDLEGVVREIVEAGEVRST
                     SPT"
     gene            complement(9775..10143)
                     /locus_tag="VGR29_01875"
     CDS             complement(9775..10143)
                     /locus_tag="VGR29_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV2072366.1"
                     /translation="MGTDNESRPVVRAWIDAVNAADMGKVDRLVADTVSIVGPRGSTT
                     GREAVSGWVHHTQIRMTVADTRIEGDRVIARVSATWQVDGGQPGERTPPATIFMAFTV
                     HEGRITTIQRLHSPEDTVQD"
     gene            10420..11040
                     /locus_tag="VGR29_01880"
     CDS             10420..11040
                     /locus_tag="VGR29_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072367.1"
                     /translation="MTAARANRLVPLGPIVRLQVQVEKIKTGEGIHQRYTPDQNLRTV
                     EALLLDSGGVYGRSLGGETLPDVHHRDHPRSRFRGANGISLLTTGHYAKMRERFGGHL
                     TDGIAGESLLVQCDGVMTLDALANGIVIGEGEDAVSVETWEMAHPCTPFSRFALQFPD
                     DQKPDRRITEALQFLDDGTRGFNGLYHGQSPNPVEIRLGDMVYLRA"
     gene            complement(11267..>11691)
                     /locus_tag="VGR29_01885"
     CDS             complement(11267..>11691)
                     /locus_tag="VGR29_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEV2072368.1"
                     /translation="VGNYSKAVYDVLAPHQPDLLIMAGFLRRLLVFPGWEGRILNIHP
                     ALLPDAAAYAAGKGRYGDRVHEAVLARGDRVSGATVHVVTDVYDDGPPLLRSEVPVEP
                     GDTAQSLAARVFAAECELYPEAIRQYMAAHPELKRSGS"
BASE COUNT         2509 a         3574 c         3411 g         2197 t
ORIGIN      
        1 cataaggaac cgacaccaga acaaccagga gctggtacgt gggtgaatat cgagggaaac
       61 agccagctga agcgtaggtg gagcccaagc acgaacaggt ttcgtcaccc gacgaatgtg
      121 ttggtccggc gggatggtgc atcgcctgtg gtaatgaatg ctctacggct cgcctacgcc
      181 tgcgactgcg cttgttccac ggctcgttca atggcgcttg ggaatcgcgt ttcgaagtcg
      241 aggataccga cccattccgg ggttacgaag acgcgggaca tccgcgggca gatcggacgc
      301 atgttctcca gccaggcccg cgcctgctcc tcccccatga cccgtacggt catggcctca
      361 tactcgggcg cgatcccgtc gacagtatca gtccggaccg tgccacgaat cagtaacacc
      421 ttgtaaggca tggtgtcact gtcgatcgta agcgccactt ttgccccgtc gcccaagacc
      481 ttcatcttgg gcgcgtccgg tggcgtgccc aacacaagct cggtgccatt ccagtgaaat
      541 ccaataggca caacgcgtgg tgttccatcg tcccaattgt aggcaaaccg tgccgggagg
      601 gttgattgaa gcaggcggtg cgctaccggg tcgtcgagta aggcaaggtc cccttgtcgc
      661 gtcggcatgg ctgtctccct gatggcgaca tcgactatgt agataatgac cctgagtccc
      721 cagcttgcac cccgatgccg ggaatgtcaa ctgtgcaaaa gtgagcaacc gtgcctcgat
      781 actcgttcat gtagctccgg ctccgtcatg acgagacctt gcgggatcag taaaatgact
      841 accttggcct gtctagtagt tgtagaggcg ctgggccagt gtcggcttct ccgggtactt
      901 gccctcgcgc tccaccttct ggagcattcg cagccgtcca cgtcgaacga aggtcactcc
      961 ggcccatcgc aagggttcgg gctcccactt tgaatcgccg ctttgggtca tgggaagggt
     1021 ggtgagcgac gaatcggttt cggtgatgag gtcggcgagc accctgccag agagattcgc
     1081 ggtcgcgacc ccttctccgg aatacccata ggctgaggca atgccagttc ccgggtcgta
     1141 ggtcatcgtc ggcatgcgct tgcgagggac accaaagaca ccaccccagg cgtgggtgaa
     1201 ccgaacgccc tgcaacatcg ggaaccagtc gagcgttgcc tctctcgcgt gggcaaagac
     1261 tttttcgtcc agattgatgt cgtcggtgat cctggatttg taggggtaat tggccctgta
     1321 cgcgccaaac gcaatccgtc catcggaggt gtgattgatg tacccgccct tgacgccgaa
     1381 tccaccaagg acatcgcgct cgcgccaccg aatctggtcc cattggtcgt cgctcagggg
     1441 ttcagtgaca accatgtgcg aggtgagcgg gatgatcgat cgactgagct tcgggagtgc
     1501 cgagagatag gcctcgccgg ccagaacaac cgatttggca tgcacggtac cgcgatcggt
     1561 gacaagcgaa gcgtcagggg cgggagtgaa atcggttact cgcgtccctt catagatcgt
     1621 cccgcccaag cgctccaccg cccgggcaag cccacgggcc agccgggccg gttgaatcgc
     1681 ggcgccttcc ttgttccaga aacttccgac cgcaccggcg acgttgatcc gctcctgggt
     1741 ttcccgcgcg tccagcagct ggttatgatc ttcgagacca atcgcgcgga actcgtcgta
     1801 ggtctgctgg agcttcggca cgtcatatag cgcccgggcc acttcgagct cgcccccctt
     1861 ggcatagcga gcatcgatgc cctcttccct cgtcacacgg cctacttcat cgacagcctt
     1921 gtacatctcg agcgagacca gacgcgctgt atcgtagccg taggattgga gcagcgagag
     1981 cggggaaaca ggaaacccag agaaacacca gccgccatta cgccctgagg ctccgaaccc
     2041 ggcgatctcc gcttcgacga tcgctatctt gagcgacggt tccttcgata gcagatagta
     2101 ggccgtccag agcccggtaa aaccggcgcc gagaattgca atatcgacgt ccctcgaccc
     2161 gtcaagcggt ggtcggggtg tgagatcgtc accacaggtt tcgagccaga agctatagcc
     2221 tctatagtct ttcatcgatt gtccttctat tgattccggt gcgttgacat tgttctagtg
     2281 gcgactatgg ggatccagcg catcgcgcaa gccgtcaccg acgaagttga tactcattac
     2341 cgtgaggaaa atcatcatgc ccggtgcaat agcgtagacg ggcaatgtct ggagaaatgg
     2401 cttggcaccc tcaaggaggt tgccccaact cgaggtcgga ggctgtacgc cgaaaccgag
     2461 atagctgaga ccggattccg taataatcgt cacggcgatc agcaaggttg cctgcacgac
     2521 gatcgggcca acggcgcccg gcaagatgtg acggaccatg atgcggacat tgccggcgcc
     2581 gaccgacctg gcggcttcga cgtagtcctg ctccctcagc gacagaaagg tgccgcgaac
     2641 gatccgggcc agtcccatcc atgagagaag cccaataatg atcgcaatag gggtcatgtt
     2701 cccctgcaat acacgcagct ccgtggtgcg catgagcgtg ccaaaaacga tcaggacgaa
     2761 gagctgggga aaacagagaa aagcatcggt cacgcgcata agcacggcat cgaccaaccc
     2821 accgaagtac cccgcgacgg cgccaaccaa tgtgccgata aggagcgcca caccggtcgc
     2881 cgcgatgccg actgcaagtg agatgcgtcc accatggatc gtacgtgcga ggatatcccg
     2941 tcctgcttca tccgtaccga accagtgccc ggccgaggga gccgccttgc ggtcgcttgc
     3001 ggagagcgcg ttcggatcct gggtaatcag gggtgccaga accgccatca gcgtgataag
     3061 gaccaggacg gccaaccctg cggctgcgag gcggtgacgc aagaaccgcc ggatatagac
     3121 ccgcgccatc gattgcggtt tcgatcgaag tccctgatcg ggggccaagg caggtcccac
     3181 ccggtcaagt gtcagcctgg acgaggcaga ctgcgtggtg ttgatcatga atgcttccct
     3241 cgtcctattc caacttgatc ctcgggtcga ggagccgata cgcgacgtct gcgaggatat
     3301 tcccgataac aatcagcaat gacgtgatca tgatgatcgc catgaggagg ccataatctc
     3361 gacgctcggc cgcctggtag aagagcctgc ccatgccggg ccaggagaag ataacttcag
     3421 caaagagggc gccggcaaac aggagcggaa ggtccagcgc cattagcgtg acaagcggaa
     3481 tggcggcatt gcgaaatgcg tggcgcagga taacgatgcg ttccctaagc cccttggcgc
     3541 ggccagtgcg cacataatcc agatggatgg tttccaacat cgatgcccgc aggaagcgcg
     3601 agtaccacgc aacccaggtt gctgagagca tcgtgacagg caggatgaga tgcagaaccc
     3661 gatccttgat catggcaaag ccagtgaggt tttcgccgat cgttttcacg ccacctgagg
     3721 gtaagagcgg cccgccagtg aaaggattgt cgagctgcat atagaagatg agcagcaaga
     3781 gaagtcccaa gaaaaacgac ggaatgctct gcccgacgaa ggtgagcgct gtcacaaagt
     3841 gatcgaactt cgaatattgc ctgacagccg agagaatgcc gatggggatg gcaataagta
     3901 gactgacgcc ccatgcagca cccatgaggt acagcgtgtt tggcagtcga tccacgatct
     3961 catcgatcac cggacgcgat gaggttgttg ccatgccgaa atcaccctgg accaccctgc
     4021 ccagccagcg cgcgtactgc acgtagaacg ggtcattcag gccaagtgat tcttccacgc
     4081 gcgcgagatc ttcagcgctg attccgctgc cacgacgcat gtacatcgag gtagggccgc
     4141 cgggcgaaag ttccagtagt ccatagacga caatgctgat gaacaggagc agtggaatgg
     4201 cccacaggat ccgcctaacg atataagcgc ccatcgttat tctttcccgc caagcgccga
     4261 atggagtgat cccggcctct cgccgggatc actcctctgc gctcgtgcca ttggacgtgg
     4321 ggtgcgccta ctgctcaagc caccagttct ccacatccca agtcgaccat tcccaggtgc
     4381 tgaccgcgaa gttgtgaagt ttgttggaat acgcgctgcc ctccgagaac tgcaggatgg
     4441 gaaagctgac gatgtcttca cgaagcgcat ctgcgacctt gcagaaattc tctcgccgga
     4501 cttcaagatc aggtgaggaa ttagccgcgg cgatccactc gccaacatcc tcccgcaccc
     4561 agcggtggaa gttctcacct ccctggttat cgggaccagg cacctgatca ggaccataat
     4621 tcctggcaat atccgcccct ggttcggcga agagaccggc atcgtagatc aggatgtcgt
     4681 agtcacccag catccgcggc gatccatcct cccaagtgcc aaagatgacc gcgaactcct
     4741 ggttttcgat ctggatctcc atgccgatat tcataagatc ttcctgcacc gccagctcga
     4801 tgagatcgag cgtatcgaag ccggtgtagc cgttcatcat cagggagaga cgcgttccgt
     4861 cctcgacggt ctcaaccccg tgcgcctcac gaatgccatc accgtcttca tcgcgccacc
     4921 cgacttcctc cagcagcgcc atggccgcct ccggatcgaa gacgaatggc tcgacctggc
     4981 agtccatcca gccgacctgc aggggcgaat cgagttcata gacggttccc gtgattagct
     5041 cgttgttgat gcggtcgcga ttgacgccgt gcgtaatcgc ccggcgcgta cggatatcac
     5101 caaggatcgg gtgcggcgga tcaactcccg ggtctccgtc gaatggcctc gagaggttga
     5161 atcgcatctc aactacccag acaccgggag cagttctggg agccccaaca cccgaggctt
     5221 cgatctgctc gtctgcctcc tgtccggacc agagcatgat ctcggcgctt tcctcgatca
     5281 tcatcgcggt acggacggac tcatctggaa tgaccaggaa gagcaattca tcgagatatg
     5341 gtttgttttc ttcgcggtag ttctcattgg cggaaagctg caggaattct ccagacgacc
     5401 actcatcgag cttgaacggt cctgttccga tgggagcccg gttgaacgcc caattgcgca
     5461 tatcggcgat ctcaccggtc gcatgcttgg gcagaatcca ggggaactga tcaacatacc
     5521 ccgcgttgaa gtcggaatag gttacgacga ccgtcagatc atccggggtt tcaactccga
     5581 taatgagtgc gtagtcggtg gccagggtcg aaccgttggc agcatcccgg gccgcttccc
     5641 aggtgaagcg gacatcctca ctagtgaacg gcgtaccatc tgaccagacg acgccttcct
     5701 tgagcttcca ggtgacagtg agcaagtctt ccgagatacc gccgttttcg ggcgttggca
     5761 gctcagccgc gaggatggga acgaatgtgc catccggcgc actctcggca agaccttcca
     5821 ggatggcggc aacaacctgg agtgcgatcg tggtcgctcc cattatcggg ttgagcgagt
     5881 tcggatcttc gtaaaggagg tagcgaaggg tgccgccggg ttgcggaggt ccctgtgtgt
     5941 cttcgtagta ggtctgccca gtctcaggtg tggcttcctg ggccatgaca ccgaattgcg
     6001 gcctcatgac gcttcccgca acgagccctg ctcctgcaga agttacgaca aagcgtcgtc
     6061 gattcagcct cttcgttgcc atccgggctt ctccttccgc gcattgcgca ataccgcttc
     6121 aacaccgacg gttgccgcgg tgtgggataa ggacgtccat gcgccctgtc tttgggagtt
     6181 gtcacattct atgtgcgata tcgtttacgc gcaagcactc gtgcatctcc ctgcaccgat
     6241 atttgtgtaa aactcgagtt tcggtcacag atacatatgc agcagtgaga acgcccggtt
     6301 cacttatgta ccgggcagtt gcatgaactg gaaggcaaat tttgaggccc tattagcggt
     6361 caattgacgg gtaatccatg gtcctgaaac gctggtggtg gcgacatcca gcgcattaat
     6421 ctggacgaag atgagcgagt tctagtgacc agagctctgg tagttccggc tctgccatcg
     6481 ccagatccgc aatgcgacga ggaagagcag aactccaaca agcggggcga tcgcggcgag
     6541 tgccggcgat acatgcagcg agtcctcgcg gtccaggagg agggtcgagg gaaggaatgc
     6601 gacaaacgcg accggcagca cgaaggtcag cagatactcc agcattttcg ggaagatcga
     6661 gaagggatag ttcccataga gattaaagac ctcgttcact gtcgttcgca ccgacatggt
     6721 ctgcaggaac cggaacgaga gcgatcccag tgaaatctgc accgcgccct cgaccagcgc
     6781 cccacccgtg accgccgcca gcaggtacaa caccgtcaac ggcgaccatt ccaccgagat
     6841 tccgaaactc gcggcggtga gcagcatcgc tccgccgatc aggtccccga acgcgttgat
     6901 ccgcaacttg cgggtgaaca gttgcaacag cggcggcatc ggccgcaaca gatagcgatc
     6961 gtattcaccg gtgagcaaca cgcggtcggc ctcgaacatc tgactgaaac aggcgtagaa
     7021 gatgccatga gaggtcagcc gcatcccgta caggaacgtg atctctccaa gcgaccagcc
     7081 accgatgtcg ttgaagcggg caacgacgat ccagaccgtg acaaagccgg tgaactggta
     7141 gagcatgccg ccgatcatgc ttgtgatcgc gtttgcgcgg tactggaact ccgaacgaat
     7201 cgacgccgac gccaaggcgc caagaatggc tatccgggtc atctcacccg ccctggatca
     7261 cggtacgcag gaaggccctt tcccagaccc accggatgat cccaacgaga gccacggccc
     7321 aaaacacctg gatggcgagc gccgaccaga cggcgcctgt cgccggtgcc ccgacataga
     7381 tcgagaccgg tacgaaggca atcgactgga atggcagtac ctgcacgatc gcgcgcagca
     7441 catccggcat gaaccagagc ggtaccagcg cgccggtcgc aaaattgccg atcagccggt
     7501 acatcatctc gaagccggtc atctccaatg tccagaagct gaccagccca atcaccatgt
     7561 tgagctgcac agcgatgacc caggcgagca ggaagctgac cacataggcc agtcccgcat
     7621 tgaacgatgc cggcggctcc agctccccga cgatcagcgc taccggcacg gagacaacca
     7681 ccatcggcag caggccgacc atgtcaccgg cggcacgcgc gatcagctgg cttggatagc
     7741 ccaccggccg gctaagatca aaaccaatct ggccttcgcg gatgcgctcc tgaattgttc
     7801 ctgacacctg gggacgcaaa aagtagagct ggaggtttgc gatcgtgagg tagacgagcg
     7861 tctgctggat gccgatgccg ccaacctccg tctgccctcc gtaggccgct cgccagacga
     7921 tagtgagcag gtagatcgtg acgccggtaa ggaggatccc catcagcaca ttgaagcgat
     7981 acatgtactg ccagcgtata ttggcccggc ctatctcgat ccacgccggc aggttggccc
     8041 ggatggtcat gaccgaacct cacccggctt gtgctcatcg atgaccagtg cctcgccatc
     8101 acgcagcgcc ttgtacggga gcgacgatct ctccagatac tcgacagtac gctgtgcatt
     8161 cgcagcgtcc gggtgatctg atttccagtg cgggacaatt ctgaaaggca ccagaccgag
     8221 cgtgagaggc ggtacggccg ggtcgtatcc cgcagggata tcatccgggt cgtccatgaa
     8281 attgataccc ctcagatcgg gaccggcgac acacgccccg gcagaatacc cggcatatat
     8341 cagtctgcgc tgcatcccag gctcaagtgc ggtcgcaaat ccggaactca ccatggcacg
     8401 cgcgagtgcg aaggcgctcc ctcccactac ccatacgaga tccaacgctt ccaaacggtg
     8461 gcgaagtcct tcgttatcgc ggaaatactg ccggagatcc acttcctccg aggagtatcc
     8521 gagacgttcc aaatccctgg cttcgcggtc ccagttcctt gcccgcgtgt ctccaaaaac
     8581 atccagcgcg ttgaggatga tccccgcacg gctgttctgc ccctgactcc gcaagcactc
     8641 gctgttattc cccatgcggt atgaactcag gtacgtcatg tcggtgatgt gctccgtacc
     8701 tcacccgcct cgacgatctc ccgcacgacg ccttcgagat caggctcgac gatggagata
     8761 tcagagacag ggtggcgggc aatgacggcg gtgatcaggt ctgcgacagc aatcagcgcc
     8821 ggatcgaacc ggatcgagag ccgcgctgcg ccctgctcaa cgatctccgc gccaggaagg
     8881 ccgagcggcg ggatctcgtc ctccgtctct cccgccaagg tcacctccag cactcgccaa
     8941 ggggcatagc ggcgtttcag ctcggctacg gagccatcgt aaagcaccct ccccttgtcg
     9001 atgaagatga tccgcttgca gaggcgctcg acatccgcga gatcgtgcgt cgtgagcatg
     9061 atcgtcgtgc ctcgctcgcg attggtctcc tccacgaact gacgcatgcg ctccttggcc
     9121 acgacgtcca gcccgaccgt tggctcatcg aggtagagga tacgcggctc atagagcatg
     9181 gcggcggcga ggtcgcccct catccgctgg ccgaggctga gctggcgcac gggtgtccgg
     9241 aggaacggct ccagacccag tagatcgacc agatgactgt gggagcgcgc ataccgggca
     9301 gcatccaccc ggtaaagctt tccgatcagg cgcaacgagt cctccaatgg cagatcccac
     9361 cagagttggg ttcgctggcc aaagacgaca ccgatctgga gggcgttctg ctcccgcttc
     9421 tcccagggaa ccagtccacc cacgcggact tcaccacccg tcgggaccag gatgccggag
     9481 agcatcttga tggtggtcga tttgccggca ccattcgagc cgagatagcc gatcagctcg
     9541 ccctcgtcga tagtgaacga gacgtcatca acggcacgcg tggtgtcgta ctgctgggtg
     9601 aagagggtgc ggaggccgcc caatcgccct ccgatgcggc gtgggcgccg gaactccttg
     9661 acgagatgat cgacctggat gattggcacg tgatgtcctg gtggctgaac gcggcgggag
     9721 atccgaaccg ccgcattcta tcgtacccat gcccatgcag ataccgggtc gagcctagtc
     9781 ctggactgtg tcctcgggag agtggagacg ctggatggtg gtgatgcggc cctcgtgcac
     9841 cgtaaacgcc atgaagatcg tcgccggggg cgtccgctca ccaggttgac ctccatcaac
     9901 ctgccaggta gcggataccc ttgcaatcac ccggtccccc tcgatccggg tgtccgcgac
     9961 ggtcatccgg atctgcgtat gatgaaccca tccagagact gcctcgcgcc cggtggtcga
    10021 tccgcgtggt ccaacgatcg agaccgtgtc cgcaaccagg cggtctacct tccccatatc
    10081 agcagcgttt accgcatcga tccacgcccg cacgaccgga cgcgattcat tgtcggttcc
    10141 catcgatatt cgatccttcc agcgatgcag ggagaattcg atgccacatc ggcgggcagc
    10201 ccggccagag acgccacaac ggtaccattg gtcacccgca tgcaatgttc tggcactcat
    10261 tggtcgataa aatgactcta tgaacggatg atcgttcgcg ctatcttccc gaggacggca
    10321 cgatgggcag caagtctatt cgcccgccat gcaggactac accgacgcca aggatgacat
    10381 cgtgagcgag attcacggac gcatacggag cgcaacgcca tgaccgccgc gcgtgcgaac
    10441 aggctcgtgc cactggggcc gatcgtccgc ttgcaggtcc aggtagagaa gatcaagacc
    10501 ggtgagggca ttcatcagcg atacacccct gaccagaacc tgcgaaccgt cgaggcactc
    10561 cttctcgatt ccggaggagt ttatgggcga tccctaggtg gagagacctt gccggacgtg
    10621 catcatcgcg accatccgcg ctcacggttc cgcggggcca atggcatctc cctgctcacg
    10681 acggggcatt acgcgaaaat gcgagagcga ttcggtggtc acctcacgga tggcatcgca
    10741 ggcgaaagcc tgttggtgca gtgcgatggg gtcatgacgc tcgacgccct cgccaacggc
    10801 attgtgatcg gcgagggtga agatgctgtc agcgtcgaga cgtgggagat ggcccatccc
    10861 tgcacgcctt tcagccgctt cgccctgcag ttccccgacg accagaagcc agatcggcgc
    10921 atcaccgagg cgctgcaatt cctcgacgac ggcacacggg gcttcaacgg gctctatcac
    10981 ggtcagagcc cgaatccggt ggagatccgc cttggcgata tggtttatct acgcgcttga
    11041 tagatgtgcc gtgagcatag ggctgacacg gcttgcggtt tcggccatga ggattcaacg
    11101 ccaagcgcgt ggcgcacaca ctgaaaccca gaatttggcc aaggtcggct tcgcctggga
    11161 ttcatcgctt cgctctgaat gacagggtat tgggctgaaa cccagatagc tcacaagggc
    11221 ttgacatact gctcagccac accgacgtct tggtgctgcg ctcaccctac gagcccgaac
    11281 gcttgagctc cgggtgcgca gccatatact gccgaatcgc ctcgggatag agttcacact
    11341 cagccgcgaa gacgcgtgcc gccagcgatt gggccgtatc ccccggctcg accgggactt
    11401 ccgagcgcag caatggcggt ccgtcatcat acacgtccgt cacgacgtgg acggtcgccc
    11461 cggaaacgcg atcacccctg gcgagcacgg cctcatgcac gcgatcgccg tagcgaccct
    11521 tccccgccgc gtaggccgca gcatcgggca acagtgcggg atgaatattc aggatgcggc
    11581 cttcccaacc gggaaagacg aggagacgcc gaagaaaccc ggccatgatc agcaaatccg
    11641 gttgatgagg agccagcaca tcgtacacgg cctttgaata gttccctacc t
//
LOCUS       DASYWM010000049         5900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_57729, whole genome shotgun sequence.
ACCESSION   DASYWM010000049 DASYWM010000000
VERSION     DASYWM010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57729"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..711)
                     /locus_tag="VGR29_01890"
     CDS             complement(46..711)
                     /locus_tag="VGR29_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEV2072369.1"
                     /translation="MSEYAQAFLLGNGAILGNVCVLPLYPGFIAFLGGTASTDQAGRS
                     RSLLGLVVFAGMMTVMLAVGWVLFELNQTFSSIFTWFLPAVFATVIVLGIMMLVGKNP
                     FARMTSFQTPVVRSPLVTAFLYGMVLGPMTLPCTGPLILSTFVLGVGDVGSLIEGILY
                     FVIFGLGFGWPLVALPFLTTPVQRHMTRWVAQHYAVVGRLSGALLIAVAMFGFWADVV
                     PSL"
     gene            complement(708..1328)
                     /locus_tag="VGR29_01895"
     CDS             complement(708..1328)
                     /locus_tag="VGR29_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072370.1"
                     /translation="MTRWMMTRLLGCIVLLGLSTAPAAMAQSATPPSDDGRPACAAAP
                     QDGTPEEGAETDLAEWQTLTMTDVRTGEDFAVGDFLGCTVYVETMATWCINCMIQMEN
                     VAAALPDLDPDQHVVVSISIETELASEDLAKYADNSDFDWVFSVASPEMLKAIVDAFG
                     RDALVPSITPHVIVYPDGTAGSLQTGGASPEDIVDMMNETSEASLR"
     gene            complement(1488..2333)
                     /locus_tag="VGR29_01900"
     CDS             complement(1488..2333)
                     /locus_tag="VGR29_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV2072371.1"
                     /translation="MGITRSQVAAVPFVERTDYEEPSIFLPENMLREARRQRGLSHGN
                     VPEVCLLDPDGDIVRYLIREERAQRSASWACYHTELWEATNDHVRIGIVGGAVGASFA
                     VLVAEELFASGCRFLVSLSSAGQIAADLNSPCVILIDRALRGEGTSHAYLPPAPTVDA
                     DQDLVAAVEVSLEHFAVPTRRGISWTTDAPFRETATALATARAAGASVVEMEAAALYA
                     FAAACHRPVICFAHVTNTMGVGEADFEKGPADGALQALRVLDAATRAWSPYRQQGEHR
                     EQDQP"
     gene            complement(2556..3059)
                     /locus_tag="VGR29_01905"
     CDS             complement(2556..3059)
                     /locus_tag="VGR29_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072372.1"
                     /translation="MYKVTPSWERDDDARIEPAVELLPPPCFDDRGIVPALAFWRGLA
                     LCLATEYRSLARADLGDPPAPRVNTGGDHDDYFRRLIPEGPVGEAFTGFLTGAPAPVT
                     RIDLHAIRDVRMLADGRVGALVTLVDPDAPGPEEADLRFVVFVMVGDRWLIDEVIELE
                     TTGTPIP"
     gene            complement(3052..3252)
                     /locus_tag="VGR29_01910"
     CDS             complement(3052..3252)
                     /locus_tag="VGR29_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072373.1"
                     /translation="MRYTTTDPLTGSEAIVEHQPDGTYTVSLSVPAYDGTDDAEALKM
                     QVRAAHQVFLALDAIARAMSNV"
     gene            3690..3932
                     /locus_tag="VGR29_01915"
     CDS             3690..3932
                     /locus_tag="VGR29_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072374.1"
                     /translation="MHKPSAPSIGGWLFILYMTTVVGGLISAMASDGSPAAFAGSFAA
                     ALVLSFIVRGVGAVAIRPRGSSNEDTTASRTWYEPD"
     gene            complement(3968..4384)
                     /locus_tag="VGR29_01920"
     CDS             complement(3968..4384)
                     /locus_tag="VGR29_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072375.1"
                     /translation="MAVDLLSPEGGTAAVEDPSSIPGILTGGDLSRHVRAFSDAITHY
                     EQVAHGDAGATMAKRYLVTSTDRETNGVVIEGPFRTEHAALSFAESWAEAFYGGDDVT
                     WYESGGPGEYQVRRILEDGSRTDPLIRISVEEGPAI"
     gene            complement(4859..5080)
                     /locus_tag="VGR29_01925"
     CDS             complement(4859..5080)
                     /locus_tag="VGR29_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072376.1"
                     /translation="MIDLRTRALYAFAAVCHRPVVCFAHVTNTMGVGEGDFEKGPADG
                     ALQALQVLGAVTRAWSPYRQQKEHVNPQS"
     gene            complement(5100..>5900)
                     /locus_tag="VGR29_01930"
     CDS             complement(5100..>5900)
                     /locus_tag="VGR29_01930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2072377.1"
                     /translation="GVFPAALDPGRTDTVASHPGLSYGGLVHNGALQGAAMLEVLQGI
                     SGMYREMDLRYLRYKVVPTIYHVVPSADDLYALFRLGAVRYRCDLSAAVDLDSRRPPS
                     KLRLRNLGMARRAGVRVAYGQQYLEPFWAVLEENLRTRHNARPVHTLEEITCLQSKFP
                     EQIRCIVGTLDDRVVAGVVLFHSPRVAHVQYSASTAAGNSVGASTMVMDYATGQSADL
                     GARYFDFGISNEQEGRILNEGLYRFKTSFGAGGVVHEFYEVELERGYA"
BASE COUNT         1237 a         1799 c         1701 g         1163 t
ORIGIN      
        1 agggtacggt agggcgttcc ttacctggtg ttctcattcc actccttaca ggctcggcac
       61 gacatccgcc cagaacccga acatcgccac ggcaatcagc aatgcaccag aaagtcggcc
      121 aacaacggca tagtgctgcg ctacccagcg tgtcatgtga cgctgaactg gtgttgtcag
      181 aaacggcagc gccaccaacg gccagccaaa ccccagcccg aagatcacaa agtagaggat
      241 tccctcgatc aatgatccga catcgccaac ccccagcaca aaggtgctga ggattagggg
      301 accggtgcat ggcaaggtca tcggccccag caccatccca tagagaaacg ccgttacaag
      361 ggggctacgg accaccggcg tctggaaaga tgtcatgcgc gcaaaggggt tcttgccgac
      421 aagcatcatg atgcccagca caataaccgt ggcgaacacc gcagggagga accaggtgaa
      481 aatcgaactg aaggtctggt tcagctcaaa caacacccag cctactgcca gcatcacggt
      541 catcatgccg gcaaacacca cgaggccgag caacgaccgg cttcgcccag cctgatcggt
      601 gcttgccgtg ccgcccagaa acgcgatgaa tcccgggtac agcggcagga cgcacacatt
      661 ccccaaaatg gcaccattgc ccagaaggaa cgcctgggcg tactcactca tcgaagcgag
      721 gcttcgctgg tttcattcat catgtcgacg atatcctcgg ggctggcgcc accggtctgc
      781 aggctgcctg ctgtcccatc cggatagacg atgacatggg gggtaatcga cggcaccaga
      841 gcgtcccggc caaacgcgtc aacaatcgcc ttgagcatct cgggtgatgc gaccgagaac
      901 acccagtcga agtccgagtt gtcggcatat ttcgccagat cctctgatgc cagctccgtt
      961 tcgatgctga tggataccac cacatgctga tctggatcga ggtccggcag ggccgccgcc
     1021 acgttttcca tctggatcat gcagttgata caccaggtcg ccatcgtctc cacatagacc
     1081 gtgcatccaa gaaaatcacc gaccgcgaag tcctcaccgg tgcgcacgtc cgtcatggtc
     1141 agggtctgcc attcagccag atcagtttcg gccccttctt caggagtacc atcctgaggc
     1201 gccgccgcac atgcggggcg accatcgtca gacggcggtg tggcgctctg cgccattgcc
     1261 gctggtgctg tggagaggcc cagcagcacg atgcagccca gcagccgcgt catcatccac
     1321 ctcgtcatgt ccttggttct ccttgtccgc acagcgctca tcagtctgtc gcttgtcgtg
     1381 cgtcgcgcca caacacgtct acctgcatcc tcttgaattg ccctctctcc aggcacgacc
     1441 cactcgtgca gtgttgccga cagagaccgg cgtggatgta agctcgctta cggttgatcc
     1501 tgttcgcggt gttccccctg ctgacgatac ggtgaccatg cccgtgttgc agcatcgagc
     1561 actcggagtg cttgcagggc accgtccgct ggacccttct cgaagtcagc ttctccgacg
     1621 cccatcgtgt tcgtcacgtg cgcaaagcag ataaccgggc ggtggcaggc tgcagcaaac
     1681 gcatagagcg cagccgcttc catctccacg acgcttgcgc cggccgcgcg agcagtcgcc
     1741 agcgctgtgg ccgtttcgcg aaagggggca tccgtcgtcc aggagatgcc acgacgggtc
     1801 ggcaccgcga agtgctccag ggaaacctcg actgccgcca ccaagtcctg atcagcatct
     1861 actgtgggcg ctggcggcag gtaggcgtgg ctggtccctt ctccgcggag cgcccggtca
     1921 atcaggatga cgcatggcga attcaggtca gcggcgatct ggccggcgga cgacaggctg
     1981 accaggaatc ggcaccccga ggcaaacagc tcctcggcaa ccagcaccgc aaacgacgcc
     2041 ccgacagcac cgccaacaat gccaatccgt acatggtcgt ttgtggcctc ccagagttcg
     2101 gtgtgataac aggcccagga cgcggaccgc tgcgcccgtt cttcccggat caggtagcgc
     2161 acaatgtctc catcgggatc gagcaagcag acctcgggga cattgccatg agatagaccg
     2221 cgttgacgac gtgcctctcg cagcatgttc tcgggcaaga agatcgaggg ctcttcataa
     2281 tccgtacgtt ccacgaacgg gactgccgcg acttgggagc gagtgatgcc catgttgatt
     2341 tgatgtccta tcgtgctaaa gaatgaatcg ctttacgaaa tgagcacacc atggaagctc
     2401 agaatggcag cgtgcggcac ggtcgtcctt tgccaacgca tccagaatca atagttcttc
     2461 ggtcactaga gactgctgcg tctcctagct gcggggccag ctgagcatga ttgcctgctt
     2521 gtcttcggcg tggatttgac tcctgccgtc tcttgctacg gtattggcgt gcccgtcgtt
     2581 tcaagctcga tgacctcgtc gatcagccaa cggtcgccca ccatgacgaa gacgacaaag
     2641 cggagatctg cctcctcggg accgggagcg tcgggatcga cgagcgtgac cagggcaccc
     2701 acccgcccgt cggccagcat ccggacgtcc cggatggcgt ggaggtcaat ccgcgtcacg
     2761 ggtgcgggcg cgcctgtgag gaagccggta aaagcctcgc cgaccggccc ttccgggatt
     2821 aagcggcgaa agtagtcgtc atggtcaccc cccgtgttta cccgaggagc tggcggatca
     2881 cccagatcag ccctcgccaa agagcgatac tcagtggcaa ggcatagtgc cagccctcgc
     2941 caaaaagcga gggctggcac tatgccacgg tcgtcgaagc aagggggcgg gagtagctcg
     3001 accgctggct cgattcgggc atcatcatca cgctcccagc tgggagttac cttatacatt
     3061 ggacatagcc ctggctatcg catcgagggc aaggaacacc tggtgtgctg ctcgtacttg
     3121 catcttgagg gcttccgcgt cgtcggtccc atcataggcc ggtaccgaga gcgagaccgt
     3181 gtaggtgccg tccggctggt gctcgacaat ggcttcgctc cccgtcagag ggtcggtggt
     3241 cgtgtagcgc atgctgacat gaagctcctg tactcgcgtc gggtatgaaa ttgatgtaac
     3301 aaacgatgct gccgtcggta gaagtcgaaa gagtctgtaa caacagaagg tacggagatt
     3361 gtaccattcg gtacagtcta ggtgaattta taaggcacaa cccgtgccac atattttttc
     3421 aagcacgtag agatacaacc acggcccccg aggtatcagg agccgtgtcg tctgcttgcg
     3481 ttgcgtgacg gggctgctgg gccatggtgg acatcgtgct cgaccgggaa gggcggctgg
     3541 tgatcaagcc ggggatgaac atgcggtaaa ggtggtcctg aggcacggtg tttccgtttc
     3601 cccatggatg accgcactgc cctatgaatg atcgtgcgtg tcctcgcgcg cgcgacactg
     3661 actcccacag gggtcaggag gcctatcgta tgcacaagcc tagtgctccg tcgatcgggg
     3721 gatggctctt cattctgtac atgacgactg tggtcggtgg tctcatctcg gccatggcca
     3781 gtgacggcag cccagcagcg ttcgccggca gctttgctgc ggctctggtg ctctcgttca
     3841 tcgtgagggg tgtgggtgct gttgccatcc ggccacgtgg gagcagcaat gaggacacga
     3901 cagcgtcccg cacatggtac gagccagatt aggaactatc gttgcgtggg ggtgatcctg
     3961 gcgcgttcta gatagccggt ccctcttcga ccgagatgcg tatcagcgga tcggttcgtg
     4021 agccgtcctc gagaatacgc ctcacctggt actcacctgg accgccactt tcgtaccacg
     4081 tcacatcatc gccaccataa aatgcctcag cccatgactc ggcaaacgaa agggcagcat
     4141 gctctgtgcg gaacggtcct tcaatcacca cgccatttgt ctctctgtcg gttgacgtga
     4201 cgagatatcg ctttgccatg gtggctcctg cgtcgccgtg cgccacctgc tcgtagtggg
     4261 taatggcgtc ggagaacgcc cggacgtggc gcgagaggtc accacccgta aggattccgg
     4321 gaatgctcga tggatcctcc acggcggcag tgccgccctc ggggctgagg agatccaccg
     4381 ccacgccgat gtaccccgcc ttgtccgatc gccgggaggg gcggctttcg gcaccgactg
     4441 atctcctgtc gggatcaata tgcgcctagg catatggacc acctcgagtt cttctttctg
     4501 atgacagagc ccggataagt agaagcacat gacgcacaaa tctgcgtttg cgaaatacca
     4561 atgattgcgc cgaccggtcc acgttctgag cacggagctt cgggtgatac gttgagggac
     4621 cgacctaggc ggtcgagggc ttgtctgctt tgtagtgacg cgttgcaagg atagcagcgc
     4681 ggaaaccaat gattcctgtc accgcaggaa ttcctagact ctcagagtta ggattcagga
     4741 acgagccgga actggtcctt ggcatcgcgc tgacaatcag cagagtcacg gatctgaccc
     4801 ggttccctaa aggtcagtcg ctcgcatcga ttgttgtagt aatgcagatc ggactgcctc
     4861 acgattgagg gttcacgtgt tctttctgct gacgataggg tgaccatgcc cgtgtgactg
     4921 caccaagcac ctgaagcgct tgcagggcac cgtccgctgg gcccttctcg aagtcacctt
     4981 ctccgacccc catcgtgttc gtcacatggg caaagcagac caccgggcgg tggcagaccg
     5041 cagcaaacgc atagagcgca cgggtccgta ggtcgatcaa gttgcagcgt tcaatctgtt
     5101 taagcgtacc cacgctctaa ctcgacctca tagaattcgt gcacaactcc ccccgccccg
     5161 aagctcgtct tgaatcgata aagcccctca ttgagtatcc ttccttcctg ttcgttacta
     5221 atcccgaaat caaagtagcg cgcccccaag tccgcactct gccctgtagc gtagtccata
     5281 accatagtcg aggccccaac agaattgccg gcagcggtgg aggcgctata ttggacgtgc
     5341 gcaactcgag gcgagtggaa caagacaaca ccggcgacca ctcgatcgtc taaggttcca
     5401 actatgcatc tgatctgctc gggaaatttg ctctgtaggc aagtaatctc ttcgagcgta
     5461 tggaccggac gagcattgtg tctggttcta agattctcct cgagcaccgc ccagaaaggc
     5521 tccagatact gctgcccgta tgcaacgcgt acgcctgccc ttcgagccat tccaagattc
     5581 ctgagccgaa gcttgctcgg tggtcggcga gaatcaaggt ccacagcggc ggacagatca
     5641 caccggtacc gcaccgctcc tagacggaac aaggcgtaaa ggtcgtcggc cgagggaacg
     5701 acgtggtaaa tagtcggaac caccttgtac cgcaggtatc gcaaatccat ctcgcgatac
     5761 atccctgaga tgccctgcag cacttccagc atcgcagctc cttgaagggc cccattgtgt
     5821 acaaggcctc cgtaggagag gccagggtga cttgccacgg tatccgtccg accgggatcg
     5881 agagccgcag ggaacacgcc
//
LOCUS       DASYWM010000050         7119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_376977, whole genome shotgun sequence.
ACCESSION   DASYWM010000050 DASYWM010000000
VERSION     DASYWM010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376977"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VGR29_01935"
     CDS             complement(<1..434)
                     /locus_tag="VGR29_01935"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEV2072378.1"
                     /translation="MGSSPTPRPRPRPRTLPRRNTATYGGLWPVATGVAIAALLGMLV
                     WNINLRGDVDDLEEQLSSLVRDNAALRENANATVYQLTPTSDAPDNAHAQAWFSVQGS
                     GVLSVANLPVLSEGRSYQLWYSTDSPTTPIPGGTFRVDDTGQG"
     gene            826..2124
                     /locus_tag="VGR29_01940"
     CDS             826..2124
                     /locus_tag="VGR29_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072379.1"
                     /translation="MACVLLVLVPFSGAVSAQNAQPQPTQDPAQLVPEDPTAPQADDE
                     SAPTGQDAPDATPEPATSNTDNNSNNAQVTRSAQRADEAAGPGFPAVLAHGLAYVDGG
                     EVVWQVRDVEIPAADDAESSITEESIHLQRDGSSIIRNDVTGKRAKTDRGEAYFRAAG
                     DGYTLSAEGGESLAWVFELVDPDDVAIDAFYESPEIDDLDEGVYDMMLTRYVLQSDES
                     VDLPDHSGAGLIMVSSGEIEVDADGERSALSQSDGQTIQDEATITNTTGEPAVFYYAY
                     LGDEVGDATAGAAQNQADQPSTTTATDEPAEDAVDDPEAAAADAETVEEASEADAGSA
                     ETGEAPITDDAGNFLTSINVTADTEIYLIITVDGLTVFDGTLPAGASSGPVVGTTFTV
                     YTSSGVNTTFTNACGETFKMGYEEGEATYVLEASASSCAP"
     gene            2438..2875
                     /gene="mscL"
                     /locus_tag="VGR29_01945"
     CDS             2438..2875
                     /gene="mscL"
                     /locus_tag="VGR29_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV2072380.1"
                     /translation="MKSLGSEFKEFIMQGNVVSLAVAVVIGAAFGALINSFVADMITP
                     LIAAFGGQPDFSNIAFTINGSRFMIGNFINALVAFLIIAAIIFVFVVKPMNALIERSK
                     TEDPVDPTVTKCPYCFSNVPVEATRCHACTSDLSDSHAPAARV"
     gene            complement(3041..3307)
                     /locus_tag="VGR29_01950"
     CDS             complement(3041..3307)
                     /locus_tag="VGR29_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072381.1"
                     /translation="MIATPTPTHSSSTFLSAGQKFLSDDLFSSGGSQVLTVARLARDR
                     FGLRHVVLRNDDGREISAFVEQVELAIELGNLRPVVEALEGIAC"
     gene            3828..4736
                     /locus_tag="VGR29_01955"
     CDS             3828..4736
                     /locus_tag="VGR29_01955"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEV2072382.1"
                     /translation="MLNTRELDDSNEDSGLMALVSDYVALTKPGIMALLLVTTLGAML
                     IAASGAPPFGLVLLTMAGGWMSAAGANVLNCVIDRDIDARMPRTRKRGTASGRIPAVN
                     ALVFGLTLTLGGFLLLGVLVNWLAGFLALVGNVYYVLVYTLWLKRTTPQNIVIGGAAG
                     AMPPLVGWAAVTGTLSPAAWILFAIIFYWTPPHFWALALLKQGEYGRVNVPMLPVVYG
                     EEHTRRQIFLYTVMLAAVSLMLTPFGMSWIYLLGAVLLAAWFLRLTLKLLLSPSKALA
                     RKTFFYSIWYVAGLFAVMATDRLILG"
     gene            complement(4913..6310)
                     /locus_tag="VGR29_01960"
     CDS             complement(4913..6310)
                     /locus_tag="VGR29_01960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEV2072383.1"
                     /translation="MNITIFGTGYVGLVTGACFAESGNDVCCVDVDEKKIEQLRKGIV
                     PIYEPGLSELVEQNVAAGRLSFTTDAREGIDHGRFIFIAVGTPANEDGSSDLKYVEAV
                     ATTIGDLLTRPAIIINKSTVPVGTADRVREIVCGRLEERNVEVPFDVVSNPEFLKEGA
                     AIEDFQRPDRIVVGAESPEAIEAMRQLYAPFNRNHNRFMVMDIRSAELTKYAANAMLA
                     TKISFMNEIANIAERVGANIENVRVGIGSDSRIGYNFIYAGIGYGGSCFPKDIRALVN
                     TAAMHDYDARLIAAVEDVNQDQKRVLLDKIYEHFGSNLAGRTFAVWGLAFKPKTDDMR
                     DAPSREIIEGLWSRGATVRAYDPEAMQSARSIYGERNDLVLCEQRDDALEGADALVIC
                     TEWRQFRSPDWELLRSTLREPVIFDGRNIYDPDRVAREGFTYYGVGLGGKAASTAHND
                     IGSAAKPDPHLITAD"
     gene            complement(6636..>7119)
                     /locus_tag="VGR29_01965"
     CDS             complement(6636..>7119)
                     /locus_tag="VGR29_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV2072384.1"
                     /translation="EIAHPDHDTHYEAEIVVVIGKKAKDVSEGEALDYVLGYTAGNDV
                     SDRVEQYADGQWIRGKGYDTYLPLGPAIETELNVSNVKVQSRLNGETRQSESTKSLIF
                     RVPFLVSFLSGVMTLNPGDIIMTGTPHGVGPMKEGDVIEIDVEGVGVLRNPVKNRVRA
                     "
BASE COUNT         1420 a         2025 c         2163 g         1511 t
ORIGIN      
        1 ccctgtcccg tatcgtccac ccgaaacgta ccaccgggaa tgggggtggt cggggaatct
       61 gtggaatacc agagctgata ggaacgacct tccgagagaa caggtagatt ggcgaccgac
      121 aggacgcccg agccctgtac ggagaaccat gcctgggcat gggcgttatc cggtgcgtcg
      181 gaggtggggg taagttggta caccgtggcg ttcgcgtttt cgcgcaacgc ggcattgtct
      241 ctcaccaagg aggagagttg ctcctcaagg tcgtcgacat ctccccgaag gtttatgttc
      301 cagaccagca tgccgagcag ggcagcgata gcgaccccgg tcgcaaccgg ccaaaggcca
      361 ccatacgtgg ctgtattccg cctcgggaga gtacgcggtc gtggtctcgg gcgtggtgtt
      421 ggggatgagc ccatctgacg atcaaactcc caatgatgac ggacgcggct gcgaagggcc
      481 tcggccgcct cggggaatgt ttcctccccg ggtaaactgg tgtaaactgc gtacgatcag
      541 tgcggacgcc tccggccctc gtagttgatc tggctgaggg ggaacgcctc ctgaatgata
      601 caagtggaca tcgtaacgga cgcataattc tgagggcgga aagcgactct gtattctgcc
      661 gtcgcagacg ttcattgctg gtgcacgagg tgcgccggtc accacctgat ttatgatgga
      721 atcagatcga agggaagccc atcgatgaat cgttcttcca tgaatgctat gagaactgct
      781 tcctctaggg ggagggtcag aagcatccgt ttctggatgg cggtcatggc gtgtgtcctg
      841 ttggtgcttg tgcccttcag tggcgcggtt tccgcccaga atgctcagcc ccagccgaca
      901 caggacccgg cgcagctggt acccgaagat cctaccgcgc cgcaggcgga tgatgaatca
      961 gcgccgactg ggcaggatgc tcctgacgcg acacccgagc cggcaaccag taatacggac
     1021 aacaacagca acaatgctca ggtaacccgg tcggcgcagc gtgccgatga ggcggctggc
     1081 ccaggatttc cggctgtctt ggcgcacggg cttgcatacg tcgacggcgg cgaggttgtc
     1141 tggcaggttc gtgacgtcga gattcctgcc gcagatgacg ccgaatcctc gataacggag
     1201 gaatccattc acttgcagcg tgatggatcc tcgattatcc gaaacgatgt cacgggcaag
     1261 cgtgccaaga ccgatcgcgg tgaggcgtac ttccgagctg caggtgatgg ttacaccttg
     1321 agcgctgagg gtggcgagtc gcttgcctgg gtgttcgagt tggtcgaccc tgatgatgtg
     1381 gccattgacg ctttctacga gagccccgaa atcgacgatc tcgacgaggg tgtctatgac
     1441 atgatgctga ctcgctatgt gttgcagtcc gacgagagtg tcgacctgcc ggaccacagc
     1501 ggggccgggc ttatcatggt cagctcggga gaaatcgagg ttgatgctga cggagagcgc
     1561 agtgcattgt cacaaagcga tggtcagacc atacaggatg aggccacgat caccaatacg
     1621 acgggtgagc cggctgtctt ctactatgcc tatcttgggg atgaagtagg ggacgccacc
     1681 gcgggcgctg cccaaaatca ggcagaccaa ccgtcgacca cgaccgctac agatgagccc
     1741 gccgaggatg cagtggacga tcccgaagcg gcggctgccg atgccgaaac cgtcgaggaa
     1801 gcgtcagagg cagatgcagg aagcgctgag acaggcgaag cgccgattac cgacgatgct
     1861 ggcaacttcc tgaccagcat caatgtcacg gccgatacag agatctacct catcattacc
     1921 gttgacgggt tgacggtgtt cgatggaacc ctgcctgccg gagccagttc tggcccggtg
     1981 gtgggaacaa cattcacggt ctatacctct tctggggtca acacgacctt caccaatgcc
     2041 tgcggcgaga ccttcaagat gggatacgag gaaggcgaag cgacgtacgt ccttgaggca
     2101 agcgcaagct cctgcgcgcc ctagggcgga tgacagagag cccggcgggc gttccacgag
     2161 cagatggagc ggatagcgta tcgccgggcc gttgtcctac cggcctgcat ctgatcggat
     2221 ttgcaggggc gacctcctgc tcatgtcaca gtggtggccc gtcggcaccg acaggccacc
     2281 actgtggtgt ctcctcgtct caggcactgg acgccagccg cattgtgagt agacgcgtct
     2341 cataggtgct ttctcgtcat aacattcgtc agcatgccgg gatccctgtt gatttccatt
     2401 cctgacggat ggagcgtcaa tcgtgaggag gaggagcatg aaaagtctgg gttcggagtt
     2461 caaggaattt atcatgcagg ggaacgtcgt ctcccttgcc gtcgcggtcg tgatcggtgc
     2521 cgcgttcggg gcgctcatca attcgttcgt cgccgacatg attacgccct tgatcgctgc
     2581 gtttggcggt caacccgact tcagcaatat cgccttcacc atcaacggca gcagattcat
     2641 gatcggtaac tttatcaacg cgcttgttgc gtttctgatc attgcagcca tcatctttgt
     2701 cttcgtggtg aagccaatga atgcgctgat agagcgatcg aaaaccgagg atccggtgga
     2761 tccaacggta accaaatgcc cgtactgctt tagcaacgtt cccgtcgagg cgacccgttg
     2821 tcacgcctgc acctcggacc tgagcgactc tcacgcacct gcggctcggg tctaggcaaa
     2881 tcgcttggcg aactatccca gctggcaggt caatagggcg gcatgggata agcggtggtg
     2941 actgtcacac attgatgtcc cagcctgcgt cgaaaacgaa acaatcgcgg caccggaccc
     3001 tccggtgccg cgattgttgg ttgtgatatg gcagtgcccc ctaacaggcg atgccctcaa
     3061 gagcttccac tactggccgg aggtttccaa gttcgattgc cagctccacc tgctcaacaa
     3121 aggcgctaat ctcccggccg tcatcgttgc gaaggacgac atgtctgaga ccgaatctat
     3181 ctcgggctag ccgggcgacg gttagtacct gcgatccgcc cgaggagaag agatcgtcgg
     3241 aaaggaattt ttggcccgcg gaaagaaagg ttgaggagga gtgggttgga gtcggggttg
     3301 cgatcatgtt gtatctccat gataccgggg ccacgtcgtt gtggcgcctg aatgtgtcga
     3361 acgctgaacg gtgcttcctc ccgggaagcg tgtggccgga tcgtatccag tcacgaggca
     3421 caatcctcgc cttaggcacg agtgcttcat aaagaacctc gtgacagatt tcacacattc
     3481 tactaatacg acggacctcc ggccctgtca atacatcacg gggaggattt cgggtggagc
     3541 aagaagggga tgcgccccga tgcggcacga cgtcactcga tggtgtggga tatgcgctgc
     3601 atggtgggta gaataacgag acgtgcgcag ggagctgctg tactccgtcg cgtgtccccg
     3661 tatcattagg cattaagggc ggcaactcca atgccaggta atgacttttg gggtgtgtgt
     3721 cacctgcttc gggatcccga ttgcgtccca cgaattgccc aagaggttga tctgctcaac
     3781 cgcgcactac catgcgtgta tcgcaccggc gtcaggatga cttctctatg ttgaacacgc
     3841 gtgaattgga cgacagtaat gaggattctg gcctcatggc gttggtcagt gactatgtcg
     3901 cgctgaccaa gccggggatc atggccctgt tgctggtaac gacgctgggg gcaatgctga
     3961 tcgcggcgtc aggagcaccg ccctttggcc tggtgttgct caccatggca ggcggctgga
     4021 tgagtgccgc cggtgccaac gtcctcaatt gcgttatcga tcgtgatatt gatgccagga
     4081 tgccgcggac ccggaagcgg ggcacagcaa gtggccgcat ccctgcggtt aacgccttgg
     4141 tcttcggtct gacgctgacg ctggggggat ttctcctgct cggcgtcctg gtgaactggc
     4201 tcgccggatt cctggcgttg gtaggcaatg tctattacgt ccttgtctac accctttggc
     4261 tcaagcgcac gacgccacag aacatcgtca tcggaggcgc cgccggtgct atgcctccct
     4321 tggtaggctg ggctgcggtg acgggaacgc tttcccccgc cgcatggatc ctcttcgcga
     4381 ttatcttcta ctggacaccc ccgcacttct gggcactggc actgctgaag cagggtgagt
     4441 acgggcgcgt caatgtgccg atgttgccgg tggtctatgg cgaggagcac acgcgaaggc
     4501 agatctttct ctacaccgta atgctagctg ccgtgtcgct gatgctgacc ccattcggta
     4561 tgagctggat ctacctgctg ggcgcagtcc tgttggcggc ctggttcttg agactaacgc
     4621 tgaaactgct tctgtcaccc agcaaggcgc tcgcgcgcaa gactttcttc tactcgatct
     4681 ggtatgtcgc cggactcttc gccgtgatgg ccacggaccg cctgatcctt ggatagcgcc
     4741 ggactctcct gaatctggag caaggtgtga agaggagcgg cgccgctttg ttgttgcaag
     4801 aaacggccac ggtggggctt cccataaccc taccgtggcc gtctcagcgc ctctcatggg
     4861 acccgaccag gaaagctgcc ttgggcggtg ctcggcaaac gtgccggctg cgttagtccg
     4921 cggtgatcag atgcgggtcc ggctttgccg cggagccgat atcgttgtga gcggtgcttg
     4981 cagccttacc accgagaccg acgccgtagt aggtgaagcc ttcgcgggct acccgatcgg
     5041 gatcgtagat gttgcggccg tcgaagatga ctggttccct aagcgtactg cggagcagct
     5101 cccaatcggg gctgcggaac tggcgccact cggtacagat aaccagggca tcagccccct
     5161 cgagggcatc atcccgctgc tcacaaagta ccaggtcatt ccgctctcca tagatggagc
     5221 gtgcactctg catcgcctcc ggatcgtatg cccggacagt cgcacccctg gaccacaaac
     5281 cctcaatgat ctcgcggctc ggcgcgtcgc gcatatcgtc cgtctttggc ttgaacgcaa
     5341 gcccccagac cgcaaaggtg cggccagcga gattgctccc gaaatgctcg tagatcttgt
     5401 ccagcaggac gcgcttctgg tcctggttga cgtcctcgac ggcagcgatc agacgggcat
     5461 cgtaatcgtg cattgccgcg gtgttgacaa gcgcgcggat atccttcggg aagcaggagc
     5521 ccccgtagcc tatgccggcg tagatgaagt tgtagccgat gcgggagtct gagccgatcc
     5581 caacccggac gttctcgata ttcgcaccga cccgctctgc aatgttcgca atctcgttca
     5641 tgaaggagat cttggtggcg agcatcgcgt tcgcggcgta ctttgtcagc tcggcggagc
     5701 ggatgtccat caccatgaaa cggttgtggt tgcggttgaa cggcgcatag agttgacgca
     5761 ttgcctcgat cgcttccgga gactctgcac ccaccacgat gcgatccggt cgctggaagt
     5821 cctcaatggc ggcaccttcc ttgaggaact ccgggttgct caccacatcg aacggaactt
     5881 cgacgttacg ctcttcaaga cgcccacaga caatttcgcg cacgcgatcc gctgtaccaa
     5941 ccggcacggt cgacttgttg atgatgattg ccggtcgggt gagaagatcg ccaatggtgg
     6001 tcgcgacggc ttctacatat ttcaggtcag aggatccatc ctcgttcgct ggggtaccaa
     6061 cggcgataaa gataaatcgg ccatgatcaa tgccctcacg ggcgtcggtc gtaaaagaaa
     6121 ggcggccagc agcgacattc tgctccacca actcgctcag ccctggttcg taaatcggaa
     6181 caatgccctt gcgaagctgc tcgatcttct tctcgtcaac atccacgcag cagacatcgt
     6241 tgccactctc ggcgaagcag gcgccagtga cgagcccgac ataaccggtg ccgaaaatcg
     6301 tgatgttcat ctaccggaga tcctcctggg atgcgccgtt tctgggagcc gcagagaccc
     6361 atgtgcgtaa agtagcggcc gaacaagcgc gattatggtc acgaaaaaat atgcctggcg
     6421 ggcaccagtc gagtgtacaa cgccgggttt ctcttttggc ataacgtttg atggtcttcg
     6481 atatcgagat gatgtggtga atcttcggct cgggggaccc tggacaagag tcggttcatc
     6541 gatcacttgt ctgacgatgc aagactgacc gggagtcctg tgcaaagagg ccgccccggt
     6601 tagggcggcc tctcgatcat tgacagggtt accgcttatg cgcgaacgcg gttcttcacc
     6661 gggttgcgaa gcacaccgac gccctcaaca tcgatctcga tgacgtcgcc ctccttcatt
     6721 gggccaacgc cgtggggcgt gccggtcatg atgatgtcgc cgggattcag ggtcatcacg
     6781 ccactgagaa agctgaccag gaaggggacg cggaagatga ggctcttcgt ggattctgat
     6841 tggcgcgttt cgccgttgag gcgggattgc accttcacgt tgctgacgtt cagctcggtc
     6901 tcgattgcag gtccaagcgg caggtaggtg tcatagcctt tgccgcgtat ccactggcca
     6961 tccgcatatt gctcgacacg gtcggagaca tcgttccctg cggtgtaacc gagcacatag
     7021 tccagcgcct ccccttcgga gacatctttc gccttcttgc cgatgaccac cacgatctct
     7081 gcctcgtaat gcgtatcgtg atcgggatgc gcgatctca
//
LOCUS       DASYWM010000051         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_433271, whole genome shotgun sequence.
ACCESSION   DASYWM010000051 DASYWM010000000
VERSION     DASYWM010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433271"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VGR29_01970"
     CDS             <1..430
                     /locus_tag="VGR29_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEV2072385.1"
                     /translation="AVRGLTPLGGPRETSGHKGYGLGLMVEIFCGQLASNTWSHQIGA
                     SYVANAPTEGTGHAFVAWRIDAFRDLDEFKADMDDMLGKLRASKVSDEYPEKQVLIPG
                     DPESDAERRNRALGIPVRRTVLKQLADVAGECGVEFPFEM"
     gene            complement(449..1603)
                     /locus_tag="VGR29_01975"
     CDS             complement(449..1603)
                     /locus_tag="VGR29_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II histone deacetylase"
                     /protein_id="HEV2072386.1"
                     /translation="MVSRRDRRVGLLFDDRYLSHNSGQFLIAYRDQYPFSEPIPHPSS
                     PQIASRTKHLLDFFGYSDVMKRVDAFEATDEMILACHTSEYLARVRDVSKTGGDAGTG
                     APLGRGGERIARLAAGGVAAGVDAVMSGDLHHVYALVRPPGHHAMPDKGMGFCIFGNV
                     AIAACHARRVHGAKKIAILDWDVHHGNGTQDAFYDDPSVLFISIHQEDLFPVGWGALD
                     QTGEGDGTGTTVNIPLPAGSGNRAYMHVMDNIVAPILRQFDPDLILVSAGQDASVEDP
                     LGRMSLTTAGYRAMTGVVRGIADEVCEGRLVVAQEGGYSQHYAPYCSAAIVEGLCDGL
                     GGFQPVEEPYGPRAESMPPSRRIGLDAQAAIDAAIETHERHWVLQEPSVR"
     gene            complement(1885..>2889)
                     /locus_tag="VGR29_01980"
     CDS             complement(1885..>2889)
                     /locus_tag="VGR29_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEV2072387.1"
                     /translation="AMIAAAAGGPLAATAVGAQSQSVDVWVEVATANPAVGCFVDTSI
                     EVRGGGASVSGADVVLTLSDDANNTIYSSDRGVTDDAGIVHLGYDTSATGSGTKTWLE
                     VVVNGSYVGGRTVMVDGGSCAGAPSLLDMSGEVATISDSVVDSPVEQATSSDAVMIPG
                     VSAYLQQRSLSCEYAAVSIATGALGSWVSEYDLEAVTPLSANPHWGYRGNINGVWGNT
                     VDYGIYADALIPGLNQFGFQGHTFYGGPADLMSSIDQGRPTLVWLGLWGDLSHDEYTE
                     DGSRFQLTQAMHVMVAYGYDSGGVYLSDPGTGQYRYYDWGTFDSMWQVMDGMALGVSW
                     "
BASE COUNT          574 a          874 c          907 g          534 t
ORIGIN      
        1 tgccgtccgg gggctgacgc ctcttggcgg tccaagagag acatccgggc acaagggata
       61 tgggcttggg ttgatggtgg agatcttttg cggccagctg gcgagcaata cctggagcca
      121 ccagatcggt gcatcctacg tcgccaatgc gccgacggag gggaccggac acgcctttgt
      181 cgcctggcgg atcgatgcat ttcgtgatct ggatgagttc aaggctgaca tggacgacat
      241 gctggggaag ctccgcgcca gcaaggtatc ggacgagtat ccggagaagc aggtgctgat
      301 tcccggtgat cctgaatcgg atgctgagcg gcggaaccgc gcgctcggca tcccggtgag
      361 acgcacggtc ctgaagcaat tggccgacgt cgctggggag tgcggagtgg agttcccgtt
      421 tgagatgtga gaaacggtgc gagcgtttct accggacgct gggttcttgc aagacccagt
      481 gcctctcgtg tgtctcgata gcagcatcaa tggcggcctg ggcgtcaagg ccaatgcgac
      541 gggaaggtgg catcgactcc gcccgcggtc cgtacggctc ctcaaccggc tggaagccac
      601 caaggccgtc gcagagtccc tcaacgatcg cggcggagca gtagggtgcg tagtgctggc
      661 tgtagccgcc ctcctgcgcg accacaagcc gtccctcgca gacctcatcc gcaatgccgc
      721 gcacaacccc ggtcatcgcc cggtatccgg cggtggtaag ggacatccgg ccaagcgggt
      781 cctccacgct ggcatcctga cccgccgaga cgaggatcag atcgggatcg aattggcgca
      841 ggataggcgc aacaatgtta tccataacgt gcatgtaggc gcgattgccg cttcccgccg
      901 gaagcggaat attgactgtg gtgccggttc cgtcaccctc cccagtctgg tccagcgctc
      961 cccatccgac ggggaagaga tcctcctgat ggatggagat gaagagcacc gaggggtcat
     1021 cgtagaacgc gtcctgcgtg ccgttgccgt gatggacatc ccagtcgagg atagcgatct
     1081 tttttgcacc atgcacccgc cgagcgtgac acgcagcgat cgcgacattc ccaaagatgc
     1141 agaagcccat gcccttgtcc ggcatggcgt gatgtcccgg cggacggacg agcgcgtaga
     1201 cgtgatgcag atctccagac atgaccgcat ctacgccagc ggcaacccca cctgctgcca
     1261 gccgggcgat gcgctcaccg ccacgcccca gtggcgcccc agtgccggcg tctccaccgg
     1321 tcttgctgac gtcgcgcacc cgcgctaggt attcggaggt gtggcaggca aggatcatct
     1381 cgtccgtcgc ctcaaatgca tccacgcgct tcatgacatc cgagtatcca aagaagtcga
     1441 gcaggtgttt ggtacgggag gcaatctggg ggctggatgg atgagggatt ggttcggaaa
     1501 aggggtactg atcgcggtag gcgatcagga actggccact attgtgggac agatagcggt
     1561 cgtcaaagag caggcccact cgtcgatctc gtcgcgaaac cataggacct ccggaattct
     1621 caaggatcag cattggtggg agtatcgcac ggatgagtgc cgcctggtgt cgtcgaaagg
     1681 gggtggcagc tcccagttca ctggcacgga agtcggctgg ttcaagcagc cgctttgcga
     1741 gttcgggttc cctgatgtgt ggaccaggcc caaacattga ctgactggta tgacagctcc
     1801 gcgaggtgtc acggatttct gcgcaagcag aaacggggag gtccatcagg aacgccccgt
     1861 ttctgtgttt catgtcgtgg ccgtttacca ggatacgccg agtgccatgc cgtccatgac
     1921 ctgccacata gaatcgaagg tgccccagtc gtagtagcgg tactggccgg tgccgggatc
     1981 cgagaggtac accccacccg agtcgtagcc gtaggccacc atcacatgca tcgcctgcgt
     2041 caactggaag cgggagccgt cttcggtgta ctcgtcgtgg ctgaggtcac cccaaagacc
     2101 gagccagacg agtgtcgggc gcccctggtc gatcgaggac atcaggtcgg cgggaccacc
     2161 gtagaacgtg tgaccctgga aaccgaactg gttcaggccc gggatcagcg cgtcagcata
     2221 gatgccgtag tcgacggtat tgccccagac gccgttgata ttcccgcggt atccccaatg
     2281 tggattggca gaaagcgggg ttactgcctc caggtcgtat tcggataccc agctgccgag
     2341 cgcgccggtg gcaatcgaaa ccgccgcgta ctcgcagctc agcgagcgtt gctgcaggta
     2401 cgccgaaacg ccaggaatca tgacagcatc gctcgacgtg gcctgttcga ccgggctgtc
     2461 gacaacactg tccgagattg tcgcaacttc gccgctcata tccagcaacg aaggggcacc
     2521 tgcgcaactg cccccatcga ccatcacggt tcggccacca acatacgagc cgttcaccac
     2581 gacctccagc caggtcttgg tgccactgcc ggtggcactg gtgtcatagc ccaggtgcac
     2641 gatgccagcg tcgtctgtca cgccgcggtc gctgctgtaa attgtgttgt tagcatcatc
     2701 ggagagcgtc agcacgacat ccgcaccgga gacgctcgca ccacctccac gaacttcaat
     2761 acttgtgtcg acgaagcagc caaccgccgg attggccgtc gctacttcaa cccagacgtc
     2821 gactgattgg ctctgggcac cgacggcagt tgctgccaag ggccctccgg cagcagccgc
     2881 gatcatggc
//
LOCUS       DASYWM010000052         7411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_95475, whole genome shotgun sequence.
ACCESSION   DASYWM010000052 DASYWM010000000
VERSION     DASYWM010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95475"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VGR29_01985"
     CDS             <1..1245
                     /locus_tag="VGR29_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HEV2072388.1"
                     /translation="VYNLFGMAYCGADVSSFLRRRAEQGFNLLRVRLPVSPFHPPDGY
                     SEWQTRRSWPWGGSEQAPQFDRFNLEYFRTVDKVVQEADGLGLGIEMIMEGWGFEFPF
                     NSRQIFTTEWEELWFRYLIARYDAFASTYFWTPLNEYEYYPNGDWHYTPTADRWQMRV
                     SRWIKEHSAHGHCVAAHNGPTLPPFAQRFAADPEAVDAIMFQEWGTRDEENGWLASGI
                     EESIDASLEGWQGSAVLAEWGYERNPELELKLPSHLYCDEEHTRRGAWRGVFRGLGVI
                     HGFENSWGPWQLLDRDQPGLEALLHLHHFVTTIIPFHQMRPTPDLLQATDLEVGRQPQ
                     ALATPDLGFTAVYLPVGGEVSLQVLNGNDRVDQWFDPRTGSSFPAEGRGDGDRRVFSA
                     PRDDQDGHPCDWILVSRAVSAQ"
     gene            complement(1676..1831)
                     /locus_tag="VGR29_01990"
     CDS             complement(1676..1831)
                     /locus_tag="VGR29_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072389.1"
                     /translation="MFATTSDFRASDQEDDVGFITVNLSAHLRTNDYLESFTEFGYWS
                     LPAYRVC"
     gene            2185..2976
                     /locus_tag="VGR29_01995"
     CDS             2185..2976
                     /locus_tag="VGR29_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEV2072390.1"
                     /translation="MPKASEIAASSTSPRLVPLLSQGITACLFDLDGVLTKTAEVHAK
                     AWKEMFDAFLRKHAQRGDEPFHPFETATDYATYVDGKLREDGVRAFLASRGIDLPEGS
                     PDDPPSAETMYGLGTRKNDLVLALIREQGVDVYVGSMRFVRVVRDAGLRCAVVSASKN
                     TGEVLKAAGIAGLFEVRVDGIVAAAEGLPGKPAPDTFLAAASKLDVDPVNCAVFEDAV
                     AGVEAGRAGNFGCVVGVDRVGQAEVLLSHGADIVVADLGELLEEL"
     gene            2973..5369
                     /locus_tag="VGR29_02000"
     CDS             2973..5369
                     /locus_tag="VGR29_02000"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEV2072391.1"
                     /translation="MIEPGVFPVEPWAVTEPELRLDLLAQTESIFALSNGHIGLRGNL
                     DEGEPHVTPGTYLNGFFESLPLPYAEAGYGYPEEGQSLINVTNGKLFRLLVDDEPFDI
                     RLGTLRRHKRVLDLRAGVLGREVEWVSPAGQTVRVRSTRLVSFVQRAIVAIRYEVEAV
                     DEPARIVVQSSLVANEPLPEHTDDPRAAAALRAPLVGEYYVHHDLKAALGHRTRVSAL
                     RMAAGFDHVLEGPASTVTESESEPDLARVTISTQLEPGQTLKVVKLLAYGWSSVRSMP
                     ALRDQVDAALSAAKRTGWDGLLAEQRAHLDEVRVSADVEIEGDPELQQAVRFAIFQVE
                     QAAARAEQRAIPAKGLTGRGYDGHTFWDMETYMMPVLTYAAPWAARDVLLWRHSTIDL
                     ARDRARELRLAGAAFPWRTIRGEECSGYWPAGTAGFHINADIADAVRRYVAATGDTEF
                     ERGAGLELLVETARLWRSLGHHDAEGSFRIDGVTGPDEYSALVDNNVFTNLMAARNLQ
                     VAAEAASRYPQQAAELGVNKRETDSWRAAAKAIVVPFDHELGVTAQSEGFTRYRQWDF
                     EAMSADDYPLLLHYPYYLLYSSQVIKQADLVLALYLCGDRFDPEQKRRDFEYYEAITV
                     RDSSLSASIQAIVAAEVGHLQLAYDYFRETAFVDLHDLSGNTKDGLHLAALSGTPLVA
                     VAGFGGMRDHGNTLAFAPRLPASLDRLRFRMMYRGRRLRVDMSPGEASYEVLAGDPIE
                     LRHYGDLMKLEEGVPQNRPCPCPPELPEVAPPPGRAPCRQGVGQNGSGSEAAPLGFRP
                     "
     gene            5529..5885
                     /locus_tag="VGR29_02005"
     CDS             5529..5885
                     /locus_tag="VGR29_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072392.1"
                     /translation="MKRPHASDDVRERALAAIDEGRSVADGTAFSRNDPSTIRRWMRM
                     RQRTGSWRIQPRSGGLRRISPAQEAALRAQVFAHPDATLAEHCARWEAMQGMRVGVAT
                     MGRALRRPGLTRNKRP"
     gene            6057..6461
                     /locus_tag="VGR29_02010"
     CDS             6057..6461
                     /locus_tag="VGR29_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2072393.1"
                     /translation="MDGRDVAGHAPPDGLGPGLHFAGALDRSTFDTFVADLLVPSLRP
                     SDIVVLDNLSVHQRAMARQRIEAVGAQLGFGPTSSPDFNPTEQAFAKLKHLLRQTAAR
                     SVDAVLAATQHLSPRMTCQDTHGSYRHAGYTL"
     gene            complement(6505..>7411)
                     /locus_tag="VGR29_02015"
     CDS             complement(6505..>7411)
                     /locus_tag="VGR29_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEV2072394.1"
                     /translation="LLSAVAPAAAQSVSCTDYDAWEWAQSVFESDPQKYDALDPDNDG
                     KACPELPPGFAPAFWTDRIPEDVQEAQIVRIVDGDTFEVLINGVSNRVRIYRADTPET
                     QNEQHCGGSEATAFAEWVLSFNDNPGTVYIEKDTTEKDRYGRELGYIWFEVGGQPYML
                     NHVLINNGYAEDVDYGDRKYDQQFKDAAAFADRHDLGVWGLCGGFDIPLTAGAPAPTQ
                     APAVQQQPPAAVQVPAEPAPPPVQEPAAEVPAAGGCDPNYTPCVPIVSYDLDCGDIGF
                     SVTVIGSDPHGFDGNDNDGLGCESY"
BASE COUNT         1363 a         2285 c         2356 g         1407 t
ORIGIN      
        1 gtgtacaacc tcttcggaat ggcgtactgt ggcgccgatg tgtcgtcctt cctccgacgg
       61 cgcgccgagc agggcttcaa cctcctgcgc gtgcgcctcc ccgtcagtcc cttccaccca
      121 ccagacggtt acagcgagtg gcaaacgcgg cgctcctggc cctggggcgg cagtgagcag
      181 gcaccgcaat ttgaccggtt caatctcgag tacttccgaa ctgtcgacaa ggtggtacag
      241 gaggccgatg gcttgggcct cggcatcgaa atgatcatgg agggctgggg cttcgagttc
      301 ccgttcaaca gccgccagat cttcaccacc gagtgggaag aactctggtt ccggtacctg
      361 atcgctcgct acgacgcctt cgccagtacg tacttctgga caccgctcaa tgagtacgag
      421 tactacccga acggcgactg gcactacacc cccaccgccg atcgttggca aatgcgcgta
      481 tcgcggtgga tcaaggagca ctcggcgcat ggccactgtg ttgctgccca taacggtccg
      541 acgttgccgc cgtttgccca gcggttcgcc gccgatccgg aggcagttga cgccatcatg
      601 ttccaggaat ggggcacacg ggacgaagaa aacggctggt tagcctccgg catcgaggaa
      661 tcgatcgatg ccagcctgga gggatggcag ggatcggctg tgctggcgga gtggggatac
      721 gagcggaacc cggagctgga attgaagctg cccagccacc tctattgtga tgaggagcac
      781 acccgccggg gagcgtggcg aggggtcttt cgcggactgg gtgttatcca cgggttcgaa
      841 aattcgtggg gaccatggca gctactcgac cgggaccaac caggcctgga agcgctgctg
      901 caccttcacc actttgtcac cacaatcatc ccgttccacc agatgagacc aaccccggac
      961 ctcctgcagg caaccgatct tgaggtaggc cgccagccac aggccctggc gacgcctgat
     1021 ctggggttca cggccgtcta ccttccggtc ggcggagagg tttccctgca ggtgctcaat
     1081 ggtaacgacc gcgtagatca atggtttgat ccgcggacgg gcagctcgtt cccggccgaa
     1141 ggccgggggg acggggatcg ccgagtgttt tccgcccctc gagatgacca ggatgggcat
     1201 ccctgtgact ggattctggt gtcccgcgct gtgtcagcgc agtagcaagc gaggagcgac
     1261 aagtggaatt acggttacct atgattggcg catagtcccg gagaagcacc gcttctgcag
     1321 cagaacgacg gtcaccggac gggtcgtgct agttcgagtg aatggctttg gtactggtcc
     1381 cgggtacagc aggcgcccag gacaaccgag gccttcggcg gagcagcgac ctcaacttcc
     1441 tgcgaagggt tcgtcttggc tcggtaccat cgtctcaaat cacgggatca ggcgtcatca
     1501 gtcccggcgg atcgaaactg ccgcttcgac cgttcgtccc gcaccgttca cacgagggct
     1561 ccgagcttct acccatctgc accaaagatc gcactaaagg caagcgacga cgcgagcccg
     1621 ccattcccga tgcacccact cgcccagctg gcgcccaacg aaccattggc gctcttcagc
     1681 atacgcgata cgcgggcagc gaccagtagc cgaactccgt gaagctctcc agataatcgt
     1741 tggtgcggag atgggcagag aggttcacgg tgatgaagcc gacgtcgtct tcctggtcgg
     1801 acgccctgaa atcggaggtc gtcgcaaaca cttagtacag atgcttggcg agcgtccgca
     1861 gtgcggtcac gcgaccggta caaacatgcg ggctggctac cttgcagcgc caaaacacgc
     1921 tactttgcaa atcgcgcggg tgtcaatcac gaaacgtgtg cgcgcacctg tgtgcacaac
     1981 gcctttgcag ccgcgagaat cgaattgtag atatgacggt tacagcgtcc acgaagagtg
     2041 ccattgaacg agcgcttgcc tggtggggtc ccataaagaa cgtgcgcagg acgtgcggcg
     2101 acaagggctc cgcgatatcg gcacaggtct gcactatgct tcgtctcagc atctgcaacg
     2161 ttggtactgc gcaggaggat tgccgtgccc aaagcatcgg agattgccgc atcatcaaca
     2221 tcccctaggc tcgttcccct gctttcccaa ggtatcaccg cctgcctttt tgatctcgat
     2281 ggcgtcctca cgaagacggc ggaggtccat gcgaaggctt ggaaagagat gttcgatgcc
     2341 tttctgcgaa agcatgccca gaggggcgac gagccgtttc accccttcga gactgcgacg
     2401 gactacgcaa cgtacgttga cggcaagctg cgcgaggatg gcgtgcgtgc ctttctcgca
     2461 tctcgcggga tagacctgcc ggaaggatcg cccgatgatc caccgtcggc ggaaaccatg
     2521 tacggtctcg ggacgcgtaa gaacgatctc gtgctcgcgc tcatccggga gcagggcgtc
     2581 gatgtgtacg tgggatctat gcggtttgtc agagtcgtac gggatgccgg attgcgctgc
     2641 gcggttgtct cagcgagcaa gaatacgggt gaggtgctga aggccgccgg aatcgcgggg
     2701 ctgtttgagg tgcgtgtcga cgggatcgtc gccgcagcgg aggggctccc ggggaaacca
     2761 gcacccgata cgtttctggc tgcggccagc aagttggacg tagatcctgt gaactgtgcc
     2821 gtgtttgagg atgcggtcgc cggtgttgag gccggccggg ccggtaattt tggttgcgtg
     2881 gtcggtgttg accgcgtggg acaggcagaa gtcttgctta gtcatggtgc ggatatcgta
     2941 gtggccgatt taggtgagtt gctggaggag ctatgatcga gcctggagtg ttcccggtgg
     3001 agccgtgggc tgttaccgag ccggagctgc gtctcgatct gctggcgcag acagagtcga
     3061 tcttcgcgct gtcgaacggc cacatcggat tacgtggcaa cctagatgag ggcgagccgc
     3121 acgtcactcc aggcacgtac ctgaacggtt tctttgagtc actgccactg ccatacgctg
     3181 aggcagggta tggctatccc gaagagggcc agtcgctgat caatgtgacc aacggtaaat
     3241 tgttccggct cctcgtcgac gacgagccgt tcgatatccg gcttgggaca ctacgccggc
     3301 acaaacgggt gcttgacctg cgtgccggcg tcctggggcg tgaggtggaa tgggtttcac
     3361 cggcgggcca gacggtccgt gttcgttcga ccaggcttgt ctcgttcgtc cagcgcgcga
     3421 tcgtggcgat tcgttatgaa gtcgaggcgg tggatgagcc ggccagaatc gtcgtccagt
     3481 cgtcgctagt cgccaatgaa cccctgccag aacacaccga cgacccccga gccgccgccg
     3541 cgctgcgcgc cccgctcgtc ggcgagtatt acgtgcacca tgatctcaaa gccgcgctcg
     3601 ggcaccggac tcgtgtcagc gctttgcgga tggcagccgg gttcgatcat gtgctggagg
     3661 gcccggcaag tacggtaacc gagtcggaaa gtgagccgga cctggcccgg gtgacgatca
     3721 gcacccagtt ggaacccggg cagacgctga aggtggtcaa actcctcgcc tacggctggt
     3781 cgagcgttcg ctcaatgcca gcattgcgtg atcaggtcga tgctgcgctc tcggccgcga
     3841 agcgaaccgg ctgggacgga cttctcgctg agcagcgtgc acacctcgat gaggtgcggg
     3901 tcagcgctga cgtagagatc gagggcgacc cggagttaca gcaggcggtg cgtttcgcga
     3961 tcttccaggt ggagcaagcc gccgcccgag cggagcagcg agcaatccct gccaagggcc
     4021 tcacgggccg cggctacgac gggcatacgt tctgggacat ggaaacctat atgatgccgg
     4081 tcctgacgta tgcggcacca tgggctgccc gggacgtctt gctctggcgg cactcgacaa
     4141 tagatctggc gcgggatcgt gcacgcgagc tgcgattggc aggtgcggca tttccatggc
     4201 gaacgattcg cggcgaggag tgctcggggt attggccggc cgggaccgca gggttccata
     4261 tcaacgccga tattgccgac gcagtgcgcc gctatgtcgc tgcaaccggt gatacggagt
     4321 tcgaacgggg ggccggcctt gagttgcttg tcgaaacggc gcgcctatgg cgatcgctcg
     4381 gccatcacga cgccgaagga tccttccgca tcgacggtgt gactgggccc gatgagtact
     4441 cggcgctcgt cgacaacaac gtcttcacca acttgatggc agcccgcaat ctccaagttg
     4501 ctgcggaagc ggcgtcccga tatccgcaac aagcggcgga gcttggcgtc aacaaaagag
     4561 agaccgactc gtggcgcgcg gcggccaagg ccatcgtcgt gccattcgac catgagcttg
     4621 gtgtaaccgc gcagtcggag gggtttaccc gttaccgtca gtgggacttc gaggcgatgt
     4681 ccgcggatga ttacccgctg ctgctgcact acccgtatta cctgctctat tcaagccaag
     4741 tcatcaagca ggctgatctg gtcttggccc tctacctgtg tggcgatcgg tttgaccctg
     4801 aacagaagcg gcgagacttc gagtattacg aggctatcac ggtccgcgac tcatcgctct
     4861 cggcatctat ccaggctatc gttgcggccg aggttgggca cctccaactt gcctacgatt
     4921 acttccgcga gacggccttt gtcgatctcc acgatttgtc aggcaatacg aaggatggtc
     4981 tacacctcgc ggcactgtcc gggacaccac ttgtcgcggt ggccggtttc ggtggtatgc
     5041 gcgaccatgg aaacaccctt gcctttgccc cacgcctgcc tgcgtcactc gaccggctac
     5101 gattccgaat gatgtaccgc ggccggcgcc tgcgcgtcga catgagccct ggtgaggcaa
     5161 gctatgaggt gcttgccggc gacccgatcg agctgcgcca ctatggtgat ctaatgaagt
     5221 tggaggaagg tgtaccccaa aatcgaccgt gcccttgccc acccgaactg ccggaggttg
     5281 caccaccgcc cgggcgtgca ccgtgccgcc aaggggtggg tcaaaatggc agtggcagtg
     5341 aggccgcacc actgggcttc aggccatgag gtttcctgcc cggcggccac cctgtctatc
     5401 actcaccttc gtggcgtcgt aggcccaagc tcccgctccc cccttgcctg cgcttgcgac
     5461 agccatcttc cccgccccag agttagagcg gctatcgaaa gaagtgcgca tctggcagac
     5521 tggagggtat gaagcgcccg cacgcctcgg atgacgtccg tgagcgagca ctcgccgcca
     5581 tcgacgaggg gcggtcggtc gctgatggca ccgcgttctc tcggaacgat ccctcgacca
     5641 tccgccgctg gatgcggatg cgccagcgga ccggttcctg gcgcatacag cctcgctcgg
     5701 gaggcctccg ccggatcagt cccgcccagg aggcagcctt gcgtgctcag gtgttcgccc
     5761 acccggacgc gacattggcg gagcattgtg cccggtggga ggcgatgcag gggatgcgcg
     5821 tcggggtcgc gaccatggga cgcgccctcc gccgccccgg gctcacgcgc aacaaaagac
     5881 cctgatcgcc cgtgagcagg acccggtggc ccgcgactcg tggcagactc ggctgacccc
     5941 attgatgccc atcgactgat cgttctggac gaaaccagca cccccgtgac gctcacaccc
     6001 cggtgggcgc gcagcccccg gggtacccgg gtcaccggtc gcgtcccgcg tgggcggtgg
     6061 acggccgtga cgttgctggc cacgctcccc ccgacggact gggaccgggg ctgcacttcg
     6121 ccggggcgct ggaccggtcc accttcgaca cgttcgtggc ggatctgctc gttcccagcc
     6181 tgcgaccgag cgatatcgtc gtgctggaca acctgtcggt gcaccagcgt gcgatggctc
     6241 gtcagcggat cgaggcggtc ggggcgcagc tagggttcgg gcccacgtcc tcgcccgact
     6301 tcaatccgac cgagcaggcc tttgccaagc tcaaacacct gctgcgccag acagccgccc
     6361 gctcggtcga cgctgtcctg gcggcaaccc agcacctctc cccccgcatg acttgccaag
     6421 acacgcacgg ctcctatcgc cacgccgggt acaccttgtg atcaccgctc cagtggctca
     6481 ccaactaggt atgaagtgaa ctgctcaata ggactcgcag cctagtccgt cattgtcatt
     6541 tccatcgaaa ccatggggat cgctgccgat gacggtcacg ctgaatccga tatctccgca
     6601 gtcgaggtca taggagacga tcgggacgca cggcgtgtag ttcggatcac acccaccggc
     6661 tgctggaacc tctgcggccg gctcctggac gggaggtgga gcaggttctg ccggcacctg
     6721 gacggctgcc ggtggttgtt gctgcactgc cggtgcttgc gttggtgccg gcgcgcccgc
     6781 tgtcaaggga atatcgaagc caccacacaa cccccacacc ccgaggtcat gacggtcggc
     6841 aaatgccgcg gcatccttga actgctggtc gtacttgcgg tccccgtaat ccacgtcctc
     6901 cgcatagccg ttgttgatca ggacatggtt gagcatatag ggctggccac cgacctcgaa
     6961 ccagatatag cccaactcgc ggccataccg gtccttctca gtggtgtcct tctcgatgta
     7021 gacagtgccg gggttgtcgt tgaaggagag cacccattcg gcgaaggccg tggcctctga
     7081 cccaccacag tgctgctcgt tctgggtctc cggggtatcg gcgcggtaga tccgcacccg
     7141 gttggagacg ccgttgatca gcacctcgaa ggtatcgcca tcgacgatcc ggacgatctg
     7201 cgcctcctgc acatcctccg ggatcctgtc ggtccagaaa gccggggcaa agccgggagg
     7261 cagttccggg caggctttgc catcattgtc ggggtccagg gcgtcgtact tctgagggtc
     7321 agactcaaag acggattgcg cccattccca agcgtcgtag tcggtgcagg agactgactg
     7381 ggcagcggca ggagctacag cactcaacag g
//
LOCUS       DASYWM010000053        13819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_1795, whole genome shotgun sequence.
ACCESSION   DASYWM010000053 DASYWM010000000
VERSION     DASYWM010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13819
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..466)
                     /locus_tag="VGR29_02020"
     CDS             complement(128..466)
                     /locus_tag="VGR29_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2072395.1"
                     /translation="MLTAAAAVRGEITADLGEWFLQPAAGASVVRMSDVASEPIDWLW
                     ERWIPTRMLTILGGYGGDGKSTVMASLIGAWTTGSALPDGSLARRTMCRTRSGRGWTC
                     MERIRARCLC"
     gene            896..1264
                     /locus_tag="VGR29_02025"
     CDS             896..1264
                     /locus_tag="VGR29_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV2072396.1"
                     /translation="MQTYVALLRGINVGAHKRIAMADLRALIEGLGYADVRTHLNSGN
                     AVFASQVGVNQDVASTIRAAVASRPGMNTAVVVRSGEEMQRIIAGNPFPDRADDHKTL
                     HVAFLAGTPDRQLVEALAGI"
     gene            1286..1435
                     /locus_tag="VGR29_02030"
     CDS             1286..1435
                     /locus_tag="VGR29_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072397.1"
                     /translation="MIGDNVHLSYPNKLTGASLTPDMLEKTVKVATTLRNWRTVTALA
                     GMTVR"
     gene            1432..2124
                     /locus_tag="VGR29_02035"
     CDS             1432..2124
                     /locus_tag="VGR29_02035"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV2072398.1"
                     /translation="MTRTRAEGFPPMMIGDLEQGHRVNLPQWVRIYRVVLGALALYAV
                     YHNRFVQTYDEHFYQLFTNQSNVIAGIILILGGTLFARRHPPLWWDYVRGAGVMIMVT
                     TGAVYALLLGGLYNPFGDAHPLMDSVLHQLIPVVMVLDLLIVPLSPRVSWWGLLFFSI
                     YPLLYLGYSIANGARTDWYPYNFLNPGINGGVAGVAITVCLLMIGFLLLSALVISFSR
                     RASRPADQEPGP"
     gene            2581..3720
                     /locus_tag="VGR29_02040"
     CDS             2581..3720
                     /locus_tag="VGR29_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2072399.1"
                     /translation="MSPSSEGSVLNVGIVGSGGIARQHAIGWTAYPGKARIAAFADIA
                     IDRAKTMSGTHTDGVANAYASLDEMLADESIDVIDICLPHHLHTDAVIAAAKAGRAVL
                     CEKPLCTNLDDARKIKQALDESGVIFMSAHNQLFQPSLLEARRLISSGTIGTPYFFRS
                     IETFQARSFNPHSNEMAEVGAGQKGWRGDLAQSGGGELLDTGYHSTYRLLSLANNDRP
                     VEVTGFLSRFLIGHLPTEDTGQVMVRFESGAMGEILTSWALDVVGSVQFEASGEHGAL
                     AGSPTDLRHQIYRWPEPAQREFTEISSFTAEIGHFIDVVTNGADNPANIDIATRVLQL
                     IKGAYLSAKLGQTVTLPENPTDDPVAGSQGATTPFSVEVPDAVLS"
     gene            3746..4615
                     /locus_tag="VGR29_02045"
     CDS             3746..4615
                     /locus_tag="VGR29_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV2072400.1"
                     /translation="MFNALIPGVIGIRGLNLAESINLAKEAGFQGVSFDIVEAKTIAD
                     EHGVDHLRAMFADAGLRPAVWSPPVNWTSDETRDRQIAELGPYAELGAQLGATNVTTG
                     IMPANNDRPYDEQYEYLQTRLRPYAAALAGSGIRIGIEFITPKTLRDQFTYQFIYKMQ
                     DMLAFAKDIGTGNVGVLFDVWHHYCAHGTLEDMDVLTAEDVQLVHTNDAPAGITIDEQ
                     QDLSRTLPMETGVIPAPEMLRKLDEIGYDGAVAAEPFSQRINDLAARDPLAAARETCE
                     AQKKLFAAAGLSA"
     gene            4664..5161
                     /locus_tag="VGR29_02050"
     CDS             4664..5161
                     /locus_tag="VGR29_02050"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV2072401.1"
                     /translation="MHESQADLERIQQLLNESRRSMGEHMRSILSLERRLDARELVDR
                     LQGMRLLSLATVTAKGEPRVGPVDGLFYRGQFWFGSAPESVRFRHIRRRPSVSAVHLE
                     SEAFAVTVHGTATLIDVNAAMHRGVRDYCLEIYGESWHEWGAPAQYARIDADRMYTYC
                     VRDES"
     gene            5361..6197
                     /locus_tag="VGR29_02055"
     CDS             5361..6197
                     /locus_tag="VGR29_02055"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HEV2072402.1"
                     /translation="MVGEELDVSTKAGRQARWRARRPVQPAALVAPRFWEIDAARGIA
                     IGMMVVYHLVYDLDYVGGFDLNATRGFWRLFASGTAFLFLFLVGVSLAVSYDRSCQTA
                     ASEPRLFWKYASRGVRILGYGMLITLVIWLAVPDGQIYFGILHAIGLSIILAYPFLGR
                     PTLALLGAIPVLLAAPIVQTLRMDEPWLLWLGIRAPVGQMLDYRPMIPWFGVVLLGIV
                     AGSYLYRDGERRFPWPVQTPFSLGRPLVTLGRHSLAIYLLHQPLLLGVIYALSRVDLG
                     FF"
     gene            6401..6859
                     /locus_tag="VGR29_02060"
     CDS             6401..6859
                     /locus_tag="VGR29_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072403.1"
                     /translation="MATLSTDPETTVAVSSIFGADGPTVLIFPCTESSQRGVRVKGAA
                     IATWEAVDDSSGHFTAVQVIANVEGDYMGTLTIETFPSVDEASSSFEDNRPDICMMFR
                     DASNAAVAESPLPTPIRMLGYGMTPGDISFDSALDEQPPRTLPSRPGPRP"
     gene            7039..9639
                     /locus_tag="VGR29_02065"
     CDS             7039..9639
                     /locus_tag="VGR29_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2072404.1"
                     /translation="MCALSSAPFPNPLSAPGTTAGVPVTPSCRILGRDSEIGQILSLL
                     DRDDLRIVTLLGPGGVGKTRLAQEVVRILAPNFAHGARFVPLEAIRDPDLVPKAVAQL
                     LGLQESADLTAAELLNDMLADRHLLLVLDNMEQVVGAASPWLSDLISHAPRLRVLVTS
                     RIALNIACEQQFIVPPLPIPDGQVEESAAVTLFAERARAVHHAFELDDETTSIVAEIC
                     RQLDGLPLAIELAAARMKILSPQVLLARFTDRLTLLTGGHQDIPLRLRSMRDAIGWSY
                     DLLSRDEQRLFRRLSVFLGGFTLESAGFVARWEDSPETDPQGIDTLQSLVDQSLIQPM
                     PDAVDTRFRMLETIREYGLREVLKHGEVDEAYGAHADYVRNLAHHAEIALTGPDQAMW
                     LDRLEAEYPNIRAAMTWLETHGRLDEAIEINVQLQFFLEVRGHSIEMLERVESWLGLP
                     EIASRTRSRGMALLSAGGFLLKTDDSNRSITSLAEAADILQEVGDLRHAAVAHETLSA
                     IGMHVHMYEQMRIAAATALEIAEPLGLHRIVSTSLDHLSLYAGLTGDADLARKLMQQS
                     FDVAWAGGDLSVLAYHSCSHSDEAALQRDFDRAEHFAQEALRLMQALGSRVDLPNAWG
                     RLAWIALRRGDLELAAKNITTALSIAESCGNLLLVTHMMMRKGIIDAERGEFATAAEE
                     LQKALSYYERHRHVTDIAFCLDAYAFLATRAGDALPATRFLSASDALLQQVGVDQQIL
                     SDVVDLAGAFRQRGALRSMLGAAVFDRGYREGASLATHAAVAEALAYKPSSPQAEKKD
                     EAALGVDLSSREMDVLKLMTNGLSNQQIADTLFLSLRTVTTHVTKIFTKLGVQSRTAA
                     VSCAIRHGID"
     gene            complement(9707..11233)
                     /locus_tag="VGR29_02070"
     CDS             complement(9707..11233)
                     /locus_tag="VGR29_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEV2072405.1"
                     /translation="MSSNASSSPASPERRFQGGALRQRWPTLIVLPALIALLGAAAIS
                     PLIALFLGESFGISGGRDAPWPWAFAIIGLLAFWSAKVVGRLNLSLNASAALLFALGL
                     VTVALWWALEPTYDLGPVLRDPVSLVSGNGHLVVPMLIALGVWYQGLRYDFNPSLFAP
                     EEIRGNVQRSWAILAGSIVLAAIVGGDTGDAGIAAAGIAVPVAMICSAGAVAASEINN
                     TRSLARRRGSTAPGWDRWARFFAGIVVGIVLITGIAAILLGPGALDLVMEGLRTAFRA
                     FGVVLYWVMFGFIYALYWVYRFVAWILNSLFGDLLPEMQMPEVQQAPVAEHEIVQPEV
                     ESEEAPYATILRLVALGIALLVVAVIVFRLSRAGGSGDGEGEPDEERESVFSTSLARQ
                     QLRDLFRRKPKPERPRKLNLDRPPQSVREGMLYLQVLASRQRVPRSGTETPADFTTRL
                     ASEWPSVAAPLQVIRDRYEVVRYGETEQDPAAVVDAWRIIWQHRKDAPLPVEPEDART
                     "
     gene            complement(11196..12524)
                     /locus_tag="VGR29_02075"
     CDS             complement(11196..12524)
                     /locus_tag="VGR29_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV2072406.1"
                     /translation="MATSLPGKQGVEPGRPARVLAWWENREADINIRAWLGGAGALLL
                     LAAILREPILALVALGIALFGLTLRIWWDHALDKLSYTRHFAATRAFFGDEVTVELVA
                     VNAKPLPVSRLDVSEYVGPNVRIAGQELVRSAESRQMVLTTMYSLGMYERVSHRYTIV
                     CHRRGWHRLGPVKLVANDPFGMTARQMELEKQDGVLVYPRIVPMTDLVVPARQPLGDM
                     KPATPLVEDPMRMSGVRPYVPGDSPKRIHWRATARTGEMQTRVFEPSATPVAAIFLDT
                     ITFSHLWEGQNSDLLELSVTVAASMAAQLLEGRHQVGMYANAPIPNLARTVRISPGRR
                     PGQLTRILERLAMLAPAFGDRIEKMISEELSRLPWGASIVLVTSRVSPELQRTLLRLA
                     RSGGVQRFVLIAIGEQPELVPEIRRRVAVYHLGMEETWDVIQRIQLTRIS"
     gene            complement(12530..13504)
                     /locus_tag="VGR29_02080"
     CDS             complement(12530..13504)
                     /locus_tag="VGR29_02080"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV2072407.1"
                     /translation="MSDGGSASRLIRPVAERIIAEVEKVIVGKREVIEQLTVAILCEG
                     HVLLEDVPGLGKTMLARSLAGTLGGDFSRVQFTPDLLPSDITGSLVFDPRSAEFSYRE
                     GPIVTNVLLADEINRAGPRTQSALLEAMEERQISVERHRVRMPRPFLVVATQNPVELE
                     GTFPLPEAQLDRFLMRLSIGYPELEDERMMLRRFRTDTPLEAVETVTTTQEMVGLIAA
                     VRTVHVSEVVEDYLLRIIRATREHDALDVGASPRAALALARASQAYAAVQGRDFVKPD
                     DVRLMTEPVLAHRVIPTAQADLLGQSTQELIRSIASGIDVPVETANVA"
BASE COUNT         2648 a         4481 c         4119 g         2571 t
ORIGIN      
        1 aggtagctgc tcagcgggtc gatcaccacc aacccgatcc cgttcgccgc gatcacctcc
       61 ctcatcacct cgacatccct cctcaggtcg aaccaccgcg tccggccgtc cccacgaagc
      121 gtccccttca acacaagcac ctggctcgaa tccgttccat gcaggtccag ccgcggccgg
      181 atcgcgtacg acacatcgtt ctccgcgcaa gggagccgtc cggaagcgca ctgcccgtcg
      241 tccacgcccc gatcagcgac gccatcaccg tcgacttccc gtcgccacca tagccaccta
      301 ggatcgtcag catccgcgtc gggatccacc gctcccacag ccagtcgatg ggctccgagg
      361 ccacatccga catgcggacc accgatgccc cggccgctgg ctggagaaac cactcaccga
      421 gatccgccgt gatctcccct cgcaccgctg cggcagccgt gagcactgcc ccacccccgc
      481 atccttcccg cgtttcccta gcatcgccgc tcccttcgcg caccctttac cgtcccggga
      541 gaccggggga gaacaatcgc cctcaatagg aagacgtatc gggcagggca atctgcgcca
      601 ggcaactcgt cgcgatggtg cgggaatgcg gccctgcctc tcccgcaggc caatagcctg
      661 ccctgagtgc agtcaaaggg gtcgacccat cctcgcaccc accgatctta ggagcatatc
      721 ctgcgctgcc gccgctgcgt tgtgcggtgc cccacccatt tcctgatcgc agcccacttg
      781 cggatgacac gcacccagtc gccggccgat aattcacgcg tgaccgggta cgcggggaaa
      841 gggtgaaaga tttccatccc gcgccccact tgtctcatca catacagggc cggatatgca
      901 gacttacgtc gccttgctgc gcggaatcaa tgtcggggcc cacaagcgca tcgcgatggc
      961 cgacctgcgt gcgctgatcg aggggctcgg ctacgccgat gtgcgcaccc atctcaacag
     1021 cgggaatgcg gtcttcgcga gccaggtagg ggtgaaccag gatgttgcct ccacgatcag
     1081 ggcggcagtc gcctccaggc cagggatgaa caccgccgtg gtcgtgcgtt ccggcgagga
     1141 gatgcaacgc atcatcgcgg ggaacccctt cccggaccgt gccgacgacc acaagacgct
     1201 ccacgtcgcc tttctcgcgg gaacccctga tcggcaactt gtggaggcgc tggcagggat
     1261 ctagaaggga gaagacgact accgggtgat cggcgacaac gtccacctct cctaccccaa
     1321 caagctgact ggcgccagcc tcacacctga tatgttggag aagacggtca aagttgccac
     1381 aaccctgcgg aactggcgca ccgtcacggc acttgcaggg atgaccgtcc gttgaccagg
     1441 acgagagcag agggttttcc accgatgatg atcggcgatt tggaacaggg acaccgcgtg
     1501 aatcttccac aatgggtgcg catctaccgg gttgttctgg gtgcgctggc gctctatgcc
     1561 gtctatcaca accgcttcgt gcaaacgtac gacgagcact tctaccaact cttcacgaac
     1621 cagagcaacg tcatcgccgg catcatcctg attctcggag gcacgctctt cgcccggcgc
     1681 catccgccgc tctggtggga ctacgtccgc ggcgcgggcg taatgatcat ggtcaccacc
     1741 ggcgcggtct acgcgttgct cctcggaggt ctttacaatc ccttcggcga tgcccatccg
     1801 ttgatggact ccgtgctgca tcagttgatc cccgttgtca tggttctcga tctcctgatc
     1861 gtgcctctga gtccacgggt gagctggtgg ggattgctgt tcttctccat ctaccccctg
     1921 ctctaccttg ggtactcaat tgcgaacggg gcgcggaccg actggtatcc ctacaatttc
     1981 ctcaatccgg gaattaatgg cggtgttgcc ggagtggcga taaccgtctg cctgctcatg
     2041 atcggcttcc tcctgctctc tgctctggtg atctccttca gccggagggc ttctcgcccg
     2101 gctgaccagg aaccgggacc ctaagcggct tcgtcgagag gttggataca tgtagcggag
     2161 gacttgcggt gcatcctgcc ctgtccacgg gcatgaatgc aacaatgtcc tcctcgcagg
     2221 ccgatagtgc gacatagctg cggcaagtcg caggggtgag cccatgggcg aaaaaggctc
     2281 gctccgcttc tcggtggtgc tgccacctcg gccatgggct cgctccgctt ctcggcggct
     2341 gtgcggcagc ggcccctggc tgagcctgga aggtgcaagg cgaaaagcgg cagcgagtcc
     2401 ttcgctacac tcctcgctca gggatcggcg atatccatct cattgcccgg ggcgtcttcg
     2461 cggattggag aaccacttcc ccttgtgcaa atcctgtggg acgctacaat ccggcgcatt
     2521 acggtcaggg ggcatctggt ccccaccgct gaacaggtga gcacaaggag cgatctatcc
     2581 atgagccctt cttctgaagg aagcgtcctt aacgtcggta tcgtcggatc cggaggcatc
     2641 gcacgtcagc atgcgatcgg gtggaccgcc taccccggca aggcccgcat cgcggccttt
     2701 gccgatatcg caatagaccg cgccaagacg atgagcggca cccacacgga tggtgtcgcc
     2761 aatgcctacg cctcgctcga cgagatgctt gccgacgaga gcatcgatgt catcgatatc
     2821 tgcctgcccc atcatctcca caccgatgcc gtcatcgccg ccgccaaggc tggccgggcc
     2881 gtcctgtgcg agaagccgtt gtgcaccaac ctcgacgacg cccgcaagat caagcaggcg
     2941 ctcgatgaat ccggcgtgat cttcatgagc gcccataatc agctgtttca gccatccctg
     3001 ctggaggcac gccgcctcat ctcctccggc accatcggca cgccctattt cttccgctcc
     3061 atcgagacct tccaggctcg ctccttcaat ccgcatagca acgagatggc cgaggtcggg
     3121 gcaggccaga agggatggcg aggggatctt gcccagtcgg gcggcggtga gctgctggac
     3181 accggctatc acagcaccta tcgtctcctc tccctggcga acaacgatcg cccggtcgag
     3241 gtcaccggct tcctcagccg cttcctgatc ggtcacttgc cgaccgagga caccggtcag
     3301 gtcatggtcc ggttcgagtc gggcgcgatg ggggagatcc tgacgagctg ggctctcgat
     3361 gttgtcggga gcgtgcagtt cgaagccagc ggcgagcatg gtgccctcgc cggctcaccg
     3421 accgatctac gacatcagat ctacaggtgg ccggagccgg cacagcggga attcacggag
     3481 atctccagct tcaccgccga gatcggtcat ttcatcgatg tggtcaccaa cggagccgac
     3541 aaccccgcca atatcgacat cgcgacgcgc gtcctccagc tgatcaaggg agcctatctc
     3601 tcggccaagc tgggacagac cgtcaccctg ccggagaacc cgaccgacga ccccgtcgcc
     3661 ggatcacagg gcgcaacgac gccgttcagc gtcgaggtac cggacgccgt tctcagctaa
     3721 tcggtaaggg aggatattat ccatcatgtt caacgcactc attcccggcg tgatcgggat
     3781 ccgcggtctc aacctcgccg agtcaatcaa cctcgcgaag gaagccggct tccaaggcgt
     3841 ttcgttcgac atcgtggaag ccaagactat cgccgacgag cacggtgtcg accacctccg
     3901 agcgatgttc gccgacgcgg ggctcaggcc cgccgtctgg agcccaccgg tcaactggac
     3961 gagcgacgag acccgtgaca ggcagattgc cgagcttggc ccatacgccg aactcggcgc
     4021 ccagcttggt gcgacgaatg tcaccaccgg catcatgccc gccaacaacg atcgtcccta
     4081 cgacgagcag tacgagtacc tgcagacgcg tcttcgcccc tatgcggcgg ctctggccgg
     4141 gtcgggcatt cgcatcggca tcgagttcat taccccgaag acgttgcggg accagttcac
     4201 ctatcaattc atctacaaga tgcaggacat gctcgcgttc gcgaaggata tcggcacagg
     4261 gaatgtgggc gtgctcttcg acgtgtggca tcactactgc gcccacggca ccctcgagga
     4321 tatggacgtg ctgaccgcgg aggacgtgca actggtgcac accaacgacg caccggcagg
     4381 tatcacgatc gatgagcagc aggatctctc gcggaccctg ccgatggaga ccggggtgat
     4441 tcccgcgccg gagatgctgc ggaagctcga cgagatcggc tacgacggtg ccgtcgctgc
     4501 ggagcccttc agccagcgca tcaatgacct tgccgcccgg gacccgctcg ccgccgccag
     4561 ggaaacctgc gaggcccaga agaagctctt cgccgccgcc gggttgtcgg catagagggg
     4621 gaagtccccg gttgcgaaac gaaatccggt cagggatatc ccagtgcacg agtcacaggc
     4681 cgacctggag cgcatccagc aactgctcaa tgaaagccgt cgttcgatgg gggagcatat
     4741 gcgctctatc ctgtcactgg agcgacgcct ggacgccagg gagctcgtcg accggttgca
     4801 aggcatgcgg ttgctcagcc ttgcaaccgt gaccgccaaa ggcgagccca gggtcggccc
     4861 cgtcgatggg ctgttctacc gcggccagtt ctggttcgga tccgctcctg agtctgtccg
     4921 gttccgccat attcgccgcc gaccgtccgt cagcgcggtc catctcgaga gcgaagcgtt
     4981 cgcggtgacg gtccacggca ccgcgaccct gattgacgtc aatgccgcca tgcatcgggg
     5041 ggtcagggac tattgcctcg agatctacgg cgagagctgg cacgaatggg gcgctccggc
     5101 tcaatacgcc cgcatcgatg cggatcgcat gtacacctac tgcgtgcggg atgaatcctg
     5161 aacccagaat ccgatggccg cgaggttacg catcggaagc cggtcaccca ctcaacgtcc
     5221 cattcctcca cccacggcat gacagcagca cgaatctctg cgtggcgtgc tgtgcgaaaa
     5281 aatgtggagg ccaacaggtg tggactgctc gtgtaggctt tcctgaaccg gcctctcgat
     5341 gatacgggga cggagctcgc atggttggag aggaactcga cgtcagcaca aaagcaggga
     5401 ggcaggcacg atggcgcgct cgtcgcccag ttcagccggc tgcactcgtc gcgccacggt
     5461 tctgggagat cgatgccgcg cgcggcatcg ccatcgggat gatggtggtc taccacctcg
     5521 tctacgacct ggattacgtt ggtggcttcg atctcaatgc caccaggggc ttctggcgcc
     5581 tgtttgcctc aggcacggcg ttcctgttcc tcttcctggt gggcgtctcg ctcgcggtga
     5641 gctacgaccg ctcttgccaa acagccgcca gtgagcctcg tctcttctgg aagtacgcgt
     5701 cgcgcggcgt gcgcattctc ggctatggca tgctcatcac actggtgatc tggctcgccg
     5761 tgccggacgg ccagatctac ttcggcatcc tgcacgcgat cggcctttcg atcatcctgg
     5821 cctacccatt cctggggcga ccaacgcttg ccctgctggg cgccatcccg gtgttgctcg
     5881 ctgcaccgat cgtgcagacc ctgcgcatgg acgagccctg gttgctctgg ttaggcatcc
     5941 gcgcgccggt tgggcagatg ctcgactacc gcccaatgat cccctggttc ggtgtcgtgc
     6001 tgcttggcat cgtcgctggc agttatctct accgcgatgg agaacggcgc ttcccttggc
     6061 ccgtgcagac accgttttcg cttggccggc cactggtgac tctcggtcgc cactcactcg
     6121 ctatctacct gctgcaccaa cctcttctcc tcggtgtgat ctacgcactc agtcgcgtcg
     6181 acctcgggtt cttctagcga acccgacgcg cgaacccgat tactccatcg cctgattcgc
     6241 cctgtacaca tgcggtcggt tcgatcagcg accggaatgg aggtatcgag tacgggccgg
     6301 cactctcggt ctcgcgttcg catcgcagtt cgcaactgcc aatgcacggt cgcagacggt
     6361 ctcttttgca gtccatccac tgaccggcct ctggctgaac atggcaacgc tctcgaccga
     6421 tcccgaaaca acggtcgcgg tctcaagcat ctttggggct gatgggccca ctgtactcat
     6481 attcccctgc acggaaagta gccaacgtgg tgttcgggtg aagggagctg ccattgccac
     6541 gtgggaagcg gtggacgaca gttcgggtca cttcacggcc gtacaggtaa tcgccaacgt
     6601 cgagggcgac tatatgggaa cactgacgat cgagaccttt ccatcggttg acgaagcatc
     6661 gagctcattc gaagacaatc gaccggacat ctgcatgatg ttccgggatg cctctaatgc
     6721 ggcggttgcc gagagcccgc tgccgactcc aattcgcatg ctcggatacg ggatgacgcc
     6781 gggcgatatc agtttcgact ctgcacttga tgagcaacca ccgagaacct taccctcccg
     6841 gcccggccct cgaccctgat tcacccccaa ccgaggatca acgacataaa cgtccttcca
     6901 tggttaccac acgttatgcg aaatcctggg caaatgaaat tcctgctgtg tcgtctaaac
     6961 aggtcttgac gtaatccgtg cgcaggatca gaatgggtca gatacgatac cggaagagtc
     7021 cccgaggcag gagccttcat gtgcgccctt tcctccgccc catttccaaa cccgctatcc
     7081 gcgccgggta caacggccgg tgtgcccgta acgccgtcgt gccggatctt gggtcgcgat
     7141 agcgagatcg gccagatcct cagcttgttg gatcgggacg atctccgtat cgtgaccctg
     7201 ctcggtcccg gtggagtagg caagacgcgt ctcgcccagg aagtggtccg gatcctggct
     7261 ccaaactttg cccatggcgc ccggttcgtg ccgctggaag cgatccgcga tcccgatctg
     7321 gtgccgaaag cggtggccca gttgctgggt cttcaggaaa gcgctgacct cactgccgct
     7381 gaattgctga acgatatgct ggcggaccgc catttgctgc tcgtactgga caatatggaa
     7441 caagtcgtgg gagcggcatc gccctggctc tccgatttaa tatcccatgc tccccggctc
     7501 cgcgtgctgg tgacgagccg cattgcgctg aatatcgcct gcgaacaaca gttcatcgtt
     7561 cctcccttgc cgattccgga tggccaggtg gaagaaagtg cggctgtaac gctctttgcg
     7621 gaacgggcaa gggcagtgca ccatgccttt gaactggatg acgagacgac cagtatcgtg
     7681 gcagagatct gccggcaatt ggatgggctg ccgcttgcaa tcgagctcgc cgcggcccgg
     7741 atgaagatcc tctctcccca agtgctgctt gcccgcttca cggatcgcct gacactgctc
     7801 accggtggac atcaggatat tcccctacga ctgcgctcca tgcgggatgc gatcggctgg
     7861 agctacgacc tcctgtcgag ggatgagcag aggctctttc gccggctctc agtcttcctc
     7921 ggcggattca cgctcgagtc ggcggggttt gtggcaagat gggaagattc gcccgaaaca
     7981 gacccccagg ggatcgatac gctccagtcc cttgtcgatc agagcctcat tcaaccgatg
     8041 ccggacgcag tagacaccag gtttcgaatg ctggagacca tccgcgagta tggccttcgt
     8101 gaagtcctga agcatggcga ggtcgacgag gcatatggcg cgcatgccga ctatgttcgg
     8161 aacctggcac atcatgcgga gatcgcgctg acgggtcccg accaggcgat gtggctcgac
     8221 cggctggagg cagagtatcc gaacatccgt gcagcaatga cctggcttga aacccacggc
     8281 aggcttgacg aggccattga aatcaatgtc caactccaat tctttctgga agtccgcggt
     8341 cattcgatag agatgctcga acgtgtggag tcgtggctcg gtctcccgga gatcgccagc
     8401 cgaacacggt cgcgcggaat ggcgctgctc tcggcgggtg gcttcctgct taagactgac
     8461 gactccaacc gctccataac atcactagct gaggctgccg atattcttca ggaagtgggt
     8521 gatttgcggc acgccgccgt cgcccatgag acgctatcgg cgatcggcat gcacgtgcac
     8581 atgtacgaac agatgcggat cgccgcggcg acagccctgg aaattgccga accactggga
     8641 ctccatcgca tcgtcagtac tagcctggat cacctaagcc tgtatgcggg tctaacagga
     8701 gatgctgatc ttgctcgcaa gttgatgcag caatcgttcg acgtggcatg ggcgggcggg
     8761 gacttaagcg tactcgcgta ccactcctgc tctcattcgg atgaagcggc cctccaacgc
     8821 gacttcgaca gggcggagca cttcgcccag gaagcacttc gcctgatgca agcactgggt
     8881 tccagggttg acctgccgaa tgcttggggc aggctggcgt ggatagcgct acgacgcggc
     8941 gatctggagt tggcggccaa gaacatcacg accgctttgt cgatcgcgga atcctgtggc
     9001 aatctcctgt tagtgacgca catgatgatg cgtaagggca tcattgatgc agaacgcggt
     9061 gagtttgcga ctgcagctga agaacttcag aaagcattgt cgtattacga gcgtcatcgg
     9121 catgttacgg atatcgcatt ttgtttggac gcctatgcct tccttgccac tcgtgcgggc
     9181 gatgccctgc ctgctacgcg atttctcagc gcctccgatg cactgttgca gcaggtcggt
     9241 gtggatcagc agatcttgtc agacgtcgtg gacctggcag gagcctttcg acagcgaggg
     9301 gcgctgagat cgatgcttgg cgcagcggtg ttcgatcgtg gctacaggga gggagcgtcg
     9361 ctggcgaccc atgcggcggt agctgaagcg ctggcataca agccgtcgtc accccaggct
     9421 gagaagaagg acgaggcagc tctcggcgtc gatctgtctt cccgcgagat ggatgttctc
     9481 aagctgatga ccaacggact cagcaaccag cagattgccg acaccctgtt cctaagcctt
     9541 cgcacggtga cgacccatgt caccaaaatc ttcaccaagc tgggagtcca gtcacgcacg
     9601 gcagccgtgt cgtgtgccat tcgtcatggc atcgactgac acactcatgg ataaaggtgg
     9661 ggctaccagg gagtgccaac atgcgaattc acagcccgaa ggtcttctac gtccttgcat
     9721 cctccggctc cacagggagg ggggcgtcct tgcggtgctg ccagatgatg cgccaagcat
     9781 cgacgacggc ggcggggtcc tgctccgtct cgccatagcg aacaacctcg tagcggtccc
     9841 gaatcacctg cagtggcgcg gcaaccgacg gccattccga cgcaagacgc gtagtgaaat
     9901 ccgcgggggt ctcggtgccg gagcgtggca cccgctgcct gctcgccaag acctgcaggt
     9961 agagcatgcc ctcccggaca gattggggag gccggtcaag gttcagcttg cgtggccgct
    10021 ccggtttcgg cttgcgacga aagagatcgc gcaattgctg acgggcaagg ctcgtcgaga
    10081 acacgctctc gcgctcctcg tcgggctccc cttccccgtc gccggaacct cccgcacgag
    10141 acaaccggaa aacgatgact gcaacgacga gcagggcgat gccgagtgcc accagccgca
    10201 ggatcgtcgc gtatggcgcc tcttcagact ctacctccgg ctgaactatc tcgtgctctg
    10261 caactggcgc ctgctgcacc tccggcatct gcatctccgg caggagatcg ccgaagagac
    10321 tgttcaggat ccaggcaacg aaccggtaga cccagtacag cgcgtaaata aagccaaaca
    10381 tgacccagta gagaacaacc ccgaaggcac ggaatgcggt ccgcaagccc tccatgacga
    10441 gatcgagcgc ccccggtcca agcaggatcg ccgcgattcc tgttatcagc acgatgccga
    10501 cgacgatccc ggcgaagaac cgcgcccagc gatcccatcc gggagcggtg cttccccggc
    10561 ggcgcgccag cgaccgcgtg ttgttgattt ccgaggcagc gactgcaccg gcgctgcaga
    10621 tcatggccac agggaccgcg atgccggccg cggcaatgcc cgcatctccg gtgtcaccgc
    10681 cgactatcgc ggcgaggacg atcgaccccg cgagaatcgc ccacgaccgt tgcacattgc
    10741 cgcgaatctc ctccggggca aagaggctgg gattgaagtc gtagcgaagt ccctgatacc
    10801 agacacccag cgcgatcagc atcgggacga ccagatgacc gttcccgctt acaagcgaca
    10861 ccggatcgcg caacacaggc ccaagatcgt aggtcggttc cagggcccac cacagtgcga
    10921 cggtgacgag gcccagggca aacagcaacg cggcggaggc attcagcgag aggttcagcc
    10981 tgccgaccac ttttgcagac caaaacgcca gcaacccaat aatcgcgaag gcccatggcc
    11041 acggcgcatc acgccccccg gagatcccga aggactcccc gaggaagaga gcgatcaatg
    11101 gcgagatcgc cgccgcgcca aggagtgcta tcagcgcggg caggacaatg agtgttggcc
    11161 accgttgacg cagtgctccg ccctgaaatc gtcgctcagg agatgcgggt gagctggatg
    11221 cgttggatga catcccaggt ttcctccatc cccagatgat agaccgccac ccgccgccgg
    11281 atctccggga cgagctccgg ttgctcaccg atcgcgatca ggacgaatcg ctgcacgcca
    11341 ccggagcgtg cgagtcgcaa gagcgttcgc tgcagctcgg gactcacgcg gctggtaacg
    11401 aggacaattg aggctcccca tggcagccgg gagagttcct ccgagatcat cttctcgata
    11461 cggtcgccga acgcgggcgc caacatcgcc aggcgctcca gaatgcgggt cagctggccc
    11521 ggacgccgcc ctggcgagat ccggacggtc cgggcgaggt tcgggatcgg tgcgttggcg
    11581 tacatgccca cctgatgccg cccctcgagc agctgtgcgg ccattgaggc cgccacagtg
    11641 accgagagct caaggagatc ggagttctgg ccttcccaga ggtgggagaa ggtgatcgtg
    11701 tccaggaata tcgcggccac cggtgtcgca cttggttcaa agacgcgggt ctgcatctcc
    11761 cccgttcgcg ccgtcgcccg ccaatgaatg cgcttgggac tatcccccgg cacgtagggg
    11821 cgcacaccgg acatacgcat cgggtcctca acaagcgggg tcgccggttt catgtcgccc
    11881 aggggctggc gtgccgggac gacgagatca gtcatcggca cgatgcgggg atagacgagt
    11941 acgccatcct gcttctcgag ctccatctgc cgggcagtca tgccgaacgg gtcgttcgcc
    12001 accagcttca cgggcccaag gcggtgccac ccacgccgat gacagacgat ggtgtagcga
    12061 tggctgactc gctcatacat cccaaggctg tacatggtgg tcaggaccat ctggcggctc
    12121 tcggccgagc ggacgagttc ctgcccggcg atccggacgt tcggtcccac atattcgctc
    12181 acgtccaacc gcgagaccgg cagcggtttg gcattgacag cgaccaactc aacggtcact
    12241 tcgtccccaa agaacgcccg cgtcgccgcg aagtggcgcg tgtacgaaag cttgtccaga
    12301 gcatggtccc accagattcg cagggtaagc ccgaagagcg caatgcccag cgccacgagg
    12361 gcgagaattg gctccctcag aatcgcggcc agcagaagca acgcaccggc acccccgagc
    12421 caggcccgga tgttgatatc cgcctcgcgg ttttcccacc aggcaagcac tcgcgcgggc
    12481 cgcccgggct ctactccctg ctttccgggg agagacgtcg ccatcgagat caggccacgt
    12541 tcgcggtttc gacgggaaca tcaatcccgc ttgcaatcga ccggatgagt tcctgggtgg
    12601 actggccaag caggtcagcc tgcgcggtcg ggattacgcg atgcgccaac actggctctg
    12661 tcatcaggcg gacgtcgtct ggcttgacga agtcccggcc ctgcacggcc gcataggcct
    12721 gggacgcacg cgcaagcgcc agggccgctc gtgggcttgc accgacatcc aaggcatcat
    12781 gctcgcgagt cgcgcggatg atccggagca ggtaatcctc caccacctcg ctgacatgca
    12841 cggtacgaac ggcggcgatg agcccgacca tctcctgggt ggtggttact gtctcgacgg
    12901 cttccaatgg ggtatccgtc cggaagcgac gaagcatcat ccgctcatcc tccagctcgg
    12961 gatagccgat cgacaggcgc atcaggaagc gatcgagctg ggcctcggga agcgggaacg
    13021 ttccctccag ctcaaccgga ttctgggtgg cgaccaccag gaagggtcgg ggcatcctga
    13081 cccggtgccg ttccacagag atctgtcgct cctccatggc ctccagcaac gcggattggg
    13141 tgcgaggtcc ggcacggttg atctcgtccg cgagcaggac gttggtgacg atcggtccct
    13201 cgcggtagga gaactcggcg gaccggggat cgaagacaag cgagccggtg atatcgcttg
    13261 gcagcaggtc gggcgtgaac tggacccggc tgaagtcgcc ccccagcgtc cccgccaggg
    13321 aacgcgcgag catggtcttc ccaagacccg gcacgtcctc aagaagcaca tgcccctcgc
    13381 aaagaatcgc gaccgttagc tgctcgataa cctcgcgctt gccgacgatg accttctcga
    13441 cctcggcgat gatgcgctcg gccaccggcc tgatcaatcg cgatgcactt ccgccgtcac
    13501 tcatgcgcac ccctccagcg ttcccagtgt acgactacgt ccccgcccat ggagggcaat
    13561 tagaccacat catggcgcaa atgttgcgcg tatacttggg gtgtattcac tcgggagccg
    13621 ccttccgcga cgatgccggt ccttgctctc gcccccggat tccgcacagg aaaggacccc
    13681 ttcccctcgt ggtaggcctt cagttggcta tcattctgct gttgatcgtt gtcaacggtc
    13741 ttcttgccat gtacgagcta gcgatcgtct ccgcccggag ctcgcgcctc cagcaacgcg
    13801 ccgacaatgg cagcaaagg
//
LOCUS       DASYWM010000054         5441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_320671, whole genome shotgun sequence.
ACCESSION   DASYWM010000054 DASYWM010000000
VERSION     DASYWM010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320671"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="VGR29_02085"
     CDS             <1..1438
                     /locus_tag="VGR29_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2072408.1"
                     /translation="TPRWAPMEEELIGPNQELWLQRLDEEFPNIEFALQCGLDLDVEA
                     HNQGLILATSLWRYWTSRGRFASGTRLMRQMLDRPSTVGPTVRARALNNLGNLVFELG
                     QHVAAADYYEESRRLYETAGDINGVADELNNMGMIHVHNGDFEAAQDVMRQSMEIRKV
                     TGDSLALPTTLSNLGDMAIFQGDFDQAERYHREAYDLRCEIGNKRGIALSCYNLGTLA
                     ILRRDWDAAEIWHARGLKLSNEIGDAYSQACLHLGSGVVANHSGDVAKAVAFLTGALR
                     SFQDMETRRMLIETLDVIGLVAARHGHDTESARLLGASTELRRQYPLGSPARRAQWVH
                     DIVTGLRQRIGDQAWAAQTAIGTRWSIDQAVNAAFSVLQLIGTSQEQDRADVTDAGRM
                     IAPAGDDNAMTGVEDLTRREIQVLRLLAQGLSDKDIAERLSISPRTAMTHVSNILTKL
                     KVNRRSAASAVGLRAGLVPGRHAGDLEA"
     gene            complement(1477..2667)
                     /locus_tag="VGR29_02090"
     CDS             complement(1477..2667)
                     /locus_tag="VGR29_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV2072409.1"
                     /translation="MPFVPQSVFADRDKRIKEWLGANDMKALIVTTPDNFYMVSGFHL
                     DVAPWERPVVAVIPVDGKPFMVMNELSTNHLRFAEERKSLYITDYTIYVEHPRSVNRT
                     WTREQWGGLLAQRLAERGVTKGRVGVEGHGPSALKLAAPGLEFEDVTRKLIEMREVKY
                     PAELDIIRDCAALTDYGQDRYMELVKPGLNVKAFDFEISKLIMEEGARRFPKDRLEIR
                     MISLSGRYSASPHGTGAGAGETFEKGDGVVNIIVLRLSGLVVENERTLFVGKPRSDVQ
                     VRAFEAATEACEAAAAQMVAGNPVSSADAAAQQVYDKAGFGENINHRTGHGIGIAGHE
                     FPDDMPFLHRPFKEREVFSSEPGIYIWGVGGFRHDDTVIIGKKQPEIVTKRSKKLDDQ
                     IVPV"
     gene            complement(2686..3579)
                     /locus_tag="VGR29_02095"
     CDS             complement(2686..3579)
                     /locus_tag="VGR29_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072410.1"
                     /translation="MSTVPVAASGTSGIAVPRTRQNQLLRSLKRDPWASASLLFLLAV
                     VLAAILSPVLVPDTINSPSFGSRLLPPLTDGHIAGTDQLGRDVWGRMLLGARISMTVG
                     FLTVVLSALIGVPIGMLAGYYGGWLDVLLMRLVDVQLAFPFILLALTINAILGLGLRN
                     IIITLVIAGWIEYARIARGETLVVREREFVQAAHLTGARSGRIFLRHILPNIITPLIV
                     ISTLQVARFIIAEASISFLGFGVQPPTPAWGSMVSEGLDYIFSAWWLITLPGLALALV
                     ALAVNITGDWLRDTLDPRIRT"
     gene            complement(3576..4493)
                     /locus_tag="VGR29_02100"
     CDS             complement(3576..4493)
                     /locus_tag="VGR29_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072411.1"
                     /translation="MKGYVLGRLASGLIVIFLALTAVFFMLRLSGDPVLLFAPMDTSR
                     TNLEEIRERMGFNEPLAVQYATFMGDAIQGDFGESTRERRPAIEIVLERLPATLQLGG
                     LALVISLSVGIPLGILSATHHGSGWDKVARLIAVLGQAIPGFWLGVLLMLLFAVQLNW
                     LPTSGRGGVQHIILPAITLSALSTARYARLTRSTMLDVLGQDYMRTAKAKGLSNGTVL
                     WRHALKNASISLITMTGLEIGRLIEGAVVIEQVFAWPGMGRVTVQALLNRDFAVVMAA
                     VVLFAAMYTLANLVADLAYGWADPQVKLG"
     gene            complement(4527..>5441)
                     /locus_tag="VGR29_02105"
     CDS             complement(4527..>5441)
                     /locus_tag="VGR29_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072412.1"
                     /translation="LERNEEYWRNDVMVQEVEFRFSPEFSTRLAALLAGELGVVKDIP
                     VDSMQTVEESGTARIEAIPSSRINYVALVNNREDSVFNDVRLRQAVNYAVDVNGIIEG
                     IFQGEATRMAGPLSGLNPDVNPNLQPYEYDPEKARALIEEAGYAPGELEITMDAPQGR
                     YPMDTDAALAIAASLGDVGITVNVQYNEWGTHLDKIVNRQTGDMFYLGWGPALDAMGT
                     LAYLFVGDSTYSSYQNPEVEPLIEEATMTVDPEKRKELWFQVQQMVYEDAGWLFLWQQ
                     HDIYGVANDINWAPRADEFLWMGETEAR"
BASE COUNT         1079 a         1624 c         1690 g         1048 t
ORIGIN      
        1 tacaccgaga tgggcgccga tggaggagga gctgatcggc ccaaatcagg agctttggct
       61 gcagcgcctg gacgaggagt ttcccaacat cgagtttgca ctgcaatgcg gcctcgatct
      121 ggacgtcgag gcgcacaacc agggccttat cctcgcgaca tcgttgtgga gatactggac
      181 gagccggggc cgatttgcat ccggaacccg attgatgcgc cagatgctgg atcgaccgtc
      241 gaccgtgggg ccgacggtac gtgcccgggc gttgaacaac ctcggcaacc tcgttttcga
      301 attggggcag catgtcgctg ccgccgacta ttacgaggag agcaggcggc tatatgagac
      361 tgccggggac atcaacggtg tcgctgacga gcttaacaat atgggaatga ttcacgtcca
      421 caacggagac ttcgaggctg cccaggacgt gatgcgccag tcaatggaaa tccggaaagt
      481 gaccggagac tcactggcgc ttccgacaac gttgagcaat ctgggcgata tggcgatctt
      541 ccagggtgat ttcgatcagg ccgagcgcta ccatcgggaa gcctacgacc tccgctgcga
      601 gatcgggaac aagcggggta tcgcactctc ttgctacaac ctcggcaccc tcgcgatcct
      661 gcgcagggat tgggatgccg ccgagatttg gcatgcgaga gggctgaagt tgtcgaatga
      721 gattggcgat gcttacagtc aggcgtgcct gcacttgggc agcggagtgg tggcaaacca
      781 cagtggtgac gtcgcgaagg ccgttgcctt cttgactggg gcgctgcgat ccttccagga
      841 catggaaacc cgacgaatgc tgatcgagac gctggacgtg attgggctcg ttgcggcaag
      901 gcacggtcac gatactgagt cggctcgtct gctcggtgcg tcaacggagc ttcgacgcca
      961 atatcctctc ggttcgcctg ctcggcgggc ccagtgggta cacgatatcg ttactggtct
     1021 ccgtcagcga attggcgacc aggcttgggc tgcccagacc gcgattggca ctcgatggtc
     1081 gattgaccag gcggtcaatg ccgcattttc tgtgcttcag ctcatcggga cgtcacagga
     1141 gcaggatcgc gcggacgtga cagatgcagg tcgcatgatc gcgcccgcag gggacgataa
     1201 cgcaatgacc ggcgtggagg acctcacgag gcgtgagatc caggtgttgc ggctgctcgc
     1261 acagggactg agcgacaagg acatcgcgga gcgactctcg atcagcccgc gtacggcgat
     1321 gacgcacgtt tccaacatcc tgacgaagct caaggtcaac cggcgctccg ccgcgtccgc
     1381 ggtgggtctc cgtgccgggc tggtcccggg ccggcatgcc ggcgatctgg aggcataagc
     1441 gcggcgccga tcggctctgc gcttctctgt tccgggctag accggtacga tctggtcgtc
     1501 cagcttcttc gaccgcttgg tgacgatctc tggctgcttc ttgccaataa tcaccgtgtc
     1561 gtcgtggcgg aagccgccga caccccagat gtagatgcca ggctcggagg agaagacttc
     1621 ccgctccttg aagggccgat gaaggaaagg catgtcgtcc gggaactcgt gcccggcgat
     1681 gcctattccg tgaccggtgc ggtgattgat gttttccccg aaccccgcct tatcgtacac
     1741 ctgctgggca gccgcatcgg cgctggagac agggttgccg gccaccatct gggctgccgc
     1801 tgcctcgcat gcctcagttg ctgcctcaaa ggcgcgcacc tgcacatcgc tccgcggctt
     1861 gccaacgaaa agggtgcgct cgttctcgac gaccaggcca ctcagtcgca atacgatgat
     1921 attgacgact ccgtcaccct tctcgaatgt ctcacccgcg ccggcgccgg tgccgtgcgg
     1981 cgaggcggag tagcgtccgc tcaaggagat catgcggatc tcgagtcggt ccttcgggaa
     2041 gcgtcgtgcg ccttcctcca taattaattt gctgatctcg aaatcgaacg ccttcacgtt
     2101 cagccccggc ttgaccagct ccatgtagcg atcctggcca tagtcggtca gtgccgcgca
     2161 atcgcggatg atgtcgagct cggccgggta cttgacctcc cgcatctcga tcagcttgcg
     2221 tgtgacgtcc tcgaactcga gccctggtgc cgccaatttc agggcagaag ggccgtgccc
     2281 ctcgacgccg acccggcctt tcgtgacacc tcgctccgcc aggcgctggg ccagcagtcc
     2341 gccccattgc tcgcgggtcc acgtccggtt gaccgagcgc gggtgctcca cataaatggt
     2401 gtagtccgtg atatagagcg acttacgctc ctccgcgaaa cggagatgat tggtcgaaag
     2461 ctcgttcatt accatgaagg gcttgccatc gacgggaatc acggccacca ccggacgctc
     2521 ccatggcgcg acatcaaggt ggaatccgga aaccatgtag aagttgtccg gcgtggtcac
     2581 gatcagcgcc ttcatgtcgt tggcgccaag ccactccttt atgcgtttat cgcgatcagc
     2641 gaagacagac tgcggaacga acggcatctt gttgccctcc ttgggctacg tgcgaatacg
     2701 cggatccagg gtgtcccgaa gccagtcgcc agtgatattg acggcgaggg cgacgagcgc
     2761 aagcgcgagt cccgggagcg tgatcagcca ccaggcagag aagatgtagt ccaggccctc
     2821 gctgaccatg cttccccagg caggtgtcgg aggctgcacg ccgaaaccga ggaagctgat
     2881 actggcctcg gcaatgatga aacgggcaac ctgcagggta ctgatgacga tcagcggggt
     2941 gatgatattt ggcaggatat gccgcaggaa gatacgaccg cttctcgcgc cggtgaggtg
     3001 cgcagcctgg acaaactccc gctcacgaac gacgagcgtt tcaccccggg cgatacgggc
     3061 atattcgatc cagccggcga tcaccagcgt gatgatgatg ttccgcagac ccagaccgag
     3121 aatggcattg attgtgagcg caagcaggat gaacgggaat gcgagctgga catcaaccag
     3181 ccgcatcagc aggacgtcca gccagcctcc gtagtacccg gccagcatcc cgatcggtac
     3241 tccgatgagc gcggagagaa cgacggtcag gaatccgacc gtcatggaga tacgggcgcc
     3301 aagaagcatc cggccccaga cgtctcgtcc caactggtcg gttcccgcaa tatgcccgtc
     3361 ggtcagaggt ggcaggaggc gggaaccaaa gcttggactg ttgatggtgt ccgggacgag
     3421 taccggcgag aggattgcgg caaggactac ggcgagcagg aagaggagag aagcgcttgc
     3481 ccagggatct cgcttcagcg atcgcagcag ttggttctgt ctcgttcggg gaacggcgat
     3541 accggatgtg ccactggccg cgacgggcac ggtgctcatc cgagtttcac ttgcggatcc
     3601 gcccagccat aggccagatc ggccaccagg tttgccaggg tatacatcgc cgcaaagagg
     3661 acgacagccg ccatgaccac ggcaaagtcc ctgttcagga gcgcctggac ggtgacgcgg
     3721 cccatcccgg gccacgcgaa cacctgctcg atcaccaccg cgccctcaat gaggcggccg
     3781 atctccagcc ctgtcatggt aatgagcgag atactggcgt tcttcagcgc atgccgccaa
     3841 agcaccgtcc cgttggacaa tccctttgct ttggcagtcc gcatgtagtc ctgccccagc
     3901 acgtccagca tggtcgatcg cgtgagacgc gcgtatcgcg cggtactcaa cgccgacagg
     3961 gtaatggcgg ggagaatgat gtgctgtaca cctccgcggc cacttgtcgg gagccagttc
     4021 agttgcaccg caaagagcag catgaggagg acaccgagcc agaaccccgg gatggcctgc
     4081 ccaagcacgg cgatgagtcg cgcgaccttg tcccatcctg acccatgatg cgtggcggag
     4141 aggatgccga gcgggatgcc cacactgagt gagatcacca gtgccaatcc cccaagctgg
     4201 agcgttgccg gcaaccgctc aaggacgatc tcgattgctg gacgtcgctc gcgggtcgac
     4261 tcgccgaaat ctccctggat agcgtctccc atgaacgtcg cgtattgaac ggcgagaggt
     4321 tcgttgaacc ccatccgctc gcgaatttcc tccaggttgg ttcttgacgt gtccatcggt
     4381 gcaaagagga gcaccgggtc acctgacagc cgtagcataa agaagacggc tgtcagggcc
     4441 aggaagatca cgatcagacc ggaggcgaga cgcccgagga cgtagccttt cattcagtct
     4501 tccctaggtg gtgacgaaga acggaactat ctcgcctcgg tttcgcccat ccagaggaac
     4561 tcgtccgcgc gaggggccca attgatatcg ttggcgacac cgtagatatc atgctgctgc
     4621 cagaggaaga gccagccggc gtcttcgtag accatttgtt gcacctggaa ccagagttcc
     4681 tttcgcttct cgggatcgac ggtcattgtc gcttcctcga tcagcggttc aacctccggg
     4741 ttttgatagc tgctgtaggt ggagtcgccg acaaagagat aggcaagcgt gcccatcgcg
     4801 tcgagcgcag gtccccagcc aaggtagaac atatcgcccg tttggcgatt gacgatcttg
     4861 tccagatgcg ttccccactc gttgtactgg acattgactg tgatcccaac gtctccgagc
     4921 gaggcggcga tggcgagtgc cgcgtcggtg tccattggat aacgcccttg gggagcgtcc
     4981 atggtgattt cgagttcacc gggcgcatag ccggcctcct cgatcagggc gcgggccttt
     5041 tccgggtcat attcataggg ctgaaggttg ggattgacat caggattcag tcccgatagt
     5101 ggtccggcca tcctggtagc ctcgccctgg aatatcccct cgatgatccc gttgacatcg
     5161 acggcgtagt tcaccgcttg gcggagacgc acgtcgttga agacactgtc ctcgcggttg
     5221 ttcacgagcg cgacatagtt gatgcgggaa gatgggatgg cctcgatgcg ggctgttccg
     5281 ctttcctcga ccgtttgcat cgaatccacc gggatgtctt tcacgacacc cagctcacca
     5341 gcgagcagtg ccgcgaggcg cgtgctgaac tccgggctga agcggaactc cacttcctgc
     5401 accatgacgt cgttgcgcca gtactcctca ttgcgttcga g
//
LOCUS       DASYWM010000055         9929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_358213, whole genome shotgun sequence.
ACCESSION   DASYWM010000055 DASYWM010000000
VERSION     DASYWM010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358213"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VGR29_02110"
     CDS             complement(<1..341)
                     /locus_tag="VGR29_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072413.1"
                     /translation="MSMPKGPRNLRPHQGEYEDPLDNPTRDGVQQRFLDRLSIDRAQA
                     LVDLAGRPLELYHQMWQASNDAGALTPGVGPLVDLRLGKLPWREQFKYADEGEPWIPP
                     VYVDMHVALKH"
     gene            complement(646..717)
                     /locus_tag="VGR29_02115"
     tRNA            complement(646..717)
                     /locus_tag="VGR29_02115"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Lys,seq:ttt)
     gene            830..1672
                     /locus_tag="VGR29_02120"
     CDS             830..1672
                     /locus_tag="VGR29_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HEV2072414.1"
                     /translation="MVSTFSLSLGSDPVRWLLALVGALLITGTAYRLRSLDTSGVIAA
                     VLLGTIVVGAGGWWAGLLLITFFVSSSLLSRIGHNPTAVHATRGSRRDATQVFANGGI
                     ALLSAVCFWLFSHPAWLLAFAGSLAAANADTWSTEIGRTSRDLPRLITTGRRVSRGTS
                     GAVSTRGLVAAAAGASLIGALGGLGAAAGWFPVQHSWLFTVAAVGISGLAGSIADSIL
                     GATVQEQRWCDACQTQTERSIHQCGTPTHHLSAVWWISNDVVNLACVFVGGALACAAG
                     WIAS"
     gene            1896..3470
                     /locus_tag="VGR29_02125"
     CDS             1896..3470
                     /locus_tag="VGR29_02125"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2072415.1"
                     /translation="MRHPAFFQHTDRSIVRAGRAVVFAALLGTSAMLSPVVTAQDATP
                     STTPVASPVASPVVQADVQSTNLMTSQFDEFIPAPMTVRLLRITLEPGASAPMHTHSG
                     PEFDLIESGNLTVRSDGEAEVTRANGDTEVSADQDLVLGAGDWIVYEPGTGMSYENAG
                     EEPVVILSAVLLPVGSEFPESITYTDGQPTSADFEGVSFQVLGDGLVQDMPSGSATVS
                     IDSLIVPPGTDLPAAGTVAMYSLVEGNFSFIVESGPVQVSRSSLQSLQPNAVIGEEFI
                     LEPGDAAFFPAGVTATERRDETEPLELLTLTVDFEEELDEEPADLTFTNATGATGDAT
                     GGAEVAESVDEGSDGTDGEASGQIVTTNTDNLNLRAEPTTTADVVDQLAAGVELEVIG
                     GPVEAEDYTWYQVQVTAPGGNSGWVVAEFLDGLDAEPAAEPTEAPAANGTPAAAASET
                     FSVGDIVVTTDDNVRVRAGASLDADIIEAYPTGTEFEITGEPVEAEEYVWYPVTLVDD
                     PDISGWTTEDFIAPIE"
     gene            complement(3562..4380)
                     /gene="aroE"
                     /locus_tag="VGR29_02130"
     CDS             complement(3562..4380)
                     /gene="aroE"
                     /locus_tag="VGR29_02130"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV2072416.1"
                     /translation="MKRVGVIGDPVEHSLSPHMHNAAFGELGIDAFYELWPTPLPGLG
                     DRVASLRLPDVLGANVTVPHKQAVMPFLDRVTETAQRIGAVNTVIPSPEGLIGDNTDA
                     HGFQRSIEETFGPVQIDSAVVLGAGGASRAAIVALRMMGVENIDIANRTDARAAALAQ
                     EFAAEMIPWSEVVAESFPDAELLINATSLGWHDEMPVLMDDLKRLPHRAIVVDLTYRE
                     TPLLRAAADCGRRGLDGLGMLVHQGARAFELWTGMQAPVGIMQESVRAEQARRG"
     gene            complement(4416..5582)
                     /gene="mltG"
                     /locus_tag="VGR29_02135"
     CDS             complement(4416..5582)
                     /gene="mltG"
                     /locus_tag="VGR29_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV2072417.1"
                     /translation="MLSSALKVLSIVVLSVVVAGGSVWFFNYWQDRARSEEIGRPVTL
                     EVTQEDDTGTVAEKLTDAELVQYRSYFETRMRFSGVELRPGVYTLRMGMSVPDIIDVI
                     TVADTSSPDENTEAAAEITQSFQVTFIEGQRIEEYARVLQEAGMLDSAAEYIATANDV
                     EGFRGNYSFLESVPSGGSLEGFLFPDTYTVGENATPADVIGLQLSNFEAQFTPEMIAQ
                     AEARGMSIYEVVTLASIVEREAAIPEERPQIAAVYLNRLEAGMPLQADPTLQYVVGTE
                     QEWWPVLDTALLEQAKGSEFNTYDLQGLPPAPIANPGFASLQAVLQPAEVDYLYFVTA
                     GDDTGRHVFSTTYEEHLQNTCIEHPEYEECGGSGQRPTASPFAGFTSWAREVAA"
     gene            5695..6696
                     /gene="miaA"
                     /locus_tag="VGR29_02140"
     CDS             5695..6696
                     /gene="miaA"
                     /locus_tag="VGR29_02140"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV2072418.1"
                     /translation="MPVPFYVHASMTDQRDLHPDRAESGKPPLIVIVGPTAVGKTRLS
                     LMLAEQFNGEIVNADSRSFYRGMDIGTAKVSLEERERVPHHLIDILDPNDSVSLALFQ
                     DMAYASVRDITVRGKTSFLVGGTPQYVNAVVEGWNIPRVEPDSDFRLLLEREAAEHGF
                     ENLLERLRVVDPESAIRTGSNVRRIIRALEVYEKTGRPMSEQQSKGPAPFRALELGLW
                     RPREDLHRRIDERVHSQIAEGLFDEIRSLLSQGVSPESAAFTSIGYRQGMPYIRGEAT
                     ADEVAERIRLDTRRLVRHQETWWRKNPRLLRIDLTQPDETTRVQRAVAVHLGDEGGS"
     gene            6816..7709
                     /locus_tag="VGR29_02145"
     CDS             6816..7709
                     /locus_tag="VGR29_02145"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEV2072419.1"
                     /translation="MVTNFGHNIDSAFTGYAHPEALVTTDWVANHSNDPKVRILESDE
                     DIMLYEIGHIPGAVKLDWETEMQQQVNRDFVDRAGFERLCSERGITPDMTVVFYGDKN
                     NWYATYGFWLFKYFGHDDARVMDGGRTKWEAEQRPYTRETPKIEPAFYTAKDPDSSIR
                     AFRDEVLKHVETGAGPLVDVRSNPEYTGELLHAIGYPQEGAQRAGHIKGARNVPWGTA
                     ANADGTFKSAEELRELYGKQGVTGDKPVIAYCRIGERSSHTWFVLKYLLGYDDVRNYD
                     GSWTEWGSLVGVPNAVGNEPG"
     gene            7730..8170
                     /locus_tag="VGR29_02150"
     CDS             7730..8170
                     /locus_tag="VGR29_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEV2072420.1"
                     /translation="MLDRQDYIEIILDHYEQPRNKHRIENASVQLGGGNPGCGDLITV
                     YLDIGEDDRIREASFEGEGCTISQAGGSIIAELIEGMTLEEVKALGKETMIEEIGEDA
                     VKSRVRCATLALGTAQAAVDAYIRDRRRQHQGLDTLPEPEQFQN"
     gene            complement(8556..>9929)
                     /locus_tag="VGR29_02155"
     CDS             complement(8556..>9929)
                     /locus_tag="VGR29_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV2072421.1"
                     /translation="QRMATGCRARAKEQGKLLPGGHPLYGYQWDDPSKSAKTRHVEDE
                     AVSWIVRRVFEEAASGVPLRRIAMRLNDDGMTTTSGKRWQARQISRMLANPAYKGQVY
                     ANRYRVEKDEHTSRKRESIRPESEWVLIKDAAPPLISAETWQRANERLAKNRIEHSRP
                     YDKAEDALLRAGFIYCAYCGNRMNVSRRRHKIFYKCNSRYWGGKGECKGWSVKAGPVD
                     QFVWGRVEGWLGDPGVLFHEAERSRELVLNSVQEDLGAFDRGLAKLESEQDNLVQALT
                     RLSGSAAHPVVEKLNALATRRDALVTQREEALRRSELAAKAEKAMGVFWDTVRAYGDG
                     VGTWGYAEKRELLHRLGVRLTVEKRGRAQGKLLLEANPPWPEGTEWWMELFNDSYHIE
                     AEIGPGGDEDGDGEGGVTVLPSSDQISGKWRSIANRMSSLMDRMATGTRLPSARRVSP
                     KSSIVTM"
BASE COUNT         1933 a         3133 c         2928 g         1935 t
ORIGIN      
        1 cagtgcttga gggccacatg catatcgacg tacacaggag gtatccaggg ctctccctcg
       61 tcggcatact tgaactgttc ccgccatgga agcttcccca atcggagatc aacgagcggt
      121 ccgacgccag gcgtaagggc cccagcgtcg ttactggcct gccacatctg gtgatagagc
      181 tcaagtggac ggccggcgag atcaacgagg gcctgggccc gatcgatgct gaggcggtca
      241 aggaagcgtt gctgcacccc atccctcgtt gggttgtcga gcggatcttc atattcacct
      301 tggtgcggtc ggagattgcg aggacccttg ggcatactca tgttgtgctt acccctcctc
      361 agtagttgcc atcatcccgg ccggttcgct cccggcgcgg ggtgcttcat tggtcaaaga
      421 catcgttcac agttcacaga gctttaagta gatatgactg tgaatgaaga atatctgtga
      481 acgctgcctg caatggaggg gcacgcgttg gggcagcaca actacaccat tcgggtagtc
      541 acgaaatggt tttgtcgtca atgttgtcgt ctttaggtga acgagcatca agcgctcgaa
      601 acagcgttcg tacaattacc ccattgtcat ggggattttt gagggtgacc cccccgggat
      661 tcgaacccgg aaccaatgga ttaaaagtcc actgctctgc cgttgagcta gagggtcgcg
      721 ccgcacttcc gcgtgcgcca attcatccta ccgcgatgat gccgccccat gccatagtgt
      781 gcccagcggc aatacggtca ccggctccag caaggcatcg tgcataacca tggtctcgac
      841 cttctctttg tccctaggat cggatcctgt acgctggctc cttgcgctcg ttggcgcgct
      901 cctcatcacc ggcaccgcct atcgcctgcg atcccttgac accagtggcg tgatcgctgc
      961 cgtcctgctc ggtacgatcg tcgtcggtgc gggaggatgg tgggctggct tgctcctgat
     1021 cacctttttt gtctcttcca gcctgctctc ccgcattggc cataatccca ctgccgtcca
     1081 tgccacccgt gggagccggc gcgatgccac ccaggtcttc gccaacggcg gcattgcgct
     1141 cctcagtgcc gtttgtttct ggctcttctc ccacccggcc tggctgctcg cattcgctgg
     1201 ctcacttgcg gcagcgaatg cggatacttg gagtaccgag atcggtcgta caagccgcga
     1261 tcttccccgc ttgatcacca ctggcaggcg agtctctcgt ggaacgtctg gagcggtgag
     1321 tacaaggggc cttgtcgcgg ccgctgcggg cgcctcgctc atcggagcgc tcggcggact
     1381 cggcgccgca gccggctggt tccccgtcca gcattcctgg ctgttcaccg ttgccgcggt
     1441 gggcatctcc ggacttgccg gatccatcgc tgattcaatt ctcggtgcaa ctgtccagga
     1501 gcaacgatgg tgcgatgcat gccaaacgca aaccgagcgc agcattcacc aatgtgggac
     1561 gcccacccac catctttcgg cagtatggtg gatctcaaac gacgtggtga acctcgcctg
     1621 tgttttcgtc ggtggtgcac tggcgtgtgc agccggttgg atcgcctcat gatccggctg
     1681 aagcaacacc gtcaaacggt tggatggggt aagatcatga caagcgccac gatcagattt
     1741 cctgtgtctc gagcgtgacg ctggagcgtt ccaacaatgg ttacttccga actatcgccc
     1801 tctggtcgat gggaaacgat gccgatgtcc cgcgcgtcac cattactgag cgatttaatc
     1861 ccgcaaagtc aagcccggat atgatgagga gcaccatgcg gcacccagca ttcttccaac
     1921 acactgaccg gtctatcgtt cgtgccggcc gcgccgtggt cttcgcggcc ctgctcggca
     1981 ccagcgctat gctgtcccct gttgtcactg cgcaggatgc aactccctcg acaacgccgg
     2041 tcgccagtcc cgtggcgagc ccggtcgtcc aggcggacgt gcaatccacg aaccttatga
     2101 cgtcacagtt tgacgagttt atccccgccc ccatgaccgt gcgcctgctt cgcatcacgc
     2161 tggagcctgg tgcctctgct cccatgcaca cgcattcagg gccggagttt gacctcatcg
     2221 agtcgggaaa cctgacggtt cgaagtgacg gcgaagccga ggtgacgcga gccaacggcg
     2281 atacggaggt ctccgcagac caggatctgg tactcggtgc aggggattgg attgtctacg
     2341 agcccggaac cggcatgtcg tacgagaacg ctggcgagga accagtcgtc atcctgtcgg
     2401 cggtgctgct gcccgtggga tccgagttcc ctgaaagcat tacctataca gatggccagc
     2461 caacctcagc agatttcgag ggcgtctcgt tccaggtgct gggcgacggc ctcgtgcagg
     2521 acatgccgtc cggcagcgcg acggtctcca tcgactccct catcgtgccg cccggcaccg
     2581 acctgccagc ggcggggacc gttgcaatgt actcgctggt cgaagggaac ttctctttca
     2641 tcgtcgagag cggaccggtg caggtcagcc gcagcagtct ccagtcgctt cagccaaacg
     2701 cggtgattgg agaggagttt atcctggagc caggcgatgc cgccttcttc cctgccggcg
     2761 tgacagctac cgagcgtcgc gacgagaccg agccacttga gctgctcacg ctgacagtcg
     2821 acttcgagga agaactggac gaagaaccgg ccgacctgac gtttaccaac gcgacgggtg
     2881 cgaccggtga tgcgaccgga ggcgccgaag ttgccgaatc ggtcgatgag ggatctgatg
     2941 gtaccgacgg cgaggcatcc ggccagatcg tgacgaccaa caccgacaac ctgaacctgc
     3001 gagccgagcc gaccacgacg gccgatgtcg tggatcaact ggcagcgggc gtcgagctgg
     3061 aggtcattgg cggaccggtg gaggccgagg actacacctg gtaccaggtc caggtcaccg
     3121 ctccgggtgg caacagtgga tgggtcgtcg cagaattcct cgatggcctc gacgccgaac
     3181 ctgcagcaga accgacggag gcgccggcag ctaacggcac gccggcggcc gcagcttcgg
     3241 agacattctc ggtgggcgac attgtggtta ccaccgatga caatgtgcgc gtccgggcgg
     3301 gggccagcct ggacgccgac atcatcgagg cttatcccac gggcactgag ttcgagatca
     3361 ccggggagcc ggtcgaagcc gaggaatatg tctggtatcc ggttaccctc gtggacgatc
     3421 cggacatctc tggctggacc acagaggact tcatcgcccc gatagagtaa gccggggggg
     3481 aaagatcagt tgaagaaggg cccggccgca acagatgcag ccgggccctt ccttgccgat
     3541 gcctgggaat atggctaccg gttaaccccg gcgggcctgt tcggctcgta ccgattcttg
     3601 catgatccca accggcgcct gcatccccgt ccagagttca aacgccctcg ccccctgatg
     3661 aaccagcatg ccgagaccgt caagtcctcg gcgtccgcaa tcagctgccg cccggagcaa
     3721 cggcgtttcc cggtaggtca ggtccacgac gatcgctcta tgtgggagac gcttcaggtc
     3781 atccatgaga acgggcatct cgtcatgcca accgagcgat gtagcgttga ttaacagctc
     3841 cgcatccggg aatgattctg cgacgacttc actccaggga atcatctcgg cagcaaactc
     3901 ctgggccagg gcagcggccc gggcatcggt ccggttggcg atgtcgatgt tctccacacc
     3961 catcatcctc agggccacga tcgccgcccg ggaggcgcct cccgcaccga gaaccaccgc
     4021 tgagtctatc tggaccggcc caaacgtctc ttcgatggat ctctgaaaac cgtgggcatc
     4081 cgtgttgtcg ccgatgagac cctctggcga gggaatcacg gtattgacgg cgccgatacg
     4141 ctgtgccgtc tcggtcacgc ggtcgagaaa cggcatgaca gcctgtttat gaggcacggt
     4201 gacatttgca ccaagcacat ccggaagtcg gaggctggca acccggtccc ctaggccggg
     4261 gagcggtgtc ggccagagct cgtagaacgc atcgatgccg agctcgccga atgccgcgtt
     4321 gtgcatatgc ggcgaaagtg agtgctcgac cggatcaccg ataaccccga ctcgcttcat
     4381 ccgaaaattt ccctcacacg cgacctctgg aatgctcagg cagcaacctc acgggcccag
     4441 gaagtgaacc ctgcaaaggg cgatgcggtt ggacgttgtc cacttccccc acactcttcg
     4501 tattccggat gctcgataca ggtgttctgc aggtgctcct cgtaggtcgt gctgaagacg
     4561 tgcctgcccg tatcgtcgcc agctgtgacg aagtagaggt agtcgacctc cgctggctgc
     4621 agcacggcct gcagcgaggc gaaccccggg tttgcaatcg gcgcgggagg taacccctgg
     4681 aggtcatacg tgttgaactc actccctttg gcctgctcca gcaacgcggt gtcaagtacg
     4741 ggccaccact cctgttcggt tccgacgaca tactggagtg tcgggtccgc ctggagtggc
     4801 attcctgctt ccagccggtt gaggtaaaca gcagcgatct ggggccgctc ttccgggatt
     4861 gcggcctctc gctcaacgat cgatgccagc gtaacgacct cgtagatcga catcccccgt
     4921 gcctccgcct gggcgatcat ctctggggtg aattgggcct cgaagttcga cagctgcagg
     4981 ccgatgacgt cggccggagt cgcattctcg ccaacggtgt acgtatccgg gaagagaaac
     5041 ccctccaaag acccaccact ggggacactt tccaggaagg aatagttccc tcggaaaccc
     5101 tctacatcgt tggcggtggc gatgtactcg gcggcactgt ccagcatacc ggcctcctgc
     5161 agcacccgcg catactcctc aatgcgctgc ccttcgataa aggtcacctg gaacgattgc
     5221 gtgatttcgg cagccgcctc agtattttcg tctgggctgc tcgtatccgc gaccgtgatc
     5281 acgtcgatga tatcgggcac gctcatgccc atccggagtg tatagacgcc aggacggagt
     5341 tctacgccgc taaagcgcat acgggtctca aaataagagc gatactgcac cagctcggca
     5401 tccgttagct tttcggcaac ggtcccggtg tcatcctcct gggtcacctc gagcgtcacc
     5461 ggacggccaa tttcttcgga gcgggcacgg tcctgccagt aattgaagaa ccagacactc
     5521 ccgcccgcca cgacgacgga gagcacaaca atactgagaa ctttcagcgc cgagctgagc
     5581 atgcgaacca acggtcatta ctcctgggaa agggtgccgg cagcgaaaag agcgcggcga
     5641 gtatgatgag tgctgccaaa cgtgatccgt actcgtgtca cgcacgaaac atcaatgccg
     5701 gtaccattct acgtccatgc ttccatgaca gaccaaaggg acctccatcc tgaccgggca
     5761 gaatccggca aaccgcccct gatcgtgatc gtcgggccaa ccgccgtcgg caagacacgc
     5821 ctctccctga tgctcgcgga gcagttcaac ggcgagatcg ttaacgcgga ctcgcgctcc
     5881 ttctaccgcg gcatggacat cgggacagcc aaggtctcgc tggaggagcg cgaacgcgtc
     5941 ccacatcacc tgatcgatat cctggacccc aacgactccg tgtcgctggc gctcttccag
     6001 gacatggcgt acgcctccgt tcgcgacatc accgttcgtg ggaaaacatc cttcctggtg
     6061 ggaggcaccc cgcaatacgt caacgcggtc gtcgaaggct ggaacatacc ccgggtggag
     6121 ccggactccg acttccgcct gcttcttgaa cgcgaggcag cggagcacgg tttcgagaac
     6181 cttctggagc gccttcgcgt cgtagacccg gaatccgcca tccgtactgg gtcgaacgtg
     6241 cgccgcatca ttcgcgcatt ggaggtctac gagaagacag gacgcccgat gagtgagcag
     6301 cagtcgaagg gccccgctcc cttccgcgcc ctggaactgg ggctctggcg gccccgggaa
     6361 gacctccatc gccgcatcga cgagcgagtc cactcccaga tcgccgaggg actgttcgac
     6421 gaaatccgtt cgctcctctc gcaaggggtg tcaccggaga gcgcggcctt caccagcatt
     6481 ggttaccgtc aggggatgcc gtacatccgt ggggaggcca cggccgacga ggttgcggaa
     6541 cggatcaggc tcgatacacg gcgactcgtg cgtcatcagg agacctggtg gcggaaaaac
     6601 ccgcgcctgt tgcgcatcga cctgacacag ccggatgaga ccactcgagt gcaacgcgcc
     6661 gttgccgtgc accttggcga cgagggaggt tcataacgcg gtatagtttg gaaactattg
     6721 cagcgtcagc agagttcccc ggcatcctgg cccgagatca tctgcccggc gcggtcctca
     6781 acggccaaca cctcagcccc cagacaggag cttccatggt cacgaacttt ggtcataaca
     6841 tcgactccgc atttaccgga tatgcgcatc ctgaggcatt ggtgacgacg gattgggtcg
     6901 ccaaccactc aaatgatccg aaggtccgca tacttgagtc ggatgaagac atcatgctct
     6961 acgagatcgg tcacattccc ggggcagtga agctcgactg ggagaccgag atgcagcagc
     7021 aggtcaaccg ggacttcgtg gaccgggccg gctttgagcg cctctgcagc gagcggggca
     7081 ttaccccgga catgacggtt gtcttctacg gcgacaagaa caactggtat gcaacctatg
     7141 gcttctggct cttcaagtat ttcggccacg acgacgcccg ggtgatggac ggaggccgca
     7201 cgaaatggga ggccgaacag cgtccctata cgcgggagac accaaagatc gagccggcct
     7261 tttacacggc caaggatccc gacagctcaa ttcgtgcctt ccgtgacgag gtcctcaagc
     7321 acgtggagac cggtgcggga ccgctggtgg atgtacgctc gaaccccgag tacaccggtg
     7381 agctcctgca tgcaattggg tatccgcagg agggcgccca gcgcgctggt cacatcaagg
     7441 gtgcacgcaa cgtcccgtgg ggcacggcgg cgaacgccga cggcaccttc aagagcgctg
     7501 aggaactgcg cgagctgtac ggcaagcagg gcgtaacggg agacaagcca gtcatcgcct
     7561 actgccggat cggtgagcgc tccagccaca cctggttcgt cctcaaatac ctccttggct
     7621 acgacgatgt ccggaactac gacggctcgt ggacggaatg gggcagcctc gttggagtgc
     7681 cgaacgccgt cgggaacgag ccgggataat catccagaaa gagacaacag tgcttgaccg
     7741 acaggactat atcgagatta ttctcgacca ctatgagcag ccccgaaaca agcaccggat
     7801 agagaatgca agcgtccagc tcggtggtgg caatcccgga tgcggtgacc tgataacggt
     7861 ctacctcgac attggcgagg atgaccgcat tcgggaagca agcttcgaag gcgaaggatg
     7921 caccatctcc caagccggtg gctccatcat cgcggagctt attgagggca tgacgcttga
     7981 agaggtgaag gctctcggca aggagacaat gattgaggag atcggcgagg atgcggtgaa
     8041 gagtcgcgtc cggtgcgcca cccttgccct cggtacggca caggcggccg ttgacgccta
     8101 tatccgagac cggcggcgac agcaccaagg tcttgatacc ctccctgagc cggagcagtt
     8161 ccagaactga gcgtgcctgc atcatagcag gcacgaaaga acgcttccat tgcatgatga
     8221 cgcgccgacc tgtgtgaagg atgttgcggg gtgcatgaac cggaggattc atccctggct
     8281 cccggggcaa taccgcagcc gcgcccgcgc gagctcgcca tgagcgcggc atggaacgcc
     8341 ggtctcaccc gctccgtcac tacctcggac gggcaagagg tttcaatcat ttttcatggc
     8401 cactggagcc atggctttgg cccagatttc ttggacgcaa tgctccattt tggcgacgat
     8461 gggtacaaga ctggcgcggt agagatccac actcgctcca gtgactgggt agcacacggc
     8521 caccacctgg acgagcgata caacgcggtc attcttcaca tcgtcacgat cgatgacttg
     8581 ggtgagaccc gccgcgccga cggcaggcgc gttcccgtcg ccatcctgtc cataagtgac
     8641 gacatcctgt ttgcgattga tcgccacttg ccggagatct ggtcggagct agggaggaca
     8701 gtcacacctc cttcaccgtc cccatcctcg tctccgcccg gtccaatctc cgcctcgatg
     8761 tggtagctgt cattgaagag ctccatccac cactcggtac cctccggcca cggcgggttc
     8821 gcctcgagta agagtttgcc ctgtgcgcgg ccccgcttct caaccgtcag ccggacaccg
     8881 agccgatgta gcaactcccg cttctcggcg tagccccacg tccctacccc gtcaccatag
     8941 gcccgaaccg tatcccagaa cacccccatc gccttctccg ccttcgcagc cagctctgag
     9001 cggcgtaaag cttcctcgcg ctgcgtcacc aaggcgtcgc gccgggttgc gagggcgttc
     9061 agcttctcca cgaccgggtg tgctgccgag ccactcagcc gagtgagggc ttgaaccagg
     9121 ttgtcctgtt cgctttctag cttcgctaag ccacggtcga acgcgccaag gtcctcctga
     9181 acggagttta ggaccaactc ccggctccgt tcagcctcat ggaagagcac gcccgggtcg
     9241 cctagccatc cctcgaccct tccccacacg aactggtcaa ccggcccggc cttgacggac
     9301 caccccttgc attcaccctt tcctccccaa tagcgactgt tgcacttgta gaagattttg
     9361 tgtcttctgc ggctgacgtt catccggttt ccgcagtatg cgcagtaaat gaagcccgcg
     9421 cgcagcaacg cgtcctcggc cttgtcgtaa ggtcggctgt gctcgatgcg attcttcgcc
     9481 agcctttcat tggcccgttg ccatgtttca gccgaaatta gtgggggtgc cgcgtccttg
     9541 ataagcaccc attccgattc aggacggatg ctctcgcgct tacggctggt gtgctcgtcc
     9601 ttctcgacac ggtagcggtt ggcgtagacc tgccccttgt aggcggggtt cgctagcatg
     9661 cgactgatct gtcgcgcctg ccaccgtttg ccgctcgtgg tggtcatgcc atcatcattg
     9721 aggcgcatgg caattcgccg gagaggaacg ccggaagccg cttcctcgaa gacacggcgc
     9781 acgatccagc tgacggcttc atcctcgaca tggcgagtct tcgccgattt cgagggatcg
     9841 tcccactgat acccgtagag cggatgccct ccgggcagta gcttcccctg ctccttcgcc
     9901 cgtgctctgc acccagtggc catccgctg
//
LOCUS       DASYWM010000056         3886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_339552, whole genome shotgun sequence.
ACCESSION   DASYWM010000056 DASYWM010000000
VERSION     DASYWM010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339552"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..1392)
                     /locus_tag="VGR29_02160"
     CDS             complement(304..1392)
                     /locus_tag="VGR29_02160"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV2072422.1"
                     /translation="MQTTTAISVIPGVPESLERIEVVVPEPGPMEVLVDGIRCGICGT
                     DREIIEGHIGLPPENQQHLVLGHEVLGRVHAVGSGVTDLRPGELVTATVRRPDGCPSC
                     QAGQPDMCQWQQYTEHGIKGRNGFLAGQFITERPYVIPVDAHLEETGVLIEPLTVVEK
                     AVRQAMLIQRRMAVWEPRTAIVAGAGPIGILGTLLLRSIGLEVYTVARTPAPNIHSDV
                     VTRAGAHYVSTGEETIAALAQRLGNVDIILESTGASMVAFEAMTALGINGVLVLLSVT
                     DTATMMEVSTGGINNGLVGGNKVVVGSVNSGIEDFEAAVVRLDRFGQLWPGLAASIIT
                     HRIPFNGDLSGVLHEPKDAIKVVIEFDL"
     gene            1603..1788
                     /gene="rpsU"
                     /locus_tag="VGR29_02165"
     CDS             1603..1788
                     /gene="rpsU"
                     /locus_tag="VGR29_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV2072423.1"
                     /translation="MRVEIRDNEGFEQLLRRFNKGIERSGIIREHRRGLRFISVQEEE
                     RAKRRKAERRRRRNQAK"
     gene            1959..2819
                     /locus_tag="VGR29_02170"
     CDS             1959..2819
                     /locus_tag="VGR29_02170"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEV2072424.1"
                     /translation="MKILISADMEGTCGVSSWVHVTPPEGAGSGEPANQTEYERARLR
                     MTREVNAAIEGALAGGATEVIVNDSHDGMRNLIAEELRTEAQFISGNDKFLGMMQGVE
                     LEGISGVFYTGYHAKAGTPQAPLAHTWSGWLNDVRFDGVSTGEFGINAAIAGHFGVPV
                     VLVTGDEKAVAQTQEFLGDQVEGVAVKQGISTFAAMHLHPEKAQRSIREGAQRAMGQI
                     SGAKPYVLANGVTVELEFDHQARADQAELLPSVKRAGERAIAFQPADGLEFNVMFRAA
                     CKLASIRMSP"
     gene            complement(2947..>3886)
                     /locus_tag="VGR29_02175"
     CDS             complement(2947..>3886)
                     /locus_tag="VGR29_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072425.1"
                     /translation="DLSMPWFKIRKQNKEKESQEPPATEKTGAPEPPLQSAEATPTLP
                     PHMQRLVDERSKVRPRRQASVEERRASYDRKRLAIQYDIEQGYLATEPENPWTHRIEL
                     LTEALGTIEEDLKAESEVPPAPFYPLPPTPISDITIERDRGIAVSFTIDGHRFAFAET
                     LDWAERGHRVSQPEFRATVGDVEKLIPAEKPPELREPLEQHLTDSLAVFATDLRDRAL
                     DNEPMPTSATLADLAGPCKICGGWADWRGRCDACTNRKAREQSLRLEQTRLLRERANE
                     AEERHRMAERLPIARRRMADLEAEIAAFERSLREET"
BASE COUNT          726 a         1139 c         1234 g          787 t
ORIGIN      
        1 catggttcac agcgcgcgca atctgagtga tgagacgacc acgaccctct tctcaggtct
       61 aatggagcag ggccagccat tgaccagctg cgttgatgaa gcatcaccaa tggcacgttt
      121 aggctactaa ttcaagacca ggtgcaccta tccgtgcaga tgcacgcata gcgatcgaca
      181 agagtggaag ctaggtgccc ttgtcgcact aggggcacct ggttgtttgg cattggtcgg
      241 ctttgcggga tgggagatag aggcagaacg cgcgcctggg tgacggccac ccttgcctcg
      301 ccgttataag tcgaactcga tgacaacctt gatcgcgtcc tttggctcgt gcagcacacc
      361 gctcagatct ccgttgaacg gaatgcgatg ggtgatgatc gatgcggcaa ggcccggcca
      421 aagctgcccg aagcgatcaa gacgcactac cgcggcctcg aagtcttcga tccccgagtt
      481 gaccgatcca accaccacct tgttgccgcc cactaaccca ttgttgatcc cgccggtgga
      541 cacctccatc atcgtagcgg tgtctgtgac gctcagaaga accagcacac cgttgatccc
      601 aagcgcggtc atggcctcga acgctaccat gctcgccccg gtactctcca ggatgatgtc
      661 gacgttcccc agccgctggg caagtgcggc gattgtttct tccccggtcg aaacgtagtg
      721 cgcccccgca cgggtcacga catcggaatg tatgttcggt gccggtgttc tggcgaccgt
      781 atagacctca agcccaatcg agcgcaggag aagtgtcccc aggatgccga tcgggcccgc
      841 cccggcgacg atcgcggtac ggggctccca gacggccatg cggcgctgga tcagcatcgc
      901 ctgccggacg gccttctcaa ctacggtgag gggctcgatg agaacccccg tttcctcaag
      961 atgagcgtcg accgggatca cgtagggccg ctcggtgatg aactgcccgg cgaggaaacc
     1021 attcctgccc ttgatgccgt gctccgtgta ttgctgccac tggcacatgt ccggctgtcc
     1081 tgcctggcag gaggggcacc cgtccggccg gcgcacggtg gcagtgacca attcgccggg
     1141 acgtagatca gttacgccgc tccctacggc atggacccgt ccaagtacct cgtgcccgag
     1201 tacgagatgc tgctggttct cgggtgggag gccgatgtgc ccctcgataa tctcccggtc
     1261 ggtgccgcag atgccacagc gaatcccgtc gaccagcact tccattggcc cgggctcggg
     1321 aaccacaacc tcaatgcgct cgagggattc gggcactccc gggataacgg atatggcggt
     1381 ggtagtctgc atcggcgatg gctccagtag tgatgttcgc tcaacgaaat gacctccatc
     1441 atacggcctg gcacggcagg tgggagcgat gacgtttttt ggcgtatact tgggcatcgg
     1501 gtcaagttgt acccattcgt gttgttgcgc cttacgtggc acgcaggaca cgaagccgtc
     1561 agttttgtca ttacacgcgc ttgaaggtgg tgataagcat ttatgcgtgt tgagattcga
     1621 gacaacgaag gctttgagca gctgctccgc cgattcaaca agggaatcga gcgctccggg
     1681 atcatccggg agcaccgtcg tgggctgcgc ttcattagcg ttcaggaaga agagcgcgcc
     1741 aagcggcgta aggccgagcg gcgtcgccgt cgtaaccagg cgaagtaatc tgccgatctt
     1801 tttcggcggt tgagttcatc ggttacgggc atcacgttat gcacgaacca gaacgggtag
     1861 cgtgagagga cgaaagtccc tctccctgcc cgtttttgct acccgcttgt atagatgcgc
     1921 ataaagaccc ggcgctgttt cagaggagag ggcaacagat gaagatcctg atttctgcgg
     1981 atatggaagg aacctgtggt gtttcgtcgt gggtgcatgt cacacccccg gagggcgcag
     2041 gatcaggaga gccagcaaac cagacggaat acgagcgggc ccggcttcgc atgacccgcg
     2101 aggtcaacgc agcgatcgag ggtgcactgg cgggaggggc aaccgaggtg attgtcaatg
     2161 acagtcacga cggcatgcgg aacctgatcg cagaggagct ccgcacggag gctcaattca
     2221 tctctggcaa cgacaagttc ctcgggatga tgcagggggt cgagcttgag ggcatcagcg
     2281 gggtgtttta tacggggtat cacgcaaagg ctggcacgcc gcaggctccc ctcgcgcata
     2341 cctggtccgg ctggctgaac gatgtccgtt tcgacggtgt ctctaccggt gagttcggca
     2401 tcaacgcggc gattgccggg cattttggcg tcccggtcgt tctggtgaca ggcgatgaga
     2461 aggctgttgc ccagacgcag gagttccttg gcgatcaggt cgagggcgtg gcggtgaagc
     2521 aggggatttc caccttcgcg gccatgcacc tgcacccgga gaaggcgcag cggtcaatcc
     2581 gggaaggagc gcagcgcgcg atggggcaaa tttccggcgc aaagccctat gtcctcgcga
     2641 acggcgtaac cgtcgagctg gagttcgatc accaggcgcg ggcggatcag gcagagctgc
     2701 tgccaagtgt gaagcgcgca ggtgagcggg caatcgcctt ccagccagcg gacggtctgg
     2761 agttcaacgt catgttccgg gcagcctgca agttggccag catccggatg tccccctagg
     2821 ggattttggc gcagactttc tgggcgggag catccgagag gcaacgtcct gccgtcgctt
     2881 gtagcagcta cagtacgggt cggccgcgag ggctcaccgt gccgacgctg ggataaccgg
     2941 tctcctctat gtctcctcgc gtaacgagcg ctcgaaggcc gcaatctcgg cctccagatc
     3001 cgccatgcgg cgtcttgcga tcggaagacg ttcggccatg cggtgacgct cctccgcctc
     3061 gttggcgcgt tcgcgcagca gccgggtctg ttccaggcgc agcgactgtt ctcgtgcctt
     3121 gcgattcgta caggcgtcac accgtccgcg ccagtctgcc cagcccccgc aaatcttgca
     3181 tggcccggcg aggtcagcca gtgtggcact ggtgggcatc ggctcgttgt ccagcgcgcg
     3241 atccctgaga tccgtggcaa agaccgccag gctgtcggtg agatgctgct caagcggctc
     3301 ccgcaactct ggtggcttct ctgcagggat cagtttttca acgtcaccga ctgttgcccg
     3361 gaattccggc tgcgataccc ggtgcccacg ttctgcccag tcgagcgttt cggcaaaggc
     3421 aaaccggtga ccatcaatag tgaacgaaac agcaatcccg cgatcccgtt caatcgtgat
     3481 gtcactgatc ggggttgggg ggagcgggta gaagggcgcc ggagggacct cgctctcggc
     3541 cttgaggtcc tcttcgatgg tgccgagagc ctccgtgagg agctcaatcc ggtgtgtcca
     3601 gggattctcg ggttctgtgg cgagatagcc ctgctctatg tcgtactgga tcgcaagccg
     3661 cttgcggtcg tatgaggccc ggcgttcctc caccgatgct tgccgccggg ggcgtacctt
     3721 gcttcgttca tcgaccaggc gttgcatgtg cggtggcagg gtaggggtgg cttcagcaga
     3781 ctggagaggt ggctcaggag cccccgtctt ctcggttgct ggtggctcct gcgactcctt
     3841 ttccttattc tgtttgcgga ttttgaacca aggcatagat agatcc
//
LOCUS       DASYWM010000057         5567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_339673, whole genome shotgun sequence.
ACCESSION   DASYWM010000057 DASYWM010000000
VERSION     DASYWM010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339673"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="VGR29_02180"
     CDS             complement(<1..1324)
                     /locus_tag="VGR29_02180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV2072426.1"
                     /translation="MHPSLLVSGGPILTMHPANPEVEAVGIINGRIVATGDRADVRKE
                     LGSRIEEIDLNGRMALPGFHDAHAHVMGVGYALSQIDLSSKAVSSIDDILVAVKERVK
                     STDTARWILGQGYDQANLAENRHPTREDLDQVAPNHPVALWRSCHHIMAVNSRALELA
                     GINIDTPDPSDGTIDRDSSGIPTGVLRESACNTVNRAIGLPDEDEIATALDAGGQAFR
                     QYGITSVAEAGIRRAEEMRAYQRLHDQGRLALRTWLMMIIDETLDELISLGISSGFGD
                     AWMRIGNAKLFSDGSIGGRTARMSRSYEGENDNYGLLMMPPEEISERVVRAHKAGFQI
                     GIHAIGDAAINHVLDAYEEAQRQLPRLDPRFRIEHCSIIDETIIDRIQQLGVIPIPGT
                     TFLYDMRPVYLQNLGEQRVRYAYAMRSFRVRGIIAAASSDAPVSSQNPM"
     gene            complement(1329..2099)
                     /locus_tag="VGR29_02185"
     CDS             complement(1329..2099)
                     /locus_tag="VGR29_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072427.1"
                     /translation="MIDASSPVRWPAGHRAALSVVVHVPGVGLEADTARHPGLVGVDY
                     TATGLQRILETLADVDVSATVAFTAEATSGSPQLLRRAVELGHEVAASACSATASIPD
                     LVETISGITGSPVNGLIEQLPGLPSGDQDERFGDETGNAWRITGAGGDLPNSGGDPNS
                     TIIPVSPYLIDMTWLSPSRPLPPSSMLEAWSLALAAHRADSSFMPVLLHPHIVGRPGL
                     ASTITRFLDEVVAAGDVWIARLDHVARAWHELAPRREE"
     gene            complement(2096..3346)
                     /locus_tag="VGR29_02190"
     CDS             complement(2096..3346)
                     /locus_tag="VGR29_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEV2072428.1"
                     /translation="MPKRERNLSQLRPATRAVHGGERMDIRPAIPTVNPIHFSTSYVF
                     ESSEDLDAVFVDPSAGYAYGRFGNPTVRALEEAMAAVENTEAAIAYPSGMAAINGVFT
                     YLAKPGDHVVVSRDVYGATLTLLNGHFTEMGIISHFVDITDLAAVQAAAEEVKPKVIY
                     AETISNPLIKIADIRGIVDIGRDCGAKVVIDNTFASPALIRPADLGADLVLHSTTKFL
                     GGHGDVLGGVVSGGAEDVTGLRNIARINGAVPGPFDAWLCSRGLKTLFLRMREHSANA
                     RKVVAWLQQDPRIERVYYPGLEDDSLADQFLSDDRGGMVAFEVTGFGKEEAYRYLESL
                     RLVEPATTLGDVYSLSLYPAASSHRGLSPEQRADWGIGDNLLRLSIGIEDARDIIEDI
                     DQALTAATEATSAAESGRQELYVS"
     gene            3665..4006
                     /locus_tag="VGR29_02195"
     CDS             3665..4006
                     /locus_tag="VGR29_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072429.1"
                     /translation="MAESARPAAGRSHGEQCQVDHPADGHCPRIIFERASAGPLGGGM
                     YRVAAIVQNTGFLPTFVSERGKETGRIKPVKITLDAAGGVTIVSGKREQELGHLDGRA
                     NQYGSLGVPGQ"
     gene            4147..5043
                     /locus_tag="VGR29_02200"
     CDS             4147..5043
                     /locus_tag="VGR29_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV2072430.1"
                     /translation="MSRVTEDQFLGSLLGVAIGDALGLPVEGLAADKILERYGAFDGY
                     LEIEDAGDGEPITGMVSDKSEVVLCIVESLTTNDGILNPENINARLRFLVNGNSRKWM
                     SDAVINGIEKAAEQNGLVDADLASAPEAAVALRGVPIGLLHSIGGWDQDALQKDAETV
                     TRLSHGGESAIRLTAQVARATALAARSAQGEDHSHKVPVPENEEPLAQQIASIVEEVK
                     LATTFEDAVFPVVHAGGSASALGAIAGGIAGAKFGASGIPQNLIDELDARIYLSMAAP
                     WYHKTVMRRAGTVIDLRAVDDE"
     gene            5194..>5567
                     /locus_tag="VGR29_02205"
     CDS             5194..>5567
                     /locus_tag="VGR29_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEV2072431.1"
                     /translation="MADKRDYYEVLEISRTASDTEIKRAYRTLARKYHPDVNKAPDAE
                     DRFKEVNEAYEVLSNVDRRAAYDRFGHAAAAGAGAGDPFGFGGAGSPFGDLFETFFGG
                     AAGGANGRRSAPVRGADIQVSV"
BASE COUNT         1104 a         1607 c         1792 g         1064 t
ORIGIN      
        1 tcattgggtt ctgtgaagaa accggcgcgt cactgctggc tgccgcaata atgcctcgta
       61 ccctgaagct cctcatggcg taggcataac ggactcgctg ctcaccaaga ttttgcagat
      121 agaccggacg catatcatag aggaacgtcg ttcccgggat cgggatcaca cccagctgct
      181 ggattcggtc aatgatcgtt tcgtcgatga tggagcagtg ctcgatgcga aaccgcggat
      241 ccaggcgtgg cagctggcgc tgagcttcct catatgcgtc cagtacatgg ttgatcgccg
      301 cgtcgccgat ggcatggatg ccgatctgga agcccgcctt atgggcacgt acgacgcgct
      361 cgctgatctc ctccggaggc atcatcaaca agccgtagtt atcgttctca ccctcgtatg
      421 acctggacat gcgggccgta cggcccccga tagagccatc cgagaagagc ttggcattgc
      481 cgatcctcat ccaggcgtcg ccgaacccgc ttgagatccc gagcgagatg agctcgtcga
      541 gagtttcgtc gattatcatc atcagccagg tgcgcagcgc aagccgaccc tggtcgtgca
      601 ggcgttggta tgcgcgcatc tcctcagccc ggcgaatccc agcctcggca accgaggtga
      661 tcccgtattg ccgaaatgcc tgtccgcctg catcaagggc cgtggcaatc tcatcttcat
      721 cgggcaagcc gatggctcta ttgacggtgt tacaggccga ctcccgcaag acgccggttg
      781 ggatgccgct ggaatcgcgg tcgatcgtac cgtcggaggg gtccggtgta tcgatgttga
      841 ttccggcaag ctcgagggct cgcgagttga ctgccatgat gtgatggcag cttcgccaga
      901 gcgcaaccgg atgattcggt gcgacctgat cgagatcctc gcgcgtcgga tgccgattct
      961 ccgcaaggtt cgcctggtca tacccctgcc cgagaatcca tctcgccgta tcagtcgact
     1021 tcactcgctc cttcacggcg acaagaatat cgtcgatcga ggagacagct ttgctggaga
     1081 ggtcgatttg cgagagcgca tagccgaccc ccatgacgtg ggcgtgcgca tcatggaagc
     1141 cggggagagc catgcggccg ttgagatcaa tctcctcgat tcggctcccc aattccttcc
     1201 gaacgtcggc acggtcgcca gtggccacga tccgaccgtt aattatcccg acagcttcga
     1261 cctcaggatt ggcaggatgc atagtgagga tcggcccgcc ggacaccagc agagatggat
     1321 gcacggtgtt actcctctct tcgcggcgcg agttcgtgcc aggcacgggc gacatgatcg
     1381 agccgggcga tccagacgtc accggcggcg acaacctcat cgaggaagcg ggtaatcgtg
     1441 ctggcaaggc cagggcggcc gacaatatgg ggatggagca agaccggcat aaaagagctg
     1501 tccgccctgt gcgcggccag ggccaacgac catgcctcca gcatggagga gggtgggagc
     1561 gggcgcgatg gcgagagcca ggtcatgtca atcaggtagg gagagacggg gatgatggtc
     1621 gagtttgggt ccccaccgct gtttggaaga tctccgccgg ccccggttat ccgccaggcg
     1681 ttgcccgtct cgtcgccaaa gcgctcgtcc tgatcccccg atggaaggcc ggggagctgc
     1741 tcgatcaggc cgttcactgg tgagccggtg atgcctgaaa ttgtctcaac gagatccggg
     1801 atagacgccg ttgccgagca agcgcttgcc gccacctcat gtcccagctc aacagcgcgg
     1861 cggagcagct gaggcgatcc gctggtcgcc tcagcggtga aggccactgt ggcggagaca
     1921 tccacatcgg ccagcgtctc aaggatgcgt tgcagccctg tggcggtgta gtcgacaccg
     1981 acaagacccg gatgccgggc ggtgtccgct tccagaccga cgccgggaac gtgcacgaca
     2041 acggagagcg ccgccctgtg gccggctggc catcgcacgg gcgaagacgc atcgatcatg
     2101 agacgtaaag ctcctgtctg cctgattcgg cggcggacgt tgcctcggtg gctgccgtca
     2161 acgcctggtc gatatcctcg atgatgtcgc gggcgtcctc gatgccgatg gagagccgca
     2221 gcaggttgtc accgatgccc cagtcagctc gttgctccgg gctgagtcct cgatgagagg
     2281 aggctgcggg atagagggag agcgaataaa cgtcaccgag ggtcgttgcg ggctcgacca
     2341 gtcggagtga ttcgagatag cggtatgctt cctccttgcc gaaaccagtc acctcgaatg
     2401 cgaccatgcc gccacggtca tcggacagaa actggtcggc cagcgagtcg tcctcgaggc
     2461 ctggatagta cacgcgctcg attcgcggat cctgctgcag ccatgcgacc accttgcgcg
     2521 cgtttgcgct gtgctcgcgc atgcggagga agagcgtctt aagcccgcgg ctacagagcc
     2581 atgcatcgaa cggacccggc accgcgccat tgatgcgggc gatatttcgc agccctgtca
     2641 cgtcctcggc accaccggag acgacacctc cgagaacgtc accgtggccg ccaagaaact
     2701 tggttgtcga gtggaggacg agatccgcgc ccaagtctgc gggacggatg agcgctggcg
     2761 aggcaaaggt gttgtcaatc acaacctttg ccccgcaatc gcgaccgatg tccacgatgc
     2821 ccctgatatc ggcgatcttg ataagcgggt tggatatcgt ttcggcgtaa atgaccttcg
     2881 gcttcacctc ttcggcggct gcctgcacag cggcgaggtc cgtgatgtcg acgaagtgac
     2941 tgatgatgcc catctcggtg aagtggccgt tgagcagggt caaggtggcg ccgtagacat
     3001 cgcgcgagac aacgacgtgg tcgccgggtt ttgcgagata ggtgaagaca ccattaatgg
     3061 ccgccatacc agagggatag gcaatggcgg cctctgtgtt ttcaacggcc gccatcgctt
     3121 cttccagtgc gcgcactgtg ggattcccga agcggccata ggcatacccg gctgacgggt
     3181 ctacgaacac agcatccaaa tcctcgctcg actcgaagac ataggaagtg ctgaaatgga
     3241 tcgggttgac ggtggggatt gcgggacgaa tatccatgcg ctctccgccg tgtactgcac
     3301 gagtcgcggg gcgcagttgt gacaggtttc gctcgcgctt cggcatgtgt gattgttcct
     3361 tattgaagta cgtgccaacg aatcaatgag tggagtctag agcggcgctg gaagggcgtc
     3421 aacgaggcgc aattgataga ttgacatggt gcggccggtg cacataccgt gaccaccata
     3481 tgagttggtc cgccggaaga aggggcaggg gctcgcatgg ctgtgacaca gggcatatca
     3541 aagtgggtcg cgacatcggc cgactaccgg ccagaccgat actaccgcta tgagcacctg
     3601 accgagttgt tgcacaactg ggcgtcggcg aggtagggat tggcggccgg gattacaagt
     3661 tcgcatggca gaatccgccc ggcccgctgc tggaagaagt cacggcgagc aatgccaggt
     3721 tgaccatccg gcagatgggc actgcccccg gattatcttc gagcgagcgt cggccgggcc
     3781 tctcggcgga ggcatgtatc gggtggcggc gattgtgcag aacacagggt tcttgccgac
     3841 attcgtcagc gagcgcggca aggaaacggg acggatcaag ccggtgaaga tcaccctcga
     3901 tgccgcaggc ggggtaacga ttgtttccgg caaacgggag caggaactcg ggcacctgga
     3961 cggccgcgca aatcaatacg gttctctggg agttcccggc caataggcaa tcctctcccg
     4021 tgggcgcgtc gaatggttta tctctggatc accggggagt acggttgagt tggttgcaac
     4081 ggcaagcaag gcgggcacgg ttcgcatagg cgtagcgcta ccagacgacg acaaaggaca
     4141 taccgaatga gccgagtaac agaagaccag ttcctgggat cgctcctggg agttgcgatc
     4201 ggggacgcgt tggggttacc cgttgaaggc ctggcggcgg acaagattct cgagcgctat
     4261 ggtgcgttcg acggctatct cgaaatcgag gacgcgggcg acggcgagcc gattacgggc
     4321 atggtttccg ataagtccga agttgtgctg tgcatcgtcg aatcactcac caccaatgac
     4381 ggtattctca accccgagaa catcaatgcg cggcttcgtt ttctcgtcaa tggcaactca
     4441 cgcaaatgga tgagcgatgc cgtgattaat gggattgaaa aggctgcgga gcaaaacgga
     4501 ttggtcgatg ccgatcttgc ctctgcgccg gaggccgctg tggcgctacg cggcgtaccg
     4561 atcggcttgc tgcattcaat tggaggctgg gaccaggatg cgttgcagaa ggacgcggag
     4621 accgtaactc gcctctcgca tggcggtgag agtgcgatcc gtctcaccgc ccaggtggcc
     4681 cgggctactg cattggctgc ccgctcggca cagggggaag atcattccca caaggtgccg
     4741 gttcccgaaa acgaggagcc gctggcgcag cagatcgcca gtatcgtcga agaggtaaag
     4801 ctcgcgacaa cgtttgagga tgccgtcttc ccagttgttc acgcgggtgg gagcgcaagc
     4861 gcactcgggg ctattgcggg cggtatcgcc ggagcgaaat tcggggcaag cggaataccg
     4921 cagaacctga tcgacgagct ggacgcccgg atctaccttt caatggctgc tccctggtat
     4981 cacaagacgg tgatgcggcg ggccgggact gtgatcgacc tgcgcgcagt cgacgacgag
     5041 tagctgaccg acgggcgccg aaaatccgtt agccgaggtt gcaggccccg ctatacttat
     5101 ctgtaatgag acgtactcgc cgtgcgtgtc ctcgcgtacg gcctgttttg cgcgtccgcc
     5161 ctcctggcgt cgcttcacat tcatgattgg gttatggcag acaaacgcga ctactatgag
     5221 gttctggaga tctcccggac cgcctctgat accgagatca agcgcgcgta ccgcacgctc
     5281 gcccggaagt accaccctga tgtcaacaag gcgccggatg ctgaggaccg attcaaggag
     5341 gtcaatgagg catatgaggt actctccaat gttgaccggc gcgcagccta cgaccgcttt
     5401 ggccatgccg ccgctgcggg agcgggagcc ggcgatccct tcggattcgg gggtgccggt
     5461 tcgcccttcg gcgatctctt tgagacgttc ttcggcggag ccgctggcgg cgccaatggc
     5521 aggcgctcag ctccggttcg cggggccgac attcaggtat cggtcaa
//
LOCUS       DASYWM010000058         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_245858, whole genome shotgun sequence.
ACCESSION   DASYWM010000058 DASYWM010000000
VERSION     DASYWM010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245858"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..446
                     /locus_tag="VGR29_02210"
     CDS             171..446
                     /locus_tag="VGR29_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072432.1"
                     /translation="MRYERWRESMPHVTGAAPVPKLHLAPQRSGVLLGPVMTALGALE
                     WRTERYVERLKLTEDDAHAMRSANTALRKALIDIAKLRDGLLRGEAE"
     gene            443..1159
                     /locus_tag="VGR29_02215"
     CDS             443..1159
                     /locus_tag="VGR29_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2072433.1"
                     /translation="MTTERKKRVLITGAGGRIGRNLAERLGDRYALRLHYHARVPDDA
                     PVTDAVAGEISRYDEIAPALDGMDAVVHLAGEPSTVASWEEVHEANILGTYNVFEAAR
                     AAGVGKIVFASTNHVMGMYDRDGEWPVYSNQPVRPDSLYGVSKAFGENLGRHYHDQHG
                     LSVICLRIGWYLPEPKDDISAWMWLSPRDCAQIVWRAIESPLGFGTFYAISANSDRHW
                     DITDTIEHLGYRPEDDAARA"
BASE COUNT          302 a          485 c          505 g          307 t
ORIGIN      
        1 tgcgccacgc tcacgcaaat aggcgatgac ggtgcccctg ggcatcggca ccaccgcggg
       61 ccaggaggaa ggcatgccgt agaatacggt ggtccgccag tcgtcatctg tggaaggggt
      121 ttgctggaag gcggacctct cgcccaggcg ctggtggcca tgagcctgcc gtgcgatacg
      181 aacgttggag agaatcgatg ccgcatgtaa ctggtgccgc tcccgtcccg aagctgcacc
      241 ttgccccgca acggtccggt gtcctgctag gtcctgtcat gacggcgctg ggcgcgctgg
      301 agtggcgaac tgaacgctac gttgagcgac tcaagttgac ggaggacgac gcccacgcca
      361 tgcggtcggc gaataccgcg ttgagaaagg ccctgatcga tatcgcaaaa cttcgggatg
      421 gtctgctccg aggggaagca gaatgaccac tgagcgaaag aaacgcgtct tgatcaccgg
      481 cgcaggtgga cgcatcgggc ggaacctggc cgagcggctc ggagaccgct acgcgctgcg
      541 attgcactac catgctcgag tccctgacga cgcgccggtg acggacgccg tggcaggtga
      601 gatcagccgg tacgatgaga ttgctccggc gctggacggg atggacgcag tggtccacct
      661 ggccggcgag ccgagcactg tcgcgtcttg ggaagaggtg catgaggcca acatcctcgg
      721 cacctacaac gtcttcgagg ccgctcgcgc cgcgggcgtc ggcaagatcg tcttcgcctc
      781 gaccaaccac gtcatgggga tgtacgaccg cgatggcgaa tggccggttt acagtaatca
      841 gcccgtacgc ccggattcac tctatggggt ctcgaaagcg ttcggcgaga acttggggcg
      901 tcactatcac gaccagcatg ggctatccgt gatctgcctg cggatcggct ggtatttacc
      961 cgagccgaag gacgatatca gtgcctggat gtggctcagc ccccgcgatt gcgcccagat
     1021 cgtgtggcgc gccatcgaga gcccgcttgg ctttggcacc ttctacgcga tctccgccaa
     1081 cagtgaccgg cattgggata tcaccgacac catcgagcat ctcggctacc gtccggagga
     1141 tgatgccgca cgggcgtagc ggtccgtgca tgcgcacagc gatcgccgca gtcagggaaa
     1201 ggatagcagg atagacgacg cggaacctct cgcggcggcg agaggtgctg gctcgggata
     1261 cgctatgcgc ggtccaggtg tttgttggga ggctgagtaa tacacactca gccggctcag
     1321 gaaggtcgta gagcattcac ccgatctgcc tataaacccg ccgagaaccg ttttcgtagc
     1381 catccatcat ccggtgacaa cgaggacaat gcagtagctc ctagaagacc ctgccatgat
     1441 gtcgagtccg gggattatcc atcctcatcg tcatcctcat cgtcgttgcc cttgccccga
     1501 tcgtcgtcgt ctttcttctc cttttcctcc ttgtcgtcct gccgattgtc ttcgctatta
     1561 ccccggttgc cgccttcgtc ggactggatg gcgaccgcg
//
LOCUS       DASYWM010000059         4988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_639892, whole genome shotgun sequence.
ACCESSION   DASYWM010000059 DASYWM010000000
VERSION     DASYWM010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639892"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..834)
                     /locus_tag="VGR29_02220"
     CDS             complement(46..834)
                     /locus_tag="VGR29_02220"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2072434.1"
                     /translation="MSTLPPYVVVSRPVRSGEERISGSRSRTTARSGPMREIGIVSLA
                     VLLYFLVRGMMDSTAALAGQHAHWLVDLERRLGIFHEPGMQRWALGQDWLVWAVNAVY
                     IYGHWPVLIATMGWLIWKRRDQFPVYRSALLISGAIGLVVFVVFPMAPPRFLPEAGFV
                     DTVTLHTNAYRVLQPPSFTNQYAAMPSLHVGWNLLMGIAIFRTTRHPLWRTFAIVMPL
                     AMYIATVLTANHFLLDGVVGSVIAINGLALASRISALKRERDVF"
     gene            complement(1437..1802)
                     /locus_tag="VGR29_02225"
     CDS             complement(1437..1802)
                     /locus_tag="VGR29_02225"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEV2072435.1"
                     /translation="MEKLIGRINGSAFEGWLDRWGGKALFVIRAIPGTPSDFISYLAG
                     LTNMRRRTYVLATFLGYIPQSLLFAWLGDTAMGWFWWIVMAGFAVSALIGLAGWAIQR
                     RSNEAGRRRIRPRASATCI"
     gene            complement(1721..2215)
                     /locus_tag="VGR29_02230"
     CDS             complement(1721..2215)
                     /locus_tag="VGR29_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072436.1"
                     /translation="MQTIQQEFGDESAGTWWRGHRLALLPWITLLVVMGVAVVTYLLS
                     NPFREEVHQGVDVLTSGDHQRIREYLRGYGAWGPVVSIFLMTAQVIFAPIPASVVQLA
                     NGVVYGPLWGTLLNIAGQMAGQCWPSGSPDHLARARWRSSSAGSMEVPSKAGSIDGAA
                     RPSL"
     gene            2505..3695
                     /locus_tag="VGR29_02235"
     CDS             2505..3695
                     /locus_tag="VGR29_02235"
                     /inference="COORDINATES: protein motif:HMM:NF004636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2072437.1"
                     /translation="MATTFCIRNVQPAENDPVDVLVRDGKIASIGAGIVPDEDVEEID
                     GGGRLLFPGFVDAHTHMDKTLLGLPWHRHTAGPALLDKIEGERRLRQEEGIDAHQQST
                     RHALQAIASGTTHVRSFADIDTEWELKGFRGLMQTREDLRDSLTIQVVAFPQSGMLVR
                     PGTVELMKQALKEGAEVIGGLDPSTIDRDPAGHLDTIFGLAEKYDVDVDIHLHEPGEL
                     GAFSIDLIAERTQVLGMHGRVVISHAFCLGGVDDARLDGLIDKVLGSDIAIMSHGPSG
                     GRPAPPVGQLRAAGVRMCAGNDGIQDAWGALNRPEMLLRAYLIAYRNNFRRDDEIEDV
                     IDIITNGGADVIRVGDYGFAEGKAADLVLVEAENHVAAVVTQATPWLVMKRGKIVARD
                     GAIV"
     gene            3749..4306
                     /locus_tag="VGR29_02240"
     CDS             3749..4306
                     /locus_tag="VGR29_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2072438.1"
                     /translation="MSVTPESDQGTKEQSVPPGTRIGHVHLKVSDLDRAVRFYQEVMG
                     FDLIIRYRNQAAFLSAGGYHHHIGLNTWESLGGAPPPPGTTGLYHFAINYPERRDLAA
                     ALKRLLDHGWGIDGASDHGTHEALYLHDPDMNGIELAWDRDPSHWYRNGEPVFERNPL
                     KFASLLGELGDGEASKYLKALARYT"
     gene            4337..4888
                     /locus_tag="VGR29_02245"
     CDS             4337..4888
                     /locus_tag="VGR29_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2072439.1"
                     /translation="MPRALVRNATPDDATAIAAIHVASWQATYRGHMDDAYLDSLSVD
                     ERVGMWSQILEFPRTAANVLVAEIQGKVIGFYSFGRSRDSDLPGDTAVLHTIYVHPDL
                     IRSGIGIALLARAERAMREAGIDLVSLWVLRENIGARRFYERCGRSFNGEERREELWG
                     QPPTEVRYTRSLSSGDQRDIGGT"
BASE COUNT          991 a         1570 c         1511 g          916 t
ORIGIN      
        1 cccatgcagc ccgaaaatct ctcgcgtgag caggcgcgac gccggctaga acacgtccct
       61 ctcgcgtttt agtgcgctga tccgtgacgc aagggccagc ccgttgatag cgatgacgct
      121 gccaacgaca ccatccagca ggaagtgatt cgcagtcagg accgttgcga tatacatcgc
      181 gagcggcatg acgattgcaa acgtgcgcca aagaggatga cgggttgtgc ggaagatcgc
      241 gatgcccatg aggagattcc agccgacatg gaggctcggc atcgcggcgt actggttcgt
      301 gaaggagggc ggctgcagga cgcgataggc attggtgtga agcgtcaccg tgtcgacaaa
      361 gcctgcttcc ggcaggaacc ggggtggcgc catcgggaag accacgaata ccaccagacc
      421 aatggcgccc gagatcagca acgctgagcg atagaccggg aactggtccc gccgcttcca
      481 gatcagccat cccatcgtcg cgatgagcac cggccaatgg ccgtagatgt agaccgcatt
      541 caccgcccat acgagccagt cctgtcccag cgcccaacgc tgcatcccag gctcatggaa
      601 aataccgagc cgccgttcga gatccaccag ccagtgagcg tgctgcccgg caagcgccgc
      661 cgtgctgtcc atcatcccgc gcaccaggaa gtagaggagg acggcgagtg agacaatgcc
      721 gatctctcgc atcgggcctg aacgtgcggt cgtccggctg cgggacccgg aaattctttc
      781 ctcaccggag cggacagggc gggacacgac aacgtacggg ggcaatgtgc tcataggagc
      841 atcctttgac ggtcagacga cagagtccgc tgagtcccgt caacgtcgat ctcccctgcg
      901 ccgccaagcg tccatgcgct cgtccggggc gaccatcgtt gacgcatacc ggcaccatcc
      961 ggctgcatct ccatggtcat ccccggatga tcgtggagcg atcacgccac catcccatcc
     1021 cggggtcgtg gtttcctctc attaggggta gggttatccc tacgccatcc ctacatcgtg
     1081 cgtctgtacc accagccttt tcctcatccc ttgtcagtgg gacagattcc agggcgagcc
     1141 gggagacggc acctaccctg tggcaggggc cgaggtcgat ctggctgatc caggctctct
     1201 gcgccaaaga gccccctgtt tcagcgcaat accgaatgct taagggatga cggcatcagt
     1261 atttcagtcg tcacccagcc ctatcgatcc tgagactgag ggctcaggat cacgaccggg
     1321 atcactcggc tggtaccggc cgggtagccg atgtacatcc ggtatccccg aaacaatatc
     1381 ccggaggtga tcccgctcct cccctgtagc cgttctcggc cgcatctgtt gctgcttcag
     1441 atgcaggtgg ctgatgcccg cgggcgtata cgtctgcgtc cggcttcgtt gcttcggcgc
     1501 tggatcgccc agccagccag accaatgagc gccgagacgg cgaacccagc catgacgatc
     1561 caccagaacc agcccatggc ggtatccccc agccaggcaa acagcaacga ctgcgggatg
     1621 tagccgagga aggtcgccag cacataggtg cgtctccgca tattcgtcaa cccggccagg
     1681 taggagataa agtcggacgg agtgccggga atcgcgcgaa tcacaaagag ggccttgccg
     1741 ccccatcgat cgagccagcc ttcgaaggca cttccattga tccggccgat gagcttctcc
     1801 accgtgccct tgccaagtga tcgggcgatc ccgaaggcca gcattgcccc gccatctgtc
     1861 cagcgatgtt gagcagggtc ccccagagcg ggccgtaaac aacaccgttc gccagttgca
     1921 cgaccgacgc cgggatgggc gcaaagatca cctgtgccgt catcaggaag atggagacca
     1981 ccggtcccca ggcaccgtag ccgcgcaggt actcccggat ccgctggtgg tcaccgctgg
     2041 tgaggacatc gacgccttgg tgcacctctt cgcggaaagg attgctgagg agatacgtca
     2101 ccacggcgac gcccatcacc acgagcaacg tgatccacgg caggagggcg agccgatgcc
     2161 cccgccacca ggttcctgct gactcgtccc caaattcctg ctgaatcgtc tgcatgtgtt
     2221 ccgttatgtt cctgtcgtct tgtccgaacg cactgcccgt tgtgcgcccg gttaggatgc
     2281 ggacggttcc agacctgcaa tttgcgtcaa tgtcatcaca cgggtgcgca ggggccattg
     2341 cgcttctccg ctccagccca taggatgaag acccgttagt atcccgctct acgcattcta
     2401 ttcattgaca ctgcccgtgc gttcaattgc tcttcttacg cacaccaacc gacacgcacg
     2461 gaccggccat gcagcccgca tgggaaagcg tgggagatcc gctcatggct acaaccttct
     2521 gtatacgtaa cgtccaaccg gccgaaaacg atcccgtcga tgtcctcgtc cgggatggga
     2581 agattgcctc gatcggcgct ggcatcgttc cggacgaaga tgtggaggag atcgacggag
     2641 gcgggaggct cctcttcccc ggcttcgtcg atgcccatac ccacatggac aagaccctgc
     2701 tcgggttgcc ttggcaccgg cacacggcgg gccctgccct gctcgacaag atcgagggcg
     2761 agcgccgcct tcgtcaggaa gagggcatcg acgcgcacca gcagtcgacc cgtcacgccc
     2821 tgcaggcaat cgccagcggc acgacgcatg tccgttcctt cgcggatatc gacaccgaat
     2881 gggagctgaa gggtttccgt ggcctgatgc agacccgcga ggacctgagg gattcgctca
     2941 cgatccaggt cgtggccttt ccccagagtg gcatgctcgt ccgtcccgga acggtggagc
     3001 tgatgaagca ggctctcaag gaaggcgccg aggtcatcgg cgggctcgac ccgtcgacga
     3061 tcgatcggga tcccgccggt catctggaca ccatctttgg cctggccgag aaatacgatg
     3121 tcgatgtgga tatccatctc catgagccgg gcgagcttgg cgccttctcg atcgacctga
     3181 tcgccgagcg cacgcaggtg ctgggcatgc atgggcgggt tgtcatcagc catgcgttct
     3241 gtcttggcgg cgtcgacgat gcccggcttg acgggctcat cgacaaggtg ctgggaagcg
     3301 atatcgcgat catgagccat ggaccaagtg gcggcaggcc cgctcctccg gtcggacaac
     3361 tccgtgcggc cggggtgcgg atgtgcgccg gcaacgatgg gatccaggat gcgtggggag
     3421 cgctcaaccg gccggagatg ttgctccgtg cctatctcat cgcctatcgc aacaatttcc
     3481 gtcgcgatga cgagatcgag gatgtcatcg atatcatcac caatggtggc gcggatgtga
     3541 ttcgcgtggg cgactatggc ttcgccgagg gcaaggccgc ggatctcgtc cttgtcgaag
     3601 ccgagaatca cgtcgccgcg gtcgtcaccc aggcaacacc atggcttgtg atgaagcgtg
     3661 gaaaaatcgt cgcgcgcgat ggtgctatcg tgtaagggca cctggctact gccagattac
     3721 cggggcatgc acccatggag gtagaacgat gagcgtgact cctgaatcag accagggtac
     3781 caaggagcaa tccgtgccgc ccggcacccg gatcggccat gtccacctca aagtctccga
     3841 cctcgacagg gcagttcgct tctaccagga ggtgatggga ttcgatctca tcatccgcta
     3901 tcgcaaccag gcggcgttcc tctccgccgg cggatatcac caccatatcg gtctcaatac
     3961 atgggagagc ctgggcgggg caccgccgcc tccgggaacg accgggctct atcacttcgc
     4021 gatcaactac ccggaacggc gcgaccttgc ggcagcactg aagcgattgc tcgaccacgg
     4081 ctggggaatc gacggggcct cggaccacgg tacacacgag gcgctctacc tgcacgaccc
     4141 ggacatgaac ggcatcgagc ttgcctggga tcgcgatccg tcccactggt atcgcaacgg
     4201 cgaaccggtt ttcgagcgca atcccctcaa gttcgccagc cttctgggcg agttgggcga
     4261 cggcgaagcc tcgaagtatc tgaaagcctt ggcacgctac acctgatgca tccagccctc
     4321 tcggtggagg catgcaatgc cgcgggcgct ggtgagaaac gcgacgccgg atgatgccac
     4381 tgcgatcgcc gcgattcatg tcgcttcctg gcaggcgact taccgcgggc acatggacga
     4441 cgcctatctc gattcgctct ccgtggacga gcgggtggga atgtggtcgc agattctcga
     4501 atttccccgt acggctgcaa acgtcctggt cgccgagatc caaggcaaag tcatcggctt
     4561 ctattccttt gggcgatcgc gcgacagtga cctgcccggg gacaccgctg tactacacac
     4621 gatctatgtg cacccggacc tcataagatc aggcatcggt attgccctgt tggcgcgagc
     4681 ggagagggcc atgcgcgagg caggaattga cctcgtctcc ctctgggtac tccgggagaa
     4741 catcggtgcc cgccgcttct atgagcggtg tggacggtcc ttcaatggtg aagaaaggcg
     4801 cgaggaactg tgggggcagc ccccaacaga ggtgcgatac acccggtcgc tctcctctgg
     4861 tgaccaacgg gacatcggtg gcacctaact tggttgatgg attgaggacc gggcgcctgt
     4921 gctgccccga atccgcaagt tgaccaagaa atgttatgaa aggatgcctc cctcatggtt
     4981 cagatcgg
//
LOCUS       DASYWM010000060         5368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_639951, whole genome shotgun sequence.
ACCESSION   DASYWM010000060 DASYWM010000000
VERSION     DASYWM010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639951"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1909
                     /locus_tag="VGR29_02250"
     CDS             137..1909
                     /locus_tag="VGR29_02250"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEV2072440.1"
                     /translation="MKSGDGTTRHGIEQIRRRLREEAESISSGRNYQPSLDRRTFLLG
                     SLATGVSWTRMVSSQGTATPGASPMATPVASIELMENPFTLGIASGEPVPDGMVLWTR
                     LAPQPFEPHGGMPDASVELAYEIANDERFTDIVQQGPAIADAAWAHSVHLEVTGLEPD
                     RWYWYRFRIGEFDSLIGRTRTAPAPGASSEGLRFAFASCQRWEDGLFTAFRDMVQQDV
                     DLVIHLGDYIYEYTVRVIAELRDIEVPPTALSEIRTLDDYRMRYALYKLDPHLQEAHR
                     VAPWMVTWDDHEVSNNYIVPIIADDPFAKPLIERREAAYRAYYEHQPLRASATPKGPS
                     LRLYRAAEFGDLVRFNVLDERQYRTPGMGVCGAGEREELDGFCASAVDPDRTMLGEQQ
                     KQWLLDGFTQTTTRWNVLAQQVPMARIDYDPSAAGKSFGGEDLDKWDGYAYERDQVLA
                     AMAHAAKVQSFSPVVITGDVHANFVWNLKRDWDAPRGESIIGTEFVGTSIASNGDDPL
                     KEDGEFSTRCGNLRGNTHNLLFDNHRGYVLCDLTPDQWHTTYRVMPTVEDPDAIASTL
                     TSFVVEHDQPGAQMDQTCHAGGPA"
     gene            1913..3220
                     /locus_tag="VGR29_02255"
     CDS             1913..3220
                     /locus_tag="VGR29_02255"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HEV2072441.1"
                     /translation="MGFDRSGIEAALRRASTTRQVVVEPGAMARTGEILSSLTDGRTA
                     VAVADETTFGLAGDAVTSSLEASGIAVQPPVILSNVPVLHADYVQVAVVRSALEDAPA
                     GTIPVALGSGTLNDLTKRAAFEIGVPYIVVGTAASMDGYTASGASLVQDGFKTTLPCD
                     APIVVVADPDLLADAPVPMTASGYGDLAGKVTAGADWLLADTVGVEPIQPDIWAMVQH
                     PLHGALRDPGALRRRDVAAVEQLFLGLVMTGLAIQATGSSRSASGSEHQFSHLWEMRG
                     LEVSHGFKVAFGTLWSTHIYEAVLARSVQEIDWEGAVERWPDLEQQEGEVRAHIDDPV
                     MVTRALVEVRAKYVRKEALRARLATLRDEWAVLHQRLRAQLIPSSELRDLLQRAGCPV
                     TPEEIGLTPGQVRESVIAARQIRRRYTVLDLVLELGIRDEIVL"
     regulatory      3385..3492
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3834..>5368
                     /locus_tag="VGR29_02260"
     CDS             3834..>5368
                     /locus_tag="VGR29_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072442.1"
                     /translation="MTQTTYRDALISRRSFLQQVGVAGAAGALVAVGASVRAAAQDDP
                     ERREGGTIIYDMRQEGSHLVPAFSSFSTVIEPTVAFFNGLTRPGPELEPEPDLAESWE
                     VNEDGTHYVFHLREGATFHDGEPFTASDVKFTWELIAHPENVSAAQLYSFFSTITGAE
                     AFHAGEAEEIPGIKVVDDLTLDVTLDTTWAPFLTIGSGQYIVPKHILGEVPVGEILEH
                     EYARHPIGTGPFRFVAWQAGDSFIGEAFEDYYEGRPAADQLVMRIANLDDNAKITALR
                     SGELNGAQISLFNLPAIQGDPSVQIRQNPGRHNRYIEFNLAKPLFQDPRVRKALSYGL
                     NRQAIVDAVWQGQATIYNSPFPYSWEVTKQDTTMFDNDPEQAAQLLDEAGWTMGDDGI
                     REKDGEKFSFTMHTQSQDYPIVVQQQWKEIGVNAEVAYLDFPTLQTQFYTTGEFDAVA
                     IWVPYSLYPDPHYALPGYFLSANNRNSYNNPESDKLIIAAASTIDPEERKRLYYEWQE
                     VIA"
BASE COUNT         1043 a         1575 c         1738 g         1012 t
ORIGIN      
        1 acgtttcttg cctttaaggt gcacggtcca tcttggattt cgaacgggag cattcttcaa
       61 accaatgcca agcggaagat gcacgggcgc caaaccgggg tatcatggcc attcatcgtg
      121 aagaggggaa accatcatga agtccggcga cggcactacg cggcatggca tcgagcagat
      181 acggaggcgg ctgagggagg aggcagagtc gatctcatcc ggccgtaact accagccctc
      241 actggatcga cggacgttcc tgcttggatc gctggcaacc ggcgtcagtt ggacacgaat
      301 ggtttcctcc cagggaacgg ctacaccggg tgcttctccc atggcaacgc cggtcgccag
      361 tatcgagctg atggaaaacc cgttcaccct cggcattgca tcgggcgaac cggttcctga
      421 tggcatggtc ctgtggacgc gcctggcacc gcagccgttc gaaccgcatg gcgggatgcc
      481 cgatgcctcg gtcgaactcg cctacgagat cgccaacgat gagcggttca ccgacatcgt
      541 gcagcagggc ccggcgatcg ccgatgccgc gtgggcacat tcagtgcatc tggaagttac
      601 ggggctcgag ccggaccggt ggtactggta ccggttccgg atcggggagt tcgacagtct
      661 aatcggccga acacgcaccg ccccggctcc tggtgcctcc tctgaggggc tccggtttgc
      721 gtttgcgtcc tgccaacgat gggaggacgg gctcttcacc gccttccggg acatggtcca
      781 gcaggatgtc gatcttgtga tccacctagg ggactacatc tacgagtaca ccgtgcgtgt
      841 cattgctgaa ctccgggata tcgaggtgcc gccaaccgcg ttgagcgaga tccggacatt
      901 ggacgactac cggatgcggt atgcgcttta caagctcgat ccgcatctgc aggaggcgca
      961 ccgggtggcg ccctggatgg tgacatggga cgaccatgag gtgagcaaca attacattgt
     1021 gccgatcatt gccgatgatc cttttgccaa accgctgatc gagcggcggg aggcggccta
     1081 ccgggcctac tacgagcacc agccactgcg ggcatcggcc acgccaaagg gcccgagcct
     1141 tagactctac cgggcggcgg agttcgggga cctggtgcgg ttcaacgtgc tggacgagcg
     1201 gcaataccgg acgcccggga tgggtgtctg cggcgctggc gaacgagagg aactcgatgg
     1261 cttctgcgcg tcggcggtcg accctgatcg gacgatgctt ggcgagcagc agaagcagtg
     1321 gctcctggac gggttcacgc agacgaccac ccgctggaac gtcctcgcgc agcaggtccc
     1381 gatggcgcgg atcgactatg acccttccgc cgccggcaag agcttcggtg gtgaggatct
     1441 ggacaagtgg gacggctatg catacgagcg cgaccaggtg ctggctgcga tggcccatgc
     1501 ggccaaggtg caatccttca gcccggtcgt gattacgggc gatgtgcacg ccaattttgt
     1561 ctggaacctg aagcgcgact gggatgcgcc gaggggcgag agcatcattg gtacggagtt
     1621 cgtgggtaca tcgatcgcct ccaacggcga tgatcccctc aaggaagatg gcgagttctc
     1681 gacccgttgc ggcaacctgc gtggcaacac gcacaacctt ctcttcgaca accaccgtgg
     1741 ctacgtgctc tgcgacctga caccggatca atggcatacc acctatcggg tcatgccaac
     1801 agtggaggat ccggacgcga tcgcgagcac gctgacctca tttgtggtcg agcacgatca
     1861 gccgggcgcg cagatggatc aaacatgcca tgcaggagga cccgcatagg ccgtgggatt
     1921 cgaccggagc ggtatcgagg cggcgttgcg gcgagcatcg accacgcggc aggtggtcgt
     1981 cgagccgggg gcaatggcgc gcacgggcga gatcctctcg tcgctcactg acggccggac
     2041 cgccgtggcc gtcgctgacg agacgacgtt cggtcttgcc ggagacgcgg tgacctcttc
     2101 gctggaggcg tctggaatag ctgtgcagcc gccggtgatc ctgagcaatg tgccggtgct
     2161 ccatgccgac tatgtacagg tggcagtcgt ccggtcggcg ctggaggatg cccctgcagg
     2221 aacgatcccg gtggcgctcg gttccgggac actgaacgat ctgacgaagc gggctgcctt
     2281 tgagatcggt gtgccctaca tcgtggtggg gactgccgct tccatggatg gctacaccgc
     2341 atccggggca tcgctggttc aagacggatt caagacgaca ttgccgtgcg atgcaccgat
     2401 tgtcgtagtc gcggatcctg acctgcttgc ggacgcgccg gtcccgatga cggcatccgg
     2461 ttacggggat ctggccggta aggtcacggc cggggccgac tggctgctcg ccgatacggt
     2521 cggggttgag ccgatacagc cggacatctg ggcgatggtg cagcatccgc tgcatggcgc
     2581 gttacgcgac ccgggcgccc tgcgccgtcg ggatgtggcg gcggtcgagc aactcttcct
     2641 cggtctggtc atgacgggcc tcgcgatcca ggcgaccggg tcatcgcgtt cagcttcggg
     2701 aagcgagcat cagttcagcc acctgtggga gatgcggggc ctggaggttt cgcatgggtt
     2761 caaggttgcg tttggcacgc tctggtccac gcatatctat gaggcggtcc ttgcgcgaag
     2821 cgtgcaggag atcgactggg agggcgcggt tgagcgttgg ccggatctgg aacagcagga
     2881 gggggaggtc cgcgcccata tcgatgatcc ggtcatggtc accagggcgc tcgtggaagt
     2941 ccgggccaag tacgtgcgga aggaggccct gagggcgaga cttgcgacgc ttcgggatga
     3001 gtgggctgtg ctccaccaga gactgcgggc gcaattgatc ccgtccagtg agctgcggga
     3061 cctgctgcag cgggccggtt gccccgtgac gccggaggag atcggcctca cccccggtca
     3121 ggtgcgggag agcgtgatcg ccgcgcggca gatccggcgg cggtacacgg tgctggacct
     3181 agtgctggag ctggggatcc gtgacgagat agtcctgtag tcccgaagtg gtggaagcca
     3241 gaaggcattg tgcccaacaa cgggagcccg gattgatgcc gggaaggacc tctgtcctgc
     3301 ccattttgac accaaccata cggtgctgtt gacacggaag tggctaatac atagtatagt
     3361 ggccaacaat tgaatacaag cacgatctca tccagagggg tagagggaac ggcccgatga
     3421 aaccccagca accatttggc ggtaacgccg aaaaggtgct aattccgaca ggatgttcct
     3481 gggagatgag ggagtgatca gtatcctcaa cgggataccg cccaccccct gatctccgag
     3541 atgggggttt gttatgggtc atcggcacgg gtcaataccc agtacgtcca cgacgcgcat
     3601 tcaggacaac gggatcagtt tatgtcccgc gaggagcgcg gtcattccaa taacgtctat
     3661 ggtgccatgc cccagcgttg tcccagtata tgtagtatgt gctgagatcc cctccgccct
     3721 gtgatcacac aggggcccct ccggggcaca tacattcctg ttttttgttg cttgtcagcg
     3781 aacgagcgga tttctctctt catcattcat cagcttttca ggagacagtg actgtgacac
     3841 agaccacgta tcgggatgca ctcatttctc gccggtcctt ccttcagcag gtgggcgtcg
     3901 ccggagccgc gggggcgctg gtggccgtcg gggcatctgt acgggcggct gcacaagacg
     3961 acccggagcg ccgcgagggc ggcacgatta tttatgacat gcggcaggaa ggctctcacc
     4021 ttgtgccggc gttcagctca ttctcgaccg tgatcgagcc gacggttgcg tttttcaatg
     4081 ggctgacgcg gccagggccc gagctggagc cagagccgga cctggcggag tcgtgggagg
     4141 tcaatgaaga cggcacgcac tacgtcttcc acctgcgtga gggcgcgacc tttcacgacg
     4201 gcgagccatt cacggcaagc gatgtgaagt tcacctggga gctgatcgcg catccggaga
     4261 acgtctcggc ggcgcagctc tacagcttct tctccacgat caccggggca gaagcgttcc
     4321 atgccggtga agcggaagag atccccggga tcaaggtcgt cgatgacctc acactcgacg
     4381 tgacgctgga tacgacgtgg gcgcccttcc ttactatcgg ctccggccag tacattgtgc
     4441 cgaagcacat cctcggcgag gtgcccgttg gagagattct ggagcacgag tacgcacgcc
     4501 acccaattgg taccggcccc ttccggtttg tcgcatggca ggcgggggac agctttatcg
     4561 gtgaggcatt cgaggactac tacgaagggc gcccggccgc agaccaactc gtgatgcgta
     4621 tcgcgaatct ggatgacaat gccaaaatca ctgcccttcg ctccggcgag ctcaatgggg
     4681 cccagatctc gctgttcaat ctccccgcta tccagggcga tcccagcgtt cagattcggc
     4741 agaaccccgg tcgtcataac cggtacatcg agttcaatct cgcgaaaccg ctcttccagg
     4801 accctcgtgt ccgcaaggca ctgtcatacg gactgaaccg tcaggcaatc gttgatgccg
     4861 tctggcaggg ccaggcgacc atctacaact ctccgttccc ctattcctgg gaggtcacca
     4921 agcaggacac caccatgttc gacaacgatc cggagcaggc cgcgcaactc ctggacgagg
     4981 cggggtggac gatgggagac gatggcatcc gcgagaagga tggggagaag ttctccttca
     5041 ccatgcacac ccaaagccag gattatccga tcgttgtgca gcaacagtgg aaggagatcg
     5101 gggtcaacgc ggaggtggcc taccttgatt ttccaacact tcagacgcag ttctatacga
     5161 ccggtgagtt cgatgccgtc gcgatttggg tgccgtacag cctctatccc gacccgcact
     5221 acgcacttcc cgggtacttc ctgagtgcaa acaaccggaa cagctacaac aatccggagt
     5281 ccgacaagct gatcatcgcc gcggcgtcga ccattgaccc ggaggagcgc aaacgcctct
     5341 attacgagtg gcaggaggtc atcgcgca
//
LOCUS       DASYWM010000061         6956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_377267, whole genome shotgun sequence.
ACCESSION   DASYWM010000061 DASYWM010000000
VERSION     DASYWM010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377267"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VGR29_02265"
     CDS             <1..370
                     /locus_tag="VGR29_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072443.1"
                     /translation="MQQKIALARALLTSPMLMLLDEPTTGLDPRSKKDVQALIRSIRK
                     DHASSILLCTHDMGEAEELCDRIGILLNGELVAIDTADGLRQKYGTSSLEDVFMAATG
                     TSLEEAEFAAEPVENASAAY"
     gene            380..1222
                     /locus_tag="VGR29_02270"
     CDS             380..1222
                     /locus_tag="VGR29_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072444.1"
                     /translation="MEITHTELSKPSWMRETIAIWGYAQRNYFLTKRYFWWEVVWLVY
                     STMNALTIGFIGVGFGGTAEEAARATTFLLIGTLLWSYLSMIFDLLSETVSWERWEGT
                     IEYTFMSPASRTSQLLGMGIYAVLYGIVRMIIMLGAISLFFDMSLGDANYAAALGILA
                     ICSVSLVGFGMVAAVMPLLSPEKGQQVTSIFTAVLLLISGIYYSIDVLPGWMQPLANI
                     SPVYYALNGIRAALLEGASFTSQWGNIWPLLVMGGIFPPLGLWMFQKGEWYAKKTGRL
                     KRSG"
     gene            complement(1370..2050)
                     /locus_tag="VGR29_02275"
     CDS             complement(1370..2050)
                     /locus_tag="VGR29_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072445.1"
                     /translation="MHPAPTLLRGGPGHDLLRRVMRIRTEQAPIERIILIDEPDLRLT
                     LRDTGPEGTPVATINRETALHILWALDDESALVHIEEQEIRIAPGDTMLLPTGTPWRA
                     SPRMILCEIAGSRFDEPSESDHPSIIDPTHGQETFHAYNRQTTYPSPHGLEIERWKIT
                     QPLLLPDSPSSYVIVDLVNPLALVWPGGTDLIGRGESRVILPCTGAVTLIPDGLGYAL
                     IIRHALCG"
     gene            complement(2062..2385)
                     /locus_tag="VGR29_02280"
     CDS             complement(2062..2385)
                     /locus_tag="VGR29_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072446.1"
                     /translation="MTIDAGTTALKIMQFGSDLHLQSTDCIPNSGGAIIGLTPEASPG
                     ELTRAALEGIATGTTVPARVSHRIRPDPGMVNRYHERYEIYLEALQAVTPISHRRASL
                     GNHRP"
     gene            complement(2388..3206)
                     /locus_tag="VGR29_02285"
     CDS             complement(2388..3206)
                     /locus_tag="VGR29_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072447.1"
                     /translation="MAIPTTVLLVWNQVLSSLHVLRDRTAQACALTSLIVTLIAIATV
                     PDIVPGFWDWFFLLLIGALSMVAIITFVASRQASVAPADRQHRVTAAFTIGVIALVLN
                     IFFELSSTILLGIGMVMLARQAVRVRPGTFPWLLCATLVILIPWWVWSALDAWHPGLF
                     LLIPLAALAYLTGSHIREAYATTDDAPRPLSTRGHRLGAWMGMLLGGILIVIGGLIGS
                     SSYGWISLGGIAMAVAVAVEAGISRPEDQPGKYSATICDGAFVIGGLCWLISIT"
     gene            complement(3285..3695)
                     /locus_tag="VGR29_02290"
     CDS             complement(3285..3695)
                     /locus_tag="VGR29_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072448.1"
                     /translation="MIPDTVVAIVSEESLPDLLTQVHRSGFGQNARVLRPRRSPLREQ
                     LRRAGISIEQAPERLDAAACLLTILAAGRSPAAADLALHHGASATWIVTRSGAWNLVD
                     DRILPDATGQKGGQAIPAPVAVLQEEPSDVSGSI"
     gene            complement(3979..5388)
                     /gene="mgtE"
                     /locus_tag="VGR29_02295"
     CDS             complement(3979..5388)
                     /gene="mgtE"
                     /locus_tag="VGR29_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEV2072449.1"
                     /translation="MTSTLTDTDPTPTPNDVSNDDTLLEDIRAAVANKDVDRARSLAN
                     RMLEEDALALLEDLTPIELSRLFTFLGDETLALLLSRLDDRDAAGILTRMSAAQAADI
                     LEQIDPDDATDIFTEVENSDPSAAGTIMIEMDPDEAAEIRDLMAYGADTAGGIMTPAF
                     VAVFPELRADETVTALRKVAAEAETINYVYVVDRDERLLGVLSLHSLVLSRPETPVRE
                     LMATDTWSIAVDEDQEVAARIMTERDLLSLPVVDHDGRLLGIITHDDIADIIEEEATE
                     DIERLGGSQPLEVPYLRASPFLLFRKRIVWLLVLFVAQIFTVSIQEHYDVVLQQVTLL
                     AVFIPILIGTGGNVGSQTVSTIIRAMAVEEVGPRHVLRIIGKEALTGLSLGVVMGCLM
                     FGRALLTGDGSTEIALTVGLTIMALTTWAATIGAILPIALSRLKFDPAVVSAPFISSF
                     VDGTGLIIYFTLAQFLLQL"
     gene            5581..5793
                     /locus_tag="VGR29_02300"
     CDS             5581..5793
                     /locus_tag="VGR29_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072450.1"
                     /translation="MIGHGGGVLRLDLMGRRTARSLDRHLADDGLALLVVVVDVETTW
                     RIGLGHAALPFTPERTTRWVVETIYG"
     gene            6150..>6956
                     /locus_tag="VGR29_02305"
     CDS             6150..>6956
                     /locus_tag="VGR29_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEV2072451.1"
                     /translation="MTGTATDTWASEPRFDQERLGAFVKQLFVAAGVGADDAAQATDA
                     LLLADLRGVESHGVARLPGYVGRLRAGLIDADAELTVERETPSTIAFNANNGLGLTVG
                     SRAMSRCIAKAEETGICMATVRRSNHFGIAGSYALMAAEQGFAGMAMTNSSAIVVPMY
                     GKEPMLGTNPLAFAVPTGSLPFCLDMSTSTVAFGKIEVARRAGLPIPAGWGVDSDGRP
                     TTDADAVRGLTPLGGPRETSGHKGYGLGLMVEIFCGQLASNTWSHQIGASY"
BASE COUNT         1390 a         1952 c         2306 g         1308 t
ORIGIN      
        1 tatgcagcaa aagatcgcgc tggcgcgagc gttgctcacc agcccgatgc tgatgctgct
       61 tgacgagccg acaaccggcc tggatccgcg gtccaagaag gacgtgcagg cgttgatccg
      121 ctcaatccgc aaggatcacg cctcatcgat cctgctctgc acgcacgaca tgggtgaggc
      181 ggaggagctc tgcgaccgga tcggaatcct gctgaacggc gagctggtcg cgatcgatac
      241 cgctgacggc ttgcggcaaa agtacgggac ctcctccctc gaggatgtct tcatggcggc
      301 taccggaaca agtttggagg aggcggagtt cgccgctgaa ccggtcgaga atgcatcggc
      361 agcttactag ggaaggacga tggagattac gcacacggaa ctgagcaaac caagctggat
      421 gcgcgagacg atcgcgatct gggggtatgc ccagcggaac tactttttga ccaagcgcta
      481 cttctggtgg gaggtagttt ggttggtcta ttcaacgatg aacgcgctga caatcggctt
      541 catcggggtc ggtttcggcg ggaccgccga agaggcggcg cgggcaacga ctttcctgct
      601 catcggcacc ctgctttgga gctacctgtc gatgatcttc gacctgctct cggagacggt
      661 gagctgggag cgatgggagg gcacgatcga gtacaccttc atgtcgccgg cgagccgcac
      721 ttcgcagctc ctcggtatgg gaatctacgc ggtgctctac ggtatcgtgc ggatgatcat
      781 catgttaggc gcgatctcgc tcttctttga catgtcactg ggggatgcca actacgcggc
      841 tgcgctgggg atcctggcga tctgtagcgt ctcgctggtg ggattcggca tggtggcggc
      901 cgtcatgcca ctgctgtcac cggagaaggg tcagcaggtg acaagcatct tcaccgccgt
      961 gctgttactg attagcggca tctattacag catcgacgtg ctgccggggt ggatgcagcc
     1021 gctggcgaac atctctccgg tctactatgc gctgaacggg attcgtgccg cactgctgga
     1081 gggcgcttcg ttcaccagcc agtggggcaa tatctggcca ctgcttgtga tgggcggcat
     1141 cttcccaccg ctgggcctgt ggatgttcca gaagggcgag tggtacgcga agaagacagg
     1201 ccgcctgaag cgaagcggat gatgcggaag aaggggtcga cacttgcgcc ggccccttcg
     1261 cgcttggtac ggcgtcatgc ggctctgact gacatgcggg ctgcctccaa ccagcccgtt
     1321 gaccacgggt taagagcggc tatggcccgt cgggcacggg tctcatatct cagccacaca
     1381 gggcgtgccg aatgatcaac gcatacccca atccgtccgg tatgagcgtc accgcgccgg
     1441 tgcagggcag aatgacacgg gactcgccgc gaccgatgag gtcggttccg cctggccaca
     1501 cgagggcaag cggatttacc agatcgacga tgacatagga agagggggaa tccggcaaca
     1561 acagcggctg ggtgatcttc cagcgctcga tctccaaacc gtggggagac gggtatgttg
     1621 tctgacggtt gtaggcgtgg aaggtttcct ggccgtgcgt cgggtcgata atgctgggat
     1681 gatcggactc gctgggttca tcaaacctgg aacccgcgat ctcgcagagg atcatccggg
     1741 gagatgcccg ccatggtgtg ccggtgggca ggagcatcgt atcgcccgga gcaatcctga
     1801 tctcttgctc ctcgatgtga accagggcgg attcatcatc caacgcccaa agaatgtgaa
     1861 gcgctgtctc acgatttatt gttgctaccg gcgtcccctc cgggccggta tcgcgcagtg
     1921 tcaatctgag gtccggttcg tcgatcagga tgatccgctc gatcggggcc tgttcagtac
     1981 ggatgcgcat cacccggcgc aaaaggtcgt gtcccgggcc gccgcgaagg agcgttgggg
     2041 cggggtgcac cggcgcgggt ctcatggccg gtggtttccc agcgatgcac gtcgatggga
     2101 gatgggagtg accgcctgga gtgcttcaag atagatctcg taacgctcgt ggtaccggtt
     2161 gaccatgcca ggatcaggtc tgatgcgatg actgacccgt gcgggtaccg ttgtgcctgt
     2221 ggcgatgccc tccagcgctg cgcgggtgag ttctcccgga gacgcctccg gtgtgaggcc
     2281 gatgattgca ccacccgagt tcgggataca gtctgtcgat tggagatgca gatcactgcc
     2341 gaattgcata atcttgagcg cggttgtgcc cgcatcgatt gtcatggtca ggtaatgctg
     2401 atgagccagc agagcccacc gatgacgaaa gcgccatcgc agatcgttgc ggagtatttg
     2461 ccgggctggt cttctggccg ggagatcccg gcctcgaccg caaccgcaac cgccattgcg
     2521 atgcctccga gtgaaatcca cccgtaggat gaagagccga taagcccacc gatgacgatg
     2581 aggatacccc ccagcaacat tcccatccat gcaccgagac gatgtccccg tgtagagagc
     2641 ggtcggggcg catcatcggt cgtggcatag gcttcacgga tgtggctacc ggtcaggtag
     2701 gccagggctg caagcgggat cagtaggaag agacccgggt gccaggcgtc caacgcggac
     2761 cagacccacc atggaatgag gatcacgagg gtcgcgcaga gtagccacgg gaaggttccc
     2821 ggtcggacgc gaacggcctg acgggccagc atcaccatgc caatgccgag caagatcgtc
     2881 gacgagagtt cgaaaaagat gttgaggacc agggcaataa cgccaatggt gaacgcagcg
     2941 gtgacgcgat gctgccggtc agcgggggct acactagcct ggcggctggc aacgaaggtg
     3001 atgattgcca ccatcgacag ggcaccgatc aggagcagga agaaccaatc ccaaaatccc
     3061 ggaacaatat ccgggacagt cgcgatggcg atgagcgtca cgatgagcga ggtcagcgca
     3121 caggcctgtg ccgtgcggtc gcgcaacacg tggagtgagg aaagaacctg gttccagacg
     3181 agaagaaccg ttgtaggtat ggccacgctg cgcctcgcag tgaatccgac agcaatcgag
     3241 agatgatgtg acccttttgg gtcgccgcct cagtagcgaa tgtatcagat cgacccgctc
     3301 acgtcggaag gttcctcctg aagcaccgcg accggcgcgg ggatcgcctg ccctcctttc
     3361 tgacctgttg cgtccggcag gatacggtcg tcaaccagat tccaggcgcc tgagcgggtg
     3421 acgatccacg tcgcggacgc accatgatgc agggcgagat cggccgcggc cggacttcga
     3481 ccggccgcca ggattgtgag gaggcaagcg gcagcatcca ggcgctccgg ggcctgctca
     3541 atggaaatac cggcacggcg gagctgctct cgaagagggg agcgtcgtgg ccgcaggacg
     3601 cgggcattct gaccaaaacc ggaccggtga acctgggtaa gcagatcagg aagcgattct
     3661 tccgaaacga tggcgacaac agtgtcggga atcatgggtt gcggggcctt gggttacgaa
     3721 cgtggaaatg caatagcgtg accatcagtc acagtgtcat gaatcgtagc actttggctg
     3781 gtgcggccgt ggccgcggag tcatggtcag acccctccgc gaggatactg tccctggcgc
     3841 ctgaatacag atggtgctct agcaaaaggt tcatgctgcg tggttgcgga gctatcaacg
     3901 gatcgcaccc acgatgcgtt caggattgtg cctgactgag gtccactgca actcgacgcg
     3961 tttcccgtgc aacgtgcctc aaagctggag caggaactgc gcaagggtga agtagatgat
     4021 cagtccggtg ccgtcgacga aactgctgat gaacggggcg gagacgaccg ccgggtcgaa
     4081 cttcaaccgg ctgagtgcga tcggcaggat cgcgccgatg gttgccgccc aggtcgtaag
     4141 tgccatgatg gtcaggccga ccgtaagggc gatctccgtg ctgccatcac cggtcagcag
     4201 ggcacggccg aacatcaggc accccatcac cacgcccagg gagagaccgg tcaaggcctc
     4261 cttgccgatg attcgcaaga catgcctggg accgacctcc tcaaccgcca tggcgcggat
     4321 gatcgtggag acggtctggg agccaacatt accgccggtg ccaatcagga tcgggatgaa
     4381 gacggcaaga agcgttactt gctgcagaac gacatcgtag tgttcctgga ttgacacggt
     4441 gaaaatctgc gccacgaaca ggacgagcag ccacacgatg cgcttgcgga agagcaggaa
     4501 agggctcgcg cgaaggtaag gaacttccag tggctgcgat ccaccaagcc gctcaatgtc
     4561 ctcggttgct tcttcctcga tgatgtcggc gatatcgtcg tgggtgatga taccgaggag
     4621 cctgccgtca tgatcgacga ccggaagcga cagcaggtcg cgctcggtca tgatcctcgc
     4681 cgcgacttcc tggtcctcgt caaccgcgat agaccaggta tcggtggcca tcagctcgcg
     4741 gaccggggtc tcgggacggc tcaagacgag gctgtgtagt gagagcactc cgaggagcct
     4801 ctcatcacgg tctacgacgt agacgtaatt gatcgtctct gcctctgccg cgaccttgcg
     4861 gagcgcggta acagtctcgt cagcacgcag ctccgggaag accgcgacaa aggctggagt
     4921 catgatgccg ccggcggtat cagcgccgta ggccatgagg tcccggatct cggctgcctc
     4981 atccgggtcc atctcgatca tgatggtccc ggcggcgctc gggtcggagt tttcaacctc
     5041 ggtgaagatg tcggttgcgt cgtcgggatc gatctgctcg agaatatctg ctgcctgggc
     5101 ggcgctcatc cgggtcagga tgccggcggc atcgcgatcg tcgaggcgcg acagcaacag
     5161 ggcgagcgtc tcatccccaa ggaacgtaaa gagccggctg agctcgattg gcgtcaaatc
     5221 ctcaaggagg gcgagcgcat cttcctcgag catccgattc gccagggagc gggcgcggtc
     5281 gacatccttg ttggctaccg cagcgcggat atcctccagc aaggtgtcat cgttgctgac
     5341 atcatttgga gtcggtgttg gatcggtgtc agtgagcgtg ctcgtcatgc gacggaacgg
     5401 tcctgtcttc ccggattctg catctgcgcc agcccttcct gaagttgaat tcgcacacgc
     5461 cggagcgccc gcgtcacatc agtgagcggc gctccgatat tgcgtgcgtg tgtgctatgt
     5521 ggccctacgc cgcatggcag attgcatgag cttgctgata cgtggattac ggcgaacctg
     5581 atgataggcc acggcggggg ggtcttgcgg ctggacttga tgggtcgccg gactgctaga
     5641 tcgctggacc gacaccttgc ggacgatggg ctcgccctgc tggtcgtcgt ggtcgatgta
     5701 gaaacgacgt ggcgaattgg ccttggtcat gcggcactgc ctttcacgcc tgaacgcacc
     5761 acgcgatggg tcgtggagac catttatggg tgagagcggg cgattgtccc cggtgcgacc
     5821 ggggcagcgt gccaatcagg ggcaggcgct ggccggtcta gttccggagg aaatccattg
     5881 aactgaaacg cggactgtca gcgtccaacg aggctccctt cacgggccaa aacggggaga
     5941 gtggctcaac ccgcaccaga acgacgacat cccatagatg ggacgccacc atcgatgatg
     6001 ctactccacc gcaaatcagc cgtcaagacg cgagattcgg ttcatttgat gcaagctcgg
     6061 caagcaggac gccgcccgag ggattgccag cagacgaggt gggttggaca atcgatgccg
     6121 gtgaacagag ggctcttgga ggacgaggga tgacgggaac agcgactgat acatgggcgt
     6181 ccgaaccgcg tttcgaccag gagcggctcg gtgcattcgt caagcagctt ttcgtggcgg
     6241 ccggtgttgg ggcagatgac gccgcacagg cgacagatgc gcttctgctt gcagatctgc
     6301 gtggagttga atcgcatggg gtcgcccgtt taccagggta tgtcgggcgg ctgcgggcag
     6361 gattgattga cgccgatgcg gaactgacgg ttgagcggga gacgccgtct actatcgcgt
     6421 tcaacgcgaa caacgggctg ggtctcaccg tgggcagtcg ggcaatgtcg cgctgcatcg
     6481 cgaaagcaga ggaaaccggt atctgcatgg caactgtccg gcggagcaac cacttcggca
     6541 tcgcagggag ttacgccttg atggcggccg agcagggatt cgccggtatg gcgatgacca
     6601 actcttcggc gatcgtggtg ccgatgtatg gcaaggagcc gatgctgggg acgaatccgc
     6661 tggccttcgc cgtgccgacc ggtagcctgc ctttctgcct cgacatgtca acctcgaccg
     6721 tcgccttcgg gaagatcgag gttgcccggc gggcgggtct tcccattccg gcggggtggg
     6781 gtgttgacag tgacgggaga ccgacaacgg atgccgatgc cgtccggggg ctgacgcctc
     6841 ttggcggtcc aagagagaca tccgggcaca agggatatgg gcttgggttg atggtggaga
     6901 tcttttgcgg ccagctggcg agcaatacct ggagccacca gatcggtgca tcctac
//
LOCUS       DASYWM010000062         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_133256, whole genome shotgun sequence.
ACCESSION   DASYWM010000062 DASYWM010000000
VERSION     DASYWM010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133256"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..595)
                     /locus_tag="VGR29_02310"
     CDS             complement(89..595)
                     /locus_tag="VGR29_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072452.1"
                     /translation="MAPCVSRSWMVLVLFGASGTGKSAAAAEIGCRYGVSWVQVDDLR
                     LTLQYSRVTLPERTDELYFLEGSSGVWTRTPAELRQAFINVAELMAPALRTVIHSHVV
                     TGAPMVIGGDGVSPALAVDPLLKPLVDTGVIRFCCTATPDVEEVVARAVFRRNTRRGD
                     RSGVGPQR"
     gene            complement(607..1143)
                     /locus_tag="VGR29_02315"
     CDS             complement(607..1143)
                     /locus_tag="VGR29_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072453.1"
                     /translation="MADSDRTTTHAAKEAFPEEEMRPLPDGGLESGMPEWLRRPPAWR
                     NLPREGSGEERSLPEPDTSVIDPRTLVDVADLPRWLQEIAARHGETTDVTKPVTTKQI
                     NGDGAMQTNDQNNSDTPNTNERQVPFEPVDKKKWTIPEKETKVYGGGPPKGSNTMMLL
                     AIGIVALIVILIILFVLL"
     gene            complement(1148..1630)
                     /gene="dtd"
                     /locus_tag="VGR29_02320"
     CDS             complement(1148..1630)
                     /gene="dtd"
                     /locus_tag="VGR29_02320"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEV2072454.1"
                     /translation="MRVCLQRVSRSSVTVAGEVVSSIGAGYLILLGVTVGDGGDEVEM
                     LTSKIANLRILDGEHGIMNRSALEYMAADPESVAMLIVSQFTLYGDVRKGRRPSWIDA
                     APPAVAKPMVETFCKAMGNLGFHVEQGVFGAEMAVELINDGPVTMWLDSDELRKPRRG
                     "
     gene            complement(1627..>2031)
                     /gene="ispF"
                     /locus_tag="VGR29_02325"
     CDS             complement(1627..>2031)
                     /gene="ispF"
                     /locus_tag="VGR29_02325"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEV2072455.1"
                     /translation="YGRGLDGHSDADVLLHAIADALLGAAALGDIGVHFPPTEAKWKG
                     MDSRDILRHAVALLRKHGWEPVNLDATVIAEAPRVNPHVPAMREEISRVSGLGEDAIS
                     IKATTNEGLGFVGRGEGIASLASATVMRTGAR"
BASE COUNT          390 a          684 c          556 g          401 t
ORIGIN      
        1 ttcgccaagc tcaaacacta tctgcgggcg gtcaaggccc ggaccgtcga catggtgatg
       61 gcggcaatcg agcacctcta tccccggatc accgctgcgg acccacgcca cttctatcac
      121 cacgccgggt atttcttctg aaaaccgctc tagccaccac ctcttcgaca tccggtgttg
      181 cggtgcaaca gaagcggatg acgccggtat cgacgagcgg tttcagcaga ggatccacgg
      241 ccagcgcggg cgagacgccg tcacccccga taaccattgg ggccccggtc accacatggc
      301 tatggatcac ggtgcgtaga gccggagcca tcagctccgc cacgttgatg aacgcctgcc
      361 ggagctcggc aggcgttcgc gtccagacac cggaagaccc ttcaaggaag taaagctcgt
      421 ctgtgcgttc aggtagggtc acccggctgt actgcaatgt aaggcgcaga tcgtcgacct
      481 gaacccagga gactccatat cggcatccga tctccgcggc ggccgccgac tttcccgtgc
      541 cgctggcgcc gaagagcacc agcaccatcc atgagcgtga tacgcacggc gccacgcagc
      601 ctcctgttac agaaggacga agaggatgat caggatcacg atgagagcga caatgccgat
      661 ggcaaggagc atcatcgtat tgctgccctt gggcggtcct ccaccgtaga ccttggtttc
      721 cttttcagga atggtccact tcttcttgtc gaccggctcg aatggcacct gccgctcgtt
      781 cgtgttcggc gtgtcggagt tattctggtc gttggtctgc atcgctccat ccccattaat
      841 ctgctttgtt gtgacgggct tagtaacgtc ggtggtttct ccgtgacggg cagcgatctc
      901 ctgcaaccag cgcgggagat ctgccacatc gacgagtgtt cgcggatcga tgacggacgt
      961 gtccggctcc ggcaatgagc gctcctcccc ggagccctcg cgaggcaggt ttcgccaggc
     1021 aggaggacgt ctcaaccact cgggcatccc gctctccaga ccgccgtccg gcagcggccg
     1081 catctcctcc tccgggaatg cctccttcgc cgcgtgagtg gtcgtcctgt cgctgtcggc
     1141 cacgctgcta tcctcgccgg ggttttcgaa gctcatcgct atcgagccac atcgtgaccg
     1201 gcccgtcgtt gataagctct accgccattt ccgcgccaaa gactccctgc tccacatgga
     1261 atcccaagtt acccatggcc ttgcaaaacg tctcgaccat cggttttgcc acagccggcg
     1321 gggcggcgtc gatccagctg ggtcgtctcc ctttccggac atcgccatag agtgtaaatt
     1381 gtgagacgat gagcatcgcg acactctctg gatcggccgc catatactcg agcgcggacc
     1441 gattcataat gccatgttca ccgtccagaa tgcgcaggtt cgcgatcttg ctggtcagca
     1501 tctcaacttc gtcgcccccg tcccccaccg tcacaccgag caggatcagg tatccggcac
     1561 cgatgctcga cacaacctca ccggccaccg tcacactgga gcgggaaacc cgctgcaggc
     1621 acaccctcat cgcgcaccgg ttcgcatgac ggtcgcgctc gccagcgagg cgataccctc
     1681 accccgtccc acgaatccca agccttcgtt cgtagtcgcc ttgatgctga ttgcatcctc
     1741 cccgagccca gagacgcgag aaatctcttc acgcatcgcg gggacatgag ggttgaccct
     1801 tggcgcctcc gctatgacag tggcatcgag gttcactggt tcccagccgt gcttcctcaa
     1861 caacgcgact gcatggcgca ggatgtccct gctatccata cccttccact tcgcctcggt
     1921 gggaggaaaa tgcacgccga tatcgccaag cgcagccgcg cccagtaagg catcagcaat
     1981 cgcgtggagc agtacatcgg catctgagtg accatccaga ccacgaccat a
//
LOCUS       DASYWM010000063         5759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_283444, whole genome shotgun sequence.
ACCESSION   DASYWM010000063 DASYWM010000000
VERSION     DASYWM010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283444"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..126
                     /locus_tag="VGR29_02330"
     tRNA            53..126
                     /locus_tag="VGR29_02330"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87..89,aa:Glu,seq:ttc)
     gene            461..1438
                     /locus_tag="VGR29_02335"
     CDS             461..1438
                     /locus_tag="VGR29_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEV2072456.1"
                     /translation="MDIILPVAGFGTRLRPHTWSKPKPLVSVAGKAMMAHVLDRVMPV
                     KPGKLVFITGYLGEQIEAWARAQYPDVELAFVEQPEMLGQSDAIYRTRDFVDDDALML
                     FPDALFEADFSGLAGRDADGVAFTKHVDDPSAYGVALVEDGRVTQLVEKPSEPISNEA
                     VIGIYYFRKMEELYSAIEEQMGNDIKTKGEYFIADAIQIMIDRGARFEIERIDVWEDC
                     GNVEALLDTNRYLLSNEPPSPAQRPGSLIVPPSYVHRDARLEDAVIGPFASIGAGTVV
                     RNSVVRDSIVEENSEIVDAILQRSVVGSKTTIHGSARSISVGDNGKVAL"
     gene            1435..2409
                     /gene="prmA"
                     /locus_tag="VGR29_02340"
     CDS             1435..2409
                     /gene="prmA"
                     /locus_tag="VGR29_02340"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV2072457.1"
                     /translation="MTEPSAPHSESINAATNTPERTWYELSVDVDAEAVEAVSELFGR
                     YGFNEGVAIEEPYIQDSDGDNLGIALNKPFTIHTYVPEEDFRPEILEDLRHALYFLGQ
                     LRGVSDVRVSSLKEEDWANAWKDHFQVHKIGGRVVIRPPWRDYEPRDDEVVIVLDPGM
                     AFGTGLHPSTKLSMLGTEQVVKPGDTVLDVGTGSGILAIAAIKLGAAKADTVDVESVA
                     VAAAAQNAERNGVSDQVAVQQGSVGEGEPFYGQQYEVVLANIIARVLIELSEAIVGHT
                     RVDGSIVLAGIIESREQEVIDAFSHIGAEVVTRRFQDDWVSLVLCRTR"
     gene            2523..3959
                     /locus_tag="VGR29_02345"
     CDS             2523..3959
                     /locus_tag="VGR29_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEV2072458.1"
                     /translation="MPVVSPLSHRRVDLVPSVPGTVLAVSPGSLAMELGIEPGDRVLG
                     VNGKPLVDALDFQFQAQSERVVIDVDREGSILRYDLELEGDEFWGVTFEDPTFDGVRV
                     CENACPFCFIKQIPKGMRRSLYVMDDDFRYSMLYGSFVTLTNLSEEDWQRIEEQHISP
                     MHVSVHATNPDLRVELVGNPKAGKIMDDLARLERAGIDFHCQLVLVPGVNDGEEMDRS
                     LRDLSTFGERLKSIAGVPVGLSKHGQERQSKQVRLSRTCMRRLPGVNQEAIRVRRYKP
                     DEAAKVIDQAEVWQERFRNERGDTFYHLGDEFYLMTGREVPDADHYAGFPQVEDGIGI
                     TRFFLEEMEQYLRRARPGVLSGTAGTIACGTLVAPTMAEAMERFNAYTGSRLTVRGID
                     NNYLGTEINVSGLLSGADLIEQLKDEPANGPIFVTKKMISDRTGTLLDDTRIEDVASQ
                     LGRAIHPAEKMGEVAAYLRRLRPVLQVA"
     gene            complement(4049..4918)
                     /locus_tag="VGR29_02350"
     CDS             complement(4049..4918)
                     /locus_tag="VGR29_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2072459.1"
                     /translation="MQPSSNVTTAHDPSLAPIRIPGSLSLVLPAHNEAENIEIVIQRA
                     LAILPLHTEVFEVIPVNDGSKDATGQIIDRLSLMDERVRPVHHKVNRGYGGALKSGFA
                     ASRNDYVMFMDSDRQFDIADIQRLAPFVSSHDIVAGFRMERSDQMVRRINAEIFNLAV
                     RILFGVHLRDLDCAFKIFRGEQLRSLRLTTSGALINAEIQAKLRRQGAVLQQVGVPHY
                     PRIAGSATGGSIRVILRAMKEIIQLWVRMHKYQPPASAKSTRPYYALGDSLLTVALGA
                     VGSMAIRILRRRP"
     gene            complement(5012..5497)
                     /locus_tag="VGR29_02355"
     CDS             complement(5012..5497)
                     /locus_tag="VGR29_02355"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2072460.1"
                     /translation="MPELQLPDSFQTFDWFILALRVAFIFLVYFFLYQVARVTIRELV
                     TIGTATAGEGRGEHSVPDPSSALELLEPAESSYVRGSTFPLDHYTTVGRHSGNTLEID
                     DSFVSGSHAELMYEQGAWWLQDLNSTNGTFLNNQPVRNRSRLTEGDIVQFGRVRFRVL
                     I"
     gene            complement(5504..>5759)
                     /locus_tag="VGR29_02360"
     CDS             complement(5504..>5759)
                     /locus_tag="VGR29_02360"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2072461.1"
                     /translation="STIGRSPENDVVLDAPDVSRRHARIERNGKHLRIYDLNSTNGTR
                     VNGEAVHISDLEPGDEIRLGGQTLTVTSDGSSSRGQQSWR"
BASE COUNT         1193 a         1585 c         1777 g         1204 t
ORIGIN      
        1 gtaggaagag ttgacaaccg gtggcaaaag cgcgtatctt caaccgcgct gtgcccccat
       61 agtctagcgg cccaggacgt caccctttca aggtgaagat cgcgggttcg aatcccgctg
      121 ggggtaccga gagagccggg gcagagatgc cccggctttt ttgtgaccgc catatcgaga
      181 attcccggtg ggcgttattt cctgcatgcc gaagacgttt cgacgtccgc ttgggccttg
      241 gcccggcgcc aattgcgatg cggacgggct tacttccatg agcggctttg aacttgcgac
      301 gttggtcgcg ttgagccaac ttgccaaaag tccgcgagga ggcatctgtt catcgcggat
      361 caggtttttc gaccttcgca gttcttgatc tattgcgcgc ggcgggcgcg acatcgtctc
      421 aaacctaggc acgggcggcc cttaatatgg agaaggattc atggacatca ttcttccggt
      481 tgcaggattt ggaactcgtc tcaggccgca cacatggagc aagcccaagc cgctggtcag
      541 cgttgccggc aaggccatga tggcgcatgt gcttgaccgg gtgatgccgg tgaagccggg
      601 aaaactggtg tttattaccg gctatctggg cgagcagatc gaggcatggg cgcgtgctca
      661 gtatcctgat gtcgagcttg ccttcgtcga gcagccggag atgctcgggc agtcggacgc
      721 gatctatcgc acacgcgact ttgttgatga tgacgctctc atgcttttcc ccgacgcgct
      781 gttcgaggcg gatttctccg gcctagctgg tcgggacgcc gacggagttg cgttcacgaa
      841 acacgtagac gacccgtccg cctatggtgt ggccctcgtt gaggatgggc gagtgacgca
      901 gcttgttgag aagccgagcg agccgatttc gaacgaggcg gtgatcggga tttactactt
      961 cagaaagatg gaggagctct actctgcgat tgaagagcag atgggcaacg acatcaagac
     1021 gaagggcgag tacttcatcg ctgatgccat ccaaatcatg atcgaccggg gcgcgagatt
     1081 cgaaatcgag cggatcgatg tttgggagga ctgtggcaac gtcgaggccc tgcttgatac
     1141 caatcgctat ctcttgtcaa acgagccacc atcacccgcc cagcgcccgg ggtctctgat
     1201 cgttcccccg tcgtatgtgc accgcgatgc gcggctcgag gacgcggtaa ttgggccctt
     1261 cgcgagcatc ggcgcaggca ccgtcgtacg caactcggtg gtgcgggatt cgatcgttga
     1321 ggaaaactcg gagattgtag acgcaatcct ccagaggtct gtcgttgggt cgaaaacgac
     1381 gattcacggc agtgcacgtt cgattagcgt cggggataac gggaaggtag ctctgtgacc
     1441 gaaccgtcgg ctccgcatag cgagtccatc aacgccgcta cgaacacccc agaacgcacg
     1501 tggtacgaac tatcggtgga tgtggacgcg gaagccgtgg aggcggtgtc ggagcttttc
     1561 ggccgctatg gcttcaacga gggtgttgcc atcgaggaac cctatatcca agatagtgac
     1621 ggggacaacc tcggaatcgc gctcaacaag ccctttacaa ttcacactta tgtccctgag
     1681 gaagacttcc gcccggagat cctcgaggat ctccggcacg ctctttactt tctcgggcag
     1741 ctgcgcggcg tgagcgatgt tcgcgtttcg tcgctcaaag aggaggactg ggcgaacgcc
     1801 tggaaggacc atttccaggt ccacaagatc ggcggccggg tggtgatacg cccgccctgg
     1861 cgcgactacg agccaaggga cgatgaagtg gtcatcgtgc tggatccggg catggcgttc
     1921 ggcacgggct tgcacccgtc aacgaagctc tcgatgcttg gtaccgaaca agtggtaaaa
     1981 cccggtgaca ccgtgctgga cgttggtacc ggttcaggaa tcctcgcaat cgccgcgatc
     2041 aagcttggtg cagcgaaagc ggatactgtc gatgtcgaga gtgtcgctgt cgcggcggcc
     2101 gcccagaacg cagagcgaaa tggcgtatca gatcaggtcg cagtgcagca gggcagcgtc
     2161 ggagagggag agccgttcta cgggcagcaa tacgaggttg tgctggccaa tatcatcgct
     2221 cgggtcctga tcgagctctc tgaagcgatc gtgggacaca ccagggtgga tggctccatt
     2281 gttctcgctg gcatcataga aagccgggag caggaggtaa tcgacgcgtt ctcccatatt
     2341 ggggcagagg tcgtgacacg acgctttcag gatgactggg tctccctcgt gctgtgtcgc
     2401 accagatgag ggtattgagc cggatggtat aacttgtagt ttggcgcgcc aacccgtgtc
     2461 cattttccac gtggcgcact catattcttg caccttcgat caatagaaag ccgttccctc
     2521 caatgcccgt cgtcagccca ctctctcatc gtcgcgtaga tttggtcccc agcgtaccgg
     2581 ggaccgtcct cgctgtctct cccggttccc tggcgatgga gctcgggatt gagccgggtg
     2641 atcgtgtgct cggcgtgaac ggtaagcccc ttgttgatgc cctggatttc cagttccagg
     2701 cgcagtccga gcgcgtcgta attgatgtgg accgggaggg atcgatcctc cggtacgatc
     2761 tcgagttgga aggggatgag ttctggggcg tgaccttcga ggacccgacc tttgacggcg
     2821 tccgcgtttg cgagaatgcg tgtcctttct gcttcattaa gcagattcca aaaggcatga
     2881 ggcgctcgct ttatgttatg gacgatgatt ttcggtattc gatgctctat ggctccttcg
     2941 tgaccctgac gaacctatcg gaggaggact ggcagcggat cgaggaacag cacatcagtc
     3001 cgatgcatgt atcggtacac gcgacgaatc ctgatctcag ggttgagttg gtcggaaacc
     3061 caaaggcagg gaagatcatg gacgatcttg cgcgcctcga gcgtgcggga atcgattttc
     3121 actgtcagct cgtgttggtc cccggcgtta acgacggcga ggagatggac cgtagcctgc
     3181 gcgatctctc aacattcggt gagcgcttga agtcgattgc cggagtgccg gtcgggttga
     3241 gcaagcatgg tcaggagcgg cagagcaagc aggtgaggct ctctcgcacg tgtatgcggc
     3301 ggttgccagg agtcaaccag gaagcaatca gggtccgccg ctacaagcca gacgaagctg
     3361 ctaaggtaat cgatcaggct gaagtttggc aggaacgctt ccgcaatgaa cggggcgaca
     3421 ccttctatca cctcggtgat gagttctacc tcatgaccgg ccgcgaggtt cctgatgccg
     3481 accactatgc gggtttccct caagtcgaag atgggatcgg gatcacccgg ttctttctcg
     3541 aggagatgga gcagtatctc cggcgggcgc gtccgggtgt tctcagtgga acggcgggga
     3601 ctattgcttg tgggacgctg gtcgcgccca caatggcaga ggcgatggag cgattcaacg
     3661 cctataccgg ctctcgtctc acagtgcgag ggatcgacaa caactatctc ggtaccgaga
     3721 tcaacgtgtc agggctgctg agcggcgctg acctgatcga gcagctgaag gacgagccgg
     3781 caaacggtcc gatcttcgtc acaaaaaaga tgatctccga cagaacggga acattacttg
     3841 acgacacccg catagaggat gtcgcctccc agctcggtcg cgcgattcac ccggctgaga
     3901 agatggggga ggttgccgcc tacctgcgcc ggctacgacc cgttcttcaa gtcgcctgat
     3961 ctcatgactt aagtcgcgca cgacaacgaa aaggggatgg cgttggtgcc atcccctttt
     4021 cgttgtcgcg atcctcgtat gagcacgcct agggccggcg gcgcagtatc cgaatggcca
     4081 tacttcccac cgcaccgaga gcaaccgtca gcagcgagtc gccaagtgcg tagtagggcc
     4141 gggtgctctt ggccgatgct ggcggctggt acttgtgcat acggacccac agctggatga
     4201 tttctttcat cgcgcggagg atgacacgta tgctgccacc cgtggcggag ccagcaatgc
     4261 gggggtaatg aggcactcca acttgctgca ggacagctcc ctgccggcgt agcttcgcct
     4321 ggatttccgc attgatgagc gcgccggatg tggtaagtcg cagcgatcgc agttgctctc
     4381 ctcggaaaat cttgaacgcg caatccaggt cccgaagatg cactccgaac aatatgcgga
     4441 ccgcaagatt gaaaatctcc gcgttgatgc gccgaaccat ttgatcggag cgttccatac
     4501 ggaaccccgc cacgatgtcg tgcgaggaga cgaacggcgc cagccgttga atatccgcga
     4561 tatcgaactg gcgatccgaa tccataaaca tcacgtaatc gttacgagat gctgcgaatc
     4621 ccgacttcag cgccccgcca tagccccggt tcaccttgtg gtgaacaggt cgcactcgtt
     4681 cgtccatcaa ggacagccgg tcaatgattt ggccagtggc gtccttgctg ccatcgttga
     4741 ccggaatgac ctcaaagacc tcagtgtgga ggggaaggat cgcgagtgcc cgctggatga
     4801 cgatctcgat gttctcagcc tcattgtggg ctggtagcac gagcgaaagt gaacccggaa
     4861 tgcggatggg agcgagcgag gggtcatgtg ccgttgtcac attggaggac ggttgcacga
     4921 gtaggttgcc tccccgaaga gcagaagcaa ggacgcaccg tatagcctcc atgcggtgga
     4981 gaaagagcat cggtagtgtc tcagactttc gttagatgag gacccggaag cggacccggc
     5041 caaattgtac gatgtccccc tctgtcagcc gagaccgatt acggaccggc tggttattca
     5101 ggaaggttcc gttcgtgctg ttcaaatcct gtagccacca ggcgccttgt tcgtacatca
     5161 gttcggcatg agaccccgac acgaagctat cgtcaatctc aagtgtattc ccgctgtgcc
     5221 gacccacagt ggtgtaatgg tccaagggaa aggtgctgcc acgtacatac gacgactcgg
     5281 cgggctcaag aagttccagt gcgctggatg ggtccggcac tgagtgctcc ccacgcccct
     5341 cgccggccgt cgcggtcccg atcgtcacca actcgcgtat ggtgacacgg gccacttgat
     5401 agagaaagaa atacaccaga aagatgaatg caacgcgcag ggcgaggatg aaccagtcga
     5461 acgtttgaaa cgagtcaggc agctgcagct ccggcatgct ccgctaccgc caactctgct
     5521 ggccgcggct gctagatccg tcggaggtca cggtaagcgt ctgaccgcca agtcgaatct
     5581 catctcccgg ctcaagatcg gaaatatgga cggcctcgcc atttacacga gttccgttgg
     5641 tgctgttgag atcgtaaatc ctgagatgtt tcccgttgcg ttctatgcgc gcatggcgtc
     5701 gtgatacgtc cggggcatcc agaacgacgt cattctcagg cgaacgaccg atcgttgag
//
LOCUS       DASYWM010000064        14338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_189718, whole genome shotgun sequence.
ACCESSION   DASYWM010000064 DASYWM010000000
VERSION     DASYWM010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14338
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189718"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VGR29_02365"
     CDS             complement(<1..1160)
                     /locus_tag="VGR29_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2072462.1"
                     /translation="MPTIDAEYRAKFGDSATMFDRAQKVIAGGITHDGRFIKPYPPYF
                     ASANGAYKWSVEGHKVIDYGIGHGSLIFGHNDPDLTAAMVNQAPNGTHHSAGHEAEIR
                     WAEKITELVPSAELVRFTASGTESTLLAMRVARGYSDKRIILKFEGHFHGWQDYALKG
                     EKPPFERTTVPGIPHETLSTVAVVPSNDLAMLEERLVQGDVAGVIVEPSGASWASIPL
                     QEDFLKNVRALTEKYGVVLIFDEVITGFRWAPGGAQQRFGVTPDVTTMAKIVAGGMPG
                     GAVAGKREIMEMIAFSDDPTFNKTRRVPQAGTYNANPLAAAAGTAMLTKCANPSVQQK
                     CDDMAARLRAGINQVIARLDVPAFAWGESSVFHIAAGQTCSNFTAGDMHTPEG"
     gene            1443..2699
                     /gene="hemA"
                     /locus_tag="VGR29_02370"
     CDS             1443..2699
                     /gene="hemA"
                     /locus_tag="VGR29_02370"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEV2072463.1"
                     /translation="MQVSTPTIVVVGSNHEYAPVDIREQLAFSGDQLARGLNALQQRV
                     PEGLILSTCNRTEIYAVGQSDEQVVAEIFGFLSGYHSVTEPMLRRSSYVLSGEQAVDH
                     FFRVASGLNSMILGEPQILSQIREALDLAREQNAVGPMLQRLTLDALRIGKRARTETD
                     IARNRVSIAHAAVDLAERELNGLSGLSVAILGAGKMATLAAKLLRARGASEISVVNRS
                     VDRARELAESVDGVALPLTALEDAIASAHLVIGAAVSDGPLISAAHVAPRNTPVLMID
                     LSVPRVVDQAVGHDPLVQVRDVDALDPIAEENRRRYSSEVRKVETLVLEATRNYERWV
                     ESRRGVQAIAAVRARAEAIRDLELDKALRRLQHLSDRDQNIVRVLACGLTNKLLHQPV
                     QELRAVGDVDQRQAILRAFGVEEPDT"
     gene            2748..4061
                     /gene="hemL"
                     /locus_tag="VGR29_02375"
     CDS             2748..4061
                     /gene="hemL"
                     /locus_tag="VGR29_02375"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEV2072464.1"
                     /translation="MSQSPVSSRSVQRSATAFDAAKQVIPGGVNSPVRAFRAVGGAPL
                     FIDRGEGSRFQDLDGNWYIDYVLSWGPLILGHAHPEVIEATTKALEKGTSFGAPTLAE
                     TELAGMIVSSIPAVDEVRLVNSGTEATMSALRLARAATGRDKIVKFAGCHHGHSDMLL
                     VQAGSGVATLGLPDSPGVPKGATVDTLVAPYNDLVAVEQLFAEAGEKIAAVIVEPVAG
                     NMGMIPPVDGFLEGLRRITQTHGAILIFDEVMTGFRVSPTGAVGLYGIEPDLVTFGKV
                     VGGGFPLAAYAGKRDIMEHVAPAGTMYQAGTLSGNPVATAAGIATLKVLQRKGVWQGI
                     AAQAGKLVDGLRQAAAGTGVPVQAANVGTMAGMFFIDEPVHSYDDAKGSDVEAFGRFF
                     MEMLDRGVYFAPSQFEALFLSSAHTDEDIDRTVCAAAEAFQTPRR"
     gene            complement(4243..5088)
                     /locus_tag="VGR29_02380"
     CDS             complement(4243..5088)
                     /locus_tag="VGR29_02380"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV2072465.1"
                     /translation="MNGSSGSTVFDTSEHVSIMSFNLKNGGNIISWIRRRALLVDVIR
                     EANPILIGTQEGYRSQLAYIRNKLPGYEFVGASRYPDEVDEYNAIFFDTSQVNVADSG
                     TIWLSDTPDVPGSMFETEHMPRIATWALCDIIGYDRQLLSVNTHLTYEDAGIDAQVDV
                     LIEQLDKFAPEDVDIVLTGDFNQPRHSRAWDKLVGIGFVDAWEFATSESGPRITAHNW
                     KGAPSGDDAGSTPERRIDWIMYRPANGTTLPRNCIMETVDTHNGTVYPSDHFPIVLRN
                     QPHHS"
     gene            complement(5147..5785)
                     /locus_tag="VGR29_02385"
     CDS             complement(5147..5785)
                     /locus_tag="VGR29_02385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2072466.1"
                     /translation="MSISETFKYEPSVKVDTSNYRKHTSDNPIQRRLIDRFHAKITEI
                     VTDLRPRTLLDAGCGEGFVDEIFLAAMPELQITGFDVLEDSVKLASMRNPRATFSSGD
                     IYNIDHPDNSFDVVVAFEVMEHLHEPDKGLSEMARVAKEYVVLSVPHEPFFCLANAAR
                     GKNLDINPKGSDPDHRNFWSREKFGEFVSRELDVVTLTGSFPWTICVGRPRR"
     gene            complement(5814..6872)
                     /locus_tag="VGR29_02390"
     CDS             complement(5814..6872)
                     /locus_tag="VGR29_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV2072467.1"
                     /translation="MSLADGNPERDAPTRPKARGAGQGFLGAIARTPTPLIFISCVIL
                     AILLLWWQGQVHEIISIVARARLQPLLIAAPIYVVSLWLLCLRWHLLVRMAQGWSDFP
                     RASEAFLTSVVINYAAPIGLAVPSRAALTKRALGLDHGTTGTIALWEIGADVLVLGAG
                     SVLWLFLAEGSTSAVGSELSASAGQYARWGGVAIVLVLLTLAWSLRSPGRRRRFLGLA
                     QKILVAPSVRPIEALMSIGVTLVYWMIQGVVLALLVHAMRVDISFEFILGLTSVPILV
                     GMLSPIPGGAVVREALMYVVARLADVPAGEVVAAAVIYRIALFGAIPILYALTRWWIS
                     RRVHHQSNPSRHEPVGTA"
     gene            complement(6869..7906)
                     /locus_tag="VGR29_02395"
     CDS             complement(6869..7906)
                     /locus_tag="VGR29_02395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2072468.1"
                     /translation="MANAPRIVSDISEEPIRSLTILIPVMNEEGNLLALYERLTASLE
                     SIALPYEIIFVDDGSRDNTWKLVAELSAQDHRVIGLKHRRNFGKAQALSNGFAAARGD
                     VIVTMDGDLQDDPDELPRFLDMLNDGCDLVSGWKQRRQDPLGKTFPSKLFNWTVRKAS
                     GVPLHDFNCGFKAYRREVTSSIRLYGELHRFTPVLASAEGFRIGELPVKHHARQWGSS
                     KYGWSRLTKGFLDLLTVVFLTQFRQRPMHLFGLPGMIFMTIGFLIGLWLSFEKLILQH
                     PIGTRPLLLLAVLLIVIGTQFFGLGLLGEYQSYGSNAPHPDRPIPLSGTVGLASRPEL
                     TQSAVKPGIAR"
     gene            complement(8105..9013)
                     /locus_tag="VGR29_02400"
     CDS             complement(8105..9013)
                     /locus_tag="VGR29_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072469.1"
                     /translation="MTHQHPGPDASHGAMNTDCTFFQENAEAFALDVVEGTERSRMEQ
                     HMLWCGPCRRGLAEIRRVTDLLPFLSPPALPSPSAKARLFERIAEETTDDAESIATFS
                     NPWIPTTPVDRPERAARKGVQTGAWQRWIAPGLIAPLVICLIILSAWTNSLRNEVSML
                     RSAEASRASAAVGAPPQSDMQLYAFRPACEKCKEKQAAGQFGGNPEGSIGVVVAWDLD
                     PNEKHQVWCVDSQGQKRLVSDLEVEQTGSVFQAVSFPEPIGGYEQIYVAQHDGTADPA
                     AELMVAMNEEHEVDALDATPGDPVTD"
     gene            complement(9010..9615)
                     /locus_tag="VGR29_02405"
     CDS             complement(9010..9615)
                     /locus_tag="VGR29_02405"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2072470.1"
                     /translation="MRQHHRVSENLAELADAELVDAMAAGDTRAMDVLYDRYSRAVFS
                     FALRLLGDRAVAEELLQEVFFRSWRQARKFSEGKGTFITWLLSITHNMAIDEIRKQNR
                     RPQRADSADPVLLLTNMTDGAVPVEDQAVFRTVRDQIATAMTLLPETQRSAIQLAYFS
                     GLTQREIAELQGEPLGTIKTRMRLAIRKLREHLVDQGVELQ"
     gene            complement(9780..11150)
                     /locus_tag="VGR29_02410"
     CDS             complement(9780..11150)
                     /locus_tag="VGR29_02410"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEV2072471.1"
                     /translation="MVKQVDESEQHRYRAALATIWDRSSYDRGFINNPFAGDDEARRG
                     LARTSAVLDLLGHPQERIQLVHVAGSKGKGSTSVLLDAILRAAGMRSGRFTSPHLHSY
                     RERFIVDDELITEGAFASLTEQVMEAVRKAESTTPDLGRITAWELSTAMALTWFAERM
                     CDVAVIEVGLGGTLDATNVIDPVVSAITRLDFEHTAILGETLPEIASNKAGIIKPGRP
                     SVTVEQPDAALSVIQERARHVGSPLLVQNRDWFVEGDEDKFAVTGPWGSDEKLQTSLV
                     GPHQVENAALAVAAIKMAFSDDSAVDYLAIRAGLRSAHHPGRFERVVREDRTIIIDGA
                     HTPIAADALSAALTRHVSTEPVTFVIGMLQDKRPDPFLKPLIPRADRWIVARLDSPRT
                     MPVDLLSEALSTLGQRVNGAPSIAAALDLALKDGRNTVVVTGSLTAAAEARVHLGLAS
                     PDPSPT"
     gene            11280..13379
                     /locus_tag="VGR29_02415"
     CDS             11280..13379
                     /locus_tag="VGR29_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV2072472.1"
                     /translation="MEVTKHDMEATPSIATRREDVVEVLHGHQIHDPYRWLEDGSSDE
                     TRAWTRDQNARTEAVLAGIPGREQLEGQLRELLQVGSVTGARETGGRFFYTRREGSQD
                     QAVLYVREGLDGEERTLLDPSAQGEHGLVALDWWEPSHDGTRLAYGVSQGGDEWSVLH
                     VFDVGTGEDLDEAIPRARAASVAWLPDNNGFYYTRYPLPGSVPDGELHYHRRVFFHLI
                     GSDYHDDPLVFGGDRPKELLPAVSIEPTGRWVIVEENEGWIRTGIYVLDRQHEEAGFR
                     DITPDGAAIHEVLGIDNGVLYDLTQWQAPNGQIAAYSLENGNEKGQAAWSTVVAGRED
                     RVIEYAAITPGGIVTGELEAAMGKVRRYGRDGSSRGDLGLPGIGSITGLHASPSSDIV
                     VVGYQSFVQPPTALVYQGDDQAPVELSPVAPPPGFDPDTCEIRQVWYASKDGQNISMF
                     LVHRKGLELDGSNPVYLTGYGGFNVTRGSEYQPALPFWLGHGGVFALPNLRGGAEYGA
                     AWHEAGMLAVKQNTFNDFIAAAEYLIAEGYTRPERLGIAGGSNGGLLVGAVVTQRPEL
                     FRAAICMVPLLDMLRYHLFRIARLWIPEYGSSEDAEQFAWLHAYSPYHAVREGVEYPA
                     MLLTLGENDSRVDPMHARKMTALLQRATGSGADRPILLRAESDAGHGQGKPLWMRVQE
                     AADLWGFMSWQLGIDLA"
     gene            complement(13835..>14338)
                     /locus_tag="VGR29_02420"
     CDS             complement(13835..>14338)
                     /locus_tag="VGR29_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HEV2072473.1"
                     /translation="VTSVIDIQPTTGDKGAVSDIAQDLLKTVCVERHHATGQIGVGFT
                     RGFGLARGALATTVSHDSHNILAVGVSDDDLLAAIALVAESQGGMAVVADGQVLGHLP
                     LPIAGLMSDRPLFEVAATYEALGSAARSLGCKLDDPFGQLAFLALSVIPEGRITHTGL
                     LDLTVAH"
BASE COUNT         2795 a         4134 c         4545 g         2864 t
ORIGIN      
        1 ccctcgggag tgtgcatgtc ccccgccgtg aagttggagc atgtttggcc ggcggcgata
       61 tggaagacgg agctctctcc ccatgcgaag gcagggacat cgagcctggc gatcacttga
      121 ttgatccccg cccggagcct tgccgccatg tcgtcgcatt tctgctgaac tgacgggttt
      181 gcgcacttag tgagcatggc cgttccagcg gcggctgcca gcgggttggc gttgtacgta
      241 ccggcctgtg gcacgcgacg agtcttgttg aaagtgggat cgtcgctgaa ggcaatcatc
      301 tccatgatct cgcgcttgcc ggcaacggct ccgccgggca tgccgccagc aacgatcttg
      361 gccatcgttg tgacgtccgg ggtgaccccg aagcgttgct gggcgccacc tggagcccat
      421 cggaagccgg tgatgacctc gtcgaagatc agcaccaccc cgtatttttc ggtcagtgcg
      481 cggacgttct tcaggaaatc ctcctgaagc ggaatcgacg cccaggacgc gccgctcggc
      541 tccacgatca ctccggcgac atcgccttgt acgagacgct cctccagcat ggcgaggtcg
      601 tttgatggca ctaccgccac cgtgctcagc gtctcgtgcg ggatgcctgg caccgtggtc
      661 cgctcaaatg ggggcttttc gcccttcagc gcgtaatcct gccagccatg gaaatggccc
      721 tcgaacttga gaatgatcct cttgtcgcta tatccccggg ccacacgcat ggcaagcagg
      781 gtggactccg tacctgaggc ggtgaatcgc accagctcgg ctgacggcac aagttcggtg
      841 atcttctctg cccaacggat ctctgcctca tgtccggcgc tgtggtgggt tccgtttggt
      901 gcctggttaa ccatcgcagc ggtgagatca ggatcattgt gaccgaagat cagtgatcca
      961 tggccgatgc catagtcgat caccttgtga ccttcgaccg accacttgta cgccccgttc
     1021 gcgctggcga agtagggtgg gtagggcttg atgaagcgtc catcatgagt gatgccgccc
     1081 gcaatgacct tctgggcgcg gtcgaacatc gtggccgaat caccaaattt cgcgcgatac
     1141 tcggcgtcga tcgtgggcat caatattctc ctcatgcgca tggtgcgtgt cggtttcatc
     1201 atctttcggc actgtatcca aggataacga tttcgcctac ccccggttgg aggagtgcgc
     1261 ccttgctgac caaccgtaat gagacttatt cctgaagcac tgccagaaag gacgcctacc
     1321 ccctgttcct cgggtatcat tagtggtgac agagtccgga tggtcccggc cgtcagacga
     1381 acggggcgca agctccggac gacgggaacg acccgtctgc aacctccata gagtacgccc
     1441 acatgcaggt ttcgactcca acgatcgtgg tcgttggttc taatcatgaa tatgccccgg
     1501 ttgatatccg tgaacaactg gcgttctcgg gtgatcaact tgcccgaggg ctaaacgccc
     1561 tgcaacagcg ggttcccgaa gggctcatcc tttcgacctg caaccgtacg gagatttacg
     1621 ctgtcgggca gtccgatgag caggttgtgg ccgagatctt tggcttcctg agcggctatc
     1681 acagtgtcac ggagccaatg ctccgcaggt cttcgtatgt gctctcgggc gagcaggctg
     1741 tcgatcattt ttttcgggta gccagcggtc tcaactccat gattcttggc gagccgcaga
     1801 ttctctccca gattcgcgag gcgcttgatc ttgcacggga gcagaacgcg gtgggcccga
     1861 tgctgcagcg gctgacgctt gatgcgctac ggattggaaa gagggcacgc accgagacgg
     1921 acatcgcgcg taaccgtgtc tccattgcgc atgccgccgt tgacctcgca gagagggagc
     1981 tgaatgggct gtccggcctg tcggtagcaa tccttggggc aggcaagatg gcgacgcttg
     2041 ccgcgaagct cctgcgggcg cgtggagcct ccgagatcag cgtggtcaac aggtctgtag
     2101 accgggccag agaactcgcg gagagtgtcg atggggtcgc actgccgctc acggcactcg
     2161 aagatgccat cgcatccgct catctcgtca tcggtgcggc ggtctcggac ggaccgctca
     2221 tctcggcagc acatgtggcc ccccgtaata ccccggtgct gatgatcgat ctcagcgtgc
     2281 cgcgagtcgt tgaccaggca gtcgggcacg atccattggt gcaggtgcgt gatgtggatg
     2341 cactggatcc gattgccgag gagaaccggc gcaggtattc ctccgaggtg cgtaaggtcg
     2401 agacgctcgt gcttgaggct acccgcaatt atgagcggtg ggttgagagc aggcgggggg
     2461 ttcaggcgat tgcggcggtt cgcgccaggg cggaagcgat tcgggacctg gagctagata
     2521 aagccctccg acggctacag cacctttctg atcgtgacca aaacatcgtc cgcgtgctgg
     2581 cgtgcggcct caccaacaag ttgcttcatc agcctgtcca ggaactcagg gctgtaggcg
     2641 atgtagacca gcgccaggcg atcctgcggg ccttcggcgt ggaagaaccc gacacctgag
     2701 gcacctcggc ctcaactccg catctcgatc cagacaggag cctatctgtg tcccagtccc
     2761 ccgtgtcgtc acgttctgtc cagcgttctg ctaccgcttt cgacgcggca aaacaggtga
     2821 tcccgggggg agtcaatagc ccggttcgtg cttttcgtgc agtcggtggc gcgccgctct
     2881 ttatcgatcg tggcgagggt agccggtttc aggatctgga cggaaactgg tacatcgatt
     2941 acgtgctttc ctgggggcca ctcatcctcg ggcatgcgca tcccgaggtg atcgaggcga
     3001 cgacgaaggc cctggagaag ggcacaagct tcggggcacc gacgctggcc gagaccgagc
     3061 ttgccgggat gatcgtttcc agcatcccgg ccgtagacga ggtgaggttg gtcaactcgg
     3121 gtaccgaagc gacgatgagt gccctccgcc tcgcacgagc ggccacagga cgggacaaga
     3181 ttgttaagtt cgccggttgc catcatggtc actcagatat gctgctggta caggcaggaa
     3241 gcggagtggc gacgctcggt ttgccggata gtcccggggt gccgaagggg gcaaccgtcg
     3301 atacgctggt tgctccatac aatgacctcg tggctgttga gcagctcttc gccgaggcgg
     3361 gcgagaagat cgccgcggtg atcgttgagc cggtagccgg caacatggga atgatcccgc
     3421 cagtggatgg cttcctcgag gggttgcggc gtatcactca aacccatggc gcgatcctga
     3481 ttttcgacga ggtaatgaca ggattccggg tctcacctac gggagctgtt ggcctgtatg
     3541 ggatcgagcc ggacctggtg acgttcggca aggttgtggg cggcgggttc ccgctcgctg
     3601 cctatgcggg aaagcgcgac atcatggagc acgtggcgcc ggccggaacg atgtaccagg
     3661 caggaacgct ttccgggaat cctgtggcga cggcggccgg tatcgcgacg ctaaaggtcc
     3721 tgcagcgcaa aggcgtctgg cagggcatcg cggcccaggc cgggaagctg gtcgatggat
     3781 tgcggcaggc cgcggccggg accggcgtcc cggtgcaggc cgccaacgtc ggcacgatgg
     3841 ctggtatgtt cttcatcgac gagccggtgc attcgtacga tgatgccaag gggagcgatg
     3901 tcgaggcatt cggccgcttc ttcatggaga tgctcgatcg cggggtctac ttcgcgccgt
     3961 cgcagttcga ggcacttttt ctctcgtccg ctcacaccga tgaggatatc gatcgtacgg
     4021 tctgcgctgc cgcagaggcg ttccagactc ccaggagata gcgccaagcc ctctgttagc
     4081 gtctatcgga ggtagtgcct cagggctgtc accggcccat acccttgcgt tgtcgtcgaa
     4141 gccttaccat ctcttctggt gtcagtgaca gcacttgctg tggccaggca cccttccaca
     4201 tcgcgtttag aggtgttcag tccatcggtt cgcgacgatc gatcacgagt ggtgtggttg
     4261 gttgcgaagc acaattggga agtggtctga ggggtagacg gtcccattgt gggtatcgac
     4321 ggtttccatg atgcagttgc gcggtagcgt cgttccattg gcgggccggt acatgatcca
     4381 gtcgatccgc cgttccgggg tgctaccggc gtcatcgccg gaaggcgctc ctttccagtt
     4441 atgtgctgtg atccgcggac cagattctga tgtcgcgaac tcccacgcat caacgaagcc
     4501 aatcccgaca agcttgtccc atgcacggga atgcctcggt tgattgaaat cgcctgttaa
     4561 gacgatatcc acatcctccg gggcgaactt gtccagttgc tctatgagca cgtcgacctg
     4621 cgcgtcgatg cccgcatcct cataggtgag atgggtgttc accgatagca gttggcgatc
     4681 ataaccgatg atgtcgcaca gcgcccacgt cgcgatgcgt ggcatatgtt cggtttcaaa
     4741 catgctgcct ggaacatccg gtgtgtcaga tagccagatc gttccgctgt ccgccacgtt
     4801 tacctgcgaa gtgtcgaaaa agatcgcgtt gtattcgtcg acttcgtccg gatatctgct
     4861 tgctccaacg aattcgtagc cgggaagttt gttgcggata tacgcaagtt gggaacgata
     4921 cccctcctgt gtcccgatca ggatcgggtt ggcctcccgg atgacgtcga ccagtaatgc
     4981 cctgcggcga atccatgaga tgatattccc gccgttcttg agattgaagg acatgatcga
     5041 gacgtgttcg gaggtgtcga acacggttga tcccgatgat ccgttcatca gcggctcctg
     5101 cctgccgaca atcacctagg gagacacgtc gccagggtgt atcgggctac cggcgtggtc
     5161 ggccgacgca gatagtccac ggaaaggagc cggtcagcgt cacgacgtcg agctcccgag
     5221 agacgaactc accgaacttc tcgcgagacc agaagttacg gtgatccgga tcgctgccct
     5281 ttgggttgat atcgagattc ttgccgcgtg cggcattggc gaggcagaag aatggctcgt
     5341 gaggtacgct gaggaccaca tactccttgg ctacgcgtgc catctcggag agacccttgt
     5401 ccggctcgtg gagatgctcc atgacctcga aggcgacaac gacatcgaag ctgttgtccg
     5461 ggtgatcgat attgtagata tcgccactgg agaatgtcgc ccgagggttt cgcatgctgg
     5521 cgagttttac cgaatcctca agcacatcga agcctgtgat ctgcagttcc ggcattgcgg
     5581 cgagaaatat ttcgtcgaca aagccctctc cacagcccgc atcgagcagg gtgcgtgggc
     5641 ggagatcggt cacgatctcg gtgatctttg catggaagcg gtcgatcagc cggcgttgga
     5701 tcgggttgtc gcttgtgtgc ttccggtagt tgctggtgtc tacctttacc gatggctcat
     5761 atttgaaggt ctccgagata ctcatgcgcc tggtagtcct ttcagtatca cgatcatgcg
     5821 gtcccgacgg gctcgtgccg ggaagggttc gattggtgat ggacacggcg ggagatccac
     5881 caccgggtca atgcatacag gatcgggatc gcaccaaaga gcgcgatgcg atagatgacg
     5941 gcggcagcca cgacctcccc ggccgggaca tcggcgagtc gagcaacaac gtacatgagc
     6001 gcctcccgta ctaccgcgcc gcccggaatg gggctcaaca tgccgacaag aatcgggacg
     6061 ctggtgaggc cgaggatgaa ttcgaaggag atatcgacac gcatggcatg caccaggagc
     6121 gccaacacga caccctggat catccagtag acgagcgtta ccccgatcga catcagggcc
     6181 tcgatgggcc gcacgctagg ggcgaccagg atcttctggg ccaagccgag gaatcgtcgg
     6241 cggcggccgg gacttcgcag cgaccaagcg agcgtaagga gaacaaggac gattgcgaca
     6301 ccaccccacc tggcgtattg tcccgcagag gctgagagct cgctcccaac cgctgaggtg
     6361 gagccttctg ccaggaagag ccagaggact gatccggcgc cgagcaccag cacatcggcc
     6421 ccgatctccc agagggcaat ggtgccagtc gtgccatggt cgagaccgag tgcgcgcttg
     6481 gtaagtgcgg cgcgggaggg gacggccagg cctatgggtg cggcgtagtt gatgaccacc
     6541 gacgtaagga acgcctcaga ggctctgggg aaatcggacc agccctgggc catgcggaca
     6601 agcaggtgcc accgcaggca gagcagccag aggctcacta catagatagg ggcggcgatg
     6661 aggaggggct ggagccgggc acgggccacg atggaaatga tctcatgtac ctggccttgc
     6721 caccagagca ggaggatggc caggatcacg cacgaaatga agatgagcgg ggtgggtgtg
     6781 cgggcgatcg cgccgaggaa gccttgtccc gcaccacgcg ccttcggccg cgtgggcgca
     6841 tctctttccg ggtttccgtc cgccagactc acctggcgat tccaggcttc accgctgatt
     6901 gggtgagttc tggtcgggat gccaggccca ctgtcccact aaggggaatc gggcgatcgg
     6961 gatgcggggc attggaacca taggactggt attcgcccag caggccaagc ccaaagaact
     7021 gggtgccgat gacgatcaga agcacggcga ggagaagcag aggtcgcgtg ccgatggggt
     7081 gctgcaggat gagtttctcg aatgagagcc acagaccaat caggaacccg atcgtcatga
     7141 agatcatgcc aggcagccca aagagatgca tggggcgctg gcggaactgc gtgaggaaga
     7201 cgacggtcag aagatcgagg aaacctttgg tcaggcgcga ccagccatac ttggacgagc
     7261 cccactgacg ggcatggtgc ttgacgggca attcgccgat gcggaatcct tcggcgctcg
     7321 caagcaccgg agtgaagcga tggagctcac cgtaaaggcg gatcgaggag gtcacctcac
     7381 ggcggtatgc cttgaaaccg caattgaagt cgtgtagcgg caccccggac gccttgcgga
     7441 ccgtccagtt gaagagcttg ctcgggaacg tcttgcctag tggatcctgg cggcgctgct
     7501 tccaaccgga aacgagatcg cagccatcgt tgagcatgtc caggaagcgc ggcagctcgt
     7561 ccgggtcatc ctgcaggtca ccatccatcg tcacgatcac atcgccgcga gcagcagcaa
     7621 agccgttgga aagtgcttgc gcctttccaa agttgcgccg gtgcttcagg ccgataaccc
     7681 gatgatcctg cgcgctgagc tcggctacca gtttccatgt gttgtcacgc gagccgtcgt
     7741 cgacaaagat aatttcgtag ggcagcgcga tggattcgag cgaagcggtc agtcgctcgt
     7801 agagcgcgag aagattgcct tcctcattca tcacggggat caggatggtc agcgaccgaa
     7861 tcggttcctc gctgatatca gaaacgatcc gtggggcatt tgccatggaa acgggaaaat
     7921 cctcgctggg ctcgtcaaaa gaagatgcag taagcgcaga acgcacagtg agttaactcc
     7981 cgcaactgtt cggacgtgcc acgtcggtcg cgtcacggct gccataactt atggactata
     8041 gaccgatggc gtgtgcagcg caaatccgaa gccgaaacga tattgcctgg ctgcggcaag
     8101 ggcgttagtc ggtcaccggg tcgccaggcg tggcatccag agcgtcgact tcatgctcct
     8161 cattcatggc gaccatcaat tctgccgccg ggtcggcggt gccgtcatgc tgtgccacgt
     8221 agatttgctc atagccaccg atgggctccg ggaaggagac ggcctggaag acacttcccg
     8281 tctgctctac ctcgagatcg gagacgagcc tcttttgacc ctggctgtcg acacaccaga
     8341 cctggtgctt ctcattggga tcgagatccc aggctaccac gacgccgatg cttccctcgg
     8401 ggttgccccc gaactggcct gcggcctgct tctccttgca cttctcgcat gcgggacgga
     8461 aagcatagag ctgcatgtcg gattggggag gtgccccaac ggcagcgctc gctcgcgaag
     8521 cctcggcgga gcgcagcatg ctgacctcat tccgcaatga gtttgtccat gccgagagta
     8581 tgatcaggca aatgaccagc ggagcgatga gcccaggagc aatccagcgc tgccaggcac
     8641 ctgtttgcac tcctttgcgg gcggctcgtt ccggtcgatc gactggggtg gtgggaatcc
     8701 agggattgga gaacgtcgca atgctctctg catcgtccgt cgtttcttca gcgattcgct
     8761 cgaagagcct tgccttggca ctgggagacg gcagggcagg cggggagaga aacggcaaca
     8821 ggtcggtgac acggcgaatc tctgccagcc ctcggcgaca cggcccacac cagagcatgt
     8881 gttgctccat ccgggaacgc tcggtgccct caaccacgtc aagtgcaaac gcctccgcgt
     8941 tttcctgaaa gaatgtgcaa tcagtgttca tggcaccgtg tgaggcatcg ggaccaggat
     9001 gttggtgtgt cattgcagtt caaccccctg atcaacgagg tgctcccgta gttttcgaat
     9061 cgcgaggcgc atgcgcgtct tgatggttcc caacggctcc ccctggagct ccgcgatctc
     9121 tcgctgtgtc agcccgctaa agtaggcgag ttgaatggcg cttcgctggg tctccggcaa
     9181 taaagtcatt gctgttgcaa tttgatcccg aacggttcga aacactgcct gatcctcaac
     9241 cggaacggca ccatccgtca tgttcgtgag gaggagtacg ggatcggcgc tatcggccct
     9301 ctgcgggcga cggttttgtt tccgaatctc atcaatcgcc atgttgtgcg tgatgctcag
     9361 cagccaggtg atgaatgtgc ctttgccctc gctaaacttc cgtgcctgtc gccaggatcg
     9421 gaaaaagacc tcctgcaaca actcctcagc aacggcacgg tctccaagaa gccggagcgc
     9481 gaacgagaac accgctcgtg aataccggtc gtagagaacg tccattgcac gggtatcccc
     9541 cgctgccatg gcgtcgacga gctctgcatc ggccagctcg gcaagattct ccgaaacgcg
     9601 atggtgctga cgcatcactg ggccggaccg tggcaggcgg aatgtgcacc agccgtctga
     9661 caagagttac gtcgaaatgt ggctggggga tgtactggaa gggacattga actcacttgg
     9721 tctgacctgg agcaggtgat ccaccggtgt ggtggcccgg cccaaaggat acagcaccct
     9781 taggtggggg acggatctgg ggatgccagg ccaaggtgaa cgcgggcttc agcggccgct
     9841 gttagcgaac cggtgacaac gactgtgttc cttccgtctt tgagggccaa gtcgagcgct
     9901 gccgcaatgg aaggtgcccc gttaaccctt tggccgagcg tagatagcgc ttcgctcagg
     9961 agatcaaccg gcatcgtccg cggcgaatcc agcctcgcga caatccatcg atcggccctg
    10021 gggatgagcg gcttcaggaa tgggtctggg cgtttgtcct gcagcattcc gatgacgaac
    10081 gtgacgggtt ccgtggaaac atgacgcgtc agtgcagcac tcagtgcgtc cgctgcgatc
    10141 ggggtgtgtg cgccatcgat gatgatcgtc ctgtcttccc ggacgaccct ttcgaaacga
    10201 ccggggtggt gcgcactgcg gaggccggca cgaattgcca aataatccac cgctgaatcg
    10261 tcgctgaagg ccatcttgat cgcagccacg gcgagcgctg cattctcaac ttgatgtggg
    10321 cccacaaggg atgtctgaag tttttcgtcg ctgccccacg gaccggtgac cgcgaacttg
    10381 tcctcgtcac cctccacaaa ccagtcgcga ttctggacga ggagagggct cccgacgtgg
    10441 cgagcccgtt cttggatgac cgacagtgcg gcgtctggct gctcgactgt aaccgagggt
    10501 cgtccgggtt tgatgatgcc ggccttgttc gacgcaatct cagggagcgt ctctcccagg
    10561 atggccgtgt gttcgaagtc gaggcgggtg atcgcggata caaccgggtc gatgacgttg
    10621 gtcgcgtcca gcgttccgcc cagtcctacc tcgatcaccg cgacatcgca catcctttcg
    10681 gcaaaccacg tgagcgccat cgctgtcgaa agctcccagg cggtgatcct gccgagatcc
    10741 ggagtggtcg attctgcctt gcgcactgcc tccataacct gctcagtgag agatgcgaat
    10801 gcgccctcgg tgatgagttc gtcatcgacg atgaaccgct cccgatagga gtggaggtgg
    10861 ggggacgtaa agcggcccga gcgcatgccg gcggcgcgca ggattgcatc gagcaacacc
    10921 gaggtggagc ctttaccctt gctgcccgca acatggacca gttgaatgcg ttcctgcggg
    10981 tggcccagta gatccaggac cgcgctcgtg cgtgccagac ctcgtctcgc ttcgtcgtca
    11041 ccggcgaagg gattatttat gaatccccga tcgtaactgg accggtccca aattgtggcc
    11101 agggcggctc ggtatcgatg ctgttcggat tcgtccacct gtttcaccat tgcatcaagt
    11161 tcctgttttt gcgattgagc attgaagcaa atccgggcac tctccgccta atgctggcat
    11221 cctattggac aacatttaga tggtgacacg agcgtcagca aaaaggagac atcagaacga
    11281 tggaagtcac gaagcacgac atggaggcta ctccttccat tgcaaccaga cgagaagatg
    11341 ttgtcgaagt ccttcacggt caccagattc atgatccata tcgatggttg gaagatggca
    11401 gttcggacga gacccgggcc tggacgcgcg atcagaatgc tcgcacggaa gctgtgcttg
    11461 ccggcatccc cggacgggag caactcgaag ggcagcttcg cgagctgctc caggtaggat
    11521 cggttaccgg tgcaagggag acaggcggcc gcttcttcta tacgcgacgc gagggatcgc
    11581 aggatcaggc ggtgctctat gtccgcgagg ggctcgatgg cgaggagcga acgctcctcg
    11641 acccttcggc gcaaggggag catgggctgg ttgcactcga ttggtgggag ccgtctcacg
    11701 atggcacccg tctcgcctac ggggtctcgc agggcgggga cgaatggagc gtgctccatg
    11761 tgttcgacgt gggcactggc gaggacctgg atgaggccat cccgagagcg agggccgcat
    11821 cggtggcctg gttacctgac aacaacggct tctactacac cagatatccc ctgcctggaa
    11881 gtgtcccgga cggcgagctc cactaccatc gacgtgtatt ttttcacctg atcggatcgg
    11941 attatcacga cgacccacta gtcttcggtg gggaccggcc taaggagctt cttcccgcgg
    12001 tctcgataga gccgactggt cgctgggtca ttgtcgagga gaacgagggc tggatccgga
    12061 caggaatata tgtcctggat cgtcagcatg aggaagctgg cttccgggat atcactcccg
    12121 atggcgctgc gatccatgaa gttcttggga tcgacaacgg ggtgctctac gacctgacgc
    12181 agtggcaggc tccgaatggc cagatcgctg cttattccct ggagaacgga aacgagaagg
    12241 gacaggccgc ctggtcgact gtggttgccg ggcgtgagga ccgcgtgatc gagtacgcgg
    12301 cgatcacgcc cggcggaatc gtcacgggag aactcgaggc agcgatgggc aaggtgcgcc
    12361 gctacggccg tgatgggtcc tcgcggggtg atcttgggct accgggcatc ggttcgatca
    12421 cgggcctcca cgcatccccg tcgagcgata tcgtcgtagt cgggtaccag tcgttcgtgc
    12481 agccgccaac tgcactggtg tatcagggcg atgaccaggc accggtagag ctcagcccag
    12541 tggccccgcc gcccgggttc gaccctgata cctgcgagat ccgccaggtc tggtatgcct
    12601 cgaaggacgg ccagaatatc tcaatgttcc ttgtgcatcg gaaagggctg gagcttgatg
    12661 gctcgaatcc ggtctatctc acggggtacg gtggattcaa cgttacccgt ggttccgagt
    12721 atcaacccgc actgccgttc tggctcggac atggcggcgt ctttgccctg cctaatcttc
    12781 gcggtggtgc ggagtatggt gcggcatggc acgaggcggg gatgcttgct gtcaagcaga
    12841 acaccttcaa cgatttcatt gctgccgcag aatacctgat tgccgagggg tacacacggc
    12901 ctgaacggct gggtatcgcg ggtggaagca acggtgggtt gctcgtggga gccgtggtca
    12961 cgcaacggcc cgaactcttc cgggcagcga tctgcatggt gccgttgctg gacatgcttc
    13021 gttaccatct cttccggatc gcgcgcctct ggatcccgga gtatggatca tcggaagatg
    13081 cggagcagtt cgcatggctc catgcctatt cgccctatca tgcggtccgc gaaggcgtcg
    13141 aatatcccgc catgttgctg acgctgggag aaaacgacag ccgggtcgac cccatgcatg
    13201 cccgaaagat gaccgcgttg cttcagcgcg cgactggcag cggggcggac cggccaatcc
    13261 tgttgcgggc agagagcgac gccggacacg ggcaaggcaa gcccctttgg atgcgagtcc
    13321 aggaggctgc tgacctgtgg ggcttcatga gctggcaact gggcattgac ctggcatagc
    13381 caggggttgc gagcgtcagg ccggagacag ggggtcagag gccgccccct tcataatcat
    13441 ggatataggg attgcgtcag acgccactga acggctctgg cacgcctcgc aggcattcca
    13501 ttgggtgctg aggaccgcgt tccgccatcg tggcgtcgat gtccaccaag cccgcgccgg
    13561 cgacaatcgt gtcctgtaga tgcaggttcg gttcgggtcg tcgtccaggc cgacggaggc
    13621 ggcgataccg ccctgtgctt accacgtatt actctcgccc aactgtccct tgagaaggat
    13681 gacgatccgg agcgaatcag gcaagcgcaa tgcaaacgag agccccgcaa tgccagcgcc
    13741 gacaacgacg atatcggcgt cggttcgcct gtcgcagcaa gctattgggg ctgaaacttc
    13801 tcccactgtc atgccccgcc cggtgcagtc tcggctaatg ggcaacggta aggtccagga
    13861 ggcccgtgtg cgtgatgcgt ccctctggga tgaccgacag ggcgagaaag gcgagctgcc
    13921 cgaacgggtc gtccagcttg cagccgagcg aacgggccgc actcccgagt gcctcgtatg
    13981 tcgccgccac ctcaaataga ggccggtccg acatcagacc cgcgattgga agggggagat
    14041 gcccgagaac ctggccgtct gcgacgacgg ccataccgcc ctgggactcc gcgaccaggg
    14101 cgatggcggc aagcagatca tcgtcggaga cgcccacggc aaggatgtta tgcgaatcgt
    14161 gagagacggt tgtggcgagt gcaccgcgtg ccagaccaaa tccccgcgtg aagccaacgc
    14221 cgatctgacc agtggcatgg tggcgttcga cacagacggt cttcagtagg tcctgagcga
    14281 tatcagaaac agcacctttg tcgcctgttg tcggctgtat gtcgatgaca ctggtgac
//
LOCUS       DASYWM010000065        12644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_77303, whole genome shotgun sequence.
ACCESSION   DASYWM010000065 DASYWM010000000
VERSION     DASYWM010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12644
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77303"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VGR29_02425"
     CDS             <1..544
                     /locus_tag="VGR29_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEV2072474.1"
                     /translation="TVVLITGGATNGFYPVDWSGLKGFMSSDYLTKTSEALSQRGGSA
                     DPGAAPAPTTGSSATGNAMVDYAMRYLGYPYVWATHGPASFDCSGFTYWVAMNVMGKN
                     IGYGTWTQVAAGTPVSRSSLQPGDLVFFQNTYTAGLSHVGLYIGNNKFIHAQNESTGV
                     VISDLDSSYYGPRWYGAVRL"
     gene            609..1481
                     /locus_tag="VGR29_02430"
     CDS             609..1481
                     /locus_tag="VGR29_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2072475.1"
                     /translation="MLIEPAHQRATEGSPAVTDPKLHYLAVNGLQVAWTSVGEASTSP
                     LVFLHGLGDSAIITLRPIATHPAMAGTPALLIDLPGFGYGSATDDWTSSVEEQTEVVI
                     QLLDTVGVVDAPIMGHSMGGSIAIQLATRRPDLVSSLILAEPLLHPDQSVLGRTIAKR
                     TESDFVSRGYAMLQLATRRQAARGERASVGFQEPLGHANPVILHRAASSLLQQRSPSF
                     GEQLEVLANPRTLLVGEMSNLDLGNLVPRDVGIVRIPDAGHSMMSENPDGFARAIAAV
                     LGNYTANTDGMETR"
     gene            1995..4955
                     /locus_tag="VGR29_02435"
     CDS             1995..4955
                     /locus_tag="VGR29_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2072476.1"
                     /translation="MPVGSRSPFGEILRQSRQKRGLSQSALAKLISRRGISDPDFAAL
                     GVISEKAISNLEAARLHPSDYVRPRPQTVQLLTAALGIPSGSPEEEAFLAAAERTRDR
                     NRAKAMPGGDGTHLHFVREGREKQLDHLQGAYEDARNGTPRVVLIAGDPGAGKTSLIT
                     EICQQARQRDRTVIIGWGECTSGATSMEPYLPFFQALNHLLGITVGGSPATASVEADG
                     RHHDAIGMLFTVAPALIGTFVDEPALVAYAQRLSSTNPSLQEQLHRILNIRNATDISG
                     RFDQVVRFITSLTASGPVILVLEDLHWASERAYALLLHLQRHLKDRQDVPLLIIGSYR
                     LSALAHDDGSTRHPLEPVINEVSRQMDTALVDLSTSIGADQGKAFVTSLVHQFSFSPV
                     DEQNIATFLFDRTEGHPLFAVEMLRWLQDSGRLKQHPNGAWELVTTAMDGEVPGKIRA
                     VISERIERLPNHLRRVLEIASVQGNVVSIDVLSQVAGIEPNEMGSLVDNRLVKRYRLL
                     CERDATTINGQRIHLYQFPHSLFQEFLYDSLSTRERERLHIAIAKATIALLGNTSHIA
                     SAPVAFHFAHAHQYREAAEHAVHAGSFAFQQMDYDLALAWYQRAEEYAAKVNDTWRQC
                     DARIGQGLSIRGCSQVDEAIRLAQEILNDARRHGYRDLEARCEDLLGQLYYDDSQFST
                     SAAHLQRSIAINLEIERRVELSGTEAMLSHTLYRLGAYDEALLHAHCAWQTASDFGNE
                     PFEAEALLAAGNCEVDLGMYETALGTYRQARTIYSRSGQVRGEIVCDLNSGLCHLQVG
                     DWMEAIHILTSALEATRTLRTPRLEAVALSYRSLAHEGKGDLGEAEADTRASLELRQR
                     LGQTGLAQDNIATLLRIATARSEERAMRELLGDLETWIGVHGEDGFEDPLHAYLSMAI
                     ACHALGEQDASSRAARAGYQLMMERASRISDDAARRSYLESVPANRMLAEWYRGERAT
                     AMQYSSVECDTPEV"
     gene            complement(5023..6093)
                     /gene="recA"
                     /locus_tag="VGR29_02440"
     CDS             complement(5023..6093)
                     /gene="recA"
                     /locus_tag="VGR29_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV2072477.1"
                     /translation="MAEQTERTRAVDNAIAQIERQFGKGSIMKMVPGAANPMQVDSIP
                     TGSIALDLALGIGGVPRGRIVEIYGPESSGKTTLAMHIIAEAQKAGGMAAIIDAEHAL
                     DPLYAGRLGVNLDDLLISQPDTGEQGLEIAETLVRTGALDVIVIDSVAALVPKAEIEG
                     DMGDSHVGLQARLMSQALRKLTGAINRSKTCVIFINQLRMKIGVMFGSPETTTGGNAL
                     KFYSSVRMDIRRIETIKDGQDATGIRCRVKVIKNKVAPPFRQAEFDIMYNEGISVAGS
                     VLDVATDLGIVRKSGAWFYHGEDRLGQGRENAKTFLKTNQDVLETIRDEIKRASPELS
                     GRLAYDGTEGEGVDADGVLLEK"
     gene            complement(6549..7865)
                     /locus_tag="VGR29_02445"
     CDS             complement(6549..7865)
                     /locus_tag="VGR29_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072478.1"
                     /translation="MFEPGRVALIAKREWSSRVKQRSFQITTIIQVLVVLALGLAPVI
                     IARFAGDDEGSGASVVVVDRADAGVAERLAPWLEGDGDTLETIEVTSGAATPDVARAM
                     VEDGDAQSALIVDRGDDGALTFTLVTEDDRSSRDVQRISAAASGIAMEDRLVQSGVSL
                     EEAQQVFTPVAFNVESPTTGQGNTGFAGSTDANAAEYAIAYISSILMFIAVVMYGTWI
                     AQGVVEEKSSRIMEIMVNAATPRDLLAGKVIGIMMAGLTQLLPMLLVGSLAFSFQTQI
                     ADLMGVNVERVLDIDFGALTVRSVGFFLLYFLLGFVLYGALYAGVGSLVSRQEEVSQA
                     IAPMMTFMMIGYFGAFFVLGAPNTAVARWLMIFPLTSPFSAMPRILLGDPATGEIVLS
                     VVLLLLTATGAIWLAGRLYRVGVLMYGQRPSWRSLVRMGGMQQVAR"
     gene            complement(7888..8844)
                     /locus_tag="VGR29_02450"
     CDS             complement(7888..8844)
                     /locus_tag="VGR29_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2072479.1"
                     /translation="MSLDIHQISKSFGEFTAVQDVSFSVEPGTIFGFLGTNGAGKTTT
                     MRMILDIIRPDTGEVTWNGTPTRDLPRDIFGYLPEERGLYPRMVVEDQLMFLAQLYGA
                     NRKAAKAELDVWLDRLHIEENRRKTVDQLSKGNQQKVQFLASVLHDPQILILDEPFSG
                     LDPVNAEQMKQVFREMRDRGKTIIFSTHQLDDAQELSSDVAIIHRGRLVITGPVGAVR
                     QSTGEHYVRIAVAGDPENLWLEGERDLEIVRRRSDYTEIAVPNEQVARSILREAVRRD
                     LPVTRFEIDYPSLNDVFLTHVRKMPAAMQPTREELTAVPVGD"
     gene            9001..10962
                     /locus_tag="VGR29_02455"
     CDS             9001..10962
                     /locus_tag="VGR29_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV2072480.1"
                     /translation="MPDRSRTETPIIAPYGSWASPFSVDMLTGQTVPLKEPGVDSESI
                     YWLEGRPNEGGRSTLIQLDGAARTELTPAPFDVRSRAHEYGGGSWTAADGTVVFSHGK
                     DNRLYRISPSQPEPSPITPESAYRYADILIDTHHGRIIAVREDHSLEGAEPVNTIVQL
                     ELDGPNEDGGQMIISGSDFVASPALSPDGTRLAWLMWNHPQMPWDGTELWYADVGTDG
                     ALQNIRLVTGGPEESIVRPRWNETGLPVFVSDRTGWWNLYADRTHGIVPLRAMEADFA
                     QPQWVFGMSTWDFLANGSVICAWSQNGVWHLGRLDVEDGPLRRYDLPFTAISDVHVQQ
                     STDTVIFLGASPTDPSGVVRLDANTGEWEMLRRASEAGIDPDTVSIAHPVSWTSTDGE
                     TAYGFYYPPVNPLYTGPEGELPPLIVESHGGPTSFSSSAFSIKRQFWTSRGFAILDVN
                     YGGSTGYGRAYRDRLKGKWGIVDVADCVTGAEMLANQKLVDPARLIIRGGSAGGFTTL
                     AALTSHGTFQVGTSLYGIGDLEAMARETHKFESRYLDGLVGPYPEEKQVYIDRSPIHH
                     VDRLDSAMILLQGLEDKVVPPNQATMMAEAVRANGMPVAHIEFEGEGHGFRSAEAIQR
                     ATEAELSFYAQVLGFTPADDIPVVPVDNL"
     gene            complement(11322..12332)
                     /locus_tag="VGR29_02460"
     CDS             complement(11322..12332)
                     /locus_tag="VGR29_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV2072481.1"
                     /translation="MIRINTASHGMVQGDFDETREVEAAAQTIPYLWRRTQRSSLFRL
                     MQIPRWMYRGYTRTSATDLAASIAYHAMVALVPMFFLLVGVAGLFLRNDTVMRQAIEA
                     IQVVFPAGSGSREAFEAALEARRNSGLISLLSLMGFAWVGTGLISSMARGMNRVYGVR
                     NASYIVEKQRAFVVIFLFLIFFLVAALSSILPTFLLVQELPELLDWLLLSSRFSQIAA
                     YGVAFFSSLMLFLVIYRIVPNARQRFLDVIPGTFAASLLFVMLTQIFPIYIDLVGGVN
                     RYGQLLGLISLIVVALYALAHIILFGSYINATWQRYRQRRRRAQRIQDANQDEPEQVV
                     GG"
     gene            complement(12329..>12644)
                     /locus_tag="VGR29_02465"
     CDS             complement(12329..>12644)
                     /locus_tag="VGR29_02465"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV2072482.1"
                     /translation="GEIVPREMLADRDKVVARVSLTGTHLAEFQGVPPSGKRMIADGT
                     ETFQFRHGVIVESWSLFGPLVEMEKLRSFEQMPSEPKPKRSFLQRLLNRGKHRPEDEE
                     AA"
BASE COUNT         2501 a         4091 c         3671 g         2381 t
ORIGIN      
        1 tacggttgtt ctgatcaccg gcggtgcgac caatggcttc tatccggtcg actggagcgg
       61 tctcaagggc ttcatgagca gcgactacct gacgaagacg tccgaagcgc tctcccagcg
      121 tggtggctcc gccgatccgg gagccgcgcc tgcaccgacg accgggagct cggcgaccgg
      181 taacgcgatg gtagactatg cgatgcgcta cctcggctac ccgtacgtct gggcgacaca
      241 cggcccggcc tccttcgact gctcgggctt cacctactgg gtggccatga acgtcatggg
      301 caaaaacatc ggctatggta cgtggaccca ggtggcggcc gggactccgg tctcgcgcag
      361 cagtctccag ccgggtgacc tggtcttctt ccagaacacc tataccgctg gtctgtcgca
      421 cgtcggcctg tacatcggta ataacaagtt catccacgcg cagaacgagt ccacgggcgt
      481 cgtgatcagc gacctggatt cgagctacta cggtcctcgc tggtacggag cggtccgcct
      541 ctaggctgcc gatccaacca ggacgatctt tcaacacccg ggtgagcctg actctcccgg
      601 gtgttgccat gctcattgag cctgcacatc aacgagcgac ggaagggagc ccagccgtaa
      661 cggacccaaa gcttcactac ctggcggtga atggtctcca ggttgcatgg acctccgttg
      721 gcgaagcgtc cacctccccg ctcgtcttcc tgcatggtct gggcgactcc gccatcatca
      781 cccttcgccc gatcgcgacg catcccgcta tggctggcac ccccgccctg ctgatcgacc
      841 tccctgggtt cgggtacggc tcggcgaccg atgactggac gtcctctgtt gaggagcaaa
      901 cggaggtcgt tatccagctg ctcgatacag tgggcgtcgt agatgcacca atcatggggc
      961 acagcatggg cgggtcgatt gccatccagc tcgccactcg acgaccggat ctcgtttcca
     1021 gcttgatcct tgccgagcca ctactccacc ccgaccagag tgtgctcggc aggacgatcg
     1081 ccaaacgcac cgagtcggat tttgtttcgc gggggtacgc gatgctccag ctggctactc
     1141 gccgtcaggc ggcccgtggt gaaagagcct cggtaggctt ccaggaaccg cttgggcacg
     1201 cgaacccggt catcctgcat cgcgctgcct cctcgttgct tcagcagcgt tcgcccagtt
     1261 ttggggaaca actggaggtc cttgctaatc caaggacctt actcgtcgga gagatgtcga
     1321 atctggattt gggtaacctg gtaccaagag acgtcgggat tgtgcgaatt cccgacgctg
     1381 gccactccat gatgagcgag aatcctgacg gctttgcccg tgccatcgcc gccgtgttgg
     1441 gcaactacac cgcaaacacc gatggcatgg agactcggtg acaagtgtcc atgccatcgg
     1501 cgtgtcatgt attgctgcat gttccgaggt caggcatacc atgcgttcgg acgcctgttg
     1561 gcgcatccgc ttacccgcac gaaagcttat tgaacctgaa tcctcacgag tatcactgca
     1621 ttgaacccac atagggcggc caggtctggc gccgtgaaac ccgtgatcgg actggctaag
     1681 ctgggggacc tccggtccga tcacgagtgc ccgggtctac caccctggcc gtgtatctac
     1741 cttaggccac gcgccccgaa aatccacccc agtatcaccg aagttttcac cgcagtatgg
     1801 gccgcctcgg gccatgcacg acagcgctat taagcctttc gatcttgacc ccgggcagcg
     1861 atcgtagaga ataggatatg ggaggccaca tcccattcgc gaggctcgac cggagcgacg
     1921 agagctccag caactcttgg tccatcaggt cgcaggatca acttttgttc cagcggtcca
     1981 aagcgggagg tgccatgccg gtaggatcac gttcgccatt tggagagatc ctccgccaat
     2041 ctcgccaaaa gcgggggcta tcccagtcag cgctcgccaa gctcatctcc agacgcggaa
     2101 ttagcgatcc tgacttcgcc gcccttggcg ttatctcgga gaaggccatc tccaatctgg
     2161 aagcggcccg cctgcatccg tcagactacg tgcgtccacg cccgcaaaca gtccagctcc
     2221 taaccgcagc cttgggcatc ccttccggca gccccgagga ggaagcattc cttgccgctg
     2281 cggaacggac acgagaccgg aatcgcgcga aggcaatgcc aggtggtgat gggactcacc
     2341 ttcactttgt ccgtgaggga cgcgagaagc agctggacca tctgcaaggg gcctacgagg
     2401 acgcgcgcaa cgggaccccg cgtgttgtgc ttatcgctgg tgatcctggg gcaggcaaaa
     2461 ccagcctgat aaccgagata tgccaacaag cacggcagcg cgacaggacc gtgattatcg
     2521 gttggggcga gtgcacgagt ggcgcaacat cgatggagcc ctacctcccc tttttccagg
     2581 cactgaatca cctgctgggc attaccgttg gcggtagccc tgccactgca agcgtggaag
     2641 ccgatggccg ccaccacgat gcgataggca tgctgttcac tgtcgctcca gccctgatcg
     2701 gcacgttcgt cgatgaacct gcgcttgtcg cttacgctca gcggctcagc tcaacgaatc
     2761 cgtcgcttca ggaacaactc catcggattc tcaacatccg caacgccact gacatctcgg
     2821 gacgcttcga ccaggtcgtg cggttcatta catcgttgac cgcgtctggg cctgtcatcc
     2881 ttgtgctcga agatctgcat tgggctagcg agcgggccta tgccctgcta ctccacctgc
     2941 agcgccatct gaaagacagg caggacgtcc ccctgctgat catcggctcc taccggctaa
     3001 gtgcccttgc gcatgacgac ggttcgacgc gacatccgct cgagcccgtg atcaacgagg
     3061 tgagccgcca gatggacacg gctctcgtcg atctctcgac atccattggt gccgaccagg
     3121 gcaaggcgtt tgtcaccagc cttgttcacc agttctcctt ctcaccggtt gacgagcaga
     3181 acatcgcgac ctttctcttc gatcgtaccg aggggcatcc cctcttcgcg gtggagatgc
     3241 tccggtggct gcaggatagc gggcggctga aacagcaccc caacggtgcc tgggaactgg
     3301 tgaccaccgc catggatggc gaggtaccag ggaagatccg ggcggtgatc tccgagcgaa
     3361 ttgaacgcct cccaaaccat cttcggcgcg tccttgaaat cgcctcggtc cagggcaatg
     3421 ttgtctccat cgatgtcttg agccaggtag caggaatcga gccgaatgag atgggctctc
     3481 tcgttgataa ccggctcgtg aaacgctacc gattgctctg cgagagggac gccaccacga
     3541 tcaacggaca gcggattcat ctctaccagt tcccccactc cctttttcag gagttcctct
     3601 acgactcact gtctactcgg gagcgcgaac gcctccacat tgctatcgca aaggcgacca
     3661 tcgccctact cggcaacacg tcgcacatcg cgtcggcacc ggttgcgttc cattttgcac
     3721 acgcgcacca atacagggag gcagcggaac acgccgtcca tgcggggtca ttcgcctttc
     3781 aacagatgga ttacgatctt gcactcgcct ggtatcagcg ggcggaggaa tacgccgcca
     3841 aggtgaacga tacgtggcgg caatgcgacg cccgcatcgg acagggacta tccatccgcg
     3901 gctgcagcca ggtcgacgag gcaatccgcc tggcccagga gattcttaac gatgcccgca
     3961 ggcatgggta ccgcgatctg gaagcaagat gcgaagacct gctgggtcag ctctactatg
     4021 acgacagcca gttttcgacc tcggcggcac acctgcaacg gtccatcgcc atcaatctcg
     4081 agattgagcg acgcgtcgag ttgagcggca cggaagcgat gctgagccac acgctttacc
     4141 ggctgggcgc ctacgatgaa gccctactcc atgcacactg cgcgtggcaa acggcgtcgg
     4201 acttcggaaa cgagcccttc gaggcggagg ccctgcttgc tgccggtaac tgcgaagtcg
     4261 acttgggaat gtacgagacg gcactgggga cctatcgcca agcccgaacg atctacagcc
     4321 gctcggggca ggtacgagga gagatcgtgt gcgacctcaa ctcggggctg tgtcatctcc
     4381 aggtgggaga ctggatggaa gcgattcata tcctcacgtc cgcacttgag gcaacccgga
     4441 cgctgcgaac accacgcctg gaggctgtgg ccctctccta tcggagcctc gcacacgaag
     4501 gaaagggaga tctcggggaa gccgaagcgg acaccagggc atccctggag ctcaggcagc
     4561 gacttgggca gactggcctg gctcaggaca acatcgcgac actgctacgc atcgccacgg
     4621 cacgatcaga ggaaagggcc atgcgcgagc tgctgggaga tttggagacg tggatcggtg
     4681 tccatggtga agacggcttt gaagacccgc tccatgcgta tctgagcatg gccatcgcct
     4741 gccatgcgct cggcgagcag gatgcctcgt cgcgtgcagc ccgggccgga tatcagctga
     4801 tgatggagcg ggcgagccgt atctccgacg acgcggcgcg gcgctcatat ctcgagtcgg
     4861 tccccgccaa ccgcatgctg gccgagtggt acaggggcga acgggcgaca gcgatgcaat
     4921 actcatctgt cgaatgcgat acgccggagg tatgactggc cctctcctgg cccgccacac
     4981 ctccggcgca tgtcatattt ctgctgtttt caggcgacct ggctacttct ccaggagcac
     5041 accgtctgcg tcaactccct cgccctcggt cccatcatag gcgaggcgac cagaaagctc
     5101 cggtgaggcc cgcttgatct cgtcgcgaat ggtctccagc acgtcctggt tcgtcttgag
     5161 gaaggtcttc gcattctcgc gaccctgccc cagccgatcc tcaccgtgat agaaccaggc
     5221 gccactcttg cgcacgatcc ccaaatcggt tgcgacatcg agcacgctgc ccgcgacaga
     5281 gatgccttcg ttgtacatga tgtcgaactc ggcctgtcgg aacggcggcg cgaccttgtt
     5341 cttgatcacc ttcactcggc atcggatgcc ggtcgcatcc tgaccatcct tgatggtctc
     5401 gatccggcgg atatccatcc gaaccgaaga gtagaacttg agtgcgttac caccggtcgt
     5461 cgtctccggg ctcccgaaca tgacgccgat cttcatgcgt agctggttga tgaagatgac
     5521 acaggtcttg gagcgattga tggcgccggt cagcttgcgg agcgcctggc tcatcagacg
     5581 cgcctgcaga ccgacatggg aatcccccat gtcgccctcg atctccgcct tcggcacgag
     5641 cgccgcgacc gagtcgatca cgatcacgtc caatgcaccg gtgcgcacca gcgtctcggc
     5701 gatctcaagc ccctgctcac cggtgtccgg ctgtgagatc aggagatcgt ccaggttcac
     5761 gcccagcctg ccggcataga gcggatccag cgcgtgctcg gcgtcgatga tggcggccat
     5821 gccgccggcc ttctgggcct cggcgatgat atgcatcgcc agtgtcgtct tgccggacga
     5881 ctccggcccg tagatttcca cgattcggcc acgcggcaca ccgccgatac cgagtgcgag
     5941 gtccagtgcg atgctgccgg tcggaatgct gtccacctgc atcgggttgg ctgcaccagg
     6001 gaccatcttc atgatggatc ccttgccaaa ctggcgctcg atctgggcga tcgcgttatc
     6061 aaccgcgcgc gtgcgttctg tctgttctgc catgtgaacg acttgtcctt cccgaacgcg
     6121 cgtcaccgcg tccgcacctg gcgcctctgc gctctgcctg tctgctgtgt tgctgcccgg
     6181 gcggggatcc tggctcggcc cattctggtg ctctccaccc catcatagca acagccttga
     6241 gcaagtcgaa catctgttcg attacttggt caatagtacc ccatttcgag ataccagtca
     6301 acatggaagc gggttctcca attgggactt tcctcgcgta cgtacgccaa aaacgatggc
     6361 ctacctaaac cggtcctgca cgcctcagac cgtagtcgcc gaaactcgcc tcacggtcac
     6421 tttcaggtat gcaccgggta tcccctagag caatcccgag cgcggtcctt ccatccatca
     6481 cgtccacatc acgaggcggg agagcaagag gctaccttca tgaagctgcc gtgccgattg
     6541 gacagccgct accgcgcgac ctgctgcatg ccgcccatgc gcacaaggct cctccagctc
     6601 ggccgctggc catacatcag cacgcccact cgatagagcc tgcctgccag ccagattgca
     6661 cccgtcgcgg tcaacagcag gagcacaacg ctgagcacga tctcgcctgt cgctggatcg
     6721 ccgagcagaa tgcggggcat tgccgagaag ggcgatgtca gcgggaagat catgagccaa
     6781 cgggctacag ctgtattcgg ggccccgagc acgaagaacg ctccgaaata gccaatcatc
     6841 atgaatgtca tcatcggcgc gattgcctgg ctcacctctt cctggcggct taccagcgag
     6901 ccaacaccgg catagagcgc accgtacagg acaaacccaa gcaggaagta gagcaggaag
     6961 aatccgaccg acctgaccgt gagcgctccg aagtcaatgt ccaggacacg ttcgacgttg
     7021 acgcccatca ggtccgcaat ctgggtctgg aaactgaagg ccaggcttcc caccagcagc
     7081 atcggcagaa gctgcgtcag gcccgccatc atgatgccga tcaccttacc ggccaggaga
     7141 tcccggggcg tcgccgcgtt gaccatgatc tccatgatgc gcgagctttt ctcctccacc
     7201 acgccttggg cgatccatgt cccgtacatg acgacagcga taaacatcag gatcgaggag
     7261 atgtaggcga tcgcatattc cgccgcgttc gcgtccgtcg agccggcgaa accggtgttg
     7321 ccttgtccag tggtcgggct ctccacattg aaagccacgg gggtaaatac ctgctgcgcc
     7381 tcctccagcg acacaccgct ctgaacgagt cggtcctcca tggcgatccc tgaagcggcg
     7441 gcagagattc gctggacatc cctggaggac ctgtcgtctt ctgtgaccag cgtgaacgtg
     7501 agtgctccgt cgtcaccccg gtccacgatc aaggcacttt gggcatcccc gtcttcgacc
     7561 atcgcacggg caacatcagg cgttgcagca ccggacgtta cctcgatggt ttccagcgta
     7621 tctccatctc cctccagcca aggggcaagg cgctcggcaa ctccagcatc ggcacgatcc
     7681 accacgacaa cagaggcacc gctgccttcg tcatcgccgg caaaccgcgc aatgatcact
     7741 ggagccaagc caagggccag caccaccaga acctggatga ttgtggtgat ctggaagctc
     7801 cgctgcttca cgcgcgacga ccattcccgc ttcgcgatca gcgccacccg acctggttca
     7861 aacatgagag ggcatccttc gcttgcttca gtcaccgacc ggcacggccg tcagttcttc
     7921 ccgggttggc tgcatcgcgg ctggcatctt ccgtacgtgc gtcaggaaga catcgttcaa
     7981 tgacgggtaa tcgatctcga accgggtcac agggagatcc cttcgtactg cctcccgcag
     8041 gatcgatcgc gcaacctgct cgttgggcac tgcaatctca gtgtagtcgg accggcgacg
     8101 gacgatttcg agatcccgtt ctccctctaa ccaaaggttc tccgggtctc ccgcgaccgc
     8161 gatgcgcaca tagtgctcac cggtactctg gcgcaccgca ccgaccggcc ctgtgatcac
     8221 cagccggcca cgatgaatga tcgcgacatc gctcgacagc tcctgggcgt catccagctg
     8281 gtgcgtggag aagatgatgg tcttgccccg atcccgcatc tcccgaaaga cctgcttcat
     8341 ctgctcggcg ttcacggggt cgagcccgct gaatggttcg tccaggatca ggatctgcgg
     8401 atcgtgcagc acagaggcca ggaactgaac cttctgctgg ttgcccttgg agagctgatc
     8461 caccgtcttc ctccggttct cctcgatatg gagacggtcc agccacacat cgagctccgc
     8521 tttcgcagcc ttccggttag ccccatagag ctgcgccagg aacatcagct ggtcttctac
     8581 caccatccgg gggtagagcc cgcgctcctc gggaagatag ccaaagatat cgcgcggcag
     8641 atctctcgtc ggtgtcccat tccacgtgac ctcaccggta tctgggcgga tgatatcgag
     8701 gatcatccgc atcgtcgtgg tcttgcccgc gccgttggtg ccgagaaagc cgaagatcgt
     8761 ccccggttcg acggaaaatg agacatcctg caccgcggtg aactcgccga atgactttga
     8821 gatctgatga atatcgagcg acactcgcga gctcctgttc gttggatcaa cgtgcgaaag
     8881 ggcaccatcc gtggcacctc cgattgcctt aagattatgg acgatacttg gatagaacca
     8941 tgtcatgcgc tcccgatccg catgtcgaac gcagtcccat ttccgaaagg atgcaccctc
     9001 gtgccagacc gctcgcgcac cgagaccccg atcatcgccc cgtatggcag ctgggcatcg
     9061 cctttcagcg tcgatatgct gaccggccag acggttccgc tgaaggagcc gggtgtggat
     9121 agcgagtcca tctattggct tgagggccgc cccaacgagg ggggccgctc gacgctgatc
     9181 caacttgatg gcgccgcgcg gactgaactc accccagccc ctttcgatgt ccgctcccgc
     9241 gcccatgagt acggcggcgg ctcgtggacg gcagccgacg gaaccgttgt gttctctcac
     9301 ggcaaggaca accggctcta ccggatatcc ccgtcgcagc cggaaccatc gccgatcacc
     9361 cccgagtcag cctaccgcta tgccgacatc ctgatcgaca cccatcatgg acgtatcatc
     9421 gccgttcgcg aggatcattc cctagaaggg gcggaaccgg tcaataccat cgtccagctt
     9481 gagcttgacg ggccaaacga agacggcggc cagatgatca tctccggcag cgattttgtc
     9541 gcctcccccg ccctctcacc ggacggcacc cggctcgcct ggctcatgtg gaaccatccc
     9601 cagatgccgt gggacggcac cgaactctgg tatgccgatg tcgggaccga tggcgcattg
     9661 cagaacatcc ggttggtcac cggcggcccg gaggaatcca tcgtcaggcc acgctggaac
     9721 gagacgggcc tccccgtctt cgtctccgac cgcaccggct ggtggaacct ctatgccgac
     9781 cggacacacg gcatcgtccc cctaagggca atggaggccg atttcgcgca gccccaatgg
     9841 gtcttcggca tgtcgacctg ggatttcctt gccaatggct cggtgatctg cgcctggagc
     9901 cagaacgggg tctggcacct gggacggctt gatgtcgagg atggccccct cagacgatac
     9961 gacctcccct tcacggccat ctcggacgtc cacgtccagc agagcaccga cacggtgatc
    10021 ttccttggtg cctcccccac cgatcccagt ggtgtcgtcc ggctggacgc caacaccgga
    10081 gaatgggaga tgctgcgcag ggccagcgag gcagggatcg accccgatac cgtctcgatc
    10141 gcgcaccctg tctcttggac atcgaccgat ggcgaaactg cgtacggctt ttactatcca
    10201 ccggtcaacc cgctgtatac ggggccggag ggtgagctcc caccgctgat cgttgaaagc
    10261 cacggagggc ccaccagctt ctcctcgtct gcattcagca taaaaaggca gttctggacc
    10321 agccgtggat tcgcgattct cgacgtgaac tacggtggca gcaccgggta cggccgggcc
    10381 taccgcgatc gactgaaggg caaatggggc atcgtcgacg tggcggactg cgtcaccggc
    10441 gccgagatgc tcgccaacca gaaactcgtc gatccggccc gcctgattat ccggggcggg
    10501 agcgccggtg gcttcaccac cctggcagcc ctcacgtccc acggcacgtt tcaggtcggc
    10561 accagcctgt acggcatcgg tgacctggaa gcgatggcgc gtgagacgca caagtttgaa
    10621 tcacgctacc tggacggcct cgtgggaccc tatccggagg agaagcaggt gtacatcgac
    10681 cgttccccga tacatcatgt cgaccggctc gacagcgcga tgattcttct tcagggactc
    10741 gaggacaagg tcgtgccgcc gaaccaggcg acgatgatgg cggaggcggt gcgagccaac
    10801 ggcatgccgg tcgcccacat cgagttcgag ggcgagggac acggcttccg ctccgctgag
    10861 gcgatccagc gggcaaccga agcggagctc tccttctatg cgcaagtcct cggcttcacc
    10921 ccagccgatg acattccggt cgtccccgtt gacaacctgt aaggagccgg ttcatctgca
    10981 accggctcct cttgggcacc ggtccccctt gcaggcgcct gcgtcagggc tcgtacaaga
    11041 tcgtttgttc cggcatatga gcaggaggca cgcccctgta caaccctccg tcattcagac
    11101 cctgagcaac ctcgaagggg atgaatccaa cgcgaagcga tgcagccaag cgcggcgcag
    11161 ctctgaaaac aggcctttgc ggtcgtgcgg ctcatgaggc gatgtgcaat gcgattgaca
    11221 cggccgccca ctacgtgggg gtgtagcgga cgacgcatag gccattgcag agcaagggag
    11281 cgggagcggc ccattcggcc gagcccggag ggcgagaagc gtcaaccacc cactacctgc
    11341 tccggctcat cctggttcgc atcctgtatc cgttgcgccc gacgccggcg ttgccggtat
    11401 cgttgccagg tggcattgat atagctgcca aacaggatga tgtgcgcaag cgcgtagagt
    11461 gccacgacga tgagcgagat gagcccgagc agctgcccgt accggtttac cccacccacc
    11521 agatcgatgt agatcgggaa gatctgcgtc agcatcacaa agagcaggct ggccgcgaag
    11581 gttcccggga tgacgtctag gaagcgctgc cgcgcattgg gcacaatccg atagatgacc
    11641 aggaagagca tgagagatga gaaaaaggcg accccgtagg cggcaatctg gctgaaacgt
    11701 gagctgagca gcaaccagtc gaggagttcc ggcaattcct ggacgagcag gaacgtcggc
    11761 aggatcgagg atagtgccgc gaccaggaag aagatgagga agaggaaaat aacgacgaac
    11821 gcgcgctgct tctccacgat gtagctcgcg ttccggacgc catagacccg gttcattcca
    11881 cgcgccattg agctgatcag ccccgtcccc acccaggcaa atcccatgag gctgaggagg
    11941 ctgatgaggc cgctgttccg gcgggcctcg agggctgctt caaacgcctc tcgcgacccg
    12001 gatcccgccg ggaagacgac ctggatcgcc tcgatcgcct gccgcatgac ggtgtcgtta
    12061 cgcaggaaaa ggccggccac accaaccagc aggaagaaca tgggcacgag cgccaccatc
    12121 gcatgatagg cgatgcttgc cgcaaggtcg gtcgcgctcg tgcgagtgta gccgcgatac
    12181 atccagcgag gaatctgcat cagccggaac aacgacgagc gctgggtacg ccgccacaga
    12241 tagggaatcg tctgcgcggc tgcctcgacc tcacgcgttt cgtcgaaatc cccctgcacc
    12301 atcccatggg acgcggtgtt gatccggatc atgctgcctc ctcatcctca ggacgatgct
    12361 tgccacggtt caggagtcgc tggaggaagc ttcgcttcgg ctttggttcg ctgggcatct
    12421 gctcgaacga tctcagcttc tccatctcga ccaggggacc gaagagactc cagctctcga
    12481 cgatcacacc gtgccggaac tgaaatgtct ccgtgccatc ggcaatcatc cgcttcccac
    12541 tcggcggcac accctggaac tcggcaagat gcgttccggt cagcgagacg cgcgccacca
    12601 ccttgtcccg atcggcaagc atctccctcg gcacgatttc tcca
//
LOCUS       DASYWM010000066         5693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_377533, whole genome shotgun sequence.
ACCESSION   DASYWM010000066 DASYWM010000000
VERSION     DASYWM010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377533"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1554
                     /locus_tag="VGR29_02470"
     CDS             178..1554
                     /locus_tag="VGR29_02470"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV2072483.1"
                     /translation="MHKTVVLNVVGLVPSLIGESTPRIRAFAERSKSAYIDPVIPAVT
                     ATGHATYLTGKLPAEHGAVGNGWYFRDRDEVKFWRQSNKLVEGPKIWDVAKEMDPTFT
                     CANISWWFNMNSTVDYLLTPRPMYPSDGRKIPDCYTKPMEMRDELQRELGQFPLFNFW
                     GANASIVSSKWIADAAKIFERRFSPTLTLVYLPHLDYCLQKYGPNLTHIARDLREIDE
                     VTGDLIDFYEGTGARVLLVSEYGIMPVSKPVHLNRVFRERGWLSWREELGREMIDVGT
                     CQAFAVADHQIAHIYVNNPAILDEVKQVVEETDGVARVLDEDGKREMGLDHPRSGELV
                     ALAEPDAWFTYYYWVDDAKAPDYARTVDIHQKPGYDPAELFFDPADKYVKARAGLALA
                     RKKLGFRYLMEIVPLDASYVKGSHGIPNEKVEDGPMLISSRPDLLPEEALPATDVFNV
                     MLRHLTEA"
     gene            complement(1793..2638)
                     /locus_tag="VGR29_02475"
     CDS             complement(1793..2638)
                     /locus_tag="VGR29_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072484.1"
                     /translation="MSQQFQMPVLQREMLKEAMFGALTDIPGAVALYVFGSSASGTSD
                     TYSDIDLQLHTTSLRESLSALHRVIHRVGRVQLEWMIHSDERSWAATLLFDAASPYHK
                     IDLGFIEVNPGRPSEGPEQGVLVWKQEPAPAFPARSSVPPYSPNSDSLDYWVIGQLLG
                     MTRYAKARNRQQVATCWRFASSLVNSTMTMWYMHQVSPEVPFHRPLTTHEYLALDRVL
                     SQDQRDRLLGSLDFSSARQMDHAVLDMTGALIDTFRSIVGRSSRSHQTVDLLHSFLLA
                     ELSHP"
     gene            complement(2720..3304)
                     /locus_tag="VGR29_02480"
     CDS             complement(2720..3304)
                     /locus_tag="VGR29_02480"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEV2072485.1"
                     /translation="MAESNIVVATFEDESKTYQAFSEIKHAATEGKLKIQGLTIMHRS
                     LEGRFEVRDAAMKPYGGAVAGGLIGALVGLMGGPLGILLGWGAGSLIGGFRDAKELMS
                     DRTLIQRLTEDMSVGSTALVGEVEETDENLVNQIIRKLGGEVLRRPTAEVEADIAAAE
                     SAQESAAREAHRVMNNDRDTRGQSRPPASDRDGV"
     gene            3446..4057
                     /locus_tag="VGR29_02485"
     CDS             3446..4057
                     /locus_tag="VGR29_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEV2072486.1"
                     /translation="MRWGRKVDPTFLVLFDRAAAFTVEAADALVELFAGDEITQSTFE
                     ALDTIEHRADANTHDLLLRLEKGHIPPLPRAVTRQIALEIDEIVDSAEGAAELAVLSG
                     VRQATPIAREMTVVLAKTTREVASLVSYMGGGTGYRPYVARIHEYEGEGDALWEAGYR
                     SLFNGEIEPLDVLRWKEIYALLEDAIDRCELSAKAIERALGRE"
     gene            4200..4547
                     /locus_tag="VGR29_02490"
     CDS             4200..4547
                     /locus_tag="VGR29_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV2072487.1"
                     /translation="MVADVSVPEEAIAEGDSCCVPDHSESYAADKAKLLARLRRIEGQ
                     VRGIGRMVEDDKYCVDILTQISAVMSSAQSVGLLLLQDHIRGCVVDARPEDREDRLQE
                     LNKAIERFTRSVG"
     gene            4769..5008
                     /locus_tag="VGR29_02495"
     CDS             4769..5008
                     /locus_tag="VGR29_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072488.1"
                     /translation="MGSSADYVQPIASHRKHPERLLLRDDTGQCYLWHGDGREMEAVP
                     ASLAAWICDRPEMHQLQSPLLWFDSSSLPLDTAAF"
     gene            complement(5162..>5693)
                     /locus_tag="VGR29_02500"
     CDS             complement(5162..>5693)
                     /locus_tag="VGR29_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV2072489.1"
                     /translation="ILTFVDTSGADPGISSEERGQATAIAECLMEMIGTRVPIICTVI
                     GEGGSGGALAISLGDRLLMLENAVYAVASPEACAAILWKDASKAAEAAETMRITAADL
                     DRFGIIDELIPEPVPAHEAPRDTVHTVGDRIAAHLDMLCATFDPDDDAGVHDLLDRRY
                     SKFRKIGAWSEAGTEG"
BASE COUNT         1125 a         1678 c         1751 g         1139 t
ORIGIN      
        1 gcctatcgcc agacgaatcg ccaggtcggt ggggcaatcg cgcgcctgat cgccggcatc
       61 tccctggtgg acgcgctggt gctcgcaacc tttgaggcgt ggtggggagt gctcatcgcg
      121 ctggttgcct ttgccgccac cgtctttttc ccacgctata ttcgaggtac ctgatccatg
      181 cacaagacgg tggtcctgaa cgtcgtcggg cttgttccct cgctaatcgg tgagtcgacc
      241 ccgaggatcc gcgcgtttgc tgaacgctcg aagagcgcct acatcgatcc cgtgatcccc
      301 gcggtgaccg ccaccggcca tgcgacctac ctgaccggga agcttccagc ggagcacggc
      361 gcggtgggta acggttggta tttccgggat cgtgacgagg tcaagttctg gcggcaatcc
      421 aacaagctgg tggagggacc caagatctgg gatgtcgcga aggagatgga ccccaccttc
      481 acctgcgcca acatcagctg gtggttcaac atgaactcga cggtcgatta cctgctgacg
      541 ccgcgtccga tgtatccctc ggatggccgc aagatccctg attgctatac caagccgatg
      601 gagatgcgcg acgagctgca acgggagctg ggacagttcc cgctcttcaa cttctgggga
      661 gcgaacgcct cgatcgtctc cagcaagtgg attgccgatg ccgccaagat attcgaacgc
      721 cggttcagcc cgaccctgac gttggtttac ctgccgcatc tcgattattg cctgcagaag
      781 tacggaccca accttaccca tatcgccagg gatttgcggg agatcgatga ggtcaccggc
      841 gacctgatcg acttctacga ggggaccggc gcacgcgtgc tgctggtatc ggagtacggg
      901 atcatgccgg tttcgaagcc ggttcacctg aaccgcgtct ttcgagagcg aggctggctc
      961 tcctggcgcg aggagctggg tcgcgagatg atcgatgtag ggacatgcca ggcctttgcc
     1021 gtcgccgatc accagatcgc ccacatctac gtcaacaatc cggcaatcct cgacgaggtg
     1081 aagcaggtgg tcgaggagac ggatggtgtt gcccgggtgc tggacgaaga cggcaagcgg
     1141 gagatgggcc tggatcaccc ccgttcaggt gagctcgtcg ctctcgcgga gccagatgcc
     1201 tggttcacct attactactg ggtggatgat gcgaaggcac cggattacgc ccgaaccgtc
     1261 gatatccacc agaagcccgg atacgatccg gcggagctct tcttcgatcc ggctgacaaa
     1321 tacgtcaaag ccagggctgg tttggcgctg gcaaggaaga agctcgggtt ccggtatctg
     1381 atggagatcg tgccgctgga tgcgagctat gttaagggct cccacggtat tcccaacgaa
     1441 aaggtggagg acggaccgat gctgatcagc tcgcggcctg atctgctgcc ggaggaggct
     1501 ctccccgcga ccgatgtgtt caatgtcatg ctgcgccacc tcaccgaggc gtaaagcggc
     1561 agacggtccc gatgcgaatg gatgaccaac aagcaggcag tattacagcc agcccgctca
     1621 tccatcaacc gtccatctcg catcctccct caccatgtcg atgatcagcc gggacgactg
     1681 cgcgtcgaca gcccgaaggt gcgactgccg gactgggcct cctcgaaggc acgatcccga
     1741 ccggtcatgg aagacctttc cggtacagcg gtgaccggac ttccccgcgt ctttacggat
     1801 gtgaaagctc cgcaagcagg aaggagtgca ggagatccac cgtctggtga gaacgtgatg
     1861 acctgcccac gattgacctg aacgtatcga tcaaggcacc ggtcatatcc aggacggcgt
     1921 gatccatctg acgagcgctc gaaaaatcca ggctgcccaa caagcgatct cgttgatcct
     1981 gggaaaggac gcggtcgagg gcgaggtact cgtgtgttgt caggggtcgg tgaaatggga
     2041 cttccgggga aacctgatgc atgtaccaca tcgtcatcgt ggagttgacc agggaagatg
     2101 caaaccgcca gcaggttgca acctgctggc ggttccgggc tttggcatac ctcgtcatcc
     2161 cgaggagttg accaatgacc cagtagtcca gcgagtcgga attcggtgag tagggcggta
     2221 ctgaactcct ggccggaaac gcaggcgcag gttcctgttt ccatacgagc acaccttgct
     2281 ccggtccttc ggatggcctt cctgggttaa cctcgatgaa cccaagatcg atcttgtgat
     2341 aggggctggc cgcatcgaaa agcagggtgg cagcccaact ccgctcgtcc gaatgaatca
     2401 tccattccag ttgcactcgt cccacgcgat gaatcacacg gtgtagggcg gagaggctct
     2461 ctcgtaagct cgtggtgtga agttgtaggt cgatatcgga ataagtatcg ctggtgcctg
     2521 atgcgctgga gccgaagacg tagagagcaa cggctcccgg gatatccgtg agcgccccaa
     2581 acatcgcctc tttgagcatt tctcgctgga gcacaggcat ctggaattgt tggctcatga
     2641 tgtgacattg accggcgttc aggatcacaa cgaacggtaa ccatcaggtt tgctcggatt
     2701 ccttggggaa ataatcgggt caaaccccat cgcgatcgct ggcggggggg cgggactggc
     2761 cacgggtatc ccggtcgttg ttcatgaccc ggtgcgcctc gcgcgcggcg gattcctgcg
     2821 cgctctcggc ggcggcaata tcggcctcga cttcggccgt ggggcgccgc aatacttcac
     2881 caccgagctt tcggattatc tggttgacca ggttctcgtc tgtctcctcg acttccccca
     2941 ccaacgcggt gctgcccacg gacatatcct cggtcaaacg ctggatgagc gtgcggtcgg
     3001 acatgagctc cttagcatcg cggaacccgc cgatcagcga tcccgcgccc cagcctagaa
     3061 gaatacctag cgggccgccc atcaacccga caagcgcgcc gatcagacct ccggcgacgg
     3121 caccgccata gggcttcatg gcggcatcac ggacttcgaa ccggccttcc agtgaccggt
     3181 gcatgatcgt caggccctgg attttgagtt ttccttctgt ggcagcgtgc ttgatttcgg
     3241 agaatgcctg gtaggttttg ctctcatcct caaaagtcgc gacgacaatg ttcgattccg
     3301 ccatgacgtg ctggtccttt cggtgcatcc cggatcgcga ggttcggccg ggacgttgat
     3361 gctgttgccg gaactgcgtg tgcgatcatt ttctcacagc atcatcgacg acgcgccgca
     3421 cgcgctggtg tacaggagaa catgcatgag atggggacgt aaggtagatc cgacatttct
     3481 agtcctgttc gatcgtgccg ccgcgttcac cgtagaagca gcggatgcgt tggtcgagct
     3541 ctttgcaggt gacgagatta cgcagtcgac ctttgaagct ctggacacca ttgagcatcg
     3601 tgccgatgcg aatacgcatg atctcctcct tcgcctggag aagggccaca tacccccgtt
     3661 gccaagggca gtgacccggc agatcgccct ggagatcgat gagatcgttg attccgccga
     3721 gggtgcggca gagctggccg tgctttccgg tgttcgtcag gcgacaccaa tcgcccggga
     3781 gatgaccgtc gttctcgcca agacgacgcg agaggtagct tcgctcgtct cctacatggg
     3841 cggtggcacg gggtatcgtc cctacgtcgc acgcattcac gaatatgagg gcgaagggga
     3901 cgcactctgg gaggccggat accgctcgct ctttaacggc gaaattgagc cgctcgatgt
     3961 attgcgatgg aaagagatct acgccctgct tgaggatgcg atcgaccggt gcgagctttc
     4021 ggccaaggcg atcgagcgcg ccctcggcag ggagtaacat atacccccag cccctatctg
     4081 gccatggggc agacgagcgg tcatactggt taagggccgc tagaggatga ctgccggaaa
     4141 tgcgacaggc accctgcatc actgccctga aatgtttccg aggaaaggag cgacctcata
     4201 tggttgctga tgtgtcggtt cccgaagagg ctattgccga gggtgatagc tgttgcgtcc
     4261 cggaccatag cgaatcctac gcggcggaca aggccaaact gctggcccgc ctgcgtcgga
     4321 tcgagggtca ggttcgcggc attggccgga tggtcgagga cgacaagtac tgtgttgata
     4381 tcctgacgca gatatcggcc gtcatgtctt ccgcgcagtc ggttgggctc ctgttgctac
     4441 aggatcacat tcgtggatgt gtggtcgatg cgcgtcccga ggaccgggag gatcgcctgc
     4501 aggaactcaa caaggcgatc gagcggttca cccgcagcgt aggttagctg cgatatcata
     4561 ttgcccatcc tcccgaaggg gctctattgc atccgcggca caacagatgc gataagggaa
     4621 tcgggacaac ttgtgttggc atcgacgggc gcgagatgca cccgattcga tgcacaacgg
     4681 cactctcccg cttgggggct gcaccaccgg tgaatcgtta tggtccgccg agccaccatc
     4741 gggacacaca acgtggaagg agggggcgat gggctcctca gcagactacg tgcagccgat
     4801 cgccagtcac cgtaagcatc ctgagcggtt actgctgcga gatgacacgg gacagtgcta
     4861 cctgtggcat ggcgatggcc gggagatgga agcagtgccc gcgtcattgg cggcctggat
     4921 atgtgaccgc ccggagatgc accaattgca atctcccctg ctctggttcg actcgtcatc
     4981 attgcccctg gataccgcgg cgttctaacc cgaagtatca gatccggcac cacacaagac
     5041 ccctgttggt ccgaaccaac aggggttttg tgtttacagc attcccggcg gtagaccctt
     5101 gtgtctgccc gtcgccggtc ggggaaggac actggaactc ggcttcaccg attagagctc
     5161 gttacccctc cgtgccggct tctgaccagg ccccgatctt ccggaacttg ctgtagcgcc
     5221 ggtcaaggag atcgtggaca cccgcgtcat catcgggatc aaaggtggca cacagcatat
     5281 ccaggtgagc ggcgatgcga tcgccgaccg tgtggaccgt atcccgtggt gcctcgtgag
     5341 ccggaaccgg ttcggggatc agctcatcga tgataccgaa gcgatcaaga tcggcggcgg
     5401 tgatgcgcat cgtctcggcg gcttcggcgg ccttgctcgc gtccttccag aggatggccg
     5461 cgcacgcctc cggcgaggcg acggcgtaga cggcattttc cagcatcaac agccggtcac
     5521 caagactgat cgccagggcc ccgccagacc cgccctcgcc gatcacggtg cagatgatcg
     5581 gcacgcgggt accaatcatc tccatcaggc attccgcgat cgcggtggcc tggccgcgtt
     5641 cctccgagct gatgcccgga tcggcccccg aagtgtcgac aaaggtcagg atc
//
LOCUS       DASYWM010000067        13026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_415301, whole genome shotgun sequence.
ACCESSION   DASYWM010000067 DASYWM010000000
VERSION     DASYWM010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13026
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415301"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGR29_02505"
     CDS             complement(<1..224)
                     /locus_tag="VGR29_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072490.1"
                     /translation="MTSLKISASSKEGNVQLELEEQGPEMPPWDAATSLDIVGKGTSR
                     LEAFEKVTGHAQYSSDVRLTRQSYAKVLRSP"
     gene            complement(221..721)
                     /locus_tag="VGR29_02510"
     CDS             complement(221..721)
                     /locus_tag="VGR29_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV2072491.1"
                     /translation="MSSTHVPIAISINGETHQLMIEPRRTLLDTLRHDLRLTGAKKVC
                     DMGNCGACTVTVDGQAMYSCLLLAVDCDGREIRTIEGLATGEKLDPVQQAFVEEDAFQ
                     CGYCTPGQIMSIRALLDDHPQPTDEQIMRAVSGNLCRCGAYQHIARAGRRAAELQAAG
                     SHGATS"
     gene            904..1446
                     /locus_tag="VGR29_02515"
     CDS             904..1446
                     /locus_tag="VGR29_02515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2072492.1"
                     /translation="MTPRDANELVICGENAASVEIDDARLHDLRAVAAIQRASFRPGL
                     AYGMFPLVTLRIMPFVTFLVVRKSKTGEVMGCIIADRYRGDVRIMNIAVHPEWRRRGV
                     ATALLRALDERLPGGNIVLMVEETNHGAQALYQEDGFTRSGYQRNYYGSHRHGIEMTR
                     QRQPPSAVRSGKPTSGHIRV"
     gene            1977..2315
                     /locus_tag="VGR29_02520"
     CDS             1977..2315
                     /locus_tag="VGR29_02520"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV2072493.1"
                     /translation="MTRYLISFDDGAMTFPKEDLPDVAEASHKVVYEAKDAGVWIFGG
                     GLLGQSASIVATDGTVTNGPYPETKAVIGGFSIIDVPSREEALEWAAKIAVACRCAQE
                     VREIMYDPDS"
     gene            complement(2382..2741)
                     /locus_tag="VGR29_02525"
     CDS             complement(2382..2741)
                     /locus_tag="VGR29_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072494.1"
                     /translation="MTRNHHEAELPETPFDEATDLVGPEEDVPEPSGRRFVLPSWKPL
                     IFILAVMLAPVFGLAFGLDFALGVLVVAMAFTTWMAWEGSRELPHDEGSSLRRAAMLN
                     GALALAVLALLIGRQFV"
     gene            3141..3947
                     /locus_tag="VGR29_02530"
     CDS             3141..3947
                     /locus_tag="VGR29_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2072495.1"
                     /translation="MPDTSPSTLREWNASSYHLVANPHVDWGKAVLERLPLRGDETVI
                     DAGCGTGRLTELLLERLPQGRVIAVDQSANMLERAEQHLRPHFGDRVSFARHDLVDLP
                     HEQIADAVFSTATFHWIPDHPRLFATLFRALKPGGWLIAQCGGARNIQTIDERARAIL
                     SEAPFGEHIGDWKGPWNFADAPETEARLTEAGFTGTVAHIIEAPVVLGGEAEYREFLE
                     TVVLGSHLMRLPNSSLRRTFLDRMVADGTADDPPWSLPYTRLNLQGQRPR"
     gene            complement(3944..4702)
                     /locus_tag="VGR29_02535"
     CDS             complement(3944..4702)
                     /locus_tag="VGR29_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV2072496.1"
                     /translation="MFDERGLEFFDLMLRLGMATVLGAAIGFERERQERAAGFRTHAL
                     VCVASALMMIVSTYGFPEEFGEVLDPARIAAQVVSGIGFLGAGVIIFRKNTVRGLTTA
                     ASVWAVSGIGLAVGGGLFVAGTAGALFMLLIQGGLRPVERKLFAHHSRRHRVLLTVPH
                     TTVVFTDIQRIAANKPGFRLMSLDFDLDEKAKHDIVELTLEVKHPDDMLELLSQFRQI
                     SGLVHLVWREGPANVARRNRRGVELTVDDDDENS"
     gene            complement(4868..6316)
                     /locus_tag="VGR29_02540"
     CDS             complement(4868..6316)
                     /locus_tag="VGR29_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEV2072497.1"
                     /translation="MNRLISNQKYVIAVVYVSTMLLNTLDATIVNVALATLSREFNVS
                     PASIESVVIGYIVSLAVFMPASGWLGDRFGAKRIFLLALSLFTVASALCGVAQSLDQL
                     VLFRVLQGAGGGLLTPVGMAMLYRAFPPEERVGVGRILMFATILGPALGPVIGGLILE
                     YFSWRWCFYVNIPIGVAALVFGLLFLHDHRQEDVGSFDLPGFLLAGAGFGLTMFALSE
                     GPQEGWLSPLIASCGVVGLVLLAIFVVVELRTRQPMVQLRLLSNRLFRAIIRVSIFGT
                     AGFLGALFLLPLFLQEVRGESALTAGLTTMPEAIGVVLATQVVARIYPLIGPRRLMAS
                     GLVLVAISVALLGMVGRETPLWVVQVVMFFVGVGMAFVFLPNQAASMATISRTDTGGA
                     SMLFSVQRQLGSAMGVALLSTVLATIGVFRATADGGREPNLTAYHAAFFVAAGLSLIG
                     AVMALFVPDEDAAATMRRRPVAEKREPVEAVA"
     gene            complement(6637..7881)
                     /locus_tag="VGR29_02545"
     CDS             complement(6637..7881)
                     /locus_tag="VGR29_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV2072498.1"
                     /translation="MNETNWAGNYGYRATRLHRPETMDELRKIVAEAPKIHALGSRHS
                     FNGIADAGELVSLERLEQRIEIDRAAGTVTVNSGIPYGALAQILEHEGLALHNMASLP
                     QISVAGAISTATHGSGDANGNLSTAVTALELVTSDGGLLRAARDDDDFAGMVVGLGAL
                     GVITRLTLKVEPSYLVRQEVFEHLEWDVFFNRFDEVTSSAGSVSLFTDFSESVNQVWL
                     KSRVHPDHAEPLRDDLMGAVPATRMLHPVGTLSPDNCTQQLGIAGSWADRIPHFRMDA
                     PPASGDEVQAEYMVSRRHAVQALQALRQVGDAIRPHLWISEIRTVAADDLWLSTAYGV
                     DTVAIHFSWKNDVEAVARVLPIVEEALVPFEPRPHWGKLFMISGSDIASRYEKMPDFR
                     RLAQRLDPRGAFRSEFLEGVLG"
     gene            8001..8489
                     /locus_tag="VGR29_02550"
     CDS             8001..8489
                     /locus_tag="VGR29_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2072499.1"
                     /translation="MPLTDEIRTRLLELLGRGNSHMAFDDAVRDFPMDRINETPPNVP
                     YSPWYLLEHMRLTQRDILDYIEQEDYRERAWPDDSWPPPGTTADEAAWNETIRQFHED
                     LTSLEALVSDKSRDLEGTVPSNDRHTILREIITVASHNHYHIGEFAILRQVMGTWPPG
                     HR"
     gene            complement(8528..9415)
                     /locus_tag="VGR29_02555"
     CDS             complement(8528..9415)
                     /locus_tag="VGR29_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072500.1"
                     /translation="MMAEERVAPLVISRRQGFLITFVQRNHIASVAASILVLIVVSAI
                     IAPVLAPGDPVDMSPIDRLKSPADAGYLGTDAFGRDILSRILYGARISLGVGVVSVLA
                     AAAVGTLIGLVSGYLGGWQEYLTMRLMDVLFSFPSILLAILIVSIWGAGFSSIVLAIA
                     LVQTPIFARIVRASTLSLKEMEYVHAAKTAGASTWRILSRHVLPNVAAPILVQLALSM
                     SGAVVLEAALSYLGLGAVPPTPSLGSMLSENRSAMEIAPWTVLYPGIALAILVLCINM
                     LGDALRDFLDPRLRTAMSP"
     gene            complement(9421..10377)
                     /locus_tag="VGR29_02560"
     CDS             complement(9421..10377)
                     /locus_tag="VGR29_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072501.1"
                     /translation="MLSFLARRIITVFIPTLLGISILVFGAMRQIPGDFVDVLIGVGP
                     DVSEQQREDIARSYGLDKPLPVQYVLWLGNVLQGDMGTSLRSGKPVASEIIGRLPVTL
                     ELAGLATIASLVLAIPAGIVSALRRGRMSDSVLRLVALVGLSVPNFLIATMLVLFVST
                     KWSFFPTTGFVPITEGIGANLKSLVLPTLALGALLAASVMRMTRSSVLEELGKEYLTV
                     ARAKGLAERAIVLRHAVRNALVPVITVVGIQTGYMLGGTVIIEQIFAIPGIGRLALDA
                     VNQRDYPLVQGTTLFIAAAFVLMNLVTDVIYSVVDPRIRVKG"
     gene            complement(10555..12120)
                     /locus_tag="VGR29_02565"
     CDS             complement(10555..12120)
                     /locus_tag="VGR29_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072502.1"
                     /translation="MDRWSGNRRQFTKVAGGAFTVATVGGHLAANRAIAQDASPAASS
                     AAGELIIGKSEEAVGLDPAMVTAASSFDLIAAVYETLVIFDEDGQPQPWLAESWETPD
                     DLTYVFTLLEGVTFHNGQPLTADDVVFTFERMMDPESGSPWTSQFAPVESVEATDERT
                     TTFRLKETYGPFLASLSSNYAAIVPRADGVDLQTTMVGTGAFALLERAEDVETTLEAN
                     PDYWMDGLPKLANVTYRILPDESARVAAIRTGEIHLTALADPISIETARSSAGITLIE
                     QETTDYYLLGFNCAVAPFDDPKVRQALSMAVDRQAIIESVFFGQGQVSGPLVPTLGDW
                     ANPIEELPNYSVDRDAAKALLEEAGQGNLTFKITVGANRQEFVNIALVIQDQLKEIGV
                     TVELDQVEWGTFIDKWIARDFESFVSYNGSGNDPDRALYPALYTDGSVNAFQFSDPKV
                     DELLDRGRELTTHEERKPVYQELEIVVAEHAPLLFLCTRVANFATTQSVEGFAPSASQ
                     TWMTLPETTVNGE"
     gene            12388..12819
                     /locus_tag="VGR29_02570"
     CDS             12388..12819
                     /locus_tag="VGR29_02570"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEV2072503.1"
                     /translation="MVQKTAPNMVKMEESELTLANSDEDIRGYKVVDSAGEDIGDVKD
                     LLIDEDEAKVRFLQVGSGGFLGIGDETFLIPVDAITHIDDNAVRVNLTRDRVRQGPTY
                     DPTMVRDDEYWNTAYSHYGFLPFWAPGYRYPTFPRYPRHRR"
BASE COUNT         2738 a         4346 c         3709 g         2233 t
ORIGIN      
        1 gggctccgca gcaccttcgc gtagctctgt cgcgtcagtc gcacgtcgga gctgtactgg
       61 gcgtggccag tcaccttctc gaacgcttcc agccgggagg ttcccttgcc aacaatatcg
      121 aggctggtgg ccgcgtccca cggcggcatc tccggcccct gctcctccaa ctcgagctgc
      181 acgttgccct ctttggaaga ggcgctgatc ttcagtgagg tcatgatgtc gctccatgcg
      241 agccagccgc ctggagctcg gccgcacgtc gcccggcccg cgcaatatgc tggtaggcgc
      301 cgcaacggca gagattgccg ctgaccgccc gcatgatctg ctcatcggtc ggttgcggat
      361 gatcgtccag cagcgcgcgg atgctcatga tctgccccgg cgtgcaataa ccgcactgga
      421 aggcatcctc ctcaacaaac gcctgctgga ccgggtcgag cttctcgccg gtggcgagtc
      481 cctcaatggt ccggatctcc cgcccgtcgc aatcgaccgc gagcaagagg caggaataca
      541 tcgcctgacc gtccacggtc accgtacatg cgccgcagtt gcccatatcg cacaccttct
      601 tcgcaccggt cagtcgcagg tcatgccgga gggtgtccaa caaggtcctg cgaggctcga
      661 tcatcaactg gtgcgtctcc ccattgatgg agattgcaat cggaacgtgt gtgctgctca
      721 ccaggacctc ctcagatgcc aatgtaagga cgccctcatg gggcgccggg ctttggcacg
      781 ctgttcgcat gggaggtgcc agcgcgggta gaagccgcgg cgggaggtca ggtaaatgcg
      841 ctatcctcag gtaaagagca tgcaccgcct gcatgacgac gatgcaccga caggaaatcc
      901 cccgtgaccc cgcgtgatgc caacgagctc gtgatctgtg gtgagaacgc cgccagcgtt
      961 gagatcgacg atgcccgcct gcacgatctc cgcgccgtcg ctgcaatcca gcgggcatcc
     1021 ttccgtcccg gcctcgccta cggaatgttc ccgctcgtaa ccctgaggat catgcctttc
     1081 gtcacctttc tggtcgtaag gaagagcaag accggggagg ttatgggctg tatcatcgcg
     1141 gacaggtacc ggggcgatgt gcgtatcatg aacatcgccg tgcacccgga atggcgtcgg
     1201 cggggtgttg caaccgcact gctccgggcc ctcgacgagc gcctgccggg cggaaacatc
     1261 gtgctgatgg ttgaggagac caaccacggc gcccaggccc tgtatcagga agacggcttc
     1321 acccgaagcg gatatcagcg gaactactat ggttcccacc ggcacggcat cgagatgaca
     1381 cgccagcgcc agccacccag cgctgtccgc agcggcaaac caaccagcgg ccacatccgt
     1441 gtatgacgct ctctcttgga catgttcgtc atcctgaacg cacggccgca tccgtgtgtg
     1501 acgccctctc ttgtaaatat ttatcattct gaacgcagtg aagaatcccc gagcgaagcg
     1561 cgttggcacc gcaggtcgcg atcctcatgc cggtagtcat tccaccctcg gcgcgtacac
     1621 agacctggag cgtgttatgg actcgtgcgt ggaggtaggc tttggaaatg agtagtcgcc
     1681 gcaggctgta ctccagcgac gtgactgagg agtgggcgtt cgtcgccccg cagctggtcg
     1741 cgctggtccg cgcgggcgca acatccgaga aacggcaaga tcgtcgagcg agccgacgac
     1801 tcgggaggtg acgcccaaac ttcatggcac gtagatcgac ggatctcgtc ttatcgtagt
     1861 atcgaggtcg gtgtcgctct ctagcgccac cgtggtcggt agcctgtcgg ttccggagcg
     1921 aggcgttcgt cgagggaaaa agccggccgg aggaggccgg gacaaggagc cacgagttga
     1981 cgcgatatct gatctcgttc gatgacgggg cgatgacctt ccccaaggag gacttgcccg
     2041 acgtggccga ggcctcgcac aaggtggttt atgaggccaa ggacgccggg gtgtggattt
     2101 tcgggggcgg actgctaggc cagagcgcga gcatcgtagc caccgacggg acagtcacca
     2161 atggcccata cccggagacc aaggcggtca tcggcgggtt ctcgatcatc gacgttccct
     2221 cacgcgagga ggcgctggag tgggcggcca agatcgccgt cgcctgccgg tgtgcgcaag
     2281 aggtacggga gatcatgtac gacccggact cctgattgat cggcggcccc cgcgtccggt
     2341 ccatgccccc ttgccacccc acggaagttc ataaacacgc tctagacgaa ctgccgcccg
     2401 atcagcaagg ccagcacggc cagggcgagg gccccattga gcatcgccgc tcgccgtagt
     2461 gagcttcctt catcatgggg cagctctctc gacccctccc atgccatcca ggtcgtgaac
     2521 gccatcgcca ccaccagcac accgagcgcg aagtccagcc cgaacgcaag cccgaacacc
     2581 ggcgccagca tcaccgccag gataaagatg agcggcttcc acgaaggcaa cacgaaccgg
     2641 cgcccggacg gctcagggac atcttcttcc ggtccgacga ggtcggttgc ctcgtcaaac
     2701 ggcgtctcgg gaagttcggc ctcgtgatgg tttcgggtca tcggtcgcct gttctcttgc
     2761 gtcacatcct catgtcgcag atgctaccag agatcgacgt ggtggctgtt ccaaaagaca
     2821 agcgatccgc caaacaaact ccgtcattca gaggcaccag ttcaatcagt cctacacacc
     2881 aaaccaccgt cattcagacc ctgagcaacg tcgaagggga agaatcctgc gcggagcggt
     2941 gtagcgagga ctgtgcacac gtttgttccc gcacgttcga ggtcacccgt tctcttgtgc
     3001 cacatctcca tgtcggagat gctaggggtc gagggcacgg agatctgtac gggcgaactc
     3061 tatgttcgcc cattacatgg accaacaccc tgcacaggcg cataatcgaa ggcactccac
     3121 ccgacaacaa gacattcccc atgcctgata ccagcccatc caccctccgc gagtggaacg
     3181 cgtccagtta tcacctcgtc gctaacccgc acgtcgactg gggcaaagcc gtccttgaac
     3241 gcctgcccct gcgcggcgac gagaccgtca tcgatgctgg ctgcggcacc ggccgcctga
     3301 cagagctcct gctggagcgc ctgccacagg ggcgcgtcat cgctgtcgac cagtcagcca
     3361 acatgctgga gcgggccgag cagcacctcc ggccccactt tggggatcgc gtctcctttg
     3421 cgcggcatga tctcgtggac ctgccacatg agcagatcgc cgatgccgtc ttcagcaccg
     3481 cgaccttcca ctggattccc gatcaccccc gcctcttcgc caccctcttc cgcgcgctca
     3541 agccgggcgg ctggctcatc gcccaatgcg gcggcgcgag gaacatccag accatcgatg
     3601 agcgggcaag ggccatcctc agcgaggctc cgttcgggga acatatcggc gactggaaag
     3661 gtccctggaa cttcgcggac gcccccgaga ccgaggctcg tctcaccgag gccgggttca
     3721 cgggcactgt cgcccatatc attgaagcgc ccgtcgtcct cgggggcgaa gcggagtacc
     3781 gcgagttcct ggaaaccgtt gtcttgggga gccatctaat gcgcctgccg aactcatctc
     3841 tccgtcgcac attcctggat cggatggtgg ccgacggcac tgccgacgac ccgccttggt
     3901 cgcttcccta cacccgcctc aacctgcagg gacaacgacc gcgctagctg ttctcgtcgt
     3961 catcgtcaac cgtcaactcc acgccacgcc ggttacgccg ggcgacattc gccggtccct
     4021 cgcgccacac aagatgcacc aggccactga tctggcggaa ctggctcagc agctccagca
     4081 tgtcgtccgg gtgcttgacc tccagtgtga gctccacgat atcgtgttta gctttctcat
     4141 ccagatcaaa atcgagcgac atcagccgga accccggctt gttggcggca attcgctgga
     4201 tatccgtgaa cacgacggtg gtatgcggca ccgtgagcag cacccggtgg cggcgcgaat
     4261 ggtgtgcgaa caactttcgc tccaccggcc gcagcccccc ctggatcagc agcatgaaga
     4321 gcgcccccgc ggtgcccgcc acaaaaagcc caccgccaac ggcaagccca atcccggaaa
     4381 ccgcccacac actggccgcc gttgtcaggc cccgcaccgt gttcttgcga aagatgatga
     4441 cacctgctcc caggaaaccg ataccgctga cgacctgagc cgcaatacgc gctggatcca
     4501 gaacctcacc gaactcctcc gggaacccgt aggtcgacac aatcatcatc agcgccgacg
     4561 caacacagac gagagcgtgc gttcggaatc cggcggcccg ctcctggcgc tcccgctcga
     4621 acccgatcgc cgccccaagg acggtcgcca tacctagccg cagcatcaga tcgaagaatt
     4681 caagcccgcg ctcatcaaac atactccagt caaaatcgaa cacctgtccc cctcgttcca
     4741 atagcgcgat gccaattatg aacaaaatgt taacgccgca tcgtccagca acataccact
     4801 attcagcatc cctacccggt ccagcgccgc atcagcagaa cgaaacgacc ctttcatggc
     4861 tggatcgcta tgccacggcc tccacgggct cccgtttctc ggccaccggt cgtcgtcgca
     4921 tcgtcgcggc ggcgtcctca tcggggacga acaacgccat gacagcgccg atcaatgaca
     4981 acccggcagc cacgaaaaat gcggcatgat aagcagtcag gttcggctcc cgtccaccat
     5041 cggccgtcgc ccggaatacc ccgatggtcg cgaggacggt gctcagaagt gcgacgccca
     5101 tcgcggagcc aagctgacgc tgcacgctga agagcatcga cgcgccgccg gtgtccgtcc
     5161 gggagatcgt cgccatgctt gcggcctggt tggggagaaa gacaaacgcc atcccgaccc
     5221 cgacgaagaa catcacgacc tggacgaccc agagcggcgt ctccctccct accatcccca
     5281 gcagtgccac gctgatcgcg accagcacca gcccggaggc catcagccgc cgcggaccga
     5341 tgaggggata gattcgcgcc acgacctggg ttgccagcac cacgccgatc gcttccggca
     5401 tcgttgtcag gcccgctgtc agcgccgact ccccgcgcac ttcctgcagg aagagcggca
     5461 gcaggaagag tgcgcccagg aatcccgccg tgccgaagat ggagacccgg atgatcgctc
     5521 ggaaaagccg gttggagagc aggcgcaact gcaccatcgg ctgccgggtg cgcagctcca
     5581 cgacgacaaa gatcgccagg agcaccagtc ccaccacccc acaggacgcg atcagcggcg
     5641 acagccatcc ctcctgcggc ccctcgctca gggcgaacat tgtcagcccg aacccggcgc
     5701 cagccagcag gaagccggga agatcgaacg accccacatc ttcctgccgg tgatcgtgaa
     5761 gaaagagcag accgaagacc agtgccgcca ccccgatcgg gatgttcacg taaaagcacc
     5821 agcgccagga gaaatactcc aggatcaggc caccaatcac cggccccagc gccggaccca
     5881 ggatcgtcgc gaacatcagg attcgcccga cgcccacccg ctcctccggc ggaaaggcgc
     5941 ggtagagcat ggccatcccc accggcgtca acaagccgcc gcctgccccc tgcagcacgc
     6001 gaaagagcac caactggtcc aggctctgcg ccaccccgca cagggccgac gccaccgtga
     6061 agagggacaa cgccagcagg aagatccgct tggccccaaa ccggtcgccc aaccaccccg
     6121 atgccggcat gaagaccgcc agactgacga tatagccgat cacgacagat tcaatcgatg
     6181 ccggagagac gttgaactcc cggctaagcg tcgccagtgc gacattgacg atcgtcgcat
     6241 cgagggtgtt cagcagcatc gtgctgacat acaccacggc aatcacgtat ttctggttcg
     6301 agatcaagcg gttcacaaag aagctatccg attggtgaga tatcgatggc aaagcgggca
     6361 tgcgacgaga ccggcggtaa ctgccggagt ggaaggatcc cgggcgcgat gccctagtat
     6421 accgataccg ttgtgcgcgt cagcggccac ttaattgccg cgcatgcgag cgatcaacac
     6481 ccgtgtcatc ccgggcccgg tcgagggatc tcccgatgcc gcgcaggggt cgactccatg
     6541 tcgacccgat accgcgccag ggtgttcccg aacccggccc tgcgccggct gctgcaccgt
     6601 gtatccgctg tccgtatcag tcccgttcac atacccctac cccagcacgc cctccaaaaa
     6661 ctcactccgg aaggcccccc ttgggtccag ccgttgtgcg aggcgtcgga agtccggcat
     6721 cttctcatac cgggatgcga tgtcgctccc gctgatcatg aacagcttgc cccagtgtgg
     6781 cctcggttca aaggggacaa gtgcctcctc tacaatcggc aggacacggg caacggcttc
     6841 cacgtcgttc ttccagctga aatgaatggc taccgtatcg actccgtagg ccgtgctcag
     6901 ccagagatca tcggcggcca ccgtacggat ctccgagatc cacagatgcg gccggatcgc
     6961 atcgcccacc tgtctcaggg cctgcagtgc ctggacagcg tgacggcggg aaaccatgta
     7021 ctccgcctgg acctcatccc cacttgccgg gggcgcatcc atccggaagt gcgggatacg
     7081 gtcggcccag gacccggcta tcccgagctg ctgcgtgcaa ttgtcggggg agagcgtccc
     7141 cacaggatgc agcatcctcg tggcggggac cgcgcccatc aggtcatcac gcagcggctc
     7201 cgcatgatcg ggatggacac gactcttgag ccagacctgg ttcacactct cactgaagtc
     7261 cgtaaagagg ctgacactcc cggcgctcga ggtgacctca tcgaagcggt tgaagaacac
     7321 atcccactcc agatgctcga agacctcctg gcgaaccaga tagcttggct ccaccttcaa
     7381 cgtgagacgc gtgatgaccc cgagagcacc aagcccgacg accatcccgg caaaatcgtc
     7441 atcgtcacgt gcggcccgca gcaggccgcc atcgctggtc accaactcca gggccgttac
     7501 cgcagtcgac aggttgccat tggcgtcgcc cgagccgtgg gttgccgtgg agatcgcacc
     7561 cgcaaccgag atctggggca acgacgccat gttgtgaagc gccagcccct cgtgctcgag
     7621 gatctgggcc agggcaccat aggggatgcc actattcacc gtgacggtcc ccgccgcccg
     7681 gtcgatctcg atccgctgct ccagccgttc cagcgagacc agctctccgg catccgcgat
     7741 accgttgaat gagtgacgcg aaccaagcgc gtggatcttc ggcgcctccg ccacgatctt
     7801 gcgcagttcg tccatcgttt ccggccggtg cagccgcgtc gcgcggtagc catagttccc
     7861 ggcccagttg gtctcattca tcgacacacc cttttccaga ccattcaaca gcaatggtat
     7921 ggcggtcatc atcgcacgat gtcatcatca caccatcatg acaccgcttt ggcacgacgc
     7981 atccaccgag aggagacacg atgcccctga ctgacgagat tcgcacgcgg ttgttagaac
     8041 tgctggggag aggcaactcc catatggctt tcgacgacgc ggtcagggac tttccgatgg
     8101 accgcatcaa cgaaacccca ccgaatgtcc cctactcccc ctggtatctc ctggagcaca
     8161 tgcgactcac ccagcgggac atcctcgact acatcgagca ggaggactac cgggaacgcg
     8221 cctggcctga tgactcgtgg ccaccacccg gcaccaccgc ggacgaggcc gcctggaacg
     8281 agaccatccg tcagttccac gaggatctca catccctcga agcgctcgtc tccgacaaat
     8341 cccgggacct ggaagggacc gtcccgagca acgaccggca caccatcctg cgcgagatca
     8401 tcactgtcgc tagccacaat cactatcaca tcggcgagtt cgcgattctc cgccaggtca
     8461 tgggtacctg gcccccgggt caccgctaga ggcgccggct cgatggacac tcgaagccgc
     8521 cccaaaatca ggggctcatc gccgttcgca ggcgtgggtc cagaaagtcc cgcagggcat
     8581 caccgagcat gttgatgcag agcacgagaa tcgccagtgc gatgccagga tagagcaccg
     8641 tccaaggcgc gatctccatg gcgctacgat tctcactcag catcgatccg agcgagggcg
     8701 ttggcggcac cgcccccaac cccagatagc tcagcgccgc ctccagcacc accgccccgg
     8761 acatgctcaa ggcgagctgc accaggatcg gggcggcgac attcggcaac acgtgacggc
     8821 tcaagatccg ccacgtcgaa gcaccggccg tttttgccgc gtggacgtat tccatttcct
     8881 tcaacgacag tgtggaggcg cgtacgatcc gggcaaagat cggcgtctgc accagcgcga
     8941 tcgcaaggac gatgctggaa aagcccgcgc cccagatcga cacgatcagg atcgccagca
     9001 agatcgacgg aaaggaaaaa aggacatcca tgagacgcat ggtcaggtat tcctgccaac
     9061 cgccaagata ccctgaaacc aggccgatca gggtgccgac cgctgcggcg gcaagtaccg
     9121 aaacaacccc gaccccaagc gaaatacgag ccccatagag aatccggctc aggatatccc
     9181 gcccgaaggc gtccgttcct agatatccgg catcggctgg tgacttaagg cgatcgatcg
     9241 gcgacatgtc caccggatcg cccggtgcga gcaccggcgc aatgatcgcg ctgacgacaa
     9301 tcaacaccag aatggacgcg gccacgctcg caatatgatt tctctggaca aaggtgatca
     9361 ggaatccctg ccgccgtgat atcacgagcg gcgcgacgcg ctcttcagcc atcatcccgc
     9421 tcatcccttc acccggatac gcggatccac cacactgtag atcacatcgg tcaccagatt
     9481 catcagcacg aaggcagcag caatgaagag cgtcgtcccc tgcacgagcg ggtaatcccg
     9541 ttggtttacc gcgtcgagcg ccagccgtcc aatgcccgga atggcgaaga tctgctcgat
     9601 gatgaccgtg ccgccaagca tgtagccggt ctggattccg accacggtaa tcaccggaac
     9661 caacgcattc cggacggcat gacgaagcac aatggcgcgc tccgcaagtc ccttggcccg
     9721 cgcaaccgtg aggtattcct tgcccagctc ctcaaggacg gaggatcgcg tcatccgcat
     9781 cacggatgcc gccagcaagg caccaagcgc gagtgttggc aggaccagcg acttgagatt
     9841 cgcgccgata ccctccgtga ttgggacaaa ccccgtggtc ggaaagaacg accatttggt
     9901 cgagacaaac aacaccagca tggtcgcgat caagaaattg ggaacggaga gtccaaccag
     9961 cgcgaccaac cgcagcacac tgtcactcat ccggccccgc ctcagggccg aaacgatccc
    10021 cgccgggatc gccagcacga gcgaagcgat tgtcgccaaa cccgcaagct ccagcgtgac
    10081 cggaagccga ccgatgatct cgctcgcgac cggtttgcca gaccggagcg atgtccccat
    10141 atccccttgc agcacattgc caagccacaa tacgtactgg accgggagag gcttgtcgag
    10201 cccataggag cgggcgatat cctctcgctg ctgctcactg acatcgggac caactccgat
    10261 gagcacatca acaaaatcgc ccgggatctg acgcatcgcg ccaaacacca ggatggagat
    10321 accgagtagg gttggaataa agacggtgat aatccgcctg gcaagaaaac tgagcatgaa
    10381 gatgctccag tgggttcgcc catctgaaca acgatgttca tcgactacgc tgcaggggga
    10441 gcagccgaac tcctccccct gcagccggca cacggcccgt cagtggacga tgtatgacca
    10501 agggttgggt ggccggcagg aacgaccgtc acctccggct aacgtgtagt gagcctactc
    10561 gccgttaacc gtcgtctccg gcagcgtcat ccatgtctgc gaggccgacg gcgcaaatcc
    10621 ctcaaccgac tgtgtggttg cgaaattagc gacccgggtg cacaggaaga gcaggggagc
    10681 gtgctcagca accacaatct caagctcctg gtagaccggc ttccgctcct cgtgcgtggt
    10741 aagctcgcgt ccccggtcga gcaactcatc cactttcgga tcgctgaact ggaaggcgtt
    10801 cacggagccg tcggtataga gcgccgggta gagcgcacgg tccggatcgt taccggaacc
    10861 gttgtagctc acaaagctct cgaaatcacg cgcaatccac ttgtcgatga acgtgcccca
    10921 ctcgacctga tcgagctcaa cggtcacacc gatttccttg agctgatcct ggatgaccaa
    10981 cgcgatattg acgaactcct gtcggttcgc gcccacggtg atcttgaaag tcaggttgcc
    11041 ctggccagcc tcctcaagca acgccttcgc cgcatcgcga tccaccgagt aattcggcag
    11101 ttcctcgatg gggttcgccc agtccccgag tgtcggcacc agcggcccgg acacctggcc
    11161 ctggccgaag aagacgctct cgatgatcgc ctggcgatcg accgccatgc tcaatgcctg
    11221 acgcaccttc ggatcgtcga acggtgccac cgcgcagttg aacccgagta ggtaataatc
    11281 cgtggtttcc tgctcgatta gcgtaatgcc cgcgctggat cgcgcggttt cgatagagat
    11341 cgggtccgcc agcgccgtaa ggtggatttc accggtccgg atcgccgcga cccgggccga
    11401 ctcgtctggc agaatcctgt acgtcacgtt cgccagcttc ggcagcccgt ccatccagta
    11461 atccgggttc gcttcaaggg tcgtttccac gtcctcggcg cgttcgagca gggcgaacgc
    11521 acccgttccg accatcgtcg tctgaagatc aaccccatcg gccctgggaa cgatggcggc
    11581 atagttggac gatagcgatg ccaggaacgg tccatacgtc tccttgagcc gaaacgtcgt
    11641 cgtgcgctcg tccgtcgcct caacagactc gacaggagca aactggctgg tccatggaga
    11701 accgctctcc gggtccatca tccgctcgaa tgtgaagaca acatcgtctg ccgtcagggg
    11761 ctgcccgttg tggaaggtca cgccctccag caaggtaaag acataagtga gatcatccgg
    11821 cgtctcccag gactcagcca gccagggctg cggctggcca tcctcgtcga aaatgacgag
    11881 cgtctcgtag accgcggcga tcaggtcaaa gctcgaagcc gccgtcacca ttgccggatc
    11941 caatccgacg gcctcttccg acttgccgat gatcagctcg ccagctgccg aggatgcggc
    12001 cggagaggcg tcctgggcga tagcccgatt cgcagccaga tggccgccta ctgtggcaac
    12061 cgtgaaggca ccaccagcaa cctttgtaaa ttgacgtcga tttcctgacc aacgatccat
    12121 cttcttgaat ccttcccgaa gggtgatgcg aatttttcca tgttctgtct caagtgcatt
    12181 ctagcgcacc cggcttttgc gaatgcgtga cggtcttcca cgtctcatac aggctgggac
    12241 aaagaggacc gcggcatgtc gtctgcgggt cctttcaggg tgaggaccac gcacatcaca
    12301 tggcttcctc accctcgcgc cccgtcgcat ctcagccatc agcacgcaac gcatgatgcg
    12361 acgacatgga gaaaaggaga cattcacatg gttcagaaaa cagccccaaa catggtgaag
    12421 atggaagaaa gcgaactcac gcttgcgaac agcgatgagg acatccgcgg ttacaaggtc
    12481 gtcgacagcg cgggagagga catcggcgac gtcaaggatc tcctgattga cgaggatgag
    12541 gccaaggtac ggttcctcca agtcggttcc ggcggattcc ttggcatcgg tgatgagacc
    12601 tttctcatcc ctgtcgacgc aatcacccat attgatgaca acgcggttcg cgtcaatctc
    12661 acacgcgacc gggtaaggca gggaccaacc tatgacccga ccatggtcag ggatgacgag
    12721 tactggaaca ctgcttacag tcactatgga ttcttgccgt tctgggcacc cggttaccgg
    12781 tatcccacct tcccgcgcta tccacgtcac cggcgctaat cccccgggtc agcgggagtg
    12841 aacagaaatt cctcccccta acgagacaac aagcacgagc gcatgccaac gcccgaggga
    12901 ctccagggtt cctcgggcgt cgtgacgttt ccgtccggaa cgtatgtgca tagtgctggc
    12961 accggccctg cattgggaga gcgcagagat tatgcgtaac tttgtcttct gtaggagccc
    13021 tccatg
//
LOCUS       DASYWM010000068        11643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_508943, whole genome shotgun sequence.
ACCESSION   DASYWM010000068 DASYWM010000000
VERSION     DASYWM010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508943"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VGR29_02575"
     CDS             <1..832
                     /locus_tag="VGR29_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072504.1"
                     /translation="PEPAYDFGVMTSIPVEGVGEEGEGRAESVDEGEGDLFGDDELVG
                     AFGEDQEFAEDDEGYLSDDLDGDGLSEIGFDQNEDGTLTDDEVLGEDTNDDAVLGFNE
                     LWSPFPIQTVWKADASDLETDGTELVSEGPHVMLVHANAEDYDRILACGPILDLVEED
                     FVVVPLQPYSNSGYFGNALIQKDSGEYAAYLFSGISAGGQSGEDNPGAGGVSTPAVLG
                     EDGVFNQEDEGYLSEDLDGDGIFEIGMDDDGDGVLDENEVLGEDANDDGELTEDELSV
                     "
     gene            1043..1360
                     /locus_tag="VGR29_02580"
     CDS             1043..1360
                     /locus_tag="VGR29_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072505.1"
                     /translation="MAYDPNTPPARRRSAGPATWIALAIIVLIIGALIWWFVAGLDDD
                     EDVVDDAAVPEVDPVEEGEDEVVDEDEDVVPEEEEEDGAYRSHPDRLLAFTIDGVDVV
                     IAA"
     gene            1722..3131
                     /locus_tag="VGR29_02585"
     CDS             1722..3131
                     /locus_tag="VGR29_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEV2072506.1"
                     /translation="MSPQPMPNILFIMSDDHAAHAIGAYGSQINETPHIDRIASEGVR
                     LDTCLCTNSICAPSRAAILTGTHSHINGVRTLADDLDGRQETFPQLLQQAGYQTAIFG
                     KWHLGHGGHADPTGFDRWAVLPGQGLYHNPAFFVMGERVQLEGYVTDLITDYGLDWLR
                     SRDRERPFMLMLHHKAPHRPWEPDEKHAGLYEESDVPLPATFKDDYENRSNAAKNAKM
                     RIESHLYHYDLKYDVPEGLSPYKEKVWKYQRYIKDYLRCVASVDENVGRVLDYLDEES
                     IAEDTIVIYTSDQGFFLGDHGWYDKRFMYEESLRMPFVMRYPHEMPAGTVSDAVVTNV
                     DFAPLLLDYAGLETTDRMQGRSFRRIATGDDPPSDWPEAMYYRYWMHLADHHVPAHYG
                     IRSRQHKLIYYYGEALGTLGSIDEPTEPEWELFDLEADPREMRNVYSDPACAHVVSKL
                     SVWMHRLQREAGDAPVSEV"
     gene            3727..6030
                     /locus_tag="VGR29_02590"
     CDS             3727..6030
                     /locus_tag="VGR29_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV2072507.1"
                     /translation="MNRTLTDAIPQPIWTVRADGSVAYANPYWRAYTGLTEEASLGDG
                     WATAVHPDDVESIMQRWRVAAETREPYEIEYRFRRADGVYRWHLARVAPITGAEAAED
                     QWTGTAIDIDDRRHAEQELRASEARYRDVVDYADDIVYTITLEGVVLAVNQAVEHILG
                     YRPKELVGRSIRSVIVPEQVERSFGMLDRKLAGEERSTYEVEVLRKDGCRVTLEINNR
                     LVAAEGRPMVIHGIGRDVSGRRERSRQVELSAKIGVALTDRRPLSGQLDLCTRAIVTY
                     LDAAFARIWTIDDDDPSMLVLRASSGLYTHLDGAHGRVPVGAWKIGRIAAERRPHLSN
                     TVVDDPEVHDREWARREGMVGFAGYPLHIGDRVLGVMALFARQPLGESTLTVLSSIAH
                     AISMGIDRDRAEKAQEALLERERTARNWAQIAERRYRGLFEGVADAILVVDADRRYVD
                     ANAAATTLLGYEYDELMQLHVEDVVAKEPDWTRAEFEEFTATGQWQSQLHLRRKDGST
                     VPVEARATLVNLPDGTVFLSAIRDISERSRLEHLQRDFLAMVTHDLRSPLTAIKGQSQ
                     LLKRRSRDNAQVQNALESVLDQVGRMQTLIDDLADVVRLESGEIHLRIKPFDLVDLVH
                     EEIRVLEKQTDRHTFHVETPGGSVAGCWDRQRLGQVLQNLLTNAVKYSPDGGAIVVRL
                     EADEGRVRIVVSDRGVGIPPEHLPQLFQRFYRAEATGAGGLGLGLYITKMLVEAHGGI
                     VTASSSLGEGSTFVVSLPRTPDSATAK"
     gene            complement(6392..6952)
                     /locus_tag="VGR29_02595"
     CDS             complement(6392..6952)
                     /locus_tag="VGR29_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072508.1"
                     /translation="MGRLTQIVSYLVFACFFAIAGGLITGAAAQDASPAADASGITVI
                     DLAPGVTAEVLAGAPSDRAPDQTVYVARFVFQPNAEIFAHGHPGTTILGVASGSFGWT
                     LEEGTAYVIRGAAAGATGPAEEVTEPGTEVILEPGDAIYYEDDVVHTARGAGEEEAVV
                     LGTLVLTSGEPLLMPADMHMTATPEG"
     gene            complement(7013..7285)
                     /locus_tag="VGR29_02600"
     CDS             complement(7013..7285)
                     /locus_tag="VGR29_02600"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEV2072509.1"
                     /translation="MPRYIVEREFPDGLVIPISAEGAETCHAVVDQNADSGVTWVHSY
                     VSEDKSKTFCVYDGPTPEAIQRAGRSTNLPITRITKVSVLDPYFYQ"
     gene            complement(7378..10002)
                     /locus_tag="VGR29_02605"
     CDS             complement(7378..10002)
                     /locus_tag="VGR29_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2072510.1"
                     /translation="MELLERTPYLEEMQVCLQHAIGGEGRLLLVGGEAGVGKTVLVER
                     FCHLSRRVARVLVGACDPLSTPRPLAPFLDVASELGPDAEGLFAECAPPHQLFRGVLN
                     ALSNSSLPTVLVIEDAHWADEATLDALRFLARRIQPLRCMIIVTYRDDEVGSRHPLRV
                     LMGDIATGGAVRRLVLPVLSEQGVARIAKGSLVDPAELHRLTGGNPFYVTEVLAIREP
                     GVPRSVRDAVLARAFRLSDPARSVLDAAAVAGSTIPLSLLEQIIDIRTESVDECVEAG
                     ILRMHEQTLTFRHELGREAIADAVPPSQRAATHGRVLDALRSNSVTSGEWAQLAHHAE
                     AAGDHEAVITYARAAAEQASAQRAHRQSVAQYARALRCADSLPPGRHAELLGAFAYEC
                     YLTDQLALGIDALQKALEIWQREGEQAQEGASLRWLSRLYWITGQNVKAKQAAADAIA
                     VLEQIPPTCDLAMAYSNQAQLQMLARDMDRAIIWGGKAIALAEQIGDVETLIHALNNV
                     GTATLLSGNERGRPLLERSLHLALAEELEDHAARAYTNLGSSYGEIYQLGRATSYLRE
                     GITYCLERDLDSLRMYMTSWLALVSCYQGRWDDSVELATSVLNQPNVSTVSRIVALTT
                     LGRTHTRRGDRDVYPFLEEALALALPTGDLQRMGPVRAARAEAAWLAGDLQDVIVESR
                     MGFELAQECQHSWLVGEFAMWRWHAGDLDTVPENAAEPFARQMHGDWQGAAEAWTRLG
                     CPYEAARARASSASPAVVRQALSDFLQLTARPAAAITTRRLRELGVLDIPRGPRSATR
                     ANPAGLTTREVDVLTYMAEGQTNADIAAHLYLSIKTVEHHASSIYQKLGVASRREAIR
                     TARDLSLIPQDTGLSTSN"
     gene            10289..11461
                     /locus_tag="VGR29_02610"
     CDS             10289..11461
                     /locus_tag="VGR29_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2072511.1"
                     /translation="MSTVSTSNYDTLTLEHLTGVVGRKWTSYPDVIGAFIAEMDFGTA
                     PCVVEVLRKTVDNGFFGYLPDAMEEGLAKACAGWYERSTGWDVEPEQIHSLPDVLKGL
                     ELTLQHYAPKGGKVIVPTPAYMPFLFVPGYQQREIIQVPMLRDDDTWQYDLDAIDAAF
                     AAGGEVLIVCNPHNPIGHVLTREEMVAISEIVEKHGGRVFSDEIHSPLVYEGHRHLPY
                     ASVSEVTANHTITAVAASKAWNLAGLKCAQIVISNDADAEIWTNGASWTGHGASTLGV
                     IASTAAYNLGQEWLNSVITYLDGNRRRLAELVEQELPDVDYSMPEGTYLSWLDFSETA
                     VPMDIATFFRENAGVAITDGGACGEVGCKCVRFNFAMPRPLMEQAVGRMGEALRTL"
BASE COUNT         2357 a         3348 c         3626 g         2312 t
ORIGIN      
        1 ccctgagccg gcctacgact tcggtgtcat gacatcgatc cctgttgagg gtgttggcga
       61 agaaggagaa ggacgcgccg agtcggttga cgaaggcgag ggtgaccttt tcggtgacga
      121 tgagctggtt ggtgccttcg gtgaggatca ggaattcgcc gaggatgacg aaggctatct
      181 ctccgacgac ctcgacggtg acggcctttc ggaaatcggc tttgaccaaa acgaggacgg
      241 caccctgacc gatgacgagg tcctgggtga ggacaccaat gacgacgccg tgctcggctt
      301 caacgagcta tggtcgccgt tcccgatcca gacagtctgg aaggccgacg cctcagatct
      361 cgaaactgat ggcaccgagc tggtctcgga aggcccacac gtgatgctcg ttcacgcgaa
      421 cgccgaggac tacgaccgga tccttgcgtg tggccctatc ctcgatctcg ttgaggagga
      481 cttcgtggtc gttcccctcc aaccgtattc caattctggc tatttcggca acgcgctgat
      541 ccagaaggac tccggtgagt acgccgcgta cctcttctcc ggcatttcgg ctggtggtca
      601 gtccggcgag gataacccgg gcgccggtgg cgtgagcaca cctgctgttc tcggcgagga
      661 cggtgtcttc aatcaggagg atgaggggta cctctccgaa gatctcgacg gtgacggcat
      721 cttcgagatc ggcatggatg acgatggcga tggcgtcctg gacgagaacg aggtcctggg
      781 cgaggacgcc aacgatgatg gggaactgac ggaagacgag ttgagcgtct aacgatctcg
      841 aatagaaggc agggttggca tcgatggtga accgtcatca atgccaacac caaccggatg
      901 agcggtagcg agcattgctt ccgaggatca cgacgcagcg gcacccgcac cgtggactgc
      961 agcggaggaa cactcggacg agcgacacgc caagagttcg catgggcgca ccacatgcgc
     1021 cactcaggaa ggagcaggca ccatggccta tgatcccaac accccacctg cgagaagaag
     1081 atcggccggc cccgcgacgt ggatcgcgct cgcgatcatc gttctaatca ttggagcact
     1141 tatctggtgg ttcgtcgcgg gtctagacga cgacgaagat gttgtcgatg acgccgcggt
     1201 ccctgaggtc gatcccgttg aagaagggga agacgaggtc gtcgacgagg acgaggatgt
     1261 ggtccccgaa gaggaagagg aggatggcgc ctaccgctct catcccgatc gcttgctcgc
     1321 gttcaccatt gacggggtcg atgttgtgat cgccgcgtga catcgaactg gcaccgtcag
     1381 taacaaggta acgggttcca ctccgggatc caaagggaga tcgaaggggc ttgccatcgc
     1441 caagcccctt cgacgttgct ctgttcccta gcctctctgc ttcggtaggc atggaggcct
     1501 ggcgttgggt gacatgtcag cacttcgact atcgcagact tcctacctgt gggacatggc
     1561 aaaggtgtgt tcggttgctt caatgagtga ctgaccagat ttcttgctgg accaattgct
     1621 ggcgtctggg ctggccgggt gattcgtcca taagcaggtg acaagggacg acttgccggt
     1681 gacaatggcg gcgcaggata tccacgtcac tgggggagtt tatgagccca caacccatgc
     1741 caaacattct cttcatcatg agcgacgatc atgcggcgca tgcgatcggc gcgtacggca
     1801 gccagatcaa tgaaacgcct cacatcgacc gcattgccag cgaaggtgtg cggctcgata
     1861 cgtgcctttg cacgaactca atttgcgcac ccagccgggc cgcgatcctg accggcacgc
     1921 atagtcacat caatggggtg cggacactgg cggacgacct ggatggccgc caggagacct
     1981 tcccccagct tctgcagcag gcgggatacc agacggcgat cttcggcaag tggcatcttg
     2041 gtcacggagg gcatgccgac ccgacggggt ttgatcgctg ggcggtcctg ccgggccagg
     2101 gactttatca caaccccgct ttcttcgtga tgggagagcg ggtgcagctt gaggggtatg
     2161 tgacggatct catcacggac tacgggctgg attggctccg tagccgggac cgcgaacggc
     2221 ccttcatgtt aatgctccat cacaaggcgc cgcacaggcc atgggagccg gacgagaagc
     2281 atgctggtct ctatgaagag agtgatgttc cactgccggc gaccttcaag gatgattacg
     2341 agaaccggtc gaatgccgca aagaatgcga agatgcggat cgaatcccat ctctaccact
     2401 acgacctcaa atacgatgtt cccgagggtc tctcgcccta taaggagaag gtctggaaat
     2461 accagcgcta tatcaaggat tacctgcgct gtgtcgcctc ggtcgatgag aatgtcggtc
     2521 gcgtgctgga ctatctggat gaggaaagca ttgccgagga tacgatcgtc atctatacct
     2581 ccgaccaggg atttttcctg ggggaccacg gctggtacga caagcgcttt atgtacgagg
     2641 aatcgttacg gatgcctttc gtgatgcgct atccgcacga gatgccagcg ggaaccgtct
     2701 ccgatgccgt tgtcaccaat gtcgacttcg cgccgctcct cctagactac gccgggttgg
     2761 agacgaccga ccggatgcag gggcggagtt tccggcgcat agcgacagga gatgaccccc
     2821 cttcggactg gccggaggcg atgtactatc gctattggat gcacctggcg gatcatcacg
     2881 tgcctgccca ctacggcatc cggagccgcc agcacaagct gatttactac tacggcgagg
     2941 cgctcggaac cttggggtcg atcgatgagc cgaccgagcc ggagtgggag ttgttcgacc
     3001 tggaagcaga cccacgggag atgcggaacg tctatagcga ccccgcctgt gcccatgtcg
     3061 tctcaaagtt gtcagtctgg atgcatcgat tgcagagaga ggcaggagac gctccggtga
     3121 gcgaagtcta gagcgactcc ggccgctggc cttgtatctg gcctctggcc gcccgcacct
     3181 gtagcaccag acgtacctca aacggcataa caccctgtga ttctggagac catggtctcc
     3241 agtgctcggc gcgcacattc atgaacaatc ccattggctc cacgtgagga aggaatcgtt
     3301 cgcgaggagg ccgcccgggg acctcccaag gctgctcctg tagattaggg ccgccgctcc
     3361 gtcgggagga tgccgtcgga tgaatctctc atatgtgcat gtaccagcgg cttgaattgc
     3421 cgggcatcat aagctcacgg tgttcatcca gtgttaccgg cagacgtgga accacagtcc
     3481 catagagttc ctggatatcg gattcaagct gattttcgac ttcggcatct tccacgtcag
     3541 ggcctcgttg ctggcatcgg tccgttccat gtcgttgcac gcgtgagatg cctccccggt
     3601 ctggcggcca tgggaccgcc aatgccgggt aacatgtcgg cactccgatt atgattgttg
     3661 gaggatgtgt cagatccaat gagcgccagt gcatgtacgc ggtcagcagg gattcgggag
     3721 agcggaatga accggacgtt gaccgatgcg atcccgcagc caatctggac cgtccgggca
     3781 gacgggagcg tcgcgtacgc caatccttac tggcgcgcct acacgggcct cactgaggaa
     3841 gcctcacttg gagacggatg ggccaccgcc gttcatccag acgatgtaga gagcatcatg
     3901 cagcggtggc gagtggcagc cgagacccgg gagccctacg agatcgagta ccggtttcgt
     3961 cgcgcggacg gcgtgtatcg ctggcatctc gcccgtgtcg ccccgatcac tggagccgag
     4021 gcggctgagg atcagtggac cgggacggcg atcgatatcg acgaccgtcg tcatgccgag
     4081 caggaattgc gggcaagtga agcccgctac cgcgatgtcg tcgattacgc cgatgatatt
     4141 gtctacacca tcacgctcga gggagtcgtc ctggccgtga accaggccgt cgagcacatt
     4201 cttggatatc ggccaaaaga gcttgtgggc cgctcgatca gatccgtcat cgtgcctgaa
     4261 caggtcgaac gatcatttgg catgctcgac cgcaagctgg caggggagga gcgtagtacc
     4321 tacgaggtcg aggtgctgcg caaggatggg tgccgggtca cacttgagat caacaatcgc
     4381 ctcgtcgcgg ccgaaggccg gccgatggtc attcacggca ttggccgcga cgtcagcggg
     4441 cggcgggagc gctcccggca ggtcgagctg agtgcgaaga tcggtgtcgc ccttaccgat
     4501 cgccggcccc tctccgggca actggatttg tgcacgcggg cgatcgtcac atatctcgac
     4561 gcagccttcg cccggatctg gaccatcgac gacgacgatc cctcgatgtt ggtgctccgt
     4621 gccagttccg gtctctatac gcatctggac ggtgcacacg gtcgggtccc cgtcggtgcc
     4681 tggaagatcg gacgaatcgc tgccgaacgc cggccccacc tttccaacac ggtcgttgac
     4741 gaccccgagg tgcatgaccg ggaatgggca cggcgcgagg ggatggtggg ctttgctggc
     4801 tatcccctgc acatcgggga tcgggtgctg ggcgtgatgg cgctcttcgc tcgccaacca
     4861 ctcggtgagt ccaccctgac cgtgctgagc tctatcgcgc atgcgatctc gatggggatc
     4921 gatcgggacc gtgccgagaa ggcgcaggag gcgctgctgg agcgggagag aaccgccagg
     4981 aactgggcac agatagccga aaggcgctac cggggactct tcgagggggt ggccgatgcg
     5041 atactcgtcg tcgatgccga ccggcgctat gtggatgcga atgcagccgc gacaaccctg
     5101 ctgggctacg agtatgacga gctcatgcag ctgcatgtcg aagatgtcgt cgccaaggaa
     5161 cccgactgga cgcgtgcgga attcgaggag tttaccgcga caggacaatg gcaatcccag
     5221 ttgcatctcc ggcgcaagga cggctccacc gtacccgtgg aagcacgggc cacgctggtc
     5281 aatctgcctg acggcacggt ctttctgtca gcaatccgcg acatcagcga gcggagccgg
     5341 cttgagcact tgcagcgcga tttcctcgcg atggtgacgc acgatcttcg cagtcccctg
     5401 acggcgatca agggtcagtc acaattgctg aagcgacgtt cacgcgacaa cgcgcaagtg
     5461 cagaacgcgc tggagagcgt cctcgatcag gtcggacgta tgcagacgct catcgatgat
     5521 ctcgccgatg tcgtgcgact cgaatcaggc gaaattcact tgcggatcaa accgtttgac
     5581 ctggtggacc tcgtgcatga ggagatccgc gtgctggaga agcagacgga ccggcacacc
     5641 tttcatgtgg agactcctgg tggctcggta gctggttgct gggaccgtca gcgactgggt
     5701 caggttctcc agaatctcct tacaaatgca gtcaagtaca gcccggatgg tggggcgatc
     5761 gtggtgcggc tggaggcaga cgaaggcagg gtccggattg tcgtaagcga ccgtggcgta
     5821 ggcataccac cggaacacct gccccagctc ttccagcggt tctaccgggc tgaggcaacg
     5881 ggggctggtg gactggggct tgggctctac atcaccaaga tgctcgtcga ggcacatggc
     5941 ggcattgtca ctgcctcatc gtcgctaggg gagggcagca cgtttgtcgt gtcgctgccc
     6001 cggacgccag attccgctac ggcgaagtag ccgcagggac tgtatctcga tctgctcctg
     6061 ttgcgacccc gcccgtccga gtaactacgg agatcgccat tggcatcccg atcacagaat
     6121 ggttcaggtt tgggtggcct cccacagggc aatgacgcgg ttcctcgtga atctggcgcg
     6181 ctctctgcca cccctcggag tctacggtca catccatacg tgcacataga cctcctgcca
     6241 gcggtcttag gcaggaggtc tatgtgtgga ctatggggaa gcgattcagg agggaatcct
     6301 cgtcgccagc gactgccgtg tagcgttgcg ggaaaagcaa caatgacggg gttcagatat
     6361 ctgcggcctg tggcagggtc gagctactct cctatccctc cggtgtcgct gtcatgtgca
     6421 tatcagccgg catcaggagg ggttcgccac tggtcagcac cagcgttcca agcaccacgg
     6481 cctcttcctc accagctccg cgggcggtgt ggacgacatc gtcctcgtag tagatggcat
     6541 cacctggttc gagaatgact tccgtccccg gttctgtaac ttcctccgcc ggccctgtcg
     6601 ctcccgccgc ggctccacgt atgacatatg ctgttccttc ttccaaggtc catccgaagg
     6661 agccagaagc cacgcccaga atggtcgtgc cagggtggcc gtgcgcaaag atttccgcat
     6721 taggttggaa aacaaagcgg gccacgtaaa cggtctggtc cggtgcccgg tcggagggcg
     6781 cgcccgcgag cacctcagcg gtcacgccgg gagccaggtc aataacggtt attcccgagg
     6841 catccgcggc cggcgaggca tcctgagcgg ccgcgccggt gatgaggccg ccagcaatgg
     6901 cgaagaaaca ggcgaagacg agatacgaaa cgatttgagt caagcgaccc atgtgggatt
     6961 cctttctcat gaccagctag cagtagaggg gcgatgtctg ccgcctggtc catcattgat
     7021 agaaataggg gtcgagaacc gataccttag tgatccgggt gatcggcagg ttggtagacc
     7081 ttcccgctcg ttggatcgct tcaggggttg gaccgtcata gacgcagaac gtcttgctct
     7141 tgtcttcaga gacgtaggaa tgaacccagg tcacgccgct gtcggcgttc tggtcgacca
     7201 cggcgtggca cgtttccgcc ccctcagcag agatgggaat caccagaccg tctggaaact
     7261 cccgttcaac aatgtatcgc ggcatgctgc tacctcctcc tgaatcgaaa cgcaccggaa
     7321 catccggctt ccttgatgat gccggaccca ggcgaatgtc gaatcggggg aataccctta
     7381 gtttgaggtt gagagccccg tatcttgggg aatgagggag aggtcgcggg ctgtgcggat
     7441 agcctcgcgc cgtgaggcga cgcccagctt ctgatagatc gaggacgcgt gatgctctac
     7501 cgtcttgatt gagaggtaga gatgcgcagc aatatctgcg tttgtttgcc cttctgccat
     7561 gtaggtaagg acgtcaacct cgcgtgtagt caggccagct ggattggcac gggtagccga
     7621 gcgaggaccg cgcgggatgt ccagcacgcc caactcgcgc agccggcgtg tggtgatcgc
     7681 agcggctggc cgcgcggtga gctgaagaaa gtcagacaat gcctgccgta ccactgccgg
     7741 gctcgcactg gacgcacggg ctcgggcagc ttcgtatggg cagcccagac gggtccacgc
     7801 ctcagccgct ccctgccaat ccccatgcat ttgtcgcgca aacggctcgg cggcgttttc
     7861 cggtaccgta tccaggtccc cggcatgcca cctccacatt gcaaactcgc ccacgagcca
     7921 ggaatgctgg cattcctgag caagctcgaa acccatgcgg ctttccacta tgacgtcctg
     7981 cagatcgcct gccagccatg cggcttcggc ccgggcagct cgcaccgggc ccatgcgttg
     8041 gagatcacct gttggcagcg ccagtgccaa ggcctcttca aggaacgggt acacatctcg
     8101 atcaccgcga cgcgtgtgcg ttcgaccaag agtggtgaga gccacgatcc gactgacggt
     8161 cgatacattg ggctggttga gtaccgacgt agcgagctca acagaatcgt cccagcgtcc
     8221 ttgatagcag gagaccagcg caagccatga cgtcatatac attcgcagag agtcgagatc
     8281 gcgctccagg cagtaggtga ttccttcgcg aagataggac gtggcccggc caagttggta
     8341 gatctcgcca tagctggacc caaggttggt atatgccctg gcggcgtggt cctcaagttc
     8401 ctcagccagc gcgagatgca gactgcgttc gagaagagga cgaccccgtt cgttaccgga
     8461 gagaagtgtc gcggttccga cgttgttgag cgcatgaatc agcgtctcta cgtcaccgat
     8521 ttgttctgca agggctatcg cctttccgcc ccagatgatc gcccggtcca tatcgcgtgc
     8581 aagcatttgc agctgggcct gattgctgta tgccatcgcc aagtcacacg tgggaggtat
     8641 ttgctcaaga actgctatgg catctgcagc tgcctgtttt gccttaacgt tctgtcccgt
     8701 tatccagtaa agacgagaga gccatctcag acttgctcct tcctgggcct gttctccttc
     8761 acgttgccag atttccagcg ccttctggag cgcgtcaatg cccaaggcaa gctggtcagt
     8821 gagataacat tcgtacgcga aggctcccaa caactctgcg tgacgcccag gggggagcga
     8881 atctgcgcac cgcaatgccc gtgcatattg agcaacggat tgtcggtgtg ctcgttgagc
     8941 agacgcttgt tcggcggcgg cacgagcata cgtaatgacg gcttcatgat ccccagctgc
     9001 ttccgcatga tgcgccagtt gtgcccattc ccctgaggtg acggagtttg agcggagagc
     9061 atccagtaca cgtccgtgtg tcgcggcacg ctgactgggt ggaaccgcat cggcaatggc
     9121 ctccctgcca agttcatggc ggaaggtcaa cgtctgctcg tgcatgcgga ggatgcccgc
     9181 ctccacacat tcgtcaacag attcagttcg aatgtcaatg atctgttcaa gcaagctcaa
     9241 cggtattgtg ctgcccgcta ctgctgctgc atcgagtacc gaccgggcgg gatcagaaag
     9301 ccggaaggcg cgggccagca ccgcatctcg cacggagcgg ggtacgccgg gttcgcgaat
     9361 tgcgagcacc tcggtgacat agaacgggtt tccaccagtg aggcgatgta gctctgccgg
     9421 gtcaacgaga ctgcctttgg caatccgggc gacgccttgc tcggagagca cgggcagtac
     9481 caatctccgg accgcccctc cggttgcaat atcccccatg aggacgcgca acggatgcct
     9541 ggagcccact tcgtcatccc gataggtgac gatgatcata cagcgaagcg gctggattcg
     9601 ccgtgcaagg aaacgaagtg catcaagcgt cgcttcatct gcccagtggg cgtcctcgat
     9661 caccaggacc gtgggcaggg acgaattcga cagcgcattc agcacaccgc gaaacagttg
     9721 atgaggcggg gcgcactctg caaacagacc ttcggcgtcg ggtccaagtt ctgacgctac
     9781 atcgagaaaa ggagcgaggg gacggggcgt cgagagggga tcgcacgcac caacaaggac
     9841 gcgcgctact cgacgtgaga ggtggcaaaa ccgctcgaca agaacggttt tgccgacacc
     9901 tgcctcgcct cccaccagta gcaagcgccc ttcaccacca atcgcatgct ggaggcaaac
     9961 ctgcatttct tcgaggtagg gagttcgttc gaggagctcc atgagctgcg tctccacaag
    10021 tttgagttac agtgctgtgc atttatggta gatcaattat cggtttcttg tcatatgcga
    10081 caccaccatg ttcctcgcga gggggtctga cccaacgagg tttgacttga gcacggcatg
    10141 aggtgcgcac tgacatcgca cggcctttgc agcattcatg agaccgaagt agtggatact
    10201 gtgtggaatt caggcccgta tcagggcggt cccgtacaga acagttcttt ccaacgattc
    10261 caatgaggca aggagacaac ccgaggacat gagcacggtg agcacatcca actacgacac
    10321 cttgactctg gagcatctga cgggtgttgt tggcaggaaa tggacttcct atcccgacgt
    10381 gatcggtgcc ttcattgccg agatggattt cggcactgct ccgtgtgtcg tcgaggtgct
    10441 gcgcaagacg gtcgacaatg gctttttcgg ctacctcccg gacgcaatgg aggaggggct
    10501 agccaaggct tgcgcaggct ggtacgagcg cagcacgggt tgggatgtcg agccggagca
    10561 gattcattcg ctgccggacg tgctgaaggg tttggagttg accctgcagc actatgcgcc
    10621 caagggtggc aaggtgattg tgccgactcc agcctatatg cccttccttt tcgttcccgg
    10681 ttaccagcaa cgcgagatca tccaggtgcc gatgctccgc gatgacgata cctggcagta
    10741 cgatctcgac gccatcgacg ctgcattcgc cgccggcggg gaggtgctga tcgtctgtaa
    10801 tccccacaat cccatcggac atgtccttac ccgggaggag atggtcgcga tcagcgagat
    10861 cgttgagaaa catggcggac gagtcttctc ggacgagatc cattcaccgt tggtttacga
    10921 aggacaccgg catcttccgt atgcgtcggt ttccgaggtg accgccaacc acacaatcac
    10981 cgcggttgcc gcgtcgaagg cgtggaatct cgcgggcctc aagtgcgcgc agatcgtgat
    11041 ctccaatgat gccgatgccg agatctggac gaatggggca tcctggaccg ggcacggcgc
    11101 ctcgaccctg ggcgtgatcg cgagcaccgc cgcctacaac ctaggccagg agtggctgaa
    11161 tagcgtcatc acgtatctgg acggaaaccg ccgtcggctg gctgagttgg tggagcagga
    11221 acttccggac gtggattaca gcatgccgga aggcacctac ctgagttggc tcgacttcag
    11281 cgagacggca gtgccgatgg atattgctac cttcttccgg gagaacgcgg gtgttgcgat
    11341 caccgatggc ggcgcgtgtg gtgaggtcgg ctgcaagtgt gtgcgcttca acttcgccat
    11401 gccacgcccg ttgatggaac aggctgtggg gcgaatgggc gaggcactga ggacgttgta
    11461 gaagttcttc gggggatcac cgacgataca tctttcggta acacatcttg ctggttcatc
    11521 caaaggtgtt tgcccattcc catcttgcaa gatcatggcg gcacagagtt ggcctgagca
    11581 gaggggctcc ttgtatggca acccttggat gtcgattgac gatgcaggac cacggttcat
    11641 gac
//
LOCUS       DASYWM010000069        41578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_340260, whole genome shotgun sequence.
ACCESSION   DASYWM010000069 DASYWM010000000
VERSION     DASYWM010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 41578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41578
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340260"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VGR29_02615"
     CDS             <1..575
                     /locus_tag="VGR29_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEV2072512.1"
                     /translation="FDPEPTFFELTGQTVLLLGTGAIGKATAERLRPFGCTIIGARRT
                     PQVLNGFDWVVGFGELGDILPNVDHVVSSLPMTPGTAKLFSSEMIGRMKRGAYFFNVG
                     RGGTVDQEALIEALVNGHLGGAGLDVTDPEPLPVDSPLWSMPNVLITSHTSGGSPKAD
                     ERVAELAMENIRRYCAGEDLLNLVDLEHGY"
     gene            complement(630..1829)
                     /locus_tag="VGR29_02620"
     CDS             complement(630..1829)
                     /locus_tag="VGR29_02620"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HEV2072513.1"
                     /translation="MSHDSLKRRAVRAGVAAFGCDWLVDGWAIAPGRIELIGNHIDYN
                     GGPVLAAAIDRVVVVGSGNLNAPRDICMVAADVNGQRHTFDALTLGDWRAGEGDSGPV
                     VYLKGVVAALIARGIPFRAGVGLAISGDVPRGFGMSSSAALCIATLLALTVDEIEPLD
                     MVAIAREAEHRAGSPVGAMDQSASVAGEVIRFDGRDNSFSRLSPSLGDLVFAVASSGV
                     GRSLRTSSYGARVRETEEARQILADAYDLDLPHLSALTPHWDNVMPTIHSHLNQTLLK
                     RVRHVVSETRRVGKAETAMRAGDWSRFGRLMNASGESSARDYEISHSRVEALVHLLRE
                     QEGVLGARMMGGGEGGPALALLHKDAVSSVSNRLEREFYNSRKMATDTTVLQRCVFGP
                     GAHREDA"
     gene            complement(1840..2547)
                     /locus_tag="VGR29_02625"
     CDS             complement(1840..2547)
                     /locus_tag="VGR29_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEV2072514.1"
                     /translation="MTDGQDRSDYQTYMWVVAHPDDAEFSSSGTITKLSREGKRIVLI
                     QVTSGDRGTADRSQTPETLGATREKEQLEAARREGIDEVVFLRQGDGHIVPDIVLREK
                     IVKQIRIHKPDVVITHDPFRPYAMHPDHRGVGQATHDSVYPTARDHLYFPDHLAEGIE
                     PHKTAEIWYFGSEAPDVFVDITDTFENKIEALKAHVSQVGDGATLDERLRERSRELAK
                     DQPFELAEAFKSVKMNR"
     gene            complement(2600..3916)
                     /gene="hisS"
                     /locus_tag="VGR29_02630"
     CDS             complement(2600..3916)
                     /gene="hisS"
                     /locus_tag="VGR29_02630"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV2072515.1"
                     /translation="MAPRKPQVLKGFRDYLPEQMLLRQQVMGSIREVFEQHGFEPLDT
                     PALEYMEILTGKAGENEKLMYHFTDQGEREVGLRYDLTVPLARVVAMHQNDLVLPFKR
                     YHIAPVWRAEKPQRGRFREFWQCDADIVGPGSALVDAEAISVLTDALRAIGLGHAVVV
                     INHRKLLEALASMAGVSPHQAPTIYRAIDKLDKVGRDGVLSELMRGAVSEEAANQVLD
                     VVTTSGTNEELLDLAHEYLHDYPDGVQAVRELRELIDHIALLGVHKNAYRIDLALARG
                     IDYYTGPVYEAVVEELQVGSVAGGGRYDGLVGTFLGRDIPATGISLGIERILEVIQEF
                     DLVPRRQSVADVFLVHREETLADASALARDLRETGIHVDQSVLTKKGMGDQLKYADRR
                     GIPFAVIQGPDELARDEVAIKNLRTGEQSMVARPMVVQELSHLLKA"
     gene            complement(3950..4495)
                     /locus_tag="VGR29_02635"
     CDS             complement(3950..4495)
                     /locus_tag="VGR29_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEV2072516.1"
                     /translation="MRSPFEPSGNMANSASTDEHRADAPKSVACAVLTISDTRTREDD
                     RSGQLIRQHLDWRGHTITAYEIVPDDGSRITEVLERWIADDNIQAIVTNGGTGIAGRD
                     VTYDAITGVLEKRLDGFGEIFRMLSFAEIGAAAMLSRAVAGVSNNTAIFATPGSSNAV
                     RLAMEKLIGPELAHVVHEIAK"
     gene            complement(4598..4978)
                     /locus_tag="VGR29_02640"
     CDS             complement(4598..4978)
                     /locus_tag="VGR29_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEV2072517.1"
                     /translation="MPARRNKDAIGIAGERHARMMLEAHGYQFLASNWHCSAGELDLV
                     MLDRDLLVFVEVKTRRGERAGRADDAVSPAKARRMLATAEWYFGEHPEHQDRTWRCDL
                     VAITIDPKMGAAQANHYENAIVTG"
     gene            complement(4959..5627)
                     /locus_tag="VGR29_02645"
     CDS             complement(4959..5627)
                     /locus_tag="VGR29_02645"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV2072518.1"
                     /translation="MLDIAPELSTLTYELALHSEGILAIAGVDEVGRGALAGPLVAAA
                     VILPTPESLRQDVDFWRTVRDSKTIPEAKRAAIAEGIKSRVPSWSTADVEPDELDELG
                     LGPSNRIVMERAVLGLDIEPEVLLIDAMTIESSTLQIGIIDGDALSLSIAAASIVAKV
                     TRDALMVDASRTWPVYGFARHKGYGVRSHIAALRQHGPCVLHRRCFAPVRYAQELVDA
                     RQAK"
     gene            complement(5628..6569)
                     /locus_tag="VGR29_02650"
     CDS             complement(5628..6569)
                     /locus_tag="VGR29_02650"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV2072519.1"
                     /translation="MIPPAISNTHVLTLLRERLGLDISGVETLRPGAWSSVFAYAEGH
                     RRWVIRFSNHGDDFDRDAYAFRFWSPDLPVPEVTHRGSLDGISYAVSQRVPGDVLDKL
                     DRADYERTLPSLLGTLNALRIADVTSSTGYGGWDSSGNGMATSWPGHLRASLEDSPDQ
                     RGGGWRPRLEQSPTGAHAFDRDVIVLERMLKDMPNVRHVIHSDLLNYNAFVSDHHLSG
                     VIDWGCAMYGDFLYELAWFEFWSPWYPKWQGISVATEAHRFFADLGVDLHNFHERLLC
                     YQLHIGLSHQAYNASIGRWDDLADVTRHTVRVADRVR"
     gene            complement(6934..7281)
                     /gene="rplS"
                     /locus_tag="VGR29_02655"
                     /pseudo
     CDS             complement(6934..7281)
                     /gene="rplS"
                     /locus_tag="VGR29_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(7442..9322)
                     /locus_tag="VGR29_02660"
     CDS             complement(7442..9322)
                     /locus_tag="VGR29_02660"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEV2072520.1"
                     /translation="MAHPLLERLDAGVLLGDGAMGTMLYEAGHELDTCLDALNLTHPA
                     TIALVHRSYLNAGADIIETNTFGANRFRLELHGLADKVRDINFRGVKIAREEREISGR
                     PVLVAGSIGPTGRTLEPYGTLPKEEAQEAFAEQIGALLEAGVDLLMIETMGDLNEMDA
                     AIAAAREASDLPIIALMTFAEDGLTLSGASPEQVIRHLAEQRVDVVGANCSVGPQRLL
                     GVIRRMHEELSNTATAEDTPRIAAVPNAGWPSRVGSRLIYQSSPEYFAQFAQEAMIAG
                     ARLIGGCCGTTAAHTNAMRHAIDSGEADHRPSPNGRASQNSGPQVAVITIPESDEDDT
                     EVKKALGKKFLKCVEIDPPKGLNPQKALDGARMLKEAGVDAINVADSPMARVRMSAMM
                     LCYLIQHQIGIETIIHFTTRDRSLMGLQSELLGAHAAGVRNILALTGDPPSLGDYPDS
                     SAVYDVDSIGLIRVIRRMKQGEDSSGSSIGRAANFTIACAVDPTKPDLDEEVRRLREK
                     IDAGADFVMTQPIFDMRVWTNFLELYGVDRLPVPVMIGILPLQSSRHAEFLHNEVPGI
                     TLTDDARERMRKAGNSGRKEGVKMAQELLHELRPHAEGVYLMPSFGRYEVAAEVIQVL
                     ED"
     gene            9626..10471
                     /locus_tag="VGR29_02665"
     CDS             9626..10471
                     /locus_tag="VGR29_02665"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEV2072521.1"
                     /translation="MSSETLSASSEEQSGSVSNRRGILMPIGGAEDKLDNKVILSEFV
                     EHAGGEKARIAIVPTASSIEAAGLRYKAIFLGMGVESAEVVYIGSREDANGGESLDAI
                     ADATGIFLTGGNQMRLSAIIGGTEFEQLVRERHNAGVIVAGTSAGASILSAHMVALGA
                     NGATPKLRMAQMFAGFGLISNVIIDQHFRQRDRIGRLLSLVAGNPGLLGLGIDEDTAL
                     VIDDSGIMKVVGRHSVTIVDGSHMVSDIFQVKRYGEITISDVRLHVLGPGRKFDMTSR
                     KMIRE"
     gene            10613..13246
                     /gene="cphA"
                     /locus_tag="VGR29_02670"
     CDS             10613..13246
                     /gene="cphA"
                     /locus_tag="VGR29_02670"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEV2072522.1"
                     /translation="MLNIRRTTVYRGPNIWARMPVIHYVLDIGELEDRPTNKIPGFYE
                     HLTELIPSLYDHGCSLGRPGGFLQRLREGTWMGHVLEHVALELQNLAGAEVARGKTRQ
                     AGERGVYNVIFQYEQEDVGLAAGALALRLLNHLIYGSEPDFDFVHEHEEKVIKLAERF
                     AYGPSTGAIVHEAHRRGIPVLRLDSRRSLVQLGHGKYQRRIWATVTSETANIAVQVAS
                     NKELTNRLLHEIGIPVPRSQIVQSADQAVSAAARLGYPVVIKPLDGNHGRGVFVNLSD
                     EGEVREFYDSAQAESRSGQIIVETFIPGKDYRILVVNNEVVAAAERVPAHVIGDGEHT
                     VQELIALANQDPRRGVGHEKILTRITVDSQTMEVLERDGRTLETVAQDGEFVQLKLTG
                     NMSTGGTSIDRTDDIHIDNIEIARQAAMVVGLDIAGIDLITQDISRSVREYGGGICEV
                     NAGPGFRMHTHPTEGHPRHVGRAVVDMMFPQGEPTRVPIVAVTGTNGKTTTSRMIAHI
                     MKTSGKTVGLTTTDGINIDGTQIMAGDMSGPTSARMVLKNPTIDYAVLETARGGILRS
                     GLGYDRCNIGVVTNVSGDHLGLKGIDTLAGLARVKEVVPASVLRDGASVLNADNEWTV
                     QMTRSARGEIIFFSMDEENPVIRDHIRQRGRAVLLKQTRQGEMITIYESRRETSLLLA
                     SQIPATYEGRVRVNIENAMAAAAAALAADVQLDYIRLALRTFSSSFYQTPGRFNLLDY
                     GNKRIVMDYCHNVAGLESIADFVKRMEADATLGVISIPGDRSNDDMRAFGELAGKTFD
                     RIIIREDNNTRGRARGEIAGLLKEAVLSTGKPSDEVDVILDELEAAKQGVERANRGDL
                     VVLLVDKPVAVWEMLTGDRPQ"
     gene            complement(13279..14532)
                     /locus_tag="VGR29_02675"
     CDS             complement(13279..14532)
                     /locus_tag="VGR29_02675"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2072523.1"
                     /translation="MSDRRAHAFSTLRFRDFRLLWFADSVSILGTQIQRIAITWHVFE
                     LTGDPFQLGLLGLFRFVPVLVFGLLGGVIADHRDRRMVLIFTHLALLASTGTLAAATL
                     AGEVSVGLIYSITFISSTFNAMAGPARQALIPNLVPRSSLAGAATMANLSMQAAMVAG
                     PAIGGVIIAQTSLALAYMLDAASFIAVIAAALLIRTRQARVPITERGLSAIKAGFVLL
                     WHTPILLAVMSLDFIATFFGAITNLMPIFAGEILGGGANSLGLLLSAPAAGAILGSLV
                     FGLFTMPQKPGFGMVACVIAYGACIVGFGLTNNIWLALLFLAGSGAADSISMALRHAI
                     RNLITPDAFRGRIAAAHSAFAMGGPQLGEFRAGAMASWMGAPAAVAIGGVGTILGTIA
                     MVRLVPTILRYRSTEEAPVAEAASA"
     gene            14637..15164
                     /locus_tag="VGR29_02680"
     CDS             14637..15164
                     /locus_tag="VGR29_02680"
                     /inference="COORDINATES: protein motif:HMM:NF002598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HEV2072524.1"
                     /translation="MSTSGGVLSREQLKVLIEDPDRPLLREYVDLDGQLQPNGFDVTL
                     REVSRHSDGGAIGVTSEDRVLPDLERLPFDEGGWLTLAPGSYHILYNEVVDIPDSLMA
                     LGRPRSSLNRCGVTIHTAVWDAGYNGRSTSLLVVANSGGFRVQRNARVMQLVFFGMSE
                     APADGYRGSYQLENM"
     gene            15573..16028
                     /locus_tag="VGR29_02685"
     CDS             15573..16028
                     /locus_tag="VGR29_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072525.1"
                     /translation="MPEKGQTLPASVYEELLALLLERFGEGWASDATHHRQFGTSMTS
                     VIQTSEGMHLIGSPEIEKNSVNFNLWVEHPVEDVWEADELAFAVFAQVAEDIFLSSRQ
                     IEERGVRYRFVTGSIESGYIGSLHFTGPHAKELADIYRMRITQGHHYHA"
     gene            16021..16398
                     /locus_tag="VGR29_02690"
     CDS             16021..16398
                     /locus_tag="VGR29_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV2072526.1"
                     /translation="MPEAKMWTVDVDGIGSVEVAEGRRLVLGLEDGGIDILHRCGGNA
                     RCATCRVEVLEGQPTDITDAEEGRLDRLTNRQDTTRLSCQIRVTDNLSVQVCNRLSEQ
                     PQLGDAGPRPIEWPADSALPPEE"
     gene            16456..16717
                     /gene="ffs"
                     /locus_tag="VGR29_02695"
     ncRNA           16456..16717
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGR29_02695"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(16802..17206)
                     /locus_tag="VGR29_02700"
     CDS             complement(16802..17206)
                     /locus_tag="VGR29_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072527.1"
                     /translation="MWNDLTSVPDSGEVLNIPSLAAASVFALVFIIAAFYSARPRAKP
                     LGERYSRRFIQRASTLLGWISGFGLFLIIIRYLQINPLSLGLPIWTLLTVLGLLLAII
                     MVAIRAPSDEEARRRNRATHRRTRRPARRARR"
     gene            complement(17223..17936)
                     /locus_tag="VGR29_02705"
     CDS             complement(17223..17936)
                     /locus_tag="VGR29_02705"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEV2072528.1"
                     /translation="MEVTLRIKRFNPETDTKAHFEEYKVEVEGTDRVLDALNYIRWYH
                     DGTLAYRRSCAHGICGSDAMRINGRNRLACKVLMQDLGKRVTLEPLIGFKVEKDLLVD
                     MDQFFDSYKSVKPYLIAEDSPTARERLQSPAERHRFDDTTKCILCAACTTACPITWTN
                     DEFVGPAAIVNAHRFIFDSRDQGTAERLQILNKGSGVWRCRTVFNCTDACPRDIKVTE
                     AIEQVKRALITGEVRYTDA"
     gene            complement(17992..19725)
                     /gene="sdhA"
                     /locus_tag="VGR29_02710"
     CDS             complement(17992..19725)
                     /gene="sdhA"
                     /locus_tag="VGR29_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEV2072529.1"
                     /translation="MAYHRYDAVIVGAGGAGLMAAIQLAGKANIAVVSKLYPTRSHTG
                     AAQGGISAALGNTEEDHWEWHMYDTVKGGDYLVDQDAAEVLTREAIEAVLELEHMGLP
                     FNRTPDGRIDQRRFGGHSRNFGEGPVRRACYAADRTGHMIIQTLYQQGIKHNVNFFDE
                     HHLVDVIFAADGTCCGVVTYQIKTGELHTIHAKTVLIASGGFGRVFKVTSNAMAGTGD
                     GVAIPFRHGIPLMDMEMYQFHPTGLYKLGILLSEAARGEGGVVRNSEGEAFAARYAPT
                     MKDLAPRDMMSRFIHQEIREGRGIDGKDYVLLDVSHLPPEVIDEKLPDITDFARVYLG
                     VEPKEEGVPIQPTAHYAMGGLPTNIEGQVIRDPAGTAVPGLYSAGESACVSVHGANRL
                     GTNSLVDLVVFGRRAGKRMYEEIQNVNWAELPPNPEDNARSDIDTLLNRQSGERIADI
                     RSEMQEVMMDKVSVVRNEIGMQEAQARISELKSAYANAAIMDRGRIYNMDLLEALELG
                     NMLDCAETIIEGALARKESRGAHYRDDYEARDDQNWLAHSMVYRNDDGSLRSEKKPVV
                     ITTMQPKERKY"
     gene            19998..21155
                     /locus_tag="VGR29_02715"
     CDS             19998..21155
                     /locus_tag="VGR29_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2072530.1"
                     /translation="MPSTQSPPLPDVLNVAETYVEPIVELTIRLSEVPAPTNDEALRA
                     AAVREELEQLGYEDVTEDELHNVTGRIAGMDSSLCLLLAGHIDTVFPSNVNLTVTREG
                     DVLHGPGVGDNTVSVASVAMVGRALRDLGIVPAVDIVVTGNVGEEGLGDLRGMRAVVD
                     ALPNLGGAIAVEGHSLGRITHRAVGSRRLRVTVTGPGGHSWGQAGLPSAIHHLARIIA
                     RLDAIPLTDDPKTSFNAGIFQGGISVNTIAPEAVAVLDMRSTSAEALAGLVSEVERVL
                     ATPAPDGIDVDVEVVGNRPAGELPADRGLVPVATSVLRELGFDVAYDTSSTDANIPIS
                     RGIPAICIGLTTGGNVHRVDEYINISPLAKGFAQLLLTATRSADAIATGQL"
     gene            21215..24112
                     /locus_tag="VGR29_02720"
     CDS             21215..24112
                     /locus_tag="VGR29_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEV2072531.1"
                     /translation="MPDSLTGAADGLEYVAIDVEATGLNPQTDEVIEIGLVVFTRDLE
                     VSRYCRTIRPKQQVSRDILQLTGITAEDLGTSPSFAEVADELREQIGSRPIVGQSVGH
                     DLALLASAGLKPANRTIDTYRLASALLPDLPNYQLGTIAGHLGHEISGEDKHRALHDA
                     LAAMHVFKALLDRIDDYDPSTLSQVATLARTAGWPEAPFFQNAARERREAPLFMADRP
                     PTVPLELQFLVSRDRPEPLRPTGSSEPVDIERLERLLGRDGPLSRVLEKYEPRPTQVT
                     MAKAVAGSLNSEQQLLVEAGTGTGKSLAYLLPAAMFAISRGERVVVSTATIALQDQLY
                     RKDLPDVHTALVEAGINEELHVAVMKGRQNYLCLKQWFAHSNDPVEDEHDASLRAKIL
                     LWLGQTSTGDRAELRLTTEEERHWRKFASERGRCTVGRCPYAGSNQCFFHRARYNALH
                     AHIVIANHSLVLSNAAEGRVLPSFERLVIDEAHHLEDEATRQLSFVVDRPAVDDAVKA
                     LARSDGAVQGGAIPIAAAVLTRLKDSTAIKHSDKARSLADEAVHSATQINSLTGELFS
                     RLSQLVGKPRFGGGAGYAQSQRITEQMRDSGSFVQAALIWEELEHLLRKIQEAGAWFL
                     QVMDEVSLPNDDTHPLVQQRDDAVVELMTGVDQLSDVMRQLHECLGELDPTRVYWVQR
                     SGQLGLISLNGAPLDVSVLLNQYVYASLRTVVLTSATLTIDGSFSFIAEHLGLLDATT
                     LDLGSPFDHENTTLVYVPEDMPEPNHPNYTSVLHDVLGEALIGTQGRALVLFTSHRAL
                     RDARNALKEPLESHNIIVLGQGVDGSSRNLIERLRTEEGMVVFGTSSFWEGVDVVGDA
                     LSMVIITKFPFSVPSDPIFEARSELYDQPFMELSLPLAVLKFKQGFGRLIRSAEDRGV
                     CAILDKRAVSKRYGSSFIQSLPPCHVVVGSSYELPDEAARWIAAAPARVGQ"
     gene            24130..25119
                     /gene="glpX"
                     /locus_tag="VGR29_02725"
     CDS             24130..25119
                     /gene="glpX"
                     /locus_tag="VGR29_02725"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEV2072532.1"
                     /translation="MDNPDISRNISLELARCTEAAALMSARWMGRGDKETADQAAVDA
                     MRQLLNTIDMDATVVIGEGEKDEAPMLYVGERLGCADAPRVDIAVDPVEGTRLLAQGQ
                     PNALCVIAVAERGAFYGWQDIAYMEKIAVGPSAAGRIDITRSVTENLIRIADAKGMKV
                     EDLTVVMLDRPRHEEIIRETRAAGSRIKLITDGDVAGALMTSMEGTGVDLLLGIGGSP
                     EAVITAAALKCSGGDMQCRLWPRNEHERELAQQNGIDLTRVFTIDDLVGGDDVFFAAT
                     GITDGELLDGVKYVPGGATTHTLVMRAVSGTVRYIRSTHFTRKLENLAAATKH"
     gene            25551..27221
                     /gene="rho"
                     /locus_tag="VGR29_02730"
     CDS             25551..27221
                     /gene="rho"
                     /locus_tag="VGR29_02730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEV2072533.1"
                     /translation="MTLEDTLTQEKTTEAVAEKPVRRTRTVRARRKVDEDAAGSNGTS
                     PADVPETAPAIVQEAPVAPEVVVATPTVPVVQPVAETPAEPQDGQQRQRRNGNQRGNK
                     KRNNKGPEQNGQQGGDLQQARNNRPQQNRERQQEPAGDRLTVASLDARADDALHDLAR
                     ELGVSGYLRMRRPDLITKLLQAQTERDGHIFGDGVLEIIEDGFGFLRGDRFLPGPDDI
                     YISQSQIRRFGLRTGDRVSGQVRPPKDSEKFFSLLRVEAVNGIDPETARRRPAFDKLT
                     PIFPLELIDLETASNVLSTRLLNLVAPIGRGQRGLIVSPPKAGKTLLLKSIANGITSN
                     NPDMHLMVCLIGERPEEVTDMRRSVDGEVISSTFDEPVEDHTKVAEMALERAKRLVES
                     GRDVTILLDSITRLARAYNLAVPPSGRTLSGGIDPVALYPPKRFFGAARNIEGGGSLT
                     IVATCLVDTGSRMDDVIYEEFKGTGNMELHLDRKLAERRIYPAIDIQRSGTRREELLL
                     SDSVLRQVWTMRRMVSVLGGTEGTEALLGRLSKTQSNAEFLEGLTRAD"
     gene            complement(27367..28317)
                     /locus_tag="VGR29_02735"
     CDS             complement(27367..28317)
                     /locus_tag="VGR29_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEV2072534.1"
                     /translation="MSGIVVASHNGRIGIDASVDVLRSGGSALDAVVAGIRLVEANPD
                     DHTVGYSGLPNLLGDVELDASIMEGSGLRAGSVGALQGYQDAVDLARYVMDTLPHVLI
                     GGEGAARLAREMGLEPKNLLTPEAQEIWRSRLEDQTDRDASYLGRIQELVAKTAEDPE
                     IAEQLGEPAHGTVNFIARDRHGTIACGVSTSGWAWKYPGRLGDSPIIGAGNYADNRWG
                     AAACTGRGEMAQRCVTAHSVVTFMRFGMSLQEALHHAMKDLEHLDDPYASEMNIVALD
                     RDGNPGAASTNPDKTYVVMTEEMSEPDERPRTHVDISMVR"
     gene            complement(28314..28589)
                     /locus_tag="VGR29_02740"
     CDS             complement(28314..28589)
                     /locus_tag="VGR29_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEV2072535.1"
                     /translation="MSDNRPPIILYGRQTFCPDVARAKLRLEELGHEWIEYDIECESK
                     RRQEVLELTGQAKVPTLVIGDKVLVEPATAQLDAALLAAGYEMDDTE"
     gene            complement(28618..29586)
                     /locus_tag="VGR29_02745"
     CDS             complement(28618..29586)
                     /locus_tag="VGR29_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEV2072536.1"
                     /translation="MARLISQYRTPEDASVIVQPKYGFDAAAVLLGAETLLVKSDPIT
                     FATDGAGHYLVAVNANDIACMGGIPRWLTVVVLLPEGKTTPATVERLFSELREACVEA
                     GVALIGGHTEITLGVDRPLLVGTMLGVPGPTGLLRPGEASPGDELYVTKWIGIEGTAL
                     LGHDCDTDLAALGENVIERAKLLLRDPGVSVVPDALASLASGVVTALHDPTEGGIATA
                     IHELAEASSLGALVEHDSIPVREETRLICDQLSIDPLGLLSSGALLIAAKPGTGEKLR
                     ETLEAASIPVTRIGRLTDIRHGVQLIVGDETVPLRRYDSDEITRVL"
     gene            complement(29642..29869)
                     /gene="xseB"
                     /locus_tag="VGR29_02750"
     CDS             complement(29642..29869)
                     /gene="xseB"
                     /locus_tag="VGR29_02750"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV2072537.1"
                     /translation="MIDTRDGRAEEQISFEDALQKIREIVSALESGNLTLEESIANYK
                     AGAHLIDLCRSLIADAELRVTELAREDEADQ"
     gene            complement(29866..31104)
                     /gene="xseA"
                     /locus_tag="VGR29_02755"
     CDS             complement(29866..31104)
                     /gene="xseA"
                     /locus_tag="VGR29_02755"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV2072538.1"
                     /translation="MLRIVSIDQLTSLLKVLVEDDDDLQDLWVDGEVSNLSVARSGHA
                     YFTLRDETSQLSAVMWRTALARQKMIPREGDRVIAHGSMTFYQPRGNLQLQVDLLQPQ
                     GTGLLQLRLEELRQKLEAEGIFEPSRKRPLPLFPGRIGVVTSATGAVWHDIQQVVGRR
                     FPLAHLILSPASVQGETAPDSLVKALVCLQEVAEPDVIIIGRGGGSLEDLWAFNDERV
                     VRAIFASRVPIVSAVGHQTDMTLSDYAADVRAPTPSAAAEMAVPDLSAIDDALNEAQV
                     RIRVEIQDRLEQSLQVLDDMQARLARVSPTAAVTSLRRDLAPLEDRLHLAIRQRLQFA
                     RHDTDAFHEVLEALNPHALMCRGFSFVCDVDTGSPVREVSQAWPGTSVRAVLADGELT
                     AQVTDVRRHSSPLAGRDVSE"
     gene            complement(31104..31586)
                     /gene="accB"
                     /locus_tag="VGR29_02760"
     CDS             complement(31104..31586)
                     /gene="accB"
                     /locus_tag="VGR29_02760"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV2072539.1"
                     /translation="MTGQDDRNTPPSFTIDGVIEAVRELISMMSKGGISELDLSTGDV
                     TIRLRGASSTLVATQGVTSTESVMAALPQSDPEGHLITAPMIGTYYSAPAPGEAPFVQ
                     LGDEIEVGQVIGIVEAMKIMNEIVADYSGVLVEVLVENAQPVEYGSPLMRVSQTDSLG
                     "
     gene            complement(31705..32265)
                     /gene="efp"
                     /locus_tag="VGR29_02765"
     CDS             complement(31705..32265)
                     /gene="efp"
                     /locus_tag="VGR29_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV2072540.1"
                     /translation="MIDTGDVRKGVTLDIDGKLVKVIEFSHNKQGRGSAQLRMTLRDL
                     RTGSNTQMTVMAGTKFQLVRLERQHVQFLYAEGDQFHFMDTETFEQIALDRKAVGDAE
                     KFMKENDVIDLLTYNGEPIDVELPTSVVLAVTQTDPGFKGDTATGGNKPATLETGLTV
                     NVPLFINEGDQLKVDTRTGEYIERVI"
     gene            complement(32262..33377)
                     /locus_tag="VGR29_02770"
     CDS             complement(32262..33377)
                     /locus_tag="VGR29_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV2072541.1"
                     /translation="MTNDRLARAGALLNTLDLDAVLITNGTNRRYLTGFTANDHAPDE
                     LSATAVVSPDEVMLFAPSTNLPWAESEALPGVMVRPAERKWTDSIAELVKTSGWTKFG
                     FEDATTTVAVYNELHQALGGQVDLVPVGDATDSLRAVKDESEITLLTNALRLTDQAFM
                     SAETRIQAGMTEKQAAEIVAEELRRAGSDGEAFPTIVASGPNAAKPHHAPGDRIIEEG
                     EPIIIDLGALHFGYAGDLTRTIWFGQPSGQLVTMYRTVQAAHDAALAKAAPGVTGIEL
                     DSAARDVFTAQGLDHYFVHSLGHGLGLRVHEAPSASMRSRDTLEPGHVVTIEPGLYIP
                     EWGGVRIEDVVLITEDGAQNLTSSPKRSVEATQEKNA"
     gene            complement(33374..33856)
                     /gene="aroQ"
                     /locus_tag="VGR29_02775"
     CDS             complement(33374..33856)
                     /gene="aroQ"
                     /locus_tag="VGR29_02775"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEV2072542.1"
                     /translation="MLASSAASTILVLNGPNLNMLGTRQPEIYGRLTLAEMMDNLQRA
                     AASGTPALSLLHYQSNHEGVLIDAIHDQGPDAFGIIVNAGALTHYSIALRDALTSIET
                     PAIEVHISNVHAREDFRHRSVISAVVTGQIVGLGEDGYHLALEWFRRQAKRIEEKSFR
                     "
     gene            complement(33860..34843)
                     /locus_tag="VGR29_02780"
     CDS             complement(33860..34843)
                     /locus_tag="VGR29_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072543.1"
                     /translation="MPFTSTRRSSFLSMLILSATLTLSTAAPVLAQDGSTPVPGSEIS
                     SETTSQLPAADLPTMNQQGFVFELESTYDGAFTQLPEEAPVYTMRFPQVDAERAKTLA
                     GNLGIDGEVETEDNATFDVQDESGSLFIVPGMMQFISFAEIPQGDLPTDEQGIAFARE
                     WLRQVNLLPADVGEGKVVTRIEDPPRIIVSFQPITPSPLLSATPNITVTLGPEGAVLE
                     SSYRWADISQSDLYQLRGTSAAWTEVESQRSYLETLLPSDQYEPGAMITGVATYDQVS
                     LAYTSSGIPGEQQYLQPVYVFSGQVTPEGSDTSYPITSYVPALINSQQPVG"
     gene            complement(34891..36906)
                     /gene="uvrB"
                     /locus_tag="VGR29_02785"
     CDS             complement(34891..36906)
                     /gene="uvrB"
                     /locus_tag="VGR29_02785"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEV2072544.1"
                     /translation="MPDFKIVSDLRPTGDQPTAIAELTEGLKAGERFQTLLGVTGSGK
                     TFTMANVVERVNRPTLVLAHNKTLAAQLYSEFKEFFPHNAVEYFVSYYDYYQPEAYVA
                     RTDTFIEKDADINEEIDKLRHAATRALLTRDDTLIVASVSCIFGLGTPEEYAKTMISL
                     RRGQQIRRDKVLRQLVDIQYDRNDMTLIRGSFRARGDTLEIYPAYEEIAVRVEFFGDE
                     VERIVEVDPLTGELLTEREDIAIYPAKHFVTTNDMMNVAIKDIEKELQDRLKELESQG
                     KVLEAARLKQRTMYDIEMMQEAGYCAGIENYSMHLSRRQFGDKPSTLLDYFPSDFLMI
                     VDESHITLPQVRGMFGGDRSRKEVLVEYGFRLPSAKENRPLTFPEFEKIIHQAVFVSA
                     TPGPYEYEHSSRIVEQVIRPTGLLDPAVSVRQTKGQIDDLLAEVNARVAKGERALVTT
                     LTKRMAEDLADYLKEMGVRTHYLHSEIDTFERIEILRDLRLGIHDVVVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSEGSLVQTIGRAARHVDGQVIMYADTMTRSMKAAIDETY
                     RRREIQIAYNTKNGIEPRGIVKQIKDLTERVRAVAEEHAEYSTDGAKGSGVISQIPRD
                     ELARMIKDLESQMKTAARDLEFEKAALLRDQIVELRRIVEVDPVKAF"
     gene            complement(36976..37620)
                     /locus_tag="VGR29_02790"
     CDS             complement(36976..37620)
                     /locus_tag="VGR29_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyltransferase"
                     /protein_id="HEV2072545.1"
                     /translation="MPQVLSDIVDVYVFRRLNARVQFLLLQRRSDVALPHTWQAFHSQ
                     VRVGDTTLQTITETVKSLAGLNVSAVYSADYINQFYDEGRDVIVLAPVFAVNVSPQSP
                     IDLGDDYRDAAWFGRDEATARLPFSGQRWAVRHIDEIMSLGEAETGIYQIDMATIQPS
                     QQAAGSRDDLDDDDQVTEEFDGASLATVASVSEAKYQPPESHQGDSASKEEEGT"
     gene            complement(37680..38165)
                     /locus_tag="VGR29_02795"
     CDS             complement(37680..38165)
                     /locus_tag="VGR29_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072546.1"
                     /translation="MPATTDPPEKPEGHRPKTRLERQIEEILERAERDNPLPPPISFE
                     HEKRKRTPNPLTSPRVQNIGSSAHRWLTVSPFVVAFLLAIVARVVNEVSPFIAHLAVS
                     AAVIAFFWPILESIRNKQSGTPTATMWRGRDMSDLANRREYPTPLEQIRQWLRDRRPR
                     P"
     gene            38283..38792
                     /locus_tag="VGR29_02800"
     CDS             38283..38792
                     /locus_tag="VGR29_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072547.1"
                     /translation="MSSQSAVQSRSQFRCNVIDHKGTVSFVAPPHGLKVIAASIASGA
                     SSFAELIAHARRFDSAWAESVNRDLNRFDEHNVETVGLWFDTALSAEEDTRRQSFRVI
                     DQTTRRQSLVPGGLGLVVFNLKEQRIIQVQNSYEDLRRKDRGRVRINGKPTRRLFQYE
                     LPSGWAIVP"
     gene            complement(38928..39935)
                     /locus_tag="VGR29_02805"
     CDS             complement(38928..39935)
                     /locus_tag="VGR29_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEV2072548.1"
                     /translation="MQQISILGLGLIGGSIGLGLRRWSAQHENALRVIGYDSDMDKQT
                     LAKKMGAVDDTTWSLADAVRPADVLIIATPVGSMREIFSDIAPHLKQDAIVTDTGSTK
                     VNVLEWAAQLPAHVSFVGGHPMAGKSESLEAADADLFRNATWVVCPGVTASEAAIKNV
                     LGLVAAVEAEAFFADPAEHDSYVAGVSHLPMVVAAALVRTATADQSWRDMRTLAAGGF
                     KDTTRLALGSPEMHRDISLTNRAAISRWINQMIVTLEDFRAVLALEDEKAAGEQIYKF
                     FSEAQEQRARTEVVQSRASERNPESAGNLTSEKVSDSMGRMFLGGLGRRDKNKGKKWT
                     G"
     gene            complement(39948..40523)
                     /locus_tag="VGR29_02810"
     CDS             complement(39948..40523)
                     /locus_tag="VGR29_02810"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2072549.1"
                     /translation="MTDPGLNHRLRQRSRRAGIMIGISMLLTIAVCVGSFSIIYAQLD
                     TIVGDFVTRNEPNQARATEPAVVAAAAPTQPPSSEPTTAPAPTNAPTEAPAETTEAET
                     TEEPDEDEFDPDYQIDSVGSVNLRSGPGTDTPAIRALPLEEPLLYLGESQESDNPERD
                     GLAEGQVWMHFRTEDGEEGWVRELDVTEYVP"
     gene            40848..>41578
                     /locus_tag="VGR29_02815"
     CDS             40848..>41578
                     /locus_tag="VGR29_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV2072550.1"
                     /translation="MKSDLYTAIAQIAAERGIPREAVLSSVQQALTAVYRKNANSEED
                     VRVELDQATGEMQVVVVKTVVEQVTDPDLEISADDARAFAPAPILGDQIKIVRTPENF
                     GRIAAQTVKQVVHTRIRDFERETVYKEYQDKEGEVLSGVVQRADNRAVIIELGKAEAV
                     MPAREQVPTERYRPNQRLKVLLTEVNAHARGPQLIVSRSDARLIRRLFEQEVPEIQSG
                     AVEIMEIAREPGLRSKVAVAARQEKV"
BASE COUNT         8416 a        12027 c        12555 g         8580 t
ORIGIN      
        1 ccttcgaccc tgagccgaca ttcttcgagc tcacaggcca gacagtcctg ttgctcggca
       61 cgggcgccat cgggaaggcg acggcggagc gcttgcgtcc cttcggatgc acgatcattg
      121 gtgcgcgacg aactcctcaa gtgttgaacg gatttgattg ggttgttggt tttggagaac
      181 tgggagacat cctgccgaat gtggaccacg tggtcagcag cttgccgatg acgcccggca
      241 cggcgaagct cttctcgtca gagatgatcg ggagaatgaa gaggggagcc tacttcttca
      301 acgtcggccg cggcggcact gtcgatcagg aggcgctcat cgaggcgttg gtgaacgggc
      361 atctcggggg cgcgggactg gatgtcactg atcccgagcc gctccccgtc gactctcccc
      421 tctggagcat gccgaatgtg ctaatcacaa gccacacatc tggcggctct cccaaagcgg
      481 atgaacgcgt ggcagaactg gcgatggaga acatccgccg gtattgtgcc ggcgaggacc
      541 tcctgaacct ggtagatctg gagcacggct actagcacgg ggcaacggag ggataaagcg
      601 gttggctgtt cgtcccctcc taaaaccgtt caggcatctt cccgatgtgc gcctggtccg
      661 aatacgcacc gctgcaggac tgttgtatcg gtcgccattt ttcgagagtt gtagaactca
      721 cgctcaagcc tgttgctgac tgagctcact gcatccttgt ggaggagcgc gagcgcgggc
      781 ccgccttccc ctcctcccat catcctcgct cccaagacgc cttcctgttc gcgcaggagg
      841 tggaccaatg cttcgaccct tgaatgactg atctcgtagt ctctggcgga gctttccccc
      901 gatgcgttca tgagccggcc gaatcgactc cagtcccctg cgcgcattgc ggtctcggct
      961 ttgccaacgc ggcgcgtctc ggaaacgaca tgtcgaactc gcttcaagag cgtttggttc
     1021 aaatggctgt ggatggtcgg catgacattg tcccagtgag gggtaagggc gctgaggtgg
     1081 ggaagatcaa ggtcgtatgc gtcagccaat atttgtcgtg cctcttctgt ttcacggacc
     1141 cgagcaccat aggaggatgt tcgcaaggat cgaccgacac cggagctggc aaccgcaaaa
     1201 acgaggtcgc ccaggctggg actgaggcgg gagaacgaat tatcgcgacc atcgaaccgg
     1261 ataacctctc cggcaacaga ggcagactgg tccatggcgc ccactgggga gccggcgcga
     1321 tgctccgctt cgcgggcgat cgccaccatg tcgagaggct cgatttcatc aacggtcagc
     1381 gcgagaagcg ttgcgatgca aagggcggcc gaggagctca tgccgaagcc tcgagggacg
     1441 tctccgctta tcgcgagccc tactcctgcc cggaaaggga taccgcgggc gatcagtgct
     1501 gcaacgaccc ccttgagata gacgaccggc ccgctgtctc cttccccggc acgccagtca
     1561 ccaagcgtga gggcatcgaa cgtgtgcctc tggccattca cgtccgcggc gaccatacag
     1621 atgtcgcgcg gagcgttgag attgccgctc cccaccacga caacccgatc gatggctgct
     1681 gcaaggaccg gcccgccgtt gtaatcgatg tggttgccga taagctcaat ccgtccagga
     1741 gcaatagccc aaccatcgac cagccagtcg cagccgaatg ctgcgactcc ggcccggacc
     1801 gcacggcgtt tcagggagtc gtgtgacatt taccggccgc taacggttca tcttcacgga
     1861 cttaaaggct tccgcgagct cgaaaggctg atccttggcg agttcccggg atcgttccct
     1921 aagccgctcg tcaagcgtgg caccatcgcc gacctgtgaa acgtgtgcct tcagtgcctc
     1981 gatcttattc tcgaaggtgt ccgtgatatc gacaaagaca tcaggcgcct ccgatccgaa
     2041 gtaccagatc tctgccgtct tgtggggctc gataccttcc gccagatgat ccgggaagta
     2101 gagatgatcg cgagcggtgg gatagacgga gtcgtgggtt gcttgaccga cgccccgatg
     2161 atccggatgc atggcatacg gtcggaaggg gtcatgcgta atcactacgt caggcttgtg
     2221 gatgcggatc tgcttaacga ttttctcccg gagaacaatg tccgggacga tgtggccatc
     2281 gccctggcgg aggaagacca cctcatcaat accctcacgc cgggcggcct ctaattgctc
     2341 cttctcgcgg gtcgctccca gcgtctccgg cgtctgcgag cgatcagccg taccgcggtc
     2401 accggaggtt acctggatga ggacgatgcg cttgccctcc cgggaaagct tggtgatcgt
     2461 tcctgatgag gagaactcgg catcgtccgg gtgcgcgacg acccacatgt aggtctggta
     2521 gtcggatcga tcctgtccgt ctgtcatgtt cgtcggattc ctctgtcgtg tcgcgcggca
     2581 ggcgcatgcg tgcgaagcat caagccttca ggaggtgaga gagttcctgc accaccatcg
     2641 gtcgagcgac catcgactgc tcgccggttc tgaggttctt gatcgcgact tcatcacgag
     2701 ctaattcatc aggtccctgg atgactgcga aggggatgcc acgccggtct gcgtacttca
     2761 gttgatcacc catgcctttc ttcgtcaata cagattggtc aacatggata cctgtttccc
     2821 ggagatcccg ggcaagtgca gaggcgtcgg ccaatgtctc ctcacggtga accagaaaga
     2881 catcggcaac agattgacgc cgtggcacca aatcgaattc ttggatgacc tcaaggatcc
     2941 gctctatgcc aagggagatc cctgtggcgg ggatgtcgcg cccgagaaac gttccgacga
     3001 gcccatcata tcgtcccccg ccggcaacag atccgacctg cagttcctcg acaactgcct
     3061 catagacagg accagtgtaa taatctatcc cccgcgcgag ggccagatcg atcctgtacg
     3121 cattcttgtg gacaccgagc agagcgatgt gatcgatgag ctcgcgtagt tcacgtactg
     3181 cctggacacc gtcaggatag tcgtgaaggt actcgtgggc gaggtccagc aactcctcgt
     3241 tggtcccgct ggtcgtgacc acgtccagta cctgatttgc agcctcctcg ctcacggcac
     3301 ctctcatcaa ctccgacaaa acgccgtcgc gcccgacctt atccagtttg tcgatcgcgc
     3361 gatagatggt cggtgcctga tggggagaga cacctgccat tgatgcaagg gcttcaagga
     3421 gcttccgatg gttgatgacg acgactgcgt gaccgaggcc aatggcgcgt agggcatcgg
     3481 tgaggacact gatcgcctcc gcatccacaa gtgccgatcc ggggcccaca atgtcggcat
     3541 cacactgcca gaactcccgg aagcgacccc gttgcggctt ttcggcccgc cagacgggcg
     3601 caatgtggta gcgcttaaat ggcagcacca ggtcgttttg atgcatggcg acgacgcgcg
     3661 ccaaggggac agtgaggtcg tagcgcagtc ccacctcccg ttccccctga tccgtaaagt
     3721 gatacatgag tttttcgttc tcacccgcct tgccggtgag aatctccata tattcgagcg
     3781 cgggggtgtc cagcggctcg aagccgtgtt gctcgaagac ctcccggatg gagcccatta
     3841 cctgctggcg cagaagcatc tgctcgggga ggtaatcacg gaagcctttg agaacctggg
     3901 gtttacgtgg tgccaaattg gtctcctgta cggtgacgga tgatgagagc tacttcgcta
     3961 tctcgtggac aacgtgggcg agttcggggc cgatcaactt ctccatcgcg agtctcactg
     4021 cattggacga tccgggggta gcaaaaatgg cggtgttgtt ggagacacca gcgaccgccc
     4081 ggctgagcat cgcggcggcg ccaatctccg caaatgaaag catccggaag atctcaccga
     4141 acccgtcgag ccgcttttcg agcactcccg taatcgcatc gtacgttaca tcgcggccag
     4201 cgatgccggt tccgccattg gtcacgatcg cctggatgtt gtcgtcagca atccagcgtt
     4261 ccagcacctc cgtgatcctt gagccgtcgt ccggcacgat ctcgtaggcg gtgatggtgt
     4321 gacctcgcca gtcaaggtgc tgccgaataa gctgtccgct ccggtcgtct tcccgcgtgc
     4381 gcgtgtcgct gatcgtcagc acggcgcagg cgacgctctt tggcgcgtcg gcgcgatgtt
     4441 cgtcggtcga cgccgaattt gccatgttcc cggacggctc aaatggggag cgcatcgttc
     4501 ttccttccag atgggtgttc gcgatgtcgc agtggcgtta ccacctgttg caccgctgga
     4561 tatggtatcc gattgcagga gccgggagcg tcagtctcta acccgtaacg atggcatttt
     4621 cataatggtt ggcttgcgcg gcacccatct tcggatcgat cgtgattgca acgaggtcgc
     4681 accgccaggt ccgatcctga tgctccggat gttcgccaaa gtaccattcc gctgtggcaa
     4741 gcattcggcg ggcttttgcc ggtgagacgg catcatcggc acggccagcc cgctctcctc
     4801 gtcgcgtttt gacctcgacg aagacgagaa ggtcacggtc gagcatgacg aggtcgagtt
     4861 cccctgcact gcagtgccag ttggacgcga ggaattgata gccatgcgcc tcgagcatca
     4921 ttcgcgcatg tcgctcaccc gcgataccaa tagcgtcttt atttcgcctg gcgggcatcg
     4981 acgagctcct gggcataacg cactggcgcg aagcaacggc gatggaggac gcaaggacca
     5041 tgctgtcgga gggcggcaat atgggagcgg actccgtaac ccttgtggcg agcgaagcca
     5101 tagacaggcc acgtcctgga ggcgtcgacc atgagagcgt cccgcgtgac cttggccaca
     5161 attgacgccg cagcgataga gaggctcaga gcgtcgccgt cgataatccc tatctgcaag
     5221 gtgctgctct cgatcgtcat ggcgtcgatc aggaggactt ctggctcgat gtcgagaccg
     5281 agaacggccc gctccatgac aatacggttc gatggcccta gcccgagctc gtcaagttcg
     5341 tccggctcga catcagctgt cgaccaggag ggaacacgag atttaattcc ttccgcaata
     5401 gccgctcttt tggcctccgg gatcgttttc gaatcacgaa cggttcgcca gaaatcgaca
     5461 tcctgcctca ggctctcggg ggtcggcagg ataaccgccg ccgcgacgag aggacccgca
     5521 agggcccctc gtcccacctc gtctacgccc gcaatcgcta agatcccctc tgaatggagc
     5581 gcaagctcat acgtcagggt cgaaagttcg ggtgcgatat caagcattta gcgaacccga
     5641 tcagcgacgc gcacggtgtg gcgggtaaca tccgcgaggt catcccacct tccgatcgac
     5701 gcgttatagg cttggtgact gagaccgatg tgaagttgat agcatagaag gcgctcatgg
     5761 aagttatgga ggtcgacgcc caggtcggca aagaagcggt gtgcctccgt tgccacgctt
     5821 attccctgcc atttgggata ccacggagac caaaattcga accaggcgag ctcgtacagg
     5881 aagtcgccgt acattgcaca gccccaatca atcacgcccg agagatggtg atcgctcaca
     5941 aaggcgttgt aattgaggag atcgctatga atcacgtgtc gcacattggg catgtccttg
     6001 agcatccgtt cgagcacaat cacgtcacga tcaaaggcat gggctccagt cggtgattgc
     6061 tccaggcgcg gccgccagcc tccaccgcgt tgatccggag agtcctccag cgacgcccgc
     6121 aagtgcccgg gccacgaggt tgccattccg ttgccggacg aatcccagcc tccatagcca
     6181 gttgaggagg tgacgtctgc tatccgaagg gcgttcagtg tcccaagaag tgacggtagt
     6241 gtccgttcat agtccgctct gtctagctta tcgaggacgt cgccgggaac gcgctgcgac
     6301 accgcatacg agatcccgtc cagggaacct cgatgtgtca cctcaggaac agggaggtcg
     6361 ggtgaccaga atcgaaatgc gtaagcgtca cggtcgaagt cgtctccatg attactgaac
     6421 cgaatgaccc atcggcggtg accttcagca taggcaaata cggacgacca tgctcccggt
     6481 cgaagtgttt cgactccaga gatgtcgaga cctaatctct cccgcagaag ggtcaggacg
     6541 tgcgtgttgg agatagccgg gggaatcatc gcaggtatcc tggtaaatga tgtgacgcat
     6601 gggtcaggga ttgctggcag aaacaaaacg accgaaggca ctggactgcc ttcggtcgtt
     6661 tccgatattc gctcgagtta ccggttgtcc gtgctacttc tgctcgtcct cagtgggctc
     6721 gccctgcgga gcgtcctcga ccgggttggt tggctcctca gcgcgagcgg cctcatcagc
     6781 cgccgccttg tcgtcaacga gctcttcggt ctctggctcc tgcgcttctg cttccgcggc
     6841 tgcagcttcg cgggccgcga gctcggcggc aatccgctct tcctcaagcc tcgcggctct
     6901 ttcggactgc ttccgggcgg cagcggtcag gtcgcgacgg cgctcggtaa tacgggcggc
     6961 acgaccggtg agcccacgca ggtaataaag cttggcgcgg cgaaccttgc cgaagcgggt
     7021 gacctcgatc ctgtcgatac gaggggagtg aatcaggaac gtgcgctcga cgccgacgct
     7081 atgggaggcg atacggcgaa cagtgaaatt ctcgttgata ccaccgccac gacggcgaat
     7141 caccacgccc tcgaaaacct ggatccgctc gcggctgccc tcgacaacct tcgcgtgcac
     7201 gcgcaccgtg tcaccggcgc taaactgcgg aacttccttg aactgctccg cttcgatgga
     7261 gcgaattagt tgggcaacca tattcctacg ttcctcgtgt attaccaaaa cagcgtgtaa
     7321 tggatctgaa cgcgtctaat attgcaggga gaagtgcaca tggtctctgt cccgagatca
     7381 acaggcaccg cgcgattgta gcatgctcct tcgctgccag ccaatcgctg ggttatttgt
     7441 ctcagtcctc aagcacctgg atgacctccg ccgcgacctc gtaacgcccg aaggaaggca
     7501 tgagatagac gccctcggca tggggacgca actcatggag taactcctgc gccatcttga
     7561 ccccttcctt gcggcccgag ttaccagcct tgcgcatccg ttcccgcgcg tcgtcggtca
     7621 gggtgatgcc aggtacttcg ttatgaagaa actcggcatg ccgtgagctt tgcagtggca
     7681 ggatgccaat catcacaggt accggaagcc ggtctacacc gtagagttcc aggaagttgg
     7741 tccagacgcg catatcgaag atcggctggg tcataacgaa gtcggcgccg gcgtcgatct
     7801 tctcccgcaa ccgacggacc tcttcgtcaa gatccggctt tgtcgggtcc accgcgcagg
     7861 cgattgtgaa gttggctgcc cgcccgatgg acgatccgga cgagtcctct ccctgtttca
     7921 ttctcctgat tacccgaatc aggccgatcg agtcgacgtc gtagacagcg gatgaatcag
     7981 gataatcacc gaggctgggc ggatcgccag tgagcgcgag gatgttccgg acgccagcgg
     8041 catgggcgcc aagcagctct gactggagtc ccatcaggga acggtctcgc gtggtgaaat
     8101 gaataattgt ttcgataccg atctgatgtt ggatcaggta gcagagcatc atcgcgctca
     8161 tccggacgcg cgccatcggg gagtcggcga cgttgatcgc gtcaacgccc gcttccttca
     8221 gcatccgggc tccgtccagc gccttctgcg gattcagacc cttcggtggg tcgatctcca
     8281 cgcacttcag aaatttcttc cccagtgctt ttttgacttc cgtatcgtcc tcatcgcttt
     8341 cgggaatcgt gatcactgct acctgcgggc cggaattctg ggacgctcgg ccgttagggg
     8401 aagggcggtg atcggcctcg ccgctgtcaa tggcatgccg catggcattg gtgtgcgccg
     8461 cggttgtgcc acagcaaccg ccaatgaggc gggcgccagc gatcatcgcc tcttgggcaa
     8521 attgggcgaa gtactcaggc gacgactggt agatgagccg ggaaccgacg cgggagggcc
     8581 aacccgcatt cgggactgca gctatccgcg gggtatcttc cgcagtagcc gtattcgaga
     8641 gttcctcgtg catccgccga atcaccccca gcagccgttg gggacccaca gaacagttgg
     8701 caccgacaac gtcgactcgt tgttccgcaa ggtggcggat cacctgttca gggctggcac
     8761 cggacagggt gaggccatcc tcggcgaagg tcatcagggc gatgatcggc aggtcagacg
     8821 cctcccgtgc tgccgcaatg gcagcatcca tttcattgag gtcgcccatc gtctcgatca
     8881 tgagaaggtc gacgcctgcc tccagcagcg ccccgatctg ctcggcgaac gcctcctgtg
     8941 cctcttcctt tggcaaggta ccgtagggct ccagcgttct ccctgtgggg ccgatggaac
     9001 cagcgacgag caccggtctg ccgctgatct cgcgctcctc ccgggcgatt ttcacgccac
     9061 ggaagttgat gtccctcacc ttgtcggcca ggccgtgcag ctccaaccgg aatctgttcg
     9121 ccccgaatgt gttcgtttcg atgatgtccg cgcccgcgtt gaggtagctg cggtgcacca
     9181 gggcgatggt ggctgggtgt gtgagattca gggcgtcaag acatgtgtcc agctcgtgcc
     9241 cggcttcgta gagcattgtg cccattgcac catcgccgag gaggacgccg gcatcgagac
     9301 gttcgagtag gggatgtgcc atgaactctt tccaacgagc gcttcagcgc gatagccaag
     9361 cgtaaaaact ttcaggtaac caccggcaag ccagggtagt gcggcatggt ccgataggtg
     9421 gctgatccgg tgttgtggtt accgtagcac aggaaatggg gggttccgat tgagatttgg
     9481 accgggaggc attggctcaa ctgtgttgac gtttcgccgg cactcgcggt acagtccttc
     9541 gcaattgacc gcacaccatc gcgtgcttag ctgatctcgg ttcaccgctg ttgcgcgatg
     9601 ctcaccccag gacccaggca aatatatgtc ttctgaaact ctgagtgcca gctccgaaga
     9661 gcagtccggc agcgtctcca accggcgggg catccttatg ccgatcggag gcgccgagga
     9721 caagctcgat aacaaggtga ttctctcgga gttcgtcgag catgctggag gcgagaaggc
     9781 tcgcatcgcg attgtaccga cggcgtcttc cattgaagcg gctggtctgc gatacaaggc
     9841 gatctttctc gggatgggcg tggagagcgc tgaggttgtc tatatcggga gccgggaaga
     9901 cgcgaacggc ggcgagagtc ttgatgcgat tgccgatgcc accggtatct tccttacagg
     9961 cgggaaccag atgcgcctct ccgccatcat cggtggtact gaattcgaac agctcgtccg
    10021 ggagcgccac aacgctgggg ttatcgtggc gggcacaagc gcgggcgcat ccattctctc
    10081 cgcccacatg gttgctctcg gggcaaacgg tgcgacgccg aagcttcgca tggcccagat
    10141 gttcgccggt ttcgggctca tctccaacgt cattatcgat cagcacttcc ggcaacgcga
    10201 ccgaattggt cggttgctct cgctcgtcgc gggcaatccc ggtttgctgg gtcttgggat
    10261 agacgaagac accgcactcg tcatcgatga ctcgggaatc atgaaggtcg tgggccggca
    10321 cagcgtcact attgtggatg gctcacacat ggtctccgac atcttccagg tcaagcgata
    10381 cggggagatc accatcagcg atgttcggct ccatgttttg ggccccggca ggaagttcga
    10441 tatgacgtcg cggaagatga ttcgcgaata gtattgacat ccgggcagtg aagcgcgaag
    10501 ttcaggcctc gccggacaat tggcggggca ttcgtgtata taggctgtag tacattcgtc
    10561 acagcgaaac tcaatcaaac agggccgttg atgcgccccg gaggtataca cggtgttgaa
    10621 tatccggcga accactgtct accgtgggcc aaacatctgg gcgcggatgc cggtgatcca
    10681 ctacgtgctc gatataggcg aactcgagga ccggcccacc aacaagattc ccggttttta
    10741 cgagcatttg accgagttga tcccgtccct gtatgaccat ggctgctcgc tcggtcgtcc
    10801 cggcgggttc ctccaacggc tgcgggaagg gacgtggatg ggccacgtgc tcgagcacgt
    10861 ggcgttggaa ctccagaatc tcgccggcgc cgaggtggcg cgcggcaaga cgcgccaggc
    10921 cggcgagcgc ggcgtctaca acgtcatctt ccagtatgag caagaggatg tggggcttgc
    10981 cgccggtgct ctcgcgctca ggctgctgaa ccacctcatt tatggaagcg aacccgattt
    11041 cgatttcgtg catgagcacg aggaaaaggt gatcaagctc gccgagcggt tcgcatacgg
    11101 cccctcgacg ggagcaatcg tccatgaggc gcaccgccgg ggaatccctg tgcttcggct
    11161 cgattcccgg cgatcccttg ttcagctcgg gcacggcaag tatcagcgcc gtatttgggc
    11221 gacggtgact tctgagacag ccaatattgc cgttcaggtt gcgagcaaca aggagctcac
    11281 caatcgcttg ctgcacgaga tcggcatccc ggtgccacgg tcgcagattg tgcaatcggc
    11341 cgatcaggct gtcagtgccg ccgctcggct ggggtatccc gtggtgatca aacccctgga
    11401 cggaaaccat ggacgcggag tcttcgtcaa tctcagcgat gagggtgaag ttcgcgagtt
    11461 ttatgactca gcacaagcgg aatcgcgaag tgggcagatc atcgttgaga cctttattcc
    11521 cggcaaggac tatcgaattc tcgttgtaaa caacgaggtg gtagcggcgg ctgaacgcgt
    11581 tcccgcacat gttattggcg atggcgagca tacggttcag gaattgatcg ctctcgcgaa
    11641 tcaggatccc cgccgcggtg tcggtcatga gaaaatcctc acgcggatca ccgtcgattc
    11701 ccagacgatg gaagtgctgg agcgtgacgg gcgaacgctg gagaccgtcg ctcaggacgg
    11761 agagttcgtc cagctcaagc tgaccgggaa catgagcaca ggcggcacct cgatcgatcg
    11821 cacggacgat atccatatcg acaatattga gatagctcgc caggcggcta tggtcgttgg
    11881 gctcgacatc gctggcatcg acctcatcac ccaagacatc tcgcgttccg taagggagta
    11941 cggcggcggc atctgtgagg tcaatgccgg accgggcttc cggatgcata cacatccaac
    12001 tgaaggtcac ccgcgccatg tcggacgggc tgtcgtcgat atgatgttcc ctcaagggga
    12061 gccgactcgc gtgccgatcg tcgccgtgac gggcacaaac ggcaagacga caacttctcg
    12121 catgatcgcg cacatcatga agacgtccgg aaaaacggtt gggcttacga ccaccgacgg
    12181 catcaacata gacgggacgc agatcatggc gggtgacatg agcggcccga cgagcgccag
    12241 gatggtcctc aaaaacccga cgatcgacta tgccgtcctg gagacagcac gtggcggaat
    12301 tcttcggtca ggccttggct atgaccgctg caacatcggc gtggtaacta acgtctccgg
    12361 cgatcacctt ggcctgaaag gcattgacac ccttgccgga cttgcacggg taaaggaagt
    12421 agtccccgcc agcgtcctgc gagacggtgc gtctgtcctc aacgctgaca acgagtggac
    12481 agtgcagatg acgcgatcgg cacggggcga gatcatcttt ttcagcatgg atgaggagaa
    12541 ccccgtgatc cgggatcaca tccggcaacg agggcgcgcg gtgctcctca agcagacccg
    12601 ccagggggag atgatcacca tctacgaaag tcgtcgcgag acgtcgttgc tgcttgcgag
    12661 ccagatcccg gcaacatatg agggccgtgt acgagtcaac atcgagaacg cgatggcggc
    12721 agctgcggct gcactcgccg cagacgtgca gctggattac atccggcttg ctctccgcac
    12781 cttcagcagc agcttttacc agacacccgg gcggtttaat cttctcgact acgggaacaa
    12841 gcggattgtc atggattact gccacaacgt cgcgggcctg gagtcgattg ccgatttcgt
    12901 gaagcgcatg gaggcagatg ccaccttggg ggtcatcagc atccccgggg atcggtccaa
    12961 tgacgatatg cgggcattcg gcgagctcgc ggggaagacc tttgaccgga tcatcattcg
    13021 cgaggacaac aatactcgtg gaagagcgcg gggtgagatc gccggtctgt tgaaggaggc
    13081 ggtcctgtcg actggaaagc cgtccgacga ggttgatgtg atcctggatg agcttgaggc
    13141 agcaaagcag ggtgtcgaac gagccaacag gggcgatctc gttgtgttgc tcgtggacaa
    13201 gcctgttgcc gtctgggaaa tgctaacagg cgatagaccg caatagattt ccaaggaatc
    13261 gaaagagagt tacgtgaatc aggcgctggc tgcttcggca accggcgcct cctcagttga
    13321 tcggtagcgc agaatcgttg gcacgaggcg aaccatggcg atagtgccca ggatggtgcc
    13381 cacaccgccg atcgcaactg ccgcaggtgc ccccatccag ctggccatgg cgccggcgcg
    13441 gaactcccca agctgcgggc cacccatagc gaaggcggaa tgagcggcgg cgatgcggcc
    13501 acggaaggcg tccggggtaa tgagattgcg aatcgcatgg cgcagtgcca tcgagatgga
    13561 atccgccgcg ccgctgcccg cgagaaagag cagggccagc cagatattgt tggtcaggcc
    13621 gaagcctacg atacaggcgc cgtaggcaat cacgcatgcc accatgccga acccgggttt
    13681 ttgcggcata gtgaagagac cgaataccaa actgccaagg attgccccgg ctgccggcgc
    13741 gctaagaagc aggccaagac tgttcgcccc acctcccaga atctcccccg caaaaatcgg
    13801 catcaggttg gtgatcgcgc cgaagaacgt ggcaatgaaa tcgagcgaca tcactgccag
    13861 caagataggg gtgtgccaaa gaaggacaaa accggccttg atagcagaaa ggccgcgttc
    13921 ggtgatcggg acccgtgcct gacgagtgcg gataagcaga gcggcggcaa tgacggcgat
    13981 gaaactagcc gcatcgagca tgtatgcgag ggcaagactc gtttgggcaa taatcactcc
    14041 cccgatcgcg ggaccagcaa ccatcgccgc ctgcatagag agattggcca tcgttgcagc
    14101 accggcaagg gaagatctgg ggacaagatt tgggatcagg gcctgacggg ccggtcctgc
    14161 catcgcattg aaggtggagg agatgaaggt gatgctgtag atgagcccga cgctgacctc
    14221 accggccagg gtcgcggcag cgagtgtacc cgtggatgcc aggagcgcca ggtgggtgaa
    14281 gatcaacacc atgcggcgat ctcgatgatc ggcgatgaca ccgcccagga gaccgaagac
    14341 caacacgggc acaaaccgaa acagacccag aaggcccagt tgaaacgggt cccccgtcag
    14401 ttcgaagaca tgccaagtaa tggctatgcg ctggatttgg gtgccgagaa tcgagacgct
    14461 gtcagcgaac cagagaagcc gaaagtcccg gaagcgcagt gtactgaagg cgtgggctct
    14521 ccggtctgac acaggctgtc tcattggata aggaagcgat acgataagct ggaacatggt
    14581 accgcgaagg cggctcgaaa ggtagtgtac gcaggatgca ggagagagta gtgggcttgt
    14641 caacatcagg aggagtgctc agccgggagc agctgaaggt gcttatcgaa gacccggatc
    14701 gccccttgtt acgggagtac gtggatctgg acggacaact gcagcccaac ggattcgatg
    14761 tcacgcttcg ggaagtgagc cgccattccg atggcggcgc gatcggtgtc acctccgagg
    14821 acagagtact ccctgatctc gagagactcc cgttcgacga aggcggatgg ctcaccttgg
    14881 cacccgggtc ctaccacatc ctctataacg aggtggtgga tattcctgac agtcttatgg
    14941 cgttgggcag accgcgttcc agcctcaatc gatgtggcgt cacaatccat actgcagtct
    15001 gggacgccgg atataacgga cgatcgacgt cgttgctcgt ggtagccaac tcgggcggat
    15061 tccgggttca gcgtaacgct cgcgtgatgc agttggtgtt cttcgggatg tcagaagcac
    15121 ctgccgacgg atatcgtggc agctatcagc tggaaaacat gtaacttgcg tctctacttt
    15181 gggtcgcgag ttcgggagaa aagtggccgg tcaggagccg cgcccgggac cgggatttgt
    15241 gtgcggcggt gcgtgcgcgt gtcggtgtgg ttgcagcctt cgacggggca gaagtagatc
    15301 agcgtgtact cttcttcagt ttggtcagcg aaccgggatg gacgcccgag cttgccgcgc
    15361 agacgcatct cagtgtgatg gtccggacag ttcggtatcg ccgggtcgtc agctttccgg
    15421 cgaaagatcg tatcgagaag agacggcatg gtacgttgct ccaacgctga attggtcgcc
    15481 aatggccaat agcgacagta tacgggatca tggatgtgta accgcgccag aatacactgg
    15541 tgcgttaaga atgacgggag aaggagtgag acatgccaga gaagggtcag acactccctg
    15601 caagcgtcta tgaggagctc cttgccctgt tgctcgaacg gttcggggag ggctgggctt
    15661 cggatgcgac ccaccacagg caattcggga catcaatgac gtccgtgatc cagacctccg
    15721 agggcatgca ccttattggc agtccggaga tcgagaagaa ctcggtgaac ttcaatctct
    15781 gggtagagca tccggtagag gacgtttggg aggccgacga gttggcgttt gccgtcttcg
    15841 ctcaagtagc ggaggatatc tttctttcgt cacggcaaat cgaggagagg ggcgttcgct
    15901 acaggtttgt cacgggcagt attgagagcg ggtatatcgg gagcctacat tttaccgggc
    15961 cgcacgccaa ggagctggca gacatctacc gcatgcgaat cacccaggga catcactatc
    16021 atgcctgaag ccaaaatgtg gactgttgac gtcgacggta tcggctccgt agaagtagcc
    16081 gaagggcgac ggctcgtgct cggcctcgag gacggcggga ttgatatcct gcacaggtgc
    16141 ggtggcaatg cacgttgtgc cacgtgccgt gtagaggtcc ttgagggcca accgacggat
    16201 atcaccgatg cagaagaagg tcgcctggac cggctaacga accgccagga tacgactcgc
    16261 ctgtcgtgtc agattcgggt gacggacaat ctgtctgtgc aggtttgcaa ccgactgtca
    16321 gagcagcctc agctcggtga cgccggtcct cggcccattg agtggccggc ggacagtgcg
    16381 ctacccccgg aagaatagaa cccgcgttag ggttgggttg gacctagggc ccgaacgggt
    16441 tataattaga gacggtcgtg ctagatgggg aactggcggt gccctgcacc cgcaatccgc
    16501 cacagcgggg ttgaattccc tctctaggtc cggcttttca ggactgtatc gtcacggggt
    16561 gttgaacact gggtcccgca cggcggggaa ctgtgaacct ggtcagggtc ggaagacagc
    16621 agccataagc ggcactcctc gggtgatgtg gggtttcctg gtgggagctc acggacggtg
    16681 gcaagctgga aggtcgcggt cgaaagaggg tgcacggctc tctttttgaa taaggggaag
    16741 gggcggtcca tttggatcgc cccttctgcg attatcctcc cggtccccga cgcgtatctc
    16801 gctaccgtcg tgcccgtcga gctggacgcc gcgtacggcg gtgagttgcc cggttgcggc
    16861 gccttgcttc ttcatccgaa ggtgctcgga tggcaaccat gatgatcgct agaagcagcc
    16921 ccaggactgt caggagtgtc cagattggga gcccaaggct caggggattg atctgcaagt
    16981 acctgataat gatcaggaag agcccaaacc cggagatcca cccgagaagc gttgacgccc
    17041 gctggatgaa tctccgcgaa tagcgttccc cgagtggctt ggctcgcggc cgcgcggaat
    17101 agaacgccgc gataataaat accaatgcaa acacggacgc cgcagcaagc gaagggatgt
    17161 tcaggacctc ccctgagtcc ggaaccgatg tcagatcatt ccacatcgta tcaggacttt
    17221 cgttaggcgt ccgtgtaccg aacttcgccg gtgatcaagg cgcgcttcac ctgctcaatc
    17281 gcttcggtga ccttaatgtc acgcgggcaa gcatccgtgc aattgaagac agttcggcat
    17341 cgccacacgc cggacccctt gttcaggatc tgcaaccgct cagcagtgcc ttgatccctg
    17401 ctgtcgaaga tgaacctgtg cgcgttcacg atggcagcag gaccgacgaa ctcgtcgttg
    17461 gtccaggtaa tcgggcaagc agtcgtgcat gccgcgcaga gaatgcactt tgtcgtatcg
    17521 tcgaaccggt ggcgctcagc aggtgactgt agacgttctc tcgctgtcgg agagtcctcg
    17581 gcgatgaggt atggctttac ggacttgtag gagtcgaaaa actggtccat atcgaccagg
    17641 agatccttct cgaccttgaa gccgattagg ggctcgagcg tgacccgctt cccaagatcc
    17701 tgcatgagga ccttgcatgc gagacggttg cggccattta tccgcatggc gtccgagcca
    17761 cagatcccgt gcgcacagga acgacgatag gcgagcgtgc cgtcatgata ccaccgtatg
    17821 tagttgagcg catccagtac ccggtcggtg ccttccacct ctaccttgta ttcctcgaaa
    17881 tgggccttgg tgtcggtttc agggttgaac cgcttgattc gaagggtgac ctccatggag
    17941 acgcctttct ctgctgatca aaatcgagat ccaaaatgag tgtggagcaa tctagtattt
    18001 gcgctctttc ggttgcatgg tcgtgatgac cacgggtttc ttttcggacc gcaaggagcc
    18061 gtcatcgttc cggtagacca tcgagtgggc cagccaattc tggtcatccc gagcctcgta
    18121 gtcatcccga tagtgagcac cacggctctc ttttcgggca agcgcaccct cgataatagt
    18181 ctccgcgcag tcgagcatgt tacccagttc cagcgcttca agcaggtcca tgttgtagat
    18241 gcgtccgcga tccatgatcg ctgcattggc atatgcgctc ttgagctcgg aaatcctcgc
    18301 ttgcgcctct tgcatgccga tttcgtttct gacgacggac accttgtcca tcatgacttc
    18361 ctgcatctcg gaacggatat cggcgatccg ctccccactc tgccggttca gcaacgtgtc
    18421 gatatctgag cgtgcattgt cctccgggtt cggagggagt tctgcccagt tgacgttttg
    18481 aatctcctcg tacatgcgtt ttccggcgcg acgaccgaag acgacgagat cgacaagtga
    18541 gttcgtcccc agccggttcg caccatggac agaaacgcat gccgattctc cggcggagta
    18601 gagaccagga acagcggttc ccgcggggtc ccggattacc tggccttcaa tgttcgtggg
    18661 caacccgccc atcgcatagt gagccgtggg ctggataggc acgccctcct ccttcggctc
    18721 gacgcccaga tagacgcgag cgaagtcggt aatgtcaggg agtttctcgt cgataacctc
    18781 gggcggcagg tgcgaaacgt cgagcaggac gtagtccttg ccatcgatgc cacgtccctc
    18841 cctgatttcc tggtgaatga agcgggacat catatcgcga ggggcaaggt ctttcatcgt
    18901 cggtgcatag cgagcggcaa atgcctcgcc ctcgctgttc cggacaacgc ctccctcgcc
    18961 gcgggccgct tccgacagga ggatgcccag cttgtaaagg cccgtcggat ggaactggta
    19021 catctccatg tccatcagag gaatgccatg gcggaacgga atggcgacgc cgtcaccagt
    19081 tcccgccatg gcgtttgacg tcaccttgaa gacacggcca aaaccaccac ttgcaataag
    19141 gaccgtcttt gcgtgaatag tatgtagttc gccagtcttg atttgatagg tcaccacacc
    19201 gcagcacgtg ccatcggcgg cgaagataac gtccaccagg tggtgctcgt cgaaaaaatt
    19261 gacgttgtgc ttgatcccct gctggtacag ggtctggatg atcatgtggc cggtgcggtc
    19321 cgccgcataa cacgcgcggc ggacgggccc ctctccgaag ttacggctat ggccaccaaa
    19381 ccggcgctgg tcgatccttc cgtcgggcgt ccggttgaag ggcaagccca tgtgctcgag
    19441 ctcgagaacg gcttcgatcg cttctcgggt gagaacctcg gcggcatcct ggtcaacgag
    19501 gtagtcgccc cccttgaccg tgtcgtacat gtgccattcc caatggtctt cttccgtgtt
    19561 cccaagggcg gcgctgatgc caccttgcgc cgcgccagta tgtgagcgag tcgggtagag
    19621 cttgctcacg acggcaatgt tggctttccc ggcgagctga atcgcagcca ttaaccccgc
    19681 gccgccagct ccgacgatga ccgcgtcgta ccggtgatat gccatcaagt tgctccttca
    19741 taccgggcag ctgcccgtac gcactctcac ctcttcattg tgccatttat gctgtgagtg
    19801 cgcgagataa tgccgctcgc gctgcctcaa ccggagccgc tgatctggtt ccggagaatc
    19861 gccagctcat cgtccttacg actcatggtg tcaggctgcg agcgagcgag attgaccaag
    19921 ataacgggaa atctccggta tacttccggg agcgcgatgc tggcgctatc cgccccaatt
    19981 tccacaggag aacgcacgtg ccatcgacgc agtcaccgcc gttaccggac gtcctgaacg
    20041 tcgccgaaac gtacgtagag cccatcgtcg agctcacgat tcgtctcagc gaagtgcctg
    20101 caccaacgaa cgacgaagcg ctccgcgcag ctgcggttcg ggaggaattg gagcaactcg
    20161 gatacgagga tgtgaccgaa gatgaactcc ataatgtcac tgggcgtatt gccgggatgg
    20221 attcctcgct ttgcctgttg ctcgccggcc acatcgatac ggtcttcccg agcaatgtga
    20281 acctgacagt gaccagggag ggtgatgttt tgcacggccc gggcgtcggt gacaatacgg
    20341 tcagtgtcgc ctcagtcgcc atggtgggcc gggctctgag ggatctcggc attgtgcctg
    20401 ccgtcgatat tgttgtcacc ggcaatgtgg gagaggaggg gctcggcgac ctccgcggga
    20461 tgcgtgcggt ggtcgacgcc cttcctaacc tcggtggtgc gattgcggtc gaggggcatt
    20521 cgctcggccg cattacccac cgggcggtcg gcagccgccg cctacgggtt accgtcaccg
    20581 gtcctggggg tcacagctgg ggacaggccg ggcttccgag tgcgatccat catttggcga
    20641 ggatcatagc tcgattggat gctattccct tgaccgacga tcccaagacc tccttcaatg
    20701 cgggcatttt tcagggcggc atcagcgtca acacgatcgc tccggaagcg gtagccgtcc
    20761 tcgatatgag atcgacgagc gcagaggcgt tggccggcct ggtttcagaa gtcgaacgtg
    20821 tgcttgcaac gccagcaccc gatgggatag atgtggacgt tgaggtggtc ggtaatcgcc
    20881 ctgccggcga gcttcctgcc gacagaggcc tggtcccagt cgctacatcc gttctccggg
    20941 agctgggatt cgatgtcgcc tacgatactt ccagcaccga cgccaacatt cccatcagta
    21001 ggggtatccc cgctatctgt atcggcctca ctaccggggg aaacgtgcac cgggtagacg
    21061 aatatatcaa tatctcccca ctggcaaagg gcttcgccca gttgctgtta acagcgaccc
    21121 gctctgccga tgccatagct accgggcagt tgtagcgcag gactcagaac ttcgtggacc
    21181 gctgagttgg ctcagcctgg aaagtaccgt tatcgtgccc gattctctta caggtgccgc
    21241 cgatgggctt gaatatgtgg ccatcgatgt cgaagcaaca gggttgaacc cacagaccga
    21301 cgaggttatt gaaatcggac tcgtggtctt cacgcgtgat ctcgaagttt cccggtactg
    21361 ccgcacgatt aggcccaaac agcaggtatc gcgcgacata cttcaactga caggtatcac
    21421 cgcggaggat ctggggacaa gcccatcctt cgccgaggtc gcggacgagc tccgtgagca
    21481 aatcgggtcg cgcccaattg tgggacagag tgtcgggcac gatctcgccc tgctcgcgag
    21541 tgctggactg aagcctgcga accggacgat cgatacgtac cggctggcgt cggccctgct
    21601 ccccgatctg ccgaattatc agttgggaac aattgctggc cacctggggc atgagatcag
    21661 cggggaggac aagcatcggg cgctacacga tgcactcgcg gcaatgcatg tgttcaaggc
    21721 tctcctggac aggatcgatg attacgatcc gtctaccctg agccaggttg caacgctcgc
    21781 gaggaccgca ggctggcccg aagctccatt tttccagaat gctgcacgag agcgacggga
    21841 agcacccttg ttcatggcgg atcgtcctcc gacggtgccg cttgagttgc agtttcttgt
    21901 ttcccgggac cgtccagaac ctctgcgccc gactggttcc tccgaaccag ttgatatcga
    21961 gcgccttgaa cgacttctag gcagggatgg tcctctctcg cgggtcctcg agaaatacga
    22021 acccaggccg actcaagtga cgatggccaa ggcggtcgca ggctcgctga acagcgaaca
    22081 gcagttactc gtcgaggccg ggactgggac aggcaagagc ctggcgtacc tgctgcccgc
    22141 cgccatgttt gccatttcgc gcggtgagcg ggtggtggtg agtactgcca cgatcgcact
    22201 tcaggatcag ctctatcgga aggaccttcc ggacgttcat accgcccttg tagaagcggg
    22261 gatcaatgaa gagctgcatg tcgcggtcat gaagggacgg cagaactatc tctgcctcaa
    22321 acagtggttc gcgcacagca atgaccccgt ggaggacgag catgacgcct ccctgcgcgc
    22381 caagatcctt ctttggctcg gccaaaccag taccggggat cgggcagagc tgcgtctaac
    22441 cacagaagaa gagcgtcact ggaggaagtt tgcgtcggaa cggggccggt gtaccgtggg
    22501 ccgctgccca tatgcaggca gcaaccagtg tttctttcac cgagctcgtt acaatgccct
    22561 gcacgcgcac atcgtgattg ccaatcattc gctggtcctc tccaacgcgg cggaaggacg
    22621 ggtcctcccc agcttcgagc ggcttgtcat cgacgaggcg catcacctcg aggatgaagc
    22681 aacaaggcag ctcagttttg tggtggatcg tcctgctgtc gacgatgcag tgaaggcgct
    22741 cgcacggtcg gacggtgcag ttcagggcgg ggcgattccc atcgccgccg cagtcctgac
    22801 caggttgaag gacagtactg caatcaaaca ttcggacaag gcccgatccc tggcggacga
    22861 agccgtgcac tccgccacgc agatcaactc gctgacgggc gagctgttca gtcgactgtc
    22921 acagttggta ggcaagccac gattcggtgg cggcgccggt tatgcccagt cgcagcgcat
    22981 tacagaacag atgcgagaca gcggctcgtt cgttcaggca gcactcatct gggaagagct
    23041 cgagcacctc ctgaggaaga tccaggaggc gggagcctgg ttcttgcagg tcatggacga
    23101 ggtgtccctt cccaatgacg acacgcaccc actggtccag cagcgggatg atgcggttgt
    23161 ggagctcatg acaggtgttg atcagctcag cgacgtaatg cgccagctgc acgagtgtct
    23221 cggcgagctc gaccccacca gggtgtattg ggtccagcgg tccggacagt tagggttgat
    23281 ctcgctgaac ggcgcgccgc tcgatgtcag cgttctcctg aaccagtatg tctatgcgag
    23341 tctccgcacc gttgtgttga cttctgcgac cttgactatc gacgggtcgt tctcgttcat
    23401 cgcagagcat ctcggcctgc tcgacgccac gacacttgat ctcggctcgc cattcgatca
    23461 cgaaaacact accctggtgt atgttcccga ggatatgcct gagccgaatc atcccaacta
    23521 cacgagcgtg cttcatgatg ttctcggcga ggcgctcatt ggaacgcaag gacgggcact
    23581 ggtcctgttc accagccata gagccttacg tgacgcgcgg aacgccctca aggaaccact
    23641 ggagagtcat aacatcattg tgctcggcca gggcgttgat ggaagttccc ggaatctcat
    23701 cgagcgcctg cggaccgagg aggggatggt tgtctttggc acgtcgtcgt tttgggaagg
    23761 cgtggacgtg gtcggagacg cccttagcat ggtgatcatc acgaagttcc cattctccgt
    23821 accgtccgat cccatcttcg aggctcggag cgagctgtac gatcagccgt ttatggaatt
    23881 gtcgctgccg cttgccgtgc tcaagttcaa gcagggattt gggaggttga tccggagtgc
    23941 ggaagatcgc ggcgtgtgcg ctatcctcga caagagagcc gtttcgaaac ggtacgggtc
    24001 atcattcatc cagtcactgc caccctgtca tgtcgtcgtc ggctcgtcgt acgaacttcc
    24061 tgacgaggcc gcaagatgga ttgcggcagc acccgctcgt gttggtcaat aacgcaagga
    24121 aatgtctcga tggacaaccc ggacatcagc agaaatattt cattagaact cgcgcgatgc
    24181 acggaagcgg cggcgctcat gtcggcccgc tggatgggtc gtggcgataa ggagacagcc
    24241 gatcaggccg ctgtcgacgc catgcgccag cttctcaata cgatcgacat ggacgccacg
    24301 gtcgtaatcg gcgaaggaga aaaggacgag gcgccgatgc tgtacgtagg cgagcggttg
    24361 ggttgtgctg atgcgccgag ggtcgatatc gcggtcgacc ccgtggaagg tacacgcttg
    24421 ctggcccaag ggcagccgaa cgcactttgt gttatcgctg tcgccgagcg aggtgcgttc
    24481 tacggttggc aagacatcgc gtacatggag aagatcgcgg tcgggccgtc cgcggcgggg
    24541 cggattgata tcaccaggtc agtaacggaa aacctgatca ggatcgccga tgccaaggga
    24601 atgaaggtgg aggatcttac ggtcgtgatg ctggatcgcc ccaggcacga agagatcata
    24661 cgggagacgc gcgcggcagg atcgcgaatc aagctgataa ccgatggtga tgtcgcggga
    24721 gccttgatga cctcgatgga ggggacaggc gtcgacctgc tcctggggat cggcggctca
    24781 cccgaggcag tgattacggc agccgccctt aagtgttcgg gaggcgatat gcagtgccgt
    24841 ctatggccac ggaatgagca tgagcgcgag ttggcccagc agaacggcat cgacctaacg
    24901 agggttttta cgattgatga tctcgttgga ggtgacgacg tcttcttcgc ggcaacgggc
    24961 attactgacg gcgaattgct tgatggcgtg aagtatgtac caggcggcgc aacaactcat
    25021 acgctggtga tgagggctgt gagcggcaca gtgcgctata ttcgttcgac acatttcact
    25081 cgtaagttgg aaaacctagc cgcggccacg aagcactagt cagtcactct gggctggcgt
    25141 gccgggatgc ggggaaactt gtggccattt acgttgacgc caaacggctt gagtggtatt
    25201 cttcgtgcat cgaatggcga gtctcgaaaa acccctgctc gtcatgtcac ctttctctac
    25261 tacagtctct tcaggtgaca cgttcgcttc ctccaaggtc tcaagctagg aactccatct
    25321 gcccacacgg cggagtggta tttgtggtga ggacctgccc aaccggcatg cgctgacgag
    25381 ttcacctgga tccacctggt tatgaaatgt gcgagccctc tcaaaggatg gtacgtgatg
    25441 cgcttggtgt gcgacattgc ctcccttgca accccctaga ttatctgtcc ctctttacaa
    25501 gttgcgttct gctggaccgg cgtatctgaa aacctttcgg aggtattcgc ttgactctcg
    25561 aagacaccct aacccaagag aagacgactg aggcagtcgc cgagaaaccg gtccgcagga
    25621 ctcgcactgt tcgcgctcgt cgcaaggtag acgaagacgc tgccggatcg aacggcacca
    25681 gcccggcgga cgttccggaa accgctcctg ccattgttca agaggctcct gtcgcacctg
    25741 aggtggtcgt ggcaactccg acagtacccg tcgtccagcc ggttgccgag acgccggctg
    25801 agcctcagga tggccagcaa cgtcagcgac gcaatgggaa ccagcggggc aacaagaagc
    25861 gcaacaataa gggtccggag cagaatggcc agcagggcgg ggatctgcag caggcgcgaa
    25921 ataatcggcc gcagcagaac cgcgagcggc agcaggagcc ggctggcgac cgtctgaccg
    25981 ttgccagcct cgatgcgcgt gcagatgatg ccttgcatga tcttgcccgt gagctgggcg
    26041 tttccgggta cctgcgtatg cgccgtcccg acctcatcac caagctgttg caagcccaga
    26101 cggaacggga cggacacatt ttcggcgacg gcgtgctgga gattattgaa gacggcttcg
    26161 gctttcttcg cggggaccgt ttcctgccag gtccagacga catctatatc tctcagtccc
    26221 agatccggcg ctttggtctt cgcacaggcg atcgcgtgag cggtcaggtc cgccctccca
    26281 aggacagcga gaagtttttc agcctgctgc gggttgaggc agtcaatggt attgaccccg
    26341 agactgcacg tcgccggccc gcctttgaca aattgacgcc gatttttccg ctggagttga
    26401 tcgatctcga gacagcgtcg aacgtgctct cgacgcgtct actcaacctt gttgcgccga
    26461 tcggacgtgg acagcgcggt cttatcgttt cgccgccgaa agccggcaag acactgctcc
    26521 tgaagtccat tgccaacggc atcacgagca acaatccaga catgcacctt atggtctgtc
    26581 tcattggtga acgaccagaa gaggtgacgg acatgcggcg ctccgtcgat ggagaggtca
    26641 tctcctcgac attcgacgag ccggttgagg accacaccaa ggtcgccgag atggctctcg
    26701 agcgcgccaa gcgcctggtg gagtcgggcc gtgatgtgac gatccttctg gactccatta
    26761 cacgtctcgc tcgtgcctac aacctcgcag ttccaccgag tggccggacg ctctcgggcg
    26821 gtattgatcc agttgctctc tacccgccga agcgattctt cggcgctgcc cggaatatcg
    26881 agggcggcgg ctctttgacg attgttgcca cctgtctcgt tgacactggc agccgcatgg
    26941 acgacgtgat ctacgaggag ttcaagggca ccggtaacat ggagcttcat ttggaccgga
    27001 agcttgccga gcgccgtatc tacccggcga tcgacatcca acgctccggt actcgccgcg
    27061 aggagttgct ccttagcgat tcggtgctcc ggcaggtatg gacgatgcgg aggatggttt
    27121 cggtgctggg tggcaccgaa ggtaccgaag ccttgcttgg caggctgtcc aagacccagt
    27181 ccaatgccga attcctcgag ggcctcacta gggcagacta acaaggcgtt gtcagttgtg
    27241 cagccaccaa atgcccttcc tgtgacacca caggaagggc atttgcgcta tgtgcgacag
    27301 aagcggctac tgaacatcga ccccaaccgt ttgcaggttg cggtgagacc ttgctgttgc
    27361 gcctggtcag cggaccatag agatatcgac gtgcgttcgt ggtcgctcgt ctggctccga
    27421 catttcttcg gtcatgacga cataggtctt gtccggattt gtcgatgctg cgcctggatt
    27481 gccgtcccgg tccagcgcga ctatgttcat ctcgctggca tacggatcgt ccaaatgttc
    27541 gagatccttc atagcgtggt gcagcgcttc ctggagcgac atgccgaaac gcataaaggt
    27601 gacgacactg tgcgctgtga cgcaccgttg cgccatctcg ccccgcccgg tgcaggcggc
    27661 ggcaccccag cggttatctg cgtaattgcc ggcgccgatg atgggcgaat cacccaagcg
    27721 gcccggatat ttccatgccc aaccagaggt gctaacgcca caggcgatgg ttccatgacg
    27781 gtcacgagcg atgaaattga ccgttccatg agccggctca cccagttgct cggcgatttc
    27841 agggtcttcc gcggttttcg ccacgagttc ctgaatacga ccgaggtatg aggcgtctcg
    27901 atccgtctgg tcctccaggc gagacctcca gatttcttga gcttctggcg taaggagatt
    27961 cttcggctct aatcccattt cgcgagcgag acgtgctgcg ccctcaccgc cgataaggac
    28021 gtgcggcagc gtgtccatca cgtagcgagc caggtcgaca gcatcctggt acccctgaag
    28081 tgcgccgacg gaacccgccc ggaggccgga tccctccata atgctcgcgt ccagctcgac
    28141 atcgccaaga aggttcggaa gacccgaata ccccaccgta tgatcgtcgg ggttggcctc
    28201 gacgaggcgg atgcccgcga ccacggcatc cagagccgat ccaccgctgc gcaaaacgtc
    28261 gacagatgcg tcaataccga tgcgcccatt gtgagacgca accacgattc cgctcattcc
    28321 gtgtcatcca tctcgtaacc agcagccaac aatgcggcgt ccagctgcgc tgttgcaggt
    28381 tcgacgagca ccttgtcgcc tatcaccaga gtcggtacct tggcctggcc ggttagctcc
    28441 agcacctcct gcctgcgctt cgactcgcac tcgatgtcgt attcaatcca ctcgtgcccc
    28501 agttcctcca gacggagctt ggcgcgggcg acatcgggac agaaggtttg acgaccatag
    28561 agaatgatcg gaggacggtt gtcagacatg tagtactcct ggactcgcag tgtaccgtta
    28621 gagaacacgg gttatttcat cagaatcgta gcggcgaagt ggcaccgttt catctcccac
    28681 gatgagttga acgccatgcc tgatatcggt gagcctgccg attcgggtaa ccggaatgct
    28741 tgcagcctcc agcgtctccc ggagcttctc accagttccc ggtttcgcag ctatgagcaa
    28801 ggctcctgaa ctcaatagac ctagtgggtc gatgcttagc tggtcgcaga ttagtcgggt
    28861 ctcctctcgc acagggatcg aatcatgttc gacaagagcc ccgagggacg atgcttcggc
    28921 cagctcgtgg atcgccgtgg caatacctcc ctcggtcgga tcgtgcaggg cagttaccac
    28981 tccggacgcc aacgaagcaa gcgcgtcagg gaccaccgac acaccggggt ccctaagcaa
    29041 cagtttggcc ctttcgatga cgttctcacc cagcgcggca aggtccgtat cacaatcatg
    29101 gccgagcaag gccgtgccct caataccgat ccattttgta acgtacagct catcgcctgg
    29161 agatgcttca cccgggcgga ggagaccggt tggcccgggt acgccgagca ttgtccctac
    29221 gagcaacggg cggtcaacgc ccagagtaat ttcggtgtga ccgcctatca gggcaacgcc
    29281 tgcttcgaca catgcttccc gaagttcaga aaaaaggcgc tccacggttg ccggggtggt
    29341 ctttccctca ggtaggagca cgacgaccgt caaccagcga ggaatgccac ccatgcatgc
    29401 aatgtcattt gcattcacgg cgacgaggta gtggcctgcc ccgtcagtag caaaggtgat
    29461 ggggtcgctc ttgaccagta gggtctctgc cccaagcaat acggctgcgg cgtcgaaccc
    29521 gtacttgggc tgcacgatga cagaagcgtc ttcgggcgtt cggtactgtg atatgagccg
    29581 ggccaagagc tccccaggga gcttccctgg gtccaggggc tgggatgtcg cgtctcgcat
    29641 cttattgatc ggcttcatcc tcacgtgcaa gttctgtcac acgcagttcg gcgtccgcga
    29701 taagtgatcg gcacagatca atgagatgcg cgccagcctt atagttggca atcgactcct
    29761 ccagcgtgag atttccgctt tcaagggccg aaacgatttc ccggatcttc tgcaaggcat
    29821 cttcaaaaga tatttgttcc tcagccctgc catccctggt gtcgatcatt ctgatacatc
    29881 ccttcctgcc agtgggctgg agtgccgcct gacgtccgtc acctgtgcgg tcaattctcc
    29941 gtcggcaagc acggcccgga cggaagtccc cggccaagcc tgactgactt ccctcactgg
    30001 agaacccgtg tccacatcac agacaaagct gaagccgcgg cacatgagcg cgtgagggtt
    30061 gagcgcctca agaacctcgt gaaaggcatc agtatcgtga cgcgcgaact ggagtctttg
    30121 acgtattgcg aggtggagcc gatcttccag cggtgccaga tccctccgaa gcgaggtgac
    30181 tgcggccgtt ggagagacgc gggcgaggcg agcctgcatg tcgtccaaaa cctgaagcga
    30241 ctgctccagg cggtcttgta tctcgacacg aatgcgcacc tgggcctcat tgagagcgtc
    30301 atcgatggcg ctgagatctg gcacggccat ctcggcggcg gcagaaggag tgggcgcccg
    30361 tacatcggct gcgtagtcac tgagtgtcat atcggtttgg tgacccaccg cagagacgat
    30421 cggaacacgg gacgcaaata tagcgcgaac aaccctttca tcgttgaacg cccaaagatc
    30481 ttcgagagat ccacctcctc gtccaatgat gatgacatcg ggttcagcaa cctcctgaag
    30541 gcagaccagt gccttcacga gcgaatccgg cgccgtctcc ccctgaacgc ttgcggggga
    30601 aagtataagg tgggccagtg ggaagcgtcg gccgacgacc tgctggatgt cgtgccagac
    30661 cgcccctgtg gctgaagtaa cgacgcctat gcgtcctgga aagaggggca ggggacgctt
    30721 gcgcgagggt tcgaatatgc cctcggcttc gagcttctgc cgaagctcct caagcctcag
    30781 ttgcagcaat cccgtccctt ggggctggag caaatcaacc tgtaattgaa gattcccccg
    30841 cggttgataa aacgtcatgc tgccatgcgc aatgacgcgg tcgccctccc gcggaatcat
    30901 cttttgccga gcaagcgcgg tccgccacat gaccgcgctc agctgactgg tttcatctct
    30961 cagggtgaag tacgcatggc cagaacgcgc aaccgagaga ttggaaacct cgccgtcgac
    31021 ccagaggtcc tgaagatcgt catcgtcttc gacaagcact ttgaggagag aggtgagctg
    31081 gtcaattgag actatccgga gcatcaccca agcgagtccg tctgcgatac gcgcatcagg
    31141 ggagagccgt actcaaccgg ttgcgcgttc tccacgagga cctcgacgag aacgcccgaa
    31201 taatccgcaa caatctcatt catgatcttc attgcttcga cgatgccaat aacctgacca
    31261 acctcaattt cgtcgcccag ctggacgaac ggcgcctctc caggggcggg ggctgaatag
    31321 taagtcccaa tcattggcgc ggttataaga tggccctcgg gatcgctttg cggtaatgca
    31381 gccatcaccg attccgtgct ggttactccc tgggtcgcga cgagggtgga cgaggcacct
    31441 ctcaggcgaa tggtcacatc accggtggaa aggtcaagct cgctgatgcc gcccttggac
    31501 atcattgaaa tcaactcccg tacggcttcg atcactccgt ctatcgtaaa tgacggaggg
    31561 gtgtttcgat cgtcttgacc ggtcatgcca tacacctgtg tcgtgggcaa agagcgtggt
    31621 cacgccatgc ctctgttgcg ggcagccgtc tgccgcgccg gaagttggta gagaaggcga
    31681 gtcttgattc ccgcacctca ttcttcagat gacgcgctcg atgtattccc cggtgcgcgt
    31741 gtcgaccttg agttgatcgc cctcgttgat aaagagcggg acgttcaccg ttaacccggt
    31801 ctccaatgtt gccggcttat tcccgccggt cgccgtgtcc cccttgaagc ccgggtctgt
    31861 ctgtgtcacg gcaaggacaa cggaagttgg aagttctacg tcaattggct cgccattata
    31921 ggtgaggaga tcgatcacgt cgttctcctt cataaatttt tcggcatcgc caactgcctt
    31981 gcgatcaagc gctatctgct cgaatgtctc tgtgtccata aagtggaact ggtcgccttc
    32041 agcgtagagg aattgcacgt gttggcgttc caggcggaca agttggaact tcgttcctgc
    32101 cataacggtc atctgcgtat ttgagcctgt cctcaggtca cgcagggtca ttcgcagctg
    32161 cgccgaaccc cggccctgct tgttgtgcga gaactcgatt accttaacga gcttgccatc
    32221 tatatccagt gttacaccct tacgaacatc gcctgtatcg atcatgcatt cttctcctgc
    32281 gttgcttcga cggaacgctt gggcgaactc gtcaagtttt gggcgccgtc ctcggtaatg
    32341 agtacaacgt cttcaatgcg aacaccgccc cactctggaa tgtaaaggcc aggctcgatt
    32401 gtcacgacgt ggccaggctc gagtgtatcc cgggaacgca tgctcgcaga cggagcctcg
    32461 tgaactcgca agccaagccc gtgccccagg gaatggacga aataatgatc caggccctgc
    32521 gctgtaaaga cgtcacgcgc agcagaatcc agctcgattc ctgttacccc gggagccgcc
    32581 ttggccaacg ccgcgtcgtg tgcggcctgg acggtccggt acatggtaac aagttgcccg
    32641 gatggctgcc caaaccagat tgttctcgtc aagtcccccg cgtaaccaaa gtgaagggcg
    32701 cccaaatcaa tgatgatcgg ctctccctcc tcgataatcc ggtcgcccgg agcgtgatgc
    32761 ggcttcgcgg cgttgggtcc ggacgccaca atcgtcggaa acgcctcgcc atcactgccg
    32821 gcacgtcgca gttcctcggc gacgatttct gctgcctgtt tttccgtcat gccggcctgt
    32881 atacgcgttt ccgcggacat aaacgcctga tcggtcaacc tgagcgcatt ggtcagtagc
    32941 gttatctcag actcgtcctt cactgcgcgt aggctgtctg tggcgtcgcc gaccggaacc
    33001 agatcgactt ggccaccgag agcctggtgt agctcgttgt acacggcgac agtggtagtc
    33061 gcgtcctcga atccgaactt cgtccaaccg gacgttttga ccagctcagc aatggagtcg
    33121 gtccactttc tctccgcagg gcggaccatt actccaggga gtgcttcaga ttctgcccac
    33181 gggagattgg ttgagggagc gaacagcata acttcgtccg gggaaacaac agcagtcgcc
    33241 gagagttcgt caggtgcgtg atcattagct gtaaaaccgg tgagataccg gcggttggtg
    33301 ccattcgtaa tgagtaccgc atcaaggtca agcgtgttaa gcagagcccc ggcacgtgcg
    33361 aggcggtcgt tcgtcatctg aaactcttct cctcaatacg ttttgcttgt cgtcgaaacc
    33421 attccagagc gagatggtag ccgtcctccc caagaccgac aatctgtccg gttaccaccg
    33481 cagagataac cgagcgatga cggaaatcct cacgagcatg cacattggag atatgtacct
    33541 cgatcgccgg ggtctcgatg gaggtaagcg cgtcccggag cgcaatgctg tagtgagtga
    33601 gggcaccagc attgacgatt attccgaatg catcaggtcc ttgatcgtgg atcgcgtcga
    33661 tcaggacacc ctcgtggttg gattggtagt ggaggaggct cagagcgggc gtcccagagg
    33721 cagcggcacg ctgcagattg tccatcattt ccgcaagcgt caggcgaccg tagatttcgg
    33781 gctgacgcgt tcccagcatg ttgaggttgg ggccgttgag aacgagaatc gtggaggcgg
    33841 cactgcttgc aagcatcctc tatcctaccg gctgctggct gttgatcagc gccgggacat
    33901 acgaggtgat gggataactc gtatccgacc cttccggagt aacctggccg ctgaagacat
    33961 agacgggctg cagatattgc tgctcgcctg gaatgccgga ggatgtgtat gcgagggaga
    34021 cctggtcata cgtcgccaca ccggtgatca tggctcccgg ctcgtattga tctgatggca
    34081 ggagtgtttc caggtaggat cgctggcttt cgacttccgt ccatgcagcg ctcgtgcccc
    34141 ggagctggta gaggtcactc tgggagatat ccgcccaccg gtatgaagac tccaggaccg
    34201 caccctcggg accgagtgtc actgtgatat ttggagtggc cgagagcagg ggcgaagggg
    34261 tgatcggctg gaagctgacg atgatacgtg gcgggtcttc gattcttgtg acgaccttgc
    34321 cctcgccaac atccgcaggc agaaggttca cctgcctcag ccactcccgg gcaaatgcga
    34381 taccctgctc gtccgtcggc aggtcgccct gcggaatctc cgcgaacgag atgaactgca
    34441 tcatgcctgg gactatgaag aggctcccgc tctcatcctg aacgtcaaat gtggcgttgt
    34501 cttcggtttc cacctcgccg tcgataccga ggttccccgc caacgtcttt gcccgctcgg
    34561 cgtccacttg cgggaaacgc atggtgtaaa cgggcgcttc ttcgggaagt tgggtgaagg
    34621 ctccgtcgta tgtggattcc aattcgaaga caaaaccctg ctgattcatg gtaggcaggt
    34681 cagcggccgg aagctgggag gtcgtctcgg aactgatttc agacccgggg accggtgttg
    34741 agccgtcctg ggcaaggacg ggcgctgctg tgctgagggt gagcgtggca ctcaggataa
    34801 gcattgatag gaacgatgat cggcgcgttg aggtgaatgg catatggcgt gtcctttctg
    34861 ggtagcgtgc ctcgcgggat cccgtgtggc ctaaaacgcc ttcacagggt cgacctcgac
    34921 gatgcgtctg agctccacga tctgatcgcg aagtaacgcg gccttctcga actcaagatc
    34981 gcgagcggcc gtcttcattt gggactcgag atccttgatc attcgcgcga gctcatcgcg
    35041 gggaatctgt gagatgacac cggagccctt tgcgccgtca gtactgtact ccgcgtgctc
    35101 ctcggcgacc gcacggacgc gttccgtcag gtccttgatc tgcttcacga taccccgggg
    35161 ctcgatgccg ttcttcgtgt tataggctat ctggatctcg cgccgccggt aggtttcgtc
    35221 gatggctgcc ttcatcgacc gggtcatggt atccgcatac atgattacct ggccatcaac
    35281 gtggcgggca gcacgaccaa ttgtctgtac caatgaaccc tctgatcgca ggaagccctc
    35341 tttgtccgcg tccagaatgg ccacgaggga gacctccggc aagtcaagac cttcccgcag
    35401 gagattgata ccgaccacca cgtcgtgaat gcccaggcga aggtcccgca ggatctcgat
    35461 ccgctcgaat gtatcgattt cgctatgcaa gtaatgagtg cgaacgccca tctccttgag
    35521 gtagtccgcg aggtcttccg ccattcgctt ggtaagcgtc gtaacaagcg ctcgttctcc
    35581 cttggcgaca cgggcattga cctcggcaag cagatcgtcg atctgcccct tggtctggcg
    35641 gaccgagacg gcaggatcca gcagaccggt aggacgaatc acctgctcaa cgatgcggga
    35701 cgaatgctcg tactcgtacg gccccggcgt cgcggatacg aagactgcct gatggatgat
    35761 cttctcgaac tcggggaacg tgagcggccg gttttccttg gcggagggaa gtcggaagcc
    35821 atactcgaca agtacctcct tccgcgatct atccccgccg aacataccgc ggacctgtgg
    35881 cagggtaatg tgagattcgt cgacgatcat caggaagtcg ctcgggaagt agtcgagcag
    35941 ggtggagggc ttgtccccga actggcgccg tgagagatgc atcgagtagt tctcgatacc
    36001 ggcacaatag ccagcctcct gcatcatctc gatgtcgtac atcgttcgct gcttcagccg
    36061 ggctgcttcc aggaccttcc cctgcgactc cagttccttg agccggtcct gcagctcttt
    36121 ttcaatgtcc ttgatcgcga cattcatcat gtcgtttgtg gtgacaaagt gcttcgcggg
    36181 gtagatcgcg atatcctcgc gttcggtgag cagttctccg gtgaggggat cgacttcaac
    36241 gatgcgctca acctcatccc cgaagaattc gactcgcacc gcgatctctt cgtacgccgg
    36301 gtatatctcg agggtatcgc cgcgcgcgcg gaaggagccg cgaatgagcg tcatgtcgtt
    36361 gcggtcgtac tggatgtcga caagttgacg cagcaccttg tcgcggcgga tctgctgccc
    36421 ccgtcgaagc gagatcatcg tcttggcgta ttcctccggt gttcccagtc cgaagataca
    36481 ggagacggac gcaacgataa gggtgtcatc gcgagtaagc agcgcgcggg tggcagcatg
    36541 gcgcagcttg tcgatttcct cgttgatgtc tgcatccttc tcaatgaagg tgtccgtgcg
    36601 ggcaacgtag gcttcgggct ggtaatagtc gtagtaggaa acgaagtact cgaccgcatt
    36661 atgcgggaag aactccttga actcggaata gagctgggca gcaagggtct tgttatgggc
    36721 cagtacgaga gtcgggcggt ttactcgctc gaccacattg gccatggtga aggtcttccc
    36781 cgagcccgtg acaccgagga gggtctggaa gcgttcgcca gctttcaatc cctcggtgag
    36841 ctctgcgatt gcggttggtt gatctcccgt aggtcggagg tcagaaacga tcttgaagtc
    36901 tggcatgcgg tctccggaca acctttaata cgatcaagtg ttcgagtata cggcgtatga
    36961 cccactcgtg gaagatcagg tgccctcttc ttctttcgat gcggaatcgc cttggtggct
    37021 ttcgggcggc tgatacttcg cctccgaaac agaggcaacc gtggccagcg acgctccgtc
    37081 aaactcctca gtcacctggt catcgtcgtc cagatcgtcg cgagacccgg ccgcctgctg
    37141 gctcggctgg atcgtggcca tatcaatctg atagatacct gtctcggcct caccaaggct
    37201 catgatctca tcgatgtggc ggacagccca tctctggccg gagaagggga gacgcgcggt
    37261 agcttcatcg cgaccgaacc aggcagcgtc gcgatagtca tccccaagat cgataggcga
    37321 ctgcgggctc acattgacgg cgaagaccgg ggcaagcacg atcacatcgc gcccctcgtc
    37381 gtaaaactgg ttgatgtagt ccgcggagta gacggcacta acgttgagac ctgccaaact
    37441 cttcacagtc tccgtgatcg tctgcagtgt ggtatctccg acgcgtactt gagaatgaaa
    37501 cgcctgccag gtgtgcggaa gagcaacgtc agaccggcgc tggagcagca ggaattggac
    37561 acgggcattg aggcggcgga agacgtacac atcaacgata tcggagagta cctgaggcat
    37621 gcagcggatg ctccatctgt tgggcggttc gtgtatcggg atccctgaac ctgacgcgcc
    37681 tatggacgtg ggcggcgatc acggagccac tgccggatct gctccagcgg cgtagggtac
    37741 tccctccggt tcgccaggtc gctcatgtct cgtccacgcc acatagtggc cgtaggcgtt
    37801 ccgctctgct tgttccggat gctttcgaga atcggccaga aaaacgcaat gacggcggcc
    37861 gatacggcga ggtgcgcaat gaacggggaa acctcgttca ccacccgggc cacgattgcg
    37921 agtaagaatg ccacgacaaa tggcgacacg gtgagccaac gatgcgcgga tgaaccaata
    37981 ttctggacgc gagggcttgt cagcgggtta ggcgtgcgct tgcgcttttc gtgctcgaac
    38041 gaaatcggtg gtggcagcgg gttgtcccgc tccgcccgct caagaatctc ttcgatctgt
    38101 cgttcgagcc gggttttcgg tcggtgccct tctggcttct cagggggatc ggtggttgct
    38161 ggcatggtct ctacaccagt gcagacaaga aaaagctgag gggtgcccca tggcgcctgt
    38221 ctcccaatgg taccctaccc ggtgacaagc gaggtgccat agggtcagag gaatcgaggg
    38281 aaatgagtag ccagtcagca gtgcagagtc gcagtcaatt ccgctgcaac gtcatcgatc
    38341 acaaggggac cgtgagtttc gtggcaccgc cacacggttt gaaagttatt gcagcgtcga
    38401 tcgccagtgg ggcaagcagt tttgcagaat tgatagcgca tgcccggcga ttcgatagcg
    38461 cctgggcgga gtctgtcaac agggatctga acaggttcga cgagcataac gtggaaacag
    38521 ttgggctatg gtttgacacc gccctgagtg cggaagagga cacccgtcgt cagtcgttcc
    38581 gggtgatcga ccagacaacc agacggcaga gcctggtgcc aggtgggctt gggctggtcg
    38641 tgttcaatct caaggagcaa cggatcattc aagtccagaa cagctacgaa gatctcaggc
    38701 ggaaggatcg ggggcgggtt cgcatcaacg ggaagccgac aaggcgcctc tttcaatacg
    38761 agttgccgtc gggatgggcc atcgtaccgt aatcgattca accgtgcctg gaccccgcca
    38821 tccgattttc ctggcggcta ctccgctggg gccggtatct actgtgttat ccggaaaacg
    38881 ccggaaatca ctttacagtc ccgatacgcc gagacgcacc gatgggctta accggtccac
    38941 ttcttgccct tgtttttgtc gcgtcgccca agccccccga ggaacatcct acccattgag
    39001 tcggaaactt tctcactcgt aaggttgcct gcgctctccg gattccgttc tgatgctcgg
    39061 ctttggacca cttcagtccg cgctcgctgt tcctgcgctt cagaaaagaa tttgtagatc
    39121 tgttctcccg cagctttctc atcctcgagg gccaggacgg cccgaaaatc ctccagcgtc
    39181 acgatcatct gattgatcca gcgcgagatt gctgccctgt tcgtcaggga gatatcgcgg
    39241 tgcatctcag gactccccag agcgagacgg gttgtgtcct tgaatccacc ggcggcaagg
    39301 gtgcgcatgt cacgccatga ttggtcggca gtagcagtcc gaacgagcgc cgcggcgacc
    39361 accatcggca agtggctgac gcctgccacg taactgtcgt gttccgctgg atcagcaaag
    39421 aaggcctccg cttcgacggc cgcgacgagg ccgagaacat tcttgatcgc ggcctcactg
    39481 gcggtgacac cggggcagac gacccaggtg gcattcctga agagatcggc gtctgctgct
    39541 tccaggctct cggacttgcc agccatgggg tgtccgccca caaaggaaac gtgtgcaggg
    39601 agttgtgccg cccattccag tacgttgacc ttcgtcgaac cggtgtcggt cacgatcgcg
    39661 tcttgcttga ggtgtggcgc gatgtcggag aaaatctcac gcattgaccc aacgggcgtc
    39721 gcgatgatga ggacatcggc gggtcgtacc gcatcggcaa gcgaccacgt tgtgtcgtcg
    39781 actgcaccca ttttcttggc gagtgtctgc ttgtccatgt cagagtcgta gccaatgaca
    39841 cggagggcat tctcatgctg cgcggaccag cgccggaggc ccaatccaat ggatccacca
    39901 atgaggccca gaccgagaat cgagatttgc tgcattgctg aagtctccta gggaacgtac
    39961 tccgtgacgt caagttctcg aacccagcct tcctccccgt cctcggtacg gaagtgcatc
    40021 cagacctggc cttcggcaag cccatctcgc tccgggttat cggattcttg ggactcgccg
    40081 aggtaaagca gtggttcttc gagggggagc gccctgattg ccggtgtgtc ggtccccgga
    40141 ccggatcgca gattgacact tccaacgctg tcgatctggt aatccgggtc aaattcgtcc
    40201 tcgtcgggtt cctcggtggt ctccgcctca gtagtttcgg ccggtgcttc ggttggtgca
    40261 ttggtcgggg ccggggcagt ggtcggttcg ctcgatggcg gctgggtcgg ggcagccgct
    40321 gctaccacag cgggttctgt cgcacgtgcc tgattcggtt cgttccgggt tacaaagtcg
    40381 ccgacaatag tgtccaactg cgcatagatg atggaaaagc tgccaacgca gacggcaatg
    40441 gtgagcagca tggagatgcc aatcatgatg cctgcacggc gcgatcgctg ccggagacga
    40501 tgattcagtc cgggatcggt catgggtcct ttctcaagga gtggtgggaa ggttggcccg
    40561 gtatggacta gcgggcaacg tgtatagagg tgacatgctc gcggtcagac cgcggtacaa
    40621 ccgctcagct cgttcgcgga atctcctagt tatagcacgc acaggactgc cggaactctt
    40681 ggcaaattga accaaaccca ctaaacatgg tacatttatc tcggaaccga acctggtggg
    40741 atcaaacgtg ggcccacgcc cgcgtttctt gttgttcggc agattagctc cagcctcccc
    40801 acccccgtcc ctttctttga ttgattctat agagcaggag gtgcgcgatg aagagtgatc
    40861 tctacaccgc gatcgcccag attgcagccg agcgcggcat cccccgagaa gccgtcctga
    40921 gttccgttca gcaggcttta actgcggtgt acaggaaaaa tgcgaactcc gaggaggatg
    40981 ttcgtgttga gctagaccag gccaccggcg agatgcaggt cgtggtggtg aagaccgtag
    41041 tggaacaggt gaccgatccg gatctggaga tcagcgcgga tgacgcccgg gccttcgcgc
    41101 ccgcgcccat tctcggcgac caaatcaaga tcgttcgaac cccggagaac ttcgggcgga
    41161 tcgctgcaca gacggtcaag caagttgtgc atacacgaat tcgcgacttc gagcgggaga
    41221 ccgtctacaa ggaataccag gataaggaag gcgaagtcct cagcggtgtt gtgcagcgcg
    41281 ccgacaaccg cgcggtcatc atcgaacttg gcaaggccga ggccgtcatg ccggctcgcg
    41341 agcaggtccc gacggagcga tatcgcccca atcaacggct aaaggtgctg ttgacagagg
    41401 tgaacgccca tgctcgtggt ccccagctca ttgtttcccg ttcagatgcc cggctcattc
    41461 gccggctgtt cgagcaggaa gtgcccgaga ttcagagtgg cgctgttgag attatggaga
    41521 tcgcacgaga gccgggtctc cgctcgaagg tagcagttgc tgcccgacag gagaaggt
//
LOCUS       DASYWM010000070         4546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_227643, whole genome shotgun sequence.
ACCESSION   DASYWM010000070 DASYWM010000000
VERSION     DASYWM010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227643"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VGR29_02820"
     CDS             complement(<1..437)
                     /locus_tag="VGR29_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV2072551.1"
                     /translation="MKIVRFSTSHGGDAQYGVLEGTTVYQAEGDLFAGLTKGSEVGSL
                     DDVTLHAPIEPGKVIAIGLNYVDHVTELDKDRQLPTTPVVFMKPQSAIVGPDEAIEIA
                     HPDHDTHYEAEIVVVIGKKAKDVSEGEALDYVLGYTAGNDVSDR"
     gene            581..1354
                     /locus_tag="VGR29_02825"
     CDS             581..1354
                     /locus_tag="VGR29_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072552.1"
                     /translation="MPELNIQLMDPESGEVLPQPELPEDEALKLLERADITGAELVPW
                     GSNYTFAVALEAEGVPGHLGIYKPRTGERPLHDFRSGSLYARERASYLLSRALGWDIV
                     PPTVVRNGPHGIGSVQIYVPAQPDVAEDYEFWGARTLENERIVLFDHLANNADRKITH
                     CLLSTSGKVVGIDHGLTFNEVPKLRTVLWQFVGRPIRESLRKDVQKLVAGSQDLTDQL
                     AQLLTPQEIAAFWARTHAILETGKYPRLDPNRNVPYGWW"
     gene            1639..2643
                     /locus_tag="VGR29_02830"
     CDS             1639..2643
                     /locus_tag="VGR29_02830"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEV2072553.1"
                     /translation="MRWNEIFSSVDAHVAGQALRLITFGVPKLGDAPIRKQYEEMQGK
                     YDHIRSWLLSEPRGYSGMTGAVLLPSHDPKIDYGVIFMSSAGYSPISGHGLIALSTAL
                     IETGAVPIDGPDTRITFDTFVGPVQARASVDQGRVRGVRFRNVPAFRLVHDLPIEING
                     QTVPVDIAYGGNWYAIVKADDLGVSLEASQAAELAQRGMAVMRASERATDVVHPQDPD
                     LSGLYGVIILGEPKSEDSTSRNATVYLGGLIDRSPCATGMAATMACLAADERLAVGAS
                     FISESIVDTTMSGRIVVGTAVGEHQGIVVEISGRGALTGMHQFFVDPAEWRSSGFVQL
                     "
     gene            2839..3141
                     /locus_tag="VGR29_02835"
     CDS             2839..3141
                     /locus_tag="VGR29_02835"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEV2072554.1"
                     /translation="MGDNKLPDFREHDRDIQAVGTAAGLGFSLVASLVFFIAGGAWLD
                     SWLDTAPVLILVGVALGLIAAGYQLYELTLLGKPDRENGPIGRALERRASAKSRRR"
     gene            3290..4252
                     /gene="atpB"
                     /locus_tag="VGR29_02840"
     CDS             3290..4252
                     /gene="atpB"
                     /locus_tag="VGR29_02840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV2072555.1"
                     /translation="MELHISLAAEALFHLGPIAVTNSMLVMFLVMAAILLVGSWLARK
                     AQRDPHPGRAVGLAEMLVEFLLNLVESTAGKRIGRRIFPLIAGIFIFVLFANFTGLLP
                     GVGTIYIKEDAEEVVSGEEATLETTEEHSEEEQVAGEAVAADEHAAEEEHHPVNVPLL
                     RPPTADLNMTLAMSVLTFTVVQIAGVSAHGVRGRIKHMANPPFLFPIEVISELSRIIS
                     LSARLFGNVFAGEVLLGVMYAIAASIKIAVIPVLAPVVFLGLETLFGTIQALVFALLT
                     LIYVFLAAAGHDDHDEQHVDEHDTVRPVEAQAHSSGSMPASAGD"
     gene            4347..>4546
                     /locus_tag="VGR29_02845"
     CDS             4347..>4546
                     /locus_tag="VGR29_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEV2072556.1"
                     /translation="MGPPAGSRGTDRRKHHVFGGITDPEAVRYIGSGIAIGIGGLGPA
                     LGIGMAVRGAMEAIGRNPEAAG"
BASE COUNT          886 a         1247 c         1415 g          998 t
ORIGIN      
        1 cggtcggaga catcgttccc tgcggtgtaa ccgagcacat agtccagcgc ctccccttcg
       61 gagacatctt tcgccttctt gccgatgacc accacgatct ctgcctcgta atgcgtatcg
      121 tgatcgggat gcgcgatctc aatcgcctca tccgggccga caatggcgga ttggggcttc
      181 atgaacacaa ccggggtcgt cggcagctga cggtccttgt ccagctcggt cacgtgatcg
      241 acgtagttga gaccgatcgc gatgaccttg cccggttcaa tcggtgcgtg aagcgtcacg
      301 tcatcaagcg acccaacctc ggaccctttc gtaagaccgg cgaagaggtc gccctcggcc
      361 tggtagaccg tggtgccctc aagaacaccg tattgcgcgt ctccaccatg actggtggaa
      421 aatcggacga tcttcatgat gatgcatcct ccttgtataa cgaggttttc gtacaccgcg
      481 attgtattgg ggaatgactt tttgcgcgag agcctagtaa tgggcgattc tccatcctac
      541 tatgggtatc caaatcgtga cctttggaac ggggaacagg atgccggagc tcaacattca
      601 actcatggat cccgaatcag gtgaggtcct gccccagcca gagttgccgg aggacgaggc
      661 gctgaagctg ctggaacggg cggacattac cggagcggag ctggttccct ggggaagtaa
      721 ctacaccttc gcggtagcgc ttgaggcgga gggagtacca ggacatctag gcatctacaa
      781 acccaggaca ggtgagcgcc cgctgcatga ttttcggagc gggtcgctgt atgcccgtga
      841 acgtgcgtcg tatctgttga gccgggccct cggctgggat atcgtgccac cgacggtggt
      901 gcggaacggc ccgcatggaa ttggcagcgt ccagatctat gttcccgccc agccggacgt
      961 tgcggaggac tacgagttct ggggcgcgag aacgcttgag aacgaacgga ttgtgctctt
     1021 cgatcatctg gcaaacaacg ctgacaggaa gatcacccat tgtctgttga gcacgtccgg
     1081 caaggtcgtc ggtattgatc atggcctgac cttcaacgaa gtgccgaagc tccggacagt
     1141 gctttggcaa ttcgtcggtc gtccgatccg ggagagtctg cggaaggatg tccagaagtt
     1201 ggtcgccggt tcgcaagacc tgacggatca gttggcccag ctcctgacgc ctcaggagat
     1261 tgcggcattt tgggcccgca cgcacgcaat cctggagaca gggaaatatc caaggctcga
     1321 tccaaaccga aacgttccct acggatggtg gtagcttccg gacgatatga ttcgcgcacg
     1381 tggagcagtg tccacttcag ggacgtcgag atgagcctcc tgcacagacg atgacaggag
     1441 cacgtctgca ggtccttctg gtttccagtc cttaggcatg ccgctctgcg ccacagccct
     1501 ccggatcggg tgaaaagagg tgtatcctca acaaacagga ggggtgcctc gcggtatacc
     1561 ctgtcatcag tgcgcgtacc ggcaggcgtc atagggcggt gacgtcaatg cgctgcatta
     1621 ccaggaaggc atgaacgggt gaggtggaac gaaatctttt cgtcggtcga cgcccatgtg
     1681 gcgggccagg ccctgcgctt gatcaccttt ggcgtgccaa aacttggtga cgcaccgatc
     1741 cgcaagcaat atgaggagat gcaggggaaa tatgaccaca ttcgctcctg gttactctcc
     1801 gagccgcgtg gctattcggg aatgaccggt gctgtgttgc ttccctccca cgatcccaaa
     1861 atcgattatg gggtgatctt catgtcgagc gcggggtaca gcccgatctc aggccatggt
     1921 ttgatcgcac tgtcgacggc cctgatcgag actggggcgg taccgattga tggcccggac
     1981 acgaggatta cattcgacac ctttgttggc ccggttcagg ctcgtgcaag tgtcgaccag
     2041 ggacgcgtta gaggggtgcg cttccgcaat gtgccggcgt ttcgtctggt gcatgacctc
     2101 ccgatcgaga tcaacggtca aacggtgccg gtagatatcg cctatggcgg gaactggtac
     2161 gccatagtga aggccgacga tcttggggtg tcgctggagg cttcgcaggc ggcagagctg
     2221 gcacaacgcg ggatggcggt gatgcgcgcc agcgaacgtg ctacggatgt cgtccatcct
     2281 caagacccgg acctgtcagg gctgtatgga gtgattattc tcggggaacc caagtccgag
     2341 gattccacga gtcgcaacgc cacagtctat cttggtgggc tgatagatcg gtccccctgc
     2401 gcaaccggga tggcggcaac catggcgtgc ctcgcagccg acgagagact ggcggtaggt
     2461 gcttccttta tctcggagag catcgtcgac acgacgatgt ctggccggat cgttgtgggt
     2521 accgccgtgg gcgagcacca gggaatcgtg gtggaaattt ccggtcgcgg tgcgcttacg
     2581 gggatgcatc agttcttcgt agaccctgct gagtggcgtt ccagcggttt cgtacagctc
     2641 tagcgtgcgg cgatactggc gcacaaacat tcgctcaagg gacgccgaaa ccctggctag
     2701 cggaaagaag tgccactaac cttccgggtt tcgcagctcc gtttgtgaaa ttggtcacac
     2761 acgcatttcg gtggtacact cacgcgtgag accggcgggg tgagcgcaat gtgctccgct
     2821 agcaagcgag ggagcgcggt gggcgacaac aagctaccag acttccgaga acatgaccgg
     2881 gacatacaag ctgtaggaac tgccgctggg ctggggtttt cacttgttgc atctctggtc
     2941 tttttcatcg ccggaggggc gtggttggat agctggctgg ataccgctcc ggtgctaata
     3001 ttggtcggcg ttgccctcgg attgatcgcg gcgggttatc aattgtatga gctcacattg
     3061 ctcggcaagc cggatcgcga gaacggtccc attggccgtg ccctggagcg tcgggcatca
     3121 gcaaaatcca ggcgtcgata ggttccttca tacggtctgc aattagttca gttcgccaag
     3181 ctctcactgt cttcaaaaga gttgattcgc gggtcaggaa tgcacgagca agattgcatt
     3241 ccagcgcata cgagcgcatg ccagggttga ggagaggcgc cagcacgaca tggaactgca
     3301 tatttcgctt gcggcagagg cactcttcca tttgggcccg atagcggtca ccaattccat
     3361 gcttgtcatg tttttggtga tggcggctat cctgcttgtt ggttcctggc tggcccgaaa
     3421 ggcgcagaga gatccccatc cgggtcgcgc ggtgggtctc gctgagatgc tcgtcgagtt
     3481 cttgctgaac ctggtggagt caactgcagg caagcggatc gggcgccgca tcttcccgct
     3541 gatcgccggc atctttatct tcgtgttgtt cgccaacttc actggtcttt tgcccggtgt
     3601 aggcaccatc tatatcaagg aagacgccga ggaagtggtt tccggtgagg aagctaccct
     3661 ggagaccacc gaagagcact cggaagagga gcaggtggcg ggagaggccg tcgcggctga
     3721 cgagcatgct gctgaagaag aacaccatcc ggtaaatgtg ccgttgctcc gcccccctac
     3781 agctgatttg aacatgacgt tggcgatgtc ggtattgacg ttcacggttg tccagatcgc
     3841 cggtgtctcg gcccacggtg tgcgtggacg tatcaagcac atggcgaacc caccgttcct
     3901 tttccccatc gaagttatct ccgaactttc acgcattatc tccctctccg cccgtctttt
     3961 cgggaacgtc ttcgccggtg aagtgctgct tggcgtgatg tacgccattg ccgcctcgat
     4021 caagatcgcg gtcatcccgg tcctggcacc cgtcgtgttc cttggtttgg agacgctgtt
     4081 cggcacaatc caggcgctgg tctttgccct gctgaccttg atctatgtct ttctcgccgc
     4141 tgccggccat gatgatcacg acgagcaaca tgtcgatgag catgacaccg tgcggccggt
     4201 agaggctcag gcccacagct ccgggagcat gccggcatct gcaggcgact aaactttgcg
     4261 ggttcagcca tggggaaggc cccagggttc gggttcgaag cgttggtttc ttttggatag
     4321 agcgacatag ctctagcaat gggttgatgg ggccgccggc tggatcgcgc ggcaccgaca
     4381 ggaggaagca tcacgtgttt ggtggtatta cagatcccga ggccgtacgt tacattggct
     4441 cgggaattgc aatcggcatt ggcggtcttg gcccggcgct cggtatcggt atggccgtgc
     4501 gtggcgcgat ggaggccatt ggtcgaaacc ctgaggctgc tgggca
//
LOCUS       DASYWM010000071         7083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_377786, whole genome shotgun sequence.
ACCESSION   DASYWM010000071 DASYWM010000000
VERSION     DASYWM010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377786"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /gene="heR"
                     /locus_tag="VGR29_02850"
     CDS             <1..956
                     /gene="heR"
                     /locus_tag="VGR29_02850"
                     /inference="COORDINATES: protein motif:HMM:NF037099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HEV2072557.1"
                     /translation="PTLSQIQLLPQLPVDVRSPMLQGTGFLEQWLDWRKACPLHAQHA
                     RDKKLDGMAATTALPGDLEMADSEASRLRRYNVVVGLLHLAQAIAMVVLSTDFTLPAT
                     RTIPAGPPGTLPGQEIWFNLPLGPAVAAFLFLAALDHLLMASPGVNGWYNRMLVNERN
                     YARWIEYSVSASLMVVLIAMLTSVTDIGALIAIAGVNASMILFGLLQEAFVRPSRDRV
                     NWMPYLFGCFAGAIPWIVIALHLGFAETSSSAGGPPTFVYGIIVSLFLLFNTFSINMV
                     LQYRQVGRWRSYLYGERVYIIISLIAKSALAWQVFANVLVG"
     gene            1065..1391
                     /locus_tag="VGR29_02855"
     CDS             1065..1391
                     /locus_tag="VGR29_02855"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEV2072558.1"
                     /translation="MIALLENKREEIARLCKQFGIRRLEVFGSAATGAFDPESSDLDF
                     LVDLGGYERGVAKRYLRFAEALEQVLGFDVDLVTEDSIANPYFRQSMGQNRELVYGTR
                     DREAVA"
     gene            1549..1728
                     /locus_tag="VGR29_02860"
     CDS             1549..1728
                     /locus_tag="VGR29_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEV2072559.1"
                     /translation="MRIPELRDIVNTRNRITHGYNAVNFNLLWDIVPDDIAPLRDSMA
                     ALLREAPPPGKNLEA"
     gene            complement(1739..2881)
                     /locus_tag="VGR29_02865"
     CDS             complement(1739..2881)
                     /locus_tag="VGR29_02865"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEV2072560.1"
                     /translation="MDNYRDCAVDAPEGTTLTQRQLNYALLARQLLLERSPMQVTDAL
                     EHLVGMQSQVPSDPYIGLWSRLEGFATDELSRIMQDRAAVRASLMRGTIHLVTVADYL
                     VLQPVMQALHERVFPRTDQGKRLDPAHVPEILEAGREMLAETPLSMKALAARLAERWP
                     DRDPGGMAQAVRFLLPLVQVTPRGIWGASHGATWAIAEQWIGQAIPNSSTPDAVLLRY
                     LAAFGPATVADMQAWSGLQGVRAVVEHMRPHLRVYRNEKGQELFDVLGAPLPDPESPA
                     PVRFLPGFDNALLSHADRTRIIAEEHRKAIGSRNGLFLSTVLVDGFVAATWGIEKTKG
                     KAVLRIVPFTALSPRDHDAIQAEGERLLVFLAGGLSHEVVIASGAG"
     gene            complement(2977..3474)
                     /locus_tag="VGR29_02870"
     CDS             complement(2977..3474)
                     /locus_tag="VGR29_02870"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEV2072561.1"
                     /translation="MMQETVSDDETQSLQKAEAQDGDLSDLLEELRVLLPGTQTLTAF
                     LIILPFNPGFREIRDEQKVVYIITFLCSLISLMLFTAPAAHHRQQRPLRDRETFKNYA
                     TRFMIAGLIPLSIALVLAAQLVLFAVVRDRWISWTIAGVLAILVLALWWIFPKVTRDK
                     ISGVH"
     gene            3584..4378
                     /locus_tag="VGR29_02875"
     CDS             3584..4378
                     /locus_tag="VGR29_02875"
                     /inference="COORDINATES: protein motif:HMM:NF037778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEV2072562.1"
                     /translation="MIDQEFIAAWVDRLRRSIPDAAAILLKGSYARGEPGPFSDVDFD
                     VLVDPGPRDDYLTYLVQDDGGQLVHVSVAVQDVESWLSEADEVEDWAYRLTALETTLL
                     LWAREESLRRRLDRPARQHPAGDPELEDFMEAHSKVRNALARGDDLAIRIAAQTLGTL
                     CPSLLRPINPEVQPTHRRAALLAVLDFPVVPKGYRDDLLLCLGLSGRESTLEDVHDAA
                     NRLTTGTLSLLRRHAELLQSALPTDLYGYLQDGTLERYIRQREHRP"
     gene            4375..4761
                     /locus_tag="VGR29_02880"
     CDS             4375..4761
                     /locus_tag="VGR29_02880"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEV2072563.1"
                     /translation="MNESELVIRVVAMPADTNSSGDIFGGWLLSQMDLAAWVCARKLT
                     SHHLATVALDGVIFHRPVYVGDSVLCYAKVEKTGRTSITVRIDAMVERKDGGAEEQVT
                     EGRFVLVAIGEDRKPVPLQEESLGNR"
     gene            complement(4806..5786)
                     /locus_tag="VGR29_02885"
     CDS             complement(4806..5786)
                     /locus_tag="VGR29_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072564.1"
                     /translation="MISQRSLSSTPERVIIVLLIILVALATYGGAAFGMNQEADSPSV
                     VEREWPEGVELLAAQELDLEAGEYQWQITTLTAPAENAEPLEVRHGVLIALNGAILVG
                     FDDDEVIRLNTGAALPLQDEDEEAEIAVTSAGDAPVEFLVVELVNLDDVEPDDTENEV
                     GPLTVPEGAFTLVLVNLPAAVTDDTTAEQVIEDALRPGVLISHTDEGIPAEIVAGQEY
                     DRWIVALYPSTDSPTATPTPTVAPPQQQAPPRTTLPTATSTPTATVTPTATATATTTP
                     TATATSTPTATATATATSTPTMVPPTNTPVPTPTPTMVPPTNTPEPTAAT"
     gene            complement(6197..6775)
                     /locus_tag="VGR29_02890"
     CDS             complement(6197..6775)
                     /locus_tag="VGR29_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072565.1"
                     /translation="MDSLYSRRSLTWGLALLGIAASIGSRTRDVAALQSNGIQSGGIG
                     LSRSEWENIYGPGEATQSHGLYVEPTFGGPIYAGFDFSAFEDGLLEFLEFQWTNVSQS
                     GGLSPEDTVARVSAALLADAQHRETYFMGATPGGPITLRSERWTSRELDMVTNGRASI
                     LVTCQEKPISFPGTDRPNATAVTVATIAVEQA"
BASE COUNT         1354 a         2146 c         2132 g         1451 t
ORIGIN      
        1 gaccaacgct gagtcaaatc caacttctcc cgcaactccc ggtcgatgtc cggtcgccta
       61 tgcttcaggg aacagggttt ctcgaacagt ggttggattg gcgcaaggca tgccctctcc
      121 acgctcaaca cgcacgtgac aagaagttgg atggcatggc agcgacgacg gcactacctg
      181 gtgacctgga gatggccgat agcgaggcca gccgtttgag gcggtacaac gttgtcgttg
      241 gcctgcttca tcttgcgcag gctatcgcca tggtcgtgct cagcacagat ttcacccttc
      301 ctgccacccg gacaataccg gccgggcctc caggtaccct cccgggtcag gagatctggt
      361 tcaaccttcc ccttggacct gcggttgcgg ccttcctttt tctggccgcc ctggatcacc
      421 tccttatggc atccccgggg gtgaacggct ggtacaaccg catgctcgtc aacgagcgca
      481 actatgcgag atggatcgag tactcggtca gcgcatcact gatggttgtt ctcatcgcca
      541 tgttgacctc ggtgacggac atcggcgccc tgatcgcgat tgcgggtgtc aacgcctcga
      601 tgatcctttt cggtctgcta caagaagctt tcgttcgtcc cagtcgcgac cgagtgaact
      661 ggatgcccta cctgtttggc tgcttcgccg gcgccattcc ctggatcgtc attgcccttc
      721 atcttggctt cgcagagacc agcagctcag caggcggtcc gccaacgttc gtttacggca
      781 tcatcgtgtc gctgtttctc ttgttcaaca cgttttcgat caatatggtc ctgcaataca
      841 ggcaagttgg gcgctggcgc tcctacctgt acggtgaacg ggtctatatc atcatcagcc
      901 tgatcgccaa gagcgccctg gcatggcagg tgttcgcgaa cgtattggtg gggtgaccca
      961 ggcaatatgt gtgaaggtgg gaatcccggc tgtcgacgct ccgttcacta caatcaacga
     1021 acggtgcgcg cgcctctcct atccaacctt cattggagta cgccatgatt gccctcctcg
     1081 agaacaagcg agaagagatt gcccggctct gcaagcagtt cggcatccgg aggcttgagg
     1141 tttttgggtc ggcggccact ggtgcgttcg atccggaatc cagtgacctg gattttctcg
     1201 ttgatcttgg tggatatgaa cggggcgtag cgaagcggta cttgcgcttc gcggaagcac
     1261 tcgagcaagt actcggattc gatgtagatc tcgtcaccga ggattcgatc gcaaatccct
     1321 acttcaggca gtcaatgggc caaaaccggg aactcgtcta tggaactcga gaccgcgaag
     1381 cggttgcatg atgccatctc agcatgcgat gaaatccagg attttgctga gggtagctcg
     1441 cgatcgcaat ttcttgagga ccgcggatgg cagttggtgg tttgaaagct gatcgagatt
     1501 gtgggtgagg cacttctgca ggctgaaata gcagaaccat cactgtctgt gcgtatccct
     1561 gaactacgag acatcgtgaa tactcgaaac cgcattaccc acggctacaa cgccgtcaac
     1621 ttcaatctgc tctgggacat tgtgcctgac gacattgcgc cgctacggga cagcatggcc
     1681 gcattgctgc gagaagcacc gccgcctgga aagaacctgg aagcatgacc ggagcttcct
     1741 atcctgctcc gctcgctatg acgacttcat gacttagccc gcctgcaaga aacaccagca
     1801 atcgctcgcc ttctgcctgg atcgcatcgt ggtctcttgg ggagagcgct gtaaacggca
     1861 caatccgcag gactgccttt ccctttgtct tctcgatccc ccaggttgcc gcgacgaaac
     1921 cgtcaacgag caccgtggag aggaagaggc cgttccgaga gccgatcgcc ttgcggtgct
     1981 cttcagcaat gatccgggtc ctgtccgcgt gcgagagcaa ggcgttgtca aagccaggca
     2041 agaaccgaac gggagccgga gactccggat caggcagcgg tgcacccagc acatcgaaga
     2101 gttcctgccc cttttcattc cgatacaccc gcaggtgcgg ccgcatgtgc tcgaccaccg
     2161 cccgcacacc ctgcaacccc gaccatgcct gcatgtccgc caccgttgcc gggccgaagg
     2221 cggcgagata gcgcagcagg accgcatccg gcgtggagct gttggggatg gcttgcccga
     2281 tccactgctc ggcgatcgcc cacgtcgctc catggctggc tccccatatt ccacgcggtg
     2341 tcacctgcac gagtggcagc agaaaccgca ccgcctgcgc catcccgcca ggatcccggt
     2401 ccggccagcg ctccgccagc cgggcagcca atgctttcat gctgaggggc gtctccgcca
     2461 gcatctcccg tcccgcttcg aggatctccg gtacatgagc cgggtcaagc cgcttgccct
     2521 gatcggtccg ggggaacacc cgttcatgga gtgcctgcat caccggttgc aacaccagat
     2581 agtcagccac cgtgacgaga tggatcgttc cccgcatgag cgaggcgcgg accgccgcgc
     2641 gatcctgcat tatccgggag agctcgtccg tcgcaaaccc ttcaagccgg gaccacaacc
     2701 cgatataggg atctgacggt acctgcgatt gcatcccgac cagatgctcc agcgcatccg
     2761 tcacctgcat cggagatcgt tccagcagca gttgccgggc gagcagcgcg tagttcagct
     2821 gccgttgggt tagcgtcgtg ccctccggag catcgacggc gcaatcccgg tagttatcca
     2881 tccgatctcc cccacccatc tacgccttca gacatggtcg cagcgtagca aggaaggggg
     2941 ccaatggctg tcctgattcg tgtcgggcag ggtgtgtcaa tgcaccccgc ttatcttgtc
     3001 tcgtgtcacc ttcgggaata tccaccacag tgccagtacc agaatcgcca gcacccctgc
     3061 tatcgtccac gagatccagc gatctcgtac cacggcgaac agcacgagtt gagctgcaag
     3121 caccagggcg atggaaagcg ggatgaggcc tgcgatcatg aacctcgttg catagttctt
     3181 gaaggtctcc cggtcccgta acgggcgctg ctgcctatgg tgtgccgccg gggcagtgaa
     3241 gagcatcagg ctgatcaggg agcataagaa ggtgattata tagacgacct tctgctcgtc
     3301 gcgtatctcg cggaacccgg gattgaatgg caggataatc agaaacgcgg tcagcgtctg
     3361 tgtaccgggc aggagaacac ggagctcttc cagcaggtca gaaagatcac catcctgtgc
     3421 ctcagccttc tgaagcgatt gcgtttcgtc gtcggacaca gtctcttgca tcattctccc
     3481 agtgcctgtg agttcatcgg gtttgttccc tggttgctga ttcgaccaga gcgggcggcg
     3541 cctgattgat ctttaacgat ctgtcgatca ggatggaatg tacatgatcg atcaagagtt
     3601 catcgcagct tgggtcgacc ggctccggag aagtatcccg gacgccgctg ccatcctgct
     3661 caagggtagt tacgcacgtg gcgaaccggg accgttcagc gatgtcgact tcgacgtgtt
     3721 ggtcgatcca ggaccgcgcg atgactatct cacatacctg gtccaggacg acgggggcca
     3781 actcgttcat gtgtcggtcg cggtgcagga tgtcgagtcc tggctgtccg aggccgatga
     3841 ggtcgaggac tgggcctacc ggctcacggc gcttgaaacg acgctgctcc tctgggcgcg
     3901 ggaggagtcg ctgcgacgaa ggttggaccg tcccgcccgg caacatccgg ctggagaccc
     3961 ggaattggag gacttcatgg aggcccacag caaggtgcgt aatgcgctgg cgagaggtga
     4021 cgatctggcg atccgcatcg cagcacagac gctgggcacg ctctgtccct cgctgctgcg
     4081 accgatcaac cccgaggtgc agccaaccca tcgccgtgct gcactcttgg ccgtgctgga
     4141 ctttcccgtc gtacccaagg gctaccgtga cgacctgctg ctctgcctcg gactttcagg
     4201 acgggagagc acattggaag atgtccacga cgctgcgaat cgtctcacga ccggcacgtt
     4261 gtcgctcctg cgccgtcatg cggagttgct gcaatcagca ctgccgactg acctgtacgg
     4321 ctacctgcag gatggcacac tggagcgata tattcgccag agggaacacc gaccatgaac
     4381 gaatccgagc tcgtcattcg cgtcgtcgcg atgccggcag acaccaactc gtccggcgac
     4441 atcttcggcg gttggctgct cagccagatg gaccttgccg cctgggtgtg cgcccgcaag
     4501 cttacgagtc accatctcgc gacggtggca ctggacggcg tcattttcca ccggccggtg
     4561 tacgtgggag attccgtcct ctgctacgcg aaggtcgaga agaccggccg caccagcatt
     4621 accgtgcgaa tcgacgcgat ggttgagcgc aaggatggcg gtgcggaaga gcaggtgacc
     4681 gaggggcggt tcgtcctcgt tgctatcggt gaggatcgca aacccgtccc acttcaggaa
     4741 gagtccctgg gcaaccgcta aatccgggtg cggaacagtt caagccgcac ccggatggat
     4801 ctcccctaag tggccgccgt cggctcagga gtgttggtcg gcgggaccat cgtcggagtt
     4861 ggtgtcggca caggagtatt agtcggcggg accattgtcg gtgtcgatgt ggcggtggcc
     4921 gtggccgtgg ccgtcggtgt cgacgtagcg gtcgcggtag gtgttgttgt cgcggttgcc
     4981 gtagcagtgg gggtgaccgt ggcggtcggt gtggatgtcg cggttggcag tgtcgttctt
     5041 ggcggagctt gctgttgcgg tggcgccaca gtcggcgtcg gagtggcggt tggggagtcg
     5101 gtcgacggat agagggccac gatccagcga tcgtattcct gacccgcgac aatctctgcc
     5161 gggatgccct cgtcggtgtg ggagatcagg acacccggcc tcagcgcgtc ttcgatcacc
     5221 tgctcggcag tggtgtcatc ggtaacggcg gcaggcagat tcaccagcac cagcgtgaac
     5281 gcaccttccg ggacggtcag cggtcccacc tcattctcgg tgtcatcagg ctcgacatca
     5341 tccaggttca ccagctcgac caccagaaac tcgaccgggg catcgcccgc actggtgacc
     5401 gcaatctccg cttcctcatc ttcgtcctgc aggggaaggg cagcaccggt attgagacgt
     5461 atgacctcgt catcatcaaa cccgacaaga attgccccgt tgagcgcgat caggacgcca
     5521 tggcgcacct cgagtggctc ggcattttcg gccggggcgg tgagcgtggt tatctgccac
     5581 tggtattcgc cagcctccag gtcgagctct tgggcggcaa ggagctccac gccctccggc
     5641 cattccctct ccacgacgga aggggaatct gcctcctggt tcatgccaaa tgccgcaccg
     5701 ccataggtcg ccagcgccac gaggatgatc aggagcacga tgatcacacg ttccggggtg
     5761 gaggagagcg agcgttggga gatcatggtc tgggtgcctc tttcaatagc gtgtgcgtca
     5821 tgttggatgg aattggtcag gcgtaggatc agcatcataa gcgaggaaca catgcgacgg
     5881 ggatgtgaga accttgcaag ccactttaat gaggggttgg ccacgatgaa accgattcag
     5941 ggcgacctac aggcaccagc atcgttacca tgtcgatatc cctgcgcgca cgacgcttcg
     6001 ccgcctgccg agactttggt tgcctgtcta ctcatgcttc cggtgggcct gaaaatcatc
     6061 ttagcacgag agtcgtcaat gcagcgctcg atcgacccac gagcgtgaac ctgcctgttg
     6121 accggcccgc caccccggat tttcgggcag gcgattccat ctcccggctg tgctgcggtg
     6181 cagtgaaaca cgtgtgctac gcctgttcaa cggcgattgt agcaacggta accgccgttg
     6241 cgttgggccg atctgtaccc gggaaggaga tgggcttttc ctgacaggtc accaggatcg
     6301 acgcccggcc gttcgtcacc atatcgagct cgcggctggt ccagcgctcc gagcgtaacg
     6361 tgatcggccc cccgggtgtc gctcccatga aatacgtctc cctatgttgc gcgtcggcga
     6421 gcagggccgc actgacgcgg gctaccgtgt cctccggcga aagaccgcca ctctggctga
     6481 cgttggtcca ctgaaactcg agaaattcga gaagtccatc ctcgaaagca gaaaagtcga
     6541 atccggcata gattggtcca ccaaaggtgg gctcgacata gagcccgtgg ctttgcgtcg
     6601 cctcgccggg cccgtagatg ttctcccatt cggagcgtga caacccgatg cctcccgact
     6661 ggatgccgtt cgactgcagc gcagccacat cccgggtgcg ggagcctatg ctagcggcaa
     6721 tccccagcag ggcaagaccc caagtcagcg aacgacggga atacaacgaa tccatgagaa
     6781 cctcctgaga tggatgggcg cggaggcata tggccgctcg cgtcatgaat gtgtggggtc
     6841 ccgctttcct ggaagggacg gcgcgttcgg actaccttcc accgtgccgt tccttctctc
     6901 tcgctacaac ggttatccac ctcatgtcgt tccgcgctcc cgggagttcg tatacaatcg
     6961 gcgaagtttg ggacatcgcg tgtctaaagg ggagcttctc aacatggcgg acatcgtacg
     7021 catgggcgtt gtcggcgccg ggaccatcgc ccagcgcggc atcatgcctc acctcagcca
     7081 gga
//
LOCUS       DASYWM010000072         5233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_584516, whole genome shotgun sequence.
ACCESSION   DASYWM010000072 DASYWM010000000
VERSION     DASYWM010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584516"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..999)
                     /locus_tag="VGR29_02895"
     CDS             complement(160..999)
                     /locus_tag="VGR29_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2072566.1"
                     /translation="MSHEVIGEIGVKYGRGGGRPLHLDILRPAEPSRSLRPAVIYVHG
                     GGWLGGERTSTPNQILAEAGFVTVSISYRFSSEAIFPAQIHDVKASIRWLRANAGRYG
                     VDPERIGIWGHSAGGHLAALAAVTGNNPDFEGEGGNPDQDSSVQAAVPISAPLEFLID
                     WYAVAQMPVHPEAWDCISGLMGGLPHTVPEMARLSSPYWHVHATAAPQLVIHGEMDDL
                     VPIGQARAYVASLRRYAGIDASLIALEGVGHEAHSSLFPNQPDPSGLQARVTELFRDH
                     LGQ"
     gene            1234..2046
                     /locus_tag="VGR29_02900"
     CDS             1234..2046
                     /locus_tag="VGR29_02900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2072567.1"
                     /translation="MTDAPTLHGHHPRIGHNRLRGKTALVTGSTRGLGRTTAEWLARE
                     GASIYVSGRKHEDVQATIAAIEMLGVKAWGSAADLAKPEETHALAIRALETAGPFDIL
                     VNNAGMSQPQSFLDVTDAEWDIELNTNVRSPFILSQHLAREMIAHSIRGRIVNISTIG
                     VFAAHKQQMVYNIAKGGVQAMTRCMAWELGQHGISANCVAPGAVPERPGSREQMNSGA
                     FQVWSERIPMGRLGRAEDIAAAVVFFCLPESEWTTGQTLLVDGGHISYLRED"
     gene            2059..3219
                     /locus_tag="VGR29_02905"
     CDS             2059..3219
                     /locus_tag="VGR29_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072568.1"
                     /translation="MNAGLFTYAWDLEAEGYDVAVGRIAEAGFTSINLATSYHAGKFL
                     LPRNPRRRVYFAEDGALFFRPDPSRYGRIQPRVHSLAQQDDDPVTKLLAAIRPYNLAY
                     TAWTVCLHNSWLGERYPDTTMHTAFGDPLIHSLSPAHPDIRAYMVAMVTDLVSRYEAA
                     AIQLESPGYMGFLHGYHHEIIGADLDAVQQQLLAISFNPVELAGAREAGIDAERLRSW
                     VAGLLDACWNQGVPLMDGDSPASGVQDLFDDAEFMGYRTWQADQVVSLSQEIRDAVKA
                     ANFQAQIFHFAALDGSDADERLIATGDGILTGYASSDEDATRRAKRAAACGKRVHGQI
                     RAIAPDTTDPAMIASRMEAWRASGVDGVDIYNYGLMPGVMWDAVVETVKGLQ"
     gene            3216..3680
                     /locus_tag="VGR29_02910"
     CDS             3216..3680
                     /locus_tag="VGR29_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2072569.1"
                     /translation="MIDSVIVRDVEPADGARWRDLWKDYCDFYGTTMPEAVTDETWRR
                     ILDVDTPSFGAIVAERDDSIIGFANYVLHPYTWAIGDQCLLHDLYVAPDVRGGGVGEQ
                     LIRFLQHRGAERGWTHLYWLTHETNARARRLYDRFAPASGFIRYTVPVEEAT"
     gene            3677..4129
                     /locus_tag="VGR29_02915"
     CDS             3677..4129
                     /locus_tag="VGR29_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2072570.1"
                     /translation="MTDVRFRQATIEDIQAVVDMLADDALGATRETPDDLTPYRDAFA
                     AIDADPHQLLIVCEGGGQAGGTMQITFIPGLSRKGSTRALIEGVRVHRDARGTGLGTK
                     MMQWAVEEARARGCAMVQLTTDASRTDAHRFYERLGFQKTHLGFKMVL"
     gene            complement(4306..4851)
                     /locus_tag="VGR29_02920"
     CDS             complement(4306..4851)
                     /locus_tag="VGR29_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072571.1"
                     /translation="MDRRDSSIPALVITGPVGAGKSTMMRALSELLEQRSIRHVGIDM
                     DNLRWVYPSPPGDRFSVRLGFNNLAAMWPNFQEMEPECVVIADVVESPAEIDDYLAAM
                     PGTRVTVIRLDVPMPVILQRLEGRECVHRVEWYRQRAPELQEIMERNHIGDLVIAAGD
                     RAPEDIAHEILQRVDPLQGQA"
     gene            4917..>5233
                     /locus_tag="VGR29_02925"
     CDS             4917..>5233
                     /locus_tag="VGR29_02925"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HEV2072572.1"
                     /translation="MTVDPMPRNKFDQEARLELLRHAASAYIDTAISNATQEYPHMAW
                     IVASGPESYRTHRELHPAFYGSFDWHSCVEMHWTAVRLLRLFPRPGTEDRARQTLSEL
                     LTP"
BASE COUNT         1018 a         1665 c         1551 g          999 t
ORIGIN      
        1 gttcagtatc cttacttata gttgggacat agagaagtga gaccaacgct tttctataca
       61 tctctcctcc ccccacccca atggccagcg gataatccga gttcggattc ctccgctggc
      121 tgttattttg taccgcatac ccacctcccg tgatcctgct cattgcccca gatgatctcg
      181 gaaaagctcg gtgacgcgtg cctggagacc cgaaggatcg ggctgattcg ggaagagcga
      241 gctatgcgct tcgtgaccga ccccttcgag ggcgatcagg ctggcatcga tcccggcata
      301 gcggcgtaag gatgcgacgt aggcacgcgc ctggccaatt ggcacaaggt cgtccatctc
      361 gccatggatc actaattgag gtgcggctgt agcatgcaca tgccagtacg ggcttgagag
      421 ccgggccatc tccggaacgg tatggggaag gccgcccatg aggccgctga tgcaatccca
      481 cgcttccgga tgcacgggca tctgcgccac tgcataccag tcaatgagga actccagcgg
      541 ggccgaaatc gggacggccg cctgcacgct gctatcctgg tccggattgc caccctctcc
      601 ctcaaaatct ggattgttgc cagtcaccgc tgccagggcg gccagatgcc ctccggctga
      661 gtgaccccag atcccgatgc gttcgggatc caccccgtac cggccagcgt ttgcgcgcag
      721 ccagcgaatg gaggctttca cgtcgtggat ctgggcggga aagatcgcct cgctgctgaa
      781 ccggtaactg atggagacgg tgacgaaccc cgcctctgcc agaatctggt tcggtgtcga
      841 tgtccgctcg ccacccaacc atcccccgcc gtgaacgtag atcacagccg gtcggaggga
      901 ccgggagggc tccgccggtc ggaggatatc gagatgaagc ggacgccctc ccccacgccc
      961 gtatttgact ccgatttcac caatgacctc gtgtgacatg gccggctatc ctcctgcgac
     1021 aatcgctctg ttgattctcg atcgggcacc tgcgctctac gcgagcctat cgacgtcctc
     1081 ctgattgtcg cacccggtca aagccgggaa atcactccgg caccgggacg caggagccgc
     1141 caaaattctc cggtacaatc ccgtatatcc gtaggctctc catgcccgtc gcgtggcgct
     1201 ggtttggtct catgtccccg gaaggatcgt ccgttgactg acgcacccac attacatggc
     1261 catcaccctc gcattggcca caatcgcttg cgtggaaaga ccgccctggt aaccggcagt
     1321 acccgtggtc ttggtcgcac cacggccgag tggttggcac gggaaggcgc cagcatctat
     1381 gtctccgggc ggaaacacga ggacgtacag gcgacgattg ccgcaataga gatgctcggg
     1441 gtaaaggcgt ggggatctgc ggctgatctt gcgaaaccgg aagagacaca tgcacttgcg
     1501 atccgtgcgc tggaaacggc gggtccattc gatatcctcg tcaacaacgc cggcatgagc
     1561 cagccccagt cctttctcga cgtgacagac gcagagtggg acatcgagct caacacgaac
     1621 gtccgcagcc cctttatcct cagccaacac ctcgcccgcg agatgatcgc gcacagcatt
     1681 cgcggacgca tcgtcaatat cagcacgatc ggcgtcttcg ccgcccacaa gcaacagatg
     1741 gtctacaaca tcgccaaggg cggcgtgcag gcgatgaccc gctgcatggc ctgggagctg
     1801 ggccagcacg gcatcagcgc caattgcgtt gctcccggcg ccgtgcctga acgtccgggt
     1861 tcccgggagc aaatgaactc tggcgcgttc caggtctgga gcgagcgcat ccccatggga
     1921 cgcctcggtc gggccgagga tattgctgcc gccgtcgttt tcttctgcct gccggaatcg
     1981 gaatggacca cggggcagac actgctcgtc gacggcgggc atatctccta tctacgcgag
     2041 gactaaggag gcgtcatctt gaatgcggga cttttcacct atgcgtggga cctcgaagcc
     2101 gaggggtacg atgtggcggt cgggcgcatt gccgaggctg gcttcacctc gatcaatctc
     2161 gccacctcct atcatgccgg caagttcctg ctcccccgga atcctcgccg ccgggtctac
     2221 ttcgcggaag atggcgccct cttcttcagg cccgacccca gccggtacgg ccgcatccag
     2281 ccacgcgttc actccctcgc ccagcaggat gatgaccctg tcaccaaact gcttgccgcg
     2341 atccggccgt acaatctcgc gtacaccgcc tggactgtct gcctgcacaa cagctggctg
     2401 ggagagcgct atccggacac gaccatgcac accgccttcg gtgatccgct catccattca
     2461 ctgtcgccgg cccatcccga tatccgcgcc tatatggtag cgatggtgac ggatctcgtc
     2521 agccgctacg aagccgccgc gatccagctg gagtcccccg gctacatggg gttcctgcac
     2581 ggttaccacc acgagatcat tggcgccgat ctggatgccg tccagcagca gttgctggcg
     2641 atctcgttca acccggttga gctggctggt gcccgtgaag cgggaattga tgctgaaaga
     2701 ctgcgctcct gggtggcagg tctccttgac gcttgctgga accagggcgt gccgttgatg
     2761 gacggggatt cccctgcttc cggagtgcag gacctgttcg acgacgccga gttcatgggg
     2821 taccggacgt ggcaggcgga tcaggtcgta tcgctgtcac aggagatccg tgacgcggtc
     2881 aaagcggcca actttcaggc ccagatcttt cattttgccg cgctcgatgg ctcagatgcg
     2941 gacgagcgat tgatcgccac tggcgacggc atcctgacgg ggtatgcatc gtcggacgaa
     3001 gatgctaccc gccgggcgaa gagggccgca gcgtgtggca agagggtaca cggccagatt
     3061 cgcgcgatcg cgcccgacac caccgatccg gccatgatcg cttctcgcat ggaagcctgg
     3121 cgtgcaagcg gcgtcgatgg cgtggacatc tacaactacg gccttatgcc cggcgtcatg
     3181 tgggatgccg tcgttgagac cgtgaaaggc ttgcaatgat cgactctgtc atcgttcgtg
     3241 acgtggagcc ggcagatggg gcgcggtggc gggacctttg gaaggactac tgcgacttct
     3301 acgggacaac catgccagag gctgtcaccg acgagacatg gcgtcggatt cttgatgtcg
     3361 atacgccgtc gttcggcgca attgtcgccg agcgggatga ctccatcatt ggattcgcga
     3421 actatgtcct ccatccctac acatgggcga ttggcgatca gtgcctgttg cacgacctct
     3481 acgtcgctcc cgatgtccga ggcggcggcg tgggagagca gcttattcgc ttcctgcagc
     3541 accgcggtgc cgagcgtggg tggacgcatc tctactggtt gacgcacgag acaaacgcac
     3601 gagcgcgacg cctgtacgac cgctttgccc cggcaagtgg gtttatccgc tatacggtgc
     3661 cggtcgagga ggcgacatga ccgatgtgag attccggcag gcaacgattg aggatatcca
     3721 ggcggttgtc gacatgcttg ccgatgatgc cctcggcgcc acccgggaaa caccggacga
     3781 cctgacacct taccgggacg cctttgcggc gatcgatgca gacccgcacc aactcctcat
     3841 cgtctgcgag gggggaggcc aggctggcgg gacgatgcaa atcaccttca ttcccgggct
     3901 ctcccgtaag ggctcgacac gcgcactgat cgaaggcgtt cgagttcacc gggatgcgcg
     3961 cggcaccggc ctcggaacga aaatgatgca gtgggcggtt gaggaggccc gggctcgcgg
     4021 ctgcgcgatg gtccaactta cgaccgatgc atcgcggaca gacgcccatc gcttctatga
     4081 acgactcggc ttccagaaga cccatctggg gttcaagatg gtgttgtaat cgtccaaggc
     4141 aagcatcacc aggctcacag gggccaggaa cctgcggcca tccgccgatc agagccgcgc
     4201 gcgtcggtcg atagagggcc gttgtcatcc ccaccagctt ccgccatgtg gagaccgcga
     4261 cgccagaagg gtcctccagt cccgtacaag tgaaaagcag atccactatg cctgaccttg
     4321 caaggggtca acccgttgca atatctcgtg tgcgatatct tccggggcgc gatcgccagc
     4381 agcgatcacc agatcgccga tatggttgcg ctccatgatc tcctgcaact ccggcgcccg
     4441 ttgccggtac cactcgaccc tatgcacaca ctcacggcct tccaggcgct ggaggatgac
     4501 tggcatcggg acatccaacc gaatcaccgt caccctggtt cctggcatgg ccgcgaggta
     4561 atcatcgatt tcggcggggc tctcgacgac atccgcaatc acaacacatt ccggctccat
     4621 ctcctggaag ttgggccaca tcgccgccag gttgttgaac ccgagtcgga cgctgaagcg
     4681 atctcccggc gggctggggt aaacccagcg caggttgtcc atatcaatcc cgacatgccg
     4741 gatggatcgc tgctcaagca actcggacag cgcacgcatc attgttgact tgcctgcgcc
     4801 gacaggaccg gtaatcacca gcgcaggtat ggagctgtca cgccgatcca atatgcgccc
     4861 cttccttgtg gataatgcgt agaggtaatg ctcatcgcca aatggagaat agccatatga
     4921 ccgtagaccc tatgcccagg aataaattcg atcaggaggc acgcctcgag ctgctgcggc
     4981 atgccgccag cgcctacatc gataccgcta tcagcaacgc cacgcaggaa tatccgcaca
     5041 tggcatggat cgtcgcctcc gggcccgaat cctatcgcac ccacagggag ctccacccgg
     5101 cgttctatgg ctctttcgac tggcactcct gcgtggagat gcactggacc gccgtgcgcc
     5161 tgcttcggct ctttccgcgg cccgggacgg aggaccgtgc ccgacaaaca ctaagtgaac
     5221 tgctgacgcc aga
//
LOCUS       DASYWM010000073        21400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_283866, whole genome shotgun sequence.
ACCESSION   DASYWM010000073 DASYWM010000000
VERSION     DASYWM010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 21400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21400
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283866"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..805
                     /locus_tag="VGR29_02930"
     CDS             296..805
                     /locus_tag="VGR29_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072573.1"
                     /translation="MPPWAALAFSQLAGAVSRLGDRQRAMDLGKQGLLKQQEAGSGPG
                     MALALVYLGDIALDWGDYAEAHIRYREGLAQMWQTGDRWHLLHALAGFSIMKAQVGPA
                     EAAARLFGAQAAARAATGHQIPPRYRDSFDAAVVKLRATLGEEAFSTSWTEGMALELE
                     EAVAEALES"
     gene            complement(1092..1376)
                     /locus_tag="VGR29_02935"
     CDS             complement(1092..1376)
                     /locus_tag="VGR29_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072574.1"
                     /translation="MKRAFCAAVFVLALTLASGGDVAAHRITITTPAGEVVDNKDLAR
                     NITPRLFDEDGNNVGKDEGTFSAASRGTNTACEAVPEGGPVRITGGTCHE"
     gene            1493..2374
                     /locus_tag="VGR29_02940"
     CDS             1493..2374
                     /locus_tag="VGR29_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV2072575.1"
                     /translation="MIERPSLQALALFLAVVDHGTMTAAAEAEGIAQPAISVHVRNLE
                     RFYGVPLMERSGRRVRPTATGELVASYARRIAGLVNDLGHAVEDLEGLRTGRLVVGVS
                     ATVAETWLPGMLGRFRRAYPAIEMDVRIGNSEQILHEVSERSLGFGIVGRPDPGPDLV
                     ARRLFSDGLEFFAAAGSSLLARQPLRLDDLADETFVLRETGSATREYMLRCLAAHDMN
                     PVKTIQLGSNEAVKRAVAAGLGVGILSSQTLDVDVRAGDVVILQCAGWECRRHFWLVQ
                     RRDRVLSRAEQAFLDLR"
     gene            2586..5177
                     /locus_tag="VGR29_02945"
     CDS             2586..5177
                     /locus_tag="VGR29_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2072576.1"
                     /translation="MGTRESSPFGSLLRHYRLAAGLSQETLAELTGLSTRGISDLERG
                     ARTAPRLETLRLLAASLGVEGPGLAAFLAARSAVTAEAVPTSQADHAMPPLALTPLVG
                     REREIRAITDLLCDDDVRLVTLTGPGGVGKTRLAQAVAHEVMNQFADGVVFVDLSPVR
                     DAQLVLPTIAAVFGVQDVGAAGVMADALVTALGHQHVLLILDNLEQAIDAAPDIAMIV
                     SRCPWLKVLATSRILLRLTAEQVFPVSPLQLPDGATKPSLPELAQTEAVAFFVNRASA
                     ADLTFHLTEDNSGPVAEIVRRLEGLPLAIELAAVWTRTLPPEALLARLERRLPLLTGG
                     SRDAPLRQRTMRDAIAWSHDLLTPHAQALFRRLGVFVGGFTFEAAEAVTGRDDEGSGV
                     DFLDGIASLIERSLLRREMGPDAEPRYVMLETVREFGLDQLVARGETPAVRAAHATYF
                     LQLAEQSLESFLGPEHRRWLDRVDAEQGNLRAAATWFEEANETDHLLRLARALGHFWY
                     SRGHLTEGRSWLDRALSLATSAPLELGADGLAWASGLAHRQLDIEVAVSLAEASLVEW
                     QKLEGTPPGRARAVLTLAIGLDHRGDKDRAAEFYAEAAEMFRAEGQDSWAGIAIVNLA
                     LVEGGRGRVQLARDLGEQGLSLVQSARSAWGVNHALAVLGDLALDRGDHDEAVQRYHQ
                     CLTASIDVGDPVQMGHDLVRLGVVAATFGDISRAVQLFGAVEALREGAHRTLLPPNLR
                     PKYEAALLKSRESLGENAFKDLWRAGRGWSLGTALTEAAAVTLGAVGSSPPSTGQHPP
                     QAYNLTERELDVLRLMVAGKTDREIAEELFISRRTVTTHVGSLLLKLGVTNRVHAATV
                     AIQDGLL"
     gene            5616..7928
                     /locus_tag="VGR29_02950"
     CDS             5616..7928
                     /locus_tag="VGR29_02950"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2072577.1"
                     /translation="MSLLPRAITPLIGRERELQVVKEFLQRDDVALVTLAGTGGVGKT
                     SLALSVAADLVDDFDDGVTFVSLAPLNDPALVASTLARALGVREAGGRSVVEALRSFL
                     ALRRQLLILDNFEHVLAAAPLVTDLLQFCLGIKVLATSRERLHLRGEQVFEVLPLGLP
                     DQQHLPSLEVLSHVPSVQLFVERGREVQPQFTLAENNTAEVSEICRRLDGLPLALELA
                     AAWIHVLPPASLLTRLERRLPLLTQGPRDVPLRQQTLRDAIGWSYHLLSGAEQRMFRR
                     LAVFVGGFDLPAAEAIAQRVDGMESVSIVTSLVDKSLLRQVEGLVGEPRFGMLEMVRE
                     FGLERLVASGEESAIRQTHASYFLAFAENLRPKIEGPEGPVVLTSLENEHANLRAALE
                     WEIEGADADGALRFVAAMWKFWWVRRHLSNGRALAERALALPGSGPSALRAEASYGAG
                     SLAMGQADYAAAQLHGEAGLALASEIDDPFWTGMPLNLLGNAARLQGDYGKARARYEP
                     ALALYRNLGRTRPFARHAAAMVLVSLGTVAYAEGDLEEAIVRNQEALTIWRDRQDLWG
                     QGTALINLGAVASRQGELCLAESHYRESILCNQTTGDRAGIGEGLAGLALVAASRGLS
                     ERAVRLFAAAEAIREVAGTPNAAMIPADRKWVMTAVKTRLGADAFAAEWETGKALTAE
                     DAVAEAMEPPLSLPGEAPKAKLGDSLGLSGRELEILRLIAEGRSNRDIAGVLFISPRT
                     VTTHTTGIFTKLDVGSRTAAVAVARERGLV"
     gene            8124..8357
                     /locus_tag="VGR29_02955"
     CDS             8124..8357
                     /locus_tag="VGR29_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072578.1"
                     /translation="MSTPKRLVLALILVVLFLGQAGAPAHPDHASCQPLSDQIAKVAK
                     AGAAGEFMRTLALNHQVAEIGAGFPVQFCEPKP"
     gene            complement(8641..9831)
                     /gene="moeB"
                     /locus_tag="VGR29_02960"
     CDS             complement(8641..9831)
                     /gene="moeB"
                     /locus_tag="VGR29_02960"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEV2072579.1"
                     /translation="MTTFRALLDQIRAEIREVDVAGARALMEDGHAAVIDVREPDETE
                     QGIVPEAVTIPRGYLELRVEDAVPDKTAPVVVYCAGGTRSALAAKSLQDLGYTNVASL
                     AGGFNAWKDAGEAWSQPRTLTMDQKRRYSRHVLIPEIGERGQLQLLDARVLLIGAGGL
                     GSPAALYLAAAGLGTIGVIDADEVDDSNLQRQVVHTIDRVGMPKVESARIAIEAINPD
                     VTVITHQMRLTKENALEIFAGYDVIVDGSDNFSTRYLVNDACVILKKPQVHGSIFRFD
                     GQATTFDPRQEESPCYRCLFPSPPPPELAPSCAEAGVLGVLPGTIGMIQATEAAKLVL
                     GLGETLVGRLLMYDALQMSFRTLTIRRDPACPMCGPTGPDSMADIEYTDVSCAIPTVA
                     TVAR"
     gene            10033..11148
                     /gene="msrA"
                     /locus_tag="VGR29_02965"
     CDS             10033..11148
                     /gene="msrA"
                     /locus_tag="VGR29_02965"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEV2072580.1"
                     /translation="MVFSNLFGKKQEMPSREDAIPGRDAPVFEIPKTHAVNGNRIQPP
                     FPEGMEVATFALGCFWGAERLFWEMDGVWSTFVGYSGGYTPHPTYQEVCTGRTGHAEV
                     VEVIFDPQKVTYAQLVKVFFEAHDPTQGMRQGSDVGTQYRSAIYYHNDEQKKIAEELK
                     ANYDRALRKAGYPPVSTQVEQAGPFYYAEDYHQQYLHKVPGGYCGLSGTGVTCAIPSQ
                     SPVSIEEPAPVSGGDDVAAADPGLKPLPETEDEWRQLLGDQQYKVLRQAGTERPFTGE
                     YNAVWQEGTYRCAACGNPLFDSSTKFDHGCGWPSFGEALPDAVEYIEDRSHGMVRTEV
                     RCARCHSHLGHVFNDGPREYDGARFCMNSVAIDLQKR"
     gene            complement(11208..13181)
                     /locus_tag="VGR29_02970"
     CDS             complement(11208..13181)
                     /locus_tag="VGR29_02970"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEV2072581.1"
                     /translation="MSTKQNRDRHEDVISSEAFAFYDPPAYRDAWSNRARTSGDDVLR
                     SSLDRTRRYPRLVSRRSLIAAGVAALGATAGIAVPLTTRGSFYPGTSVGGVDISEMSM
                     ESARALLQEQFAPFEELAVNYVFENQQWDAGLADLGFAIDYEATLNNAYNHGRDGVVD
                     RYSGVLLSTDNQSFPVVFVLDNEQLTAFLGQIGPEIKGAVRDAQLYLSGDEIKILKNR
                     DGRQLDIERAVQDTVAAVQSARRSTVSLTTVPVVSPITVADLEPVRVQTEILISDGVT
                     VQYGNTRWNVPREMLIESLTFPDEGDPTAPWLDAGMIAAGLQGIADEMYAAPVNAVVG
                     WDSGVYAVEDDIPGQEVDMEQLTAGLIAAAATADARLVELPMRELLADVRTDNLGELG
                     ITDFLAEGSSSFAGSSEARAENVRVSAGHVAHTLIAPGETCSFNSVLGPISLDNGFVE
                     GKIIKGTWIESDIGGGACQASTTVFRAALFAGLEFGEWNHHTFRLAFYEADGSPPGLD
                     AAIYQPNNEWEWELDLTFVNPTDSWMLLEMSAEGATAVASLYGSPTGYDVDVSVPYVS
                     APIPPGPPMEKVDDKLQKGQREKIQSAGDGFDVMMRRVVSREGEVIEDKEFWSKYQPQ
                     QETWAIGPGTKRKFPVDGESPPTETEEATEAVT"
     gene            13498..14478
                     /locus_tag="VGR29_02975"
     CDS             13498..14478
                     /locus_tag="VGR29_02975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2072582.1"
                     /translation="MTVRFGVFVPQGWRMDLTAIEDPVEQFEAMASVAREADKGPWDS
                     IWVYDHFHTTPEPTMNTVFESWTISATLARDTERVNIGQMVNCNGYRNPALFAKIAST
                     VDVASHGRLYAGFGAGWYEHEWRAYGYGFPETKERMAAFREGVQILHKMWTEDKPVFE
                     GKYYTIDEPINEPKSHKKGYKVPLWIGGGGPQVTLKLVAQYGDACNVGGGNPATCREK
                     LEILQGHCEKVGRDYGEITKSTSLNVYPIAKGADPEQATAKVRANYDNVSFEKFSEGT
                     IVGDADVIGEKVQALVDVGIDYVINYIPGVAYDTEAMQVYANEVIPSFAK"
     gene            14568..16151
                     /locus_tag="VGR29_02980"
     CDS             14568..16151
                     /locus_tag="VGR29_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEV2072583.1"
                     /translation="MSEYSSQLGAERPAGSAVRRYHETTNHLIDTTRLGEQYAGSMDE
                     ALRPRLYKLYPTLSPIPLPQDLQPSTTQALDAIADATGAGAPGEQIPDLSTLARLLLL
                     TDGVIRRVNRGGREVEFRAAPCTGALYHEEIYLVCGDLPGLPAGVYHYGVHDHALRRL
                     RDGDYRAHLVEASGGEPALVHAPAILVTTSVFWRNAWKYLNRAYRHTYWDTGTMLPNT
                     LAAAATAGLPARLVLAFADELVATLLGVDLDQEGVISLVALGRTTNEPPPAPPIDALA
                     LPTEPYSVRELDLPLIRETHRATLLRTGEAAAQWRSIDSDTESSPAPASSLIALPAPG
                     PMSLPRDPFEDLIRRRRSTRRFSHEPVTQDQLSTLLDRTTRGVPSDSLGPEGIPFNTA
                     YLIVNAVEGLTPGAYVYHREAHALELLHSLTEDETRERSSALALGQDAGGDAAVNIYF
                     LSDLDLLLERYGDRGYRLAHLGGALVAGKLYVIAYALGLGSTGLTFFDDAVIDFFAPH
                     AAGKSVMFLVAIGVPRRKT"
     gene            complement(16568..17359)
                     /locus_tag="VGR29_02985"
     CDS             complement(16568..17359)
                     /locus_tag="VGR29_02985"
                     /inference="COORDINATES: protein motif:HMM:NF004203.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2072584.1"
                     /translation="MTNAGQFAGKVALVTGGGSGIGRATAIELGRQGALVAVLSRQQA
                     RIDETCAEVTAAGGQAIGISADVTSEDELTGAVRQIQDTWGRLDLVVANAGANGVFAP
                     LEDLTLDEWNQTMATNLTSTFLTTKVSLPLLKRQGGSIVVVSSVNGSRNFNYPGAVAY
                     STSKAGQVAFTKMVALELAQHKIRINVVCPGAIRTNIEEATHPRNIDQIRWPVEFPKG
                     VSPLAGGPADPTQVARVIAFLLSDAADHVSGTEMWVDAAESLLVG"
     gene            complement(17909..18712)
                     /locus_tag="VGR29_02990"
     CDS             complement(17909..18712)
                     /locus_tag="VGR29_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2072585.1"
                     /translation="MTAFILLNRRLARRIERNLGHPEGHEQNPLLYLYINVVAELGNA
                     KANQVIVDLGAGKQCLFAALLEPETHSRIIGVDISAEAMASNEGLSEMVVTNAEEGLP
                     LRDAEADLVVSMSTLEHLRQVQPVARDIYRVLKPGGHTIHVFPSRNAPFSLLNRFLPH
                     WITRKLLRALIPGSEGIQGFPAYYDHCTPREMEELLSRSGFNVVRTEWSTYQSDYYGF
                     FIPLYLVSLAYDLIVQTLNIRRLAATVMIVAQKPELDLQEGQYRHVSNA"
     gene            19302..20927
                     /locus_tag="VGR29_02995"
     CDS             19302..20927
                     /locus_tag="VGR29_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072586.1"
                     /translation="MSSHLDPAVTNAEPAHALEAPQPDTVLPASSRLPRSTQRERLVQ
                     TFHAASVQQRFLALLLVIFLFKGIIIAFVFPPYTGHDEVAHYAYLEILAEQGRVPLIP
                     DLEEWRNQYERPGYQPSFDRMSEEFYFPYARYTTPDWFSGYQIVAYAVTFEGEPYPSG
                     WVYTGNHPPLFYLLLTPLFWLLADQTIETQLYVFRLLTIPFGMLTVLFAYLTVRTIFP
                     RDRFLAMTVPAFVAFQPQIAYEAAMLNNDILAIMFTSAVIWLIAVGLRRRFPLWTCLL
                     IGLCFGLAMLSKSTSVTSAAVIAFAMVLGLGIRNWREWLPRGVVTAGIAGLLVWPWLF
                     YMWSTYGDLSALERIETLQWWNYGGGEGRSIWSMLSDPDFFWERWRETWGAFGWRLIE
                     LDTVVLRWILGVTLFATVGLVVYVLRFFLTRRGIDNAEGAGQDRGVARDQADPTLAIM
                     PWQVTAVVTMGVTCVLAYYAILQFGTTFSLTQARYYFPAIVPGAILFMLGVRSWFPRT
                     WLPYVGAVTFLGLVGLNLLIYTAYVIPYWNPGV"
     gene            complement(21121..>21400)
                     /locus_tag="VGR29_03000"
     CDS             complement(21121..>21400)
                     /locus_tag="VGR29_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2072587.1"
                     /translation="LRAGLRHSLSMLHLTMADKQLERLRDVKQPTLVAAGEHDPIVPP
                     RWVHEMAKRMPNACARILAGCPHAMTYSRPHALAGAIDAVIQEWIHET"
BASE COUNT         4002 a         6295 c         6802 g         4301 t
ORIGIN      
        1 ggacgaggat ggctcaggcg cgcactggct gttgggttcg acaccccctc acgaattcgg
       61 gcagagggtc tctatgcagc aggctctctc gccgcaaccc agggaaacga caggactgcg
      121 gagccgttgc tggaggcggc gatgtccgac tggcgagtgc ttggtgagcg attgggcatt
      181 gcacgcacgc tgcacacact tggaaccgtt gccgttgagc ggaacgacaa cgaacgagcc
      241 gaacacctga ttagccagtc gctggccgaa tatggtgctc ccgcaggctc aagtgatgcc
      301 cccatgggcc gccttggcat tcagccagct cgcgggtgcg gtttcccgtt tgggcgaccg
      361 gcagcgtgcc atggatctcg ggaagcaggg tctgctcaag cagcaggagg caggaagtgg
      421 ccccggtatg gcgctcgcgc ttgtgtacct cggcgatatt gcgcttgact ggggagacta
      481 cgctgaggcg catatccggt acagggaggg actggcacag atgtggcaga cgggggatcg
      541 atggcatctc ctgcatgcat tggctggctt ctcaatcatg aaggcacagg tcggccctgc
      601 cgaggctgcg gcccgactgt ttggtgccca ggcggctgct cgtgccgcca ctgggcacca
      661 gatccctccc cggtatcgag acagttttga tgcggctgtg gtgaagctac gagcaacgct
      721 tggtgaagaa gcgttttcga catcctggac agagggcatg gctctcgaac ttgaggaggc
      781 ggttgccgag gcgctggaga gctgagtcgc agaggctacg acggaacact tgctccggct
      841 caacaagcaa agtcgtgccc aagccgggaa gggcacggcg atctggcatc cggacaacct
      901 gccatgcacc acagaatccc ccctctggtc cgaagtatgt tctggaccag aggggggatt
      961 gcctccatgg tgaccgcctg gagtctcagg ccgggccaga gcgaaaggat gacagctggg
     1021 gcaatcagcg cgtagcgcca aaggcaggac gtccacattg tgaacaatac atacccctgc
     1081 caggatcaag cctattcgtg gcacgtgccg ccagtgatgc gcacggggcc gccctctgga
     1141 accgcttcac aagccgtatt ggtcccgcgg ctggcagcac tgaatgtgcc ttcgtccttg
     1201 ccgacgttat tgccatcttc gtcgaagagt cgcggcgtga tgtttcttgc gaggtccttg
     1261 ttgtcgacga cctccccggc aggtgtcgta atggtaatcc tgtgagctgc gacatcgccc
     1321 ccgctggcca gtgtcagcgc cagaacgaac acggcagcac aaaatgcacg tttcatggga
     1381 ctgctccttc acgtgtgtat ggaatgatgg gaccggtcct tatgtatcaa catacgcctg
     1441 tgcacctata atgtccaata cgtattcgtg atcatcatca tagggaaaca ctatgattga
     1501 gcggcccagc cttcaggcac tggcactctt cctggcagtg gtcgatcatg gcacgatgac
     1561 cgccgccgcc gaggcggagg gcatagcaca gccggcgata tcagtccacg tcaggaacct
     1621 ggaacgcttc tatggagtgc cgctgatgga gcgtagcggg cggcgggtac gaccgacggc
     1681 gaccggggaa ctggtggctt cctatgcacg tcgcattgcc ggtcttgtca acgacctcgg
     1741 acacgcggta gaggatctcg aagggttgcg aactggccga ctggtcgtcg gtgtgagtgc
     1801 gacagtcgca gaaacatggc tgccggggat gctcggccga ttccgccggg cgtacccggc
     1861 aatcgagatg gacgtgcgca tcggcaacag cgagcagatt ctccacgaag tgagcgagcg
     1921 cagtctggga ttcgggattg tcgggaggcc ggaccccggt cccgacctcg ttgcccgtcg
     1981 tctcttcagc gatggcctgg agttcttcgc cgccgcagga agctcccttc tcgcgcgtca
     2041 acccctgcgg ttggacgatc tggccgatga aacgttcgtt cttcgcgaga ccggctcggc
     2101 cacccgggag tacatgctcc gttgcctggc cgcgcatgac atgaacccag tgaagacgat
     2161 ccagctcggg agtaacgagg cagtgaagcg ggcggttgcc gcgggcctgg gagtaggaat
     2221 cctgtcgtcg cagacactgg atgtcgatgt tagagctggg gacgtggtga tcctccaatg
     2281 tgccggttgg gagtgtcgtc ggcatttctg gctggtgcag cgtcgtgatc gtgtgctgtc
     2341 cagggccgag caggccttcc tggatctgcg ctgacggtat atctcatatc cccggtgtat
     2401 gttccatcgc tacggcacaa aagacatcca tgtgtgtagg ggtcaatcct tcgtgacctc
     2461 ggccgagcca ctacggatgc tccgggacat tcctgatacg ccgaagaaag tgacatcaaa
     2521 ggccgccggt gctaaggttg caacaggaat gagggcaatc agccttaagt aggcgtggga
     2581 acgccatggg gacacgagag agctcaccat tcggaagcct gttgcggcat taccgcctgg
     2641 cagccgggtt atcacaggag acgcttgccg agctgactgg attaagtact cgggggatca
     2701 gcgatctgga gcgcggggca cgtacagctc cccggttgga aacgctgcgc ctcctcgccg
     2761 ccagtctcgg cgtggagggg cctgggctgg ccgccttcct tgccgcccgc agcgctgtta
     2821 cggctgaagc agttcccact tctcaggcgg accatgcgat gcctccgctg gcactgacgc
     2881 cgctcgttgg ccgggagcgc gagatccggg ctattaccga cctgttgtgt gacgatgacg
     2941 tgcgacttgt tacgctcacc ggtcctggtg gcgtgggaaa aacgcgtctg gcacaagctg
     3001 ttgcacatga ggtgatgaat cagttcgccg atggggtggt cttcgtggac ctgtccccgg
     3061 taagggatgc ccagctggta ttgccgacga tcgccgcggt gtttggggtg caggatgtcg
     3121 gcgcagctgg tgtgatggct gatgcgcttg tcacagcgct ggggcatcaa catgtgctac
     3181 tgatcctcga caacctagag caagccattg atgccgcacc tgatatcgcc atgatcgtat
     3241 ccaggtgccc ctggctcaaa gtcctggcta ccagccggat ccttctgcgc ctgaccgctg
     3301 agcaggtctt tccggtctca ccgcttcagc tccctgatgg agccaccaag ccttcgctac
     3361 ccgagcttgc acagactgag gcggttgcct tctttgtcaa tcgcgccagt gctgccgacc
     3421 tcacgtttca cctgaccgag gacaacagcg gacccgtagc cgaaatcgtc cggcgccttg
     3481 aggggctgcc actggcgatc gaattggctg ccgtttggac acggacgctt cctcctgagg
     3541 cgctgctagc gcgattggaa cggcggttgc cactgcttac cggcggttcg cgtgatgcgc
     3601 cgctgcgaca gcggacgatg cgggacgcca ttgcctggag ccacgatctc ctcacacccc
     3661 atgcgcaagc cctttttcgt cgcctcggag tctttgttgg cggcttcacg ttcgaggcgg
     3721 ctgaagcagt gacggggagg gatgacgagg gatccggggt tgatttcctg gatggaatcg
     3781 catcgcttat agaaagaagc ttgctacggc gggaaatggg tcccgacgct gagccgcgct
     3841 acgtcatgtt ggaaaccgta cgggaatttg ggctcgacca gcttgttgcc aggggtgaga
     3901 cgccggcggt gcgtgctgcc catgccacgt atttcctcca actcgctgag cagagcctcg
     3961 agtcattcct gggacctgaa caccgccgtt ggttggatcg ggtggacgct gaacagggga
     4021 acctgcgggc ggctgcaacg tggttcgagg aagccaacga gactgatcac ttgctgaggc
     4081 tcgctcgggc gctgggtcac ttctggtata gccgtggtca cttgaccgag ggccgttcgt
     4141 ggttggaccg ggcgctcagc ctggcgacga gtgcaccgct cgagctcggg gcggacggct
     4201 tggcttgggc gagtggcctt gcccaccgcc agctcgacat tgaagtcgcg gtttcactcg
     4261 ccgaggcaag cctggttgag tggcagaagc tggagggaac gccgcctggc cgggcacggg
     4321 ctgttctcac tcttgccatc gggctggatc atcggggcga taaagaccgc gcggccgaat
     4381 tttacgcaga ggcggcggag atgttccgtg ccgagggcca ggactcctgg gctggcattg
     4441 ctatcgtcaa tctcgcgctc gtcgaggggg gacgaggcag ggtgcagctc gcccgtgacc
     4501 tgggtgagca ggggttgtca ttggtccaat ccgcgagaag tgcgtggggc gttaatcacg
     4561 cgctggccgt actgggtgat ctggcgctgg atcgggggga tcatgacgag gccgtgcagc
     4621 ggtaccacca atgcctgacg gcgtccattg atgtgggcga ccccgtgcag atgggacacg
     4681 acctggtgcg gctgggcgtc gtggcagcca cattcggcga tatctcccgg gcggtccaac
     4741 tttttggggc agtggaggct ctgcgcgagg gtgctcatcg aacgctgttg ccgccgaacc
     4801 tccgtcccaa gtatgaggca gccttgctga aatcccggga gtccctgggg gaaaacgcct
     4861 tcaaagacct ctggagggct gggcgagggt ggtcgctggg tacggccttg accgaggccg
     4921 cggcagttac tctcggcgcc gttggaagca gccctccctc cacgggacag catccaccgc
     4981 aggcttataa cctgacggag cgtgagttgg atgtgctgcg tctgatggtg gcgggcaaga
     5041 ccgatcgcga aattgcggag gagctcttca tcagccgccg caccgtcacg acacacgttg
     5101 gcagccttct cctcaagttg ggggtcacga accgcgtcca cgccgccact gttgcgatcc
     5161 aggacgggct cctttaagcg atcctccgcc tgatttctca agaatgttgg cagtgagcat
     5221 tacccacgca aaacatacgc atccgggcat cgctggtaca gcaaaaatac atgacagcag
     5281 ggatggctgg tgctatgctg aaagtctagg gactaccaaa acgacctcgg gagtagtagg
     5341 cgtgggaacg ccatggatgc gaagcaacct tcagcattcg ggaggctgtt gcgacagtac
     5401 cgtctggtgg cggggttatc gcagagaagc ttgccgagcg cgccgggcta agcacgcgca
     5461 ggatcagcga tctggagcgc ggggcccgta cgactcccag gctggaaacg gtgggcctgt
     5521 tagctgacgg cctggagcta ggcccttccc aacgtagcgc tttcctgggc gctgcacgtc
     5581 cgtcaaggga ttcccacctg cctgaaccta cccagatgtc ccttctgccg cgcgccatta
     5641 cgccgctcat cggacgagag cgtgaactcc aggtggtaaa ggagtttctg cagcgcgatg
     5701 atgtcgcgtt ggtgacgctg gccggtactg gtggggtcgg caagacgagt ctggcactgt
     5761 cagtggcggc agatctcgtg gacgatttcg acgatggagt cacatttgtc tctctcgctc
     5821 ccctcaacga tccggcactg gtcgcctcta cccttgcccg ggcactcggt gtgagggagg
     5881 ctggagggcg atcagttgtc gaagcgctcc gttcgttttt ggccttgagg cgtcaactcc
     5941 tgatcctgga taacttcgag cacgtcctgg ctgctgcacc tcttgtcacc gatctccttc
     6001 aattctgttt gggaatcaag gtgttggcaa ccagccggga gcggttgcat cttcgcggcg
     6061 agcaggtgtt cgaggtactg cctctcgggt tgccggatca acagcatctg ccctcactgg
     6121 aggtgttgag ccacgtgccg tcggtgcaac tctttgtcga acgagggcgg gaagtgcagc
     6181 cgcagttcac gctcgcagag aacaatacag cagaggtatc cgaaatttgc cgtcgccttg
     6241 acggcctgcc actggcgctg gagctcgccg cggcatggat ccatgtgttg ccgccggcat
     6301 ccctcctcac acgtcttgag cggcgtttgc ctctcctgac gcaaggccct cgcgacgtac
     6361 cgttgcggca acagacgctg cgtgacgcga tcggctggtc ctaccacctc ctgtccgggg
     6421 ccgagcagcg catgttcagg cgcctggctg ttttcgtggg cggatttgat cttccagcgg
     6481 cggaggcaat cgcgcagcga gtcgacggga tggagagcgt ctccattgtc acctcgctgg
     6541 tcgacaagag ccttctgcgg caagttgagg gcctggttgg tgaaccccgc tttggcatgc
     6601 tggagatggt gcgggagttc gggctggaac gattggtggc aagtggcgag gaatccgcta
     6661 ttcgtcagac acacgcctcg tacttcctgg ccttcgccga aaaccttcga ccgaagattg
     6721 agggccccga gggaccggtt gtattgacca gtctggagaa tgaacatgcc aatctgcggg
     6781 cggcactcga atgggagatc gagggcgccg acgccgacgg ggcattgcgc tttgtcgccg
     6841 caatgtggaa gttctggtgg gtccgccgcc atttgagcaa cggccgtgcc ttggccgagc
     6901 gagcacttgc acttccgggg agtgggccat cggcactgag ggcggaagcc tcgtatgggg
     6961 ccggatcact ggcgatgggg caggctgact atgcggctgc ccagcttcac ggcgaggcgg
     7021 gactggcctt ggcgagcgag atcgacgatc cattctggac cggaatgccc ctgaatttgc
     7081 tcggcaatgc tgctcgtctg cagggagatt acggaaaagc ccgcgcccgc tacgagccgg
     7141 ccttggcgct ctatcgcaat ttggggcgga cacgcccgtt cgcccggcac gccgccgcta
     7201 tggtgctcgt tagcctgggg actgtcgctt acgcagaggg cgacctcgaa gaagcgattg
     7261 tccgcaacca ggaggcgctg acaatctggc gagatcgcca ggatctgtgg ggacaaggta
     7321 ccgccctgat caaccttgga gcggtggcat cgaggcaggg cgaactctgc ctagcggagt
     7381 cgcactaccg ggagagcatt ctctgtaacc agacaaccgg cgatcgtgcg ggtatcgggg
     7441 aggggctggc cgggttggct ttggttgccg ctagtcgtgg gctatcggag cgggcggttc
     7501 gactgttcgc tgcggcggag gccatacggg aggtagccgg cactccaaac gctgcaatga
     7561 ttccggccga ccgcaaatgg gttatgacgg cagtgaagac acgtttggga gccgacgcgt
     7621 tcgcggccga atgggaaaca gggaaggcat tgaccgccga agacgcggtg gctgaagcga
     7681 tggagccacc cttaagcctg ccaggggagg cacccaaagc caaactcgga gatagtttgg
     7741 ggctcagcgg gcgtgagttg gaaatcctgc gcctcattgc cgagggacga tccaaccgcg
     7801 atatcgccgg ggtcctgttt atcagtcccc ggacagtcac cacccacacg actggtatct
     7861 ttaccaagct cgatgtgggt tcccgcacag ccgctgtggc ggtagcccgc gaacgtgggc
     7921 tcgtatagcg ccagtccggc attgatgcca ttgcgtggcc gttgcgtcgg tcccgccaac
     7981 tggaacggtt cccgaagcac gatatttgta cgatttgaaa tggatcccca cgcccgatga
     8041 tgacgatgac ccctcaatga tgcagggggc accccatagt cttgggatca aaaccctgag
     8101 gcacaacgaa gaagggcatt gtcatgtcta caccgaagcg tttggtcctt gcattgatcc
     8161 tggttgttct cttccttggt caagcagggg cacctgccca tcccgaccac gccagttgtc
     8221 aaccactcag tgatcagatc gcaaaggttg ccaaggctgg agcggcgggc gagttcatgc
     8281 ggacgctggc gttgaatcac caggtggctg agataggtgc tggcttcccc gtccagttct
     8341 gcgaaccaaa gccgtagctt ctcccaccaa cttgaaccaa gcaattgagc agcgtcttcc
     8401 gacgaatcgt gatggtccgc tgctcttgca aacggaaggg cgcacactgc tgatcactgt
     8461 ttgccactgt gatgatttag gtgatttata cgaagcccat ttttgtcctg gccattggta
     8521 ccaggtctgc agcacgcttc gactttgcgt cgatgagcaa ggtctttgtc gctctctgtg
     8581 taaatagagc aagcaactac ttgggcccag ttgaatcagc gaaaaagacg agcgccaggg
     8641 ctatcgtgcg accgtagcta cggtcgggat tgcacacgag acatcggtgt attcgatgtc
     8701 cgccatggag tcggggccgg ttggaccgca catcgggcag gcggggtctc gcctgatcgt
     8761 cagcgtccgg aagctcatct gcaacgcgtc gtacatcagc agccgtccca ccagcgtttc
     8821 cccgagaccg aggacgagct tggcggcctc cgtcgcctgg atcatcccga tcgtgcccgg
     8881 caggacgccg agcactcccg cctcggcgca gctaggcgcc agttccggag gaggaggaga
     8941 ggggaagagg cagcggtagc aggggctctc ctcctgtctt ggatcgaagg tcgtcgcctg
     9001 accgtcgaag cggaagatgc tgccatgcac ctgtggcttc ttcaggatca cgcaggcgtc
     9061 gttcaccagg taccgcgtcg agaagttgtc cgagccgtcc acaatcacgt cgtaaccagc
     9121 gaagatctcc agcgcgttct ccttggtgag ccgcatctga tgagtgatga cggtgacgtc
     9181 tgggttgatc gcctcgatcg cgatcctcgc cgattcgacc tttggcatcc cgacccggtc
     9241 aatcgtatgg acgacctgcc gctggaggtt gctgtcgtcg acctcgtcgg cgtcaatcac
     9301 gccgattgtg ccgaggccgg cggcggcaag gtagagtgcc gccggggaac cgagaccacc
     9361 cgctccgatc agcaacaccc tggcgtcaag cagttggagc tggccgcgct cgccgatctc
     9421 cgggatgagc acgtggcggc tatagcggcg tttctggtcc atggtcaacg tgcgcggctg
     9481 ggaccacgcc tcgccggcat ccttccaggc gttgaacccg ccggccagcg aggcgacgtt
     9541 cgtgtagccc aaatcctgca ggctcttcgc ggccaaggcc gaccgcgtgc caccggcgca
     9601 atagacgaca actggggccg tcttatccgg caccgcatcc tccacccgaa gctcgaggta
     9661 gccacggggg atggtgaccg cctcgggaac gatgccctgt tcggtttcgt ctggctcgcg
     9721 gacatcaatc acggcggcat gcccatcctc catcagggca cgtgcgccgg caacatcgac
     9781 ctcacggatc tcggcacgaa tctggtccag caatgcgcgg aatgtagtca tgggggagtg
     9841 caatcctctg tcagaacggc cggtcgtgcg agccggtgcg atggtgcttg tcttgcagga
     9901 tatcctatta agaggagcag attcctgctc cggcagcaat agccgggcaa gcaccgcaca
     9961 ttggcactga aacccggtaa aatggagatg cagggcagaa ccgtcctgcg gcaatccatc
    10021 ggagaacaca tcatggtatt cagcaacctg tttggcaaga agcaagagat gccgtcccgg
    10081 gaagatgcga ttcctggccg cgacgcgccg gtgttcgaga tcccgaagac gcacgccgtg
    10141 aatggcaacc gtatccagcc gccgtttccc gaggggatgg aggtcgcaac gtttgcgctc
    10201 ggctgcttct ggggcgccga gcggctcttc tgggagatgg acggagtctg gagcaccttc
    10261 gttggctaca gcggggggta cacaccccat ccaacctacc aggaagtctg caccgggcgg
    10321 acgggacacg ctgaagtcgt cgaggtcatc ttcgacccgc agaaggtgac gtatgcgcag
    10381 ctggtgaagg tcttctttga ggctcacgat ccgacgcagg gcatgcgcca gggcagcgac
    10441 gtcggtaccc agtaccgctc ggcgatctac tatcacaatg atgagcagaa gaagatcgcc
    10501 gaagaactga aggcgaacta cgatcgggcg ctgagaaagg ccggctatcc gccggtttcg
    10561 acccaggtcg agcaggcggg cccgttctac tatgccgagg actatcacca gcagtacctt
    10621 cacaaggtgc cggggggcta ctgtggcctg agcggcacgg gcgtcacctg tgccatcccg
    10681 agccagagtc ctgtctccat cgaggagccg gctccggtga gcggtggcga tgatgtcgca
    10741 gcagccgatc ccggcctgaa gccgctgccg gagacggaag acgagtggcg acagctgctg
    10801 ggtgaccagc agtacaaggt gctgcgccag gccggcacgg agcgcccgtt cacgggcgaa
    10861 tacaacgcgg tctggcagga gggcacgtat cgctgcgcgg cgtgtggtaa cccgctcttc
    10921 gacagcagta ccaagttcga tcacggttgc ggttggccga gctttggcga ggccctgccg
    10981 gatgctgtgg agtacatcga ggatcgctcg cacggcatgg tgcggacgga agttcgttgc
    11041 gcgcgttgcc acagccatct gggtcacgtc ttcaacgatg gcccgcgaga gtacgatggc
    11101 gcgcgattct gcatgaactc agtcgcgatc gacctgcaga aacgctagcg gacaaccaca
    11161 accgacgaag aacatggcaa cggcggggag tactccccgc cgttgcgcta tgtcaccgct
    11221 tcggtggcct cttccgtttc cgttggtgga gattcaccat cgaccgggaa tttgcgtttg
    11281 gtgcccgggc caattgccca ggtctcttgc tggggctggt acttgctcca gaattccttg
    11341 tcctcgatga cctcgccctc acggctcacc acacggcgca tcatgacatc gaagccatcg
    11401 ccggcactct ggatcttctc ccgctgtccc ttttggagct tgtcgtcgac cttctccatt
    11461 ggcgggccgg gcgggatcgg cgcgctcaca tacggcactg acacatcgac gtcataaccg
    11521 gtagggctgc catacaggga cgcgacggct gttgcgccct cagccgacat ctccagcagc
    11581 atccaggaat cggttggatt gacgaaggtg agatcaagct cccactccca ctcgttgttc
    11641 ggttgataga tcgccgcgtc caggccagga ggtgagccat cggcctcata gaaggcgagg
    11701 cggaaggtgt gatggttcca ctcgccaaat tcaagccctg caaagagtgc agcgcggaag
    11761 acggtcgtcg atgcctggca ggcgccgccg ccgatatcgc tctcgatcca ggtgcccttg
    11821 atgatcttgc cttcgacaaa gccgttgtcc agcgagatgg gacccagaac cgagttgaat
    11881 gagcatgttt ctcccggcgc gatcagggtg tgggcaacat ggcccgcgga gacacgcacg
    11941 ttctcggcgc gagcctcgga tgacccggca aagctcgacg atccctcggc aaggaagtcg
    12001 gtgatgccaa gctcaccaag attgtccgtt cgcacatccg ccaggagctc gcgcatcggc
    12061 agctcaacca agcgcgcatc ggcagtggcc gccgccgcga tgaggccggc tgtaagctgc
    12121 tccatgtcga cttcctgccc tgggatatcg tcttccacgg catagacacc actgtcccaa
    12181 ccaacaactg cgttgacggg cgcggcgtac atctcatccg cgatgccctg caggccggcg
    12241 gcgatcatcc cggcatcgag ccagggcgcg gttgggtccc cctcgtccgg gaaggtaagg
    12301 gactcgatca gcatctcgcg aggaacgttc catcgggtat tgccgtactg caccgtgacg
    12361 ccgtccgaga tcaggatttc agtttgcacc cgcacgggct ccaggtcggc gacggtgatc
    12421 ggtgacacga ccggaacagt cgtcaggctc acagtgctcc ggcgggcgga ttgcacagct
    12481 gcaacggtgt cctggacagc gcgctcgatg tcgagctgac gcccgtcgcg gttcttcagg
    12541 atcttgatct cgtctccgga gagatagagt tgcgcatccc gtacggcgcc cttgatctcg
    12601 gggccaatct gcccaagaaa tgcggtgagc tgctcgttat ctagcacgaa gacaacggga
    12661 aaggattggt tgtccgtgga gagaagcacg ccggagtatc ggtccaccac tccatcgcga
    12721 ccatggttgt aggcgttgtt cagggtagcc tcgtaatcga tggcaaaacc gagatcggcg
    12781 aggcccgcat cccactgctg gttctcgaag acgtagttga ccgccaactc ctcgaagggc
    12841 gcaaattgct cctggagcaa cgccctggca ctctccatcg acatttcgga gatgtccact
    12901 ccgcccacgg atgttcccgg atagaagctg ccgcgggtag tcaggggcac agcaatgcca
    12961 gcggtcgcgc ccagggcggc gacacccgct gcaatgagac tgcgtcggct caccagccgg
    13021 ggatagcgtc tcgtacggtc caaggaactc ctcaatacat cgtcaccgga tgttcgtgcc
    13081 cggttactcc acgcatcccg ataggcaggg gggtcgtaga aggcgaatgc ttcactggag
    13141 ataacgtcct catgccggtc gcggttttgt ttggtggaca taaggataca tgttccgcag
    13201 gaaggatcgc gggggaagaa tttcgtcttg ctggcgcccc gagcacccga taatcgcccg
    13261 ggtcaagtat agcgtcacgc accgcacgcg gaggtgtgaa cagcggatac gaatgtgcga
    13321 tagcaagcaa gagggtgacc acatccttca cgggcaggca atctgcccat aggatgggag
    13381 ccggtcacag gaacgatacg gtccggcagg atccaatcct gcagggccgg tatcgcgcga
    13441 ggtcgttaag tatggaagcg aagctacaac agccttcctt ccgggagttt gggaacgatg
    13501 acagtaaggt ttggtgtttt tgtgccgcag ggatggcgga tggatctgac ggcgatcgag
    13561 gatcccgtcg agcagttcga ggcaatggcc agtgttgcgc gggaggcgga caagggtccg
    13621 tgggactcca tctgggtcta tgaccatttc cacacgacgc ccgagccgac gatgaacacg
    13681 gtctttgagt cgtggacgat ctccgcgacg cttgcgcgtg acactgagcg ggtgaacatc
    13741 ggccagatgg tgaactgcaa cggctatcgc aatccggcgc tcttcgccaa gatcgcctcc
    13801 accgttgatg tcgccagcca tggccggctc tacgccggat tcggtgccgg ctggtacgag
    13861 cacgagtggc gggcctatgg ctatggcttc cccgagacga aggagcgcat ggccgcgttc
    13921 cgtgagggtg tccagatcct gcacaagatg tggaccgagg acaaacccgt cttcgagggc
    13981 aagtactaca cgatcgacga gccgatcaac gagccgaaga gccacaagaa gggctacaag
    14041 gtcccgctgt ggatcggtgg tggcggcccg caggtgacgc tgaagctcgt tgcccagtat
    14101 ggcgatgcct gcaatgtcgg tggtggcaac cccgcgactt gccgagagaa actcgagatc
    14161 ctccagggac actgcgagaa ggttggccgt gactacggag agatcaccaa gtcaacgagc
    14221 ctgaacgtct acccgatcgc gaagggtgcg gacccggagc aggcaacggc gaaggtccgt
    14281 gcaaattacg acaacgtctc gtttgagaaa ttctcggagg gcaccatcgt cggtgatgcg
    14341 gacgtgatcg gtgagaaggt ccaggcgctg gtggatgtgg ggatcgacta tgtcatcaac
    14401 tacatcccgg gtgtggccta cgataccgag gcaatgcagg tctacgccaa tgaggtgatc
    14461 ccgtcgtttg cgaagtagac ggcttcgcgc aggggacagc gtgactgacg gcatgtgacg
    14521 tctctccatc gccatgccgt cggtcttcgg cagagggagg tcaagcgatg tccgagtatt
    14581 catcacagct gggggctgaa cggcccgccg gatcggcagt gcgtcgctac catgaaacga
    14641 caaaccacct gatcgatacg acccggcttg gggagcaata cgctggctca atggatgagg
    14701 cgctacgtcc gcgcctctac aagctctacc cgacgctctc gccgatccct ctgccacagg
    14761 accttcagcc ttcgacgacg caggcactgg acgcaatcgc ggacgcaaca ggcgccggtg
    14821 caccaggcga gcagattcct gatctgtcga ccctggcacg gctgctcctg ctgacagacg
    14881 gcgttattcg ccgcgtgaac cggggcggac gggaggtcga gttccgcgcc gctccgtgca
    14941 ccggtgccct ctaccatgag gagatttatc tcgtctgcgg cgatctgccg ggcctgccgg
    15001 ctggtgttta ccattacggt gtgcacgatc acgccctgcg ccggctgcgc gacggcgact
    15061 atcgtgccca cctcgtcgag gcaagtggcg gggaaccggc gctcgtccat gccccggcca
    15121 tcctggtgac gacgagtgtc ttctggcgca acgcctggaa gtacctcaat cgcgcctacc
    15181 gccataccta ctgggacaca gggaccatgc tgcccaacac gctggcggcc gctgccacgg
    15241 caggtctgcc ggcgcggctt gtccttgcct tcgccgacga gctggtggca acgttgctcg
    15301 gcgtggatct cgatcaggag ggggtgatca gcctggtcgc actcggccgg acgaccaacg
    15361 agccaccacc cgcgccaccg atcgacgcgc tggccctgcc gacggagccc tactcggttc
    15421 gcgaactgga tttgccattg atccgcgaga cccaccgggc gacgctactc cggactggag
    15481 aggcggccgc ccagtggcgg tcaattgaca gtgatacgga atcaagcccg gccccagcct
    15541 cctcgctgat tgccctgcct gcaccaggcc cgatgtcgct tccgcgcgat ccgtttgagg
    15601 atttgatccg gagacgtcgt tcgacccgtc gcttctccca cgaaccggtc acgcaggatc
    15661 agctctcaac tcttctcgat cgcacaaccc gcggcgtgcc gtccgacagc ctcggcccgg
    15721 agggtatccc cttcaacacg gcctacctga tcgtgaacgc ggtcgagggg ctcacacccg
    15781 gtgcctacgt ctaccaccgg gaggcgcatg ccctggagct gctgcactcg ctgacggagg
    15841 acgagacccg agagcgctcc tccgcgctgg cattgggaca ggatgccggt ggtgacgcgg
    15901 cggtgaatat ctacttcctc tccgatctcg acctactcct ggagcgctac ggcgaccgcg
    15961 gttaccggct cgctcacctc ggtggtgcgc tggttgccgg caagctctac gtgatcgcct
    16021 atgccctcgg gctcgggtcc acagggctga cgttcttcga cgacgccgtg attgacttct
    16081 tcgctccgca cgccgccgga aagagcgtca tgttcttggt cgcgattgga gtaccaaggc
    16141 gcaaaaccta agctactctg gtgaatgatt gcgatgtctg cctgagtacc ggcgctaccc
    16201 acgccgtgag gcacgctggg ggacgggtgg cgatgtgcac catccaaata ggggagcatt
    16261 tcgggtatcc ccttcagcga tctgtcatga gatcaatacc tcaggtccgc caggcggagt
    16321 agcggaggac ttgcggcgtg ggcacgaatg tggagggcac cgaacgccgc aatgtcctcc
    16381 tgaaaggctg cacggcgcga cagtgccgcg acaaaggctc gctgacgctt ctcgccctcc
    16441 gggctcggcc gaaggggccg ctctcgctca attgctccgc aattgcctat gcgtcgttgg
    16501 ttacggacat tcaacctctc tgaaaacatc tctcaagctt gagggtccgg ttgggtaggg
    16561 gtgagcgcta cccgacgagc aggctctcgg cggcatcgac ccacatctcg gttccactca
    16621 cgtgatcggc ggcatcggac agcaggaagg cgatcacccg tgcaacctgg gtggggtcgg
    16681 caggcccgcc cgcgagcggc gagacgcctt tagggaactc gaccggccag cggatctggt
    16741 cgatgtttcg tgggtgcgtt gcctcttcta tattggtcct gatcgcgccg gggcaaacga
    16801 cattgatgcg gatcttgtgc tgggcaagtt ccagcgccac catcttggtg aatgccacct
    16861 gcccggcctt gctcgtggag tacgcgaccg cgccgggata gttgaagttg cgcgacccat
    16921 tcacggagga aacaaccacg atcgacccac cttgtcgctt cagaagcggc aggctcacct
    16981 tggtggtgag aaacgtactt gtcaggttcg tcgccatcgt ctggttccac tcatcgaggg
    17041 tcaggtcctc gagcggcgcg aagacaccgt tggcgcccgc attggcgacg accagatcaa
    17101 gccggcccca tgtgtcctgg atttggcgga cggcccctgt caattcgtct tcgctggtaa
    17161 catcggcgct gatcccgatg gcttgacctc cagctgcggt gacctccgcg caggtctcat
    17221 caatccgggc ctgttgccgg gaaaggactg cgaccaatgc gccttgccga ccaagctcga
    17281 ttgccgtcgc ccggcctatc ccggaaccac caccggtgac gagtgctacc ttgcctgcaa
    17341 actgtccagc gttggtcacg tattgatcct tcttcatgag ttcggtgtgt catgtttggc
    17401 caagtctcgt ccatggagga tgcgaaagaa agccagttct cagtgaggtg tattcatttc
    17461 gttggcgggg cctgtaatgc caaggggctt tttcaagtcg gaccgtactc ctcatcaccg
    17521 tattcctaaa tcggcgatgt gatcgcccca gatcccgctg gtgccgtggt cgtcaaccac
    17581 gggcattgcg aggaggggca tgacgcggtt tggggctgga aacgaacatt tgtgatattg
    17641 cgccgcacag acaggcgcag ggctggtcct cggcagcagg tgtggttgcc gggtctggat
    17701 cactattggg gataatcctg ctgaatcagc gcaatgatcg acactgccag ggctcgcgag
    17761 gcgtgtcagt ggggagaccg tcagtatgcc aggagagcac gcgtcaggtt gatagtcatc
    17821 gccatgtgca atgtggctgc gcgggtgaat ggccgaagaa cgcggcctgg agttacctgt
    17881 tttcgtatcg gacaggaacg ttctctctct aggcgttcga gacgtgccta tactgccctt
    17941 cttgcaggtc gagttctggt ttttgagcaa cgatcataac agtcgcggca agtcgcctga
    18001 tgttcagagt ctgcacgatc aagtcatacg cgaggctcac aagatagaga gggatgaaaa
    18061 agccatagta atccgactga taggttgacc attcagtccg cacgacattg aagccgctac
    18121 gcgaaagcag ctcctccatc tctcgaggag tgcagtgatc atagtaagca ggaaaccctt
    18181 gtataccctc actacctggt atcagggccc gcagaagttt tcgagtgatc caatgcggga
    18241 gaaacctgtt gagaagagag aatggggcgt tccgggatgg gaatacatgt atggtatgcc
    18301 ctccaggttt taggacacgg tatatgtcac gagccacggg ttgaacttgt cttagatgct
    18361 ccagcgtcga cattgagacg accaggtcgg cttccgcatc ccgaagtggc agcccttctt
    18421 ccgcatttgt taccaccatt tcactgaggc cttcgttcga cgccatcgct tcagcggata
    18481 tgtcgacacc gataatcctg ctatgggttt cgggttccag cagcgccgcg aagaggcatt
    18541 gctttcctgc tcctaggtcg acgatcactt gattggcctt cgcattgcca agctcagcga
    18601 ccacgttgat atagaggtag agcagggggt tctgttcgtg gccttctgga tggcccagat
    18661 tgcgctcgat gcggcgtgcc agtcgtctgt tgagaagaat gaacgccgtc accacctcgc
    18721 cgagtgatcg caatgtcctc atccccgctc gctcggccta gcttggctga acgttcgtta
    18781 agctgctgat agtggcattg tacgtccctg tagcagttca tcaatcaaga acgggctgca
    18841 ttgagcagcc gcccatatcc ggcaaagcgc cggagaggat cagggctcac tgggatccca
    18901 gtgaccagtc gagatagtgg gaggcaagca gaggttgctc agggtgcgac ttgctgaccc
    18961 ccttgactcc ggacagtgtc acaggatgca ttcggtaatg tgatgctgta cttccatacc
    19021 gcggcgttgc tggtgcttgg cctctcgaga tgtcgcacgt gcgtacctgt atcatcttca
    19081 atcaaggtat gtgtctcgga cgatcaacct ggtcaaccgc atacggagag catgttcctg
    19141 cacgactgga cacgttggtt tgcaatgagg ctctgctgga ctacaggaac gcgccggtca
    19201 cgtcgtatgc ggagctcgta agcccttaca cggaacgagt aagtctcggg atactgtcaa
    19261 gatatctgtg tcgccattga ttgaacgggg agttgccgga cgtgtcttca catctggatc
    19321 cggccgttac caatgccgag ccggctcacg ccctggaagc accccaacct gacactgtcc
    19381 tcccagcatc ttctcgcctc cccaggtcga cgcaacgtga acgcctggtg cagacattcc
    19441 atgccgcatc agtgcaacag cgttttcttg cactgctgtt ggtcatcttc ctgttcaagg
    19501 gcatcatcat cgcgtttgtt tttcctccct ataccgggca tgacgaggtc gcgcactacg
    19561 catatcttga gattcttgcc gagcaggggc gtgtcccgct catcccggat ctggaggagt
    19621 ggcgtaatca gtatgaaaga cctggctacc aaccatcatt cgaccggatg tcagaagagt
    19681 tttattttcc gtacgctcga tacactacgc ctgactggtt ttccggctac cagatagtgg
    19741 cgtacgcggt tacatttgag ggtgagccat atccatccgg gtgggtgtat acggggaatc
    19801 atccgccgct cttttatctg ttgctgaccc cgctcttctg gctgctggcc gaccagacga
    19861 tcgagaccca actctatgtg ttccggctgc tcaccattcc ctttggcatg ctgacagtgc
    19921 tgttcgccta cctgacggtg cgcacgatct ttccccgcga tcgctttctg gcgatgaccg
    19981 tgccggcctt tgtcgccttc cagccccaga tcgcctacga agccgccatg ctcaacaacg
    20041 acatcctggc catcatgttc accagcgccg tcatctggct gatcgccgtc gggctgcggc
    20101 gacgcttccc gctctggacc tgcctgctga tcgggctctg cttcgggctg gcgatgcttt
    20161 ccaaaagcac ctcggtcacc tcggcggcgg tgattgcgtt tgccatggtg ctggggctgg
    20221 ggatccggaa ctggcgggag tggctcccca ggggggtggt gacggccggg atcgccgggt
    20281 tgctggtgtg gccatggctc ttctacatgt ggtcgacgta tggggatctg tcggcgctgg
    20341 aacggatcga gacgttgcag tggtggaatt acgggggagg cgaggggcgc tcgatctggt
    20401 cgatgctctc agacccggac ttcttctggg agcgctggcg agagacctgg ggtgccttcg
    20461 gctggcgcct gatcgaactt gataccgtgg tgctgcggtg gattctgggc gtaaccctgt
    20521 ttgcgacggt cggactcgtc gtctatgtgc ttcgattctt tctgacccgc cgtgggattg
    20581 ataacgctga aggtgcaggg caggatagag gggtagcccg ggaccaggcc gaccctacat
    20641 tggcgatcat gccgtggcag gtgacggcgg tggtaacgat gggggtgacc tgtgtgctgg
    20701 cgtactatgc gatcctgcag ttcgggacga cgttctcgct gacccaggcc cggtactact
    20761 tcccggccat cgtgccgggg gcaatcctgt tcatgctcgg ggtgcggtcc tggtttcccc
    20821 gcacctggct gccttatgtg ggggcggtca ccttcctggg cctggtcggg ctgaacctgc
    20881 tcatctacac cgcctacgtc attccctact ggaatcccgg cgtctagcat agttcccgat
    20941 atgccgttcg ggcatcgagc caatgggctg ctcccgctcg attgctgcgc aatcgcctaa
    21001 tccacccttc ggggtacgcc ccctacacgg tcatggcggg tcgttcagta gggtaggggt
    21061 cggccctgcg tctgccggag tggatattgg ctcgccgaat gggtcgaaga cctgcccgat
    21121 ctacgtctcg tggatccatt cctggatcac ggcgtcgatc gcgccagcga gggcatgtgg
    21181 acggctgtag gtcatggcgt ggggacaacc agccaggatc cgggcgcagg cattcggcat
    21241 tcgcttcgcc atctcgtgca cccaccgcgg cggcacgata gggtcgtgct cgccagcggc
    21301 aaccagggtg ggctgtttta catcacgaag ccgctcaagt tgcttgtctg ccatggttaa
    21361 atgcagcatc gacagtgaat gtcggagccc ggcacgcagg
//
LOCUS       DASYWM010000074         8423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_190321, whole genome shotgun sequence.
ACCESSION   DASYWM010000074 DASYWM010000000
VERSION     DASYWM010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190321"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VGR29_03005"
     CDS             <1..360
                     /locus_tag="VGR29_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEV2072588.1"
                     /translation="GLGSYGGPNGDVYLMISVRDDPNFERIGDDIKTTVRVPLLTAVL
                     GGETVVPTLDGRVALTIPPGTQNEKVFRLRGKGLPKSGKGASGQGDLLATVHVDLPTD
                     LSEEETKLFKQLRNLRQ"
     gene            439..1797
                     /locus_tag="VGR29_03010"
     CDS             439..1797
                     /locus_tag="VGR29_03010"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV2072589.1"
                     /translation="MVTTRGAVAGATRPLACLYSSPEKGHIMPATVIMGGQWGDEGKG
                     KLTDALASGAAMVVRANGGSNAGHTVKTDQGTFKLHLVPSGILNGNVICVIGAGVVIE
                     LSTLIGELDDLADRGVDTSNLRISDRAHLVLPYHPEIDRLEEARRESDLIGTTLRGNG
                     PAYADKVSRHGLRVCDLLDPSILRRKLAIEVDAKNEVITRVYGSEPIDFDELYQKLTQ
                     SAERLIPFIAPTEVLVQDTLHANKDVIVECAQGAMLDIDYGTYPFVTSSSPTAAGACQ
                     GAGVAPTQVERVIGVYKAYSTRVGAGPMPTELNDQTGERIRRRGNEYGTTTGRPRRTG
                     WFDGVAARQTSRLSGVTELAVTLLDVLDMFDEIRFCSGYRFAGDELTHVPASLERLEA
                     AAATWETLPGWNRDTTSARHYGDLPENAQAYLARIEEQLGAPARYIGVGPAREQLIDR
                     LV"
     gene            complement(1881..2924)
                     /locus_tag="VGR29_03015"
     CDS             complement(1881..2924)
                     /locus_tag="VGR29_03015"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV2072590.1"
                     /translation="MNNRTQHAQHPRVAVLGGGAWGTTLALVSLRAGNGTALYVRDEA
                     ACRVLTEERRHPHSLRGVTIPESLSVSTDIEAVLARADAVILAIPTQKLRGALVPVRD
                     LFLGKIVVSAAKGIEIGTLLRPSAIVEEVLSDETSPVCALSGPNLSSEVAAGKPATTV
                     VASRNVEAASWIQSALIGAAFRIYTSIDIVGVETGGSLKNIIAIGAGIGDGLGAGDNA
                     KAAFLTRGIAEIARLGIAHGAQPLTFAGLSGIGDLIATCSSPQSRNHQVGTGLATGAS
                     LEEVLSGMSEVAEGVDTTRAAVELAHRTGVEMPITEQMYKVLFEGKSAAEAVRDLMSR
                     EPKEEIHGRIESF"
     gene            3032..3487
                     /gene="fabZ"
                     /locus_tag="VGR29_03020"
     CDS             3032..3487
                     /gene="fabZ"
                     /locus_tag="VGR29_03020"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV2072591.1"
                     /translation="MTLQMPLDTVAIRKILPHRYPFLLVDRIIEMTPGERAVGIKNVT
                     SNEEFFEGHFPEYPVMPGVLIVEAMAQVGGVALLSLEEHAGKMALFAGINNVRFKRQV
                     KPGDTILMEVALGTFRRGIGTGTATATVDGEVACRGELMFALVPDQTKG"
     gene            3496..4608
                     /locus_tag="VGR29_03025"
     CDS             3496..4608
                     /locus_tag="VGR29_03025"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV2072592.1"
                     /translation="MSPDPSDAIIGIDASRMHVRERTGTENYSDQIIRALLAADADWR
                     WRLYVNGEVAPPSLATPTNAEVRPLQARRLWTHYRLSREMLTRRPNLLFVPAHVVPIV
                     HPRTVVTIHDLGYLHVPEAHPVTQRKMLDLTTRWSARVAHHIIVPSAQTREDLIQRYG
                     VLAGKISVVHHGVDPQFRKVDRSNEQELRTRYNLYRPYVLAVGTIQPRKNLAILARAM
                     ETGAPDHDLVIAGKRGWMSDQVLSELRETSLGARLRIIDYVPDRDLPLLYASASIFVQ
                     PSRFEGFGMPVLEAMAAGTPVICASGSSLTEIAGDAAEFFPASDHALLAHHLVEILQD
                     PSRMAGMRDRGAAWSARFTWERAARETRRIFEEALT"
     gene            4643..5164
                     /locus_tag="VGR29_03030"
     CDS             4643..5164
                     /locus_tag="VGR29_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072593.1"
                     /translation="MNSSGRTIGIVLLAAGVIIGAALAAWLFFGVQEGNLRASGAAFG
                     AVLFFSVLVLPLVGAGIFFIVKGSAEARNLARVQEQRRLLDMVKTRGQLQISDLVLEL
                     KSSTDKVKNDIYDLVGRGLFSGYVDWDKGVLYSVEASKLQGRETCPNCGGQVELAGKG
                     MIKCPYCGAEIFL"
     gene            5209..6180
                     /locus_tag="VGR29_03035"
     CDS             5209..6180
                     /locus_tag="VGR29_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072594.1"
                     /translation="MDRLRKLGNVENRITELLHKVPGYNGYRDKENRRDEDKRLRESI
                     ANDVQNSANRLTQYNADLSAAREFTHLSRVEALIGQIRLLADRVRTASYGYGGIFTER
                     EVDAAALDQLRQFDLALQREVSSLGEKVQALTNSTPPRDDDVRSLSGEIDRLQLLFDS
                     RTEVVDQARPTRDAKVLELLETNEPAAPSPLLSVSKGDTLSVLGDNFIANATISIATD
                     QGPLQLIRVSEEEAGATWLLGSGVEGIVSARLIESLGEMPPYQTMQRAAATLDTENGR
                     QEGVTVQYAARMTGEGELELTVVFGDSARVYRGNEIRDIDVEVYGAA"
     gene            6219..6920
                     /locus_tag="VGR29_03040"
     CDS             6219..6920
                     /locus_tag="VGR29_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEV2072595.1"
                     /translation="MSILDRMQRLVRANVNDLIDRAEDPEKMIDQILRDMESNILTAR
                     SQVAAMIAQEKELEADLDETKQLAAEWGDKAKRAVDAGKDDLAREALRRKRDNEENAR
                     VYQQQHETQLQTVSNLKHQLQALESKYQQTQSQRDSLIARQRRAKASQQVAEFSSTLT
                     PFDASSELERMERKIRSTESQAVALAEMESTSLDAQFRELDYDVDIEQELEALKGGSS
                     ETAGQLEAGGQQSGA"
     gene            7029..8093
                     /locus_tag="VGR29_03045"
     CDS             7029..8093
                     /locus_tag="VGR29_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV2072596.1"
                     /translation="MAILDLIEFPNEATGDIVRRVPEGTSGEFRLGSQLVVRESQRAV
                     FFRDGKALDVFGPGRHTLSTNNIPLLTGLLGIPFGGDSPFRAEVYFVSMREFTDMKWG
                     SSQPIVFRDTELGMIRLRAFGTYSMRVSDPQLLVSQIVGARGSYTTSSIEDFLRSVIL
                     QEFTDLLADVQTSILDLASMAKEVADGTRAALADDFSRYGFELTSFQVQAITPPEEVQ
                     KRIDQRSGMAALGDMRTYTQFRTAEAIGDAANNPGSGGEGISAGMGMGAGMGMGAAMA
                     GAMRDAFRDDQQQPVDRNQRQGTGIQTTTCSACKATIPANSKFCPECGTNQQSVTCPK
                     CQTENQPGAKFCVNCGEKLA"
BASE COUNT         1754 a         2564 c         2516 g         1589 t
ORIGIN      
        1 ggcctgggga gctacggcgg gccaaacgga gatgtctacc tcatgatttc ggtgcgcgac
       61 gatcccaact tcgaacgcat tggcgacgac atcaagacaa ccgttcgcgt tcctctcttg
      121 actgctgttc tcggcggcga aaccgtcgtg ccaaccttgg acgggcgcgt cgctctgacc
      181 ataccccctg gaacccagaa cgagaaagta ttccggctca gaggcaaggg acttcccaag
      241 tccggcaagg gcgcttcagg acagggagat ctcctggcaa ccgtccatgt ggacctgccg
      301 accgatctca gtgaggaaga aacgaaactc ttcaagcagc tcaggaatct gcgacaatag
      361 attagtgtag gcaaaggtgg cgagcgccag ggcgcgcaca gatcgcataa gcgtccacgg
      421 ccagggagac ctgggacatt ggtaacaaca agaggcgcag tggccggggc gacccggcca
      481 ttggcatgtc tctactcatc accggagaaa gggcacatta tgccggcgac cgtcatcatg
      541 ggtggacaat ggggagacga gggcaaaggg aagctgaccg atgcgctggc ctccggtgcg
      601 gcaatggtgg tgcgcgcaaa tggcggtagc aacgctgggc ataccgtcaa gaccgaccag
      661 ggcaccttca agttgcacct tgttccctcc ggcattctca acggcaatgt catctgtgtc
      721 atcggcgcgg gcgtagtcat cgagctcagc acgctgatcg gcgagctcga cgatcttgcc
      781 gaccggggag tagacacgtc caacctccgt atctccgacc gtgcgcatct tgtcctcccc
      841 tatcatccgg agatcgatcg tctcgaggaa gcacggcggg agtcggatct catcggcacc
      901 accctgcgcg gcaatggacc cgcctatgcc gacaaggtat cccgccacgg cctgcgcgtc
      961 tgtgatcttc tcgatccctc gatcctgcgc cgtaagctgg cgatcgaggt cgacgccaag
     1021 aacgaggtca ttaccagggt ctacggatcc gagccaattg acttcgatga actctaccag
     1081 aagctcacgc agtcagctga gaggctcatt ccgttcatcg cacccacgga ggtcctcgtc
     1141 caggacacct tgcacgccaa caaggacgtg attgtcgaat gcgctcaggg agcgatgctg
     1201 gatatcgact acggaaccta cccgttcgtc acctcgtcat ccccaacggc agcgggggcc
     1261 tgccagggtg ccggcgttgc cccgacacaa gttgaacgcg tgatcggggt atacaaggcc
     1321 tatagcaccc gtgtcggtgc aggaccaatg ccgaccgagc tcaacgacca aaccggggag
     1381 cgcatccgga gacggggtaa cgaatacggc accaccaccg gacgcccccg ccgtaccggt
     1441 tggttcgacg gagttgctgc gcgccagact agcaggctga gtggagtcac ggagctggcc
     1501 gtcaccttgc tcgatgtttt ggatatgttc gacgagatcc gattctgttc cggttacagg
     1561 tttgctggcg acgaactcac ccatgtcccc gcttcgctgg aacgtctgga agcagcagca
     1621 gcgacatggg agacgcttcc gggctggaac agggatacca cgagcgcccg ccactacggc
     1681 gacctcccgg agaatgcaca ggcgtatctt gcaaggatcg aagagcaact cggggcacca
     1741 gcgcgctata taggtgtggg gccggctcgc gaacaactaa tcgacaggct ggtgtaacgc
     1801 accatactac gacgatcagc ctgattctcg gaccgaacgc tcgactgtcg ctttcccccg
     1861 gcgggccgtt cgccgacccc ttagaaggat tcgatacggc cgtgtatctc ctccttgggc
     1921 tcgcgggaca tgaggtcccg cactgcttcg gcggctgatt tcccttcaaa gaggacctta
     1981 tacatttgct cggtgatcgg catctccacg ccggtacgat gcgccaactc gaccgccgct
     2041 cgtgtcgtgt ctacaccttc ggccacttca gacatcccgc tcagcacctc ctccagcgac
     2101 gctccggtcg ctaaacctgt gccgacctgg tggttgcgag actgcgggct ggagcaggtt
     2161 gctattaaat ctccaatccc ggagagtccg gcgaaggtca atggctgcgc accatgcgct
     2221 atccccaacc gcgcaatctc cgctattccc ctggtcagga atgccgcctt cgcattgtcc
     2281 cctgcgccga ggccatcacc gatgccggct ccaatcgcga tgatgttctt gagactgcca
     2341 cccgtctcta cccccacgat gtcgatgctg gtgtagatgc ggaacgccgc cccgatcaaa
     2401 gccgactgga tccacgaggc agcctcgacg ttgcgggagg cgacgactgt tgtggccggt
     2461 ttgccagccg ccacttcgga ggagagattt gggccggaaa gggcgcagac cggtgaagtt
     2521 tcgtccgaaa gtacttcttc cacgattgca ctgggccgaa ggagcgttcc gatctcgatc
     2581 cccttcgccg ccgatacgac gatctttccg aggaagagat cgcgaactgg tacgagggct
     2641 ccgcgaagtt tttgcgtcgg gatggccagt atcacggcat ccgccctggc gagaacagcc
     2701 tcgatatcgg tgctcaccga gagcgactct ggaatcgtga cgcctctgag cgaatgggga
     2761 tgcctccgtt cctcggtcag gacccgacat gccgcctcat ccctcacgta caatgctgtt
     2821 ccgttacccg cgcggagaga aaccagcgcc agggttgtgc cccaggcgcc gccgcccagc
     2881 actgccacgc ggggatgctg cgcgtgttgt gtccggttat tcattgacgg cctccagcaa
     2941 tggttcgtag actcgggcca gcgaaagccg cctgctccgg caacgatacg acgagatggg
     3001 accgccgcca accacgctca ggagactccc tgtgacgctc caaatgccgc ttgatactgt
     3061 tgcaatccgc aagattcttc cccaccgcta tcctttcctg ctcgtggacc gaattatcga
     3121 gatgaccccg ggcgaacgcg cggtggggat caagaacgtg acctccaacg aggagttctt
     3181 cgaggggcac ttcccggagt acccggtcat gcccggcgta ctgatcgtcg aagccatggc
     3241 gcaggtcggc ggtgttgccc tgttaagtct ggaagagcat gctggcaaaa tggccctatt
     3301 tgcaggaatt aacaatgtcc gtttcaagcg tcaggtcaaa cccggcgata ccatcctgat
     3361 ggaggtcgcg cttggcacgt tccgcagggg tatcgggacg ggcactgcca cggccaccgt
     3421 ggacggagaa gtcgcttgca ggggtgaact gatgttcgcg ctcgttcccg atcagaccaa
     3481 gggctaatca tcctgatgtc gcctgatccc agcgatgcga tcatcggcat cgacgccagc
     3541 cgaatgcatg tccgggagcg gacgggcacc gagaactact ccgaccagat cattcgggcg
     3601 ctccttgccg cggacgcgga ctggcgatgg cgactgtatg tgaacgggga ggtagcgccg
     3661 ccgtcacttg ccacgccaac gaacgctgaa gtccggccgc ttcaggcccg ccgactctgg
     3721 acgcattacc gcctctcacg tgagatgctg acacgccgcc cgaatctcct ctttgtgccc
     3781 gcgcacgtag ttccgattgt tcacccgcgt acagtcgtga ccatccacga ccttggatac
     3841 cttcatgtgc ccgaagccca cccggtcacc cagaggaaaa tgcttgacct cacaacccga
     3901 tggagcgcga gggtcgcaca tcacatcatt gtgccgtcgg cacagacccg ggaggacctg
     3961 atccaacgct atggcgtcct tgccgggaaa atctcggtcg tgcatcatgg cgtggacccg
     4021 caattccgca aggttgaccg gtcgaacgag caagagctgc gcacccgata caacctgtac
     4081 cgaccatatg tccttgccgt tggcacgatc caaccgcgca aaaatttggc catcctggcg
     4141 cgcgcgatgg aaactggggc tccggaccat gatctggtga tcgcaggcaa gcgaggctgg
     4201 atgtcggacc aggtgttgag cgagctgcgt gagaccagtc tgggcgcccg cttacggatc
     4261 atcgactacg ttcccgatag agatctccca ttgttgtacg cgtcggcttc gattttcgtg
     4321 cagccttccc gattcgaggg attcggtatg cctgttctgg aagccatggc ggcagggaca
     4381 ccggtaatct gcgcctcagg atcgtcgctt accgagattg ccggcgacgc cgcggagttc
     4441 tttcctgcct ccgaccatgc cctgctcgcc caccatctgg ttgagatcct gcaggatcct
     4501 tcccgtatgg ccggcatgcg ggacaggggc gcagcgtggt cagcccgctt cacatgggaa
     4561 cgcgcggcac gggaaacgcg tagaatcttc gaggaagcat tgacctgatg aatccaacgg
     4621 caaatggaaa aaggagcaac gcttgaactc ttcgggacgc accatcggca tcgtcctgtt
     4681 ggctgccggc gttattatcg gtgctgccct cgccgcctgg ctcttcttcg gcgtccagga
     4741 aggcaacctc agggcatcgg gagcggcgtt tggagcagtg ctctttttca gcgtattagt
     4801 gctccccctg gtcggggcag gaatcttttt catcgtcaag gggagtgccg aggccaggaa
     4861 cctggcgcgg gttcaggaac agcgacggct cctggacatg gtgaagactc gtggtcagct
     4921 ccagatcagc gatctcgtcc tcgagcttaa gtcaagcacc gacaaggtta agaacgacat
     4981 ctacgacctg gtgggtcgcg gcctcttctc agggtatgtg gattgggaca aaggtgttct
     5041 ctactcggtc gaggcatcaa agctgcaggg ccgggagact tgcccgaatt gtgggggaca
     5101 ggtagagctc gccggcaagg ggatgattaa atgcccgtat tgcggagctg agatctttct
     5161 ttagccccca ggatgtgcgc cgggcagccg gtaacaggag gacagcccat ggacagactg
     5221 cgaaagttag gaaacgtcga gaaccgcatc accgagctcc tccacaaggt acctgggtat
     5281 aacgggtatc gcgacaagga gaaccggcgc gacgaggaca agcgactgcg agagtccatt
     5341 gccaacgacg tccagaacag cgcgaaccgt ctcacccagt acaacgcgga cctttccgcg
     5401 gcgcgtgagt tcactcacct gtcccgtgtc gaggcactca tcggccagat tcgactattg
     5461 gccgatcggg tccgtactgc ctcctacggc tacggtggca tcttcacgga gcgagaggta
     5521 gacgccgctg ctcttgacca gctccggcaa ttcgacctcg ctctccagcg tgaggtaagc
     5581 agcctcggtg aaaaggtcca agcgctgacg aactctacgc cacccaggga cgatgacgtg
     5641 cgttcccttt caggagagat tgaccgcctg caactgctct ttgatagtcg gacagaggtg
     5701 gttgatcagg cgaggccgac gcgtgacgcc aaagtgttgg aactccttga gaccaacgag
     5761 ccagcggctc cttctcccct gctgtccgtc agcaagggtg acacgctttc ggtgctcggg
     5821 gataacttca tcgccaatgc gacgatctca attgctacgg atcagggccc gctgcagctg
     5881 attcgcgttt ccgaggagga agcgggcgct acgtggctgc tcggctcggg agtcgaaggt
     5941 attgtctccg cccggctcat agagagcctc ggagaaatgc ctccatatca gacgatgcag
     6001 cgcgctgcag ccacgcttga cacagaaaac ggcaggcagg agggcgtcac cgtccagtat
     6061 gctgcgcgaa tgaccggtga aggtgaactc gagcttacgg tcgtctttgg agactctgcc
     6121 cgagtctacc gagggaacga aattcgcgat attgacgtcg aggtttacgg agccgcctga
     6181 ggctcaatta ctctgcttgc aaggagaacc acaccccaat gagcattctg gaccgcatgc
     6241 agcgactcgt tcgcgccaac gtcaacgacc ttatcgaccg ggcagaagac ccggaaaaga
     6301 tgatcgacca gatcctgcgt gacatggaat cgaacatcct cacagcccgt tcgcaggtag
     6361 ccgccatgat cgcccaggag aaggaactgg aggcagatct cgacgagacc aagcaactcg
     6421 ctgccgaatg gggcgacaag gcgaaacgtg ccgtcgatgc aggcaaggac gatctcgcgc
     6481 gcgaggccct ccgacgcaaa cgtgacaatg aagaaaacgc ccgcgtgtac cagcagcagc
     6541 atgaaacaca gttgcagacg gtgagcaacc taaaacacca gctgcaggcg ctggaatcca
     6601 agtaccagca gacgcagagc cagcgggact cgctcatcgc ccgccagcgt cgagcgaaag
     6661 catcccagca ggtggcggag ttcagctcta cccttactcc gttcgacgca agctccgagt
     6721 tggagcgaat ggagcgaaag attcggtcca ccgagtccca ggcggtggcc ctcgcggaga
     6781 tggagtccac gtcgctggat gcccagttcc gtgaactcga ctacgatgtt gatatcgagc
     6841 aggagctgga agcgctcaag ggtggttcga gtgagaccgc gggccagctt gaagcgggcg
     6901 gccagcaatc cggcgcctag atcgaagacg cctgtcgatg gatgcggtca gacacctgac
     6961 cgcatccgtt tcacgtggat gggcacggga agtccgagcc tcgaccatcc cgtccgggag
     7021 atatctgcat ggcaatcctc gaccttatcg agtttcccaa cgaggcgact ggtgacatcg
     7081 tccgccgggt tcccgaagga acatccggcg agttccgtct cggttcccag cttgtcgttc
     7141 gggagagcca acgtgccgtc ttcttccgcg acggcaaggc cttggatgtg ttcgggcccg
     7201 gtcggcacac gctttcgacc aacaacattc cgctactgac cggccttctg ggaatcccct
     7261 tcgggggcga ctccccgttc cgcgccgagg tgtacttcgt ctcgatgcgg gagttcaccg
     7321 acatgaagtg gggttcaagc cagccgatcg tgttccggga caccgagctg ggcatgatcc
     7381 gtctccgtgc cttcggcaca tatagcatgc gggtgagcga cccgcagctg ctcgtctccc
     7441 agattgttgg cgcgcgaggc agctacacca cgtcatcgat tgaagatttc ctccgcagcg
     7501 tcatcctcca ggagttcaca gacctccttg cagatgtcca gacgtccatc ctcgatctcg
     7561 cgagcatggc gaaggaagta gccgacggca ccagagccgc actggcggac gacttctccc
     7621 ggtacggctt tgaacttaca agtttccagg tgcaggcgat cactccaccg gaggaagtcc
     7681 agaagcgtat cgaccagcgc tccggcatgg ccgcgctggg ggacatgcgg acctatacgc
     7741 agttccgtac ggcagaagca attggcgatg ccgccaacaa tcctggttct ggtggcgagg
     7801 gaatctccgc tggtatgggg atgggcgccg gcatgggcat gggggccgca atggcgggag
     7861 ccatgcgcga cgccttccgt gacgatcagc agcagccagt ggatcgaaac cagagacagg
     7921 gcacgggtat ccaaaccacc acatgttccg cctgcaaagc caccatcccg gcaaactcga
     7981 aattctgccc ggagtgcggg acgaatcagc agtcggtgac ttgtccgaag tgccagactg
     8041 agaaccagcc cggcgcgaag ttctgcgtca actgtggaga gaaactcgcg taatcactac
     8101 ctgatacgaa caggaaacgg gcgaggcatg ggagtttccc atgcctcgct tcgtgtgccg
     8161 ggccacgcat cagaataccg tgcgtcagca tcgtacgtac agccgttttc cccggtttta
     8221 cgggcatttc tggtatactt ggacattagg ctctttgcct ttcgtcacac ctcaggagcc
     8281 cgaaaccgtg gatattggaa acgtccggat tgccactatt gaatccgaaa tgcggcagtc
     8341 ctacctcgac tacgccatga gcgtcattgt gcagcgagcg ctgccggatg ctcgtgatgg
     8401 tctcaagccg gtacatcgac gcg
//
LOCUS       DASYWM010000075         6836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_133908, whole genome shotgun sequence.
ACCESSION   DASYWM010000075 DASYWM010000000
VERSION     DASYWM010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133908"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VGR29_03050"
     CDS             <1..1129
                     /locus_tag="VGR29_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV2072597.1"
                     /translation="ISIVNGAFGGATLSGPFATFLARIPVLEPYAGSISGIVVVLTIT
                     YLSLIIGELVPKQLALQRTESVAAMVAPSMKLLATISAPVVWFLSVSSTFVLKLIRAD
                     ESDEPAVTEEEVKLLLGQATEAGIFRRAEQEMVAGVFGLGDRNVGELMTPRHNIVFLD
                     IADPEPVNIERMARAPHAVYPVCDDSTDNVVGIISSRDLWHNQVSGKPTSIREVMRPA
                     LFVPEISPVLTVLQQMRDHKTSTAIVIDEYGGVEGLLTVSDVIEDLVDELGADDGSEE
                     VEGATRRDDGTWLLNGTFPAHEVRELFDIEELEGEQNGRFETIGGFILDQLGHIPRAG
                     ESVEADGHRVEVVDMDGHRIDKVLVTPPNSGSAADVASEDI"
     gene            1231..2313
                     /locus_tag="VGR29_03055"
     CDS             1231..2313
                     /locus_tag="VGR29_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2072598.1"
                     /translation="MSNMPSREPRRPAVSRRAFNMAALSGVTAVAVGRNAAHAAQDST
                     PAPGASPVTTGTLTMYSGRNEALVGGLLDQITEATGVTLDVRYGNTAELAAQILEEGE
                     NTPADLFLSQDAGALGALAKAGRLAELPEELLEEVDPLFRDEEGYWVGLSGRARVLVY
                     QPDLVEESELPESILDLPGSGIGPIGIAPTNASFQTFVTALRLTEGEDAARAWLEELM
                     ASDVVIFDGNGAIVDAVANGEIAVGLVNHYYLYEKRTENPDLTAENYYFTNGDIGGLI
                     NVAGIGVLANSDQQAAALEVVRYLLSEEAQTYFAEQTFEYPLASGVTPSADLPPLDEY
                     EVPDVNLNDLDDLEGTLELLTELGMI"
     gene            2571..4142
                     /locus_tag="VGR29_03060"
     CDS             2571..4142
                     /locus_tag="VGR29_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV2072599.1"
                     /translation="MIWLPAVIIAMFLLLPLVYLIVRAADAGGGAWDLVFRQRTALIW
                     WNTIRLAAGVGATTILIAVPLAWLVTRTDLPFRRFWSTIAALPLVIPSYAGALAVIGA
                     LGPRGFVQGWLEPLGVETLPSIYGYLGAWTMLSLFTYPYVFLAVRAMLRRMDPALEEA
                     SQSLGRGTLRTLVTVTVPRLRPAIAAGALLAMLYTVSDFGVVTLMRYDAFTRVIYTQY
                     RAAFDRTYAAALGILLVVFALGLLVFEARMRGKAAYHRIGSGTGRPPKPIKLGRMRYP
                     AMVFCASIATLSLGIPIGALLYWMITGTSAGRDLQALPASIGNSLLVGLLAALAVTAL
                     SMPVAILLVRYPGPLAWMTERLTYVGYALPGIVIALAFVFMGARFMLPLYQTLPLLVL
                     ALAIRLIPQGVGSIKNSLLQINPRLEDASRCLGCTIYASVRRVSWPLAWPGIAAGAAL
                     VFLTVLKELPITLLLRPTGFDTLATDIWDATGTGQYGRAATSALILIALSAIPTMILS
                     GRSETTAPSEANLNQ"
     gene            4139..5179
                     /locus_tag="VGR29_03065"
     CDS             4139..5179
                     /locus_tag="VGR29_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2072600.1"
                     /translation="MMLKIAGIQKAFGDRAVIDGIDLEVRQGEFVALLGPSGSGKTTL
                     LRLIAGFEDADAGEIRLANRIVVHKDISVPPERRKIGMVFQDYALFPHLSVRDNVAFG
                     LPRGKTRNNRVHEVLERVGLGPWIDQMPQFLSGGQQQRVALARALAPNPSIVMLDEPF
                     SNLDPALRIQVRADVRRILQEAGVTAILVTHDQEEALSIADRIAVLFNGRITQVGTPE
                     DVYERPVSREVAAFVGDAQFLPGTAQETFAETEIGRLELLKPAEGPVDLLIRPEMISL
                     APYADDPKAVPGTVRSRRFFGRDQQVDVVLASGTEMTARVPALHRFAAGERASVSVAQ
                     PVLPFPKQSDPV"
     gene            complement(5578..>6836)
                     /locus_tag="VGR29_03070"
     CDS             complement(5578..>6836)
                     /locus_tag="VGR29_03070"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEV2072601.1"
                     /translation="QMVEATLLEQVSSLRPISLATEASVGDPTITTGEAEIARAQVQE
                     VLAEPYALTEGEQSWTLSPEEVAPALRVVPTSDGLLQLSWDAAVLDALVGHIAAEIDG
                     EAADSWVQDLGTKSWLVPAEEGKSLDRHAFVVSMHEALANGHHEVALTVSVHASPEIT
                     TQEQMAQLGIAEVVGVGDSVYAGSGPGRTNNVEVAAYMVDGTLVAPGEVFSFNAAVGD
                     LFNGMYMDAGSYLDGPNGQSLAGGVCQVSTTVFRAALDAGFPIEEWWQHSYRSPFYEM
                     GGWSPGFDAAIVQDSAVPEESTDLQFRNTTDSWLMVSAKTVDGELKVELLGANTGYAV
                     TFNEPVVEVVEWATDEVSVVTDEQLPPGTVEEQAAMDGLRVTVVRYVHDAEGNLVSED
                     AFVSTYGSYGAIRRVSPDMAEVASGQ"
BASE COUNT         1217 a         2236 c         2058 g         1325 t
ORIGIN      
        1 gatcagtatc gtcaatggtg cgttcggtgg tgccaccctc tcgggaccgt tcgccacctt
       61 cctggcgagg atccctgtgc tcgagcccta cgccgggagc atcagcggca tcgtggtggt
      121 gctgaccatc acctacctgt cgttgatcat cggcgagctc gtccccaaac agctggcact
      181 ccagcgcacg gagagcgtcg ccgcaatggt tgcaccgtcg atgaagctcc ttgcaaccat
      241 ctccgcgcca gtcgtctggt tcctctcggt ctccagcacg tttgtcctca agctcatccg
      301 cgccgatgag tcagacgaac cagccgtcac cgaggaagaa gtcaagctcc tgcttgggca
      361 ggcgaccgag gccggtatct tccggcgcgc cgaacaggaa atggttgccg gagtgtttgg
      421 gctcggcgac cgcaacgtcg gtgagctgat gacaccgcgg cacaatatcg tctttctcga
      481 catcgcggac cccgaacccg taaacatcga gcggatggca agagcacccc acgctgttta
      541 cccggtctgc gacgattcca cggacaacgt ggtgggcatc atcagctcgc gcgatctctg
      601 gcataaccag gtttcaggca agccgacatc catccgggaa gtgatgcggc ccgcgctctt
      661 tgtccccgag atctcccccg tgctcaccgt gctccagcag atgcgagacc acaagacctc
      721 aaccgcgatc gtgatcgacg agtacggcgg cgttgaaggt ctcctcacgg tcagcgacgt
      781 catcgaagat ctggtcgatg agctcggtgc cgacgacggc agcgaagagg tcgaaggggc
      841 aacccgccgg gatgacggca cctggctgct caacggtaca tttccggcgc atgaagtgcg
      901 ggagcttttc gatatcgagg agcttgaagg cgagcaaaac gggcgcttcg agacgatcgg
      961 cggcttcatc ctcgaccagc ttggtcatat cccgcgggcg ggcgagagcg tggaagccga
     1021 cggtcaccgt gtcgaggtcg tcgatatgga cgggcatcgg atcgacaagg ttctcgtcac
     1081 tcctccgaac tcgggaagtg ccgccgatgt ggcatcagag gacatttagg accagccgat
     1141 gttgacaccg aaacgcggca acgctaggct ttgtccacga tatccgaccg tcggggtcag
     1201 gattggtgag tgtccgagga gtgttcacga atgtcgaata tgccatcccg cgagcccaga
     1261 cgtccagctg tgagccgtcg cgccttcaac atggcggcgt tgagcggtgt cacggccgtg
     1321 gcagtcggcc gcaatgctgc gcacgctgcc caggactcca cgccggcgcc gggtgcatca
     1381 ccggtcacca ccggcacgct gacgatgtac agcggccgga atgaggcgct tgtcggggga
     1441 ctgctcgacc agatcaccga ggcgaccggc gtcacgctgg acgtgcgcta tggcaatacc
     1501 gccgagcttg ccgcccagat cctcgaggag ggcgagaata cgccggccga tctcttcctc
     1561 tcccaggacg ccggagccct gggtgctctc gccaaggctg gccgactggc cgaattgccc
     1621 gaggagctgc ttgaggaggt tgatcccctc ttccgcgacg aggagggcta ctgggtcggg
     1681 ctttcgggca gggcccgcgt gctcgtgtac cagccggacc tcgtcgaaga gagcgagctg
     1741 cctgaatcga ttctcgatct gccgggatcc ggtattggac cgatcggtat cgctccgacc
     1801 aacgcctcgt tccagacctt tgtgacggcc ctgcgcctga ccgagggtga ggatgctgcc
     1861 cgcgcctggc tcgaggagtt gatggcctcc gatgtcgtca tctttgacgg caacggcgcg
     1921 atcgtggacg cggtcgccaa tggggagatc gccgttgggc tcgtgaacca ctactatctc
     1981 tatgaaaagc ggaccgagaa cccggatctc accgccgaga actattattt caccaacggc
     2041 gatattggcg ggctcattaa cgtcgcgggc attggtgtgc tggccaacag cgaccagcag
     2101 gccgcagccc tggaggtggt tcgctacctc ctgagcgagg aagcacagac ctacttcgca
     2161 gagcagacgt tcgagtatcc gcttgcctcc ggtgtgacgc cgagcgcgga cctgccgccg
     2221 ctggatgagt atgaggttcc agatgtgaac ctcaacgacc ttgatgatct cgagggtacg
     2281 cttgagctgc tgaccgagct cggcatgatt taggggcggc cagcgagggt tgcctttgat
     2341 cgcacgtacg agcgtgtgcc accacaaacg gcacatgttg cgggccgcgg aggcagtgtc
     2401 atgtatgtgg caccgcctcc gcggagaaca ggggcgttgt gttaagggag acggcatcgt
     2461 ttccagaaca acaagtgaag tcgcccgaac ggttatcccc cgggcggctt cgccgtgcac
     2521 catctgccat cccctggcgg cagcgctatc gtgcccggag tgccccggtc gtgatctggc
     2581 tccccgccgt gatcattgcg atgtttcttt tgcttcctct tgtctatctc atcgtgcgcg
     2641 cggccgacgc aggaggaggt gcctgggatc tcgtcttccg ccagcgcacc gccctcatct
     2701 ggtggaacac catacgcctc gctgccggtg tcggggcaac gacgatcctg atcgccgttc
     2761 cactcgcctg gctggtgacc aggacagatt tgcccttccg tcgcttctgg tcgaccatcg
     2821 cggcactgcc gctggtcatc ccctcctacg cgggggcact ggcggtgatc ggcgcgctgg
     2881 gtccgcgagg ttttgtacag ggatggctgg agccccttgg cgtcgagacg ttgcccagca
     2941 tctacggcta tctcggcgca tggacgatgc tctcgctttt tacctacccc tatgtcttcc
     3001 tcgcggtgcg ggccatgctg cgacgcatgg acccggcgct tgaggaagca agccagagcc
     3061 tcgggcgcgg aaccctgcgg accctcgtga ccgtcaccgt cccgaggctt cgccccgcga
     3121 tcgccgcagg tgcgctgctc gcgatgctct ataccgtcag cgatttcggt gtcgtgacgc
     3181 tcatgcgcta cgacgcattc acacgcgtca tctatactca ataccgggcc gcattcgatc
     3241 gcacctatgc agcggcactg ggtatcctgc tggtcgtctt cgccctgggg ctgctggtct
     3301 tcgaggcaag gatgcggggc aaggccgcgt atcaccggat cggtagcggc accggtcgac
     3361 cgccgaagcc gatcaagctg ggccggatgc gttaccccgc catggtcttc tgtgcctcca
     3421 ttgccacgct cagcctcggc attccgatcg gcgcgcttct gtattggatg atcaccggca
     3481 cgtcagcggg tcgtgatctc caggcgctcc ccgcttcgat cggcaactcc ctgctcgtcg
     3541 gcctgctcgc cgcgctcgcg gtcacggcgc tctcgatgcc ggtcgcgatc ttgctcgtcc
     3601 gctaccccgg ccctctcgcc tggatgaccg aacgcctgac ctatgtaggc tatgcccttc
     3661 ccgggatcgt cattgccctc gcctttgtgt tcatgggcgc gcggttcatg ctccctctct
     3721 atcagaccct gccgctcctc gtgctcgccc tcgccatccg gttgatcccc cagggcgtcg
     3781 gctcgatcaa gaactcgctg ctccagatta acccgcgcct ggaagacgcc tcccgctgtc
     3841 tcggctgcac catttatgca tcggtgcggc gcgtctcctg gccgcttgcc tggccgggaa
     3901 ttgcggcagg cgccgcactc gtcttcctga cggtcctcaa ggaactcccg atcacgctcc
     3961 tcctccgccc aaccgggttc gatacccttg caacggatat ctgggacgcc acgggcaccg
     4021 gtcagtatgg acgtgcggcg acttcagcgc tcatccttat cgcgctttca gccatcccga
     4081 caatgattct ttcagggcga agcgagacaa ctgccccgtc cgaggccaat ctcaatcaat
     4141 gatgctgaag atcgcgggca tccagaaagc attcggggat cgggccgtga tcgacggcat
     4201 cgacctggag gtgcgccagg gcgaattcgt cgcgctgctt ggtcccagtg ggagcggcaa
     4261 gacgaccctg ctgcgcctga tcgcaggttt tgaggatgcg gatgcgggcg agatccgtct
     4321 agctaaccgg atcgtcgtcc acaaggacat tagtgtgccg cccgagcggc gcaagattgg
     4381 gatggttttc caggactacg cgctctttcc gcatctctcg gtacgggaca acgttgcctt
     4441 cggcctgccg cgtggaaaga ctcgcaacaa ccgtgtacac gaggtgctgg agcgggtcgg
     4501 gcttggcccc tggatcgacc agatgccgca gttcctctcc ggtgggcaac aacaacgcgt
     4561 cgcgctggca cgagcgctgg cccccaaccc atcgattgtg atgctcgacg agcctttctc
     4621 caaccttgat ccggcactgc gtatccaggt ccgggcggat gttcgccgca tcttgcagga
     4681 ggccggtgtc acggcgatcc tcgtgaccca cgaccaggag gaggccctta gcatcgccga
     4741 ccgcatcgcg gtcctgttca acggtcggat cacgcaggtc ggtacaccag aggatgtcta
     4801 cgaacgtccc gtcagccggg aggttgctgc attcgtcggt gatgcccagt tcctgccggg
     4861 cacggctcaa gagacgttcg ctgagacgga gattggaagg ctggaattac tcaagcccgc
     4921 cgaggggcct gtcgatctcc tgatccgacc cgagatgatc tcccttgcgc cctacgccga
     4981 tgaccccaaa gccgttccag gcaccgtacg atctcgcagg ttttttggcc gggaccagca
     5041 agttgacgtt gtgctggcaa gtgggacgga gatgaccgcg cgcgtccctg cgctccaccg
     5101 gttcgccgct ggggagcgcg cgtcggtgtc cgtcgcgcag ccagtcctcc ccttcccaaa
     5161 gcaaagcgat ccagtctaga acgagagaca ttaccccgtt ccagggatac tcctgacacc
     5221 gggcggtgcc tcactcctgg cggccctcct ggataactct gctccggtcg ttggaggata
     5281 gagcggttct cgatgcacgt acccgccctg atatcggcct tcggtggatc gggctatggg
     5341 gccgctcccg ctcaaaccct tcgggtttgc ctatgacatc gcttcgcggg tgtggaccct
     5401 acagtggcca cttcgttcga tcaccgcttt agccaatgag gtcccgtaca cccacatcgt
     5461 gccggatggg ccaaccaatg tgccccacct ttcagcacca cagttcggga acatcgaagc
     5521 ggggcaccga aaggaaccgg tgccccgctg gatagcatgc cctgattgaa ccagctccta
     5581 ctgtccagaa gcgacctcgg ccatatcggg gctcactcgc cggattgcac cataggaacc
     5641 ataggtggag acaaacgcgt cctcagagac gaggttgccc tccgcgtcat ggacgtagcg
     5701 gaccacggtc acccgcagcc catccatcgc ggcctgttct tcaacggtac ctggtgggag
     5761 ctgctcgtcg gtgaccaccg acacctcatc ggtcgcccac tcaaccacct caaccacggg
     5821 ctcattgaag gtaacggcgt agccggtgtt tgcccccaga agctccacct tcagctcgcc
     5881 atccaccgtc tttgcgctca ccatcagcca ggaatcggtc gtattccgga actgcaggtc
     5941 tgtgctttcc tcgggaacgg cgctgtcctg gacgatcgcg gcatcaaaac cgggtgacca
     6001 accgcccatc tcatagaagg gggagcggta ggaatgttgc caccactcct cgatcgggaa
     6061 ccccgcatcc agcgcggcac ggaagaccgt ggtcgacacc tggcagaccc ccccagcgag
     6121 cgattgcccg ttcggtccat cgagatagga gccggcatcc atatacatgc cgttgaagag
     6181 gtcaccgacc gctgcattaa aggagaagac ctcaccgggc gcgaccagtg tgccatcgac
     6241 catataggcc gcgacctcga cattgttcgt ccgaccggga ccagaaccgg catagaccga
     6301 gtcacctacg cccaccacct cggcgatgcc gagctgagcc atctgctctt gggtcgtgat
     6361 ctcgggtgag gcgtggacac tgacggtcag agcgacctcg tggtgaccgt ttgccaacgc
     6421 ctcgtgcatc gatacgacaa aggcatgccg gtcaagtgat ttcccctctt ccgccgggac
     6481 caaccacgac ttcgtcccca gatcctgcac ccaggagtca gctgcttcac catcgatctc
     6541 cgccgctata tgcccgacaa gcgcatcgag cacggcagca tcccaggaaa gctgaaggag
     6601 accatcggaa gtcggcacga cacgcaaggc cggagcgacc tcttccggtg agagcgtcca
     6661 tgattgctca ccctcggtca gggcatatgg ctccgccagc acctcctgga cctgtgcgcg
     6721 ggcgatctct gcttccccgg tcgtgatcgt cgggtctcct accgacgctt ctgtcgcaag
     6781 ggagattggc cgcagcgagg aaacctgctc caggagcgtt gcctcgacca tctgtc
//
LOCUS       DASYWM010000076         8757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_434326, whole genome shotgun sequence.
ACCESSION   DASYWM010000076 DASYWM010000000
VERSION     DASYWM010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434326"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VGR29_03075"
     CDS             complement(<1..622)
                     /locus_tag="VGR29_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV2072602.1"
                     /translation="MAWRSLRAASTRLVRKRVDLQVENVENLPPNGPVIIAARHFHHL
                     YDGAVMMSIVPRPLRILVGLDWVRNPVGKVMMERLCRAASWPVVMGRGGTLPRHDREK
                     SRALRQAISDSLKTLRDGDALMVFPEGYPNIDPGYTPKEDESVFLPFQPGFVRLATIA
                     ASQGLRVPIVPVGLAYIRSEPWQVIVRFGEPISLTHRHEERSVLQQV"
     gene            complement(624..1496)
                     /locus_tag="VGR29_03080"
     CDS             complement(624..1496)
                     /locus_tag="VGR29_03080"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HEV2072603.1"
                     /translation="MKSPNGTWIPLVLFFAHIGALVFGLVGLLIMLPNPELWSSDPRA
                     VRVFDWSMEYAGATHIIFGALAMFAFGVVTIGWRKTSIFAAVSYALSLASELMGTGTG
                     WPFGNYAYTDFLGYKVLDHVPYTIPLSWFYMGFASYLLGAVLADRLVSRQRTLWSLML
                     GTWFLTVWDLVLDPAMAHESLRVQFWVWDETGPYFGMPVKNFIGWSVTGLLFMAISRL
                     LWREEVRPQSVPMLFPLAVYTANVIFAMVLSAGVDLWMPILLAAVLGLIPAGVAAYRS
                     VDVMPQHPRLARDA"
     gene            complement(1493..2638)
                     /locus_tag="VGR29_03085"
     CDS             complement(1493..2638)
                     /locus_tag="VGR29_03085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2072604.1"
                     /translation="MKRIITLFTLVQSVLAIRVFYRMAVTADSKRVRRDPHLRDLGRV
                     SVIVPVLNEVDRLSPCLDGLTSQGVEVAEILVVDGGSTDGTPDLVRHWETLDDRVCLI
                     QAGPVFHGSNGKAHGMQVGLEHSKPENGWVLTIDADVRPAPLLVCSLLAHASDEQVPA
                     LSAATLQSLSGAAEGLVHPSMLTTLVYRFGIPGHATQRVDQVQANGQCFLVQREVLEA
                     VGAFASVADSICEDVTLARTIAARGYPVGFYEVDGLVSVQMYNGWQDAWDNWTRSLPM
                     RDGFTRWWSALGLAEVMAVQALPLWLVPLYRWRAGDPRFATVLNIALLLSRVGVLVGV
                     SRAYSRRPWTYWLSSLCDLPVAFRLWYMWGRRRHFWRGREIISGHSS"
     gene            2740..4272
                     /locus_tag="VGR29_03090"
     CDS             2740..4272
                     /locus_tag="VGR29_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEV2072605.1"
                     /translation="MSQRIVVIGSGFGGLAAAVRLAKRGHDVTLIDKRDKPGGRAYVY
                     EQDGFSFDGGPTVITAPWMITELFELAGKRAGDYVTLVPVDPFYRIFFHDGTHFDYTG
                     DAGEMLAGIRDFSEYPGDVEGYRRFTALTERVFAKGFTELASKPFLSPVDMLKVAPDL
                     MRLQSYRTVYGLISRFVKDERLRQVLSFHPLLVGGNPFQTTSIYTLIHHLEREWGIWF
                     AMGGTGAIVDALVRLFGELGGTLMLNAEADEILTGGRRATGVRLTNGTELPADIVVCN
                     GDVAAAYQRLLPTGLRRKYTDKRLARMRYAMSLFVIYFGTDRRYREGGDTRLSHHNII
                     LGPRYKELLNDIFNKKTLAEDFSLYLHMPTLTDPSLAPPGCETFYVLSPVPHLGGTTD
                     WTREMKPYRDKIMSFLEVHYLPDLSRHIVSEHIIDPRHFRDDLNSYQGSAFSVEPILT
                     QSAYFRPHNRSEELPNLYFVGAGTHPGAGLPGVLSSAVIADNLILESMDSGPSRKTSV
                     PA"
     gene            complement(4279..5259)
                     /locus_tag="VGR29_03095"
     CDS             complement(4279..5259)
                     /locus_tag="VGR29_03095"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEV2072606.1"
                     /translation="MQSVNDGVYRSPGLSDWMHCEEIARHHGRSFFLASRLLPPDRQR
                     GVISTYAYCRVADDIVDRASGEGPTATAEALARWTSQLTAPDEPIAVAFAHTRERYAI
                     PMQPVSDLLSGIQMDLTVTRYENWEDLHRYCYHVAGTVGLMVAPILGCRDSKALRHAA
                     DLGIAMQLTNILRDVAEDAQMGRIYLPLDEISAFGCDPDAILAGQPGRHFRELMAFQI
                     DRARALYASALMGVYALSPSGRLTTLAATKLYSGILSEIEALDYDVYRMRAHVPTARK
                     LRSMAGASTDFLRMSVMFRDQTAAVGVLPDMEALYVSGRGQQAGADARRT"
     gene            complement(5342..6439)
                     /gene="fni"
                     /locus_tag="VGR29_03100"
     CDS             complement(5342..6439)
                     /gene="fni"
                     /locus_tag="VGR29_03100"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV2072607.1"
                     /translation="MSIPTGTERRKTDHIRINLEEQVEPHDATTGLERYRLVHNALPE
                     LNLDTIDLSTTILGHSLAAPLLISSMTGGVEEGWQITRRLAQAAQQLGCAIGVGSQRA
                     AIEDPARSRFFQVREVAPDILLFANLGAVQLNYGFGPEECRRAVDMIGADALILHLNP
                     IQEALQSDGNRDFSGLMGKIGEVCRALEVPVVVKEIGFGISADVARRLQEQGVAAIDA
                     SGAGGTSWSAVEHYRSAGPMSRRISSTFIDWGIPTATSLQMARRGAPGVPLIASGGLR
                     TGVDAAKAIALGAALAGFAGPMLKAAAVSTEDTYDMLAVLIEELRLAMFGCGAGTIAQ
                     LRQAPLLKDGVEMAVLDGPVNPDRATSVRKP"
     gene            complement(6518..7957)
                     /locus_tag="VGR29_03105"
     CDS             complement(6518..7957)
                     /locus_tag="VGR29_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEV2072608.1"
                     /translation="MSTITRVYRSRVPAAPTDVLAWHDNPCVFERLTPPWKKVRVVEA
                     QGSIAPGDSKHLRVSIAGPLEFSWKLVHGEIPNQAGFADIQQEGPFRSWRHEHRFFPD
                     GEADTMLEDRLTYDLPFGIAGRFLGGKRVESELDRLFRFRHRRTQIDLSRHSHFAATQ
                     PLRIAVTGSTGLVGHRLVAFLRSGGHEVLRLVRHPVQANDEVFWDPESSRIDAASLEG
                     LDAVIHLAGVSIAGGRWTRSRKAAILSSRLDGTHLLAKTLAHLSQPPRVFVPASAVGY
                     YGNAGREVLTEHSSPGTGFLAEVCKGWEAASQPASAAGIRVVHPRFGVVLAAEDGLLP
                     LISRVFQLGGGGPLGSGEQYMSWIALDDLIGILLHAVTDDSLKGPVNAVAPEPVTNRE
                     FSSILARVLVRPAFVRTPAAAMRLAGGELADELILASQRALPAKLSDQGFQFAFPTLE
                     DALRHELGRYDGAGSWDPAVSKANDRRVA"
     gene            complement(8321..8554)
                     /locus_tag="VGR29_03110"
     CDS             complement(8321..8554)
                     /locus_tag="VGR29_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072609.1"
                     /translation="MVVPSPVHFGYEQLVAVKEGCQLSAPSTGFRMIGVSSLILGEHG
                     PNSVKFPVLAKEMSTTEIGGWNVPDSQPAISLI"
BASE COUNT         1855 a         2743 c         2489 g         1670 t
ORIGIN      
        1 ctacctgttg aagtacggat cgctcctcat gcctgtgtgt gagcgaaata ggctcgccga
       61 agcggacaat gacctgccaa ggctcactac ggatatatgc aagacccacc ggcacgatgg
      121 ggactctcaa gccttggctg gcggcaatag tagcaaggcg gacgaaccct ggctggaacg
      181 gcagaaacac agattcgtct tccttcggcg tgtagccggg gtcgatgttc ggatagccct
      241 ccggaaagac catcagcgca tccccatcac gcaacgtctt cagcgaatcg ctgatcgcct
      301 ggcgcaaggc gcgggacttt tcgcggtcgt gcctcggcag tgtgccaccc cggcccatca
      361 ccaccggcca tgaggcagcc ctgcagaggc gctccatcat caccttgccg accgggtttc
      421 gcacccagtc aagcccgaca aggatgcgca gtggtcgcgg cacaatcgac atcatcactg
      481 caccatcgta gagatggtgg aagtgccttg cagcgattat gaccggaccg ttcggcggca
      541 aattctcgac gttctcgacc tggagatcga cccgctttcg caccagcctc gtgctcgcgg
      601 cccgcaggga ccgccacgcc atatcaggca tcccgcgcca gtcgtgggtg ttgcggcatg
      661 acgtccacac ttctgtaggc cgccacaccg gcgggaatca gtcccaggac agcagcgagc
      721 agaatcggca tccagaggtc gacacctgcg ctgaggacca ttgcgaatat gacgttggcg
      781 gtatacacgg cgagagggaa gagcatggga accgattgtg gccggacttc ctctcgccag
      841 agaaggcggc tgatcgccat gaagaggagg ccggtcaccg accacccaat gaagtttttg
      901 accggcatgc cgaaataggg cccggtctcg tcccacaccc agaactgcac ccgaagtgac
      961 tcatgtgcca tcgcagggtc aagtaccaag tcccacacgg tgaggaacca ggttcccagc
     1021 atcagcgacc ataatgtgcg ctggcgcgag accaggcgat ccgcgagtac cgcgcccagg
     1081 agataggagg caaaccccat gtaaaaccag gatagcggga ttgtgtaggg cacatggtcc
     1141 agcaccttgt acccaaggaa atcggtatag gcatagttgc cgaagggcca ccccgtcccc
     1201 gtccccatca gttctgacgc aagcgagagc gcgtaggaga cggcggcaaa gatactcgtc
     1261 ttccgccatc caatcgttac caccccgaac gcgaacatcg ccagggcacc aaagatgatg
     1321 tgggtcgcgc cagcgtactc cattgaccag tcgaagaccc gtacagcgcg gggatcactc
     1381 gaccagagtt cgggattggg cagcatgatc agtaagccga ctagtccaaa gaccagcgcc
     1441 ccgatgtggg caaagaacag gacaagggga atccatgtac cgttgggaga tttcatgatg
     1501 aatgtcctga gataatctcg cgtccgcgcc aaaagtgccg ccgccggccc cacatgtacc
     1561 agagcctgaa ggctaccggc aaatcacaca aagaggagag ccagtaggtc cagggacgcc
     1621 ggctatatgc ccgcgacacc cctacaagaa cccctacccg cgaaaggagc agggcgatat
     1681 tcaataccgt cgcgaagcgc ggatcaccgg ctcgccaccg gtacagcggg acaagccaca
     1741 ggggcaatgc ttgtaccgcc ataacttccg ctagcccgag tgcgctccac catcgcgtga
     1801 agccatcccg catcggcagc gaacgcgtcc agttgtccca ggcgtcttgc cagccattgt
     1861 acatctgcac cgacaccaga ccatcaactt cgtaaaagcc aactgggtat cccctggccg
     1921 caattgtcct cgcaagcgtc acatcctcgc agatcgagtc agcaaccgag gcaaaggctc
     1981 ccaccgcctc gagcacttct cgctgcacca ggaagcactg cccattggcc tggacctgat
     2041 ccacccgctg cgtggcgtgt ccagggattc cgaagcggta cacaagagtg gtcaacattg
     2101 acgggtgcac caggccctcc gccgctccgg aaagcgattg gagcgttgcg gcactcagcg
     2161 cgggcacctg ctcgtcggag gcgtgggcca gaagggagca cacgagcaga ggcgccggac
     2221 ggacatcggc atcgatcgtc aggacccagc cgttctccgg cttgctgtgc tcaaggccaa
     2281 cctgcatccc atgcgccttg ccattggagc cgtggaacac cggtccagct tggatcagac
     2341 agacacggtc gtccagcgtt tcccagtgac gcaccaggtc cggcgtgcca tccgtcgacc
     2401 caccatcgac tacgaggatc tctgccacct cgactccctg tgaggtcagg ccatcaagac
     2461 agggcgacag ccgatctacc tcgttcaaca cgggaacgat cacgctcacc cgtcccaggt
     2521 cccgcaggtg gggatcacgc cgcacgcgct tgctgtccgc cgtgactgcc atgcggtaga
     2581 agacccggat tgccaggacc gactgcacaa gggtgaagag tgtgataatc cttttcatca
     2641 ccgtgctcgc cccacccgcg tgatggaatg atcgatagcg ggcacgagtc ttggcacagc
     2701 atgaacaccc ggtattgcgg tgaatggaac agggtgcaga tgagccagcg aatcgttgtc
     2761 atcgggagcg gatttggcgg cttggctgct gcggtgcgtc tggctaagcg tggccacgac
     2821 gtcacgctga tcgacaagcg ggacaagccg ggcgggaggg catacgtcta cgagcaggac
     2881 ggattctctt ttgatggagg acccactgtc ataaccgcgc cctggatgat caccgagctc
     2941 ttcgagcttg cgggtaagag agccggggac tatgtcactc tggtgcccgt cgatccgttc
     3001 tatcgcatct ttttccacga cggcacccac ttcgactaca ccggcgacgc gggcgagatg
     3061 cttgccggga tccgggactt cagcgagtac cccggggatg ttgaaggcta ccgccggttt
     3121 acggcgctga ccgaacgcgt cttcgccaag gggttcaccg aacttgcgtc gaagcccttc
     3181 ctgagccccg tggacatgct caaggttgct ccggacctga tgcgcctgca gagctaccgt
     3241 acggtgtatg gactgatctc gcggttcgtg aaggatgaac gtctgcgaca ggtcctctcg
     3301 tttcacccgc ttctggtcgg cggcaatcca ttccagacca caagcatcta cacgctcatt
     3361 caccaccttg agcgcgaatg gggcatctgg ttcgccatgg gagggacggg tgcaatcgtt
     3421 gatgcgcttg tacggctgtt cggggagctt ggtggaacac tgatgctcaa tgccgaggcc
     3481 gacgagatcc tgacaggggg ccgccgcgca accggcgtga gattgaccaa tggcaccgag
     3541 ttgccagcgg acatcgtggt gtgtaacggg gacgtcgcgg ctgcttacca acgtcttctc
     3601 ccgaccggcc tcaggcggaa atacaccgac aagcgccttg ctcggatgcg ttacgccatg
     3661 tcgctcttcg tgatctactt cgggaccgat cgccgctacc gcgagggcgg cgataccaga
     3721 ctgtcccacc acaacattat ccttgggcct cgatataagg agctgttgaa cgatatcttc
     3781 aacaagaaga ctctcgcaga ggatttctcc ctgtacctcc atatgccgac attgaccgat
     3841 ccctcgctgg ctcctcccgg ctgcgaaacg ttctacgtcc tgtctcccgt accgcacctt
     3901 ggcggcacaa ccgactggac cagggagatg aagccttacc gcgacaagat catgtcgttc
     3961 ctggaggtgc attacttacc cgatctctct cgccatatcg tatcggaaca catcattgat
     4021 ccccgccatt ttcgtgatga cctgaacagc tatcagggct ccgccttctc ggtcgaacca
     4081 atcctcaccc agtcggccta tttccgacca cacaatcggt ctgaggagtt gcccaacctc
     4141 tactttgtgg gagcggggac gcaccctggg gctggcctac cgggagtact cagttcggcc
     4201 gtcatcgctg ataacctgat cttggagagc atggatagtg gtccgtccag gaaaacctct
     4261 gtccccgcat gactgtcctc atgtccggcg tgcgtccgcg ccagcctgct ggcccctgcc
     4321 cgagacatat agagcttcca tatctggcaa cacgccgacc gctgctgtct gatcacggaa
     4381 catcaccgac atgcgcaaga agtcggttga cgctcccgcc atggaacgca gctttcgtgc
     4441 tgtagggaca tgggctcgca tccggtagac gtcgtaatcg agcgcctcga tctcactcag
     4501 gatgcccgaa tagagcttgg ttgctgcgag cgtagtcaaa cgcccgcttg gcgataatgc
     4561 atatacgccc atgagggcac tcgcatagag cgcgcgcgcc cggtcgatct ggaaggccat
     4621 cagttccctg aagtggcgac caggctgtcc tgccaggatg gcatccggat cacatccgaa
     4681 ggccgaaatc tcgtccaggg gcaggtagat ccgccccatt tgcgcatctt cggcaacatc
     4741 gcgaaggatg ttggtgagct gcatggcgat gcctaaatcg gcggcatgcc gcagcgcttt
     4801 ggaatcacga cacccgagga tcggcgccac catcagccct acggtgcctg ccacgtgata
     4861 gcaatatctg tgaaggtctt cccagttctc atagcgagtg acagtcaaat ccatctggat
     4921 tcccgaaagg agatcgctga ccggctgcat cggtatggca taacgttcgc gagtgtgagc
     4981 gaacgccacc gcgatgggct cgtccggtgc tgtcaattga ctggtccagc gggccagtgc
     5041 ttctgcggta gcggttggtc cttctccact tgcccggtcc acgatatcgt ccgcaacacg
     5101 acagtacgcg tacgtggaaa tgacgccccg ctgccggtca gggggcaaca gcctggacgc
     5161 aagaaaaaaa gatcgaccat gatgccgggc gatctcctcg cagtgcatcc agtcagacag
     5221 tcctggggaa cggtagaccc cgtcattaac ggactgcatg attaccctcc tcctgtttcc
     5281 cggtgttccc gctccggcag ccaactacca ttcttacccc acaagccccg tctgccgaga
     5341 gttaaggttt tcgaaccgat gtcgctcggt ccggattcac cggtccatcc aggacagcca
     5401 tctctacccc gtctttcaac aggggcgcct gccgcagttg agcgatagtg ccggcaccac
     5461 agccaaacat ggctaatctc agctcctcta tcaggacggc aagcatgtcg taagtatctt
     5521 ccgtactgac cgccgccgcc ttgagcatcg gcccggcgaa accggcgagg gctgcaccta
     5581 acgcaatcgc cttggctgcg tcaacaccgg tacgcaatcc accgctggca atcaacggca
     5641 ctcctggtgc acctcgcctt gccatctgca ggctcgtggc ggtcgggata ccccagtcga
     5701 taaatgtgct actgattcgc ctcgacatgg ggcctgctga ccggtaatgc tcgacagcgc
     5761 tccatgatgt acctccggcg ccgcttgcat cgatggctgc gacgccctgc tcttgcaggc
     5821 gtcgggcgac atccgcggag ataccaaacc cgatctcttt gacaacgacc ggcacttcaa
     5881 gcgccctgca aacctcaccg atcttgccca tcaagccgga gaaatcacgg ttgccgtcac
     5941 tctggagcgc ttcctggata ggattaagat gtaggatcaa ggcgtccgcc ccgatcatat
     6001 ctactgctcg gcgacattcc tccggtccga acccgtagtt cagctgcacc gcaccaagat
     6061 tggcgaacag gagtatatcg ggagcaactt cccgcacctg aaagaagcgc gacctcgctg
     6121 gatcctcgat tgccgcccgc tgggatccca cgccgatggc gcagccgagc tgctgtgccg
     6181 cctgcgccag gcgacgggtg atctgccagc cctcttccac tccacctgtc atagacgaaa
     6241 tgagcagcgg cgctgcgagg gaatgcccga gaatggtggt agaaaggtca atagtgtcga
     6301 ggttcagttc aggcaacgca ttgtgaacca gtcggtaccg ttccagccca gtggtagcgt
     6361 catggggctc gacttgttcc tccagattga tccggatgtg gtcggtctta cgtcgctccg
     6421 ttccggtggg aatcgacata cgcgcgttgc tcctgtccgg gttgccgcca ctcaaaccag
     6481 gatatatgaa gggccggtcg aatggtctgc tcgtagacta tgcaactctt cggtcgtttg
     6541 ccttactcac tgctggatcc caggaacctg caccgtcata ccggccaagc tcgtgtctca
     6601 gtgcgtcctc caacgtcgga aacgcgaact gaaacccttg atcgctcaac tttgctggta
     6661 gcgcccgttg gctggcaagt atgagctcat ccgcaagttc tccgccagca agccgcattg
     6721 ccgccgcggg tgtccgcacg aatgccggcc ggaccaacac gcgggcgaga atggacgaaa
     6781 actcgcggtt ggtgactggc tctggtgcaa cggcgttcac cggacccttc agcgagtcgt
     6841 ctgtaactgc atgcaggaga ataccgatca gatcatcaag cgcaatccag ctcatgtatt
     6901 gttccccgct ccccagcggc cctcccccgc caagctggaa gacgcgtgaa atcagtggca
     6961 acaatccgtc ttccgcggcg agcaccaccc cgaatcgggg atggacaacg cggatacccg
     7021 ctgccgaggc tggctgtgac gccgcttccc agcccttgca cacctcagcc aggaatccgg
     7081 ttcccgggga tgaatgctcg gtcaatactt ctcggccggc attcccgtaa tatccgaccg
     7141 ctgaggcggg cacgaatacg cgcggtggct gcgagaggtg agccagcgtt ttggccaaca
     7201 aatgggtccc gtccaaccgg ctggagagga ttgccgcctt tcgcgacctt gtccagcgcc
     7261 caccggcaat cgaaacacct gcaaggtgga tcactgcgtc cagcccttca agcgatgccg
     7321 catcgatccg cgagctttcc ggatcccaga acacctcgtc attcgcctga actggatggc
     7381 gcacgagtct caacacttcg tgtccaccgg atcgcaggaa cgcgacaagc cggtgaccaa
     7441 caagacctgt ggagccagtg actgcgatcc gaaggggttg tgtcgccgca aagtgagagt
     7501 gccgactgag gtcgatctgt gtgcggcggt gacggaaccg aaagagtctg tccaactctg
     7561 actcaacgcg tttgccgcca aggaagcgcc cggcgattcc aaaggggagg tcgtacgtca
     7621 gacggtcttc cagcattgtg tccgcctctc catcggggaa gaaccggtgc tcgtgcctcc
     7681 aggaacgaaa gggaccctcc tgctggatgt ccgcgaagcc tgcctgattc gggatttcac
     7741 cgtgaacgag tttccaggag aattctaggg gtcccgctat cgaaacccta agatgcttcg
     7801 agtcgccagg agcaatactg ccttgcgcct caaccacccg aaccttcttc cagggcggtg
     7861 tcaatcgctc gaagacgcag ggattgtcat gccatgcgag aacatcggtc ggggctgctg
     7921 ggacgcggga gcgataaacg cgcgtgattg tgctcatgag tatcacctct ggtggaaggg
     7981 ctttggttgg gaagccccgg cggaaaagag gaatgagaaa cgtggtagtc atcgcactac
     8041 ctggcagatg cgtcgcgcca cttcagtggt tcatatctgg cacccggcct gtatttcgct
     8101 ggtggcaccg cgggcccgtc gatcagcatc ttatcgatca ctgtcgtgaa tataaatcta
     8161 cagtataaat tcagatttgt caacccgggc aatgacgcct aacggaccgg ttctcccacg
     8221 ttgcgccccg cgccgaatga cgattagact cgcccgacag ccaggaggtt caccatctgt
     8281 tccaggcggc accgactacg tatcagtgat gtcttggcgt ctagataagg gagatcgcag
     8341 gttgcgaatc tgggacgttc cacccgccta tctctgtcgt cgacatctcc ttggcgagca
     8401 ccgggaactt cacgctattt ggtccgtgct cacccaggat aagactggat acgccaatca
     8461 tccggaaacc cgtcgatggc gcggaaagct ggcagccctc tttcaccgcc acgagttgct
     8521 catagccgaa atggaccggc gagggtacaa ccatcgcagc ccaattgatc gtgcattggc
     8581 caccggcagc catgcccaaa ccgactatgt cgacccaccc gacgagcagt acaggattct
     8641 gagacaaaaa ggttgtgatt gcggcaaagc gattcccaac accgtttagg gcgccccgtg
     8701 agccgcccgg tcatatgtcg cagctcgcca gtcttgtaga tctaacgcca gattaca
//
LOCUS       DASYWM010000077        21176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_302698, whole genome shotgun sequence.
ACCESSION   DASYWM010000077 DASYWM010000000
VERSION     DASYWM010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 21176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21176
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302698"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            438..848
                     /locus_tag="VGR29_03115"
     CDS             438..848
                     /locus_tag="VGR29_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2072610.1"
                     /translation="MFQQDEVRFGLTLKSLRETRRVSQSKLAERAGFDHSYVSRLESG
                     ARTPTRDAVQQLARALDLERVQQDELLAAAGFLPREVSSLLSNEPEITEVLGLLRNDQ
                     VPEAYRDSMRQVLRLLAEQARLVLKDDSSSVVVA"
     gene            1087..4605
                     /locus_tag="VGR29_03120"
     CDS             1087..4605
                     /locus_tag="VGR29_03120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV2072611.1"
                     /translation="MVDFAHLHLHTEYSLLDGMGRIGQYIDRAQALGMQHMAVTDHGV
                     MYAAMDWYTSATKAGIHPIIGMEAYLAEGPASRRERKSYHLLLLAENDTGYRNLLQLA
                     SKAALHGFYYRPRIDMEMLQEHREGIIATSACLGGPVANNFLHHQPEKAVEYAQALTD
                     IFGQERFFIELQDHGLKEQLDVNKQLIPLARKMNLPLVATNDVHYCCEEDAPAQDVLV
                     CVQTNTTINDSKRLKQESEQFFLKSPGEMAALFDEVPESITNTIRIAEMCNLDLGFKG
                     YHLPEYDVPDGTTTDRYLRQLCEDGARRHYGTLEGEIGTRLDYELSVIASMGFTNYFL
                     IVWDFVNFAKQNGILVGPGRGSAAGSLVTYCLGITGLDPLKYGLIFERFLNPSRISMP
                     DIDIDFADDRRGEVIDYVVQKYGDDRVAQIVTFGTLKAKAAVRDVGRAMGLSFGETDR
                     VARLIPTDPKMTIDKALGDVPELSKIYEGEAPVRDLIDTARKVEGLARHSSTHAAGVV
                     ISPDSLVHHVPLQRAGGKSEGDITTQWAQNHLEDLGLLKMDFLGLKTLTVLGRAVELA
                     RRSGSNITLEEIPMENEVAFELLKRGETFGVFQLEGGMTTRMTIDVAPESFEDLIALM
                     ALIRPGPMELAPDYIARKHGRVETEYQHPLLKDVLEETYGIALYQEQVMQIANVLSGF
                     SMAEADGLRKAMGKKLADEMAKYRDRFISGAKSTNNIEKKLAADIFDTIERFAGYGFN
                     KSHSAAYAVIAAQTAYMKANHPVEFMAALMSTEMGNTEKTVYNVSECRRAGVAVLPPD
                     ITRSGVEFSVETLEDGKKAVRFGLGAVKNVGVGAISAIVNARDQSDSGQLASLDAFCD
                     AVDWSSTNKRVIESLTKAGALDVYGHRAAVLAGLEQAAAAAQKRQKAAARGQMDLFGL
                     MVSDGVVVEGPSRLPEVPAAESRQILEWEKEFLGLYLSSHPLIAIIGDGLPDDYTQVV
                     EVSERFLNDRVRLVGMVKGVRRITTKTSKTMAIIEVEDLTGTIEMVAFPETFEKICDE
                     LEDDAILLFTGKVDERGEKRQIIIESASSSLPELNVKPKSRLTVMIELPLCGDYWEDV
                     SLMHKVDEVLRVNSGDAEVAFLVPHGGKVTRLRSRSRKITWTPEVAEELEAVLGGNRA
                     KIVQSLPNGSGPLLREAQEEREAVVV"
     gene            complement(4676..5917)
                     /locus_tag="VGR29_03125"
     CDS             complement(4676..5917)
                     /locus_tag="VGR29_03125"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV2072612.1"
                     /translation="MIVTDPAVPINGTLPLFNASFRTWQRDTGSDGLLPIIGISGTRG
                     KSTVLRLLEAMLNDTHLRSATWTDQGVQIRGRRQPGELSGWTHALTRLSEGSLDVALQ
                     ELDWATVNAVGLPQSSYPVMALTNLRERASSEEYSHSLQSAMRAAQQVVSAVADDGFI
                     VVNADDHYAVNAVADTDATVLYVALSHLSPSLHDHLEDGGTGVWVQQGSVMLGGRHRA
                     IRLCNVRDIPFTMGGEASFNVTNALIAVGLACGLGIDLPCVIRSLRAFRSTWGILPGS
                     MNTYETGEWRAAVDQIGPAWVLRPVLRAINPGASRRQVSVIGDLGAVDAEEVQELGRL
                     LGRFHGAIVLHSEQDERLVELFRHGLASNPYPPLLIRLPTERRAINRAFKALKSGDVL
                     LVLASGDSSAVHRAIRRHIPE"
     gene            complement(5914..6546)
                     /gene="rdgB"
                     /locus_tag="VGR29_03130"
     CDS             complement(5914..6546)
                     /gene="rdgB"
                     /locus_tag="VGR29_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEV2072613.1"
                     /translation="MPATEPVDIVVATGNLGKLAELREMLGPGYRVVSAAEAGAEMPN
                     ETGETFAENAKLKARAVAEQTGKIAIADDSGLEVSALGGSPGVRTARYAGAAATDAQN
                     REKLLKVLQDVGELERQARFVSVIAIALSPEEIITTRGICEGTIALEERGEGGFGYDS
                     IFQTPSGKTMAELEPAEKNRISHRGQAMRMATRFLADRLHVSGSPPEVHG"
     gene            complement(6564..7361)
                     /locus_tag="VGR29_03135"
     CDS             complement(6564..7361)
                     /locus_tag="VGR29_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV2072614.1"
                     /translation="MISEPSPLLPLVDTHCHLDDVSFNGDLADVLQESRDAGVPAWII
                     VGFAPERWDAAISMSRETPGMGHMLGVHPGHAEEWNDETRESLVSALVGSRARAVGEI
                     GLDFYRDNASYETQRRALVEQMHIARELDLPVVFHLRNAEEQMLETLEREHLLPRMVF
                     HSYDGTDRLTRFINDCGAVVGVGGLATRQKSASLREQLRKIPLSSMVLETDAPYLVPA
                     RQKVRRNTPAHLRIVADFLADHLGVAIEEVARETTSTAESVFGRLRP"
     gene            complement(7373..9127)
                     /gene="glmS"
                     /locus_tag="VGR29_03140"
     CDS             complement(7373..9127)
                     /gene="glmS"
                     /locus_tag="VGR29_03140"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV2072615.1"
                     /translation="MCGIFGYVGTSAPIVPMVASALRTMEYRGYDSWGIGWDDGVRFH
                     NRKAPGRVPRVIDASPQSGLAIGHTRWATHGVVSAENAHPHFDGAQQVGVVHNGVIEN
                     ATELMVSLGGTANFLSETDSEIVVHLVVRELEYGKTFSNAVEAVFLQLTGSNAIVVAN
                     RATGEIAAITSRSPLRLGRRGSSLYLASDPLALADIADEMAEIPDHTLVTMSRSSALL
                     RDILLGEEVAPTWVPVPADAHVEVGEHAHFTLKEIHDQPDLLMRLSAREGDVTPLAEV
                     IASHSHLVLTGCGSAYYAATLGAEWLRQRLNDTWVEAIPASEIAATTRNHGSKTLVLA
                     LTQSGETADVVDVLHIARSWGATTAALVNTESSTVASHVDIAVPLLAGTERSVLATKS
                     FFAMVMRQLQLVRAVDGIPTSWDDPFMEPVSSAISHWLSCETLNELARQLEVADHVLT
                     LGKASGHKVALEAALKIKEGSYVHAEAFLTGELKHGPLALVSQGTPCLLFATTGDEVA
                     SARIASREIMSRGGFTVGIGPFDPPDCSLVLWVADLGAAASLVHIVIAQRLAYLIAVR
                     RGVDPDFPRNLAKSVTVR"
     gene            complement(9270..9398)
                     /locus_tag="VGR29_03145"
     CDS             complement(9270..9398)
                     /locus_tag="VGR29_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072616.1"
                     /translation="MTRWESIETFGGWFPGSTVVMTIPNRTGNGPTIGRQRASSGI"
     gene            9585..10898
                     /gene="purB"
                     /locus_tag="VGR29_03150"
     CDS             9585..10898
                     /gene="purB"
                     /locus_tag="VGR29_03150"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV2072617.1"
                     /translation="MIERYTRPQMGAIWSQEHKIQQWLAVEIAVSEAWARRGRIPAEA
                     MEKIRDAHCDLDRMREIERETDHDVIAFLRAVGETVGDASRFIHLGLTSSDVVDTAMG
                     MQARDAADLLLEDLGNLLEALGQRAVEHKDTITVGRSHGMQAEPTTFGLKLAVWYDEL
                     RRQRERLQMARDHIAVGQISGAVGTHAHVPPSLEEEVCADLGLDVELVSTQVVQRDRH
                     AFYLAVLAGIAGTLEKMTVEVRHLQRSEVAEAEEAFGEGNQGSSAMPHKRNPHESERI
                     AGLARLVRGYASVGFENVALWHERDISHSSAERVIFPDACIVLDFMLYETAEIIRDLV
                     VYPEKMLANLNMSGGLIFSQRVLLALVDGGMDRQDAYRLVQRHAHSASVTGDTFRESV
                     AGDPEVQQRITVEQLETIFDPWDQLHNINATFQRVGLLDGEPHLE"
     gene            10891..11781
                     /locus_tag="VGR29_03155"
     CDS             10891..11781
                     /locus_tag="VGR29_03155"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV2072618.1"
                     /translation="MNSGTAPQLQHYPLLRRGKVRDIYDAGEALLLVASDRLSAFDVV
                     LPTTIPGKGRTLTSISEYWFEETRSLCPNHLVTTDISGLQLSPSEADALRGRCTVGRK
                     ADRIDIECVVRGYLAGSGYKEYRDYGTLAGESLPGGLRRGDALPDPRFTPAIKNDEGH
                     DENISRDRLAQLIGSDLAARLEETSMAVFHLASVRAAKAGLVLADTKLEFGWIGNEIA
                     IIDELLTPDSSRYWDASAIAPGEEPPSFDKQIVRNWLESQPWDKNPPGPEIPGEVVMV
                     TRERYEEVYRRLRDIKEEEE"
     gene            11786..12061
                     /gene="purS"
                     /locus_tag="VGR29_03160"
     CDS             11786..12061
                     /gene="purS"
                     /locus_tag="VGR29_03160"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEV2072619.1"
                     /translation="MAALQQRWIVEVEVMPKRGVNDPQGDAVLSGLRQLGFAEADRVR
                     VGKLIRLDITAKNEDEARSTGQRMCERLLANPVIEEYELVVHANDQK"
     gene            12074..12766
                     /gene="purQ"
                     /locus_tag="VGR29_03165"
     CDS             12074..12766
                     /gene="purQ"
                     /locus_tag="VGR29_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEV2072620.1"
                     /translation="MHVGVISFPGSNGDHDALHNLESDLGVQVRRVDYRETSLTGLDA
                     VVLPGGFSYGDALRCGAIARFAPIMTHLQAAAQDGMPILGICNGFQILCEAHLLPGAL
                     LRNRTLRFHCFWTYIRVERQDSSWTSNLTEGEILRLPLAHGDGAFYVGDRQLAELEEN
                     GQVVARYCTAEGAVTEDANQNGSVANIAAICNEQRNVVGLMPHPERATNELVGGGDGL
                     KLLRSLLLAVPA"
     gene            12817..14337
                     /locus_tag="VGR29_03170"
     CDS             12817..14337
                     /locus_tag="VGR29_03170"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEV2072621.1"
                     /translation="MSEEQFQKLLRRVQQVADIGGAAALLSWDQRTLMPPLGRPVRAE
                     RLATLAGLAHQIFTSDETGALLEQAQPHVESFSEESFERSLWRLVKRNYDKLKRIPTE
                     LSQDLQRAGNLGYEAWHTSRASSDYSRFHPALARNIDLNKQVLDIHRGAIPDIEDHYD
                     ILLDDYEPGMKTSDITRVFDRLKEATKPLVQQVVENGGKGRDRLVHGHFPTRKQDALV
                     RSVVQQLGFTDDAWRLDVTPHPFASAMGTSDIRITTKFEEDFINPAFFGTMHEFGHGL
                     YEHQVSPDLERTPLARGASMMWHESQSRMWENLVGRGRPFWTWAMPKVQDVFPDQFGA
                     ADADEMYRAVNAMGPSLIRIEADELTYNLHIIIRFELERDLYSGAVSPADLPEAWNTK
                     IREYLNIDVPNDAQGVLQDVHWGSGSFGYFPTYALGNVLGAMLWERILTENPGIEGSF
                     ENGEFGQLREWLRENVHRHGRKYMPMDLLEKVLGVRQIEAEPLVSYLTNKVNELYP"
     gene            complement(14399..14914)
                     /locus_tag="VGR29_03175"
     CDS             complement(14399..14914)
                     /locus_tag="VGR29_03175"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV2072622.1"
                     /translation="MEQLLYIAIAISAGTINALQLAMIGGLTRQRGGFESTFISMLAS
                     VGGLAVILGGMLLLGKNLSLPEVFRSPWSFLALAIVMSILLLAAAHGLPPYYLLTGLT
                     AVPYLLAAAFVGPRLGLGVYFAAIISGQMAGSLLFDHIGAFGAGTRPVDAARLAGVVL
                     LVVGVVLIRGR"
     gene            complement(15421..16410)
                     /gene="fmt"
                     /locus_tag="VGR29_03180"
     CDS             complement(15421..16410)
                     /gene="fmt"
                     /locus_tag="VGR29_03180"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV2072623.1"
                     /translation="MSSRIIYMGTPNFAVPAMERVALGDSGDLALVVTQPDRAAGRGR
                     KHVAPAVKTAAERLGVPVLQTATLRDPEVRQRIIDIRPDLIIVAAFGMILGRWILELP
                     ARGCVNLHASLLPKYRGANPIAAAIAQGEEVTGVSLMQMDRGLDTGGVYATTEIDIEL
                     DDTTESVTQKLANRAGMLLEDNVSALLDGSLLPVPQGEGATLTRQMTKADGWLDFVRP
                     AVEMERHIRATWPWPRAWTTDPAGERIQVHAASVSDEFALHPGQIAHADGQVVVGTGR
                     GSLILRRVQLPGGRPIDDSAIMQVRSLSDGQVLGVVDRPEDLPPLVIPVPNDG"
     gene            16514..19219
                     /locus_tag="VGR29_03185"
     CDS             16514..19219
                     /locus_tag="VGR29_03185"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEV2072624.1"
                     /translation="MPVYVAALPSFQVEGVDPSERDQPLTFDQPVGELPIVDDARWSS
                     SVTDNSLTALLVWIVLLIVLQAVTWPLVRRVFSRFPDKGWAFTRLTTILLAGYGVWLL
                     SSIQLIAFRAIWAAAALLVLAAASWLAVRRITTGRDNGSWWRNPVIIVAEAVFWASFA
                     LFLTYRFINPDSYHPIWGGEKPMEFAHLNAILRSAHFPPVDPWYSGGYINYYYYGTYL
                     VAFLIKLTGIPVEIAFNLAQPTFPALLAASSFSLSAALGKRLTRSAGGAITSGLIGAF
                     FVQFAGNFIVAERLIDRARNGLGPVNGFSYWVFDPTRAIPDAARPINITEFPYFGALY
                     ADLHPHVIAMPFTILTVALAWQIASQWRILPVALARRSAAREARFALLAPLALAVVGL
                     GTLFMTNAWDLPMYAAIVGVAVIMATRGIPRIVPRLHVSLTLLFVLGVTTWLLILPFT
                     SHYVALFGEIETVRDRTPLLAIESHLGGQILLMTIGIQALVASGLRFPRPAVTPYAVA
                     SIYLVAIFLFMQSGSNEGSEIWNRAGELGTVAVVVGMWIIAAWSATRARTEFGMHPAA
                     LKTLLVEVAAITVALLVLDRLVLALYLGIGLSAAILWFSLRRPGERFLVALIAGGSLL
                     GAALEIVYLVDDLSGGDWYRMNTIFKFYNEIWNLFGIATGVLAGHAVWAATRTRRRLN
                     STVGQRTYPNVSVWARATTIILIPVVIASLAYAVVATPIRLEQSWGERNEPTLDAYQW
                     MEYASMPLQQGGALTYQDDLAAINWFNEKVPGTPVIAEAAFGPYRCNGSRYSIATGLP
                     AVIGWQRHEQQQRYLEDLGSREAALRELYTTPSADVKREIIERYGIEYIIVGETERHY
                     PTVNGNDCIDTGSPEGIAALESMAGRFLEVAFQQGTTTVYRVTGD"
     gene            19290..19363
                     /locus_tag="VGR29_03190"
     tRNA            19290..19363
                     /locus_tag="VGR29_03190"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19324..19326,aa:Pro,seq:tgg)
     gene            complement(19439..20038)
                     /gene="rpsD"
                     /locus_tag="VGR29_03195"
     CDS             complement(19439..20038)
                     /gene="rpsD"
                     /locus_tag="VGR29_03195"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV2072625.1"
                     /translation="MNFTGAKVKKSRALGVALTPKAQKYMKNRPFPPGQHGQRRRRRP
                     SDYGVQLLEKQRLRFQYNISEKQLARAYATASRMPGSTGDNLMQVLETRLDALVLRAG
                     FAPTIYAARQYVTHGHFMVNGKKASIPSMRLKPGDVITVRDKSRKSVIFGDLLARGPV
                     APYMTFDENNLSARFDYVPPREEIPILCAEHLVVEYYSR"
     gene            complement(20302..20715)
                     /locus_tag="VGR29_03200"
     CDS             complement(20302..20715)
                     /locus_tag="VGR29_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072626.1"
                     /translation="MNEDMRRMHRPADMSPDNGEHEPLGEDQGVGVRFSADQVATAFE
                     VDVNRVHNAFSGEYGLDADGMVDSRQAQELVELIIGDRPQAEREEALITLGAFTPRSD
                     TAEPSVFEKPPGELSDRLRPSEQQPNVTAPKDADQ"
BASE COUNT         4430 a         6221 c         6211 g         4314 t
ORIGIN      
        1 gttggaaaag ataggagctc ggaaggttgg gggcagcgac aacgctaagg aaccaggcgt
       61 gtcccccgag gcgcagtata gcagcctcgg tccgctccac gaggccccag gagccccgct
      121 gaccgccgtt gttagtcgga agtacttacg gccccaacgt cctcacgccc caggttcacc
      181 ccaattgaca gcatatcgac agccgatgtc gtaacgatgg cctatgctct gcccctacga
      241 cgttactgtc gttgatcgca tggcaaaacg caggtcgagt ggacagaccg gaaaggctgt
      301 tgacattgag gcaacccggg ggtaacatct attcacgcgg ggcgcgacgg ttgccggtta
      361 ggcaactggg ctagccgggg aggttgtgca aatgatgtgc acattccttt tgtctaggga
      421 caggagaggt tggggaaatg tttcagcagg atgaggttcg gttcggatta accctgaaga
      481 gcttgcggga gacgcggcgc gtctcgcaga gcaaactggc agagcgtgcc ggtttcgatc
      541 attcctatgt gagtcgtctg gaaagcggtg cgcggactcc gacccgcgac gcggtgcagc
      601 agctcgctcg cgcgctggat ctggagcggg ttcagcagga cgaactgctc gctgcggcgg
      661 gattccttcc ccgtgaggtc tccagccttc tgtcgaatga accggagatt accgaagttc
      721 tggggctact ccggaacgac caggtgcccg aggcctatcg cgactcgatg cgccaggtcc
      781 tgcgactgct cgccgaacag gcgcgattgg tgctcaagga tgattcatcg tcggtggtgg
      841 tggcctaaag cgacatatcc ctaggagctc caatcaccat cacgtccgtc cccgccacgc
      901 ggcctatcga tctatggcca gatgctgcat actggctggg tgatcgcaga cgataacgtc
      961 ggtgcgagct aacaggcttt gttcttaccg ccggtccact ccccgcaacc tgaccggcag
     1021 cgtcgcaatg cgtccccgcc agtgaaacaa caacatgtcg gtccgacacc cagaggaact
     1081 gcttccatgg tcgatttcgc gcacctgcat ttgcatacgg agtactcgct gctcgacggg
     1141 atggggcgta ttgggcaata cattgaccgg gcccaggcgc tggggatgca gcacatggcc
     1201 gtgacggatc acggggtcat gtacgcagcg atggactggt acacctcggc gacgaaggcg
     1261 ggaatacacc ccatcatcgg catggaggcg tatctggccg agggaccggc gtcaaggcgg
     1321 gagcgtaagt cctatcatct tctcctgctc gccgagaacg acacgggata ccgaaacctg
     1381 ttgcaattgg ctagcaaggc tgcgctgcat gggttttatt accggccccg catcgatatg
     1441 gagatgctcc aggaacaccg ggaagggatt attgcgacgt ctgcatgcct gggtggaccc
     1501 gttgccaaca acttcctgca tcaccagcca gagaaagctg ttgagtacgc acaggcactc
     1561 accgacatct tcgggcaaga gcgattcttt atcgagctgc aggaccatgg tctaaaggag
     1621 cagctggacg ttaacaaaca gttgattcca cttgcccgga agatgaacct gccgctcgtc
     1681 gcaaccaacg atgttcatta ttgctgtgaa gaagatgccc cggcgcagga cgttctcgtc
     1741 tgcgtgcaga caaatacgac catcaacgat tccaagcgcc tgaagcagga gtcagagcag
     1801 ttcttcctca agagccctgg ggagatggcc gccctgtttg atgaggtgcc tgagtcgata
     1861 accaacacaa tacgcatagc ggagatgtgc aacctcgacc tcggcttcaa gggctaccac
     1921 ctcccggagt acgacgtccc cgacggcaca acgacggacc gatatctccg tcagctgtgc
     1981 gaggacggcg cccggcggca ctacggcaca ctggaagggg agatcggtac acgactggac
     2041 tatgagctgt cggtaatcgc ttcaatgggc tttacgaact acttcctcat cgtgtgggac
     2101 ttcgtcaact ttgcgaagca gaacggcatt ctggtcggcc ccggtagagg atcagccgca
     2161 ggaagtctcg tgacctactg ccttggcatc accggtctcg atccgttgaa atacggccta
     2221 atctttgagc gattcctcaa tccttcccgc atctcgatgc ccgatattga tattgatttt
     2281 gccgatgacc gccgaggcga ggtgatcgac tatgtggtcc aaaagtacgg ggacgacagg
     2341 gttgcccaga tcgtcacgtt cgggacactc aaggcgaaag ctgcagtgcg cgatgtcggt
     2401 cgcgccatgg ggctgtcctt cggcgagacc gaccgcgttg cccgcctgat tcctaccgac
     2461 ccaaagatga ccatcgataa agccctcggg gatgtgccgg agctaagcaa gatctacgaa
     2521 ggtgaggctc ccgttcgtga cctgatcgac actgcccgca aagtcgaggg gcttgcccgg
     2581 cactcgtcta cacacgccgc gggcgtggtt atctcgccgg attccctggt gcaccatgta
     2641 ccgctacagc gggctggcgg caagagtgag ggagacatca ctacacagtg ggcccagaac
     2701 catctcgagg acctggggct gttgaagatg gacttcctcg gcctcaagac attgacggtg
     2761 ctcgggcggg ccgtggagtt ggcccggcgt tcggggagta acatcacgct cgaagagatt
     2821 ccgatggaga acgaagtggc attcgaactg ctcaaacgag gagagacatt cggcgtcttc
     2881 caactggaag gtggcatgac gacgcgtatg acgatcgatg tcgctcccga gagcttcgag
     2941 gacctcatcg cactcatggc tcttattcgt cccgggccga tggagcttgc gccggactac
     3001 atcgctcgca agcacgggcg agtggagacg gaataccagc acccgttgtt aaaggacgtc
     3061 ctggaggaga cgtacgggat cgctctctac caggaacagg tgatgcagat cgcgaacgtc
     3121 ctgtcggggt tctcgatggc agaggccgat ggcctccgca aagcgatggg caaaaaactc
     3181 gcggacgaga tggcgaagta ccgcgaccga ttcatctctg gcgcgaagtc gacgaacaac
     3241 atcgagaaga aattagcggc cgacatattc gatacgatcg agcggtttgc gggttacgga
     3301 ttcaataaat cgcatagtgc agcctatgcg gtgattgccg ctcagaccgc gtatatgaag
     3361 gcaaatcatc cggttgagtt catggcggcc ctgatgtcca ccgaaatggg caacaccgaa
     3421 aaaaccgtct acaacgtttc cgagtgccgg cgagccgggg ttgcagtgct gcctcccgat
     3481 atcacaagga gcggagtgga gttttctgta gaaactttgg aggacgggaa gaaggcggtg
     3541 cgctttggtc tcggtgccgt aaaaaatgtt ggtgtcggag ccatttcagc gatcgtcaat
     3601 gcacgagacc agtcggactc ggggcagctc gcgtcactgg atgccttttg tgacgccgtc
     3661 gattggtcct ccaccaacaa acgagtcata gagagcctca ccaaggcggg cgcgcttgac
     3721 gtctatggac accgggcggc agtgctggca ggtctggagc aggcggctgc ggctgcccag
     3781 aagcgacaaa aggccgcagc tcgggggcag atggacctct tcgggctgat ggtttccgac
     3841 ggagtagtgg tggaaggacc cagtcggctc cctgaggttc cggccgcaga aagccggcag
     3901 atcctggagt gggaaaagga attcctggga ctctatctga gctcgcaccc gctgatcgcg
     3961 atcatcgggg acggcctgcc ggacgattac acgcaggtgg tggaagtctc ggagcggttc
     4021 ctgaatgacc gggttcgcct cgtcggaatg gtgaagggag tccgccgcat cactaccaaa
     4081 accagcaaga cgatggcgat catcgaggtt gaagacttga ccgggaccat cgagatggtt
     4141 gccttcccgg agaccttcga aaagatctgc gacgaacttg aggacgacgc tatcctgctc
     4201 tttacaggga aagtggatga gcggggagag aagcgccaga tcatcataga gagtgccagc
     4261 agctcacttc cagaactcaa cgtcaagccg aagagcaggc taacggtcat gattgagctt
     4321 ccgctgtgcg gggattactg ggaagacgtg tcgctcatgc acaaggtaga cgaagttctc
     4381 cgggtaaata gcggagatgc ggaagtcgcg ttcttggttc ctcatggagg gaaagtgact
     4441 cgccttcgga gtcgtagccg caagataacg tggactcccg aagtcgccga ggagctggaa
     4501 gcagtactgg gagggaaccg tgccaagatc gttcaatcgt tacccaacgg aagcggaccc
     4561 ctgctgaggg aagcacagga ggaacgcgaa gcggttgttg tgtagcaata tgctcgcacg
     4621 acatatccca ttgagatcat acgcctactc cgagccgctg ttgtgcagat tgtagttact
     4681 cggggatatg tcttctgatg gcccgatgca cggcagagga atcgccgctc gccaagacca
     4741 gaagcacatc gccactcttc aacgccttaa acgctcgatt gatagcacgt cgctcagtcg
     4801 gcagtcggat caacagaggc gggtagggat tcgaagcgag cccatgccgg aacaattcca
     4861 ccaagcgctc atcctgctcc gaatggagta cgatggctcc gtgaaatctc ccgagcaagc
     4921 ggccgagttc ctggacctct tcggcgtcaa cagcgcctag atctcctatc acgctcacct
     4981 ggcgtcggga agcgccgggg ttgattgccc gcaaaactgg acggagcacc caggcaggtc
     5041 caatttggtc gacagccgct cgccactccc cggtttcata ggtattcatg gagccgggga
     5101 ggatacccca ggtagaacga aacgctcgca gggaccgtat tacgcagggc aggtcgatcc
     5161 cgagcccgca tgccaggccg acggcgatca atgcattggt gacattgaac gacgcctcgc
     5221 cacccatcgt gaacgggatg tcccgtacgt tgcacaaccg gatcgctcga tgccgaccac
     5281 cgagcatgac ggaaccctgt tgtacccaga ccccagtacc gccgtcctcc aggtggtcgt
     5341 gaagacttgg tgagagatga gacaaggcga catagagcac cgtcgcgtcc gtgtcggcaa
     5401 cagcgttgac cgcatagtga tcatccgcgt ttacgacaat aaatccatcg tcagcaactg
     5461 ccgacactac ctgttgcgct gcgcgcatcg ccgactgcaa ggagtgagag tattcctccg
     5521 acgatgcccg ctctctgagg ttggtgagag ccatgacagg gtaggagctt tggggaagcc
     5581 ccaccgcgtt gaccgttgcc cagtcgagtt cctgaagggc aacatcgagc gatccttcgg
     5641 aaaggcgcgt caaggcgtgc gtccagccgg aaagctcccc aggttgtcgg cggccgcgta
     5701 tctggacgcc ctggtctgtc catgtcgctg atcgcaagtg cgtatcgttc agcatcgctt
     5761 caaggaggcg aaggactgtg ctctttcctc tggtcccgct aatcccaatg atgggcagca
     5821 atccgtcgct tcccgtatcc cgctgccagg ttcgaaagga agcgttgaaa agcggcaagg
     5881 tgccgttgat aggaacagcc gggtcggtaa cgatcatcca tggacctccg gcggtgaacc
     5941 tgagacgtgt agccgatcgg ccaggaaccg ggtggccatc ctcatcgcct gaccccggtg
     6001 gctgatccga ttcttttcgg ctggctcaag ctcggccatt gtcttcccgc tcggcgtttg
     6061 gaatatggaa tcgtagccga accctccttc acctcgctct tcgagcgcga tggtgccctc
     6121 acagatgcca cgggtcgtga taatttcttc tggcgaaagg gcgatcgcga tcactgagac
     6181 aaaacgagcc tgtcgctcaa gttcgccgac atcctggaga accttgagca acttctcccg
     6241 attctgcgcg tcagtcgctg ctgctccggc atacctggca gtgcgtacgc ccggagagcc
     6301 gcccaaggcc gacacctcca gacccgaatc gtccgcgatg gcaatctttc ccgtctgctc
     6361 agctacagca cgtgccttta gctttgcatt ctcagcgaac gtctcgccag tctcgttagg
     6421 catctcggct cccgcctctg cagcgctcac gacccggtat cctggaccga gcatttctcg
     6481 cagctcggcc agttttccca agttcccggt ggctaccacg atgtctacag gttccgtcgc
     6541 cggcattccc ttaagattcc tcctcacggc cgcagccttc caaacaccga ttcggccgtc
     6601 gatgttgtct cgcgagcgac ctcctcgatt gcaacaccaa ggtgatctgc aagaaagtcg
     6661 gccacgatac ggaggtgcgc cggagtgttg cgccggactt tttgacgagc gggaacgaga
     6721 tatggcgcat ccgtttccag gaccattgag ctcaacggta tcttccgaag ttgttcccgc
     6781 aggctagctg acttctgacg ggtagcaaga ccgcccacgc ctacaacggc gccgcagtca
     6841 ttaataaatc gagtcagacg atcagtgccg tcatacgaat gaaagaccat tcgtggcagc
     6901 agatgctcgc gctcaagagt ctcgagcatc tgctcttctg cgttcctcaa atgaaagacg
     6961 actggcagat ccagttcgcg agcaatgtgc atctgttcga ccagcgctcg gcgctgagtt
     7021 tcgtaggagg cgttgtcccg atagaagtcc agacctatct ctccaacggc ccttgcacgg
     7081 gaaccaacga gggcgctgac cagactctcc cgggtctcgt cgttccactc ctcggcatgg
     7141 ccgggatgaa ccccgagcat atgacccatt cccggggttt cgcgggacat actgattgct
     7201 gcatcccacc tctcaggggc gaagccaaca atgatccagg ccggcacccc tgcgtctcga
     7261 ctctcttgca gcacatccgc gaggtcgcca ttgaaggaga cgtcgtcgag atggcaatga
     7321 gtgtctacca gtggcagcaa tggtgatggc tctgatatca cattgatccc gtctaccgaa
     7381 cagtcacact tttcgctaaa ttccggggga aatccggatc gacgccgcgc cggacggcga
     7441 tcagatacgc aagccgctgg gcaatcacga tatgcaccag tgatgccgcg gctcccagat
     7501 cggcgaccca cagcacgagc gagcaatccg gcggatcgaa cgggccgatc ccgaccgtga
     7561 aacctccgcg gctcatgatc tccctggagg ctattcttgc ggacgcgact tcatctccgg
     7621 ttgttgcaaa cagtaagcaa ggggtccctt gcgaaacgag cgcaagcgga ccatgtttca
     7681 gctctcccgt aaggaaagcc tcggcatgaa cgtaacttcc ctccttgatt ttcagggccg
     7741 cctcgagagc aaccttgtgt ccagatgctt tccccagcgt gaggacatga tccgccacct
     7801 cgagctgccg cgcaagttcg ttcagcgttt cacaagagag ccaatggctg attgcgctcg
     7861 acaccggctc catgaatggg tcatcccagg acgttggaat accgtccacg gcacgaacca
     7921 gttggagttg acgcatcacc attgcaaaga acgatttggt cgccagtacg ctacgctctg
     7981 ttcccgcaag taacggaacc gcgatatcga cgtgactcgc tacggtcgag gactccgtgt
     8041 tgactaatgc agcagtcgtt gcgccccagg agcgagcaat atgcaacacg tcaacgacat
     8101 ccgcggtctc cccgctctgg gtcaatgcca ggacaagcgt cttcgacccg tggttcctgg
     8161 tcgtagcagc tatttcagat gcaggtatgg cctcgaccca cgtgtcattc aaccgttgtc
     8221 gtagccattc cgctcctaat gtggcggcat aataggccga cccgcaacct gtaagaacca
     8281 ggtgcgagtg ggacgcgatc acctcggcga gcggcgtaac gtcgccctca cgggcggaga
     8341 gccgcatcag cagatcgggc tggtcgtgga tctccttaag ggtgaagtgg gcgtgttctc
     8401 ctacctcaac atgggcatcg gccggcacgg gaacccaagt tggggcaacc tcctcaccaa
     8461 gcagaatatc ccggagcaac gctgatgagc gggacatggt gacgagagtg tggtcaggaa
     8521 tctccgccat ttcgtctgcg atatctgcaa gagcaagcgg gtcagatgcc aaataaaggg
     8581 aactcccacg tcgtcccagc ctcaagggtg accgggatgt gatggcagcg atttcgccag
     8641 ttgcgcggtt tgcgacaacg atggcattgc tccccgtcaa ttgaaggaag acagcctcta
     8701 ctgcattgga gaacgttttc ccgtactcaa gctcgcgcac gacgagatgg acgacgatct
     8761 cgctgtcggt ctccgagagg aagttggctg tgccaccaag tgacaccatc aactcggtcg
     8821 cattctcgat aaccccgtta tggacgacgc cgacctgctg tgcaccatca aagtgcggat
     8881 gagcattctc ggcgctgaca actccgtgcg tcgcccagcg cgtgtggccg attgcgagtc
     8941 ctgactgtgg agatgcatcg attacccgag gcacgcggcc aggcgccttt cgattgtgga
     9001 atcggacgcc gtcatcccag ccgatccccc atgagtcgta tccccggtac tccatcgttc
     9061 gcaacgcaga agcgaccata ggcacaatcg gggccgacgt tccgacgtag ccaaaaattc
     9121 cgcacatgag gttgttcccc ttgtcacgca agagcagagg ttctaccagg cgagacttgc
     9181 cgatacgcga gcgcgcgtcg acccctgccg gagaaggctc tcctcaaatt ttgattgatc
     9241 aagaggattc gtttgcagag gatagcggcc tagatgccgc tgcttgcacg ctggcgaccg
     9301 attgtcggcc cgttaccggt ccggtttggg atcgtcatga cgactgtgct gccggggaac
     9361 catccgccga atgtttcgat agattcccac ctcgtcaacc cgctctcctg cgtcgggcca
     9421 tggcgctgta cactcggtga acgaaccctg acatcgaatc catttgcatc atagcccttt
     9481 gacggcctat agcgcaatga agcgcacgta tacttacggc gagcagccag caggggcaaa
     9541 agggcgcctt ctggattccg gcatttcgag agcggagaca gcaggtgatc gaacgttaca
     9601 cgcggccgca gatgggcgcg atttggtctc aggaacacaa gattcagcaa tggctcgccg
     9661 ttgagatcgc tgtctccgaa gcctgggcca gacggggacg tatccccgca gaagccatgg
     9721 agaaaatccg ggacgcccac tgcgacctag accggatgcg ggagattgaa agagagacgg
     9781 accacgacgt tatcgcgttc ttgcgtgctg tcggtgagac cgttggagat gcatcccgat
     9841 tcattcacct cggcctgacc tcctccgacg ttgtagatac cgcgatgggg atgcaggccc
     9901 gcgatgccgc cgatctcctt cttgaggacc tgggcaactt gcttgaggcc ttgggccaac
     9961 gtgctgttga gcataaagac accatcaccg tcggccggtc tcacggaatg caggccgagc
    10021 cgacgacctt tggactcaaa ctggctgtct ggtatgacga gcttcgccgt caacgcgagc
    10081 gcctgcaaat ggcccgtgat cacattgccg tcggacagat ctcgggtgcc gtgggcaccc
    10141 acgcccatgt tcctccatct cttgaggaag aagtctgtgc cgatctcggt ctggacgtgg
    10201 agctcgtttc cacgcaggtc gtccagcgtg accgccacgc cttttacctg gcggtgctcg
    10261 cagggatagc cggcaccctg gaaaaaatga cggtggaagt tcgtcacctt cagcgcagcg
    10321 aagtggcgga ggcagaggaa gcgttcggtg agggcaacca gggctcatcc gccatgccgc
    10381 acaagcgtaa cccgcatgaa tcggagagga tcgccggttt ggcgcggctc gtccgagggt
    10441 acgcctcggt gggctttgag aatgtagcgc tctggcatga acgcgacatt agccacagct
    10501 cggctgaacg cgtcatcttt cctgatgctt gtatcgtgct cgatttcatg ctctacgaaa
    10561 cagccgagat tatcagagac ctggttgtgt atccggaaaa gatgctcgcg aatttgaaca
    10621 tgagcggggg cctgatcttc tcccagcggg tgctcctggc attggtcgat ggaggaatgg
    10681 accggcaaga cgcctaccgt ctggtgcagc gccatgccca ctctgcatca gtaacagggg
    10741 acaccttcag ggaatcggtc gcaggtgatc cagaggtgca gcagcgcatc actgtcgaac
    10801 aacttgagac aatcttcgat ccgtgggatc agttgcataa catcaacgcg acgttccaac
    10861 gtgtggggtt gctcgacgga gaaccacatc ttgaatagcg ggaccgcgcc ccaactccag
    10921 cattacccgc tgctgcgcag aggcaaggtc cgggatatat acgacgccgg tgaggctctc
    10981 ctgctggtgg cgagtgaccg tctctccgcg tttgacgtcg tcctgccgac tactatcccg
    11041 ggcaagggca gaaccctcac atccatctct gaatactggt tcgaggagac acggagcctt
    11101 tgccccaacc atttggtcac caccgacata tccggactcc agttatcgcc ctcggaggca
    11161 gacgcccttc gcggccgatg cacggtggga cggaaggcgg atcgcatcga catcgagtgc
    11221 gtcgtgagag ggtacctagc ggggtcgggg tacaaggagt acagggatta cggcacactt
    11281 gctggagaat cattacccgg ggggcttcga cgcggcgacg cgctccctga tcctcggttc
    11341 acccccgcga tcaagaacga cgaggggcat gacgagaaca tttcacggga tcgtctcgcg
    11401 cagctgattg gaagcgacct ggccgccagg ctcgaggaga cgagcatggc ggttttccac
    11461 cttgcatctg tgcgggctgc caaggcggga ctggtgttgg ctgataccaa gctggagttc
    11521 ggctggatcg ggaacgagat cgctatcatt gatgagctcc tgacgcccga cagttcccgg
    11581 tactgggatg caagcgcgat cgctcccgga gaggagccac caagcttcga caaacagatc
    11641 gtgcgcaact ggttggaaag ccaaccttgg gacaagaacc cgcctggccc cgaaattccc
    11701 ggggaagtcg ttatggtcac gcgtgagcgg tatgaagagg tctacagacg tctcagggat
    11761 atcaaagagg aagaggagta aatcgttggc agcattgcag cagcgctgga tcgtcgaggt
    11821 agaagtcatg cccaaacgcg gcgtcaacga ccctcagggc gacgctgtgc tctcagggct
    11881 gaggcaactc gggtttgcgg aggcggatcg ggttcgtgtt ggcaagctga tacggctcga
    11941 cataacggca aagaacgagg atgaggcgcg gtcgacggga cagaggatgt gtgagcggct
    12001 cctggcgaac cccgtgattg aggagtatga gctggtagtc cacgccaacg accagaagta
    12061 ggggagaaaa gcgatgcacg tcggtgtcat ctcatttccg ggtagcaacg gcgatcacga
    12121 cgcactccac aacctggagt ccgatctcgg tgtccaagtg aggcgagtgg actataggga
    12181 gacatcgctt accgggctcg acgcagtggt gctgcccggt ggtttctcct atggtgatgc
    12241 ccttcggtgt ggcgcaatcg cgcgattcgc gccaatcatg acccacctgc aggcggccgc
    12301 acaggacgga atgccgatcc tgggtatctg caacgggttc cagattctgt gtgaggcgca
    12361 cctgcttccc ggcgcgttgc tccgcaacag gacgctccgg tttcattgct tttggaccta
    12421 catcagggtc gagcgacagg actcgtcatg gacatccaac ctgaccgagg gggagatcct
    12481 ccggttgccg cttgcccacg gcgatggtgc gttctatgtt ggcgatcgac aacttgctga
    12541 gttggaagaa aacggtcagg ttgtggcgcg ctattgtacc gctgagggag cggtgacgga
    12601 ggacgcgaac cagaacggat ccgtggcgaa tatagccgcc atctgcaatg aacagcgcaa
    12661 tgttgttgga ctcatgccgc atcctgaaag agcgaccaac gagcttgtcg gaggtggtga
    12721 cggattgaag ctcctccggt cgctccttct ggctgtcccg gcctgagtgg gatcgctact
    12781 tgtaatatga cgaccactta attcacggag tacttcatgt cggaagaaca gtttcagaaa
    12841 ctcctcaggc gcgtccagca ggtcgcggat atcggcggtg cagccgctct gctcagctgg
    12901 gaccagcgca cgctcatgcc gccgctgggt cggccggttc gggcggagcg tctcgccaca
    12961 ctcgccggtc ttgcacacca gatattcacg agcgacgaaa cgggtgcgtt gcttgaacag
    13021 gcacaacccc atgtcgaatc gttctctgaa gagtcatttg aacggtcact ctggcgactc
    13081 gtgaagcgca attacgacaa actaaagcgg atcccgacgg agctgtcgca ggatctgcag
    13141 cgcgccggta atctggggta tgaagcatgg cacacctcac gagcgtcctc ggattattcc
    13201 agattccatc cagcgcttgc gcgcaatatc gacctcaaca agcaagtgct ggatatccac
    13261 cggggtgcga ttcccgatat agaggatcac tacgacatcc tcctggacga ctacgagccg
    13321 gggatgaaga catccgacat cacccgcgtg ttcgatcgcc tgaaggaggc caccaagccg
    13381 ctcgtgcaac aagttgtcga gaatggaggc aagggccgcg accggctggt acacgggcac
    13441 ttcccgaccc ggaagcagga cgcgctggtc cgcagcgtcg tccagcagct cggatttact
    13501 gatgacgcct ggcgcctgga cgtcactcca catccctttg cgtcggcaat ggggacgagc
    13561 gacatccgca tcaccaccaa gttcgaggag gactttatta atccagcctt cttcggaacg
    13621 atgcacgagt tcggtcatgg tctctacgag caccaggttt ccccggacct cgaacggact
    13681 ccgctggccc gcggagcatc aatgatgtgg cacgaaagcc agagccgcat gtgggaaaac
    13741 ctcgttgggc gcggacgacc attctggacc tgggccatgc cgaaggtgca ggatgtcttc
    13801 ccggaccaat tcggggctgc cgacgccgac gagatgtacc gtgccgtgaa tgccatgggt
    13861 ccgtcgctga tccgcatcga ggcagacgag ctgacctaca accttcatat catcattcga
    13921 ttcgaacttg aacgtgatct ctattcgggc gccgtctcac cagcggacct tcccgaagcc
    13981 tggaatacca aaatcaggga atatctgaat atcgatgttc ccaacgatgc gcagggcgtt
    14041 ttacaggatg tccattgggg ctctggttcg ttcggttact tccccacata cgcgctcgga
    14101 aacgtgttgg gcgccatgct ttgggaaagg attctgacag agaatcctgg catcgaaggg
    14161 agcttcgaga atggcgagtt cgggcagtta cgtgagtggc tgcgggaaaa cgtccaccgg
    14221 cacggacgca agtacatgcc gatggacctg ttggagaaag tcctgggcgt tcggcagatc
    14281 gaagctgagc cccttgtttc ctacctgacc aacaaggtta acgagctcta cccatagagt
    14341 ggcggaaaga ttggcataag gagtggccgg gagatcatcc cggccgcttt tgtagattct
    14401 atcgtccacg tattagcact acgccaacga caagcaacac gacacccgcg aggcgcgccg
    14461 cgtcaacagg tcgtgtaccg gcgccgaacg ctccaatgtg gtcgaaaagc aacgatccgg
    14521 ccatctggcc cgagataatc gcagcaaaat agacaccgag accgaggcgt ggtccgacaa
    14581 acgctgctgc cagaaggtac ggcacagctg tcaagcctgt tagcaggtag tacggaggca
    14641 atccgtgagc ggcggcgagc agcaatatgg acatcacgat tgcgagagcc agaaatgacc
    14701 atggcgagcg aaatacctct ggcagggaaa ggttctttcc aagtagcagc atccctccca
    14761 gaatgactgc gagaccgccg accgaggcaa gcatggagat gaaggtggat tcgaaccctc
    14821 cgcgctggcg tgtcagtccg ccgatcatcg ccagctggag agcattgatt gtaccggcac
    14881 tgattgctat cgcgatatag aggagttgtt ccacgatccg ggactccctg gagtttaaga
    14941 tttctttgcc ttcgaccgta tcactttgcc cagctagcat atggtgagcc gttcacgtcg
    15001 cccagagaca ctggagactt ccgtcgacgc cgaattccgg tgaatcattt tcattgggca
    15061 tggccacagt aggtctacct atcgcggtca tcgttgctca ctttgccatc aaagagccgg
    15121 aaagcgccag caacactttg ttaggcatta tgccggaaga cctttactcc acgtgcctca
    15181 gtgggcgctt acgcgtccgc actgcggatg gggcagctgg agaaccgtcg gcccttttcg
    15241 cacaagaaca gttatccgca aacgcctaag tgtttcaggg ggagagagcc aggggaaacc
    15301 ttccttcact acctcctact cagcttatgg gcctcgatgc gccattcagg tgcaggacga
    15361 ccgattcaac gcgagtccat ggattcgatg gctgacggga tactggtcga ctatggttct
    15421 ttatccatca ttaggcaccg ggatcaccag cggcggcagg tcctccggtc ggtctacaac
    15481 tccgagcacc tggccgtcag acagcgaccg gacttgcatg atggccgaat catcgatggg
    15541 tcggccaccg ggaagttgca cgcgtcgaag gatcagcgag cctctgcctg tgccaacgac
    15601 gacctgtcca tcagcgtgtg cgatctgacc aggatgcaac gcgaactcgt ctgaaacgct
    15661 tgcagcgtgc acctggattc gctctccggc cgggtccgtc gtccatgccc gcggccaagg
    15721 ccaggtcgca cgtatgtggc gctccatctc cacggcgggt cggacaaaat ccagccatcc
    15781 gtccgctttg gtcatctgcc gcgtaagcgt ggctccctca ccttggggca ccggcaatag
    15841 cgagccgtca agtaatgcgg agacgttgtc ttcaagaagc atgcccgccc ggttcgcgag
    15901 cttttgagtc acggactctg tggtgtcatc caactctatg tcgatctcag tggttgcata
    15961 tacaccgccc gtgtccaggc cgcggtccat ctgcatcaga ctcacgcccg tcacttcttc
    16021 tccctgggca atcgccgcag cgatcgggtt ggcaccccga tacttcggca ggagcgaagc
    16081 gtggagattg acgcatcccc ttgcgggcag ctcgagtatc catctgccga gaatcattcc
    16141 gaaggcggct acgataatca ggtcgggccg gatgtcgatg attcgctggc ggacctcggg
    16201 atccctcaag gtggccgtct gcaataccgg cacgcccagt cgctctgcgg ctgttttcac
    16261 cgccggagca acgtgctttc tgccgcgtcc ggcggcacga tccggctggg tgacaacgag
    16321 cgcgagatca ccactgtcgc ccagggccac ccgctccatt gccggaacag caaagttggg
    16381 cgtgcccata tagatgattc gcgacgacat atgtcacatc tcctctgcca catccgccgc
    16441 gggaagagtc tacggtacac tcggtccggg tgtatgcccc cattgtgccg cagatctccg
    16501 accggagtca tgaatgcccg tgtacgtcgc tgccctgccc tcctttcagg tcgagggtgt
    16561 cgatccttca gagcgagacc agccgctaac attcgaccag ccagtaggtg aactgccgat
    16621 cgtcgatgat gctcgatgga gcagttccgt tacggacaac tcgctcaccg ccctcctcgt
    16681 ctggatcgtt ctgcttattg tgctgcaggc cgtaacctgg cctctcgtgc gccgcgtctt
    16741 cagccgcttc cctgataagg gatgggcttt cacgcgtctg actacgatcc tcctggccgg
    16801 ctacggtgtc tggttgctct cctccatcca actcatcgcc ttccgcgcaa tatgggcggc
    16861 agcggctctt ctggtgcttg ccgctgcgag ctggctcgcc gtgcgacgaa ttactaccgg
    16921 ccgcgataat ggatcgtggt ggagaaatcc ggtcattatc gtggcggaag ctgttttctg
    16981 ggcatcgttc gcgctgttcc tgacttaccg gtttatcaac cctgactcct atcaccctat
    17041 ctggggcggt gagaagccca tggagttcgc gcatctcaac gcgattctac gaagcgctca
    17101 ttttcctccg gtcgatccct ggtatagcgg cggctacatt aactactact attacgggac
    17161 gtacctggtg gcgttcctca tcaagctcac cggtataccc gtggaaatcg ccttcaatct
    17221 cgcccaaccg acgttcccgg cactgctcgc cgcgtcctcg ttcagcctgt cggcagcgct
    17281 cggcaagcgg ttgacgcgct cggcaggtgg cgcgattacg agtggcctga tcggcgcgtt
    17341 ctttgtccag ttcgcaggga atttcatcgt cgccgagcgc ctcatcgacc gcgcgcgtaa
    17401 tggtctcggc cccgtgaatg gcttttcgta ttgggtgttc gatccgacac gcgccatccc
    17461 tgacgccgcc cgcccaatca atatcacgga atttccttat ttcggtgcgc tctatgcgga
    17521 tcttcatcct cacgtaatag ccatgccctt cacaatactc acagttgcgc ttgcatggca
    17581 gatcgcatcg cagtggagaa ttctgcccgt cgccttagcg cgacgttcgg cggctcggga
    17641 ggctcggttt gccttgctcg ctcctctcgc cttggcagtg gttggacttg gcaccctttt
    17701 catgacaaat gcctgggacc tgccgatgta tgcagccatc gtcggcgttg cagtcattat
    17761 ggcgacaagg ggtattccgc gaatcgtccc gcgcctgcat gtgtccctta ccttgctctt
    17821 tgtcctcggt gtgacgacat ggctgcttat cctgcccttt acgtcgcatt acgtcgctct
    17881 cttcggagag attgagacgg tgagggaccg cacgccgctc ctggcaatcg agtcccacct
    17941 cggcggtcaa atcctcctca tgacgatcgg catccaggcg ctcgttgcat caggattacg
    18001 atttccccgg ccggccgtta caccatatgc ggtggcttca atttacctgg tcgcgatctt
    18061 tctcttcatg caatcgggaa gcaatgaggg aagcgagatt tggaaccgtg ccggtgagct
    18121 tgggacggtg gctgtcgttg tcggcatgtg gatcatagca gcgtggtctg caacgcgggc
    18181 gcgaaccgag ttcggtatgc atcctgccgc cctcaagacc cttctagtgg aggtcgcggc
    18241 aattacagtt gccctcctgg tacttgaccg actcgtacta gcgctctacc tggggatcgg
    18301 tctttcggcg gccattctct ggttctcgct gcgccggccc ggcgagcgtt tcctcgttgc
    18361 attgatcgct ggcggttctc tccttggtgc agcccttgag attgtttacc tcgtcgacga
    18421 cctgtcgggc ggcgattggt accggatgaa cactatcttc aagttctata acgaaatctg
    18481 gaatctcttc ggcatcgcca ccggtgttct ggcagggcac gctgtatggg cggctactcg
    18541 aacgcgtaga cgcctgaact cgaccgtggg ccagcgaact tatccgaacg tctccgtctg
    18601 ggccagggcc acgacgatta tcctgattcc cgtcgtcatt gcatcccttg cctacgccgt
    18661 cgtggcaacg cctatccgcc tcgaacagtc ctggggcgaa cggaacgaac cgacgctcga
    18721 tgcgtatcag tggatggaat atgcgagcat gccgctgcag cagggaggtg cactgacgta
    18781 tcaggatgat cttgcggcta tcaactggtt caacgagaag gtcccgggca ctccagtgat
    18841 cgccgaagcg gccttcggtc cctatcgatg taacggctcc cgatactcaa ttgctaccgg
    18901 actgcccgcg gtaatcggat ggcagcgcca cgagcaacaa cagcgttatc tcgaggatct
    18961 cggtagtcgc gaggctgcgc tgagagagct ctatacgact ccgtcagcgg acgtgaagcg
    19021 ggaaattatc gagcggtatg gcatcgagta catcattgta ggtgaaactg agcgccatta
    19081 ccctacggtc aatggcaacg actgtatcga caccgggtcc cctgagggca ttgctgcgtt
    19141 ggaaagcatg gccggccgtt tcctcgaagt tgccttccag caaggaacaa caaccgttta
    19201 ccgggtgacc ggcgactgat tgccctactc gggaccagca gatggggtat actcgggccc
    19261 ggtgatggga cgcttgcgtc tccttcactc ggggtgtggc gcagcctggc agcgcgcggc
    19321 gtttgggacg ctgaggtcgg aggttcgaat cctctcaccc cgaccccaaa tcaacaagaa
    19381 cagcagaaag cggggtcccg atttgggacc ccgctttctg ttgttcttcg catcaggatt
    19441 aacgactgta atactcgacc acaaggtgct cggcgcagag aatcggaatc tcctctcgcg
    19501 gcggcacata gtcgaatcga gccgacagat tgttctcatc gaaggtcata tacggagcaa
    19561 ccggtccgcg ggcaaggagg tcaccgaaga tgacggactt cctgctcttg tcgcgaacgg
    19621 tgatcacgtc gccaggctta aggcgcatcg acggaatcga agccttcttg ccattaacca
    19681 tgaaatgtcc atgtgtcacg tactggcgag cggcgtagat cgtcggcgca aagccggcgc
    19741 gaagcaccaa tgcgtccaga cgagtctcca gcacctgcat caggttgtcg cctgtcgagc
    19801 ccggcatgcg gctcgccgtt gcataagccc gcgcaagctg cttctcgctg atgttgtact
    19861 ggaagcgcaa tcgctgcttc tcaagtagct gcaccccgta gtccgaggga cggcggcggc
    19921 ggcgctgacc atgctgcccc ggtgggaacg gccggttctt catgtacttc tgcgcctttg
    19981 gcgtcaatgc gacgcccagt gcacgcgatt tcttaacctt tgcaccagta aaattcacgc
    20041 aacgaactcc tgtgtcgtgc ttgatgcggc cgccaggtac catacctggt cgatttgccg
    20101 ctggtcattg ctcctgaggc agcaatctcg gaagagtttg tcccgacaga ctggcacatc
    20161 ttcagtcatg acggcatcag cgcattcgcg catcggttca gggaatgcca acaaggagcg
    20221 ccgacaccag tcagcgccca gccactaata atacaacata aggcagcgag gacaggaggg
    20281 tgagggtttg gacggatgtc gctactggtc cgcatccttt ggagcggtga catttggttg
    20341 ctgctcggag ggccgcagcc ggtcactcaa ctctcccggt ggcttctcga atactgacgg
    20401 ttcggccgta tcgctcctag gtgtaaaggc gcctaacgtt atcagggctt cctcacgctc
    20461 tgcctggggc cggtccccga taatcaactc cacaagttct tgagcctggc gggaatcgac
    20521 catcccatcc gcgtccagtc catattcccc ggaaaacgca ttgtgtaccc tattgacgtc
    20581 gacttcgaag gcggttgcca cctgatccgc cgagaaacgc actcccactc cctgatcttc
    20641 cccaaggggt tcatgctccc cgttgtccgg tgacatatca gcagggcggt gcatccgccg
    20701 catatcttca ttcatggcgt gattgctcct taacgatcgc atcccaaaca ccagcaggaa
    20761 gcaatgcata tgccagcatc gctactcctg ggctgctgcc agcgcataca cggttcctcg
    20821 caggactaca gcgaaattct gcacacctgc cgcagcaaaa aatcgctcga gaaggttgcc
    20881 cgacaaggag ccagcgtgcg ctccccgacg ctgcctcgtg ggcgatgacg accttgtccc
    20941 gatcgctaag aaaccaatga caaagccgga cgttggcgcc cgccacaatc actggcccct
    21001 tccgcacgtt ggcatccgaa ccacctctcc ccagtcctgc aaggaggaca aagcccgacg
    21061 cggggagttc cgcgccgggc ttaacacgat tcctgcaggc gacactacat ttcgtcaccg
    21121 agtgccttgc tgacgtccgt gggcttctca aacgtgctgt cggacacatt ccgcag
//
LOCUS       DASYWM010000078        12827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_359077, whole genome shotgun sequence.
ACCESSION   DASYWM010000078 DASYWM010000000
VERSION     DASYWM010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12827
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359077"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VGR29_03205"
     CDS             complement(<1..806)
                     /locus_tag="VGR29_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEV2072627.1"
                     /translation="MPRIVEIAVPPDQTDTLIEALESREDVLGLRLQQGTSVQPPGDI
                     ITISTTNRSMQTLVRLLDEHGVGSDSGTSMTTSEPVSLIVPSATERLAHDTNEATWEE
                     MESVTAKESNTTANALVVMGISGVLAAIGLATNALHLVVGAMLIAPGFEPIVRFSMGV
                     VSRSMGWRRGLMQTLQTYAALVLGAAITAGALRLLGTSPLGQEDTYLPSSVLVTYWTS
                     FSATTFMVSVTAGVAGAILVASNRAVLTAGVMVALALVPGATLVASGLVGG"
     gene            1022..1759
                     /locus_tag="VGR29_03210"
     CDS             1022..1759
                     /locus_tag="VGR29_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072628.1"
                     /translation="MALISSLIAVATLALVHIFAGKLKFLEGTPRSAWLSIAGGVSVA
                     YVFVHLLPELNEAQEHLAETVGETLSAVENHIYLIALLGLAIFYGLERAALNARRRER
                     STSNQDASRSWVLWLHVASFAAYNALIGYLLHERAKEGEGRTLALFAIAMGLHFVATD
                     YGLREHYRQGYEHFARWIFAGVLLLGWTVGWMGEVSETNRSVLLAFLAGGVILNVLKE
                     ELPEERESRFWAFVIGAGAYTALLLAM"
     gene            complement(1894..3987)
                     /locus_tag="VGR29_03215"
     CDS             complement(1894..3987)
                     /locus_tag="VGR29_03215"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV2072629.1"
                     /translation="MPVPHDYTERVYAGVLGKIIGVYLGRPFEGWSYDRIMAELGEIT
                     YYVHDRMGQPLIVTDDDISGTFTFLRALPDHGNDPNLTPAQIGESWLNYIIEGRTILW
                     WGGLGNSTEHTAYLRLKEGIEPPRSGSIELNTRVVAEQIGSQIFIDGWAMVAPGDPER
                     AADFARRAASVSHDGEAIYGAQVVAAMEAQAFVESDIDRLIDTGVSLIPRDSVIARMI
                     AEIRDWYAGERDWRKARELIAAKYGYDKYPGNCHMVPNHALIIHSLLYGDDDFQKSLM
                     IVNTSGWDTDCNSGNVGCILGIKKGLAAFDGDPDWRGPVADRLYLATAEGGRGISDAV
                     AESFHIINIGRALSGEQPISPKEGARFHFDLPGSVQGFQAEDDRVTVENVAGHSESGE
                     RSLAIRYGDASGAPARVSTPTFIPPEAFTMHYTLLASPTLHPGQTVRARVTTDGDNPG
                     PLRAHLFIRHYDGSDNLVPLSGPEATIPAGESHEYTWRVPETDGQPIAQLGLEADSGS
                     ASGGTLYLDYMTWDGAPDMQLGRPGGRGEMWRRAWVNGVDQFEARWPEPYRVVQNHGT
                     GLLMQGSQDWTDYRVSAPVTVHLAKSSGVAARVGGLRRYYALLLCDDGKARLVKACDG
                     ITTLAEADFPWEVRHPYELSLVVTGDRIEAAIDDEVLFDMRDQDRPLLRGGIGLICEK
                     GCLSSDAVTVRPVGR"
     gene            complement(4094..4948)
                     /locus_tag="VGR29_03220"
     CDS             complement(4094..4948)
                     /locus_tag="VGR29_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2072630.1"
                     /translation="MMRASNTKSKGTRELSLIPNYVILSLFLLFAIGPLLVLLFNSLK
                     NRAELGNNPLGIPTSITWDNYPRAWDVGNFSTTTLNSAILVAGTVAAVLLLGGMAAYS
                     LAKLDLPGGNGLMLYLLVASSLPIHLFLVPLFFFWRQLGLVNNLFGLILIYIAVNAPL
                     AIFLLRSYMVQLPRDFEDAARVDGASEWQVFTKVVVPLSWPGFLTVGLVVSLAVWNEF
                     LLATVFLTDQSKFTVVTSYYNFTTQFSRDWTLTSAAAVMMIAPILVIFLIFQRQFIEG
                     MTQGGMKG"
     gene            complement(4951..5901)
                     /locus_tag="VGR29_03225"
     CDS             complement(4951..5901)
                     /locus_tag="VGR29_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2072631.1"
                     /translation="MGKPMTMEKSISTERLHDARASWRKGAFFRKKVLPWLFVTPILL
                     INITVVLGPALSAGYYSLTDWSGIGAANFIGLDNFRRILLEDSSYKSAFLNNLIWLGL
                     FLTVPIAMALVGASVISPIRRGALFFRLALFVPYVLPSVIVANLWRTLLDPDRGIMGW
                     LTDQGLPGTDIALLGRQNTVLPTIAFIDNWHWWGFLMVLFLAGMQNISRDLYEAARLD
                     GANRWEEFRDVTIPGIRPVLVFMVLMTAIWSFLTFDYIWILTQGGPAGASEVLSVLVY
                     KNAFQAFQAGYASAIGLTMSFFVGIIISIFIVLRRRGWEI"
     gene            complement(5984..7324)
                     /locus_tag="VGR29_03230"
     CDS             complement(5984..7324)
                     /locus_tag="VGR29_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2072632.1"
                     /translation="MTIRKRLHLIILLAMALLVAIGPTGAVTQAQDKEQVSMWFNSTG
                     GAEGANCIIEQAIDTFNEQSDGVEVTATLQANSWDATRTALAGGGGPDIVTTPGPSFA
                     FELAQSGQLMALDDFAAEYGWTENFTPWALQLGVVDDQLYSIPSEVETLVLYYNKTLF
                     EEHGWEAPQTLDELMALAEEIDAAGIIPFAHSNAEWRPTNEWFVGEFLNHLAGPEKVY
                     QALTGELEWTDPEFVSAIELLTEMQQNGWFMGGLDRYYTTQTATTFAMLAEGEAAMKI
                     EGTWAVPDLNTYFGDEGGNANEWDWVPVPSADGDPIFDLGIGTTYGINKNAQNPEAAA
                     EFLSYYFSPEVQATLAVECGVVPAPVEIEPDALEGLDPRYAEIIAAMNEASEAGDYGY
                     TTWTFWPPRSDVYIYDEIERVWAGDITAEEYLQGLQSLFDEEVEAGDIPPIPER"
     gene            complement(7514..8542)
                     /locus_tag="VGR29_03235"
     CDS             complement(7514..8542)
                     /locus_tag="VGR29_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV2072633.1"
                     /translation="MKDGQISRRRPTQADVAELASVSQAMVSYVINNTPSVSIPEETR
                     TRVLDAVRTLGYVPQSTARSLRTRKTSTVAGILPDITNPFYPAFERGIQDVAEIHGYD
                     LITCNTDGIEEKELKYLRLAEQGRVDGFVAVLFHVSAVHLRSLLERGVAIVRLEPARK
                     EVGPLSLDNIYVDNAAAAQTATSYLIERGHRTIAMLGGQNGPQHRRVLGYCQALVAHG
                     LPINEALIQGDGFKEDNGYHAMRYVLGRMPRPTAVFAANDLIAMGALAALRDTGLRVP
                     DDIAVVGFDDIPAAKLVSPPLTTISQFPEQLGRRAAEMLFERLEGTVTGGGRCELMPH
                     ELIVRESA"
     gene            complement(9153..10292)
                     /gene="dgoD"
                     /locus_tag="VGR29_03240"
     CDS             complement(9153..10292)
                     /gene="dgoD"
                     /locus_tag="VGR29_03240"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEV2072634.1"
                     /translation="MKITDIKTHFVDRFLFVTVHTDEGVTGLGESGAWAFLESSAQAV
                     ETFKRYLIGQDPLKIEHHWQYLYRWTHFRGAAIMGALSAIDIALWDIAGKHYGVPCYQ
                     LLGGPTRNKARVYYHVFGQTREQLVQGVVAAKEQGFTAVGHLTPFTDAPREDPASGTH
                     ANTMAKAIETVRQYRDAAGDAVDLCIEIHRRLTPAEAIVLARGIEPYHPYFYEDPTLP
                     DNFDAMALIADRIHIPIATGERLHTIFEFEMLLARGAVQYVRPDVCLCGGITGAKKIA
                     TLAEAHYVGVVPHNPLGPVSTAACLQIAACIPNFALQEYPLGEDRPPKSEIVKNRLQR
                     DGGYLTIPDSPGIGIELAEDAAERFPYKPLDVRTRLHVDGSVVDM"
     gene            10902..12002
                     /locus_tag="VGR29_03245"
     CDS             10902..12002
                     /locus_tag="VGR29_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072635.1"
                     /translation="MNARPVAGSGPGAHARRAGHSPNDYYVPGAILLAHILIIGLLSY
                     SWFASLWAVGWAVFDLDQSNGPETGIVLSEVASHPEVMWDRTVTVSADVHDLVSDQSM
                     VIGTSSLLHFRTDTLLVLAPRPLAELADAPLDEDHTVRVTGVVRRFEPAVLEKELGIS
                     LDDPSITSHGDKVVLVAEEVEVGLPGGAVPGDPEISGSAGYEIGVTVHDVARHTDEYL
                     GETVTVSDEVEEGFLTDHAFWLGDEKLLVISAKPVSNVFVETTAYATGEVRRFDLKEV
                     EDMTGIDLEDKQFQEFQGRPFILAESVHVLADRPTQQEVKPLCERFPVGLPRSSASLQ
                     RFLSSSCSLGRLTSSPGSCSTGPSPEIMSGCY"
     gene            11993..12412
                     /locus_tag="VGR29_03250"
     CDS             11993..12412
                     /locus_tag="VGR29_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072636.1"
                     /translation="MLLIWSSICAVIAFASGGWLAARLRRIDRKGPAAAVGALVGLGT
                     LLIIIVSVQIGLGEQMDFHSVAVAVGYTEVRPATEQLPFPIVTPSPDAQTTVDENELA
                     RTRSLTTMAYLVILGLFGISAGATGSLLGVTSRHLRS"
     gene            12439..12765
                     /locus_tag="VGR29_03255"
     CDS             12439..12765
                     /locus_tag="VGR29_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072637.1"
                     /translation="MRTLADDLDGRYESAMRWNEPRPVGGPEPKPIWAKTPWIAVASA
                     DSGLVRPTGPLLTSRSTTTAENQLGTRYCQPVREAGTALFPDGAPLPERMAVHWLVDR
                     LALARN"
BASE COUNT         2499 a         3755 c         3957 g         2616 t
ORIGIN      
        1 ccccccacga ggccagaagc tacaagggtg gctccgggca cgagtgcgag agcgaccatg
       61 acgccggcgg tgagcaccgc ccggttggag gcaacgagaa tcgcccctgc cacgcctgcc
      121 gtcacactga ccataaaggt agttgccgag aacgatgtcc agtaggtcac aagcaccgaa
      181 ctgggaagat aggtgtcctc ctggcccaag ggggacgtgc caagcaagcg aagcgcccct
      241 gccgtgatcg ccgcacctaa cacaagcgca gcgtacgttt ggagtgtctg catcagaccg
      301 cgtcgccatc ccatgctccg ggagactaca cccatactga agcgcacaat cggctcgaag
      361 ccgggcgcga tcagcatggc gcccacgacg aggtgcagcg cattcgtggc gagaccgatg
      421 gcggcaagga cgccgctgat gcccatgacg acaagggcgt tcgctgtggt attgctctcc
      481 tttgcggtga ccgactccat ctcctcccag gttgcttcgt ttgtgtcgtg agcaagccgc
      541 tctgtggcgg agggaacgat gagagaaacc ggctcggagg ttgtcatgct cgttccggaa
      601 tcagaaccga cgccatgctc gtccagaagc cggacaagtg tttgcatcga tcggttcgtt
      661 gttgagatcg taatgatgtc ccccggcggc tgcacggagg tgccttgctg caatcgcagg
      721 ccaagcacgt cctcccggga ttcaagagct tcgatcaacg tgtcagtctg atctggtggt
      781 acagcgatct caacgatccg gggcatggtc cctcctgaca aagcaatgct tgctggcatc
      841 ttccggtact gctcgccagt ctgcgtcatc accggcgcat agttcgtgcc gctggggtag
      901 gagaagtccc aatacggccc tcgaattgcg tgaggcccgg tattggcgcc accgtcgcat
      961 gtgggcgttg gcatccttgc tgtccaagag gcgtaccgga caacgtagga gatcagctat
     1021 cgtggccctt atcagctcgc ttatcgccgt cgccaccctg gcgctcgtgc atatcttcgc
     1081 cggcaagctt aagttcctgg aagggacgcc ccgtagcgcc tggctctcca tcgccggcgg
     1141 tgtttctgtc gcctatgttt ttgtccatct gcttcccgaa ttgaacgagg cccaggagca
     1201 cctggcggag accgtgggtg agacattgag cgcggtggag aatcacatct acctgatagc
     1261 cctccttgga ctggccatct tctatgggct agaacgggct gctctcaatg cccgcaggcg
     1321 cgagcgctcc acgagtaacc aggacgcctc ccgatcctgg gtcctgtggc tgcacgtcgc
     1381 ttcgtttgct gcgtacaacg ccctgattgg ctacctgctc cacgagcggg caaaggaggg
     1441 agaggggcgt acccttgcgc tgttcgcgat tgcgatgggg cttcatttcg tggccaccga
     1501 ttacggttta cgcgaacact acaggcaggg atacgagcac tttgcccgct ggattttcgc
     1561 cggtgtgctc ctgctggggt ggacggttgg ctggatggga gaggtgtcag aaacgaatcg
     1621 atcggtgctg ctggcattcc tggccggtgg cgtcatcctc aacgtcctga aggaggagct
     1681 gcctgaggag cgcgagagcc gcttctgggc cttcgtgatc ggtgcgggcg cctacaccgc
     1741 cttgctactg gcgatgtgag cggaggatcg gatctattgc cgtatcgagg aggcatcttg
     1801 gtcgtgagcg ggggagagct gccagtatgc ggagaggggc cagagggata gttcctggaa
     1861 ctgcccctcc atgtgaacag gcgcccgatg gaactatcgg cctaccggac gaactgtgac
     1921 cgcatcgctt gacaggcagc ccttctcgca gatgagacca atgcccccgc ggaggagggg
     1981 tcggtcctgg tcccgcatgt caaagaggac ctcgtcatca atggctgcct caatccgatc
     2041 gcctgtgacg accagcgaga gctcgtaggg gtggcggacc tcccatggga aatctgcctc
     2101 ggcgagcgtc gtaataccgt cgcacgcttt cacgagccgt gccttgccgt cgtcgcagag
     2161 caggagcgca tagtagcgac gaagaccgcc gacccgggcc gcgacaccgg acgatttcgc
     2221 gagatggacg gtgaccggag cactgacacg gtagtcggtc cagtcctggc taccctgcat
     2281 caacaagccc gtgccgtgat tctggaccac gcgatagggc tctggccacc tggcttcaaa
     2341 ctgatcgaca ccattgaccc aggcgcgccg ccacatctcc ccccgtcccc cggggcgtcc
     2401 gagctgcatg tccggagccc cgtcccatgt catgtagtcc aggtagagtg tgccgcctga
     2461 agcactaccg gagtcggctt cgagacctag ctgggcaatc ggttgcccgt ctgtctctgg
     2521 aacgcgccag gtgtactcgt gtgattcccc ggcagggatc gtcgcctcag gaccgctgag
     2581 tgggaccagg ttgtcggagc cgtcgtagtg gcggatgaag agatgcgccc ggagcggtcc
     2641 cggattgtcc ccgtcggtgg tgacacgggc tcgaaccgtc tggcctgggt gaagcgtggg
     2701 cgatgcaagc agggtgtagt gcatcgtgaa cgcctcgggc gggatgaagg tcggcgtgga
     2761 cacccgcgcg ggagcacctg aggcgtctcc ataacggatg gccaggctgc gctcgccaga
     2821 ttcgctatgg ccggcaacat tctcaaccgt tacccgatcg tcttctgcct ggaaaccctg
     2881 cacggaccct ggcagatcaa agtggaagcg agcgccttcc tttggcgaga tgggttgttc
     2941 gccggataga gcgcgaccga tgttgatgat gtgaaaactt tctgccacgg cgtcgctgat
     3001 gccgcgcccg ccctctgccg tcgcgaggta gaggcgatcc gcaacggggc cacgccagtc
     3061 cgggtcgcca tcgaaggcgg ccaaaccctt cttgatgccg aggatgcagc cgacgttccc
     3121 cgagttgcag tcggtgtccc aaccagatgt attgacgatc atgagcgact tctggaagtc
     3181 gtcgtcacca tagaggaggc tgtggatgat gagggcgtgg ttcggcacca tatggcagtt
     3241 gcccggatat ttgtcgtacc cgtatttggc ggcgatcaac tcgcgcgcct tgcgccagtc
     3301 ccgctcaccg gcgtaccagt cacgaatctc ggcgatcatc cgggcgataa ccgaatcgcg
     3361 tggtatgagc gagacgccgg tgtcaatgag acgatcgatg tcgctctcga cgaaggcctg
     3421 agcctccatg gcggccacaa cctgtgcgcc gtatatcgcc tcgccgtcgt ggctcacgct
     3481 ggcggcccgg cgcgcgaagt cggcggcgcg ctcggggtca ccgggcgcaa ccatcgccca
     3541 accatcgatg aagatctggg aaccgatctg ctcggccacg accctagtgt tgagttcgat
     3601 cgatccactc ctgggtggct caatgccctc cttgaggcgg agatatgccg tgtgctcggt
     3661 ggagttgccc agaccacccc accagagaat ggtccgcccc tcgatgatgt agttgagcca
     3721 gctctcccca atctgcgcgg gcgtcaggtt cggatcgttg ccgtgatcgg gaagggcccg
     3781 gagaaaggtg aacgtacctg agatgtcgtc gtcggtaacg atcaggggct gacccatacg
     3841 gtcatgaacg tagtaggtga tctcaccgag ctctgccatg atccggtcat agctccatcc
     3901 ctcgaaggga cggccaaggt agacgccgat gatcttgccg agcaccccgg catagacgcg
     3961 ctcggtatag tcatgaggaa cgggcatgcg aagtccttat cgtgtctgtt aggagtccca
     4021 aaggtggcag tttagatgga tatcggtgtc tggtggagca agcatctttg ctcttcgcag
     4081 gcctgatacg ttcttacccc ttcattcctc cctgcgtcat cccctcgatg aactggcgct
     4141 ggaagatcag gaagatgacg agaatcggcg cgatcatcat caccgcggcg gcactcgtga
     4201 gagtccagtc gcggctgaac tgggtggtga agttgtagta gctggtgacg acggtgaact
     4261 tgctctggtc tgtcaggaag acggtagcca gaaggaactc gttccagacg gccaggctga
     4321 cgaccagccc aaccgtgagg aagcctggcc aggacagggg aaccaccacc ttggtgaaga
     4381 cttgccactc gctggcgcca tcgacccgag ctgcatcctc gaaatcgcgc ggtagctgga
     4441 ccatatagga gcggagcagg aagatcgcta acggcgcgtt gaccgcgata tagattagga
     4501 tcagtccgaa cagattgttc accaggccga gttgtcgcca aaagaagaag agcggcacca
     4561 ggaagaggtg gatcggtaag ctggaggcca cgagcaaata gagcatcagc ccgttgccac
     4621 cgggcaggtc aagtttggcc aagctgtagg cggccatgcc gcccaggagt agtacggcag
     4681 caacggtgcc tgcgaccaga atggcgctgt tcagcgtggt ggtggagaaa ttgccgacgt
     4741 cccacgcgcg tggatagtta tcccaggtga ttgacgtggg aatgccgagg gggttgttcc
     4801 caagctccgc gcgattcttg agcgagttga acaagaggac gagcagggga ccgatcgcga
     4861 agaggaggaa caggctgagg atgacgtagt tcggtatcag gctcagttcg cgcgttccct
     4921 tggacttggt gttcgaagcg cgcatcatga tcatatctcc caccccctgc gacgcaggac
     4981 gatgaagatc gagatgatga ttcccacgaa gaagctcatc gtcaggccga tggccgaagc
     5041 gtagcccgcc tgaaaggcct ggaacgcgtt cttgtagacc agcacggaca gcacctcgga
     5101 ggcgccggcc ggcccgccct gggtgagaat ccagatgtag tcgaaggtca ggaacgacca
     5161 gatggccgtc atcaggacca tgaagacgag caccggtcgg atgccgggaa tggtgacgtc
     5221 tcggaactcc tcccagcggt tggccccatc aagccgggcc gcttcgtaaa ggtcacgcga
     5281 gatgttctgc atgcctgcca ggaataggac catcaggaag ccccaccagt gccagttgtc
     5341 tatgaaggca attgttggca ggacggtgtt ctgacggcca aggagcgcga tatcggtgcc
     5401 cggcagcccc tggtcagtca gccagcccat gatgcctcga tcggggtcca gcaaggtgcg
     5461 ccagagattg gcgacgatca cgctcggcag gacgtagggg acgaagaggg ccaaccgaaa
     5521 gaagagggca cccctccgga tcggcgaaat tacgctggcg ccgacgagcg ccatcgcgat
     5581 cggcaccgtc aggaacaggc caagccagat caggttattg aggaacgcgc ttttgtacga
     5641 tgaatcctcg aggagaattc ggcggaagtt gtccaggccg atgaagttgg cagcaccgat
     5701 cccgctccag tccgtgagcg agtagtaccc agcacttagc gctggtccga gcacaaccgt
     5761 gatgttgatc agcaatatcg gggtgacgaa cagccaggga agcacctttt ttcggaagaa
     5821 cgcgcccttt ctccagcttg cgcgggcgtc gtgcagacgc tcggtgctaa tcgacttctc
     5881 catggtcatt ggctttccca tggtgtcggc cctcccttcc agcgggagtc gagtactcga
     5941 gacgaatggt gaaatccgcc ccgagtaccc aacgcttggc ttgttatcgc tcgggaatgg
     6001 gtgggatgtc ccccgcctcc acttcctcat cgaacaggga ttgcaggccc tgcagatact
     6061 cttcggcggt aatgtcgccg gcccataccc gctcgatctc gtcatagatg tagacgtcgg
     6121 aacgaggcgg ccagaaggtc cacgtggtgt agccgtagtc gccagcctcc gatgcctcat
     6181 tcatcgcggc aataatctcc gcgtagcgcg gatcgagccc ctcgagagcg tcgggttcga
     6241 tttcgacggg ggccggtacc acaccgcact ccaccgcgag tgtcgcttgg acctctggcg
     6301 agaagtagta ggagaggaac tctgccgcgg cttccggatt ctgagcattc ttgttgatgc
     6361 cgtaggtcgt gccgatgccg aggtcgaaga tcgggtctcc gtcggccgat ggcacgggga
     6421 cccagtccca ctcgttcgcg ttgccgccct catccccgaa gtaggtgttg aggtcgggga
     6481 ccgcccaggt tccctcgatc ttcatcgcag cttcgccctc ggcaagcatc gcgaacgtcg
     6541 tggccgtttg tgtagtgtag tagcggtcta gcccgcccat gaaccagccg ttttgctgca
     6601 tctctgtaag cagttcaatg gcggagacga actccggatc ggtccactcc agctcgccgg
     6661 tgagagcctg gtaaaccttt tccggaccgg cgaggtggtt caggaactct ccaacgaacc
     6721 attcattcgt cgggcgccac tcggcattac tgtgcgcgaa ggggatgatg ccagcggcgt
     6781 cgatctcttc ggcgagcgcc attaactcat ccagcgtctg gggcgcttcc cagccatgct
     6841 cctcgaacag cgtcttgttg taatagagaa ccagggtctc gacctcgctg ggaatgctgt
     6901 agagttggtc gtcgaccacg ccgagctgca gtgcccacgg ggtgaagttt tcagtccagc
     6961 cgtactcggc cgcgaagtca tccaacgcca ttagctgccc ggactgggcg agctcaaagg
     7021 cgaatgaggg tcccggcgtc gtgacgatgt ccggccctcc gccgcctgcc agcgcggtgc
     7081 gtgtcgcatc ccaactgttg gcctggagcg ttgccgtgac ctcgacgccg tcgctctgct
     7141 cgttgaacgt gtcgatcgcc tgctcgatta tgcagttggc gccctcggcg ccgcctgtgg
     7201 agttgaacca catcgagacc tgttccttat cctgcgcctg ggtaacggca ccggtaggtc
     7261 cgatggcgac cagcaatgcc atggcaagca gaatgatcag gtggagtcgt ttacgaatgg
     7321 tcatttatgg gttctccttg gcggtgactg gcagtcacac gttccctgct gacggcaaga
     7381 gcggtcggtg taccggcttg aacgttcact gctctccgat tgacgacagg tgcgcattac
     7441 gcctgtgctg acctcccttt ctgtttgccg ctcctgggtg aggagcatcg gtgctccacg
     7501 cgaagcgatc gcattaggcc gactcccgga ctatcaactc gtggggcatg agttcacaac
     7561 gtccgcctcc tgtaacggtt ccttccaaac gctcaaagag catctcagca gctcgccggc
     7621 ccagctgctc gggaaactgg gaaattgtgg tcaatggtgg actgacgagt ttggcagcgg
     7681 gaatatcatc gaatcccaca acggcgatat cgtccggcac gcgcaatcca gtgtcacgaa
     7741 gcgcggcaag tgcgcccatt gcaatcagat cgttcgcggc gaagactgcc gtcgggcgag
     7801 gcatgcgtcc aaggacgtaa cgcattgcgt ggtatccgtt atcttcttta aaaccatcac
     7861 cctggatcag cgcctcattg attggcaggc cgtgcgcgac aagcgcctga cagtacccaa
     7921 ggacgcgccg atgttgcggg ccgttctggc cgccgagcat cgcaatcgtg cgatggccgc
     7981 gttcaatgag ataagacgta gccgtctggg cggcggcggc attgtcgaca tagatgttgt
     8041 ccaaggaaag tgggccaacc tccttgcgtg ccggttccag gcgtacgatg gcgacaccgc
     8101 gttcgagcag cgagcgaaga tgaacggcag atacgtggaa aagcacggcg acgaaaccat
     8161 cgacacgtcc ctgttccgcc aatctgaggt acttcagctc cttctcttcg atgccatcag
     8221 tgttgcaggt gatgagatcg tacccatgaa tctctgccac atcttgaatg ccgcgttcga
     8281 aggctggata aaagggattg gtgatatcgg gcaagatgcc tgcgacagtg gaggtcttac
     8341 gggttcgcaa gctacgggcg gtgctttggg ggacatagcc gagagtgcgt accgcgtcaa
     8401 gcactcgcgt tcgtgtttct tccgggatcg agacggaggg cgtgttgttg atgacgtagg
     8461 agaccatcgc ttgcgagacg gaagcaagct cggcaacgtc ggcttgggtg ggacgcctgc
     8521 gggatatttg accgtccttc atatccaccc tggatctcca gtttcgattg acaatctgga
     8581 cgttaaatcc gccttggtac ctcctcgcat agcatcgagg agattcaaat gtacagtctg
     8641 tttattcgta taaatggtaa aagggccggc ggagtactgt caagaagcga ctaagggact
     8701 gttgccgccc gagaacgtat caaacgctgc tgacattgca aaaaggtacg cagccacgga
     8761 taagactcca acttttatcg ttaagctgag aggcccttcg cctcattccg gctgtctgaa
     8821 gtgctcgctg gcttcggaaa gatccggctt cgactccagc atccggatga catcatctag
     8881 ttctacgccc attttgtgtt tcgcagcgat gaggcgatta tctgtgtccg tggtgggttt
     8941 ccggtgtacc cgccccggtt ctaccgcgcg gtcacacctg tcctgaacac gtacgcgtag
     9001 gttggttcac gatggatcgt tctgttctaa cgcggcgaca tgaatcaaac gttgggtgac
     9061 gtctacttcg gccacgtgcg tttaccgacg tcttcgcagg cctgccggtg cctggtcctc
     9121 tcgagcagta gggtttccag ttgacgatgt cgtcacatat cgacaaccga gccatcgacg
     9181 tgcagacgtg tcctaacatc aagcggtttg tatggaaacc gctctgcggc atcctctgcc
     9241 agttcgatgc cgatgcccgg gctgtcagga atcgtcaagt agcccccgtc acgttgcagt
     9301 ctgtttttca caatctcgct ctttggtggc ctatcctcgc caagcggata ctcctgaagt
     9361 gcgaaatttg ggatacacgc ggcgatctgc aagcaggcgg cagtgctcac ggggccgagg
     9421 ggattgtggg ggacgactcc tacatagtgt gcctctgcca aggtggcgat cttcttggcc
     9481 ccagtaatac cgccgcagag gcagacgtct ggtcgtacat actgaacggc cccacgtgcc
     9541 aacagcattt cgaattcgaa aatggtatgc agccgttccc ccgtcgcaat gggaatgtga
     9601 atcctgtcag caataagtgc catagcatcg aagttgtcgg ggagtgtggg gtcctcgtag
     9661 aagtaagggt ggtagggctc gatgccgcgg gcaaggacga tcgcctcagc tggagtcaag
     9721 cgacgatgaa tttcgatgca gaggtccact gcatctccag ctgcgtcccg gtactggcgg
     9781 accgtctcaa tcgccttagc catggtattg gcgtgggtcc cggatgccgg gtcttcgcgt
     9841 ggagcatcgg tgaatggggt caggtgtcca acggcggtga atccctgttc ttttgcagcg
     9901 accacccctt gaaccaactg ctcacgagtc tgtccaaaaa cgtggtagta gactcgtgcc
     9961 ttgttccggg ttggaccgcc aagaagctga tagcacggta ctccatagtg ctttccggca
    10021 atatcccaaa gagcgatgtc tattgcggat aacgccccca taatggcagc gcccctgaaa
    10081 tgcgtccagc gatagaggta ttgccagtgg tgctcaatct tgagcgggtc ctggccgatg
    10141 aggtaacgct tgaatgtttc aaccgcctga gcagatgatt caagaaacgc ccaggccccc
    10201 gattcaccaa gtccggtgac cccctcatcg gtatggaccg tgacaaacag gaagcggtcg
    10261 acgaagtgtg tcttgatatc agtaatcttc actgttcctc ccggcatgac gacggggcac
    10321 tgaactgtcg ccccttacga tcccaacggt agcatgatca atcggctgct tcttctgtcc
    10381 ttccgggcaa catcttgtcc gcgacagatc accccgtgtt cacgcctcgt tcactgacgt
    10441 accctgcccc attcacgccg gcctcgtatc ttgagagtag acgggccgtg aggttacgct
    10501 cgggcgaccg gggcatgggg acgccagcct ttgcacgctc catggcaggg ggttaacacc
    10561 acattggaca ccatcaacgc cttgggtgtg gaacttgagg ccaggatgcg ggccgccatc
    10621 aggcaccctg agcatgacat ggagaagagg tggcagtcgg caatgccgat gacggttgcc
    10681 attctcagcc attcttaacg gtgaggtttc acacctcgcg tgtggcacca ttgaggaggt
    10741 cacacgtccg gcctgaaggg caatggcggc tcgctccatc agatgacgtt atccttcgaa
    10801 ttctgtggtg agacgggtct gtaatactac ggtccggatg gatgagccaa ggacgcagac
    10861 gtggcccaac taccagctca ggatgaggag gatacaggca catgaatgcc cgcccagttg
    10921 ccggctccgg acccggcgcg cacgcccgac gtgcgggcca ttccccgaac gattactacg
    10981 ttcctggcgc cattctcctg gcccacattc tgatcatcgg gctactcagc tactcctggt
    11041 ttgcgagcct ctgggccgtt ggctgggctg tcttcgacct cgaccagtcg aacggtcctg
    11101 aaaccgggat cgtcctgagt gaagttgcca gccacccgga ggtcatgtgg gaccggacgg
    11161 tgacggtgag tgccgatgtg cacgacctcg tgagcgacca gtccatggtc attgggacgt
    11221 cgtccctcct ccactttcgt accgatacgc tccttgtgct cgcgccccgg ccactggccg
    11281 aactggccga cgctcccctc gatgaggacc acaccgtgcg cgtcaccgga gtggtgcgtc
    11341 gtttcgaacc ggcggtgctg gagaaggaac tgggaatctc cctggacgac ccctcgatca
    11401 ccagccacgg cgacaaggtg gtgctggtgg cggaagaggt cgaggttggg ctgcccggtg
    11461 gcgccgttcc gggcgatccg gagatcagcg gcagtgctgg atatgagatt ggtgttaccg
    11521 tgcacgatgt tgcacgccac accgacgagt accttggcga gacggtgacc gttagcgacg
    11581 aggtggagga agggtttctc accgaccatg cgttctggct cggcgacgag aagctcctgg
    11641 tgatcagcgc gaagccagtg tcaaacgtgt tcgtcgagac gacggcctac gccaccggcg
    11701 aggtacggcg ttttgacctg aaagaggttg aagacatgac gggcattgac ctggaggata
    11761 agcaattcca ggagttccag ggaaggccct tcattctcgc cgaatccgtc catgttctcg
    11821 ccgatcgtcc tacccagcaa gaggtgaaac cactatgcga gcgcttcccg gttgggctgc
    11881 cacgctcatc agcatcacta caacgcttct tgtcttcatc ctgctcgttg gggcggctaa
    11941 cctcgtcgcc tggttcctgc tcgactggcc cgtcaccgga gatcatgtcc ggatgttact
    12001 gatttggtcg tccatctgtg cagtcattgc cttcgccagc ggcggttggc tggcggcacg
    12061 actgaggcgg attgatcgga agggtccggc ggcggctgta ggtgccctgg ttgggcttgg
    12121 caccttactg atcatcatcg tctccgtcca gatcgggctg ggggagcaga tggactttca
    12181 cagcgtggcg gtggccgtgg gctacaccga ggtgagaccc gctaccgagc agctgccctt
    12241 cccgatcgtg acaccatccc cggatgccca aacgacggtg gacgagaatg aactcgcgcg
    12301 tacccgatcc ctaacaacga tggcgtacct tgtcatcctt gggcttttcg ggatcagtgc
    12361 cggcgcaact ggcagtttgt tgggagttac ctctcggcac cttcgatcct gaccggcacg
    12421 cacagccatg tcaatggggt gcggacactg gcggacgacc tggacggccg ctatgaaagc
    12481 gcgatgaggt ggaacgagcc gagacccgtc ggtgggcccg agccgaaacc gatttgggcc
    12541 aagacgccct ggattgccgt agcctccgct gattccgggt tggttcggcc gactggccct
    12601 cttctgacga gtcgctccac gactactgcc gaaaatcagc tcggtacccg gtactgccag
    12661 cctgtacggg aggctggcac cgccctcttc ccggatggtg ctccgctgcc cgagcgaatg
    12721 gcagtgcact ggcttgttga ccgattagcg ctcgcccgta attaacctac ctgttcaccc
    12781 cccgttcacc gacgttccct gccctgttca cctggccccg ctagcgt
//
LOCUS       DASYWM010000079        18653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_302853, whole genome shotgun sequence.
ACCESSION   DASYWM010000079 DASYWM010000000
VERSION     DASYWM010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 18653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18653
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302853"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VGR29_03260"
     CDS             <1..199
                     /locus_tag="VGR29_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072638.1"
                     /translation="FAVGLQPLRPGPACERVRLGGPCAVPYSALALPPFDPTAEPEVT
                     AYAVLELSFELMQDAGATAAA"
     gene            complement(342..845)
                     /locus_tag="VGR29_03265"
     CDS             complement(342..845)
                     /locus_tag="VGR29_03265"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV2072639.1"
                     /translation="MDCKQDPGAIATREEHWNGTALEREVAGLSSESVVRRFLEEMWA
                     KGNTDLIDMVVHEDYAADGRVVGREFVRRNMRRFRTGFPDYSVRILTLVASGDDVVVL
                     FEHTGTHQGTFGGFEPTGKQMSFLESGFFKVADDQVISGDFVSDGLGLRIQLGVLPED
                     FWQNPQR"
     gene            complement(767..1339)
                     /locus_tag="VGR29_03270"
     CDS             complement(767..1339)
                     /locus_tag="VGR29_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072640.1"
                     /translation="MVLGRTKLEQVATATLGAATLVVGASIVTDGSLAKALNGFAGLT
                     WFASAAMFAWEGRKRGAPVTQWAGVLGLTAAVAFLIMPSDLFLATIGFGGAGLTAGLL
                     AGRDPMLRAKMVPAFYLPMHIGTAVLKAMGRDLLGMESSIRTEPPPTAAIVPVVMLVM
                     AMGGGWIASRIRERSRPAKSIGTAQPSKGR"
     gene            1449..1524
                     /locus_tag="VGR29_03275"
     tRNA            1449..1524
                     /locus_tag="VGR29_03275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Arg,seq:tcg)
     gene            1560..2747
                     /locus_tag="VGR29_03280"
     CDS             1560..2747
                     /locus_tag="VGR29_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEV2072641.1"
                     /translation="MRACILSIGSELILGQITDTNATFLAQELSAVGIDLVYVTQVGD
                     DPALLLDAMRRSLQYADLLICTGGIGPTDDDLTRETIAELVGEEPTLDAELLADLTNF
                     FRGRGQDMPQRNRKQAWVIPSSTVLPNPIGTAPGWLVDTDGKMIVTMPGVPREMYRMW
                     SEQALPRILERAENRVIDSVNIKTIGIGESAAEDLLHDLVVAADPQVATYAKDDGVHV
                     RVTAFGEDPAEARQRRDACRAEVLERLGAHVWGEDGDTLQSVVARELDARGTRLLIHE
                     MGTGGALSSLLAADPASSTVFLSDLVEPALQASEPAVGRAVRNAASLLTANQEAIGVA
                     LIYEGVLDERGVSHGQLTVAISDPAGQAPSVSTAQMRASLPEVQRRSALSAIEALRHF
                     LKR"
     gene            complement(2737..3246)
                     /gene="ybeY"
                     /locus_tag="VGR29_03285"
     CDS             complement(2737..3246)
                     /gene="ybeY"
                     /locus_tag="VGR29_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV2072642.1"
                     /translation="MPSPVELEVDITIQADVELPPVITEEAVASFARQVLIAEGERGS
                     WQFGIRFVDDETMRRAHANFMGIDLPTDIMTFPYDDDGFGLVPEGGETRPLAGGDLII
                     SVDRAADHAREVGWEISHELFFLICHGVLHILGWDDAADADRSRMLHRQSSLLGEWFD
                     SKERGAATS"
     gene            complement(3230..3676)
                     /locus_tag="VGR29_03290"
     CDS             complement(3230..3676)
                     /locus_tag="VGR29_03290"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEV2072643.1"
                     /translation="MSLRSQLETDMKAAMRARDNERRDAIRYVLSAVKNAEIDKRAEL
                     TLDEEVKLIRSQVKQRQDSIEQFRSGGREDLAAKEESQVAILEKYLPQQMSDEELWEF
                     VKQGIAETGAEGPKDMGKVMGALNPRTGGRVDGKRLSSAVRDALAG"
     gene            complement(3708..4061)
                     /locus_tag="VGR29_03295"
     CDS             complement(3708..4061)
                     /locus_tag="VGR29_03295"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEV2072644.1"
                     /translation="MSDHQEACIFCRIAAGDMGTAFVAATDHVVAFDDISPQAPVHVQ
                     VIPKRHIASVHELGSGDAALWAEILDVANRVAVEKGVDESGFRLVSNAGPDSGQEVHH
                     LHVHVLGGQKLGPIA"
     gene            4137..5033
                     /gene="mutM"
                     /locus_tag="VGR29_03300"
     CDS             4137..5033
                     /gene="mutM"
                     /locus_tag="VGR29_03300"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEV2072645.1"
                     /translation="MPELPEVETVRRIAAPLVIDRSITGITMNPTFTCVLEAPDGMDP
                     VSSLIGRTIVDVDRRGKYLLFLLDADLWMVIHLRMTGRLLVVDREAPPVRFEHLGIAL
                     DNGKDLRFGDQRKFGRVRVTLPEEVEALSSRLGPEPFDRHVTGRWLHGALGRRSGKIK
                     AVLLDQHLLAGLGNIYVDEALFRARIHPERIARDLSLEEAKRLIGAIRHVLRSAIHNQ
                     GTTFSSFENPYGEAGSNADFLRVYGKGRGALPCPRCATPLEHLVVGGRGTTFCPRCQL
                     LRGPDTPSAIPAPPSRNDGAPG"
     gene            5257..5703
                     /locus_tag="VGR29_03305"
     CDS             5257..5703
                     /locus_tag="VGR29_03305"
                     /inference="COORDINATES: protein motif:HMM:NF021516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HEV2072646.1"
                     /translation="MLQATALIGIGLMAAVDEIIFHQLLAWHHFYDRSTSDIALLSDG
                     LLHAAELVAVVAGFFMWADLRDRNIAAPRLAWGGFLAGAGGFQIFDGLVDHKVLRVHQ
                     VRYGVELFPYDLAWNATGIVLLILGVILIRQAPSRDFSRSSHDAGD"
     gene            5712..6548
                     /locus_tag="VGR29_03310"
     CDS             5712..6548
                     /locus_tag="VGR29_03310"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEV2072647.1"
                     /translation="MEQHAQEVHTNALEHSGGWLIMLLLLIPLLLYLGAVMKEHRRQR
                     PWSKLRSLSYISAIALIAVAVSPMVTSATHHDPRGHMAQHLLLGMFAPLGLVLAAPIT
                     LLFRTLPLQAGRSLARFLRSRTGHLLSHPFMALVLNIGGMYVLYLTPLFRLSLSHPAL
                     HILVSVHFLAFGYLYIWAIAGPDPAPRRPGMRVRTAVLGTGIAAHAFLAKLMYAHALP
                     AGTPFSTAEVRDAAMLMYYGGDVAEFLLAVALFASWYRGRRRSRARRRARPVLVISTI
                     RL"
     gene            6598..7896
                     /locus_tag="VGR29_03315"
     CDS             6598..7896
                     /locus_tag="VGR29_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV2072648.1"
                     /translation="MTDSAYRGERSPAPSVVLLMSPASYRARAFTAAAGSIGLDVVRA
                     VDLPDGLANHVPGDISVDFTDPDSAVERLIGFAKMQERLARPVGAILAIDDRGALIAS
                     RASGTLGIPHNDPDSALAARDKFVMRERLAAHGVPVPDYQRFPASSDPETFSAELAYP
                     CVVKPLLLSGSRGVIRANTPIELNVAFRRTRAILEASGMSASEHCILIERFVPGVEVA
                     LEGLLTSGELRTLALFDKPDPLDGPFFEETIYVTPSRLPSDVQADISRRTGEAAAALG
                     LREGPVHAELRIDLERGNIRVIELAGRSIGGLCSSILEFGSGIRLEELILRHAAGLPL
                     PSLAGNDGAAGVMMIPIPRGGMFRGVDGLEHAHQVTGVTGIEITAPLNQPIVPLPEGE
                     SYLGFIFGRSDNPAHVEAALREAHERLEFQIGPVISLNKM"
     gene            complement(7920..8228)
                     /locus_tag="VGR29_03320"
     CDS             complement(7920..8228)
                     /locus_tag="VGR29_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEV2072649.1"
                     /translation="MARTVDCAKLGRELPGLEKPPFPGELGQRIYDNISAEAYAMWQP
                     HMTIMINHYGLNPADPETRKMLRGEMEEFFFGENARMPEGWVPADAAPTKGGGVPQRK
                     "
     gene            8579..9421
                     /locus_tag="VGR29_03325"
     CDS             8579..9421
                     /locus_tag="VGR29_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEV2072650.1"
                     /translation="MASGVELSALVLPKPAQSLPRQSSSPVVALAAANRIALIQLDTR
                     SALSEPKMRATLGLIAPDLIVVACFPWRLPEWIVSLPRLGSLNIHPSLLPDGRGPEPV
                     FWAFRWGLSRTGVTFHFMDAGWDTGPLVAQREMIIPEDATVVTLEQSLARLGAHLLTE
                     HLPPALDQPLAARPQPVCAARYAPFPGADDLLVSTALTARDAARFIHAIAPRYGAIEV
                     LVLATGQRLAVNRVVSMTEEMQVPVLQVNGTDLVHIAFASGTLVCQMVTVTSPLSLHV
                     PQIR"
     gene            complement(9481..10770)
                     /gene="kynU"
                     /locus_tag="VGR29_03330"
     CDS             complement(9481..10770)
                     /gene="kynU"
                     /locus_tag="VGR29_03330"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEV2072651.1"
                     /translation="MTEPDTDTEAYARSLDANDPLARFRKRFHLLPETIYLDGNSLGL
                     LSHDAEAAVLAAMQSWKEQGINGWLEADPPWFTLGEALGGRMAALVGAGPESVVVTGT
                     TTVNLHQMVGSFYRPSGVRRKILATALDFPSDIYALQSQVRQQGGDPARDLALVPSRD
                     GRSVEEEDIIAAMTDDVALVLLPSVLYRSGQLLDIPRLTEAAHQRGALIGWDCAHSVG
                     ALPHAFDEWDVDFAVWCSYKYLNAGPGAVGALYVNPRHFGQEPALAGWWGYRKDQQFE
                     MAHHWQGASHAGAWQISTIPLLSSASVLGSLSIFEEAGIDAVRSKSLNQTDYLIRLIR
                     DSGLGNDPYRYRIGTPQQHSRRGGHLAIEHEAAARIAQALIARKVIVDFRKPNVIRLA
                     PIALYTTYHELWRAVQVLREIIETGEQLENMEVRSVT"
     gene            10965..11897
                     /locus_tag="VGR29_03335"
     CDS             10965..11897
                     /locus_tag="VGR29_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072652.1"
                     /translation="MPIYALHENPEWFPPFAAAFDAAGIEYEEWLLTSGLIDLDTAPP
                     EGIYWNRLSASSHTRAHVWSKDYTRAVLDWLEGHGRRVVNGRSVVEMEVSKVRQLTAL
                     KAFGIDVPRTVAVVGKEDLIAAAATMPVPFITKHNQGGKGLGVQRFNDLDSFRAYAAS
                     ELFEEPVDGITLLQEYVEPHDGFITRVETVGYEYIYAISADAARGGFQLCPADACEMD
                     NNGRPIEAPSLFALREGFQHPIIDVFLGFAQKHHLEIAGFEFIEAADGRLLTYDVNTN
                     TNYNPDVEAVAPRSGPAEIVKYLQRVQAEVYALC"
     gene            complement(12189..13490)
                     /locus_tag="VGR29_03340"
     CDS             complement(12189..13490)
                     /locus_tag="VGR29_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEV2072653.1"
                     /translation="MKYSSFVLPTLMVLLSFGWATAYAEESTPPANVACAIEPRSASA
                     VMELLYNPAGTPFDMPSESLQITGESDFPSGEEVDDTTIATVDDLAQQYAACVNSGDF
                     LRYSALYTDELLLKQGYQTYWPHEDVLAMLESGSEAAAEPEFVVGPSNSAWLLQDGRV
                     GAAFVFERGNAAENVFIVFEQQDGQWRLDEIFPSVAMNPVAPVSGYQVVAEYPHDPGA
                     FTQGLVLRNGELFEGTGLEGESTLRRVDLESGDVLQMRELDEEYFGEGISILNGRIYQ
                     LTWQSGVAFVYDAETFELLKTFSYDGEGWGLTTDGQRLIMSDGTDTLVFRDPETFDVT
                     GEIDVRDSGTPVANLNELEYVEGEVWANVWQTDCVARIDPGSGHVVGWVDLSGLLSEE
                     ERETYDVDVLNGIAYDSESERIFVTGKLWPKLFEIEVAPAD"
     gene            13795..14067
                     /locus_tag="VGR29_03345"
     CDS             13795..14067
                     /locus_tag="VGR29_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3854 domain-containing protein"
                     /protein_id="HEV2072654.1"
                     /translation="MLTLLRDSTVPLWITEGCKKVDSALSNGIGCIVSLTGVENWQSD
                     GMALPDWKEIALRGRRVVIAFASDVMVKASVRSALERFAAWRTAGE"
     gene            14529..15695
                     /locus_tag="VGR29_03350"
     CDS             14529..15695
                     /locus_tag="VGR29_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072655.1"
                     /translation="MWMRLLRHSVLIVIAVSVVMSACVSFPELTVARSESPSIRITKK
                     ICDNSSFPVFVESHCYQDDSASFEIETPNLQHATTLRSGNQFKPSPELFAQGDMWRII
                     DTNESNHAPVTVLSCLQYRPGEDLMLGVAPVGNLRGLQRWDVWWTAADAGNGERYAPS
                     LECIWFELPNVAEVPAVLSLQAFTSNGSYLYWTDAEHTGLGEFPRGESGEKLEANIVM
                     SNLSNGEVYSFAADAYGQVLVPADDYVLQSTDTGYEGIVSMLPGATVLTEIGLGGTAP
                     DSGTSPEIAEWGVIVQYCSANACLPVVGATVYYESLDGSTSGYCVTEATNAPAGPTGV
                     CGFEFVWGVPVRLTLDTSTLPEGTVLVSANPIDYLVQENLGGDPAVPIFELGPA"
     gene            16003..18423
                     /locus_tag="VGR29_03355"
     CDS             16003..18423
                     /locus_tag="VGR29_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2072656.1"
                     /translation="MSKKASLPEVQKGATSGAGNGEIRQLPVPLSPFLGREADIAQVL
                     CLLDDPAVRLLSLTGPGGVGKTRLAIEVAEEARHAFADGVIFVPLAAVRDPELVLPAI
                     AQAVGVRETAGDQLSALVHAAMANREMLLLLDNFEHVIPAAPVVARLLSHCPKVTVLS
                     TSRERLHLSGEHEYPVAPLQVVETSAKPSLDEIAGSDAILLFVSRAQSVKPEFTLTEN
                     NGSAIADICRRLDGLPLAIELAAARIKVLPPAALRARLEHALPILTDGSRDLPAHQQT
                     MRTTIAWSYDLLSLAERLFFQHLAVFVGGFTLKAFETVCGDLASPELDALTALTSLVD
                     KSLVRVVEVPDGTARYLLLETVREFALERLRDQGSEFDARAAHAVYFRDLAERLEAEM
                     YGPAMHRCLDRFDEEMANMRSALTFFAEAADAGSELRLAGMMAEFWDYRGFIPEGIDR
                     LSGAIARGRDAPPRPRAKALFELALLYSMVGDTARADEVSAESLPVAREDGDRYRLAM
                     ALYVRAECLRKGGSDRWDEVIELLEEAVAHAGCFDPPPPIWGPALGELGRVLTRRGER
                     QRGTALIHEAVEVQRSMGQRFGAGLQLATLARLDREAGNLTQAAVRYGESLRLFSEDG
                     VDLHIAGSVAGLARLAAARGWAEHAALLLGAVDAVAERTGATAQLAGEEDLEWIRETA
                     NGALGAERFTAAIVTGRQLPFAAAVAAALALAETLAQDADLPAAIPSPVLLPFNVPPT
                     PARFGLTPREQEVLGLLIKGKSNPEIAKQLFISPRTATTHVTNILAKFGVETRAAAVA
                     FAFQHNLV"
BASE COUNT         3559 a         5867 c         5504 g         3723 t
ORIGIN      
        1 cttcgcagtc ggtctccagc ccctacggcc cggccctgcc tgtgaacgag tgcggctcgg
       61 aggaccttgc gctgtcccct actccgcatt ggcgctgcca cctttcgacc caaccgctga
      121 gcctgaggtg accgcctacg cggtcctgga gctctccttc gaactgatgc aagacgcggg
      181 cgctacagcc gctgcatagc ccctcaaatg aagctgccgg gataactacc cggcagcttc
      241 tttctcgtca gacataggga actcggattc aatagcgggt ggtgcctctg gatagatctg
      301 gagcgacccg ctggacgcag tgaggaaata cgtgcggttg atcatctctg agggttctgc
      361 cagaagtcct ctggcaggac acccagctgg atgcgaagtc ccaatccatc cgagacaaag
      421 tcgccagaga tcacctggtc gtccgcaacc ttgaaaaacc ctgattcgag aaagctcatc
      481 tgcttccccg tcggctcgaa gccgccgaac gtcccttggt gggtcccggt gtgctcaaag
      541 aggaccacga catcatcacc ggatgcaacc agcgtcagga tacgaacgga atagtcgggg
      601 aatcccgtgc gaaaccgacg catgttgcgc cgcacgaact cacggccgac cacccggccg
      661 tcagcggcat agtcctcatg gaccaccatg tcaatgagat cagtgttccc tttcgcccac
      721 atctcctcca ggaatcgccg aacaacggat tccgaagaca ggcccgctac ctccctttcg
      781 agggctgtgc cgttccaatg ctcttcgcgg gtcgcgatcg ctcccggatc ctgcttgcaa
      841 tccatccgcc ccccatcgcc atcaccagca tcacgaccgg cacgatagcc gcagtgggcg
      901 gcggttcggt gcgaatgctc gactccatgc cgagcaaatc tcggcccatg gccttgagga
      961 ccgctgtgcc gatgtgcata ggcaggtaaa acgccggaac catcttcgcc cggagcatgg
     1021 gatcgcgccc cgcaagcagc cccgccgtta gcccagcacc gccaaagccg attgtcgcca
     1081 ggaacagatc agagggcatg atgaggaacg cgacggctgc ggtcaacccc agcactccgg
     1141 cccattgggt cacaggtgca ccgcgcttcc tgccttccca tgcaaacatc gccgcggagg
     1201 caaaccacgt cagccctgcg aacccgttca atgcctttgc cagacttcca tccgtcacga
     1261 tcgacgcccc aacgaccaac gttgcggccc caagggtcgc tgtggcgact tgctccagtt
     1321 tcgtcctacc caaaaccatt tcgctggcta ccctctcccg atccagagct acgcttttct
     1381 ctattctgcc agcaattgcc tgacagggtg cggcatcgct gctatactcc gctgcgggcg
     1441 ctgtccttgc cctcgtagct caacggatag agcgtctggc ttcggaccag aaggttgggg
     1501 gttcgagtcc ctccgagggt accaccatcc atacacatac ctcgcaccta gggaatctca
     1561 tgagagcctg cattctctcg atcggctctg agttgatcct tggccaaatc accgatacca
     1621 atgcgacgtt cctggcgcag gagctttccg ccgtaggcat cgatctggtg tacgtcacgc
     1681 aggtcgggga cgatcctgcc ctgttgctcg acgcgatgcg ccggtccctc caatatgcag
     1741 acttgctcat ctgtacgggc ggtatcggcc ccacggacga cgatcttacc cgggaaacga
     1801 tcgccgaact ggttggcgaa gaaccgacgc tcgacgccga actactcgct gacctgacca
     1861 atttctttcg cggtcgtggc caggacatgc cccagcggaa caggaagcag gcctgggtca
     1921 tcccctcctc taccgtcctt cccaacccga tcggcacggc gcctggttgg ctggtcgaca
     1981 ccgacggaaa aatgattgtg accatgcccg gcgtcccgcg cgagatgtac cggatgtgga
     2041 gcgagcaagc acttccgcgc atcctcgaga gagccgaaaa tcgcgtcatc gactccgtca
     2101 acatcaagac gatcggcatc ggtgagtcgg cggctgagga cctgcttcat gacctcgtcg
     2161 tcgcggcgga cccccaggtt gcaacttacg ccaaggacga tggcgtccac gtccgggtaa
     2221 ccgcgttcgg cgaggatccg gcggaggcac gacagcgtcg cgatgcctgt cgggccgagg
     2281 tcctggaacg actgggcgcc catgtctggg gggaggacgg ggacacactg cagagcgttg
     2341 tggcgcgaga attggacgcc cgtggcaccc gactcctcat ccatgagatg ggcacgggtg
     2401 gcgcgctctc ctcgctcctc gccgccgatc ccgcgtcatc cacggtgttc ctcagtgatc
     2461 tcgtcgagcc ggcgctccag gcttcggagc cggccgtggg gcgtgcggtc cgcaacgccg
     2521 cgagcctcct cacagcaaat caggaagcga tcggcgtagc ccttatttat gagggagtgc
     2581 tcgacgagcg gggggtgtcg catggccaac taacggtcgc tatcagcgac cccgcagggc
     2641 aagcgccgtc tgtgtcgaca gcgcagatga gggcatccct accggaagtc cagcgcaggt
     2701 ccgccctgag cgcgattgaa gccctgcggc acttcctcaa gaggtagcag cgcccctctc
     2761 cttgctgtca aaccactcgc ccagtagcga cgattgtcgg tgcagcatgc gcgaccggtc
     2821 tgcgtccgcc gcgtcatccc agccgaggat gtgcaacaca ccgtggcaga tgagaaagaa
     2881 gagctcgtgg ctgatctccc atcccacctc ccgggcatga tcagcggccc ggtcgaccga
     2941 gatgatcagg tcaccgccag cgagaggccg ggtttctcct ccctctggaa cgagaccgaa
     3001 accgtcatca tcgtacggga aggtcatgat atcggtcggt aggtcgatcc ccataaagtt
     3061 ggcatgcgct cgcctcatgg tctcatcatc cacgaacctt atgccgaact gccatgagcc
     3121 gcgctcgccc tccgcaatca gcacctggcg cgcgaacgac gctaccgcct cctctgttat
     3181 caccggcgga agctcgacat ccgcctggat cgtgatgtca acctcaagct caaccggcga
     3241 gggcatcgcg caccgcgctc gaaaggcgct tgccatcgac gcgaccgccg gtcctggggt
     3301 tcaatgcgcc catcaccttg cccatgtcct ttggcccctc agcacccgtt tcagcaatcc
     3361 cctgcttcac gaattcccat agctcctcat ccgacatctg ctgaggcaga tacttctcga
     3421 gaattgccac ctgggattct tccttcgcgg caagatcttc ccgccccccg ctccgaaact
     3481 gttcgatgga gtcttgccgc tgcttgacct gcgaccggat cagcttcacc tcctcgtcca
     3541 gcgtcagctc cgcgcgcttg tcgatctcgg cattcttgac ggcggagagc acatagcgaa
     3601 ttgcatcgcg cctctcgttg tccctggccc gcatggcggc cttcatatcc gtttccagtt
     3661 gtgatctcag tgacacgcgt cttcctcctc cgcgatgtgt ctcgcgctca ggcaatcggt
     3721 cccagcttct gcccgccaag gacatgaaca tgaaggtggt ggacttcctg tccggaatcg
     3781 ggacccgcgt tggaaacaag tcgaaacccc gattcgtcca ctcccttctc aaccgcgacc
     3841 ctgttggcca catcgaggat ctccgcccaa agcgcggcat ctccggaccc gagttcgtga
     3901 acgctggcga tgtgccgctt cgggataacc tgcacatgaa ccggggcttg gggggagatg
     3961 tcgtcgaacg ctacaacatg atcagtcgcc gccacaaacg cagtccccat gtcgcccgcg
     4021 gcaatacggc aaaagataca tgcctcctga tggtcagaca tgagatcatc ctcctgagta
     4081 caatcaacca aaaagcggtg caaggccgaa cgtaatgcac ctgaacagga atgagtatgc
     4141 cagagctacc tgaagtggaa accgtccggc gcattgccgc gccgctcgtt attgaccgtt
     4201 ccatcaccgg cattaccatg aacccgacct tcacgtgcgt cctggaggca ccggacggca
     4261 tggatccggt gtcttcgctg atcggccgga ccatcgtgga tgtcgaccgt cgcgggaaat
     4321 acctgctctt cctgctcgac gccgatctat ggatggtcat ccacctccgc atgacgggca
     4381 ggcttctggt cgtcgaccgg gaggcgcctc ccgttcgctt cgagcatctg gggattgcgc
     4441 tggataacgg gaaggacctc cgcttcgggg accagcgcaa gttcggacgt gtgcgcgtca
     4501 cactcccgga ggaggtcgag gcgctctctt cccggctcgg ccctgagccc ttcgatcggc
     4561 acgtgaccgg ccgatggctg catggggcac tcgggcgccg gtcgggcaag atcaaggcgg
     4621 tgctgctgga tcagcacctg cttgccggac tggggaacat ctacgtcgac gaggcactct
     4681 tccgcgccag aatccatccg gagagaatcg cccgagacct ctccctcgaa gaggcaaaac
     4741 gattgattgg tgcaatccgg catgttctcc ggtcggcgat ccataatcag gggacgacct
     4801 tttcctcctt cgaaaacccc tatggggaag ctggttcgaa tgcagatttc cttcgcgtct
     4861 atggaaaggg ccggggagcg cttccctgcc cccgctgtgc cacgccgctg gaacatctcg
     4921 tcgtcggagg ccgtggcacg acgttctgcc cgcgctgcca gcttttgcgg ggaccggaca
     4981 ccccatccgc aatcccagcc ccgccctcga gaaatgacgg agctcctggc tgacaaccca
     5041 aaagcccttc ggcccttcct gtcgccgcta atgtatctgc tcaaactgca cggctggagg
     5101 caatccgggg aaagcttctt gccaatatgt cctccagtag ccaagggacg agaaacagga
     5161 ggaacgatcc tggtggcgcc tgcaatgccg atcatagtgt gtcgaggaga aggggctgcg
     5221 gtgagaagta gcgagacgag gcaggagcga atccgcttgt tgcaagcaac agccctgatc
     5281 ggtattggtc tgatggctgc ggtggatgaa atcatcttcc atcagctgct ggcctggcac
     5341 cacttctatg atcggtccac gtccgatatc gccttgctct ccgatggtct cctgcacgcc
     5401 gccgagctgg tcgcagtcgt tgccggcttt ttcatgtggg cggatctccg ggacaggaat
     5461 atagctgcgc cacgcctcgc gtggggaggg tttctcgccg gcgcgggagg cttccagatc
     5521 ttcgatggtc tggtggatca taaggtcctt cgggtgcacc aggttcgata tggcgtagag
     5581 ctctttcctt atgaccttgc ctggaatgcc acaggcatcg tcctgctaat cctgggcgtc
     5641 atcctgatca ggcaagcgcc ttccagagac ttctcgcgct cctcgcatga cgcgggggat
     5701 taggacagag catggagcaa cacgcacagg aggttcacac taacgccctg gagcactccg
     5761 gcggctggtt gatcatgttg ctccttctta tacccctgct cctctatctc ggcgcggtga
     5821 tgaaagagca tcgacgacaa cggccatgga gcaagttgcg cagcctcagc tacatttcgg
     5881 cgatcgccct gatagcagtc gccgtctcgc cgatggtgac aagcgccact caccacgatc
     5941 cacgcgggca catggctcaa cacctgttgc ttggaatgtt tgcaccactt ggcctcgtcc
     6001 tcgccgcccc gataaccctt ctttttcgca ctctcccact ccaggcaggc cgatccttag
     6061 cgcgattcct gcgcagccgg acaggtcacc tgctttcgca cccgttcatg gctttggtac
     6121 tgaatatcgg cgggatgtac gtcttatatc tcacgccgct cttccggctg agcctctctc
     6181 atccagcgtt gcacattctg gtcagcgtgc acttcttggc attcggttat ctatacatct
     6241 gggcgatcgc ggggcctgac cctgctcccc gacggcccgg catgcgcgtc cgcacagcag
     6301 tccttggcac agggatagcc gcccacgcat ttcttgccaa gctaatgtat gcccacgcac
     6361 tccccgcagg aacacctttc tccaccgcag aggtacgcga cgctgcaatg ctcatgtact
     6421 acgggggaga cgtggcggaa ttcctccttg ccgtggcact gtttgcctca tggtaccggg
     6481 gccgtcgtcg gagtcgggca cgtcggcggg caagaccggt tcttgtaatc tccaccatcc
     6541 gactgtgatg gatctggggt tcgtcattcc caaaggacgt agtacggagg gtgcttgatg
     6601 accgattccg catatcgggg tgaaagatcg cctgctccat ccgttgtcct gctcatgagt
     6661 ccggcatcct accgcgcccg ggcgtttacg gcggcggccg gttcgatcgg actcgatgtc
     6721 gtgagagccg tcgatctacc ggacggattg gccaatcacg tgccggggga tatatccgtg
     6781 gattttacgg atcctgactc tgccgtcgaa cggctcattg gcttcgccaa gatgcaggaa
     6841 cggctcgcga ggccggttgg tgccattctc gccattgatg accgaggtgc gctcatcgcg
     6901 tcgcgagcga gtggcacctt gggaatccca cacaacgacc ctgattctgc tctggcggcc
     6961 agggacaagt tcgtgatgcg ggagcggctg gccgcgcatg gcgttcctgt gcccgattac
     7021 cagcgctttc ctgcgtcgtc cgacccggaa accttttcag cagagctcgc ttacccctgt
     7081 gttgtgaagc cgctgctgct ctccggtagc cgaggagtca tccgggccaa cacacccatt
     7141 gagctcaatg tcgccttccg acgtacccgt gccatcctgg aagcgtctgg aatgtcggcg
     7201 agcgagcact gcatcctgat agagcgattc gttcccggcg tcgaggtcgc actcgaaggc
     7261 ttgcttacgt caggagaact tcgaacgcta gcgctcttcg acaaaccgga tccgctcgat
     7321 ggacccttct tcgaggagac gatctacgta acgccatccc gattaccgtc ggacgtgcag
     7381 gccgacatct cccggcgaac gggagaagca gctgccgcgc ttggattacg agagggccct
     7441 gtgcatgcag aactgcgaat cgacctcgaa cggggaaata tccgggttat cgaacttgct
     7501 ggacgttcaa tcggtggtct ctgctccagc attctggagt tcggctccgg aatacggctg
     7561 gaagagctga tcttgcggca tgctgcgggg ctgccactgc cctccctggc ggggaacgac
     7621 ggtgcagcag gcgtcatgat gatcccgatt cccaggggcg ggatgttccg tggcgtggac
     7681 ggcctggagc acgcgcatca ggtaactgga gtgaccggga tagagataac ggcgccgctc
     7741 aatcagccaa tcgtgccgct ccccgagggg gaaagctatc tcgggtttat ctttggccgc
     7801 tccgataatc cggcgcatgt cgaagcggcg ctgcgtgagg cgcacgagcg tcttgagttc
     7861 caaatagggc cggtgatctc gctgaacaag atgtgaggta cctgcgatgc agccctcccc
     7921 tacttccgtt ggggaacacc gccgcccttg gtaggcgccg catcggctgg tacccagcct
     7981 tcgggcatcc ttgcattctc cccgaaaaag aattcttcca tttcaccgcg aagcatcttg
     8041 cgggtttcag gatcggccgg gttgaggcca tagtgattga tcatgatcgt catgtggggc
     8101 tgccacatgg catacgcttc ggctgagata ttgtcgtaga ttcgctggcc cagctctccg
     8161 gggaacggcg gcttctcaag gccggggagc tctcgcccca gttttgcaca atcaactgtt
     8221 cgggccattg tctgtctcct tcataatgag gctgcagggt ggtatcaccc gtccccaagc
     8281 ctctgccacc ggatcgccaa gggcatcaaa gtggcgagaa tggccatgcg agtgtaccac
     8341 cggggaggct cgtggcctac gcggggagtg tcaggagttc ggacccgccg atcgatcctt
     8401 tccccggtta gcgccgtttc ctcgggaatt ggcaagggag caaaactggc gaatgtggga
     8461 gcatggcggg cctacggatc cgcacataga caggggaata agccgcccta gtccgaggcg
     8521 ggttgtcctg ttcggtatgg attgcctgtt caccctgccc attctcgagg cgctagccat
     8581 ggcgtccggc gtcgagctgt cagcactggt tctcccgaag ccggcgcaat ctttaccgcg
     8641 ccaatcttcc tctcctgtcg tggcattggc cgccgcaaac cgcatcgcac tcatccaact
     8701 tgacactcga tcagcgttga gcgagccaaa gatgcgagcg acgcttggtc tcattgcacc
     8761 ggatctcatt gtggttgcct gcttcccctg gcggttaccg gagtggatag tgtcattgcc
     8821 acggctcgga agcctcaaca tccacccatc attactcccc gacgggcgcg gccctgagcc
     8881 ggtgttctgg gcgtttcgct gggggctttc cagaacggga gtgacctttc acttcatgga
     8941 cgccgggtgg gacaccggac cactggttgc acaacgggag atgattatcc ccgaggatgc
     9001 cacggtcgtg accctggagc aatcgctggc ccggctcggg gcacacctgc tcacggagca
     9061 tttgccaccg gctctcgatc agccgctggc cgcacgccct caacccgtat gcgccgcacg
     9121 gtatgcgccg ttccccggcg cggacgacct gttggtatcc actgccttga cggcacgcga
     9181 cgcggcacgc ttcatccatg ctattgcgcc tcgctacggc gccatcgagg tcctcgtgct
     9241 cgctaccggg caaagactgg cggtgaacag ggtcgtttct atgacggagg aaatgcaggt
     9301 tccggtactg caagtaaatg ggaccgacct cgtccacatc gccttcgcaa gcggaactct
     9361 cgtttgccag atggtcaccg tgaccagtcc actctcactc catgtcccac agatccggta
     9421 gagcgcctgg aggaaccaaa agccggaaaa tgcctgcctt cgggctcatg cgtggaacga
     9481 tcaggtgacc gacctcacct ccatgttttc aagctgctcg cctgtctcaa tgatctcgcg
     9541 cagcacctgc acggcccgcc aaagctcgtg gtaggtcgtg taaagggcaa tcggcgcgag
     9601 gcggatcacg tttggcttgc gaaaatcgac gatcaccttg cgagcgataa gcgcctgcgc
     9661 gatccgcgcg gccgcctcat gctcgatcgc cagatgtccg cctcgcctcg aatgctgctg
     9721 cggagtcccg atacggtatc ggtatgggtc attccctaac ccggagtccc tgataagccg
     9781 aatcaggtag tccgtctggt tcagcgactt cgagcggacg gcgtcgattc ccgcctcttc
     9841 gaatatcgac agcgaaccga gaaccgaggc actggacagc agcggtatcg tgctgatctg
     9901 ccatgcaccg gcgtgcgatg ctccctgcca gtgatgcgcc atttcgaact gttggtcctt
     9961 gcggtatccc caccaccccg ccagagctgg ctcctggccg aaatgacgcg ggttcacgta
    10021 gagcgccccg accgcgcccg gtcccgcatt gaggtatttg taggagcacc agacggcgaa
    10081 atctacatcc cattcatcga acgcatgtgg tagcgctccg actgaatggg cgcaatccca
    10141 tccgatcagc gctccccgct gatgcgccgc ttcggtcagg cggggaatgt ccaacagctg
    10201 gcctgagcga tagaggaccg acggcagcaa cacaagtgcg acgtcgtcgg tcatcgcggc
    10261 gatgatgtcc tcctcttcga cggaccggcc atcgcggcta ggaactagcg caagatcgcg
    10321 cgctggatct ccaccttgct ggcgaacctg gctttggagc gcatagatat cggagggaaa
    10381 gtcgagcgcg gtggcaagga tttttcgccg cactccagat gggcggtaaa acgagccgac
    10441 catctggtgc aggttgaccg tcgtcgtgcc ggtgacgacc acggactccg gccctgcgcc
    10501 aaccagggcg gccatccgcc cgccgagcgc ctcgccgagc gtgaaccatg gcggatccgc
    10561 ttccaaccac ccgttgatgc cctgctcctt ccacgactgc atggcggcga gcacggcagc
    10621 ctcagcgtca tgcgagagaa ggccgagcga attgccgtcg aggtagatcg tctctgggag
    10681 caaatggaac cgcttccgga accgtgccag gggatcattg gcatcaagtg accgggcata
    10741 agcctccgta tcggtgtcgg gttcagtcat cgggattctc cgcattcgtc gaatcgtctg
    10801 ttctccggct catcatgcac caaacgggcc agtcagagcg acataggagc ccgcacagcc
    10861 cggaagcagc gtaaacctgg ggcttcaact gcgaagcttg tctttccgaa gctggtatca
    10921 tttaggaagt gcagtcgccg ttgagagaga gaaagcaggc ccgtatgccc atctacgcct
    10981 tacacgagaa tcccgagtgg ttcccgccct tcgctgcggc gttcgacgca gcgggtatcg
    11041 agtatgaaga gtggctgttg accagcggct taatcgatct tgataccgcc cctcccgagg
    11101 gcatctattg gaaccggctc agtgcctcgt cgcatacccg ggcgcatgtc tggtccaagg
    11161 attacacccg agcggtcctc gactggctgg aaggccacgg tcgcagagtt gtcaatggcc
    11221 gctcggtggt cgagatggaa gtcagcaagg tgcgtcagct cacggctctc aaggccttcg
    11281 gcatcgacgt gccgcgcacc gtggctgtcg tcggcaaaga ggacctcatc gccgcggcag
    11341 caacgatgcc ggtcccgttc atcaccaagc acaaccaagg cggcaagggc cttggcgtcc
    11401 agcgcttcaa tgacctggat tcgtttaggg cgtatgcggc atcggaactt ttcgaggagc
    11461 ccgtagacgg gatcacactc ctgcaggaat acgtggaacc tcacgacggg ttcatcaccc
    11521 gtgtcgagac ggttggatac gagtacatct acgccatcag cgcagatgcc gcccgaggag
    11581 gattccagct ctgcccggcc gacgcttgtg agatggataa caatggacgg cccatcgagg
    11641 caccgagcct ttttgccctg cgcgagggat tccagcatcc catcatcgat gtttttctcg
    11701 gcttcgcaca gaagcaccat ctggagattg ccggatttga gttcatcgaa gcggccgacg
    11761 gtcgcctgct cacgtatgac gtcaacacca acacgaacta caaccccgat gtagaggcgg
    11821 tggcgccccg atcaggacca gcagagatcg tcaagtatct ccaacgggtg caggcggagg
    11881 tgtatgcact ttgctgagcg gaatacgatg tccgcgggga tttgcgcgtg tagtctgcga
    11941 cggcgaggaa atagcagtaa tgcgtatgga gcgcatgaag cctgcgcttc gtaagtgacg
    12001 ttgtgaacac catgccgtcc tgctactcat tgttaaggat cttcgatcgg gcacccaagc
    12061 ggacgcgctg tcgcccatgg ttttgccggt cgccgaggcg ctccggtgca gcgcgacccc
    12121 gtcacgacgg gtgcttcgcg atgtggtccc tatcacggca gctgaagacc ggtccaaacg
    12181 aggagcgatt agtcagcagg agccacctcg atctcgaata gcttcggcca gagcttaccc
    12241 gtcacgaata ttcgctccga ttcagagtcg tacgcgatgc cgttgagcac atctacgtcg
    12301 tacgtctccc gctcctcctc tgagagcagc ccggacagat cgacccaccc caccacatgc
    12361 ccgctcccag ggtcgatccg cgcaacgcag tccgtttgcc aaacgttcgc ccagacctcc
    12421 ccctccacat actccagctc gttcaaattg gcaaccgggg tacctgaatc acggacgtcg
    12481 atctcacccg tgacgtcgaa ggtttccggg tcgcggaaga ccagcgtatc ggtcccatcg
    12541 ctcatgatca ggcgttggcc gtcagtcgtg agtccccaac cctcgccgtc gtaggaaaag
    12601 gttttcagca actcgaacgt ctcggcgtcg tacacaaacg ccactccgga ttgccaggtg
    12661 agttgataga tgcggccgtt gaggatcgag atcccctccc cgaagtactc ttcatcgagc
    12721 tcacgcatct gcaacacgtc gccggattcc aggtccaccc tccgaagtgt cgattccccc
    12781 tccaggccgg ttccctcgaa aagctcgcca ttcctcagga ccaacccctg ggtgaacgca
    12841 ccaggatcat gggggtactc cgcgaccacc tggtaccccg acacgggtgc caccgggttc
    12901 atcgccacgc tgggaaaaat ctcgtcgagc cgccactgtc cgtcctgttg ctcgaagacg
    12961 atgaagacgt tctccgcggc atttccacgt tcgaagacga aggccgcacc aacacggccg
    13021 tcttgaagaa gccaggcgct atttgacggg ccgacaacga actccggctc tgccgccgct
    13081 tccgatcccg actccagcat ggcgagaaca tcctcatgcg gccaataggt ctgatatccc
    13141 tgcttcagta gcagctcgtc cgtatagagc gccgaatacc ggagaaaatc gccgctgttg
    13201 acgcaagcgg cgtattgctg agcaaggtcg tccaccgtcg cgatcgtggt gtcgtccact
    13261 tcttcgccgg acggaaaatc cgactccccg gttatctgaa gggattccga cggcatgtcg
    13321 aacggcgttc cggcgggatt gtagagcagc tccatcacag cggatgccga cctcggctcg
    13381 attgcgcagg caacattggc cggaggagta ctctcctcgg cataagcagt cgcccatccg
    13441 aacgagagca ggaccatgag cgtcggcagc acaaacgacg aatacttcac gggtgtttac
    13501 tccatggtga cgtgatgcga tggacaaccc gttccgaggt tcaggtgatg caactgtaaa
    13561 gcaacccggc gtcagacccg ctcgtctcct ggaacctgtt caccggtgag gtgcgggatg
    13621 agttccgtgc tgcgctcctg gcggaggtcg agcgtttgcg aagacactca tccgcctgcg
    13681 ctactacagg gagaacaatc gggcactcgc aaccaatgcg cgtcaaatca ccgaggacgt
    13741 actgcttggc tgctgccccc tcggaaagcc ccctcgccag tagtacttca ccgcgtgctc
    13801 accctgcttc gcgactcgac tgtgccgctc tggattaccg aaggatgcaa gaaggtcgac
    13861 agtgccttga gcaacgggat cggctgcatc gtcagcctga ccggtgtgga gaattggcaa
    13921 agtgacggca tggcgctccc tgactggaag gaaatcgccc tgcgtggccg tcgtgttgtt
    13981 atcgccttcg cctctgacgt catggtgaag gcatcggtgc gcagcgccct ggagcgtttt
    14041 gcggcatggc ggacggccgg tgagtaactg acgaagttaa gcgggcttga ggtgtcagct
    14101 ggattctcct ctgagttcat cggaatcgtc acgttccgac gggcaggggc aacgctttgc
    14161 taaggaaaat gtattgttgc tctgaaaaga gtgacgccat ccacatccaa ccacatgggg
    14221 ccggttagct gagggttcat gtgctcggac catggactta taccttcacc caaatgctat
    14281 gcatggatgg ttccgcctct aactcactcc agcagagcgc tcgtgtcaac cgcttttgga
    14341 tcgatcccag ctgtcctacg tcggctgtgc ccttttgccg cactcactca aacgatagct
    14401 gggggtccga ttctcgtaag caattttagt taccgggcga aatgaggggg ctgtttttgg
    14461 tggcgttatc gccaagctat agttcggtta ccgttgcaag acttggcgaa aagaaagagg
    14521 aaaagggaat gtggatgagg ctcctacgac atagcgtgct tatcgtgatc gccgtgagtg
    14581 tggtgatgtc agcgtgcgta tctttcccgg aactaacagt tgcccgttct gagtcaccat
    14641 ccatacgtat caccaagaaa atctgcgaca actcctcatt tcccgtgttt gtagaaagcc
    14701 actgctacca ggacgacagt gccagcttcg agatagaaac accgaatctg cagcacgcca
    14761 caacgcttcg gtcggggaat cagttcaagc ccagtccaga actctttgct caaggcgaca
    14821 tgtggcggat tatcgatacg aacgagtcga atcatgctcc agtcactgtc ttgtcctgcc
    14881 tgcagtaccg acctggtgaa gacctaatgc tcggtgtcgc gccggtcgga aatctccgtg
    14941 gtcttcagcg ctgggatgtg tggtggacgg ccgcagacgc cggaaatggc gagcgttacg
    15001 cgccatccct tgaatgcatt tggtttgaac ttcccaacgt tgccgaggta cctgccgttc
    15061 tcagtctgca ggcgttcacc agcaatgggt cctacttgta ctggaccgac gcagaacata
    15121 ccggtcttgg ggaattccca cgtggcgaaa gtggagaaaa actggaagca aacatagtga
    15181 tgtcgaacct gtccaatggt gaggtctact cctttgccgc agacgcgtac ggtcaggtcc
    15241 ttgttccggc tgacgactat gtgttgcaga gcaccgacac ggggtacgag ggcatcgtat
    15301 cgatgctgcc cggcgcgaca gttctgacgg agattggact aggtggaact gcccccgact
    15361 ccggcaccag ccctgaaatc gccgagtggg gagtgattgt tcagtattgc agcgcaaatg
    15421 cgtgcctgcc agtcgttggt gccaccgtct attacgaatc tctcgatggg tccacgtctg
    15481 gttattgcgt cacggaggcg acgaatgcac cggccggtcc taccggtgta tgtgggttcg
    15541 agtttgtgtg gggagtcccc gtacgtctca cactggatac atcaacactc ccagagggaa
    15601 ccgttctcgt ctccgcaaat cccatcgatt atcttgttca ggaaaacctt ggcggtgatc
    15661 ccgccgttcc catatttgaa cttggtccgg catagcaaga gtgggccatg gaagcaatcg
    15721 cgttgtcctt tgaccgttcg gagcctgatg gagtggctaa gtcgttacct tgtcagtgca
    15781 gcgacttctc aagctgaggc ccatagggtg ctgttttggt acgtgctatc tcctatcccg
    15841 tcaagcggag tcggggaaag gagataaccg ctctcgaagc cggccccccg tcccctagtt
    15901 ctcctactgt gccagctcaa tgtcgacatt tgacgtaaac ccgaacccgc cgcacaatgc
    15961 acgcaccaaa gccgggagag acgcagctac aggatggccg tcatgtccaa gaaagcttcc
    16021 ctgccagagg ttcagaaggg ggctacgtcg ggcgctggca acggcgagat acggcaattg
    16081 cccgtgccgc tgtcgccgtt cctgggccgc gaggccgaca tcgcgcaggt gctctgcctg
    16141 ctcgacgatc ccgccgtccg gttgctgtcg ctcaccggac ccggcggggt gggcaagacg
    16201 cgcctggcga tcgaggtggc tgaggaggca cggcacgcct tcgccgacgg ggtgatcttc
    16261 gtcccgctcg ccgccgtccg agaccccgag ctcgtgctac ccgccatcgc ccaggcggtc
    16321 ggagtccgtg agacagccgg cgatcagctc agtgcactgg tgcatgccgc catggcgaac
    16381 cgcgagatgt tgctgctgct cgacaacttc gagcacgtca tccccgccgc ccccgtggtg
    16441 gccaggttgc tgtcgcattg cccgaaggtg accgtcctgt cgaccagccg cgagcggctg
    16501 cacctctccg gcgagcacga gtaccctgtt gccccactgc aggtcgtaga gacatcggcc
    16561 aagccgtccc tcgacgaaat tgccggctcc gacgcgattc tcctgttcgt ctctcgtgcg
    16621 cagagcgtca agccggagtt caccctcacg gaaaacaacg ggtcggcgat cgctgacatc
    16681 tgccggcgtc tggatgggct gccgctggca atcgagctgg cagccgcacg gatcaaggta
    16741 ctgccccccg ctgccctgcg agcgcgactg gagcatgcgc tgcccatttt gaccgacggt
    16801 agtcgagacc tgccggcgca tcagcagacg atgcgcacga ccattgcctg gagctacgat
    16861 ctactctccc tcgcagagcg gctgttcttc cagcacctgg cggttttcgt cggtggcttt
    16921 accctgaagg cgtttgagac cgtatgcggc gatctcgcca gcccggagct ggacgcgctc
    16981 accgcactca cctcgctggt ggacaagagc ctcgttcggg tggtcgaggt cccggacggc
    17041 accgcgcggt atctgctgct ggagacggtg cgcgagttcg cgctcgaacg gctgcgcgac
    17101 caagggagcg aattcgatgc gcgcgctgcc cacgccgtgt atttccggga tctcgcggag
    17161 cggcttgagg cggagatgta cggaccggcg atgcaccgct gtctggaccg attcgacgag
    17221 gaaatggcca acatgcggtc cgcgctgacg tttttcgccg aggcggcgga cgcaggcagc
    17281 gaattgcggt tagccgggat gatggccgag ttttgggact accggggctt tatccccgaa
    17341 gggatcgatc gcttgtccgg tgcgatcgcc cgcggacgcg acgccccgcc ccgtccgcgg
    17401 gccaaggcgc tcttcgaatt ggccctgctg tacagcatgg tcggagacac ggcacgcgcg
    17461 gacgaggtga gcgccgagag tctgcccgtt gcccgcgagg acggggatcg ctatcggctg
    17521 gcaatggcac tgtacgtacg ggcggagtgt ctgcgcaaag gtggcagtga ccggtgggat
    17581 gaggtgatcg aactccttga ggaagccgtc gcgcatgctg ggtgcttcga tccgccgccg
    17641 ccaatctggg gacctgcctt gggcgagctt gggagggtgt tgacacgacg aggtgagcgt
    17701 cagcgcggga cggctcttat tcacgaggcg gtggaggtgc aacgttcgat gggacagcgc
    17761 ttcggtgccg gtctgcaact ggcgacgctg gcacgtctgg accgggaagc gggcaacctg
    17821 acacaagcgg ccgtgcgcta cggcgagagc ttgcggctct tctcagaaga cggcgttgac
    17881 ctgcacatcg caggttcggt tgcgggactg gcccggctgg cggcggctcg cggctgggct
    17941 gagcacgccg cgctgctgtt gggcgcggtc gatgcagtcg cggagcgcac cggcgccacc
    18001 gcccagttgg caggggagga agatctggaa tggattcggg agacagcgaa cggtgccctc
    18061 ggggccgagc gcttcacggc ggcgatcgtg acagggcggc agttgccgtt cgccgccgcg
    18121 gtcgcggcag cacttgcgct cgctgaaacc cttgctcagg atgcggatct gcctgctgcc
    18181 attccatctc ccgttctcct tcccttcaat gtcccgccga ctccagcacg tttcgggttg
    18241 acgccgcgcg agcaggaggt gctgggcctg cttatcaagg gcaaaagcaa cccagagatc
    18301 gccaaacagc tgttcatcag cccgcgcacg gccaccaccc acgtcaccaa tattctcgcc
    18361 aaatttgggg tcgagactcg tgccgccgcc gtcgccttcg ccttccagca caacctcgtc
    18421 tgaccatccc ctcgtctacg tactcgtctg atctacgtag atcaggcagg gtcacgacac
    18481 ctccgcaaat cgtacggctg acggatgttc gcagcgcacc agacgcgcat gatggaacca
    18541 cgcccaatcg agagagggcg gcgacctcat cgccggtgtg gtcacaccgt gagggttcat
    18601 cagaggaggc aggcgccatg gacaccaact tgaccgctca ctatctggct atc
//
LOCUS       DASYWM010000080         4897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_359250, whole genome shotgun sequence.
ACCESSION   DASYWM010000080 DASYWM010000000
VERSION     DASYWM010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359250"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VGR29_03360"
     CDS             complement(<1..931)
                     /locus_tag="VGR29_03360"
                     /inference="COORDINATES: protein motif:HMM:NF004792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2072657.1"
                     /translation="MPRKLQEAVVVVTGASSGIGRATAHEFANKGSSLVLAARSESEL
                     QEVVAECEQRGAKAVAVPTDVTDEAAVLALSQRAIDAFGRIDVWVNNAAVSFFGKLHE
                     VPMEDFRRVFETNVFGYVHGARAVIPVFREQGSGVLINVSSLVARMPQPYTSAYTMSK
                     HAVRALGMSLRQELVLDKQKNVHVSTVLPATIDTPFFQHAANYTGRTPKAMPPVYPPE
                     NVARMIVEMAQHPKREVYVGNIGRLMNMQMKFMPAMTERASATMVDREHLADTPSPST
                     TGNLYAPMPALNDVRGGWKDGSPKGTRAAKVGMA"
     gene            1203..1514
                     /locus_tag="VGR29_03365"
     CDS             1203..1514
                     /locus_tag="VGR29_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072658.1"
                     /translation="MDASEMRELLHGYLSEMDWAGDTTKNDILEHLAGRDEALRTMVN
                     EYVALGSYQGPDAVLDLLPAQAWEDVQGDEWRGAESQYVEDVDSNYQEGPLGQNQGNA
                     G"
     gene            complement(1762..2484)
                     /locus_tag="VGR29_03370"
     CDS             complement(1762..2484)
                     /locus_tag="VGR29_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072659.1"
                     /translation="MRRNRRGSNSGEGRLLARPGTTTERIAPGMHPLGLGDDRDAALY
                     IPEGYQPEQPAPFVLSLHGAGGNELSGLYPLQVLADEFGFILLSPASRGRTWDVILGR
                     FGPDIAFLDRALAATFERCAVDPARIAVSGFSDGASYGLSIGITNGDLFPNILAFSPG
                     FAVPGPQQGEPRIFVSHGIRDEVLRIDYTSRRVVPVLQDAGYEVRYREFDGPHTVPGK
                     IAREAVEWFLAGEERASETPAP"
     gene            2815..3813
                     /gene="mgrA"
                     /locus_tag="VGR29_03375"
     CDS             2815..3813
                     /gene="mgrA"
                     /locus_tag="VGR29_03375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEV2072660.1"
                     /translation="MTYTPDDQRYDAMRYRRTGRSGLLLPEISLGLWQNFGDTRPLET
                     QRSILRRAFDLGVTHFDLANNYGPPYGAAEENFGRIFAQDFRPWRDEMIISTKAGWDM
                     WPGPYGDHGSRKYLLTSLDQSLKRMGLEYVDIFYHHRPDPETPIEETASALATAVQSG
                     KALYAGVSSYDADQVGAMVEALAEHKIRLLIHQPSYNMFNRQIEDRLLPLLKEEGIGP
                     IVFVPLAQGLLTDRYLKGDIPADSRAATSPTLSGGMITEAYLERARALNEIARNRGQT
                     LAQMALVWILRKPHITSALIGASSVGQLEENIAALNSAPLSEDELAAIEPHATPLT"
     gene            3901..4119
                     /locus_tag="VGR29_03380"
     CDS             3901..4119
                     /locus_tag="VGR29_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072661.1"
                     /translation="MTDDVQREIEANERRARDQQDVGIEEDALRGNDDSTFLDDALDG
                     IPGIGDDESDKHEVEYGDDDDRGRFVPE"
     gene            complement(4274..>4897)
                     /locus_tag="VGR29_03385"
     CDS             complement(4274..>4897)
                     /locus_tag="VGR29_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2072662.1"
                     /translation="ERTYGAAYDQIRHSLERLRTDQVDLIQLHALHTEEEWQTAFSED
                     GALKAAIQARDEGLVRYIGVTGHGVEVARFHIRSLEQFDFDSVLLPYNFPMMKNEQYA
                     ADFEELVTICKERNVAVQTIKAITQAPWGDREQTAATWYEPLRDQDAIDTAVSWVLGR
                     EGVFLNTVGDIHVLPKSLDAAERFTSRPSNEEMTRLVESWQMEPLFT"
BASE COUNT          974 a         1556 c         1436 g          931 t
ORIGIN      
        1 ccgccatacc cacctttgcg gcgcgagtgc ccttcggaga gccgtccttc cacccgcccc
       61 tcacgtcatt gagggcaggc attggcgcgt agaggttgcc tgtagttgaa ggtgaaggtg
      121 tgtccgccag atgctcccgg tccaccatgg tcgccgaagc ccgctccgtc atcgccggca
      181 tgaacttcat ctgcatattc atcagccggc cgatgttccc gacgtagacc tcacgcttgg
      241 gatgctgggc catctcaacg atcatccgcg cgacattttc gggcggatag acgggcggca
      301 ttgccttcgg ggtgcgcccg gtgtaattgg ccgcatgctg aaagaacggc gtatcgatgg
      361 tagccggcaa cacggtactg acatgcacgt ttttctgctt gtccagcacc aactcctgcc
      421 tcaggctcat ccccagcgca cgaaccgcgt gcttgctcat tgtgtaggcg ctggtgtagg
      481 gctgcggcat gcgtgcaacg agcgacgaaa cgttgatgag cacgccgctt ccctgctccc
      541 ggaacaccgg gatcactgca cgcgcgccgt ggacataccc gaagacgttt gtctcgaaca
      601 cccgccggaa gtcctccatc ggcacctcat ggagctttcc aaagaatgag acggcggcgt
      661 tgttcaccca gacatcgatc cggccgaacg cgtcaatggc gcgttgggaa agcgccagaa
      721 cggccgcctc gtcggtcaca tcggtcggca cggcaacggc cttcgctccg cgctgctcgc
      781 actcggcaac gacctcctgg agctcggatt cgcttctcgc ggcgagcacg agggatgagc
      841 ccttgttggc gaactcatgc gccgtggcgc gtccgattcc gcttgaagca ccggtaacaa
      901 cgactacggc ttcctgcaac ttgcggggca tgggctcact ccttgtgtcg cagacatgtg
      961 gcgatgatca agaccgacca tcttgtggtc gcagggctcg gcagaacgcg gacgagcacc
     1021 atcatgctgc tgaggactat gcaagggata tgccagactc ggcctcttga ggaaccgctc
     1081 ggttttcccc gagcggcggg accccgacaa catgcgataa gcgaaccggt gatcggcatg
     1141 cttcttgcat ccgcgccact acggaccaat cgtccgggaa cgatgcacat aaggagaaaa
     1201 ccatggacgc cagtgagatg cgagaacttt tgcatggcta tctgagtgag atggattggg
     1261 cgggcgatac gaccaagaac gacattctgg agcatcttgc cggccgggat gaagccctgc
     1321 gcacgatggt caacgagtac gtcgcactgg gctcgtacca gggtccggat gccgttctcg
     1381 atctcctccc ggcccaggca tgggaggatg ttcagggaga cgaatggcgc ggcgccgaga
     1441 gccagtacgt cgaggatgtc gacagcaact atcaggaagg cccactgggt cagaaccagg
     1501 gcaacgccgg ctaaccacct cccctgcccc ggcttcaacc agtctgcccg agtttcccgc
     1561 gatccggtga cacaatccgg gaaccggggc cagcaacggc catacagcgg gagctattgg
     1621 ctcccgctgt ttgcatgtcc gaaatacctc taagactgct gaccggcaaa gtttattcag
     1681 aacggttttc ggtgaggttg catctccgta gcgaaggggt cgctgcctgg cgggcttggg
     1741 gcatcaatct cattgcgaaa tctagggcgc cggcgtctcg ctcgctctct cttcaccggc
     1801 cagaaaccac tcaacagcct cacgagcaat cttgcctggc acggtgtgcg gcccgtcgaa
     1861 ctcccggtac ctcacctcgt acccggcatc ttgcagaacc ggcacgacac gacggctggt
     1921 gtagtcgatg cgcaggacct catcccttat gccgtgggag acgaagatgc gcggctcgcc
     1981 ctgctgtggg cctggcactg cgaagccggg cgagaatgcc aggatatttg gaaagaggtc
     2041 gccgttcgta ataccaatcg agagcccgta ggatgcgcca tccgagaagc ctgacactgc
     2101 gatccgagcc ggatcgacag cgcaacgctc gaacgttgcg gcaagagcac gatcgagaaa
     2161 ggcgatatca ggcccaaacc tgccaagaat cacatcccat gtgcgacccc gcgatgccgg
     2221 cgagagcagg atgaatccga attcatcggc aagtacctgg agcgggtaaa gaccggacag
     2281 ctcgtttcct ccggccccat ggagtgagag aacgaaggga gccggctgct ccggctggta
     2341 tccctcagga atgtagagag ctgcgtcgcg atcatcgcca agaccgagtg gatgcatccc
     2401 cggggcaata cgctcagtcg tcgtaccggg acgcgccagc aaacggcctt ctcccgagtt
     2461 gctccctctc ctgtttctcc tcatatgttt catattcctc tctcgcgcct gtgcatgttg
     2521 aatggccaga gcgccgcctg ccatcgatgc gctcatcagc aggtgtcttc tcgtccatcg
     2581 accggcgtac cctgttccgc gtcccatcgt gccgtatcct ctggcatccc cgtgtagagc
     2641 cccatgcatg aagtgggtgc tacgccaatc ccgaccggat cttggcttga ccggcgcaag
     2701 agccggaccg ggactgctga ttccgcgtga ggcgcagtcc cttgaaagcc gtgctgtgat
     2761 acgctcacgc tcgtagccgg ctcggaacgt tcgcctttta cccgggactc atcaatgaca
     2821 tacacccctg acgaccaacg ctacgacgcg atgcgttacc ggcgcacggg ccgtagcggc
     2881 ctcctcctcc ccgaaatatc gctcgggctc tggcagaact tcggtgatac acgccctctg
     2941 gagacgcaac gatccatcct ccggcgggcc ttcgatcttg gtgtcaccca cttcgacctg
     3001 gcgaacaact atggcccccc gtatggcgcc gccgaggaga acttcggccg catctttgcc
     3061 caggacttcc gaccttggcg ggacgagatg atcatctcta ccaaggctgg atgggatatg
     3121 tggcccggcc cctacgggga tcacggctcc cgcaaatacc tgctcacctc gctggaccag
     3181 agcctgaagc gcatggggtt ggagtacgtc gacatcttct atcaccaccg gccggacccg
     3241 gaaacgccga tcgaggagac ggccagcgcc ctcgcgaccg ctgtccagag tggcaaggcg
     3301 ctctatgccg gtgtctccag ctacgacgcg gatcaggtcg gtgcgatggt cgaggcatta
     3361 gcggagcata agatccgcct gctaattcat cagccgagct acaacatgtt caaccgtcag
     3421 atcgaggatc gcctgctccc cctgctgaag gaggagggca tcggccccat cgtcttcgtc
     3481 ccgcttgcgc aggggttgct cacggaccgt tacctgaaag gggacattcc cgcggattct
     3541 cgcgcggcaa cgagcccgac actttcaggt gggatgatca cggaggccta cctcgagcgc
     3601 gcccgcgcct tgaacgagat tgccaggaac cggggacaaa ccctcgccca aatggcgctc
     3661 gtgtggatcc tgcgtaaacc gcatatcacg tccgctctca tcggtgcgtc aagtgttggc
     3721 caattggaag agaacattgc cgcgctcaat tctgctccgc tgagtgagga cgagctggcc
     3781 gccattgagc cgcacgctac cccgctcacc tgagatcgcg gcatgctccc tgcaattaga
     3841 aagggacaca cctgctttcc atcccttggt tcacggcagg tgcacgtaaa ggagtcgatc
     3901 atgaccgatg atgtccagcg tgagatcgag gccaacgagc ggcgcgcccg cgaccagcag
     3961 gatgtaggaa tagaggagga cgcgctcagg ggcaacgacg acagcacatt tctcgacgat
     4021 gccttggacg gtatccccgg gataggcgac gacgagtccg acaagcacga agtcgagtac
     4081 ggcgacgacg acgatagagg acgattcgtt ccggaatagc ctgcccggaa tgagtgacga
     4141 ctcacaggtt gtcgccctcc tctgcgctcg ctccgccgag cacacgatgt ttagctaaag
     4201 gtaaaggcga gcggaggcca acttccttgc cggctgttgg cctccgctct tgctcgctat
     4261 tgagatcgtg gccctaggta aacagcggct ccatctgcca actttcgacc agcctcgtca
     4321 tctcctcatt gctcggccgg gaggtaaacc gctccgcggc gtcaagagac ttcggcaaga
     4381 cgtggatatc ccccacggtg ttgaggaaga cgccctctct cccgagcacc caactgacgg
     4441 ccgtatcgat cgcgtcctgg tcccgtagcg gctcatacca ggttgcagcg gtttgctccc
     4501 ggtcacccca gggcgcctga gtgatcgcct taatcgtctg gaccgcgaca ttccgctcct
     4561 tgcagatcgt caccaactcc tcgaagtcgg ccgcgtactg ctcgttcttc atcattggga
     4621 agttgtaagg cagcagcacg gaatcgaaat cgaactgctc aagcgagcgg atatggaagc
     4681 gggcgacctc gacgccatgt ccggtgacac caatgtaacg aaccagcccc tcatctcggg
     4741 cctggatggc ggccttaagt gccccgtcct cactgaatgc ggtctgccac tcctcctcgg
     4801 tgtgcaacgc gtgaagctgg atcaggtcga cctggtccgt tcgcaagcgc tccagcgagt
     4861 gccggatctg gtcgtaggcg gcaccgtatg tccgctc
//
LOCUS       DASYWM010000081         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_115501, whole genome shotgun sequence.
ACCESSION   DASYWM010000081 DASYWM010000000
VERSION     DASYWM010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115501"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(557..2743)
                     /locus_tag="VGR29_03390"
     CDS             complement(557..2743)
                     /locus_tag="VGR29_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV2072663.1"
                     /translation="MIAFWLILVGLVLPLVPDIDGPLKVGGFSSDESEGARATQILEE
                     ELDFSSSQLAIIFASDTLKASDSSFLEQVDASLNEINRLDFVDDVITPSFDPTLIASS
                     GRLAYAVVGLGVPVEEAQAEARAVQEALIEQPDLDVLVAGGPSFYADVEVASQRDLQR
                     AEFIAFPVALIALIIVFGSLVAAAVPLLVGSAGVAVALLGLYWTAHIADMSIFVLNLT
                     TMLGLGLAVDYSLFVTSRYREELARRGGDVDESVVTAMSTAGRAVFFSGLSVLVGLSG
                     LSLFNLMFLRSVGIAGVIVVAISTSAALTLLPAILKIIGPRINALPMGRLARRHATEQ
                     VTTGFWYRLAKRVEQRPGLVTAGVLVILVLLGSPFLRANISSPDATMLPEDLPSRQGY
                     DLLASEFSGGEISPYIILIQSTGDMRSPESLQVIDSIGSTLVNDERIDHVQSVLTVPG
                     APADATPEQRLQLRERAEQLGIDTRLDSLLGDGTAMILAYPVAPANSEQNKQLLTELR
                     SWDAGEGYTVMVAGGTAEIVDVVNVIYSEFPLVALAIVATTFVILTMLFRSLVLPLKA
                     IIMNTMSILAAYGALVWIFQDGHLHEFLGFTPQGYVEASLPVIVFCVLFGLSMDYEVF
                     LLSRIQEEWLRTGDNDEAVALGLERSGRIISSAALIVVVVTASFVTADVVIVKALGLC
                     IALAVAIDATIVRALLVPATMKLLGHWNWWMPGWMNRVLPRINVGE"
     gene            complement(2819..3190)
                     /locus_tag="VGR29_03395"
     CDS             complement(2819..3190)
                     /locus_tag="VGR29_03395"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEV2072664.1"
                     /translation="MRYAASTECREKGVTYHLVPSEVWDRQSDAPTYLPEGYDRDGFI
                     HCTNGLDELVAVGNRYYKADERQFRVLILDLSKVESPVRYDDPDEIFPHIYGPLNINS
                     IVGLLLARRQEDGTFVSDTRE"
BASE COUNT          734 a         1014 c          884 g          616 t
ORIGIN      
        1 tgccatgact cgccagcgcg gctgagggta atgagatctc ggccccgtcg ccacatgaat
       61 tctgccgccg cctcatcaat tcgatccatc gccatcgtga ggactccagg ccagcgcagc
      121 ttgacacggg ggaagccagg ccgacgttgc ccaatactaa gttcaaatgc cactaatcct
      181 gcctcgtctt gcgcgagtta tcacaggatg ggacaggact cgcgtcgctc tccatccaga
      241 gcgcggaaat caatgtccgg gactcttgtg agtacccgat tccgctcttg ttctcggcag
      301 ctaccggggc caagcttcgt tgccggaatc cccaatacaa tcgtctgttt tgtcggtgct
      361 cgacgtagcc cacgccagcc acgtggattt cattacggag cttgtgcagt ttgcatgttt
      421 accgttcgct atacaggcgt atagcggacc gcagacaata gtacccgatg cacggccccg
      481 gattcactct ccccgtcccc ttttcaacgt ccctttcact gcacccgcgt tcaaaccatc
      541 gaacgttcgc aggtcattac tccccaacat tgattcgcgg aagaacgcgg ttcatccatc
      601 ccggcatcca ccagttccag tgtcccagga gtttcatcgt tgcgggcacc agcagcgcac
      661 ggacgatcgt cgcatcgatg gccaccgcaa gcgcaataca cagcccgagc gccttcacaa
      721 tcaccacatc ggcggtgacg aatgatgcgg tcaccaccac cacaatcaac gcggcactcg
      781 atatgatccg cccgctacgc tccagtccga gcgcaacggc ctcatcgttg tcccccgtac
      841 ggagccactc ctcttggatg cggctgagga gaaagacctc ataatccatg ctgagcccga
      901 aaagcacgca aaacacgatg accggcaggg atgcctccac gtatccctgt ggtgtaaagc
      961 cgaggaattc gtgaaggtga ccatcttgga agatccatac aagcgcgcca tatgcggcca
     1021 ggatggacat ggtgttcatg atgatggcct tgagcggcaa gaccaacgat cgaaaaagca
     1081 tcgtcagaat cacgaaagtc gtggcgacga tagcaagcgc tacgagcgga aattcggaat
     1141 agatgacatt cacgacatcg acgatctccg cggtgccacc cgcgaccatc acggtgtaac
     1201 cctcacccgc atcccatgaa cgaagttccg tgagcaattg tttgttctgc tccgagttag
     1261 cgggcgcaac cggatacgcg aggatcatgg ccgtcccatc gcctagcaac gagtccaacc
     1321 gcgtgtcaat tcccagctgc tcagcgcgct cccgcaattg cagccgctgc tccggagtgg
     1381 catccgcggg cgcacctgga acggtgagca cgctttggac gtggtcgatc cgctcgtcgt
     1441 tcaccagtgt cgacccaatc gaatcgatga cttgaagcga ttccgggctc cgcatgtccc
     1501 cggttgactg gataagaatg atgtacggcg atatctcgcc accgctgaac tcgctcgcga
     1561 ggagatcata cccctggcgg gatggaaggt cctcgggcaa catcgtggcg tccggcgacg
     1621 agatgtttgc cctgaggaag ggagacccca acaggaccaa aatcactagc acaccggccg
     1681 tgacgagacc cgggcgctgc tccacccgtt tcgcaagtcg gtaccagaag ccggtcgtca
     1741 cctgttccgt tgcatgcctt cgcgcaagac gtcccatcgg gagagcgttg atgcgaggac
     1801 caatgatctt caggatggcc gggagcagcg tgagagccgc cgaggtcgag attgcgacca
     1861 cgatcacgcc ggcgatgcct accgacctca ggaacatgag attgaacaac gataatccgg
     1921 acaggcccac aaggacactg agtccggaaa agaaaacggc gcgccccgcg gtggacatcg
     1981 ccgtcacgac ggactcatca acatcgccgc cgcggcgcgc caattcttcc cggtacctgc
     2041 ttgtgacaaa gaggctgtag tcgacagcga gtcctaggcc cagcatcgtc gtcaggttga
     2101 gaacaaagat gctcatgtcg gcgatgtggg cggtccagta cagtccgagc agcgcgacag
     2161 cgaccccggc actgcccacg agcaacggta cagctgccgc gaccagcgac ccgaagacga
     2221 tgatcaatgc aataagggca accggaaacg cgatgaactc tgcccgctgc aggtcccgtt
     2281 ggctggcaac ctccacatcg gcatagaatg acggccctcc ggccacaaga acatccaggt
     2341 caggttgctc gatcaacgcc tcttgcaccg cccgtgcttc cgcctgggcc tcctccactg
     2401 gcacgcccag gcctaccacc gcatatgcaa gtcggccgct tgaagcaatg agcgttggat
     2461 cgaatgatgg ggtgatcacg tcatcaacga agtccaggcg gttgatttcg ttcagtgaag
     2521 cgtcgacttg ctcgagaaag ctggagtcgc tggccttgag cgtgtcactc gcgaagatga
     2581 ttgcaagctg tgaggagctg aaatccaact cctcctcgag aatttgcgtt gcccttgcgc
     2641 cctctgattc gtcggaagag aatcctccca ccttcagggg accgtcaatg tctggcacaa
     2701 gaggcagtac gagcccgaca aggatcagcc agaaagcaat caccaaccac ggtcgcctgc
     2761 aggcagaccg tgccagatat tcgaacacta aaccaccgtc ccggcttcga ctctcacgtt
     2821 actccctagt gtcggacacg aacgtgccgt cctcctggcg ccttgcaagg agaagcccga
     2881 cgatggagtt gatattcagc gggccataga tgtgggggaa aatttcatct ggatcgtcat
     2941 atcgaaccgg cgattcaacc ttggacagat caaggataag cacacggaac tgtcgttcat
     3001 cggctttgta gtatcggttg ccgactgcaa ccagctcatc gagaccgttg gtacagtgga
     3061 tgaagccgtc ccggtcatag ccctcaggga gatacgtggg cgcgtcgctc tgacgatccc
     3121 atacctcgct ggggaccagg tgataggtga cacccttttc acggcattcc gtgctggcag
     3181 catatctcaa tgaccgctcc ctcaccgacc gggtgcctat cggtgcccat agccgcgaca
     3241 tccatcac
//
LOCUS       DASYWM010000082         5479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_378134, whole genome shotgun sequence.
ACCESSION   DASYWM010000082 DASYWM010000000
VERSION     DASYWM010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378134"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VGR29_03400"
     CDS             complement(<1..914)
                     /locus_tag="VGR29_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HEV2072665.1"
                     /translation="MSEYRNRSNRGSRDDEPPFDALAIDEESALEETPLDERLAALGD
                     LVNGDDALEDPDDLVGDLVDVDDEELADLRNTVSADAIAESDLAGVSLDDPVRMYLRE
                     IGRVPLLSAGREVELSTAMDLGEYVGKVRAHLSAVSGDEPSATEIGMTVWFAFKAGWS
                     LARDLWQELEPDNPPGSFQQVFERVLPITGVDGAAIKTVCTRYGISAASLEEQLRLRT
                     VEWGLLHESIQGIVSPNGPWPDDGAIRALWERRALRQARRWRELEQAGRQAKVALAEA
                     NLRLVVSVAKKYMGRGLGMLDLIQEGNLG"
     gene            complement(945..2282)
                     /gene="lysA"
                     /locus_tag="VGR29_03405"
     CDS             complement(945..2282)
                     /gene="lysA"
                     /locus_tag="VGR29_03405"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV2072666.1"
                     /translation="MSTQRAEHMSHVWPFGTHRNERGELMIGGVSVAEIAAEHGTPVY
                     AYDEATLRESMRRYRDAFTRELPDCRVVYAGKAFLSTPFVRVLVEEDLGLDVVSGGEL
                     FVALRGGMPPSRISLHGNNKSRDEIQMALEAGIGKIIVDNFGEIEMLAELCAGRKTPM
                     TVLLRVNPGVDVHTHKKISTGMADSKFGLPIIDGQAARAVAQLSATPGVHLAGYHAHV
                     GSQLFDAEASLDAIGELLDFSASMRSEYGVELEQLSPGGGFGIPYLDGDKPPVPERWA
                     EMIANAVRIGCDERGLPIPVVVIEPGRAIAGPAGVAIYRVGSRKELPGVRTYISVDGG
                     MADNIRPSLYDAAYTAALANRAGGEDRESVTIAGKYCESGDVLIENVRLPRLQRDDLL
                     AIPAAGAYCLAMASNYNMSLRPAVVMVSNGETHLMRRRERLDDLIRLDVLSSI"
     gene            2481..3458
                     /gene="rarD"
                     /locus_tag="VGR29_03410"
     CDS             2481..3458
                     /gene="rarD"
                     /locus_tag="VGR29_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEV2072667.1"
                     /translation="MGSVPVSPPHAGVVQRDAAAREVRLGTTYGFLAYASWGVLPLYF
                     HALAPAQAFEILAHRIVWCLIFCLVLVTLNRDTSWVPIILRHPQRLLLLTLAAFLLAI
                     NWGVYIYAVTSGNVVESSLGYFINPLILVLMGVFILHERLRRLQWWAVGLGTIAVLVI
                     SFNYGRLPWIALTLACSFATYGFIKNRVGANIGALPSMTVETVVLVPFALAGIIWIER
                     SGNGTFTSEGFGHTLMLLSTGIVTAVPLILFAAAARRVALATMGLLQFFAPVLQFICG
                     VSLLGEVVPLSRWIGFGLVWLALVVLTADTVRTSRSRRRVSIQTAGNTA"
     gene            3647..>5479
                     /locus_tag="VGR29_03415"
     CDS             3647..>5479
                     /locus_tag="VGR29_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV2072668.1"
                     /translation="MPPLSILTVSNLSKTYITDEIFANVSFQIQEREHVALVGVNGAG
                     KSTVLRIIAGIEYPNSGEVASAAGLRVTYLPQEARFTSDNTLREEARLAFAPVLEMQE
                     RMREIEHRMADTDADLDILMEEYDRIGVRFEAAGGYDVEHRTDEVLQGLGFSQDQFDD
                     PVARLSGGQKTRVALAKALLADPDLLLLDEPTNHLDLEMLEWLEEFLRRWTGACLIVS
                     HDRYFLDRVTTRTLDLSFGRLEDYPAPYAKYLPMRAEREARRLKEYQEQQEFIARQEE
                     FIRKYGAGQRYREARGRQKKLDRLERIDRPQEHDAMHLRLGTNLRSGRNVLTSSRLNI
                     GYRDPDGPAVLIRTPELTIERGDRIGLLGPNGAGKTTLIKTLVGALPVLKGEYNVGTN
                     VKPGYYAQSHEQLRGKGNGTPLSLILDTQSMSEEYARTWLGRFLFSGDDVYKHISDLS
                     GGERSRLALAILLLEQANFLILDEPTNHLDIQARETLEEMLNDFDGTILFVSHDRYFM
                     DRIATKLWIVEDGGITFSLGNYTDYQRHLGRRAQQEAAEAKQETKPQRAPDPVEEING
                     AKGKPRRRSESDVQKRLAKVERSISQLEGRLNELNDGLSIATIDQ"
BASE COUNT         1064 a         1743 c         1583 g         1089 t
ORIGIN      
        1 ccgaggttgc cctcctggat cagatccagc atgcccaggc cacgacccat gtatttcttg
       61 gcgacggaga cgaccagccg aaggttcgcc tcggccaggg ccacctttgc ctgacgaccc
      121 gcctgctcca actctcgcca gcgacgcgcc tgccgtaacg cccggcgctc ccacagcgcg
      181 cggattgccc catcgtccgg ccatggaccg ttgggtgaaa cgattccctg aatggattca
      241 tggagcaatc cccactcgac cgttcgcagg cgaagttgct cttccagcga ggctgccgat
      301 atcccgtacc tggtacagac cgttttgatc gcggctccgt ccaccccggt gatcggcagc
      361 acacgttcaa acacctgctg gaaggacccg ggcgggttgt ccggctcgag ttcttgccaa
      421 agatcccggg ccagcgacca acctgccttg aacgcaaacc agaccgtcat gccgatctcg
      481 gtcgctgacg gctcgtcacc acttacggcg gaaagatggg ccctgacctt gccaacgtat
      541 tcaccaaggt ccatcgctgt agagagctcg acctcgcgcc cggcggacag gagcggcacc
      601 cgaccgatct cccgcaggta catgcggaca ggatcgtcga gactgacgcc agcgagatcg
      661 ctctcagcga tggcatccgc ggagaccgtg ttccggagat cggccaactc ctcgtcgtcc
      721 acgtcgacga gatcgcccac gaggtcgtcc ggatcctcta gagcatcatc tccgtttacg
      781 agatcgccga gggcagcaag acgctcatcg agcggcgtct cttccaacgc ggactcttcg
      841 tcaatcgcga gggcgtcaaa cggtggctcg tcgtcccggg atcctcgatt ggaccggttt
      901 cgatactcgc tcaaacgcga atctcccgtt gcacgaacga tgttttagat actggacagc
      961 acgtccagtc gaatcaggtc gtcaagcctc tcccggcgtc gcatgagatg cgtttcgccg
     1021 ttgcttacca tcactactgc cggtcgaagg gacatattgt aattgctcgc catggctaag
     1081 caataggcgc ccgctgctgg aatggccagc aggtcgtctc gctggagccg tggcaagcgt
     1141 acattctcga tgagaacgtc tccggattcg caatatttcc ccgcgatcgt cacgctttcc
     1201 cggtcctccc cgccggcgcg attggcgaga gcggctgtat aggcggcgtc gtaaagtgaa
     1261 ggacgtatgt tgtcagccat cccaccatcg acggagatgt acgtccgcac tcctggcagc
     1321 tccttgcggg agccgacccg gtagatcgct acaccggccg gccctgcgat tgcccgtcca
     1381 ggttcgatca cgaccactgg aataggaagg ccccgttcgt cacagccgat cctgaccgcg
     1441 ttggcgatca tctcggccca gcgttccggt acgggcggct tatccccatc caggtacggg
     1501 atgccgaagc ctcctcctgg tgacagctgc tccagctcga caccatattc ggaacgcatt
     1561 gacgccgaaa aatcgagcag ttcaccaatc gcatcaaggg acgcctccgc atcgaacaat
     1621 tgggagccaa cgtgtgcgtg atacccagca agatgcacgc ccggcgttgc cgacagttgc
     1681 gcgacggccc tggctgcctg cccgtcgata attggcagac cgaacttgga gtccgccatg
     1741 ccggtactaa tcttcttgtg tgtatgcacg tcgacacccg gattaacccg caggagcact
     1801 gtcatcgggg tcttacgtcc agcgcagagt tccgccagca tctcaatttc gccgaagttg
     1861 tcgacgatga tttttccgat ccctgcttca agtgccatct ggatctcatc gcggctcttg
     1921 ttattgccgt ggagggagat gcgactggga ggcatgccac ccctgagggc gacgaaaagc
     1981 tcgccgccag agaccacgtc cagccccagg tcttcctcaa ccagtacacg gacaaaggga
     2041 gtcgacagaa acgcttttcc cgcatacacc acccggcagt cgggaagctc acgcgtgaag
     2101 gcgtcacgat accggcgcat gctctccctg agtgtcgctt catcgtaggc atagactggc
     2161 gtgccatgct cggcagcgat ctcggcaacc gagacaccgc caatcatcag ctcgccccgc
     2221 tcattgcggt gcgtcccaaa cggccatacg tgcgacatgt gctcagcacg ctgcgtgctc
     2281 acctcgacct gtcctcctgg cggtgcagtt cccataccgg gcattctcgc acaatagtcg
     2341 tcgagattgg agggcagagt gagggctcta cagcgcgggc ccgttacaga gcaagagcgc
     2401 atggaccacg ctcccaccgt acaatgcgct aggtgcgatc gcctgtcgtc gcgctatgaa
     2461 ttccatcaag gaaccttgtt ttgggatcag tccccgtttc tcccccgcac gccggagtgg
     2521 ttcagcgtga tgccgctgca cgtgaagtgc gcctgggcac cacgtacggc ttcctggcct
     2581 atgcgtcctg gggagtgctt ccactctatt ttcacgccct cgcgccggcg caagcattcg
     2641 agattctcgc ccaccggatc gtctggtgcc tgatcttttg cttggtccta gtcactctga
     2701 atcgggacac ctcctgggtg cccattattc tccgtcatcc acagcggctg ttgctgctga
     2761 cgctggcagc cttcctcctg gcgatcaact ggggcgtcta catttacgct gtgaccagcg
     2821 ggaatgtggt ggaaagttca ctcggatact tcatcaaccc gctgatcctg gtgctcatgg
     2881 gggtcttcat cctgcatgag cggctcaggc ggctgcagtg gtgggcagtt ggactcggga
     2941 cgatcgcggt cctggtcatc tcgttcaact atggacgcct gccctggatt gcgctcacgc
     3001 tcgcgtgctc ctttgccacc tatggattca tcaagaatcg cgtcggcgcc aacatcggtg
     3061 cgctcccaag catgaccgtt gagaccgtcg tgctcgttcc gtttgccctg gcaggaatta
     3121 tctggattga gcgctccggc aacggcacct ttacctcgga aggtttcggg catacgctga
     3181 tgctcctgtc caccggtatc gtgaccgccg tgccgctgat cctcttcgcg gcggcggccc
     3241 ggcgagtggc actggcgacc atggggctat tgcaattctt tgccccggtg ttgcagttca
     3301 tctgcggggt ttcccttctc ggggaagttg ttcccctcag tcgctggatc ggattcggac
     3361 tggtctggct tgcgctcgtc gtgctcaccg cggatacggt ccggacgagt cgctcccgtc
     3421 gccgagtatc gatccaaact gccgggaaca cggcctgaca ctggtatact tatggctggc
     3481 aacagttcgg acggtgcccc acccccacac ctgctcatgg aacgatcgtc gtgacacaac
     3541 ggacacagtg acaaccttca tatcaaatcc gaagcgctga tcgcgtcctg cgagcagtcg
     3601 ttccgtgttg tcgtgcccgc ctcatcccga catctgaaag gccatcatgc caccgctgag
     3661 cattcttact gtttccaact tgtccaagac ctatatcacc gacgagatct tcgcgaacgt
     3721 ctcattccag attcaggaac gggagcatgt cgcactggtg ggcgttaacg gcgccggcaa
     3781 gtcgacggtc ctccgcatca tcgccgggat cgaatacccg aactccggtg aagttgcgag
     3841 cgcggcggga ttgcgtgtca cctatctccc gcaggaggcg cgattcacca gcgacaatac
     3901 tctccgcgaa gaagcccggc tggcgtttgc ccctgtgctg gagatgcagg agcgtatgcg
     3961 cgagatcgag caccggatgg cggacactga cgcggatctc gacattctca tggaggaata
     4021 cgaccgcatc ggtgtccgct tcgaggctgc tggaggctat gacgtcgagc accgcacgga
     4081 cgaggtcctg cagggattgg gattctccca ggaccagttc gacgacccgg tcgcccgcct
     4141 gagcggtggc cagaagacgc gtgttgcgct cgccaaggcg ctgctcgccg accctgatct
     4201 gctcctgctc gacgagccaa cgaaccacct tgatctggag atgctggagt ggcttgagga
     4261 gttcctcagg cgctggaccg gtgcctgcct gatcgtttca cacgatcgtt atttcctgga
     4321 ccgggtcacg acccgcacgc tcgatctctc gttcgggcgc cttgaggact atccggcgcc
     4381 ctatgcgaaa tatctgccaa tgcgcgccga gcgcgaggcg agacgtctca aggaatacca
     4441 ggagcagcaa gagttcatcg cgcggcagga agagttcatc cgcaaatacg gtgcgggaca
     4501 gcgctatcgc gaggcgcgtg gccggcagaa gaaactcgac aggctggagc gcatcgaccg
     4561 gcctcaggag catgacgcta tgcacctgcg attggggacg aacctgcgct cggggcgcaa
     4621 cgttctcacc tcctccaggc tgaacatcgg ctaccgggat cctgacggac cagcggtcct
     4681 catcaggaca ccggagctga ccatcgaacg tggtgaccgg atcggcttgc tgggccccaa
     4741 tggcgccggc aagacgacac tcatcaagac cctcgttggt gcgcttccgg tgttgaaggg
     4801 cgaatacaac gtgggaacga atgtcaagcc cggctactac gcgcaatcac acgagcaact
     4861 gcgcggcaaa ggcaacggca ccccgctctc attgattctt gatacacagt cgatgagcga
     4921 agagtacgct cgcacatggc tcgggaggtt tctcttcagc ggcgatgatg tctacaagca
     4981 catctcggat ctctccggcg gtgagcgatc acggctggca ctggcaatcc tgctgctgga
     5041 gcaagccaat ttcctgatcc tggatgagcc gacgaaccac ctggacatcc aggcccgtga
     5101 gacgctggag gagatgctca atgacttcga tggcacgatc ctcttcgtct cccatgaccg
     5161 ctacttcatg gaccggatcg cgaccaagct ctggatcgtt gaggatgggg gcataacgtt
     5221 ctcactcggc aactacaccg actatcagcg gcatcttgga cgacgcgccc agcaggaagc
     5281 cgccgaagcg aagcaggaga cgaaaccgca gagggccccc gatcccgtcg aggaaataaa
     5341 tggggccaag ggcaagccgc gacgtcgttc ggagagcgat gtccagaagc gtctcgccaa
     5401 ggtggaacgc tcgatctccc agctcgaagg gcggctcaat gagctgaacg acgggctaag
     5461 cattgccacg attgaccag
//
LOCUS       DASYWM010000083         5678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_340715, whole genome shotgun sequence.
ACCESSION   DASYWM010000083 DASYWM010000000
VERSION     DASYWM010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340715"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VGR29_03420"
     CDS             complement(<1..639)
                     /locus_tag="VGR29_03420"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV2072669.1"
                     /translation="MNTDEITLHTGMPVPRQLITNVSTWAQHELTPGWLASLPETVAF
                     LCAKWNIELDPVIPDSYITLVLLGHSADLGPVVIKSSPLADEFRAEATALELAAGENV
                     ARRYDVVFGRSAMVMERIVPGTQLRDVAMTDEDATRLAAETVRTFWRPVPNPIGLHPL
                     QQWMRELFTWSPRPDRVPTELVEHAQEVGSSLLAQSSRSCLLHGDFQHHNVLQ"
     gene            1163..1426
                     /locus_tag="VGR29_03425"
     CDS             1163..1426
                     /locus_tag="VGR29_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEV2072670.1"
                     /translation="MAPRVINVRTKGQMTLPADICEELGLRDGGALYLKRREHEIVLI
                     PVDAVVDPTDGILAEYAYTRNPSPAEEREWVARHIAKSADDYE"
     gene            1754..2869
                     /locus_tag="VGR29_03430"
     CDS             1754..2869
                     /locus_tag="VGR29_03430"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEV2072671.1"
                     /translation="MSEVRVLVGTRKGAFILTSDEARKDWDVTGPHFPGFEIFHLKAS
                     PVDPNRIYASQASDWFGQIMHRSDDGGQTWETIGNEFAYDGETGTHQWYDGTPHPWDF
                     KRVWHLEPSLHDLDTVYAGVEDAALFKSTDGGQTWKELNGLRTHTTGNTWQPGAGGLC
                     LHTILLDPTNPDRIFVAISAAGAFRSDDGGETWMPINKGLRSDYIPDEDAEVGHCVHN
                     MAMHPARPGTLFMQKHWDVMRTDNAGDQWHEVSGNLPSDFGFPISVHAHEPETVYVVP
                     ITSDYHHFPPDGKLRVYRSRAGGNEWEALTNGLPQENCYVNVLRDAMSVDRLESCGVY
                     FGTTGGQVYASSDSGDNWTAIVHDLPAVMSVEAQTLS"
     gene            2866..3162
                     /locus_tag="VGR29_03435"
     CDS             2866..3162
                     /locus_tag="VGR29_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEV2072672.1"
                     /translation="MIRVLLPPHLQNLARTGKEVQVDVAGEATQRSVIDAIEAAYPML
                     RGTIRDHQTKERRPMVRFFVSERDLSHEPLDAPLPDEVARGEEPFWIVGAISGG"
     gene            3510..3746
                     /locus_tag="VGR29_03440"
     CDS             3510..3746
                     /locus_tag="VGR29_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072673.1"
                     /translation="MGRIIVANRHGHQLVEWDTTTDTEEARRAVADAERIIEEARANG
                     CAVSKKVNGEHVLDRGAFDPNAEEYQIVSPIAGG"
     gene            3875..4306
                     /locus_tag="VGR29_03445"
     CDS             3875..4306
                     /locus_tag="VGR29_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072674.1"
                     /translation="MSAQDPVELPWRHRWDHLPKRTAGFGFSQRDYEAARRRAEEVAR
                     TTLGESLWRQLRRDGYLELPSGIYQGVTYRLRIGRRIEVRCAPGVRAPWRHPYLCINP
                     TYPLPEAEFFAHLYLYVRDQEEEIIRVAAPQPWDQALGRTF"
     gene            4610..4870
                     /locus_tag="VGR29_03450"
     CDS             4610..4870
                     /locus_tag="VGR29_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEV2072675.1"
                     /translation="MALRASKVTRKGQVTIPIEVREKLNIQEGDTIFFEEREDHIAIV
                     RPQDLVERTSGVLKEYAKNFEGELPSRDEIWIGIAKERGTIE"
     gene            4863..5288
                     /locus_tag="VGR29_03455"
     CDS             4863..5288
                     /locus_tag="VGR29_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV2072676.1"
                     /translation="MNSKTNGAVLIDTNVFLRHLLDDIPEHSRRANKLFADVMKGDVR
                     VYAPSTVFFEAVYVLTRHKGVPVADVSAALHILLNYPGFETDHREALGGALSLWNTQG
                     PLSFADCFHLVLAKQLGMDRIYAFDGKMDRFPGVERVDP"
     gene            5378..>5678
                     /locus_tag="VGR29_03460"
     CDS             5378..>5678
                     /locus_tag="VGR29_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2072677.1"
                     /translation="MPSSPSTATYDSIADWYAAWVEQESWAHPTFLRHLFSLTGDVSG
                     SRVLDVGCGEGYFSRAVARADANVTGVDISERMLSLARTRTGADLPVEYLMDDARA"
BASE COUNT         1174 a         1712 c         1651 g         1141 t
ORIGIN      
        1 ctgcagcacg ttgtgatgct ggaagtcgcc atgaaggagg caggaacgtg aggattgtgc
       61 tagcagcgac gatcccacct cctgcgcgtg ctcaaccaat tccgtaggaa cccggtcagg
      121 acggggcgac caggtgaaga gctcacgcat ccattgttgc aacggatgaa gcccaattgg
      181 gttcggcacc gggcgccaga aggtgcgcac tgtttccgcc gccagtcggg tcgcgtcctc
      241 atcggtcatc gcgacatccc gaagttgcgt tccgggcaca attcgctcca tgaccatggc
      301 acttcgcccg aaaaccacgt cgtaccggcg cgcgacgttt tcgccggccg ccaattcgag
      361 tgccgttgcc tcggcgcgga actcgtcggc gagcggtgat gacttgatga cgactggacc
      421 gaggtcagcc gagtgtccga gcaacaccag ggtgatgtag ctgtcgggga tgaccggatc
      481 gagctcgatg ttccatttgg cgcacaggaa ggccacggtc tcaggcagtg aggcaagcca
      541 acctggcgtg agttcgtgtt gagcccacgt gcttacgttc gtgatcagct gacgtggcac
      601 gggcatgcca gtgtggagcg taatctcatc agtgttcatt gatgttccag gatcagcttg
      661 tccttccatc cgtttgggac ggaggtggca gcacgtaact ggctataacg ttcctgcagg
      721 aaagaccgaa caaagaaccg cattgccctt cgacaagatg gatatcgtac cgccaagcgt
      781 gcgctgacgg gggtacccca agcgctctac cagaaatcgt tgcggtacgt caccgcttgt
      841 tgccactcga gtcccggtcc gggatgacag aaaatagcac ctgcgaacta cctgtcggca
      901 aacacagcgg acgacttgcc acaggcaatg tcgtccctcc accctccctg accgacgtca
      961 ttcttcctgc actcgcacaa atcggaggaa cattcaggca cactcgccct tgtccctctc
     1021 tcagcgttcg attgttctgg cccatcgtgc caatcagcaa tctccagctt gaagaagttg
     1081 aatgaagctc tgcatgtatg acacatacca ataacttcac aggtaagatg atgtcgtcat
     1141 acaaccataa aggaggtcgc acttggcacc tcgtgtaatc aacgtccgga cgaagggaca
     1201 aatgacgctc cccgccgata tatgtgagga gcttgggcta cgtgacggtg gcgctctcta
     1261 ccttaagcgt cgtgaacacg agattgtgct gattccggta gatgctgtcg tcgacccgac
     1321 cgacgggata ctcgccgagt atgcctatac aaggaatccg agccctgcgg aagaacgtga
     1381 atgggttgct cggcatatcg cgaaatcggc ggacgactat gaatgagcag caggccgtcg
     1441 ttatcgatac caatgtcatt ctccgccacc tcctgaacga ccacgacgag cactcgccgc
     1501 gagcgtctgc cttgttcctg atggttcggc ttggagagca ctctgtccgg atctccggag
     1561 taagcgcaaa cccatctctt atgtatgcag catcctggat gcttgccttg atgcgtctgc
     1621 acattcacgt cctccagatc ggcggacgcc actcagccac accgtgcgca agatggaaga
     1681 agcgcccctg ctggagactt gctagaatcc tcgcatgatg tacgtacact cggcatatga
     1741 gtaggagcag gatatgagcg aggttcgggt acttgtcggc acgcgcaagg gcgcgttcat
     1801 cctgacatcg gacgaggcgc gcaaggattg ggacgtcaca ggtccgcact tcccaggatt
     1861 tgagatcttc cacctcaagg catcaccggt tgaccccaac cggatctatg cgtcacaggc
     1921 gagtgactgg ttcgggcaga tcatgcatcg ctcggacgat ggtgggcaga cgtgggagac
     1981 gatcggcaac gagttcgcct acgacggcga aaccggcacc caccagtggt acgacggcac
     2041 gccgcatcca tgggacttca agcgagtctg gcatctggag ccgtcgcttc acgatctcga
     2101 taccgtgtat gcgggtgtcg aggacgcggc actgttcaaa tccaccgatg gtggccagac
     2161 gtggaaggaa ctgaacggcc ttcgcaccca taccaccgga aatacctggc agccgggtgc
     2221 aggagggctt tgtctccaca ccatccttct ggatcccacc aatccggatc gcatcttcgt
     2281 cgccatctct gccgccgggg cattccgctc ggacgatggt ggcgagacct ggatgccgat
     2341 caacaagggg ctccgttccg attacatccc ggacgaggat gccgaggtgg gccattgcgt
     2401 gcataacatg gccatgcacc ccgcccgccc gggcacgctc ttcatgcaga agcactggga
     2461 cgtgatgcgc accgacaatg cgggcgacca gtggcacgag gtgagcggca acctgccaag
     2521 cgatttcggg ttcccgatca gcgttcacgc gcacgagccg gaaaccgtct acgtggtgcc
     2581 gatcaccagc gattaccacc actttccgcc ggacggaaag ctccgtgtct atcggtcccg
     2641 ggccggtggc aacgaatggg aagccctcac gaacgggctg ccccaggaaa actgctacgt
     2701 caacgtcctg cgtgacgcga tgtccgttga ccggctcgaa tcctgtggcg tctatttcgg
     2761 cacgacgggc ggtcaggtct acgcctcgtc cgactctggc gacaattgga cggccatcgt
     2821 tcacgatctt ccggctgtga tgtcggttga ggctcagaca ctgtcatgat tcgcgtcctc
     2881 cttcccccac acctgcagaa cctcgcccgg accggcaagg aagtccaggt ggatgtcgct
     2941 ggtgaggcga cgcagcgctc cgtgatcgac gctatcgaag ccgcgtatcc gatgctgcgt
     3001 ggcacgatcc gcgatcacca gacgaaggag cgacgcccca tggtgcgctt cttcgtctcc
     3061 gaacgggacc tgtctcacga gcctctcgac gccccgctgc ctgatgaagt ggcgcgggga
     3121 gaggagccgt tctggatcgt gggcgccatt tccggcggat aaaactggtg accgggagtc
     3181 gagccgagac agcaggccaa gtaaccacca taattagctc cagagcatgg gcagcgcgat
     3241 ggcctccttc aaattaagtc ctcaaggagg acttcgcggc attcgcaccc agcaacatcc
     3301 gcccttgtgg cacgtcctac ggcagaaccc ctggggagaa aatggcccgc cagcctgcag
     3361 ctcgatctgt ggcgggaccg ctacctcggg gaaagaaggg ggcgtgtcgc cgggagtgcc
     3421 cctcttgcca tctggaccct ccgggtgtag catggtggcc cgaccaagca accgtgcctt
     3481 gggggtcgtt tacggcaaag gagtccgcca tgggccgcat catcgttgcc aaccgtcacg
     3541 gccatcaact cgttgagtgg gacaccacca ctgacacgga agaggcccgg cgcgctgtcg
     3601 ccgatgccga gcggatcatc gaagaggccc gagccaacgg ctgcgcggtc tccaagaagg
     3661 tcaacggcga gcatgtgctt gatcgtgggg cctttgatcc gaacgccgaa gaataccaga
     3721 tcgtctcccc catcgccggc ggataacgct gccgttcccc gtcatgagtg aaggaaccca
     3781 ttcaccagac agaaagctgt ccggatcagc atcggggctc acgtggtggt cccgatgttg
     3841 gtcctggctt tcaggtcgcg aggaggagag cccgatgtcg gcacaggacc ccgtggaact
     3901 tccgtggcgg catcgttggg accacctgcc gaagcgtact gctggctttg gcttctcgca
     3961 gcgtgattac gaggctgctc gacgccgggc agaagaggtc gcacggacga cgctgggtga
     4021 atccctgtgg cgtcagctcc ggcgcgacgg ctacctggaa ctcccctcgg ggatctacca
     4081 gggtgtgacc tatcggctgc gtattggccg gcggatagag gtgcgctgcg cgcccggggt
     4141 ccgcgctccc tggcggcacc cgtacctgtg catcaaccca acgtaccctc tccccgaggc
     4201 tgagttcttc gcccacctct acctgtatgt ccgcgatcag gaagaggaga tcattcgcgt
     4261 ggctgccccc cagccatggg accaagcgct cggccgcact ttctagcaag gaacgtttac
     4321 gcctgaagga ctgctggctg gtagatcgaa caagaccgga tcctcccgta aaggcaagcg
     4381 gccgaagata gcaagagatt ttgatcggcg tcggcaccgc gcaatattgt gcctccctac
     4441 cctccccgca caccagatgc ggggctacat ctgcaggcga tgcagcagac ttccttgtat
     4501 gggtactcag ctcgctccac cctcccccat ccttcccaga atcaccttcc caccccttgg
     4561 tactacctcg tataattagg tagtaccagt aactaccaag gaggatacgg tggcattgcg
     4621 tgcatctaag gtcacccgta agggacaggt gaccatcccg attgaagtcc gggagaaact
     4681 gaacatccag gagggcgaca cgatcttctt cgaagaacgt gaggatcaca ttgcaattgt
     4741 tcgcccacaa gatcttgtcg agcgcacttc gggtgttttg aaggaatacg cgaagaactt
     4801 cgaaggtgag cttccatcac gtgatgagat atggatcggc atcgcaaaag agcgtggaac
     4861 aattgaatag caagacgaat ggcgcggtcc ttatcgatac gaatgtcttt ctcaggcacc
     4921 tgctcgacga tattcctgag cattcacgtc gggccaacaa gctttttgct gatgtcatga
     4981 agggtgacgt tcgagtttat gccccttcga cggtgttctt cgaagcagtg tatgtgctca
     5041 cccggcacaa gggcgttcct gtggcagacg tgtccgcggc actccatatc ctactcaact
     5101 acccaggatt cgaaacggat catcgggaag cgcttggcgg cgcattgtcc ctttggaaca
     5161 ctcaaggtcc tttatccttc gccgactgct ttcaccttgt cttagccaaa cagttaggca
     5221 tggaccgtat ctacgcattc gacgggaaga tggatcgctt ccccggcgtc gagcgcgttg
     5281 atccttaacc ggctccattc acatcctcat ccgaacccag ttgatcgggg ggctatcgaa
     5341 ttttctcctc gtgtgtgaat acgtcccgga gccatgcatg ccatcctcgc cctccacagc
     5401 cacttatgac tccatcgcgg actggtacgc agcgtgggtc gagcaagaaa gctgggcaca
     5461 cccgacattc cttcgtcacc tgttttcgtt gacaggcgat gtttcgggat cgcgcgtcct
     5521 ggatgttggt tgcggtgaag ggtatttctc ccgcgcagtc gcccgcgctg acgccaacgt
     5581 gacgggcgtc gatatctcgg agcgtatgct ttccttggca cgcacacgta ccggcgctga
     5641 tctgcccgtc gagtatctga tggatgatgc ccgggcac
//
LOCUS       DASYWM010000084        12339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_603539, whole genome shotgun sequence.
ACCESSION   DASYWM010000084 DASYWM010000000
VERSION     DASYWM010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603539"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="VGR29_03465"
     CDS             complement(<1..1367)
                     /locus_tag="VGR29_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2072678.1"
                     /translation="MTLRPSSLNLLDLQPSALPYPLQDGPRLPLPPTRLVGREHEVTR
                     AGELLRQSLVRLLTLTGPGGVGKTRLALEVAAELVDVFADGAAFVNLAPIHDSALVVP
                     TVAQVLGVRGGGSEAPAATLMEHLRGRRMLLILDNFEQMVEAAPIVADILASCPDLKV
                     LATSRVALNIRAEHVFPVVPLALVNPVRRTAGFALNASPAVTLFVERSRAVRPSFELT
                     TKNAAAVAEICARVDGLPLAIELAAAGIRILPPEALLARLHSRMDLLGSGPRDLPARH
                     RTMRDAIAWSYDLLDQNEQALFRRLSIFAGGFSLDAAEAVAGAGAGGEMAPVPPTFDV
                     VASLVTQNLVIQDSVAGQARFRMLETIREFGLERLDAAGETGLVRHQATTWCLRLAEE
                     AEQELIRGEQAKWLDRLEAEQDNMREALEWSLTCGEAETGLRLAAALWRYWSARGDLR
                     EGYSWL"
     gene            1836..4157
                     /locus_tag="VGR29_03470"
     CDS             1836..4157
                     /locus_tag="VGR29_03470"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV2072679.1"
                     /translation="MTAITTLQEQSTLLECVEQLISNDTPDEARSCREVLADHFEPEV
                     LAALENLSEAIEGNQHTPNTRMVARAFLEAYSAHQGMARKSGEPYIVHPVEVATILAR
                     MHLDTDTLAAGLLHDAIEDTPVTFNLIAEHYGERVARLVEGATKLGKIPWTPDSDDGR
                     ERVAREKEQQAESLRKMFLAMVDDIGVVLIKLADRLHNMRTLGAMPRHKQIRISQQTM
                     EIYAPLANRLGIWQVKSELEDLAFRYLMPEQYQAIANELEKRVEDSTSYLNAITAQLQ
                     GLLDQAGIKAEMVGRKKHIYSIYRKMNRKARPFDEIYDVIGVRILVETVQDCYSVLGI
                     VHGRWHPLPGEIDDYIATPKESMYQSLHTAVIGPDAHPVEIQIRTREMNQVAEYGIAA
                     HWRYKEGGKGDQRLEAKIAWLRQLMDWRDDVADAEEFVESLKSDVFQDQVYCFTPAGD
                     IIELPNGATPVDFAYRIHTQIGHQCVGATVNGQMVPLDYKLQNAQVVKIKTSKTVNGP
                     RRDWLQANSGYVHTASAREKIRQWFRRQERDENISQGKETLERELRRLALDMKPDDVL
                     ARFPQYTKLDDFLAAIGYGAVTAQAIATKLDETPKDVFPTTPVIRAPTTPARVEVMGA
                     GNLLTIQANCCRPVPGDEIVGYTTRGRGVVYHRADCTNIVNISDPERLVPVSWGGQVH
                     ETHPVPVRLIAMDRVGLLKDVSTLLSDERVNILSMLTQTHDDRSVTLLLTVEVESVEQ
                     LSRIMHRLENLRDVVEVRRDTGSITAINQVRAS"
     gene            4160..5101
                     /locus_tag="VGR29_03475"
     CDS             4160..5101
                     /locus_tag="VGR29_03475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEV2072680.1"
                     /translation="MPGPVIAIGECLVDLIAPGGVDLTVANELRIREGGAPANVAVAL
                     ARLDVLSQMRAVLGDDPFGERLRSRLNREGVDVSTIRFADGIPTTVAFAWSDERGNGR
                     FRIHRNADALLSPDDVTRESLNGAAAIVVGSVAMCAEPSKSAILSALRHALDLGVPVV
                     ADLNIRPGPGVPLDELRIIAAALVSSATVVKLSVDDSRELWGATSIREASTELDRFDP
                     AIAVITDGGRGAALRASGRLVELDVFPVEAIEPTGAGDAFTAAFISRMLAKDWAGADV
                     DDLRFAMAAGALATTEPGAMDGLPTRERIEDFLQLHA"
     gene            5179..6006
                     /gene="lgt"
                     /locus_tag="VGR29_03480"
     CDS             5179..6006
                     /gene="lgt"
                     /locus_tag="VGR29_03480"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV2072681.1"
                     /translation="MPSLHDPVAIALGPLEIRWYAIFILSGIFGAVALAHWLAGKRGL
                     DQEFIFDIAPWVVFLGIAGARLYYVLLEWDRFKDDLFGAINIRGGGLTIHGAVIAGAL
                     TVWWLCRRYREPLFTWLDIIAPGVALGQALGRWGNWANQEAFGTPTDLPWAVTIDPAR
                     RPSGYEAFATFHPTFLYESISNLVNAMLLSWLVVNGPRLKWFRDGDVIGLYLVAYGVV
                     RFFIERIRTDSLYIGPWPAAYWLSFGLIAAGLAIMVLRRRVPAETGSVIVASREDAA"
     gene            6003..7823
                     /locus_tag="VGR29_03485"
     CDS             6003..7823
                     /locus_tag="VGR29_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072682.1"
                     /translation="MMRRVPIIVCLVLMLASGGMLEQIAAAQSEGDVLDLANVPLTAD
                     ALPEPGYQILTGGYLDRDATVEQIASPRNAQVGDVEGMLINTGTVRAYVLNLVLLEDR
                     AHAGSDILSLVQTTVYLFVDVDGATDAFQVLSDYSSSADAEEAEPAIVGATTVRLSSQ
                     SGDTYRSVARSERAVIEVVSLETFDFVDADIHRRVVADSYDRLVDLQSLSSGGIAPQA
                     VLIGDGDNVTDLMAADGTGVHQLYRVREGEVQAAAGELRPPAPEEIAPGLTQLYVASQ
                     GVRIGQGTGFYSSWIGQFGSDPDAAAFVAGLPTSSSTALLPDPYYSLWADEQAVSQGV
                     TGVYRVSGAGDQGTFSGTLEIRQHGPYVVGIGWRTLGSVLPSVDVTSRLMDAQLTCLE
                     VPATCAPLPLEELLPVDPATPAIAVPGPDEAGSDEFGWSLPIDPQMWSTTEEFAEAGY
                     DFIELQSGRSLVTVESVIDQHGDPQQCVVDELRDLQDFEEHAVIELGSDVAGERPAGM
                     EAGHAWAVYTVEPLAEERADQEYTIRIDCYTLVEGSASLIMTHRAPRYEWEGERLKGE
                     ALRDALQLPSGKRRGGIITPQHPYNWRWIMIDKIWIGLAA"
     gene            7961..9337
                     /gene="hflX"
                     /locus_tag="VGR29_03490"
     CDS             7961..9337
                     /gene="hflX"
                     /locus_tag="VGR29_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV2072683.1"
                     /translation="MELHTQSTAGEPERALLVAVETPSQAWTASDSLEELASLAETVD
                     VEVVGSVTQKLGHPHPATYVGKGKLEEIKGLQEDLDYNLLLVDGELAPAQIRNLEQAL
                     EMKVIDRTALILDVFARRAQTHEGRLQVELAQMQYRLPRLTRMWSHLSRQGVGGVGLR
                     GPGETQLEADRREAQKRIAFLKGQMEQVHRHRELYRARRKDTPVSIVSLVGYTNAGKS
                     TLMNQITGAGVLAEDKLFATLDPTTRRITLPSGREVLLTDTVGFINNLPTQLIAAFRA
                     TLEEISEASMLIHVVDVTHQNATEQVQTVKETLEELGVDDRPVIYAMNKVDRLTKKQR
                     ADLREVAPKMGVPSDAIAISAQTGLNIEGLLNAVETVLEQEEGFVPVTARIPFNRSEL
                     VERFHTNGRVDERDFDENGTTLTGWLPERELGQFIPYVTLDGTHPNGHDESVLSDHSG
                     EVTANSVN"
     gene            9427..10503
                     /gene="purM"
                     /locus_tag="VGR29_03495"
     CDS             9427..10503
                     /gene="purM"
                     /locus_tag="VGR29_03495"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV2072684.1"
                     /translation="MDRPTSISYKDAGVDIDAKMQAIDRLKARAQSTLGASAGPIGHF
                     GGTWRLNGPANQILVASADGIGTKLRLAFTLGGEAHATIGSDIVNHCVNDILALGATP
                     LFFLDYFATGKLDPDTVEVVVGGIADACGANNLALLGGETAEMPDMYAEGEYDLAGFI
                     VGSVTEDRIIDGSGIRENDVVIGLPSTGLHTNGYSLARKTVGLSGDPEHDRQVLAAPL
                     AGTDISLGEALIAGHRSYLPEVMPLLDAGLVRGMAHITGGGLVDNLPRTLPEECIAVL
                     DPRSWSVPPILDHIVERAGMPKIERYRVLNMGIGFVLVVRPDDAVAVLQQVPDALPIG
                     RIVARRTTDESPVQGLLGERHGDD"
     gene            10490..11065
                     /locus_tag="VGR29_03500"
     CDS             10490..11065
                     /locus_tag="VGR29_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HEV2072685.1"
                     /translation="MATINVAKRRIDTKIVYYGPGQSGKTTNLVNVHARMPAELRGDL
                     RSIDTAEERTLFFDFMPVEEIEVGGWAIRFHLYTVPGQKDYVRTRRAILGGADGIVFV
                     ADASPEQSDANRESVVELHEHLVHYGRDAERLPVVLQVNKTDLPQARDSREVNDDLNE
                     ATWPMFPAMAIRGIGVAETLGAIAKAVAWTL"
     gene            11542..>12339
                     /locus_tag="VGR29_03505"
     CDS             11542..>12339
                     /locus_tag="VGR29_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2072686.1"
                     /translation="MTLIQVQRAKYALELHDVEKRFKKKRNDYSYALKGVSLSVTRGE
                     VVGILGPNGSGKSTLVRVVSTLTVPDAGVVEVFGINAVERPREVQRYINRVSVEASFF
                     KKMSSLENLLFGAKLYGVSDRESRPRIKQILDEIGFDYKRVGEPMEHLSRGMQQKIAL
                     ARALLTSPMLMLLDEPTTGLDPRSKKDVQALIRSIRKDHDSSILLCTHDMGEAEELCD
                     RIGILLNGELVAIDTADGLRQKYGTSSLEDVFMAATGTSLEEAEFAAE"
BASE COUNT         2390 a         3612 c         3920 g         2417 t
ORIGIN      
        1 agccagctgt acccttcccg cagatcgccg cgtgccgacc agtagcgcca gagcgcagcg
       61 gccaggcgaa gacctgtctc cgcctccccg caggtgagcg accattcgag agcctcacgc
      121 atgttgtcct gctcggcctc aaggcggtcc agccacttcg cttgttctcc acggatcagc
      181 tcctgctcgg cttcctcagc gagcctgaga caccaggtcg tggcttggtg gcggaccagg
      241 cccgtttccc ctgctgcgtc aaggcgttcg agaccaaatt cccggattgt ttcgagcatc
      301 cggaaacggg cctggcctgc aacggaatcc tgaatcacca ggttctgggt caccagtgaa
      361 gcaacgacat cgaaggttgg gggaactggt gccatctccc caccggcacc ggcaccggca
      421 acggcctcag ccgcgtcaag ggaaaatcca ccggcgaaaa tcgacagccg ccggaacaga
      481 gcctgttcat tctggtcaag gaggtcgtag ctccaggcga tggcatcgcg catcgtccga
      541 tggcgggccg gcagatcgcg gggcccgctg cccaacagat ccatccggga atggagccgc
      601 gccagcaggg cttccggtgg gaggatcctg ataccggcag cggctaactc gatggcaagg
      661 ggcagaccat ctacacgagc acagatttcg gccacagctg cggcgttctt ggtggtgagc
      721 tcaaagctgg ggcggacggc gcgcgatcgc tcgacgaaga gggtcaccgc gggggatgcg
      781 ttgagcgcaa atcccgcagt tcgtcgaaca ggattgacca gcgccaatgg aaccacggga
      841 aacacatgtt ccgcgcggat gttgagtgcc acgcggctcg ttgccagcac tttcagatcg
      901 ggacaactcg ccaggatgtc agccacaatg ggcgccgcct cgaccatctg ctcgaagttg
      961 tccaggatca gcagcatcct ccgcccacgg aggtgctcca tcagcgtcgc ggcaggtgct
     1021 tcgctcccgc cgccccgaac cccaagcacc tgggccaccg ttggcacgac gagggcggag
     1081 tcatggattg gggcgagatt cacaaaagcc gcaccgtctg caaacacgtc cacgagttcc
     1141 gccgccacct caagggccaa tcgcgtcttg ccaacgccgc ctgggccagt gagcgtaagc
     1201 aagcgaacaa gggattggcg aaggagttcc ccggccctgg ttacctcgtg ctcccttcca
     1261 accaagcgtg tgggaggcaa gggcaaccgc ggcccatcct ggagggggta gggcaaagcg
     1321 gagggctgga ggtccagcaa gttgagcgaa gatggccgta aagtcaagac tatgctcctg
     1381 acgcgtggct gcgggctcga tgaagagtgg caaaacgtat ggagccactg tatcggcgat
     1441 ccgcgtctat agcttcaacg cacccggcgg gacgcaggtt acgcttgggt gaatatgtgg
     1501 actggaggat aggtggcgct cgcgccttgt ctccactccg tccgcctcga cgggtgacct
     1561 cgctggacat catccagaat cccaccggtt catccttgcc caattgttgt gctcgttgcg
     1621 gcaggaggac ttctcgtacc cagccatggc ggagggctga tttaacggga gtcatcgcct
     1681 gtccccttct atatggaatt gacgggatat cacgtcgtgc ttacacttcc caagcaatgg
     1741 tacctgcggg tggccggggt gctgtgagtt ctttccccgt tatgatgttg atggatggac
     1801 cgtggtgtcg cacgcactcc ggcggagcgg acgttatgac ggcgatcacc actcttcaag
     1861 agcagtcgac gcttctggag tgtgtcgagc aacttatctc caatgacact ccggacgagg
     1921 ctcgttcgtg ccgggaagta ctggccgacc atttcgagcc cgaggtcctc gccgcgctcg
     1981 aaaacctcag cgaggccatt gagggcaacc aacacacccc gaacacgcga atggttgctc
     2041 gggccttcct ggaagcctat agcgctcatc agggcatggc tcgtaaatcc ggtgaaccct
     2101 acatcgttca tccggttgag gtcgccacca tcctcgcacg catgcatctt gatacggaca
     2161 cgcttgcggc cggtctgttg catgatgcga tcgaggacac cccggtcacc ttcaacctga
     2221 ttgcggagca ctatggggag cgggtggccc gcctggtaga aggtgccacc aagctcggca
     2281 agattccctg gacgccagat agcgacgacg gacgggagcg agtcgcccgc gagaaggaac
     2341 aacaggccga gagcctgcgc aagatgttcc tcgccatggt ggacgatatc ggggttgtgc
     2401 tcatcaagct tgccgaccgc ctgcacaaca tgcgtacgct cggggcgatg ccccgccaca
     2461 aacagattcg tatctcccag caaaccatgg aaatttatgc gcccctcgcg aaccgattgg
     2521 gcatctggca ggtcaaatcc gaactcgagg atttggcttt tcgctatctc atgcccgagc
     2581 agtatcaggc gatcgcgaac gagttggaga aacgggtcga ggattcaacc agctacctga
     2641 acgcaatcac agcgcagctg caggggttgc tcgaccaggc cgggatcaag gccgagatgg
     2701 tggggcgcaa gaagcacatc tattcgatct accggaagat gaaccgcaag gctcgacctt
     2761 tcgatgagat ctacgatgtg atcggtgtcc ggatcctcgt tgagacggtg caggactgct
     2821 acagcgtgct cggtatcgtg catggccgct ggcatccgct acccggagaa atcgacgact
     2881 acattgcgac gcccaaggag agcatgtatc aaagcctcca tactgcggtg attgggcccg
     2941 atgcgcatcc ggtagagatc cagattcgca cccgtgagat gaaccaggtc gccgagtacg
     3001 ggatcgccgc gcactggcga tacaaggaag gcggcaaggg cgatcagcgg cttgaggcca
     3061 agattgcctg gttgcgccag ctgatggact ggcgggacga tgttgcggac gccgaggaat
     3121 tcgtcgagtc gctcaagtcc gatgtcttcc aggaccaggt ctattgcttt accccggcgg
     3181 gcgacatcat cgagctgccg aacggcgcga ccccggtgga ttttgcctac cgcatccaca
     3241 cccagatcgg ccaccagtgc gttggtgcta cggtcaacgg gcaaatggtg ccgctcgact
     3301 acaagctcca gaacgcccag gtcgtgaaga tcaagaccag caagacggta aacggtcccc
     3361 gacgcgactg gttgcaggcg aactcgggct atgtgcatac agcgagcgcc cgcgaaaaga
     3421 tccgtcagtg gttccgccgc caggagcgcg acgagaacat ttcgcagggc aaggagacgc
     3481 tggaacggga gcttcgccgg ctggcccttg atatgaagcc cgacgatgtg ctggcgcgct
     3541 tcccccagta cacgaagctg gacgacttcc tcgcggcgat cgggtatgga gccgtcaccg
     3601 cgcaggccat cgccaccaag ctggacgaga caccgaagga tgtctttccc acgaccccgg
     3661 taatccgcgc cccgaccacg cctgctcgcg tcgaggtgat gggtgcaggc aacttgctca
     3721 cgatccaggc gaactgttgc cggcccgtcc cgggcgatga gatcgtgggt tacaccacac
     3781 gtgggcgggg ggtcgtctac caccgggcag actgcaccaa tatcgtcaat atctccgacc
     3841 ccgagcggct ggttccggtc tcctggggcg gccaggtcca tgagacgcat cccgttcctg
     3901 tgcggctcat tgccatggac cgggtcggcc tgctgaagga cgtcagtacg ctgttgtcgg
     3961 atgaacgggt caacatcctc tcgatgctga cgcagaccca cgacgaccgc agcgtgacgc
     4021 tgctgctgac cgtcgaggtc gagagcgtcg agcagctcag ccgcatcatg catcggctcg
     4081 agaacctgcg ggacgtcgtt gaggtacgcc gtgatacggg cagcatcacc gcgatcaacc
     4141 aggtccgggc gagttagggg tgccggggcc ggtcatcgcg attggcgagt gtctcgtcga
     4201 cctcattgcg cctggaggcg ttgacctcac cgtcgcaaat gagttgcgga ttcgtgaggg
     4261 cggtgccccg gcgaatgtgg ccgtggcgct tgcccgcctc gatgtgttgt cccaaatgcg
     4321 ggcagtgctc ggtgacgatc cctttgggga gcgccttcgg tcccggctta atcgcgaggg
     4381 tgtcgacgtg tcgaccatcc gcttcgcaga tgggatcccg actaccgtgg ccttcgcatg
     4441 gtcggacgaa cgcggcaacg ggcggtttcg gattcaccgc aatgccgacg cattgctgtc
     4501 gccggatgat gtcactcgcg agtcgctgaa tggagccgcg gcgatcgttg ttggctctgt
     4561 cgcgatgtgt gcagagccgt cgaaatcggc catcctcagc gcgcttcgcc atgcgctgga
     4621 tctcggtgta ccagtggttg ccgacctcaa catccgtccc ggtcccggtg ttccgttgga
     4681 tgaactccgt atcattgcgg cggccctcgt ctcttcagca accgtggtca agcttagcgt
     4741 ggacgactcc cgcgaacttt ggggagcaac gtcgatccgg gaggcgtcaa cggagctgga
     4801 caggttcgat cccgcgattg ccgtgattac ggatggaggg cgtggtgccg cgttgcgtgc
     4861 gtccggtcgg ctcgtggaac tcgatgtctt tccggtcgag gccatcgagc ccactggagc
     4921 gggggatgcc ttcacggctg cctttatctc ccgcatgctc gccaaggact gggcgggcgc
     4981 cgatgtggat gatctccgct tcgcgatggc ggctggggcg ctggcaacga cggagccggg
     5041 cgcgatggac ggtcttccca cccgggagcg catcgaggac ttcctgcagc tacacgcctg
     5101 atgcgcacgt gtcatcatgt cggctacgac ggatgatctt atccgcaact cgctgtggcc
     5161 gtttggcagg acaggtacat gcccagtctt catgatcccg tcgcgatcgc tcttggtccg
     5221 ctggagattc gctggtacgc aatcttcatc ctctcgggga tcttcggcgc ggtagcactc
     5281 gctcactggc tcgcgggtaa gcgcggcctc gaccaggaat tcatttttga tatcgcgcca
     5341 tgggtcgtct ttctcggcat cgccggcgcc cgcctctatt acgtgctact cgaatgggat
     5401 cggttcaagg acgatctctt tggagcgatc aacatccgtg gcggcgggct gacgattcac
     5461 ggtgcggtca tcgccggtgc gctcactgtc tggtggctct gccggagata ccgcgagcca
     5521 ctctttacct ggctggatat catcgcaccg ggcgttgccc ttgggcaggc gctcggccgg
     5581 tggggaaact gggcgaacca ggaagcgttc ggaacgccga ccgacctacc gtgggcggtg
     5641 accatcgacc cggcacggag gccctccggg tacgaggcgt tcgctacatt ccatccaacc
     5701 tttctctacg agtcgatctc caacctcgtg aatgccatgc tcctttcatg gttggtggtg
     5761 aacggcccac gcctgaagtg gtttcgggac ggggatgtga tcgggctcta cctggtcgca
     5821 tacggcgttg tccggttctt catcgagcgg attcgcaccg acagtctcta tatcggtcca
     5881 tggcccgctg cctattggct tagttttggc ttgatcgccg ccgggctcgc gatcatggtc
     5941 ttgcggcgtc gagtgccggc ggagacggga agcgtgatag tggcctccag ggaggatgcc
     6001 gcatgatgag gagggtcccc atcatcgtct gcctggtgct gatgctggcc tccggcggga
     6061 tgctcgagca gatagccgca gcccagtctg agggcgatgt gcttgacctc gcaaatgttc
     6121 ccttgacggc cgatgcactt cccgagcctg gctaccagat tctcaccggt ggatacctcg
     6181 atcgcgatgc gacggtcgaa cagattgcta gcccccggaa cgcgcaggta ggtgacgtag
     6241 aagggatgtt gatcaatacg ggcacggtac gggcctatgt gctaaatctg gtgcttcttg
     6301 aggatcgggc ccatgccggt agtgacattc tctcgctcgt ccagaccacc gtctatctct
     6361 tcgtcgacgt ggatggtgcc acggatgcct tccaggtcct cagcgattac tcctcaagcg
     6421 ccgacgcgga agaagccgaa ccggccattg tcggtgccac gacagtacgg ttgagtagcc
     6481 agagtgggga tacctaccgc agcgtggccc ggagtgagcg agctgtcatc gaggtcgtct
     6541 ccctggagac gtttgacttt gtcgatgctg acatacatcg gcgggtcgtt gcggatagct
     6601 atgatcggct ggttgacttg cagtccctct cctccggcgg gattgcgcca caggcagtgc
     6661 tgattggcga tggtgacaat gttaccgacc tcatggctgc cgatgggaca ggcgtgcatc
     6721 agctctaccg ggtacgcgag ggggaagtgc aggcggcagc gggtgagctt cggcctcctg
     6781 cgccggagga gattgcccct gggttgacgc aactgtatgt ggcgagccag ggagtcagga
     6841 tcggccaggg aaccgggttt tactcatcct ggatcggcca attcggcagc gaccccgatg
     6901 ccgcggcgtt cgtggcgggc ttgcccacct cgtcgtcaac cgcgttgcta cctgatccct
     6961 attactccct ctgggcggac gagcaggcgg tctcccaggg cgttacaggg gtctatcgcg
     7021 tctcgggggc aggggaccag ggcaccttct cgggaacgct tgagatacgt cagcacgggc
     7081 catacgtcgt cggtatcggt tggcgcacct tgggaagtgt gttgccatcc gtggatgtta
     7141 cgtcgcggct gatggacgcc cagcttacgt gtctcgaggt accggcgaca tgtgcgccct
     7201 tgccactgga ggagttgctg ccggtggatc cagcgacacc ggccattgcg gtgcctggtc
     7261 ctgacgaggc cggtagcgat gagttcggat ggtccctgcc cattgatcct cagatgtggt
     7321 cgactactga agagtttgcg gaggctgggt acgacttcat tgaattgcaa tcgggccgga
     7381 gcttggtgac ggtcgaaagc gtgatcgatc agcatggtga cccacaacag tgtgtcgtgg
     7441 acgagctacg ggacctgcag gactttgagg aacatgcggt tatcgagctg gggagcgatg
     7501 tagctggtga gcgaccggcg gggatggagg ctggtcacgc ctgggctgtg tataccgtcg
     7561 agccgctcgc cgaagaacgt gccgatcagg aatacaccat tcgcatcgac tgctacacgc
     7621 ttgtcgaagg tagtgcatcg ctcatcatga cgcatcgcgc ccctcggtac gaatgggaag
     7681 gtgaacgatt gaagggggag gcactacggg atgcgctcca actgccatca gggaagcgga
     7741 gaggtggtat aattacgcca cagcatccct acaactggag gtggatcatg atcgataaaa
     7801 tttggattgg tctcgcggcc taaatagtgc ttgttattaa acaattattt tgtggcgggt
     7861 gcctccagac acgcgcacca ttcactgtgc gcattgcatc tggataccgt gcgcatcggg
     7921 ctccttccac tgcccgaaag gaactgtcat aagcttcgac attgaacttc atacacaatc
     7981 cacagccgga gaacctgaac gggctctcct cgtcgcggtc gaaacgccat cacaggcgtg
     8041 gaccgcgtcc gattccctgg aagaactcgc gagtcttgcc gagacggttg acgttgaggt
     8101 cgtaggctcg gtgacgcaga agctcggcca tccccatcct gcaacgtatg tgggaaaggg
     8161 caagttggaa gagatcaagg ggctccagga ggatctcgat tacaatctgc tgcttgtcga
     8221 tggcgagctc gcgccggcgc agatacgcaa tcttgagcag gcgctcgaga tgaaggtcat
     8281 cgaccggaca gcattgatcc tcgacgtctt cgcacggcgt gcacagacgc acgagggtcg
     8341 tctccaggtc gagcttgccc agatgcaata ccgtctgcca cgcctcacac ggatgtggtc
     8401 tcacctttca cgccaaggtg tgggcggtgt cggtctgcgc ggtcccggtg agacgcaact
     8461 tgaggcggac cgccgtgaag cgcagaagcg aatcgccttc ctcaagggtc agatggagca
     8521 ggtgcaccgg catcgggagc tctatcgtgc ccgtcgcaag gatacgccgg tctccatcgt
     8581 ctcgcttgtt ggttacacca acgccggcaa gagtacgctg atgaaccaga tcaccggagc
     8641 tggtgtgctg gctgaggaca agctctttgc gacgcttgat ccgacgacac ggcgtatcac
     8701 gctgccgagc gggcgtgagg tcctgctcac cgacacggtg ggatttatca ataatctgcc
     8761 gacgcagctt atcgcggcct tccgcgcgac gctcgaggaa atcagcgagg cgtcgatgtt
     8821 aattcacgtg gtggatgtga cgcatcagaa cgccacggag caggtccaga cggtgaagga
     8881 gacgcttgag gaactcggcg tcgacgatcg cccggtgatc tacgccatga acaaggtcga
     8941 ccgtctcacc aaaaagcagc gagcggactt gcgggaagta gcgccgaaga tgggggttcc
     9001 atccgatgcg atcgccatct ccgcacagac cggtctaaac atcgagggtt tgctcaatgc
     9061 tgtcgagaca gttcttgagc aggaagaagg gttcgttccg gtaacggcgc gcatcccatt
     9121 caaccggtcg gagctggtcg agcgcttcca cacgaatggc cgcgtggatg agcgggactt
     9181 cgatgagaac ggcacaaccc tcacaggctg gctgccggag cgggagctcg ggcagttcat
     9241 cccgtacgtg acgctcgacg gaacgcaccc gaacgggcat gatgagagcg tcctgtctga
     9301 ccactccggc gaggtaacgg cgaattctgt aaactagggc gaccacacga gatcgcctca
     9361 ggcggcgggg atcacgcaag tggtccccgt tctgtttttc gtcgtctcat tccacgaagg
     9421 gtgcccatgg atcggccaac ctccatcagc tacaaggatg caggcgtcga tatcgacgcc
     9481 aagatgcagg caatcgatcg cctgaaggcg cgcgcccaat cgacgctggg tgcatccgct
     9541 ggtccaatcg ggcactttgg cggtacctgg cggctcaatg gacctgcgaa ccagatcctt
     9601 gtcgccagtg cggacgggat cggcacgaag ctccggctcg cctttacgct cggtggtgag
     9661 gctcacgcca cgatcggctc ggacatcgtg aaccactgtg tgaacgacat ccttgcgctt
     9721 ggggcaaccc cgctcttctt cctcgattac ttcgctaccg gcaagttgga cccggacact
     9781 gttgaggtcg ttgtcggcgg gatcgctgat gcctgcgggg cgaataacct ggccctgctc
     9841 ggcggcgaga cggccgagat gcccgatatg tatgccgagg gagagtacga cctcgcaggg
     9901 ttcatcgttg gctcggtgac ggaggaccgt attatcgatg gctccgggat ccgcgagaac
     9961 gatgtggtca tcggattgcc gagtaccggg ctccatacca atggctacag cctcgcccgg
    10021 aagaccgtag gtttgtccgg tgatcccgag catgaccgcc aggtcctggc tgctcctctc
    10081 gctgggacag acatctctct tggtgaggca cttatcgccg gacatcgctc ctacctgcct
    10141 gaggtgatgc cgttgctgga tgccggtctg gtccgtggga tggcacacat taccggtggc
    10201 gggcttgtgg acaacctccc gcgcacgctc cccgaggagt gcatcgcggt gcttgatcca
    10261 cgatcctgga gcgtacctcc aatcctggat cacatcgtcg agcgagcggg gatgccgaaa
    10321 atcgagcgct accgggtcct caacatgggc atcggattcg tgctcgtcgt gcgcccggac
    10381 gacgctgtcg cggtattgca gcaggttcca gatgccttac caatcggcag gatcgttgca
    10441 cgccggacga cagacgaatc gccggtgcag ggtctgctcg gggagcgtca tggcgacgat
    10501 taatgtcgcc aaacgccgca tcgacacgaa gatcgtttac tacggcccgg gccagagcgg
    10561 aaagaccacc aacctcgtga acgtccatgc gcgcatgccg gcagagcttc ggggcgatct
    10621 gcgctcgatc gataccgcag aggagcggac gcttttcttc gatttcatgc ccgtagagga
    10681 gatcgaggtc gggggctggg caattcgctt tcacctctat acggtgcctg gtcaaaagga
    10741 ttacgtgcgc acacggcggg cgatcctggg cggagcagat gggatcgtgt tcgtggcgga
    10801 tgcatcgccg gagcagtccg atgccaatcg ggagagcgtg gtcgagttgc acgagcatct
    10861 ggtgcattac ggcagggatg cggaacgatt gccggtcgtg ctgcaggtca acaagactga
    10921 tttgccccag gcgcgagaca gtcgtgaagt caatgacgac ctgaacgagg cgacctggcc
    10981 gatgtttccg gcgatggcga tccggggcat cggtgtcgct gaaaccctcg gtgcaatcgc
    11041 aaaggcagtg gcctggacgt tgtgagtgga ggacggctcc ccactcgtaa tcgccggatt
    11101 tcgcgggaca attcaggcag ccctcgccca aggctgcctg tgcatcgatt ggtcagccta
    11161 ttgacatctg gccaacgcgg ctctaggatg cgaagggttc actcacatgt gcgtgaacgg
    11221 ttcacgtttc gtccgaggta agcggcacca tggttttcct ggctctccat atccccgcat
    11281 tccccaacac gggtctcccg ggggaggagt gtgccatgct cgcagacgtg cctgactgac
    11341 ctacatgcac aactgacgag catgcatctc ccttcgacgg gcccagtcga agggtttttt
    11401 gtgccccttt ttcttgcgcg tcactggatc gcatccatcg ttatggaggt ttggagaaat
    11461 cacatgatga agcgaaggtt ctatcgtcca taacgggaac tcgatacagg gattaccaga
    11521 tatgaaagaa agtggatggc catgacattg atacaggtcc agcgagcgaa atacgcgctg
    11581 gaactccatg acgtcgagaa gcgattcaag aagaagcgca acgactactc gtacgcactc
    11641 aagggcgttt cgctctccgt gacgcggggc gaggtagtcg gcattctggg gccgaacggc
    11701 tctggcaaga gcacgctggt gcgagttgtc tctacgctca ctgttccgga cgccggcgtc
    11761 gtggaggtgt tcggaatcaa cgcggtcgag cgtccacgag aggtgcaacg ctacatcaac
    11821 cgcgtcagcg ttgaggcgag cttcttcaag aagatgtcct cgctggagaa cttgctcttt
    11881 ggcgcgaagc tctacggtgt cagcgatcgc gagagccgac cgcgcatcaa gcagatcctt
    11941 gacgagatcg gattcgacta caagcgcgtg ggcgagccga tggagcacct gtcgcgaggt
    12001 atgcagcaaa agatcgcgct ggcgcgagcg ttgctcacca gcccgatgct gatgctgctt
    12061 gacgagccga caaccggcct ggatccgcgg tccaagaagg acgtgcaggc gttgatccgc
    12121 tcaatccgca aggatcacga ctcatcgatc ctgctctgca cgcacgacat gggtgaggcg
    12181 gaggagctct gcgaccggat cggaatcctg ctgaacggcg agctggtcgc gatcgatacc
    12241 gctgacggct tgcggcaaaa gtacgggacc tcctccctgg aggatgtctt catggcggct
    12301 accggaacaa gtttggagga ggcggagttc gccgcagaa
//
LOCUS       DASYWM010000085        14410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_171878, whole genome shotgun sequence.
ACCESSION   DASYWM010000085 DASYWM010000000
VERSION     DASYWM010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14410
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171878"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1719
                     /locus_tag="VGR29_03510"
     CDS             310..1719
                     /locus_tag="VGR29_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEV2072687.1"
                     /translation="MTDSAANQTTAYSTGPLDPDVGKLFKAYDVRGIVPQGLNPDIAY
                     RIGRALVSFLGTDQVVVGRDMRVSGPRLQAALMDGIRDQGADVIDIGLVSTDTLYFAV
                     GKYDYPAGVMITASHNPAAYNGFKICREGARALSMETGIADIRDLVASGAFPETASAT
                     RGRHEERDVLEAYAEHALSMVEKSRIKPLKIAVDAGNGMAGKLAPPVFEQLATEIVPL
                     YFDLDGTFPNHEANPIEPENIRDLQRTVVEQKCDLGVAFDGDADRMFLIDENGEFVGG
                     DMTTAMVSLQMLKKHPGSTIVYNLICSRTVPEIITEHGGRAIRSRVGHSFIKALMREE
                     DAIFGGEHSGHFYFRDNWYADSGLIAMLSVLELISDENQTLSEILEPIDSRFRSGEIN
                     SEVDDTDAVISKIEKHFAAEGAEIDHLDGLTVGFDDWWFNLRASNTQPLLRLNVEAED
                     QRTLEEKENKVLRLIRGNE"
     gene            1716..2777
                     /locus_tag="VGR29_03515"
     CDS             1716..2777
                     /locus_tag="VGR29_03515"
                     /inference="COORDINATES: protein motif:HMM:NF040503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HEV2072688.1"
                     /translation="MSPALGYLTARPAIIEDVPRITEIYNQGITDRAATFETQPRKED
                     AVRGWIDSRYPVVVVERDGEVIAWAGASSYRPRDCYAGIAEFSVYVDRAVRGKGAGRA
                     AMQALIDAARNAGLEKLVSRVFVENTASRRLLHSIGFREVGVYERHAKLDGVWRDVVI
                     VELLLQDHEGEPHGALWQADLSTLQLWPELMPGREVFIEKRNEFGKDGPIYPGMIIES
                     STPEPWIEVRATWTLYRVQVGGLRFEIGDELREFFSPRHPFNAFAIYSPEGELRGWYG
                     NVTRTARCEPRNDTFLVAWPDMMLDLAMLPDGTSVDLDDDELIESGFPEREPDISRQM
                     LDARETLRRLLSDGFFPIR"
     gene            complement(2833..3039)
                     /locus_tag="VGR29_03520"
     CDS             complement(2833..3039)
                     /locus_tag="VGR29_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072689.1"
                     /translation="MSKHPEPVGIRVHPEPTPEEMTAISAAVAALASRPYTDREESPS
                     GVDSRERWREAARREILRSSERDS"
     gene            complement(3036..4601)
                     /locus_tag="VGR29_03525"
     CDS             complement(3036..4601)
                     /locus_tag="VGR29_03525"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEV2072690.1"
                     /translation="MTDVTADATSTKAQHLAEFREQMAKQHAEAREKQCGRGRRSALD
                     RVHALLDEDSFLELDAFVRHRATQFGMGKAKPFGDGVVTGFGTIDGRKVAIFSQDFTI
                     FGGSLGEAYAEKMVKLMDLAEQYGCPIIGINDAAGARIQEGVEGLAGYGDVFFRNVRA
                     SGVVPQISIIAGPCAGGAVYSPAMTDFIFMVDGIGQMFITGPDVIKTVTGETVTLEDL
                     GGSEAHSTRSGVAHFRATDEDDLNEQVRYLLSFLPSNNLDDPPVFEMDDDPDRESPAL
                     DEIVPEHSARPYDMHEILREVLDEGQFFEVQSDYARNLIVGFGRLDGQSVGIVANQPA
                     VLAGTLDIGASVKGARFVRFCDAFNIPLVTFVDVPGFLPGTDQEYNGIIRHGSKLLYA
                     FCEATVPKVTVITRKAYGGAYVVMCSKHSGADFNVAWPTAEIAVMGPDAAVGLVFRRE
                     LETAEDPAARRSELIKDYEDLFASPYQAASRGYIDDVIEPRITRPWVIKALDLARAKR
                     VAAPKRKHGNIPL"
     gene            4812..6947
                     /gene="pta"
                     /locus_tag="VGR29_03530"
     CDS             4812..6947
                     /gene="pta"
                     /locus_tag="VGR29_03530"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV2072691.1"
                     /translation="MPRNIYITSAEGHTGKSTVALGLLTLLKRSARNIGVYRPVSRSR
                     NEPDHVLNFLLSQLDRAVLAAECIGVTYEDVHTDPEGALTQIITRYKAMEHRHDVVII
                     LGSDYTDVSSPTELRYNARIAANLGAPVLLVLGGRSHDETASVQSHVLAGQGRTASEM
                     YQTAEVALTELQEEHAQLVAVIANRVDPDILEEVTAAVKQALHPSGAIPVWSLPDDPV
                     LVAPTLAELSDMLDGHQLKGDPELLHREVLAYVISGMSMENILVRLTEGAAVVVAGDR
                     SEVLLAILMAHSADTFPQLSGIILNGGFSISPTIERLISGIGTDLPIVSTDLNTFETA
                     LRITRARGQLTANSQRKIDVALSLFDEHVDEEALISSLEMDRASVVTPLMFEYQIIDR
                     ARSDRKRIVLPEGDDDRILRAASTLLQRHAAELTILGDKDDITTRAARLDLDLSSARI
                     VSPHDPALVERFAEEYARLRAHKGMTIAKARHIITDVSYFGTMMVHMGLADGMVSGAA
                     HTTAHTIKPSFEIIKTSPDVSVVSSVFFMCLEDRVLVYGDCAVNPNPTAEQLADIAIS
                     SARTAARFGVEPRIAMLSYSTGESGAGDDVERVRTATRLVRERRPDLLVEGPIQYDAA
                     TDASVAAAKMPDSSVAGRATVFIFPDLNTGNNTYKAVQRSAGAVAIGPVLQGLRKPIN
                     DLSRGALVKDIVNTVAITVIQAQGEGEPA"
     gene            6944..8131
                     /locus_tag="VGR29_03535"
     CDS             6944..8131
                     /locus_tag="VGR29_03535"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV2072692.1"
                     /translation="MSVVLVVNSGSSSIKYQIIEMESEEVLGKGLIERIGTGSGAATH
                     TGPEGEQRSERPILDHVAGFQAMTDACDAYGPILDEVPIVAVGHRVVQGGSRFVMPTV
                     IDSAAIETIEEISPLAPLHNPPNLLGITAARRAFPDLPHVAIFDTAFHQTIPAEAYTY
                     AIDANIASRYQVRKYGFHGTSHKYVSELAATYLQLPPGDVNTIVLHIGNGVSACAVRG
                     GVSVDTSMGMTPLEGLVMGTRSGDIDPGILFHLHREAEMTIDQLDDMLNRHSGLLGMM
                     GSSDMRDVLASVQHGEEHAQLALGVYCYRLRSYVGAYYAQLGKVDAIVFTAGIGENAP
                     EVRAQTLAGLEHMGIRLDVARNTSPDRDIRSISSDDSAVAVLVIPTNEELEIARQTLN
                     AIT"
     gene            complement(8282..10246)
                     /gene="dnaK"
                     /locus_tag="VGR29_03540"
     CDS             complement(8282..10246)
                     /gene="dnaK"
                     /locus_tag="VGR29_03540"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV2072693.1"
                     /translation="MGRTIGIDLGTTNSVMATIEGGEPVVIPNAEGERLTPSVVAVTN
                     SGERLVGRFARRQAVTNPENTVSSVKRFVGRKFNDPAVERDKSLVPYALQAAPNGDVQ
                     VKMGDKWYSPQEVSAMILQKLKTDAEAYLGETVDKAVITVPAYFDNAQRDATKDAGRI
                     AGLEVLRIINEPTASALAYGLDKKGDEKVAVYDLGGGTYDISILDLSEGVFQVLATNG
                     DTHLGGDDFDQVIIDWLADEFKKQEGIDLRKDRMALQRLKEAAEKAKIELSSTQQTDV
                     NLPFITADASGPKHLNVPLTRSKLEQLVAPLINRTKAPMESALKDAGLSKSEVNEALL
                     VGGQTRMPAVQKTVKDFFGKEPHKGINPDEVVAIGAAIQGGVLGGDVKDILLLDVTPL
                     TLSIETLGGVATPLIERNTTIPARKSQSFTTASDSQPQVEINVLQGERPMAADNKSLG
                     RFILDGIPPAPRGVPKIEVTFDIDANGILNVAAKDSATGKEQKITITGSSGLSEEEVQ
                     RMVTEAEQHASEDQKRREAIETRNNAESLMFQAEKLVEENGDKIPDDLKSDLEAKIAD
                     VKDVLENDRENIDRIRPAHDALMESLQKAGSAMYEAAGAAGADMGGAEGGFPGGFTGD
                     AGAGASSQTDDESTVEGEFREVNDDESKAS"
     gene            complement(10321..10911)
                     /locus_tag="VGR29_03545"
     CDS             complement(10321..10911)
                     /locus_tag="VGR29_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV2072694.1"
                     /translation="MTHAEQDRGQTIAEETTEEITTEEVATDTGDGERDELSQALAER
                     DDYLEQLQRSRAEFINFRKRSEQERLKLGELFTANTLRQFLPVIDDFDRAMGAVPEKD
                     QGSGWLAGISMIHQKLQGILERAGVEPIDALNKPFDPSIHEAVASEPGTSGGTVVEVY
                     QKGYRLGDTLLRAAMVKTGDAATDTDTGENSEHFDA"
     gene            complement(10975..12027)
                     /gene="hrcA"
                     /locus_tag="VGR29_03550"
     CDS             complement(10975..12027)
                     /gene="hrcA"
                     /locus_tag="VGR29_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV2072695.1"
                     /translation="MDTLQPEREEQRLTERQRMVLRLLVREYVVSGRPVGSKTLAEQF
                     PMSVSSATIRNELAALEHVGFVQQPHTSGGRIPTDQGYRYYVHHLMGSPELSSGDQIM
                     IRHQFRQVEAQLDSWVDFAAEVLAETAGNVSIVTAPRSMVSRLRHFEMISLQPRMALL
                     ILVTQESAVRQMMLHLPEDADQATLSRLTDVMVPMVRGLGAEDVESQIVGATELPAYV
                     LEQIAIGLRGMGSVEHTQIRHSGLENILGQPEFAGAAGHNVLELLRGSALLGALLPQM
                     SVGHEVQVFIGDENPADALRHFGVVMATYGIDGEVTGILGVLGPTRMSYWRSISSVRY
                     MARLMSDLMEDLYSSS"
     gene            complement(12446..13162)
                     /locus_tag="VGR29_03555"
     CDS             complement(12446..13162)
                     /locus_tag="VGR29_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072696.1"
                     /translation="MELVSLHPFDPDIATRYVAALKGEREPDPAWRDWWSPVLPDALA
                     RTAAGREDAAQSVSLGLAWALSAEHPVFVREGFGLTNWEARIDRGVGMLMRPPSRLFI
                     DAGLERRTAQVMPIRLELEQGMMGGAYIPSRLMGNMAELMDARLERMAKRLVDAEYDP
                     FSMLGLMHQAVAYACEHDLGLYEAQDAVGPAVPRMRLVEAAEKKRMDGALRERIEAAI
                     TPEKKPGIISRLMGKKKPGE"
     gene            13379..14044
                     /locus_tag="VGR29_03560"
     CDS             13379..14044
                     /locus_tag="VGR29_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2072697.1"
                     /translation="MPQTARATTSLRLSIELSTKLDEKPRERCPTVHFMVSGSTGNRE
                     ISTLRTDVEQPRIDAEDHAFSMVDARDVLSRVSDEEAWLAELHRVISPGGHLSFTVPA
                     AGVLAWFDARNIYRYATDILKRGDAPDDTLPTGWNRHYHEDEVRHLLEVTGFEPRAME
                     RVGIGIAEIPQLAGLLVGNFLLGKRDTELKLHPLRERMESYDQTLTVPGIGTSLYVLA
                     ARI"
     gene            complement(14132..>14410)
                     /locus_tag="VGR29_03565"
     CDS             complement(14132..>14410)
                     /locus_tag="VGR29_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072698.1"
                     /translation="LAANHADERKPDQTDSQFYYTTRKRGFGPFKRQMWSMPEGTRET
                     ILDELQPKFELIMQKLRVAGNGHLVDQHIKRVDVDVPVPEVVQQVAQA"
BASE COUNT         2837 a         4578 c         4097 g         2898 t
ORIGIN      
        1 agtgtgacgc cgtctacccg gatgtccctc gaggggaagg cgttgcgcgc ctgtcccttc
       61 gcctccgtca tggctgaccc tttcaacccg cgtacgccca gtgtgtccaa taagcaatta
      121 cagagtggga taccttcccg atttgatgag catcgaatgg agcaacaatc ggggaaatca
      181 cggcaagatg caaggattca ttcagcctcg tcacttcgga tatgccggca ccattatagt
      241 tagcggtaga acgaagagac gcgccggaac agtccgctcg cggctacctg caccattggg
      301 aggatagata tgaccgactc cgcggccaat cagaccaccg cctattcaac aggcccgctc
      361 gaccccgacg tcggcaaact cttcaaggcc tatgacgtcc gcggcatcgt gccgcaggga
      421 ctcaacccgg atattgccta ccggatcggt cgggcgcttg tcagcttcct gggcaccgat
      481 caggtggttg tcgggcgcga catgcgtgtg tcaggaccca ggctccaggc cgcgttgatg
      541 gacggcatcc gcgaccaggg cgcggacgtc atcgatatcg gcctcgtctc caccgatacc
      601 ctctacttcg ccgtgggcaa gtatgactac cctgccgggg tgatgattac cgcgtcacac
      661 aacccggctg cctacaacgg cttcaagatc tgccgggagg gagcacgggc cctctcgatg
      721 gagaccggaa tcgctgatat tcgtgacctc gtcgcgagcg gagccttccc cgagaccgcc
      781 tccgccacac gcggtcgaca cgaagagcgc gatgtccttg aggcgtatgc cgaacacgcc
      841 ttgagcatgg tggagaagag ccggatcaag ccactcaaga tcgcggtgga tgccggcaac
      901 ggcatggcag gcaagctggc gcctcccgtc ttcgagcaac tcgcaaccga gatcgttccg
      961 ctctacttcg acctcgatgg cacattccca aaccacgagg ccaacccgat cgagcccgag
     1021 aacatccgcg atctccagcg caccgtggtg gagcagaaat gcgatctggg tgtcgccttc
     1081 gatggggacg cggaccgcat gttcctgatc gatgagaacg gagaattcgt cggcggcgac
     1141 atgacaaccg ccatggtctc gctgcaaatg ctgaagaagc atccgggatc caccattgtc
     1201 tataacctca tctgctcgcg cacggttccg gagatcatca ccgagcacgg cggtcgggcg
     1261 atccgttccc gggtgggcca ttctttcatc aaggcgctga tgcgcgagga agatgcgatc
     1321 tttggcggcg agcactcggg tcacttctac ttccgggata actggtacgc ggacagcggc
     1381 ctgatcgcga tgctctccgt gctggagctc atctccgatg aaaaccagac actctccgaa
     1441 attctcgaac ccatcgacag ccgattccgt tcgggcgaaa tcaactccga ggtcgatgac
     1501 actgacgccg tgatctcgaa gatcgagaag catttcgccg cagagggggc ggagatcgat
     1561 cacctggacg ggctcaccgt cggcttcgac gactggtggt tcaacctgcg ggcatccaac
     1621 acgcaacccc ttctgcggct gaacgttgag gccgaagacc agcgcacgct tgaggaaaag
     1681 gagaacaagg tgctcaggct catccgaggt aacgagtgag tccggccctt ggctacctga
     1741 ccgcacgtcc cgcgattata gaggatgtgc cccgaattac tgagatctac aaccagggca
     1801 tcaccgatcg cgcggcgaca ttcgagaccc agccgcgcaa ggaagacgcg gttcgcggtt
     1861 ggattgattc ccgctacccg gtcgtcgtgg tggagcgcga tggcgaggtt atcgcctggg
     1921 cgggggcctc ctcctaccgt ccacgggatt gctacgcggg gatcgccgaa ttttctgtct
     1981 atgtcgaccg agccgtccga ggcaaaggag ccggacgggc tgccatgcag gccctgatcg
     2041 atgccgcccg gaatgccggt ctcgaaaagc tcgtctcacg tgtctttgtc gaaaataccg
     2101 ccagccgtcg actcctacat tctatcgggt ttcgcgaggt cggcgtctac gaacgacacg
     2161 cgaaactcga tggtgtctgg cgggacgtgg tcatcgtcga gctgctcttg caggatcacg
     2221 aaggtgaacc acacggcgcc ctctggcaag cagacctgtc gacgctccaa ctctggccag
     2281 agctgatgcc tggccgcgaa gtctttattg agaagcgcaa cgaattcggc aaggatggac
     2341 cgatttaccc cgggatgatc attgagtcgt cgactcctga gccgtggatc gaggtcaggg
     2401 caacgtggac gctctaccgg gttcaagtag gaggtttgag attcgagatc ggcgatgagc
     2461 tgcgggagtt tttctcgccg cgacatcctt tcaacgcatt tgccatctac tcgcccgaag
     2521 gggaacttcg tggttggtat ggcaatgtga ccaggaccgc gcggtgtgaa cctcggaatg
     2581 acaccttcct ggtcgcgtgg cccgatatga tgctcgatct cgccatgctg cccgatggta
     2641 catccgttga tctcgacgac gacgagttga tcgaatccgg gttccctgaa cgcgagcccg
     2701 acatcagccg gcaaatgctt gatgcgcgtg agacccttcg aaggctactc tccgacgggt
     2761 tctttcccat ccgctagcct ccgacctcag gtccggactg catcctcgac gattcggatc
     2821 gcagatgcga gtttaagaat cccgctcgct cgaccgtaga atctcgcggc gagcagcttc
     2881 tctccatcgc tcccggctat ccacccctga aggggactcc tccctgtcgg tataaggacg
     2941 tgacgccagt gcggcgacag cggcactgat cgccgtcatc tcctccggag tcggttccgg
     3001 atgcacccgg attcccacag gttcaggatg cttgctcata gaggaatatt cccgtgcttg
     3061 cgcttcggtg ccgcgactcg tttcgcccgt gccaaatcca gtgccttgat aacccatggc
     3121 ctcgtgattc gcggctcgat cacatcatca atatacccac gggaggccgc ctggtagggg
     3181 ctggcgaaaa gatcctcata gtccttgatc aactctgacc gccgtgcggc cgggtcctcc
     3241 gccgtctcca gctctcgccg aaagacgagt cccacggcgg catctgggcc catcaccgcg
     3301 atctccgccg tcggccaggc gacattgaaa tcggcaccgc tgtgcttcga gcacatcacc
     3361 acataggctc ccccgtaggc cttgcgcgtg atgacggtta ccttgggcac ggttgcctca
     3421 cagaaggcat agagcaattt ggagccatgc cggatgatgc cgttgtactc ctggtcggtt
     3481 cccgggagga atccgggcac atcgacgaag gtgaccagcg ggatattgaa cgcgtcgcag
     3541 aatcgcacga accgcgcacc cttgacgctg gcaccgatgt ccagcgtgcc agcgagtaca
     3601 gctggttggt tggccacgat cccgacgctc tggccgtcca gccgaccaaa gccaacaatc
     3661 aggttacgag cgtagtcgga ctgaacctcg aagaactgcc cctcatcgag cacctctcgc
     3721 aggatctcgt gcatgtcata cggccgtgcg ctgtgctccg ggacaatctc gtccaacgcg
     3781 ggcgactccc gatccgggtc gtcatccatc tcgaagactg gcggatcgtc caggttgttg
     3841 gacggcaaga aggagagcag atagcgaacc tgctcgttga ggtcgtcctc gtcggtcgca
     3901 cgaaaatggg cgacgccgct ccttgtgctg tgggcctccg agccaccaag atcttccagc
     3961 gtgaccgtct ctccggtcac cgtcttgatc acgtccgggc cggtgatgaa catctgcccg
     4021 atgccatcga ccatgaagat aaaatccgtc attgccgggg agtacacggc gccacctgcg
     4081 caggggccgg cgatgatcga aatttggggg acaacaccag aggctcggac attccggaag
     4141 aagacatcac cgtagccggc gaggccctcg acgccctcct ggatccgcgc accggccgca
     4201 tcgttgatac cgatgatagg gcagccgtac tgttcggcca gatccatcag cttcaccatc
     4261 ttctccgcat acgcttcgcc gagggagcca ccgaagatgg taaagtcttg tgagaagatg
     4321 gcgaccttgc gaccatcgat ggtaccgaaa cccgtcacca ccccgtcgcc gaacggcttt
     4381 gccttgccca tgccgaattg agtggcgcgg tgccgaacaa aggcatcaag ctccaggaaa
     4441 gagtcctcgt caagaagcgc gtgaacgcga tcaagcgcac tccgcctgcc gcgcccgcac
     4501 tgcttttccc gcgcctcggc atgctgcttc gccatttgct cgcggaactc cgcgagatgc
     4561 tgtgccttcg tgctcgttgc gtccgccgtt acatcagtca tccgatggct tcccttccct
     4621 gctccctcca gtcctgctgc gtcgcattgt agcgggaggc gaagcatctg cgccctgacc
     4681 tgcccacgtc tgagatcctg ctggcccgaa tccatcaggc gcaacaagat acggttccgt
     4741 agacttgtca ctagaggacc gggcacccca tggcctgatg cggcgcgatg ggaggctcag
     4801 gagggaccag catgccacgt aacatctaca tcacctctgc ggaaggtcat accgggaaat
     4861 cgacggttgc gctcgggctg ctgaccctgc tcaagcgatc cgccaggaat atcggcgtgt
     4921 atcgtcccgt ctcccgctcc cgcaatgaac cggatcacgt cctcaatttt ctgcttagtc
     4981 aattggatcg tgctgttctc gcggccgaat gcatcggtgt aacctacgag gatgtccata
     5041 ccgacccgga gggggcactc acccagatca tcacgcgtta caaggcgatg gaacatcgcc
     5101 acgatgtagt tatcattctc ggttcggatt acaccgatgt ctcctcgcca acagaacttc
     5161 gatacaacgc ccgaatcgct gcaaaccttg gtgcgcctgt cctgcttgtg ctcggcggtc
     5221 gcagccacga tgagacggct tcagtgcaaa gccacgtgct ggctggccag ggccgaactg
     5281 cgtcagagat gtatcagacc gcggaggttg cgctcaccga actccaggaa gagcacgccc
     5341 agctcgttgc cgtgatcgcc aaccgcgtcg atcccgacat actggaagaa gtcaccgctg
     5401 ccgtgaagca agccctccac ccgtccggtg cgatcccggt ctggtcgctt ccagacgacc
     5461 cggtccttgt tgcgcccacg cttgctgaac tctcagatat gctcgacggc caccagttga
     5521 agggcgatcc tgaactgctg catcgcgaag tgctggccta cgtaatttcc gggatgtcca
     5581 tggagaatat cctggttcgc ctgacggaag gtgcagccgt agtcgttgcg ggagaccgct
     5641 cggaggtgct gctcgcgatc ctcatggccc acagcgcgga tacctttccc caactctccg
     5701 gcatcatcct caatggcggc ttctcgatct ccccgacgat cgagcgactt atctccggca
     5761 tcggcaccga tctcccgatt gtcagcaccg acctgaatac gtttgagact gccctgcgaa
     5821 ttacacgagc ccgcggacaa ctaacagcga actcccagcg caagatcgat gtcgcgctat
     5881 ccctcttcga tgagcacgta gacgaggaag cgctgatctc ctcactcgaa atggatcgtg
     5941 cgagcgtggt gacaccactg atgttcgaat accagatcat agatcgtgcc cggagcgacc
     6001 ggaagcggat cgtcctgccg gagggggatg atgaccgcat tctccgcgcg gcaagcacac
     6061 tgcttcagcg ccacgccgcc gaactcacca tactcggcga caaggacgac atcacgaccc
     6121 gcgcggcccg cctcgacctc gacctctcct cggcgcgaat cgtcagccct cacgacccgg
     6181 ctctcgtcga acgattcgcg gaggagtacg ccaggctacg tgctcacaag ggcatgacaa
     6241 tagcgaaagc ccgccacatc atcacggacg tctcctactt cggcacaatg atggtgcaca
     6301 tgggcctcgc cgacgggatg gtgtccggcg ccgcgcacac gaccgcccat acgatcaagc
     6361 cctcgttcga gatcatcaag acgtcgccgg atgtctcagt cgtctccagt gtcttcttca
     6421 tgtgtcttga ggaccgggtg ctggtctacg gcgactgcgc agtaaacccg aacccgacgg
     6481 cagagcagct ggccgatatc gccatatcct ccgccagaac cgcagcccgg ttcggagttg
     6541 agccacgaat cgcgatgctc tcatattcca ccggggaatc gggcgcgggc gacgatgtcg
     6601 agcgagtgcg gactgcaacc cggttggtcc gggagcgccg gcccgatctc cttgttgagg
     6661 gaccgatcca gtacgatgcc gctactgatg cttcagtcgc agcagcgaag atgccagatt
     6721 ccagcgtcgc cgggcgggcc acagtgttta tctttcccga ccttaatacc gggaacaaca
     6781 cgtacaaggc ggtgcagcgg agcgcagggg ccgttgcaat cggccccgtg ctacagggcc
     6841 tccgcaagcc gatcaacgat ctgtcgcgcg gtgccctggt caaggacatt gtcaacaccg
     6901 tcgcaattac cgtgatccag gcacaaggag aaggcgagcc agcatgagtg tcgttctggt
     6961 agtgaactcg ggctcctcat ccatcaagta ccagattatt gagatggaat cggaagaggt
     7021 cctgggcaag ggtctgatcg aacggatcgg caccggaagc ggtgccgcca cccataccgg
     7081 tcccgagggc gagcaaaggt cagaacgccc gattctcgat catgtggcgg gcttccaggc
     7141 gatgacagat gcctgcgacg cgtatggtcc aatactggac gaggtaccca tcgtagcggt
     7201 cggacatcgc gtagtacaag gtggttcccg attcgttatg ccaacggtca tcgatagtgc
     7261 tgctatcgag acgattgagg agatcagccc gctcgcccca ctgcacaacc cgccgaacct
     7321 actggggatt accgccgccc gccgggcatt tcccgatctc ccccatgttg cgatcttcga
     7381 caccgccttt caccaaacca tccccgcaga ggcatacact tacgccatcg acgcgaacat
     7441 tgcgagccgg taccaggtcc gcaagtacgg attccatggc acctcccaca agtatgtctc
     7501 cgagcttgca gccacctatc tccagttgcc accgggggac gtcaacacca tcgtgctcca
     7561 tattggcaat ggtgtttccg cgtgcgctgt ccgaggtggt gtctctgttg atacgtcgat
     7621 ggggatgaca ccgttggagg gcctggtgat ggggactcgt agcggggata tcgacccggg
     7681 gatcctcttt caccttcacc gcgaggccga gatgacgatc gaccagcttg atgacatgct
     7741 caaccgccac agtggcctgc ttggcatgat gggaagctcg gacatgcgag atgtgctcgc
     7801 atccgtccag catggcgagg aacatgccca acttgccctc ggcgtctact gctaccgctt
     7861 gagaagctac gtaggagcgt attacgcgca gcttggcaaa gtagacgcca tcgtattcac
     7921 cgcggggatc ggtgagaatg cccccgaggt acgagcccaa acacttgccg gcctggagca
     7981 catgggcatc cggctcgatg tcgctcgtaa cacgtcaccg gatcgtgaca tccgctccat
     8041 ctcctccgac gattctgccg ttgccgtcct ggtcattccg acaaacgaag aactggagat
     8101 cgcgcgccag acgctgaatg cgattacgta aggggcggac gtagcaccga agtgaggggc
     8161 taccgtcctt cgttctctgt ctttactctg gcatggtgaa tcaaacgcca gtaccgaaac
     8221 aggcgggtgg attactccac ccgcctgttt cgcgtatctc aactgtgacc agagcctgga
     8281 atcaggatgc cttgctctcg tcgtcgttta cctcccggaa ctcgccctcg acggtcgact
     8341 cgtcgtccgt ctgggagcta gcacccgcgc ccgcatcacc tgtgaagccg cccgggaatc
     8401 cgccttcggc gccacccatg tcggcaccag ccgcaccagc ggcctcgtac atcgctgaac
     8461 cagccttctg gagcgattcc atcaacgcat catgcgccgg ccggatccgg tcgatgttct
     8521 cgcggtcgtt ctcgagcacg tccttgacat cggcgatctt cgcctcgaga tccgacttca
     8581 ggtcgtccgg gatcttgtcg ccgttttcct caaccagctt ctcggcctgg aacatcaagc
     8641 tctcggcatt attccgggtc tcgatcgcct cacgccgctt ctggtcctcg ctagcgtgct
     8701 gctcggcttc cgtgaccatc cgctggacct cttcctccga gagaccagag gaacccgtga
     8761 tggtgatctt ctgctccttg cccgtggcgg agtccttcgc ggccacgttg aggatgccgt
     8821 tcgcgtcgat gtcgaaggtc acctcgatct tcggcacacc gcgcggtgcc ggcggaatgc
     8881 cgtccagaat gaagcggccg aggctcttgt tgtcggcggc cattgggcgc tcgccctgga
     8941 gaacgttaat ctccacctgc ggctggctgt cagacgccgt cgtgaagctc tggctcttgc
     9001 gagccgggat cgtcgtattg cgctcgatca gcggcgtagc cacaccgccg agcgtctcga
     9061 tcgagagcgt cagcggggta acatcgagca ggagaatatc cttgacatca ccgccgagca
     9121 cgccgccctg gatcgccgcg ccgattgcga ccacctcgtc cgggttgatg cccttgtgtg
     9181 gctccttgcc aaagaagtcc ttgactgtct tctggacggc cggcattcgg gtctggccac
     9241 ctacgagcag ggcctcattg acctcgctct tcgacaggcc agcgtccttg agtgctgatt
     9301 ccatcggcgc ctttgtccga ttgatcagcg gcgccaccag ttgctccagc ttcgagcgag
     9361 tcagcggcac attgaggtgc ttcgggccgc tcgcgtcggc cgtgatgaac ggcaagttga
     9421 cgtcggtctg ctgcgtgctg gagagttcga tcttcgcctt ctcggccgct tccttcaggc
     9481 gctggagggc catacggtcc ttgcggagat cgatgccctc ctgcttcttg aactcgtccg
     9541 cgagccagtc aatgatcacc tgatcgaagt catcaccacc gaggtgcgta tcgccattcg
     9601 tggcaagcac ctggaagaca ccctcggaga gatccaggat tgagatgtcg taggtaccac
     9661 cgccgaggtc gtagaccgcg accttctcat cacccttctt gtcgagacca taggccagcg
     9721 cggatgccgt cggctcgttg atgatgcgca gaacctccag accagcgatc cggccggcgt
     9781 ccttggtggc atcccgctgc gcgttgtcga aataggccgg aaccgtgatg actgccttat
     9841 cgactgtctc accaagatag gcctcggcgt cagtcttcag cttttgcagg atcatcgcgg
     9901 agacttcctg cgggctgtac cacttgtcac ccatcttcac ctgcacgtcg ccattcggcg
     9961 cggcctgcag ggcataggga accagcgact tgtcacgctc caccgccggg tcgttgaact
    10021 tgcgtccaac aaagcgcttg acggaggaaa cggtgttctc cgggttcgtc actgcctggc
    10081 ggcgggcaaa gcggccgacg aggcgctccc cgctgttggt caccgcgacc accgacggcg
    10141 tcaggcgctc gccctccgcg ttcgggatca cgaccggctc accaccttcg atggtagcca
    10201 tgaccgagtt cgttgttccg aggtcgatac cgattgttcg tcccatagtg tttgttctcc
    10261 tttaatatct gcggcttccc gcgattcttt gcccaagtgt tgctcggggc tggcactcac
    10321 ctatgcgtca aaatgctcgc tgttttcgcc agtatccgta tcagtggcgg cgtctccggt
    10381 cttcaccatg gccgcccgca agagagtgtc gccgagcctg tatccctttt gatacacctc
    10441 aactaccgtg cctccactgg tgcccggttc ggatgcgacc gcttcatgaa tggatgggtc
    10501 aaagggcttg ttcagtgcgt cgattggctc aacgccggca cgctcaagga ttccctgcag
    10561 cttctggtgg atcatcgaga tcccggccag ccaaccagac ccctgatcct tctctggcac
    10621 agcccccatc gcccgatcga aatcatcgat tacaggcaag aattgcctca gggtgttggc
    10681 agtgaagagc tcaccaagct tcaatcgctc ctgctcgctc cgcttgcgga agttgatgaa
    10741 ctccgcccgg ctacgctgga gctgctcgag atagtcgtca cgttcagcga gggcctgcga
    10801 cagttcgtcc cgctctccgt ctccagtatc cgtcgccact tcctcggtcg tgatctcctc
    10861 tgtcgtctct tccgcgatag tctgtccgcg gtcctgttca gcatgtgtca ctcaaacacc
    10921 tccttacgac gaagcagatg ctccggcgca gatatgtcca ttgtttcgta ttcgttagct
    10981 agatgaataa aggtcttcca tcaaatcgct catcaaccgc gccatgtagc gtaccgaaga
    11041 gatcgatctc cagtacgaca tccgggttgg ccccagtact cctaggatcc cggtgacctc
    11101 gccatcgata ccgtaggtgg ccatgacgac gccgaaatgt cgcaaggcgt cggccgggtt
    11161 ctcatcgccg atgaaaacct gaacctcatg acccaccgac atctgcggga gcaaggctcc
    11221 caagagcgca ctaccacgga gcaactccag gacgttatgc cccgcggccc cggcgaactc
    11281 cggctgcccg agaatattct cgagacctga atgccggatc tgggtgtgct caaccgatcc
    11341 catgcccctc aggccgatgg cgatctgctc caggacgtat gcgggcaatt ccgtagcgcc
    11401 tacaatctgc gactcgacgt cttccgctcc aagtccccgg accatcggaa ccatgacatc
    11461 tgtcaaccgg ctgagcgttg cctggtctgc atcctcaggc aggtggagca tcatctgcct
    11521 gacagcactc tcttgcgtga ccaggatcag caaggccatc cgtggctgca gggagatcat
    11581 ttcgaaatgg cgcagccgtg agaccatcga cctcggagcc gtgacgatgg aaacgttgcc
    11641 cgcggtctct gccaggacct ccgcagcgaa atcgacccag gagtcaagct gtgcctcaac
    11701 ctgccggaac tggtggcgga tcatgatctg gtcgccggag gagagttccg ggctccccat
    11761 cagatggtgt acgtaatagc gatacccctg atcggtcgga atccgcccac cggaggtgtg
    11821 aggctgctgg acaaagccga catgctccaa cgccgccagt tcattgcgga tcgtggcaga
    11881 agagacagac atcgggaact gctccgcgag cgttttcgac cccaccgggc gaccgctcac
    11941 cacgtattcc ctgacgagca atcgcaaaac catgcgctgc cgttccgtca gtctttgctc
    12001 ttcgcgctcc ggttgcagcg tatccacccc gtctccgctc ctcctctgtt agcactctct
    12061 gccgacgagt gctaacacgg aaatggtagc aattttatac aatccttgtc aactttgaag
    12121 gcggaatttg ctccggttaa aagatgtacc tcagggccct tgccggcgca gagaggcccc
    12181 taggaattgg cgattggctg tggtgcctgg gatatcgcgg caagcgagat gaagaggaat
    12241 gtgcctcaga atcagggcgg gatgacggaa gtgctatcag gatgctcggg cctctggagc
    12301 cccttaggtg aaggggtacc acttgagact cacggttgta atgccgcgcc agtctcggtg
    12361 cggcactcct tccaatccgc gacgaaagat ggtctcagga gaatgcggaa tcagagagac
    12421 attcaggttc aatcctctcc tggatctatt ctcccggctt cttcttgccc atcagccgcg
    12481 agatgatccc cggctttttc tccggagtga tcgccgcctc gatgcgctcg cggagtgctc
    12541 cgtccatccg cttcttctcg gctgcctcca cgaggcgcat acggggtacc gccggcccta
    12601 ccgcatcctg cgcctcgtac aacccgagat cgtgctcgca ggcatatgca acagcctgat
    12661 gcatcagtcc gagcatcgag aacgggtcgt actctgcgtc aaccaggcgc ttggccatcc
    12721 gctccaggcg cgcatccatc agctccgcca tgttgcccat gagacgtgat gggatgtagg
    12781 cacctcccat catcccctgc tcaagctcga gacgaatagg catcacctgc gcggttcggc
    12841 gctccagtcc cgcatcaatg aagagacggg agggcgggcg catcaacatg ccaacgccgc
    12901 ggtcgatccg cgcttcccag ttcgtgagac caaatccctc ccgaacgaag acgggatgtt
    12961 ccgctgacag ggcccacgca agaccaagcg atactgactg agcagcatcc tcgcgtcccg
    13021 cggcagttcg agccagcgcg tcgggcagta ctggactcca ccaatcacgc cacgccggat
    13081 ccggctcacg ttctccctta agggcggcaa catagcgtgt ggcgatatcg ggatcaaagg
    13141 ggtgtagtga tacaagctcc acgtgactca ccttttcact gtgtccagag gaatgcgata
    13201 ccaaagatga tgccacgaag ccgttctatc gatggtctca ccaacaaaaa tcaggggtgc
    13261 cgcgcatcca cgcttatctg cgccgtgcga gccgaccgtc tttgtataga ctaaagcgca
    13321 tagcaacatc aacgacactg cgccggatca cttgagtgcg gaaaggacca cgatgatcat
    13381 gccccagacc gctcgagcca ccacctcgct ccggctctcg atcgagctca gcaccaagct
    13441 ggacgaaaaa ccccgggagc gctgtccgac ggttcacttc atggtcagcg gcagcaccgg
    13501 aaatcgtgag atcagtactc tgcgaacgga tgttgagcag ccccgcattg acgccgagga
    13561 ccacgccttt tccatggtcg acgcccggga tgtgctttcg cgcgtgagcg atgaagaggc
    13621 atggcttgca gaacttcatc gcgtgatttc accgggcggc cacttgagtt tcacagtccc
    13681 ggcagccggc gtacttgcct ggttcgacgc gcggaatatt taccgctatg ccactgacat
    13741 cctcaagcgg ggggatgcac cggacgatac cttgcccaca ggctggaatc ggcactatca
    13801 cgaggatgag gttcgccacc tccttgaggt aaccggcttc gagccacgtg ccatggagcg
    13861 ggttggcatc ggcatcgcgg agatccccca attggccggt ttgctggttg gcaacttcct
    13921 actcggcaag cgggacaccg aactgaagtt gcaccccttg cgggagagaa tggaatcgta
    13981 cgaccagacg ctcactgttc cagggatcgg cacgtctctt tacgtcttgg ccgcaagaat
    14041 ctagggtgcc gaagagcgca cctgcggcat gggggcgaac acagggtctg ccctcatgcc
    14101 tgagctgtca gggagcctgc cgaaagttgc cctatgcctg tgctacctgc tggaccacct
    14161 ccggcaccgg aacatcaacg tccacgcgct taatatgctg gtccacgaga tgcccgttcc
    14221 cggcgaccct cagcttctgc atgatgagct caaacttcgg ctgcaactcg tcaagaatag
    14281 tctcacgcgt tccttccggc atgctccaca tctgccgctt gaacgggcca aaccctcgct
    14341 tgcgagtggt gtagtagaac tgggagtcgg tctggtccgg cttgcgctca tctgcgtgat
    14401 tcgccgcaag
//
LOCUS       DASYWM010000086         4454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_434529, whole genome shotgun sequence.
ACCESSION   DASYWM010000086 DASYWM010000000
VERSION     DASYWM010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434529"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VGR29_03570"
     CDS             complement(<1..460)
                     /locus_tag="VGR29_03570"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV2072699.1"
                     /translation="MEAREAIKSALKYAPRFLEFQQPHMPFVGAQVAVRHDGELLLNH
                     AAGFADLETKTPLTVDHLFRIASHSKTFTGVACMQLVEQGKLRLDDEAMTHVFELAES
                     PMGGVTVRELLGHGSGMTRDGEDSTFWSLDRTFPDKEELLDTIRQHGKVLD"
     gene            complement(540..1907)
                     /locus_tag="VGR29_03575"
     CDS             complement(540..1907)
                     /locus_tag="VGR29_03575"
                     /inference="COORDINATES: protein motif:HMM:NF005034.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2072700.1"
                     /translation="MGSTTDLRNVYSYIDEHKDEYVARLQRLLRQPSIAAQSLGMDEM
                     ATLVEQDLAELGTVPRRIDTRGGFPVVYAEFSGANNRTLSFYNHYDVQPADPLDEWES
                     DPWSGEIRDGRIWARGVSDNKGNIAARLAAIDAWQKVRGPLPLNVKFIIEGEEEIGSP
                     HLQNFADDHPELCQADACIWEFGGRDVDGRPQIHLGLKGICYVELRVRGARTDWHSAS
                     ATSVPNPAWRMVWALATLKGEDDRILIPGFYDNVVAPTQAELEALANLPDTEQERLDD
                     LGIDRFINDLTGQELHLKDYFQPTCTISGFLSGYTKKGQKTVLPSQAMAKLDMRLVAN
                     QDPHAIYELLRTHLDANGFTDIESELIGAGYPARTSLEAPIAKVVAETYEDIYGQVPA
                     IYPTSAGSGPWYQLCDAFGIDACTAGVGHGRSQAHAPNENIFVEDFVLGIKHIAAIMD
                     RFARR"
     gene            complement(1916..3148)
                     /locus_tag="VGR29_03580"
     CDS             complement(1916..3148)
                     /locus_tag="VGR29_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEV2072701.1"
                     /translation="MGQSPDHAKPRTLQELEEAVLARIDQDDVVAFHRGLVQIPSVNP
                     PGDCRDAIAYVEKPLREAGFEIQIVQDLEAMPNLLASTGPASGKVLCVNAHVDVVPTG
                     EREAWSHDPFGAEIENGRIYGRGAGDDKASVTAQVLAALAVKRSGVPLAGRLVVNEVS
                     DEETAGVHGVQVVVNQRAVEPDWVIVGEQTLNRVCVGEKGSAPTTITVQGRTAHGALP
                     WEGANAIEGMAEVIVALRRELWPELMQRTHRFFHPSSGSVNMFAGGVKANVVPDKAEI
                     YVDRRLVPGENPQDALDEIRVIAERALQNVPGTSVEVSLSEWAGAATEAAVDDPLVQA
                     MLGANERLGLSAEPAGFSMATDGRYFARAGYPTIIYGPGDPGLAHKPDEWVGIDEIMD
                     AVRAYAVTMVRLIGDYEK"
     gene            3253..3768
                     /locus_tag="VGR29_03585"
     CDS             3253..3768
                     /locus_tag="VGR29_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEV2072702.1"
                     /translation="MSDSAKKVAIVLANNYEDVEATSPIEALENAGAEVTIVGIEKGE
                     ITGKKGDTQTASKTFADVSPDDFDMLLIPGGGSPENLRINDAAVQFTRDFVQSGKPVA
                     SICHGPQLLISARVVSGRKLTSVNKIRDDIVNAGGLYVDEELTEDGNLITSRVPGDLP
                     AFNEAITKALG"
     gene            3882..>4454
                     /locus_tag="VGR29_03590"
     CDS             3882..>4454
                     /locus_tag="VGR29_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2072703.1"
                     /translation="MSTEASAIEEPTGNQHLDRLQSAKGFIFDMDGVLYRGKMVLPGV
                     EDLFNALTLRQIPFLLATNNSMATPAAYVQRMASMGVTVEEADIQTSATATRDFLVDD
                     EETPNDAVILTVGMPALGEQLSMGTSFRTLGENEPASDANVVVAGLDLTFTYDKMKRA
                     CEAIDHGARFIATNADDRLPTETGYQPGAGA"
BASE COUNT          887 a         1402 c         1258 g          907 t
ORIGIN      
        1 ggtccaacac cttcccatgc tgccggatcg tgtcgagcaa ctcctccttg tcggggaacg
       61 ttcgatcgag cgaccagaag gtgctgtcct ctccgtctct cgtcatcccg gagccatgcc
      121 ccagcaactc cctcaccgtc accccgccca tcggcgactc tgcgagctca aacacgtgtg
      181 tcatggcctc gtcgtccagc cgcagcttcc cctgttccac cagctgcatg caggccacac
      241 cggtgaaggt cttggagtga gatgcgatcc ggaacaggtg atccaccgtc aagggcgtct
      301 tggtttccag atccgcgaaa ccggctgcat ggttgaggag aagctctccg tcatgccgca
      361 ctgccacctg cgcgcccacg aagggcatat gaggctgttg gaactccaga aaccgcggag
      421 catacttgag cgcgctcttg attgcttccc tagcttccaa cttcctcgac ccttccactt
      481 gatgtggtca tcatcgactc tggaccctga aagatccggg tcgcttcaac ctgtcagatc
      541 tatcttctcg caaagcggtc catgatcgcg gcgatgtgct tgataccaag gacaaaatcc
      601 tcgacgaaga tgttctcgtt cggtgcatgc gcctgggatc gtccatggcc aacgcccgcg
      661 gtacacgcgt ctatgccgaa cgcatcacac agctggtacc agggccccga gccggcgctc
      721 gttgggtaga tggccggaac ttggccgtag atgtcctcgt acgtctcagc gacaaccttt
      781 gctatcggcg cctccaggct tgtccttgcc ggatagcctg cgccgatcag ctccgactca
      841 atgtcggtga agccgttcgc gtccagatgc gtccgaagca gctcgtaaat cgcatgcggg
      901 tcctggttcg ccaccagacg catgtcgagc ttcgccattg cctggctggg cagcaccgtc
      961 ttctggccct tcttggtgta cccgctcaga aatcccgaga tcgtgcacgt cggctggaag
     1021 taatccttga ggtgcaactc ctggcccgtc aggtcgttga tgaaacgatc gatgcccaaa
     1081 tcgtccagcc gctcctgctc ggtatccggg agattcgcca atgcttcgag ttcagcctgc
     1141 gtcggtgcga caacattgtc atagaaaccg ggaatcagga ttcgatcgtc ctctcccttt
     1201 aatgtggcga gcgcccacac catgcgccat gcgggatttg ggaccgacgt cgccgaagcg
     1261 gaatgccagt ccgttctagc gcctctgacg cgcagctcga cgtagcagat gcccttgagg
     1321 ccgagatgta tctgtggccg gccgtcaaca tctcgaccgc caaactccca gatgcacgcg
     1381 tcggcctggc aaagctcagg atgatcgtcg gcgaagttct ggagatgcgg agagccaatc
     1441 tcctcttcgc cttcgatgat gaacttcacg ttcaacggca gggggccacg gaccttttgc
     1501 caggcatcga tggctgcgag tcgagcggcg atgttgccct tgttgtccga tacgccacga
     1561 gcccagatac gaccgtcgcg gatctctccc gaccagggat cgctttccca ttcgtccagc
     1621 ggatcagcgg gctgcacgtc atagtggttg taaaagctca gcgtgcggtt attcgctccg
     1681 gaaaactcgg cataaacaac cgggaatcct cccctggtgt cgatccttcg cggcacggtc
     1741 cccagttcag ccagatcctg ctcgaccagg gtagccatct cgtccatgcc gaggctctgg
     1801 gcggcgatgc ttggctgccg gagcaagcgc tggagccggg ctacgtattc gtccttgtgc
     1861 tcgtcaatgt agctgtacac atttcgcaga tcagttgttg atcccataga gtcccctact
     1921 tctcgtagtc accgatcagt cgaaccattg tgacggcata ggcacggaca gcgtccatga
     1981 tctcgtcaat gccgacccac tcgtcgggct tgtgagcgag acccggatcg ccaggtccgt
     2041 agataatcgt gggatacccc gcgcgcgcga agtagcgtcc atccgtggcc atgctgaagc
     2101 cagccggctc cgctgagagt ccaagtcgct cattcgcccc gagcattgcc tgcaccagcg
     2161 gatcatcgac agccgcctcc gtcgccgcgc cggcccattc cgagagggac acttccaccg
     2221 atgtgccggg aacgttttgg agtgcccgct ccgcgatcac ccggatctca tccagcgcat
     2281 cctgcggatt ttcccctggt accaggcgtc gatcgacata gatctcggcc ttgtccggca
     2341 caacattagc cttcacgccg ccggcaaaca tgttgaccga gccggacgag gggtgaaaga
     2401 acctatgggt gcgttgcatc agttccggcc acagctcgcg tcggagggcc acgatgacct
     2461 ccgccatgcc ttcaatcgcg ttggcgcctt cccaaggcag ggcgccatgt gcggtccgcc
     2521 cttgaacagt gattgtggtt ggtgcagaac ccttctcacc gacacacact cggttcagcg
     2581 tctgctcacc aacgatgacc cagtccggct caactgccct ctggttgact acgacttgta
     2641 ctccgtggac tccggcggtc tcttcgtccg aaacttcatt gacaacgaga cgtcccgcca
     2701 gaggaacacc ggatcgcttt acggcgagcg cggccaacac ctgtgcggtc acgctcgcct
     2761 tgtcgtcccc ggctcccctc ccatagattc gcccattctc gatctcggca ccaaaggggt
     2821 catgggacca tgcctcgcgc tcgcctgttg gcacgacatc gacatgcgca ttgacacaaa
     2881 gcaccttgcc actagcaggt ccggtgctgg caaggagatt cggcatggct tcgagatcct
     2941 gcacgatctg gatctcgaag ccagcttcac gtaggggctt ctccacatag gcgattgcat
     3001 cgcgacaatc cccgggaggg ttgaccgacg ggatctgcac gagaccccgg tgaaaggcga
     3061 caacgtcgtc ttggtcaatc cgcgcgagga cggcttcctc tagctcttgg agtgtgcggg
     3121 gtttcgcgtg atcggggctt tgtcccatgg tgtaccctct ctggtgttga tcttgcgtca
     3181 gccttgacaa gaacggatga ccggccgacg gaataactgc cgccgaccgc tacacaaggg
     3241 agtttccaac agatgagcga ttccgcaaag aaggtggcca tagttctggc caacaactac
     3301 gaggatgtag aggctacatc gccaatcgag gctcttgaga atgcgggtgc cgaagtgacg
     3361 atcgtgggga tcgaaaaggg cgagatcacg ggcaagaagg gcgacacgca gacggcgtcg
     3421 aagacgtttg ccgacgtctc tccggacgac tttgacatgc tgctgattcc cggcggcggc
     3481 tctcccgaga atctccgcat taacgatgcg gcggtgcagt tcacccgtga cttcgtgcag
     3541 tcgggcaagc cggttgcttc catctgtcac ggtccacagc tgttgatctc cgccagggta
     3601 gtctcgggca ggaaattgac ctccgtcaac aagatccgag acgacattgt caacgctggg
     3661 ggtctctacg tcgacgagga gctcaccgag gacggcaacc tcatcacctc ccgcgtacca
     3721 ggcgatctgc ccgccttcaa cgaggcgatt acaaaggccc tcggatagtt ctgaacaaca
     3781 gtccccggga tgcgcgagta tcccgggttt tctgtgccca gcaagcgtct ggaatggtac
     3841 gatccttcca ttaagacgtc accgcttcag aaaggcagag gatgagcaca gaggcttccg
     3901 ccatagaaga accgaccggg aaccagcatc tcgaccggct gcagtccgcc aagggtttta
     3961 tcttcgacat ggacggtgtg ctctatcgag gcaagatggt gctccctggc gtagaggacc
     4021 tcttcaacgc cctgacacta cggcaaatcc ctttcctcct cgcgacaaac aattccatgg
     4081 ctacgccggc ggcttacgtc cagcgcatgg caagcatggg cgtcaccgtc gaagaagcag
     4141 acatccagac atcagcgaca gcaacgcgcg actttctggt tgacgatgag gaaacaccga
     4201 acgatgcggt cattctcacc gttggcatgc ccgctctcgg tgagcaactg tcgatgggga
     4261 catcgttccg gactctgggc gagaatgaac ccgcaagcga tgcgaacgtt gtagtagccg
     4321 gcctcgatct caccttcacc tacgacaaga tgaagcgagc ctgcgaggcg atcgatcacg
     4381 gtgcgcggtt cattgccacg aacgctgatg atcgcctgcc gacggaaacg ggataccagc
     4441 caggggccgg agcg
//
LOCUS       DASYWM010000087         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_584928, whole genome shotgun sequence.
ACCESSION   DASYWM010000087 DASYWM010000000
VERSION     DASYWM010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584928"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VGR29_03595"
     CDS             <1..386
                     /locus_tag="VGR29_03595"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2072704.1"
                     /translation="TPLMGLLFFVAVMIGFITKALIVFSVSLLTFWTLNGVGLMWAQQ
                     AVIAILSGTLVPIALMPGWLRVLAEVLPMRGIVSTPLTFYLGKADGREIVFLLLLQVF
                     WLAVMWWFANWAWQKAFNAVDIQGG"
     gene            634..1458
                     /locus_tag="VGR29_03600"
     CDS             634..1458
                     /locus_tag="VGR29_03600"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2072705.1"
                     /translation="MNLSLPTPRSVRRNLRLYFILQFVQLRAAVEYRADFWIGILGAM
                     LQQAAGLVFISALFSQIPEVSGWTVWDIAILYGLAMIPKGLTEFFCDGPWMLRSKVNT
                     GEFDRVLVRPISPALQSATAIVSIHGFGQILLGVVVLWLGMSRSEMQLAWWTFPFLLI
                     TLLASSIMIGAINFIINMIGFWEPSAQSALPTMVALMIDFAKFPLDIYNMVIKGLVTI
                     VVPYAFISYFPALLLLGKATPWRWLGLATPLATAVVVAFAAWLWSKALNRYQGVGH"
     gene            complement(1646..2992)
                     /locus_tag="VGR29_03605"
     CDS             complement(1646..2992)
                     /locus_tag="VGR29_03605"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEV2072706.1"
                     /translation="MSDRVTFPDDFLWGAATASYQIEGAVHEGGRGESIWDRFSHTPG
                     KTANGETGDVAADHYHRWQGDIGIMQELGLKAYRFSTAWPRILPEGRGAVNRAGLDFY
                     DRLVEGLLEAGIDPWVTLYHWDLPQALQDRGGWANRDTIDAFAEYTDAITRRVGDRVK
                     NWITINEPWVVSIMGHYWGVMAPGHKDLKEALQVAHSLLVGHGRAVDVIRSNVPDGRV
                     GITLNLSHVYPDSDRNEDLEAAHTMDGFSNRWFLDPVMKGGYPDDMLALYGDAAPEVE
                     PGDFDLTARSTNFLGINSYNPQYAKADPASSMGMTSADREGERTAMGWIVEPQGFQDL
                     LVRVQNDYAPPAIYVTENGAAFNDPAPVDGRVADPQRTSYIRRHLLAAHNAIEQGAKL
                     EGYFVWSLLDNYEWAHGYSQRFGITRVDYQTQERTIKDSGRFYADVIASNSVPVEG"
     gene            complement(2989..>3282)
                     /locus_tag="VGR29_03610"
     CDS             complement(2989..>3282)
                     /locus_tag="VGR29_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072707.1"
                     /translation="FFELDAMWAAVGGYEPDEVIKSHADRIRLVHLKDASREDPRTDV
                     PFGEGVLDWGAILSASRNAGVEYYITEQDRPNPDDVLGDVRTSLVNAEGMAES"
BASE COUNT          608 a         1040 c          942 g          692 t
ORIGIN      
        1 caacgccgct gatgggcctg ctctttttcg tcgccgttat gatcggcttc atcaccaagg
       61 ccctcatcgt cttctccgtc tcgctgctta ccttctggac gctcaatggc gtcgggctga
      121 tgtgggccca gcaagcagtg atcgcgatcc tctccggcac gcttgtgcca atcgcgctga
      181 tgccgggctg gctgcgcgtg ctcgcggaag tgctgccgat gcgcggcatc gtctccacgc
      241 ctctgacctt ctatctcggc aaggcggacg gacgggagat cgtcttcctg ctcctcctgc
      301 aggtcttctg gctggcggtc atgtggtggt tcgcgaattg ggcgtggcaa aaggcattca
      361 acgccgtcga catccaggga gggtaacgcg acgatgacga agctcggcag cctcgcaagt
      421 gaccgtcatt ccgggcggag caaagatttt cgccgcgaag ccggtgcccc ggtacatttg
      481 gtgcccgggc aagccattat agtcagagta atccgcatcg cctcaagcag gattcgcatg
      541 accgagtttg caaggaggaa aaggctcgct accgcccggc caaggcagct gaattctgga
      601 tgacgttgat tctcaccctt tgagtgttga gccatgaact tgtcactccc gactccgcgc
      661 agcgtccgtc gcaatctgcg actctatttc attctgcagt tcgtccaatt acgcgcggca
      721 gttgagtatc gcgctgactt ctggatcggc atactgggcg cgatgctcca gcaagccgcg
      781 ggtctcgtct tcatctccgc ccttttctcc caaattccgg aggttagcgg atggaccgtc
      841 tgggacatcg cgatcctcta tggcctggcg atgattccga aagggctgac ggaattcttc
      901 tgcgatggac cgtggatgct taggtcaaag gtgaatactg gcgaattcga tcgcgtgctc
      961 gtgcgtccga tctccccggc acttcaatcg gcgaccgcca tcgtctccat ccatggcttc
     1021 gggcagattc tgctcggggt cgttgtgctc tggctgggaa tgagccgatc ggagatgcag
     1081 cttgcctggt ggacgtttcc gttcctgctg ataacgcttc ttgcgagcag catcatgatc
     1141 ggggcgatca acttcatcat caacatgatc ggcttttggg agccatcggc acaaagtgct
     1201 ctcccaacaa tggtcgcgct gatgatcgac ttcgcgaagt ttccgctgga catctacaac
     1261 atggtcatca aggggctggt gactatagtg gtcccatatg cgtttatcag ctacttcccg
     1321 gccctgctgc tgcttggaaa ggcaactcca tggcgctggc tcggcctggc aacacccctg
     1381 gcgacggccg tcgtcgtggc attcgccgcc tggctatgga gcaaggcgct gaatcgctat
     1441 cagggcgtgg ggcactagcg atcaatcgac tggcaccaag cgcacggagc gctcacctgt
     1501 tcccaatgca accagcgaca gagggaaagc cccgaaccat tcccggcccg gggctttccc
     1561 tctatcaata tctctgcgct ggctggctgt tcagcgaaag tgacaagggt gtcttaccta
     1621 tctcatgaga agcggagccg gcgtcttatc cctctacggg cacagaattc gaggcgatga
     1681 catcggcgta gaagcgaccg ctgtccttga ttgtccgttc ctgcgtttgg tagtccaccc
     1741 gggtgatgcc gaaccgctgg gaatacccgt gagcccactc atagttgtcc agcaatgacc
     1801 aaacgaagta gccctccaac ttcgcgccct gctcgatggc gttatgcgca gcaagcaaat
     1861 gccgccggat gtaggatgtc cgctgcgggt cggcgacccg tccgtccacc ggtgcaggat
     1921 cgttgaacgc cgcgccattc tcggtgacgt agatcgcggg cggcgcataa tcgttctgca
     1981 cgcgtaccag caggtcctgg aagccctgcg gctccacgat ccagcccatc gccgtccgct
     2041 caccctcgcg gtccgccgac gtcatgccca tagacgaagc ggggtcggcc ttcgcatact
     2101 gcggattgta ggagttgatc ccaagaaaat tggttgatcg cgcagtcagg tcgaaatccc
     2161 ctggctccac ctcgggggca gcatctccat atagcgccag catgtcgtcg gggtacccac
     2221 ccttcatcac cgggtcgagg aaccagcgat tggagaagcc atccatcgta tgggcggcct
     2281 ccaggtcctc gttcctgtcg gaatccgggt agacgtgtga gagattgagg gtaatgccga
     2341 ccctcccgtc cggtacattg ctccggatca catcgacagc acggccatgc cccaccagca
     2401 atgaatgcgc aacctgcagg gcttccttca gatccttgtg ccccggagcc ataacgcccc
     2461 agtagtgccc catgatggag acgacccagg gctcgttgat ggtgatccag ttctttaccc
     2521 ggtcacccac gcgccgggtg attgcatccg tatactcggc aaaggcatcg atcgtgtctc
     2581 ggttggccca gccgccccga tcctgcaatg cctgcggaag gtcccagtgg tagagcgtga
     2641 cccagggatc gatccctgcc tcaagcagcc cctcgaccag ccggtcgtag aaatcgagtc
     2701 ccgcccgatt gaccgcgcca cgcccctcgg gcaggatccg tggccatgcg gtggagaagc
     2761 gatacgcctt cagccccagt tcctgcatga tgccgatatc gccctgccac cggtgataat
     2821 ggtccgcggc gacatctccg gtctcgccgt tcgcggtctt gcctggtgtg tggctgaacc
     2881 ggtcccagat cgattcgcca cgcccaccct cgtgcacggc cccctcgatc tggtaggacg
     2941 ccgttgcggc accccacagg aaatcatcag ggaaggtgac tcgatcgctc acgactcggc
     3001 catcccttcg gcattgacca gagacgtgcg cacatcgccc aggacatcgt ccgggttcgg
     3061 cctgtcctgc tcagtgatgt agtactcgac gccagcattc cgggacgcgg agagaatcgc
     3121 gccccagtca agcacaccct caccaaaggg gacatccgtc cgtggatcct ccctggatgc
     3181 atccttaagg tggaccagcc ggatgcgatc ggcatggctc ttgatcactt cgtccggctc
     3241 atacccccca acggcggccc acatcgcgtc gagttcgaag aa
//
LOCUS       DASYWM010000088         4704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_96877, whole genome shotgun sequence.
ACCESSION   DASYWM010000088 DASYWM010000000
VERSION     DASYWM010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96877"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..695
                     /locus_tag="VGR29_03615"
     CDS             30..695
                     /locus_tag="VGR29_03615"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV2072708.1"
                     /translation="MEHAGQSAGALHEKPTIAHQGAVIGEDGRGRCPWAVTHPLNLAY
                     HDTEWGMPVHGEHALFERIMLEAFQSGLSWLTILRKRPAFRAAFAGFDPDIIARFTED
                     DIVRLMNDAGIVRNRAKILAACTNAQAVVALREQGGLDELIWSHKPEVTPAPETLADL
                     LTQTSESRALAKGLKSHGFVFVGPTTAHALMEAIGLVDTHLIGCHRRGCSGVFPTVKE
                     GGP"
     gene            692..1216
                     /locus_tag="VGR29_03620"
     CDS             692..1216
                     /locus_tag="VGR29_03620"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2072709.1"
                     /translation="MTTAEERVEKLIVKLDAAWNAFQDSYAGLSDDELLEPGVTGEWS
                     VRDLIAHIAWWEEESLKHLPLVLEGGRPQRYSVKYGGIDAFNALMTGQKQDLLLDEIF
                     HESGETHRRLIEYVRSIPPEAFVGDTRARRRLRLDTYGHYPIHTRDIREWRERRGKAR
                     GENTDPKWKRQTLL"
     gene            complement(1376..1915)
                     /locus_tag="VGR29_03625"
     CDS             complement(1376..1915)
                     /locus_tag="VGR29_03625"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2072710.1"
                     /translation="MASAPVPQPDERRRVRASDTPRVVTSVNAPSVDLGALGVRFLVW
                     AEESGGGFSLVEHSIPPRTLAAPLHLHTEEDEYSYVLKGRVGALLGDNVIHAGPGDLV
                     FKPRGQWHTFWNAGDMPCRILEIISPGGFERMFAEMGADPEAFTGEHAAAVDERYGLE
                     VDYESIGRLCEEYDLVFPE"
     gene            2525..>4704
                     /locus_tag="VGR29_03630"
     CDS             2525..>4704
                     /locus_tag="VGR29_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV2072711.1"
                     /translation="MTRSTHPVTIESCPTPMWTVRLDGTIEYVNPAWGAYSGISGEGA
                     VDESWVAAVHPEDVASFRDRFQSAAQKGEAYQVEYRFRRAAGTFRWHLANISPLPDAD
                     GVIRRWLGIAIDIDDRRREEADLRASEAHFRGTVESAGDIVYTLALDGIVTAVNPAIR
                     RVLGYTPEEVQGRSIEETLLPTDQLDVAREALQRKLAGEERGPYELDVLAKDGRRVTL
                     EINSRLTVAGGQPGAIQGIARDVTMRTQTQEGLARLAAIVEDSDDAILSRTLDGTITS
                     WNLGAERLYGYTAEEAVGGHVTRLVPPGRDEEVDQLTDRLARGERVPHFETVRLRKDG
                     TLIDVSISLSPLTDPDGRLIGISTIARDITARKQSEARLRLLAEAGRHLASDPNVDAQ
                     LTAVARLAVPTLADWCVVDLLDNGSDLRRVAVAHVDPVKLAMAEELQLRYPPDPAAPR
                     GPHEVMRTGEPEFMSDIPDELLVAAAHDAEHLRLLREVGLRSYLTVPLHARGRVLGTL
                     TFIAAESGHAYGPDDLPICEALAELAALAIDNAQLYRNAQEAEIRYRTVFTGVADAIL
                     VADTKHRYLEANPAATELLGYSRDELLAMRVEDLLVDGQDWTDEEYIRYLSEGPWLGE
                     IELRRKGGKKVPVEVLATVVELPTGPVYLSALRDVSERHRLERLRRDFLAMVTHDLRT
                     PLTTVRGNAQLLKRRAEYRPAQVDAVLAATAQMQQLINNLADVVV"
BASE COUNT          910 a         1406 c         1496 g          892 t
ORIGIN      
        1 gcaaagcagc agaacggaaa tgatcgcgaa tggaacacgc cgggcaatca gcgggagccc
       61 tccatgaaaa gcccacaatc gcgcaccagg gtgccgtcat cggtgaggat ggccggggac
      121 ggtgtccctg ggcggtgaca catccgctca atctcgccta ccacgacacc gagtggggaa
      181 tgccggtcca tggcgagcac gcgctcttcg aacggatcat gctggaggcg ttccagtcgg
      241 ggctgagctg gttgacgatc ctccgcaagc gcccggcttt tcgcgcggca ttcgccgggt
      301 tcgatcccga tatcatcgct cgattcactg aggacgacat tgtccggctg atgaacgatg
      361 ccggcatcgt tcgcaaccgt gccaaaatcc tggcggcgtg caccaacgcg caggcggtgg
      421 tggcgttgcg ggaacagggc ggactggacg agttgatctg gtcccacaag ccggaggtta
      481 cgccggcgcc ggagacgctt gctgacctcc tgacgcagac gtcggaatcg cgggcgctgg
      541 cgaagggcct caagtcgcat ggctttgtct ttgtcggccc aacgacggcc catgcgctca
      601 tggaagcaat cgggctcgtg gacacgcatt tgattggctg ccaccggcgc ggatgttccg
      661 gcgtgtttcc aacggtgaag gagggtggac cgtgaccacg gccgaggagc gagtcgagaa
      721 actcatcgtg aagctggatg cggcctggaa tgcgttccag gactcctatg ccgggctatc
      781 cgatgatgaa ctgcttgagc ccggtgtgac cggcgagtgg tcggtgcggg acctcattgc
      841 ccatattgcg tggtgggaag aggagtcgct gaagcacctg ccgctggtgc tggagggagg
      901 gcggccgcaa cggtattccg tgaagtacgg tgggattgac gcattcaacg ccctgatgac
      961 cggccagaag caagatctct tgcttgacga gattttccat gaatccgggg agacacatcg
     1021 tcgcctgatc gagtatgtcc ggagcatccc gcccgaggca ttcgtcggcg acacgcgtgc
     1081 ccgtcgccgc ctgcgcctcg atacctatgg ccattacccg atccacacga gggatatccg
     1141 ggagtggcgc gagcggcggg gcaaggctag gggcgagaac accgatccaa aatggaaaag
     1201 gcagacactc ttgtgaatat cgtcgagaca atatcgatga ccaccacgat gaaccaacag
     1261 acggcgcctg gtcgagagct attgccgttg tcgacgtata cgggcactac agggagatga
     1321 tgcccttgct gcaggattct ccttaccgtc aaagaagggg ccctttgagc gattgtcact
     1381 ccggaaacac caggtcatat tcctcgcata gtcgcccgat actctcatag tcgacttcca
     1441 ggccgtaacg ctcatcgact gccgcagcat gttctccggt gaaggcttcc ggatctgctc
     1501 ccatctcggc aaacatgcgc tcgaacccac cgggtgaaat gatctccagg atccggcacg
     1561 gcatatcccc ggcgttccag aacgtatgcc attgcccgcg aggtttgaaa acgaggtcac
     1621 cgggccctgc gtgaatcaca ttgtccccaa gcagtgcccc cacccgaccc ttcaggacat
     1681 agctgtactc atcttcctcc gtgtgaagat ggagtggtgc cgccagtgtg cgtggaggga
     1741 tggaatgctc gaccaacgag aagcctcctc cggattcctc tgcccacacg agaaaccgca
     1801 ctcccaacgc tcccagatcg accgaaggag cgttgaccga tgtcactacc cgtggggtgt
     1861 cggaggcgcg aacccgtcgt cgttcatcag gttgcggaac cggtgctgat gccatctgcc
     1921 ctccgtcctc tccagtggga acactgccat gtgcgtcaat cgagatctgg cctccaggcg
     1981 gcggttttgt ttaaactgac cgactctccg cctccgttgg ctctggaggt ctcccctgtc
     2041 ttgccaacgt accgctgccg cgctgcctga gcctcttcga gatggagtcc tcttggcagg
     2101 acatcgcgct gctatgggcg tcatggtagc ggactggtcc ctgtggagga agagtgtcag
     2161 gcggccaaat gagtgtgctg tgtaaccatc cgagttgtta ctctcacagc gtcccccctg
     2221 gcgcttgcag cagacccggt tgttccatca gggttggcct ccgctgagca gttgccgcgg
     2281 gagggcagcc ctgcatcccc gcctgctatt aatagtgatc aaataatagc gagacgcttc
     2341 attgttttca tttcaggtat tctgggacag atacgagaag gaacgtgggt caccgcgtgt
     2401 gagtacaacg gaaaggggga gccggaatgc acctcgccca gctcgaagca ggttggacat
     2461 tacttccctc ccgtgaatgc cacagagtac agctgtccgc ctccagcctc ctgatggcga
     2521 gcctatgaca cgctccaccc atccggtcac catcgagtca tgtcccacgc cgatgtggac
     2581 ggtgcggttg gacggcacca ttgagtacgt caatcctgcc tggggggcct actcgggtat
     2641 ctccggagag ggtgccgtcg atgagagctg ggtcgccgcc gtgcatccgg aagacgttgc
     2701 gtcgttcagg gacaggttcc agtcggctgc ccagaaaggc gaggcgtatc aggtcgagta
     2761 ccgcttccgt cgggcagccg gaacgttccg ctggcacttg gccaatatct cgccgctccc
     2821 cgacgcagat ggcgtcatca ggcggtggct gggaatcgcc atcgatattg atgatcggcg
     2881 gcgagaggaa gcggacttgc gcgccagtga agcccacttc cggggcactg tcgagtcggc
     2941 aggggacatc gtctataccc ttgctctcga cggtatcgtc acggcggtga atccggcaat
     3001 caggcgcgtt ctcggatata cgccggagga ggtacagggg cggtccatcg aagagacgct
     3061 tcttcctacg gatcagctgg acgttgcgcg agaggcgtta cagcgcaagc tggctggaga
     3121 ggaacggggt ccctacgagc tcgacgttct ggcgaaggat ggccgtcgcg tcaccctgga
     3181 gatcaatagc cggttgaccg tcgccggggg gcagcccggg gccatccagg gcatcgcccg
     3241 cgatgtcacc atgcgcacgc aaacgcagga gggacttgcc cggctggccg cgatcgtgga
     3301 ggactcggac gacgccatcc tgagcaggac cctggacggc acgatcacca gttggaacct
     3361 cggggcggag cggctctacg gatataccgc ggaagaggct gttggaggac atgtcacccg
     3421 gctggtcccg cctggccgag acgaagaagt tgatcagctt actgaccggt tggcacgggg
     3481 ggagcgtgtc ccgcattttg agacggtgcg cctgcgcaag gatggaaccc tgatcgatgt
     3541 ctcgatcagc ctttcacctc ttactgaccc tgacggccgg ctgatcggca tctccaccat
     3601 cgctcgtgac atcaccgcac gcaagcagtc agaagcgcgg ctgcgcctcc tggccgaggc
     3661 aggacgacac ctggcgtctg atccaaacgt cgatgcccaa ctcaccgctg tggcacgcct
     3721 ggcagtaccg acactggcgg actggtgtgt cgttgacctg cttgacaacg gatcggacct
     3781 gcgccgtgtt gcagtcgccc atgtcgatcc agtcaagctc gccatggcag aggagctaca
     3841 gctccgttac ccgcccgatc ccgcagctcc ccgaggacca catgaggtta tgcgaaccgg
     3901 tgagccggag ttcatgtccg acattccgga cgagttgctg gtggccgcag cgcatgacgc
     3961 cgagcacttg cgactcctgc gtgaggtggg cttgcgatcc tatctcaccg tgccgctgca
     4021 tgcgcggggc cgggttctcg gtacgctgac cttcattgca gctgaatccg ggcacgcgta
     4081 tggaccggac gacctgccaa tatgcgaggc gctggctgag cttgcggccc tggcgatcga
     4141 caatgctcag ctttaccgca atgcccagga agccgagatc cgctaccgga ccgttttcac
     4201 gggagtagcc gatgcgatcc ttgttgctga tacgaagcac cggtacctgg aggcgaaccc
     4261 ggccgcgact gaactcctcg gctacagccg cgacgagctg ctggcgatgc gagtggagga
     4321 cctgctggtg gacgggcagg actggacgga cgaagagtac atccggtatc tgtccgaagg
     4381 tccctggctg ggcgagatag agctacgccg caaaggtggg aagaaagtgc cggtcgaggt
     4441 ccttgcgacg gtagtggagc tgccgaccgg gcccgtgtat ctgtcggcac tgcgtgatgt
     4501 ctccgagcgt catcggctag agcggctgcg acgagatttt cttgcgatgg tgacccacga
     4561 tctccgcact cccttgacga ccgtaagggg taatgcacag ttgttgaagc gacgggccga
     4621 gtaccggccg gcgcaggtgg atgccgtgct cgcggccact gcccagatgc agcaactgat
     4681 caacaacctg gccgatgtcg tggt
//
LOCUS       DASYWM010000089         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_585037, whole genome shotgun sequence.
ACCESSION   DASYWM010000089 DASYWM010000000
VERSION     DASYWM010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585037"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VGR29_03635"
     CDS             <1..246
                     /locus_tag="VGR29_03635"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV2072712.1"
                     /translation="IFPWTAAFALGFLSHLLSDAWTHAGVRPLLPFSDTRVWIAPKLL
                     RGKSDGPLDALVRLAAMLALLARVATYAAAHLVIPTF"
     gene            complement(286..933)
                     /locus_tag="VGR29_03640"
     CDS             complement(286..933)
                     /locus_tag="VGR29_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2072713.1"
                     /translation="MRSTHRPVASSTVAIAAAVIAAILGVVLGMVAAGDNVLPGDVPV
                     ADAVQQLDGRMFEILARIGNTIGSTLWAAAAILLMIAVAAMWRAWPEVIYLVTLLVLR
                     LLGTLLKPVFNSPRPTDDLVLITGTHDGTGYPSGHAFTAATMALGLAVLAWRHIPSRA
                     MAMVAFLTGLTVLIGWARIWTGAHWPSDVLGGFAFGVAVVAVGVVVLNRVSHTQP"
     gene            1224..3143
                     /locus_tag="VGR29_03645"
     CDS             1224..3143
                     /locus_tag="VGR29_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072714.1"
                     /translation="MVQSSSSLGRLYESLKDGSISRRDFLQRASATGIGAGAALFLAN
                     SAVIAANGGSRNGLAVYQGADGTPAASPTDGEYKLLDSGTENQTRGEGGVLRLIQWQA
                     PTMAAPHSAVGTKDYLAASLVIEPLLTYMPDGSIIPVLASVVPSVENGLLSENLTEVT
                     FPLKEGVVWSDGEPFTAQDVVFTWQWIVNPANASVSAEVWQNVAEIVAVDDLTVKATY
                     MQPVAAWFEPFTGDIYGGIYPAHVFNNDVNNKNESFLTNPIGTGPFVIESFAPNDQVT
                     YVANENYREPNKPFFSSAVIKGGGDAAAAARSVCQTGEYHYAWNLQVEPNVLQQIDEE
                     GEQGNIRVEVGTAVERIHINFSDPWTEVDGQRSHFGTPHPFLTDDAVRQAMNVACDRE
                     TIATEFYGEGQPPTANILTGLELFESPNTSWEFNLEKAAQILEDAGWVMDGDVRAKDG
                     VELSVVYATSVNAVRQKTQAVIKQAFEQIGIKVQLEQIDAGIFFDSGAGNEQNISHFY
                     WDIDMYTNNPGSPVPVSFMLSWYAGETGDNVAQESNSWQGQNYQRWANEEFDQLFEEL
                     QVATDLDTAAQMLIDLNDILINNVVIIPLVNRAADVFAYSTLLNLENLQIGPFNENYW
                     NIANWNFAEGVEPQS"
     gene            3580..>4133
                     /locus_tag="VGR29_03650"
     CDS             3580..>4133
                     /locus_tag="VGR29_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072715.1"
                     /translation="MVSQNHASGPLRALYEGLKAGNIDRRTFVHRASLLGISAASAVF
                     LANTAGVAAQDASPAATAAAERPAAGTENQERGAGGELKLIQWQAPTLLSPHVATGVK
                     DFLASVLVLEPLMHYLPDATMIPNLVTEVPSVENGSLSEDLTEVTLKLLPDVVWSDGE
                     PFTAEDVVFTIDWVKNPENASVNI"
BASE COUNT          833 a         1278 c         1223 g          799 t
ORIGIN      
        1 atcttcccct ggacggccgc cttcgccctc ggcttccttt cccatctcct cagcgacgcg
       61 tggacccacg ccggtgtgcg gccgctgctg cccttctccg atacacgtgt ctggatagcg
      121 ccgaagctgc tgcgcggcaa gtcagacggc ccgctggacg ccctcgtccg cctggcggcg
      181 atgctcgccc tgctcgccag ggtcgcgacg tacgccgcag cacacctggt gataccgacg
      241 ttctgaagga caagtcgacg ttgcagggga gggcaaggtc cttccctacg gttgggtgtg
      301 cgacacccgg ttcaacacca ctacgccaac cgccacgacc gccacgccga acgcgaaccc
      361 cccaagcaca tcggacggcc aatgcgcccc ggtccatatc cgcgcccatc cgatgagcac
      421 cgtcagcccc gtgaggaacg ccaccatcgc catcgcccgc gaggggatgt gccgccatgc
      481 gagcaccgcg agaccgagtg ccatggtggc cgcggtgaag gcatgtccgc tgggatagcc
      541 ggtgccgtcg tgcgtccccg tgatcaggac gaggtcgtcg gtcggcctgg ggctgttgaa
      601 gaccggcttc aggagcgtgc cgagcaggcg caggaccagc agcgtgacga gatagatgac
      661 ctcgggccat gcgcgccaca tcgccgcgac ggcaatcatc agcaggatcg cggcggctgc
      721 ccacagggtc gagccgatcg tattgccgat acgggccaat atttcgaaca tgcggccgtc
      781 gagctgctgc actgcatcgg caaccgggac atcgcccggt aggacattgt cgcccgccgc
      841 gaccatcccg agaacgaccc caaggattgc tgcgatgaca gccgcggcga tcgccacggt
      901 ggaggatgcg accggtcggt gtgttgatct catgaattct cctggcatgg atctatttct
      961 caaacaacat atgttcataa tctcattacg atcgccggtt gctgtttctt caggaaatct
     1021 tcatatttac gaccgttaag tagctccttg catcgttcgc catcatcccc tactatttct
     1081 cccgtgtctt gagtcacaag tcaatgtttg gttgcagtag atttgttgtg acgccatacg
     1141 ctcttacatt ccgggtggaa ggttgggcgg cgttcacgta accaacgtct ggaagatacc
     1201 aggcaacgta ggagaatcga cgaatggtcc agtcctcaag ctcgcttggt cgtctctatg
     1261 agtccctcaa ggacggcagc atctcacgac gtgacttcct gcagcgcgcg agcgcgacag
     1321 gcattggtgc tggcgcagca ctcttcctcg ccaactcagc cgtcatcgcg gccaatggcg
     1381 gatccaggaa tgggctcgcg gtctatcaag gcgcggacgg gacgcctgca gccagcccaa
     1441 ccgacggcga gtacaagttg ctcgattccg gtacggagaa ccagactcgt ggcgaaggcg
     1501 gcgtgttgcg gctgatccag tggcaggccc cgacgatggc tgcaccccac agtgccgtcg
     1561 gtaccaagga ctacctggct gcctccctcg tgatagagcc actgcttacc tacatgcccg
     1621 atggctcgat catcccggtg ctggcatccg tcgttccatc cgtggagaac ggtctgctct
     1681 ccgagaacct gacagaggtt accttccctc tgaaggaggg cgtggtctgg agcgacggcg
     1741 agccctttac ggcccaggac gtcgtcttca cctggcagtg gattgtcaat cccgccaatg
     1801 cctcggtcag cgccgaggtc tggcagaatg tggcggagat cgtggcagtg gatgatctca
     1861 ctgtcaaggc aacctacatg cagcccgtcg cggcctggtt tgagcccttt acaggagaca
     1921 tctacggggg aatctatccg gcacatgtct tcaacaacga cgtcaataac aagaacgagt
     1981 cgttcctgac caacccgatc ggcaccggcc cgtttgtcat cgagtccttc gctccgaacg
     2041 accaggtcac ctacgtcgca aacgagaact accgggagcc aaacaagccc ttcttctcga
     2101 gcgcggtgat caagggtggt ggcgatgcag cggcggcagc ccgctcggtc tgccagaccg
     2161 gtgagtacca ctacgcctgg aacctccagg tggagccgaa cgtcctgcag cagatcgatg
     2221 aggagggcga gcagggcaac atcagggtgg aggtaggcac tgccgttgag cgcatccaca
     2281 tcaacttctc cgatccctgg accgaggtcg atggtcagcg gtcgcacttc ggtacgccgc
     2341 acccgttcct cacggacgat gcggttcgcc aggcgatgaa cgtcgcgtgc gatcgcgaga
     2401 ccatcgcgac cgagttctac ggcgaaggac agcccccaac ggccaatatc ctgaccggtc
     2461 tcgagttgtt cgagtcgccg aacacatcct gggagttcaa cctggagaag gcggcacaga
     2521 tcctcgagga tgccggttgg gtgatggacg gcgatgtccg cgccaaggac ggcgtggagc
     2581 tgagcgtggt ctatgcgacc tccgtgaacg cggtacgcca gaagacgcag gccgtcatca
     2641 agcaggcctt tgagcagatc ggcatcaagg tccagcttga gcagatcgat gcgggtatct
     2701 tcttcgatag cggcgcgggc aacgagcaga acatctccca cttctactgg gatatcgaca
     2761 tgtacaccaa caacccgggg tcgccggttc cggtgtcgtt catgctgtcg tggtacgccg
     2821 gtgagactgg agacaatgtc gcccaggagt ccaactcctg gcagggacag aactaccagc
     2881 gctgggcgaa tgaagagttc gatcagctgt tcgaggagct tcaggtcgcg accgatctgg
     2941 acacggcggc ccagatgctg atcgacctga acgacatcct catcaacaat gtggtcatca
     3001 ttccgctcgt gaatcgcgcc gcagatgtct tcgcctactc cacgctgctg aacttggaga
     3061 acctgcagat cggcccgttc aacgagaact attggaatat cgcgaactgg aacttcgccg
     3121 aaggcgttga gccccagtcc tagcactttg ttacatcatc cgggatggct cgtcccgggt
     3181 gatgtaggca cccacgatcg aaagatgaag aacgagcggc gtcacgatat gtggcgccgc
     3241 tcgttcttct gtcatgtcgc ctagcgaccg tgccagcagc cagagggtcc gctggctccg
     3301 gtgccctcgt gtacttacgc caatatgtgc ttttgataca gagcaagctc tggtccttgg
     3361 tgcggcatca ttcgtgccct gtttcacgag ccattgtcaa tggcccccgg gatgattaaa
     3421 cttctcgcgt gagtcaacat ctgggtaact ggtagccttc cagatactcc cggtcgtcgc
     3481 atagccaaca gacccggata atgactcgga ctccatcagg gaacactttc cccgatgatc
     3541 acaataccgg tacgacgccg gcaggaggaa cgaccaagaa tggtctctca aaatcatgca
     3601 tccggtccgc tgcgagcgct ctacgagggc ctcaaggcgg gaaacattga tcgacgtacc
     3661 tttgtccatc gcgcatcact gcttggtatc agcgccgctt cggcggtttt ccttgcgaac
     3721 acggccggcg tcgccgcgca ggatgcatcg ccggcggcaa ccgccgcagc cgagcgcccg
     3781 gctgctggca ccgagaacca ggagcgcggc gccggtggcg agctgaagct catccagtgg
     3841 caggcgccga cattgctcag cccgcatgtg gcgacaggcg taaaggattt cctcgcatcg
     3901 gtgctcgtac ttgagccgct catgcactat ttgcctgatg cgacgatgat cccgaatctt
     3961 gtcaccgaag tgccgtccgt ggaaaacggc tccctgagtg aggacctgac ggaagtcacc
     4021 ctcaagctgt tgccagatgt tgtctggagc gatggtgaac ctttcaccgc agaggacgtg
     4081 gtgttcacta tcgattgggt gaagaacccg gaaaacgcct ccgtgaacat caa
//
LOCUS       DASYWM010000090         4045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_321951, whole genome shotgun sequence.
ACCESSION   DASYWM010000090 DASYWM010000000
VERSION     DASYWM010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321951"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VGR29_03655"
     CDS             complement(<1..1090)
                     /locus_tag="VGR29_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2072716.1"
                     /translation="MTEQNTRMKWVDLVSRDDIVDVGTKLVSIPSVSGDEYDIMQFVK
                     GWLDGLGIDFVETAKDPRRPNVIATVGDASKGPIVAMNGHLDVVPVSDASSWKTDPFE
                     GAVSEDGTRIYGRGASDMKTSVGVMMTMLKLFKDAPLQGALQAHIVSDEETSAEFGTK
                     HVLAEIEKGNLPRPDYVFIGEGSEHKVRNAERGGLAVNVKFIGKASHTAAARAGGINA
                     LQKAAKGILALEHHLEQFHPAVGHPVLSVNMIEAGIAHNVVPGEATISIDRRLIPSEN
                     KESAVADICAKLDAVAAEDPDFKYELEIDPDGSYIDPNITPEDSPLVQAFQESIRTLT
                     GEEPEFFVQWAGMTDGRFYRQHGMDTVGM"
     gene            complement(1213..1542)
                     /locus_tag="VGR29_03660"
     CDS             complement(1213..1542)
                     /locus_tag="VGR29_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEV2072717.1"
                     /translation="MKLVITIVQDEDTDILTEALISAGFRFTKISTTGSFLRTGNTSL
                     MIGVEDAGVPAVMNILQRTCRRRTQIAVPYSPALEPGLLYMPENFEVEVGGAIVFVAN
                     VSRFERI"
     gene            complement(1771..2598)
                     /locus_tag="VGR29_03665"
     CDS             complement(1771..2598)
                     /locus_tag="VGR29_03665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV2072718.1"
                     /translation="MDDLNTGTVDQDVLRKARNLAERTAYEAGALLRERVKTERVVTH
                     KGTVDLVTDADEESERLISSALAEAFPDFRLTGEEGATGASESDYGWLIDPIDGTTNF
                     AHGYPHFAVSICLEFQGVPVVGVVYDPMRDEMFTGAEGQGATLNGVPIRVSKTETLIG
                     ALGATGFSYDVGAREEGAALWATFNDRLQGLRRDGAAALNMVWVAAGRLDAYWERPTN
                     AWDIGAGVVIARESGATVTSLEGHPYQLHETEVTCTNGLLHEQVVTLVRETLAELRG"
     gene            complement(2607..3704)
                     /gene="tsaD"
                     /locus_tag="VGR29_03670"
     CDS             complement(2607..3704)
                     /gene="tsaD"
                     /locus_tag="VGR29_03670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV2072719.1"
                     /translation="MNILGIETSCDETAAAVVTDGRVVHSNVISSQIELHRAHGGVVP
                     ELAARGQVTAILPVVRAALVEAGMTTDDIDAVAFTQGPGLAGSLLVGVNVAKAYAYAL
                     GKPMIPVNHLEAHVYANWLQTPEDTAFIAPEFPAVCLLVSGGHTEILLLHGHNRYEVL
                     GRTLDDAAGEAFDKGARLLGLGYPGGPAIQQASQGGSPDAFDLPRAWLPGTHDFSFSG
                     LKTALLRTAQGYRLPDLESGPTGQLFPEHRPPRYRDDLPLADLAASFEEAIVDVLATK
                     LVKAAIDCGARSVLIAGGVAANRRLRERIAEQVGGNAQVRWPPMELCTDNAAMIAGRA
                     WQVAQMDAWGNLETDAYPRLPIGNENAVAKG"
     gene            complement(3728..>4045)
                     /locus_tag="VGR29_03675"
     CDS             complement(3728..>4045)
                     /locus_tag="VGR29_03675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2072720.1"
                     /translation="GVRPDYQGRGLGELLFLQLIDESIRRKATWLTLEVRVTNYGAQA
                     LYRKYEFSIQGRRPRYYSDNNEDAFVMWSESFRDPEYLKRIETHRRRLHHKLSFVNEP
                     AES"
BASE COUNT          753 a         1333 c         1150 g          809 t
ORIGIN      
        1 ccatcccaac cgtatccatg ccgtgctgcc ggtagaaccg gccatcggtc atgcccgccc
       61 attgcacgaa gaactcgggc tcctcccccg tcagcgtccg gatgctctcc tggaacgcct
      121 gcaccagcgg cgaatcctcc ggagtgatgt tggggtcgat gtaggagccg tccgggtcga
      181 tctccagctc gtacttgaag tccggatctt ctgccgcaac tgcatccaac ttggcacaga
      241 tgtccgcgac agcgctctcc ttgttctcgc tagggatcaa tcgccggtcg atagatatcg
      301 ttgcctcacc ggggacgacg ttgtgcgcga tcccggcctc gatcatattg accgagagta
      361 ccggatgccc cacagcgggg tggaactgct ccaggtgatg ctccagtgcg aggatgcctt
      421 ttgcagcctt ctggagcgca ttgatgccgc ccgcgcgggc agctgccgtg tgcgatgcct
      481 tgccgatgaa cttcacgttc accgcgagtc cgccccgctc tgcgttccgc accttgtgct
      541 cggacccctc accgataaat acatagtcag gacgcgggag gttgcccttt tcgatctcgg
      601 ccagcacatg cttcgtgccg aactccgcac tggtctcctc gtcagagacg atgtgtgcct
      661 gcagcgcacc ctgcagcggc gcgtccttga agagcttgag catcgtcatc atcacgccga
      721 ccgaggtttt catgtctgac gcaccacgtc cgtagatacg cgtgccatcc tcactgaccg
      781 cgccctcgaa gggatcggtc ttccacgatg aggcgtctga taccggtacg acatcaagat
      841 ggccgttcat ggcaacgatc gggcccttgc ttgcgtcccc aacggtagca atgacgttgg
      901 gtcgccttgg atccttcgcc gtctcgacaa agtcgatacc tagcccgtca agccagccct
      961 tcacgaactg catgatgtcg tattcatccc cgctgacact ggggatcgaa acgagcttgg
     1021 ttcccacatc gacgatatcg tcgcgcgaga cgagatcaac ccatttcatc cgggtatttt
     1081 gttcggtcat tccctgttcc tcctgggacg cacgtgcgcc ttcgaatctt ccgacccact
     1141 tgtaccacgc gaaagggccg ctcccaacag gaagcggccg accaggcgcg atgacttgag
     1201 agaagaatgg gatcagatgc gctcgaaacg ggagacgttg gcgacgaaga cgatcgcacc
     1261 acccacctca acctcgaagt tctcaggcat atagagcagc cctggctcca gcgcgggcga
     1321 gtacggcacc gctatctgcg ttcggcggcg acaggtgcgc tgcagaatat tcatgaccgc
     1381 gggaacgccc gcgtcctcaa caccgatcat gaggctggta tttccggtac gaagaaacga
     1441 cccggtggta ctgatcttcg taaaccggaa cccggccgat atcagggcct cggtcaggat
     1501 atcggtatcc tcatcctgga caatcgtgat gacgagtttc acaggagtgt tcctccctgt
     1561 atcagcttca tgcgcggcgg atcgtgttct ggcgaccctc ccggctctct gggtgaccac
     1621 ttatcagcac cggagatggc cggtggaaaa tcagaaccag tgcttgcaca agcggccgca
     1681 ccccgcgact tcccgggccc tgtcttccgg tttcccgtca ccggtgttgt gtggagctac
     1741 tttacacgaa cggaggttgc cccgatgctc ctatccgcgc agttcggcca atgtctccct
     1801 tacgagggtc acgacctgct cgtggagcag tccgttggtg caggttactt cagtttcatg
     1861 gagctggtag ggatgtccct ccagcgaggt cacggtcgcc ccggactcac gagcaatcac
     1921 tactccggcc ccgatgtccc aggcattagt cgggcgctcc caatacgcat ccagccgtcc
     1981 tgccgccacc cacaccatgt tgagggccgc cgcgccatcc cggcgcaggc cctggaggcg
     2041 atcattgaac gttgcccaca gtgctgcgcc ttcctcccgg gcgccgacat cgtagctgaa
     2101 gccggtcgcg cccaaagcgc cgatgagtgt ctccgttttg gacacacgga tcggcacccc
     2161 gttcagggtc gccccctgcc cctcggctcc agtaaacatc tcgtcgcgca tcgggtcgta
     2221 gacgacaccc acaaccggga ctccctgaaa ctcaagacag atggaaaccg cgaaatgggg
     2281 atagccgtga gcgaagtttg tggtgccatc aatgggatcg atcaaccagc cgtagtcgct
     2341 ttcggatgcg ccggtcgctc cctcttctcc agtgagccgg aaatccggga atgcctctgc
     2401 cagcgcactg gaaatgaggc gctccgactc ctcatcggcg tccgtgacaa gatcgacggt
     2461 acccttgtgc gtgaccaccc gctcggtttt gacccgctcc cgcaggagcg ctcccgcctc
     2521 gtacgcggtc cgctcggcga gattacgtgc ttttcgcagt acgtcctgat ccacagtgcc
     2581 tgtgttcagg tcatccatat cccggctcat cccttcgcta ctgcattttc gttcccaatc
     2641 ggcagacggg gataggcatc cgtttccaga ttgccccacg catccatctg cgccacctgc
     2701 cacgctcgtc cagcaatcat tgccgcgttg tccgtgcaaa gctccatcgg tggccagcgc
     2761 acctgcgcat tgccaccaac ctgttcagcg atgcgctctc gcagccgccg atttgccgca
     2821 acgccgcccg cgatcaagac ggaccgagcg ccacaatcga tcgccgcctt gaccagcttg
     2881 gtcgcgagca catcgacgat cgcctcctcg aaggaggcgg caagatccgc gagcggcaga
     2941 tcgtctcgat atcgtggggg ccgatgctcc gggaagagtt gccccgtcgg tcccgactcg
     3001 agatcaggca ggcgatagcc ttgggctgtg cgcaaaagcg cagtcttgag gcccgagaag
     3061 ctgaaatcgt gcgttccggg aagccaagcc ctgggcaaat cgaaggcatc cgggctcccg
     3121 ccctgggacg cctgctggat ggccggccct cccgggtatc caagaccgag caagcgtgcg
     3181 cccttatcga aggcctcgcc tgcggcatca tccagcgtcc ttccaagcac ctcgtaccga
     3241 ttgtgcccgt gcagcagcag gatctcagta tggcccccgg acaccagcag gcagactgca
     3301 ggaaactccg gagcgatgaa agcggtgtcc tctggcgtct ggagccagtt tgcgtacacg
     3361 tgcgcctcaa gatgattcac cgggatcatt ggtttgccaa gggcgtatgc gtacgccttg
     3421 gcgacattca ctcccacgag gagggaaccg gccagccctg gcccttgggt gaaggccacg
     3481 gcatcgatgt cgtcggtcgt cattcctgcc tcaaccagcg ccgcccggac cactggcagg
     3541 attgccgtga cctgcccgcg cgcggccagt tccggcacga caccaccatg cgctcggtgc
     3601 agctcgatct gcgacgagat cacgttggaa tgcaccacac ggccatcggt gacgacggcc
     3661 gcggcagtct cgtcgcacga cgtttcgatc ccgaggatgt tcatgatgga ctactttctt
     3721 ggcggagtca ggattctgcc ggctcgttca cgaagctcaa tttgtgatgc agcctgcgac
     3781 gatgcgtttc gatgcgcttg aggtactccg ggtcccggaa ggactccgac cacataacga
     3841 acgcgtcttc gttgttatcg ctgtagtatc gcgggcgacg cccctggatc gagaattcat
     3901 atttccggta gagcgcctgg gcaccgtagt tggtgactcg aacctccagc gtcaaccagg
     3961 tcgccttgcg acgaatggac tcgtcgatca actgcaggaa gagcagctcc cccagtccgc
     4021 gaccctggta gtcgggccgt acgcc
//
LOCUS       DASYWM010000091         4177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_416111, whole genome shotgun sequence.
ACCESSION   DASYWM010000091 DASYWM010000000
VERSION     DASYWM010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416111"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VGR29_03680"
     CDS             <1..904
                     /locus_tag="VGR29_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEV2072721.1"
                     /translation="AGDPFGFGGAGSPFGDLFETFFGGAAGGANGRRSAPVRGADIQV
                     SVNLTFEEAVFGVEKEVEVDRLETCDVCEGTRMKDGKTPPSCPSCGGSGEVRRVQQTI
                     LGQFMTSGPCPTCRGEGVTISDPCENCKGRGRVRNYRTLSVTIPPGIDDNATLRLSGQ
                     GEDAPNNGAAGDLYVKVRVQQHKFFERHGKAVHSRVGINVAQAALGDEIEIDTLDGPV
                     VFRVPPGTQSGQQFRLRSKGVPDLRGGERGDQIVTVQVIVPKKLTEEQEELFERLSAS
                     LGREVTQQPKGRGFFDRVKDAIGV"
     regulatory      1001..1148
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1433..2377
                     /locus_tag="VGR29_03685"
     CDS             1433..2377
                     /locus_tag="VGR29_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072722.1"
                     /translation="MLISIAKRIIQMVLTLTLASTVIFVLIHLSGDPTQGFMPAGASP
                     EVRDATRARLGLNDPILEQYLRFVTNGLTGNFGDSWRDRQPAMDAVLDRLPATLLLAG
                     TALAAAVIGGTVVGVISASMGSHVLLHAVRVIPVAGQAVPTFWLGAMLILLFAVKLGW
                     FPSSGSGSPSSLVLPAVTLASQPGSVIARLISTGMRQLQRSEFVRTAESKGLRPSTIA
                     VRHVLPNAILPVLAYVGLQAGFLVGGTVVIESLFAWPGIGRLALQSAIERDLPVIHAF
                     VVVTAVGVIVINLGVDLIRLMVDPRQRTGMVDGRLANG"
     gene            2370..3281
                     /locus_tag="VGR29_03690"
     CDS             2370..3281
                     /locus_tag="VGR29_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072723.1"
                     /translation="MAEPPASLAEPSAFAPTERERPHQRVNPWLGVRRQAVAQVLPLL
                     AVVVLASLGPSLLPLSATDQDLLGRLQPPVWLDGGDWNHPLGTDGLGRDLLARIASGA
                     RTSLIVSIVAAAGAGVIGVTAGVLGGMRGGLTDRIITMLVETLLAVPFITVGIVVTGT
                     VGQSQTNLLLLLVLSGWVTHARIVRLQVQSLVRSDFILAAIAMGAVRTHVALRHLLPN
                     LAPVIIVVLFQQAGLMLLWSASLTYLGIGLPIDEISLGGIVREGQELIYNAWWVSVFG
                     GLAIALAVTGFNLFADWLQRRVDPTLR"
     gene            3353..>4177
                     /locus_tag="VGR29_03695"
     CDS             3353..>4177
                     /locus_tag="VGR29_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072724.1"
                     /translation="MTMGTAMTRRSLIGIGGSAGIRLLAPRAQVMPRQETPEAELLQE
                     LVIDLAGDPVSIDPAFAYSARDWSIVHSMYDGLIAIGDDGTIQPLAAETFDVIDDLTF
                     EAKLREGLTFHDGSPVTADAAIRGIEHLQRSEDSQISALFSSITELKRVDDLTVQILC
                     DAPSPWLPAQMAVWHMLLPEGYTAESLLTSPIGSGPYRFVSWEKGSHVELERFEEYQP
                     TNVKGAPIAERVIYRFVPEVSTRVADLLSGSADIAVDIPLDQHEAVESGEATIISEP"
BASE COUNT          760 a         1192 c         1324 g          901 t
ORIGIN      
        1 agccggcgat cccttcggat tcgggggtgc cggttcgccc ttcggcgatc tctttgagac
       61 gttcttcggc ggagccgctg gcggcgccaa tggcaggcgc tcagctccgg ttcgcggggc
      121 cgacattcag gtatcggtca acctgacctt cgaggaagcc gttttcggcg tggagaagga
      181 ggtcgaggtt gaccgcttag agacctgtga tgtctgcgag gggacgcgga tgaaggatgg
      241 gaagacaccg ccttcatgcc ccagctgcgg cgggtccgga gaagtacggc gcgtccagca
      301 aacgatcctg gggcagttca tgacttcagg tccctgtccg acatgtcgtg gcgaaggtgt
      361 cacaatctcc gacccatgcg aaaactgcaa gggcagggga cgggtacgca actaccgtac
      421 gctgtcggtg accatcccgc cggggatcga cgacaatgca actctccgcc tcagcggcca
      481 aggcgaagat gctccgaaca acggtgcggc aggcgatctc tatgtcaagg tccgtgtcca
      541 gcagcacaag ttcttcgagc ggcatggcaa ggcggttcat agtcgcgttg gtattaacgt
      601 ggcacaggcg gcgcttggcg acgagatcga aatcgatacc ctggacgggc cagttgtctt
      661 tcgggttcca cctgggaccc agtctggtca gcagttccgg ctgcgcagca agggcgtccc
      721 tgatcttcgc ggtggtgagc gtggtgacca gatcgtgacg gtccaggtca tcgtcccaaa
      781 gaagctgaca gaggagcagg aagagctctt cgagcgtttg tcagccagcc tcggccgtga
      841 ggtgacacag cagcccaagg gccgcgggtt cttcgatcgg gtcaaggacg ccataggcgt
      901 ttagctttcc acgggagtga tcgtgaggag cgaccgctgg gtacacccaa cggtcgctca
      961 cagttgacgc ttcctcgctc agaccgtaaa gttgattgaa catagccttc ggggaatggt
     1021 gaggtcgtcg agctatggcg atcaagtcct gaccggcggt acagcccgca agcgcaaatg
     1081 gtcgcatgag cgcacgatcc ggtgaaactc cggggccgac ggtaaagtcc ggatgggaga
     1141 aggtgtcaat acgagcgacg gtctgccgga acctccggta gatccgctta gggtccggtg
     1201 tgcggcatat ccgtccattc gcggctcata agccgctcgg ttgccgctcc acaatgtctc
     1261 tacccgaggg cctccggctc ccgggtcttt tgtgcccgga cgttttggac accggaggca
     1321 cctgggagcg ttgtgtgctg cagatggcag ggtaagttcc gcggaacgcg aactttctat
     1381 cccgcatttc tcgcactcgt acgctcctcg cgtgtcctcc ttcaaggcac acatgcttat
     1441 ctcgatcgct aaacgaatta tccagatggt cctcacgctg acacttgcgt ccacggtgat
     1501 cttcgtgttg attcatcttt caggggatcc gacccaggga ttcatgcccg ccggtgcatc
     1561 accagaggtg cgggacgcaa ccagggcgcg gttggggctc aacgatccaa tccttgagca
     1621 gtacctgcgc tttgtcacca acggcctgac tggcaacttt ggtgattcct ggcgtgaccg
     1681 gcagcccgca atggacgcgg tgcttgaccg tttgcccgcc acgctcctac tcgcgggaac
     1741 ggccctggca gccgcggtga ttggcggaac cgtggttggt gtcatttccg cttcgatggg
     1801 gtcacacgtc ctgctgcatg cggtccgggt tattccggtt gccgggcaag cagttccgac
     1861 attctggctt ggagcgatgc tcatactgct gttcgcggtg aagttgggat ggtttccgtc
     1921 ctccggcagc ggctccccgt cgtcattggt gttacctgca gtgaccctgg catcccaacc
     1981 aggatcggtc atcgcccggc tcatctcgac cggcatgcgg cagcttcaac gaagtgaatt
     2041 cgtgcgcacg gccgagagca aggggcttcg gccctccact atcgcggtgc gacatgtgct
     2101 tccgaatgca attctcccgg tactcgccta tgtgggtctc caggccggct ttctcgtcgg
     2161 tggaaccgtc gtcattgagt cgctctttgc ctggccgggg atcggacgtc tggccttgca
     2221 atcggcgatt gagcgtgacc ttcctgtcat ccacgcgttt gtggtcgtga cagcggtcgg
     2281 cgtcatcgtc atcaaccttg gtgtcgatct gattcgcttg atggtcgatc cccggcaacg
     2341 cacgggcatg gtggatggga gacttgccaa tggctgagcc gcccgcgagc ctggcagaac
     2401 catcagcttt cgctcccacc gaacgtgagc gaccccacca gagggtcaat ccctggttgg
     2461 gcgtacggcg tcaggcggtc gcccaggtgt tacctctgct agccgttgtc gtactcgctt
     2521 ctttgggtcc gtcgctcctg ccgctctcgg ccaccgatca ggatctgctg gggcgattgc
     2581 aaccgccagt gtggcttgat ggcggtgact ggaaccaccc tctgggcacc gatggtctgg
     2641 gacgggacct gctggcccgt atcgcttcag gtgcacgcac ctcgctgatt gtcagcatcg
     2701 ttgccgcggc aggcgccggg gtgatcggtg tcaccgcagg agtcctgggg gggatgcgag
     2761 gtgggctcac cgaccggatc atcaccatgc tggtagagac gttgcttgcg gtgcctttca
     2821 tcacggtggg aatcgtggtg acgggaaccg ttgggcaaag ccagacgaac ctgttgctcc
     2881 tgcttgtctt gtcgggatgg gtcacccacg cccggatcgt gcgtctgcag gtgcagtcac
     2941 tggtcagatc agatttcatc ctcgcggcga tcgccatggg ggcggtccgc acccatgtcg
     3001 cactgagaca tctgttgcct aacctggcac cggtcatcat tgtggttctt tttcagcagg
     3061 ctggtttgat gctgctctgg agtgcctccc tcacgtatct gggcatcggt ctccctattg
     3121 acgagatcag ccttgggggc atcgtccggg aaggacagga actgatctac aacgcgtggt
     3181 gggtaagcgt ttttggagga ctagcaatcg cgcttgcggt cacaggattc aacctattcg
     3241 cggactggct tcagcgacgg gtggatccaa cgttacggta gatggaagga ctgacagcgt
     3301 gatgcagaag acccacgaaa cacagatcga aagttatata cggaggaatt tcatgaccat
     3361 gggaaccgca atgacccgcc ggtcattgat tggaattgga gggtcagccg ggatcaggtt
     3421 gcttgcgccg cgtgcacaag tgatgcctcg tcaggaaact ccggaagcgg aactcctgca
     3481 ggaactggtg atcgacctcg ccggagatcc tgtcagcatc gacccggcat tcgcctattc
     3541 cgcgcgggat tggtcaatcg tgcattcgat gtacgacggc ctcattgcca tcggcgatga
     3601 tggaacgatc cagccactgg cggccgagac ctttgacgtc attgatgatc tgacgttcga
     3661 ggcgaagctg cgggaagggc tgacctttca cgacggatca cctgtcaccg cagacgcggc
     3721 catccggggc attgagcatc tgcagcgcag cgaggactcg cagattagcg cgctcttcag
     3781 ctccatcact gagctgaagc gggtagatga tctgaccgtg caaatcctct gcgatgcgcc
     3841 cagtccgtgg ctcccggcgc agatggcggt gtggcatatg ctccttccag aggggtacac
     3901 ggcggagtcc cttttaacca gcccgattgg atcgggacca taccggtttg tctcctggga
     3961 gaagggaagc catgtcgagt tggagcggtt tgaggaatat cagccgacca atgtgaaagg
     4021 ggctcccatt gccgaacggg ttatctatcg ctttgtgccg gaggtttcga cacgtgtggc
     4081 tgatctgtta agcggctcag ctgacattgc tgtggatatc ccgctcgatc agcatgaagc
     4141 tgtggaatcg ggtgaggcga caatcatttc ggagccg
//
LOCUS       DASYWM010000092         4947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_509709, whole genome shotgun sequence.
ACCESSION   DASYWM010000092 DASYWM010000000
VERSION     DASYWM010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509709"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VGR29_03700"
     CDS             complement(<1..318)
                     /locus_tag="VGR29_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2072725.1"
                     /translation="MLSTGSPIQNRIGNVFVPVSDMPRAVAWYSRLLGLEEGQTSHQG
                     GIYDVPMAGETGLILDANKPITGHSAQPLCLFLTNDIAASIDWLRELGVEIAGEPQDI
                     GSVI"
     gene            complement(361..1452)
                     /locus_tag="VGR29_03705"
     CDS             complement(361..1452)
                     /locus_tag="VGR29_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEV2072726.1"
                     /translation="MNDTSPALVEALDSAVRTATERVRPNILLLSGGLDSSLLAAMWK
                     RAGHRFRAVTVGLDPSTRCSREHRSLPYPCNSDIAWAEKVADALDLDWTPVILSEQEA
                     LQSLDWLILQQRSFDLGQLNNIPLIAGLESAKGFDGTSFATGDDGDGLFGGYRFLRHQ
                     GDWPTYVRERIPYIDPPARSIGMASGWDPCFPYLEPEVLDIAQSCTWDDLSQMRSPTD
                     HPLPPSFMDQFDPQIMKATNRPWGKVLLRKVAERWLPREIAWRPKTDLQFGSGMCALE
                     SLLALRVGPDVRLRLDANGVHWLNDAHRGLYLRFLALGATVPEPTSGEYGCRSCGGGV
                     PIGKRHCATCGCWPADESPETAEPSADRL"
     gene            1488..2396
                     /locus_tag="VGR29_03710"
     CDS             1488..2396
                     /locus_tag="VGR29_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2072727.1"
                     /translation="MDIGLILPLAEDESGTAPSWAELRAAAVQAEGLGFDSVWVYDHL
                     LHRFEGHPTVGFWEAWTMLTAIAASTTRVQLGTTVLCAGFRNPALLAKMAETLDEVSG
                     GRLILGLGAGWHEPEFDAFGMPFDHRVSRFEEALQVIRPLLHERKVDFRGTYHRAELC
                     EVRPHGPREGGVPILVAAFAPRMLRLTARYADQWTTDWLGPVSKVKEEIGKVRAACEA
                     EGRDPATLTITGGVTVAYPDLGNLPAWMSTSDSYLTGDAEGLAAQLISYRDLGVDHIL
                     TNLYPFSPDSITQYGEAVSIAQKLTR"
     gene            complement(2393..3970)
                     /locus_tag="VGR29_03715"
     CDS             complement(2393..3970)
                     /locus_tag="VGR29_03715"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV2072728.1"
                     /translation="MEPAPPKTHLAVLLLTLLVIVSPFMGGIIVSAQTPLASPVTASA
                     YDPDDLLAIKPGLREEIAAALPPGMTVYDIALTFPEDTSDRTLEGSQEIVYTNTTGEV
                     LNALPFRLYANSADPENNAVTIDEVSVGGAEVSVELSVDNSVAMVALPAPLAPGEEAR
                     IDMGFTTHVPQDEKLHYGIFNHASDTGTWSLAHWYPVVAGRDRETGWMLEPTSEYGDP
                     IFTETGLYTVTVTAPENLTFITSGVEIGSEAAGGIATTTFNASPSRDFVMFADADMKS
                     VAREVSGTTVTSWYEPGHGEAGKAALTWSAQALRLFNNLLGEYPYRQLQLSEIEIYNA
                     AGVEFPQLIALSRGYYQTEPRLESPGYFEFTVAHEVVHEWFYNLVGNNQYAHAFIDEG
                     LTNYLSARVYFEEFYGEQEADQVVRDFLVRPFRNAVVSGVDPVVDFPTDEFPSQQDYV
                     VAAYSKGALGFSAIHEAIGDDAFFDALQVYVQDFRFRVATPEDLLAVFKAVSNIPIEP
                     IWNYWFKERNGDLDAIG"
     gene            4205..4684
                     /locus_tag="VGR29_03720"
     CDS             4205..4684
                     /locus_tag="VGR29_03720"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEV2072729.1"
                     /translation="MQPTVPPSAVERIYLPKSIRDAIMVHLLEAAPNEGVGLLAVDAA
                     YTDEDGDLAIDAVRFFPGTNIESSPSRFTMEPAEVVAALGEMRASDLQFGAIVHSHVR
                     GPATPSATDLQEAYYPDALLMIASFAGQPAVLRAWRIVLEEELQVVQPVDIVVLDGA"
     gene            4760..>4947
                     /locus_tag="VGR29_03725"
     CDS             4760..>4947
                     /locus_tag="VGR29_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072730.1"
                     /translation="MREYQKVPFLAAADASGEPVEMSQDGRSPEVIVDALESMVVALK
                     RLISDRSADELQQAARDGG"
BASE COUNT          973 a         1425 c         1586 g          963 t
ORIGIN      
        1 gatgacgctg ccgatatcct gtggttcgcc tgcgatctcc acaccgagtt cccggagcca
       61 gtcgattgac gcggcaatgt cgttggtcag gaagaggcag agtggctggg cggaatgtcc
      121 cgtgattggc ttgtttgcat ccagaatgag ccctgtttca cctgccatcg ggacatcgta
      181 gatcccacct tggtgcgagg tctggccctc ttcgagccct aacaaccgac tgtaccaggc
      241 aacggcacgc ggcatatcgc tcaccgggac aaagacattg ccgatccggt tctggatggg
      301 gctgcctgtc gaaagcatga tctctccttc aagctgacat acgcaaaacc ggacgcaagt
      361 ctacaatcga tcggccgagg gctcagccgt ttctgggctt tcgtccgcag gccagcagcc
      421 gcaggttgca caatgacgct tgccgatagg gacaccacca ccgcaggatc ggcatccgta
      481 ttccccactc gtgggttccg ggacggtggc tccaagggca aggaatcgaa ggtagagccc
      541 acgatgggcg tcgtttagcc agtggacacc gttggcgtcg agcctcaggc gcacgtcggg
      601 accgacccgt agcgctaaca ggctttccag tgcgcacatg ccactgccga actggagatc
      661 tgtcttcggt cgccatgcga tctcccgcgg cagccagcgc tccgccacct tccgaagcag
      721 gaccttgccc cacgggcgat tggtcgcctt cataatttgg gggtcgaact ggtccatgaa
      781 ggacggcgga agggggtgat ccgtggggga acgcatctgg gagagatcgt cccaagtgca
      841 tgattgggca atatcgagca cctctggttc cagatacgga aagcagggat cccatccgga
      901 agccatgccg atcgatcgtg caggcggatc gatgtacggg atgcgctccc tgacgtacgt
      961 cggccaatcc ccctgatgcc gcaggaaccg gtagccaccg aagaggccgt ccccgtcatc
     1021 accagtggcg aagctggttc catcgaaacc ttttgccgat tccaggcccg cgatcagcgg
     1081 gatgttgttg agctggccga gatcgaagga gcgttgctgc aggatcagcc agtcgagcga
     1141 ttggagggct tcctgctcgg acaggatcac cggcgtccag tcgagatcaa gcgcgtcagc
     1201 taccttttct gcccaagcga tgtccgaatt acagggatag ggaagggatc tgtgttcccg
     1261 gctgcaccgg gtcgatggat ctagcccaac ggtgaccgcc cggaatcgat gaccggcacg
     1321 tttccacatc gccgcaagca gggaggagtc cagcccgcca gagagcagca ggatgttggg
     1381 tcggacccgt tcggtcgcgg tacgcacggc agaatcgagc gcctcgacga gggcgggtga
     1441 cgtatcgttc atgcaggaaa ttgtagtggg acggtgaagg agcatgcgtg gatatcgggt
     1501 tgatcttgcc gctggcggaa gacgaatcgg ggacggcccc atcgtgggcg gagctgagag
     1561 cggcggctgt ccaggcagag gggctgggct tcgactcggt ctgggtctac gatcatctgc
     1621 tccatcgctt cgagggccat cccaccgtcg ggttctggga agcctggacg atgctgacgg
     1681 cgattgccgc atccacaact cgcgtgcagc tcggcaccac cgtgctctgc gccggctttc
     1741 gcaacccggc gctgctcgcg aagatggcgg agaccctcga cgaggtctcc gggggacgac
     1801 tcatcctcgg gttgggagcc ggctggcatg agccggagtt cgacgccttc ggcatgccgt
     1861 ttgatcatcg tgtctcacgt ttcgaagagg cgctccaggt catccgcccg ctgctgcacg
     1921 agcggaaggt cgatttccgg ggaacatacc atcgggcgga gttgtgcgag gtccggccgc
     1981 acggaccccg cgaaggcgga gtaccaatcc tggtggcagc atttgccccg cggatgctgc
     2041 ggctgaccgc gcgctacgcc gatcaatgga cgaccgattg gcttggaccg gtatccaaag
     2101 tgaaagagga gatcgggaag gttcgtgcgg catgcgaggc ggaaggcagg gaccctgcca
     2161 cgcttacgat tacaggcggg gtgacggtcg cgtatcccga tctgggaaac ttgccggcgt
     2221 ggatgtccac gtcagacagc tatctgactg gtgacgcgga gggcctggcc gcgcagctaa
     2281 taagttatcg tgatctgggg gtagaccaca tactgacgaa cctctatccg ttttcgcccg
     2341 attccattac acaatacggt gaagccgtaa gcattgccca aaagctgacg cgttagccga
     2401 ttgcatccag gtcgccgttt ctctccttga accaatagtt ccagattggc tcgatgggaa
     2461 tatttgatac tgccttgaat acggcaagaa gatcctccgg agtggcgacg cggaaccgga
     2521 agtcctgcac gtagacctgc agggcgtcaa agaacgcgtc gtcaccgatc gcctcgtgga
     2581 tcgctgaaaa accgagtgct cccttgctgt aggcagcgac gacataatcc tgctgcgagg
     2641 ggaactcgtc agttggaaag tcgacaacag gatcgacgcc cgagaccacc gcattgcgga
     2701 atggccgaac caggaagtcg cgcacgacct gatcagcctc ctgctccccg tagaattcct
     2761 cgaagtagac acgggcggag agataatttg tcaggccctc gtcgatgaac gcgtgcgcgt
     2821 actgattgtt gccgaccagg ttgtagaacc actcgtgcac gacctcgtgg gcgaccgtga
     2881 attcgaagta tcccgggctc tccaggcgag gctccgtctg gtaatagccc cggctgaggg
     2941 cgataagctg tggaaactcc acaccggcgg cgttgtagat ctcgatttca gaaagttgaa
     3001 gctgccggta ggggtactct cccagcaggt tgttgaagag tcggagcgct tgcgcgctcc
     3061 aggtcaatgc ggctttgccc gcctcgccgt gccccggctc gtaccaggat gtgacggtgg
     3121 taccgctgac ctcccgggcg acagatttca tatcggcatc ggcaaacatc acaaaatcac
     3181 ggctgggcga ggcattgaag gtcgttgtgg ctatgcctcc ggccgcttcg gacccgatct
     3241 caacgccact ggtgatgaaa gtaagattct cgggcgcggt cacagtgacg gtgtaaaggc
     3301 ccgtctctgt aaagataggg tcgccatatt cactggtcgg ttccagcatc cagccagtct
     3361 cccggtcgcg gccggcgacc accgggtacc agtgcgcgag cgaccaggtg ccagtatcgc
     3421 tcgcatggtt gaagatgccg taatggagct tctcatcctg gggcacatgg gtggtgaacc
     3481 ccatgtcgat ccgggcttcc tcacccggtg caagtggcgc tggcaaggcc accatcgcga
     3541 ccgagttgtc gaccgagagc tcaacgctaa cttctgcgcc gccgacgctg acctcatcga
     3601 tggtgacagc gttgttctct ggatcggcac tgttggcata gagccggaag ggaagtgcgt
     3661 tcagcacctc gccggtcgtg ttggtataga caatctcctg tgagccctcc agggttctgt
     3721 cgctggtatc ttcggggaag gtgagggcga tgtcatagac cgtcatccct gggggtagcg
     3781 ccgcggcgat ctcttcccgc aaccccggct tgatcgccag gagatcgtcc gggtcgtagg
     3841 ccgatgcagt gaccggtgag gcaagcggcg tttgggccga gacgatgatc ccacccataa
     3901 atgggctgac gatcacaagc agtgtcagca gcagaacggc aagatgagtc ttgggtgggg
     3961 ctggttccat taaacccgta ctcctgtcac aacgagcgcg ggcaggttct cgcgccggtc
     4021 tctccatatg actgtattgg tacaatccgc cggtgaatcc tcgttcggtg caggtcatgc
     4081 cggcgagata aggtgcaggg aagggagttc ccaccctcga tagtgtaggc gcaacccggc
     4141 agcgggggcc gaatagacgg gcgggaagcc aacgcgctga gtacgtcccg ggagcgatac
     4201 ggcaatgcag cccacagtgc caccttcagc ggtggaacgg atataccttc cgaagtcgat
     4261 tcgtgatgcc attatggtgc acctgctgga ggcagctccc aacgaagggg tcggcctcct
     4321 ggccgttgac gcggcgtata ccgacgagga cggtgacctg gccatagatg cggtacggtt
     4381 cttccccggg acgaacatcg agtcgtctcc gtcgcgcttc actatggaac cggccgaagt
     4441 ggtcgctgcc ctgggtgaga tgcgtgcatc agacctccag ttcggcgcga tcgtgcattc
     4501 gcatgttcgt ggtcccgcga caccttccgc cacggacctg caggaggcgt attatcctga
     4561 cgccttgttg atgattgctt ctttcgcggg tcaaccggct gtcctgcgcg catggcgcat
     4621 cgtcctggag gaagagctgc aggtcgttca gccggtcgat atcgttgtcc tggacggtgc
     4681 atgacgatgc ccgggcctga tcctcgtccg gccggagggc aggcagggat gagccggatc
     4741 aagtggaaaa caggagttca tgagagagta tcaaaaggta ccgttcctgg cagctgcgga
     4801 tgcgtccggt gaaccggtgg agatgtcgca agatgggcga tcgccagagg ttatcgtcga
     4861 cgccctcgaa tccatggtcg ttgccctgaa gcggctcatc tccgatcggt cagccgatga
     4921 gctgcagcaa gctgcgcggg acggagg
//
LOCUS       DASYWM010000093         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_453777, whole genome shotgun sequence.
ACCESSION   DASYWM010000093 DASYWM010000000
VERSION     DASYWM010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453777"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VGR29_03730"
     CDS             complement(<1..600)
                     /locus_tag="VGR29_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072731.1"
                     /translation="MVIRMVIMAMVLGSALVGANGVVAQRSGDDAQVDTTGLGIRKYL
                     CPSAAAFESGSADCELGPGVTFEVATEDGEVLGSCTTEISVIQTSEVATCYVLDVPYD
                     TLLIITEDTSTGPAGYVPLRNPQTMTIERRPDAQDYAPTAVFVNIPAETGGDGEETEA
                     TTEEKTVTTTKVVTLPNTGAGSAAEGGHGTVLLGAAMLLS"
     gene            complement(1088..2089)
                     /locus_tag="VGR29_03735"
     CDS             complement(1088..2089)
                     /locus_tag="VGR29_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV2072732.1"
                     /translation="MSQPKITDTALDQYALTPPVSLFPLLSQGNSNTTIGVRTGASDF
                     ILKIYSGYDDPSRLKSEHELLAWLQQQRLSFAVPAPLRTKAGATMAFSGLGWQALTPF
                     FPGSRVDPEHPRELESFGGALSELLSAFERYPLEVAYVPASEFHIIVPRLPDLQIAVS
                     DLGLEPNRHNDALTDWLRHELAELTTFMKSSYQVLPRQLTHNDFIPSNVLYDHGRIGA
                     VLDFEFVIPGIRGLDFAMALLFCLRYWSNPDPHETTTLLSRGYGRHNSLTAEEVQQLP
                     LLLRLRNMASTLWWLGRALKNGDARSEAIRIERTREFTEWLGTYESRLTAAVASILL"
     gene            complement(3222..>3782)
                     /locus_tag="VGR29_03740"
     CDS             complement(3222..>3782)
                     /locus_tag="VGR29_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072733.1"
                     /translation="VLHGLGLGARQWRCDEYRRLVAIGASLSLDQAIDAYLDLSLAML
                     TFVRPSVYAALELGVQGLQEMLHPGWSHNVADLTQTIEVFAPATSVTDREALGIAMRS
                     LAYGFLFDVKADPLEWRNHARLLIDGCLLRSFHDKGRCRSANLPFDEVRGSGNGLIAT
                     GNIDEGDANGTIIVGPFDAHAVTPTG"
BASE COUNT          785 a         1142 c         1096 g          759 t
ORIGIN      
        1 gctcagcagc atcgctgccc caagcaggac agtgccgtgc ccgccctcgg ccgcactgcc
       61 agctcccgtg ttcggcagcg tgaccacctt ggtcgtggtc acggttttct cttcggtggt
      121 ggcttcggtt tcctcgccgt cgcccccggt ttcggccggg atattgacga agaccgccgt
      181 gggggcgtag tcctgggcat cgggtcgtcg ctcgatcgtc attgtctgcg ggttccgtag
      241 cggcacatag cccgccggac cggtcgaggt gtcctcggtg atgatcagca gcgtgtcgta
      301 gggcacatcc agcacatagc aggtcgccac ctctgaggtc tggatcacgc tgatctcggt
      361 ggtgcagctg cccaggacct ctccgtcctc ggtggcaacc tcgaaggtga caccgggacc
      421 cagctcacag tcggcggagc cgctctcgaa ggctgcggca ctgggacaca ggtacttccg
      481 aatgcccaga ccagtcgtgt ccacctgcgc atcgtcacca gatcgctggg ccaccacccc
      541 gttggccccg accagtgcac tgccaagaac catcgccatg atcaccatgc gaatcaccaa
      601 agaacatcca tgcattgaaa ggcccttccg aataaaatct ctcctctccc gaggtgcggc
      661 cctcgccacc ccttgatgct accgaagacc gcgccgcgtg ccatcgctgg aattaattaa
      721 tcacacgagt taatcactgc gaaccagaca cacaacaacg actcccacgg agagcaggag
      781 cggggcttct ttgcacgcga gaaccctccg ggctgcatgt ggtcacggaa tacagtgccc
      841 gttaatcggt ctcgcgtgtt catcccacgc tgcgtcacca actggtcttc ctggttagtc
      901 cagatatccg ccatcgtgaa cgagccggcc atgtactggc accacacgat gctggccgag
      961 agcccctgca tcgtcggtgt cggaccaccc gaccgagtca gatgcttcac catcacgctg
     1021 gaatgcccga ttgtcgccga ggacgagtag aagtcttgga ggactagtaa ccgacatccg
     1081 tttcagatca cagcaatatg ctggcgacag cagcggtcag tcgtgattca taggtgccaa
     1141 gccactcggt aaactcgcgt gtgcgttcaa ttcgaatggc ctcggaacgc gcatcaccgt
     1201 tcttcaacgc tcgtcccagc caccacagtg tggatgccat gttgcgtagc ctgagcagga
     1261 gcggcaactg ttgaacttcc tcagccgtga gtgagttgtg ccgaccgtag ccccgactga
     1321 gcaacgtcgt cgtttcatga gggtcggggt tggaccagta ccgcagacag aacaagagcg
     1381 ccatggcgaa atcgagcccg cgaatacctg ggatcacaaa ctcaaagtcc aggactgcgc
     1441 cgatacgccc gtgatcgtag aggacattgc tgggaatgaa gtcgttatgc gtcagttgcc
     1501 tgggcaaaac ctggtagctc gacttcatga aagtcgtcaa ctccgccaac tcgtggcgta
     1561 gccagtcggt cagcgcatca ttgtggcggt tgggttcgag tccgaggtct gacacagcga
     1621 tctgaagatc cggcaatcga gggacgatga tgtggaattc actcgctggg acataggcaa
     1681 cctcaagggg ataccgctca aaggcggaga gcagctcact cagagctccg ccaaacgatt
     1741 ccagttcccg gggatgctcc gggtcaaccc gggatccggg aaagaatggc gtgagagcct
     1801 gccagcctaa tccgctgaac gccatggtcg cgcctgcctt tgtcctcaac ggggccggga
     1861 ccgcgaacga tagtcgctgt tgttggagcc acgcgagcaa ctcatgctca ctcttcaaac
     1921 ggcttgggtc gtcgtagccg gagtagatct tcaggatgaa atcggaagcg cctgtccgga
     1981 cccctatggt cgtattgcta ttcccctggc tgagaagcgg gaagagactc actggcggag
     2041 tgagcgcata ctgatccagg gcggtatcgg tgatctttgg ttgggacaca gtgttcctct
     2101 ctgtcaagcg gcagtgacag cggcatcgag cgaagtggtg ccagaaccga tatgggacct
     2161 cggtccagta gcatccgcac cggcatactc tcggatccag tgatcatctc gcgggcctca
     2221 tcgacgctga tcttctcatc cggtggtggc cagttgggga ggcggaactt acgaccatgc
     2281 ctggctccat cgctcgatgg agccaaacag atgtctggga tttcgcgaca tcggcggctg
     2341 ttttcgtctc agggagagac attcattcct cagcagcgga catgccaacg cccttggttg
     2401 gggagtaaga gcactgtcgt gctcgtcgcc tgcgagcgcg ccgaggctgc gggatgtggc
     2461 aaccgttcgg ataccgtcta cgtggtgatc ggggaagaca gcaagaatat tctcccgatc
     2521 acaccgagtg gtgacgggag gcgtggccta aacgtgatca gacgccagaa tcactccggt
     2581 gacgccgacg cctgccgcgt ctcatacccc accacgagat gtgtgtaacc tggggcagtg
     2641 gatggggaat ggatacactg gcctgctcgg tgcccagtcc cgcttgccct catcctccca
     2701 aggaccacat caaggcttcg gttctctacg gcacataacg tgctatcgaa tacacccaaa
     2761 gcatcaggtt ccattcgagg atgaggacac catgactgat cgcactgccc ctccctgcga
     2821 accagctgat accggggaat gtccttggtc atcccaatca gaccttgtga ccgagtctgg
     2881 ccgattctgg aaaggggtga tgattgcttt accgccgggt attgcttttt gggcggcgat
     2941 catctggatc atcctcagct tcaagtagat tcggttgcca gtcatcgaga gcccctgtct
     3001 tccccgccac tacattcggc acccggcagt catgccgcga acgcgactcc tgatacctcg
     3061 ggagtcgttg tcatgtcctt cttcgctcac gcgcgaggaa tcatcggact gcatggggta
     3121 tcggaacaga cagacgtcgg cattcgcgac aagcgtgtgt ccagctaaag cacttttccc
     3181 agtatgtata cggccagcca acaagccttg gggggtggaa attatcccgt cggtgtgact
     3241 gcatgagcgt cgaagggtcc aacgatgatc gtaccgttgg catccccttc atcaatattg
     3301 cccgtcgcaa tcaagccatt ccctgagccg cgcacttcgt cgaatgggag gttggctgat
     3361 cgacagcggc ctttgtcatg gaaagatcgc aacaggcagc catcgatcag caacctggca
     3421 tggttgcgcc actcgagagg gtccgccttt acatcgaaga gaaatccata tgccaggctc
     3481 cgcatcgcga tcccgagcgc ctcacggtcg gtcacagacg tggccggcgc aaacacttcg
     3541 atggtttggg tgagatccgc gacgttgtga ctccaccccg gatgcagcat ctcctgcagt
     3601 ccctggacgc ctaactcaag cgccgcgtag accgatggac gcacaaaggt cagcatggca
     3661 agcgagaggt cgagataggc gtcgatggct tggtcgaggc tcagggacgc accgattgcc
     3721 acaagcctcc ggtactcatc acagcgccat tgccgagccc cgagaccgag gccgtgcagg
     3781 ac
//
LOCUS       DASYWM010000094         6835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_21620, whole genome shotgun sequence.
ACCESSION   DASYWM010000094 DASYWM010000000
VERSION     DASYWM010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21620"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /gene="aroC"
                     /locus_tag="VGR29_03745"
     CDS             <1..988
                     /gene="aroC"
                     /locus_tag="VGR29_03745"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV2072734.1"
                     /translation="PAGIPLTVDEIQHELNRRRVGQSKVTTPRNEKDQAELLSGVYEG
                     ITTGTPISLIAYNADVDSSKYDNLRDVFRPGHADYTYWRKYGHRDHRGGGRSSARETW
                     GRVAAGAIARKILATVGTEVYAFTREIGGIEMETFDRDQIEQNIVRTPDPVAAEKMID
                     AIMAIKEAKDSLGGIVEVRATGVPVGLGEPTFDKLEALIGQAMLSIPATKGVEIGRGF
                     ALARERGSSSNDAFYMEDGNVRIGDNNAGGTLGGISTGEALVVRVAIKPTSSIEQEQD
                     TIDTDLRPRKITVYGRHDPSVVPRAVSVIEAMLAITLADLLLRNRSARIDWP"
     gene            complement(1186..1425)
                     /locus_tag="VGR29_03750"
     CDS             complement(1186..1425)
                     /locus_tag="VGR29_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072735.1"
                     /translation="MSYGSGAGGSPAVEHLRGVMGEPKIYDLREQALLPKYYNRLNDQ
                     GQYQFSDGEVEAANTDLDEFVFWSDQMKSAREKLG"
     gene            2011..3501
                     /locus_tag="VGR29_03755"
     CDS             2011..3501
                     /locus_tag="VGR29_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2072736.1"
                     /translation="MANTAILEQQGSLPVYGNFINGEWTEAKSGGTFENRNPARASEV
                     IGLFQSGGAEDVHAAVTAADDAARAWGEMSAIARANILYTASEILASRVNDVGRDLSC
                     EEGKTLKEGIGETTRAVQILRYYAGEAQQPIGEHYPSTNPTTMLYTLREPLGVVGIIT
                     PWNFPIAIPAWKIVPALAFGNTVVFKPATATPLCAVHLVAALEEAGLPKGVLNLVTGS
                     AGSVGDPLIKDERVLAISFTGSNEVGQEIKRIASDRGAKVQLELGGKNPAIVLADADI
                     DHAVTQVINGAMMSTGQKCTATSRAIVDRKIASELTERLAQQIGGLKVGDPLADGITI
                     GPLIDEKAAERVAAEIGRAKSEGVALVTGGERPEALSDGHFVMPALFSDVDPQSRLGQ
                     EEMFGPVLGVIPVDDVNEAIDVANQVRYGLSASIFTRDLGRAMQFVRQIHAGIVHVNS
                     ETAGAEPHVPFGGYKGSSSYSREQGKAAREFYTQIKTVYLDPPPSA"
     gene            3608..4054
                     /locus_tag="VGR29_03760"
     CDS             3608..4054
                     /locus_tag="VGR29_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEV2072737.1"
                     /translation="MAITERSARTTWQGDLKTGEGRVSSGLGTFTDLPMSLPSRIGEA
                     NGYSSPEDLLAGAHAGCLAMNLSGTLANNGTPAERIDVNAVVGVGPKEGGGLEVKHSH
                     VTMSAKVPNLSEDEFRKLAEQAEQTCPISNAIRGNVEITTEIAFEG"
     gene            complement(4128..5726)
                     /locus_tag="VGR29_03765"
     CDS             complement(4128..5726)
                     /locus_tag="VGR29_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072738.1"
                     /translation="MTAVSQPPAPTTDFRTAEPRLRRWSIAIAQRLQGLTESQLNGMI
                     WLSSLAMCLFLLGSSRTSITGTMNIVENWGAVERVALWQDFLTPVIPRYTLPTGVIAW
                     GFRVAMLIAFLAQAGAFLVVMKSPNPSLGRWLIGPIGAHIIMMFLMVPSNSDVFFYEA
                     VGDLAARGFNPYVHELMDFPDHPLIPWNYWIDIGTVYGPLWVQYSRIVMMVAGPDPVL
                     ATLLQKLLVGLVTFALVGVVYWFAKRLSGRQSLATAAGVLVAWQPNMIIETSGQVHND
                     PHTVLLATIGLALVILGGIAGIRAGIILVTLSMMIKFITIPLLGVLAIVRIVDGHNPN
                     PVRRIIGNWLLDGAAIVAVILAAFLPFWEGYETIEEMLAEPGRLYTHPIWRLIQSAMG
                     FLLPAGASDVWNAISRPFLQAATLALFIGVIVFQGIRLWNDRRAAATAPEEMTRSGNL
                     PWWTRHVLYAWMIMFLVLAYVPVNSHPWYWVWPVAAVGLVIAFDFRSRHESWSVALLP
                     TWFWIYLWGNALLTLMYHTRIARY"
     gene            complement(5835..>6835)
                     /locus_tag="VGR29_03770"
     CDS             complement(5835..>6835)
                     /locus_tag="VGR29_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV2072739.1"
                     /translation="SCDRMLQAAAIIAQIDAAANLAEVAVDRQYVRPVLSNDRSLEIV
                     DGRHPVLETALPAGEYVPNDARLDTETEQIVILTGPNMAGKSSWLRQVALITLMAQIG
                     SFVPATRASIGIVDRIFTRIGAQDDIATGQSTFMVEMLEMANILHHASPRSLVVLDEI
                     GRGTSTYDGLAIARAIVEFVHNSPSLGCKTLFATHYHELTELAGVLPRVSCYRMDVLE
                     DGERVVFLRKVVAGNADKSYGIHVARLAGIPKGITRRAEEILADLEQQHPANEDRSRR
                     RDAMRQPIPGNGPKVQLTFFDAPNPVVERLKSLEVEALTPLDAIRLLYELRQEAGS"
BASE COUNT         1472 a         1955 c         2120 g         1288 t
ORIGIN      
        1 tcctgcagga attcccctga cggtagacga gatccagcat gagcttaatc gccgccgtgt
       61 tggccagagc aaggtcacga ccccacgaaa cgagaaagat caggcagaac tgctctcggg
      121 ggtctatgag ggcatcacaa ccggcacgcc catctcgctg attgcgtaca acgccgacgt
      181 tgactcgtcg aaatacgaca acctgcgtga tgtctttcgg cccggccatg ccgattacac
      241 atattggcgg aagtatggcc accgtgacca tcgtggaggc gggcggtcct cggcccggga
      301 aacatggggc cgggttgcgg ctggggccat cgcgcgaaaa atcctcgcca cagtcggcac
      361 agaggtctac gccttcaccc gggagatcgg cgggatcgag atggagacct tcgatcgcga
      421 ccagatcgag cagaacatcg tccgcacgcc agatcctgtc gctgccgaga agatgatcga
      481 cgcaattatg gcgatcaagg aagcgaaaga tagcctgggc ggtattgtgg aggtccgggc
      541 aactggtgtc ccagtggggc ttggcgaacc cacgttcgac aagctcgaag cactgatcgg
      601 acaagcgatg ttgagcatcc ccgccacaaa gggggtcgag atcggtcgcg gcttcgcact
      661 cgcacgggag cgcggttctt cctcaaacga tgcgttttac atggaggacg gtaacgtccg
      721 catcggggat aacaacgctg gcggaacact cggtggcatc tccaccggtg aagccctcgt
      781 cgtccgcgtc gcaatcaagc ctacctcctc cattgagcag gagcaggata cgatcgacac
      841 cgacctccgg ccgcgaaaga ttaccgtcta cggccgtcat gacccctctg tcgtgcctcg
      901 tgccgtctcc gtgatcgagg caatgcttgc aataaccctc gcggatctcc tgctccggaa
      961 ccggtccgct cgtatcgatt ggccatagcg atatcgtgca attccggtgg cgacagggcg
     1021 ttacctgcta caccgacagg cccgtcgttc cgagctgagc gaagaatgac gggggtgcta
     1081 agtcctgagg aaagagttgt cggaccagaa gccacacgga tatctccact tcgttggaca
     1141 tacggcaccg ttccatccgt ggggtcaatg agcccaactg cactgctagc ccaacttttc
     1201 ccgagctgat ttcatctgat cgctccagaa cacaaactcg tcgaggtccg tatttgcggc
     1261 ctccacttcc ccgtccgaga actggtactg gccctgatcg ttcagcctgt tgtagtactt
     1321 gggcaagagt gcctgctcac gcaggtcata gatcttgggt tcgcccatca cccctcgcag
     1381 atgctcgacg gcgggggacc cgcccgcgcc acttccgtag ctcacaaagt tggaggcctt
     1441 ctagttccat ttctggtaca ggaagtcgat cgcgttcttg agggcgccgg gatcgagtga
     1501 ttgtactcgg cggtgacgaa gacaaggccg tccaggcggc caatgtgctc ggctcgtcgc
     1561 ttcgtgcgct cgttctggta ctgtgtatgg cgggtgggat tggctcatca agaaggggaa
     1621 aactgacctc ctccaaatca acaagctcga actccgcccc gccccgttgt ttggcaagct
     1681 cgaatatcca gttggcgggt tgtatcccaa atcgaccagg tcgcgttgag ccgaggataa
     1741 tcccaatatt gaccataatg tgcctcctgc tgaacgttgt gccaatgcgc gcctgattca
     1801 ggttgggtgg cgtgcgcacc tcaatcaccg gacgacgctg gccggtggcg aaaatcaccc
     1861 tcattccatg tgcccagtgc ggaactcctc gtgacgataa gccggcatcg gaggcgaccc
     1921 gcttgcggtg gaattgcgta tcgacggtga gattggtagc gtacagatga gtgatgacgg
     1981 caacaatgct cgtgaacaga aggagacatc gtggcgaaca cggcaatact tgaacagcag
     2041 ggcagcttgc cggtctacgg caacttcata aatggcgaat ggaccgaggc aaaaagcggc
     2101 ggaacattcg agaaccggaa tcccgcccgg gcatccgaag taatcgggct ctttcagtcc
     2161 ggtggcgcgg aggatgtcca tgcggccgtt acggcggcgg acgatgctgc tcgcgcgtgg
     2221 ggtgaaatgt cggcgattgc ccgcgccaat atcctgtaca cggcaagcga aattctcgcc
     2281 tcacgcgtga atgatgtcgg ccgtgacctg agttgtgaag agggcaagac gctcaaggaa
     2341 gggatcggcg agacaacccg ggcggtgcag atcctgcggt actacgccgg tgaggcgcaa
     2401 cagcccattg gcgagcacta cccgagcacg aaccccacga cgatgttgta tacgcttcgg
     2461 gaaccgctgg gtgtcgtggg aattatcacc ccctggaact ttccgattgc gatcccggcg
     2521 tggaagatcg tgccggcgtt agcgtttggc aacacagtgg tcttcaaacc ggcgacggcg
     2581 actcctctct gcgccgtgca tctcgttgct gcgcttgaag aggctggact gccaaagggg
     2641 gtgctaaacc tggtgacggg atccgcaggg tccgttggtg atcctctcat caaggacgaa
     2701 cgggtactag cgatctcgtt caccggttcc aatgaggtcg gccaggagat caagagaatt
     2761 gcatcagatc gcggcgcgaa ggtgcagctt gagcttggag gtaagaatcc ggcgatcgtc
     2821 cttgctgatg cagacattga tcatgccgtc acgcaggtga tcaatggcgc aatgatgagt
     2881 accggccaga aatgcaccgc gacgagtcgt gcgatcgtgg atcgcaagat cgcctcggaa
     2941 ctcaccgaac gcttggcaca gcagatcggt ggcctgaagg tcggggatcc gctggcagat
     3001 ggaataacta ttggaccgct gatcgacgag aaggcggccg agcgggtcgc cgcggagatc
     3061 ggcagggcga agagcgaagg tgtggcgctt gtcaccgggg gtgagcgtcc ggaagcgctg
     3121 tcagacggcc atttcgtgat gccggccctc ttctcggatg tcgatccaca gtcccgcctc
     3181 ggccaggagg agatgttcgg gccggtgctg ggcgtgatcc cggtggacga tgtcaatgag
     3241 gccattgacg ttgcgaacca ggtgcgatat ggactatcgg cctcgatctt cacgcgcgac
     3301 ctcgggcgtg cgatgcagtt cgttcgacag attcatgccg gtatcgtgca cgtgaattcc
     3361 gaaacggcag gagctgagcc gcacgtgcca ttcggcggct acaagggctc cagctcctac
     3421 tcgcgggagc agggcaaggc cgcacgggag ttctacacgc agatcaagac ggtgtatctc
     3481 gatcccccgc cgtcggcctg acgcgacgga tcgcgcagca tgaggttcgt ggcttggaac
     3541 tggcggtatc ttgagcagga acaccatgac cccattgtgg atcgttgcaa cgagaaggag
     3601 taatgacatg gcaatcacag agcgaagtgc ccgtacgact tggcaaggag acctcaagac
     3661 cggcgaaggc agggtctctt ccgggcttgg taccttcacc gatctcccga tgtctctacc
     3721 gtcgcgaata ggggaggcaa atggctactc gagcccggag gatctgcttg cgggcgctca
     3781 cgcgggctgc ctggctatga acctttcggg gacattggcc aacaacggca caccggccga
     3841 acgaatcgat gtcaacgcag tcgtcggagt cggaccgaag gaagggggcg ggctggaggt
     3901 caagcattcc cacgtgacga tgagtgccaa ggtgccaaac ctttccgagg acgagttccg
     3961 caagctcgct gagcaggcgg agcagacctg cccgatttcg aacgcgatcc gtggcaacgt
     4021 cgagatcacc acggagatcg cgttcgaagg ctaatgcatt agtccagctg agatgcaaag
     4081 gggtagccaa tgtacatcgg ctaccccttt gcctgtcggt agcggcccta gtagcgggca
     4141 atccgggtgt gatacatcag ggtcaggagc gcattgcccc agaggtatat ccagaaccag
     4201 gtaggaagca gggcgacgct ccaggattca tgtctgctcc tgaaatcaaa ggcgatcacc
     4261 aggcccaccg ccgccaccgg ccatacccaa taccaggggt gggagttcac tggaacgtac
     4321 gccagcacga ggaacatgat catccaggca tagagcacgt gacgggtcca ccagggcagg
     4381 ttgccggatc tcgtcatctc ttcgggtgct gtggcggcgg ctctcctgtc gttccaaagc
     4441 ctgatccctt gaaagacaat gacaccgata aagagagcca gcgtcgccgc ctggaggaag
     4501 ggacgcgata tcgcgttcca gacgtccgaa gcaccagcag gcagcaggaa gcccatcgcc
     4561 gactggatga gccgccagat cggatgggtg tacagccggc caggctccgc gagcatctcc
     4621 tcgatcgtct cgtatccctc ccaaaaaggc aggaatgccg cgagaatgac cgccacgatg
     4681 gcggcaccat cgaggagcca gttgccgatg atgcggcgaa ccgggttcgg attgtgaccg
     4741 tcgacgatcc gcacgatggc gagcacgccg aggaggggga tcgtgatgaa cttgatcatc
     4801 atcgacaagg tgacaaggat gattcccgcg cgtatcccgg cgatcccacc gagaatgacg
     4861 agcgcgagcc caatggtggc caagaggaca gtatgcggat cgttgtgaac ctgtccggag
     4921 gtttcgatga tcatgttggg ttgccacgcc acgagcaccc cggcggcggt ggcaagtgac
     4981 tggcggccgc tgagccgttt cgcaaaccag tagaccactc cgaccagggc gaaggtgacc
     5041 aatccgacca ggagcttctg cagcagggtg gcgaggacag gatcgggacc ggcgaccatc
     5101 atcacgatgc ggctgtactg cacccagaga ggcccataga cggtgccgat atcgatccag
     5161 tagttccatg ggataagggg atgatcgggg aagtccatga gctcatggac atacgggttg
     5221 aaaccacgcg ctgcaaggtc gcctacagct tcatagaaaa agacatctga gtttgacggc
     5281 accatcagga acatcatgat gatgtgggca ccaattggtc cgatcaacca gcgcccgagt
     5341 gaagggttcg gcgatttcat cacgacgaga aatgcaccgg cctgggccag gaacgcgatg
     5401 agcatcgcca ccctgaaacc ccaggctatg acaccggtgg gtagggtgta gcggggaatg
     5461 acaggtgtaa ggaagtcctg ccagagcgcc acccgctcga ccgcacccca gttctccacg
     5521 atgttcatcg tgccggtgat gctggtcctc gacgatccaa ggaggaacag gcacatcgca
     5581 agcgaggaga gccagatcat gccgttgagc tggctctccg tcagtccctg gagtctctgc
     5641 gcaatggcaa tagaccagcg tctcaatcgg ggctcggcgg tcctgaaatc agttgtcggc
     5701 gctggtggtt gcgataccgc cgtcatgaga acctttgagg catggaagga agtgcatcct
     5761 tagtcagcag tgttcgggac cgctggagac cgaacagcag ccctgccacg ttgtttgtag
     5821 catgttctgc atggttacga gccagcctct tgccgcaact cgtacaggag ccgaattgcg
     5881 tcgagcggag tcagtgcctc gacctcaagc gatttaaggc gttctacgac aggattcggc
     5941 gcatcgaaga atgttagctg cacctttggc ccgttcccgg gaatcggctg tcgcattgca
     6001 tcacggcggc gcgaccggtc ctcgttcgcg ggatgttgct gttcgaggtc agccagaatt
     6061 tcctctgccc gccgggtaat tcccttcggg ataccggcca gcctcgccac atggatgccg
     6121 tatgacttgt ccgcattacc agccacgacc ttgcgcagga agaccacacg ctcaccatct
     6181 tcaagcacat ccatgcgata gcaggacacg cgcggcaaca ccccggcgag ctccgtgagc
     6241 tcatgatagt gggtcgcgaa gagcgtcttg cagccgaggg atggcgaatt atggacgaac
     6301 tccacgatag cgcgtgctat ggcgaggcca tcgtaggtgc tggtcccgcg tccaatctcg
     6361 tcgagaacca cgagggatcg cgggctggcg tggtggagga tgttcgccat ctccagcatt
     6421 tccaccatga aggtgctttg gccggtggca atatcgtcct gcgcgccaat ccttgtaaaa
     6481 atccggtcaa caatcccgat cgatgctcgt gtggctggca cgaaggagcc aatctgcgcc
     6541 atgagcgtga tcagcgccac ctgccgcagc cagctcgatt taccggccat gtttggtccg
     6601 gtaaggatga cgatctgttc ggtctccgtg tcgaggcgtg catcattggg gacatactct
     6661 ccggcaggga gggctgtctc caaaacgggg tgccgcccgt ccacaatctc cagagaacga
     6721 tcgttactga ggacaggacg aacatactgc cgatcgactg cgacctctgc caggttggcc
     6781 gccgcgtcga tctgcgcgat gattgccgct gcctgtagca tgcggtcaca ggaac
//
LOCUS       DASYWM010000095         5672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_416137, whole genome shotgun sequence.
ACCESSION   DASYWM010000095 DASYWM010000000
VERSION     DASYWM010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416137"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VGR29_03775"
     CDS             complement(<1..335)
                     /locus_tag="VGR29_03775"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2072740.1"
                     /translation="MNIGKRRSRRYYGWTITWTFALTETVSWGILQYAFSALLVPMQE
                     DLGWSAAAITGAYSLSMLVTGLAAPFVGRWIDAHGPRALMTAGSVAGTLLVLAWSRVE
                     TLTGFYLLWV"
     gene            complement(658..1740)
                     /locus_tag="VGR29_03780"
     CDS             complement(658..1740)
                     /locus_tag="VGR29_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEV2072741.1"
                     /translation="MTTRPGRRTNERVRVLRPYEASLQREEMNAGIDEDQVLIRCCYS
                     AISPGTELGLFTGSHGGIVDPGHGPAKEDPFFPGFKFPFSPGYAAVGEVEMAGTHVHD
                     VSKGDIVYYPGRHQRYSIVSPALTPVIPVPSSMSLLAAPFARFGQIASTALVFSEAGE
                     GDLVAVIGLGLIGNLAAQLFRIHGASVIGADFVGFRRVLARQAGIHHTIEAESGYTVS
                     RIMDASEGNGARTVIEATGNPRLVESALQMAAPKGEVILLGSPLTASGHDEALSVYVL
                     HLIHRKGVRITGAHESLIPVIAAHGDILDQRTLARKALDLIDRKHLVVEHLISDIVRP
                     GEIERAYTSLLRDKDTTMAVLIDWTG"
     gene            2091..2687
                     /locus_tag="VGR29_03785"
     CDS             2091..2687
                     /locus_tag="VGR29_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072742.1"
                     /translation="MVIRCVRVLMLLALLFAGFFSLTIMVSASAAMPHNEYMALLQDD
                     PLLPGDLTPDDDGDGVVDENDSAPNDPNEGTAPEPGDTDPIADGDNDGLPNALDPDDD
                     SDGVSDDDENLGGDNAPSEPVTKPTSPSGPRSDPAPEPEPVFADAVPARTTDDHPVVL
                     AFPRPGVVAPLTACWLSGCSPAPAFSFTAGGSLLIRRR"
     gene            2774..3250
                     /locus_tag="VGR29_03790"
     CDS             2774..3250
                     /locus_tag="VGR29_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072743.1"
                     /translation="MTPEPDAASPPPSRSFRLRDGGIATFFLIAALLILLGLTRTDVI
                     AYAQQIDLFYITVMLIALAVPGPIIRPWDLPGQLLLWNAAGWCLGLVILGMASFGASP
                     MLPLILIAFALSFWPREDSVPLPFPWMGASIGLVGGFLICWALWDNAYVDIPFSNI"
     gene            3415..4200
                     /locus_tag="VGR29_03795"
     CDS             3415..4200
                     /locus_tag="VGR29_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEV2072744.1"
                     /translation="MSPTALNTYRNCAYRVRLAHIDRVPEPPGYHLFLRKGRIAHNLL
                     RDIAHLLKRRYPMIDEDEILKRARLRLPPQVFPSEAERELHAKEIVRWVLYGSRYLAR
                     VPDPTWLPIENNQTRRWSVYPGRAPYTIMARPDVVLQHIDEEDQPLIEIIDYKTGKIR
                     LEDDPPVLMRFVIRDLLARAVGNASSAQVRFTWLWLDSARTTQIDLSVKHCNEHWSRI
                     TQHLQDLASETAWKATPSFLCRYCPYHRNAGTTEIPLEDPGYS"
     gene            4274..4825
                     /locus_tag="VGR29_03800"
     CDS             4274..4825
                     /locus_tag="VGR29_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV2072745.1"
                     /translation="MVEITSNADALEAVRTVRQVRQYEEGTVSPDDLNQVLEIARWTG
                     SSRNTQPWHFVVVDDKGMLKQLSDLRENITWVADGALAIAILLPNENPPHEAYDAGRV
                     TERIMIAARFLGLGSGTAWFGEPEREAKAKQLLGVPEHMSCHSVVVIGPYVTSRDPRP
                     TGPKPGRKSLDELVSYGTYGETR"
     gene            5003..5287
                     /locus_tag="VGR29_03805"
     CDS             5003..5287
                     /locus_tag="VGR29_03805"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV2072746.1"
                     /translation="MPRKFRDKRTAQFAAGMRVRAFSGFERQADKRLTALMAAETLND
                     LRVNRGNRQEQLSGDRNGQWSIRINDQWRICFEGTEDTIDPTEIEIVDYH"
     gene            5296..>5672
                     /locus_tag="VGR29_03810"
     CDS             5296..>5672
                     /locus_tag="VGR29_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEV2072747.1"
                     /translation="MTAHRPIHAIHPGEYLLEEIEARDMTAANLSRALGVPQNRISEI
                     LRGRRAITVDTALRLARWIGSSPQYWLNLQQTYDLRVAEQALWPEIERTVTPDSSPIH
                     AAWPAFVTGPATNSNREGAGAEVG"
BASE COUNT         1183 a         1756 c         1640 g         1093 t
ORIGIN      
        1 acccagagta gatagaaacc ggtgagcgtc tcgacgcggg accatgcaag gaccaacagg
       61 gttccggcga cggatccggc cgtcatcaat gcacgcgggc cgtgggcatc gatccagcgc
      121 ccgacaaagg gggccgcaag acccgtaacc agcatcgaga gcgagtacgc acccgtgatc
      181 gcagccgccg accagcccaa atcttcctgc atcggaacca gcagggcgga gaaggcatat
      241 tgaaggatgc cccatgagac cgtctcggtc agcgcaaagg tccaggtgat cgtccagccg
      301 taatagcgac gggagcgtcg tttaccgatg ttcatctcat ggtgctgtcc cggctcgtgc
      361 gcttggtgca acgcaggaca tcgtgcggaa gaacatggcc tgcgtcaaga gacgttcttc
      421 gatcacgtgc ctgagcggaa gcaggttgga atggtagtca tgggccccga cgaggtacgc
      481 cacatgcttc ccgcccgagc actcggaaac actcgcgtat tgctcctacc gtctcatgac
      541 gggctctttc gcgacggcga aggtcctgcg agatccggaa caatcgcgga ataatcccac
      601 cacacaactc cccgatgtca tcgacgatgc ccgtcaggta catgtcgccg gtccttacta
      661 ccccgtccag tcgatcagga cggccatcgt tgtgtccttg tccctgagaa gcgatgtata
      721 cgcccgttcg atttcacctg gtctgacaat atctgaaata aggtgctcca ccaccaggtg
      781 cttcctgtca atgagatcta atgcttttcg cgccagtgtc cgctggtcga gtatgtcgcc
      841 atgagcagca atgacaggaa tgagtgactc gtgagcacct gtgatccgca ctccttttcg
      901 atgaatgagg tggagcacgt agacgctgag tgcctcgtcg tggcctgagg cggtcaacgg
      961 tgaacctaaa agaatcactt cgcctttcgg cgccgccatc tgcagggcag actcaacgag
     1021 cctagggttg cccgtcgcct caatgaccgt ccgggcaccg ttaccttcag aagcatccat
     1081 gatgcggctc acggtatacc ccgattccgc ctcgatcgtg tggtgaatac cggcttgcct
     1141 ggcgaggacc cggcgaaaac cgacgaaatc cgcgccaatc acggatgcac catggatgcg
     1201 aaagagttgc gccgccaggt tgccgatcag gccgagcccg atcacggcaa cgagatcccc
     1261 ttctcccgcc tcggagaaca ccagagcggt acttgcgatt tggccaaacc gggcaaaggg
     1321 tgcagctagc agcgacatgc ttgagggcac cggtatcacg ggtgttagcg caggcgaaac
     1381 gatcgaatag cgttgatgcc gccccggata gtagacgata tctcccttgg agacatcatg
     1441 gacatgagtt cctgccatct ctacttcacc gactgccgca taacctggtg agaagggaaa
     1501 tttaaacccg ggaaagaacg gatcctcctt cgccgggccg tggccgggat caacgatacc
     1561 gccatgcgaa ccggtaaaga gcccgagctc ggtgcctgga ctgatggcgc tgtagcagca
     1621 tcggatcagc acctggtcct cgtcgatccc ggcgttcatc tcttcgcgtt gaagtgaggc
     1681 ttcgtatggc cggagtaccc ggacccgctc gttcgttcgc cttcccggtc gcgtcgtcat
     1741 gaccctggct ccttgtggat tctcttcctg atgctccccg cgtggacgac cagagcgcca
     1801 tgtacctgag cgacccgcga cagcggcgta tggtcaggga catacatcgt acgacgaccc
     1861 caggctcatc ggagcccctg gcacaggaga caagtgggtt gctccctaaa gacgcaaaaa
     1921 atgaccagaa aaacctcttg acaggcaatg ttcgtcagtg gacgatctgc acaggtttac
     1981 caacattcag aaacgcgcgt ttctttcgca acacgcagtg ttccggcgct atcgatcggg
     2041 ttcctttcgg caggatcact tcgtcctgtt cgtctcatgg aggttgtctg atggtgattc
     2101 gttgcgtgcg cgtgctgatg ctcctggcac ttctgtttgc aggtttcttc tccctcacga
     2161 tcatggtgag cgcctctgcg gcgatgcccc acaacgaata tatggcgctg ctccaagacg
     2221 atcctctgct cccaggtgac ctgacgcccg acgacgatgg cgacggggtc gtcgacgaga
     2281 atgactccgc gccgaacgac ccgaacgagg gcacggcgcc ggagcccggc gatacagacc
     2341 cgatagcgga cggcgataac gatggactgc cgaacgctct ggaccccgac gatgatagtg
     2401 atggcgtgag cgacgatgac gagaacctgg gcggtgacaa cgccccttca gagccggtga
     2461 ccaagccgac ctcaccgtcg ggaccccgct ccgaccccgc gcctgagcct gaaccggtct
     2521 ttgccgatgc agtgcccgct cgcacgacag atgatcaccc tgtggtgctc gcattccctc
     2581 gaccgggagt ggtggcgcca ctgaccgcat gctggttatc gggatgctca ccggcaccag
     2641 ccttctcctt caccgcgggt gggtccctac tgatcaggcg acgctagtgc gacatcgccc
     2701 ctctttcgtc agccgtcatc tgggatactg aacgctgata agtccggagg ctgcacgagg
     2761 aggggcatga aggatgacac cggagccgga cgccgcgtcc ccacccccct cgcggtcgtt
     2821 ccggctgcgg gatggtggca tcgcgacctt cttcctcatc gcagccctgc tgatcctgct
     2881 ggggcttacc cgcaccgatg tcattgccta cgcccagcag atcgacctct tctacatcac
     2941 cgtgatgctc atcgccctcg cagtcccggg accgatcatc cgcccctggg atctccctgg
     3001 acagctgctg ctctggaatg cagccgggtg gtgcctgggc cttgttatcc tgggcatggc
     3061 ttcgttcggc gcgagtccga tgctgccgct gatcctgatc gccttcgccc tttccttctg
     3121 gccacgggaa gacagtgttc cccttccctt tccctggatg ggagccagca tcggactggt
     3181 aggcgggttc ctcatctgct gggcactctg ggacaacgcc tacgtggata tccccttctc
     3241 caacatctga ttgcccactg gggcttcaca acctccgtca cctcagggac actgaactca
     3301 cagcagatgt gccggaagcg ggtcgcgcat gaaccatcgt cctcaccgag tatcgcaatg
     3361 acggagctgc acgaccctca gacgggcgga ccacgctggc ctcacgatcg gtagatctcc
     3421 cctactgcgc tcaacaccta ccggaattgc gcctatcgtg ttcgcctcgc acacatcgat
     3481 cgagtgccgg agcctcccgg ctatcacctc ttcctccgca aaggcaggat cgcccacaac
     3541 ctattgcggg atatcgcgca tctgctgaaa aggcggtacc ccatgattga cgaagacgag
     3601 atactgaagc gcgcccggct gcgcctccca ccgcaggtct tcccgtccga ggccgaacgg
     3661 gagctccatg cgaaggagat cgtccgttgg gtgctttacg gatcgcgcta tcttgcccgc
     3721 gttccagatc cgacctggct accgatcgag aacaaccaga cgcgcaggtg gtccgtgtat
     3781 cccggacgtg caccctacac catcatggcg cgacctgatg tcgtgctgca gcacatcgac
     3841 gaggaggacc agccgctcat cgagatcatt gattacaaga cggggaagat ccgcctggaa
     3901 gacgatcctc cggtgctcat gcgcttcgtc atccgcgacc tgctcgcgcg tgccgtgggc
     3961 aatgcctcat cagcacaggt ccgcttcacc tggctgtggc tcgatagcgc caggacgacg
     4021 cagattgacc tgagcgtgaa gcactgcaac gaacactgga gcaggatcac gcaacatctg
     4081 caggaccttg caagtgagac ggcatggaaa gcaacaccct catttctctg caggtactgc
     4141 ccgtatcacc ggaacgcagg cacaacagag atcccgttag aagatccagg atattcctga
     4201 tccccacgca gtgatctcca gcggtatgat gcacgcagag cgatagagcc catatacccc
     4261 aggaggtcag atcatggttg agatcacgag caacgccgat gcgctggaag cggttcgtac
     4321 cgtccggcag gtccgccaat atgaagaggg cacggtctcc cctgacgacc tgaaccaggt
     4381 tctggagatc gcccgctgga ccggtagctc ccggaacacg cagccatggc actttgtcgt
     4441 ggtggacgac aaggggatgc tgaagcagct ctcggatctg cgggagaata tcacctgggt
     4501 tgcggatggc gccctcgcca tcgcgatcct cctgccaaac gagaacccgc cccacgaggc
     4561 ctatgacgcg ggccgtgtca ccgagcggat catgatcgcc gcccgtttcc tcgggctggg
     4621 atccggcact gcatggttcg gggagccgga gcgtgaggcg aaggccaagc agctgcttgg
     4681 ggtgccggag catatgagct gccactcggt cgtcgtcatc ggcccgtacg tcacctccag
     4741 ggacccacgg ccgaccggtc ccaagccggg acgcaaatcc ctcgacgaac tggttagcta
     4801 cggcacgtac ggtgaaacgc gctgaaatcg cctacgggcg tgtaaccgta gtcggctccg
     4861 cgagattcgc tcggagccga ctacgggaac ttctccggag aaatagataa caggcaggtc
     4921 gcctggtgtt cttaggctgt atctgcgaag tataatgacg tcttacggaa ggcgtacgca
     4981 ggtggttcca ggaagggtaa ctgtgccacg caagtttcgc gacaaacgca cggcccagtt
     5041 tgcagcaggt atgcgtgtga gggccttctc tgggttcgag cgtcaagcgg acaagcggct
     5101 gacagcactc atggcggcgg aaacgctcaa cgatctcagg gttaaccgag gaaaccggca
     5161 ggagcaactc tcgggggaca ggaacggtca gtggtcgatt cgcatcaacg atcaatggcg
     5221 catctgcttt gaagggactg aggatacaat cgatcccacg gaaatcgaaa tcgtcgatta
     5281 tcactgagga aagtcatgac ggcgcatcga ccaatacatg caattcatcc cggagaatac
     5341 ctcctggagg agatagaagc acgggacatg actgcggcca atttatctcg tgctctcggc
     5401 gttccccaga accggatctc cgagatactc cgtggacgtc gcgctatcac cgtagatacc
     5461 gctcttcggc ttgcccgctg gatcgggtca tctccacaat actggctgaa tctgcagcaa
     5521 acatacgatc taagagttgc tgaacaagcg ctttggcctg agatcgagcg tacggttacg
     5581 cccgattcct ctccaattca tgcagcatgg ccagcgttcg taaccggtcc agcaaccaat
     5641 tccaataggg agggggcggg cgctgaagtt gg
//
LOCUS       DASYWM010000096        11299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_659839, whole genome shotgun sequence.
ACCESSION   DASYWM010000096 DASYWM010000000
VERSION     DASYWM010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11299
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659839"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VGR29_03815"
     CDS             <1..645
                     /locus_tag="VGR29_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV2072748.1"
                     /translation="MNLGQILETHLGWAASTLGFKVATPVFDGADEDDIRDALREAGL
                     PEDGKVDLYDGRTGEKFDRPVTVGIVYMLKLAHLVEDKIHARSTGPYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAHILQEMLTVKSDDIVGRVKTYESIVKGEEIGEAGVP
                     ESFKVLVKELRSLGLSIDVINEDEQTVDFAEDNSRDLLSNLDRINLSGFERTSD"
     gene            837..5300
                     /gene="rpoC"
                     /locus_tag="VGR29_03820"
     CDS             837..5300
                     /gene="rpoC"
                     /locus_tag="VGR29_03820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV2072749.1"
                     /translation="MLDVNNFDAIRIRLASPEAIRSWSFGEVTKPETINYRTLKPEHG
                     GLFCERIFGPTRDWECYCGKYKRIRHAGTVCDKCGVEVTRSKVRRERMGHIELAAPVA
                     HIWYVKGTPSRLGLLLDISPRNLERVLYFASYLVTDVNTEKRDAAIEDIKALSQETID
                     ELRAEAQEQIDEIIASSDIDVSSLEEGSLNLVESIQAEVRRQLDALETSYNEVRENLE
                     ARQGQSSTGQMKWESRIIVKKGEAINEDRLEKLRTRYEERVNEINAEAASARTGSSIM
                     AGAEGDLISHEAAQAIKKIESERDEKIGLEEEQRDEIVKQLQRLAERLILTETEYREL
                     TEIVSPDVFSAAMGAEAVYDLITKKVNLDELAVSLRVDMQATSDVKRKKATKRLRVVE
                     ALRKSGNKPQWMIFTALPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLL
                     ELGAPDIIVRNEKRMLQEAVDALIDNGRRGRVVSGSGKHKLKSLSDMLKGKQGRFRQN
                     LLGKRVDYSGRSVIVVGPELALDECGLPKRMALELFKPFVMRRLVDRELAHNIKSAKR
                     LVERVNPAVWEVLEDVIKDYLVLLNRAPTLHRLGIQAFKPRLIEGSAIQLHPLVTTAF
                     NADFDGDQMAVHVPLSKAAQAEAHERMLSVRNLLAPSSGEPIVSPTQDIVLGVYYITS
                     ERDYEEELAKGTVPKGWGKVFSSLDEVELAYNSGAVDVQAMIYVRTDRDGGEMKRVKT
                     TVGRALFNNILPTSLGKYYNETMGRKQLRRVVADCFRTYKDPFETAKVVNDIKTLGFK
                     MSTKGGLSISLSDVTESETKAALVEAAEDEAARIERQYRRGLVTEDERLRELERIWNA
                     ARDQLTDDVEERLKGQNAVYMMADSGAKGNMNQISQMAGMRGLVLDPGGKVIDIPIKS
                     NFREGLTVLEYFLSTHGARKGLADTAIRTADSGYLTRRLVDVAQDVIITIEDCGTEHG
                     VWATNIDSDGRRMGDEEFRSRITGRILAEDIVHPQTGEIIATRGEELSEFYPNGDGTF
                     RDLVKEVMDAHSNDGTDEDATFRFRIRTVMTCEAEHGACQMCYGRSLATGDLVKLGEA
                     VGIIAAQSIGEPGTQLTMRTFHSGGVNRADITTGLPRVEELFEGRQPKGAALMANKPG
                     VVKIEEDDNGRRLIISAVEEELWRHHLPESWEISYAAGEEVVKGKTVLATGPDGEKLT
                     SDLDGRFTVDGRMIYVHAQREEREEFGVNVGDQLLVEDGQQVTPGIQLTYGAKDPHQI
                     LHTLGAEEVQHYIIDEVQKVYRSQGVNTNDKHIEVICRQMLRKVQIQYPGDTDLLEGD
                     TVDRFEFNTKNDQVLSQGGEPATAIPLLLGITKASLETDSFLSAASFQETTRVLTEAA
                     INGKVDHLRGLKENVIIGKLIPAGSGFDARHAPQEPDALEEATLAAMRLSAEPAEEED
                     YAELMAGGHIPAVQVLEHPGDQDGFNLQAPEDAGEINTADLANETVTGEES"
     gene            complement(5384..6250)
                     /locus_tag="VGR29_03825"
     CDS             complement(5384..6250)
                     /locus_tag="VGR29_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV2072750.1"
                     /translation="MKYAVFTVSTPSMTPEEVAPKLKEYGYDGIEWRVVDEKPNPPGM
                     GFWHGNKSTIAFTGLEDSVPRIRHLAEENGLEMPALGTYVRASAPADVEAAMRGAVAL
                     GVKRLRVNVARYDSSEPFMPVWNRDREDYTRVAELAQKHGVQAQIEIHHGSVCPSASA
                     ARLYVEGMDPEHVGVIHDAGNMVYEGFENYRMGLEMLGPYLAHVHVKNARWFPRKYRD
                     DRSVIWTCDAAPLHRGIAEIRDLFRALKAVGYDGWISVEDFSTERPLDDRLKENLSFL
                     KAVEAEANSPAG"
     gene            complement(6271..6768)
                     /locus_tag="VGR29_03830"
     CDS             complement(6271..6768)
                     /locus_tag="VGR29_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2072751.1"
                     /translation="MNQAIHTVDLLQWLMGGVASIQAHRSTLTHDIVTEDVASASFRF
                     NNGALGSITATTSASSDYPVRVEVIGTEGRVTLENNVVTLWEAARPLGDSLLTDEDRE
                     LVEGWRAGEPFGAGHTRQLKTIFKCLAGGVQAPVAGAEARRAVDIILGIYESSRTGAR
                     VKFER"
     gene            complement(6695..7291)
                     /locus_tag="VGR29_03835"
     CDS             complement(6695..7291)
                     /locus_tag="VGR29_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2072752.1"
                     /translation="MNSPFKVGISGGGTIGRVHAQALAQISSADLVAVAEPQEDPGRR
                     LASEQEASWYRSIDEMLEQGEIDVVIVATPSGLHPDQAGKHVITEKPMAITADGIDRM
                     ITACAEADIQLAVIFQNRLSTHVMQVRRAIERGHLGRPVLANGVMYWYRSQEYYQANR
                     GLARHLESGWWRRADEPGNPHRRSAPVAYGWCCLHSGT"
     gene            7656..8078
                     /gene="rpsL"
                     /locus_tag="VGR29_03840"
     CDS             7656..8078
                     /gene="rpsL"
                     /locus_tag="VGR29_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV2072753.1"
                     /translation="MPTINQLVRKGRKSVKKKTKSPALRFTNSSLGRYAGTLRSGTYA
                     PQKRGVCTQVKTMTPKKPNSALRKIARVRLTSGVEVTAYIPGEGHNLQEHSVVLIRGG
                     RVKDLPGVRYHIVRGALDARGVENRKQARSKYGTKAPK"
     gene            8415..8885
                     /gene="rpsG"
                     /locus_tag="VGR29_03845"
     CDS             8415..8885
                     /gene="rpsG"
                     /locus_tag="VGR29_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV2072754.1"
                     /translation="MPRRAKIERRVPTPDARYNSELVARFINKIMQRGKKGLAERIMY
                     DALQTIQERTGRDPVEVFEQAVSNATPVLEVKPRRVGGATYQVPVQIEGARRQSLAIR
                     WLLGSARARAGKTMSEKLANELLDAFNGTGATIKRREDTHRMAEANRAFSHFRV"
     gene            8972..11086
                     /gene="fusA"
                     /locus_tag="VGR29_03850"
     CDS             8972..11086
                     /gene="fusA"
                     /locus_tag="VGR29_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV2072755.1"
                     /translation="MSTATSTKSATTGRAGEGLRSTRNIGIIAHIDAGKTTTTERILF
                     YTGRVHKAGETHEGAATMDWMAQERERGITITSAATTCFWREHRINIIDTPGHIDFTV
                     EVERSLRVLDGGVVVFDGVAGVEPQSETVWRQADKYHVPRICFVNKMDRTGANFERTV
                     QMIVDRLKAKPAPVQLPIGAESSFQGVIDLIHMKAITYHDDEGKDIRIVEIPEDHREA
                     AEAARLTLLEAVAETDEDLMMRYLEGEELSADELVAAIRAATLTSEIVPVLCGTALKN
                     KGVQPMLDAIVDYLPSPLDVPPVKAIDAKTEEEVIREPDPDGPLAALAFKIAVDPHVG
                     KLCYVRVYSGTLKSGSYTLNTSKGDRERVGRLLLMHANHREEVDEITAGNICAVIGLK
                     STTTGDTLSDLNEPVILESITFPEPVISVSIEPKTRSDQDKMGAALARLAEEDPTFRV
                     RTDEETGQTVLDGMGELHLEVLVDRMMREFRVEANVGRPQVAYRETITVPVKAEGRHV
                     RQSGGKGQYGHVVVEFEPLERGAGYEFVDQIVGGSIPREFINPTNAGIREALDTGGAA
                     GFPLVDVRARLVDGSYHDVDSSEMAFKIAGSLSVKEAVRRGRPNILEPIMKVEVITPE
                     EFMGDVIGDLNARRGLIQAMSDRAGAQVINAFVPLSNMFGYTTDLRSMTQGRAMSSME
                     FDHYAPLPENLAAEFVAKANRT"
     gene            11172..>11299
                     /locus_tag="VGR29_03855"
     CDS             11172..>11299
                     /locus_tag="VGR29_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV2072756.1"
                     /translation="MGKAKFERTKPHVNVGTIGHVDHGKTSLTAAISKTLAMQGGA"
BASE COUNT         2329 a         3258 c         3546 g         2166 t
ORIGIN      
        1 atgaatctcg ggcagattct cgagacgcac ctaggctggg cggcaagcac gctcggattc
       61 aaggtcgcga cacctgtctt tgacggtgcc gacgaagacg atattcggga cgctctgcgg
      121 gaggctggct tgccggagga cggcaaggtg gatctgtacg acggccggac gggtgagaag
      181 ttcgatcgcc ccgtgaccgt cggtatcgtc tacatgctga agcttgctca cctggttgag
      241 gacaagattc acgcccggtc gactggtccg tactcgcttg ttacgcagca gccactgggt
      301 ggtaaggccc agttcggtgg acagcgcttc ggcgagatgg aagtctgggc gctggaagcc
      361 tacggtgccg cgcacatcct gcaggaaatg ctcacggtca agtccgacga catcgttgga
      421 cgcgtgaaga cctacgagtc gatcgtcaag ggtgaagaga ttggcgaggc cggcgtgccg
      481 gaatcgttca aggtgcttgt caaggagctt cgcagccttg ggctctcgat cgatgtcatc
      541 aatgaagacg agcagacggt ggacttcgcg gaggataact ctcgcgatct gctgtcgaat
      601 ctcgatcgta tcaatctgag cggcttcgaa cggacgtctg actgatcttc cgcgtacatg
      661 caccacggtg actggcgtac cacgccggtc acctgcccct ttagtcagcg tcggtttcga
      721 gtggaggaac catgtcaagc aactataccc ctgaagtcgc tcctacgatg gtggaccagc
      781 gtccacagag tggcgctggt ggatactcgc gcggcgatcg taacgatcgt ggtcgggtgc
      841 tcgatgtcaa taatttcgat gcaattcgca ttcggctcgc ttccccggag gcgatccggt
      901 cctggtcgtt cggtgaggtt accaagcccg agacgatcaa ctaccgaaca ttgaagccgg
      961 agcacggcgg tctcttctgc gagcggattt ttggtcccac gcgagactgg gagtgctatt
     1021 gcggcaagta caagcgaatt cgccacgccg gaaccgtctg cgacaaatgc ggcgtcgaag
     1081 tgacccgctc gaaggtccgc cgtgagcgga tgggccatat cgagctcgcc gctccggtcg
     1141 cccatatctg gtacgtcaag ggcacgccga gccgtctcgg tctgttgctc gacatctccc
     1201 cacgcaacct cgagcgcgta ctctacttcg cctcctacct ggtgacggat gtcaacaccg
     1261 agaagcggga tgccgccatc gaggacatca aggcactttc gcaggaaacg atcgacgagc
     1321 tgagggctga ggcgcaggag cagattgatg agatcatcgc aagcagcgat atcgatgttt
     1381 ccagccttga agagggttca ctgaacctcg tcgagagcat tcaggcggaa gtccggaggc
     1441 agctcgatgc ccttgagacc tcctacaacg aggtaaggga gaacctcgag gctcgccagg
     1501 gccagtcttc caccggccag atgaagtggg aaagccggat catcgtcaag aagggcgagg
     1561 cgatcaatga ggatcgtctt gagaagctca ggactcgcta cgaagagcgg gtgaacgaga
     1621 tcaacgccga ggctgcttcc gcccgcaccg gtagctcgat catggccggc gccgagggtg
     1681 acctgatcag ccatgaggcg gcacaggcta tcaagaagat cgagtccgag cgcgacgaga
     1741 agatcgggct ggaagaagag cagcgcgacg agattgtcaa gcagttgcag cgactcgccg
     1801 agcggctcat cctcacggag acggagtacc gagaactgac cgaaatcgtc tccccggacg
     1861 tttttagcgc cgcgatgggt gccgaggctg tctacgacct gatcaccaag aaggtgaacc
     1921 tggacgagtt ggccgtatcg cttcgcgtag acatgcaggc aacgtccgat gtcaaacgga
     1981 agaaggcgac caagcggctg cgcgtcgttg aggccctccg caagagtggc aacaagccgc
     2041 agtggatgat cttcactgcg ctgccggtca ttccgccgga gcttcgtccg atggtccagc
     2101 tggacggtgg ccgctttgcg acctcggact tgaacgacct ctaccgtcgc gtcattaacc
     2161 ggaacaaccg cctgaagcga ctgcttgaac tcggtgctcc ggacatcatt gtccgcaacg
     2221 agaagcggat gcttcaggaa gccgttgacg cactgatcga caatggccgc cgcggccggg
     2281 tggtgtcggg ctcgggcaag cacaagctga agagcctgag cgacatgctg aagggcaagc
     2341 aggggcgatt ccgccagaac ctgctcggca agcgtgtcga ctactccggt cggtccgtga
     2401 tcgtcgtcgg tccggagctg gcgctggatg agtgcggcct gccgaagcgg atggcgctcg
     2461 aactcttcaa gccattcgtg atgcggcggc tggtcgatcg tgagctcgcg cacaatatca
     2521 aatcagccaa gcgacttgtt gagcgcgtca accctgccgt gtgggaagta ctggaagacg
     2581 tcatcaagga ttacctggtg ctgctcaacc gtgcaccaac cctgcaccgg ctcggcatcc
     2641 aggcattcaa gccacgcctc attgaggggt cggcgattca gctccatccg ctggtgacga
     2701 cggccttcaa tgccgacttc gacggtgacc agatggcggt tcacgtgccg ctctccaagg
     2761 cagcgcaggc tgaggcccac gagcggatgc tatccgtccg gaacctgctt gcgccgtcca
     2821 gtggtgagcc gatcgtatcg ccgacgcagg atatcgttct cggcgtttac tacattacga
     2881 gcgagcgcga ttacgaggaa gagcttgcca aggggaccgt gccgaagggc tggggcaagg
     2941 tgttctcgtc cctggacgag gttgagctgg cctataactc cggcgcagtc gacgtgcagg
     3001 cgatgatcta cgtccgcact gatcgggatg gcggcgagat gaagcgcgtc aagacgaccg
     3061 ttggtcgtgc gctcttcaac aacatcctcc cgacatcgct gggcaagtac tacaacgaga
     3121 cgatgggtcg caagcagctc cgtcgcgtgg tggctgactg cttccgcacc tacaaggatc
     3181 cgttcgagac ggccaaggta gtcaacgaca tcaagaccct cggcttcaag atgtcgacaa
     3241 agggcggtct atcgatctcc ctgtctgatg tcacggaatc tgagacgaaa gcggccctcg
     3301 tggaggctgc cgaggatgag gccgcccgga tcgagcgtca gtatcgtcgt ggtctcgtga
     3361 ctgaggatga gcggctccgc gagctggagc gcatctggaa cgcggcccgt gaccagttga
     3421 ccgacgacgt tgaggagcgg ctcaagggcc agaacgccgt ctacatgatg gcggactcgg
     3481 gcgcgaaggg aaacatgaac cagatcagcc agatggccgg catgcgcggc ctcgtgctgg
     3541 atccgggcgg gaaggtcatt gacatcccga tcaagtcgaa cttccgtgag ggcctgaccg
     3601 tactggagta ctttctctcg acgcacggtg cccgtaaggg tctcgctgat accgcgatcc
     3661 gcacggccga ctcaggttac ctgacccggc ggcttgtcga cgtagcccag gatgtcatca
     3721 tcacgattga ggattgtggc accgagcatg gggtttgggc gacaaatatc gactcggacg
     3781 ggcgacggat gggcgatgag gagttccgct cccgcatcac cggacgtatt ctggccgagg
     3841 atatcgtcca tccgcagacg ggggagatca tcgccacaag gggagaggag ctctcagagt
     3901 tctacccgaa tggagacggc accttccggg atcttgtcaa agaagtgatg gacgcgcact
     3961 cgaacgatgg caccgacgaa gatgcaacgt tccggttccg cattcgcacg gtcatgacat
     4021 gcgaagccga gcatggcgct tgccagatgt gctacggccg cagccttgca acgggcgatc
     4081 tcgtcaagct tggcgaagcg gtcggcatca tcgcggcgca gtcaatcgga gagccaggca
     4141 cgcaactcac gatgcgcaca ttccactctg gtggagttaa ccgcgcagac atcacgacgg
     4201 gtcttccccg cgtcgaggag ttgttcgagg gacgccagcc gaagggcgcg gcgctcatgg
     4261 cgaacaagcc gggtgtcgtg aagatcgaag aggatgacaa tggccgacgg ctgatcatca
     4321 gcgccgtcga ggaagagctc tggcgtcatc acctgccgga gtcctgggag attagctacg
     4381 ccgcaggtga ggaagtggtc aagggcaaga cggtcctggc aaccggtccg gacggcgaga
     4441 agctcacgtc ggacctggat ggacggttca ctgttgatgg ccggatgatt tacgttcacg
     4501 cgcagcgcga ggagcgtgag gagtttggtg tcaacgtagg cgaccaattg ctcgtcgagg
     4561 acgggcagca ggttacgcca ggcatccagc tcacctacgg tgcgaaggat cctcatcaga
     4621 tcctgcacac cctgggcgcc gaagaggtgc agcactacat catcgatgaa gtgcagaagg
     4681 tgtaccgctc acaaggcgtg aataccaatg acaagcacat tgaggtgatt tgccgtcaga
     4741 tgctccggaa ggtccagatc cagtaccctg gtgacacgga tcttctcgaa ggggacacgg
     4801 tcgatcgctt cgaattcaac acgaagaacg atcaggtgct ttcccagggc ggggagccgg
     4861 cgacagcgat tccgctgttg cttggaatca ccaaggcatc gctcgagacg gattccttcc
     4921 tctctgccgc gtcgttccag gagaccacgc gtgtgctgac tgaggccgcc atcaacggta
     4981 aggtcgatca cctgcgcggt ctcaaggaga acgtcattat cgggaagctc attccggctg
     5041 gttccggctt cgatgcccgg cacgcgccgc aggagcccga tgcgctggag gaggcgacac
     5101 tcgcggcgat gcgcctgagc gccgagcccg ctgaggaaga ggattacgcc gagttgatgg
     5161 ctggcggtca catccccgcc gtccaggtgc tcgagcatcc aggtgaccag gatgggttca
     5221 accttcaggc gcctgaagat gccggtgaga tcaatacggc cgatctcgcg aacgagacag
     5281 tcacgggcga agagtcctaa cccgaactca caacccatcg aagcagaaca ccccggtacc
     5341 caacggtacc ggggtgttct gcttattgga cgatcagagg aaactatcca gcagggctat
     5401 ttgcttcggc ctcaactgct ttcaggaagg agagattctc cttcagtcgg tcgtccagtg
     5461 ggcgctcggt agaaaaatcc tcgacgctta tccacccgtc gtagcccacc gccttcagtg
     5521 cccggaagag atcgcggatc tcggcaatgc ccctgtgcag gggggctgcg tcgcaagtcc
     5581 agatgacgct cctgtcgtcc cgatacttcc gggggaacca gcgcgcgttt ttcacatgga
     5641 cgtgcgcgag atagggcccg agcatctcaa gacccatgcg gtagttctca aacccctcat
     5701 acaccatgtt gccggcgtcg tgaatgactc cgacgtgttc cgggtccatc ccttccacat
     5761 acaaacgagc cgcgctggcc gagggacaaa cagaaccatg atgaatctcg atttgcgcct
     5821 ggacaccatg cttttgggcc agctccgcga cgcgcgtgta gtcttcccga tccctgttcc
     5881 ataccggcat gaagggttct gaactgtcgt agcgggccac gttcacgcgg agacgcttca
     5941 cgccgagggc aaccgcaccc ctcatcgccg cttccacatc tgctggcgct gatgctcgga
     6001 catatgtgcc gagagcaggc atctccaacc cgttctcttc ggcaagatgc cggattcttg
     6061 gcacactgtc ctcaagaccc gtgaaggcga tcgttgattt gtttccgtgc caaaagccca
     6121 ttcctggcgg gttcggcttc tcgtcgacca cgcgccattc gattccgtcg tacccgtact
     6181 ccttcaactt tggagcaact tcttccgggg tcatggacgg ggtggagacg gtgaagacgg
     6241 cgtatttcat tgtatctatt cccttgcaat ctagcgttca aatttcaccc gggcgcctgt
     6301 gcgggatgat tcgtagatcc caagaatgat gtctaccgcc ctgcgtgcct cggcaccagc
     6361 gactggtgct tgaacaccgc cagcgaggca cttgaaaatc gtcttcaatt ggcgggtatg
     6421 tcctgccccg aagggttcac cggctcgcca tccctcaacc agctcgcgat cttcgtctgt
     6481 cagcaggctg tcgcccagcg gtctggctgc ttcccacagc gtcaccacgt tgttctccag
     6541 cgtgacacga ccctccgtgc caatcacttc cactcgcacg ggataatcgc tggaagccga
     6601 tgtagtcgcc gtgatgctgc cgagcgcgcc attattgaac cggaaactgg cagaggcgac
     6661 atcctcggtc acaatatcgt gggtcaacgt tgacctatgt gcctgaatgg aggcaacacc
     6721 acccataagc cactggagca gatctacggt gtggattgcc tggttcatca gcgcgccgcc
     6781 accatccaga ttccaggtgc cgcgccagcc cccggtttgc ctggtaatac tcctggcttc
     6841 gataccagta cattacgccg ttggcgagca caggtctccc aaggtgcccg cgttcgattg
     6901 ctcgtcgaac ctgcatcaca tgggtggaca ggcggttttg gaagataacc gccaattgga
     6961 tatccgcctc cgcgcacgcg gtgatcatgc ggtcgattcc gtcggcggtg atcgccattg
     7021 gtttctcggt gatcacatgc ttgcccgcct ggtccggatg cagcccggac ggagttgcga
     7081 cgatgacaac gtcgatctca ccctgttcga gcatctcgtc gatcgagcgg taccagcttg
     7141 cctcctgctc ggaggccagg cgtctgccgg ggtcctcttg cggctccgca actgcaacga
     7201 gatcagcgct gctaatctgt gccaatgcct gtgcgtggac ccgaccgatg gtgccgccgc
     7261 cggaaatgcc aaccttgaat ggtgagttca tgcgtggaac cttcctgccg gtggagaacc
     7321 ggcgatatgc gcttgccaag gttccagaac atgctatcca gtaggtgcga ggatcgcaag
     7381 acgtgttacc tctcacgagc ccggacaagg gcgtggggtt gaatggcccg tgattatcgc
     7441 ctatacttgc ctgtcgtggc acccatgggt agtcgcggag tgcggttgtg gcctctgatc
     7501 gcattattcc aaattcccga ggtctactgt atcaaccgag acgtgccagg cggcaccgca
     7561 gctgtggcgc tcttcccgag cgcgctccgg aacgtggggg aaccctgttc cggcacagag
     7621 cacgggtgag ccccctgcac ggataggagc ttttcgtgcc gacgatcaat cagttggttc
     7681 gcaagggccg caagagcgtc aagaagaaga ccaagtcacc agcgcttcgt ttcacaaaca
     7741 gctcgctggg tcggtatgct ggcacactgc gaagcggcac gtatgcgccg cagaagcggg
     7801 gtgtttgcac ccaggtcaaa accatgacgc cgaagaagcc caactcggca ctgcgcaaga
     7861 tcgcccgtgt gcgccttaca agtggcgttg aagtcacagc gtacattcct ggcgaagggc
     7921 acaatctgca ggagcactcc gtggtgttga tccgcggcgg ccgggtgaag gatctcccgg
     7981 gcgttaggta tcacatcgtt cgtggtgcgc tcgatgcccg cggtgttgaa aaccgcaagc
     8041 aggcgcggtc caagtacgga accaaggctc ccaaataggg tgcgggatat ccgcatctga
     8101 tgggagttac cgctacggcg gaactgagag agctgagata cgggaagcgg agcggcgtct
     8161 aaggtggacg ctacccgctt tttcgtgtgt cagccgctct tgggccttgc gaacctgaac
     8221 aagagtcaat aatgaggtga gtaggactcc ggtggatggt tgtcgtgtgc cggggcagtt
     8281 gaacgcagag agcggcagcc aggtggttgt tccgcttgat ggctggtaag agacgtggtc
     8341 gcggcaggga ttcgaagggg agaaggctcc acccggatct aaatcagcgt cacacacctg
     8401 aggcaggaga accaatgccg agacgagcga aaatcgaacg tcgagtgccg acgccggacg
     8461 cacgctacaa cagcgagctg gtggcacggt ttatcaacaa gatcatgcag cggggcaaga
     8521 agggccttgc tgagcggatc atgtacgacg ccctgcagac tattcaggag cgcactggtc
     8581 gtgatccggt tgaagtcttt gagcaggcgg tttcaaatgc aacccctgtc cttgaggtga
     8641 agccacgccg tgtcggcggt gcaacctatc aggtgccggt gcagatcgag ggcgcgcggc
     8701 ggcagtcgct ggccattcgc tggctgctgg gatctgctcg cgctcgagcc gggaagacga
     8761 tgtccgagaa gctcgctaac gagctgctcg atgcgttcaa tggcacaggt gccaccatca
     8821 agcggcgcga ggatacccac cgcatggcgg aggcgaaccg agccttctcg cacttccgcg
     8881 tctagttcgg acgcggtcac ggcagcgtcg gacgacgttg tccacgccga ggccggacac
     8941 gttttgtgta tcgcgaggac gaacccattc aatgagtaca gcaacttcaa caaagagtgc
     9001 cacgactggc cgcgccgggg aaggactccg ttcgacgcgt aacatcggaa tcattgctca
     9061 catcgatgcc ggcaagacga cgacgaccga gcggattctg ttctataccg gacgcgtcca
     9121 caaagccggt gaaacgcacg agggcgcggc caccatggac tggatggcgc aggagcgtga
     9181 gcgtggtatc acgatcacct ctgctgccac cacctgtttc tggcgcgagc accgcatcaa
     9241 tatcatcgat acaccgggtc acatcgactt cacggtcgag gtagagcgtt cgctccgagt
     9301 cctcgacggt ggtgttgtcg tcttcgacgg ggtagccggt gtggagccac aatcggagac
     9361 ggtgtggcgc caggctgaca agtatcatgt tccccgcatc tgcttcgtca acaagatgga
     9421 ccgcaccggt gcgaacttcg agcgcacggt tcagatgatt gtggatcgtc tgaaggcgaa
     9481 gccggctccg gttcagttgc ctatcggcgc ggagtccagc ttccagggtg tgatcgacct
     9541 cattcatatg aaggcgatca cttaccacga tgatgaaggc aaggacatcc ggatcgtcga
     9601 gattccggag gaccaccggg aggccgctga ggccgctcgc ctgacgttgc ttgaggccgt
     9661 tgccgagacc gacgaagatc tcatgatgcg ctacctcgaa ggcgaggagc tgagtgccga
     9721 cgagctggtg gccgccatcc gtgccgccac gctgaccagt gagatcgtcc cggttctctg
     9781 tggcaccgcc ctgaagaaca agggcgttca gccaatgctg gacgccatcg ttgactacct
     9841 tccatcaccg cttgacgtcc cgccggtcaa ggcgatcgat gccaagacgg aagaggaagt
     9901 cattcgtgag ccagatccgg acggaccgct ggcggcgctt gcattcaaga tcgccgtgga
     9961 tcctcacgtc ggcaagctct gctacgtccg cgtgtactcc ggtaccctga agtccggctc
    10021 ctacacgctc aacacctcga agggtgatcg cgagcgcgtt ggtcgcctgt tgctcatgca
    10081 tgcgaatcac cgggaagagg tcgacgagat cactgccggt aatatctgtg ccgtcattgg
    10141 actcaagtcc acgaccacgg gtgacacgct gagcgacctg aacgagccag tgatcctgga
    10201 atccatcacc ttcccggagc cggtgatctc ggtctcgatc gagcccaaga cccgctctga
    10261 ccaggacaag atgggtgccg cccttgcgcg tctcgcagaa gaggatccga ccttccgggt
    10321 ccgcaccgac gaggagactg gacagacggt cctcgatggg atgggagagc ttcacctcga
    10381 agtactcgtg gaccgcatga tgcgtgagtt ccgggtagag gcaaacgtcg gtcggccgca
    10441 ggtcgcctat cgtgagacca tcacggtacc ggtcaaggcc gaaggacgcc acgtccggca
    10501 gtcgggtggt aagggccagt atggccacgt cgttgtcgag ttcgagccgc ttgagcgcgg
    10561 tgcgggctac gagtttgtcg accagatcgt tggtggctcg attccccggg agttcatcaa
    10621 cccgaccaac gctggcatca gggaagcgct ggacactggc ggagccgccg gattcccgct
    10681 ggttgatgtg cgggcgcggc ttgttgacgg ttcctaccac gatgttgact cgtcagaaat
    10741 ggcgttcaag atcgccggtt ctctctccgt caaggaagca gtccgacgcg gtcggccgaa
    10801 catccttgag ccgatcatga aggtcgaagt catcacgccg gaagagttca tgggcgatgt
    10861 catcggagac ctgaacgcgc ggcgtggcct gattcaggca atgtcagatc gtgctggtgc
    10921 tcaggtgatc aacgccttcg tgcctctgtc gaacatgttc ggttacacga ccgatcttcg
    10981 ttcgatgact cagggacgag cgatgtcgtc gatggagttc gaccactatg cgccgctgcc
    11041 ggaaaacctg gccgcggagt tcgtggcgaa ggcaaaccgg acctaacgta gtaaaatctt
    11101 tggttgggtc gccgttatgg tggcggctta cctgatcggg cggccagagg tcgctcaggg
    11161 agtggatgat tgtgggtaag gcgaagtttg agcggacgaa gccgcatgtg aatgtgggga
    11221 cgatcgggca tgtcgatcat ggcaagacga gcctgacggc ggcgatctcc aagaccttgg
    11281 cgatgcaggg gggggcgaa
//
LOCUS       DASYWM010000097         6947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_3510, whole genome shotgun sequence.
ACCESSION   DASYWM010000097 DASYWM010000000
VERSION     DASYWM010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3510"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..547)
                     /locus_tag="VGR29_03860"
     CDS             complement(164..547)
                     /locus_tag="VGR29_03860"
                     /inference="COORDINATES: protein motif:HMM:NF025842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072757.1"
                     /translation="MTQSLEFTALGKNQTHFTGLDVFDRPEHVVEVVLSGDELTAFCP
                     ITHQPDFYSYEVRYRPVTSCIESKTFKLLIGSFREQAAFAETLASDLAQQVHDATGAD
                     TEVILTQQVRGGVSLTAKASIPRAS"
     gene            complement(1163..1405)
                     /locus_tag="VGR29_03865"
     CDS             complement(1163..1405)
                     /locus_tag="VGR29_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072758.1"
                     /translation="MQPVTQISNSAEALNRSIDRFLLAAGWRVTDVVKTADGTGYDVT
                     LTKSTEPNLGRSVTRSASSAGEATLKACSSARLPIR"
     gene            complement(1759..2832)
                     /locus_tag="VGR29_03870"
     CDS             complement(1759..2832)
                     /locus_tag="VGR29_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2072759.1"
                     /translation="MPESDVGIRNLVKRFGGTVAVRGMSLEVPRGAFVTFLGPSGCGK
                     TTTLRMIGGFEQPDSGQILIKGEDAGNQPPHKRDTSMVFQSYALFPHMSVAHNIGFGL
                     RERKVPKHEIEQRVAEMLKLIELPEVGDRKPHELSGGQQQRVALARSLVVKPSVLLLD
                     EPLGALDLQLRKQMQIELKRIQRRLSITFIYVTHDQEEALSMSDRIVVMNDGRVEQEG
                     DPEEIFSRPRTRFVAEFMGARNILDVDVVDRDATSTRVRLGSGEMTLPVSVPNRGTKA
                     TLVIRPEKVRINAESGWDGRVIERVYKGPLMTYLVGLDNGMEIYADTAHDDTCQRYEI
                     GDETRLNFRTEDVVLIPEHAKAT"
     gene            complement(2973..3755)
                     /locus_tag="VGR29_03875"
     CDS             complement(2973..3755)
                     /locus_tag="VGR29_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072760.1"
                     /translation="MPDRMHDAAALLLRHWNKGTRLDALPEELRPATREEGYALQAAL
                     AEVSGDALYGWKIAATSEAGQRHINVDGPLAGRLLSRCVIADGAMVSLVTNLMRVAEL
                     EFAFRLGADLPPRPETYTVDEVMAAVVTLHPAIEIPDSRFHDFTVVGAPSLIADNACA
                     NLFLPSPGLATAWREVDLAAYDVTAVVNRKERHVGVGLNVLGDPRVALAWLVNEVTSL
                     GLPVQVDQMVTTGTCVTPIAVAPGDEVRGDWGAFGTLSVRFA"
     gene            complement(3817..4482)
                     /locus_tag="VGR29_03880"
     CDS             complement(3817..4482)
                     /locus_tag="VGR29_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072761.1"
                     /translation="MVRIERDAPERGALPPELERWRLVPVSIVVDLNLEPGQIDPAIR
                     PLMPPGQQPRLFGRSVTALCDPPDFGAVLHALEVIQPGDVLVIAAGGDAHTAMIGEIL
                     CGHVRRLGGAGVVCDGAVRDVATLARWTDLPVFARHVTPRGPSGTGRGAVNVPVEVGG
                     ATVSSGDLIIGDDDGLVALSPASIRDGIAGAEAKLALETEWEASLARGVAVGRVFGFS
                     EAE"
     gene            complement(4853..5590)
                     /locus_tag="VGR29_03885"
     CDS             complement(4853..5590)
                     /locus_tag="VGR29_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV2072762.1"
                     /translation="MDLARMEFFSRALGKRASYNVLLPETGEGPFPVLFQLHGLTDDC
                     NTWIQRSNVALHANAYPMVIVFPDGGTSAYLNWKAAGRLGRQNYEDLIITDISDHVRR
                     HLHVTDGPWAIGGLSMGGWGAMWLGLKYPDRFASIWSHSSKFNWRDSDLDFSMLAQPE
                     DVDLIAYAERISTLEQKPVISFDCGVDDQLIEENRDFHNHLEKIGLEHHYTEHSGGHT
                     WVYWDDHVREALAQHARVVGLTMSGQA"
     gene            complement(6015..6758)
                     /locus_tag="VGR29_03890"
     CDS             complement(6015..6758)
                     /locus_tag="VGR29_03890"
                     /inference="COORDINATES: protein motif:HMM:NF021617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEV2072763.1"
                     /translation="MDDELMAAREQAQKHAEDVRGFVQHAGAFIAVGLFFLVMNLLTG
                     PDSLWFYWPMLPWSIGFLIHGWNVFWDKRFFDEEWQERQVNKTLARMRTGEPPSSGTM
                     AATEIQEILDRASGLVDGMRSSARRIPDPDVRHKALDACAASDQVLAAIIDHPDELPL
                     ARDFVNRFLEPANAVMSDYSRLATRNVPSARETLSKVEQHDLPLITHKANQLYDRLHR
                     GTLIDLEVAREMLTLDMANTSVPELPARD"
BASE COUNT         1385 a         2281 c         2003 g         1278 t
ORIGIN      
        1 tcacccgagg gggtgagatc accgggccag accggaatca gccacggccc gcccggaaaa
       61 cggatcgttc cccaatgggt caggagccag ttcgcaaacc agatcgaggc aatgaacccg
      121 atgaaataga gcacgccttc gtagttgcga atgtggttcg tcttcatgag gcccgcggaa
      181 tcgatgcttt cgccgtcagc gatacgcccc cgcggacctg ctgcgtgagg atgacctccg
      241 tatcggcccc ggtcgcgtca tggacctgct gggcaagatc actggccagc gtctcggcaa
      301 atgcggcttg ctcccggaag gacccgatca ggagcttgaa cgtcttggac tcgatacagg
      361 atgttaccgg ccggtaccgc acctcataac tatagaagtc cggctggtgc gtgataggac
      421 aaaaggccgt gagctcatcc ccggacagga caacttccac gacatgctcc gggcgatcga
      481 agacatcaag accagtgaag tgggtctggt tcttcccgag agcggtgaac tcaagtgatt
      541 gggtcatgtt tcattccttt cggaacggga cggcgatgcc ccgagtggtg ggatggaccc
      601 gggtcaacag aggcagcagg tttgccaacg cgatagtgtg acacggccct atcaattggc
      661 aacagcgaaa cgttctggca tgagagacac ttcactgatc gtgatgatgc tcgcgtaagc
      721 cgaaccatgt cgagatcgat cagcatgagc gctccctttg ctcccgccag tgtgaagata
      781 cgagatcggc tctgtccctg ccacatacct tgggaacggg tcgctcgcac aacgggattt
      841 gcccccgtga ggagtcagca cccggcgaag ttccccgtta gcgcagctcc catatgcgca
      901 ggcaaacact gccacgtccg atccaacggc cgcctgatga tcctggcgga gcgatcagtg
      961 ggcaggagag acgtgcccgt cacgagagcg gcaaagcgaa aaccgaaccg atcctcccca
     1021 caaaaggagg tttggttcgg tatattacgt caggtctggg gtgttgacgc gaggaatgga
     1081 cgagccttgc ccagcggagc agagtcaggg aacacgccac acgagttccg accctgcgcc
     1141 caatgaatgc gatgaggggc agttacctga tcggaagccg cgcggagctg catgccttga
     1201 gcgtagcctc acccgccgat gatgccgagc gggtcacgct ccgccccaag ttgggctccg
     1261 tgctcttggt caatgtaacg tcgtagccgg tgccatcggc cgtcttcacc acgtctgtca
     1321 cacgccagcc agcagcgagg agaaaacgat cgatcgagcg attcagcgct tcggcggaat
     1381 tggagatttg agtgacaggt tgcatgggag agcctcattc ggaacagacg gcgctcccgt
     1441 ccagagatgc aggttctttc ctggatacct tcaattataa gctatgcgat gccgaaaacc
     1501 ctcatcggga tacgggtgcc agcaccttcc tccattgagc gacgaccgaa aggcgttcga
     1561 cgaccggagc atcgagcccc tgcaagcacc gagcctgtcg ccagtgacag caccgctggc
     1621 gcacctaacg cgatctccgg gctgccactc gcccgttctt ttcgatctcc aaagcaccag
     1681 ggccagcagt gaaaccatgg agaggttata cccggccctg gacaaccggt aaaaatcgct
     1741 aacgtcgagg gcaatgggct atgtcgcttt cgcgtgctcg ggaatcagaa cgacgtcctc
     1801 ggtacggaag ttgagcctgg tttcatcgcc gatctcatac cgctggcagg tgtcgtcatg
     1861 agcggtgtcc gcgtagatct ccataccatt atcaaggccc acaagatagg tcatcaacgg
     1921 ccccttgtag actcgctcta tcacccggcc gtcccagcca ctctcggcgt ttatccgcac
     1981 cttttccggc cggatgacca acgttgcctt cgtgccgcgg ttcggcacgg acacaggcag
     2041 cgtcatctct cccgacccaa gcctcacccg cgttgatgta gcatcgcgat ccaccacgtc
     2101 cacatccaga atgttccggg cacccataaa ctcggccaca aaccgcgtcc gtggccggct
     2161 gaaaatttcc tccgggtcgc cttcctgctc gacccgtcca tcattcatca ccacgatccg
     2221 gtcggacata gacaatgcct cttcctggtc atgcgtcacg tagatgaaag tgatgctgag
     2281 ccgccgctgg atccgcttga gttcgatctg catctgcttc cgtagctgga gatccagcgc
     2341 accgagcggc tcgtcaagca ggagcacgct gggcttgacg accagggacc gtgcaagcgc
     2401 gacccgctgt tgctgacccc cggagagctc gtgcggcttc cggtcgccca cctcgggcaa
     2461 ctctattagc tttagcatct ctgcgacgcg ttgttcgatc tcgtgcttcg gaaccttacg
     2521 ctcgcgcagg ccgaacccga tgttgtgagc cacgctcata tggggaaaga gcgcgtagct
     2581 ctggaaaacc atggaggtat cccgcttgtg cggcggctgg tttcctgcgt cctcaccctt
     2641 gatgaggatc tgcccgctgt cgggctgctc gaagccgcca atcatgcgca gggtcgtcgt
     2701 cttgccgcat ccggatggcc cgagaaaagt gacaaatgcc cctcgcggca cttcgagact
     2761 catgccccgc acagccaccg tgccaccgaa tcgcttgacc aggtttcgga taccgacgtc
     2821 tgattcgggc acggatgatc tctccatgag ggcgtggcac cacgccctcg tccatcgcca
     2881 acgagaaccg cttcccattg ataccgtctc gacgcaattt cctcgtggac aggcgattac
     2941 gcaggttcgt cccgacgaag ttgcactcgc gcctacgcga accggaccga gagggtccca
     3001 aacgcgcccc agtcgccgcg gacctcatcc ccgggcgcga cggcgatggg cgtgacgcag
     3061 gtgccggtcg tcaccatctg gtccacttgc accggcaggc cgaggctcgt cacctcattg
     3121 acgagccatg ccagcgccac acgtggatca ccgaggacgt tcaagcccac gccaacgtgc
     3181 ctctctttcc ggttgaccac tgccgtcacg tcgtacgcgg caaggtcgac ctcccgccat
     3241 gccgtcgcca gccccggact gggcaggaag agattcgcgc aggcgttgtc ggcaatcagc
     3301 gagggagcgc caaccaccgt gaagtcgtgg aagcgggaat cggggatctc gatcgccgga
     3361 tggagcgtca ccacagcggc catcacctca tctaccgtgt acgtttcggg gcgcggcggc
     3421 aggtcggcgc cgagccggaa ggcgaactcc agctctgcca cgcgcatcag gttggtcacc
     3481 agcgagacca tcgcgccgtc cgcaattacg cacctggaga gcaggcgccc cgcgagcgga
     3541 ccgtccacat tgatgtgccg ctgcccggcc tcgctggtgg ccgcaatctt ccagccgtag
     3601 agggcatcgc ccgacacctc tgccagcgcc gcctggagcg cgtagccctc ctcccgagtt
     3661 gcgggacgca gctcctccgg gagcgcatcg agccgcgtgc ccttgttcca atggcggagt
     3721 agcaacgccg ccgcgtcgtg cattcgatca ggcatcgtcg tcctccgaca ccagcaagag
     3781 cggtccacga gccgtgcgtg attccagcac ccggtgtcat tcagcctcgg aaaacccaaa
     3841 tacccgtccc accgccacac cccgggcaag gctggcctcc cactcggtct ccagcgcgag
     3901 cttcgcctcc gcgcccgcaa tgccgtcccg gatggaggcg ggcgagagcg cgaccaggcc
     3961 atcatcgtca ccgatgatga ggtcacctga gctgaccgtg gcaccaccga cctcgaccgg
     4021 gacattcacc gcgccgcgcc cagtcccgga cggcccacgc ggggtcacat ggcgggcgaa
     4081 caccggcagg tcggtccatc gggcgagggt tgcgacgtca cgcacggccc catcgcagac
     4141 gacgcccgcg ccacccagcc gtcgcacgtg gccgcacaga atctcgccaa tcatggcggt
     4201 gtgggcatca cctccggctg cgatgacgag tacgtccccc ggttggatca cctcgagtgc
     4261 gtgcagcact gcgccgaaat cgggcggatc gcacaaggct gtcaccgacc gtccgaagag
     4321 gcgcggctgc tgtcccggcg gcatcagcgg ccgaatcgcc gggtcgatct gccccggctc
     4381 gaggttgaga tcgaccacaa tggacacggg caccaaccgc catcgctcca gttccggcgg
     4441 taacgcgccc cgttccggcg catcccgctc gatccgcacc attcgctcac cccttccgcg
     4501 ccatcttggc tccacctgag gacaacttta ccgcaagcac aactgcctgc aatcgggtaa
     4561 agggatccac ccggcctcaa ttttccccgt ggcccatcag gtcgctgttg tcccaaagga
     4621 gctcaggctg gcttttgttg agcatgtccg gcgtggcgga agccgtctcg gagatctgtg
     4681 gagcttgcat cggcacttgt ggccccgacg gatgcagatt cctcgccgtt gtccgtggat
     4741 ggacgtaatc gcggacctcg ttcagcccgg cattccctca gttaccacca ctatgcacga
     4801 tggcagcgga gcggctgcca ggccgatgcc agcacccgga tctcgctcgc cctcaggctt
     4861 gccccgacat agtgagcccg accaccctgg catgttgggc aagtgcttcg cgcacatggt
     4921 cgtcccaata gacccaggta tgacctcccg aatgctcagt atagtgatgc tccagcccga
     4981 tcttctccaa atgattgtgg aagtcgcggt tttcctcgat caactggtcg tccacgccgc
     5041 aatcgaagga gataaccggc ttctgctcca gcgtcgagat ccgttcagca tacgcgatca
     5101 ggtccacatc ctctggttgc gcaagcatcg agaaatcgag atccgagtcc ctccagttga
     5161 acttcgagga gtgggaccag atcgaggcaa agcgatccgg gtacttgagt ccgagccaca
     5221 tcgctcccca accacccatc gagagcccac cgatcgccca tgggccgtcg gtcacgtgga
     5281 gatggcgacg gacatgatcg ctgatatccg tgatgatcag gtcctcgtag ttctgcctgc
     5341 caagccgacc cgcggccttc cagttcaggt atgcgctggt gccgccgtcc gggaagacga
     5401 tcaccatcgg ataggcattg gcgtgcaggg caacgttgga gcgctggatc caggtgttgc
     5461 agtcatcagt caagccatgc aactggaaga gcacgggaaa cggcccctcc cccgtctccg
     5521 gcagcagcac gttgtaactt gcgcgtttgc cgagggctct gctgaagaat tccattcggg
     5581 cgagatccat tcctcctcct tctcccctcc ctgtccggtc cacacgagtc acgattgccg
     5641 aaccgtcatg gcaaaccgca acatgcatcc aaacgccaca ccgcgcacct acgggctgag
     5701 acaagccgca tagaattgca cgcggaacat taataccccg ggagagtgcc acgataggcc
     5761 tcggcaaggt gaacaatgac ccgcatcgac attcaagagc gggaggatga ccccatcgag
     5821 gcaggcccat agatgggacc atgcatggag gaccagaccg cgccccaatg gcctgagcat
     5881 gataccggcg atgctgtgac tcttgtaaat gcgagcatcc tggtgcacca gttaagcgac
     5941 tgagagttat ggacctccat cgaaggtggc acagcgatgc ctcgcccctt cctcctcatg
     6001 tttgctccgg caactcagtc cctggctggc aactccggta ccgacgtatt cgccatgtcc
     6061 agggtcagca tctcgcgcgc gacctcgaga tcgatgagtg ttccccggtg gaggcggtcg
     6121 tagagctggt tggccttgtg ggtgatcagc ggcagatcgt gctgttcgac tttcgaaagg
     6181 gtctcacggg cagagggcac attccgggta gcgaggcggg aatagtcgct catcaccgca
     6241 ttggccggct cgagaaagcg gttgacgaaa tcccgtgcca gtggcagctc gtcgggatgg
     6301 tcgatgatcg ccgcaagcac ctgatccgac gctgcacagg cgtccagtgc tttatgccgg
     6361 acatccggat cagggatacg gcgggcgctt gaccgcatcc cgtctaccag cccagatgcg
     6421 cgatccaaaa tctcctggat ttccgtcgcc gccatcgtcc cggatgacgg tggctccccg
     6481 gtacgcatcc gcgcaagcgt cttgttgact tgccgctcct gccactcctc atcgaagaac
     6541 cgcttgtccc aaaagacgtt ccagccgtga atgaggaacc cgatgctcca gggaagcatc
     6601 ggccagtaga accacaagct gtcaggtccc gtaagcaggt tcataacgag aaaaaagagc
     6661 ccgacagcaa tgaatgcgcc agcatgctga acaaaccctc gcacatcctc cgcgtgtttt
     6721 tgcgcttgct ccctggcggc catcagctca tcatccatgc tccgctccac ctgtttggta
     6781 cctgcacttg tcccgctgcg cacccaccag cctgatcgag ctcggcatcg ggcccttccc
     6841 tcactccggt acattctcgg gaatcacttc ttcatccagg cccgcgggat caagcaggat
     6901 atagccaccg accgcgtcga tctccggagg acaggccgct gcaaggg
//
LOCUS       DASYWM010000098         4339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_247429, whole genome shotgun sequence.
ACCESSION   DASYWM010000098 DASYWM010000000
VERSION     DASYWM010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247429"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VGR29_03895"
     CDS             complement(<1..231)
                     /locus_tag="VGR29_03895"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEV2072764.1"
                     /translation="MMMDLEHLAWPGRHQPVIAANGIVATSQPLAAQAGLSILRAGGN
                     AVDAAIATVATLTVVEPGSTSIGGDAFALIWDG"
     gene            538..1089
                     /locus_tag="VGR29_03900"
     CDS             538..1089
                     /locus_tag="VGR29_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV2072765.1"
                     /translation="MARVIAGMTMSLDGFVSDRNGEVGRLYPDMEAMRQTGQLQEAIR
                     STGAVVMGRRSYQMGNGDFTGYEFQVPIFVLTHEAPVQVAKGENDKLTFTFVTEGIKR
                     VIDQAKAAAGDKDVTVVGGASTIRQCIKASLADEVHIDLRSILLGDGLRLFDRSGDPP
                     IELESIEVSESPGVTHLRYRIIK"
     gene            complement(1170..1451)
                     /locus_tag="VGR29_03905"
     CDS             complement(1170..1451)
                     /locus_tag="VGR29_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072766.1"
                     /translation="MDNRWIGWLAGAIFLIVIPWAHGITAEQIPMEPSSCMELASPAS
                     VDPGFARTVEDRSNDTEFSVVVEDQSHDEGFVPPMSPPCGATPFATPAP"
     gene            complement(1852..2934)
                     /locus_tag="VGR29_03910"
     CDS             complement(1852..2934)
                     /locus_tag="VGR29_03910"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2072767.1"
                     /translation="MVTEPALDMEKAFAFAFKVLGDFTACQMGTLNTIADRLGLFDRL
                     AESGSVTSAEFSEQAGIHERYAREWLSAMACHGYVSYDAASKRFSLPPEHAFCLANHD
                     SPVYLTGLFGMTPVTWSNVDLLTEAFRNGGGVPQERFGDEFWCGFERFTRTAFRNNLV
                     QDWIPAMPEVDARLRAGGSVADIGCGNGQALLYLAHGYPEATLVGYDNYAPAIEAANS
                     RAAEAGLGDRVHYEVLDVTHGIPGGYDLITTFDVVHDMPRPRPAMREIHGALNPGGTY
                     FVLEFNLSGDLQENIDHPLGIGAFGYSASTNYCMTQALAVDGEGIGTCMGEEKARELA
                     TEAGFSYFRRIDFPNSPFNLFYEIRA"
     gene            complement(3251..3559)
                     /locus_tag="VGR29_03915"
     CDS             complement(3251..3559)
                     /locus_tag="VGR29_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072768.1"
                     /translation="MEPSFIRPVMSNYFANRPERVPNLLSPDQDALSGSFGSSFSRRS
                     LVTRVRQASGALLIACGGYLSGAPRLAYTDPVILATAHMPATNWDTDDAEDAAGLAVK
                     "
     gene            complement(3730..>4339)
                     /locus_tag="VGR29_03920"
     CDS             complement(3730..>4339)
                     /locus_tag="VGR29_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2072769.1"
                     /translation="WLIGESAADLGRALHQEGELGRVATLMREGLLAFTEFRNLVEIH
                     ASLADLTLVALVADQPMRAAQLLGVARTFPTHTGRADMIARAVTYTHSRIGEAVFATE
                     AEAGGRLAWHDVLAEIDALVEAAFDATTAPPPTPTPACGLSRREIEVLQVVAEGYSSR
                     EIAETLFLSERTVETHIFHIYNKLNVSSRAAATAHAIRLGLL"
BASE COUNT          872 a         1256 c         1350 g          861 t
ORIGIN      
        1 gccatcccag atcagcgcga acgcgtcgcc gccgatgctt gtgctacccg gctcgaccac
       61 cgtgagcgtc gcgacggtcg cgatcgccgc gtcaacggca ttcccgcccg cacgaaggat
      121 ggagaggccc gcctgggccg ccagcggctg gcttgtggca actatgccgt tggctgcgat
      181 gaccggctga tgcctgccgg gccaggccag gtgttcgaga tccatcatca tgggttaatt
      241 ccgtttcctt accggcactg cctgccgtag tgataggtgg acgcattttc tgcgcggcca
      301 tcataccacc ggattttcct ccccggtgca tggccgtatc gttcttggcc gcaattgcgg
      361 tgaaatacgc cactctgcgt tgacaccaac tgcgatgtat gggtatccgg acgagggcct
      421 gtttgcatac tggtagccgg ggtcagatag cgcgactcgg gatggtccaa cctggggcga
      481 cgactggagt tgaataagaa gacgttgaaa taggcggtaa tgagaaggga gtggtggatg
      541 gcgcgggtga tcgcgggaat gacgatgtcg ttggacgggt ttgtcagcga ccggaatggc
      601 gaggtgggtc gtctctaccc ggacatggag gcgatgcggc aaaccgggca gttgcaagag
      661 gcgatccgat cgacgggggc ggtggtgatg ggccgccgtt cctaccagat gggaaacggt
      721 gacttcacag gatacgaatt tcaggtgccg atcttcgttc tcacccacga ggctccggtg
      781 caggtagcga agggagagaa cgacaagttg acctttacct ttgtcaccga gggtatcaag
      841 cgggtgatcg atcaggcgaa ggcggccgcg ggcgacaagg acgtcacggt ggttggcggc
      901 gcgagcacca tccgccagtg catcaaggcc agtttggccg acgaggtgca catcgacctc
      961 cgctcgatcc tcctcggcga tggtctccgg ttgttcgatc ggtcgggcga tccgccgatc
     1021 gaactggaat cgatcgaagt aagtgagtcg ccgggcgtta cgcacttgcg gtatcgcatc
     1081 atcaaatgag gggcaccgcc gacccgggaa tggaaacggg gctccatcgt ttcgcaaacc
     1141 cccggtttat ccggcaagcc atattgtcat cacggcgctg gagtggcaaa cggggtagca
     1201 ccgcaaggcg ggctcatcgg tgggacgaac ccctcgtcgt gggactgatc ttcaaccacc
     1261 acgctgaact ccgtgtcgtt cgagcgatcc tctaccgtac gggcaaagcc agggtcaact
     1321 gaggcgggag acgcgagttc catgcaggag gatggttcca tcggtatctg ttctgctgtg
     1381 atcccgtggg cccatgggat gacgatcagg aagatcgccc ctgctagcca tccgatccat
     1441 ctattatcca cggcccatac catcctttcc ggggtgaccg catgtagtgt gagacgttac
     1501 gtacatggaa cgcggtcatg acgtgacatg actactggtg tagacgcgct gtcatctgtg
     1561 gtggtttcac ctcttgaaga taggcgaaaa cgcgggctcg gcagacgcgt ccatcgtcgg
     1621 agacacgcat tccagccggc tctgggtgag caggccagcc attgggctgg cgtcaccaat
     1681 atggtaatgg ggtggatacg tgagggacac gacaacggcc cccgagagca gcaggagccg
     1741 tgtcgctggc caatatgact atgagccctt ctggcgacgc ctcgattggc gagcttccct
     1801 gacctgagct atcagtccaa tcgagccgcc accgatcaga cgacctacgc cctaggcccg
     1861 gatctcatag aagagattga acgggctgtt cgggaagtcg atgcgacgga agtagctgaa
     1921 cccggcctct gttgccagtt ctcgcgcttt ctcctcgccc atgcaggtcc cgataccctc
     1981 accatcaaca gccagtgcct gcgtcatgca gtaattcgtg cttgccgagt atccgaatgc
     2041 cccaatcccc aaggggtgat cgatgttctc ctgcaaatct ccggagaggt tgaactcgag
     2101 cacaaagtag gttcctccag gattgagcgc gccatgtatc tcccgcatgg cggggcgtgg
     2161 acgaggcata tcatggacaa cgtcaaatgt ggtgatgagg tcatagccgc cagggatgcc
     2221 atgcgtcacg tcgagaacct cgtagtgcac ccgatcgcct agtcctgcct cagcggcacg
     2281 ggaattagct gcctcgatgg caggcgcata gttgtcatag ccgacgagcg tggcctcggg
     2341 atagccatgc gcaaggtaga gcagggcctg accattgcca cagccgatgt cagcgaccga
     2401 cccgcctgcc cgcagcctgg catcaacctc cggcatggcc gggatccagt cctggaccaa
     2461 attattgcga aatgcggtcc gcgtgaatcg ttcgaagccg caccagaact catctccgaa
     2521 tcgctcctgt ggcacaccac cgccattccg gaacgcctcc gtcaacaggt cgacattcga
     2581 ccaggtaacg ggcgtcatgc cgaacaggcc tgtgaggtag acaggactat catggttcgc
     2641 taggcaaaag gcgtgctcgg gtgggaggct aaagcgcttg ctggcggcgt cgtaggagac
     2701 atagccatgg caggccattg cagagagcca ctcccgggcg tagcgctcat ggatgcccgc
     2761 ctgttccgag aattccgcgc tggttactga gccgctctct gcgagccggt cgaagaggcc
     2821 gaggcggtcg gcgattgtgt tgagtgtccc catctggcag gcggtaaaat ccccaagaac
     2881 cttgaacgca aatgcgaaag ctttttccat atcgagcgct ggttcagtaa ccattgtggt
     2941 tcctcctggt gaatcgtgga agagagggtt gaattgcggc tctgctcacg aagattgtga
     3001 gcaaggggaa ttacagcgcg gcttactacg tgaaggcggg atctcctttc ctggatggat
     3061 gtttatctat cgcatgcacg agatcgtcgg tgcgctggtg gtcccatgca tccatcgcga
     3121 tggctcctgt atggaagcgg gggtgcagac agagctcgta ctcatactca cccatgctcc
     3181 caggcgatcc tgccctggtt ctacaagggt ttattgcgac cgacaggagc cagggaggag
     3241 accgtccagg ttacttcact gccagaccag cagcatcctc ggcatcatcc gtgtcccagt
     3301 tggttgccgg catgtgcgcc gttgcgagga tgacggggtc ggtatatgcc agacgcggtg
     3361 ctccagagag atatccgccg caggcaatga gtagtgcccc tgatgcctgg cgaacccggg
     3421 tcaccagcga tcgtcttgag aacgaactgc caaagctgcc agacaacgcg tcctggtcag
     3481 ggctcaaaag atttggcaca cgttctgggc gattggcgaa ataattcgac atgacgggac
     3541 ggatgaagct gggttccatg acggtcatct cctgaaccag gtccccgcta cgtgcaccga
     3601 cgcggtgtgg aggacctgag agcaggatgg cggattgggc acgctccacc atcgtggaac
     3661 ctacgtattc ttcttgcgtc aggcgtctgg gaccagtacg tactttcccc aggcgaggaa
     3721 ctgccggggt tagagcaggc cgagtcgaat ggcatgagcc gtcgctgccg cgcgggaact
     3781 gacgttcagc ttgttgtaga tatggaagat gtgcgtctcg accgtgcgct cgctcaggaa
     3841 gagcgtctcg gcgatctcac gactggaata cccctccgcc accacttgca acacctcgat
     3901 ctcgcggcgg ctcagcccgc atgctggggt tggtgtcggc ggtggcgcgg ttgtcgcatc
     3961 aaacgcggcc tcgaccagcg catcaatctc ggccagcaca tcgtgccatg ccaacctccc
     4021 gccggcttcc gcttcggtgg caaacaccgc ttcaccaatg cgcgaatgag tatacgtaac
     4081 ggctcttgcg atcatgtccg cccgccccgt gtgggtggga aacgtccgcg cgacgccaag
     4141 cagttgcgcg gcccgcatag gttgatcagc gaccaatgcc acgagcgtga gatcggccag
     4201 ggatgcgtgg atctcgacca ggtttcggaa ttccgtgaac gcgagcagtc cctcgcgcat
     4261 cagcgttgcg acgcggccca gctcgccttc ctgatgtaat gcccgcccaa gatcggctgc
     4321 tgattctccg atcaaccac
//
LOCUS       DASYWM010000099        15295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_153337, whole genome shotgun sequence.
ACCESSION   DASYWM010000099 DASYWM010000000
VERSION     DASYWM010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15295
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153337"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(559..1350)
                     /locus_tag="VGR29_03925"
     CDS             complement(559..1350)
                     /locus_tag="VGR29_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072770.1"
                     /translation="MRTAVVHIRSERTLKAAASVLPSLIDHIDGSVAILPSSFDYRDP
                     VAEEVLTQLPQGSTREIVTPISGSAARRAFLLPTVLGDWRDVPINVPGVSVVSALMPK
                     AVASAEHRIIAVDVVEVSRHGPFVLDVPARYVHPRQRLRLVADRSRSSLVAEVAAALP
                     ISLSVVSLTLPEGTFLAATTDIIAAELVALALAERCIGSVRDFTGPWEDAVVQRATEL
                     ELGVLMPARIRLVADGRCLHEAWADTLQEHVRRRLGIPGTSSLRP"
     gene            complement(1412..3193)
                     /locus_tag="VGR29_03930"
     CDS             complement(1412..3193)
                     /locus_tag="VGR29_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV2072771.1"
                     /translation="MSEGRLSSVTQDFIFGTLATDELRLEDIRKRVRGLWHGSRIDPS
                     DPEPGDKVTLTVSAGGDISMREIGVHYTTDGSMPDSSSPALPMKVLHPRWDTLTWSYI
                     EERSAVIPPQDDGVLVRYRVRGVAESGAEIWADEVEDSGEPGLFAYCVDRDRVPHWLR
                     EAVIYQVFVDRFAPDPGKTFNDSGSLNDFWGGTLAGLLAHMDHIAELGATAIWLTPIF
                     PSPTHHGYDATDYTSVEPRLGTEEDLLAVVEAAHARRIRVLLDFVANHVSNQHPSFKH
                     AMMDPSSPEREWFAFNADGSYRSFFDVETMPQLAVDRDAVANHLIDAARHWLELGVDG
                     YRLDYANGPSLAFWSRFRRALRDTEPEVALIGEVVESADTMASYEGRLDGTLDFLLLQ
                     QLRAFLAFDLIGADEFWRFVSRHLTWFSENLSLPSFLDNHDMNRFLWVAGGDFRRLKL
                     AALVQFSLPHPPIIYYGTEVGLSQWHDLEHADGSRRMEESRTPMLWGEQQDVDLKSFY
                     RSLIFWRRRHGIHGIKPELVFANDSGLLILRLNAWFVVVNRSETDIVVDFREHGEVWL
                     ALATELDVQLHGSALQMTAMSGAVLRA"
     gene            complement(3186..4688)
                     /locus_tag="VGR29_03935"
     CDS             complement(3186..4688)
                     /locus_tag="VGR29_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HEV2072772.1"
                     /translation="MTESTRIERPTFGTEAGQVDTPQWTADTVFYQIFPDRFAKSDTL
                     VKPDGVEPWDTDPTTYGYKGGDLLGIVERLDYLVDLGVSGIYLNPIFQSASNHRYHTH
                     DYFKVDPLLGGDEAFDALMAACKSRGMRVVLDGVFNHASRGFFFFNDVLENGAASPWI
                     DWFHIKDDYPINAYDHDKPPGYGAWWGLHALPKLNTDNPQMREYLMQVGEYWIRKGID
                     GWRLDVPDEITTPGFWEEFRQRVRAINPEAYIVGEIWEVSTEYLAGQRFDAVMNYVFT
                     EAVLAFTGREHIVRKLQEDRAYKPWPPIDGVEYAHKIDLLLGVYDWNVHKAQMNLLDS
                     HDTARALTLASGDVRTLELAALLLFTFPGAPTVYYGDEIGLDGGLPDKWARKTFPWGD
                     ESQWNNALREHFQALIKLRQDLPSLRHGSYQGIAVTPESYAFIRRLEDETSLVALNTS
                     GYPDRLVIPQEYLLDLQIAVGDLPEVEATEDGMAISLPARSGGLWVATSV"
     gene            complement(4777..5682)
                     /locus_tag="VGR29_03940"
     CDS             complement(4777..5682)
                     /locus_tag="VGR29_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2072773.1"
                     /translation="MAVHAPLAPTAVSDIKEKASWKESSKFQRHAEVWVIRLIIWAII
                     AMVMFPVVYVVLYSIKGGGSSLYSTTFIPAVRTLDNYRRLLSTESNFPGWVLNSLILG
                     IASGAITVIFATFAGYAFSRMRFPGRRYGILTLLIIQMLPVNMAAVAYYRMLQLVGLT
                     NTRIGLILIFGFGGSAWAVWLMKNFIDSIPKDLDEASYLDGGNHWTTFWRVIFPLVQP
                     MLVAQFIFGFIGVYNEYILTSLLIFDPNLYPLGVGVRTFSTAFATSWTLFCAAAVLGS
                     IPILIVFFLAQRFLVEGLTKGAVKG"
     gene            complement(5682..6662)
                     /locus_tag="VGR29_03945"
     CDS             complement(5682..6662)
                     /locus_tag="VGR29_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2072774.1"
                     /translation="MSAQSQQFGTQAENAQSGHPFKGPEPRRRKIPVAYLYLLPVILS
                     ALIFTIGPFVYTFYISFTNYHLIYHFNEFEWIGLANYTRALGEGGEFVPVLRWTIIWM
                     FLTTVLNVGGGMFLALMLNHERLVERNLYRTILILPWALPNILLIQVWAGLYNAQGPI
                     NLILGRIGFDENVRWLLEDTPARTALLLTNLWLSYPFFMTVGLAALQAIPRDLYEVAD
                     LDGAGSWARFRDITFPFMLSAITPLLLTQLAFQFNNAGVIILLTNGLPQAFPGSQYGV
                     TDTLATFAYKLIYAERDMGYAAAYGVFTFLIIAVFIVLSARLTGSFKEVD"
     gene            complement(6759..8144)
                     /locus_tag="VGR29_03950"
     CDS             complement(6759..8144)
                     /locus_tag="VGR29_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2072775.1"
                     /translation="MTHDIRIRRLFEQVAEGKISRRDALYRAAVLGASATAIGATGGS
                     AMAQDPSLVASPGASPAAVSCYMAQGPQVEQLVLWTRSSADTSPNEWNALVAATERYS
                     ELTGTPVELVTVPDADFRTKLSQAAPGGDGPDVFGPVAHDWIGEVALQQIAMPWEEGE
                     LHGLEDIPQSAINAVTYEGQIYGYPIFSETLGLYYNRDIISEPPKTWEELVQKAKDAT
                     EGDQFGFAFEILAPYYQGAFFHAYDSYIFKNNDGTLDFNDIGLNNEGGVEAAKALRDM
                     YWNQEPPMPEAILDQANAGAYLDGLFESGQLGMRISGPWLAPALRDAGINFGVAPLPT
                     GPNGELLQPFSGIQVFEVNAYSDHIEAAKDLVNFLGCEGVSMMIEGFNKPPVRNSLKE
                     KAIDINPDLAAWMEAAEQAVPMPNIPQMAQVWQPWTDAMVGIITNNVSDEEVQALLDA
                     AVEQIKANIQQ"
     gene            8431..9315
                     /locus_tag="VGR29_03955"
     CDS             8431..9315
                     /locus_tag="VGR29_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072776.1"
                     /translation="MNSPRYERIDVPASFSLRETCAPVWWIGDRSPRHAWIEDTLVVI
                     EASGDHDVYREVTQPIPGVLRISTAVSDVHHATWARRVLGTGVNMPSWSDPVLEKLSG
                     TFPGMRPYSDGSLFAGIITAIIGQSVSLASAAAAQRKLAMAFHPGVSSRRRMFFPLPR
                     PAQLADASLDLIRSSGVTWKRAEALQFIAREQLAGRLPTDDQATSDQDEVTRALLALP
                     VVGKWTAQSVLLWGVGAMDAHPTGDVALLNAARKAYARPTMTMRELDTLSEEWRPNRG
                     MAARLLWANLFGTPPAAK"
     gene            complement(9355..9774)
                     /locus_tag="VGR29_03960"
     CDS             complement(9355..9774)
                     /locus_tag="VGR29_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072777.1"
                     /translation="MNAEKRYGWQRETAYMSRERRRTIAVDFDGVIHRYSKGWSGGSI
                     YDPPVEGAREALARIHVRYKIVIFTTRVNPQMHGAESQMDAVHAWLETHGFRRGQHFD
                     EITHVKPPALAYIDDRAIRFTDWESTLDELGQLYRLS"
     gene            complement(9904..10356)
                     /locus_tag="VGR29_03965"
     CDS             complement(9904..10356)
                     /locus_tag="VGR29_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV2072778.1"
                     /translation="MTTLRNGLGLLLRASVLLMLAASLVACGDEDEVATDQDAVPAAS
                     AEGVDELPEGLEAAILEIEDGEFDEDELTLQQGEPTVLQIVNGDDRPYEFLIEDLVAV
                     QSIPAGTTAQIEFTTPNPGDYAGLLLDPEDESVVSEVTVTVQDAGGLQ"
     gene            10925..11725
                     /locus_tag="VGR29_03970"
     CDS             10925..11725
                     /locus_tag="VGR29_03970"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEV2072779.1"
                     /translation="MAEAKESPVESNPVASSEVRRVAQGPVMPIGGAEDKDGDGDEAI
                     LQRFVDLAGGKKARIVVVPTASEHQEEMGQKYTDIFTALGAKSVEVLNIQTREETNAD
                     PAIEMLEKSTGIFITGGAQSRLVTLLSGTLAMECIRERNARGVVVAGTSAGASIVASH
                     LMLGGTGLAGNSGSSAARKHMVEMAAGFGPLQDIIIDQHFSERGRMGRLLSVYAANPG
                     LLSVGLDEDTAVVIDKEGIMEVLGTGMVTVVNGRNAVSDTLSARSVRC"
     gene            11904..12914
                     /locus_tag="VGR29_03975"
     CDS             11904..12914
                     /locus_tag="VGR29_03975"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV2072780.1"
                     /translation="MLAEVTRGPLVESTHQGNVAVVDAEGRLIASGGDVESRSFFRSS
                     AKPFQGVPLVISGTADAYGFTTEELALACASHNATERHQAIVSSMLKKAGFLESDLRC
                     GFSPPFDKEENARITLGLKEPTQIRCECSGKHAGMLAVCRHNGWPTDNYTALDHPLQQ
                     EILSVVGAACNVPSSAFEIAIDGCTLPTFAAPIRNFALAFAVLADPEGACWEGDSAHR
                     AALVRLREAFILHPELVSGEGEIDTTIMRTTQGRVVAKLGAEGLLCMAVPEHRLGVAI
                     SDSGGSTRSLGPAAISVLEELKLEDESTVASLRQQLCPPIQNFAGQPVGETRPTLKLE
                     RA"
     gene            13089..>15295
                     /gene="cphA"
                     /locus_tag="VGR29_03980"
     CDS             13089..>15295
                     /gene="cphA"
                     /locus_tag="VGR29_03980"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEV2072781.1"
                     /translation="MLKIQNTRVLRGPNVWARVPVIHLVVDIGELEERPSNTIPGFVS
                     RLVQAVPSLEEHECSLGRRGGFIERLRDGTWMGHVMEHVALEIQRLAGIHVSRGKTRR
                     TGERGIYNVVYAYRQEDVGLAAGQLSARFLNHLIYDVEPGFDLVRELEERVVRLAERL
                     AYGPSTLEIVQEAERRGIPVMRLNSNQSLVQLGHGRYQRRIWATRTSTTSGVAMTIAG
                     NKELTNRLLRDAGIPSPRGVVAGSAQEAVREVNRLGYPVVLKPVDGNHGRGVCLNLSN
                     RTEVEESFPLPKAASREGLVLVEPMVPGKDFRVVVVGSEVVAVAERVPACVVGDGQHS
                     VRELVDLTNVDPRRGVGHEKILTRIHVDSQTHEVLSGQGLTLDDIPADRQCVQLKLTA
                     NMNTGGTSIDRTDEIHPDNVEIGLEAAMVVGLDVAGIDFITPDISRSVHEAGGAIVEI
                     NAAPGFRMHTHPTEGLPRQVGHTVVDMLFPNGTPASIPIIAVTGTNGKTTTTRMIAHI
                     MQKDGRTVGMTTSDGIYVNGTQIAAGDKAGPDSARMVLRNPRVDMAVLETARGGILRS
                     GLGFNRCNVAVVTNVASDHLGMKGVDTIEDLAKVKTALPASVLREGTSVLNADNQWTV
                     DMARIARGEIMYFSMDENNALTRDHVRKQGRAVVLRETPRGGMLTLVEPKRETNILLA
                     RDIPATFNERVRGNIANALAVTAAAIGAGVPLACIRDSLRTFSTAFWQTPGRF"
BASE COUNT         3281 a         4562 c         4525 g         2927 t
ORIGIN      
        1 ctggtatcca gcagatggag caatccttgg taatgaccat caggtggagg ggatccaccc
       61 tgtgtcaacg gactctcccc aactcaagca ggcccgcggc agggccggac aatctgctcg
      121 tatagcggat gttcaatggg acgcaagaag cccaccactt atcatcccgt tgagctggga
      181 acacatggcg ggctgcggca gtacctggga gccgcatccg cggctccttc ggatccatta
      241 gataaccgct gtcatcttga ggcgaatatg acgtctacct gcggcgtgga ttctcgacac
      301 ggacaggaac aagcactggc tggggtcgcc gaagtagggt ttcgatccac tcacgaatga
      361 gcttcagcaa cacagtgttc acgatataac tcctgcaact tggggtcgtc cttgagcacg
      421 acccccggct ctgttacgtt gccgatgctc ttcaccaagc tgatggtgcg tggctaccat
      481 gctaccaata aaatcgctgc tcgtcacggc gattctcgga aacgtgttgt atccctgcaa
      541 tgcgcgtacc atagctgtct acggacgcag gcttgacgtg cccggaatgc ccaatcgcct
      601 ccgcacatgc tcctgcaatg tatccgccca ggcctcatgc aggcaccgtc cgtcggcaac
      661 gagacggatc cgggccggca tcagcacacc caactccaac tctgtggcac gttgcacaac
      721 ggcgtcctcc cagggcccag taaagtcgcg aaccgaaccg atacatcgct ccgccagcgc
      781 caaagcaacc agctcggccg cgatgatatc cgttgtagca gcgaggaacg ttccctcggg
      841 taaggtcagg ctcacgacgg acaggctgat ggggagcgcc gcagctacct cagcgacaag
      901 actggatcgg gagcgatccg caacgagccg cagtcgctgt ctaggatgca cataccgtgc
      961 agggacatca aggacaaacg gcccgtgccg cgaaacctca acaacgtcga ccgcgatgat
     1021 ccgatgctcc gcagatgcaa ccgctttcgg catcaacgct gacacaacgc tcactcctgg
     1081 gacgttgatc ggtacatcgc gccagtcacc gaggacggta ggcaagagaa aagcccgccg
     1141 ggcggcggag ccactgatgg gcgtcacaat ctctcgtgta ctgccttggg gcagttgagt
     1201 aagtacctcc tctgccacgg gatcgcggta gtcaaacgac gaaggcagga tcgccaccga
     1261 accatcgatg tgatcgatca atgacggcag cacggatgct gccgctttga gggtgcgctc
     1321 gctccgaata tggaccaccg ccgttcgcac tgaatgcctc catcgctgcc gatgcggtct
     1381 gctttcacca agtatgcgtc tttgatccga atcaggcccg aagcaccgct ccgctcatcg
     1441 cggtcatctg cagggccgac ccgtgcagct gaacgtcaag ctcagtcgcg agcgccagcc
     1501 aaacctcgcc gtgttcgcgg aagtcgacga cgatatcggt ctcactacgg ttgacaacca
     1561 caaaccacgc gttgagccgg aggatcagga gccctgagtc gttagcgaag actagctccg
     1621 gcttgatgcc atggattcca tggcgcctgc gccagaagat gaggcttcga tagaacgact
     1681 tcaggtcaac atcttgctgc tctccccata gcatgggcgt ccgcgattcc tccatgcgac
     1741 gagaaccatc cgcatgctcg aggtcatgcc actggctgag gcccacctcc gttccgtagt
     1801 agatgatggg cggatgtggc aaggagaact gcacaagtgc ggccagcttc agccggcgga
     1861 agtccccacc ggcgacccag agaaaccgat tcatgtcgtg gttgtctaga aaactgggca
     1921 gtgaaaggtt ttccgagaac caggtgagat gccggctgac gaacctccag aattcgtcag
     1981 caccaatgag atcgaacgcg aggaatgcac ggagttgctg caacagcagg aagtccagcg
     2041 tcccgtccag acgcccctca taggacgcca tcgtgtccgc gctttcaacc acctcgccaa
     2101 tgagcgcgac ctcgggctcg gtatccctga gggcccggcg aaatcgggac caaaaggcca
     2161 gcgatgggcc atttgcatag tccagacgat agccatcgac accgagttct agccagtgcc
     2221 tcgcggcatc gatcaggtga ttggctacag catctcgatc gaccgccagc tgcggcatcg
     2281 tctccacgtc aaagaaggac ctgtaggaac catccgcatt gaaggcgaac cattcacgct
     2341 cgggggaaga gggatccatc atggcatgct tgaacgacgg gtgctggtta gacacgtgat
     2401 tcgcgacaaa atcgagtagc acgcgtattc tccgcgcgtg tgccgcttcg acgacggcaa
     2461 ggagatcctc ttcagttccc agtcgcggct caacagaggt gtaatcggtc gcgtcgtagc
     2521 cgtggtgggt gggggaggga aagatcggag tcaaccagat ggcggtcgcg ccaagctcgg
     2581 caatgtggtc catgtgcgcg aggagcccgg ccaacgtgcc gccccagaaa tcgttgaggc
     2641 tcccggagtc gttgaacgtc ttgcccggat cgggtgcgaa tcgatcaaca aaaacctggt
     2701 agattacggc ctcgcgcagc cagtgcggca ccctgtcgcg atccacgcaa taggcaaata
     2761 gacccggttc accggaatcc tcgacttcat ccgcccagat ctccgcgccg ctctccgcaa
     2821 caccgcggac gcgatagcga acgagtacgc catcgtcctg aggggggatg acggcagacc
     2881 gctcctcaat gtagctccag gtcagcgtgt cccacctcgg atggagaacc ttcattggga
     2941 gagcggggga gctggaatcg ggcattgatc cgtcggtggt gtaatggacc ccaatctccc
     3001 gcatcgagat atcgccgcca gcgcttacgg taagggttac cttgtctccg ggctcggggt
     3061 cagaggggtc gatcctgctc ccatgccaaa gaccgcggac gcgcttccgg atatcctcaa
     3121 gccgcaattc gtcggtggcc agtgtcccga agatgaagtc ctgtgtgacc gacgagaggc
     3181 gtccctcaga cactggtcgc cacccagagg ccgccggacc tcgctggcaa cgagatcgcc
     3241 atgccgtcct cggtagcctc cacctcgggt agatcgccaa cagcgatctg gagatccagc
     3301 aagtattcct gcgggatgac gagccggtcc ggatagccac tggtgttcag cgccacgagg
     3361 gaagtctcgt cctcaagtcg ccgtatgaag gcatagcttt cgggcgtcac tgcgatcccc
     3421 tgataggacc cgtgacgcag cgacgggagg tcttgccgaa gcttgatcag tgcctggaag
     3481 tgctcgcgga gagcgttgtt ccactgcgat tcgtctcccc aggggaaggt cttgcgggcc
     3541 cacttgtccg gaagcccacc gtccaggcct atctcatcgc cgtaatagac tgtcggcgca
     3601 ccagggaagg tgaagagcag gagcgccgcc agttcaagcg tacggacatc accactggca
     3661 agtgtcagcg cacgggccgt atcgtggctg tccagcagat tcatctgggc cttgtggacg
     3721 ttccagtcgt agaccccaag cagcagatcg attttgtgcg cgtactcgac accatcgatg
     3781 ggaggccacg gtttgtaggc ccggtcctcc tgcagcttcc tgacgatgtg ctcgcgacca
     3841 gtaaaggcaa ggacggcctc ggtaaagaca tagttcatca ccgcatcgaa gcgctgaccg
     3901 gccaggtact cggtgctgac ctcccagatc tccccgacaa tgtatgcctc cgggttgatg
     3961 gcccggaccc gctgtcgaaa ctcctcccaa aagccgggcg tcgtgatctc atccggaacg
     4021 tcaagccgcc agccgtcaat tcccttacgg atccagtact cccccacctg catcaggtat
     4081 tcgcgcatct ggggattgtc ggtgttgagc ttcggcaggg catggagtcc ccaccaggcg
     4141 ccataaccgg gcggcttatc gtggtcgtag gcgttgatgg gatagtcgtc ctttatgtgg
     4201 aaccagtcaa tccagggcga agcagctccg ttttccaaca cgtcgttgaa aaagaagaat
     4261 ccgcggctcg cgtggttgaa gacgccatca agcacgacgc gcatgccgcg gctcttgcag
     4321 gcggccatca gcgcatcgaa tgcctcgtca ccacccagca gcggatcgac cttgaagtaa
     4381 tcgtgcgtgt ggtaacggtg atttgaggcc gattggaaga tcggattcag gtagattccc
     4441 gacacaccaa gatccactag atagtcgagc cgctcgacaa tgcccagtaa gtcaccaccc
     4501 ttgtagccat acgttgtggg atccgtatcc cacggctcga cgccatcggg tttgaccagc
     4561 gtatcgctct tcgcaaagcg gtccgggaag atttggtaga agacggtgtc agctgtccat
     4621 tggggtgtgt caacctggcc tgcctctgtt ccaaaggtgg gccgttcgat tcgtgtcgat
     4681 tcagtcacga agtctttcct taccgttcct gccagtttgc cgtcgatctt cataatcgat
     4741 tcggacgatg gtgccggcaa gcagacctgc gtgatcctac cccttcaccg cacccttcgt
     4801 gaggccctcg acgaggaacc gctgtgcaag gaagaagacg atcaggatgg gaattgaacc
     4861 gagcactgcg gcggcacaga agagcgtcca tgacgttgcg aaggccgtgg aaaaggttcg
     4921 gactccgaca ccgagcggat agaggttcgg atcaaagatc agcagcgacg tcaggatgta
     4981 ttcgttgtac acaccgatga agccgaagat aaactgcgcc accagcatgg gttgtaccag
     5041 cgggaagatc acccgccaga aggtggtcca gtgattaccc ccatccagat aggatgcctc
     5101 gtccaggtcc ttcggaatcg agtcgatgaa gttcttcatc agccacaccg cccacgcgga
     5161 accgccaaaa ccgaagatca ggatgagtcc gatgcgggta ttggtcaggc ccaccagttg
     5221 gagcatacgg taataggcga ccgcggccat attcacgggc agcatctgga taataagcaa
     5281 cgtcaggatg ccatagcgac gtcccgggaa gcgcatgcgg gagaaggcat aacccgcgaa
     5341 ggttgcaaag atcacggtga ttgcgcccga cgcgataccg agaatcagtg aattaaggac
     5401 ccagcccggg aagttactct cagtagaaag cagccgtcgg tagttatcca gcgttcggac
     5461 cgccgggatg aacgtggtgc tgtagagcga tgaaccgccg cccttgatgc tgtagagcac
     5521 gacatagaca accgggaaca tcaccatagc gatgatggcc catatgatga gccggatgac
     5581 ccagacctca gcatgtcgct gaaatttgga actctccttc cagcttgcct tctccttgat
     5641 gtcgctgacg gcagtcggtg caagcggcgc gtgtacggcc attagtcaac ctccttgaac
     5701 gatcccgtga ggcgcgcgct caggacaata aacacggcga tgatgaggaa ggtaaagacg
     5761 ccgtacgcgg cggcatagcc catgtcccgc tcggcgtaaa taagtttgta ggcaaaggtc
     5821 gcgagcgtat cggtcacacc atactgggag cccggaaatg cctgtggcag tccgttcgtg
     5881 aggaggatga tcactcctgc gttgttgaat tggaacgcaa gctgggtgag caggagcggg
     5941 gtaatggccg agagcatgaa ggggaaggtg atatctcgaa accgtgccca acttccggcc
     6001 ccgtcgaggt ctgctacctc gtagagatcc ctggggatcg cctgcaatgc cgccaggccg
     6061 accgtcatga aaaagggata ggagagccag aggttagtga gcagcagggc cgttcgggca
     6121 ggggtgtcct ccagcagcca tcgcacattt tcgtcgaaac cgatacgacc gagaatcagg
     6181 ttgattggac cctgcgcatt gtagaggccc gcccacacct ggatgagaag gatattcggc
     6241 agcgcccagg gaaggataag gatcgtgcgg tagagatttc gctcaacaag ccgctcatgg
     6301 ttgagcatca gggcgaggaa catccctcca ccgacattca gaacggtcgt gaggaacatc
     6361 cagatgatgg tccaccgaag taccggtacg aactcaccgc cttcgcccag ggcgcgggtg
     6421 tagttggcaa ggccgatcca ctcgaattcg ttgaaatgat agatcaggtg atagttggtg
     6481 aacgagatat agaacgtgta gacaaagggc ccgattgtga agatcagcgc cgacaggatg
     6541 acaggcaaca ggtacaggta cgccaccggg atcttgcgtc gccgaggctc tggccccttg
     6601 aaaggatggc cggactgggc attttcggct tgcgtgccga actgttggct ctgagcactc
     6661 attgcttccc ccacgcacaa ccaaccggga gttgaaaatg ccgggaggcg atgctgaacg
     6721 cacacgcctc ccggcagagt caagccagca caccagcctt actgctgaat gttggccttg
     6781 atctgctcga cggcggcgtc cagtaatgcc tggacctcct cgtccgagac gttatttgtg
     6841 atgataccga ccatggcgtc ggtccacggc tgccagacct gtgccatctg ggggatgtta
     6901 ggcatcggca ccgcctgttc tgcagcctcc atccatgcag cgagatcggg attgatatca
     6961 atcgctttct ctttgaggga attgcggacc ggaggcttgt tgaagccctc aatcatcatc
     7021 gaaactccct cgcatcccag gaagtttacg aggtcctttg cagcttctat gtggtcactg
     7081 tatgcattga cctcgaagac ctggattccg gagaagggct gcaacagttc cccattcggg
     7141 ccagtcggca acggtgccac accaaagttg atcccagcgt cgcgcagggc cggcgccagc
     7201 cacgggccac tgatacgcat accaagctga ccggactcaa aaagaccgtc gaggtaagcg
     7261 cccgcgttcg cttgatccag aatcgcctca ggcatcggcg gctcctggtt ccagtacatg
     7321 tcccgaagcg ccttggccgc ttcgacacca ccctcgttgt tcaggccgat gtcattgaag
     7381 tccagggtac cgtcgttgtt cttgaagata tacgaatcgt aggcgtgaaa gaacgcgccc
     7441 tggtagtacg gcgccaatat ctcgaacgcg aacccgaact ggtcaccctc ggtagcatcc
     7501 ttcgccttct ggaccagttc ttcccacgtc ttgggcggct cggagatgat gtccctgttg
     7561 tagtaaagac ccagcgtctc cgagaagatt ggatagccgt aaatctggcc ctcgtacgtc
     7621 acggcgttaa tggcggactg gggaatatcc tcaaggccgt gaagctcacc ttcctcccat
     7681 ggcatggcga tctgctggag ggcgacttcg ccgatccagt catgtgccac cggcccgaag
     7741 acatccgggc catctccgcc gggggcagct tggctcagct tcgtacggaa atcggcatcg
     7801 ggcacggtta caagctccac cggagtgccg gtaagctcgc tatatcgctc ggttgcagcg
     7861 acgagagcat tccactcgtt cggggaggta tccgcggacg agcgggtcca gagaaccagt
     7921 tgctcaacct ggggcccctg ggccatatag catgaaactg cggcggggga agcacccgga
     7981 gaagccacca ggctggggtc ctgagccatt gccgaaccgc cggtagcacc gatggcagtg
     8041 gccgaggcgc caaggaccgc ggctcgatag agcgcatctc ggcgcgaaat cttgccctcc
     8101 gcaacttgct cgaacaatct cctgatccga atatcatgag tcatgcgtac cgattccttt
     8161 ctttacaatg cggcaacgcc acattgcgca ccgcactacc actgatctaa tcaacgtgcc
     8221 gaattcatgt cattttccgt gccatccaat caagcaatgg acgacaaccg ttttcgatgg
     8281 acaggcgaac catgcgcatc tcatcgaatc tcgttacaac agtgcaactg atcaccatac
     8341 gcgttgccgc gtggtgtcgc gagcgttccc gctttcgggt gagttggagc accagttaat
     8401 gagaagttcg aaaaaacgag ccatgaccga atgaacagtc caagatacga gcgcatcgac
     8461 gtgccggcct cgttttccct tagggaaacc tgcgcgccgg tttggtggat aggcgaccgt
     8521 tccccccgac atgcctggat tgaggacacg ctggtcgtta tcgaggcgtc aggtgatcat
     8581 gatgtgtatc gcgaagtaac acaaccaata ccgggtgtac tgcggatatc caccgcagtt
     8641 agtgatgtgc accacgcaac atgggcgcgc cgcgtgctcg gaacaggggt gaacatgccg
     8701 agttggagcg atccggttct ggagaagctg agcggaacgt tccccggcat gcgaccgtac
     8761 agcgatggct ctctcttcgc gggcatcatc accgcgatta tcggacaaag cgtctcgctg
     8821 gcatccgccg ccgcggccca acgcaagctc gccatggcct ttcatccagg cgtgagcagc
     8881 cgtcgccgga tgttctttcc attgcccagg ccggcccagc tcgccgacgc atcactcgat
     8941 ctcattcgct catctggtgt gacatggaag cgggcagaag cactgcagtt tatcgccagg
     9001 gagcagctgg cgggtcggtt gcctacggac gaccaggcaa cgtcagatca ggatgaggtg
     9061 acacgggcct tactcgcatt gcccgtggtg gggaagtgga cggcccagtc ggtcctgctg
     9121 tggggcgtcg gcgctatgga cgcgcatccg accggtgatg tcgcgctgct caacgccgcg
     9181 aggaaggcct acgctcgacc cacgatgacg atgagggagc tcgataccct gtctgaagag
     9241 tggcgcccga accgcggaat ggcagcgagg ctcctctggg ccaacctgtt tgggaccccg
     9301 ccggctgcga aatgaacggc aggggcctcc gcgaagtgaa caaccggtag tgcgtcagga
     9361 gaggcgatag agctgcccga gctcatccag ggtcgattcc cagtcggtga accggatcgc
     9421 cctgtcatca atgtaggcaa gcgccggtgg cttgacgtgg gtgatctcgt cgaaatgctg
     9481 cccgcgtcga aacccgtgcg tctccaacca tgcatggacc gcgtccatct gactttcggc
     9541 gccgtgcatc tgcggattca ccctggtcgt gaagatcact atcttgtacc gaacgtggat
     9601 tcgggccaat gcttctcgtg cgccctcgac cggagggtca tagatagatc caccggacca
     9661 tcccttgcta tatcggtgga tcaccccgtc aaaatctact gcaatcgtgc gccttcgctc
     9721 ccggctcata taagcagtct cgcgttgcca accgtagcgc ttttcggcat tcattctcgt
     9781 gtagcgcacc ggaacctgga ccgcgaatcg ccaagttacg gccgaccggc cgagaacacc
     9841 tgtgcggcga gcgcgccgac cacgcctggc atcatggtgt ctccgaacag tgggctgaaa
     9901 cccctattgc agcccccctg catcctgaac cgtcaccgtc acctcggaga ccacgctctc
     9961 atcctccggg tcgaggagta ggccggcata gtcgccagga ttgggagtgg tgaattcgat
    10021 ctgggctgtt gtcccagcag gaatcgactg cactgcaacc agatcctcga tcaaaaattc
    10081 gtaggggcga tcgtctccat tgactatctg caaaaccgtc ggttctccct gctggagagt
    10141 gagttcgtcc tcgtcaaact ctccatcctc aatctccaat atcgctgcct ctaatccttc
    10201 gggcagttca tcgacaccct cggcggacgc agcgggcacc gcatcctggt ccgtggctac
    10261 ctcatcctcg tctccacaag caacgagtga agcagcgagc ataaggagca ccgaagcccg
    10321 aaggagcaaa cccaatccat tcctgagtgt tgtcatagcg ttgtcaaccg tcccttcttt
    10381 cgcaccaact tgtccacaaa tcatatgagc agagtgtcag cgccgcatag ccttcgtaat
    10441 tgacggccaa aacgatccgc atcagcccat tgagaacgta ccgccttcac caccgctctc
    10501 aaatctcgaa tccactcctt cgaggatcga tcctctattc ccggcagctg cataatcaag
    10561 gtgattccac tggcggacat ggctcgctat gggttcgagc tttggaccga cgtcgacggc
    10621 agaggcaatc ctgctctcga gaacgtggac ggccacacgc cgaggcactg cgggtaggtc
    10681 ttccgacgaa ccgggaccat cctgcggagc ggcgcgcaaa gtcagtgcca gtgttcggcg
    10741 gacgccatag aagcgtgaca cagacagccg aaagtcaggc aggcaagtga aggcgggatc
    10801 gaggaagccg aatgggatac ctattgcatt aggggttgca gaacgaccgg acagcacgac
    10861 gctgctgtcc cgtaaccatg ttctttagcg tcaggcggtt ttgaggggga caacggagcg
    10921 ggtcatggct gaagctaaag aatctccagt ggaatcgaat ccggtcgctt catcggaagt
    10981 gcggcgagta gctcaaggtc cagtgatgcc tatcggcgga gcagaggaca aggacggcga
    11041 cggggatgag gcaatcctgc agcgttttgt ggaccttgca gggggcaaga aagcgcgcat
    11101 cgttgtggtt ccgacggcgt cggagcatca ggaggagatg gggcagaaat acaccgacat
    11161 cttcacagca cttggcgcga agtcggtcga ggtgctcaac atccagacac gcgaagaaac
    11221 caacgctgat cctgcgatcg agatgcttga gaaatccacg ggcatcttca tcaccggagg
    11281 agcacaatcg cgtctcgtca cactcctctc cggcacgttg gccatggagt gtatccgtga
    11341 acgcaacgcg cggggtgtcg tggtagcggg aacgagtgcc ggtgcatcaa tcgttgccag
    11401 ccacctcatg ctgggtggaa ccgggcttgc cggtaacagt ggcagttccg ctgccaggaa
    11461 acatatggtc gagatggctg cggggttcgg cccgctccag gatatcatca tcgaccagca
    11521 tttcagcgag cgaggccgca tgggccggct cctttccgtg tacgcagcga atcctggtct
    11581 gctcagcgtc ggccttgacg aggacacggc agtcgtaatc gacaaggagg gcatcatgga
    11641 ggttctcggg accggcatgg tgacggttgt caacggccgc aatgccgtgt ccgacacttt
    11701 gagcgcaagg tcggtgaggt gttgaccgtc gccaactccc atctctttgt ccttggtccc
    11761 ggtcggcgtt tcgacctcaa cactcgacaa gccatcatgg acgaggtcat cggcagctag
    11821 gccgaccagg cgcaaccaat ccggccgctc aggccacacc ctcaaccact tacttgattg
    11881 tctggagacc caccgtggcg acgatgcttg ccgaagtgac ccgtggtccg cttgtcgaga
    11941 gtacccatca ggggaatgtt gccgtcgtcg acgccgaagg caggctcatt gcctcaggtg
    12001 gcgacgtcga atcacgctcc ttcttccggt cgtccgcgaa gccgttccag ggcgtcccgc
    12061 tcgtcataag tggcacggcc gacgcatacg gcttcacgac cgaggaactc gctctcgcct
    12121 gtgcctcgca caatgccaca gaacgacatc aggcgatcgt ctcctcgatg ctgaagaagg
    12181 ccggcttcct ggagtcagac ctgcgctgcg gattctcgcc acccttcgat aaggaggaga
    12241 acgctcggat cacgctcggc ctgaaagagc cgacgcagat tcgatgcgag tgttcgggga
    12301 aacacgctgg catgctggcc gtctgccgcc acaacggttg gccaaccgac aattacaccg
    12361 ccctcgatca tcctttgcag caggagatcc tctcggttgt tggagcagcc tgcaacgtac
    12421 cgagttcagc attcgagata gctatcgatg gttgcaccct gcccaccttc gctgctccga
    12481 ttcggaactt cgctctcgcc tttgcagtcc tggcagaccc ggaaggtgcc tgctgggaag
    12541 gtgattccgc tcaccgggct gctctggtcc ggttgcgtga ggcgttcatt ctccacccgg
    12601 agctcgtttc cggcgagggc gagatcgata ccaccatcat gcgcacgact caagggaggg
    12661 tggtcgcaaa gctgggtgct gaggggctgc tctgcatggc ggtacctgag caccggcttg
    12721 gagttgccat cagtgactca ggtggctcca cacgcagtct tggtcccgct gccatctcgg
    12781 tgctggagga attgaaactg gaggacgaga gcaccgtcgc cagccttcgg cagcagcttt
    12841 gtccgcctat acagaacttt gcgggccaac cggtaggcga gactcgtcca accctcaaac
    12901 ttgaacgcgc ctgagcctcc ggcaagggcg gagacagccc tgcccgaaat cggattgact
    12961 ccataatttt ggagaaaacg cgttgatgag cgcaaatacc atcagtggca atcgtcggga
    13021 tcgacaagcc aagaacgccc gacgggagca cattgcccgg tgcccgctca ccgaaaggtt
    13081 gaacagtcat gcttaagatt cagaacacca gggtactccg gggaccgaac gtctgggcac
    13141 gggtgcccgt gatccacctc gtcgtcgaca tcggtgaact cgaggagcgg ccctccaata
    13201 ccatcccagg attcgttagc cgtcttgtgc aagccgtacc gtcactggag gagcacgagt
    13261 gctccctggg acgccggggc gggttcatcg agcggctgcg cgacgggacc tggatgggcc
    13321 acgtgatgga gcacgtcgcg ctcgaaatcc agcggctcgc gggaattcat gtctcccgcg
    13381 ggaagacgcg tcgaaccggc gaacgtggca tttacaacgt cgtctacgcc taccgtcagg
    13441 aggacgttgg tctcgccgcg ggtcaacttt cggcgcgatt tctcaaccac cttatttatg
    13501 acgtagaacc tggtttcgat ttggtgcggg agttggagga acgagtcgtc cgcctcgccg
    13561 agcgtcttgc ctacggtcct tccacgctgg agatcgttca ggaagcagag cgccgcggta
    13621 tcccggtgat gcggctcaac tccaatcaat cgctcgtgca gcttgggcat ggccgctacc
    13681 agcgccgcat ctgggctacc cgcacctcca ccacctccgg cgtggcaatg accattgccg
    13741 gcaacaagga actgacgaac cgattgctcc gtgacgccgg cataccctct cccaggggag
    13801 ttgtggcggg gagtgcgcag gaagccgtcc gcgaagtgaa caggctcggc tacccggtcg
    13861 tgctgaagcc ggttgacggg aatcacgggc gcggcgtctg ccttaacctg agcaatcgca
    13921 ccgaagtaga ggagagcttc ccgttaccaa aggcggcgag ccgggagggt ttggtcctgg
    13981 tggagcccat ggtgccaggc aaggacttcc gcgtcgtggt cgtcggaagc gaggtagtag
    14041 ctgtcgcgga acgcgttccc gcctgcgttg ttggcgacgg gcaacactcg gtacgagagc
    14101 ttgttgacct gaccaatgtc gatccccgtc gcggtgtcgg acacgagaaa atactgactc
    14161 gtatccatgt cgattcccag acacacgagg ttctcagcgg tcagggactg acactggatg
    14221 acattcccgc agaccggcaa tgtgttcaac taaaactcac cgcgaatatg aacaccggcg
    14281 gcacatcgat cgaccgaacg gatgagattc atccggataa tgtcgaaatc gggctggaag
    14341 ccgcaatggt cgttggactc gacgtggcgg gcatcgactt cattacccct gatatctcgc
    14401 gatccgtgca cgaagcgggt ggcgcaatcg ttgagatcaa tgcggcaccg ggcttccgca
    14461 tgcataccca tcccacagaa ggtttgcctc gtcaggtagg ccacacggtg gtggatatgc
    14521 tcttccccaa cggcacacca gcctcgattc ccatcatcgc cgtcaccggc accaatggca
    14581 agacaaccac aacgcgtatg atcgcccaca ttatgcagaa ggatggccga accgttggca
    14641 tgaccaccag cgacggtatc tacgtgaacg gcacgcagat cgccgccggg gacaaggcgg
    14701 ggccagacag cgcccgcatg gtcctcagaa accctcgggt cgacatggcg gtgttggaga
    14761 ctgcgcgtgg cggtattctc cggtcgggac tcgggttcaa ccggtgcaat gtcgccgtgg
    14821 tcaccaatgt tgccagcgac catctgggga tgaagggagt ggacaccatc gaggacctgg
    14881 ccaaggtcaa gacggctctt cccgcttcgg tattgcgaga gggcacgagc gttctcaacg
    14941 ccgacaacca gtggacggtg gacatggcgc gcatcgcccg cggcgagatc atgtatttca
    15001 gcatggacga gaacaacgcg ttgacccgcg atcacgtccg aaagcagggt cgcgcggtcg
    15061 tgctcaggga aacaccgcgc ggcgggatgc tcacgctcgt tgagccaaag cgggagacaa
    15121 acatcctgct cgcccgcgat attccagcga ccttcaacga gcgggtgcgg ggtaacattg
    15181 ccaacgcgct ggccgtcacc gctgccgcga ttggcgctgg tgtaccgctc gcctgcattc
    15241 gggattcgtt gcgaaccttc agcaccgcat tttggcaaac gccaggccgc ttcaa
//
LOCUS       DASYWM010000100        38927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_341090, whole genome shotgun sequence.
ACCESSION   DASYWM010000100 DASYWM010000000
VERSION     DASYWM010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 38927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38927
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341090"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VGR29_03985"
     CDS             complement(<1..563)
                     /locus_tag="VGR29_03985"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEV2072782.1"
                     /translation="MSSSVFGRSWRWLAALLVLLIVGALGLLVMSTRPPDGDSPEAGM
                     LRDMLPHHTQAVEMALIIRDRTEDEQLRALATDIILSQQNQIGMMEGWLDLWDVSPNL
                     QGPMMSWMDHPVEAGELMPGMATAEQVESLRMSPVDEAEVAFLQLMIRHHQGAVHMAD
                     AYLERGDQEEVSEFARNIIAVQDREIET"
     gene            complement(626..1543)
                     /locus_tag="VGR29_03990"
     CDS             complement(626..1543)
                     /locus_tag="VGR29_03990"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEV2072783.1"
                     /translation="MSKQPRRKRQSQPGARKPSKQTQSVRATPRQRAAAPAPDLNEGT
                     SQASDASARREARRLQRQQQDQRTKMLRIGGAIAALVILGALAWGVYRWWMGPDVSGD
                     VTTYFGKDLAAAVHPENDTVILYEQVPPVGGPHNALWQTCGFYSEPIYNWHGVHAMEH
                     GAVWLTYDPSLPEDDIETLANKADQGYVLVSPYPGLDAPVVGSVWGKQIKFTGADDER
                     IDAFIKQYRLNPDNTPEPGATCSGGATLTSDQEPQQEPYQQVNPEATPIGGLTSIDAT
                     ATAAAEQNPVATPAATPAGRSEEDATPAT"
     gene            complement(1663..3084)
                     /locus_tag="VGR29_03995"
     CDS             complement(1663..3084)
                     /locus_tag="VGR29_03995"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEV2072784.1"
                     /translation="MSEQPENRRAATMEKIVALSKRRGFVFPGSDIYGGLANTWDYGP
                     LGVELRNNIKQLWWKTFVQCRRDMVGLDAGILMHPHVWEASGHVVNFNDPLVDCKTCK
                     ARFRADTLLEDKLGMEDAATRTPEEMTAIIKDANLACPNCGNRTLTDARQFNMMFSTT
                     IGPVTETGTKVYLRPETAQGIFVQYKNILATSRQKVPFGVGQIGKAFRNEITPGNFIF
                     RVLELEQMEIEYFVTPEDAPAQFETWLASMQEWVDLIGLKREHVRVREHAAEELSHYS
                     DRTVDYEYLFPGSMGWKELYGLANRTDFDLRQHQEHSGVDLSYFDQAANARYLPYVIE
                     PTFGVDRTFLTVLLDAYDEEETTDVNGKPETRVVLRISPRLAPFKAAVLPLAKKPELT
                     EVARDLFDQLQDATGVLIDYDETSNIGKRYRRQDEIGTPFCFTVDFDSLEDGAVTVRD
                     RDSMAQERIAVSEASVYLAGQIR"
     gene            complement(3217..4095)
                     /locus_tag="VGR29_04000"
     CDS             complement(3217..4095)
                     /locus_tag="VGR29_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV2072785.1"
                     /translation="MQDEIEHFLSSLQSDRQFSANTITAYRNDLNQLAGFLQRPPEDD
                     TSNRPLTAWSELTQDSLNTYLLFLGERSYASSTVARKTAAIKSFCVWLNTRGIVNGGI
                     GEHITAPRVDKYVPRAINPEDVDRLLAAPSRLAGAKPEAMRDRAMMEVLYATGMRVSE
                     LVSLNVDDVNLETGTVLCPGKAGRTRRLPLSPRALEAMCRYLSDGRRMLTGQNQNSLF
                     VNHRGGRLTRQGFWLILKAHAEKAGIKQMTPHTLRHSFAIHALRRGADIREVQRQLGH
                     VSLSTTQIYRDLAKQA"
     gene            complement(4227..5357)
                     /gene="ald"
                     /locus_tag="VGR29_04005"
     CDS             complement(4227..5357)
                     /gene="ald"
                     /locus_tag="VGR29_04005"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV2072786.1"
                     /translation="MIIGVPKEVKDRENRVAATPSAVHEYVSQGHKVLVQTSAGIGSG
                     YTDDEYVNEGAELIEDASGVWGRAEMILKVKEPIAQEYDLMRHGQILFTYLHLAADEP
                     LTRALIERNVQAIAYETVQLPNGQLPLLTPMSEVAGRMSIQVGAHFLERTRGGRGVLL
                     GGIPGVPPAEVVVIGGGVVGTNAAKIALGMGANVTVIDLNVDRLRYLDDVLDGRINTL
                     ASSRKNIADAVKTADLVIGSVLIPGRRAPKLVTTDMVSTMKRGSVTVDVAIDQGGCFE
                     TSRPTTHSDPAFEVDGVVHYCVTNMPGALPRTGTQGLTNVTTPYGIALANKGWERALR
                     DDPALALGANVINGHVTYAGVADAFNLPHVFLENAITGKPPA"
     gene            5825..7411
                     /gene="rseP"
                     /locus_tag="VGR29_04010"
     CDS             5825..7411
                     /gene="rseP"
                     /locus_tag="VGR29_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV2072787.1"
                     /translation="MGLLIVPILAFLIIIHELGHFFAARSVGVVVEEFGIGIPPRVKG
                     WKRNGVLWSLNAIPFGGFVRVKGEDGTDVSAGSMNSVGPLKRGWFLVAGPLMNLLAAV
                     VLSILIVGIQGKPVELAPLYIGTVAPASPAEEAGWRPGDKIVAVNGVKLDETQDVINA
                     INANAGEETDVVVERGTKLIETSLVPRTDPPPGEGATGIGVDNGRLSKVTIDEVQAGS
                     IAEEAGLLPGDEIVSINGIEIEAFAQAVGILNGSQGSEATITVIRDDSQRDITFQVPE
                     TSILVQNVSNDSPAAEASLYPGDRITEVDGTPVTSGSSFVKAIRDAAGESAELRYERE
                     GELRTTVIEVPPVPESTTNYHPVVSTGLAAAPVPAFQEAGISIVGTVIYEDVPPSQVV
                     QEGWGQFWAIITGTVEMIRSMFTQGIDRDQLTGPIGMGQLTSELLAETPEPAWVILTQ
                     LTILISVSLGVLNLLPLPALDGGRLLFVLIEIARGGKRIAPEKEGLVHLAGMVLLLGL
                     MFFIAFGDVTRIMDGESILP"
     gene            7538..8716
                     /gene="ispG"
                     /locus_tag="VGR29_04015"
                     /gene_synonym="gcpE"
     CDS             7538..8716
                     /gene="ispG"
                     /locus_tag="VGR29_04015"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEV2072788.1"
                     /translation="MSLIAPRRETRELHVGDVGIGGHNPIRVQSMATADTRDPKATLA
                     QITDLANVGCEIVRIAVPDRKAAAALPDIVPYSPIPLIADIHFEHTLALKALDAGIHG
                     LRLNPGNIRKEEDVREVVTKAKERGVPIRIGVNFGSVAPMTPEFVDEMAQQNATQVQL
                     RAEWLVRSALGHIKILEDLDFYDTKVSLKAFEVPVLLEAYQRFARLDNDYPLHLGVTE
                     AGTPRAGSVRSAVGIGTLLAMGIGDTIRVSLTTDPKEEVFVGYEILKSLELRQKGATM
                     IACPTCGRVEVDLFKLANDVDKVLEEVTEPIRVAVMGCVVNGPGEARDADVGIAAGNG
                     KGVIFRKGKIVKNCLEENMLEELTAEIYAVIEDRKNGIYDEIEEQPNAYRPMISLTAV
                     "
     gene            8900..10027
                     /locus_tag="VGR29_04020"
     CDS             8900..10027
                     /locus_tag="VGR29_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072789.1"
                     /translation="MSFNSLSRRSLISAATAGSLATVLSSRHSSSAQQSAPAGCSVPT
                     QASPVSSMTAAIEPVEIPSFEVPEGAIQVNMGTLPIAIYAPIYVAYEKGYYAQYGLDV
                     KPSGINSGTDIAVLTATNELQVGLSGIGPAFWNGIDTGLPLKIIAPGHQEGDPVASPL
                     MVARQACNDGTITSVEDLKGKRVSVNAPGATEYWLDAALGTGGLSIEDVDLQYLAFPD
                     AVTALGSGALDAGIIGEPLATQAEQQGILVRLASRFPVQGIQVTAVYANAEWVANNPD
                     AAAGFVAGYLQACRDLMEAPNDPLNLTIINKYTDVPIPLIADSVKPVYQENGDINIGN
                     LVMIQEFFGGRGLLDFDGTIDPNTVVEQQVIDDALVLLDSE"
     gene            10020..10928
                     /locus_tag="VGR29_04025"
     CDS             10020..10928
                     /locus_tag="VGR29_04025"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2072790.1"
                     /translation="MNKRINTPTVMAYVTGTPQPGAHHSPRRQPTLLKSRTVDSGARV
                     VARHVGRRFTTTDGSVDACVDLDLTVEPGSFCVIVGPSGCGKSTFLRMIAGLDHPTTG
                     TLDVSRPDGRTPTNAMVFHGRTLFPWLTLQQNVAYGLKLSGMDRNTRLERAQELLGMV
                     GLVDFQRAWPHQVSEGMRQRVSIARALAVDPDLLLMDEPFGALDEQTRYLLQEELLRI
                     WERTGKTVIFVTHSIDEALILADKIVVMSTHPGTIRKVIDVPFPRPRFLTEVRSDPAF
                     ADLFTTAWSILREEVETGGAFTEGTA"
     gene            10925..11701
                     /locus_tag="VGR29_04030"
     CDS             10925..11701
                     /locus_tag="VGR29_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072791.1"
                     /translation="MRRRRRSTELTLTIAGPIILLFAWEALSRTETINPLFWPPPSSL
                     WETTETLFLEKDLLGDIGISLYRIIAGFVVGTVPGIALGLAMGLFWPIRVFLMPLASA
                     IYAVPKIAILPLVIIMFGIGELSKIMIVAISIFFLVALNTMSGVLAIDTQFRDVARNL
                     GASRWELFWTVALPGSLPAIFTGMRLSLGFALVVIVGTEFLAADKGIGFMIWQSYQTL
                     RIQQMFVGLVITGIMGWALTLLLDLIEQVVVPWRTAGEEA"
     gene            11781..12338
                     /locus_tag="VGR29_04035"
     CDS             11781..12338
                     /locus_tag="VGR29_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEV2072792.1"
                     /translation="MTSATPILVATLLALQEQSVSPGLTLLMLIASVMTHAGCNLTND
                     YFDSIRGLDSVQTHGAGGTVHAGHITSDDLQAITISFSLALLLALPVMISIGWIVPVI
                     ALFAAGVAFFYTAGRYPLAYNRMDEVGGFLALGVAMVCGNYYVHAGTLTGTAFALSCS
                     IGLYAAAILHAKTCEMLRSTANMAR"
     gene            12621..13460
                     /locus_tag="VGR29_04040"
     CDS             12621..13460
                     /locus_tag="VGR29_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV2072793.1"
                     /translation="MKRSHLKPMVLMSGCVMLVLGVACALYSRFIHPFRPRVNHVMIL
                     LPRAHRELDGLTIAFVTDTHIGPHFTVEDLEPSIEILRRVRPDIVLFGGDYISESPRF
                     LDYATEPLAKMAAAAKIGAWGILGNHDLSNVRERVMGMLAPTGITILTNEAVEVTTEG
                     GAFWLVGIDDVLLGKPDLDQAFSNVPADALRIALWHEGDNASRAEPYEPLLLLSGHSH
                     GGQVRLPFLGALAAPKMGQRYTSGRYEIGSMTLFVSNGIGMYRPPVRFNCPPEVVIFR
                     LVA"
     gene            13647..14756
                     /locus_tag="VGR29_04045"
     CDS             13647..14756
                     /locus_tag="VGR29_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2072794.1"
                     /translation="MGQQEQLRVGIIGCGAGVFHLEGYQSESRAKVVALAGLDTDRCE
                     SLAKKFDIPRIYREYQDLLAQDDIDAVSVAVPNILHLPVALAAFEAGKHVLIEKPLAR
                     NAEEGVKMVEAAKTHGKLLGISFQRRTRHDVQLLRQQFEAGTFGRVYYSKAWWMRRSG
                     IPGLGTWFTSKEAAGGGPLIDLGVHVLDMILYVLGNPKVVSVSASTYAEIGTQGKGGW
                     AGQKRQKLKENQEYEVEDLATAFIRFEDGGTLLLEASWAAFTEMGDDFGIQIYGSKGG
                     ARIFSKDYALTDTLDVYTGFGDTTMDSKPRLVATGGHAEIIRGFVDAVLEGRPLSPEG
                     HEGLDRVRLIDAIYRSAELGREILIDEEIVDAAAD"
     gene            14770..15537
                     /locus_tag="VGR29_04050"
     CDS             14770..15537
                     /locus_tag="VGR29_04050"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEV2072795.1"
                     /translation="MTNPIRVTVWNEFRHEKKDEEVAKNYPDGIHGAIAEGLNQVDGI
                     EAKTATLDEPEHGLTVDIVANTDVFVWWGHMAHREVSDEIVDRVHQRVLEGAGLIVLH
                     SGHFSRIFKKLMGSSCDLKWREADEKERLWVVSPGHPIVAGIGEYIELEKEEMYGEHF
                     DIPQPDELVFVSWFQGGEVFRSGAAYQRGAGKIFYFRPGHETYPTYYNEEIRRVIGNA
                     VKWAAPVDRPARNFGNMDPLEDLPWYDRKHAVTEGFR"
     gene            15872..16774
                     /locus_tag="VGR29_04055"
     CDS             15872..16774
                     /locus_tag="VGR29_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072796.1"
                     /translation="MITSRGVQQHQARDHSHARRIPPHFLIGIVLVAIAWPLAWAGPA
                     PYSRYTFFPLWLGYILVADGLTVHRSGTSLLVRDRLRFALLFAFSIPLWWLFEYANEF
                     LQNWHYRGASRYSPVAYAALASLAFSTVMPAMFVTAELYRTFGYFSRERRWIRIAPSR
                     GGLLVIGAFGLALFVGSLTFPNVLFPFVWIGLFLLLDVINALTGGKSLAEQVARGQWD
                     TVLVLFAAGLTCGFFWEMWNFWSLPKWIYEVPFVSEPKLFEMPLLGYGGYLPFALEVY
                     AAYHLMHTLLFRRRDTYLRFDEPH"
     gene            17069..18199
                     /gene="solA"
                     /locus_tag="VGR29_04060"
     CDS             17069..18199
                     /gene="solA"
                     /locus_tag="VGR29_04060"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEV2072797.1"
                     /translation="MSERRADVIVVGGGTMGTAAGWALARRGHSVIVLEQFQHIHNLG
                     SHSGHTRIFRHAYFEGPLYVPWTVEADAAWVALQERTGIDLMVRCGCLDLAAPGSPHA
                     QQAAQSARAYDLPHEILSGDEVSSRFPAWTLPPNWEACFDPEAGILLIEPTLRALLME
                     FRAAGGVLHENQRVLAWESTSTGVTVTTASGAYEADRLIVTAGAWTGKLLADLNLPLE
                     VCRKPVMWFNTTDSDKFSSQSFPAFIAENGVEHYYGLPAYGDDSLKIGIHSGRNLTDP
                     DTINRNVEPEDLTADMRSFIDSCLDGAKPELTATSMCMYTMTPDEDFILDRHPMQDRV
                     ALAAGFSGHGYKFAPVIGEHLADLATKPASLPRGEFAISRFG"
     gene            complement(18446..18787)
                     /locus_tag="VGR29_04065"
     CDS             complement(18446..18787)
                     /locus_tag="VGR29_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072798.1"
                     /translation="MQIDPEPLYHHGLADRVIAYLQTKDTRLFPFTARFDEERQKAFI
                     HDLRDGLSDLTESGAKRGTSATGFLTSDKRLREVVASWAAAGGGWPETQDPTNPFGIS
                     GVAPFDQDPVP"
     gene            18952..21615
                     /locus_tag="VGR29_04070"
     CDS             18952..21615
                     /locus_tag="VGR29_04070"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV2072799.1"
                     /translation="MPDPVSGRRIISLDNGLTLFLQEKHDAPVASFWVWYRVGSRNEM
                     PGQTGVSHWVEHMQFKGTSSIAKGSIFRDVSRIGGQLNAMTSMDWTAYYETIPAAELD
                     LSLRIESDRMSNSLFDPPEVDSERTVILSERQGAENRPTYHLYEELIGAAFQSHPYSH
                     SVIGYERDLRRMSREDLFNHYRRYYRPSNAFITAVGDFDADDLASRINASFGSIPNND
                     MVIPDIAPDVPQYGERRVTIRKPSAAAYMMMGYKLPDAKHPDMPAILVADALLSGGKA
                     MGLGGGGVMGRSSRLYRALVSKGLARSAGSSASLYRDSYLWDFTATALPGVEPAAIEA
                     AVDAEIERLKNEIAGEEEFVKARKQSRAQYIYSQETATSQAFWLGQMEIVDHAGRVDT
                     LADELAAVTPEDVQRVARTWLVPEQRTIGWQLPDDGIVSAGVDIPDAALVEPFARTVC
                     GFSGEEVSSHGFVRTVLPNGIIVLAQPRPGDQAIDGAIEIQAGHAATGDTTPGLASMT
                     GSMLNRGTSNRSFDEYNEEVDGLGAIIGVSTSRDSMEIGWHSLAEDLEHVLGIAADII
                     LNPTFPEDELEKVRKQALTGLKEQEQDTGAMAAKALRELMYPEGHPYRIPTSGEIASV
                     EAIGRQDLVDYHAKHLGPTATTIALVGGVADLEAAVASLEQALGSWTNLIPQPHEVAS
                     IEPPATTVQEAITVRGRSQADITIGYPTIPRDHPEYYALQVANLILGQLGLMGRLGAH
                     VRDEQGLAYYAYSSLTGGKANSVWGSRAGVDPGNIGRALEGIERELRTIRKEHVSDEE
                     LADAKSYLIGSLPLGLESLGGVTDLLLTLERHKLGLDYLDRYPEIISALTKDDLLGAA
                     ATHIDPDRLAIGIAGPESVQKVVSRKLIQSF"
     gene            21759..22403
                     /locus_tag="VGR29_04075"
     CDS             21759..22403
                     /locus_tag="VGR29_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEV2072800.1"
                     /translation="MITSRNTITLRSVGVGKPALLGQRRGEEVLSGIRKQRVTLPTVD
                     VTTTNIAGDGQADLRNHGGFDKAVYCYPSEHLSFWGGTIGYAGDGNFAPFGENLSISG
                     IDEETACIGDIWRWGSVTLQISQPRWPCFKLDMHSGVKALMNQLIKSGRCGWYLRVLE
                     EGVAPTSGEIEIAGRDPLGISVRQAFDARRDPSLDPQRYEEIMSHPGLAAAWKR"
     gene            22564..24219
                     /locus_tag="VGR29_04080"
     CDS             22564..24219
                     /locus_tag="VGR29_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2072801.1"
                     /translation="MAPIPLPLRSLGQRMLTLLAGLLAGAIAAIVMTLAMAASRYWLG
                     IMPPVEAVPDRIARMLDIDSFFSLFGKYGGYNGLKKFGIVSGLRGVFGVGLVVGLVYA
                     LIIESASSRRSRRWLFGASTPALIFLAVASLAAWIGLVVFLWPVLPANYRGLPYSWAR
                     LATIAALFIWIALFSGTLMLVYRAITLRIVPITNGQTADPVDRPVSRILPRRAFLAVA
                     GGAALTIPIYRLLRTMYNDATFPYDGTVYSGEDIEPVTPTDRFYTVTKNVVDPDVYRD
                     IWRLEIGGHVDNSLSLSFDDLKQFEQVDQETTLMCISNRIGAGLFSNANWKGVRLRDV
                     LESAGIRDGAHETVLTGADAYRDTFPIEKALEESTLLVYQINGEPLPRVHGYPVRAIV
                     PGMFGEKNVKWITRVDVATEDVQGFYEQQGWGPNFEPYTRSDIFDPRTVLQGGSFSFR
                     QQFPANQPVEIRGRAFAGDRGISSIDISTDGGETWTRAEIYYAGTRLTWSLWRYRWTP
                     PKSGEYVITTRAFDAAGEPQTQEVRGIVPQGASGYHKVTMTVL"
     gene            complement(24280..25725)
                     /gene="pyk"
                     /locus_tag="VGR29_04085"
     CDS             complement(24280..25725)
                     /gene="pyk"
                     /locus_tag="VGR29_04085"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV2072802.1"
                     /translation="MERRVKIVATLGPAVSEPARLQDLMEAGVDVVRVNAAHGSAEER
                     AELITKVREAAEAIGKRVPILFDLRGLKIRTGPLPEGEDSVAVARGSRIRLEAGPVET
                     VPGRIGINFPNLFEVMSEGSRVLIADGLIELLVESIKGDHAMAMVARGGQLLGKQGVT
                     LPGAPIKGGALTDADKEDVAFAVKHGVDFLGLSFLNDASDLHLARQIASQHGDIVPGL
                     VAKIERPQALMNINEIVEAADGVMVARGDLGVQLPPERVPRAQKKIIGVANRVGVPVI
                     TATQMLESMITQPVATRAETSDVANAVWDGTDAVMLSAETAVGKYPIEAVRTMDRIIR
                     EVEKEGTVRSEAAKQKFAAEDDPESQFADAIARAAFALSDQTPVQHLVVFTKTGAAAR
                     RIAKYRPFPPIIAVADNERVARRLNLVWGIETVVLEVERDQDATFRKAGQLIIDQRLA
                     EDGEYALIVGSLPMSHEAGRTNLVHFRKLGT"
     gene            complement(25915..28944)
                     /locus_tag="VGR29_04090"
     CDS             complement(25915..28944)
                     /locus_tag="VGR29_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV2072803.1"
                     /translation="MEKLFGIPMTNIMLVMLVLLGTCLLTVAFIAWRRPVIFKLGVRN
                     IPRRKAQTTLIIIGLMLATLIISAALGTGDTLNRSVNSAVLTTLGPLDEIVLAATDDE
                     GNASIGTILTETMPESNVETVRAAVGDSDKVDAVAGILLSQSPVLNLGDSYPYNLQSI
                     DDILRAATASNPTVFIAGLDQTTIEDIGGMQDLNGNPVDVSTLGDNAVYVGKETAEDL
                     GLTTGDTIAYISGNEPYFAEVRGVLPDTVLTGSINPGTASMLMDLGRLQEITGQEGRV
                     SGIGISNAGGTREGLEYTEEVTSLLRTGLAEEDLGVVTIKQDNVEQAELIANIFVTFF
                     VVFGLFSIGVGVLLIVLIFTMLAAERRAEMGMERAVGAQRRQLIQQFLAEGAGYTLLS
                     GLIGTGLGVLATWAIAQGFSGLAEGLFSIELYVTPRSLVIAYSLGVVITFLAVAVSSW
                     RVSRLNVVAAIRDIPDSYNAHRNRKQLFWGVVMLAAGVLLAFLGMNVDQQAPFTIGMT
                     LIPLGIAAILTFFGANSRLVLTIAGIYLLLFWLLPSDLFTPVFGEMSGNIEMFFVSGI
                     CIVAASTLVIVQNLDAILSVITRYGGALRSMLPSVRLAVAYPGANKGRAGMTIAMFSL
                     IVFSLVMVAAINNNFANAFLSKQANAGMEIRVDVPDENPLGDFRDTLEREGVDTTQFR
                     GVGKMVSMDNLEEVKDEGGEWLSFIVLGMDEGYLDSAELEFQAHAEGYETDEAIIEAL
                     RTEPNVVVVNPAYAEDAPANFGGPPTPNLAGVPTEGPFAPATVEVRVPVGEVREMKVI
                     GVLDSSLSMLFFSYVGPPTVNAVMGGSSSSATSYFIDLADGADASAFASNIERVLLPY
                     GAQGVDIEQEMEESQAQQQTFMYVLQGFMGLGMIVGIAAVGVIAYRAVVERRQQIGML
                     RALGFQSGSIAQAFVIESAIVVVLGTLSGTITGLILSWVLLTSDEFSQGVDIQFSAPW
                     GTIIVTLAVAIFAALLMSYLPARQAGRILPAEALRYE"
     gene            complement(28994..29785)
                     /locus_tag="VGR29_04095"
     CDS             complement(28994..29785)
                     /locus_tag="VGR29_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2072804.1"
                     /translation="MRLGKKKMSVPAEVGENTIISANNVVKSYDTGTNKVQALKGITF
                     EVQRGEMVGVMGPSGCGKTTLLNCLSGLDDVDSGTIFIEGENLAEMSDKKRTNYRARR
                     MGFIFQVYNLLPVLSAVENVELPLLVSNVKSAEARTKSLEALERVGLRDWSGHRPAEL
                     SGGQRQRVTIARSLVNNPAIIWADEPTGALDSRSAGDIMDLLQKLNSDENLTIVLVTH
                     DPGVGQRCHRIVQMRDGEVVGEEFPDRSTTQAMAVRGNEAFSVVS"
     gene            30091..30732
                     /locus_tag="VGR29_04100"
     CDS             30091..30732
                     /locus_tag="VGR29_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072805.1"
                     /translation="MGWLRDLIAFIQERPERFLELVLAHLRLASTALLIASLIYIPLG
                     ILFSRMGRTGANAIGLLSSIRVIPSLALVFLFYPWLGFGTLPALVALIVLAAPALAVN
                     THAGLANVDAFTLEAARGVGMNSAQVFLQVQLPLALPVIVAGLRLAAVELLASATLAS
                     FVGAKTLGQYILTGISLLDTTYLLAGGIPIVILVLLADLMFSGIERLVTPPGH"
     gene            30768..31769
                     /locus_tag="VGR29_04105"
     CDS             30768..31769
                     /locus_tag="VGR29_04105"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV2072806.1"
                     /translation="MITRRSLAKTTAAAPIVGAGFFAAFQPAAFAQNGEPVVVGSKDF
                     TEQLILGHMYLQLLEDAGIPAEERLNLGGTQIAHEALVNGEISLYPEYTGTALSEVLQ
                     IPLEEVQASAVSSMASPMAGAESATPAASSDLSQAVYELVKTEYEDQFGLTWLERSPA
                     NNTQALAVKRAFSEETGITTISQLAENASEYTISAPADFPEREDGLLGLVRVYGENFS
                     EMEVLPVTPGLKYQALLDDEAEVVLAFGTDGQISGYDLVVLEDDLGLWPPYNVAPVVR
                     SEVLEAYPEIAERFNAVTTTLTDDVLSSLNWMVDGDEKMEPADVAVQYLTENGFIEE"
     gene            31861..32862
                     /locus_tag="VGR29_04110"
     CDS             31861..32862
                     /locus_tag="VGR29_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2072807.1"
                     /translation="MAINQRSGTSGSSISIHALTKRYPGSSAAAVNAVDLDIAPGELI
                     VLLGPSGCGKTTLLKMINRIYEPTSGSIVIDGVEVQSLKASELRRHIGYVIQQTGLFP
                     HMRVAENVAVVPKLLKWDRDRTRERVSQLLELTGLPPEIYANRYPSQLSGGEQQRVGL
                     ARALAAEPPTMLMDEPFGALDAITRTRLQDELKRIHKQFSQTILFVTHDIEEAVRLAD
                     RIVVMREGRIVQVGSPLEIVREPADQFVSDLVGSQDVLRRLSLMPARTAMEPLNGTPP
                     DLAQSISGEVFLREALSMLMESGDPFIAVTGSDGTAIGTLSMTSIQRAAAPPDAPNR"
     gene            32867..33514
                     /locus_tag="VGR29_04115"
     CDS             32867..33514
                     /locus_tag="VGR29_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2072808.1"
                     /translation="MDLGYFFDNLDEILALSLEHLRISLTALMIALPVALVISVIGVW
                     FRPARQPLLWTVGALYTIPSLAMLAFLIPSQGLGLRPTLILLVTYAQVFLVRNIVTGL
                     TGVDPAVLEAARGVGMTPLQVLSRVWLPIALPVILAGVRIALVTIIGLAILGGWVTAG
                     GLGELLFSGINTNHPAKVLAGLIAIVSLALLTDLAIRLLESFTSVARARRAGNRG"
     gene            complement(33587..33829)
                     /locus_tag="VGR29_04120"
     CDS             complement(33587..33829)
                     /locus_tag="VGR29_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072809.1"
                     /translation="MGTFLYYIVVGIAAGWLAGVITRTDRSIWGDLILGLVGAFVAGI
                     VADEIFADANIIVSIVIAAIFAAILVWLKNLILNRR"
     gene            complement(33964..35040)
                     /locus_tag="VGR29_04125"
     CDS             complement(33964..35040)
                     /locus_tag="VGR29_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072810.1"
                     /translation="MDYVVFGTGYGATLMLLGWALRTFGPGARYQDSGDGSVMGADAI
                     LARISWRRFAAALGSVIITAGASLILVTVALILINPGDETGALIALICFALILLAVAV
                     WTWLYVGRYGTHGILPERREEPAVVPARGMERVEPTAAMAIDGREADGISGTQEPDDD
                     IYEEPGIGEDHDGFAAVTNDDISEYDELESRYAKFLLHHPDDVVTVQQVHEDASAEIL
                     EDRGPSVADVNGNEVPEEAEELAAYGDPPDIEAPDDRLTAVIPDFQPVSNDEPQTVDT
                     IHISLNEDRPMEDVQLDDSSPTPDVNDLESDEEPLPGDVTATDGESDESGRALDLQDT
                     EEGRAEALRRLHAWQPPDTEEKNS"
     gene            complement(35303..36679)
                     /locus_tag="VGR29_04130"
     CDS             complement(35303..36679)
                     /locus_tag="VGR29_04130"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV2072811.1"
                     /translation="MVEIHWKDDLKTKEGQHLEELLTFLRMPGISTDPERTGDLRDSA
                     EWVANRLRAAGVPEVEIAPSAGHPAVIARWRVADDQPTILIYGHFDVQPAEPLELWDS
                     APFDPVVADGTIVARGSSDMKGNLLTAIQGVEAAAVANGGQPPVNVSFIFEGEEEIGS
                     PHFRDILRQHKDFLDADAVISADGGQFTSDTPSLAVSLKGLGGVQINLTTANTDLHSG
                     SYGAWVPNAVQSMVQLAATFHDEDGRVQVAGFYDAVRELSEAEKEEIARLPIDEDEEK
                     QKLDIDSLWGEAGYTPRERQWVRPTLDLNGIWGGFQGDGVKTVTPREAHLKVTCRLVA
                     DQDPLEVVELIRAHCEAHRPPGAKVDVVPLPGSARPYSVNREDPVFSAVKDVLTELYG
                     KDPVIVRNGGTVPATGIFQDELGVETITMAWAAPDSKAHAPNEWYSVADFYRGREGYA
                     MLLERLRR"
     gene            37049..37681
                     /locus_tag="VGR29_04135"
     CDS             37049..37681
                     /locus_tag="VGR29_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2072812.1"
                     /translation="MDTVQIIDQNQEDERLVARAQQGDLDAFNRLVVRHERALYNVCL
                     RLLRDPSQAEDATQDAFIRAWGAIDSFRGGQVRPWLLRIATNRAYDLLRSKSRRPAES
                     LDSQPFEIEPEWTSQAPGAEQPETFATRSELSAHLEHALAVLPEDQRLAIILSDVQGY
                     AYEEIAEVMNVAVGTIKSRISRGRSRLRAVLQGSERDRELTESFGRLNSV"
     gene            37823..38308
                     /locus_tag="VGR29_04140"
     CDS             37823..38308
                     /locus_tag="VGR29_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072813.1"
                     /translation="MITYMHTTNHLSDELLNSWVDGVVTADERSIICTHLEHCQLCRD
                     ELESLRSVKRLLSDLPQVRLPRSFQLTPEQARDRDTGTESTAHASENENPAHLQGSLG
                     EVIDEGQADSGSEVAVTGKALAFPEVSPRVLAAIAFTLAVVLAGIRVVAHRAGRLRPA
                     P"
     gene            38356..>38927
                     /locus_tag="VGR29_04145"
     CDS             38356..>38927
                     /locus_tag="VGR29_04145"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEV2072814.1"
                     /translation="MQSGIKSRPSGALLFPFLMALIVATVALLPSVAAAQSTPVAEET
                     VRTITVNGTGVVSTTPDTASVMLGIRSTNESLQTAQGDVTRRLTGVTQTLIEAGIAEE
                     DITTAQYTITPIPEYDRDGNYKGIQQYEVSVGLAVTVHDLDLLGTLLDSTVDAGVNDV
                     WGISMYVDDTSAAASEARTAAMADARARAE"
BASE COUNT         7587 a        12049 c        11317 g         7974 t
ORIGIN      
        1 gtttcgatct cccgatcctg gaccgcgatg atgttacggg cgaactcgct cacctcttcc
       61 tgatcgcctc gctcaaggta ggcatccgcc atgtgtacgg cgccctggtg gtgacgaatc
      121 atgagttgaa gaaaggcaac ctccgcttcg tccaccggtg acatccgcaa tgattccacc
      181 tgctcggcgg ttgccatgcc tggcatcagt tcccccgcct caactgggtg atccatccac
      241 gacatcatgg ggccctggag gttgggagag acgtcccata gatcgagcca cccctccatc
      301 atgccgatct gattctgctg tgagaggatg atatccgtag cgagcgcgcg gagttgttca
      361 tcctccgtgc gatcgcgaat gatcagcgcc atctcgacgg cctgggtatg atgcggcagc
      421 atatcgcgca gcatccccgc ctctggtgaa tcgccatcgg gaggacgcgt gctcatcacc
      481 aggagcccaa gcgcccccac tatcaggagc accagcaggg ctgcaagcca tctccagctc
      541 cgcccgaaga cactgctgga caccggttcg tttctttgtc cggcagacaa ggtggccacg
      601 tgatatcctc cgctacccgc gatcctcagg ttgccggcgt tgcgtcttcc tcgctccgac
      661 ctgctggcgt cgccgccgga gtcgcaacag ggttctgctc cgccgctgcg gtcgccgtcg
      721 cgtcgattga tgtcaatccg ccaatgggag tcgcctcggg gttgacttgc tggtacggct
      781 cctgttgggg ctcctggtcg ctcgtcaagg tggcaccccc tgaacatgtc gcccccggct
      841 cgggagtgtt atcgggattg agccggtact gcttgatgaa cgcgtcgatg cgctcgtcgt
      901 ctgcgccggt gaacttgatc tgcttacccc agaccgatcc aacgaccggc gcatctagcc
      961 cgggatacgg gcttacgagg acataccctt gatccgcctt gttcgcgagc gtctcgatgt
     1021 catcctcggg cagcgacgga tcgtaggtca gccagaccgc tccgtgctcc atagcatgga
     1081 cgccatgcca gttgtagatt ggctcggagt agaaaccaca ggtctgccag agcgcgttgt
     1141 gcggtcctcc aacgggaggc acctgctcgt agagaatgac ggtgtcattc tcagggtgaa
     1201 ccgccgccgc gagatccttt ccaaaatagg tcgtcacgtc ccctgagaca tcggggccca
     1261 tccaccaccg gtatactccc catgcaagcg cgcccagaat cacaagggcg gcaatggcgc
     1321 cgccaatgcg cagcatcttc gtccgctgat cctgctgctg gcgctggagg cgccgtgcct
     1381 cgcgccgggc ggacgcgtca gaagcctggg aggtcccctc gttcagatcg ggtgcggggg
     1441 cagcagccct ctgccgtgga gtagctcgga ccgattgtgt ctgtttcgaa ggctttcggg
     1501 cacccggctg cgactgtctc ttccgtcttg gctgcttgct catgtgctgg ctaatccttg
     1561 cgacaatatc aatcaattca aacgttcagg cgtttcttca cctgaacacg caacaaagct
     1621 ggctgcattc tgtcatggac aaacgcacat tagcatgctc gcctaccgaa tttgccctgc
     1681 caaatatacg ctcgcttcgg agacggcgat gcgctcctgg gccatactgt cccgatctcg
     1741 cacagtaacg gcgccatcct caagcgaatc gaaatcaact gtgaagcaga agggcgtgcc
     1801 gatttcatcc tgccggcgat aacgcttccc gatgttggag gtttcgtcgt agtcgatcag
     1861 cacaccggtc gcatcttgca gctgatcaaa aagatcacgt gccacctcgg tgagttccgg
     1921 ctttttcgcc agcggcagga cggctgcctt gaatggtgcc agacgcggcg aaatgcgaag
     1981 gacaacccgg gtctcgggtt tgccattcac gtccgttgtc tcttcctcgt cgtacgcatc
     2041 cagaagcacg gtcaggaacg tccggtcaac tccaaacgtt ggttcgatga cgtatggcaa
     2101 gtaccgcgca ttggccgcct ggtcgaagta ggacaggtca acgccggagt gctcctgatg
     2161 ctgacgcaga tcgaaatcgg tgcgattcgc gagcccatag agctccttcc agcccatcga
     2221 cccaggaaag agatattcgt agtcgaccgt acgatcggag tagtgcgaga gctcctcggc
     2281 ggcgtgttcg cgtacgcgga cgtgctcgcg cttcaggcca atcagatcga cccactcctg
     2341 catcgacgcc agccaggtct caaattgagc cggcgcgtct tccggcgtca cgaagtactc
     2401 aatctccatc tgctctagct caagcacccg gaagataaag ttgcccggtg tgatctcatt
     2461 gcggaaggcc ttgccgatct gtccgacacc gaacggcacc ttttgccggg atgtcgccag
     2521 gatgttcttg tattggacga agatgccctg ggctgtttcc ggccgcaggt aaaccttcgt
     2581 gccagtctca gttaccgggc caatcgtcgt gctgaacatc atgttgaact gccgggcgtc
     2641 agtgagtgtc cggttcccgc aatttgggca ggcgagattc gcatccttga tgatcgccgt
     2701 catctcttcc ggggtcctgg tggccgcgtc ttccatgccc aacttgtcct cgagcagcgt
     2761 atcggcccga aaccgcgcct tacacgtctt gcaatcaaca aggggatcgt tgaagttcac
     2821 gacatggccg gaggcctccc atacgtgcgg gtgcatcagg atcccggcgt cgagaccaac
     2881 catatcccgc cggcactgca cgaatgtctt ccaccagagt tgcttgatat tgtttcgcag
     2941 ctcgacgcca agaggaccgt aatcccatgt gttcgcaagg ccgccataaa tatcggaccc
     3001 cggaaaaaca aagccgcggc gtttgctcag ggcgacgatc ttctccatgg tggcggcgcg
     3061 acgattctcc ggctgttcac tcatctacgt gctgcttcct tgcatagtga tccagtggca
     3121 ctttcgtgcc actggtcagc gtagtttcgc tatcactata ggatgtcggg cagcactcgt
     3181 gcggaggcca cgtccgcctc tcgatcgctt ggacgatcag gcctgcttgg caagatcccg
     3241 gtagatctgc gtggtagaga ggctaacgtg ccccagttgt ctctgcactt cccggatgtc
     3301 cgcgccacgg cgcagggcgt ggattgcgaa cgaatgccgc aacgtgtgcg gcgtcatctg
     3361 tttgattccg gccttctcgg cgtgggcctt gaggatgagc cagaagcctt gccgggtaag
     3421 gcgtccgcca cgatgattga caaagaggga gttctggttc tgacccgtca gcatccgccg
     3481 accatcactg aggtatcgac acatcgcctc aagcgccctc ggtgaaaggg ggagccgacg
     3541 cgtccttcct gccttgccgg gacagagtac ggtccccgtc tccaggttca catcgtcgac
     3601 attcagcgac acgagttcac tgacacgcat accggtggcg tacagcactt ccatcatcgc
     3661 ccggtcgcgc atcgcttccg gctttgcccc tgccaaccgc gagggcgccg ccaagagccg
     3721 atcgacatct tcaggattta tcgctctggg cacgtattta tcgactcgtg gagcggtgat
     3781 atgctctcca atgccgccat tgacgattcc tctggtatta agccagacgc agaatgactt
     3841 gattgcagcc gttttccgtg ccacggtgga agatgcgtag gatcgctcac caaggaataa
     3901 caggtaggtg ttgagactgt cctgtgtgag ctcactccaa gcggtaagcg gtctgttgct
     3961 cgtatcatcc tcgggtggtc gctgcagaaa gcccgctaac tgattcaaat cgttgcggta
     4021 ggcagtaatc gtgtttgcgg aaaactgccg atctgactga agcgacgata aaaaatgttc
     4081 tatttcgtcc tgcatcccta aaaccttctc tcacatggct tagctcttac gcccttgatg
     4141 taaaagggca agcgctgtcg tcaacaacac ttgcccttcg ttcacatgtc cctgtgattg
     4201 ggcgatgctg gctgacttgc tttaccttac gcaggtggct tgccagtgat tgcgttttcg
     4261 agaaaaacgt gcggcaggtt gaaggcgtcg gccaccccgg cgtaggtgac gtggccattg
     4321 attacattcg ccccgagcgc gagcgcgggg tcgtctcgca gtgcccgctc ccatcccttg
     4381 ttcgccaacg caataccata tggcgtggtg acgttcgtga ggccttgggt gcccgttcgg
     4441 ggcagtgctc ctggcatgtt cgtaacgcaa taatggacga ccccgtccac ttcgaatgcc
     4501 gggtcgctgt gcgtcgtcgg gcgtgacgtt tcgaagcatc cgccctggtc gatggcgaca
     4561 tcgaccgtga cgctgccccg cttcatcgtg gaaaccatat cggtagtgac aagtttcggt
     4621 gcgcggcggc cggggatcag cacgcttcca ataacaagat ccgccgtctt aactgcatcg
     4681 gcgatgttct tgcgggatga cgccagggtg ttgatgcgac catcaaggac atcgtcgaga
     4741 tagcgaagtc gatcgacgtt gagatcgatg acggtgacgt tggcacccat gcccagagcg
     4801 atcttggcgg cattagtgcc cacgacacct ccgccgatga cgacgacctc cgcagggggc
     4861 acacccggga tgccgcccag cagcacgcct cgtccgcccc gggtccgctc caggaaatgc
     4921 gcgcccacct gaatcgacat gcgccctgcc acctcgctca tgggcgtaag taacggcaac
     4981 tggccgttcg gcagctggac ggtctcatag gcgatcgcct ggacgtttcg ctcgatcagg
     5041 gctcgcgtca aaggctcatc ggcggcgaga tgcagatacg taaagagaat ctgcccgtga
     5101 cgcatcaggt catattcctg ggcaataggc tcctttacct tcaggatcat ctcggcacgg
     5161 ccccagacgc ctgaagcgtc ttcgatcagc tccgctcctt cgttgacgta ctcatcatcg
     5221 gtgtaaccgc tacctatccc cgcagaggtt tgcacgagaa ccttgtgacc ctgcgagaca
     5281 tactcgtgta cggcagaggg cgtcgcagcg acgcgattct cgcgatcctt tacttctttc
     5341 ggtacgccga taatcatgtg gttcctccgg gctgacttcg ctggccccag agctggacca
     5401 gtcaacgatc ccgaacaccg atgttcgggc gctgtgacag aggctggtca cgctcgccat
     5461 cctcgcaata ggggacatgc gtgatgccaa ccagcagtgt ggccgaggca cagagattct
     5521 gcattcaccc cgtgattcta gaccctatca tgcgcccaaa tggtatcgca ctcccttgat
     5581 caacgcgaca ggatggaggt atgtgcaacc tgcgctttat gagagcggaa ttcgggatct
     5641 tcgatccttg acgctgcccc gcgatctggc ggccaacggc gcaggaacca ggggaaccgc
     5701 cgggttctgg taaaatagct tagagtggcg gaaacacctt cccctgtttg ccgcgttgca
     5761 ctgccacaag taatcccatc ctgtccgcac ggcagttgat tcactatctg aggcacatat
     5821 cttcatgggt ttattgatcg tcccgatcct cgcgtttctc atcatcatcc atgaactcgg
     5881 acatttcttc gccgcccgaa gcgtaggcgt cgtcgtggag gaatttggta tcggcatccc
     5941 tcctcgtgtg aaaggttgga aacgaaacgg cgtcctttgg tcgctgaacg ccattccttt
     6001 cggtggtttc gttcgcgtga agggcgaaga cggtaccgac gtaagcgcag gctccatgaa
     6061 ctccgtcggg cccctgaagc gcggctggtt cctggtggcc ggtccactga tgaacctcct
     6121 tgcagccgtc gtgctttcca tcctcatcgt cggcattcag ggcaagccgg ttgagctggc
     6181 accgctctat atcggtacgg tggcaccggc ctcgcctgcg gaggaggctg gctggaggcc
     6241 aggtgacaag atcgtcgctg tcaacggtgt gaagttggac gaaacgcagg acgtcatcaa
     6301 cgcgatcaac gccaatgctg gcgaggaaac cgatgtcgtc gtcgagcgtg gcactaagct
     6361 catcgaaaca tcgctcgttc caaggactga tccgccgcct ggcgaaggag ccactgggat
     6421 cggtgtagac aatggccgcc tcagcaaggt aacgatcgac gaggtacagg cggggtcgat
     6481 cgcagaagaa gccgggctgc tcccgggtga cgagatcgtt tcaatcaacg ggattgagat
     6541 cgaagcgttt gcccaggcag tgggaatcct gaacggttcc caaggctcag aagcgacgat
     6601 caccgtgatc cgggacgaca gccagcggga catcaccttc caggtgccgg agacgagcat
     6661 cctcgtccag aatgtcagta atgacagccc cgccgcggag gcgtcgcttt atccgggcga
     6721 ccgcatcacg gaagttgatg gcacacctgt aacgagcggc tccagcttcg tcaaggcgat
     6781 ccgcgatgct gctggcgagt ccgctgagtt acgctatgag cgcgaaggcg agctacgcac
     6841 cacggtgatc gaggtaccgc cggttccgga gagcacaacc aactaccatc cggtcgtatc
     6901 tacgggactt gcggcagcac ccgttccggc attccaggaa gccggcatca gtattgttgg
     6961 cacggtcatc tatgaggatg tgccgccctc acaggtcgtc caggaaggct ggggccagtt
     7021 ctgggcgatc atcactggca cggtcgagat gatccgtagc atgttcaccc aggggatcga
     7081 ccgcgaccag ttgactggac ctatcggcat gggccagtta acgtcagagc ttctggctga
     7141 aacaccagaa ccagcgtggg tgatcctgac acagctcacg atcctgatct cggtttcgct
     7201 tggtgtcctg aacctgttac cgctgccggc actggacggg ggtcggctgc tctttgtgct
     7261 gatcgagatc gctcgggggg gcaagcgtat cgcccccgag aaggaaggtc tcgttcacct
     7321 cgccggcatg gtcctgctgc tcggactcat gttcttcatt gcgttcggcg atgtcacccg
     7381 gatcatggac ggagaatcca tcctgcccta gtgacccgcc gatgagaacg cgtgaagagt
     7441 tggcatcaca tcgtagaatt gaagtcaaga acacgctgat cgatgtcgat tagcgccacc
     7501 acatcccagg cattcctgag ggaggcacca cccgctcatg tcactcatcg ctccccgccg
     7561 cgagacccgc gaactccatg tcggagatgt cgggatcggc ggccataatc ccatccgtgt
     7621 gcagtcgatg gccacggccg acacccgcga ccccaaagcc acgctcgccc aaatcaccga
     7681 ccttgccaat gtgggttgtg agatcgtccg catcgcggta ccggatcgca aggccgcggc
     7741 cgcactgccg gatatcgttc cctactctcc aattccactc attgccgaca tccacttcga
     7801 gcacaccctc gcgctcaagg cgcttgatgc ggggattcac ggtctgcgcc tgaaccccgg
     7861 caatattcgc aaggaagagg acgtccgtga ggtcgttacc aaggcgaagg aacgcggcgt
     7921 gccgatccgg atcggagtta atttcggctc ggtcgcgccc atgacgccgg aattcgtcga
     7981 cgagatggcg cagcaaaatg cgacccaggt gcagctgcgt gccgaatggc tggtccgctc
     8041 cgccctgggg cacatcaaga tccttgagga tctggatttc tacgatacca aggtcagtct
     8101 caaggcgttc gaggttcctg tcctgctgga ggcgtaccag cgcttcgccc ggctcgataa
     8161 cgattacccg ctgcacctgg gcgtcaccga ggcaggcacc ccgcgtgcgg gctccgtacg
     8221 ctcggcggtc ggtattggca ctctcctcgc catgggcatc ggcgatacga tccgcgtctc
     8281 gctgacaacc gaccccaagg aggaggtctt cgtcggttat gagatcctga agagcctcga
     8341 gctccgccag aagggcgcga cgatgatcgc atgcccgacc tgcggccgcg tcgaagtcga
     8401 tctcttcaag cttgccaacg atgtcgacaa ggtgcttgaa gaggtgacgg aaccgatccg
     8461 cgttgctgtg atgggatgcg tcgtcaacgg ccccggcgag gcacgcgacg ccgatgtggg
     8521 catcgcggcg ggcaacggca agggtgtgat tttccggaag ggcaagatcg tcaagaattg
     8581 cctagaggag aacatgctcg aggagctcac cgccgaaatc tatgccgtga tcgaagatcg
     8641 caagaacggc atctatgatg aaatcgagga acaaccgaac gcgtacaggc ccatgatctc
     8701 cctaacggcg gtctaggtta ggcttcctct cctccagcaa ctctcaatct cgctccgaac
     8761 aatcccgtgc ggggcgtgtc atggcgcgtc gtgaccgaat ctgctctcac cagaatccgg
     8821 atatacttac aggtgacctg tgtggagcaa cagaccggcg caaggatgcg ccgctgctcc
     8881 caaggagtaa ccgttaccga tgtctttcaa cagtctctcc cgccgcagcc tcatcagcgc
     8941 cgcaaccgct ggttcactcg caaccgttct ctcctcccgg cattccagta gtgctcagca
     9001 aagtgctccg gccggttgca gcgttccaac ccaggccagc cctgtctcat ccatgacggc
     9061 agcgatcgaa ccggttgaga ttccctcgtt tgaagttcct gagggagcta tccaggtcaa
     9121 catgggaacg ctcccgatcg cgatctatgc gcccatctat gtcgcctacg aaaagggcta
     9181 ctacgcgcaa tacgggctgg acgtcaagcc aagcgggatc aatagcggca ccgacattgc
     9241 cgtcctgacg gccacaaacg agctgcaggt cggactcagc ggtatcgggc cagccttttg
     9301 gaatggaatc gacaccggac tcccactgaa gatcatcgcg cccggtcatc aggaaggaga
     9361 tcctgtcgcc agtcccctga tggttgctcg acaagcctgt aatgacggca cgatcacgag
     9421 cgtggaggat ctcaagggca agcgtgtttc cgtcaatgcc cctggagcca cggaatactg
     9481 gctcgacgcc gcactaggaa cgggtggcct gagcattgag gatgtggatc tccagtatct
     9541 tgccttcccc gatgctgtta ccgctctcgg ttcgggtgcg ctggatgccg gcatcatcgg
     9601 cgagccgctc gctacccagg ccgaacagca ggggatactt gtgcggctcg catcacgctt
     9661 cccggtgcag ggaattcagg tcactgccgt ctatgccaat gccgagtggg ttgcaaataa
     9721 cccagacgcc gcagccggtt ttgtcgcggg ttaccttcag gcctgccgtg acctgatgga
     9781 ggcgccgaac gatccactga acctgacgat catcaacaag tacaccgatg tcccgatccc
     9841 gctgattgct gactcggtga aaccggtcta tcaggaaaat ggtgacatca acatcggcaa
     9901 cctcgtcatg attcaggagt tctttggcgg tcgaggtcta ctggacttcg acggcacgat
     9961 cgatccaaac acggtcgtcg agcagcaggt tatcgacgac gccctcgtcc tacttgacag
    10021 tgaataagag gatcaacaca ccaacagtta tggcctacgt taccgggact ccccagccgg
    10081 gtgctcacca cagccctcga cgccagccga cgctcttgaa gagccgtact gttgactccg
    10141 gtgctcgcgt cgtcgcacgg cacgtgggtc gccggttcac cacaaccgac gggtccgtgg
    10201 acgcgtgtgt cgaccttgac ctcaccgtgg agccggggtc gttctgcgtc atcgtagggc
    10261 cctctggctg cgggaaatcg acgtttctgc ggatgatcgc cggccttgat catccgacga
    10321 ccggtacatt ggacgtctcg cggccagacg gcaggactcc cacgaatgcc atggtcttcc
    10381 atggcagaac gcttttcccc tggctcacat tgcagcagaa cgttgcctat ggcctcaagc
    10441 tctcgggcat ggaccggaac acacgtcttg agcgggcaca ggagctgctg ggcatggtgg
    10501 ggctggtgga cttccagcga gcgtggccgc accaggtatc agaaggcatg cgccagcggg
    10561 tatcgatcgc ccgcgcactc gccgtcgatc cagatctctt gctcatggat gaaccgtttg
    10621 gtgcgctgga cgagcaaact cgctacctgc tgcaggaaga gttgttgcgg atctgggaac
    10681 gcaccggcaa gacggtcatc ttcgtcacgc attcgatcga tgaagccctg atcctcgcgg
    10741 acaagatcgt tgtgatgagc acgcaccccg gaacgatccg gaaggtcatc gacgtaccat
    10801 tcccgcggcc ccgtttcctg accgaggtta gatccgaccc ggccttcgcc gacctcttta
    10861 ccaccgcctg gtccatcctt cgagaggaag tcgagaccgg aggagccttc acggaaggca
    10921 ccgcgtgaga cgccgccgcc gttccactga actcacgctg accatcgcgg gacccatcat
    10981 cctgctcttt gcatgggaag cactgtcccg gaccgagacc atcaatcccc ttttctggcc
    11041 accacccagt tccctctggg aaacgaccga aactctcttt ctcgagaagg atttgctggg
    11101 cgatatcggg atcagcctct atcgcatcat cgccgggttc gtggtcggga ccgttcccgg
    11161 aatcgcgctc gggttagcga tggggctctt ctggccgatc cgggtcttcc tgatgccact
    11221 ggcctcggcc atctacgcgg tccccaagat cgcgatcctg ccgctggtga ttatcatgtt
    11281 cggcatcggc gagctttcca agatcatgat tgtcgccatc tcgatcttct tcctcgttgc
    11341 cctcaacaca atgagcggtg tgctcgccat cgatacgcag ttccgcgatg tcgcccgcaa
    11401 cctcggtgcc tcacgatggg agctcttctg gaccgttgcg cttcccggca gccttcctgc
    11461 catctttact ggaatgcgcc tctcactcgg gtttgccctg gttgtgattg tcggtacgga
    11521 atttctcgcc gccgacaagg gtatcggatt catgatctgg cagagctacc agactctccg
    11581 catccagcag atgttcgttg ggctggtgat cacaggcatc atgggctggg cgctgacgct
    11641 gctgctcgac ttgatcgaac aggtggtggt gccttggaga accgccggcg aggaagcgtg
    11701 acgtgattgc gagccggagc aatgcaactt cgccccctcg ttggcgcgta tggctggtcg
    11761 cctctcgccc ccgaacacaa atgacctcag ccactcccat cctggtagca accttgcttg
    11821 cgttgcagga gcaatcggta tctccagggc tgacgctcct gatgctgatc gcgtccgtca
    11881 tgacgcacgc cggctgcaac ttgaccaatg actacttcga cagcatccgc gggctggaca
    11941 gtgtccagac acatggagcc ggtggtacgg tgcatgccgg tcacattacc agcgatgatc
    12001 ttcaagcgat cacgatatcg ttcagtcttg cactgttgtt agccctgccc gtgatgatct
    12061 caataggctg gatcgttccg gtgattgccc tcttcgctgc tggcgttgcc ttcttttaca
    12121 ctgcagggcg atacccactt gcctacaacc ggatggacga ggtcgggggc ttcctggcct
    12181 tgggagtggc aatggtctgc ggcaactact acgtccacgc cggcacgctg actggtacag
    12241 cttttgccct ttcgtgttcg ataggcctct atgccgccgc catccttcac gccaaaacat
    12301 gcgagatgct gaggtcgacc gcaaacatgg caagatgacg ctcgccgaca cgctgggaag
    12361 acctgggacg ttcacgaata cgctctactg gtccttatgc agttgctcat cacggtcgcg
    12421 ctggtgctcg tgcgacccca attctggccg ttgctcgtga cactgcttgt gctcccactc
    12481 ccgctgcaag tagcgagcct tctgcggcat tgtcagactg tcgccgaata tgacagcgtg
    12541 atgacacagt gcgcgcaact gcttatgagg tatgcggtgc tcgtcggtat cggtctcgtc
    12601 acttcgagga ttcctgaaca atgaagcgat cccatctcaa gccaatggtc ctgatgtccg
    12661 gctgcgtcat gcttgttctc ggagtggcat gcgcgcttta ctcccgcttc attcatccct
    12721 ttcggccacg agtcaatcac gtcatgatcc tgttgccacg tgcgcacagg gaactggatg
    12781 gtctcaccat cgcgttcgtc acagacacgc acatcggtcc gcatttcacc gtcgaggatc
    12841 tggaaccctc catcgagata ctgcgccgtg tcaggcccga tatcgtcctc tttggcgggg
    12901 attacatctc tgagtcccct cgttttctcg actatgcgac agagccactg gcgaagatgg
    12961 ccgcggccgc gaagatcggc gcgtggggca tcctcggcaa ccacgatctg tccaatgtgc
    13021 gggagcgtgt gatgggaatg cttgcaccta ccggcatcac gatactcacc aatgaggcgg
    13081 tcgaggtcac cacggaaggg ggtgcattct ggcttgtcgg gatcgatgac gtgctcctcg
    13141 gaaagcccga tctggatcag gcattctcga atgtgcctgc cgatgctctc aggattgctc
    13201 tctggcacga gggtgacaac gcttccaggg ctgagccgta cgaaccgctc ctgcttctga
    13261 gtgggcactc gcatggtgga caggtacggt tgcccttcct cggtgccttg gccgcgccga
    13321 agatggggca acgatacacg agcggacgct acgagatcgg ctcgatgact ctcttcgtct
    13381 cgaacggaat cgggatgtat cgtccaccgg tgcgcttcaa ctgtccgcct gaggtggtca
    13441 tcttccggct tgtggcctga ggattgctcc agcgcagccg ttgtgcattg ctcgctacgg
    13501 tgacatgctc acaccaacag gacggttcac cggcatctgg cacaagggtg aaccagaata
    13561 cggttgaaga cctgggcggg tctgccggaa gatgcggaaa gcatggtcac tcgctggttg
    13621 aacgatgtca cgcgtgagga acgtatatgg ggcaacagga gcaactccgg gtcggcatta
    13681 tcggctgcgg agccggggtc tttcatctcg aggggtatca gtccgagtca cgggccaagg
    13741 tcgtggcgct ggcgggcctg gacaccgacc gctgcgaatc gttggcaaag aaattcgaca
    13801 ttccacgcat ctaccgtgaa tatcaggatt tgctggcgca ggacgacatc gatgccgtca
    13861 gcgttgcggt gccgaatatc cttcatcttc cggtcgcgct ggccgccttc gaagccggca
    13921 aacacgttct catcgagaag ccgctggccc gcaacgccga agagggcgtg aagatggtcg
    13981 aagccgcaaa gacgcacggc aaacttctgg gcatctcctt tcagcggcgc acgagacacg
    14041 atgtccagtt gctccggcag cagttcgagg ccggcacctt tggccgggtc tactattcaa
    14101 aagcctggtg gatgcgccgg agtggcatcc ctggtctcgg cacctggttc acttccaagg
    14161 aagcagcggg tggaggccca ctgatcgacc tcggtgttca cgtgctcgat atgatcctct
    14221 acgtcctcgg caacccgaaa gtggtctcgg tttctgccag cacctacgcc gagatcggta
    14281 cccaggggaa gggcggctgg gcgggccaga agcgccagaa actcaaggag aaccaggaat
    14341 acgaggttga ggatctcgcc accgccttca tccggtttga ggatggtggg accctgctgc
    14401 tcgaggcctc ctgggcggcg ttcacggaga tgggtgacga cttcggcatc cagatctatg
    14461 gatcgaaagg cggagcgcgc atcttctcca aggactacgc actgactgat acgttggatg
    14521 tctacactgg cttcggcgac acgacgatgg acagcaagcc gcgcctggtt gccaccggtg
    14581 gccatgcaga gatcattcgt ggcttcgtag atgcggtcct tgaaggtaga ccgctgtcac
    14641 cggaggggca cgagggcctg gatcgcgtcc ggttgataga cgcgatctac cgctcggccg
    14701 aactgggtcg agagattctg atcgacgaag aaatcgtcga tgccgccgcc gactagaaag
    14761 gaaatgctca tgacgaaccc gattcgtgtg accgtctgga acgagttcag gcacgagaag
    14821 aaggatgagg aagttgcgaa gaactatccg gatggcattc acggagccat cgcggagggt
    14881 ctgaaccagg tcgacggtat cgaggcgaag acagcgaccc tcgacgagcc cgagcatggg
    14941 ttgaccgtgg atatcgtcgc aaatacggat gtcttcgtct ggtggggcca tatggcgcat
    15001 cgcgaggtca gtgacgagat tgtcgatcgc gtgcaccagc gcgtgttaga gggtgctggc
    15061 ctgatcgttc tccattccgg gcatttctcg aggatcttca agaagctcat gggctcgagc
    15121 tgcgacctca agtggcgtga ggccgacgag aaagagcgtc tctgggtcgt ctctcccggt
    15181 catccgatcg ttgccggtat cggggagtac atcgagctcg aaaaagagga gatgtacggc
    15241 gagcacttcg acattccgca gcccgatgag ctcgtcttcg tcagctggtt ccagggtggc
    15301 gaggtcttcc gaagcggcgc ggcatatcag cgcggtgccg gcaagatctt ctacttccgg
    15361 cccggccatg agacttatcc gacctactac aacgaggaga ttcgtcgggt gatcgggaac
    15421 gccgtgaagt gggccgcgcc tgtcgacagg cctgcgcgca atttcggtaa tatggacccg
    15481 ctggaggatc tcccctggta cgatcgcaag catgccgtca ccgagggctt ccggtaggca
    15541 tctcacacga cactgatgac agcactgccc gagcttcatg cccgggcagt gctgtacctc
    15601 ttccggattt cgcgaatcgt aacggaggct tgctacgtcg tccggagtcc gcccagctaa
    15661 ctcaggggat gcaccagagc tatcgcggca tgaagctcgc gatgagggag gcacagagat
    15721 ggggcaggat catcccctaa agcgcccgcg taaagccgga caggttccat gaacgtgcgg
    15781 ggaatagcaa tctcgcccat cgtcgaggtc tttttacctt gtgggacaag atgggcagcg
    15841 taatgatgtg caaggataaa gcgggagaag catgatcact tcacgaggag ttcaacagca
    15901 tcaggctcgg gatcatagcc acgcacgtcg cattccgccc cacttcctga tcggtattgt
    15961 gctggtcgca atcgcctggc ccctcgcgtg ggccgggcct gccccctact cccgctacac
    16021 gttctttccg ctttggctgg ggtacatcct cgtcgccgat ggcttgacgg tgcatcgctc
    16081 cgggacatcg ctccttgtgc gtgaccggct tcgcttcgcg ctcctcttcg ccttttcgat
    16141 tccgctctgg tggctcttcg agtacgccaa cgagttcctc cagaactggc actaccgcgg
    16201 agcaagccga tacagccccg tcgcctatgc cgctctcgca tccctcgcgt tctctacggt
    16261 gatgcccgcg atgttcgtaa cggcggaact ctatcgcacg ttcggatact tctcgcggga
    16321 gcgccgttgg atccgaatcg ctccttcgcg tggggggctg ctcgtgatcg gcgcatttgg
    16381 tctggcgctg ttcgtcggat cgctcacgtt cccgaatgtc ctcttcccct ttgtctggat
    16441 cgggctgttc ctgctgcttg atgtgatcaa tgccctgacg ggcggcaaga gcctcgccga
    16501 gcaggttgcc cggggccagt gggacacagt gcttgtcctc ttcgccgccg ggcttacctg
    16561 cggtttcttt tgggagatgt ggaatttttg gtccctgccg aagtggatct atgaagtgcc
    16621 tttcgtttcc gaaccgaagc tcttcgagat gcccttgctc ggctatggcg gctatctccc
    16681 gttcgcattg gaggtgtacg cggcctacca tctcatgcat acactgctgt tccgccgacg
    16741 cgatacctac ctccgcttcg acgagccgca ttagccagca accatgcagg ggagaaacca
    16801 ggaacgaccg gagcagggcc aagccgtccc ttgttcatag acgggggaca ggttcacctg
    16861 agtctccttg caagcaagac ggctcggccc ctcgaggata ccctccacgg cccggtcgga
    16921 cgacctctgg cccctgtctt cgccgccgac ctgacttgcg ttcgggtcca cgaccgcgat
    16981 acttcaggaa ttggctaacg aggcattcgg gacaagggca cttcccggcg gcaccatgaa
    17041 aaacctgggt agttatgcag gagcggcaat gagcgaacgt cgagctgatg tcattgtggt
    17101 cggtggcggc accatgggaa ccgccgcggg atgggcactt gcccggcggg gccactccgt
    17161 aatcgtgctg gagcaattcc agcatatcca caatctcggc agccacagcg ggcacacccg
    17221 gatcttcagg catgcctact tcgagggtcc tctctatgtc ccatggaccg tcgaggcaga
    17281 tgctgcctgg gtggcgctgc aggagcggac agggatcgat ctgatggtcc ggtgcggatg
    17341 tctggatctt gccgctcccg ggagccctca tgcacagcag gcggcccaga gcgcgcgagc
    17401 ctacgatctg ccgcacgaga tcctgtccgg tgacgaggtc agctctcggt tcccggcatg
    17461 gacactccct cctaactggg aggcgtgttt tgacccggaa gcgggcatcc tgctgatcga
    17521 gccgacgctt cgagcccttc tcatggagtt ccgggcagcc ggaggggtgc ttcatgaaaa
    17581 ccagcgagtg cttgcctggg aatccacatc gacaggcgtg acggtaacta cagcgagcgg
    17641 ggcgtatgaa gccgaccggc tcatcgtcac cgccggtgca tggaccggca aactgcttgc
    17701 cgatctgaat ctgccgctgg aggtttgccg caagccggtg atgtggttca acactaccga
    17761 ctcggacaag ttcagtagcc agagcttccc ggcgttcatt gccgaaaatg gcgtcgagca
    17821 ctactacggg ctgccagcgt atggcgatga cagtctaaag ataggcattc acagtggccg
    17881 caatctcacc gatcccgaca ccattaaccg caacgttgag ccagaggatc tcacagccga
    17941 catgcggtcc ttcatcgatt cctgcctgga cggggcaaag ccggaattga cagccacgag
    18001 tatgtgcatg tatacgatga cgccagacga ggatttcatc ctcgaccggc acccgatgca
    18061 ggatcgcgtc gccctcgccg ccgggttcag tggccacggc tacaagttcg ctcccgttat
    18121 cggggagcac ctcgccgacc tggcgacgaa gccggcgtcc ctgccccgtg gcgaattcgc
    18181 aatcagcagg tttggctagt ggtgtagctc ccgcccccga gcagaagctc tcgacaagaa
    18241 ggaccaactc ccggaacgag acacctatca ctgccacacc caaggtcacg ctcggcaatg
    18301 tcgaggtcgc ggcggcggcg taacgctcat cctgacacct cttccgccgg gtgaaaggga
    18361 gcacgaaatc tgtaaacaca aaacgacgcc cccggacgat ggtccgggaa cgtcgttgtt
    18421 cttgttcagc agtaggcgga cgaatctacg gaaccgggtc ctggtcgaac ggcgcgacac
    18481 ccgaaatccc gaacgggttg gtcgggtcct gcgtctccgg ccatccaccg cccgccgccg
    18541 cccagctggc aacaacctcg cgcaggcgct tgtcgctggt gaggaacccg gtcgcggagg
    18601 tgccgcgctt ggcgccgctc tccgtaaggt cgcttaaacc gtcacgaagg tcatggatga
    18661 aggccttctg acgctcctca tcgaagcggg cggtaaacgg gaacaaccgc gtgtccttcg
    18721 tctgcaagta ggcaatcacc cggtcggcga gtccatggtg atacagcggc tcaggatcga
    18781 tctgcacagg aacactcctt ctcaaagccg ccaatcgcgg gaaagacgca cgttcttgcc
    18841 cgattgtatc tggcatgccc gactactggc gagtccctgc caagcctgcc cgtatacttg
    18901 cccaagcatt ggcttcccgg cgcctagcgc tcattaaagg accctcatta aatgcccgat
    18961 cccgtttccg gccgtcgcat catctctctc gataacggcc ttacgctctt cctccaggag
    19021 aaacatgacg ctccggtggc ctcgttctgg gtctggtacc gggtcggaag tcgtaatgaa
    19081 atgccaggac agacgggggt ctcgcactgg gttgagcata tgcagttcaa ggggacgtcc
    19141 tcgattgcca aaggctccat ctttcgtgac gtttcgcgca tcggtggtca gctcaacgcg
    19201 atgacgtcca tggactggac ggcatattac gagacgatcc cggcggcgga gcttgacctc
    19261 tcgctgcgca tcgaatctga ccgcatgtcg aattcgctct ttgatccacc cgaggtcgac
    19321 tccgagcgga ccgttatcct ctccgagcgc caaggcgcgg aaaatcgccc tacctatcat
    19381 ctctatgaag aactcatcgg cgccgcgttt cagtcccacc cctactcgca ttccgtgatt
    19441 ggctacgaac gggatctccg gcgcatgtcg cgggaggacc tcttcaacca ctaccgccgc
    19501 tactatcgtc ccagcaatgc atttatcacc gccgtgggcg acttcgatgc cgacgacctc
    19561 gcctcccgca ttaatgcatc gttcggcagt atcccgaaca acgatatggt gatcccggac
    19621 atcgctcctg atgtcccgca gtacggggag cgtcgggtga ccatccgtaa accctctgcg
    19681 gctgcctaca tgatgatggg ctacaaactt ccagacgcga agcatccgga catgcccgca
    19741 attctcgtgg cagatgccct gctctccggg ggcaaggcaa tgggactcgg gggcggcggc
    19801 gtgatgggcc gctcctcccg cctctatcgc gcactcgttt ccaagggcct cgcccgttcc
    19861 gccggatcat cggcgtcgct ctatcgcgat tcctatctct gggacttcac cgcgacggca
    19921 ttgccgggcg tcgaaccagc ggcgatcgag gctgctgtcg acgcggagat cgagcgcctg
    19981 aagaacgaga tcgcaggcga ggaagagttc gtcaaggccc gcaagcagag ccgcgcccag
    20041 tacatctact cacaggagac cgcgacgagc caggcgttct ggctgggcca gatggagatc
    20101 gtcgaccacg ctggccgggt cgacaccctc gctgacgagt tggcagctgt cacccctgag
    20161 gatgtccagc gtgtggcccg gacatggctc gtgcccgagc agcgaactat tggatggcag
    20221 ctcccggacg acgggattgt aagcgccggt gtcgacatcc cggacgccgc tcttgttgaa
    20281 cctttcgcgc gaactgtgtg tgggttttcg ggcgaagagg tgtcgtcgca cggatttgtt
    20341 cggaccgtgc tacccaacgg catcatcgtg cttgcgcagc cgcgcccggg tgaccaggcg
    20401 atcgatggcg cgatcgagat acaggcagga catgccgcga cgggtgatac cacccccggt
    20461 cttgcctcga tgaccggttc gatgctgaat cggggcacct cgaaccgatc cttcgatgag
    20521 tacaacgagg aggtcgacgg tctcggcgca attatcggcg tgtcgacctc ccgcgactcc
    20581 atggaaatcg gatggcactc gcttgcggag gatttggaac atgttcttgg gattgccgcc
    20641 gacatcatcc tcaaccccac ctttcctgag gacgagctgg agaaggtacg caagcaggcg
    20701 ctgactggcc tcaaggagca ggaacaagac accggggcga tggctgccaa ggcgctccgt
    20761 gagctgatgt atccggaggg tcatccctac cggattccga ccagtggcga gattgcctcc
    20821 gtcgaggcaa ttggccggca ggacctggtg gactaccatg ccaagcacct cggaccgacc
    20881 gcgaccacca tcgcattggt cggcggcgtg gccgatctgg aggccgccgt ggcgagcctg
    20941 gagcaggccc tcgggtcctg gacgaacctg ataccccagc cgcacgaggt tgcatccatt
    21001 gaaccgccgg ccacaactgt ccaggaggcg atcacggtcc gtggccgtag ccaggcggat
    21061 atcactatcg ggtacccgac gatcccacgc gaccaccctg agtattacgc cctccaggtc
    21121 gcgaacctga tccttggcca gcttgggctc atgggccggc tcggtgcaca cgtgcgggac
    21181 gagcaaggac ttgcctacta cgcctacagt agcctcaccg gaggcaaggc aaacagcgtg
    21241 tgggggtcgc gcgccggcgt tgacccgggc aatattggcc gggcactgga gggcatcgag
    21301 cgggagctga ggacgatccg caaggaacac gtctcggacg aggagctggc cgacgcaaaa
    21361 agctatctca tcggctcctt gccgctgggg ctggaatcgc tcggtggtgt gaccgacctg
    21421 ctccttaccc tggagcggca caagctgggt ctggattacc tcgacagata tccggaaatc
    21481 atctcagcgc tcacgaagga cgatctgctc ggggctgctg cgacccacat tgacccggat
    21541 cgcctcgcga tcggtatcgc cgggcccgaa tcagtccaga aggttgtctc acgtaagctg
    21601 atccaatcct tttgatccgg ggcctgtcat ccccagccga tgagatcgcg cagcggtcga
    21661 atcgtgtcga acgatctgga gaccggaaat cccgggttgg gagatccgtc gacgtcgctc
    21721 gggatgacga ggattgacgc gaaactgttg aggccacaat gatcacatcc agaaacacca
    21781 tcaccctgcg ctccgtcggc gttggcaagc cggcgctgct tgggcaacgc cgtggcgagg
    21841 aggtcctctc cggcatccgc aagcagcgcg tcactctccc cacggtcgat gtcacgacaa
    21901 cgaatatcgc cggcgatggc caagccgacc tgcggaacca tggtgggttc gacaaggcgg
    21961 tctactgcta cccgtccgag catctctcct tctggggtgg gaccattggc tacgctggcg
    22021 acggcaactt tgcgccgttc ggggaaaacc tgtccatctc gggcatcgac gaggagacgg
    22081 cgtgcatcgg cgatatctgg cggtgggggt cggtgacact acagatcagc caaccccgtt
    22141 ggccctgttt caagctggat atgcactctg gcgtcaaagc actcatgaac cagctgatca
    22201 agtccggtcg ctgtggttgg tatctgagag tactcgagga aggtgtcgcc ccgacctccg
    22261 gtgaaatcga gatcgccggg cgggacccgc tgggcatcag cgtccggcag gcgttcgacg
    22321 cccgccgcga tccatctctc gacccgcaac gctacgagga aattatgtcg catcctgggc
    22381 tcgccgctgc ctggaagcgc tgaacctctc gctgtaatcg ggcactcccg ggacgatcac
    22441 caccgcgtca aaaggcgcat ctgcccgggg ggacaatcca gaagccggtt tcctccgtat
    22501 actcgactgt gctccctgca gggtcggcac aagtttctgt gcctgaccaa agggatatgt
    22561 cgtatggccc caattccgct tccactccgc tctctcggcc aaagaatgct gacgctgctt
    22621 gcgggtctcc tcgcaggcgc catcgccgca atcgtgatga ccctcgccat ggccgcaagc
    22681 cgctactggc tgggcatcat gccgccagtc gaggccgttc ccgaccgcat agcccggatg
    22741 ctcgacatcg attcattctt cagtctgttc ggaaagtacg gcggctacaa cgggctcaag
    22801 aaatttggta ttgttagcgg tcttcgcgga gtctttggcg tcggtttggt ggtgggtctt
    22861 gtctacgcgc tgatcatcga aagtgcatcc tctcgacgga gtcggcgctg gctcttcggg
    22921 gccagcacgc cagcgctcat cttcctggca gttgcctcgc tcgctgcttg gatcggcctc
    22981 gtggtctttc tctggccagt ccttcccgcc aactatcgtg gtctccccta ctcctgggcg
    23041 cgtctcgcca cgatcgcagc tctctttatc tggatagcgc tcttctcagg cacgttgatg
    23101 ctggtctacc gcgcgatcac gttacggatc gttcctatca ccaacgggca gacggcggat
    23161 ccggtggatc ggcccgtctc ccgcatcctt ccccgccgcg ccttcttagc ggtagccggt
    23221 ggtgcagccc ttaccatccc gatctaccgc ctgttacgga cgatgtacaa cgacgccacc
    23281 tttccctacg acggcaccgt ctatagcgga gaagatatcg agcctgtcac gccgacggac
    23341 cgcttctaca ccgttaccaa gaacgtcgtc gaccctgatg tctaccgtga tatctggcgt
    23401 cttgagatag gcggtcatgt cgacaactct ctctcgctct cgttcgacga cctgaagcag
    23461 ttcgagcagg tagatcagga aacgaccctg atgtgcatca gcaaccggat cggcgccggg
    23521 ctcttcagca atgcgaactg gaagggcgtg cgactgcggg atgtcctgga atctgccgga
    23581 atcagggacg gcgcccacga gacggtcctc accggcgcgg acgcctaccg ggataccttc
    23641 ccgatcgaga aagcccttga ggagagcacg ctgctcgtct accagataaa tggtgagccg
    23701 ctgccacggg tgcatgggta tcccgtgcgc gcaatcgtgc ccggcatgtt cggtgaaaag
    23761 aacgtgaagt ggatcacccg cgtggacgtt gccaccgagg acgtgcaggg cttttacgag
    23821 cagcaggggt ggggaccaaa ttttgagccg tacacccgga gcgatatctt cgatccccgc
    23881 accgtacttc agggcggctc attcagcttc aggcaacagt ttcctgcgaa tcagccagtc
    23941 gagattcggg gccgggcctt tgccggtgac cggggaatta gctcgatcga tatcagcacc
    24001 gatggcggag aaacgtggac gcgggcggag atttactacg caggcaccag gctcacctgg
    24061 tcactctggc gctataggtg gacaccgccg aagtccggag aatatgtcat cacaacgcgc
    24121 gccttcgacg cggctggcga acctcaaacg caggaggtgc gcggcatcgt cccacagggc
    24181 gcatccggct accacaaggt gacgatgaca gtcctgtaaa gaaaagaggg ccgcaatgtt
    24241 gcggccccct tcttcacaag tcccgctttt gtgcgggaat tatgtcccga gcttgcggaa
    24301 gtgtacgagg ttggtgcggc cggcttcgtg ggacattggc aaggatccaa cgattagcgc
    24361 gtactcgccg tcctcggcca acctttggtc gatgatcaac tgacccgcct tccggaaggt
    24421 tgcatcctgg tctcgctcga cttccaacac cactgtctca attccccaga caaggttgag
    24481 ccgccgcgcc acccgctcat tatcggcaac ggcgatgatc ggtgggaatg ggcggtactt
    24541 ggctatccgt cgggcggcgg cacctgtctt tgtaaagacg acaagatgtt gcaccggcgt
    24601 ctggtcggaa agcgcgaacg cagcccgtgc gatcgcatcg gcaaactgcg actccggatc
    24661 gtcctcagcc gcaaatttct gcttggccgc ctccgagcga accgtgcctt ccttctccac
    24721 ctcgcggatg atccggtcca tcgtccggac cgcttcgatc gggtacttac cgaccgcggt
    24781 ctctgcggac agcatcacgg cgtcggtacc atcccaaaca gcgtttgcga catcgctggt
    24841 ctccgcgcgg gtcgccaccg gctgggtgat catcgattcc agcatctgcg tcgctgtaat
    24901 caccgggacg cccacgcggt ttgccacgcc gatgatcttt ttctgcgcgc ggggaacacg
    24961 ctccggcggc agctgtaccc caaggtcccc tcgcgcaacc atcacgccgt ccgccgcctc
    25021 gacgatctcg ttgatgttca tcagggcctg tgggcgctcg atcttggcga ccaaccccgg
    25081 cacgatgtcg ccatgctggc ttgcgatctg tcttgctaga tgcagatcgc tggcatcgtt
    25141 aaggaagctc agtcccagga agtcgacacc atgcttgacg gcaaaggcga cgtcttcctt
    25201 gtcggcgtcc gtaagcgcgc cacctttaat cggtgcaccc ggcaaggtca cgccctgctt
    25261 gccaaggagc tgaccacctc gcgcgaccat cgccatggca tgatcgccct tgatcgattc
    25321 aacgagaagc tcgatcagcc cgtcggcgat cagcacccgc gaaccctcgg acatcacttc
    25381 gaacaggttc gggaagttga tgccgatacg gccgggcacc gtttctacgg gcccggcctc
    25441 gagacgaatg cgactgccgc gcgcgacagc aacgctatct tcaccctcgg gaagcggacc
    25501 ggtccggatc ttcaggcccc ggagatcgaa gagaatcggc acacgcttgc caattgcttc
    25561 tgccgcctcg cgcactttgg taatgagctc agcacgctct tccgcggaac catgagccgc
    25621 attgacgcgc acgacatcca cgcccgcctc catcaggtcc tggaggcgtg caggctcgct
    25681 cacggccgga cccagtgtgg cgacaatctt gactcgtcgt tccataaaac agcctctatc
    25741 gaatatgtag ccgtcgtgaa aaacgatgcg tgccgctccc accccgggga acaatttgcc
    25801 gcatcggctt catggtacag gaatctgtac gggtacgtag gaaccacacc gaagtgggag
    25861 caccgcatca gggtggcgct cccactttcc ggtcatccat ccctcgtcag gcgcttactc
    25921 gtatcgcagt gcctctgccg gaagaatccg cccggcctgt cgcgctggca ggtaggacat
    25981 aagcagtgcc gcgaagattg ccaccgccag cgtaacgata atcgtgcccc aaggggcgga
    26041 gaactggatg tcgacacctt gggagaactc gtcgctcgtg aggagcaccc atgaaaggat
    26101 gagtccggtg atggtgccgg acagcgtgcc gagaacgacg acaattgccg actcgatcac
    26161 gaatgcctgt gcgatgctgc cgctctggaa acccagtgcc cgcagcatgc cgatctgctg
    26221 gcggcgttcg acgaccgcac ggtacgcgat aacgccgacc gcggcgatgc ccacgatcat
    26281 gccgagaccc atgaagcctt gcagcacgta catgaacgtc tgctgctgtg cctggctctc
    26341 ctccatctcc tgctcgatat ccacgccctg cgcgccatac ggcaacagca cgcgctcgat
    26401 gttcgaggcg aaagcgcttg catccgcgcc atcggcgaga tcgatgaagt acgaggttgc
    26461 gctcgaacta ctgccgccca tcaccgcgtt tactgtgggc ggaccgacgt aggagaagaa
    26521 gagcattgac aagctactgt cgagcacgcc gatcaccttc atctcccgaa cctcacccac
    26581 cggaacccgt acctccaccg tggctggcgc aaacgggccc tcggtcggaa caccagccag
    26641 attaggagtg ggaggaccac cgaaattggc cggggcgtcc tcggcgtagg cagggttgac
    26701 gacgacgaca ttcggttctg tcctgagcgc ctcgatgatg gcctcgtccg tctcgtagcc
    26761 ctccgcatgc gcctggaatt ccagttctgc tgaatcgagg tatccctcat ccatccccaa
    26821 aacgatgaat gaaagccatt cacccccctc gtccttcacc tcctccaggt tgtccataga
    26881 caccattttg ccgacgcccc tgaactgtgt ggtatcgaca ccttcccgct ccagcgtgtc
    26941 ccggaaatca ccaagtggat tctcgtcggg cacatcgacc cgaatctcca tgccggcgtt
    27001 tgcctgcttg ctgaggaaag cattcgcgaa gttattgttg atcgcggcga ccatgacgag
    27061 cgagaagacg ataaggctga acatagcgat cgtcataccg gcccggccct tgttcgcgcc
    27121 gggatacgcc accgcgagcc gcacagacgg tagcatcgac ctcaatgcgc cgccgtagcg
    27181 cgtaatgacc gagaggatcg catccaggtt ctgcacgatc accagtgtcg atgcggcgac
    27241 aatgcagatg cccgaaacga agaacatctc gatattgccg ctcatctcgc cgaagaccgg
    27301 cgtgaagaga tcgctcggca ggagccagaa cagcagaaga taaatcccgg cgatcgtgag
    27361 gacaagacgc gagttcgccc cgaagaatgt caggatggca gcgatgccca acgggatcag
    27421 tgtcatgccg atcgtgaagg gcgcctgctg gtcgacattc attcccagga aggcgagcag
    27481 cactccagcg gccagcatca ccactcccca gaacaactgc ttgcggttcc gatgggcgtt
    27541 ataggaatcc gggatatccc ggatggctgc gaccacgttc aagcggctca cgcgccacga
    27601 agagaccgcg accgccagaa aggtgatcac gacaccaagg ctgtacgcga tcaccaggga
    27661 acgaggagtg acgtagagct cgatcgagaa caggccttcg gcaaggccgg aaaatccctg
    27721 tgcgattgcc cacgtagcga gtacgccgag ccccgtaccg atcaggccgg agagcaatgt
    27781 atatcccgct ccttcggcaa gaaactgctg aatcaattgg cgtcgctgcg caccgacggc
    27841 gcgctccata cccatctcgg cccgacgctc ggccgcgagc atggtgaaga tcaggacgat
    27901 cagcaacacg ccaacgccga ttgagaagag gccgaacacg acgaagaagg ttacaaagat
    27961 gttcgcgatg agctccgcct gctcgacatt gtcctgcttg atcgtgacaa ccccgagatc
    28021 ttcctccgcc agtccggtgc gcaggaggga cgtgacttcc tccgtatatt ccagcccttc
    28081 gcgagtgcca ccggcattgg agatgccaat accacttacc cgtccctcct gtccagtgat
    28141 ctcctgcagt cgacccagat ccatcagcat ggacgccgtg cccgggttga tgcttcccgt
    28201 cagtacggta tctggcagaa caccccgcac ctcggcgaaa tagggttcgt tccccgagat
    28261 gtaggcaatg gtgtcgccag tggtcaggcc aagatcttca gccgtctcct tccccacata
    28321 gactgcgttg tcacccagcg tcgagacatc gaccggattt ccattcaggt cctgcatgcc
    28381 gccgatatct tcgattgttg tctggtcaag acccgcgatg aagaccgttg ggttcgacgc
    28441 cgtcgccgcc cggaggatgt cgtcaatcga ctgcaggttg tatggataac tatcgcccag
    28501 attgagcact ggcgactggc tcaggaggat accggcaacg gcatcaacct tgtcactgtc
    28561 cccgaccgca gcacgaacgg tttccacatt cgactcgggc atggtctcgg tcaggatagt
    28621 tccaatactg gcattaccct cgtcatcggt cgcggcgaga acgatctcgt ccagcggccc
    28681 cagggttgtc agtaccgcac tgttcaccga gcggttgagg gtatcgcccg tgcccagcgc
    28741 tgccgagatg atcagggtcg cgagcatgag tccgatgatg atcagcgtcg tctgcgcctt
    28801 ccggcggggg atgttcctga cgcccagctt aaaaatcacc ggccgccgcc aggcgataaa
    28861 ggcaacggtc agcaggcagg tgccaaggag gacgagcatc accagcatga tgttggtcat
    28921 cgggatgcca aagagctttt ccacgcgatt aatatccttg tgagggaccg ggaccagccc
    28981 tcatggggcc gccctaggag acgacggaaa atgcctcatt gccgcgcaca gccatcgcct
    29041 gagtagtgga tcggtcgggg aactcctcac cgacgacttc accgtcgcgc atctggacaa
    29101 tgcggtggca gcgctggccg acacctggat catgggtgac gaggacgatg gtgaggttct
    29161 cgtcgctatt cagcttttgg aggagatcca tgatgtcccc ggcagaacgc gaatccagcg
    29221 caccggttgg ctcgtccgcc cagataattg ccgggttgtt gaccagcgac cgcgcgatgg
    29281 tcacgcgttg ccgctggcca ccggaaagtt ccgccggtcg atgtcccgac caatcccgaa
    29341 gaccaacccg ctccagcgcc tcaagcgact tcgttcgtgc ttctgccgac ttgacattcg
    29401 agacgagcaa tggcagctcg acgttctcga ccgcactcag cacaggcagc aggttataaa
    29461 cctggaagat gaagcccatg cggcgcgcgc ggtaattggt tcgcttcttg tcgctcatct
    29521 cggcgaggtt ctcgccttca atgaagatcg tgccgctatc cacgtcatcc agacccgaca
    29581 ggcagttcag aagtgtggtc ttgccgcaac ctgagggacc catgaccccg accatctcgc
    29641 cccgctgaac ctcgaaggtg atccccttga gcgcctgaac cttgttggtt ccagtgtcat
    29701 aggatttaac tacattgttc gcggaaatga tcgtgttctc gcccacctca gccggcactg
    29761 acattttctt cttgccaagt cgcatggcac agctccttct gcatgacaga cacactcggg
    29821 cgcacgctgg tttctgcccc aacgcacccg gtcctatccc tgtttccggg attgttctct
    29881 atatgacccc gcacgatgat acatcgtgag gcgcaaggtg tgcttctcaa aaccactgcc
    29941 acactctggc agaagtgcat aaaccctgtg taccaccaga aacgtctgat acccgaacct
    30001 gaggggccac gcccattatc ttgtgggata tcatcccctg tactaccgtg acgggcagtg
    30061 acatcttgtc aggggagata tcaggggacc atgggctggc tacgtgatct gattgcgttt
    30121 attcaggaaa gaccagagcg ctttctggag ctggtgctgg cacacttgcg cctggcatcg
    30181 acggcacttc tcatcgcttc gctcatctac attcccctcg gtatcctctt ctcccggatg
    30241 ggacgaaccg gcgcgaatgc cattggtctc ctgtcgtcaa ttcgtgtgat cccctctctt
    30301 gcgctggtct tcctcttcta cccctggctc ggattcggca cgttaccggc gctagtggcg
    30361 ctcatcgttc tcgctgctcc cgcattggcg gtcaacacgc acgcggggtt ggcaaacgta
    30421 gacgccttca cccttgaggc ggcaaggggc gttggcatga acagtgcaca ggtttttctg
    30481 caggtgcaat tgcctctcgc ccttccggtc attgtggctg gcctgcggct ggcagctgtg
    30541 gagcttcttg ccagcgcgac gttagcatcg ttcgttgggg caaagacgct ggggcaatac
    30601 attttgaccg gtatctcgtt gctggatacc acctatttac tagccggggg gatccccatc
    30661 gtgatactgg tgttactcgc tgacctgatg ttcagtggga tcgagcgcct ggtgacgcca
    30721 ccaggtcact aaggctggaa acagaacaag gaagggacga tttcacaatg attacccgtc
    30781 gttcactcgc caaaactacc gctgccgcgc cgatcgtcgg cgcgggtttc tttgcggctt
    30841 tccagcctgc ggcctttgcc cagaatggag aacctgtcgt tgtcggttcg aaggatttca
    30901 ccgagcagct catcctcggg cacatgtatc tccaacttct tgaggatgcc ggcattcccg
    30961 cggaagagcg tctcaatctc ggcggcacac agatcgccca tgaagccctg gtcaatggcg
    31021 aaatctcgct ctacccggaa tacacaggga ccgcactttc cgaagtgctc cagattccgc
    31081 tcgaagaggt tcaggcctct gcggtaagct ctatggcttc tcccatggca ggtgcagaat
    31141 cggcaacgcc ggcggcgagc agcgatctct cgcaagccgt ctacgagctg gtgaaaaccg
    31201 agtacgagga ccagttcggg ttaacctggc tggaacgctc acccgcgaac aacacgcagg
    31261 ccctggccgt gaagcgcgcc ttctccgaag agaccggcat cacgacgatc tcccagcttg
    31321 ccgagaacgc aagtgagtac accatctccg cgcccgccga tttcccggag cgtgaggatg
    31381 ggcttctggg gctggtccgc gtctacggtg agaacttcag tgagatggag gtcctgccag
    31441 tcacgccggg cctgaagtac caggcattgc tggacgacga agccgaggtg gttctcgcct
    31501 ttggtaccga cgggcaaatc agtggttatg acctcgtggt actcgaggac gatctgggcc
    31561 tctggccgcc gtacaacgtc gccccggtgg tccgctctga ggtgttggaa gcctatccgg
    31621 agatcgctga gcggttcaac gccgtgacca cgaccttgac cgacgacgta ctctcctccc
    31681 tcaactggat ggtcgacggc gacgagaaga tggagccagc tgatgtcgct gtgcagtacc
    31741 tcacggaaaa cggattcatc gaagaataag gtcacaacct gctaacactt ggtatgctga
    31801 cggcgcgaga catgtcccgc gccgtcgttg cgtattcatc tccggagctc cgctaccgtt
    31861 ttggctatca accagcgatc cggcacgtcc ggaagttcga tctccatcca tgcactgacc
    31921 aagcgctacc ccgggtcctc tgcagcggcc gtcaatgcgg ttgacctcga cattgcccct
    31981 ggcgagctta tcgtgcttct gggaccatcc ggctgcggca agacgacgct gctcaagatg
    32041 atcaaccgca tctatgaacc aacttccggg tccatcgtca tcgatggcgt tgaggtccag
    32101 agcctgaaag cttccgagct tcgacggcac ataggctacg tcatccagca gaccggtctc
    32161 ttcccccata tgcgggtcgc cgagaacgtc gcggttgtac cgaagctcct gaaatgggac
    32221 cgcgaccgaa ccagggagcg cgtgagccaa cttctggagc ttaccggtct tccgcccgag
    32281 atctatgcaa accgctatcc gtcgcaactc tcgggcgggg aacaacagcg ggttggcctc
    32341 gcgcgagcgc ttgccgccga accgcccacg atgctgatgg acgagccatt cggcgcgctc
    32401 gacgccatca cccggacacg gcttcaggac gagctgaaac gtattcacaa gcagttctca
    32461 cagaccattc tctttgtcac gcatgatatt gaggaagcgg ttcgccttgc tgatcggatc
    32521 gtcgtcatgc gggagggccg catcgttcag gtaggcagcc cactggagat cgtcagggag
    32581 ccggcagacc agttcgtctc cgatctcgtc gggtctcaag acgtgctgcg tcgactcagc
    32641 ctgatgcctg cccgcactgc gatggagccg ctcaacggca ccccaccaga cctggcccaa
    32701 tcgatttccg gggaagtctt cctccgggaa gcgctgagca tgctcatgga atccggcgat
    32761 cccttcatcg cggtcaccgg cagcgacggc acagcgatcg ggaccttgag catgacatcc
    32821 atccagcggg ccgccgcccc tcctgacgcg ccaaaccggt aaccatatgg atcttgggta
    32881 tttcttcgac aacctggacg aaatccttgc gctctcgctg gagcatctgc gcatatcgct
    32941 gacagcgctc atgatcgcac ttcccgtggc gttggtcatc tccgtcatcg gtgtctggtt
    33001 tcgaccggct cgacagcccc tgctatggac ggtgggggcg ctttacacca tcccaagcct
    33061 ggcgatgctg gcgttcctca tcccgtcaca gggtcttggt cttcgcccga ccctgatcct
    33121 gcttgtcacg tatgcgcagg tctttctcgt gcggaatatc gtcactggac tgacgggcgt
    33181 cgatcccgcc gtgctagagg cagcccgcgg tgtgggaatg accccgcttc aggtgcttag
    33241 ccgcgtatgg ctcccgattg cgctcccggt cattcttgcc ggtgtacgca tcgcgctcgt
    33301 taccattatc ggtttggcga ttctcggtgg ttgggtcacg gcaggtggac tgggagaact
    33361 cctcttctcg gggatcaaca cgaaccatcc agcgaaggta ttggctggcc tgatcgcgat
    33421 cgtctccctc gccttgctga ccgacctcgc gatacgcctg ctcgaatcct tcacatccgt
    33481 cgcccgtgcc cgccgggcgg gaaaccgcgg gtgacgctcc agggcagggg tgcgaaaaga
    33541 gggcgggaat gacttcccgc cctcttttct ccagttttgg cttttcctag cgtcggttca
    33601 gaatcaggtt cttcagccag accaggatcg ctgcgaagat cgctgcgatg acgattgaca
    33661 cgatgatatt ggcgtcagcg aaaatctcgt ctgcgacgat accagcgaca aacgcaccta
    33721 ctaaaccgag tatcaggtct ccccagatac tacgatcggt ccgcgtgatc accccggcta
    33781 accatcccgc cgcaattccg acgacgatgt aataaagaaa cgtccccatc tgttcatccc
    33841 tctccgcatc gcgcgggctt gtcggtctgc cacccactcc gggcagtccc cacggtgtcc
    33901 accagcatgg accaaaccac gccgccttat gcagggacca tgccacagta tcctgagtct
    33961 gcctcaggag tttttttcct cggtgtctgg tggttgccac gcatggagcc ttcgcagcgc
    34021 ttccgcccgc ccctcctccg tatcctggag gtcaagcgcc cgtcctgatt catctgattc
    34081 gccatcagtg gcggttacgt cgcccggcag tggctcctcg tcgctctcaa ggtcgttcac
    34141 atcaggtgtg ggcgaagaat cgtccagttg cacgtcttcc atggggcggt cttcgttcaa
    34201 actgatgtgg atcgtgtcga ccgtctgggg ttcgtcattc gaaactggct ggaagtcggg
    34261 tatcactgca gtgagacggt cgtctggcgc ctcgatatcc ggtgggtcgc cgtacgcagc
    34321 cagctcttca gcttcctctg gaacctcatt cccgttgaca tcagcgaccg acgggcctct
    34381 gtcctccaga atctccgctg aagcgtcctc atggacctgt tggaccgtca cgacatcatc
    34441 cggatgatgg agcaggaact tcgcatagcg ggattccagc tcgtcatact cagaaatgtc
    34501 gtcattggta acagcggcaa agccgtcgtg gtcctcaccg atccctggct cttcgtagat
    34561 atcgtcgtcg ggttcctggg taccggaaat tccgtccgcc tcacggccgt caatggccat
    34621 ggcagctgtc ggttcgaccc tttccattcc tctggcggga actactgcgg gctcctccct
    34681 tcgttcggga agaatgccat gtgtcccata tcggcccaca tagagccatg tccagaccgc
    34741 aaccgcgagc aggatcaagg cgaagcaaat cagggcaatc aatgcgcccg tttcgtctcc
    34801 gggattgatc aggatgagag ctacggtgac aagaatgagg gacgcgccgg cggtgatgat
    34861 aactgaaccg agggcggcgg cgaagcgtcg ccaggagatc cgcgccagta tggcgtcggc
    34921 acccatgacg gagccgtcac ctgaatcctg atagcgcgca ccagggccga acgtccgcag
    34981 tgcccagccg agcagcatca aggttgcccc atatcctgtg ccaaagacga cgtaatccac
    35041 cctgactccc tacgacgctc gacaatgaca ttcgcacgtt ctccaccgct tcggcggatg
    35101 ggaggacgtc gttcatcaac cctctgatgt ctcccgcggc tagtctacag cggaagaaat
    35161 tgctgaggca tgtgcgcgta cgatgctgcg tagtccgccg caacggtagg agtggccact
    35221 tccccgtgcc actccgagcc taccaacaat gcagtggcga gccacctcac tacacggtag
    35281 ggacgcgtac cgtgcgacct gcctaccttc tcaaccgctc cagaagcata gcgtagccct
    35341 cgcggccacg ataaaaatct gcaacgctgt accactcgtt cggcgcgtga gccttgctat
    35401 ccggtgccgc ccacgccatg gtgattgtct cgacaccgag ctcgtcctgg aagatgcctg
    35461 tggccggcac agtaccacca ttgcggacaa tcaccgggtc cttgccatag agctccgtca
    35521 ggacgtcctt tactgcgctg aagaccggat cttcgcggtt tacggaatat ggacgggcag
    35581 aaccaggcaa tggaacgaca tcgactttcg ctcccggagg gcgatgtgct tcacaatgtg
    35641 cccggatcag ctcgacgact tccaaaggat cctggtcagc gacaagtcga caggtcacct
    35701 tgagatgggc ctcgcgcggc gtcacggtct tgacgccgtc accctggaaa ccgccccaga
    35761 tgccgttgag atcgagtgtt ggccgaaccc actggcgctc tcgtggcgtg tatcccgcct
    35821 caccccaaag tgaatcgata tccagctttt gcttttcctc atcctcgtca atcgggagcc
    35881 gcgcgatctc ctccttctcg gcctcggaga gttcgcggac cgcatcgtag aatcccgcca
    35941 cctgcacccg gccgtcctca tcatggaagg tggcagcaag ctgcaccatt gactgcaccg
    36001 cgttggggac ccacgctccg tacgacccgg agtggaggtc cgtgtttgcg gtcgtgagat
    36061 tgatctggac gccccccagg ccctttaggg agaccgccag cgacggtgta tcggaggtga
    36121 actgtcctcc gtcagcggaa atcaccgcgt cggcgtcaag gaagtccttg tgctggcgca
    36181 gaatgtcccg gaagtggggg cttccgatct cctcctcgcc ctcgaagatg aagcttacgt
    36241 tgaccggagg ttgcccaccg ttagcgactg ccgccgcctc gacaccctgg atagccgtga
    36301 gcaggttgcc cttcatgtcg gaagagccgc gggcaacgat cgtgccgtcc gcaacgaccg
    36361 gatcgaatgg cgccgaatcc cagagctcaa gcggttcggc cggctggaca tcgaagtgcc
    36421 cgtagatcag gatcgtcggc tggtcatccg cgactcgcca ccgggcgatg acagctggat
    36481 gccctgctga cggcgcgatc tccacctccg gtacgccggc ggcacgaagt cgattcgcca
    36541 cccactccgc tgagtcgcgc agatcgccag tgcgctcggg atcagtgctg ataccaggca
    36601 tccgaaggaa ggtcaggagt tcctccagat gttgcccttc ttttgtcttc aggtcgtctt
    36661 tccagtggat ctctaccatc gttcctgcaa acctccgggg cacatggccc tgcatgattg
    36721 ttttccgccg ccatcatcgc agattccccc cttgcacgaa atcgcgctgg tagccggtct
    36781 tgccaaaagc ggtacggctt ctcaacgcgt ttcgggaagg tctactcgcg atctattgca
    36841 ttttggccac attgcgctat gatcgatttg ggtccatatc gctggtgaca gcgtgactat
    36901 tcgtagcctg acacacgttg ggaagacatc taccggccaa cggttggttt gatgtgcccc
    36961 gggtttggtt gggtgtgcct gcttgaggca ctaccggaac tcaggcgctc tttgggggag
    37021 cgcgctgata ccgagactat aggaacagat ggacacagta cagataatcg accagaacca
    37081 ggaagacgag cgcctcgttg caagagcgca acagggtgat ctcgatgcgt tcaaccgatt
    37141 ggtggtgcgt catgagcgcg ccctctacaa cgtttgcctt cggctgctgc gcgacccttc
    37201 gcaggccgag gacgcaacac aggacgcatt cattcgcgca tggggagcaa tcgacagctt
    37261 ccgtggtggc caggtccgtc cctggttact tcgtatcgct acaaaccggg cctatgatct
    37321 cctgcgatca aagtcccgcc gaccggcaga atcactggat tcacaaccgt tcgaaatcga
    37381 accagagtgg acatcccagg cccctggagc cgagcaaccg gaaaccttcg cgactcgctc
    37441 cgaactgagt gcccatcttg agcacgccct tgctgtcctt cccgaagacc agcgactggc
    37501 gatcatcctt tcggacgtgc agggctacgc ctacgaagag atcgcagagg tgatgaatgt
    37561 tgccgtcggc acaatcaagt cccgcattag ccggggccgc tcgcgcctcc gggctgtgct
    37621 tcaaggaagc gagagagatc gggaactgac agagagcttc ggtcgtctta acagcgtgta
    37681 gttctactcc tgactacacg aaggttagat gagcgggccc gcctgtagct gtgtcagagc
    37741 accacgtccg gcttcacatt tctccctgga gaatggaagc aacgctggca cagccggggg
    37801 gacacgtcgg atcaggatct ccatgatcac gtatatgcac accaccaacc atctgagcga
    37861 cgagttgctg aacagctggg tcgacggcgt tgttaccgcc gatgagcgca gcatcatctg
    37921 tactcatctt gagcactgcc aactctgccg cgatgagctt gaatcgcttc gcagcgtcaa
    37981 acgactcctc tccgatcttc cacaggttcg gcttcctcga tctttccagc tcacacctga
    38041 gcaggcgcgt gaccgtgata ccggaaccga atcgacggct catgcgtctg agaatgagaa
    38101 cccggcgcac ctgcaagggt cgctcggcga agtcatagac gagggccagg cagattcggg
    38161 cagcgaggtt gcggtgactg gtaaggcact ggccttcccc gaggtttccc ccagggtgct
    38221 tgccgccatt gccttcactt tggccgtcgt tctggctggg attcgggtcg tagcacatcg
    38281 ggccggtcgg ttacgcccgg caccatagcc ggtcatccac gcgtttgatg gcggatgatc
    38341 ccgaaggagt catgtatgca gtccggcatc aaatctcgtc ccagtggagc gttgctcttt
    38401 ccctttctca tggcccttat cgttgcgacg gtggcactct tgccgtctgt cgcggctgca
    38461 cagtcaaccc cggtcgccga ggagacggtt cggacgatca cggtgaacgg cacgggagtc
    38521 gtctccacaa cgcctgatac tgcaagcgtc atgcttggca ttcggagcac aaatgaatcg
    38581 ctccagaccg cccaaggtga cgtgacacgg cgcctcactg gagtcacgca gacgctcatc
    38641 gaggccggta ttgcagagga agatattacg accgcgcagt acaccatcac tccgattccg
    38701 gaatatgacc gcgatggcaa ttacaaggga atccagcaat atgaggtctc tgtcgggctt
    38761 gccgtcacag tccacgatct ggatttgctc ggaaccttgc tcgattcgac cgtcgacgcc
    38821 ggcgtgaatg atgtctgggg catctccatg tatgtcgatg acacatcggc cgcggcgagc
    38881 gaggcacgaa cggctgccat ggcggatgca cgggcgcggg ccgaaga
//
LOCUS       DASYWM010000101         9427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_472687, whole genome shotgun sequence.
ACCESSION   DASYWM010000101 DASYWM010000000
VERSION     DASYWM010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472687"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGR29_04150"
     CDS             <1..243
                     /locus_tag="VGR29_04150"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEV2072815.1"
                     /translation="ANLPVLSEGRSYQLWYSTDSPTTPIPGGTFRVDDTGQGFMLIPS
                     DVGMIASIAVTEEPVGGSQAPSGPVLLASDVSGARG"
     gene            452..2023
                     /locus_tag="VGR29_04155"
     CDS             452..2023
                     /locus_tag="VGR29_04155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2072816.1"
                     /translation="MPPPATAADVLWLMVAILLSLALRIPFFKIPMIHDEGGYAYAAR
                     GWIEGTGRLYDDLWISRPQGIFVLYGLVFRFLGEGVLALRFTAWIFVALTVLAVWFFA
                     RRWAATRTANLAAAITAVVLALPNLEGFTANAEIFMGLPIAFAAFWLVRQAQLRWSMW
                     QLCGVGVLIGLATVLKPSGIVMAFTALAFIAMVGEDVSLQQRLHRCGAVLGGMALVGI
                     VTLAHGWYLGWSAFIYATVTYRLTLQSSATVSAMRHLISLGRLAWTIVALLSLVYVIF
                     AFRTHISVCPPAHLQGAAGSWQLKETVPDRPGHLRLFITSRRSLNDGETASLMIQLWS
                     IGAIAGIAMGGDWWSHYLLQIAPPFALWLAWNLERIVGVLRHWQRWLFVAIASGLLLL
                     PYEILVDGPEELLNDHPGYPAQAEVSRYIQEHSDPQDRIYVAFDQAAIYYLSDRKPAY
                     RHLYDQELRALPNSYADIIEVIRGDDRPLYIVSTLHPGPFPDDSRTFWREVGQYYEIE
                     TTIGGVPIYRAKDAP"
     gene            complement(2078..3967)
                     /locus_tag="VGR29_04160"
     CDS             complement(2078..3967)
                     /locus_tag="VGR29_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV2072817.1"
                     /translation="MTTLQFDQSPSDFEPIAYLDGYLPIEDYGIIGDCSTAALVGRDG
                     TISWLCAPRFDSEPIFCSILDHELGGAFTVDGGQIIGARHRYIGDTPILITELKLESG
                     VLRITDLMPTQAGADLNIEGNVNVGELLRCVEIVEGTITVKARISIRGGCEAERHENG
                     YRLSSPCYPGMDLVVESSCEFSGTSGEWTLTRGESVTFCLRWNGGTGASSVDAPVAAV
                     TNTTETWLRWMRAFTYEGPNREHVKRSALTIKLLDYLPTGALVAAPTTSLPEQIGGER
                     NWDYRYVWVRDAAFTVNALRRIGLDREAWQFLTWVLGTAQGTQINPMYTLYGEPRIPE
                     SLDPELSGYRGSAPVRWGNAASSQIQHDVYGELVDCAYQWANRGGMVNDKLWGRLHEL
                     VERAGEVWRTPDAGIWEVRTPGRIQTYSAGLCHVALDRGIRLVRRFELSGNVEEWERK
                     AQRIQRTILEDAWDEEQQFLTQTIRTTEDGQGQELGHLDAALLSLPLRRAIPGTHSRM
                     VKTAAAIEDQLGAGDGLLYRYLHDKSPDGLSGHEGAFVLCSFWMIDNLILQGRLQEAA
                     DRYDRMCARTNRLGLLPEEIDPGTGRFLGNFPQAFSHIGLITSGVNIGSAMESKDLAI
                     SSAES"
     gene            complement(4022..4369)
                     /gene="mnhG"
                     /locus_tag="VGR29_04165"
     CDS             complement(4022..4369)
                     /gene="mnhG"
                     /locus_tag="VGR29_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEV2072818.1"
                     /translation="MRAYVYDAMLIVSLLVMTLGIIGIWRMPDVYTKLHGASKSVFLG
                     VVVLALSATVVAEETMVYRVILLIMLVVVTTPIASHVVARAAYIMHEQMETPGAVDES
                     HTLRKEQEPTWRL"
     gene            complement(4372..4638)
                     /locus_tag="VGR29_04170"
     CDS             complement(4372..4638)
                     /locus_tag="VGR29_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HEV2072819.1"
                     /translation="MHEVVFYIAMIWLVILFCICVLMIVRAKSGLVRVLSLDTLTLVL
                     VALLIVYSVTTGTAYYLDAAVVLSLISFISVIAAARYHAERRLF"
     gene            complement(4631..5173)
                     /locus_tag="VGR29_04175"
     CDS             complement(4631..5173)
                     /locus_tag="VGR29_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEV2072820.1"
                     /translation="MILVIGILASSVLLFGLMTADFTWQNLVLGFAISIGLLWGFRKQ
                     VIPRPLPPSGFALHLVIYAPVLFWYLLIDILKGTWVVSLTTLGIRPLQKPGIVTLPIG
                     HHSPYGVGPVGYFVTLSPGSFLVEVDWDAREMLVHVIDASNPDAVRRDAEKYYRLWEY
                     GNYAPRELPGSDREEGEPHA"
     gene            complement(5170..6540)
                     /locus_tag="VGR29_04180"
     CDS             complement(5170..6540)
                     /locus_tag="VGR29_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEV2072821.1"
                     /translation="MIALPVAVLWFGGFVLAVLDGTRKPAAWLSVAVLASAILSTMAL
                     GITVYQDGPMSLAVGGWSFGVGITLRVDMLSIVFALLSQGVLLAALVYEVVNEVGARI
                     YPAVVLFMGLGLTGLVFTGDAFNFYVFFEISMISAYVLSSYGESPRQFRAAFVFITVN
                     LLGSVFFLIGISAIYHTTGFLDMYRIRDQMPLVQENPAILSATIIFVAFSIKLGLFPF
                     HFWLPAVYTGTRPSVAAILSGALANIGTYGLIRFGADIFPRELRQAAPVLIALGTASI
                     IYGAIQALSRHATNEVLAYSAIGQVGYILIALGIGGPAGYTAAILYTVLNSTNKALLF
                     LSGSLRGWLAGAAFAIGAFSVAGVPPASGFLGKMALFQVGIAEDRPMLVALIFLGGAL
                     SFVYMFQLYRRRTAEIEAHDSSPWGAQVVVVMLALVILGIGVWPDPLLNLVERAALSL
                     PEAQVP"
     gene            complement(6537..6980)
                     /locus_tag="VGR29_04185"
     CDS             complement(6537..6980)
                     /locus_tag="VGR29_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEV2072822.1"
                     /translation="MILVISLAIAVMFAAGAYLMLKRDLIRLIAGMILIGNAANLFIM
                     ASGLRRGAAPILPVEEPTADPLVQAMVLTAIVISFATAALALALVYRVYISHYSVDLS
                     RLSAAEEQQAERDEPMTEDLREGVRDDTDGEASPDDRRARAEAIR"
     gene            complement(6977..7513)
                     /locus_tag="VGR29_04190"
     CDS             complement(6977..7513)
                     /locus_tag="VGR29_04190"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HEV2072823.1"
                     /translation="MPARNPQKHAGMLQSGARIILLPSWIIAIAIMLKGYADVGDGFS
                     AGVIAALGVLLQGLAFGTEELEQITLSRIAPILSFVGLGLALLTAFVPILFGEDLFTH
                     WPGINEHAVHFGVLEFITPVVFDIGVFLIVYGFCVGGLHAIAREEVRQVKLRDRGRYL
                     RSNAHGGSLADESGEAPL"
     gene            complement(7513..>9427)
                     /gene="mbhE"
                     /locus_tag="VGR29_04195"
     CDS             complement(7513..>9427)
                     /gene="mbhE"
                     /locus_tag="VGR29_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HEV2072824.1"
                     /translation="DLILIFVFWDLTAIASYFLIGFDRAEEESRSSAQMALLVTGITA
                     IFVLLGALALFQIYGTFSLPEIISASNGGRNTGIAVGLIVLGALAKSAQVPMHFWLPR
                     AMAAPTPVSAYLHSAAMVAAGVFLIGRVYPLVEQYGWMLNALLIIGFASMAVGGIIAL
                     TRRVLKQILAYSTISQYGYVVAMFGMGGEEGVAGATFYVLAHALAKSALFMTAGAVTE
                     ATGKKNLPDVGGLMRRMPLLAAGSGLAAAGIIALPLAIGFFKDELFFAAAYARGTEVA
                     VMSVLGAALTFAYILRFWWQTFMGPLRAETRAVSGYLVWPVVVLGIATLVGGVWTAPA
                     TGIVDRAASVSADAPIHLHIAYHLDARHENVMAIATWVTGLLIYLTRRIWWPAALGVA
                     HLGSYFGPARLYYAGMQGLNQLSDSVHRIEVRDLRSRVATILLPAGLLVGLALIVTPN
                     AVRSFDMGSLNRGDIPIIAMLVASVASALVVVVVRDHFRLAISLSSVGFSLAGVYAFM
                     GAPDVTLVALVVETVLSMFMIGMLVLMPRPILRFETEVRAERRYLRRDLILAMVAASM
                     AFFVVWGALSRPSPSTEVIDSQTVLTAAVHGKDIVTVILADFRGFDTMGEATVIALTL
                     LGVISLLRTGRLR"
BASE COUNT         2078 a         2961 c         2693 g         1695 t
ORIGIN      
        1 gccaacctac ctgttctctc ggaaggtcgt tcctaccagc tctggtattc cacagattcc
       61 ccgaccaccc ccattcccgg tggtacgttt cgggtggacg atacgggaca gggatttatg
      121 ctgatcccgt ccgatgtcgg catgatcgcg tcgatcgctg tcacggagga gccggtcgga
      181 ggtagccagg caccaagtgg accggtgctt cttgcgagcg atgtttcggg ggcccgcggc
      241 taggcaacgc gagaacagtg gatttcccga gcacgtttgc cggtgtggct cggatgtcag
      301 ggtacacttg ggcagcatca acggatcaac ctcatccggt tgaaagcagg cactcgtgac
      361 catcagggcc ccgcatccac caccatcccg gtcgccatca tccggctcag caccgcctct
      421 gccaagccct gacctacaag gtccgccacg gatgccccct cccgcgaccg cggccgatgt
      481 tctctggctt atggtggcga tcctgctcag cctggcccta cgtatcccct tcttcaagat
      541 ccccatgatt cacgatgagg gcggttacgc atacgcggcc cgtggttgga ttgaaggaac
      601 ggggcgcctg tatgatgatc tctggattag ccggccgcag ggcatcttcg tgctctatgg
      661 cctggtcttc cggttcctcg gtgagggcgt cctggccttg cgcttcaccg cctggatctt
      721 cgtcgccctc accgtgttgg ccgtatggtt cttcgcgcgc cgctgggcgg ctacacggac
      781 ggccaacctc gctgccgcca tcaccgcggt cgtgctcgcg ctgccgaatc ttgaggggtt
      841 caccgcgaat gccgagatct tcatgggcct tccaattgca ttcgccgcat tctggctcgt
      901 tcgccaggca caacttcgct ggtcgatgtg gcagctctgc ggggttggag tgctgattgg
      961 cctcgcgaca gtcctgaaac cctccggtat cgtaatggcg ttcacagcgc tagccttcat
     1021 cgccatggtg ggagaagatg tatcactcca acagcgattg catcgctgtg gcgccgtcct
     1081 cggtgggatg gctcttgtcg gcatcgtaac actcgcacac gggtggtatc tcggctggag
     1141 cgcttttatc tatgccaccg tgacgtatcg cctgaccctc cagagcagcg ccacggtgag
     1201 tgcgatgcgt catctcatct cccttggcag gctggcctgg acgatcgttg ccttgttatc
     1261 gctggtctat gtcatcttcg ccttccgaac ccatatctcc gtgtgcccgc cggcacatct
     1321 gcaaggggca gcaggttcct ggcaactcaa ggagacggtc cctgacagac cgggacatct
     1381 acgtctcttc atcacctcca ggcgatcact caacgacggg gaaactgcca gcctcatgat
     1441 ccagctttgg tccatcggtg ccattgcggg gatcgcgatg ggcggtgatt ggtggtcgca
     1501 ctacctcctc cagatcgccc cgcccttcgc gctctggctc gcctggaatc tcgaaaggat
     1561 tgttggcgtg ctccgccatt ggcagcgttg gctctttgtt gccattgcca gcgggctcct
     1621 gctgctgccc tatgaaatcc tcgtcgacgg cccagaggaa ttactcaatg accacccggg
     1681 ctatcccgcc caagccgagg tctcacgcta catccaggaa catagtgacc cacaggacag
     1741 gatctacgtc gccttcgacc aggcggcgat ctactacttg tcggaccgca aaccggccta
     1801 tcgtcatctc tacgatcagg agctccgagc gttgccaaat tcctacgccg atatcatcga
     1861 ggttatccgc ggcgatgacc ggccactcta catcgtcagc acgctgcacc cgggcccctt
     1921 ccccgatgac agccggacct tctggcggga agttgggcag tattacgaga tcgagacgac
     1981 gatcggtggc gtcccgatct accgggcgaa ggatgcaccc tgaacgggcg ccacgccggt
     2041 cagtcgaggc ggggcggtaa actgacagtg ccggctgtta tgactctgcg ctgctgattg
     2101 ccaggtcctt cgactccatc gcgcttccga tgttgacgcc gcttgtgatc agcccaatat
     2161 ggctaaaggc ttgcggaaaa ttcccgagga accggcccgt tcccggatcg atctcctcgg
     2221 gtaagagacc cagcctgttc gtccgcgcgc acatccggtc gtagcggtcc gcagcctcct
     2281 gcaaccgtcc ctgaagtatc aggttgtcga tcatccagaa actgcagagc acgaacgccc
     2341 cttcgtggcc ggatagccca tccggggatt tgtcgtgcag gtagcggtaa agcaatccgt
     2401 cgccggcgcc cagctggtct tcgatcgcgg cagcggtttt caccatcctg gaatgggttc
     2461 ccgggatagc tcggcgaagc gggaggctta gcagcgctgc gtccaggtgc ccaagttcct
     2521 gcccctggcc gtcttccgtg gtacggatcg tctgtgtcag gaactgttgc tcctcgtccc
     2581 aggcatcttc caggatcgtt cgctggatcc gctgggcctt gcgctcccat tcctcgacgt
     2641 ttccggacaa ctcgaaacgc cgcacgaggc gtatcccacg atccagcgca acatggcaga
     2701 gacccgcgga gtaagtctgg atgcgcccag gcgtgcgcac ctcccagatg ccagcgtcag
     2761 gagtgcgcca cacctcgccc gcgcgttcga caagctcatg aagacgaccc cagagcttgt
     2821 cgttcaccat ccccccgcgg tttgcccatt gatacgcgca atccaccagc tcaccataga
     2881 cgtcatgctg gatctgactc gaggctgcat ttccccagcg caccggtgca gatccacgat
     2941 agccagagag ctccgggtcg agtgactcgg ggatccgcgg ctccccgtac agcgtgtaca
     3001 tggggttgat ttgggtaccc tgcgccgttc caagcaccca ggtcagaaac tgccacgcct
     3061 cccggtcaag cccaatcctc cggagcgcat tgacggtgaa agccgcatcc cgaacccata
     3121 catagcggta atcccagttc cgctcgcctc cgatctgctc cgggagtgat gtcgttggcg
     3181 cggcgacaag cgcccctgtt ggcaggtaat ccagcagttt gatcgtgagt gcggagcgct
     3241 tgacatgttc ccgattggga ccttcatagg tgaaggcgcg catccacctc aaccaggttt
     3301 cggttgtatt ggtcaccgca gcgaccggtg catcgaccga ggatgccccg gtgccaccgt
     3361 tccagcggag gcagaacgtc accgattcgc ctcgcgttag tgtccattca ccgcttgtgc
     3421 cgctgaactc gcacgagctc tccactacca ggtccatccc tgggtagcag ggtgagctca
     3481 ggcgataccc attctcatgc ctctctgctt cgcatccgcc acggatagag atgcgtgcct
     3541 tgacggtaat ggttccttct acgatctcaa cacagcgaag caactcgccg acattaacgt
     3601 tgccctcaat attgagatcg gccccggcct gcgtaggcat caggtcggtg atgcggagga
     3661 cgccggattc cagcttcagt tccgttatca ggatcggcgt atccccgata tagcgatgcc
     3721 gcgcaccgat gatctggcca ccgtcgaccg tgaacgcccc accaagttca tggtcaagaa
     3781 tggagcaaaa gatgggttcg gaatcgaaac gcggagcaca cagccaggag attgtcccgt
     3841 cccggcccac aagggccgcc gtgctgcagt cgccgatgat cccgtaatcc tcgatcggca
     3901 ggtaaccgtc caggtacgct attggttcga aatcgctcgg gctctggtca aactgaagtg
     3961 tggtcatgca tacatctctc ctagcatgga cagagtcgac gaggaaagaa agccaagtct
     4021 gctagaggcg ccaggtgggt tcctgttcct tgcgcaaggt gtgagactca tcgacggcac
     4081 ccggtgtctc catctgctca tgcatgatgt aggcggcacg ggctaccaca tgcgatgcaa
     4141 tcggcgtcgt gacaacaacc aacataatca gcaatatcac gcggtacacc atcgtttcct
     4201 cggccaccac ggttgcgctc agcgcaagca caaccacccc aaggaagacg gacttgctgg
     4261 ctccgtgcag cttggtatag acatccggca tccgccagat gccgatgatg cccagcgtca
     4321 tgaccaaaag cgagacaatc agcatggcat cataaacata ggcacgcatg gctagaacaa
     4381 gcgcctttca gcgtgatacc gtgctgcggc aatcacggag ataaacgaga tcaaggaaag
     4441 caccacggct gcatccaggt agtacgcagt tccggtcgtc acggaataca caatgagcag
     4501 tgctacgagt acaagcgtca gcgtatccag cgagagcacc cgaaccaatc ccgattttgc
     4561 ccggacaatc atcagcacac aaatgcagaa gaggatgacc agccaaatca tggcgatgta
     4621 aaagacaacc tcatgcatgg ggctccccct cttctcgatc cgacccaggt aactccctcg
     4681 gcgcgtagtt tccatactcc caaagccgat agtacttctc cgcatcccgg cgcacggcgt
     4741 cgggattact cgcgtcgatg acatgcacaa gcatctctcg cgcgtcccaa tctacttcga
     4801 ccaggaacga tccgggcgac agggtcacga aatagccaac tggaccgacc ccatagggag
     4861 aatgatgccc aatcggcagc gttacaattc ccggcttttg caggggacgg atgcccaggg
     4921 tggtaaggct aacgacccag gttcccttca ggatgtcgat gagcaggtac cagaacagga
     4981 cgggtgcgta gatcaccagg tgcaaggcga acccggacgg cggcaagggt cgcggaatta
     5041 cctgcttccg gaatccccag agtagcccta tcgagatcgc gaatcccaat accaggttct
     5101 gccaggtaaa gtccgctgtc atcaggccaa acaggagtac cgagctggca aggattccga
     5161 tgacaaggat catggtacct gtgcctccgg gagcgacaac gcagcccgct cgaccaggtt
     5221 cagcagcgga tccggccaca cgccgatacc gaggatcaca agggccagca tcacaacgac
     5281 cacctgggca ccccatggac tcgaatcatg cgcttcgatc tccgcggtgc gccggcgata
     5341 caactggaac atgtagacaa aggagagtgc cccacccaga aagatcaatg caaccagcat
     5401 cgggcgatcc tccgcgatac cgacctggaa cagggccatc ttgccaagga acccggatgc
     5461 aggaggcaca ccggcgacgg agaaagcccc gattgcgaac gcagcgcccg ctaaccaccc
     5521 gcgcaagctt cccgacagga acagcaatgc tttgttggtg ctgttgagca cggtgtagag
     5581 gatggcggcg gtatagcccg ccggcccacc tattcccaag gcaatcagga tgtagccaac
     5641 ctggccgatt gccgaatacg ccagtacctc gttggtcgcg tgacgcgaga gcgcctggat
     5701 cgcaccatag atgatgcttg ccgttccgag tgcgatcaac acgggcgccg cctgccgcaa
     5761 ctccctcgga aagatgtcgg caccgaagcg aatcaggcca taggtcccga tatttgccag
     5821 cgcgccactg aggattgccg cgaccgacgg tcgcgtcccg gtgtacacgg ctggcagcca
     5881 gaaatgaaac gggaagagcc ccagtttgat cgagaatgcc acaaagatga tcgttgccga
     5941 gagaatcgct ggattctcct gtaccagcgg catctggtca cggatccggt acatgtccag
     6001 gaatccggtc gtgtggtaga tcgcggagat gccgatcagg aagaagacag acccgagcag
     6061 attcaccgta ataaagacga atgcggcccg gaactgacgc ggagactccc cgtagcttga
     6121 cagcacatag gccgagatca tcgagatctc gaaaaagaca tagaaattga aggcgtcgcc
     6181 agtgaagaca aggccggtga gccccaatcc catgaacagc acaactgcgg gatagatgcg
     6241 tgcgccaacc tcgttcacca cctcgtagac gagagcggcg aggagcactc cctgcgagag
     6301 caaggcaaag acgatgctca gcatgtcgac ccggagcgtg attccaacgc cgaaactcca
     6361 gccgccgacc gccaaactca tcggcccgtc ctgatagaca gtgatcccca gcgccatggt
     6421 ggagagaatg gcggacgcaa gcactgcgac tgacagccac gctgccggtt ttcgagtccc
     6481 atccaggaca gcgagcacaa atccaccaaa ccagaggacc gcgacaggaa gcgcaatcat
     6541 cggatggcct ccgcccgcgc acgccggtca tcaggagaag cctcgccgtc cgtatcgtcc
     6601 cgcacaccct cacgcaggtc ctcggtcatc ggctcgtccc gttctgcctg ctgttcttcc
     6661 gccgcagaaa gccgggaaag atcgacggag taatgcgata tgtagacgcg gtagacgagc
     6721 gcgagcgcga gtgcagccgt ggcaaagctg atcacaatcg cggtaagcac catcgcctgt
     6781 accaatggat ccgcagtcgg ctcctcgacc ggcaggatag gtgcggcacc acgccggaga
     6841 cctgaggcca tgatgaagag attggcggca tttccgataa ggatcatccc ggcgatcagc
     6901 cggatcaaat cccgtttgag catgaggtaa gcaccggccg cgaacatcac ggcgatggcc
     6961 agggaaatca ccaggatcac aacggcgcct cccctgattc gtcggccaac gaccctccat
     7021 gggcattcga gcgcaggtat cgtccacggt cgcggagttt gacctgtcgg acctcttccc
     7081 gggcaatcgc atgaagaccg cctacgcaga acccgtagac gatcaggaag acgccgatat
     7141 caaagaccac cggtgtgatg aactccagga caccgaagtg aactgcgtgc tcgttgatgc
     7201 cgggccaatg ggtgaaaagg tcttccccga agaggatcgg aacaaatgcg gtcagcaagg
     7261 cgaggcccag ccccacaaag ctgagaattg gcgcgatgcg gctgagagtg atctgctcca
     7321 actcctcggt gccgaaggcc aaaccctgca agagcactcc aagcgcggcg atgacgcctg
     7381 cagaaaaccc gtcgccgaca tcggcatagc ccttgagcat gattgcgata gcgattatcc
     7441 aagagggaag gagtatgatc cgcgcgccgc tctggagcat gccggcgtgc ttctggggat
     7501 tccgggccgg catcagcgca acctcccggt ccggagcagg ctgatgacgc caaggagcgt
     7561 cagcgcaata accgttgcct cgcccatagt atcgaagcca cggaagtctg ccaaaatcac
     7621 cgtcacgatg tctttcccat ggacagccgc agtgagcaca gtctgcgaat caatcacttc
     7681 agtcgacggt gaggggcggg aaagagcgcc ccataccacg aagaaggcca tactcgccgc
     7741 gaccatggcg agtatcaagt cacggcgcag gtagcgacgc tccgcccgaa cctcggtctc
     7801 gaatcgcaga attgggcgag gcataagcac aagcatgccg atcatgaaca tcgacagcac
     7861 ggtctccacc accagagcta cgagcgtgac atccggcgca cccatgaacg cgtagacacc
     7921 agcaaggctg aaacctacgg aagagaggct gatcgcgagg cgaaaatgat ccctcaccac
     7981 cacaaccaca agcgcgctcg cgacggaagc gacgagcatt gcaattatcg ggatgtcacc
     8041 gcgattgagg ctacccatgt cgaacgaacg aacggcattg ggtgtcacga tcagggcgag
     8101 cccaaccaag aggccggccg gaagcaggat cgtcgccaca cggctccgga ggtcgcgaac
     8161 ctcaatccgg tggacgctgt ccgataactg attgagcccc tgcattcccg cgtaatagag
     8221 ccgtgccggg ccaaagtagg aaccaaggtg cgccacgcca agtgccgccg gccaccagat
     8281 acgacgcgtc aggtaaatca ggagacccgt cacccaggtg gcgatcgcca tcacgttctc
     8341 atgccgcgca tccaggtgat aggcaatatg cagatgaatg ggtgcatcgg ccgaaacaga
     8401 cgctgcacgg tcaacgatcc cggtcgcagg tgcggtccaa acgccaccca cgagtgtcgc
     8461 gataccgagc accaccaccg gccagaccag gtacccggag acggcccggg tttccgctcg
     8521 caacgggccc atgaatgttt gccaccagaa tcgcaggata tatgcgaagg tcagggccgc
     8581 gccgagaacc gacatgaccg cgacctctgt cccgcgagca taggccgcag cgaaaaagag
     8641 ttcgtccttg aagaagccga tggccagagg cagcgcgatg atccccgctg cggcgaggcc
     8701 actgccggcc gcaaggagcg gcatccgtcg catcagaccg cctacgtccg ggaggttctt
     8761 cttgccggtc gcctcggtta ccgcgccagc agtcataaac aaggcactct tcgccaacgc
     8821 gtgggcgagt acatagaagg ttgcgccagc gacaccctct tccccgccca ttccaaacat
     8881 cgctacaacg tagccgtact gtgagatcgt ggagtaagca aggatctgct tcaagaccct
     8941 tcgcgtcagg gcgatgatgc ctccaactgc cattgaggca aaccctatga tcaggagtgc
     9001 attgagcatc cagccgtact gctcaaccaa cggatagaca cgcccgatca agaaaacgcc
     9061 tgcggcgacc attgccgccg aatgcagata ggcggagaca ggtgtgggcg ccgccatggc
     9121 gcgcggcagc cagaagtgca ttgggacctg ggcacttttc gcaagcgcac cgagcacaat
     9181 gagccccacc gcaatcccgg tatttcgtcc tccattgctt gcagagataa tctccggcaa
     9241 ggagaaggtt ccgtaaatct ggaagagcgc cagcgcacca aggagaacaa agatcgccgt
     9301 gatgccggtg accagcagcg ccatctgggc cgagctccgc gattcctcct cagcccggtc
     9361 gaatccaatg aggaagtagg aagcgattgc agtcaggtcc cagaagacga agatcaggat
     9421 gaggtcc
//
LOCUS       DASYWM010000102        11845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_172225, whole genome shotgun sequence.
ACCESSION   DASYWM010000102 DASYWM010000000
VERSION     DASYWM010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172225"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..1849)
                     /locus_tag="VGR29_04200"
     CDS             complement(296..1849)
                     /locus_tag="VGR29_04200"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV2072825.1"
                     /translation="MTIVLGRRPAIEQINPADVRKAEWVARFSTGARQQEIPTGPPPV
                     PMAEVRMHMPAVDASGRPQRLSRPDGLVGLYATRIDVQRAQRMTTGRKIRLAAGSRPC
                     TVVRETVTMGRGCPEHYQRLYPARRPALRRLRHGGEFMSPSSVSQSVRNRIDAMRPQF
                     GPGSWRELLAASFRYSRLSAPRRTVMASMAALIILAGAATSTLAQQRYEVQPGDTLES
                     IAAEFGVDPEGIYRSSYMPNGWSVEAGQVIVIPDQGQSPAEAAAMAAQLEGTSPWVMG
                     AHWVEYGDTLGSIGQAWGVAPDVLMSFNGIADPTQLVPGQRILIPYEREDQTVSTLAL
                     RTPDVAVPVANYSQSRNLSCEYAAAHAATTAFGAGIGEQTFISSVPQANNPHYGYRGN
                     IDGWWGNTDDYGVYAEALVPTLNANGYAGEVMYTEGDTAPLVAHLDAGHPVVVWLGFW
                     GDMREVLTDQDHYSVFAGMHVVTVYGYDTEGVYVMDPAKGTTQYYDLATFQQLWSVID
                     GMGLAVYAM"
     gene            2491..3699
                     /locus_tag="VGR29_04205"
     CDS             2491..3699
                     /locus_tag="VGR29_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV2072826.1"
                     /translation="MTIATHTSMHDLKDRFAGRTGVFGDSIWTGIFEHLRKHHDPVYF
                     GDGAPAQEVIPVDRMRDASRRAWDDAPDCLKYGDQQGYLPLRELVVERVAKRGIDASV
                     NNVLITGGSTQAIDLACRVFLEPGDAVIVERPTFLGAIEILQMFEVEIVDVEIDDEGM
                     RMDALEAALESHPNTKIIYTIPTFQNPSGTTLPLERRHRMIDLAREHNVAIFEDDPYG
                     ELHYGADVLPPLGALDGRVIHFGTFSKTIAPGIRTGFAIAPGEVLEKMLAIREVSDIS
                     NDRIMMRTVYHTAVDFLDEHVERACDLYRARRDAMLQALEEHMPEGVRWSKPDGGFFV
                     WITLPDAIGSNTLFDAGAENGVIVFPGEWFYPNREAHHNVRLSFSTVPEDRISIGIKR
                     LADTVRQLLG"
     gene            4017..4604
                     /locus_tag="VGR29_04210"
     CDS             4017..4604
                     /locus_tag="VGR29_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072827.1"
                     /translation="MGQLTLFDLDFDRLPLGPFDTPWWWLLIGISVILLVTLQGLETA
                     VEGTWPHQRRDNEYIPGVRGVKPAWAFAAILIIPGALAMIGIVAVIIWLDLDAPEGLN
                     LGGSLLALGWLLFLIFGLNLFGLGRVLGTLGAFGPIAIGIVLLVADGLLIVTLMDVLP
                     SWDVVEESLKRGLEAMLPFLDFEEEARSLRRFTRG"
     gene            4948..6345
                     /locus_tag="VGR29_04215"
     CDS             4948..6345
                     /locus_tag="VGR29_04215"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2072828.1"
                     /translation="MGGIFGSSGTVDLPAEHRTRRLRLPKNARNRRPQVKESSLLDDV
                     DFRMLWLSRLLSQTAQGALLYALLILVVDLSNRSFYNSLFVACSNIPSIAFGLPAGLV
                     VDSLSRKHLLVMLNGFRFVFMLFLVASEPSLTGVFAATLGIWIIHQFYSPSEASMLAD
                     LVPSDRYTSAQSLFNLALTISQALGLVLLAPLMLRLGGPELVFVMVGALWLVAGALTI
                     LLPSIPVEQSATRRPRRRGLIESLGDGWRFVRGDRPTLEAILDDVLVSVGMSSLVVII
                     PFYLERVLGTSKENTVFVFAPAALGLVIGLRMAPFLARVIGERYSASLSLAGFAFCIF
                     SLGFVEQAYRFLNDVLRIPLNQITDWLAISPLIFLAMLISIPAGITSSIVNVAARSIL
                     LQRSPSSVRGQVIATQGLIGNVFGLVPTLLAGIATDIFGVVPVAVALGVLILIAGGAA
                     HGAGRRPAPQALPAT"
     gene            complement(6390..7361)
                     /locus_tag="VGR29_04220"
     CDS             complement(6390..7361)
                     /locus_tag="VGR29_04220"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEV2072829.1"
                     /translation="MPDLAISYEDVVKAQERLDGVAHRTPTLTSMTADAMAGGQVFFK
                     CENMQRMGAFKFRGAYNALAQFTPEQKTSGVVAFSSGNHAQAISLSAKLLGVRAVLVM
                     PTDAPVSKVAATRGYGGEVILYDRYTENREEIGWRLADEQGLTLVPPYDHPDVMAGQG
                     TVAVELFEDVGPLDLLLVPLGGGGLLSGCAVAAHALSPGCRVIGVEPEAGNDGQRSFR
                     SGEIIHIETPKTIADGAQTLHLGNYTFPVIQQMVDDIVTVSDAELVEAMSFFASRMKL
                     IVEPTGSLAAAAVLQKKIDTRGKRIGVLLSGGNVDLARFADLVKGAS"
     gene            complement(7613..8443)
                     /locus_tag="VGR29_04225"
     CDS             complement(7613..8443)
                     /locus_tag="VGR29_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HEV2072830.1"
                     /translation="MTALVPRLIACFDVRDGLVTKGHAFQDNIDIAPVEELARFVYDE
                     QIDEVIVFDILASAERRGPDLDMVRRISRQLFVPLCVGGGIRSVDDMHEVLAVGAEKI
                     SIDSAAVRNPDLISQGAREFGSQAIVLSMQVKRVPPTKSIPSGYEIAIDGARTYTGLD
                     AVEWAARGAELGAGEICINSIDQDGTHSGFDLEITRMVANQVSVPVIASGGAGSASHI
                     AAAFREAGASAAIISSILYSPRIARNLGVRALKEQLTDLGIGMRPWMEIAASHDVAKG
                     "
     gene            complement(8440..9069)
                     /gene="hisH"
                     /locus_tag="VGR29_04230"
     CDS             complement(8440..9069)
                     /gene="hisH"
                     /locus_tag="VGR29_04230"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEV2072831.1"
                     /translation="MKPNVLVIDYRTGNSQSVSYALTNLGITNHVAQHPDQVGDVTHI
                     ILPGVGAAGTTMEYLASGEWLDFLNNRVLLQGLPFLGVCVGLQVLFDRSDEQNAKCLG
                     WLPGNVAAFDRQAVRVPHMGWNRVVRTGNHPFAAAISADSYFYFVNSYFAKPREMADV
                     AGTADYDGVFAAVIARKNIMATQFHIEKSGPSGLALLSRFTTLTPRELQ"
     gene            complement(9581..10624)
                     /locus_tag="VGR29_04235"
     CDS             complement(9581..10624)
                     /locus_tag="VGR29_04235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV2072832.1"
                     /translation="MQNLADILERHRRAFTVAGLGLSALVLVSCGGEDEEDPAEAVVE
                     EVVATEAPTEADEATMVATAAVGEADSPAASPTDVTIILPEGTADAAATPEISPAATP
                     DVPGGAPEIATPNASPVAAGLPSDRETPMASPIGATPVASPEVATPQAANGTPVGDLD
                     CWNEDPSMVAGYPQWSSAPEMAIDPSKTYIATIETNRGMIVAELYAEQAPTTVNNFVC
                     LAVQDYYEGVPFHRVIRGFMIQTGDPTGTGQGGPGYQIEDELPGDDLDYQEGVLAMAN
                     AGPNTNGSQFFINQANNSGRLEKNYTIFGRVIEGMDVVDAIAEVPVKAGPTGEPSVPA
                     AAMTVLSVEITEK"
     gene            complement(10931..>11845)
                     /locus_tag="VGR29_04240"
     CDS             complement(10931..>11845)
                     /locus_tag="VGR29_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072833.1"
                     /translation="LFWEMGRPNFQSLRVRRVRDDTERSTTLVTGAVDLIPGAPLLDI
                     PTLEAEPSVRLVGGPSNRLCLMHVNLHAPVLRDVRVRRLLSTAIDRQRLVQVATAGQA
                     VGTGLLIPEDSWAHGDVEETPQRAAEDVRAALAELGIRSDLPLRLITNNADATLANAA
                     VVLQEQLAYCGIALSVDLLEDDDFERAVSLGDYDLLAGYTRPWRDPHELVRPLLASDG
                     SGNYSGYASPEVDGLIRGATIVSDREIRQEHYTRLEEQVQVDVPVIVLFRPHYFDAMT
                     QRLDGYALLPPVTSRGLLPATLRPADNA"
BASE COUNT         2225 a         3772 c         3458 g         2390 t
ORIGIN      
        1 ggacttcgtg gcgcattcac cgtcttcgcc gtgctcctga tcgttgtcct gatcccggtg
       61 ttggggagcc ggtcgctcca tgaactcgat ggggagcctg atccctcgtt gggcgagaca
      121 gcgtaacggg ccccggaagc aggagactgt gtcaggggca agacgccgcg tttccggcac
      181 cacatgcgga ttgatccagg tttgacaaag gacaatacga acgtcccggg ggccccagct
      241 aagcccgtcg ggacgttcgt tttccgtgtg tgcaatggtt ggtggcccgt cgccgttaca
      301 tagcataaac ggcgaggccc attccgtcga tgaccgacca gagctgctgg aacgtcgcca
      361 agtcgtagta ctgcgtcgtc cccttggccg gatccatgac gtagacccct tcggtatcgt
      421 agccatagac ggtcacgacg tgcatgccgg cgaagacgga gtaatgatcc tggtcggtca
      481 ggacctcgcg catatcgccc cagaagccaa gccagacgac aaccgggtgc ccggcatcca
      541 gatgtgcaac aagcggagcg gtgtctcctt cggtgtacat gacctcgccc gcgtagccgt
      601 tagcattgag cgtcggcacg agcgcctcgg cgtagacacc atagtcatcg gtgttgcccc
      661 accaaccgtc gatattaccg cggtagccat agtgagggtt gtttgcctgc ggcacggagc
      721 tgatgaatgt ctgctcgcca atcccggcgc cgaatgcggt ggtcgcggca tgggctgccg
      781 catactcaca gctgaggttc cgcgactggg agtagttggc caccggcacg gcgacatccg
      841 gtgtgcggag cgcaagcgtc gatacggtct ggtcttcccg ctcgtagggg atcaggatgc
      901 gctgacctgg cacaagctgt gtgggatccg caatcccgtt gaagctcatc agcacatccg
      961 gggcgacgcc ccacgcctgg ccgatgctgc ccaaggtatc gccgtactcc acccagtgag
     1021 cgcccatcac ccagggactg gttccctcca gctgggccgc catcgccgcc gcctcggccg
     1081 gcgattggcc ctggtccgga atcacgatca cctggcctgc ttcaacggac cagccattgg
     1141 gcatatagct ggagcgatag ataccctcgg gatccacacc aaactcggca gcaatcgact
     1201 ccagcgtgtc gccgggctgc acttcatacc gctgctgcgc cagcgtcgaa gtcgccgctc
     1261 cggcaagaat gatcagcgca gccatcgaag ccataaccgt ccgccggggt gctgaaaggc
     1321 gcgagtacct gaacgacgcg gccagcagct cgcgccagga tccgggccca aactgcgggc
     1381 gcatcgcgtc gatgcggttg cggacggact gtgacactga cgacgggctc atgaactctc
     1441 ctccgtgacg caaccggcgc agtgctggcc gccgtgcggg atatagacgc tggtaatgct
     1501 cggggcaacc ccgccccatc gtgaccgttt cacgcaccac agtgcagggc cgcgaccccg
     1561 cggccaaccg gatcttgcga ccggtggtca tccgttgcgc gcgctgcaca tcgatcctcg
     1621 tggcatagag cccgacaagg ccatccggcc gcgacaatcg ctgaggccgc ccgctggcat
     1681 caacggctgg catatgcatg cggacctccg ccatcggaac tggaggtggg cccgtgggga
     1741 tctcctgttg ccgtgcaccc gttgagaagc gtgccaccca ctcggccttg cgcacgtcag
     1801 cagggttaat ctgctctatg gctggtcggc gtccgagcac aatcgtcatc gcggcaacct
     1861 ccacccccca agcaagcggc ggggatcgcg ctcgggactc tacgaggatt cagcgggctg
     1921 aatccgtcct ttattcaatc gccattcaca ccgatcaggt gtgtacgtac accgagatca
     1981 tagtggacgt gtgccaaatt gacaagggcc aaggttggca tggaacacca gcctccgggg
     2041 caagaccatg agaggttcaa ggatgcttcc gcccttcgcc caagtctcgg cgatcagcgt
     2101 cccttgcgat gtttcgattc tccccctatc atcaagagca tcatccgtca catcgtgatc
     2161 gtgatgggcg tctgcccggt ccgcgaacga ccggcaaggc catactccaa ttcctccatt
     2221 gaggcgatca tgacgactca aggcgccatg atggcgcgca tccacagcct ccatttcctc
     2281 cgatcaacag gacgcgtctg tgcgtacctg cctcccgcca tcgccgagcc ccctacctgc
     2341 gcctgatcgg ccgcttctcg ccgggtcgat gcaggcacaa catggtcgtt gcagcaccgg
     2401 cagagcccac tgcccacgac agccatatcc atcaccgctc gcgcacgtac cgcggctccg
     2461 tacagggctg cggcagggag tctgaacgcc atgactatcg ccacgcatac ctccatgcat
     2521 gatctcaagg accgcttcgc cggacgcaca ggcgtcttcg gcgactccat ctggaccggc
     2581 atcttcgagc atctccgcaa acaccatgac ccggtttact tcggcgatgg cgccccggcc
     2641 caggaggtga taccagtcga tcggatgcgc gatgcatccc ggcgtgcgtg ggatgacgcc
     2701 ccggactgcc tgaagtacgg agaccagcag ggatacctgc ccttgcggga actcgtagtg
     2761 gagcgcgtcg ctaagcgcgg catcgatgcg tccgtgaaca atgtccttat caccggcggc
     2821 tccacgcagg caatcgatct cgcgtgccgc gtcttcctcg agccagggga cgcggtcatc
     2881 gtcgagcgcc cgaccttcct cggtgcgatc gagatcctcc agatgttcga ggtcgagatc
     2941 gttgatgttg agatcgatga cgaaggcatg cgcatggacg cgctcgaggc cgctctcgaa
     3001 tctcacccga acacgaagat catctacacg atcccgacat tccagaatcc ttcaggaacg
     3061 acattgccgc tcgagcgccg gcatcggatg atcgacctcg cccgcgagca caacgtggcg
     3121 atcttcgagg acgatccgta cggcgagctg cactacggcg ccgacgtgct gccgccgctc
     3181 ggtgctctgg atggtcgggt gatccacttc ggcacattct cgaagacgat tgcgcctgga
     3241 atccgtaccg gcttcgcgat cgcaccgggg gaagtcctgg agaaaatgct tgccatacgc
     3301 gaggtctcgg acatcagcaa cgaccggatc atgatgcgaa ccgtttacca caccgcggtg
     3361 gattttcttg atgagcacgt tgagcgagca tgtgacctgt accgcgcccg acgcgacgcc
     3421 atgctgcagg cgctggagga gcatatgccg gagggggtga ggtggtcgaa gccggacggc
     3481 ggcttttttg tctggatcac gcttccggat gccattggct ccaataccct cttcgatgca
     3541 ggcgccgaga acggcgtgat cgtttttccg ggtgaatggt tctacccgaa ccgggaagcc
     3601 caccataatg tgcggctcag cttctccacc gttccggaag accgcatctc gatcggcatc
     3661 aagcgtctcg ccgacactgt gcgtcagcta ctcggctaac gctttcgagc gccttcatcc
     3721 gggggaagca tccgtgcctt cccccggttc cctctccctc ccctgctgcc ccgtaaagag
     3781 ccccctactc ggcaactcac cgtgatcaag atcaggaggg caacgggacg ctcccgatta
     3841 tgttggtcct gagcgaaagg cccatcaact gccacctcgc cttcgcgaca tccgttttcc
     3901 ggtatgcttg ccgtggcctt cgaggtgctc gccgttcacg tctggaccgg cactccctcc
     3961 ccggctcatt cccttcggcc ggatccgccc cgctcccggc tcactgacca atctgtatgg
     4021 gacagctaac cttgtttgat ctcgatttcg accgcttgcc gctcgggcca ttcgacacgc
     4081 catggtggtg gctactcatc ggtatctccg tcatcttgct ggtcacgctc caggggcttg
     4141 agaccgctgt tgagggcacg tggccacacc aacgccggga caacgagtac atccctggcg
     4201 ttcgtggcgt taagccggca tgggcgtttg ccgcgatcct cattattccc ggcgcgctcg
     4261 cgatgatcgg tatcgttgcg gttatcatct ggctggatct cgatgcacca gaggggctta
     4321 atctcggtgg ttcgctcctc gctctcggat ggcttctctt cctgatcttc gggctcaacc
     4381 tcttcggcct cggccgggta cttggcacgc ttggcgcatt tggaccgatc gccatcggca
     4441 tcgtgctcct cgtcgccgac ggtctcctga tcgtgaccct gatggatgtg ctgccctcct
     4501 gggacgttgt cgaagagtcg ctcaagcgtg ggctggaggc catgctgcca ttccttgatt
     4561 tcgaggagga ggcccgctcc ctgcgccgtt tcactcgagg ttgagggcat agccttgcat
     4621 ccacggtggt atatgggatg cttgaaggag gcgtcagcag cttgggtggc aggaatatac
     4681 ctgccgtggt tcgggtagtc atgtcatgga ccgggagttc gtgggcacat gagcgagggt
     4741 gaaacgcgac ggtcacagtc cgcagacgcc gccccccggc cggccccggg cacccgtcgc
     4801 cgggcgcccg gcaaaaccgt tccacggcgg cgcccctccg ttcatcccgc acgaaaaacc
     4861 ctcgtggctc catcgcttcc tccttcacgc gtcgataggg atgacgacga tcggcaggat
     4921 cgatcgccgg gtgtggagct ccgtccgatg gggggcatct ttggcagtag cggcacggtc
     4981 gacctgccag ccgaacatcg tacccgccgg ctccgtctcc cgaaaaatgc tcgcaaccgg
     5041 cgtccgcagg tcaaggaaag ctcactgctc gatgacgtgg atttccgcat gctgtggcta
     5101 tcacgccttc tgtcgcagac tgcacagggc gccttgctct acgcgctgct gattctcgtg
     5161 gtcgatctct cgaaccggtc gttctacaac tcgctctttg tcgcctgctc gaacattccg
     5221 tcaatcgcat tcggccttcc cgctggcctg gtggtcgact ccctgtcgcg gaaacacctg
     5281 ctggtgatgc tcaacggctt ccgtttcgtc tttatgctct tcctggtcgc gagtgaaccg
     5341 tcgctaaccg gtgtcttcgc ggcgacactt gggatctgga tcatccacca gttctactcc
     5401 ccgtcagaag cctcgatgct ggcggatctc gttccctctg atcgatacac ctcggcccag
     5461 tcccttttca accttgccct aaccatctcc caggcgcttg ggcttgtgct cctcgcgcct
     5521 ctgatgttgc gtcttggcgg tcccgagctt gtgttcgtga tggttggtgc actctggttg
     5581 gtggccggcg cgctgacgat ccttttgccg tcgatcccgg tggaacaatc ggcgacgagg
     5641 cgtccacgcc gccgtgggct catcgagtca cttggtgacg ggtggcgctt cgtgcggggc
     5701 gaccggccaa cgctggaggc gatccttgac gacgtgctgg tcagcgtcgg gatgagctcg
     5761 ctggttgtga tcattccgtt ctacctggag cgagtactgg gaacgtcgaa ggagaatacc
     5821 gtctttgtct tcgcgcccgc ggcgctcggc ctggtgattg gcctgcgcat ggcgccattt
     5881 cttgcgaggg tcatcggcga gcgttattcc gcatcgctgt cgctggccgg ctttgccttc
     5941 tgtatcttct cgctgggctt cgtcgagcag gcctatcgct tcctcaatga cgttctccgt
     6001 atcccgctga accagatcac ggactggctg gcgatctcac cgctcatttt cctggccatg
     6061 ctgatctcga tcccggccgg catcacatca tcgatcgtca atgtggcggc ccggtcgatc
     6121 ctgttgcagc gcagcccatc ctcggtgcgc ggtcaggtga tcgcgacgca agggctcatc
     6181 ggcaacgtct tcggtctggt acccaccttg ctggcgggca tcgcgacgga tatcttcggc
     6241 gtcgttccgg tggcggtcgc gttgggtgtg cttattctca tcgcgggggg tgccgcgcac
     6301 ggagctggcc gccgccccgc cccccaggca cttcccgcaa cgtaggtggg tctcgcctgg
     6361 tcagcaggga cgggtcagcg atcggtgcgt caggaagcac ctttcacaag gtcggcaaaa
     6421 cgtgcgagat cgacattgcc gccggagaga agaaccccga ttcgtttccc ccgggtatcg
     6481 atcttcttct gcagcacggc cgcggcagca agcgatcccg tgggctcaac gatgagcttc
     6541 atccgcgagg caaagaagga cattgcttcc accagctccg catcgctcac ggtgacaatg
     6601 tcgtccacca tttgctgaat gacggggaag gtgtagttgc caagatgcag cgtctgcgca
     6661 ccatccgcga tcgtcttcgg cgtctcgata tggatgatct cgccggagcg aaagcttcgc
     6721 tggccatcat tccctgcttc cggctcgaca ccgatcactc ggcaacccgg gctcaaggca
     6781 tgagcggcca cggcgcagcc tgacagcagg cctccaccac cgagcgggac aagcagcagg
     6841 tcgagaggcc cgacatcctc aaacaactcc acggcgacag tgccctggcc tgccatcaca
     6901 tccggatgat catagggcgg caccagcgtc aatccttgtt catccgccaa acgccagccg
     6961 atctcctccc ggttctctgt gtagcggtcg tagagaatga cctcgccacc gtagcctcgg
     7021 gtagcggcga cctttgagac cggcgcgtcg gtcggcatga cgagtaccgc ccgtaccccg
     7081 agcagcttcg cggagagcga gatggcctgc gcgtgattcc ccgacgagaa cgccacgacc
     7141 ccggaggttt tctgctccgg tgtaaattgg gcgagagcat tgtacgcacc ccggaacttg
     7201 aaggccccca tgcgctgcat gttctcgcac ttgaagaata cctgtcctcc cgccatcgca
     7261 tccgccgtca tggacgtcag cgtaggggta cgatgggcaa ctccatcgag ccgctcctgg
     7321 gccttgacaa catcttcgta ggagattgcg agatcaggca tcaactgctc ctgaggaaag
     7381 gaccgcaaat acgtaggatt tctttagaag acaggctcag cgatctccct tgttacaacg
     7441 gcgacatcga gcttcgtgaa aagggtaagt caccggccgt tttctgcgcc gaggcagaaa
     7501 cgaggttacg ccaagccatc gaagactatg gaggagaacc ccgctacgct gtcatccatg
     7561 gcagcaggtg ctccagcgcc atatttgtgc gtccggcgga tctgagccgt ttttagccct
     7621 tggcgacatc atggctcgct gcgatctcca tccagggcct catgccaatg cccaggtcag
     7681 tcagctgttc tttaagtgct cggaccccta aattccgggc tattctcggc gagtaaagta
     7741 tcgagctaat gattgccgcc gacgcaccag cctcccggaa ggcagcggcg atgtgagagg
     7801 cgctgccagc tccccctgag gcgattaccg gaaccgatac ctggtttgct accatccggg
     7861 taatttccag atcgaagcca gaatgcgtac cgtcctggtc gatgctgttt atacagattt
     7921 ctccagctcc cagctcagcc ccgcgagccg cccactccac ggcgtcgaga ccggtgtatg
     7981 tgcgagcgcc atctatggcg atctcgtatc cgctggggat cgatttcgtc gggggaacac
     8041 gcttgacttg catggacagg acgatggctt gcgaaccaaa ctccctggcc ccctgggaga
     8101 taaggtcggg gttgcggaca gctgcagaat cgatcgagat tttctccgct ccaacggcca
     8161 ggacttcgtg catgtcgtca acagacctga tgccaccacc gacgcacaga ggaacgaaga
     8221 gctgtctgga gattctgcga accatgtcca gatcagggcc acgccgctcg gcgctggcga
     8281 gaatgtcgaa gacaataact tcatcgatct gctcgtcgta gacaaaacgt gccagctcct
     8341 cgacaggcgc gatgtcgata ttgtcctgga aggcgtgtcc tttcgtaacc aaaccgtctc
     8401 gtacgtcaaa gcatgcgatc aagcgcggta cgagagctgt cattgcagtt cccttggggt
     8461 gagggtcgta aagcgactga ggagcgcaag accgctgggg cccgactttt cgatatggaa
     8521 ctgggtagcc atgatgtttt tgcgggcgat cactgcggcg aacactccat catagtcagc
     8581 agttcccgcc acatcagcca tttcgcgtgg cttggcaaag taggaattga caaaatagaa
     8641 gtatgaatcg gcagagatcg cggcggcaaa gggatgattg ccagtgcgga caacgcggtt
     8701 ccagcccata tgaggcacgc gtactgcctg ccgatcaaag gcggccacgt tgcctggtag
     8761 ccagccaagg cattttgcat tctgctcatc acttcggtcg aagagcacct gcaggcccac
     8821 acagacgccc aggaaaggca gtccttgcag gagaacgcga ttgttaagga aatcgagcca
     8881 ctcgccggag gcaagatact ccatggttgt tcctgctgca cccacaccag ggaggatgat
     8941 gtgcgtgaca tcgcccacct ggtcagggtg ctgggcaaca tggttcgtaa ttcccaggtt
     9001 ggtcagagcg tacgagacgc tttgagaatt tcctgtgcgg tagtcgataa cgagcacatt
     9061 gggcttcatt ctgcctcact tcaagagttc aggccttgca acgggccgga cgtccccatt
     9121 tgccagggtt cgatgcaaca ctgatcgctt ctttagattc cagagcggcg accagaaatc
     9181 gtgcccgctg atgacacccg gatcgccgct cccggcaaac cttttgaaga acgagcctgt
     9241 atcactttca gtgatacagg ctctagccga cagacaattg ccgccagaaa cggtgtaccg
     9301 gcgtcgattg tatgacagga tatcaatcta cgccgaaagg aggctgcgac agttcccagg
     9361 cgaccggcgc atccttctca atgccgacaa cgtacagtct tgtgacctac agcatcggtg
     9421 ggcgcgagcc ctgacggggg cacgcgaggg actggcccga gggcaagtga tcctgttcga
     9481 cgggttccac cgggttcatg catccccaca gcaagaatac ccggaccctg acaaacaggg
     9541 cccaggcatt gccacaccgc tcccgtgtga cgagcgaaag ctacttctct gttatctcga
     9601 cggacaggac cgtcatcgcc gccgccggaa ctgaaggctc tccagtcgga ccggccttca
     9661 ctggcacctc ggcgatcgca tcgacgacat ccataccttc gatgaccctg ccgaagatcg
     9721 tgtagttctt ctccagccga ccggagttgt tcgcctggtt gatgaagaat tggcttccat
     9781 tcgtattcgg gccagcgttt gccatcgcca gcacgccctc ctggtagtcg agatcgtcgc
     9841 cgggcagctc gtcctcaatc tggtatccag gtccaccctg gccggttcca gttggatcgc
     9901 cagtctggat catgaacccg cggatcaccc ggtggaacgg aaccccttcg tagtaatcct
     9961 gcacggcaag gcagacgaag ttattgaccg tcgttggggc ctgttcagca taaagctcgg
    10021 cgacgatcat accgcggttg gtctcgatcg tcgcgatata cgtcttcgac ggatcgatcg
    10081 ccatctctgg cgcactggac cactgcggat acccagcgac catactggga tcttcgttcc
    10141 agcaatcaag gtctccaaca ggagtgccgt tcgccgcctg gggtgttgca acctccggtg
    10201 aagcaaccgg cgtcgctccg atcggcgatg ccatcggtgt ctcgcgatct gagggaagtc
    10261 cagccgctac ggggctggcg ttcggtgtcg caatttcagg cgcaccgcct ggaacatcgg
    10321 gcgttgccgc cggtgagatt tcaggggtag cagctgcatc cgcggtgcct tccggcagga
    10381 taattgtcac gtcggtaggt gacgcagcag gtgaatcggc ctctccgaca gcagcggtcg
    10441 caaccatcgt tgcctcgtcc gcttcggtcg gagcttcagt ggcgacaacc tcctcgacca
    10501 ccgcctccgc tgggtcctct tcgtcttctc caccgcagct tacgagcacc agcgccgaga
    10561 gtcccaaccc ggcaaccgtg aatgcgcgcc ggtgtcgttc taggatatcc gccagattct
    10621 gcacgttgtt tcttccttct cctgggtgct acccaaaata ccaatacctg tcggacccag
    10681 cctgtcaatc tggccgtagc cgattcttca tggtggccaa agctgtgtgc aattcgacct
    10741 ttagcgggtc ttcagagaga gcggtctacc cggtgcccac gcatctcccg tcacgaatca
    10801 ggatccgatc atgaccggta ccaaggcctg aaacatggtt gatccccgac gggagaatca
    10861 caccactcac catatccttc ggagtatcga agacgctcct cagcgaaatg tcccggaaaa
    10921 gaatgccaca tcaggcgttg tccgcgggtc gcagcgtcgc tggcagcaac ccgcgggagg
    10981 tcaccggggg caggagcgcg tagccatcca gacgctgggt catcgcatcg aaatagtggg
    11041 gtcgaaacag cacgatcacc ggcacatcaa cctgcacctg ttcctccagg cgagtgtagt
    11101 gctcctgccg aatctcccga tcggacacga tggttgcccc tcgaatgagt ccatcaacct
    11161 ctgggctcgc gtacccggag taattacctg acccatctga tgccagcaat ggccggacca
    11221 gctcgtgcgg atcgcgccac ggtcgcgtat acccggctag gagatcgtaa tcacccaggc
    11281 tgaccgccct ctcgaaatcg tcatcctcca gcaggtcgac ggacagggcg atgccgcaat
    11341 aagctaactg ctcctgcagg accactgccg cgttggccag cgtcgcgtcg gcgttattgg
    11401 tgatcaggcg caacggcaaa tcggatcgaa tacctagttc ggccagtgca gcacgtacat
    11461 cctcggctgc acgctgcggc gtctcctcca catcaccatg cgcccacgag tcctcgggaa
    11521 tgagcaggcc ggtccctact gcctgccccg ccgtcgcaac ctgcacaagc cgctgccggt
    11581 cgattgcagt tgacagcaag cgccggacgc gaacatccct cagcacgggc gcgtggagat
    11641 tcacatgcat gaggcagagc cgattggagg gtcctccgac taaccgaacc gatggctctg
    11701 cctccagggt cgggatatcc aggagcggtg cgccggggat gagatcgacg gcacccgtca
    11761 ccagggtcgt ggaccgttcc gtatcgtccc gaacccttcg gacacggagg gactggaagt
    11821 tcggcctgcc catctcccag aagag
//
LOCUS       DASYWM010000103         4509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_153551, whole genome shotgun sequence.
ACCESSION   DASYWM010000103 DASYWM010000000
VERSION     DASYWM010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153551"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VGR29_04245"
     CDS             complement(<1..402)
                     /locus_tag="VGR29_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072834.1"
                     /translation="MANRYTGGASVVSMDGYDVGTVERVDDDYLVVRLRNSPRSMRVP
                     FDVIDNEASTNDRIVIQGAVGDLEASRTNPRITEPGDGVTLPLVGEEAIAHVREVSRG
                     RVVVDKHVEMVPHEARIDVGTDRVDVERVAVN"
     gene            complement(467..1387)
                     /locus_tag="VGR29_04250"
     CDS             complement(467..1387)
                     /locus_tag="VGR29_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEV2072835.1"
                     /translation="MTEAGLSRIYSIGEAVAELQRVYPDVTHSSLRFLERDGFVEPER
                     TPGGHRKYRRKDIDRVRMIKDWQAERLTLEEIRERIQRLESMPEPENIWPRFLDLALK
                     GQFQQAAAIILGADEVGMPLVTIFQDVLTPALIEVGRRWATDELKVGEEHEVTELCRD
                     LISELTMRHMGRTAEGPIVLVAAVEAEMHDLGLRMVCGLLRQRGAHVHFLGSNVPTPF
                     LLESVLLRRPDIVLLSVTLEEHRPSLERAIDALRRELPGGVSPVVVIGGQGGVNAADA
                     FTGPETNVMEPGGLSTAVDEIISYGTRVDQ"
     gene            1785..2363
                     /locus_tag="VGR29_04255"
     CDS             1785..2363
                     /locus_tag="VGR29_04255"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEV2072836.1"
                     /translation="MTDQEKKVNTTISDWLGDIVALESHVEEAMDHQLKLEAPSDSLN
                     QAIKHFHDSVRASKRRAIAYQEQYGSTTGNPIIKAGSELLGKAAGIIDKMRNDSVSKA
                     LRDDYTAYNHVAIAYTMLHTTAMALEDHATQQFAEEGLRTYASMVQELNHVIPEAVLL
                     DLKHNDEVPVLDPTIVDRCRATIDSVWKSTQA"
     gene            2541..2693
                     /locus_tag="VGR29_04260"
     CDS             2541..2693
                     /locus_tag="VGR29_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072837.1"
                     /translation="MKRMFGPREFRGGRVQVYGCSPGCLLISLLVSVVASIILTLLLN
                     LLPRLF"
     gene            complement(2770..3654)
                     /gene="proC"
                     /locus_tag="VGR29_04265"
     CDS             complement(2770..3654)
                     /gene="proC"
                     /locus_tag="VGR29_04265"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV2072838.1"
                     /translation="MTSDTAGAAVGTLSSSRLAIIGAGVMAESMIAGLLAGDMIAPDR
                     IVASHPRVERRDQLAAKFGIRAVESNAEAARDADLILLTIKPQVLSGVMRQMKGSLRE
                     GQVVISILAGASIRALRTGLDHDAIVRVMPNTPAQIGEGMAVWCCTGAVDEIARDRVR
                     AALGALGDELQVDSENYVDMATALSGTGPTYVFLMMEALIDAGVHMGFPRRIAEQIVL
                     QTVGGSVAYARSSGKHLAELRNMVTSPGGTSAAAIYQMEKGGLRTILSKSVHAAYQRT
                     QTLAAEQEQKLGEGMKPG"
     gene            3884..>4509
                     /locus_tag="VGR29_04270"
     CDS             3884..>4509
                     /locus_tag="VGR29_04270"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2072839.1"
                     /translation="MRARSIAAAPDAISNLARRFLPVAIFFVIAFAVDMLSGQRYSSL
                     WGSAAIVGSVVHLFPAARPTLAAIGAYVALWVSFNLVRAIGDDAGLAIAGPGTVSRLE
                     GSLFGGALPSAWLQTSFFDSTQVQVRDVALSLVHGSFFIVPFLVAILVWWRFRELFHP
                     YAMATAVTFALGLVGFLLLPTAPPWMSNPDDVTRVTRHILVETAGLSAG"
BASE COUNT          890 a         1448 c         1231 g          940 t
ORIGIN      
        1 gtttactgcc acgcgctcga catcgacccg gtccgtgccg acatcgatcc tggcctcatg
       61 gggcaccatc tcgacatgct tgtcgacaac cactcgaccg cggctgacct cacggacatg
      121 ggcgatggcc tcctcgccca ccaggggaag ggttaccccg tcacccggct ccgtaatacg
      181 aggattcgtc cggctcgctt cgaggtcgcc cacggcaccc tggatcacga tacgatcatt
      241 ggtgctcgcc tcattgtcga taacatcaaa ggggacccgc atggaacgag ggctgttccg
      301 cagacgcacg acgaggtagt catcgtccac gcgttccaca gtgcctacat catatccatc
      361 catacttacc acgctggcac cgccagtgta tcgattcgcc atctctatgg tcctttcgtg
      421 tggaaaccag tctgatatgt ggcgtctgat gcaagcatcg tgccagtcat tggtcaacac
      481 gcgtaccata gctgatgatt tcatccactg cggttgacag cccgcctggc tccattacgt
      541 ttgtctccgg gcccgtgaag gcgtcggcgg cgttcactcc gccctgcccg ccaataacca
      601 ccacagggga cacacctccg ggcagctccc gacggagtgc gtcaatagct cgctccagcg
      661 acggccggtg ctcttccagt gtgacagaca ggaggacgat gtctggtcgt cgcaagagca
      721 ccgactccag cagaaagggt gttggcacgt tggaccccag aaaatgcacg tgggcgccgc
      781 gttgccgaag cagcccgcaa accatccgga gtccgagatc gtgcatctca gcttctacgg
      841 cggcaacaag gacaattggt ccctcagccg tccgccccat atggcgcatt gtcagctcgg
      901 aaatcaggtc ccggcagagc tcggtaacct cgtgctcctc cccgaccttg agctcatccg
      961 tggcccaccg ccggcccacc tcaatgagtg ccggcgtcag gacatcctga aagatggtca
     1021 ccaacggcat gccgacctca tccgcaccga gaataatcgc cgccgcctgc tgaaactggc
     1081 ccttgagtgc aaggtcgagg aaccgcggcc agatattctc tggctccggc atcgactcca
     1141 gccgctggat cctttcccgg atttcttcca gcgtaagccg ttctgcctgc cagtccttaa
     1201 tcatccgaac gcggtcaata tcctttcggc gatatttccg atggcctccc ggagtgcgct
     1261 ccggttcgac aaacccgtcg cgctccagaa acctgagtga ggaatgagtg acgtcagggt
     1321 agacgcgctg cagttccgcg acagcttcgc cgattgaata gatccgcgac agtccggctt
     1381 cagtcatcgt gccacctttg ctttccttat gtacatgatg tacatgcatg cgttctcgtg
     1441 ttcaatattg atttcatcac acacctgttc ccggcagggt acaggaccgc gatattgaca
     1501 actataaacc taaagtgcac atttatgtat tcgtatcgcc acaccccgcg ggcacagttg
     1561 atttgaaccc gggtttctat tgaatgggct cgggacatgg caccggacgg gcagggcttg
     1621 aatgggcctc cagcgtggac ggcgcgccac cgatagcaag gggcaggagc gatcccatag
     1681 gcaagccgag caggctctat cggcagtggc agcgctcttg cgagtagtcc gagcggaaat
     1741 ccgttcaagg ttctttccca caacgagaaa gcgagaaaaa acacatgacc gatcaagaga
     1801 agaaagtcaa caccacaatc tccgattggc tcggcgatat cgttgcgctc gagtcgcatg
     1861 tcgaagaggc gatggaccac cagttgaagc ttgaggcacc aagcgactcg ctgaaccagg
     1921 ccatcaagca tttccacgat tccgtgcggg cgagcaagcg tcgagccatt gcctatcagg
     1981 agcagtatgg ctcaactacc ggcaatccga tcatcaaggc tggctcagag ctgctgggca
     2041 aggccgccgg tatcatcgac aagatgcgga acgattccgt ctccaaggcg ctccgcgacg
     2101 attacaccgc ctacaaccat gtcgcgatcg cctacacaat gctccatacc actgctatgg
     2161 cgctggagga ccatgccacg cagcagtttg ccgaagaggg gttgcgcacc tacgccagca
     2221 tggtacagga gctcaatcac gtcatcccgg aagcggtgct tctcgacctg aagcacaacg
     2281 atgaggttcc ggtgctcgat ccgaccatcg tcgatcgctg ccgggccacc atcgatagcg
     2341 tctggaagtc aacccaggct tagtgtcgac atcacgtgaa cgcgggaaaa cgaggaggct
     2401 cttccagcct cctcgtttct agttaaaggt tcagagttat ccgtggcatt ggccctgcct
     2461 tgctggcagt caggggaggc ggcgatgcct gccagaaaag ttcgcgccgg acacgactgg
     2521 aatggggagg aacagcgacc atgaaacgaa tgtttgggcc gcgagagttt agaggcggac
     2581 gtgtgcaggt ctacggttgc tcaccgggat gtctcctgat ctcactgctt gtctcagtgg
     2641 tggcatcgat catcctcacc ctcctgctca acctgctgcc gcgcctattc tgaacctaca
     2701 gaccagtgcc ataccggaag gtatctactc caaaggcgaa gccgctctat ccctctcccg
     2761 caattccact cagccaggtt tcatgccctc tcccaacttc tgctcctgct cagccgcgag
     2821 tgtttgtgtc cgttggtatg ccgcatggac cgacttcgag aggatggtcc gcagcccacc
     2881 tttctccatc tggtagatcg ccgccgccga ggtgccacct ggcgaggtca ccatgtttcg
     2941 cagctctgcc aggtgcttcc ccgagcttct tgcatacgcc acggacccgc ccacggtctg
     3001 cagcacgatc tgctcggcga tgcgtcgcgg gaaacccata tgcacgccag cgtcgatcag
     3061 ggcctccatc atcaggaaga cgtacgttgg cccggtacca gaaagcgcag ttgccatatc
     3121 gacatagttc tcggaatcta cctgcagttc atcaccgagg gctccgagtg ccgcgcggac
     3181 gcgatcccga gcgatctcat ctaccgcgcc ggtgcagcac cagacagcca tcccctcgcc
     3241 gatctgggcc ggcgtattgg gcatcaccct gacgatggca tcgtgatcca gtcctgtgcg
     3301 aagggcccgg attgatgcac cggcgaggat cgatatcacc acttgcccct cgcgcaggct
     3361 gcctttcatc tgccgcatca cccctgagag aacctgcggc ttgatcgtca gcaagatcaa
     3421 atcggcatcc cgcgcggctt ccgcgtttga ctccacagcc ctgatgccga acttcgccgc
     3481 cagttgatcg cgccgctcca cccgtggatg gcttgcgacg atacggtccg gcgcgatcat
     3541 atccccggcg agaagtccgg cgatcatcga ctctgccatc acgccggctc cgattatcgc
     3601 cagccgggaa gaggagagcg tcccgaccgc ggctcccgct gtgtccgatg tcataaccgc
     3661 ctcaccttac cctttcgatg cctcacaggg agccccaacc tcattccctt gtcttctatg
     3721 tagcgtatgt cgggtgacca caatcctcaa ttatgcacgc gacctcatcc atttccggct
     3781 ttcggcacgg tctttgcgtg caacttgccg agagcaccgg ctccaccaaa tcattgaagg
     3841 actcagcccc tggaggattg cgagcgtgaa gcaggaggga accatgcggg ctcgcagcat
     3901 cgcagccgct cccgatgcga tatcgaacct ggcgcgacgg tttctgcccg tcgcgatttt
     3961 tttcgtcatt gcctttgcag tcgatatgct ctccgggcag cgatacagtt ccctctgggg
     4021 aagcgctgca attgtcggct cagtagtcca tctcttcccg gcggcacgac caaccttggc
     4081 cgccatcggc gcgtatgtgg cactctgggt cagcttcaat ttggtccggg ccatcggcga
     4141 cgatgcagga ttggcaattg ccggacctgg cactgtatcc cggttggagg gatcgctttt
     4201 tggcggagcc ctgccgtcgg cgtggctcca aacttcgttc ttcgattcca cccaagtgca
     4261 ggtgcgtgat gtggccctgt cgctcgtaca tggctcgttc ttcatcgtgc ccttcctggt
     4321 tgcgattctg gtgtggtggc ggttccggga gcttttccat ccctatgcca tggccaccgc
     4381 cgtcacgttc gcgctcggcc tggtcggctt cctgcttttg ccgacagccc caccgtggat
     4441 gtccaatccg gacgatgtga cacgagtcac gcgccacatc cttgtcgaaa cggcaggact
     4501 atccgcagg
//
LOCUS       DASYWM010000104        28642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_191175, whole genome shotgun sequence.
ACCESSION   DASYWM010000104 DASYWM010000000
VERSION     DASYWM010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 28642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28642
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191175"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VGR29_04275"
     CDS             <1..398
                     /locus_tag="VGR29_04275"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV2072840.1"
                     /translation="STVVFQTYTPEHYAIRAASRHDYQAFYADEITFRQDFRFPPFLR
                     LARFMFRHDKEEGAALEAGLLARELARHARVNDIEMELLGPTPAFVAKIRNQYQWQIV
                     LRSRQMDRMLEGMPMRPGWVVDIDPESML"
     gene            408..989
                     /gene="def"
                     /locus_tag="VGR29_04280"
     CDS             408..989
                     /gene="def"
                     /locus_tag="VGR29_04280"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV2072841.1"
                     /translation="MARGTMEIRRYGVDGMAILDIVLEGDPVLRKKAVKVPRADDGIR
                     KLAANMYETMLEAPGVGLAAPQIGKSIRLIVVHVPEDEDDETPEVSLKLLNPEIIKAF
                     GTQVGAEGCLSIPGWAGNVERAMNITVKATDMNDKPVRIRAQGYLAVVLQHEIDHLDG
                     ILFPDRIENTKEDLWPVRESEEELVEDEGIPVA"
     gene            complement(1407..2120)
                     /gene="gmk"
                     /locus_tag="VGR29_04285"
     CDS             complement(1407..2120)
                     /gene="gmk"
                     /locus_tag="VGR29_04285"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV2072842.1"
                     /translation="MAQNTPATSAMSETFPSDHDARLGQLYAEGNALITGIRKGNKPR
                     VFIISGPSGVGKDSVIESLRNVFTDAQYVVTATTRPMRPGEIDGVHYIFLSKEEFLDR
                     IEKNDFIENALVYDNHYGVPRSPIVRGLEQGHDVIIKVDVKGAATLRRLIPNTISLFL
                     CPESMPALLNRLRDRKTDDPEVLMKRFTTATAELEEAQNFDYVVFNEDGKLDGAVEQI
                     CSIVSVEHRKVHQPEIEIP"
     gene            2206..4059
                     /locus_tag="VGR29_04290"
     CDS             2206..4059
                     /locus_tag="VGR29_04290"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HEV2072843.1"
                     /translation="MFDVLTAGAVVDELRRSVLDGRIQRLGLVDAFTIGAEIYAQGRR
                     RHLIASASPQNPRIHLTRSMPSTDPNLITPFGLQLRKYVRGGFLIGIEQPPLERVIRL
                     SIAKRMASLNSSGAEEGPDDEEEGGEDDIWSGQDVSRLELVIEIMGRHSNLILVDDQG
                     LVRESAKRVTSSMSRVRPILPKVPYDLPPAPDKPDPRRVTSPSMEQFLVNAKPDAKLA
                     DLLVRGYRAISPQIAREAAFRAAGDPRVKVAALGQEDAREIAQVVRGIFQPLLTGEWE
                     PHLYERDHLPIGYGAVPIRYLAGEAEDVPLESISEAIERAQDATAEVTPKDHAQRRAR
                     LLQAIDAAIKSVDTRIRSLQQQHERSRDTERLRRWGETIYAYLWQIQLGDAELIVETE
                     AGEEKIPLDPAKDPKEVAQEYFEEYRKAQKAGNTLPERIEAAENERRYLQQLRTQSQQ
                     AEGFAAIEALRQEYGEHSGGRHAVEEKPGHKASRKQSARKVAPLTDDDGNLIYIGRSG
                     RENEQVTFEIGGSDDLWLHARGVPGSHVILRMRAPSQEPDNSALETAASLAAYYSGSR
                     ESGTVEVDVTARRHVRKIKGGGPGMVTYRNERTIAVRPSDESQLRKAGQIE"
     gene            complement(4241..5107)
                     /gene="tatC"
                     /locus_tag="VGR29_04295"
     CDS             complement(4241..5107)
                     /gene="tatC"
                     /locus_tag="VGR29_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEV2072844.1"
                     /translation="MARTLKSLPFGKIRVPRLPEITDEPDVFDEMTLQEHLEELRDRI
                     MKMVIAIVPTFLFGFFIAGRILSDISNKANAVDGLDVKSPTETLTLTFKIALYVAIAI
                     CMPVIVYQFVAFLAPGMTRKEKRVLFSALPFTTILFAIGVYYGYFIAAPRALYFLSNW
                     NTNAFDWNPDGNETVSFFMTLMIGLGLAFQLPVIMFIVAKIGVITPRQMRKWRKYAFM
                     LLLVAAAIITPSTDPYNMAIVAIPLIILYEFGIIISSIFAKTGLRGENFSSDDAQEVE
                     LVPSRIEKADDI"
     gene            complement(5387..5626)
                     /gene="acpP"
                     /locus_tag="VGR29_04300"
     CDS             complement(5387..5626)
                     /gene="acpP"
                     /locus_tag="VGR29_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV2072845.1"
                     /translation="MATTLERVQNIVTDRLGVDAEQVVPEAEFIGDLNADSLDLVEVI
                     MAMEQEFDLEIKDEDAEKIRTVGDAVNYIEQSSAS"
     gene            complement(5904..6395)
                     /gene="nusB"
                     /locus_tag="VGR29_04305"
     CDS             complement(5904..6395)
                     /gene="nusB"
                     /locus_tag="VGR29_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV2072846.1"
                     /translation="MSGVRHQARVLAMQALYERDLTEHDLHEILERMRIGDDVDLPAS
                     ATETTDEDETQLEDIPQSVADRATAIVHGILEHQDGIDPLIEKAAPQFPIPQLAAIDR
                     NVLRLAIYELLYAPDVPYKVVINEAVEIAKRFGGPNSGRFVNGVLGTISKDLPAKRKH
                     SSR"
     gene            complement(6582..7019)
                     /locus_tag="VGR29_04310"
     CDS             complement(6582..7019)
                     /locus_tag="VGR29_04310"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV2072847.1"
                     /translation="MTDHQAPDKLIAPSRDKAEVNATFQADDATEVRGTVRIAPAVLI
                     QLIEVTVSDISGVLELKPGKRSKNDETPPLGGKSFDDGKVRVCVDGDQIDADISIAVR
                     RGTNISTLADEIRRKVGVAAGRMLGMTVRSVNIFIDDIVDPAA"
     gene            complement(7025..8434)
                     /gene="accC"
                     /locus_tag="VGR29_04315"
     CDS             complement(7025..8434)
                     /gene="accC"
                     /locus_tag="VGR29_04315"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV2072848.1"
                     /translation="MFKKILIANRGEIALRILRACRDLRIPAVVAYSDVDHDSLPVRL
                     ADEAVCIGPAPAARSYNNIPAVISAALVTGCDAIHPGYGFLAENAYLAEICQQVGLTF
                     IGPPPDVIEKMGNKAEARKVMKAAGVPLLPGTQGIVPTLTHARQAAREIGYPVVIKAV
                     AGGGGRGMRVATNDAELTRGYPVAQSEAESAFGNGDVYMERYLQRPRHVEVQVIGDQH
                     GNVVAIGERDCSLQRRHQKVVEEAPAPHLPDKVRQNLLKTAEKGARAANYWSAGTLEF
                     LLDTDGHFYFMEMNTRIQVEHPITEEVTSLDLVAWQIRVAAGERLTIHPEQLKPRGHS
                     IECRITAEDAANEFRPSVGTVETYVAPGGPGVRMDSHLFSGYSIPPNYDSLLGKVITW
                     GTTREQAVARMERALRETVLTGVTHTIPFHREILADAAFQRGEVHTGFIPELMSRLGT
                     RLKDAAEEDAMPQRLPIGR"
     gene            complement(8574..9329)
                     /gene="fabG"
                     /locus_tag="VGR29_04320"
     CDS             complement(8574..9329)
                     /gene="fabG"
                     /locus_tag="VGR29_04320"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV2072849.1"
                     /translation="MTEALRSPVAVVTGGTRGIGLAIARRLAKDGFDLVLTYRGDTEA
                     AAATEKELAGSGRSVRVITADVSTQEGAQLTVDTAVKELGSIDVLVNNAGITRDNLIM
                     RMTEEDWDAVLTTNLKGAFLTSKAAVRPMLRQRSGRIINLTSVVGLVGNAGQANYAAA
                     KAGLVGLTKSLAKEVGSRGITVNAVAPGFIDTRLTDVLPEDLKQNLLANTPLGRFGSP
                     EDVANAVAFLASPDAGFITGNTLSVDGGLFMAS"
     gene            complement(9418..10431)
                     /locus_tag="VGR29_04325"
     CDS             complement(9418..10431)
                     /locus_tag="VGR29_04325"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEV2072850.1"
                     /translation="MPNAAITGWGVAVPDRVLTNQDLERMVETSDEWIVSRTGIRERR
                     IVGANDSTTSLATDAARKAMERAGCTADDVDLIVVATATPDDFLVSQSCLVQAEIGGS
                     AAAFDVGAACAGFVTALAVATQFVNGGVYNRALVIGVDTLTRYIDYTDRGTCVLFGDG
                     AGAVVIESSSEPRGLLSTELGADGAGHKHLYVPGWAQMVPESADIFPDSRPHLQMNGR
                     EVFRFAVQVMGDAALKSIEKAGLTLDQVDMLIPHQANARIIWAAQRQLQLPQEKVWIN
                     VDRFGNTSAASVPIALAEAADAGALKEGDNVVLVAFGAGLAWAAGLVRWGTDGTGPVT
                     PAS"
     gene            complement(10976..11542)
                     /locus_tag="VGR29_04330"
     CDS             complement(10976..11542)
                     /locus_tag="VGR29_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV2072851.1"
                     /translation="MATDRTDAIKLINDTRLNVASLLMEDVGSIRELELTLTSFPLDE
                     ELVASSVLGQFRLTRLRSGVLVTGTISGDVELECSRCLEMYDQPFTATLTEQFRQTVD
                     VRSGSGITPARKPSDDQDEDDDELGFEIDDAHEMDLTEMLRQNILLALPMRPDCGERC
                     PGPPEIPNEPEAQSDSRFAALEQLLDDE"
     gene            12091..12960
                     /locus_tag="VGR29_04335"
     CDS             12091..12960
                     /locus_tag="VGR29_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEV2072852.1"
                     /translation="MTGSHAHARGDVLRAYLVLPHAVPIMAVLVATMAFALVAAGGWP
                     GAVNMVCLLGAMLGGQLAVGAVNELVDVELDRLSKPHKPIPAGLVSHRGARAIVLVGL
                     LLMVAFSVRFSIEAFVLCALGNGTGIAYSIWFKRTVWSWIPYIVAVPLIPIWVWTALD
                     DVPIRMLAIYPLAVPAVIALQLAQSMPDIGLDREAGVRTLAVELGSQWAPRVAWTCVL
                     LPLAIAASAAAWVAERPEWIWVSCLISGALLASNVVIWRRNPHQGVMSAFPLIAAAVV
                     VLGVGWSVGIVSL"
     gene            complement(12962..13792)
                     /locus_tag="VGR29_04340"
     CDS             complement(12962..13792)
                     /locus_tag="VGR29_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEV2072853.1"
                     /translation="MDAVIQITGERYVAFTESDSAANGGITEKEADTQLRSIQSELRE
                     LHDLMMAAETHALLIILQGMDAAGKDVTIENVLAEMNPQAFRVKGFKSPAGEEAKHHF
                     LWRANAALPMFGEAVVFDRSYYEQAIRPRVEGELPESDSDKRHEHIKAFERILDESGT
                     IIVKIFLNIGKDVQEQRLEERQNDIRSAWKISGSDWEDRAQWDEFMQAYETTLNACAS
                     PDLPWYLVPADHQWFHNVAVGQILLDRLGPFRQEWEAARRESGEQNQREAEAAHREAE
                     "
     gene            complement(13969..14487)
                     /locus_tag="VGR29_04345"
     CDS             complement(13969..14487)
                     /locus_tag="VGR29_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072854.1"
                     /translation="MNDRGGTLHPSDSAADILYLVDQLEELVSLGKRVPMSRRVMVEE
                     EDFLALVDQLRVALPNEIKQAQRVIKERERVIGEAQDEASRIVQGARERAEKMVSQNV
                     IIAEARQRGEEILRQAEEEQQRTRGELDVFVLRQLQLVEDAVRRGMAVMEDAVEQTLE
                     TVEDAKDAVGQE"
     gene            complement(14609..15142)
                     /gene="coaD"
                     /locus_tag="VGR29_04350"
     CDS             complement(14609..15142)
                     /gene="coaD"
                     /locus_tag="VGR29_04350"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV2072855.1"
                     /translation="MTIAIYPGSFDPLTHGHLDVALRAARLFETLVIAIYEGSNKPSA
                     MFPIPERITLAREAIADCEMPFANRIIVDSFSGLAVEYARTKGATTIVRGLRAVSDFE
                     YEFQYAHMNEHLAPEIEVVSLMTASRYSFISSSFIREVAMLGGSLDGLVPPNVERALR
                     GRTGQASTSPVLSASAT"
     gene            complement(15139..15732)
                     /gene="rsmD"
                     /locus_tag="VGR29_04355"
     CDS             complement(15139..15732)
                     /gene="rsmD"
                     /locus_tag="VGR29_04355"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV2072856.1"
                     /translation="MRIISGSARGRRLKGPPSHATRPMTDKIKGAVFNSLASLGVDPD
                     SVLDLFAGTGAIGIEALSRGASHADFVDMGREPCAVIRDNLRTSGFDRAGHVHQMSVT
                     TFIKRSNRAYDLIVMDPPYADPEIISTLEKLAESQLVQSGTIVVLGHSPRVTPPDQVG
                     SLEALRHRCHGDTCFFIYEAVDAIEGRPDSSSVEETR"
     gene            complement(15987..18497)
                     /gene="recG"
                     /locus_tag="VGR29_04360"
     CDS             complement(15987..18497)
                     /gene="recG"
                     /locus_tag="VGR29_04360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV2072857.1"
                     /translation="MPPSRAPRLPSRPSTTSRVQYLERAIDALRRVWVGGPDLGPDIA
                     IAVDALRHLTHQIASTHEIAATLESTIDSLMHLSREESARRQEVELLGKRLQAIKPHL
                     GQNESPRELGKLNSALGEKRVGHPIREPAPRRVATIGQQPPETLLSDLPGFGPKRIKP
                     YEDNLQLHTLEDVLRMVPRRHIDYSHTVRLDDPLGLRGEITVRGKLVEMREIRVGTPR
                     VQARLFDGTGSLRITWFSTYITKQLAEGDEIVVSGAVMPGYGGLQITNPEWERVGSEG
                     LSTGGLIPVYSLTKGVTQKSLRKLTRNVLDATKKQLVDWLADAREFFPENVWNALPPL
                     DQMYEHLHYPPMREDFERARRRLMFENLFLLQLGLVQQKKRRKQEKGRALSVEKSTLD
                     GFYSTLPFHLTGAQTRAVGEILTDIRKDQPMTRLLQGDVGSGKTVVAASVALVACGNQ
                     MQCAIMAPTELLAEQHAASLESLFAPLPEELRPRVALLTGSTRTAQRREILAALVDGQ
                     IDLLIGTHALIQDDVTFQSLGLVVIDEQHRFGVRQRGLLTTKAPGFQPHVLSMTATPI
                     PRSLNLVLHGDLDVSIIDERPPGRIPIETIRYTAQDRAQAYQIVREEVAGGHQVFVIC
                     PLVEESDMIEAKAAVDEAQRLQEEVFPDLRVDVLHGKMSGRIKDEVMSRFRDREFDIL
                     VSTSVIEVGIDIPNATVMMIEGADRFGLAQLHQFRGRVGRGGNKSYCLLLADEVSMDG
                     GARLHTMVASDDGFVLAERDLELRGPGDFLGTRQSGLPELGWLDQGFDTRILDLAREC
                     AERLIEASPNIDVARFQRLKPRLQRFWAHRQEPDSRLS"
     gene            complement(18599..19450)
                     /locus_tag="VGR29_04365"
     CDS             complement(18599..19450)
                     /locus_tag="VGR29_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV2072858.1"
                     /translation="MTETREVLIVTDSTSDIPGSLRDQYDITVVPLNVTFGSDTFQDG
                     VDITPPQYLQRLTQEKDLPKTSQPPSTRFASVFNEAIDRGMDVLCITLSSQLSGTYNS
                     ARLAAEEFDSERIRVVDSRSTTMQLGWIVIEAARAVQSGAGLDEAALRATEAIGRANC
                     FAVLQTLDYVYKGGRIGRASHIVGSALGIKPVLNFVDGILTPIERVRTWKKALARAVE
                     LAATTGDPQDIAVLHSDNLKDAETTADALRQRLPDASIIIDWTGSTILTYAGPGAIGI
                     LTLGKRP"
     gene            complement(19443..20300)
                     /locus_tag="VGR29_04370"
     CDS             complement(19443..20300)
                     /locus_tag="VGR29_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV2072859.1"
                     /translation="MSQRSSVAVVTDSTSDLSFEVAAAHDITIVPLNVHFGDEVFRDQ
                     IDITTDEFMARMAAANTLPTTSQPSIGAFETAFREVAETHSEIVCVVLSSKLSGTYQS
                     AQIAAMNVAETIKVELVDTLNVTYGLGFQVLRAAELASNGQDATTIASTLRSELGHYH
                     VVFFVETLEHLRRGGRIGKAAQLVGSLLQLRPLLRIDEGQVVPFERTRTRGKAMDALA
                     AFAGSVGVPEEIAVIYNTTPGDARKLADKVATLTPEHDVIIVQLGPVIATHIGPDVLG
                     IIVKERLSD"
     gene            complement(20467..22164)
                     /locus_tag="VGR29_04375"
     CDS             complement(20467..22164)
                     /locus_tag="VGR29_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV2072860.1"
                     /translation="MQQPIRGTSLSDMWNGDELLDALDFATKWLDHNRDRVNALNVFP
                     IPDGDTGTNMALTMHASLDAARNADNPSSASSVASKFAFGSLMGARGNSGVILSQIFR
                     GFGAAITDRDTIDGHDLAKALEGAREMAYKAVMRPVEGTMLTIIRVAAERAGTTAHRT
                     PSLGAVLKDSLDAANEALAQTPDMLEILRRAGVVDAGGQGVVHILEGLYRYTQGDTAL
                     VEDLSAAGIGADMRFLDDVTETHGEDPFGYCTNFMVFGEGIDFGTVRSEIAAMGQSVV
                     VVGDDTVVKVHIHTENPGSVLEYALTLGYLDQIKIDNMTLQTSALTDQRQQARDKAVP
                     AHASNPDVAQSIDGDIGVIAVAAGDGLAAALRSMGADCIVRGGQTMNPSTEDLLQAVE
                     ALPVDQIIILPNNKNIVMAANQVAGLTEKTVRVVPSRSLPQGLSALAAFNRDASLNVN
                     EQVMSSALEDVHSIEITQAVRDVELNGVTVVKGHLIGLVDGDLVASGPDLQQILESVL
                     TSLQDSEPELLTVFVGEDATEPGTEALRATAEEVFPEAEIEIVEGNQPHYRYLIAVE"
     gene            complement(22167..22553)
                     /locus_tag="VGR29_04380"
     CDS             complement(22167..22553)
                     /locus_tag="VGR29_04380"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV2072861.1"
                     /translation="MEHHEPLQDSAPAGRVEIAESAIIGIIHETVLSCYGVVDMGPRS
                     LGSAIVKRLGIGNGPRGIGIQNEDGHLTIELSVVVEYGTPIFTVAQNVMQTVKFQVER
                     MLGMDVDRVNVNVDGLRVSSPSGSAV"
     gene            22846..23049
                     /gene="rpmB"
                     /locus_tag="VGR29_04385"
     CDS             22846..23049
                     /gene="rpmB"
                     /locus_tag="VGR29_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV2072862.1"
                     /translation="MAGKCDVCGKAKTFGNNVSFSKRRTNRDFRPNVHKKRIIVNGVG
                     IRLNICTRCQRTLGKAGKTKVAS"
     gene            complement(23110..24297)
                     /gene="queG"
                     /locus_tag="VGR29_04390"
     CDS             complement(23110..24297)
                     /gene="queG"
                     /locus_tag="VGR29_04390"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEV2072863.1"
                     /translation="MDVAASPLTLHTAPTTDRQRLTAQVKYLAARHGLAVSAVTTADP
                     FPAVASQLEAHIEAGHVEGMDWFTQERARVSSDIRNLHPTAQSIISFGLAYWSGPAEK
                     PVDGVPRGKISRYAWGKDYHRVLKRRMKALLSDLNELAGRELDARILVDTARVVERAV
                     AARAGLGWYGKNANLIVPGRGSWVLLGEMVTDLDLEPDQPLDRNCGRCTICIDNCPTG
                     AIVEPYTIHAPSCISYLTIELRDAIPYQLRPQMGDWVYGCDVCQEVCPYTGAAEIVND
                     PDMQPRSLNNAFPSLHWLLTMTQDEFGETYFGTPVPRTKRRGLARNAAVALGNIGTRD
                     DVPTLAGAAISHDEPIVRGHAAWALGRIGGPVARTTLDRARTLEPDTSVQDEIALALE
                     QVT"
     gene            complement(24319..25107)
                     /locus_tag="VGR29_04395"
     CDS             complement(24319..25107)
                     /locus_tag="VGR29_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072864.1"
                     /translation="MTKRYAVIGDIIAEAHNTTLRPGGSAAIALAIAMLGGSIKLRSV
                     LATDDPGNEVLSILKQARIHPGLLDKTDGATTAIVRRNDDGEVIERRDGIGIEKGAIM
                     DIYDLFGHDALVLDARDQRLRRFLSDLPAHTDGNVRMIGTLGHLDWNEPSIDEMEIAL
                     RFDTIVGTANQYAKLTGRPSPTDALGDIFDRMPGTHLRAAAAVTPDGLELIAREDRVL
                     RPIQHVVPDLLLPQAVAGIAWGLAHRADWDIAATAAADPSQITK"
     gene            25254..25796
                     /locus_tag="VGR29_04400"
     CDS             25254..25796
                     /locus_tag="VGR29_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072865.1"
                     /translation="MGSVVRFIEGGDAEIVDANKLNTPVDDPPSGRGVDTNERGIERP
                     RARTVPGAGVSRFQQWVSDAPGNVGLLQIGSCNDIDSFGDASDVAPARPACDRNLLET
                     LPVLQIVPVRINMGARMGTKRNDARVEFIVGDRRVRFESNIGISRLCEGAGLDGQCNV
                     VDAWCAHSLYRFPPILPTIL"
     gene            25839..26615
                     /locus_tag="VGR29_04405"
     CDS             25839..26615
                     /locus_tag="VGR29_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2072866.1"
                     /translation="MAYSWGVESKWKVPFPAIDREDGWLIRYGDDRRKVPVSLPLARR
                     PLSAARRRQRNERCAAILEAAGRLIARDGLSGLSMRLLAKELGVPAPTLYGYFDSKEA
                     VIDALAQEKIALLREAILSEAKDASPGVPRLLAFARGYRTFALSGRDYYDLFILKATP
                     DGQDARTVDNTAGMELIQTLAEDVQTAIDLGQLQPIDPEHTILALWAVAHGYVSLEVS
                     GALDDLDSSPVSRERTYLLYFESMLRGLEVCAPPQPTSSE"
     gene            26653..27144
                     /locus_tag="VGR29_04410"
     CDS             26653..27144
                     /locus_tag="VGR29_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2072867.1"
                     /translation="MARPRTTKRTTPTAGSLLGEWVRRARQEQGLSQRALADQSGLSR
                     SYVCDIERGRGAEPSLTTLDKLAAALGASRADLMRAAGLIDRALVPRESEEERRLLSV
                     YRDLSDEGQLNILRFARFLHQEEHAWTQISLVGDNGAEESAPPPTPDARRPALFSLDD
                     GNG"
     gene            complement(27334..27831)
                     /gene="rpmE"
                     /locus_tag="VGR29_04415"
     CDS             complement(27334..27831)
                     /gene="rpmE"
                     /locus_tag="VGR29_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEV2072868.1"
                     /translation="MKPGIHPNYQEATVVCSCGNTFTTRSTKSNLRTDLCNVCHPFYT
                     GEQRIVDTAGQVERFTKRMEAAQSTSGVKSKRQRRAEELARTRAEAEAEAAAQREAAA
                     KEERAKRRAEREAAETARAEREAAEAAKAEREARQVQAASAGQTSTDASPENVLAEEA
                     ADSEK"
     gene            complement(27870..28196)
                     /gene="rpmA"
                     /locus_tag="VGR29_04420"
     CDS             complement(27870..28196)
                     /gene="rpmA"
                     /locus_tag="VGR29_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV2072869.1"
                     /translation="MAHKKGMGSTRNGRDSESKRLGVKRGDGQYVRTGTIVVRQRGTQ
                     YWVGNNVGIGKDHTIYSLIDGVVRFETISRQKRRISVYPPEIYPVGGATELTSAPAAA
                     EVTAAD"
     gene            complement(28255..>28642)
                     /gene="rplU"
                     /locus_tag="VGR29_04425"
     CDS             complement(28255..>28642)
                     /gene="rplU"
                     /locus_tag="VGR29_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV2072870.1"
                     /translation="AESTEDSAPATAPSKKKSKTAPELSPYAVVETGGKQYRVSVGDH
                     ITIERLADDAGSEIILDRVLMLGGDGSTRIGTPVVEGATVSATIDEHKRGEKIVVFKY
                     KAKKGYRRKMGHRQELTRVTITGITG"
BASE COUNT         5550 a         8303 c         8845 g         5944 t
ORIGIN      
        1 gcagtacggt ggttttccag acctacacgc ccgagcacta tgcaatccgg gcggcctcca
       61 ggcatgacta tcaagccttc tacgcagatg agatcacctt ccggcaggac ttcaggttcc
      121 cgccattcct gcgtctggcg cgattcatgt tccgtcacga caaggaggag ggtgcagccc
      181 tggaagctgg gctgctggca cgggagctgg cacggcatgc ccgggtcaac gacatcgaga
      241 tggagcttct gggaccaaca ccagcgttcg ttgccaagat ccgcaaccag taccagtggc
      301 agattgtctt gcgatcccgt cagatggacc ggatgctgga aggaatgccg atgcgtccag
      361 gttgggtcgt cgatattgat cccgagagta tgctttaagc agggtttgtg gcgcgcggaa
      421 cgatggaaat tcgtcgctat ggggtagatg gaatggcgat tcttgatatt gtgctcgaag
      481 gcgacccggt acttcgcaag aaggccgtga aggtgccccg cgcggatgat ggcatccgca
      541 agcttgccgc caatatgtat gaaacgatgc tcgaggcacc aggtgttggc cttgccgctc
      601 cgcagatcgg caagagcatt cggctgatcg tggtacatgt gccagaggat gaagacgatg
      661 aaactccaga agtttccctc aaactgctca acccggaaat catcaaggcg tttggcacgc
      721 aggtcggggc cgaggggtgt ctgagcatcc ccggttgggc tggcaatgtc gagcgagcaa
      781 tgaatatcac cgtgaaggcc acggacatga acgacaagcc ggtccggatc agggcacagg
      841 ggtatctcgc ggtcgtgctc cagcacgaga tcgaccatct cgatggcatc ctcttccctg
      901 accggatcga gaacacgaag gaagatctgt ggccggtgcg ggagagcgag gaagaactcg
      961 tcgaagacga agggatcccg gtcgcctagt gaccgatcag taacactcca ggttcgcctc
     1021 tgtggaacgg tacaatcccg gcacatacag ctgtttgcag agaggtcgcc tgtctgtagc
     1081 ctgtagcgaa gggaaccgct gccgctattc gcccgagggc tcacccagga attgcggcgc
     1141 atgctccctg ttcgcgcccg cgaatgcagc aatgtcctcc tggcggagtg cgagggacga
     1201 catcgctacg gtacgtcgtc cgctacacta gactctctgg atcccggacc ttgaatatca
     1261 gtctcattac aaatcgctgt agggtagtcg tgtcgacccc atgtcgatcc gaccgacagc
     1321 atgggagtac attggggtta cttgtacggt gacgtcattg aagagtggga gcgatccgcc
     1381 gtcactcaga gagactcgca gtcgagctac gggatttcga tttcgggctg gtggaccttg
     1441 cggtgctcaa cggaaacgat gctgcagatc tgttccacgg cgccgtcgag cttgccatcc
     1501 tcgttgaaaa ccacgtagtc gaagttctgt gcttcctcaa gttccgcggt cgcggtggtg
     1561 aaccgcttca tcagcacctc gggatcgtcg gtcttgcggt cacgaaggcg gttaagcagg
     1621 gccggcatcg attccgggca gaggaagagc gagatggtgt tcgggataag gcggcggagc
     1681 gtcgcggctc ccttgacgtc gaccttgatg atgacatcat ggccctgctc aagtccccgg
     1741 acaatcgggc tgcgtggaac gccatagtgg ttgtcgtaga ccagagcgtt ctcgatgaaa
     1801 tcattcttct cgatacgatc gaggaactct tctttcgaaa gaaagatgta gtggacgccg
     1861 tcaatctcgc cgggccgcat ggggcgagtc gtggccgtga cgacatattg cgcatcagtg
     1921 aagacgtttc gcagcgattc gatgacggaa tccttgccga cgcctgaagg gcccgagatg
     1981 ataaagacac ggggcttgtt tccctttcgg atgcccgtga tcagggcatt cccctccgcg
     2041 tagagctgcc caagtctggc atcatggtct gaaggaaatg tttcagacat tgctgatgtc
     2101 gccggcgtgt tctgtgccaa gaggagtttc tccctgttgc gagcgtccgg ccgggtcccg
     2161 tgatgccgga gaagatgcat ttatcaagag ttagggtaca cgagaatgtt tgatgtgttg
     2221 accgcaggtg cagtggtaga cgagttgcgc cgctcagtcc ttgatggacg gatccagcgg
     2281 ctggggcttg tcgatgcctt caccattggt gccgagatct atgctcaggg acgccgccgg
     2341 catctgatcg cgagtgcttc cccgcagaat ccgaggattc atctcactcg atcgatgccc
     2401 tctaccgatc ccaacctgat cacgccgttc gggctgcagc tacggaagta cgtccgggga
     2461 gggttcctga ttggtattga gcagccaccc cttgaacggg tgattcggct cagtatagcc
     2521 aagcgcatgg catcactcaa ttcatcggga gcagaggagg gccctgacga cgaggaggag
     2581 ggaggggagg acgatatctg gtcgggccag gatgtctcac ggttggagct ggtgatcgag
     2641 atcatgggcc ggcacagcaa ccttatcctc gttgacgacc agggcctggt gcgcgagagc
     2701 gccaagcgcg tcacctcatc catgagccgt gtgcgaccaa ttcttccaaa ggtgccctat
     2761 gatctgccac cggcacccga caagccagac ccgcgacgcg taacctcacc gtccatggag
     2821 caatttctgg tcaatgcgaa gccggacgcg aagcttgctg atctcctggt tcgcgggtat
     2881 cgcgccatca gcccgcagat agcccgtgaa gctgcctttc gcgctgctgg agaccctagg
     2941 gtgaaggtcg cggcactggg ccaggaggac gcgcgggaga tcgcccaggt cgtgcgcggg
     3001 atcttccagc ccttactcac cggcgaatgg gagccacatc tgtatgagag ggatcatctt
     3061 cccatcggct atggcgctgt ccccataagg tatcttgcgg gcgaggccga ggacgtgcca
     3121 ctcgaatcga tctccgaggc gattgagcgg gcccaggatg cgacggcaga ggttacgccg
     3181 aaggatcacg cgcaacggcg cgctcgactg ctccaggcaa ttgacgcggc catcaagtcg
     3241 gtggatacac gtatccggtc attgcaacag cagcatgagc gatcacgcga taccgagcga
     3301 ctgcgtcgct ggggtgagac gatctacgcc tatctctggc aaatacaact aggagatgcg
     3361 gagcttatcg tggagacgga agcaggcgag gagaagattc cgcttgatcc ggcgaaagat
     3421 cccaaagagg ttgcccagga gtatttcgag gagtaccgga aggcacagaa ggctggcaac
     3481 acgctcccgg agcggatcga agcggccgag aacgagcggc ggtatctcca gcaactgcga
     3541 acgcagtctc agcaggccga aggatttgcc gcaattgagg cgcttcgaca ggagtacggg
     3601 gagcatagtg gtggccggca tgctgtcgag gagaaaccag ggcacaaggc atcccgaaag
     3661 cagtctgcgc ggaaggtcgc cccgctcacc gatgacgacg gtaacctgat ctatatcggc
     3721 cggtcggggc gtgagaatga gcaggtcacc ttcgagatcg ggggatcgga tgacctctgg
     3781 cttcatgcgc gtggggtacc cggttctcat gtgatcctcc ggatgcgagc gccatcccag
     3841 gagccggaca acagtgccct cgagaccgcg gcgtcgctag cggcgtacta ctccggctcg
     3901 cgggagagtg gcacggtgga ggtcgatgtc accgcgcgcc ggcacgtacg gaagatcaag
     3961 ggaggtggtc ccggcatggt gacgtaccga aacgagcgga caattgcggt aaggccgtcg
     4021 gacgaatcgc agcttcgcaa ggccgggcag atagagtagg gatcagcccc cggcctgcgg
     4081 atagtgtcac acagcttttg gcaggtgcag caacacgctc tggaggcttg tggtccttcc
     4141 ttccatagga tcggggaggg tccgttcaca ggggtaggcc gacaagaacg acccgggctt
     4201 ttgcccgtgg tcgttctcct tttcgatctt gtagcgatta ctagatgtcg tcggcctttt
     4261 cgatccggct ggggacgagc tcgacttcct gggcatcgtc gctggagaaa ttctcaccgc
     4321 gcagacctgt cttggcgaag atcgatgaga tgatgatccc gaattcatag aggatgatga
     4381 gcgggattgc cacaatggcc atgttatagg gatccgtgct tggcgtgatg atcgccgcag
     4441 cgaccagcag gagcatgaat gcgtacttgc gccacttgcg catctgccga ggcgtgatca
     4501 caccgatctt ggcgacgatg aacatgatca ctggtaattg gaacgcgagc cccagtccga
     4561 tcatcaaggt catgaagaag gaaacagtct cgtttccgtc cgggttccag tcgaacgcgt
     4621 tcgtgttcca gttcgacagg aagtacaggg cacgaggcgc ggcgatgaaa tatccgtaat
     4681 acacgcctat ggcaaagagg atggtcgtga agggtaaggc gctgaagagg acgcgctttt
     4741 ccttgcgcgt catgcccggt gcaaggaatg ctacgaactg atatacgatg accggcatac
     4801 agatcgcgat ggcgacgtag agggcaatct tgaacgtcag cgtcagcgtt tcagtaggag
     4861 acttgacgtc gagtccatca acagcattgg ccttgttgct gatgtccgag agaatgcgac
     4921 cggcgataaa gaagccgaag aggaacgtcg ggacgatcgc gatcaccatc ttcatgatgc
     4981 gatcgcggag ctcctcgagg tgctcctgca gcgtcatctc gtcaaagacg tccggctcgt
     5041 ccgtgatttc agggagacgg ggaactctta tttttccgaa aggaagactc ttgagggtgc
     5101 gcgccatgac ggatctttct ctcctggatg atcctgccca aagtccgggc agatgatcgc
     5161 tacctgtgga gtgtctcagc gcgaggttgg accatgtatc tcagcagcgt tatggacgac
     5221 caggatggat acacggttcg tcggcacccg ggcgatccgg ggtatccggg aggagaaccc
     5281 cccgcattgt tgccattggt cgttcgctcg caataggcag gtgaatgcac gtgaagatgt
     5341 tattccgcac gcgcattcgt ccgcggccag aaccagaatc aggattctag gatgcggaac
     5401 tctgctcgat gtagtttacc gcatcaccga cagtacggat cttctcggcg tcctcgtcct
     5461 tgatctcaag gtcgaactcc tgctccatcg ccataatgac ctcaacgagg tccagcgaat
     5521 ccgcattcag gtcgccaatg aactcggctt ccgggacaac ctgttcggcg tcaacgccaa
     5581 ggcgatcggt gacgatattc tgaacacgct caagagtggt agccaagact gtccctcctc
     5641 attcgagaac tgtttgccat gggttcctca agatgaggag aagcccgctt tcacaatagt
     5701 gcagcttttc cagcgtcaca cctgtgaatt gccgcccatt cgggcagttc aggtgctgca
     5761 caacacaaga agcatacccg ggtgattcta catgaaccac acggcaaggg gtaggcaaac
     5821 ggaggtttga ccttgcggtg gacatgctgc cagggcctct cggcttgacc cgtgggcggg
     5881 tccatggtta ccaggcatct ggcctagcgg ctgctgtgct ttcgtttggc aggaagatcc
     5941 ttgctgatgg tgccaaggac gccattgaca aagcgcccgg agttggggcc gccgaagcgt
     6001 ttggcgatct ccactgcctc attgatgacc accttgtaag ggacatccgg cgcgtagagc
     6061 agttcgtaga tggcaagccg aaggacatta cggtcgatcg cggcgagctg cggaatggga
     6121 aattgggggg ccgccttttc gatcaacgga tcaatcccgt cctgatgctc gaggatgccg
     6181 tgaacgatgg ctgtagcgcg gtctgccact gactgtggga tgtcctccag ttgggtttca
     6241 tcctcgtcgg tggtttccgt cgcggatgct ggcagatcga catcgtcgcc aattcgcatc
     6301 cgttcgagga tctcgtgcag atcatgttct gtcaggtcgc gctcgtagag agcctgcatc
     6361 gccaggacgc gtgcctggtg gcgcaccccc gacatgtcga catacgcacc cttcccgaaa
     6421 ccagccggga cggaggcttt ccccttccgg gggcctttcg atcccgacct gttggatgat
     6481 tgcttcttcg gttcgggatt caaaggttct ggacgctcgg atgggtccgc gctcatacgt
     6541 gagactcgat tccttgatta tcatgattgc gttgcgggcg atcaggcagc cggatcgaca
     6601 atgtcgtcga tgaagatgtt gacgcttcta acggtcatgc cgagcatacg gcccgctgcg
     6661 acgccaacct tgcgtcgtat ctcgtccgca agggtagaaa tattcgtgcc acggcgaacc
     6721 gcaatagaga tgtccgcgtc gatctgatca ccatctacgc agacgcgcac cttgccgtcg
     6781 tcaaaggact tgccgcccaa tggcggcgtc tcgtcgttct tgctgcgttt gcccggttta
     6841 agctcaagca cacccgagat atccgagacg gtgacctcaa tgagctgaat caggaccgca
     6901 ggcgcaatgc ggacagtgcc gcggacttcc gttgcatcgt ctgcctggaa tgtcgcgttc
     6961 acctccgcct tgtcgcgaga gggtgcgatc aacttgtccg gcgcctgatg gtctgtcatc
     7021 cgcgttatct cccgatcggc aatcgctgcg gcatggcgtc ttcttcagcc gcatccttga
     7081 gacgggtacc caggcggctc atcaactccg gaatgaaccc ggtatggacc tctcctcgct
     7141 ggaaggctgc gtcggcgaga atctcccggt ggaacgggat ggtgtgggtc acgccggtca
     7201 acaccgtctc acgcaaggca cgctccatcc gcgcgacggc ctgctcccgc gtggtgcccc
     7261 acgtgattac cttgccaagc aacgagtcgt aattcggggg aatggagtag ccactaaaca
     7321 agtgtgaatc catccgaacc cctggcccgc cgggagcaac ataggtttcc acggtgccca
     7381 cagatggacg gaactcgttc gcggcatcct cggcggtgat gcggcactcg atggagtggc
     7441 cacgtggctt gagctgctcg ggatgaatgg tgagcctctc ccccgcggca acccggatct
     7501 gccaggccac cagatcgagg ctggtgacct cctcggtgat cggatgctcg acctggatgc
     7561 gggtgttcat ctccatgaag tagaagtgac cgtccgtatc tagcaagaac tcaagtgtcc
     7621 cggcgctcca gtagttggcc gccctggctc ccttctcggc agtctttaac aggttttggc
     7681 gcaccttgtc cggtagatga ggtgccgggg cttcctcgac aaccttctgg tggcggcgct
     7741 gcagcgagca atcgcgttcg ccgatcgcta cgacgttccc gtgctggtca ccgatcacct
     7801 gtacctcaac gtgacgcggt cgctgaaggt agcgctccat atacacatcg ccattgccga
     7861 aggcgctctc tgcctccgac tgggcaactg gatacccacg ggtcaattcg gcatcgttgg
     7921 tggcaacgcg catgccgcgt ccgccgccgc cagccacggc cttgatgacg actggatacc
     7981 cgatctcccg ggcggcctgc cgggcatgtg tcagagtggg cacgatgcct tgcgtgccgg
     8041 gaagcagggg aaccccggcg gctttcatga ccttccgggc ctcagcctta ttgcccatct
     8101 tctcgatcac gtccggcggt ggcccaatga aagttaggcc gacctgctgg cagatctcgg
     8161 caaggtaggc gttttccgcg agaaagccgt acccgggatg aatggcgtcg caccccgtaa
     8221 caagcgccgc gcttatgact gctgggatgt tgttgtagga gcgtgccgcc ggcgcggggc
     8281 cgatacagac ggcttcatcg gccagacgca ccggcaatga atcgtggtcg acatcgctgt
     8341 acgcaacaac cgcagggatg cggagatcgc ggcaggcccg tagaatccgc agcgcgatct
     8401 caccacgatt tgcaatgagg atcttcttga acattcagtg gcctccttcc cgaaaatagc
     8461 gtccggggcg ttcagtggtg cagtaggggc cccggcagtt tgtcagggcc gtgaggcgag
     8521 cttcgccagt gatgatgggc agcatggaac atgccctgca actatcacgt gccctaggat
     8581 gccatgaaga gtccaccgtc gacggagagg gtgttgccgg tgatgaaccc cgcgtccgga
     8641 gaagcgagaa aggcgactgc gtttgcaaca tcctcagggc tgccgaagcg accgagtggc
     8701 gtattcgcca ggaggttctg cttcaggtcc tcggggagca cgtccgtgag acgggtgtcg
     8761 atgaacccag gggccacggc attgacggtg atgccgcggg agccgacttc ctttgccagg
     8821 gatttggtga gacccaccag accagctttc gcggccgcgt agtttgcctg gccggcattg
     8881 ccgacaagac caacgacgga ggtcaggttg atgatgcgac cggagcgttg ccgaagcatg
     8941 ggacggactg cggccttgct ggtcaggaag gcgcccttga gatttgttgt cagtactgca
     9001 tcccaatcct cctccgtcat acgcatgatg aggttgtcgc gcgtgatgcc cgcattgtta
     9061 accagcacat cgatggagcc gagttccttc accgcggtat ccacggtcag ttgcgcacct
     9121 tcctgggtgg agacgtcagc cgtgatcacc ctgacactcc ggccagatcc tgccagttcc
     9181 ttttctgtcg ctgccgcagc ttcagtatcc ccgcggtagg tgagaaccag gtcaaatcca
     9241 tcctttgcga ggcgccgggc aattgcgagc ccaatgccac gggtgccgcc ggttaccaca
     9301 gccacgggtg agcggagtgc ttcagtcatt gggggaactc ctcttgtgtt cgcaaggtgt
     9361 gagacctgcg ttgcgcgggc cgtgtcaggc gcaggtattt attgccgcga gctggctcta
     9421 ggatgccgga gttaccgggc ctgtgccatc ggttccccag cgaaccaggc cggcagccca
     9481 cgcgagaccc gcgccgaatg cgaccaggac gacattatcg ccctccttga gcgcacccgc
     9541 atcagcggct tccgccaggg caatcggcac agacgccgcc gatgtgttgc caaagcggtc
     9601 gacattgatc cagaccttct cctgtggcag ttgaagttgg cgctgtgcgg cccagatgat
     9661 ccgggcgttg gcctggtgtg ggatcagcat gtcgacctga tccaacgtaa gaccagcctt
     9721 ctcaatagat ttgagcgccg cgtcacccat gacctggacg gcgaagcgaa agacctctcg
     9781 cccgttcatc tggaggtgtg gccgggagtc ggggaaaatg tccgcggatt caggtaccat
     9841 ctgcgcccag ccaggaacgt agagatgctt gtggcctgca ccatccgcac ccagctccgt
     9901 cgaaagcagc ccacgtggct cggatgagct ttcaatgacc accgctccag cgccgtcgcc
     9961 gaagaggaca caggtgccgc ggtccgtgta gtcaatgtaa cgggtcagcg tgtcgacgcc
    10021 aatcaccagc gcacgattgt aaacgcctcc gttgacaaac tgggttgcga ccgcaagcgc
    10081 cgtgacgaac ccggcacagg cggcaccgac atcgaatgcg gcagcgctcc cgccgatctc
    10141 ggcctggacc aggcaggact gcgaaaccag gaaatcgtca ggtgtggcag ttgcgacgac
    10201 aatcaggtcc acatcgtcag cagtacaccc ggccctctcc atggctttgc gggccgcgtc
    10261 cgtggcaagc gaagtggtgc tatcgttggc gcctacaatg cggcgttcac ggatgccggt
    10321 gcgagagacg atccactcat cgctcgtctc gaccatccgt tcgagatcct gattggtcag
    10381 cacccggtcg ggaacggcaa ctccccaacc ggtgattgcg gcattaggca tcgacagtcc
    10441 ttgctcgtgc caagtgtcgg gtccgtcctg tacggttgca tcgcattgcc gggcagggac
    10501 ggaggggccg gggacaacgc cgggatgaac gaagttcaat cacatcatac cgggttcatg
    10561 gctggaacgc gcaaagagaa aggcgggaga gctcgcactc accccgcctc tgcttgcttg
    10621 cgccgcgccc agtgtcatga acctctcggc tcgggactac cgggagctgc tgggacgctg
    10681 gcgctcctca atgtcgatca cctggcgtcc ccgatactga ccgcatgtcg ggcagacgtg
    10741 gtgcggctgc ttcatggagc ggcagttgct gcatggcaca aggttgggag ccgcaatgaa
    10801 atggtggcgg cgtcggttgc cctggtgggc tcggctaatt cgttgcttgg gaagcgcacc
    10861 catcgttgat tctcctctaa agttgcacct gcggcaggct ggctgctacg agatgactga
    10921 tttcaactgg tatattgcca cgtcggcgat atcgtcctgc gtgtgaacaa cgcgttcact
    10981 catcatccaa gagttgttcc agcgcagcaa accgggaatc cgattgagcc tctggctcat
    11041 ttggaatctc cggcggccct gggcaacgct ctccacagtc cggacgcatc ggcaatgcca
    11101 gcaggatgtt ctgtcgcagc atctccgtca ggtccatctc gtgggcgtcg tcgatctcga
    11161 atccgagctc gtcgtcatcc tcgtcctggt cgtcggaggg cttacgggct ggcgtgatgc
    11221 cggagccgct tcgcacgtcc accgtttgcc gaaactgttc ggtcaaggtt gcagtaaaag
    11281 gctgatcgta catttcaaga catcgactgc attcgagctc gacatcaccg ctgatcgtgc
    11341 ccgtgaccaa gacgccggac cgaagccggg tcaagcgaaa ctgcccaaga acgctgctcg
    11401 cgacgagttc ctcatcgagc gggaaggagg tcagcgtcag ctccagttca cggatgctgc
    11461 cgacgtcctc catcaggagc gatgcgacgt tcagtcgcgt gtcgttgatg agcttgatgg
    11521 cgtctgttct gtctgttgcc atgaaatacc acccttcgta cgagggcccg gatgcacctg
    11581 tctccggcga tacgcacgaa gacgccgggg attcctcccg gcgtaaccgt gacagtatac
    11641 gcggaggctg gcgggagggt caacatgccc tccgcgagac ggaagattat cgatgacggc
    11701 cccatagccc agtcaccaga gagcagcgat tctcccccag tcggagatca gggatcagga
    11761 ggcgacacag cactgatcct ccaggtccaa cactcggttt aaatgacacg aacgagggtg
    11821 tctgcgaggc catttttcaa gtgctggaac tggatcccag cgtcttacgg atatggggat
    11881 catgaaatgc atttcccttc gctgtggcgc aactccgtat ttgccacgat ttctggggtt
    11941 ttggttcgtc tgcagcggga gcgggtagtg aattgttcgg tgggggatca gaagccgcta
    12001 tgatgcttgt atgtacgacc ttccaatccc taatcctcgt tacgggggga cgactgtgat
    12061 ttcccaatca gacagcccag gtagggtgcg gtgaccggaa gccatgccca tgcacgcgga
    12121 gacgttctac gcgcctatct tgtactcccg cacgctgtcc cgatcatggc agtgttggtt
    12181 gccacgatgg cattcgccct tgttgcggcg ggtggatggc caggtgcggt gaatatggtc
    12241 tgccttctgg gagcgatgct cggaggtcag cttgcggtcg gtgcggtcaa tgagctcgtc
    12301 gacgtggaac tggatcggct ctcaaagccc cataaaccga tccccgccgg acttgtctcc
    12361 caccgtgggg cgagggcgat cgtgctggtg gggctgctgc tgatggtggc gttcagcgtc
    12421 cgattctcta tagaagcttt cgtgctctgt gcgctgggga atggtaccgg gatcgcgtat
    12481 agcatttggt tcaagcggac ggtctggtcc tggataccct atatcgtcgc tgttccgctc
    12541 atcccgatct gggtgtggac agcactggat gacgttccga ttcgcatgtt ggcaatctat
    12601 ccacttgctg tgccggcagt gattgcgctg cagttggccc aatccatgcc ggacatcggc
    12661 ttagatcggg aggcgggtgt gcgaacgctc gccgtggagt taggcagcca atgggctcct
    12721 cgtgtcgcct ggacttgcgt gttgcttccc ttggcgattg ctgcatccgc ggccgcgtgg
    12781 gtagccgaac gtccggagtg gatatgggtc tcgtgcctaa tctctggcgc cctattagca
    12841 agcaacgttg tcatctggcg ccgtaatccc catcagggcg tgatgagcgc attcccgttg
    12901 atcgcggcgg ccgtggtcgt tctcggtgtc ggctggtccg tggggattgt cagtctgtag
    12961 cttattccgc ttcccggtgt gctgcttccg cctcccgttg gttctgttct ccagattcgc
    13021 gacgggccgc ttcccactcc tgcctgaatg gtcccaggcg atccagaaga atttggccga
    13081 cggccacgtt gtgaaaccat tgatggtctg ccggtaccag ataccagggg agatccggtg
    13141 aggcgcaagc attgagtgtt gtctcgtacg cttgcatgaa ctcgtcccac tgcgctcggt
    13201 cctcccaatc gctgccggat attttccacg cactccggat atcgttctgc cgttcttcca
    13261 aacgctgctc ctgaacgtcc ttgccgatat tgaggaatat cttgacgata atcgtcccgg
    13321 attcatcgag gatccgctca aaggctttga tgtgctcgtg ccgtttgtcg ctgtccgatt
    13381 ccggaagctc cccctcgact cgtggccgaa tggcttgctc gtaataggag cggtcaaaga
    13441 cgactgcctc accaaacata ggtagcgcag catttgctcg ccacaggaaa tggtgcttcg
    13501 cctcctcgcc ggcaggcgac ttgaagccct tcacccgaaa tgcctgtggg ttcatctccg
    13561 caagaacatt ttcaatcgtg acgtcttttc cggcagcgtc catgccttgc aggatgatga
    13621 ggagagcgtg agtttccgca gccatcatga ggtcatgaag ctcgcgcagc tcagactgga
    13681 ttgacctcag ctgtgtgtcg gcctctttct cggtgatccc tccgttggcg gctgaatcgc
    13741 tttcggtaaa tgcgacatag cgttcgcccg tgatctggat gactgcgtcc aatgctgttc
    13801 tccgtgtacg agtgggcttc gacaaaaggc ttgggtggca cctacaccac gaataacccg
    13861 ggtgaaatag tcacagcggc gcagaacgca ccaagcgtgc ctgaagcgag aaaaacgggc
    13921 gggcgcctct tcgcacccgc ccgttcatcg actttctatg cgtaatgtct attcctgacc
    13981 gacagcgtcc ttcgcatctt caactgtttc cagggtctgc tcgacggcat cttccatcac
    14041 agccatgccc cggcggacgg catcctcgac gagttgaagc tgccggagca cgaagacgtc
    14101 aagctcgccg cgggttcgct gctgttcttc ctcggcctgg cgaagaatct cctcgccgcg
    14161 ctgccgtgct tccgcgatga taacgttctg ggagaccatc ttctcagcac gctcacgggc
    14221 gccctggaca attcggctgg cctcatcctg tgcttcccca atgactcgct cacgctcctt
    14281 gatcacccgc tgggcctgct tgatctcgtt gggtagtgcg acacggagct ggtcgacaag
    14341 tgcaaggaaa tcctcctcct cgaccatgac gcggcggctc atcggcacgc gtttgccgag
    14401 gctgactagt tcctcgagtt ggtcgaccag gtaaaggatg tcggccgcac tgtccgatgg
    14461 gtgtagtgtt ccgcctcggt cattcatcgc agggtccctc gtcgttcaga acacgtgccc
    14521 ttgccaacga cgtcgctcgt actcccagac gccggtgaga gcggtcgcag gcagcgtccg
    14581 ccgcgatcaa accgtatatg aaccctgttc aagtcgcact cgccgacaga acgggagatg
    14641 tgctggcttg cccggtacgc ccgcgcaagg ctcgctccac gttcggagga accaggccgt
    14701 cgagcgaacc gccaagcatt gccacctcac ggatgaaact ggagctaatg aacgagtagc
    14761 gggatgccgt catgagggag acgacctcga tttccggcgc aaggtgctcg ttcatgtgtg
    14821 cgtactggaa ctcgtactcg aagtcagaca ccgcccgaag gccgcggacg atcgtggtcg
    14881 ctcccttcgt gcgcgcgtac tctacggcga gtccggaaaa gctgtcaacg atgatccggt
    14941 tggcaaaggg catctcgcag tcggcgatgg cctcccgagc gagcgtgatc cgttccggaa
    15001 tcgggaacat cgccgacggt ttgttcgacc cctcgtagat ggcgatgacc agcgtctcga
    15061 ataaccgcgc agcacgaagt gccacgtcga gatgcccgtg tgtcagtgga tcaaagcttc
    15121 cgggatagat cgcaatcgtc atcgggtctc ctctacactg gaactatccg gtctcccttc
    15181 gatggcatcc actgcctcat agataaagaa acatgtatca ccatgacatc ggtgccggag
    15241 agcttcgagc gagccgacct gatccggcgg agtgacacga ggtgaatgtc caaggactac
    15301 gattgttcct gattgtacca attgggactc tgcaagcttt tccagcgtgg agatgatctc
    15361 cggatccgca tagggtgggt ccatcacgat gaggtcgtag gcacggttgg accgcttaat
    15421 gaacgtcgtg acagacatct ggtgcacgtg acctgcgcgg tcaaatccgg atgtgcggag
    15481 attgtcacgg ataaccgcgc acggctcacg gcccatgtcc acgaagtccg catgggaggc
    15541 tccgcgtgag agcgcctcga tcccgatggc gccggttccc gcaaaaagat ccagcacact
    15601 gtcgggatcg acgccaaggg aggcgaggga gttgaagacc gcacccttga tcttgtcggt
    15661 catcggccgg gtcgcgtgcg agggcggtcc cttcagcctg cggccgcgtg ccgaacccga
    15721 tatgatgcgc atctcgtctg ttccccaatc cccgcttgaa acgatgttgc agggatgcta
    15781 cagggtttgc ctggaatggc gccactccgt gccgcccgta cacccttgcg gagcgtgcta
    15841 cggggacact gttacccctt accaaaagtt caggtatctg cattggcagg atcgcgggtc
    15901 aggcagaggt gtgtgcccag tggcccgcgt tcgcttagcg cttgatcgtg tgactgacac
    15961 acgcgttgtc tccctgtggg acagggttag gacaatcggc tgtctggctc ctggcgatgc
    16021 gcccaaaagc gctggaggcg cggcttaagg cgttggaaac gtgccacatc gatattggga
    16081 cttgcttcga tcagtcgttc ggcgcactcc ctcgcaagat cgaggatgcg tgtatcgaag
    16141 ccctgatcca gccagcccag ttcgggcaga ccgctttggc gggtgccgag gaaatcaccg
    16201 ggaccacgga gctcgaggtc ccgttcggcg agcacaaaac catcgtccga ggcgaccatt
    16261 gtgtgcaggc gggcgcctcc atccattgaa acctcgtcgg cgagcagaag gcagtaactc
    16321 ttgtttccac cgcgaccaac tcggccacgg aactggtgca gctgggccag gccgaaccgg
    16381 tcagcgccct cgatcatcat cacagtggcg ttcgggatat cgataccaac ctcgatgacc
    16441 gatgtggaga cgagaatgtc gaattcgcgg tcccggaagc gcgacatgac ctcgtccttt
    16501 atcctgccgg acatttttcc gtgaaggaca tcgacgcgaa gatcaggaaa aacttcttcc
    16561 tggaggcgct gtgcttcgtc gacggcggcc ttcgcctcga tcatgtcgga ttcttcgacc
    16621 agcgggcaga tgacgaagac ctgatgtcct cctgcaactt cctccctcac gatctggtac
    16681 gcttgtgcgc gatcctgagc ggtataacgg atcgtctcga tgggaatacg tccgggaggt
    16741 cgctcgtcta tgatcgagac atccaagtcg ccatggagca ccagattcag cgaccgcggg
    16801 atcggtgtcg ccgtcatcga caggacatgg ggctggaagc ccggtgcttt cgtcgtgagg
    16861 agtccccgct gccgcacccc gaaacgatgt tgctcgtcga tcacgacgag tccaagagat
    16921 tggaacgtga cgtcatcctg aattagcgca tgggttccga taaggagatc gatctgcccg
    16981 tccacaagcg ccgccagaat ctcgcggcgt tgggcagtcc gagtcgagcc ggtgagcaag
    17041 gcaacgcgcg gccgtaattc ttcgggaagt ggcgcaaaaa gagactccag gctcgccgca
    17101 tgctgctccg ccagcaattc cgtcggtgcc atgatggcgc actgcatttg attcccgcag
    17161 gcaacgaggg caacggaagc ggcgacgacc gtcttgccgg aaccgacatc gccttgcagc
    17221 aaccgggtca tcggctggtc cttgcggatg tcggtaagga tctcgccgac ggcacgagtc
    17281 tgggcacctg tgagatggaa gggcagggta gagtagaagc cgtccagggt ggatttctcc
    17341 acggagagcg cgcgcccttt ctcctgctta cgtcgctttt tctgctgcac cagaccgagt
    17401 tggagcagga aaaggttctc gaacatcaga cgcctgcgtg cgcgctcgaa gtcctcacgc
    17461 attggcggat agtgaagatg ctcgtacatc tggtccagcg gtggcagggc attccagacg
    17521 ttctcgggga aaaattcacg cgcgtcggcc aaccagtcca ccagctgctt cttcgtcgcg
    17581 tccagtacgt tacgcgtcag cttgcggagc gatttctgcg tgacgccctt ggtgagcgag
    17641 tagactggaa tcaaccctcc cgtcgagagt ccttcgctgc caacgcgctc ccactcgggg
    17701 ttggtgatct gtagtccccc gtatccgggc atgactgccc cactgacgac aatctcgtcg
    17761 ccctcagcga gttgcttggt gatataggtg ctgaaccaag tgatgcgcag tgaaccggtt
    17821 ccatcgaaga gccgcgcttg cactcgtggg gtgcccaccc ggatctcccg catttctacg
    17881 agtttgcccc gaacggtaat ctctccacgg agacccagcg gatcatcgag ccggacggtg
    17941 tgggagtaat cgatatgccg gcgtggcacc atccggagga cgtcttcaag ggtgtggagc
    18001 tgcagattat cttcgtaggg cttgatcctc ttcgggccga atccaggcag gtcgctaagg
    18061 agtgtttcag gcggttgctg cccgatcgtc gccacgcggc gtggcgctgg ttccctaatg
    18121 ggatggccta cgcgtttctc tcccaacgcg ctgttgagct tgcccaactc tctcggtgat
    18181 tcgttctgtc ccaggtgtgg cttgattgct tgtagacgtt tgccaaggag ttcgacctcc
    18241 tggcggcgtg ccgattcctc gcgggaaaga tgcatgagac tgtcgatggt ggattcgagg
    18301 gtggcagcaa tctcgtgtgt gctggcaatc tggtgtgtca gatggcgaag ggcatcgacg
    18361 gcgatggcaa tatcaggacc aagatccggc cctcctaccc acacccggcg tagcgcatcg
    18421 atcgcgcgct cgaggtactg gacgcgagag gtcgtgcttg gtcgtgacgg tagacgggga
    18481 gctctcgaag gaggcaatac cggatcctcg atagacatgc gctggtgtgg acatacccgc
    18541 ccgcgcacct tcctgcacgg gcggtacgtg ttcagtctag ctgaatgacg agggatggtt
    18601 agggacgctt gccaagcgtc aggatgccaa tggcccccgg ccctgcatag gtgaggattg
    18661 tggacccggt ccaatcgatg atgatacttg cgtcagggag gcgttgccgg agagcatccg
    18721 cagtggtctc cgcgtccttc aggttgtcac tgtggagaac tgcgatgtcc tgtggatcac
    18781 ccgttgtggc ggccagctca actgcgcgtg caagcgcctt cttccacgtc cgtacccgct
    18841 cgatgggtgt gaggatgccg tcgacgaagt tcaatactgg tttgatcccc agagccgatc
    18901 ccacgatatg cgatgcacgt cctatgcggc cacccttata gacgtagtcc agcgtctgca
    18961 ataccgcaaa gcagttggcg cgcccgatcg cctccgtggc acggagtgcc gcctcgtcaa
    19021 ggccagcgcc actctggact gcacgggcgg cctcgatcac gatccaaccg agctgcatgg
    19081 tggtggagcg cgaatcgacg acccgaatgc gttcagagtc gaattcttct gctgcaaggc
    19141 gggcggaatt gtacgtaccc gagagctggg acgaaagtgt gatgcacagg acgtccattc
    19201 ccctgtcgat ggcctcgtta aaaaccgagg caaatcgcgt cgaggggggc tgcgaggtct
    19261 ttgggagatc cttctcttgc gtcaggcgtt ggagatattg cggaggtgtg atgtcgacac
    19321 cgtcctggaa cgtatcgctg ccaaacgtga cgttgagggg cacgaccgtg atgtcatact
    19381 gatctcgcag cgaaccggga atatccgacg tggaatcggt gacaatgaga acctcgcgcg
    19441 tctcagtcac tcaatctctc cttgacgatg ataccgagga catccgggcc aatatgcgtg
    19501 gcgatcaccg gaccgagctg gacaatgatg acgtcgtgct ccggcgtcag cgttgccacc
    19561 ttgtccgcga gcttccgggc atcgccgggc gtcgtgttat agatcacggc gatctcttca
    19621 ggcacgccta cggacccggc aaatgcggcg agagcatcca ttgccttgcc ccgggttcgg
    19681 gtgcgttcga aaggaaccac ctggccttca tcgatgcgta gcagcggccg aagctgcaaa
    19741 agagagccga ccaattgtgc ggccttgccg atacggccac cgcgacggag atgctcaagc
    19801 gtctccacaa agaacacaac atggtagtgg cccagctcag agcgcagggt cgatgcgatc
    19861 gttgtagcat cctgaccgtt cgaggccagc tccgccgcgc gtagcacctg gaagccaagg
    19921 ccgtaggtga cattgagcgt gtccaccagt tccaccttga tcgtctcggc aacattcatc
    19981 gccgcgatct gggccgactg gtatgtgccg ctcagtttcg acgagagcac gacgcagacg
    20041 atctcgctgt gcgtttcggc tacttcacga aacgccgttt cgaacgcacc gattgagggc
    20101 tgtgaggtcg tcggcaaagt gtttgccgct gccatacggg ccataaactc gtcagttgta
    20161 atgtcgatct ggtcgcggaa aacctcatcg ccgaagtgga cgttgagcgg cacaatcgtg
    20221 atgtcgtgcg cggcggcgac ttcgaatgag agatcacttg tgctgtccgt gacgacggca
    20281 actgatgatc gttgcgacat ggtgctcagg tctcccctcc cggtcagtat ccgtgcgcct
    20341 gcgaggtgtt ctcttgaaga caggtactgg atttcgggtc agcataccgc agagcaacgg
    20401 tatttccccg agggggctga ggcccggaac gttcagtagc tcgaatggcc cgcgggtaca
    20461 tggcgactac tcgacggcaa tgaggtatcg atagtgaggc tggttcccct cgactatctc
    20521 gatctccgcc tccggaaaaa cctcttccgc agtcgcccta agtgcctcgg tgccgggctc
    20581 cgtagcgtct tcgccgacaa agactgtcag gagctcgggt tcactgtcct gaagcgaggt
    20641 caatacactc tccaggatct gctggagatc aggacccgat gccacgagat caccatccac
    20701 cagcccgatc agatgcccct tcacaacggt aacaccgttc aactcgacgt cgcggacggc
    20761 ctgcgtaatc tcgatggagt gcacatcctc tagcgcgctg ctcataacct gctcgttgac
    20821 gttcagcgac gcgtcccggt tgaacgcagc cagggccgag agcccctggg gcaatgacct
    20881 ggatggaaca accctgaccg tcttttccgt gagaccggca acctggttcg cggccatgac
    20941 gatgttcttg ttgttcggaa ggatgataat ctgatccacc ggtagggcct cgacggcttg
    21001 cagaagatcc tccgtgctcg ggttcatcgt ctggccgcca cgcacaatgc agtccgctcc
    21061 catcgatcga agtgccgctg ccaacccatc tcctgctgcg accgcgatga cgccgatgtc
    21121 gccatcgatc gactgggcaa cgtccgggtt ggatgcatga gcagggacag ccttgtcacg
    21181 ggcttgttgt cgctgatcgg tgagagccga ggtctggagt gtcatattgt cgatcttgat
    21241 ctgatcgaga tatcccagcg tcagggcgta ctcgagcacg ctgcctggat tctcagtgtg
    21301 aatgtgtacc ttgacgacgg tgtcgtcacc gacgaccacc acagactggc ccattgcggc
    21361 gatttccgaa cgcacggtac cgaagtcgat cccttcaccg aagaccatga agttggtgca
    21421 gtagccgaac gggtcctcgc catgggtttc cgtgacgtcg tcaaggaacc gcatatcggc
    21481 gccaatgcca gccgctgaga gatcttccac gagcgccgtg tcgccctggg tgtagcgata
    21541 gagaccctcg aggatgtgaa ccacgccctg cccgccggca tcgacgacgc cagctcggcg
    21601 gagaatctcg agcatatcag gggtttgggc gagcgcctcg ttcgctgcgt ccagtgagtc
    21661 tttcaggact gctcccagtg atggcgtacg gtgggccgtg gtcccggcac gctccgccgc
    21721 aacccggata atcgtgagca tggtgccttc gacaggtcgc atcacggcct tgtacgccat
    21781 ctcacgcgcg ccttcgagtg ctttcgccag gtcatggcca tcaatcgtgt cgcggtccgt
    21841 tattgccgca ccgaaccccc tgaagatctg ggaaagaatg acccccgagt ttcccctggc
    21901 gcccatcaac gaaccgaagg cgaacttcga cgcgacactg cttgcgctgg aaggattgtc
    21961 ggcatttcgg gcggcgtcga gcgaagcgtg catggtaagc gccatgtttg tcccggtgtc
    22021 accgtcgggg attgggaaga cgttgagcgc gttcactcga tcacggttgt ggtcgagcca
    22081 tttggtcgcg aagtccaggg catcgagaag ttcgtcgccg ttccacatat cactcaggga
    22141 ggtgccgcgg atgggttgct gcatggttat actgcgctcc cggacgggga gcttacccgc
    22201 aggccgtcga cattgacgtt gacgcgatcc acatccatac caagcatacg ctcgacctgg
    22261 aacttcaccg tctgcattac gttctgggcg actgtgaaga taggtgtgcc atattcgaca
    22321 accacagaaa gctcaatggt gagatggcca tcttcattct ggatgccaat gccacgcggg
    22381 ccgttgccaa tgcccaggcg tttgacgatg gcggaaccca gcgagcgcgg gcccatgtcc
    22441 acgactccgt aacacgagag aacagtctcg tgaatgatgc cgataatcgc gctctcggcg
    22501 atctcaactc gcccggcggg agctgaatcc tgcaatggct cgtgatgctc cacggttgag
    22561 gctcctgatc atatgacggt ttttgaacgg tgtgcggaaa aggacacgca gcctgagtgt
    22621 ccggcagtaa gtatgatgat acctaaaaac ccggtggcgc ggcccggagg cgtgtgatag
    22681 catatggact tggcgagcgc gtcgtcctcc cgacggcgct gctttcacgt ccaaatgggt
    22741 ttgggcgtta tgacttgtga tatttcatag catcttgcgt gtgatcgggc gttcgataca
    22801 tcccctgtcg ctgcatttga gcaatctttt gaagggagca tgcccgtggc cgggaaatgt
    22861 gatgtctgtg ggaaagccaa gacgttcggc aataatgtga gcttctccaa gcgccgtacc
    22921 aatcgtgatt ttcgcccgaa tgtgcacaag aagcgcatta ttgttaatgg tgtcgggatt
    22981 cgtctgaaca tctgcacacg gtgccagcgc acgcttggca aggctggcaa gacgaaggtt
    23041 gcgtcctagc ctcttacctc cagacaacat gcatgaggca cgcggtgggt tatcccgccg
    23101 ggtgttttgc tatgtcactt gctcgagtgc cagggcgatt tcgtcctgta ccgacgtgtc
    23161 gggctcaagg gttctcgctc gatccagcgt ggtccgcgcc accgggccgc caatgcggcc
    23221 cagcgcccag gcagcatggc cgcggacgat cggctcgtca tgtgagatag cagcaccggc
    23281 cagagtaggc acatcgtccc tcgtcccgat gttgccgagc gctaccgcgg cattccgggc
    23341 gagaccgcgt cgcttggtgc gcgggacggg agtaccgaag tacgtttctc cgaattcatc
    23401 ctgggtcatc gtcagcagcc aatgcagcga ggggaacgca ttgttgagcg accgcggttg
    23461 catatcagga tcattgacga tctccgctgc gccggtgtag gggcaaacct cctggcagac
    23521 atcgcagcca tacacccagt cgcccatctg cggccggagc tggtacggta tcgcgtctcg
    23581 cagctcaatc gtgagatagg agatgcatga cggcgcgtgg atcgtataag gctccacgat
    23641 ggcgccggtt gggcagttgt cgatacagat ggtgcagcga ccgcagtttc ggtccaacgg
    23701 ctggtccggc tccaggtcca ggtcggtgac catctcgcca aggagtaccc aggacccgcg
    23761 tcccgggacg atcaggttgg cgttcttgcc gtaccagccg agaccggcgc gcgcagcgac
    23821 agcgcgctcg acgacgcgag cggtatcgac gaggatcctg gcatctagct cgcgaccggc
    23881 aagttcgttc aggtcggata gcagcgcctt catccggcgc ttgagtaccc ggtggtagtc
    23941 cttaccccag gcatagcggg agattttgcc gcgtggcact ccatctacag gcttttcggc
    24001 tggtccggac cagtaggcga gcccgaagga gatgatcgac tgtgcggtcg gatgcaggtt
    24061 gcgaatatcc gaggagaccc gcgcgcgctc ctgcgtgaac cagtccatgc cctcaacgtg
    24121 gcccgcttca atatgcgctt caagctggct ggcgacggca gggaagggat cggcagtcgt
    24181 taccgcactg accgccagtc cgtgcctcgc cgcgaggtac ttcacctgcg cggtgagccg
    24241 ctggcgatcg gtggtgggag cagtatggag tgtgagtggc gaagcagcga catccatgag
    24301 gtgagtatat ccggcgctct acttcgtaat ctgcgaaggg tccgcagcgg cggttgcggc
    24361 gatatcccag tcagcgcggt gggcaagtcc ccaggcgatg cctgccacgg cttgggggag
    24421 caacaggtcg gggacaacgt gttgaattgg acggagcacg cggtcttccc gggcgatgag
    24481 ctccagtccg tccggtgtga ccgcggcggc ggcgcggagg tgggttcctg gcatgcgatc
    24541 gaagatgtcg ccgagggcgt ccgttggcga tgggcgtccg gtcagcttcg cgtattgatt
    24601 ggcagtgccc acgatggtat cgaagcgcag ggcgatctcc atctcatcga tgctcggctc
    24661 attccagtcg agatgtccca gcgttccaat catccgcaca ttgccgtcag tgtgcgcggg
    24721 aaggtcggaa aggaagcggc gaagtctctg gtcgcgggcg tccagcacca gggcatcatg
    24781 gccaaagagg tcgtagatgt ccatgatcgc acccttttcg ataccaatac cgtccctgcg
    24841 ctcgataacc tcaccatcat cattccgacg aacgatggct gtcgtcgctc catccgtctt
    24901 gtcgagcaac cccggatgga tacgagcttg tttcaggatg ctgagcacct cgttgccagg
    24961 gtcgtcggta gcaagcaccg atcgcagttt gatcgatcca ccaagcatgg cgatagcgag
    25021 cgcaattgct gcggaaccgc cgggacggag ggtagtattg tgcgcttcag cgatgatgtc
    25081 accaataacc gcatagcgtt ttgtcatgga atgctcgtca gaaccgcgcg agttggcgca
    25141 ccatcaccac acatcagacg catcggcagg caggtcagct gatagtatcc gggctcgact
    25201 gcccggagat cgagcccttc aaggatcaat acattactac tgagcagtgt gatatgggta
    25261 gcgtggtgcg gttcatcgaa gggggcgatg ccgagatagt cgatgccaac aagctgaata
    25321 ccccggtcga cgatccaccc agcggccgcg gcgtcgatac caacgaacgc gggatcgaac
    25381 gaccacgggc gaggacggtc ccaggagctg gagtttcgcg ttttcagcag tgggtgagtg
    25441 acgcccccgg gaatgtcggc ttgctccaga tcggaagctg taacgacatc gacagtttcg
    25501 gggatgcgag cgacgtagca cctgcccgac cagcgtgcga tcggaatctc ctcgagacgt
    25561 tgcccgtcct ccagatagtg ccagtgcgca tcaacatggg tgcccgtatg ggtaccaaga
    25621 gaaacgacgc tcgtgttgaa ttcattgtgg gcgatcgtcg cgtccggttc gagagtaaca
    25681 tcgggatctc ccggctatgt gagggagccg gtctcgatgg gcaatgtaat gtcgtagacg
    25741 cgtggtgtgc tcatagtctc taccggttcc ctccgatcct tccgacgatc ctgtaatgcc
    25801 gcctcaggta cgctcccagt aggcaaggat acgttgaggt ggcgtattct tggggcgtgg
    25861 agtcgaagtg gaaggttcca tttcccgcta tcgatcgtga ggacggatgg ttgatccggt
    25921 atggtgacga tcggaggaag gttcccgtgt ctttaccctt ggcccggcgt ccattatccg
    25981 cggcacgccg ccggcaacga aacgagcggt gtgcagcgat tttggaggca gcagggcggt
    26041 tgatcgcccg tgacgggttg tcgggccttt cgatgcgtct cttggcgaaa gagctcgggg
    26101 taccggcgcc gactctctac gggtattttg attcaaagga agccgtgatt gatgcgctgg
    26161 cgcaggagaa aatcgcactc cttcgggagg caatacttag cgaggcgaaa gatgcctcgc
    26221 cgggagtgcc ccgcctgctt gcattcgcgc gcggataccg gacattcgcc ctcagcggtc
    26281 gcgactacta cgacctcttc atcctgaaag caacaccgga cgggcaagac gcgcggacgg
    26341 tggacaatac ggctggcatg gagttgatcc agacacttgc cgaggacgtc caaacagcaa
    26401 ttgacctggg tcagctgcaa cccattgacc cggagcatac cattctcgca ctgtgggcgg
    26461 tggcacacgg gtacgtctcg ctggaggtgt ccggcgcgct ggacgacctt gattccagtc
    26521 cggtctctcg cgagcgaacc tatctgctct attttgaatc gatgctgcgc ggtctcgagg
    26581 tatgcgcacc gccacagccg acttccagcg aatgacctga atcaccccgt accggcattg
    26641 agggcacatg tcatggcgag acctcggacg acgaagagga ccacaccgac tgcaggatcg
    26701 ctcctgggag agtgggtgcg tcgcgcgcgg caggagcagg ggctgagcca acgggcgttg
    26761 gcggatcagt ccggcctgag tcgttcatat gtttgtgaca tcgaacgggg acgtggcgcc
    26821 gagccgagcc tgacgacgct ggacaagttg gcggcggcgc ttggcgcctc acgggccgac
    26881 ctgatgcggg cagcggggct gatcgaccgt gcactggtgc cacgcgagtc cgaggaggaa
    26941 cggcgtctct tgtccgttta ccgggatctg agcgacgagg ggcagttgaa cattttgcgg
    27001 tttgcgcgat tcctgcatca ggaagagcac gcgtggacgc agatttcact ggtaggtgac
    27061 aacggggcag aggagagcgc cccgccgcca acaccggatg cgcgaaggcc ggcactcttt
    27121 tcactcgacg acgggaacgg gtagcaccat ttaccccgcg ccaggctatg ggccggaatc
    27181 tcatgacaag catcatcaat gcagcacgac aaggcgacta gtccttcccg cttcagttcg
    27241 tgctccccgc tacgtcgctc gacaagcaaa agcccgggtt tcattgtgaa tacccggggc
    27301 ttttgctgtt ctggtatcgg gagcggcgag gaattacttc tccgaatcag ctgcttcctc
    27361 ggcgaggacg ttctcaggcg aggcatcagt ggaggtctgg cctgcagaag cggcctggac
    27421 ctgccgggct tcccgctcgg ccttggcggc ttccgcggcc tcgcgctcgg cgcgagcagt
    27481 ctcggcagct tcgcgctcag cacggcgctt ggcacgctct tccttggcag cagcctctct
    27541 ctgtgcggcg gcctcagcct cggcctcggc tcgggtacga gccagctcct cggcgcgacg
    27601 ctggcgcttg gacttcacgc cacttgtgga ctgcgcggcc tccatgcgct tggtaaagcg
    27661 ctcgacctga ccggccgtgt caacaatgcg ctgctcgccc gtgtagaacg ggtggcaaac
    27721 gttgcagagg tctgtgcgga ggttcgactt cgtcgaccgc gtcgtgaagg tgttgccaca
    27781 cgagcagacg actgtggctt cttggtagtt cgggtgaatt cccggcttca tgtgtatggt
    27841 tctccgtggg ttctaagcca ggttactgcc taatcggcag cggtgacttc ggccgcagct
    27901 ggggccgacg tgagctcggt ggcgccgccg accgggtaga tctccggcgg gtagacgctg
    27961 atgcgacgct tttgacgcga gatggtctcg aagcggacaa caccgtcgat cagcgaatag
    28021 atcgtatgat ccttgccaat accgacgttg ttgccgaccc aatactgggt ccctcgctgg
    28081 cggacaacaa tggtcccggt gcggacgtac tgaccatcgc cccgtttgac accaagccgc
    28141 ttggactcgc tgtcgcggcc attacgggta ctgcccatac ctttcttgtg agccatgatg
    28201 ttgtgtcctc tctcacagcg gtgcgtcgtg aaaacgccac tcgccggtaa tgtgctagcc
    28261 ggtgatgcca gtgatggtca cccgggtgag ttcctgtcgg tggcccatct ttcgacgata
    28321 gcccttcttg gccttgtact tgaagaccac gatcttctcg ccgcgcttgt gctcgtcgat
    28381 cgtcgcagac accgtcgcgc cctcgacaac cggggtgccg atccgcgtgg aaccgtcgcc
    28441 acccagcatc agcacgcgat cgaggataat ctcgctgccc gcatcgtcgg ctaggcgctc
    28501 aatcgtaatg tgatcaccta ctgacacgcg gtactgcttg ccacccgtct ccactactgc
    28561 atagggggag agctccggcg cggttttaga cttctttttg gagggagcgg tcgccggggc
    28621 agaatcttca gtagactctg cc
//
LOCUS       DASYWM010000105         4483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_134961, whole genome shotgun sequence.
ACCESSION   DASYWM010000105 DASYWM010000000
VERSION     DASYWM010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134961"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VGR29_04430"
     CDS             complement(<1..558)
                     /locus_tag="VGR29_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072871.1"
                     /translation="MHPDRQMLASLLSLQLNRRTTLIGAATAVGAASATTHVVAQDGT
                     PQVSVDATPEGQPGGVLRVGVQGDPTELDPHLVVLDAASLITDLVYEGLVHEDPSLVP
                     QPLLAKSWEISEDGTIYTFALRQGVTFHNGRALVADDVVYSIERVMDPETASPWVSYT
                     DTIQKVEAPDQKTVVFTLSAPDASFL"
     gene            complement(684..2324)
                     /locus_tag="VGR29_04435"
     CDS             complement(684..2324)
                     /locus_tag="VGR29_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2072872.1"
                     /translation="MYQPVQKRSQAAVVESLAHDVLTGRLTRRDAIVRGAALGLSVPA
                     MMALGAVGNAPLRASAQEASPVARTGGTLRVGLSADPAELDPHKTSLTAAWHVLEHVY
                     EGLVTTNAALEPVPVLAEEWEISEDGLTYTFKLRQGVTFHNGREFVADDVKYSFERIL
                     DPETASPNVSELGSIETIEVVDPSTVVITLSAPDSSFLAKLMGTSIAIVPQEEVEANG
                     DLMQVAVGTGPFQFVEYVPNSVVTLERYPDYWEEGKPYLDGLEMQIVPDNTSRTTALV
                     TGTVDFIEYAPVQDLPIFESDETIKVAGDQNTNIRYMAINVSREPFTDVRVRQAIAMV
                     IDRGPIIDSAVFGAGTATNILFPESYWAGFPSEIPEPDIEGAKQLLAEAGYPDGFKTS
                     ILSWAQYAFLSNAAIVIQEQLKQIGIEAELELQENAIYLENYFAGNFDLSVTGTSAYV
                     DPNDVVLANFGTGESNNGIGYSNPEMDELIAQAMEETDQAERARIYRRIQEIILEDVV
                     WVNLFIANQYEAMKEYVMGYEHIPTGSNRSFQDVWLDQ"
     gene            2621..3934
                     /locus_tag="VGR29_04440"
     CDS             2621..3934
                     /locus_tag="VGR29_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEV2072873.1"
                     /translation="MSSITPLNVIYLHSHDTGRYVEPYGYAVKTPRIQRLAEEGVLFR
                     QAFSAAPTCSPSRAALLTGETPHQAGMIGLAHRGFCLADPSRHLAHRLKDAGYRTVLA
                     GMQHLTEGDQAQLGYTDDLRPTSLAVADVAPIAASVIEAHAGDGAPLFLDVGFEETHR
                     PFHGANSTDPRYVLPPDPLPNTSETRRDMADYLGSARELDRGVGIVLDAIGRASLADS
                     TLVICTTDHGLAFPGMKSNLTDHGIGVMLIMRGQQGFTGGRVVDALVSHLDLYPTLCE
                     MLSFPCPTWVQGRSLLPLIHGEQSQIRDEIHAEVTFHAAYEPQRAVRTSRWLYIRRFG
                     AHEFPVLANVNESPSRDLWLEHNWEHRHIDAEQLYDLILDPIQRCNLFGTPEVALVQS
                     ELQARLERWMRETDDPLLRGDVPLPRGALMNDPKARSADEPLIMG"
     gene            4103..>4483
                     /locus_tag="VGR29_04445"
     CDS             4103..>4483
                     /locus_tag="VGR29_04445"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEV2072874.1"
                     /translation="MQITGEQKIAALREQVYDAMLDPEVLKGALPGCEKLEEVGENEY
                     IATMTIGVAMIKGKYDGKVKITDQNRPESFTMHIEGKGPQGQLGGEGKLNFEDDGEGG
                     TIVKYAGDANVRGTLARIGSRVIQP"
BASE COUNT          930 a         1360 c         1342 g          851 t
ORIGIN      
        1 gagaaacgac gcgtctgggg cagacagggt aaagacgacg gtcttctggt caggcgcctc
       61 cactttctgg atggtatcgg tgtagctcac ccagggagag gctgtctccg gatccatgac
      121 tcgctcgatg ctgtagacaa cgtcatccgc caccagcgcg cgaccattgt gaaaggtcac
      181 cccctgacga agcgcgaagg tgtagatggt gccatcctcc gagatctccc aggatttggc
      241 cagcaacggc tgcggcacca acgagggatc ctcatgcacg aggccctcat agacgagatc
      301 ggtgatcagc gacgcggcgt ccagcaccac cagatgggga tcgagctccg tcggatcgcc
      361 ctggacgcca acacgaagaa caccaccagg ctgcccctcg ggagtggcat cgactgacac
      421 ctgaggggtt ccgtcctgag cgacgacgtg agtggttgcg ctagcagcgc caacagccgt
      481 ggccgctccg atcagagtgg tacgacgatt cagctgtaat gaaagaaggg aggcgagcat
      541 ctgccgatca gggtgcatca agttctcttt ccgtagagca taccggttcc gaatgttgcc
      601 taatacttta ctacggaaag catagcgatc gcagggctcc tgacggatgg tctcggcacg
      661 ccgggaccat ccgtcgaacc gtgctactgg tcgagccaaa cgtcctggaa ggacctgttc
      721 gatccagtcg ggatgtgctc atagcccatc acgtactcct tcatcgcctc gtactgattg
      781 gcaatgaaga ggttgaccca gacgacatcc tcgagaatga tctcctggat ccgcctgtag
      841 atcctggccc gctccgcctg gtctgtctcc tccatcgcct gggcaatcag ctcatccatt
      901 tccggattgc tgtagccaat gccgttattc gactcaccgg tgccgaagtt ggccagcacg
      961 acatcgttcg ggtcgacata ggcactggtg ccggtgacag agagatcgaa gttcccggcg
     1021 aagtagttct ccagatagat cgcgttctcc tggagctcaa gttccgcctc aatcccgatc
     1081 tgcttcagtt gctcctggat aacgatggcg gcattcgaga ggaatgcgta ctgcgcccag
     1141 gagaggatgc tcgtcttgaa accgtccggg tatccggctt cggccagcag ttgcttcgcg
     1201 ccctcgatgt ccggctcagg tatctcggag gggaacccag cccagtagga ttcggggaag
     1261 agaatgttcg tggccgtacc ggcgccaaac accgccgaat cgatgatggg accgcggtcg
     1321 atcaccatgg cgatggcctg gcgcaccctc acatcggtga acggctcgcg ggagacattg
     1381 atcgccatgt agcggatatt ggtgttctgg tcgccggcta ccttgatcgt ctcgtcactc
     1441 tcgaagatcg gcagatcctg cactggtgcg tactcgatga aatcgacggt acccgtcacc
     1501 agcgcggttg tgcgggaggt attatccggc acgatctgca tttccagacc atccaggtaa
     1561 ggcttgccct cttcccagta atccgggtac cgctccagcg tgacaacgga gttgggaacg
     1621 tactcaacaa actggaacgg gcccgtgcca accgccacct gcatcaggtc gccgtttgcc
     1681 tcgacctcct cctgcggcac gatggcaatg gaggtcccca tcagcttggc gaggaatgag
     1741 gagtccggcg cactgagcgt aatgaccacg gtcgaaggat ccacaacctc gatcgtttcg
     1801 atcgagccga gctcggagac gtttggcgaa gccgtttcgg ggtcgagaat acgctcgaag
     1861 ctgtacttca catcgtcggc aacgaactcg cggccattgt gaaaggtgac accctggcgt
     1921 agcttgaagg tgtaggtcag accgtcctcg gagatctccc actcctcggc caggaccggc
     1981 accggctcca gcgctgcgtt cgtcgttacg agtccctcgt aaacgtgctc aagcacgtgc
     2041 caggcggcag tgagggaggt tttgtgagga tccagctcgg ccggatccgc cgagagacct
     2101 acgcgcagcg tgccaccggt cctggctacc ggcgatgcct cctgagcact tgcccggagt
     2161 ggagcgttgc ctacggcacc aagcgccatc atcgcgggaa cggaaagacc aagggcagcg
     2221 ccgcggacaa tcgcatcgcg ccgagtcaga cgtccggtaa ggacatcatg cgcgagcgac
     2281 tccaccactg ctgcctggga acgtttctgg actggttggt acatttacga ggttctcctt
     2341 gttctgcgaa acgtcatctc gaggacccac gaaacctgca ctgccggcag gctcacgtac
     2401 tttcaccgcc tgacaggcat cgccggcgcc ctgatcgtca aaacacgagc aagggatgtc
     2461 acgcgccatt gcgcgaccag gttttccgcc agttggcagg aagtaacgtg agtatacggg
     2521 caccccgggt ccggcacaac gtaaagcggt gcctcggtcc agattgtgcg acgggcgaga
     2581 tggtgaggtt ccggatgttc gtatatcagg agcggcgtgc atgtcttcga tcacccctct
     2641 aaacgtcatc tatctgcact cacacgacac tggtcgctat gtggagccct acggatatgc
     2701 cgtcaaaact ccgcgtatcc aacgtctcgc tgaggagggg gtgctcttcc gtcaagcctt
     2761 ctctgctgcc ccaacctgct ccccgagccg ggcagcgctc ctgacaggag agacaccgca
     2821 ccaggcgggt atgatcggtc tcgcgcatcg cggcttctgc ctcgccgacc cgtcacgcca
     2881 cctggcacac cggctcaagg atgccggtta ccgtaccgtg ctcgccggaa tgcagcacct
     2941 gacggaggga gatcaggcgc agctggggta caccgatgat ctccgcccta cgtcgctggc
     3001 ggttgccgac gtcgctccga ttgccgcctc cgtcatcgag gctcacgccg gcgatggtgc
     3061 gcccctcttt ctggatgtcg gcttcgagga gacccaccgc cccttccacg gcgcgaacag
     3121 cacggatccg agatacgtcc tccctcccga cccgctcccg aatacctcgg agacgcgaag
     3181 ggacatggcg gattacctcg ggagcgcacg cgaactcgac cgaggggttg gcatcgtgct
     3241 ggatgcgatc ggccgcgcga gtctggcaga ttccacgctg gtcatctgca cgaccgatca
     3301 cggccttgcc ttccccggca tgaaatccaa cctgacggat cacggcatcg gcgtgatgct
     3361 catcatgcgg ggtcagcaag gtttcacagg gggacgagtt gttgatgcgc tggtctccca
     3421 ccttgatctc tacccgacac tctgcgagat gctgagtttc ccctgcccga catgggtgca
     3481 gggcagatcg ctactcccac tgatccacgg cgagcaatcg cagatccggg acgagattca
     3541 tgctgaggta acgttccacg ccgcatacga gccgcaacgt gcagtgcgaa cctctaggtg
     3601 gctctacatc cgtcgctttg gtgctcatga gttccccgtc ctggccaatg tgaacgagag
     3661 ccccagccgt gacctctggc tggaacacaa ctgggagcac cgtcacattg atgccgagca
     3721 actctacgac ctgatcctgg atccgataca acggtgtaat ctcttcggca cccctgaggt
     3781 ggccctggtc cagagcgagc ttcaggcgcg cctcgagcgg tggatgcggg agacagatga
     3841 tccgctcctg cgtggagacg taccgctccc tcgcggcgca ttgatgaatg acccgaaagc
     3901 ccgcagcgcg gatgagccgc tcattatggg ctgacggaaa agtccggagt gggtcgtgca
     3961 agtgcacatt tcatggggag acatgaagcg cgtatggccc attccctcca tcggaatgac
     4021 aatagaggaa cgacatagag gaaatgcttt cgcaagcatc gtgtatcgga tagcataccg
     4081 gcagaaaagg agagataggt tcatgcagat tacgggtgaa cagaagattg ctgctctgcg
     4141 ggagcaggtc tatgacgcga tgctcgaccc ggaggtactg aagggtgccc tccccggttg
     4201 cgagaagctc gaggaagtgg gagagaacga gtacatcgcg accatgacga tcggcgtcgc
     4261 gatgatcaag ggtaaatacg acggcaaggt caagatcacc gaccagaacc ggcccgaaag
     4321 cttcacgatg cacatcgagg gcaagggccc gcagggacaa ctcggcggcg aaggcaagct
     4381 caacttcgag gatgatggcg aaggcggcac gatcgtcaaa tatgcaggcg atgccaatgt
     4441 ccggggcaca cttgcccgca ttggcagccg cgtcatccag cca
//
LOCUS       DASYWM010000106         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_172488, whole genome shotgun sequence.
ACCESSION   DASYWM010000106 DASYWM010000000
VERSION     DASYWM010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172488"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VGR29_04450"
     CDS             complement(<1..215)
                     /locus_tag="VGR29_04450"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEV2072875.1"
                     /translation="MSDICVGLISGTSLDGIDVALCRIDGHAQEAAIELLRFETVPYP
                     KAVRDELFALYEDQRNAVARLCAMNVVV"
     gene            complement(212..1387)
                     /locus_tag="VGR29_04455"
     CDS             complement(212..1387)
                     /locus_tag="VGR29_04455"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV2072876.1"
                     /translation="MTFKTTSPVDAGMDAAQLDEAWDSLEQAAEAHEFGGAVSLIVRH
                     GQIVVARATGWAVREPEEERSPMEMDTILDLASLTKVVATTPAVLKLVADGKIDLDEP
                     VGQILPEFGTEGKKAEVTIRRLMSHSAGMIAWRGVFMEGVGPEAYLESFARDQPEMPP
                     GEQVVYSCAGFITLGEVVRRVTGESVASFATREVFAPLGMTDTMFTPPEHLRDRIAAT
                     EYGNVHETAMAGAAPVGGRWRDYLLRGEVHDGNAWYGFQGVAANAGLFGTAMDLARYG
                     QAWLDGGELDGVRILPEEIVREATREQTGLDAPNDRRGLGWQMAPRPNEDPGNHSGRG
                     LSQRAFGHTGFTGTSLWMDPERDLIIVLLANRVHPTVNPAYLPTRAIFTEAVARACA"
     gene            complement(1478..2704)
                     /locus_tag="VGR29_04460"
     CDS             complement(1478..2704)
                     /locus_tag="VGR29_04460"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV2072877.1"
                     /translation="MSSETPTGPLSGIRVVDLTRVMTGPYGTMMLGDLGADVIKIEQP
                     GKGDDTRHWGPPFVQGEASYFMSVNRNKRSIALDLKSEGGREILWKLIDDADVLVENF
                     SPGTIDRLGFGHEVVLERNPHIVYASISGFGQTGPSRNRTAYDLIVQGMSGMMSITGE
                     VGGMPTKMGIPIADITAGMFVANAVVAALFARERHPEKRGQYVDTSMLGGQIALLTYQ
                     AASWFATGEVPTMSGNRHSIITPYDTFPSADGYVNIAVGNDAIWRRFCDAMGLEDVRD
                     DSRFLTNADRVAHRMELYEIMNRCLSAMTTADLVERLDAAAVPCGPIYGIDEILEDPQ
                     AVDQELVMTAPHKRLGEVQVTGFPYHFHGTPLEVRYGPPVLGEHTREVLAGMGYNDEE
                     IDALIAAGAVEEERQG"
     gene            complement(2851..>3130)
                     /locus_tag="VGR29_04465"
     CDS             complement(2851..>3130)
                     /locus_tag="VGR29_04465"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV2072878.1"
                     /translation="IQTLIFLGIFVTTTMIPAWVMIGYWIVIQVISGVGSLSMIDEQT
                     GGVAFFAHIGGFVAGLVLVWFFRDSRRLEEQREARRGFVEDRRWGMSR"
BASE COUNT          599 a         1101 c          861 g          569 t
ORIGIN      
        1 accacgacgt tcattgcgca gagacgggcg accgcgttcc gctgatcctc gtacagcgcg
       61 aagagttcgt cccgcactgc tttcggatag ggaaccgttt cgaacctgag caattcgatt
      121 gccgcttcct gcgcgtgacc atcgatgcgg cagagggcca cgtcgatacc atcgagcgag
      181 gtaccggaga tcaacccgac gcagatatca ctcatgcgca agccctcgcg accgcctcgg
      241 taaagattgc cctggtaggc agatacgccg ggttcacggt agggtgcacg cggttcgcca
      301 gcagcacgat gatgaggtct cgctccgggt ccatccacag cgacgtcccg gtaaatccgg
      361 tatgcccgaa cgcccgctgc gaaagccctc gccctgaatg attaccggga tcttcattcg
      421 gccgcggcgc catctgccaa ccgaggccac gccggtcatt cggcgcgtca agacccgtct
      481 gctccctcgt cgcctcacgg acgatctcct ctggcagaat ccgcacgcca tcaagctcgc
      541 cgccgtcgag ccaggcttgc ccgtaccggg caagatccat cgccgtgcca aagagcccgg
      601 cgttcgcagc gacaccttgg aaaccgtacc aggcgtttcc atcatggacc tcaccgcgca
      661 acaggtaatc gcgccatcta cccccgaccg gtgccgctcc cgccatcgcg gtttcgtgaa
      721 cgttcccgta ttccgtcgcg gcaatccggt cccgcagatg ctccggcggg gtgaacatcg
      781 tgtctgtcat cccgaggggt gcaaagacct cccgcgtggc aaaactggcg acggattctc
      841 ccgtcacccg gcgcacgacc tcgccaagcg tgataaaccc ggcacagcta tagacaacct
      901 gctcgcccgg gggcatctcg ggctggtccc gcgcaaagct ctccagatac gcctccggac
      961 caacaccttc cataaaaacg ccccgccacg cgatcatgcc tgccgagtgc gacatcagcc
     1021 gccggatggt cacttctgct ttcttcccct cagtgccgaa ctcgggaaga atctggccaa
     1081 ccggctcatc aagatcgatc ttgccgtcgg ccacaagctt gagcacggcc ggcgtcgttg
     1141 ccaccacctt cgtcagggaa gccagatcca agatggtgtc catctccatc ggcgaccgct
     1201 cctcctccgg ctcacgcact gcccaccctg ttgcccgggc aaccacgatc tggccatgcc
     1261 gcacgatcag cgacaccgcg cccccgaatt cgtgcgcctc tgccgcctgc tccaggctgt
     1321 cccacgcctc gtcgagttgg gctgcgtcca tcccggcatc aaccggactc gttgttttga
     1381 aggtcattgt ggttcctctt gcctgatttc gggaagcgac gccacgtaag agccgacccg
     1441 gcgccgtttt cggacctccc ggagcatgga atacacccta cccctggcgc tcttcctcaa
     1501 ccgcaccggc cgcgatgagt gcatcgatct cttcatcgtt gtagcccatc ccggcaagca
     1561 cctcccgcgt gtgctcgccg aggaccggag gaccgtaccg cacctcgagt ggggtcccat
     1621 gaaagtgata gggaaagccc gtaacctgca cctcaccgag cctcttatgg ggagccgtca
     1681 tcaccagctc ctgatccacc gcctgcggat cttccaggat ctcgtcgatc ccgtagatcg
     1741 gcccacacgg cactgccgcg gcatccagcc gctccacgag atccgccgtt gtcatcgcac
     1801 tcaggcaacg gttcatgatc tcgtagagtt ccatccggtg cgcgacccga tcagcgttcg
     1861 tcaggaaccg ggagtcatcc cggacatcct ccaatcccat cgcatcgcag aaccgtcgcc
     1921 agatcgcatc gttcccgacc gcgatattca catagccgtc ggcactgggg aaggtatcgt
     1981 agggggtgat gatcgaatgg cggttgcccg acatcgtcgg gacttcgccg gtggcaaacc
     2041 agctggccgc ctgataggtc agcagcgcga tctgcccacc aagcatcgac gtatcgacat
     2101 actgaccgcg cttctccgga tgccgctccc gcgcgaagag cgccgcgacg acggcattgg
     2161 cgacgaacat accggcggtg atgtccgcga tcgggatgcc catcttcgtc ggcatcccgc
     2221 ccacctcgcc ggtgatgctc atcatgccgc tcatgccctg cacgatcagg tcgtaggcgg
     2281 tgcgattgcg ggagggcccc gtttgcccga agccggagat ggaggcgtag acgatgtgcg
     2341 ggttgcgctc cagcaccacc tcgtgcccga agcccagacg gtcgatcgtg cccggggaga
     2401 agttctccac cagcacatcc gcgtcgtcga tcagcttcca caggatctcc cggccaccct
     2461 ccgacttgag atcgagggcg atcgagcgct tgttgcggtt gacgctcatg aagtaggacg
     2521 cttcgccctg gacaaagggg ggaccccagt gccgggtatc gtctcccttg ccaggctgct
     2581 caatcttgat cacgtcggcg ccgagatcgc ccagcatcat cgtgccgtag ggacctgtca
     2641 tcactcgggt cagatcgacc accctgatcc cgctcagtgg tccggtcggc gtctcactac
     2701 tcacacagtc ccccagcaaa cgctatccac acgacgtggc atccgtcgtg tacttctgtc
     2761 tctatgggcg ccgattgtat caccggtgct atgggattcg ggcgggagtg tagcgagata
     2821 ctcctgtcat ggaccacgga tggagctgcg ctaccggctc atgccccaac gccggtcctc
     2881 gacaaaacca cgacgcgcct cacgctgctc ctccagccgc cggctgtcac ggaagaacca
     2941 gacaagcacc aatccggcca cgaacccgcc gatatgcgcg aaaaaagcga caccaccagt
     3001 ctgctcatca atcatcgaca gcgaccccac gcccgagatc acctggatca caatccagta
     3061 gccgatcatg acccatgctg gaatcatcgt cgtggtgacg aatatcccga ggaagatgag
     3121 cgtctggatc
//
LOCUS       DASYWM010000107         5645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_660306, whole genome shotgun sequence.
ACCESSION   DASYWM010000107 DASYWM010000000
VERSION     DASYWM010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660306"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..4357
                     /locus_tag="VGR29_04470"
     CDS             119..4357
                     /locus_tag="VGR29_04470"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEV2072879.1"
                     /translation="MIQLGPLRISYRLVLLAILLLAAALRFIGLDWDNGYYLHPDERF
                     MVMVTVDTAWPDSIFQYFDSATSPLNPYNTRHSTFVYGTFPLFLTKAVSAFFGTDVYG
                     EMHLAGRAISALADTGTVFLAAWIARRFFGRAAGLLAGFLLACTMLHIQTAHYYTVDA
                     VSVFFATASFAAIVKGWDRRSFGWFAVAGLMIGLAGASKPNYLIALAFLALPVAEMIR
                     HRGWEGLIPWGSTGFSRFPVLPAVALSGLVAFWTFRIAQPYAFTGPSIWNISLDQRWL
                     DDLSYWRHAQSGLVDVKSSIQWVDRPPVVYILDNLVRWGMGPPLGIAALSGLALLVVR
                     LIGARTWPSWWMLGMGAWSVVQLALYGTNMAQAQRYLLPIYPFLVVFSAGLLVELARR
                     FRPARWANPGAILIAVTVAYTLFYAVAFDTLFVRPLSREQASEWIYENVPAGSTLTAE
                     YWDDPLPMHLEGEDPSQYDVITLDLYAYEGPESLKLSTLVGQINQADYIVLSSNRIIG
                     SVPRQPERYPMATHYYEMLLSGDLGFELVADFSQKPELFGITLDDTNAEETLTVYEHP
                     YVRIFKKTDRFNVANVYNELDAALGYGGVNYIPGDPLGDQMFLTPEEQAAYAEEGSWS
                     EKFDRGTFTNAAPAFWWYVALQILALPALPLAWLVFRRFPDHGYAMAKVLGLLIVTWI
                     AWMLSSLKLLPFGPIAIALGWFLLLVVGLILLRRKSVQMLLAFRNRWRWLIATEVLFL
                     TTFAGMAWIRSLNPGFWQPTGEDATPFWYAVFNATVRSPYFPAYDPWLSGGQLHLSNW
                     GQMPWVTITRLTGIVPAIGYNLTFAGIFALLSVTLWTAASALIARVSSSRSHAGWIAL
                     LAPLLIVLGPTLMLARQVSEGAWGYAPRPDDWFIGGGAGDIAYGIWQIVTERLPSDPN
                     LARDAMRIGLDAEGNNPFGLMISGNLDQVTSTLPIIALAIAVAVTIVLRNHISGSEQW
                     RLDRTDLATVLLGGAAAGAMLASSTWGFLPTLGLIVAALGIRIVMAHCWERPWPMARN
                     AVLCAIAAAAFSYIAFFPAIQEFHATQRNLMTAPTVDLDESLSIYGPILFILVTWLLA
                     QVVASAQGVREPRWTARVISFLTLMIAAGALIASMVLSSLVLFVLVALLLVMIAVWHL
                     RARPAYLLVLVMTALAMMLMVLQNRIPIAADPFGGSSALQLGVFTWMLLGVAATVALG
                     GIVRWLEASRRLLRSIPGVIWTIALVALLAGSIFYPLLVTRSLVEARDDAPRTLDATS
                     LYSSDGQDLVLGDDRDAARWLMENVNGLPVILEATTSNYQLGGRISTMTGLPTIIGWS
                     TPEHIMRPGWTEVVDRRQAAVNEIFGSRGTFGSIDPLLRQYDVQLVYVGPVERATYSQ
                     ASLQKFETAVEEGDLEKLYQSGSVTVYYYPAWNQ"
     gene            5371..>5645
                     /locus_tag="VGR29_04475"
     CDS             5371..>5645
                     /locus_tag="VGR29_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072880.1"
                     /translation="MSSPCCSRLAIASNVAVTVAAPSGCTDDDEDMSMLSLANDAQRA
                     RRSGLLLKRSGTMVLTVNCLLKIHLGEGRAINRELELAIYDTDDSVG"
BASE COUNT          994 a         1821 c         1629 g         1201 t
ORIGIN      
        1 agccggagcg cttccgtcca gatccgggca gggttgcagc ccggtactca cttgcggcaa
       61 cggtggatgc ctatgagcgt ctctaccgtg gtgaagatgc tccgggggat cctctcccat
      121 gattcaactg ggtcctctcc ggatttccta ccggctggtg ctcctggcga tcctgctcct
      181 cgcggcagct ctccgcttca tcgggctcga ctgggacaac gggtattacc tgcacccgga
      241 cgagcgattc atggtgatgg tgacggtcga cacggcgtgg cccgactcca tcttccagta
      301 cttcgatagc gccacatcgc cgctcaatcc ctacaatacg cgtcattcga ccttcgtcta
      361 tggcaccttt ccgctcttcc tcacaaaggc tgtctcggca ttcttcggca ccgatgtcta
      421 cggggagatg catctggcag ggcgggccat ctcggcactt gccgatacgg gcacggtctt
      481 cctggccgcc tggatcgcac ggcgtttctt cggccgcgcc gcgggccttc tcgccggatt
      541 cctgctcgcc tgtacgatgt tgcatatcca gacggcgcac tactacaccg tcgacgcggt
      601 ctcggtattt ttcgccaccg cgtccttcgc ggcgatcgtc aagggctggg atcgacgcag
      661 cttcggctgg ttcgccgtcg ccggattaat gatcggactc gccggggcca gcaagcccaa
      721 ctacctgatc gcgctcgcct tcctcgcgct cccggtcgca gagatgatca ggcatcgtgg
      781 ctgggaaggg ctgatccctt ggggcagtac cggattctcc cgcttccctg tcctgccggc
      841 ggtggcgctc tccgggctcg tcgccttctg gaccttccgc atcgcccagc cctacgcttt
      901 cactggcccc tcgatctgga acatcagcct cgaccagcgc tggctcgatg atctcagcta
      961 ctggcgccat gcccagtcgg gattggtcga tgtcaagtcc agtatccagt gggtggaccg
     1021 gccaccggtt gtctacattc ttgacaacct cgttcgttgg ggcatgggtc cgccgctcgg
     1081 gatcgctgca ttgtcagggc tcgccttgct cgtggtccgg ctcatcggcg cacgtacctg
     1141 gccctcgtgg tggatgcttg gcatgggggc atggtcggtt gtacagctgg cgctctacgg
     1201 tacgaacatg gcacaggcgc agcgctacct gctgccgatc tacccctttc tggtggtctt
     1261 ttccgccggc ttgctcgtgg agctggcgcg ccgattccgg cccgcccgat gggcgaaccc
     1321 cggtgcgatc ctgatcgctg tcacggtcgc ctacacgctc ttctatgcag tggccttcga
     1381 taccctcttt gtccgcccgc tctcccggga acaggcctcg gaatggatct acgagaacgt
     1441 gccggccgga agcacgctga ccgcggagta ctgggacgat cccctgccca tgcatctgga
     1501 gggcgaagat ccctcgcagt acgacgtcat cacgctcgac ctctatgcct atgagggccc
     1561 ggagagcctc aagctcagca cattggtggg ccagatcaat caggccgact acatcgtcct
     1621 cagcagcaat cgcatcatcg gttcggtgcc ccgccagccg gagcgctacc cgatggccac
     1681 ccactactac gaaatgctgc tctccggaga cctgggcttc gagcttgtcg cagatttctc
     1741 gcagaaaccg gagctcttcg gtatcaccct cgacgacacg aacgccgaag agacactcac
     1801 cgtctacgag cacccctacg tccgcatctt caagaaaacc gaccggttca acgtcgcgaa
     1861 cgtctacaac gaactggacg ccgcccttgg atatggaggc gtgaattaca tacccggtga
     1921 tccgctgggt gaccagatgt ttctcacacc tgaagagcaa gcagcctatg cggaggaagg
     1981 ttcctggtcg gaaaagttcg accgcggaac gttcaccaat gctgctcccg ccttctggtg
     2041 gtatgttgcc ctgcagatcc tcgcgcttcc ggcgctgccg ctggcatggc tggtcttccg
     2101 ccgctttcca gaccatggat acgcaatggc gaaggtactc gggctgctga tcgtgacctg
     2161 gatcgcctgg atgctctcca gcctcaagct cctaccattc ggacccatcg cgatcgccct
     2221 gggctggttc ctgctgctgg tggtggggct catcctcctc cggcgcaagt cggtgcagat
     2281 gctcctcgcc ttccgcaacc gttggcggtg gctcatcgcc accgaggtgc ttttcctgac
     2341 caccttcgcc ggcatggcat ggatccgttc actgaatccc ggcttttggc aaccaaccgg
     2401 tgaggacgcg acgcccttct ggtacgccgt cttcaacgcg acggttcggt caccctactt
     2461 cccggcctac gatccctggt tgagcggagg gcagctgcat ctctccaact gggggcagat
     2521 gccgtgggta acgatcacgc ggcttaccgg gatagtccct gcaatcggct acaaccttac
     2581 cttcgccggc atcttcgcat tgctctccgt gacgctatgg acagcggcgt ccgcactgat
     2641 tgcccgagtt tcatcgtccc gatcccatgc cggctggata gccctgctcg ctcctctcct
     2701 gattgttctc gggccgaccc tgatgcttgc gcgccaggtc agtgaaggcg catggggata
     2761 cgcaccacgt ccggatgatt ggttcatcgg tggaggagcg ggggacatcg cctacggcat
     2821 ctggcaaatc gtcacggagc gactcccctc cgaccccaac cttgcacgtg acgcgatgcg
     2881 catcgggtta gacgccgagg ggaacaaccc attcgggctc atgatctccg ggaacctgga
     2941 ccaggtcacc agcacacttc ccatcatcgc ccttgcgatc gccgtggccg tcaccatcgt
     3001 cctgcgcaac cacataagtg gctccgagca gtggcgactg gaccggaccg acctcgccac
     3061 agtgctcctt ggcggagccg cagccggggc catgctggct tcctcaacat ggggattcct
     3121 ccctacgctg ggactgatcg ttgccgcgct cggcattcga atcgtcatgg cgcattgctg
     3181 ggaacgcccg tggcccatgg cacgaaacgc ggtcctttgc gcgatcgccg ccgcggcatt
     3241 tagctatatc gccttcttcc ccgcgattca ggagttccat gcgactcagc gcaatttgat
     3301 gaccgctcca accgtcgacc tcgatgaatc attatcaatc tatggaccga tactcttcat
     3361 tctggtcaca tggctcctgg cccaggtcgt ggcatccgcc cagggagtac gtgagccaag
     3421 gtggaccgcg cgagtcattt cgttcctaac cctgatgatc gctgctggag cgctaatcgc
     3481 atccatggtg ctctccagtc tcgtgctctt tgtgctggtc gccctgctcc tggtgatgat
     3541 cgcggtctgg catctgcggg cccggccagc ttacctgctt gtcctcgtca tgacggcact
     3601 ggcgatgatg ctgatggtct tgcagaacag gatcccgata gcggctgatc cattcggcgg
     3661 gagctccgct ctccaactcg gggtcttcac atggatgctg cttggagttg cggcgacggt
     3721 ggccctcggc gggattgtcc gatggcttga ggcatcgagg cggttgctgc gatcgatccc
     3781 cggggtgata tggacgatcg ccctcgtggc ccttctcgcc ggaagcatct tctacccgct
     3841 acttgtgacg cgatcacttg tggaggcgcg cgacgatgct ccccggacgc tcgatgcaac
     3901 cagcctgtac tccagcgacg ggcaggacct ggtccttggg gacgaccgtg atgcggcacg
     3961 atggctcatg gagaacgtca atggacttcc ggtgattctt gaggcaacga catccaacta
     4021 ccagcttggt ggacgaatca gcaccatgac cggcctcccg accatcatcg gctggagcac
     4081 tcccgagcac atcatgcgtc ctggctggac agaggtcgtc gatcgccgcc aggcggccgt
     4141 caacgagata ttcggcagcc gggggacatt tgggtcaatt gaccccctac tccggcagta
     4201 tgatgtccaa ctggtctatg tcggacccgt tgaacgagcc acatacagtc aggcatccct
     4261 ccagaaattc gagaccgccg tggaagaggg cgatctggag aagctctacc agtccggatc
     4321 ggtcacggtc tattactatc cggcctggaa ccagtaatgt tccacggcat gcatgaagag
     4381 gggcggtacc ggttttgctg gtaccgcccc tcttcagcct tcccttcccc tgtcacgtaa
     4441 gcgacaggcc ccttcgatag tggtcatcgt cggagtgctc gcctgaacgt atggtcttga
     4501 tgctctggct gccgcgtcaa gactatacgt acactgctta catgcacagt gtactcgata
     4561 tcctgtcacc tgaacagatt tgccaccttg tcactaacgt gatttctgcg ggcgtggagg
     4621 ggcccgatcc tgcatagtag acatttgaat ccgctcggga agcaggatac aggcaccgca
     4681 ggaccgatat gggagggttt gacgcatgcg ggagaaaggc tttccaggac cggtctccgt
     4741 attttgcgca acagcaccgt ccagggaatc cgtaaaatta cggatgccgg taccagcttc
     4801 tagatcctac attagagatg tcagggcgag aacctcatcc tgacacgaac ggcgaggtac
     4861 tggcatcccc cagctgccgc gtgcctcgcc ggcacgttcg ggcaccagaa catggctggg
     4921 gcatgacagc ggtttgcagg atcattgccg gtctcgcctt catggcgggg aagaacgaca
     4981 ttgctgcggc actgggacgc tgccgcgcac agacaatgcc gaaggcgttg agcgaatgcg
     5041 gcccatgcgg cccattaatt gctgtgcaat tgcctgtgtg tcgtccgcta cacggaacag
     5101 gcaacgcccc agcaaccatc atgcaactgg ctgtagaccg catagcacca tcgccagtct
     5161 ttcctacgct caaaggacgg caccagggga tgccctgcgt catgggcgtg cctggttgca
     5221 tgcctgttgg acgacgaatc ccagcaaccg gcgtggccac acatgggacg gaggcgcaga
     5281 tgcgcccagg tgtcgcccac cctgaggcaa tcctcacaac catccactgt ccctggagta
     5341 acgttctcat ttagtgcagg tgcgtacagg atgtcgtcac cctgttgctc ccggctggcc
     5401 atagcatcga acgtcgcggt caccgtggcc gcaccgtccg gctgcaccga tgacgatgag
     5461 gatatgtcca tgctctccct tgccaacgat gcccaacgtg cgcgccggtc tggcctactc
     5521 ctcaaacggt ccgggaccat ggtcctgacg gtgaactgtc tcctcaagat ccacctcggt
     5581 gagggccgtg cgattaacag agagctggag cttgccatct acgacaccga cgatagcgtg
     5641 ggtag
//
LOCUS       DASYWM010000108        11464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_623243, whole genome shotgun sequence.
ACCESSION   DASYWM010000108 DASYWM010000000
VERSION     DASYWM010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11464
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623243"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VGR29_04480"
     CDS             <1..762
                     /locus_tag="VGR29_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072881.1"
                     /translation="AEAEAQNVRGSEDSDWSSKWHSEERVDEAVGTGSSTDSTSPEDN
                     RWARNTTSDEAPVAEDESVTITASTAESDTTTTLGGGDAETGWATSLDDVEMDEVAQE
                     SETIIPAPDAVSTSSAGESSITGDESVSAADWDSPIAVDPSAPDTGEEQDPRERATTL
                     IEELRALVWKIGEEEPSGDNDIVTIANNLRSVRGETADFSDLREVIEAVGMNPRDIDS
                     LRDLGLQADRLQALLDSHASLTTALDEAIRSLQQN"
     gene            complement(891..2195)
                     /locus_tag="VGR29_04485"
     CDS             complement(891..2195)
                     /locus_tag="VGR29_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072882.1"
                     /translation="MTGIIHFPASSRRSRCVSRRTLIAGAACYAATTAFGPPALAQDD
                     AEPDAPVESFPERPLYEGNARRRLQSLLELVPVDALGGPDPNSWLFTWLDLQTHLTVM
                     GSPDLYADATNTAAILAPLVSDDPLFREALNEESRGIFGFSVFDVHQVLVAGVAPDQL
                     TYYAGGLPVADLPSTWEAAGYEKKSGEAGDYWTIGEDGELSLDLPAGRIGTGRLNNAA
                     ILNDGIVVFAGTAAQLQQVQALAGGGGESAAGLQDLDALISTMPLDAVNVIPVPGAGL
                     EAQSITPENPGAELNQTTTDLLAESDEAVGPMPEIDMALFGITAGIVAPLAARDDGTP
                     SATPEVLQSVADARFFVHLLAGSAADAVAAAEVVAWRIEHMISPVAGYPYSELLVPEF
                     STQDAAQGEVAALAFANPRALEAWHQMLAMQDIWPFVWLEAE"
     gene            complement(2411..3748)
                     /locus_tag="VGR29_04490"
     CDS             complement(2411..3748)
                     /locus_tag="VGR29_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072883.1"
                     /translation="MRLPSRHISLAGAVVVISVVAAIAVVVWVLLLVRDEESPVDPPP
                     EPTVSPLATPATPPPPGTVMATPVPNLSVEPAPEPGSLSDLLRYAPDRLADDTLPLSD
                     IARYADIKRWMDAGGVEIPDGPQDRAWEQWETGLGTLALPEVLAARANDPLWDATYGF
                     QLTDVDQVLAVGQAPDYVLIMRGDFEPEALHAAWVQSGYQAVRAEDVTIWSLFPGDAV
                     DLSAPASRPALGNLNNIVLLDDGTLIATSRLSRLEDVVRVAQEDAPSLDDNPAVAALL
                     APGAGAESMVTAVIVKGSLLATVASTPTVIATPFGATSTGPLPISTPSPSPIADLQMP
                     PVELMLAGIEAPGSGGTPPVFSMVLSFDAVEDATRAMISVDRTLQEDRSPVTHRSYRE
                     RLEPLQMRVIGTEQGDALLLIRTGLLNGSADWLVILEERDLGFLMWPLELDDE"
     gene            complement(3795..4787)
                     /locus_tag="VGR29_04495"
     CDS             complement(3795..4787)
                     /locus_tag="VGR29_04495"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEV2072884.1"
                     /translation="MAGPWDQISVFSGNSNPDLVDDICTYLSIPLGKAEVFKFSNDNT
                     FVRIQSSVRQDDVYVVQSFTTPVNDAIMELLIMIDTLKRASAGRITAVIPYYGYGRTD
                     KKDQPRVPITARLVATLIVQAGADRVLTVDLHAGQIQGFFDIPVDEMSAMYLTARYFV
                     EQHLENAVVVSPDLGNTKRARNFADILECSLAIIEKRRIGNQDKSEALNLIGSVEGRQ
                     VVIVDDEIDTAGSITQAADVCLEAGAKEVYATCIHPVFSGPAVERLKSSPIKEVVVTN
                     TIALPPEKRFEKLVVLSVAELLGEAIQRIHAGASVSTAYRRADAFQARLSFVTE"
     gene            complement(4876..5682)
                     /gene="rnc"
                     /locus_tag="VGR29_04500"
     CDS             complement(4876..5682)
                     /gene="rnc"
                     /locus_tag="VGR29_04500"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV2072885.1"
                     /translation="MTSPENRSVADRAISRPTPFDMPDSAAEQMLAAHIGITFHDPKL
                     FRLALTHRSVLHDWLLLEEVDAILQSNERLEFLGDAVLGAIVAERLYAVDPVADEGTL
                     TRHRVALVRAETLVKWARELHLHEGLYLGTGESVTNSVRDRMLAGAFEALVGAIHLDQ
                     GRDAAGCFVNAFLDRDMNAILADEASANPKGRLQEVVQERGLDQPMYSTVAEEGPDHA
                     REFTVEVSVDGEPMGTGRGRSKREAQQAAAREALLAIAARVQATEDRELR"
     gene            6438..7919
                     /locus_tag="VGR29_04505"
     CDS             6438..7919
                     /locus_tag="VGR29_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEV2072886.1"
                     /translation="MRVGNWRAEWFGNIRGDVLAGIVVALALIPEAIAFSIIAGVDPK
                     VGLYASFSIAVIIAFAGGRPGMISAATGAMALLMVSLVRDHGLEYLFAATILTGVLQV
                     IAGVLGLARVMRFVSSSVMTGFVNALAILIFMAQIPELQGVGWQTYPMVVAGLAIIYL
                     LPRVTTAVPSPLVSIVVLTIVTLILGLDVRTVDNMGELPSSLPVFLLPDVPINLETLM
                     IILPYALPLTVVGLLESLMTASIIDDFTDTGSDKNRESTGQGIANVATGFLGGMAGCA
                     MIGQSVINVKSGGRGRLSTFIAGAFLLVLVVGLGDAVGKVPMPALVAVMIMVSIGTFS
                     WRSLANLRTHPRTSSVVMLATVAVVVATHNLAYGVLVGVLLSGIFFAWKIGQIFGVHS
                     TISDDGRLRTYVVTGQVFFASAESFLSAFDFDEALDQVCIDVSRAHFWDISAVAALDR
                     VVLKFRRDATEVELIGMNEASATIVDRLGIHDKVDALDYVPGH"
     gene            7945..8799
                     /locus_tag="VGR29_04510"
     CDS             7945..8799
                     /locus_tag="VGR29_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2072887.1"
                     /translation="MKVLAFVDSSHYAVSVCDHAAWAASTLSVPVELLHVLERHRSDA
                     RIAADRSGRLGIDTRETLLAQIVALDEQRNRLAHESGRHLLDEAAARVRQGGVEGITQ
                     RLVHGTLVDNLREHERDAALVVLGKEGEGAGGPDSHLGSNLERVVRGSHRPVLVTSQS
                     FHPIRRFLLAFDGGASTGRAIDFLVNHPLLSDAEGHLLMVSEGTESHRYQLEDASTRL
                     QASGLSVSHHLRSGEPEHVIPQAVKEMEANLLVMGAFGHSRIRNLMVGSTTTALLATS
                     TVPLLIIR"
     gene            9123..9971
                     /locus_tag="VGR29_04515"
     CDS             9123..9971
                     /locus_tag="VGR29_04515"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEV2072888.1"
                     /translation="MKVFISVDIEGISGIVHADMMMPSETEYGRGRALMAGDANAAIE
                     GAVQAGADFILVADGHGPMRNLRIEDLHPAAHLVTGSANARDYCQLEGADSQPFDAAV
                     FLGYHAMAKTEKAIHPHTIAGVAVYELRVNGRPHGETGLNALMLASLGIPTVLVTGDE
                     TTCQEARSFLGESIETVAVKSATGRNAAICRPPSATAPEITAATKRALENSSNVTPYD
                     VGEALKIEIDFLTMPQCDRAARTAGIQRVGPMTITVEGSTPWEQYRNLWAGLRSALYE
                     PASWLA"
     gene            10407..>11464
                     /locus_tag="VGR29_04520"
     CDS             10407..>11464
                     /locus_tag="VGR29_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEV2072889.1"
                     /translation="MSAFLGLIKSRPVFYDGSMGATILNLGLTPEDYGGQDKEGYNDY
                     LAVVKPDVIETIHASFLEVGADVIETNTFGSSVIKAEEYGLGEQTYDINKTAAALARR
                     VADAYSTKDRPRFVAGSIGPSGLLPASEDPMLGNHRFREVVELFVPQIRGLMDGGVDG
                     LIIETVQDILELKATIFAARQVFEEMGKGVPIQASITLMPSERMLLGTDIAAALNILE
                     HLNVDIIGLNCSTGPDYMREPARYLGEYSTKPVSIIPNAGLPINVDGKAVFPMEPEPF
                     ARDLRNMVENFGVGIVGGCCGTTPDHIDQIIRDVTGLPVMARPMTPRPQVASMIRAVD
                     LAQEPSPTIIGERVNTLGS"
BASE COUNT         2262 a         3555 c         3515 g         2132 t
ORIGIN      
        1 gccgaagcag aggcgcagaa cgtgaggggc agcgaggact ccgactggtc gagcaagtgg
       61 cactcggaag agagagtcga tgaggcggtg ggcactggca gtagcaccga ctccacgtcc
      121 cccgaagaca atcgctgggc aaggaacaca acgagcgacg aagctcccgt ggctgaggac
      181 gaatcggtga cgatcaccgc atctaccgcg gaaagtgaca ccaccacgac actgggcggg
      241 ggagacgctg aaacaggatg ggcaacatca ctcgacgacg tggagatgga tgaggtagcc
      301 caggagtcgg aaaccatcat ccccgcaccg gatgccgtca gcacttccag tgcaggcgag
      361 agcagtatca ccggtgacga gagtgtctcc gcggcagact gggattcacc tattgcggtg
      421 gacccgtcgg ccccagacac aggtgaggaa caggatccgc gagagcgggc caccacgctc
      481 atcgaagaat tgcgcgcact cgtctggaag atcggcgagg aagagccgtc cggggataac
      541 gacatcgtca ccatcgccaa caacctgcgt agcgttcgtg gcgaaacggc cgacttctcg
      601 gacctgcggg aggtgatcga agccgtgggt atgaacccgc gcgacatcga ttccctgcga
      661 gatctcggtc tgcaggccga ccgtctccag gcgctcctgg acagccacgc cagcctcacg
      721 accgcgctgg acgaagcgat ccggtcactg cagcaaaact gatccgtgcc ggccagcaca
      781 acctacgtgc tgttcccgag atcacgaggc gatttccaag tgtccggctt atggcggtcc
      841 cgggttcttc aggcgcaggg ccgccatcgc ctaccttgcc ccaaacgctc ctactccgcc
      901 tccagccaca cgaacggcca gatatcctgc atggccagca tttgatgcca agcctccagc
      961 gctcgaggat tcgcgaaggc caacgcggca acctctccct gggccgcatc ctgggtcgaa
     1021 aactccggga ccagcaactc actgtaggga tacccggcga ctggcgaaat catgtgctcg
     1081 attcgccacg cgaccacttc ggcagcggcc acagcatcgg ctgccgaccc ggccaggaga
     1141 tgcacaaaga agcgcgcatc ggcgacgctc tggagcactt ctggcgttgc tgaaggagtg
     1201 ccgtcatccc gcgcagcaag tggagccacg atgccagcgg taattccaaa gagcgccatg
     1261 tcgatctccg gcatcggccc gacagcctca tcggattcgg caagcagatc ggtcgtcgtc
     1321 tggttcagct ccgcaccagg gttctccggt gtaatgctct gcgcttcgag cccggcgcct
     1381 ggcacgggaa tgacattcac ggcgtcgagt ggcatcgtac tgatgagggc gtcgaggtcc
     1441 tgaagaccag cggctgactc cccacctcca ccagcaagcg cctgcacctg ctgcagctgg
     1501 gccgcggttc cagcgaagac cacgatgccg tcattcagaa tggccgcatt gttcagcctg
     1561 ccggtgccaa ttcggcctgc aggcaaatcg agagacagct caccgtcctc accgatcgtc
     1621 cagtaatcac cagcttcccc gctcttcttc tcgtacccgg cggcctccca ggttgaaggc
     1681 aggtcggcca ccggcaggcc cccggcatag tacgtgagct gatcgggcgc gactcctgca
     1741 acaagcactt gatgaacatc gaagaccgag aacccgaata tgcctcgcga ctcctcattc
     1801 aaagcctccc ggaagagagg atcatcggac accaatggcg cgaggatggc ggcggtgttc
     1861 gtcgcatcgg catagagatc cggtgatccc attacagtca aatgtgtctg gaggtccagc
     1921 caggtgaaga gccaggagtt cgggtccggg ccaccgaggg catcgaccgg gacgagctct
     1981 agcaggctct gcaagcgcct ccgcgcattc ccctcgtaca agggtctctc cgggaaggat
     2041 tcgactggag catcgggctc cgcgtcgtcc tgcgcaaggg ctggcggccc gaaggcagtc
     2101 gtggcggcat aacaggccgc gccagcgatc agcgtacggc ggctcacgca gcgcgaccgt
     2161 cgggatgatg ccgggaaatg gatgatgccg gtcatggtgt ctcctctaac tcgtatctcg
     2221 cctgtgccaa tgcaagggat ttggtgaaag caacggtatg gatatctgca gaggctcttg
     2281 ttcaacgcgc gggtttcaga accggttccg cgcctccgtc cgccacataa gcgaatcgtg
     2341 cgacggggga ctgttacccc gaatccgaat gtatgaaccc atgccgattg atcatacaac
     2401 ccaagggaac ctactcgtcg tccaactcca ggggccacat caggaagccg agatctcgct
     2461 cttcaaggat caccagccaa tcggccgaac cattgagtag gcccgtgcgg atcaacagca
     2521 aggcgtcccc ctgctccgta ccaatgacac gcatctggag cggctccagc cgttcccggt
     2581 aggaacgatg tgtgaccgga gaacgatcct cctgcagggt tcggtccacg gagatcatcg
     2641 cccgcgtcgc atcctcaaca gcgtcgaagc tcaggaccat cgagaacact ggaggagtcc
     2701 ctcccgagcc aggcgcctcg atcccggcca gcatcagctc gaccggaggc atctgcagat
     2761 ccgctatcgg ggacggcgat ggcgtactaa tcggcaaggg gccggtggag gtcgcgccga
     2821 aaggggtcgc aatgaccgtc ggtgtcgaag caacagtcgc aagcagggat cccttgacga
     2881 tcacggccgt caccatcgat tccgcccccg cgcccggcgc gaggagtgcc gccactgcgg
     2941 gattgtcgtc cagggaaggc gcgtcctcct gcgccacgcg aacaacatcc tccagccgtg
     3001 agagccggga tgtcgctatc agggttccat cgtccagcag cacgatattg ttgaggttac
     3061 ccagcgcggg gcgactggcg ggtgccgaga gatcaaccgc atctccggga aagagcgacc
     3121 atatcgtcac gtcctccgca cgcacggcct gatagccgga ttgcacccag gcagcgtgca
     3181 gggcctccgg ctcgaaatct ccccgcatga tcagcacgta gtcaggcgcc tgaccgaccg
     3241 caagtacctg atccacgtcc gttagctgaa acccgtatgt cgcgtcccag agagggtcgt
     3301 tggcacgcgc cgcgagcacc tcgggcaatg cgagcgtccc aagccccgtt tcccattgct
     3361 cccaggcccg atcctggggc ccatcgggta tttccactcc ccctgcgtcc atccagcgct
     3421 ttatatccgc ataccgggcg atatcgctga ggggaagggt gtcatcggcc aatcgatccg
     3481 gcgcgtagcg gagcaagtcg gagaggcttc cgggctctgg agccggctca acggaaaggt
     3541 ttggcacagg tgttgccatc accgtgccag ggggcggcgg agtggccggt gtggcaagcg
     3601 gggagactgt gggttccggc ggcgggtcaa ccggtgattc ttcgtcccgg acgagcagga
     3661 gcacccagac caccaccgcg atcgcggcaa ctacagagat gacgaccacg gccccggcga
     3721 gcgagatatg gcgactgggc agtctcacgg ccagttcgct gaacgccggt aacccagcgc
     3781 aggggcagtc atcgctactc ggtcacaaac gatagacgtg cctgaaaggc gtcagcacgc
     3841 cggtaggcgg tcgagacgct ggcgccagca tggatgcgct ggatcgcctc tccgagcaac
     3901 tcggcgaccg agaggaccac cagtttttca aatcgcttct cgggcggcaa ggcaattgtg
     3961 ttggtcacca cgacctcctt gattgggctg gacttgagcc gctcgactgc tggaccggag
     4021 aagacgggat ggatgcatgt ggcatagacc tctttcgcac cggcctcgag gcacacatcc
     4081 gccgcctggg tgatcgagcc agcggtgtcg atctcgtcgt cgacaatcac cacctgccga
     4141 ccctcaaccg aacctatgag attgagcgcc tccgacttgt cctgattgcc gatccggcgc
     4201 ttctcaatga tcgccagtga acattcgagg atgtccgcga agttgcgggc acgcttggta
     4261 ttgcccagat caggagagac gacgaccgcg ttctccagat gctgttccac gaaatagcgc
     4321 gcggtgagat acatcgcgct catctcatcc accgggatat cgaagaagcc ctgaatttga
     4381 ccggcgtgga gatccactgt caacacccgg tcggcaccgg cctggacgat cagcgtggcc
     4441 acgagacgcg ccgtaatcgg cacacggggc tggtctttct tgtcggtccg gccatagcca
     4501 taataaggga tcacggcggt gatccggccc gctgaagctc gcttcagcgt gtcgatcatg
     4561 atcagcaact ccatgatcgc gtcgttaacc ggtgtggtga acgactggac aacgtagacg
     4621 tcgtcctgcc ggacactgga ctggatgcgc acgaaggtgt tgtcgttgct gaatttgaag
     4681 acctcggcct tgcccagggg aatgctgaga taggtgcaga tatcgtcgac caggtccgga
     4741 ttcgagtttc cgctgaatac cgagatctga tcccaaggtc ccgccatcgt tccctcatcc
     4801 ttcccatgtc gggcacggga gcacacaatg gtacgctccc gctcattcct tcggaattgc
     4861 ctattcggcg ctcccctacc ggagctctct gtcctcggtc gcctggacgc gcgccgcgat
     4921 agcaagcagc gcctcacgcg cagctgcctg ctgcgcttcc cgcttactac gcccgcgccc
     4981 ggtacccatg ggctccccgt ccacagagac ctcgacagtg aattcacgcg cgtgatcggg
     5041 gccctcttcg gcaacggtgc tatacatcgg ctgatcgagc ccgcgctcct gcacgacttc
     5101 ctggaggcgc ccctttgggt tggcgctggc ttcatcggcc agaatagcgt tcatgtcgcg
     5161 atcgagaaag gcgttgacaa aacaccctgc ggcatcacgt ccctgatcaa ggtggatcgc
     5221 cccgacaagt gcctcgaagg caccggccag catccgatcc cggacgctgt tggtgacgct
     5281 ttcgccagtg ccgagataga gcccttcatg cagatggagc tcccgcgccc acttcaccag
     5341 ggtttctgcc cgcaccagtg ccacccggtg acgggtcagc gtcccctcgt cggcaaccgg
     5401 gtcaacagcg tagagccgct ccgcgacgat ggccccaagg acagcatcgc caaggaactc
     5461 caaccgctcg ttgctctgca gaatcgcatc gacctcttcg agcaagagcc agtcgtgcag
     5521 gaccgagcga tgcgtcaggg ccagccgaaa gagcttcggg tcgtggaagg tgatgccgat
     5581 atgtgccgcc agcatctgct cggccgccga atcgggcatg tcaaatggtg ttggacggct
     5641 gatggcccta tctgcgacgg agcggttttc aggggacgtc atgctaatgg ggtacgcctt
     5701 ttggaatgcg tgaggagtgg caaacgcgaa gggatcacac gggccatacg ctgacacgag
     5761 tatacgcggc atgccgacgt cgcttggtga agttgatcca gagcggcgtt cacaagatct
     5821 gcgtagaagc tgatggagtt tgagaggagt tcttaggtgg ccaagcgttc taacctggcc
     5881 agtggcagcc taagggggtg cggagcccac tttcgccgag atgacgggga tttctccaga
     5941 ctgccaagtt tttggatgga ggtcaaaaac ttggccatct gggcggcttt atcgaatatc
     6001 gaagtctccg gcgaggatcg aatcaagcag atcgactcgg ctcgtctccc tcggaccagg
     6061 ccatgacttc gcaagtactc ccctcaatgc ccgagcagac acggattatc cgaagttggc
     6121 gccaggctgc ctagatggcc aggttgttgt tttagggggt gttggcagtg tgggtgcacg
     6181 gggcgggggc gggttcccta cctcccgtca gcgaaccgcc gctcatgcag ccaatctttg
     6241 tccacctctc ctgacgtgac gacatcgcag atgtccctcc tccgttgccc ggtccggaat
     6301 actctggtag gcttccccgg ttgcccgcta ccggggcggc attccattga atgaacaatc
     6361 ggataacgat gatgagacgc ccaagggccc ggttcgcgtg aaccgaggcc acgggcgatg
     6421 ttgggatgga ctgaaacatg agggttggaa actggcgtgc cgaatggttc ggcaatatac
     6481 gtggggatgt gctggccggg atagtggtgg cgctggcact catcccggag gcgatcgcct
     6541 tctcgatcat cgcgggcgtt gaccccaaag tcggcctcta cgcatctttc tcgatcgccg
     6601 tcatcatcgc cttcgccggt ggccgacccg gcatgatcag tgcggccacg ggcgcgatgg
     6661 ccctcctgat ggtaagcctc gtcagggatc acgggctgga gtacctcttc gccgcgacca
     6721 tcctgaccgg tgtattgcaa gtcattgccg gagtcctcgg cctcgcgcgg gtcatgcgct
     6781 ttgtctccag ctcggtgatg accggcttcg tcaatgccct ggccatcctg atcttcatgg
     6841 cgcagatccc cgaattgcag ggcgtcggat ggcagaccta ccccatggtt gtcgccgggc
     6901 tggcgatcat ctatctcctg ccacgtgtaa cgaccgctgt gccctctccc ctcgtcagca
     6961 tcgtcgtcct gaccatcgtc accctcattc tcggtctgga cgtgcggacc gtggacaaca
     7021 tgggcgagct gccatcgtcg ctgcctgtct tcctgcttcc ggatgtcccg atcaacctcg
     7081 aaacgctgat gatcatcctg ccctacgcac tgccactcac cgtcgttgga ttgctggaat
     7141 cgctcatgac cgcctcgatc atcgatgatt tcaccgacac gggcagcgac aagaaccgtg
     7201 aaagcaccgg ccaaggcatt gccaatgtcg ccaccggctt cctcggtggc atggcagggt
     7261 gtgcgatgat cggtcaatcc gtgatcaatg tgaagtccgg tggccgtggt cgcctttcca
     7321 cgttcatcgc gggcgccttc ctgcttgtgc tcgtcgtcgg cctcggcgat gcggtcggaa
     7381 aggttcccat gccggccctt gtcgccgtga tgatcatggt gtcgatcggt accttcagct
     7441 ggcgatcact ggccaacctg agaacgcacc ctcgcacctc aagtgtcgtg atgctcgcca
     7501 ccgtagccgt ggtggtcgcc acgcacaacc ttgcgtatgg tgtattggtc ggcgtgcttc
     7561 tcagcggcat cttcttcgcc tggaagatcg ggcagatctt cggcgtgcac tccacgatct
     7621 ccgacgatgg gcgattgcgc acctacgtgg tgacgggcca ggtcttcttt gcctcagccg
     7681 aatcgttcct cagtgccttt gatttcgatg aggcgctcga ccaggtctgc atcgacgtaa
     7741 gccgggccca cttctgggac atctccgccg tcgccgcgct ggacagggtg gtcctgaagt
     7801 tccggcgcga cgctaccgaa gtcgaactca tcggcatgaa cgaggccagt gcgacgatcg
     7861 tcgacaggct tggtattcat gacaaggtcg atgccctgga ctatgtcccc ggccactaga
     7921 gcacgcagca ggaggagagt agtaatgaag gtcctggcgt ttgtcgatag ctcccattac
     7981 gcggtcagcg tgtgcgacca tgcggcctgg gctgccagca cactctccgt tcccgttgag
     8041 ctccttcatg tcctggagcg tcaccgctcc gatgcgcgaa tcgccgccga ccggagcggc
     8101 cgccttggca tcgacacccg ggaaacgctg ctcgcccaga tcgttgcgct ggatgaacag
     8161 cgaaaccggc tggcccacga aagcgggcga catctgcttg atgaagccgc cgcacgagtc
     8221 cgccagggag gggtcgaggg aatcacccaa cggctggtgc atggcacgct ggtcgacaac
     8281 ctgcgcgagc acgaacgcga cgccgcattg gtcgtgcttg ggaaggaggg tgaaggcgcg
     8341 ggaggccctg acagtcactt gggttccaac ctcgagcgtg tcgtccgtgg aagtcaccgg
     8401 ccggtcctgg tgacatccca atcattccat cccatccggc gctttctcct ggccttcgac
     8461 ggcggagcaa gcaccggacg cgcgatcgat tttcttgtga accatcccct acttagcgat
     8521 gccgaaggac acctgctgat ggtcagtgag ggaaccgagt cccaccgcta tcaactggag
     8581 gatgcgagca cgcgactgca ggcatcgggg ctctcggtct cccatcatct ccgttccggg
     8641 gaacccgaac atgtcatccc gcaggccgtg aaagagatgg aagccaacct gctcgtgatg
     8701 ggtgctttcg ggcactcccg catccgcaac ctgatggtgg gcagcacgac aacggcctta
     8761 ctggcaacaa gcacggtgcc tttgctcatc atccggtaga gctgtcttta gagaggttgc
     8821 atgtctgtag cgaacaggca atagccagcc ctgagcaggc cgcaaaggac gacattgctg
     8881 cggcaagtcg tccgctacac cttccgcctg aagtccaggc cttgcatcag gaaccgtcgt
     8941 tgccgccgac ggggtagaac ctgcggtcat gaatgtgact gtctgggagt tgcactgcct
     9001 ccctgttttc gagaaatgag tatcggcttg ggattccgga gagactgtgt tcgccgcccg
     9061 ctttcatgat gtgcgatggt agggcgaaga gctgttcagc catacaggtg aaaggatttc
     9121 ccgtgaaggt cttcatctcc gtcgatatcg agggtatctc aggcatcgtt cacgctgaca
     9181 tgatgatgcc gtcagagacg gaatacgggc gtggacgcgc gctcatggct ggcgatgcca
     9241 acgcagctat cgagggcgcg gtgcaggcag gagcagattt cattctcgtc gcggacggcc
     9301 acggccccat gcggaacctg cgcatcgagg atctgcaccc ggcggcgcat ctcgtcacag
     9361 gcagcgcgaa cgccagggac tattgccagc tcgaaggggc cgacagtcaa ccgttcgatg
     9421 ccgcggtttt cctcgggtat cacgcgatgg cgaagacgga gaaagcgatt catcctcata
     9481 cgattgcagg tgtggcggtc tatgagctcc gggtaaatgg gcgaccacat ggagaaaccg
     9541 ggctcaacgc cctgatgctg gcgtcgcttg gtatcccgac ggtgcttgtg accggagacg
     9601 aaacgacctg tcaggaggcg aggagttttc tgggggaaag catcgaaacc gtcgcggtga
     9661 aatcggcaac cggcaggaat gcggcgatct gccgtccacc gagcgcgaca gcgccggaga
     9721 ttaccgccgc gacgaaacgc gcgctcgaaa actcgtcgaa cgttacccct tatgacgtgg
     9781 gcgaagcgtt gaagatcgag atcgatttcc tgacgatgcc gcaatgcgat cgggcagccc
     9841 gtacggcggg gatccagcga gtgggcccga tgacgatcac ggtcgaggga agcacgccct
     9901 gggagcagta tcggaacctg tgggcaggtc tccgctctgc cctctatgaa ccggcaagct
     9961 ggctggcgta agcccagttc tgtctgagat tcaagctccc ttagacacgg tacgcgagct
    10021 gcggtgcatg atgggcccgg cgtgcgttgc cgggcccatg cgcatccccg gcagatgcag
    10081 tccaaggtgt gcctcaccgc gcaaaaccgg cttcgaggcg agagtcgtcc tggccggacg
    10141 cgagggacga cattgctgcg gcaagtcgtc cgctacaccc tccacttggc gaacacgggt
    10201 atcaatctca ttaatcgctg taatgggctg gtgctctacg tgaagaagag catgggcatt
    10261 gccgccttcc agcctgccgc gtcgcgtcac gatgggacaa aatcgtgttc aatagactgt
    10321 gacggttcat cgcgtcgtta acctgattcc ggcaccgcga ttccggatat ccatcgtacc
    10381 tggaaacccc acggagggag accgtagtga gtgcatttct cggcctgatc aagtcacgcc
    10441 ccgtcttcta cgatggctca atgggcgcga ccatcctcaa tctcggactg acgccggaag
    10501 attacggtgg gcaggacaag gaagggtata acgactacct cgcggtcgtg aagcccgatg
    10561 taatcgagac gatccacgcc tccttccttg aggttggcgc ggatgtcatc gagaccaata
    10621 ccttcggcag cagtgtcatc aaggccgagg aatatggcct cggtgagcag acctacgata
    10681 tcaacaagac ggcagccgcg cttgcccgga gagtcgccga cgcgtacagc acgaaggatc
    10741 gacccaggtt cgtggcaggc tccatcggcc cctccgggtt gctccctgct tcggaagacc
    10801 cgatgctcgg gaaccaccgg ttccgcgagg tcgtcgagct cttcgtgccc cagattcgtg
    10861 gcctcatgga cggtggcgtc gacgggctga tcatcgagac cgtgcaggac atactcgagc
    10921 tcaaggcaac gatctttgct gcccgccagg tcttcgagga gatgggcaag ggggtgccga
    10981 tccaggcatc catcacgctg atgccatcgg agcgcatgct gctcggcacg gacatcgcgg
    11041 cggcgctcaa catccttgag cacctgaacg tcgacatcat cggcctcaat tgttcgaccg
    11101 gacccgacta catgcgtgag ccggcgcgct atcttggtga atacagcacg aagccggtct
    11161 caatcatccc gaacgcgggc ctgccgatca atgtggacgg caaggcggtc ttcccgatgg
    11221 agcccgagcc gtttgcccgc gatctccgca atatggtgga gaacttcggg gtcggcatcg
    11281 tcggcggctg ctgcggcacg acgccggacc acatcgacca gatcatcagg gacgtcaccg
    11341 ggctgccggt catggcgcgt cccatgacgc cgcgaccgca ggtagccagc atgatcaggg
    11401 cggtggatct ggcacaggaa ccgtcgccga cgatcatcgg cgagcgggtc aacaccctcg
    11461 gctc
//
LOCUS       DASYWM010000109         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_59891, whole genome shotgun sequence.
ACCESSION   DASYWM010000109 DASYWM010000000
VERSION     DASYWM010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59891"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1996
                     /locus_tag="VGR29_04525"
     CDS             <1..1996
                     /locus_tag="VGR29_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072890.1"
                     /translation="LWPINDMFATYTPQSHNAMADRTENDFNALTDLLLRLHRDYDYL
                     DEDMLASADVSGAEIRIGDEAYQVVILPPIRHLKLSTIERLEQFVAGGGCVLGTVFLP
                     DTGLGEGQRVDICDRVRQLFDVDPAETQRSFTSQPSITRTVTDHPGGGKTAFLQSYAL
                     NRQLPRRLQESMGVPGQPESSHIVIEEVSGQTTYWFDPSEGERRDITGELTAEREEVM
                     RALGEAIDTLIAPDITIDNPEVFYLHRVKDDRDLYFFVNPTYQPQTAQVVLSKELRPV
                     LWDPSDGEERPIAPWTIETGSTSFEIQLPPTGSAFVLAGDPTPVRIGETDTVVTSITQ
                     DHVRGYITGEQGSIVLDNGRQHRQRVGSGVVPEAVSLDGEWAFQAEDDNALIISRWLA
                     SERPLDSKTALDTASTDGWLPMVPGAWAYQLPSEPADPYPVDVWYRLSFEATSLPPAL
                     ELIIDGFASSDWEVYLNGRPVDGVAQRSKIDSQMQRLDLTPLAQIGENVITVRLTVTK
                     ATDGLLDLVKITGDFAIEETGEDVYRIVPPRATMQAASWTEQGYPFYSGRGVYQRTFT
                     IPTEAEGKRIILSPAMTDDVLEVVINGTRAGVRLWADYDVDITDLVKPGENVLELRVA
                     NTLINLLEAVPRASGLAGAPTLGMYEQFDLELTSVLATAD"
     gene            2009..2530
                     /locus_tag="VGR29_04530"
     CDS             2009..2530
                     /locus_tag="VGR29_04530"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEV2072891.1"
                     /translation="MRIPVEELTPDAFAPYGAVIEQPDATADASGSGWSWWSEISRLP
                     QADRPYAVGYLNLQPSAMEFDWAERHLKSAEVIIPLGGACLVYVGPPDQTPVWDQFRV
                     YRVRPGQAVVLKEGIWHGAPLALDHPLTALVLLRQGTGTEDVYKSERTEGPMHVVPNE
                     TRMGEGERTHASR"
BASE COUNT          535 a          817 c          835 g          469 t
ORIGIN      
        1 gctctggccg atcaacgaca tgttcgccac ctacacgccg cagagtcaca atgccatggc
       61 ggaccgtacg gaaaacgact tcaacgcatt gaccgacctg ctgctgcgcc tgcaccgcga
      121 ttacgactac ctcgacgagg atatgttggc ctcggccgac gtttccggag cggaaatcag
      181 gatcggtgac gaggcatacc aggtcgtcat cctgccgccg attcgacatc tcaagctgag
      241 tacgattgag cggctggagc agttcgttgc cgggggcggc tgcgtcctcg ggacggtctt
      301 cctgcccgac acgggactcg gcgaggggca gcgggtggac atatgcgacc gggtccggca
      361 gctcttcgac gtcgacccgg cagagaccca gcgaagcttc acctcgcagc cgtccattac
      421 caggaccgtc accgaccatc cgggtggagg gaaaaccgca ttcctccaga gctacgcgct
      481 caatcgacag cttccccggc ggctgcagga gagcatgggc gtgccagggc agccggaaag
      541 ctcacacatc gttatcgagg aggtttctgg ccagaccacc tactggttcg acccgtccga
      601 gggtgagcgg cgggacatca ccggcgagct taccgccgag cgcgaggagg tgatgcgggc
      661 gctcggggag gcgatcgaca cgctcatcgc acccgatatc acgatcgaca atccggaagt
      721 cttctacctg cacagggtga aggacgatcg tgatctctac ttcttcgtga accccacgta
      781 ccagccgcaa actgcccagg tggtcttgag taaggaactc cgcccggtgc tgtgggaccc
      841 ctcagatggt gaagagcggc cgatcgctcc ctggaccatc gagacggggt ccaccagctt
      901 tgagatccag ctgccgccaa ctggatccgc gttcgtcctt gcgggtgatc cgacaccggt
      961 tcgcattggg gagacggata cggtcgtgac gtcgatcacg caggaccacg ttcggggtta
     1021 tatcaccggc gagcaaggat cgatcgtgct cgacaatggc aggcagcacc ggcaacgcgt
     1081 cggatccgga gttgttccgg aggcggtctc cctcgacggt gagtgggcgt tccaggcgga
     1141 ggatgacaat gcgctcatca ttagccgatg gctcgcgtcc gagcggcccc tggatagcaa
     1201 gacggccctc gacaccgcca gcaccgatgg atggttgcca atggtgcccg gcgcctgggc
     1261 atatcagctc ccctcggaac cagccgaccc ctacccggtc gatgtttggt accggctgtc
     1321 gtttgaagcc acctccctgc caccggcgct ggagctcatc atcgatggct tcgccagcag
     1381 cgactgggag gtctacctca atggacgccc ggtcgatggc gtggcccaac gaagcaagat
     1441 cgacagccag atgcaacggc tggacctcac gccactggcc caaataggcg aaaacgtcat
     1501 caccgttcga ttgacggtta ccaaggccac cgacgggcta ctcgatctcg tgaagatcac
     1561 cggcgacttt gcgatcgagg agaccgggga ggatgtctat cgcatcgtgc cgcctcgcgc
     1621 caccatgcag gccgcctcgt ggactgagca gggctaccca ttttactcag gccgtggcgt
     1681 ctaccagcgc acattcacca tcccgaccga agcggaaggg aagcggatca tcctgagccc
     1741 ggccatgacc gacgacgtgc tggaagtggt gatcaacggc acccgggcgg gggtccgttt
     1801 atgggccgac tacgacgtag atatcaccga cctggtgaaa ccaggtgaga acgtccttga
     1861 gcttcgtgtt gccaacaccc tgatcaacct gctggaagcg gtgccgagag cgtccggcct
     1921 ggctggagca ccgacgctcg ggatgtatga gcagttcgat ctcgaactca cttccgtcct
     1981 ggcgaccgcg gattaggagg gtacggagat gcgtatccct gtcgaggagc tgacaccgga
     2041 cgcctttgcc ccatatggcg cggtcatcga gcagcccgac gcgacggccg atgcgtccgg
     2101 cagcggatgg agctggtgga gcgagatttc gcgacttccg caggccgatc ggccctacgc
     2161 ggtcggttac ctgaatctcc agccgtccgc aatggaattt gactgggcag agcgccacct
     2221 gaaaagtgcc gaggtgatca tcccgcttgg tggagcgtgc ctggtctacg ttggccctcc
     2281 tgaccaaacg ccggtctggg atcaattccg agtctaccga gtgcgtccag gacaggcggt
     2341 cgtgctgaag gaggggattt ggcacggcgc gccacttgca cttgaccatc cgcttaccgc
     2401 gctggtgttg ctgcgccagg ggaccggtac agaggacgtc tataaatcag aacgtaccga
     2461 gggtcctatg cacgttgtgc ccaacgaaac aaggatgggc gaaggagaaa gaacgcatgc
     2521 cagtcgttga ccgacgcgaa ttcggcggac gcttcggggt caaagaaaac tcccagcgcc
     2581 ttgccaacta ccggtatctc gaagtgcagt tgatggagat gatcggcgga tggtcgcata
     2641 ccacgccgca gctcgc
//
LOCUS       DASYWM010000110         7387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_660462, whole genome shotgun sequence.
ACCESSION   DASYWM010000110 DASYWM010000000
VERSION     DASYWM010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660462"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VGR29_04535"
     CDS             complement(<1..858)
                     /locus_tag="VGR29_04535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV2072892.1"
                     /translation="MNPDQVRAVLARWFPDGELVISRAEGGVSTPVFRALVGEEQFWV
                     RLGEEPRERRDGEVAAHRLLRAAGVPVPNVIRYEVAPPELDRSITLTSHLTGRSLAGI
                     QVAPWLPALAADVGRLLARINNIPVRGFGWASASEEHGVPAGEHRSRSGWTREYVDAV
                     RMVRESGVLDASALDHLQAAIERWTRILDRTTAALAHGDFDTTHIYVDPEDGTLTGVI
                     DLGELRGADPLYDLGHLLLHDGEVGRPKLFPHILDGYAEIAPLPDDAMAQIRLQALVI
                     GTRALSIQLQ"
     gene            complement(953..1993)
                     /locus_tag="VGR29_04540"
     CDS             complement(953..1993)
                     /locus_tag="VGR29_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV2072893.1"
                     /translation="MDISRIYFSADEDYLSAAADELRPHFPDSFMEPVGPDLGAFEDE
                     GVSISLIARTIQEQHLVFPRHLMRGVGMAELEEAQDLAELGDIALGLWEQFPLPGEVS
                     LHVWQSGQVDLAYRTDQLWRRLASALTDMDIEVKRGGAAHILSACVTEQGIVFGSNGA
                     ANALTDWPGGRVRLAKPKGQISRAEFKLEELFRTDAVTLPQGGTALDLGASPGGWSRI
                     LLERGFEVWAVDPGELDARLSNRQGLHHVKTTAGPFLAENRERFDLVVNDMRMAPVLS
                     ASVMNSAAGHLAPDGLGIMTVKLQPTDPLRVVDETFRMLSRAWEIVFARQLHHNRNEI
                     TVVIRAKTVASR"
     gene            complement(2661..2912)
                     /locus_tag="VGR29_04545"
     CDS             complement(2661..2912)
                     /locus_tag="VGR29_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072894.1"
                     /translation="MTHFFKLQVDLDDLTDAMYGFVTDPTPRRNELQVVKELDEINPE
                     IVDVLECMVLDGTEERQDVADYVNAVFGSHGLGPIKAES"
     gene            3173..4684
                     /locus_tag="VGR29_04550"
     CDS             3173..4684
                     /locus_tag="VGR29_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEV2072895.1"
                     /translation="MDNTKPVLTNRQGHPIHNNQQMSTVGTRGPATLENYHFLEKISH
                     FDRERIPERVVHARGTVAHGEFEAYGTIGDEPASTYTRAKLFQEKGKKTPVSVRFSTV
                     AGGRDSSEVARDPRGFAVKLRTEDGNWDLVGNNLPVFFIRDATKFPDFIHSQKPDPVS
                     NQANDPQRAFDFWAASPEAMHMLMWLFSPRGIPKSYRHQDGFGVNTYRMVNAKGEGVL
                     VKYHWKSQQGIESLTAAQAAEIQAKELGSHTKDLFEAIERGDYPKWELNVQIMSDDEH
                     PELDFDPLDDTKTWPEDQFPLRPVGMMTLNRNVKDNFAENEQIAFGTGVLVDGLDFSD
                     DKMLVGRTFSYSDTQRYRVGANYLQLPVNQPVTDVHTNQEGGMMSFGHDERGADPSVN
                     YEPSVLAGMVEADESYREYTPYVEGHIVRESIDRTNNYGQAGERYRTIEQWERDDLVL
                     NISTGLSQCAPILQEKMIEHFTRCDKELGQRVAEGIRSMSESQSEKAPMTTLA"
     gene            complement(4760..5692)
                     /locus_tag="VGR29_04555"
     CDS             complement(4760..5692)
                     /locus_tag="VGR29_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEV2072896.1"
                     /translation="MVDTRVPVILDTDIGTDVDDTLALAVLLGSPEIDLLGVTTVYGD
                     VALRARMARKLLALRGRDNVTVYEGIAEPLMRNRPVYWPGHEGVGLLEAGDDARFGQS
                     PDQHAVDYLIESVMARPGKVHLLAVGPLTNVAAALIREPKLASNLAHLTIMGGVIATW
                     TGVRGAAEHNIQCDPEAARVVFASGAPISLVPLDVTLKVDITQEGVEAIQAVGTPFHE
                     AVADQVARYPGFQEREGRTSLHDPLAAAAVLQPDLLQWHDLVVDIELAGRLTTGMTVA
                     RQPGLETPSTARVAMDVDVDAAEQFITDRICGNS"
     gene            complement(5912..6430)
                     /locus_tag="VGR29_04560"
     CDS             complement(5912..6430)
                     /locus_tag="VGR29_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072897.1"
                     /translation="MRRTSGFLFTIAVMVLGLIALVGLISGAGAQDASPPAGGPPPGA
                     FEIAPGVTADAVVFAEGRQDPSLYRLTFAPGVTYMVAESPALELAYVESGALSMTLSV
                     PVTIGQVGTPDAAGESVEANTEFMLEAGQYIVLTPGTTGEVRNDGDEPGVVSIANIIP
                     AAVGTPEATPAS"
     gene            complement(6662..7336)
                     /locus_tag="VGR29_04565"
     CDS             complement(6662..7336)
                     /locus_tag="VGR29_04565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2072898.1"
                     /translation="MNRLRRIGLDRSPNDLQRISRIYNERAPTWDEREGRGENLVLGP
                     AYRDRLARMLEGDVLEIGVGTGETLRRVQALGAPIASFTGIDVSTGMLAEASKYAESS
                     PFPVTLRQMNAESLAAFSADTFDTVTASLVLCTVPDPVSALHEMARVVKPGGKIVLIE
                     HVLAKNPIVGTAQKVLAPLQRRHMACNIDRPTDRMLRDLGFQVERDDSRLLGVVHLMV
                     ARPPGG"
BASE COUNT         1524 a         2400 c         2144 g         1319 t
ORIGIN      
        1 ctgaagttgg atcgacagtg cgcgggtgcc gatgaccaga gcctggagac gaatttgagc
       61 catcgcgtca tccgggagcg gggcgatctc cgcatatccg tcgaggatgt gtggaaagag
      121 cttcggtcga cctacttcac cgtcatgcag caggaggtga cccagatcgt acagcggatc
      181 cgcaccacga agctcgccca gatcgatgac accggtcagg gtgccgtctt ccgggtcaac
      241 gtagatgtgg gtggtgtcga aatcgccgtg cgcgagtgct gcggttgtcc ggtccagaat
      301 ccgggtccat cgctcaatcg ctgcctgaag gtggtcgagc gcagaagcat caagaacacc
      361 gctctcccgg accatccgta cagcatccac atattcccgc gtccacccgg accgcgatcg
      421 gtgctctccg gccggcacgc catgctcttc cgatgcagag gcccagccga acccacgaac
      481 agggatgttg ttgatacgcg ccagcaggcg tccgacgtcc gcagccaacg caggtagcca
      541 aggggccacc tggatacctg ccagtgatct tcccgtcaga tgggacgtca aggtgatcga
      601 gcgatccaac tcaggtgggg cgacctcata gcgaatcaca ttgggaacag ggacgcctgc
      661 ggcgcgcagc aaccggtgag cggccacttc accgtcgcgc cgctcacgtg gctcctcccc
      721 aagccgcacc cagaactgct cctcaccaac cagcgcgcgg aagaccggcg tcgagacacc
      781 accctcggcg cgggagatca caagttcacc gtccgggaac catcgcgcca gtaccgcccg
      841 cacctgatca gggttcatgc ccgtggcaca cccaacaggc agggacgata gtcgctgata
      901 gacgcccacc ggaatccata cattagggac tcaatcgatg tggataaggg tttcaccggg
      961 acgccacggt cttcgcgcgg atcacgacag tgatctcgtt gcggttgtga tgcaactgcc
     1021 gggcaaagac aatctcccac gctcttgaga gcatccggaa cgtctcgtcg accacccgga
     1081 gtgggtccgt tggttgcagc ttcaccgtca tgataccgag gccatcaggc gccagatgcc
     1141 ctgccgccga attcatgacc gaggcggaaa gcaccggcgc catccgcatg tcgttcacga
     1201 ccaggtcgaa gcgctccctg ttctcggcaa gaaatggtcc ggcggtggtc ttcacatgat
     1261 ggaggccctg ccggttcgac aggcgcgcgt ccagctcacc gggatcgacc gcccagacct
     1321 cgaagcctcg ctccagcagg atgcgactcc agccgccggg actcgcgccc aaatcgagcg
     1381 ccgtcccgcc ctggggcagt gtcaccgcat cagtgcggaa gagttcctcc agcttgaact
     1441 cggcacgtga gatttgcccc ttcggcttcg cgagacgtac tcgcccgcca ggccaatcgg
     1501 tcagcgcgtt cgccgctccg ttcgacccaa agacgatccc ctgctccgtg acgcacgccg
     1561 agaggatatg cgcggctcca ccccgcttta cctcgatgtc catatcggtc agcgcagagg
     1621 caagacgccg ccagagctga tccgtgcgat acgccagatc cacctgtccg ctctgccaaa
     1681 cgtgaaggga aacttcgccg ggcagcggga actgctccca gagccccagc gcgatatcgc
     1741 cgagctctgc caggtcctgc gcctcttcca gctctgccat cccgacacca cgcatcaggt
     1801 ggcgcggaaa gacgaggtgc tgctcctgga tcgtccgcgc aatgagggag atggagacgc
     1861 cctcatcctc gaaggctccg agatccggac cgaccggctc catgaaggaa tcggggaagt
     1921 ggggccgcag ttcgtcagca gccgccgaga ggtaatcctc atcggcggag aaatagatgc
     1981 gagagatatc caaacgcgtg tgtccccaaa tcctgatacc tgatatagga gcgaacaggt
     2041 aatacaacgt atccgccgtc cttgatcgcg tagcgcgtcg tggtcctggc agattaccaa
     2101 ccagctcatt tcacttggtt cccgtgccag aagtttcgtc acttaaggtt cagtgaagat
     2161 tccctcgcgg agcgatggtc gctgccaccg tgtcccatct aggtcggggg ccctcgctgg
     2221 caagttacct cgcgtggacg acccaaggca aggattggtg gtagccaagg cgcttcgcgt
     2281 cggattttca tggccgagcc ctcagggtga aaaaggctgg ctttgcctca ggatgacgag
     2341 gttcttgaca agcggtccga accgcagcga tcgccgtcgt catcctgagc cgatgagtcg
     2401 agtgcaacga gacgaatcgt gtcgaaggat ctctgttcct ggcaaacgcg gcggtcacag
     2461 caagcaggga tcgctcgact gcgcccggaa tgacgatacc gggaaggctc cgacaggaca
     2521 cctgacagtt gatgacccgc ttgccatgac ctggcctgtc ggagaatgcc ctgactctcc
     2581 ggcctgtcag cagtgagcgg tattcccacc gttgccaccc ctcaccgtga atcgtggtac
     2641 aagctcctga gactcgtagc ctacgactca gccttgatcg gtcccagccc gtgcgaccca
     2701 aagacagcgt tgacgtagtc ggccacgtcc tgccgctcct cggtcccatc cagcaccatg
     2761 cattcgagca cgtccacgat ctcgggattt atctcatcca gctccttgac cacctggagc
     2821 tcgttccgtc tgggcgttgg atccgtcaca aagccataca tcgcatcggt gagatcgtcg
     2881 agatcgacct ggagtttgaa gaagtgtgtc atggcgtgaa atcctccatt tctgtcgtac
     2941 ccacatcata aagcaaggcg ggttccacca tcggctctgc cccggcgccc tggttactga
     3001 gaatcattct tgacaagtcc gccacagagg cgtatccatg gggcatggta tatgcgacgc
     3061 agcccatctg cgtcgcccgg ttgtctttat gaggatgcgg gcgaagatcc gctgcccgtt
     3121 caattccctc atattcgcga ctcaccgccc cacgaccaag gagtctttcc cgatggataa
     3181 caccaagccg gtgctgacca accgtcaggg ccacccgatt cataacaacc agcagatgag
     3241 caccgtcggc actcgcggcc cagcaacgct cgagaactat cacttcctgg agaagatcag
     3301 ccacttcgac cgcgagcgca ttccggagcg cgtcgttcat gctcgcggga ccgttgccca
     3361 cggtgagttc gaggcctacg ggacgattgg ggacgaacca gccagcacct atacgcgcgc
     3421 aaagctgttc caggagaagg gcaagaagac cccggtcagt gtccgcttct cgaccgttgc
     3481 aggtggccgg gactcctccg aggtggcacg cgacccgcgt ggcttcgcgg tgaagctgcg
     3541 taccgaggat ggcaactggg atctcgtcgg taataacctg cccgtcttct tcatccggga
     3601 cgccaccaag ttcccggatt tcatccattc ccaaaagcct gacccagtga gcaaccaggc
     3661 gaacgacccg cagcgcgcct tcgacttctg ggcggcctcg ccagaagcca tgcacatgct
     3721 gatgtggctc ttctccccgc gcggcatccc caagtcgtac cgccatcagg acggctttgg
     3781 cgtcaacacc taccgtatgg tcaatgcgaa gggcgagggc gtgctggtga aataccactg
     3841 gaaatcgcag cagggaatcg agtccctcac cgcagcacag gccgccgaga tccaggccaa
     3901 ggagcttggc agccacacca aggacctctt cgaggcgatc gagcgtggcg attatccgaa
     3961 gtgggaactc aatgtccaga tcatgagcga cgatgagcat ccagagctgg attttgatcc
     4021 tttggatgac accaagacgt ggccggagga tcaattcccg ctacgcccgg tcggtatgat
     4081 gacgctcaac cggaacgtca aggacaactt cgccgagaac gagcagatcg cgtttggcac
     4141 aggcgtgctc gtcgatggcc tcgatttctc tgacgacaag atgctggtcg gccgcacctt
     4201 ctcctacagc gatacccagc ggtaccgcgt tggcgcgaac tacctgcaat tgccggttaa
     4261 ccagcctgtg actgacgttc acacaaacca ggaaggcggc atgatgtcgt tcgggcacga
     4321 cgagcgcggc gccgacccgt cggtgaacta cgagccatcg gttcttgccg gtatggtgga
     4381 ggcagacgag tcctacaggg agtacacccc gtacgtcgaa ggccatatcg tacgggaatc
     4441 gatcgaccgc acgaacaact acgggcaggc aggcgagcgc taccgcacga tcgagcagtg
     4501 ggagcgcgac gacctggtcc tgaacatatc cacggggctg agccagtgtg caccaatcct
     4561 ccaggagaag atgatcgagc atttcaccag gtgtgataag gagcttggcc agagagtggc
     4621 cgagggcatc cgctcgatgt cggaatcaca gtcggaaaag gctccgatga caaccctggc
     4681 ataacgccag agcatcatag atacaaagag gcacgggctg gagcgtttcg ctccagcccg
     4741 tgcgatctcg tttcacgact cagctattcc cgcagatccg atcggtgatg aactgttcgg
     4801 cggcgtcgac atcaacatcc atcgccacac gtgccgtgga cggcgtttcc agacccggct
     4861 gccgagcaac cgtcatcccc gtcgtcaacc ggcccgccaa ctcgatatcc accaccaggt
     4921 cgtgccactg aagcaggtcc ggctggagaa ccgccgccgc ggcaagcgga tcgtgcaggg
     4981 atgtccgtcc ctcgcgctcc tggaaccccg gatagcgggc tacctgatcg gcaacggctt
     5041 catggaaagg cgttcccact gcctggatcg cctctacacc ctcctgtgtg atatccacct
     5101 tcagcgtcac atcgagagga acgagcgaga tgggagcccc cgaggcgaag acaacgcggg
     5161 ctgcttccgg atcacactgg atattgtgct ccgccgcacc acggacgccg gtccacgtcg
     5221 cgatcacacc acccatgatc gtgaggtgcg ccaggttcga cgccagtttc ggctcccgta
     5281 tcaatgccgc agccacgttc gtaagagggc cgacggccaa gagatgcacc ttgcccggcc
     5341 gcgccatgac actctcgatg agatagtcga ccgcgtgctg gtccggtgac tgcccgaaac
     5401 gcgcatcgtc tcctgcttcg agcagcccga caccttcatg ccctggccag tagaccggcc
     5461 ggttccgcat cagtggctcc gcaatgccct catacacagt gacgttgtcg cggccgcgga
     5521 gcgccagcag cttgcgcgcc atccgggcac ggagcgcgac gtcaccgtag accgtcgtga
     5581 cgccaagcag gtcgatctcc ggcgagccaa ggagcaccgc cagcgccagc gtgtcatcca
     5641 catcagtacc gatatcggtg tccagaatca ccggaacccg cgtatccacc attacctgca
     5701 tcccctccaa tgacccggtt cagacattca tccccggtat catcgcccac acgcaaggga
     5761 ttctccatgt cgagtttacg aacacgcagg tcgtagagag cagtgttaag tcgccggtca
     5821 aacaacacgg gccggggcta atgccccggc ccgtatcttc ccctattgat caccctggga
     5881 cacgataccg gtgcccatga cgggtcgtcc gctaggaagc gggcgtagcc tccggtgtac
     5941 caacggccgc aggaatgatg ttcgcgatgg agacaacgcc cggttcgtca ccgtcatttc
     6001 ggacctcccc ggtcgttccc ggggtgagca cgatgtactg ccccgcttcc agcatgaact
     6061 cggtattggc ttcgacactc tcgcccgctg cgtcgggcgt acctacctgg ccgattgtca
     6121 ccgggacgct gagcgtcatg gagagtgcac ctgactcgac ataggccagc tcgagcgccg
     6181 ggctttcagc aaccatgtac gtcacacctg gcgcgaaagt caggcggtag agcgacgggt
     6241 cctgccgtcc ctcggcaaat accaccgcat cggccgtgac gccgggggca atctcaaagg
     6301 cacctggggg aggtccgcca gcaggcggtg atgcatcctg agcaccggcg ccggagatga
     6361 gtccgaccaa ggcgatcagc cccagcacca ttaccgcaat agtgaacagg aaaccagaag
     6421 tacggcgcat gatctgctcc ttctcgtcca gaaccgcggc tgcggtcgcc ccccgaaaac
     6481 agattgtaaa aacctgcaat catcatatac agcgcgatgt gctgtaatca agagcttcgg
     6541 gacacattcc tagtggtgac ggagtgacga cactcgatac ggaaccggca cattcgcatc
     6601 ttcaaccgcg tgccgagtat cagctcgcgg tcccggacct gtgtgaagcg aacgtcaccc
     6661 cttacccacc tggtgggcgg gcgaccatca ggtggacaac accgagcaag cgggaatcat
     6721 cgcgctcaac ctgaaagcca aggtcgcgca gcatacggtc cgtcggccgg tcgatgttgc
     6781 aggccatgtg acgccgctgc agcggcgcga gcaccttctg ggccgtcccg acaatggggt
     6841 tcttcgccag cacatgctcg atgagcacga tcttccctcc cggctttacg acccgcgcca
     6901 tctcgtgaag tgccgatacc gggtccggca cggtgcagag caccaggctg gccgtgaccg
     6961 tatcgaaggt gtccgcggaa aatgctgcca ggctctccgc gttcatctgc cgcaatgtca
     7021 ccgggaacgg gctggactcc gcgtactttg aggcctccgc gagcatgccg gttgagacat
     7081 cgatgccggt gaaggatgcg atgggggcac ccaacgcctg cacccgacgc agggtctccc
     7141 cggtgccgac gccgatctcc aggacatccc cctccagcat ccgggcgaga cgatcacgat
     7201 aggccggccc gagcaccaga ttctcgcccc ggccctctcg ctcgtcccag gttggtgccc
     7261 gctcgttgta gattcttgag atccgctgga gatcgttcgg cgatcgatcc agtccaatcc
     7321 gtcgtaaccg gttcactcag atgtcctccc gctgcacgga cgtatgcgta tgatggagtg
     7381 tactgcc
//
LOCUS       DASYWM010000111         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_4238, whole genome shotgun sequence.
ACCESSION   DASYWM010000111 DASYWM010000000
VERSION     DASYWM010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4238"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VGR29_04570"
     CDS             complement(<1..455)
                     /locus_tag="VGR29_04570"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEV2072899.1"
                     /translation="MIYLPEHFDASDPGAMRELIDRFPLATLVTQGPEGISANHIPLI
                     LESRAGTCGTLIGHVARNNLVWRDGHHLGESLVIFQASDAYISPNWYPSKQETHEVVP
                     TWNYAVVHAYGPVIIHEDEKWLRGVVGKLTRKMEGITSERPWKMADAPN"
     gene            complement(467..1408)
                     /locus_tag="VGR29_04575"
     CDS             complement(467..1408)
                     /locus_tag="VGR29_04575"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV2072900.1"
                     /translation="MDDISPAKIDVVRAGLLAWWRANRRDLPWRHTRDPYRIMVSEIM
                     LQQTQVDRVIPYYHRWFEVFPTVQDLAAAPTAEVIRLWAGLGYNRRAVNMQRTAQAVV
                     ERGGEYPATIQELLKLPGIGPYTAGAIACFAFEQDVPFIDTNMRRVLHRLFIGVDVPA
                     PVASDKAITSLAAEVLPLGNGWNWNQGLIEFGALHCTARKPLCIVCPLQAECNAYPEI
                     QTAIVGNTKVARGRKQVPFEQTNRYFRGRIVDALRVHQDDGIPLSDLGPLLRPEFGES
                     DVPWLLEMVEGLERDGLAAIAEEEASYDVSETRVRLP"
     gene            complement(1588..2223)
                     /locus_tag="VGR29_04580"
     CDS             complement(1588..2223)
                     /locus_tag="VGR29_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEV2072901.1"
                     /translation="MAHDRQNDTYNIDVYTDYACPYAWASQAWLYQVQDVLGDRLQIT
                     WRAFPLQQVNAPDADFKVWELPNDGTSSTLRSFQAAHAAKLQGDDAFRGFHAQLFLRR
                     HEDGRNLAVQRVLEQTAEEAGLDLDRFREDLKSDKVFEIIRDDYTNGRQKLGVFGTPT
                     IVFENGQGAYLKLNFRDLPADPVEFWYDFVDTVRDRPTVIEIKRPDGPQRA"
     gene            complement(2250..2855)
                     /gene="coaE"
                     /locus_tag="VGR29_04585"
     CDS             complement(2250..2855)
                     /gene="coaE"
                     /locus_tag="VGR29_04585"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV2072902.1"
                     /translation="MTEQRRIVLGVTGNIASGKSTVVNILKEYGAHHIDSDLVYHDLV
                     QPGQPLLARLAEAYGDGIIAEDGTLDRKALGAIVFSDPEKLAELDCITHPAVIAESDR
                     RIAAIHSGVIVIDAVKLIESGHADICDEVWLVTAPEDVQIARLMRRNELTEEEARQRV
                     AAQPPLEPKIDRADRIIENSGSLGTLRATVEAAWYMLSKSN"
BASE COUNT          581 a          909 c          906 g          603 t
ORIGIN      
        1 tcgttcgggg cgtccgccat cttccatggg cgctccgacg tgattccttc catcttccgc
       61 gtcaacttgc cgaccacccc acgcagccac ttctcgtcct cgtggatgat aaccgggcca
      121 taggcgtgaa ccacggcgta gttccacgtc gggacgactt cgtgcgtctc ctgtttcgat
      181 ggataccagt tcggtgagat ataggcgtcg gacgcctgaa aaattacaag cgattcgccg
      241 aggtggtgcc cgtctcgcca aacaaggttg ttgcgggcaa cgtggcctat aagtgtgccg
      301 catgtgccgg cccgggactc caggatgagc ggaatgtggt tcgcggagat cccttccggt
      361 ccctgcgtga ccagtgtcgc cagggggaac cgatcgatca gttcgcgcat tgcgccagga
      421 tccgacgcgt caaaatgctc cggcaggtag atcatcggga cgctcctcaa ggcagccgaa
      481 cacgggtctc cgacacatca tacgacgcct cttcctcagc aatcgccgca agaccgtccc
      541 gctccaggcc ctcgaccatc tccagcaacc agggaacatc cgattctcca aactcagggc
      601 ggaggagggg gccgagatca gaaaggggaa tgccatcgtc ctggtgcacg cgtaaggcgt
      661 cgacgatgcg tccccggaaa taccggttcg tctgttcgaa cgggacctgt ttgcgacccc
      721 gcgcaacctt cgtattcccc acgatggctg tctggatctc cggataggcg ttgcattctg
      781 cctgcagggg gcagacgatg cagagaggct tgcgggcggt gcagtggagg gcaccgaact
      841 caatcaggcc ctggttccag ttccagccgt taccgagcgg caggacctcc gccgcaagcg
      901 atgtgatcgc cttatcgcta gctaccggtg ccgggacatc gacgccgatg aagagccgat
      961 gcagtacccg acgcatgttg gtatcgatga acggcacatc ctgctcgaat gcgaagcagg
     1021 caatggcccc ggccgtgtac ggcccgatgc cgggaagctt caggagttcc tggatagtcg
     1081 ccgggtattc accaccgcgc tcgacgacgg cctgtgcggt gcgctgcata ttgaccgcac
     1141 gacggttgta cccaagcccg gcccagaggc gaatgacctc agctgtcggt gccgcggcga
     1201 ggtcctgcac cgtcggaaag acctcaaacc agcgatggta gtaggggatg acgcggtcga
     1261 cctgcgtctg ctgaagcatg atctcggaga ccatgatccg gtacggatcg cgggtgtgtc
     1321 gccacggcag atcacggcgg ttggcacgcc accaagcgag aagccctgcc cggaccacat
     1381 caatttttgc aggcgagatg tcgtccatgg acccttccgg gtagtggaaa cgagagtgcc
     1441 gctcattagg tcgtcattgt gccggccggc aaaatcaacg cggttccttc tctggcatgt
     1501 ctcagtatgg cgacgaaagg acttaagtct cgcgcggccc tgcgaggctt gcagcgcatt
     1561 gcgttgcggc cggtggccct gcagtcgtta cgctcgctgg gggccgtccg ggcgcttgat
     1621 ctctatcacc gtcgggcggt cccgcactgt gtcaacgaag tcataccaga actccaccgg
     1681 atccgcggga agatcgcgga agttgagctt gagataagcg ccctggccgt tctcgaacac
     1741 gatcgtcggg gtgccaaaga caccgagttt ctggcggcca ttcgtgtagt catcacggat
     1801 gatctcgaaa accttatcgg atttcaggtc ctcgcggaac cggtccaggt cgagtccggc
     1861 ttcctccgcg gtctgctcaa ggacacgttg tacggcaaga ttacggccat cttcatgacg
     1921 cctgaggaag agctgcgcgt ggaacccccg gaatgcatcg tcaccctgca atttcgcggc
     1981 gtgcgctgcc tggaaggagc ggagcgtact gcttgtgcca tcgttcggca gctcccagac
     2041 cttgaagtct gcatccggcg cgttcacctg ctgcagcggg aaggcacgcc aggtaatctg
     2101 gagccggtca cccagcacat cctgcacctg gtatagccat gcctggcttg cccatgcgta
     2161 ggggcaggcg tagtcggtat agacatcaat gttgtacgta tcgttttgac ggtcgtgagc
     2221 cacgaatgga aacccttcat gtgtgaaagt cagttacttt tggaaagcat ataccaggcc
     2281 gcctcaaccg tcgcgcgaag cgtccccagc gaaccagagt tttcgatgat gcggtcggca
     2341 cggtcaatct ttggctccag cggaggctgg gcggcgacgc gttgccgggc ctcttcctca
     2401 gtcaactcgt ttcgccgcat caaacgagcg atctgcacgt cttcgggtgc agttaccaac
     2461 cagacctcgt cgcagatatc ggcgtgtccg ctttcgataa gcttcactgc gtcgataacg
     2521 atgacgccgg agtgaatagc cgctatccgc cggtcgcttt cggcgatgac ggcgggatgc
     2581 gtaatgcagt cgagttcggc cagcttctca ggatctgaaa agacaatggc gccaagcgcc
     2641 ttacgatcga gtgtgccatc ctcggcaatg atcccatcac catacgcctc tgcgagcctg
     2701 gcaagcaggg gctgaccagg ctgaaccagg tcgtgataga cgagatccga atcgatgtgg
     2761 tgggcaccgt actccttgag gatgttcacc acggtgctct tgccggacgc aatgttgccc
     2821 gtcaccccga ggacgattcg ccgctgctca gtcatgggcg acggccagtt cctgggcatg
     2881 atgggagatc gactcccatt ccgcgtaggc gccatccagc tcggtctgaa ccttctcgaa
     2941 ttcctcgccc agcttggaga ttgcggcaac gtcctggtcg atcgtggcaa tgcttagcc
//
LOCUS       DASYWM010000112         3450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_641894, whole genome shotgun sequence.
ACCESSION   DASYWM010000112 DASYWM010000000
VERSION     DASYWM010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641894"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VGR29_04590"
     CDS             complement(<1..437)
                     /locus_tag="VGR29_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072903.1"
                     /translation="MTVRTADTPTNGHRRGILVFLAIAFALSWLPFVPVLFGSEPAGG
                     MLMPLAPAIAAVVVRKWVTREGFRDSGLRPNLRHWRLYLIAAGWPILTTLLSVPLAIL
                     FGAAPDGFSIPWGIEGPGMLTLLLWLGISVVGAPIIFGEELGWR"
     gene            complement(604..>3450)
                     /locus_tag="VGR29_04595"
     CDS             complement(604..>3450)
                     /locus_tag="VGR29_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV2072904.1"
                     /translation="VRNIPRRRAQSTLIVIGLMLSTLIISAALGVGDTLQYSATSNVY
                     QQLGHVDALVLQSEEFDADMISGVLGTIDSSALAQTEAALAGNEDVDGIMPMLDVRVP
                     VMNEVAGQSEPNVILTGIDPTRLDQFNGLHSTNGGTIDLGALPAGHVVISEEAADTLN
                     AKIGDSLTVFYENTPSTLVVANITPNSSLAGQRETELSVVMPLDRLQELTNQPDTLTW
                     IAISTTGGVRNTDTDTATVVESLRPAFEDQDLGVAAVKNTSVQESEDLASLFSSFFLI
                     LGLFSVAAGVLLIVLIFTMLAAERRSEMGMARAIGTHRRQLIQQFIAEGSAYAILSGL
                     VGSALGVLATYGIAAAMGALFGDFFQIEPHVTVRSLVAAYCLGMVITFLAVAGSSWKV
                     SRLNVVAAVRDIPDVVTAMRKKSGLVWGILFLILGGLLTFQGLTGDTAFPFYTGMSLL
                     PLGVARLVRFFGAPSRPVYSLVGIALLTLWMLPQDIAENLFGTLEGDIEMFFLSGIFM
                     VIGATILIVHNTTFLLSVVSRAGGLFRSKLPAVRTAVAYPGAAPGRTGMTIAMFSLII
                     FSLVMMSTMNSNYSNMMLGDKANAGWDVRTDFYNANPITDFAATLDADGVATDEFAAV
                     GAVTSPSPFGSDVRLSDAEPWKQATVYGMDEGFITSSELTFQQHAEGYESDEAVLQAL
                     RTEPNVVVVDTWLLEVTENFGADESALVLTGLTSEDKTFAPYEIQLAQPDGTARTVTI
                     IGVIDSEISTLSGIFASQPTIDATYGTTATTSYYVALTDPDNATTVAQDIEASLMQNG
                     AQSVSIQDEMKESQEQEAGFLYLVQGFMGLGLFVGVAAVGVIAFRSVVERRQQIGVLR
                     ALGYQRNMVSLSFMIETAFIVGLGVLSGVVLGVVLSRNLFTSDAESSGVAFTVPWTQI
                     GIMMLATVTVALLMTWMPSRQASRIAPAEALRYE"
BASE COUNT          708 a         1046 c         1089 g          607 t
ORIGIN      
        1 cgccagccga gctcctcgcc gaagatgatc ggcgcaccga caaccgagat accgagccac
       61 agcaggaggg tcagcatgcc cggtccctct ataccccatg gtatcgagaa cccgtccggg
      121 gcggccccga acaggattgc cagcggcaca ctcaggagtg tggtcaggat cggccaaccg
      181 gcggcgatca ggtagagccg ccaatggcgg agattcggcc gcaacccgga atcccggaat
      241 ccctcgcgcg tcacccactt tcggaccacg accgccgcga tggcgggcgc gaggggcatc
      301 agcattccgc cagccggctc agagccgaag aggacgggga cgaacgggag ccatgagagc
      361 gcgaaggcga tcgccaggaa aacgaggatt ccccggcggt ggccgttcgt tggtgtatct
      421 gccgttcgaa cagtcatcac tcgtttcctt tctggagatg ggaggcggcg gagaaggcgg
      481 agcgcctcat tcaggggaat cccatgatcc ggggctgtag gcgagcgatt gagccctctg
      541 gaagaccctc aactgcgacg attgaggatg gcatcgttgc catcctcaat cgtctcgttc
      601 cccttactcg tatcgcagcg cctctgcagg ggcgatacgg gacgcttgcc gcgagggcat
      661 ccatgtcatc agcagtgcga ccgtgaccgt tgcgagcatc atgatgccga tctgcgtcca
      721 cggtaccgtg aaggcaacac cgctgctttc agcgtccgag gtgaagaggt tccgcgagag
      781 cacgacaccc aggacgacac cggagaggac accaagaccg acgatgaacg cggtctcgat
      841 catgaatgag agcgaaacca tgttgcgctg gtaaccgagt gcccgcagga cgccgatctg
      901 ctgccgtcgt tcgaccaccg accggaaggc aatgacaccg acggcggcga cgccgacgaa
      961 gagtcccaga cccatgaatc cctgcaccag gtagaggaac ccggcctcct gctcctgcga
     1021 ctccttcatc tcgtcctgga tcgagacgct ctgcgcgccg ttctgcatga gggacgcctc
     1081 gatatcctgt gcgacggttg tcgcattgtc cggatcggtg agtgcgacgt aatacgaagt
     1141 cgttgccgtc gtcccatagg tcgcgtcaat cgtcggctgg gatgcgaaga ttccgctcag
     1201 ggtgctgatc tcgctgtcga tgacgccgat gatggtcacc gtcctggccg tgccatctgg
     1261 ctgggccaac tggatctcgt acggggcgaa cgtcttgtct tcggacgtca gcccggtgag
     1321 aaccagggcg ctctcgtcag caccgaaatt ttccgttacc tccaggagcc acgtatcgac
     1381 gaccacgacg ttcggctctg tgcggagcgc ctggaggacg gcttcatccg actcgtaccc
     1441 ctcggcgtgc tgctggaatg tcagttcgct ggaggtgatg aaaccctcat ccataccgta
     1501 gacggttgct tgtttccagg gttccgcatc ggagagtcgt acgtcggaac cgaatggaga
     1561 cggactggta acggcgccaa ccgcggcaaa ttcgtcggtc gcaacgccgt ccgcatcgag
     1621 cgtcgcggcg aagtccgtaa tcggatttgc gttgtagaag tccgtgcgga cgtcccagcc
     1681 ggcattcgcc ttatctccga gcatcatgtt cgagtagttt gaattcatcg tcgacatcat
     1741 caccagcgag aagatgatga ggctgaacat ggcaatcgtc atccctgtcc ggccgggagc
     1801 ggcacctgga taggcaactg cggtacgtac cgccggcagc ttcgagcgaa agaggccacc
     1861 tgcccggctg acgaccgaga gcaggaacgt cgtgttgtgg acgatcagga tcgtcgcccc
     1921 aatcaccatg aagatgccgg agaggaagaa catctctatg tccccctcaa gcgtcccgaa
     1981 caggttctcc gcgatgtcct gcggcagcat ccacagtgtc aggagggcaa tgcccacgag
     2041 cgagtagacc ggccgtgacg gcgcaccgaa gaaccgaacg agtcgggcca cgccgagcgg
     2101 cagcaggctc atacccgtgt agaacgggaa tgcggtgtcg cccgtgagcc cctggaaggt
     2161 caacagacca ccgaggatca ggaacaggat gccccagacc aggccgctct tcttccgcat
     2221 tgcggtgacg acatccggga tgtcccgcac ggcggcgacg acattcaggc gactaacctt
     2281 ccaggacgag ccggcgacag ctaggaaggt gatgaccatg ccgaggcagt aggctgcaac
     2341 gaggctgcgc acggtgacgt gtggctcgat ctggaagaaa tccccgaaca gcgctcccat
     2401 cgcagctgca atgccgtagg tggcgaggac accaagagcg gagccaacaa gtcctgacag
     2461 gatcgcgtac gccgagccct ccgcgatgaa ctgctggatg agctggcgcc gatgcgtccc
     2521 aatggcgcgg gccatgccca tctccgagcg gcgctcggcg gccagcatcg tgaagatcag
     2581 gacgatcagg agcacgcccg ctgcgaccga gaagagcccg aggatcagga agaagcttga
     2641 gaagaggctg gcgaggtctt ccgattcctg gacagacgtg ttcttgaccg ccgcgacgcc
     2701 gagatcctga tcctcaaacg ctggccggag cgactcgacc accgtcgcgg tatcggtatc
     2761 ggtattgcgg accccacccg ttgtggatat cgcgatccat gtgagcgtat ctggctggtt
     2821 cgtcagttcc tggagacgat ccagcggcat gacgacacta agctcggtct cgcgctgtcc
     2881 ggcaagcgag gagttcggcg tgatattggc aacaaccagc gtgcttgggg tgttctcgta
     2941 gaacacagtc agggaatcgc cgatcttggc gttgagcgtg tcagcagcct cttcactgat
     3001 gacaacgtgc ccggccggga gtgccccaag atcgatcgtg cccccattgg tggagtgaag
     3061 gccgttgaac tggtcgaggc gcgtcgggtc aatgccggtc aggatgacgt tcggctccga
     3121 ctggcccgca acctcattca tcacggggac acggacatcg agcatcggca tgatgccgtc
     3181 gacgtcttca ttgccagcta gcgccgcctc ggtttgggcc agggcactgc tgtcaatcgt
     3241 gcccaacacc ccgctgatca tatcggcgtc gaattcctcg ctttgcagga cgagcgcgtc
     3301 gacatgaccc agttgctggt acacgttgga cgttgctgag tactgcaagg tatcgccgac
     3361 acccagtgcc gccgagatga tgagcgtcga gagcatgagc ccaataacga tgagggtgga
     3421 ctgtgcccgg cggcgcggaa tgttgcgaac
//
LOCUS       DASYWM010000113        11543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_172686, whole genome shotgun sequence.
ACCESSION   DASYWM010000113 DASYWM010000000
VERSION     DASYWM010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172686"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VGR29_04600"
     CDS             <1..880
                     /locus_tag="VGR29_04600"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEV2072905.1"
                     /translation="HPAFYGSFDWHSCVEMHWTAVRLLRLFPRPGTEDRARQTLSELL
                     TPENIAQEVAFFHDPLHRSFERPYGWGWLLMLWHELEIWDDPDGRYWVSTIAPLAQVI
                     MNHLGTWLPKMTYPQRIGMHSNTAFGLSLAWNAVERHRPDLLESIRSSTMRWFGEDKE
                     YPAHYEPSGADFLSAGLCEAELMSRVLPPELFPTWLERFLPGLANSEPPQIFTPAIVS
                     DNSDGQIAHLHGLNLSRAWAFIRLAQSLPAEDARIAPLISAAKTHADAAIGEVVASDY
                     MVEHWLAAYATLLLSE"
     gene            complement(943..1626)
                     /locus_tag="VGR29_04605"
     CDS             complement(943..1626)
                     /locus_tag="VGR29_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072906.1"
                     /translation="MIDGGIMGNLRRKLIDPFVIPLVIIAVTLALLTLIGESFLGVFT
                     PGQEVDRIDRPELWIGIGITVGVIALMAFLATRPAGSLGPLEKEVVIGSRPFDAKSLP
                     PVDSRVRSGEPGTINDIDAGYTLYASSGALATVRGLLPGGVDYGKRFAGFIYAEGKNG
                     VSDELWIPVEAVSAVYPQTRSAFLAIKGDETEAFGWNTPPETVNRGPVKHMPAAEKVK
                     DPTRFRQNQ"
     gene            complement(1690..2118)
                     /locus_tag="VGR29_04610"
     CDS             complement(1690..2118)
                     /locus_tag="VGR29_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072907.1"
                     /translation="MAVPLNAPSSPESERFLRSLEAERKRNAEERITGWTVVWTLFAF
                     KLGTIIIIWFAAGGSAEAKAYIAVTSWYWLGIPIAAMSGLIAYRWRLRKARRQVSQLR
                     QSEFSSRQDGDRSIRFSDEELREFMAMERRREELRRSGER"
     gene            complement(2160..3089)
                     /locus_tag="VGR29_04615"
     CDS             complement(2160..3089)
                     /locus_tag="VGR29_04615"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEV2072908.1"
                     /translation="MFSLLPLLPLFHVSGLEPTGPFYTHWILDPAVAVYIIGLTAAYF
                     AWVGPLNRRRPGAENRPVTRDHIRWFLLGQVLLLVALGPPIDDWAHFYFSSVHMLQHL
                     LLMFAVVPCWIKGTPPWVFDPLIRHRWGRYLLTYLPRALPAFLLASLIVVLWHIPAFY
                     NATLENELVHALQHQFFLITGFLFFWPLMSTVRESPQLSPPMKCVYLFLQTIPSGIVG
                     ATIVYAAPGLYPHYADASVRPWGLSVAEDQVIGGLLMWVGMNTVFLLLLSVIFLRWAN
                     AEQRRDREALRQGLMRQRIAPVAEPTGSRTINS"
     gene            complement(3207..3554)
                     /locus_tag="VGR29_04620"
     CDS             complement(3207..3554)
                     /locus_tag="VGR29_04620"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEV2072909.1"
                     /translation="MATPTVPAEHDATAHEHPSEFTYIKIAVILAIITAVEVLIYYIE
                     GLRGILVPALIALSAAKFLLVVGYFMHLKFDDKKLAWIFAFALITSVAVFVGVWLMQH
                     FARVQQFVGELIT"
     gene            complement(3613..4371)
                     /locus_tag="VGR29_04625"
     CDS             complement(3613..4371)
                     /locus_tag="VGR29_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEV2072910.1"
                     /translation="MTQAVEVVHASGGAGHAAGHGGDDHTSHTATGINHRKLAMWLFL
                     ASDCMFFGSLIATYMIYRGRAEEVFREGAGAGPFPSQLIDIPFTSVSAFVLLMSSLTM
                     VLSLAALQKNNLRGSRIWLAATAILGATFLGGQYFEFTEFYHEGLSLQTNMFGATFFT
                     LTGFHGLHVAIGVIWLLTLLYVSLRGGLKQKDAMSLEVAGLYWHFVDIVWIIIFTLVY
                     LVPYEEMGTEGLEEGGEHGMRLIEFGRNALGTFF"
     gene            complement(4414..6393)
                     /gene="ctaD"
                     /locus_tag="VGR29_04630"
     CDS             complement(4414..6393)
                     /gene="ctaD"
                     /locus_tag="VGR29_04630"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEV2072911.1"
                     /translation="MASIAQPHVQSHQATVQPVVHKQRSGLWDWLTTIDHKKIGILYG
                     VTAILFLMIGGLEAMLIRTQLVAADNTLIEANTYNALFTMHGTTMIFLGVMPLSVAFW
                     NYIMVLQIGARDVAFPRLNAFSYWLFLFGGIILNMSWFFGVNEVPNQGWYGYAPLTEE
                     AWSPTRAVDFWILSLQILGIASMLGAFNFIVTILNMRAPGMSLMRMPMFTWTTLITSI
                     LLIMAFPAITIALVFLMMDRWVGTGFYAPAAGGDPLLWQHLFWVFGHPEVYILILPAF
                     GVVSEIVPTFSRKPLFGYSVMVYATAAIAFLGFGVWAHHMFTTGLGPTANSVFAATTM
                     TIAIPTGVKIFNWLGTMTFGQIKFTTPMLFAVGMISQFTIGGISGVMHAVVPLDVHHN
                     DSYFVIAHFHYVLFGGSLFGLIAGIYYWYPKVTGRVMNESFGKWNFWIMFTGFNLTFF
                     PMHFLGLQGMPRRYYSYGEGSGWEFWNVVVSFGAFLLGFSMLMLLFNIGQSLRTGKRV
                     GANPWDAPTLEWATTSPPPVYNFREIPQVAHRDPLWAEKYGTDHEDEDSEVGITIAGQ
                     EVGTADVPDDKDKEALRLQQAEEEDAGHGIHMPNPSYYPLIAALGMFLLAVGMLIDFP
                     TLVALGPVDFSIVAVLGLVTLIGGIFGWSFEPASD"
     gene            complement(6553..7770)
                     /gene="coxB"
                     /locus_tag="VGR29_04635"
     CDS             complement(6553..7770)
                     /gene="coxB"
                     /locus_tag="VGR29_04635"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEV2072912.1"
                     /translation="MERHSQDRVEHTGQPRRRGMLSRLLTLGTLALMALLVLAGCGPD
                     IDPPYSHISPESPSTESIQDLYKLVFWLSLVVFVGVQFAVVYIAMRYRRKASITKRPP
                     QIHGSRRLEIAWTIIPAIVLLLLLIPTITLLYEGDAAADEGDIVIDVYGKQWWWEFQY
                     REDVTQEGQSLEVVTGNEITVPVGRELQLNLQSNNVIHSFWIPRLSGKLDLIPGHVNT
                     LSITPTEVGEYYGECAEFCGIQHAWMRFTINVVPEDEFYAWVNNWRTSPPTTVRGEAT
                     DQVVKAPDAFAVCLGCHRVNGVEGSVAPQGIEAPRSIGPNLTMLPCRETLAAGMLENT
                     PENLALWLSDPGAVKPGNYMADAIGPDTLTDEQIAELVEYLYSLEPEGGCTSAEGWTD
                     PGVAAEATPVPGS"
     gene            8380..8628
                     /locus_tag="VGR29_04640"
     CDS             8380..8628
                     /locus_tag="VGR29_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072913.1"
                     /translation="MQTPRSSPPGRRMMAIALIVGGAIITLLAVFADDVEIGTGRGFG
                     YYQMIVLIAGLVLLLGGGAILFQHRFSDRSGDDFEPEP"
     gene            8772..10997
                     /gene="argS"
                     /locus_tag="VGR29_04645"
     CDS             8772..10997
                     /gene="argS"
                     /locus_tag="VGR29_04645"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV2072914.1"
                     /translation="MANAPTNQTASSSLPGGIIAREERAAADAIRVALADAGVEPGAR
                     PIDLRPLPFEGTWGSASTIARMVAGELVTRELEASGALDGLSKKEMKRLVNEKVGDRA
                     QELAATVADSLRATGRFATVEAVNGYINISYDTTSMTASLVREVLTANTTYGHAAPGS
                     RVQKVMIEHSQLNTHKAAHVGHLRNICLGVAVTNISEAGGFTTMPVTYIGDIGRHVVR
                     CLWCYSAFHEGEEPEGGASRGRWLGEIYAESVNRLNFRKDVLSFLQRVTQEDEAFVEA
                     VDKMLKSLWREQVEGEDVAYLLGRITSQQEIKVEDLRDENVLVTFWPIIGEQLRAEAE
                     MAATAEDDARTPNDHLAEWEQLNTHMADWWPQVPTWIEQENATFQLWEKKDPAFVALW
                     EKTRGWSVDELKEIFDEFGAHFDAWFWESEIEDAGRQIVRELLERGIAEISDGLPVVK
                     IDERLGLEKETYHTLPILRSDGTTLYSTKDLALTQEKFEEYGVDQSIWVVDVRQSLYF
                     DQITKILELYGFEQAANSMHLSYEFVTLPTGVISSRKGNAPMLEDVRNTMLERARGVI
                     QEKNPSLPAEQQQQIAQDIAIGAIKYVMLARDGNKVIVFDPDEALSFDGHAAPYIQYA
                     HARACRILEHAGITDEAILGMIDSIESGQLQPEELSLLQQIAEFPGIVQRAADEHRPL
                     HITNYVYELARRFNDFYHACPVLQSEEPVRSARLALVTTTRITLRNGLALLGIAAPRQ
                     M"
     gene            11008..>11543
                     /locus_tag="VGR29_04650"
     CDS             11008..>11543
                     /locus_tag="VGR29_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEV2072915.1"
                     /translation="MTPSTVTTTRIAAANRLLTASIQPIAIPLWYGCEVPGVDRGALV
                     LRDGLQLRWISPQYSGLRDRLRPAITIPVAEEEAARERIDQRNMTFKSAIDDANADLA
                     NAVDTAIQHGHLALTLGGDHAVAMGSIGGGARHAANLGVLWIDTHPDLNTPESSFSGH
                     IHGMPLGTAIGLAPESMR"
BASE COUNT         2546 a         3518 c         3349 g         2130 t
ORIGIN      
        1 ccacccggcg ttctatggct ctttcgactg gcactcctgc gtggagatgc actggaccgc
       61 cgtgcgcctg cttcggctct ttccgcggcc cgggacggag gaccgtgccc gacaaacact
      121 aagtgaactg ctgacgccag aaaacatcgc gcaagaagtc gcattcttcc atgacccgct
      181 gcacagatcc ttcgagcgcc cctacggctg gggatggctc ctgatgctct ggcacgagct
      241 ggagatctgg gatgacccgg atgggcgcta ctgggtgtcc accattgcgc ccctggccca
      301 ggtcatcatg aatcatctcg gaacctggct tccgaagatg acctaccccc agcggatcgg
      361 catgcacagt aacacggcct tcggcctgtc gctcgcctgg aacgcggtgg agcgccaccg
      421 tccggacttg ctggagagca tccgctcgtc gacgatgcgc tggttcgggg aggacaagga
      481 gtacccggcc cactacgagc cgtcaggtgc cgacttcctc tccgcagggc tgtgcgaggc
      541 ggagctgatg tcccgtgtgc ttcccccaga attgttcccc acatggctgg agcgcttctt
      601 gccgggactt gccaactcgg aaccgccaca gatcttcact ccggcgatcg tctccgacaa
      661 ctcggacggg cagatcgccc acctgcacgg attgaacttg agccgtgcgt gggccttcat
      721 ccgcctcgct cagtcccttc ccgcagagga tgcccgtatc gcgccgctga tctcggcagc
      781 gaagacccat gcagatgccg cgattggaga ggtcgtcgcc agcgattaca tggtggagca
      841 ctggctggcc gcctacgcca cgctcctgct cagtgagtaa tgatgcgaaa cgggaacagt
      901 aagggccggg aagtttggcc cggcccttac ttattcgtcg ccttactggt tctggcggaa
      961 ccgcgttggg tccttgacct tctcggccgc cggcatgtgc ttgaccggac cgcggttcac
     1021 cgtctccgga ggagtattcc acccgaaggc ttcggtctcg tcgcccttga tcgcgaggaa
     1081 cgcggagcgg gtctgcgggt agacagcgga caccgcctcg acgggaatcc agagttcgtc
     1141 gctcacgcca ttcttaccct cggcatagat gaagccggca aagcgcttgc catagtcaac
     1201 gccaccgggc aacaggccgc gaacggtggc gagagcccca ctggaggcat agagggtgta
     1261 accggcatcg atatcgttga tcgttcccgg ctcaccagag cggacgcggg agtcaacagg
     1321 tggcagcgac ttggcatcga acgggcgaga gccaatcacg acctccttct ccagcgggcc
     1381 aagtgacccc gctgggcgtg tagctaggaa cgccatcagc gcaatgacac caacggtgat
     1441 accgatacca atccagagct caggacgatc tatccgatcc acttcttgcc caggcgtaaa
     1501 aacgccgagg aaactctccc cgataagtgt caacagggcc agggtcaccg cgatgatcac
     1561 gagcggtatc acaaatggat caatgagctt tcgtcgcagg ttccccataa tgcccccgtc
     1621 tatcacttca aaacgtacgt tccgccaagt gtcgggtgca ttggacgact tgtccaatgc
     1681 acggaatgat cacctctcgc cggagcgacg gagctcctca cgccggcgtt ccatcgccat
     1741 gaactcacgt aactcttcat cactaaatct gatcgatcga tcaccatcct ggcggctgct
     1801 aaattccgac tgcctgagct ggctcacctg ccgtcgtgcc ttcctcagcc tccaacgata
     1861 cgcaatcaag ccactcatcg ccgcaatggg aatccccagc cagtaccagc ttgtcaccgc
     1921 gatgtaggcc ttcgcctctg ctgacccacc agcggcgaac cagatgatga tgatagtccc
     1981 caacttaaag gcaaacaggg tccacaccac tgtccagccg gtgattcgct cctcggcatt
     2041 gcgtttccgt tccgcctcaa gagaccgcag gaaacgctcc gactccggtg atgagggtgc
     2101 attcagtgga acggccatag tgccctccct cgggttgctc gcgtctacgc gcccctagtt
     2161 tacgagttga tggtgcggct gccagttggt tccgcgacgg gcgcgatacg ctgccgcatg
     2221 agcccctgcc tcagtgcttc acgatccctg cgctgttcgg cattggccca gcgcaggaag
     2281 atcaccgaca agagcagcag aaaaaccgtg ttcatgccga cccacatcaa gagcccgccg
     2341 atgacctggt cttccgccac cgaaaggccc catggccgca cggatgcgtc tgcgtagtgt
     2401 ggatagagcc cgggagccgc atagacaatc gtcgccccga caatgccact cggaattgtc
     2461 tggaggaaaa gatagacgca tttcattggt ggtgacagct gcggcgactc ccgtactgtg
     2521 ctcatcagcg gccagaagaa gagaaatccg gtgatcagga agaactgatg ctgcaacgca
     2581 tggacgagct cgttctccag cgtcgcgttg tagaaggccg gaatatgcca cagcacgacg
     2641 atcagcgatg ccaacaggaa ggcaggcagc gccctcggca ggtaagtcag caggtatcta
     2701 ccccaccgat gcctgatgag cggatcgaag acccacggcg gcgttccctt gatccagcag
     2761 ggaaccacgg caaacatcag taacaggtgc tgcagcatat gcacgctgct gaagtagaag
     2821 tgcgcccagt catcgatcgg tggaccgagg gccaccagaa ggagcacctg acccaggagg
     2881 aaccagcgga tatgatcccg cgtgactggc cggttctcgg cgcccgggcg ccggcgattc
     2941 aacggcccta cccaggcgaa ataggcagcg gtgagcccga tgatatacac agcaaccgca
     3001 ggatcaagaa tccagtgggt atagaacgga cccgtaggct cgagcccgct aacgtggaaa
     3061 agtggcagca agggcagcaa tgaaaacatg aatgatcctg acgcttgtcc gcgccatgcc
     3121 gggaacatgt tcaacagcag ttgctcgttc cccaggtgac aatccgggcg accgacaaaa
     3181 catgccagtc gccgcagggt gttgtgttac gtgattagct cgccaacgaa ctgctgaaca
     3241 cgcgcaaagt gctgcatgag ccacacacca acgaacacag caacggacgt gatcagcgcg
     3301 aaggcgaaga tccaggctaa tttcttgtca tcgaacttca ggtgcatgaa gtatccaacg
     3361 accaacagga acttggcggc ggagagggca atcagggcgg ggacgagaat gccccgcaac
     3421 ccctcgatgt agtagatgag aacctctacc gccgtgatga tcgcgaggat aacggcaatc
     3481 ttgatgtagg tgaactccga tggatgctcg tgggcagtcg catcgtgttc ggcgggaacc
     3541 gttggggttg ccatgcatca actccatatc gcaagacccg gctacgactc cggacccgga
     3601 tgaatgcgtg cgttagaaaa aggtgccgag cgcgttgcgg ccaaactcaa tcagtcgcat
     3661 tccgtgctca ccgccctcct cgagaccttc cgtacccatc tcctcgtacg gaaccaggta
     3721 gacgagagtg aagataatga tccagacgat gtcgacgaaa tgccagtaga gaccggccac
     3781 ttcgagactc attgcatcct tctgcttgag cccgccccgg agcgagacat agagcaacgt
     3841 cagaagccag atcactccaa ttgccacgtg gagcccatgg aagcccgtca gcgtgaagaa
     3901 agtcgcgccg aacatattgg tctgcagtga gaggccctca tgatagaact ccgtgaactc
     3961 gaagtactgg ccacccagga acgtggcgcc gaggattgcc gtcgccgcca gccagatgcg
     4021 ggaaccgcgc aggttgttct tctgcagggc cgcgagtgag agcaccatcg tcagggaact
     4081 catgagcagg acgaaggcac tcactgatgt gaacggaata tcgatgagct ggcttggaaa
     4141 cggtcccgct cctgcccctt cgcggaagac ctcttcggca cgaccgcgat agatcatgta
     4201 ggtcgcaata agtgacccga agaacatgca gtcagatgcc aggaagagcc acatcgccag
     4261 cttgcgatgg ttgataccgg tcgcggtgtg cgacgtatga tcgtccccac cgtgccccgc
     4321 ggcgtgccct gctccaccgg aggcatgtac aacttctacc gcttgagtca tctaccaaat
     4381 cccctcaggt gacgcctgcc ccacgctgtt gtactaatcc gatgccggct cgaacgacca
     4441 gccgaagatc ccaccgatca aggtaacaag tcccaggacc gcgacgatgg agaaatcgac
     4501 aggtccaagc gcaaccagcg ttggaaaatc gataagcatg ccaacagcca ggaggaacat
     4561 gccaagcgcc gcaatgagcg gatagtagga agggttcggc atgtgaatac cgtggccggc
     4621 gtcctcctcc tcagcctgct ggagccggag tgcttccttg tccttgtcat cagggacgtc
     4681 cgcagtccca acctcctgac ccgcaatcgt gattccgacc tcggaatcct cgtcttcgtg
     4741 gtccgtaccg tacttttcgg cccacaaggg atcgcgatgc gcgacctggg gaatttcgcg
     4801 gaagttgtag accggcggcg gtgatgtcgt cgcccattca agcgtgggcg catcccaggg
     4861 gttcgcgccg actcgcttgc cggttcgcag tgactgtccg atgttgaaga gaagcatcag
     4921 cattgagaag ccaagcagga aggctccgaa cgaaacgacc acattccaga attcccaacc
     4981 ggagccctca ccgtagctat agtagcgccg cggcatgcct tgcaacccaa ggaagtgcat
     5041 cgggaagaag gtcaggttga agccggtgaa catgatccag aagttccact tgccaaagga
     5101 ctcgttcatc acccggccag tgaccttcgg gtaccagtaa taaatccccg cgatcaagcc
     5161 gaagagcgac ccaccgaaga gcacatagtg gaagtgggca atcacgaaat aggaatcgtt
     5221 atgatggacg tcaaggggca cgacggcgtg catgacgccc gagataccac cgatggtgaa
     5281 ctgggaaatc atacccacgg cgaagagcat cggcgtggtg aattttatct gcccgaatgt
     5341 catcgtcccg agccagttga agatcttgac accggtcggg atggcaatgg tcatcgtcgt
     5401 ggccgcgaac acagagttcg ccgtcggtcc caaaccagtg gtgaacatgt ggtgtgccca
     5461 cacaccgaag ccgaggaagg caatagccgc cgtcgcatag accataacgg aatagccgaa
     5521 caggggcttc cgggagaatg tcggaacgat ttcagagaca acaccaaacg cgggcaggat
     5581 cagaatgtac acctccggat gtccgaagac ccagaagaga tgctgccaaa gcaagggatc
     5641 gccaccggcg gccggagcat agaaccccgt gccgacccag cggtccatca tgaggaagac
     5701 cagggcgatg gtgattgccg ggaacgccat aataaggaga atcgaggtga tcagcgtggt
     5761 ccaggtgaac attggcatgc gcatgaggga cataccaggc gcgcgcatgt tcaggatagt
     5821 cacgatgaag ttgaacgccc ccaacatcga ggcgataccc aggatctgca ggctcaggat
     5881 ccagaagtca accgcccggg tggggctcca ggcctcctcg gtcagcggtg catagccata
     5941 ccatccctgg ttgggtactt cattgacgcc aaagaaccag ctcatgttca gtattatgcc
     6001 cccgaacagg aagagccagt aactgaacgc gttcaggcgg gggaaggcca catcgcgcgc
     6061 gccgatctgc agaaccataa tgtagttcca gaacgcgaca ctgagcggca taaccccaag
     6121 gaagatcatc gtggtgccat gcatcgtgaa caatgcgtta taggtgttgg cctctatgag
     6181 ggtgttatca gccgccacga gttgcgtacg gatgagcatg gcctcaagcc caccgatcat
     6241 caggaagagg atggcggtga ctccgtagag gatgccgatc ttcttgtgat cgatggtggt
     6301 gagccagtcc caaagaccgg atcgttgctt gtgaaccacc ggctgtacgg tcgcctgatg
     6361 tgactgcacg tgtggctgcg ctattgacgc catattgcgc tcctaccctt cgtacccagg
     6421 cgggtgttgg ttcacaccgc tcgcccgttc accattctgt acgaaaatcc gatgagacat
     6481 ccaagtgccg gtgccctgtg cgggaaagat gcaggcgcca gccggccaac gcctgctctc
     6541 gatgtgaacg ggctatgaac ccggcactgg ggtggcttcg gccgccacac ctggatccgt
     6601 ccagccctcc gcgctcgtac atccgccctc gggttccagc gagtagagat attccaccag
     6661 ttcggcgatc tgctcatccg tcagcgtgtc aggcccaatc gcgtcggcca tgtagttccc
     6721 gggtttgacc gcgcctggat cgctcagcca caaggcaaga ttttccggag tgttttccaa
     6781 catgccggct gcaagtgtct cccggcaggg aagcattgtg agattcggac cgattgaacg
     6841 tggggcttcg ataccctgcg gggccacaga accttccaca ccattcacgc ggtgacagcc
     6901 aaggcagacc gcaaaggcat ccggggcctt gaccacctgg tccgtagctt ccccccgaac
     6961 agtcgttggt ggagatgtcc gccagttgtt cacccaggcg taaaactcgt cctctggcac
     7021 aacattgatc gtaaagcgca tccacgcatg ctggataccg caaaattctg cgcactcgcc
     7081 atagtactca ccaacttcgg tcggtgtaat agaaagcgtg ttgacgtgcc ctggaatcaa
     7141 atccagcttg ccagaaaggc gtggtatcca gaaagagtga atcacattgt tcgactgcag
     7201 gttcagctgc agctcccgtc cgacgggcac ggtgatttca ttcccagtca cgacttccag
     7261 gctctgtcct tcctgggtga cgtcttcgcg gtactggaac tcccaccacc actgtttgcc
     7321 atagacgtcg atgacgatgt cgccctcgtc ggcagcggca tctccctcgt agagcaaggt
     7381 aattgtcggg attagcagca gaagcagcac aatcgccggg ataatggtcc aggcaatctc
     7441 aagacgcctg ctcccgtgga tctggggtgg acgcttggtt atcgatgcct tgcgccggta
     7501 ccgcatggcg atatacacca cagcgaactg gacgcccaca aagacgacaa gcgacagcca
     7561 gaaaaccagc ttgtacagat cctggataga ctccgtcgat ggtgattccg ggctgatgtg
     7621 cgaatagggt ggatcaatat ccgggccgca tcccgcaagc accagcaaag ccattagcgc
     7681 gagcgtaccg agtgtcagca gacggctgag catcccccgc cgacgtggct ggccggtgtg
     7741 ctctacccgg tcctggctat gccgttccat tctgcctccc cgcaccaaca tccctccccg
     7801 ggtgtggtga aactactcca aggcacgtag gggaatcacc agctacgagc cactcttcgc
     7861 cgactgataa cgattcgctc caccgatgat cagctcagca cgtgtctacg ccgatcgtgc
     7921 aagaaccgcc atacgagaga gccacccgta caaacgggtg ggggcctacg aggatacttt
     7981 accgcatcgg caattccgat tggggcatcg ctgcaagatc cccaacagca cgtccagtca
     8041 acgccaaagc cgatacccca cctcgtcgtg gtgcatgttc ctatacctgg acgttcacca
     8101 ccgaatcgcg actgcctggc cgtgtccggc aacgatacgt atagcgaagc cgtgcgggat
     8161 ggtcaagcac gatccgggcc aactcatgca cgggagcacc acaccttgcc cggtggtgaa
     8221 catgtcacct cacatgactg catggcagcc gggaagtcgc cattcaagga agttccggat
     8281 tcccctgctt cgcgtatact ttcgtcccaa tgggcaaacc gcacgccaca ggcaagcatg
     8341 accaatgctt cctgttgagt ggaatagatg gaccatccgg tgcagacacc tcgctcctca
     8401 cctcccggcc gacggatgat ggccatcgcc ctgatcgtgg gaggtgcgat catcacgctg
     8461 ctcgcggtct ttgcggatga tgtggagatc gggaccggcc ggggctttgg gtattaccag
     8521 atgatcgttc tcatcgcggg gctggtgctc ctcctcggtg gcggggcgat cctcttccag
     8581 caccgcttca gtgaccgctc cggcgacgac ttcgagccgg aaccctagta ccctccggag
     8641 aaccatccgt tttctcccga agcggtccaa cagggtatta ttacccggct tgagaatccg
     8701 cttgcctgcg cattcttgca ccaccgagac atcttccaca cctgttccgt atcaggagtc
     8761 acatcacctc catggccaat gcccctacca accaaacagc atcatccagc cttcccgggg
     8821 gcatcatcgc gcgcgaggag cgtgcggcgg ccgacgcgat tcgtgtcgcg ctggcggatg
     8881 ccggagtgga acccggagct cggccgatcg atctgcgtcc gctcccattc gagggaacgt
     8941 ggggaagcgc ctcgacaatc gcccgcatgg tcgccggtga gttggtgaca agagagctgg
     9001 aagcatcggg cgcgctggac ggcctctcaa aaaaggaaat gaagagactg gtcaacgaga
     9061 aggtaggtga ccgggcacag gaacttgctg caacggttgc tgattccctc cgcgcgaccg
     9121 gtcgtttcgc gacggtggaa gccgtcaacg gctacatcaa catctcctat gacaccactt
     9181 caatgaccgc cagccttgtg cgcgaggtcc tgacagcgaa cacgacctac ggtcatgccg
     9241 ccccgggttc gcgtgtgcag aaggtgatga tcgagcattc ccagctcaac acacacaagg
     9301 cggcccacgt cggtcatctg cgaaacatct gcctcggtgt cgccgtcacc aacatcagcg
     9361 aggctggcgg ttttaccacc atgccggtga cttatatcgg cgatatcggc cgtcatgtcg
     9421 tccgttgcct ctggtgctac agcgcctttc atgagggcga ggaacccgag gggggcgcct
     9481 cacgagggcg gtggctcggg gagatttacg ccgagtcagt gaatcgcctg aatttccgga
     9541 aggatgtcct gtccttcctt cagagggtca cccaggagga tgaggcattc gtggaggccg
     9601 tcgacaagat gctcaagagc ctctggcgtg agcaggtcga gggtgaggac gtcgcctatc
     9661 tccttgggcg catcacatcg caacaggaga tcaaggtcga ggacctccgc gacgagaacg
     9721 tgttggtcac cttctggccc atcatcggtg agcaactgcg ggctgaagcg gagatggcgg
     9781 cgacggccga ggacgatgcc agaacgccaa atgaccacct ggccgaatgg gagcagctca
     9841 acacccatat ggccgactgg tggccgcagg tacccacctg gattgagcag gaaaatgcga
     9901 ccttccagct gtgggagaag aaggaccctg cctttgtggc gctgtgggag aaaacccgtg
     9961 gctggagcgt cgacgagctc aaggagatct tcgacgagtt tggcgcacat ttcgatgcct
    10021 ggttctggga gagcgagatc gaggatgccg gacggcagat cgtccgcgag ctgctggagc
    10081 ggggtatcgc ggagatcagc gacggcttgc cggtcgtcaa gatcgatgag cggctgggat
    10141 tggagaagga aacctaccac accttgccga ttctccggtc ggatggaacg accctctact
    10201 ccacgaaaga cctcgcgttg acgcaggaaa agttcgagga atacggtgtc gatcagtcca
    10261 tctgggtggt cgatgtccgc caatcgctct acttcgacca gatcaccaag atcctggagc
    10321 tctacggctt cgagcaggcc gcgaacagta tgcacctgag ctacgagttc gtaacgctac
    10381 cgaccggcgt catatcgtcg cgcaagggca atgctccaat gctggaggat gtccggaaca
    10441 cgatgctgga gcgtgctcgt ggggtcatcc aggaaaagaa cccgtcgtta ccagcggagc
    10501 aacagcagca gatcgcgcag gatattgcga tcggggcgat caagtatgtg atgctggccc
    10561 gggatgggaa caaggtgatc gtcttcgatc ccgatgaagc gttgtcgttc gacggccacg
    10621 ccgcacccta cattcaatat gctcatgcgc gagcctgccg gattctcgag catgcgggca
    10681 tcacggacga agccatcctt ggcatgatcg acagcatcga atctggccag ctgcagcccg
    10741 aggagctcag cctgctgcag cagatcgcgg agttcccggg tatcgtgcag cgcgcggccg
    10801 acgagcatcg tcccctccat atcaccaact acgtctacga gctcgcgaga cgcttcaacg
    10861 acttttatca tgcgtgcccg gtgttgcagt cggaagaacc cgtccgtagc gcccggcttg
    10921 cacttgtgac aacgacacgt ataacgctgc gcaacgggct tgccctgctt ggaatcgcgg
    10981 cgccaagaca gatgtgattt tgaatagatg acaccttcca ccgtaacaac gacacgcatc
    11041 gccgcagcca accgtcttct caccgcatcc attcagccca tcgccatacc gctctggtac
    11101 ggatgtgagg tgcccggcgt tgaccggggc gccctcgtgc tacgcgacgg cctccagctc
    11161 cgctggatct ccccgcagta cagcggctta cgggatcgcc tgcgccccgc cattaccatc
    11221 ccggtcgcgg aggaggaggc cgcccgcgag cgcattgacc agcgaaacat gaccttcaag
    11281 tcagcaatcg acgatgccaa cgctgacctc gccaacgcgg ttgacaccgc gatccagcac
    11341 ggccacctgg cgctcaccct gggtggcgac catgccgtcg ccatgggtag catcggcggc
    11401 ggcgcccgtc atgcagcgaa tctgggtgtg ctctggatcg atacccatcc ggatctcaat
    11461 acccccgaga gcagcttcag cgggcatatc cacggcatgc cacttggcac cgcgatcggc
    11521 ctagcaccgg agagcatgcg gca
//
LOCUS       DASYWM010000114        22499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_417156, whole genome shotgun sequence.
ACCESSION   DASYWM010000114 DASYWM010000000
VERSION     DASYWM010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 22499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22499
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417156"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VGR29_04655"
     CDS             <1..544
                     /locus_tag="VGR29_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072916.1"
                     /translation="DHTNLRVNIHQFTKELCREERRTVGRLDSRFKGMDKDGVDDTPE
                     FDPSMAAIRPPFTSVLNTYFRTDLGYESDVEYHILRGLEWNWGDAMRGYSDTSDDLRA
                     AFAKNPFMKVFVASGYYDLATPYYATEYTLSHMNLDPEVRSNIHVEEFPTGHMVYLDF
                     KAMLRLKETVTSFINNAVDG"
     gene            complement(744..2192)
                     /locus_tag="VGR29_04660"
     CDS             complement(744..2192)
                     /locus_tag="VGR29_04660"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV2072917.1"
                     /translation="MTDFDQGLVADPRPDELDSEDLVRRPGETPGTALFTDLYHVDAA
                     YIAWRSGHNSQSTFDLYTRRAPFGGGFMLFAGLQPTLDYLDSFRYTEEDLGWLERVKG
                     YEREFLDYLAGLRFTGEIYAMDEGEIAFPNEPFLRVTAPFSEALLLESGLLRTIGIST
                     LLATKAARITLAANGRSVSDFAFRRAHAPFLAARSGYIGGCDSTSFVAAAREYDIPSA
                     GTIPHALVQAFPDELTAFRTVAESLPSYTLLLDTYDVDAGIDHAITVAQEAKASHGHT
                     LAAVRLDSGDLEADSRMVRRKLDEAGLETVKVLVSGDMDEYKIAELLVAGAPIDGFGV
                     GGNLGVGLGTIESGTVGGVIGAVYKLVWYEGDGAAPARIKLAGGKSTWPGRKMPYRIG
                     DHERDLITLDTEPAPKNARALLTPWVMEGQVIRRMPELKEIRANALANLHTLPDRLKA
                     LTPSEPYPVEWSEEMLRLREQTIRSLGGDPGA"
     gene            complement(2189..2923)
                     /locus_tag="VGR29_04665"
     CDS             complement(2189..2923)
                     /locus_tag="VGR29_04665"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEV2072918.1"
                     /translation="MPENQSDTPSFVNEWLTTLEPMSLAELVPEPERAAFFSADLING
                     FVHSGPLASPRVRGLVEPVADLFTSGWEHGIRHFVLLQDTHPQDTPEFRAYPPHAITG
                     TEESATIPELAALPFAGEFTVVEKNSLNPAIETGFDAWWDEHRGLNRAIIVGDCTDLC
                     VYQLAMHLRLRANALGLDDFEVIVPANTVDTFDIPVNAGTGPGSAHPGDFFHQVFLYH
                     MASNGIRIIRSLTSDSVDISTRSASE"
     gene            3116..3613
                     /locus_tag="VGR29_04670"
     CDS             3116..3613
                     /locus_tag="VGR29_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEV2072919.1"
                     /translation="MIHECPSCQVPRVFSVYETRRKLTLYFIPTLNVRSQQVMECTTC
                     HGRWGIPDDVRNEVDNRLMSQEQISEKIHREKAPPLQPPRAKTFYQVLQVDQEAERDV
                     IEAAFKRLAFRYHPDRSTSPESQGRMREILEARDILLDEVRRAAYDRSLGIVRRVEAL
                     RPEDV"
     gene            complement(3656..4258)
                     /locus_tag="VGR29_04675"
     CDS             complement(3656..4258)
                     /locus_tag="VGR29_04675"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV2072920.1"
                     /translation="MMIPENTCSISTKSSRLFYRKEKLRGACWEYEISGGWIQIPTGD
                     TVRHVLRHGFAFYRPREATIRTSRGTEIVRRAGAEQWFFPDRWFSLLRFTADDDRTIG
                     YYVNFSRPLQVVRENYYSDIDLELDLWLDLDGTLTELDRDEYEEEILAKRLAPEWAEA
                     VDAAARDVTLAVQQTIADFGPDVERTRDPLHGIPEFILRS"
     gene            complement(4255..5088)
                     /gene="rsmG"
                     /locus_tag="VGR29_04680"
     CDS             complement(4255..5088)
                     /gene="rsmG"
                     /locus_tag="VGR29_04680"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV2072921.1"
                     /translation="MTQNGIDITGSPEASAPRSDMPAFAALVRAVVPDLTDHQVSQLL
                     TYRDMLHARNQMVNLTAVRDFEGIERRLILESLRLYGKVKELAGEKHGRVMDLGSGGG
                     IPGMILAIALPEFTFSLLDATGKKVAFLRDCIDELGLTNTKAVHGRAEELAHAIEWRS
                     AYDVVTARAVTSLSALLELGLPFVQIKGWLVLPKGADIEDEIKPAAKAAAKLGGTIVE
                     ASFLPLVGSLVDTRLVLVRKDSPTPGTFPRRAGLPARAPLGSEQERPDKRVRRGKLKR
                     P"
     gene            complement(5098..7086)
                     /gene="dxs"
                     /locus_tag="VGR29_04685"
     CDS             complement(5098..7086)
                     /gene="dxs"
                     /locus_tag="VGR29_04685"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEV2072922.1"
                     /translation="MAASNGKISPHSPSPRSMPQGQVLSRIESPADLKGCTSRELSQL
                     AADVRAALISSVTETGGHLGASLGTVELTIALHYVFNSPLDKLVWDVGHQAYTHKLLT
                     GRKEAFGTLRQEGGISGFLSRDESEHDTFGAGHASTSISAALGMCVAEQEKSKGEPRG
                     HAVAVIGDGALTGGMAYEGLNNAGHMKYPLIVVLNDNEMSIAPNVGAMSKYLDRLRTD
                     PMYNRAKDELADFVEKLPKGERFVDFGKRVKDSMKEFIYHSALFEELGFTYIGPVNGH
                     HIPDLVSAFRHAAECNEPVFVHVLTQKGKGYDPASADIERSHAVSAPSLPKGKSSAPA
                     APKYQDVFAETVIELANSDDRIYAITAAMPSGTSLGKFKAKHPGRFFDVGIAEQHAVT
                     FAAGLATAGLRPVVGIYSTFLQRAYDQIIHDVCIQRLPVIFALDRAGFAGDDGRTHHG
                     VFDLSYLRCLPNMTIMAPRDENELRRMIRTAVDYTEGPVAIRYPRGAGLGVDTSGHPE
                     ALPIGKSEVLREGSDVALLAVGSMVEYARHAAQELANKGISATVVNARFVKPLDEELI
                     LTMADETGAIVTIEENSATGGFGSGVLELLSMHGKLVPTRVLGVPDRFFEQAPQTRLR
                     ELAGLGVDSIVAAARQVTEIRARTTIEVGSNIPASVSG"
     gene            complement(7298..7891)
                     /locus_tag="VGR29_04690"
     CDS             complement(7298..7891)
                     /locus_tag="VGR29_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2072923.1"
                     /translation="MADSTTPPDAEVVRRGELAELRRHQPADREHFMRWYQDPDIAEL
                     LRHDLRPLTPGQARGYFDSIVLPASSSGTAWAIHDRVSGNLLGTTAVTQINHTEGSCL
                     FRIVIGEKTAWGHGFGTDATKLVVEEAFETLGLTTIKLEVFEHNPRARRAYERVGFVE
                     TGRSVEWTRNHRLDVIAMELRRTIWEQSRDRSAHPAG"
     gene            complement(8069..8482)
                     /locus_tag="VGR29_04695"
     CDS             complement(8069..8482)
                     /locus_tag="VGR29_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEV2072924.1"
                     /translation="MSDRTLTCRDCNQEFTFTAGEQDFYTQRGFSDPQRCPECRSARK
                     TQRNQSGGSSYGGGGYSSGGYDSNDSGYSSGGGYRSGGGGGGYSSGPRTLYPAVCSEC
                     GKETEVPFNPTPGKPVFCRECFQSRKSSSPRYDSY"
     gene            8944..10086
                     /gene="sucC"
                     /locus_tag="VGR29_04700"
     CDS             8944..10086
                     /gene="sucC"
                     /locus_tag="VGR29_04700"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEV2072925.1"
                     /translation="MDVHEYQAAELLAKHGVPVNPGRVASTPEEAAAAAAELGGTVAV
                     KAQVHTGGRGKAGGIKLAHSPEDAKAAAEAILGMDIRGHTVNKVLVAKGADIAREFYL
                     GVVLDRPNRKVLIMASAEGGVDIEEVARTTPEKIVRVNADPALGFHSYQAKELGFALG
                     LPADKVNGFAKIAEQLYTAFIKEDATLVEVNPLILTGAGEWQALDSKMSFDDNALYRH
                     KDIEGLRDMAEENETELDARSSGISFVKLDGDIGCIVNGAGLAMATMDAVKLSGGEPA
                     NFLDVGGGASASQVAKAFSLVTSDPNVRAILINIFGGITRGDVVADGIREALGQVEVK
                     VPIVVRLSGTNADEGKKILAEAGLTAVDTMDEAAEQVVSAASGQAA"
     gene            10122..10994
                     /gene="sucD"
                     /locus_tag="VGR29_04705"
     CDS             10122..10994
                     /gene="sucD"
                     /locus_tag="VGR29_04705"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEV2072926.1"
                     /translation="MSILVGNDTRLLVQGITGREGSFHATQMVEYGTNVVAGVTPGKK
                     GESVAGVPVFDTVEEAVRETNPNTSIIYVPARFAPDAIYEAVDYGIQLVICITEGVPV
                     NDMVPVYHHVKQKGARLIGPNCPGLITPGEAKVGIMPGFIHEPGKVGLVSRSGTLTYE
                     VVDALTKANLGQSTAVGIGGDPVIGTSFVDVLELFQNDPDTEAIVMIGEIGGDDEETA
                     AAFIKEHVTKPMAGFIAGRTAPPGKRMGHAGAIISGGSGSAEEKIAALESAGVRVADS
                     PSKVPGLLKQAMNA"
     gene            11069..11608
                     /locus_tag="VGR29_04710"
     CDS             11069..11608
                     /locus_tag="VGR29_04710"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEV2072927.1"
                     /translation="MDGPELFDNGYMQLILGDIIRQRVDAIVNAANSSLLGGGGVDGA
                     IHRAAGPKLLEICRRIGGCPTGEARITPGFNLPAHYVVHTVGPIWRGGGHDEDRLLAS
                     CYRNSMRIAHESGVHSIAFPSISTGIYQFPLERAARIAVGELQEAAERYPEITVIRVV
                     AFSKETWDAYERAIEESID"
     gene            complement(11605..12237)
                     /locus_tag="VGR29_04715"
     CDS             complement(11605..12237)
                     /locus_tag="VGR29_04715"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2072928.1"
                     /translation="MTPDSTHAESDRRVTDARPLVTIEGDKIALGPIRRDLLPLYQAW
                     INDLSVTRFLVSRPMSLDEEISWYEGVVNNERMVFFTIYERSSYRPIGGVDLHGIDVR
                     NRTAELGIMIGERDARGRGLGTEAVRLVCDYGFNAMELNSIWLLTFGWNIAGQKAYVK
                     AGFREFGRRRQARYFDGRYWDDIYYDLLREEFESPVVHEIMSDGVSGNDR"
     gene            complement(12277..13041)
                     /locus_tag="VGR29_04720"
     CDS             complement(12277..13041)
                     /locus_tag="VGR29_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072929.1"
                     /translation="MATPLLERNASMRTFHALLILLFGAMLALAPLVSAQDAIDIPSI
                     AEVVLERDAESLVPALKAPIDEEGLPAAFSNAEYVDPASVSGDTGNIARGSMGDVVGT
                     VMYSIDYQPGTGASASPSPVASPAAGGPASIYNTASLNYLVYGDEVNPESLEGLGEVL
                     LSTIGDQAAEVEVSEITVDDTIAYVITISTVTNGIEVAMQWVAIPVGNVAVISMAMSG
                     GVNVDVDALRKDALALAIAGTRHLGAAAQDESQPAG"
     gene            complement(13434..13904)
                     /locus_tag="VGR29_04725"
     CDS             complement(13434..13904)
                     /locus_tag="VGR29_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072930.1"
                     /translation="MDLEPVAETVLDADQEALMKALETPIKDENLPEGFSNATFVDPE
                     TASAEEGVLPASDLEGAEGSVAYAIDFDPVARSVGATPGSSPAEAGFSIGLASLNYVF
                     MDDEITADDLEEFKSGAQEGLVGETGADASPEAGAPGMEATVEELGLRARRSPT"
     gene            14258..14821
                     /locus_tag="VGR29_04730"
     CDS             14258..14821
                     /locus_tag="VGR29_04730"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV2072931.1"
                     /translation="MQTSSRIFCPDCGARNDPTAHFCQSCGRSLEDVREVAAATNPVT
                     VGDEKDRSGRLLADDGLPIGEDLDGVPGGERVLWEGRPSILWSPRMALTQRYKLTNQR
                     LIMTFGFIGRRTEEIDLFRVNDVGVKQHPLERITRIGDVYISSADQSSPIRYLNNVKT
                     PDRVKDLLRESSRQERQRRRVLLREDV"
     gene            15004..16023
                     /locus_tag="VGR29_04735"
                     /pseudo
     CDS             15004..16023
                     /locus_tag="VGR29_04735"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
     gene            16190..17917
                     /locus_tag="VGR29_04740"
     CDS             16190..17917
                     /locus_tag="VGR29_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV2072932.1"
                     /translation="MEDIGLIIDLVTTLGIALVGGLIARRLGMPVLVGYILAGIVIGP
                     HTPGLVADGERVELMANLGVAFLMFALGVEFSLAQLLSVRKIALTTGAIQMPASVLLG
                     TGAGIALGWGIKSAILLGLAFLACSSIVVIKLTLSRGEATSPHARAALGLGILQDLSM
                     VPLLALLPMLKPDTTEVILPLAKSLLMAAAALVIVLVVGTRLVPKVFNFIAKSGTREL
                     FLLTIMVIALGTAYASHEAGLSYALGAFLAGLVVSESEFDAHVLAEIIPLRDVFSTLF
                     FVSLGMLLDPQLFLDHPLMIAVVVVVLVTGKFLASLAGYLLSGVGPVVATKAALLTAQ
                     IGEFSFVLASIGLSDAIIDDDQYSLILGIALASILLSPLLLMADPFISPVIQRLPYLR
                     DHMLSSVDREASYGEMQDHVIICGCGRVGEALAETLIRRQLQFVVIDMNPAVVQDLQS
                     RGIPALYGDSAAEPVLHRAGVEHARIVAVTVPNTIVALRTSRIAKELNPDLEIVVRAG
                     RAMEMQGLRVAGASDVVQPEFEAGLEFMQHVLRHQGVSAMETREIVDRRRHRFYQDDA
                     RALAFHDDA"
     gene            17961..18164
                     /locus_tag="VGR29_04745"
     CDS             17961..18164
                     /locus_tag="VGR29_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072933.1"
                     /translation="MTHDIHINTTPGEPDVDLTGKKLPVTGGSRGVGAEVARIGAVCG
                     ADVAINYRSKLRRAEQVAGKVRE"
     gene            18183..18740
                     /locus_tag="VGR29_04750"
                     /pseudo
     CDS             18183..18740
                     /locus_tag="VGR29_04750"
                     /inference="COORDINATES: protein motif:HMM:NF005868.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(18768..21992)
                     /locus_tag="VGR29_04755"
     CDS             complement(18768..21992)
                     /locus_tag="VGR29_04755"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2072934.1"
                     /translation="MDLPPAPYLVPLCAHLLGPVRIAVGNRVIPDHAWPRRSARALLL
                     LLLITPGHRLPRDRVLDLLWPERAPESAHGALRVALHTLRRVLEPDLRAGGESAYVES
                     RGDAVALRSGIALSIDLEVFEATAIRAGTAALADRPVMLHEALALFGGDLLEDEPYTD
                     WSAEPRERLRRTWRRAVLELAELELERCPLQSVPVLEKLLNADPTDEAAHRALMRALA
                     TAGRRDEALRQYARCVEELRDELDVEPDVETVALATEIRSAYQVPSLPMPPAAPAGRG
                     DNLSATPTPLIGRGREMESLQDILLHPDVQLVTVTGAGGIGKTRLALAVARQISDDFA
                     DGVCFVPLAAIHDPEVVLPMIAHTLEIKEVTGHPVAQLLGKALRERELLLILDNLEQV
                     IDVAEAIARLLEGCPHLTILATSREPLRVRAEHVVTIPPLAVPPLRLRTFPAPLLASS
                     VSRYEAVALFAERSRAARSDFAVTDANALAVAALCTRLDGMPLAIELAAALSSHLAPD
                     DLLARLARRLPLLTGGARDLPERQRTLRDAIAWSHDLLEPAEQTLFPRLAVFVGGFNL
                     DAAEAVCHALGDVIPEVASNVWALADKSLLQREEGTEGGSPRYRMLETIREFGLEKLK
                     ASGEEETIRRRHAAFFLATAENAEPLLHEMHQISWRDQLEVEHDNLRAALDWACEQQE
                     AETALGISGALAEFWRMGGHHTEGRMWLARALALTDSQPSAGRALCLRGAGVLAQAQG
                     DNDEAIARLTEALGDWRVLGDSRRTAQTLVPLAGIATARGEYVVALELNHQALRLFET
                     INDRAGIAGVLNQLGIIATDQGEYTRARGLYERSGSLYDLIGDRHGSARVLNNLGILA
                     FWQQDYRRAAMSFEDSLIHWRALGDRPHTAVALANLGEALRAEGDLEHAVTVAREGLQ
                     ISREVGDKRSAATALFILGSLLQHHEDNPQATEPLVEGLLLYQQIGDRLGMAWCLEAL
                     AGPASVSERPEVAAQMGGAAEVLREQVGVPLKPAELPAYRRHMDAARHAFSNPDAYHV
                     AWTTGREMALNTVVELAAGLLPDPVRSPEPSQASEREPIAGNKA"
     gene            complement(22241..>22499)
                     /locus_tag="VGR29_04760"
     CDS             complement(22241..>22499)
                     /locus_tag="VGR29_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2851 domain-containing protein"
                     /protein_id="HEV2072935.1"
                     /translation="FALAWAAHSEDAGLEDAASQAWVSLTHAEWTRPAKRALRQVTGG
                     PSLRDLGERGHQGLLHLDRTLCTPGRCYECPIAAEVIRDQL"
BASE COUNT         4322 a         6663 c         7143 g         4371 t
ORIGIN      
        1 tgaccatacc aacctgaggg tcaacattca tcagttcacg aaagaactct gtcgtgagga
       61 gaggcggact gtggggcgtc tcgacagccg cttcaagggc atggacaagg acggggtcga
      121 cgacacgcca gagttcgatc ccagcatggc agcgatccgc ccgcctttca cctcggtgct
      181 gaatacctac ttccgcaccg acctcgggta tgagagcgac gtggagtacc acatcctgcg
      241 cggcctggaa tggaactggg gtgacgcaat gcgcggatac tccgacacgt cagacgatct
      301 tcgcgccgcg ttcgcgaaga atccctttat gaaggtcttc gtcgcctccg gctactacga
      361 tctcgcgacc ccgtactacg cgaccgagta cacgctcagc catatgaacc tcgatcctga
      421 ggttcgatca aatatccatg tcgaggagtt tccgactggc cacatggtct atcttgattt
      481 caaggcgatg ctgcgactga aggagacagt cacttcattc atcaacaacg cggttgacgg
      541 gtaggtgggc accctgcgga ggtgacacgt agggtgaaag ggtcgctggt tgggcctggt
      601 ccgaggttca gtgactctac ggtccacccg gcttctgtac tgccgattcg gggccccggg
      661 ctaacactgc gtggcaggta cagcccctac acgcagccag cgatggtcac gggcggattg
      721 ccggtgaccg tcgccggtcg ttcttatgcg cccgggtcgc cgccgaggga gcggatcgtt
      781 tgctcgcgca acctgagcat ctcctcgctc cattccaccg ggtagggttc gctcggcgtc
      841 agcgccttca gtctgtcagg cagggtgtgg agattcgcga gagcgttcgc acgaatctcc
      901 tttagctccg gcatgcggcg aatcacctgg ccctccatca cccatggagt caggagtgca
      961 cgagcgttct ttggcgcggg ttccgtatcc agggtgatca ggtccctctc atggtcgccg
     1021 atgcggtagg gcatttttcg cccgggccac gtgctcttgc ctcctgccag cttgatccgc
     1081 gccggagcgg cgccatcgcc ttcgtaccag accagcttgt acaccgcacc gatgacgccg
     1141 ccgacggtcc cgctctcgat ggttcccaaa cccacgccga ggttgccgcc cacaccgaat
     1201 ccgtcgattg gagctccggc taccagcaac tctgcgatct tgtactcgtc catgtctccg
     1261 cttacgagca ccttcaccgt ttccagcccc gcctcgtcca gttttcgccg gaccatacgg
     1321 ctgtccgcct cgaggtcgcc actatcgaga cgaacggccg cgagcgtatg cccgtgcgaa
     1381 gccttggctt cctgtgccac ggtgatcgcg tggtctatgc ccgcgtctac atcgtacgta
     1441 tcgaggagca gcgtgtatga aggcaaagat tccgccaccg tccggaaggc cgtcagttcg
     1501 tcaggaaatg cctgcacgag tgcgtgcggg atcgttccgg cggaggggat gtcgtattcc
     1561 ctggcggcag cgacgaagga tgtgctgtcg cagccaccaa tgtagcccga gcgtgccgcc
     1621 agaaagggag catgagcccg gcgaaaggcg aaatcgctga cagagcggcc atttgctgcc
     1681 agggtgatac gggcggcctt ggtggccagt agcgtggaaa tgccgatcgt ccgcaggagg
     1741 ccggactcca gcagcagagc ttcggaaaat ggcgcggtta ctcgcaggaa aggctcgtta
     1801 ggaaaggcga tctcaccttc atccatagcg tagatctcgc cggtgaagcg gagacctgcg
     1861 aggtaatcaa ggaactcccg ctcgtagcct ttgacgcgct cgagccagcc aaggtcttcc
     1921 tcggtatacc tgaacgagtc gagataatcc aacgtcggct gcaagccggc gaagagcatg
     1981 aagcccccgc cgaatggtgc ccgccgcgta tagaggtcga aggtcgattg gctattatgg
     2041 ccagatctcc aggcgatgta ggcggcgtcg acgtgataga gatcggtgaa gagggccgtt
     2101 ccgggagttt cacccggccg tcggacaagg tcttccgaat cgagttcgtc cggccgggga
     2161 tcggcgacga gcccctgatc aaagtcggtc attcgcttgc cgatcgtgtc gagatgtcca
     2221 cactgtcaga cgtcagcgat cggatgatcc ggatgccgtt gctggccatg tgatagagga
     2281 agacctggtg gaagaagtca ccggggtggg ctgagccagg gccagtaccg gcattcaccg
     2341 ggatatcgaa ggtgtctact gtgtttgcag gaacgatcac ctcgaagtca tccaatccga
     2401 gggcgttggc gcggagccga aggtgcatcg cgagttgata gacgcagaga tcggtgcagt
     2461 caccgacgat gattgcccga ttgaggcccc ggtgctcatc ccaccacgcg tcgaagccgg
     2521 tctcgattgc ggggttcagc gaattcttct cgactactgt gaactccccg gcgaaaggca
     2581 gtgcggcgag ctccgggatc gtcgcagatt cctccgtacc ggtgatcgcg tgcggcggat
     2641 aggccctgaa ctccggggtg tcctgcgggt gagtgtcctg gagaaggacg aagtggcgaa
     2701 tgccgtgctc ccagccactg gtgaagagat cggccaccgg ttcgaccaac ccccgcaccc
     2761 ggggcgacgc gagcgggcca ctgtgaacga acccgttgat caggtcggcg gagaagaacg
     2821 cggcccgctc cggctcgggt acgagttcgg cgagcgacat cggttcgagg gtggttagcc
     2881 attcgttcac gaacgacggt gtatctgatt gattctcagg catacagtgg tccctccggc
     2941 ccaggcaatt cacgcatcgg gccttgtttt cgcacggacg atacctccat gaggcgcttg
     3001 acccccctgc taaagtgaag gcgaagcaaa tgagccggca gtgaccggtc cggagtgtga
     3061 ttgaccagtg ctacccatta ttggtgccag agactatatc gacccacgtg gattcatgat
     3121 tcacgagtgc ccatcgtgcc aggtaccacg ggtattttca gtttacgaga cccggcgaaa
     3181 gctgacgctc tacttcatcc cgacgctcaa cgtgcgctcg cagcaggtga tggaatgcac
     3241 cacctgtcac ggccggtggg gcattcccga cgacgtgcgc aacgaagtcg acaaccggct
     3301 gatgtcgcag gagcagatat ccgagaagat tcaccgcgag aaggcgccac cgctgcagcc
     3361 tcctcgcgca aaaaccttct atcaggtgtt gcaggtggat caggaggcag agcgcgacgt
     3421 gatcgaagcg gccttcaaac gtctcgcgtt caggtatcac ccggacagat caacttcacc
     3481 ggagtcccaa ggcaggatgc gcgagatcct ggaggcacga gacatcctgc tggacgaggt
     3541 tcggcgggcg gcctacgacc ggtcgctcgg gattgtgcgg cgcgtcgaag ccctgcgtcc
     3601 ggaggatgtc taggtccagc ttcccggcag ttcttccggc acttggaggg gctgttcagg
     3661 agcgcaggat aaactccggg ataccgtgga gggggtcgcg agtgcgttcg acatcgggcc
     3721 cgaaatcggc gatcgtctgc tgaacggcca gggtgacatc gcgggcagct gcatcgacgg
     3781 cttcggccca ttccggcgcg agtcgcttcg ccagaatctc ttcctcatac tcgtcacgat
     3841 ccagctcggt cagggtgccg tcgaggtcaa gccagagatc gagctcaaga tcgatgtcgc
     3901 tgtagtagtt ctcccgaaca acctgaaggg gccgtgagaa gttcacgtaa tagccgatcg
     3961 tgcggtcgtc atcggcggtg aaccggagca acgagaacca gcgatcaggg aagaaccatt
     4021 gctcggcccc ggcgcgacgg acaatctcag tgccacggga ggtccgaatc gtcgcctcac
     4081 gtggacggta gaaggcgaat ccgtgacgga gcacgtggcg caccgtatca cccgtgggaa
     4141 tctggatcca gccacctgaa atctcgtatt cccaacatgc cccgcggagt ttctccttgc
     4201 ggtagaacaa cctgctcgac ttggttgata tcgagcacgt gttctcgggg atcatcatgg
     4261 tcgtttcagt ttgccgcgcc gcacccgttt gtccggacgc tcctgttcgc tgccgagtgg
     4321 cgctctggcg ggtagtcccg ccctgcgggg aaaggttcca ggtgtggggg aatctttccg
     4381 aaccagcacc agccgcgtat ccactaatga tcctaccaag ggcaagaacg atgcctcaac
     4441 gatcgttccg cccaatttgg cagcggcttt cgcggctggc ttgatctcgt cttcaatgtc
     4501 cgcgcccttt ggcagcacga gccacccctt gatctggacg aaggggagac cgagctccag
     4561 gagagcggac agggaggtga cggcacgggc tgtcacgaca tcgtaggcgc tacgccattc
     4621 gattgcgtgg gccagttctt cggcgcggcc gtgaaccgcc tttgtattgg tgaggccaag
     4681 ctcatcgatg cagtcccgaa ggaatgcaac tttttttcct gtggcgtcga gcagggagaa
     4741 ggtgaactcg ggcaacgcaa tagcgaggat cattccgggt atgccaccgc cgctgccgag
     4801 atccatgacc cggccatgct tttcgcccgc gagctccttg accttaccgt acaggcgcag
     4861 gctctccagg atcaggcgcc gctcgatgcc ctcgaaatcc cggaccgctg tgagattcac
     4921 catctggttt cgggcgtgca acatatcccg ataggtcaaa agttgactga cctgatggtc
     4981 ggtcagatcg ggaacgaccg cgcgcacgag ggctgcgaac gccggcatgt cgctacgtgg
     5041 agcagatgct tcaggggaac cggtgatatc gataccgttc tgggtcatca ggtccgatca
     5101 gcccgaaaca gacgcaggga tattggaccc gacttcgata gtcgtgcgtg cgcgtatctc
     5161 agtaacttgc ctggctgcgg ccacgatgct gtcgacgcca agaccggcca gttcccgcag
     5221 gcgcgtctgg ggggcctgct caaagaaccg atccgggact cccagcaccc ttgtcggcac
     5281 caacttcccg tgcatggaca ggagctcaag gacgccggaa ccgaatccgc cagtagcaga
     5341 gttttcttca atcgtcacga tggcaccggt ttcgtccgcc atcgtgagga tgagctcctc
     5401 gtcgagtggt ttgacgaacc tggcattcac cacggtggcg ctgattccct tgttcgccag
     5461 ttcctgggcg gcatgccggg cgtactccac catcgatcca acggccaaaa gcgccacgtc
     5521 gctgccttcg cgaagcacct cactcttgcc gatgggaagt gcctcgggat ggcccgaggt
     5581 gtccacgccg agtccggctc cgcggggata tcggatcgcc accggccctt cggtgtaatc
     5641 aacggcggtc ctgatcatcc ggcgcagctc gttttcgtcc ctgggcgcca tgatcgtcat
     5701 gttgggtaaa cagcggagat agctgaggtc gaagacacca tggtgggtgc ggccgtcatc
     5761 accggcgaag ccagcacggt cgagtgcaaa gatgaccggc aatcgctgga tacagacatc
     5821 gtggatgatc tggtcgtagg cccgctgcag gaaggtcgag tagatgccta cgacggggcg
     5881 aagaccagcg gttgccaacc cagcggcgaa agtgacagca tgctgttctg cgatgccgac
     5941 atcgaagaag cgtcccggat gcttcgcctt gaacttcccg agagaggtgc ctgagggcat
     6001 agcagcagtg atggcgtaga tccggtcatc gctgtttgcc agctcgatca ccgtctcggc
     6061 gaagacatcc tgatacttgg gtgcggccgg cgcgctgctc ttgcccttgg gtaaggacgg
     6121 tgcgctcacg gcatgactgc gctcaatatc ggccgatgcg ggatcgtacc ccttgccctt
     6181 ctgggtgagc acgtgcacga atacaggctc gttgcactcg gctgcatgac ggaaagcgga
     6241 gaccaagtcg ggaatatggt ggccgttgac cggaccgatg taggtgaagc cgagttcctc
     6301 aaagagcgcc gagtgataga tgaactcctt catcgaatcc tttacacgct tgccgaaatc
     6361 aacgaaccgt tcgccctttg gcagcttctc aacgaagtca gccagctcgt ccttggcccg
     6421 gttgtacatt ggatcggtac gaaggcgatc gaggtacttg ctcatcgcgc cgacattggg
     6481 ggcgatagac atctcgttgt cgttcagcac gacgatgagc gggtacttca tgtgcccagc
     6541 attgttgagc ccctcgtacg ccatgccgcc ggtgagcgcg ccatcaccga tgacggcgac
     6601 ggcgtgcccc cgtggttctc ccttagactt ttcctgctcg gcgacgcaca tcccgagcgc
     6661 ggcggagatt gaggtggaag catggccggc accgaaggtg tcgtgctcgc tctcgtcgcg
     6721 gctcaggaag ccagagatcc cgccctcctg ccggagcgtc ccgaaggctt ccttgcggcc
     6781 ggtaaggagc ttgtgtgtat aggcttggtg gccaacatcc cagaccagct tgtccagcgg
     6841 cgagttgaag acatagtgga gggcgatggt cagctcaaca gtaccaaggc ttgctcccag
     6901 atgaccgcct gtctccgtga ccgaactgat aagcgcagcc cgcacatcag cggcgagttg
     6961 gctcagttcc cgggaggtgc accccttgag atccgccggg gactcaatgc gggagaggac
     7021 ttggccttga ggcatggaac gtggggatgg ggaatgcggc gagatcttgc cattgctggc
     7081 tgccataatg ctcttcctta ctgaatattg gcccgggatc aggtcaagcc ggccgcgatg
     7141 gtcgggcata cgcgcgcttt agttcggaca tgatggtgaa gagcgcatgc ggcctgccat
     7201 gatatatgag gtcactctac aaacccgtcc ggtgtcatgc aaaggtgcac gaaaaaggac
     7261 ccggtgtcgg gcgagcctat ggaagtatac gtctgtttca ccccgcggga tgagcggatc
     7321 tgtcccggga ctgctcccag atggtacgac gcagctccat agcaatcacg tcgaggcgat
     7381 gattacgcgt ccattcgacg ctgcgacctg tttctacaaa cccgacgcgc tcataggcgc
     7441 gacgcgcgcg cggattatgc tcaaaaactt ccagcttgat cgtagtgaga cccagcgttt
     7501 cgaaggcttc ctcgaccaca agctttgtag cgtcggtgcc aaagccatgg ccccacgcgg
     7561 tcttttcccc aataacaatg cggaagaggc aggatccctc agtgtgattg atctgggtca
     7621 cggcggtcgt gccgaggaga ttgcccgaga cgcggtcgtg aatcgcccag gcggttcccg
     7681 aagatgaggc cgggaggacg attgagtcga agtagcctct tgcctgaccc ggagtaaggg
     7741 gacgtagatc atgccgcagc agctcggcaa tgtcagggtc ctggtaccac cgcatgaaat
     7801 gctcgcgatc agcaggttga tgccggcgca gctcggccag ctcgccccta cgtacgacct
     7861 ctgcgtctgg tggtgtagtg gagtcggcca tagtacgttc ctggtccgtg cgaagaacat
     7921 gggagacgat acgacggatc atgacgatgg cgccctggag gatgtgggca agaaggatgc
     7981 ccatgatgat ggaatgaaaa atcgggccgg gggatgattc ccctggcccg attgtatcgt
     8041 ctgttctcag ttgagatacg gtgatgtcct agtaggaatc ataccgtggg gacgagctct
     8101 tgcgagactg gaagcactcg cggcaaaaga ctggcttgcc aggggtcgga ttgaagggga
     8161 cttccgtctc cttaccgcac tccgagcaga ctgccggata cagcgtgcgc gggccggagg
     8221 agtatccacc gccaccacca ccgctgcgat agccaccgcc agaggagtat ccgctgtcgt
     8281 tgctgtcgta accacctgag gagtatccgc ctccgccgta gctgctgcca cccgactggt
     8341 tgcgctgggt cttgcgtgcg gagcgacact ccgggcaacg ctgaggatct gaaaagcccc
     8401 gctgcgtgta gaaatcctgc tcacccgccg tgaacgtgaa ttcctgattg caatcgcggc
     8461 aagtcagcgt tcgatcactc atgttccgcc tgaacctctc ttgtcgggcg tacttcccga
     8521 cacaaactat gaaaaaagat atgtcgagtc gcttccgaac ggacggaacg cgaaatggcc
     8581 gactgagacc gtttcgagcg caacctactg gagacgtctc ggttcttgca gtatagcagg
     8641 aacgaaatga aaaaggccac tcattccgaa tattaggtat gaagctacct gtgatcgata
     8701 aagcagggac ctcacgaatg gagttctgcc tgaccaagtg acgctaccca tcttcatttg
     8761 ctaatttttt cacgatcttt tgagcgatga aacgggggcg tggatcgtcc tgaaccatgc
     8821 tttgcgtgtg atacgatcat tgatcgaaag acgcggatca ggctggaatc cgggacaggc
     8881 tcacccgatg gacgtggtgc gaaccgggga ccgtttaggc aggcataagg agcagcacgg
     8941 gctatggatg tccatgagta ccaggcagca gaacttcttg cgaagcacgg cgtaccagtc
     9001 aatcccggcc gggtggcctc gacacccgaa gaagcggccg cggcagcagc agagcttgga
     9061 ggcacggtag cggtcaaggc ccaggtgcac actggaggtc ggggaaaggc gggcggcatc
     9121 aagctggccc atagtcccga ggacgcgaaa gcggccgcgg aggcgattct cggtatggac
     9181 atccggggtc acaccgtgaa taaggtgctg gtagcgaagg gggccgacat tgccagggag
     9241 ttctatctcg gcgtcgtgct ggaccgccct aatcgcaagg tcctgatcat ggccagcgcc
     9301 gaaggaggcg tggacatcga ggaggtggcc aggaccacgc cggagaagat cgtccgggtg
     9361 aatgccgacc ctgcccttgg tttccattca taccaggcga aggaactggg cttcgccctc
     9421 ggtcttccgg ctgataaggt gaatggtttc gccaaaatcg ccgagcaact ctacaccgca
     9481 tttattaagg aggacgccac gttggtcgag gtcaatcccc tgattctcac cggcgccggc
     9541 gaatggcagg cgctcgactc caagatgagc ttcgatgaca acgcgctgta tcgtcacaag
     9601 gacatcgagg ggctccgcga tatggcggag gagaacgaga ccgagcttga cgctcgatcg
     9661 agcgggatct ccttcgtgaa gcttgatggc gatattggct gcattgtaaa cggcgccggg
     9721 ctggccatgg cgacaatgga tgccgtcaag ctgagtggcg gtgagccggc gaacttcctt
     9781 gacgtcgggg gcggagcaag tgcatcacag gttgcgaagg ccttcagcct cgtgacatcg
     9841 gatcccaacg tccgtgccat ccttatcaat atctttggcg gcattacccg aggggacgtt
     9901 gtcgccgacg gcatccgcga ggccctgggc caggtagagg tcaaggtgcc gatcgtggtg
     9961 cgcctctctg gcacgaacgc cgatgaaggc aagaagatcc tcgctgaggc cgggttgacg
    10021 gcagtcgaca cgatggacga ggcggctgaa caggttgtct ctgctgcatc aggacaggcc
    10081 gcgtaatcac gtcacccggg agcccgggga aggagaaccc agtgagtatt ctggtaggca
    10141 atgacacccg gctgctcgtc cagggcatta ccgggcggga agggtcgttc cacgcgacgc
    10201 agatggtcga gtacggcacg aacgtcgtgg cgggtgtcac cccgggcaag aagggtgagt
    10261 cggtcgccgg tgttccagtg ttcgatacgg ttgaggaagc ggtcagggaa accaacccga
    10321 acacctccat catctatgtc ccggctcgct tcgctcccga tgcgatctac gaagccgtag
    10381 actacggtat ccagctggtg atctgtatta ccgaaggcgt gccggtcaac gatatggtgc
    10441 cggtttatca ccacgtcaag cagaagggtg ctcggctgat cggtcccaac tgcccggggc
    10501 ttatcacgcc gggagaggcg aaggttggga tcatgcctgg cttcattcac gagcccggaa
    10561 aggtggggct ggtttcccgc tcaggaacct tgacctatga agtggtcgac gccctgacga
    10621 aggccaatct tggccagtcc actgccgtcg ggatcggtgg agatccggtg atcgggacct
    10681 cgttcgtgga tgtcctcgag cttttccaga atgatccgga cacggaagcg atcgtgatga
    10741 tcggcgagat cggcggtgat gacgaggaga ccgcggcagc gttcatcaag gagcacgtga
    10801 cgaagccgat ggccggtttc atcgccgggc gcacggcgcc tcccggcaag cgcatggggc
    10861 acgccggtgc gatcatctcc ggcggctccg gctctgccga ggagaaaatc gccgcgcttg
    10921 agtcggcggg ggtgcgggtc gcggacagcc cctcgaaggt gcccgggctg ctcaagcaag
    10981 ctatgaacgc gtaggtctat cgcctcaatg caacacgccc ggacgacgcg agaccgtccg
    11041 ggcgtgttgc cgtctggagg acggttcgat ggacggccct gagctgttcg acaacgggta
    11101 catgcagctc attctcggag atatcatacg gcagcgtgtg gatgcgatcg tgaatgccgc
    11161 gaattcctcg ctactcggtg ggggcggcgt cgacggggcg atccatcggg ccgccgggcc
    11221 aaaattgctg gagatatgcc gccggattgg cgggtgcccg accggggagg cgagaatcac
    11281 gccggggttc aatctgcccg cgcactacgt cgtccatacc gtcgggccga tctggcgcgg
    11341 cggaggtcat gacgaagacc ggctcctcgc cagttgctac cggaacagca tgcgcatcgc
    11401 ccatgaaagt ggcgtgcatt cgatcgcgtt tccatcgatc agcacgggca tctaccagtt
    11461 cccgctggag cgggcggccc ggattgctgt tggtgagttg caggaggccg cggagcggta
    11521 tccggagatc acggtgatcc gtgttgtcgc tttcagcaag gagacgtggg atgcctacga
    11581 gcgcgcaata gaagagtcga tcgactaacg atcattgccc gacacgccat cgctcatgat
    11641 ctcgtggacg acggggcttt cgaattcctc gcgcaacagg tcgtaataga tgtcatccca
    11701 atagcgtcca tcgaaataac gggcttggcg acggcgtcca aattccctga aaccagcctt
    11761 gacgtaggct ttctggcccg cgatattcca gccaaaggtg agcagccaga tgctgttcaa
    11821 ctccattgcg ttgaaaccat agtcgcagac aagccggacg gcctctgtgc ccaggccgcg
    11881 cccacgggcg tccctttccc caatcatgat gccgagctcg gctgtgcggt tcctgacatc
    11941 gatgccgtgg agatcgacgc caccgatggg ccggtatgac gaacgctcgt agatggtgaa
    12001 gaagaccatt cgctcattgt tgacaacccc ttcataccag ctgatctcct cgtccaggga
    12061 catcggcctg gacacgagga accgtgttac cgacaggtcg ttgatccacg cctggtagag
    12121 cggaaggaga tcgcgacgaa tcgggccgag tgcgattttg tcgccctcaa tggtgaccag
    12181 cggtcgagcg tcggttaccc gtctgtcact ttcagcgtga gtggaatcag gagtcacaag
    12241 cttgtctccg gcgtctcgat ccgttgtggc gtcttcttat ccggccggtt gggactcgtc
    12301 ctgtgcagcc gctcccaggt gccgggttcc ggctatcgcc agggccaggg catccttccg
    12361 caatgcgtcg acatcaacgt tgaccccacc ggacatcgcc atgctgatga cggcgacatt
    12421 tcccaccgga atggcgaccc actgcatggc aacctcgatg ccgttggtca ccgtggagat
    12481 cgttatcacg taggcgatcg tgtcgtcgac cgtgatctct gacacttcca cctcagctgc
    12541 ctggtctcca atcgtgctca ggagcacttc accaagacct tccagggact caggattgac
    12601 ctcatccccg taaaccaggt agttcaggga cgcagtgttg tagatgctgg ccggaccacc
    12661 cgctgcgggt gatgcgacgg gggaaggaga ggcggatgca cccgtgcctg gctggtagtc
    12721 gatcgagtac atgaccgtac cgacgacgtc tcccatcgag cccctggcga tattgcccgt
    12781 atcgccggaa acggacgccg gatcgacgta ttcggcgttg gagaatgccg cagggagacc
    12841 ctcttcgtct attggagcct tcaaggccgg gacaagcgac tctgcatccc gctctagaac
    12901 aacctcggcg atggagggaa tatcgatagc atcctgggcg ctgaccagcg gcgcaagggc
    12961 gagcatcgcg ccaaacagaa ggatgagtaa cgcgtgaaat gtgcgcatgg acgcattcct
    13021 ctcgagtagt ggtgtcgcca tgatcggctc ccttaaccgc acgggcgtga tgggacgacg
    13081 gtgctgtgtc cgccaatcat acccgcgggc ggtacggtgg caatgtcgcg attcaacgct
    13141 ccgagaagga agtccgaagt cgtcttgccc ttcgccgaca gaaaaccggt cccgacaaaa
    13201 gcgggaccga ttgtgcgtct acacataatt ttgttggtct cttggcgtct actgtgcgtc
    13261 ctcggcaacc gaaccaaggt agccagcacc agcgaggacc agatcctgtg aggccgcgag
    13321 cacggcgtcg gcgtccactt cggcccccgc ctggacggcc atggacatga ccatcgtgtt
    13381 tccgacggga atcgccacca actggacgac cgactggaca ccctcctcag tgatcaggta
    13441 ggtgatcggc gcgccctcaa ccccagctcc tccaccgtgg cttccattcc tggcgcgccg
    13501 gcttccggac tggcgtcagc acctgtctcg ccgacaaggc cctcctgggc gccggacttg
    13561 aattcctcca agtcatcggc tgtgatttca tcgtccatga agacgtagtt cagggaggcc
    13621 aaaccgatgc tgaatccagc ttctgccggg ctggatccgg gggtggcgcc cacactcctg
    13681 gctacagggt cgaaatcaat cgcgtaggct acgctaccct cggcgccttc gagatcgctc
    13741 gcggggagca cgccttcctc ggcgctggcg gtctcggggt cgacgaacgt cgcgttcgag
    13801 aagccctcag gcaggttctc gtcttttatc ggtgtctcaa gggccttcat tagtgcctct
    13861 tgatccgcgt ccaggaccgt ctctgcaacg ggctcaagat ccacgtcttc ctgcgccaca
    13921 gccagcggag ccgacattat ggcacaacat gctgccatgg caagcaccag aagaagcgtt
    13981 cacatatgtc cctgtttcct ttccccgttc ggttacgtgg caatccaagc cggcatatgt
    14041 cgatgccgga agcgagcgta accatagcac gcaggacggg cgttcacgtc cctgcagcaa
    14101 gtattgccgc tcaaggtgca tggtgccggt ggcggcgaag ggccttcgcc gccaccggat
    14161 tcaacagttc gtgagcgagt aaaatgcaag cacgaaatcc aagaccgggc agctggtgca
    14221 gcgcgaaggt tcccggcgaa cgaaaggatc ttgatgcatg cagacttcct cccggatctt
    14281 ttgtcctgat tgcggtgccc ggaacgaccc caccgcccat ttttgccaaa gttgcggtcg
    14341 atcccttgag gatgtccgag aggtagccgc ggccactaat ccggtgactg tgggcgatga
    14401 gaaggatcgg tccggtcggc tgctggcgga cgatggtttg cccatcggtg aggatttgga
    14461 cggcgtgcct ggcggcgagc gagtgctctg ggaagggcgt cccagcatcc tctggtcccc
    14521 gcgcatggcg ctcacgcagc gctataaact caccaaccag cgcctcatca tgacgtttgg
    14581 cttcatcggc cggcggaccg aggaaatcga tctcttccgg gtcaacgatg tcggcgtcaa
    14641 gcaacatccg ctggagcgca ttacccggat cggggatgta tacatttctt ccgccgatca
    14701 gagctctcct atcaggtacc tcaacaatgt aaagacgcct gaccgggtca aggatttgtt
    14761 acgcgagtct tcccgacaag aacgtcagcg gcggcgcgtg ctcctgcgcg aggacgtcta
    14821 gcgctgggcg gaatcgacat ccagggcagt gtgtcggccg acggcgcgtt ctcgctcctt
    14881 gcaccagccg aaaccaggac aaggatggtc ggacgaggca ccctcttttt ggccggttga
    14941 acggcagtga ttgttcagga ggagctgaga atgttctcga tcgatgcgct ggccgtgccc
    15001 atcatggcag ccccgatggc cggtgggcca tcgactccag aactcgttgt cgcggctgct
    15061 gaggtgggcg gcacgggctt cctcgctgct ggctacaaga ccgctgccgg gattggcgaa
    15121 gagatcggac gcgtgcgagc cgcgacattg gcagccttcg gtgtcaacct ttttgtcccc
    15181 tccgggttgg ccgctcaaga tgttgaccgt gcgagggacg tggaggcgta ccgccgagca
    15241 ctgcagtccg aggccgaacg ataccaatgc acaattccat agccggacgc cgccgaccgc
    15301 gacgggtggg aggagaaaat tgctcttttg ctcaaggatc cggtccccat cgtgtccttc
    15361 acattcggct gccctgaccg catcacaatc gagctgttgc aacgcgcggg aagctatgtc
    15421 gttgtctcgg tcactgatgc agctgaggca gtgctctcgg ccgaccatgg cgctgatgcc
    15481 ctctgcgtcc aagggccgca ggcaggcggg cacagggcca cgcatcatgc cgcgcaagag
    15541 ccaggagcaa caggattgga gatcctcctg aggaacgttc gcgatgtttg cgatctacct
    15601 ttgatcgcag ccggcggtat caccagatgg gaacacatag ttgccgcaat ggaggcaggg
    15661 gcgaaggcgg tccaggtcgg cacggttctg ctgcgatcgc cggaaaccag ggcgtcgacg
    15721 ctgcataagg atgcgcttgc atctgaccgg ttcagcaaca cgatggtgac gcgcgcgttc
    15781 tccggaaggc ctgcgagagg gcttgtgaac cggttcatgc gtgactatca cgacgttgcc
    15841 ccggcggcgt accccgaggt gagtcagttg acccgtccac tgcgggccgc tgccgccgcc
    15901 gctggcgatc cggacggcat ggcgctctgg gcgggcacgg gttttcgaga tgctcccgtt
    15961 gctccggcgg ctcaaatcat gacctccctt tgggaggaag ccccagctga gcgttcatgt
    16021 tgagcgattc ttcccgaacc gacgggagaa tcacatattg cgcgtacact tgaatcagag
    16081 atatctgaca attcaagatg gctgaacagc ccgctccagg ctaggccgcc ggcgggagca
    16141 tacccgatta gtgcagtgat ctcgtattcc agtctggggg cgccacacag tggaagacat
    16201 tggcctcatt attgacctgg tgacgacgct gggtatcgcg ttggtcggcg ggctgatcgc
    16261 gcgtcgtctg ggtatgcccg tgctggttgg ttacatcctg gccgggattg ttatcggacc
    16321 gcacacgccg ggactggtag cggacgggga acgcgtcgag ctgatggcca acctcggtgt
    16381 ggccttcctg atgttcgcac tcggtgtgga gttttcgctc gcccagttgc tctcggttcg
    16441 caagatcgcg ctcaccaccg gcgcaatcca gatgcccgcc tccgtcctcc tgggcactgg
    16501 agctggcatt gctctcggtt ggggtatcaa atccgcgatt ctcctcggcc ttgcctttct
    16561 cgcatgcagc tcaatcgtgg tcatcaagct cacgctgagc cgtggcgagg cgaccagccc
    16621 ccatgcacgg gctgccctgg gtcttgggat tctccaggat ctaagtatgg ttccgctgtt
    16681 ggcgctcttg cccatgctga agccggatac cactgaggtg atccttcctc tggcgaaatc
    16741 gctcctgatg gctgcagctg ccctggtcat tgtgcttgtc gtcggcacgc ggctggtgcc
    16801 taaagtcttc aacttcatag cgaaatccgg gacgcgggaa ctctttctcc tcaccatcat
    16861 ggtgatcgcc ctgggtacgg cctacgcgtc tcacgaagcc ggtctctcgt atgcgctcgg
    16921 ggcctttctc gcaggtctgg tcgtctctga atcagaattc gatgcccatg tcctcgcgga
    16981 aatcattccc ctgcgggatg tcttctcgac gctgttcttc gtatcactcg ggatgttgct
    17041 ggacccacag ctgttcctcg accatcccct gatgatcgcg gtggtcgtgg tggtactcgt
    17101 caccggcaaa tttctcgcat cgctggcggg ataccttctg agcggggtcg gaccagtggt
    17161 ggcaacgaag gcggcgctgc tgaccgccca gatcggcgag ttcagttttg tgctggcaag
    17221 tatcggacta tcggatgcca tcatagacga tgaccagtac agcctgattc tcggtatcgc
    17281 gctggcctcg atcctccttt cgcccttgtt gctgatggct gatcccttta tttctcccgt
    17341 catccagcga ctcccgtacc tgcgggacca catgctttca tcggtcgacc gggaggcgtc
    17401 atatggcgaa atgcaggacc atgtgattat ctgcggttgc ggaagagtcg gggaggccct
    17461 cgccgagacc ctgatccgcc ggcagctgca atttgtcgtg atcgacatga atcctgcggt
    17521 ggtccaggat ctccaatccc gaggaatacc ggccctctat ggggacagcg ctgcagagcc
    17581 ggtgctgcac cgtgccggcg tcgaacatgc caggatcgtc gccgtgacag taccgaacac
    17641 gattgtcgcg ctgagaactt cgcgcattgc gaaggaattg aatccggacc ttgagattgt
    17701 ggtgagggct ggacgggcga tggagatgca ggggcttcgg gtcgcggggg cgagtgacgt
    17761 ggtgcagccg gagttcgagg ctggtctcga gttcatgcag catgtgcttc ggcatcaggg
    17821 cgtttccgcg atggagaccc gggagatcgt ggaccgacgg cggcaccggt tctatcagga
    17881 cgatgcccgg gcactcgctt ttcacgacga tgcgtgacat cgacttgcag cggtacttca
    17941 tgcaacaaag gaacgtaccc atgactcacg acatccatat caacaccaca ccgggagagc
    18001 cggatgtcga tttgaccggc aagaagctgc ctgtaaccgg tggatcccga ggagttggtg
    18061 ctgaggtcgc gcggattggc gctgtctgtg gtgccgatgt cgccatcaac taccggagca
    18121 agcttcgtcg cgccgagcaa gtcgcgggga aggttcggga gtagtgacgg gaagcgctcg
    18181 ttgtcgaggg cgatatcacg gatcccggtt cagtccaggc gatgtatgag cacgttggga
    18241 acgtgtttgg aaagctggat gtgctcgtcc tcaacgcgag tggagggctt gagaaggatg
    18301 tggatgaggg gtacgcgatg acgctcaatc gtgattccca ggtggcattg gccaaagggg
    18361 cgctgccgct gatgggcgag gggagcgtta tcgtcttcgt gacaagccac ctcgcgcact
    18421 tccatggcga gaaggacgtg atgccggagt atgagccggt cgccgcgagc aaaaaggcag
    18481 gcgaggttga gctgcgggcg atggtgccgg agcttgagca ggcaggagtt cgccttgctg
    18541 tcgtcagcgg taacctcatc gatgggacga tcacgccgaa acttctggat cggatgtgcc
    18601 cggggattat tacccagcga cgggaagagg cagggtggct cccgacgact gaggacttcg
    18661 cgtcgtcgat cgttttggcc acgagccggg atgacctgat ttcgggcgcg accatctacg
    18721 tggggagcac ggagtggtga acaccggttc ttcaactacg ctcggcgcta tgccttgttg
    18781 cctgcaatcg gttcgcgctc ggaagcttgt gagggttccg gacttcggac tgggtccggg
    18841 agcagacctg cggccaactc aaccaccgta ttaagggcca tctctcggcc agttgtccag
    18901 gccacatggt acgcatccgg gttcgagaag gcgtggcggg cagcgtccat atggcggcgg
    18961 taggctggga gctccgcagg cttgaggggc acaccgacct gctcccgcaa tacctcggcg
    19021 gccccgccca tctgtgccgc cacttccggt cgctcgctga cagacgccgg gccggccagt
    19081 gcctcgaggc accatgccat gcccaaccgg tcaccgatct gttgatagag gaggaggcct
    19141 tcgacgagcg gctcggtagc ttggggattg tcttcgtgat gctgtagcag cgagcccagg
    19201 ataaagagcg ccgttgcggc cgaacgcttg tcacccacct cacgggagat ctggaggccc
    19261 tctcgggcga cggtcaccgc gtgctcgagg tcgccttccg cccgcagcgc ctcgcccagg
    19321 ttggccagtg ccacggccgt gtgcggacgg tcacccagtg ctcgccagtg gatcagcgag
    19381 tcttcgaacg acatcgctgc ccgccgatag tcctgctgcc agaaggccag aatgccgagg
    19441 ttgttgagga cgcgagcgga cccatgccgg tcgccgatga gatcgtaaag cgatccgctt
    19501 cgctcgtata ggcccctcgc tcgcgtgtac tcgccctggt ctgtcgctat gatgcccaat
    19561 tggttgagga cgcccgcgat acccgcgcgg tcgttaatcg tctcgaagag cctcagtgct
    19621 tgatggttga gttcgagtgc taccacatac tcgccacggg cagtcgcgat gccggccaag
    19681 ggaacgaggg tttgtgcggt ccggcgcgag tccccgagca cccgccagtc gccgagtgct
    19741 tctgttaacc gggcgattgc ttcgtcattg tcgccttggg cctgagctag gacgccggcc
    19801 cccctcaagc acaacgcgcg ccctgcggac ggctgactat ccgtcagtgc aagagcgcgt
    19861 gccaaccaca ttcgcccctc ggtgtggtgt cctcccatcc gccagaactc cgcgagcgcg
    19921 ccactgatgc ccagggccgt ctccgcctcc tgttgctcgc atgcccaatc cagggctgcg
    19981 cgcaggttgt cgtgctcgac ctctagttgg tcacgccaag agatctgatg catctcgtgc
    20041 aagaggggct cggcattctc ggccgtagcc aggaagaagg cggcgtgcct tcgtcggatc
    20101 gtctcttcct ccccactagc cttgagtttc tccaatccga attcccggat cgtctcaagc
    20161 atccgatagc gaggcgaccc tccctctgtt ccctcctccc gctgcaggag gctcttgtcg
    20221 gccagtgccc acacgttcga ggcgacctcg ggaatgacat cgcccaatgc gtggcagacg
    20281 gcctcggccg catcgaggtt gaagccgccg acgaagaccg cgagccgcgg aaacagcgtc
    20341 tgctccgctg gttccagcag gtcatggctc caggcaatgg catcccgcaa ggtgcgctgg
    20401 cgttccggca gatcccgcgc cccgcccgtg agcaggggca ggcgtcgcgc cagtcgcgcg
    20461 agcaaatcgt cgggagcgag gtggctgctc agggcagcgg ccaattcgat agccaacggc
    20521 atcccgtcca gccgggtgca gagcgcggcc accgccagag cattggcatc ggtgactgcg
    20581 aaatctgacc gggcggcgcg agagcgctcg gcgaacaggg ctacggcctc gtaccggctg
    20641 acggaacttg ccagaagagg agcagggaag gtgcgcagcc tcaggggagg tacggcaagc
    20701 ggggggattg taacgacatg ctcggctcgc acgcgcaacg gctcgcggct ggtggcaagg
    20761 atcgtcaggt gtgggcatcc ctcgagcagc ctggcgatcg cctctgccac atcaatgacc
    20821 tgctccagat tgtccagaat cagcagcagc tcgcgttcgc gcagggcttt gccgagcaac
    20881 tgggccactg gatgcccggt gacctctttg atctccaacg tgtgggcgat catgggaagc
    20941 acgacctcgg gatcatggat cgctgccagt ggcacgaagc agaccccatc cgcgaagtcg
    21001 tcgctgattt gccgcgctac cgccagagca agacgggttt tgccaatgcc tccggctcca
    21061 gtgacggtga caagttggac atccgggtgc aggaggatgt cctgcaagga ttccatctcg
    21121 cgaccccggc cgattagtgg ggtaggtgtt gcggacaggt tatccccccg gcccgcggga
    21181 gcggccgggg gcatcggcag tgacggaacc tggtaagccg accggatctc ggtggccagc
    21241 gccaccgtct caacgtccgg ctcgacatcc agttcgtccc gtagttcctc gacgcagcgc
    21301 gcgtactggc gaagagcctc gtcgcggcgg cccgctgtcg cgagggcgcg catgagggcc
    21361 cggtgcgccg cttcatcggt gggatccgcg ttcagcaact tctcaagtac gggaaccgac
    21421 tggagcgggc agcgttccag ttcaagttca gcgagctcca gcaccgcccg gcgccaggtg
    21481 cgccgtagcc gctcgcgagg ttccgccgac cagtcagtgt acggctcatc ctcaagcaga
    21541 tcgccgccga agagtgccag agcctcatgc agcatcaccg gacgatcggc gagcgcagct
    21601 gtccccgccc ggatcgccgt tgcctcgaag acttcgaggt cgatcgagag cgcgattccg
    21661 gaccgcaggg ccacagcatc acccctgctc tccacatagg cggattcgcc cccggctcgc
    21721 aggtccggtt cgaggacgcg gcgcagggta tggagcgcca cgcggagagc cccgtgcgcc
    21781 gactccggcg cgcgttcggg ccagaggagg tcaaggacgc ggtctctcgg gaggcgatga
    21841 cctggggtga tgagcaacaa tagcaggagc gcccgagcgc tgcgtcgggg ccaagcgtga
    21901 tccgggatta cccgattgcc aacagcgatg cggactgggc ccagcaggtg tgcgcacagc
    21961 gggaccaggt aaggggcggg aggcaagtcc atcggaacct ctcgtcggat ttccatttag
    22021 ttgagacgta gcgggcgggt cacaggaact gcgttcatgc tagggggcca gtccatcggc
    22081 gtcaagcgcg gtgaaagggt ggccggcctg gggcccatgg gaaatgatac aaaggttggt
    22141 gggggcgaat tcgggtggta agcgagaaga cacgatgtct ttgcgccgat gggcccgcat
    22201 ctactgttac cagaatgtcg atactagatc catgccgggg ttacagttgg tcccggataa
    22261 cctctgcggc aatggggcac tcgtaacagc ggccgggcgt gcagagagtg cggtcaagat
    22321 gcaggagtcc ctggtggccg cgctcgccca agtcacgtag cgatgggccg ccggtgacct
    22381 gccgcagggc gcgcttggcg ggacgcgtcc attccgcatg cgtgagtgac acccacgctt
    22441 gggatgccgc atcctccaga ccagcgtcct cagaatgggc tgcccaagca agggcgaaa
//
LOCUS       DASYWM010000115         4017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_154322, whole genome shotgun sequence.
ACCESSION   DASYWM010000115 DASYWM010000000
VERSION     DASYWM010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154322"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VGR29_04765"
     CDS             complement(<1..823)
                     /locus_tag="VGR29_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072936.1"
                     /translation="MKSRGRAIPNVAMIAFGLISLLCATVRPVSASTLDLAPVQDVTQ
                     TVEQTLEPANELTAPVLEPVEEQPLVVEQPALEPVAKTVAEPVIQAAEPVADYVEPAY
                     VEPAAVVEPVYTEPVTNTVSESVAEVTEPVADVVEQPALEPVAEAVTEPIFVPEESAG
                     AVTEPVADITDPVTNVVEAVVEQPALEPVADTLSEPVPVEEPVAAVNETIAGATQPVE
                     VVEPITEAVVAEAVQASEVVEPVAEAPVTEPVASVMQPVTDVVAGTDIIDGLGAIE"
     gene            1485..2603
                     /locus_tag="VGR29_04770"
     CDS             1485..2603
                     /locus_tag="VGR29_04770"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV2072937.1"
                     /translation="MNSTEAVIRLVLLGMMLLLLVGGAVAMLLLWYLRGRDPHTGLVA
                     DILSEPPDDLSPGAVGTLLDEHADHEDVVATLLGLGRNGAVVIEAASTPNPGARDYRI
                     TLLHPERITRPLERDLLRVLFGSDPQPMMEVQLDQVRSRFVDAEPKIRDDLYEELVQR
                     HYFAMNPHRTRERWRRISRAGLVLSLVVGLILSVLVDPFAIATTIASAIIWTAMIRMS
                     RHMPRKTPEGTEAAAKWRAFKRYLQSIEPQRDLKEASDIFDRYLSYAVAFKMDRQWIS
                     MFSAAGVAKPGWFGGPDIGDIVILGDMGNLGDVAGAGRLFGDLGNPVPDISMPNVSGT
                     SMQGAADALGGSLQGASDGLTGLLDAAGSIFDDIDFDL"
     gene            2581..3384
                     /locus_tag="VGR29_04775"
     CDS             2581..3384
                     /locus_tag="VGR29_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV2072938.1"
                     /translation="MTSTSISDLRPRETILGVPVDIVSRADTSAWIRNRLSAGKSCAH
                     IVTLNPEYVMTCRRDAAFHTIVQEADLVTIDGAGVAIAVRWLTQIRSVERVTGVEICW
                     IAASISAESGAGIFLLGAGPGVADRSAEKMLKVSPGAVIAGTWAEGSPHQRDDAETIR
                     RITASGANVVLVAYGAPGQVHWIARNQSALSGAGVKVVVGIGGALDYISGNVSLAPAL
                     VRKLGLEWAYRLAREPWRWRRQLVLPQFALLVLRDALLQRVGRATSHVE"
     gene            3598..3981
                     /locus_tag="VGR29_04780"
     CDS             3598..3981
                     /locus_tag="VGR29_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072939.1"
                     /translation="MPSIRTPGSDLLKAIGGGLLVALVVAVIWRFIPEWGFYLSLAMG
                     FGVVETIAKLTRNKRGADLQLLAMALITIGFLVSRVLLANRYDITFGEINQLGEYAQR
                     ALRVRLVPDLVYMALAYAIAWIRFR"
BASE COUNT          764 a         1132 c         1249 g          872 t
ORIGIN      
        1 attcaatagc gcccagtccg tcgatgatgt cggtgccggc caccacgtca gtaaccggct
       61 gcataacgct ggcaacaggc tccgtgacag gtgcttcagc aaccggctcg acaacttccg
      121 atgcctggac cgcctctgcg accaccgctt cggttatcgg ctctacgacc tctactggtt
      181 gcgtggcacc ggcgatcgtc tcgttgaccg ctgctactgg ctcctcgacg ggcaccggtt
      241 cggaaagcgt gtccgccacc ggctcaaggg caggttgctc gacgaccgct tcaacgacat
      301 tcgtcacggg atcggtaatg tctgctactg gctccgtaac agcgccggcc gactcctcgg
      361 gaacgaaaat cggctcagtg acggcttctg caactggttc gagcgccggt tgttcgacga
      421 cgtcggccac cggctccgta acttcagcga cggactctga cacggtattg gtgacgggct
      481 cggtataaac cggttcaaca acagcagctg gttcgacgta agctggttcc acgtagtcgg
      541 cgaccggctc agcagcctgg atcaccggct ctgcaactgt cttagccacc ggctcaaggg
      601 caggttgctc gacgacaagg ggctgctcct cgacgggttc aagaaccggc gccgtgagct
      661 cattggcagg ctcaagagtt tgttcgactg tttgggtaac gtcctggaca ggcgcgaggt
      721 caagagttga ggcggaaacg gggcggacgg tggcgcacag caacgagatc agcccgaagg
      781 cgatcatcgc aacattggga attgcccgtc ccctagattt catcaatcca tcccatggtg
      841 ctgcggactg cgttgccgtg tccgcagtgg ataacgatag ggccttgagt tgttcaatgc
      901 gtttccctgg cctttacgca acagagaaca catatagggc ggacgagtga atcgttgccc
      961 atacaagaat ctaccgtttc cccctgcaaa caggttacat cgccataata aataacgcaa
     1021 ctatacgaag gcaccaaatc gcgtatacga aagtgcccac cctgctccag gaactggatg
     1081 gtgtgagggt ccctagccgc ctctatcttc gcccatccga gggtcattct gctacggctg
     1141 cgggacagtg tctcgctctg gcggcatatc ctatggcaat cccgttccgt ttgctggaag
     1201 gtggaggtgt cctccaggcg aggacgagct ggttcgagca atcgcatctg aagcgcagag
     1261 caaacgcgcc ttccttacag cgcttatgtt cgcgcagata aagcaagtgg aatgcaacgg
     1321 gatgcaaaaa ttattcgtct taccgcagtg aagggtggcc aattacggga atagggatca
     1381 cgaggacgag gacggccacc atctgccaca gtgggatcta cgccattgac gcgctcccga
     1441 tgagcgatcg aagctctaga taacggacag ggcacttcga cgaaatgaac tcaacagagg
     1501 ccgttatccg actcgtactc cttgggatga tgcttctgct gcttgtcggc ggggcagtgg
     1561 caatgctgct tctctggtat ctgcgaggca gggatccaca tacaggactg gttgctgaca
     1621 tccttagtga gccgccggac gatctgtctc ccggtgcggt ggggacgttg ctcgacgagc
     1681 acgccgatca cgaggatgtt gtggcgacgc tgctcgggct gggccgaaat ggcgccgtcg
     1741 tcattgaggc ggcttcgaca ccgaatcccg gagcacgaga ttaccggatc acactgctgc
     1801 atcccgagcg catcacccgc ccgcttgagc gggacttgct tcgcgtcctc tttggttccg
     1861 atccccagcc aatgatggag gtgcagcttg accaggttcg ctcccggttt gtcgatgcag
     1921 agccgaagat tcgcgatgat ctctatgaag aactggtcca gcgtcattat ttcgccatga
     1981 atccgcaccg gacccgtgag cgctggcgac gtatctcacg ggccgggctg gtgctctcgc
     2041 tggtcgttgg gttgatcttg agtgtccttg ttgatccctt cgcgattgca acgactattg
     2101 cctctgcgat catctggact gcgatgatcc gaatgagccg gcacatgccc agaaagacgc
     2161 ccgagggcac ggaggctgcg gcaaagtggc gggccttcaa gcgctacctg cagtcgattg
     2221 agccacagcg agacttgaag gaggcctctg acatcttcga tcggtatctc tcatacgcgg
     2281 tggcgtttaa gatggatcga cagtggatca gcatgttctc ggccgcgggt gtcgcgaagc
     2341 ccgggtggtt cggtggacct gatattggtg atatcgtgat ccttggtgac atgggaaatc
     2401 ttggagacgt tgcgggagcc ggtcgattgt ttggcgatct cggtaatccg gttccggata
     2461 tatccatgcc gaatgtctcg ggcacgagta tgcagggtgc cgccgatgcg cttggaggct
     2521 cactccaggg tgccagcgac ggattgacag gactgctcga cgcggcgggg tctatctttg
     2581 atgacatcga cttcgatctc tgatcttcgg cccagggaga cgatcctcgg ggtaccggtt
     2641 gacatcgtct cacgggcgga taccagcgcc tggattcgga atcggctttc ggccgggaag
     2701 tcgtgcgcac acattgtgac actgaatccc gaatatgtga tgacgtgccg cagggatgcg
     2761 gcctttcata cgatcgtgca ggaagccgat ctcgtcacga ttgacggggc aggagtggcg
     2821 attgccgtcc gctggctcac gcagatacgc agtgtagagc gcgtcactgg tgtggagatc
     2881 tgctggattg cggcgtcgat atccgctgaa agtggtgcgg ggatcttcct gctgggggct
     2941 ggacccggtg tcgccgatag gtccgcggag aagatgctga aggtgtcgcc cggtgcagtg
     3001 atcgccggca cgtgggcgga agggtcacca catcagcgtg atgatgcaga aaccatcagg
     3061 cggatcaccg cgtccggcgc caatgttgtg ctggtggcct atggtgctcc tggccaggtg
     3121 cactggatcg cccgtaacca gtcggctctt tccggtgcgg gtgtgaaggt ggttgtcggg
     3181 ataggtggtg cgctcgacta catttccgga aacgtctccc tggcacccgc cctcgtgcgg
     3241 aagctgggcc tcgagtgggc ctatcgcctg gcgcgggagc cctggcgttg gcggcgacaa
     3301 ctcgtcttgc cacagtttgc actgctggtc ctgcgtgatg cgctcctgca gcgagtaggc
     3361 agggcgacat ctcacgtaga atgaagcgcg ttcggattga ctgatcctga aacgattcaa
     3421 acacgaaaga gttcaaccgg ttccatgcaa atcccccgtg gagaggttcc ctgtacctat
     3481 catcccaatg tccaaactgg attgcgttgc acgcgttgcg gcaaacctat ctgtccccag
     3541 tgtgccgtcc gttcacctgt cgggctgcgc tgtccggatt gcgccggcgt acggagcatg
     3601 ccgtcgatca ggacaccggg gagtgatctg ctcaaggcca tcggtggagg gttgcttgtc
     3661 gcattggtgg ttgccgtcat ctggcggttc atcccggaat ggggattcta cctctcgctg
     3721 gcgatgggat ttggcgttgt ggagacgatt gccaagttga cccgaaacaa gcggggcgcc
     3781 gatctgcaat tgctcgcgat ggcactgatc acgatcgggt tcctcgtcag ccgtgtgctg
     3841 ctcgccaacc ggtacgacat caccttcggg gagattaacc aactgggcga atatgcccag
     3901 cgtgccctgc gcgtgcgcct ggtgccggat ctcgtctata tggcactggc ctatgcgatt
     3961 gcctggatcc gcttccgttg agggcgaacg ctcctgatgc ctctgttgcg cctcccg
//
LOCUS       DASYWM010000116        11441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_342020, whole genome shotgun sequence.
ACCESSION   DASYWM010000116 DASYWM010000000
VERSION     DASYWM010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11441
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342020"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGR29_04785"
     CDS             <1..220
                     /locus_tag="VGR29_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072940.1"
                     /translation="DFLKEYNPQLIHLLPSFEEIVRRNGTRPPRLTDEELKMVYEGQA
                     QLRVFDTQIDNTHLSPDEAADAVLQLMA"
     gene            complement(791..7138)
                     /locus_tag="VGR29_04790"
     CDS             complement(791..7138)
                     /locus_tag="VGR29_04790"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HEV2072941.1"
                     /translation="MSETRSRSAMEAVSGRARFGTARRRSTRRAITMRGVGRFLHLFG
                     AIAMLIGLFLPLTDAGRALAAPGAQNNLAQTQVQDLSTASFTEVAAIGDFQQQFGCNP
                     FDINCPGTQLTNHEGIWTGVFPIPPGQWQWQIAAMTQDGQQVFLSESGPDQTDTVTIA
                     ETDAGVYFRFNALRQEAEAVPVASIATLATDAGTYALRPDDGTLTAIVQALQPGPVNA
                     ELQIDGVPVGNPQVITAPQGPVRVTLDASGTVIEAEDLGYGTLTITRVDGGTGQPLPG
                     GCYEVREGGTVINRGCDTDDGVADGSILLRFPEGLEPGSYTVAEVRAPEGAEQAPDQE
                     VDLQETDNSIQISTGEPEDEPTAEDGGILPIGEATEAPQGDDVAVTPTATTEAPAAAV
                     PGNLIVTLQDQNNTPIGGACFQLVQGGEVKAEACDLNDAFPNNGNTGFFGVPSGTYTL
                     HQSTAPEGVQPSPDREVQVPAGGEETVVVQAAAPAEPTPAPTGNVVVLRQDAQGNPVG
                     GACFELLDSNGGVVGEAVCDEDGDVADDGRIGFFDVPAGSYTLHESRTPAGYNPAADA
                     PVQVTGNEVSEVRVQGAAIPTAVPTDVPTEIPTPTPTNVPTEVPTVAPTAEPTSEATP
                     TEAPAEDAVPGDLIVTLQNEAGQPIGGACFELVRGDETIGPVCDSDDPFPGNGNTGFF
                     GVPSGTYTLHQTTVPDGTQPVADQEIEVPAGDRNTVIVGAPTTAQPTATPAPTVEPPG
                     APQDVAGATGGDVVVDIAAVDQSQGPVCVELNTSGGIGLLEAPNACNNGEGDAAPAPE
                     EILIEDVPEGNYSIFVREGPDELVEAEPAQVSVVEGETARVAIAAEAPEPTATATPEP
                     GVLPIVAVNAAGEPLPGACWTLTVGDAPLGPLCDDGTNGGEAGDGQVQFEGLVPGTFT
                     LTQTQAPEGYQPVEPIPVDIAAGENAALQVPHDVVVSSIDITTTNAAGESLAGACYAL
                     DGGEPICDDGDGTVTITGVRVGNHTIAQTTAPEGYRLAAEQSVTVTAAAPASVTFQNV
                     ALTGSVSIEVVDPDGNSVPGVCYTIDGGAQQCDDDGTDANATPGTIQVDGLALGEHTV
                     ALQSVPEEFQVPAEGMTVTVEADTVTPVRFELARVAPTTGGLDVTVQLESGELVPGVC
                     ITLTSTADASVTGPFCDNVEGDTNDQAGIIGIDGVGVGAWNVSLAEDTEVPGGDVGGV
                     EPRSVEIIAGERSSAILIVPLLPQNGTVRIVTTDGTTNLAGACYELSGPGGVMSVCDN
                     DETTDNDGTEGVIELLDVAPGAWTATMTTAPSGYEVAVPVDLTVVAGETASVEVAVEA
                     VPQPGSLTVTKLDGNGQALGGSCFAVQQGDATILSICDDTDANPNDGVLVFTDLAAGS
                     YQLVETRTPSNQYQRAEAQTVEIVAGEDTAVEVRNLPRAGRLAVITVQEADPAQRLNN
                     ACYRLEGATIYGPFCDADDGTVDARTSFVNVTAGDYTLAQTVAPIGYDVAASRPVTIG
                     AGSSLQVTVANAATPPAAEIGSLVVIPLDEHGAEVSGGCYQVLSGDTPLTGRVCDNDD
                     DVDKRIVFPELPVGTYTVRELLAPSPDFEIAPDQEVTITLNEQTEVQFAHTLKAGRVL
                     VQAVNTLGQPLQGACFDLANDGQDPACSAGSGEVLFEGVPAGTESLTQTQAPYGYKLN
                     DQAREVVVSPGQTTVVRVVFETAPPPDTGSVQVQKFICPAGEEGERTQFLGGAQGNAQ
                     LAKTAGCTQGEAAFTLVAEDGSGNGPGEFSTGEDGRYQVTLKQGLYVLTEINPDLPGS
                     SAARLRVGVGQMTTVIVINYIAPPEPVPASIEVTSYTCPPSFNGTAYGDFAESCTSDQ
                     ALTNTLTVRVEGAQNYKRVTGDTGELGRTRFADLPAGKYEVYGEKPYNVPIMYLFCGT
                     NADAPADVKTINGSAPVTLANGQTVTCHFFLVPEHLTKDTGAILVQKFSCPVDKPAKG
                     YDWANECDRSMEQVPFSLDVFNREMQEFEPLTEVTANPDGLVRFPNLRLGTYKLEEVG
                     SRWCFAQSNSVNAEGNVVVQPNKLAEVWIYNCVGTNQPPNTGSGDAASLLNPVDDPNT
                     GTMVLLNFAWPAIALAAWLGWRARHQRPSPVIVRRGENRAA"
     gene            complement(7951..9066)
                     /locus_tag="VGR29_04795"
     CDS             complement(7951..9066)
                     /locus_tag="VGR29_04795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2072942.1"
                     /translation="MAEAAAPLAYCLDNSPAPWARAQGQGNRAAIARAVNWTIELGRI
                     ADRAGIESLWLLEDPDGWDAATVLGAIARQTEKIRLGTGVMNPYYRHPSLIAASMSTL
                     DLLSDGRAFLGLGRGQSEWYEIAMGLEVGKPTRRLAETFDLLRQWWSPELVANSPEEA
                     TEFAIRDWERVFRPLQSRFPIYLAAVGPIAMRLAARHADGVIFNDLASMQFMREAIAT
                     VRGEAERLGRDLTGFLFAARSQVMVTDDPEKVYERRKSTVAMIHTLPQMERLMVTEGY
                     DVEQIIRDVRDVMRTQEILSQGGGFNALRRGGDLQAAKKLIPTSLMEELVVAGSLANV
                     RARLKQMHEAGVTHVFLATPPIGTTADELEDLVASLR"
     gene            complement(9072..9329)
                     /locus_tag="VGR29_04800"
     CDS             complement(9072..9329)
                     /locus_tag="VGR29_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072943.1"
                     /translation="MEPQEVWSSLENIFNMEAYVRYTSADVERLHRRCVREVEKMSGT
                     EVRLSLSRYVRDTMLSEEALADDKGWEEVLSFLDWVDGGME"
     gene            9765..10340
                     /locus_tag="VGR29_04805"
     CDS             9765..10340
                     /locus_tag="VGR29_04805"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEV2072944.1"
                     /translation="MNGPLDTAQWLLGLDADSLSVWQMAIRALIVYAAAVMMVRVGEK
                     RFLGKNTAFDVILGIILGSVVSRAITGSSPFFPTLGAGFVLVGLHWSIAVLAFRSERL
                     ATLVKGESRLLIRDGKVQQEAIHKSHIGERDLLSALRVNGQLTSVSDVAEAHLERSGD
                     ISVIERETEREPVILDVAVEDGVQTVRIKLG"
     gene            complement(10388..10726)
                     /locus_tag="VGR29_04810"
     CDS             complement(10388..10726)
                     /locus_tag="VGR29_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072945.1"
                     /translation="MSRKQLGSKTHETRGAGYETTFESGWVLTVFPNEDSTMTARLGC
                     RSHAFEVYSPEATMLGLPSFGFAIRVSFDQFGVPNVQWFHEANGDIFAWFCALGHAGW
                     STPVEGKRYA"
     gene            complement(10849..>11441)
                     /locus_tag="VGR29_04815"
     CDS             complement(10849..>11441)
                     /locus_tag="VGR29_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEV2072946.1"
                     /translation="ISLLIRAGNAGAITVVASLILSFTGSEDGAPERFLVLVAGLVGL
                     VALTRSRWFNRMLTPVIQRALSRSTTLVLRDYAALLHLREDYRVAEVEVRSGSWLKGQ
                     QLCTLDLPAEGLLILGIVQPSGGYVGAPPADYVFSEGDTVVIYGREQHLEEIARRSSS
                     DDLAHEEAKADHTAEVDAKTSIEPGSGAHRSSDPDA"
BASE COUNT         2081 a         3696 c         3456 g         2208 t
ORIGIN      
        1 ggacttcctg aaggaataca accctcagct tatccatttg ttgccttcct tcgaggagat
       61 agtccgtcgc aacgggacac gccccccacg actgaccgac gaagagctca aaatggtgta
      121 cgaaggtcag gcgcagctcc gcgtatttga cacccagatc gataacaccc atctgtctcc
      181 cgacgaggcg gctgatgcgg tattgcaatt gatggcctga tcatcggttc caacaccgtc
      241 ctccagccat tgacctgacg cggcttgcag gaccattgac cgttatgcct gtagcggacg
      301 acgaataggt agccacgtcg tgactgagcg agagcgaccc cttcggccga gcccggtggg
      361 tgagaagcgt cagagagcct ttgggtgagt tcctgggcga aaagcggtag cggacgacgt
      421 ataggcagtc gcgtagtgac tgagcgggag cggccccatg gctgagcctg caaggcgaaa
      481 agcggcagcg agcccttgcc gcatcaatgt cgtccttcca cgggtgagga cattgctgca
      541 tgtggtccgg cgagagtgta ggagaaccgc aagttgcatg gctgagccct tcgctacaga
      601 cagatcatcc tgaaaacttt cggcaacctc tctgcaaacc gctctggcat gccgatgaag
      661 gtcgcccaca cgggggcctg ctcggcaccc ctgcccaaac cccagcgtag acaccaggac
      721 cccgggtgag agcgtacgct ccggcccggg attctggtgt tctggtgtgc tgtgcaattg
      781 acctgtcgac ctatgcggcg cggttctcgc ctcggcgaac gatcaccggc gatggccgct
      841 ggtggcgggc tcgccaaccg agccatgccg ccagcgcgat tgccggccac gcgaagttga
      901 gcagcaccat cgtgccggtg ttcggatcat ccaccggatt gagcagcgac gctgcgtcgc
      961 cggatccggt gttcggcggc tggttggtgc cgacgcagtt atagatccag acctcggcca
     1021 gtttgttcgg ctggaccacg acgttcccct ctgcattcac gctgttcgat tgcgcgaagc
     1081 accacctgga gccgacttcc tccagcttgt aggtacccag tcgcaggttc gggaaccgga
     1141 cgaggccgtc cgggttcgcg gtcacctcgg tcagcggctc gaactcctgc atctcccggt
     1201 tgaagacatc cagggagaac ggtacctgct ccatgctgcg atcgcactcg ttcgcccagt
     1261 catagccctt cgccggcttg tccaccgggc aggagaattt ctggaccagg atggcgccgg
     1321 tgtccttcgt caggtgctcc ggcaccagga agaagtgaca ggtcaccgtc tgaccgttcg
     1381 ccaatgtcac cggcgccgat ccgttgatcg tcttcacatc cgccggggcg tccgcgttgg
     1441 taccgcaaaa gaggtacatg atcggcacgt tgtacggctt ctcgccgtac acctcgtact
     1501 tgcccgccgg aagatcggcg aaccgggtcc tgccaagctc acctgtgtcg ccggtgacgc
     1561 gcttgtagtt ctgggcgccc tcgacgcgga cagtcagtgt gttggtcagc gcctgatcgc
     1621 tcgtgcagct ttccgcaaag tccccgtacg ccgtgccgtt gaagctcggc gggcaggtgt
     1681 agctggtgac ctcaatactg gccggcaccg gctcgggcgg tgcaatgtaa ttgatgacga
     1741 tcaccgtcgt catctggccg acacccactc gaagccgtgc cgctgagctg ccgggaaggt
     1801 ccgggttgat ctctgtcagt acgtagagtc cctgcttgag cgtgacctga tagcgtccat
     1861 cctcgccggt cgagaactcg cccggtccat tgccggagcc gtcctcggcc accagcgtga
     1921 aggccgcttc gccctgcgta catcccgccg tcttcgcaag ctgggcattg ccctgcgcgc
     1981 cgccgaggaa ctgcgtgcgc tcaccctctt caccggccgg gcagatgaac ttctgtacct
     2041 gcaccgagcc ggtatccggc ggtggtgcgg tctcgaagac cacacgcaca actgtggtct
     2101 ggcccggcga gacgaccacc tcccgcgcct ggtcgttcag cttgtagccg tacggtgcct
     2161 gcgtctgggt caacgactcc gtccccgccg gcacaccttc gaagagcact tcgcccgacc
     2221 ccgcgctgca tgcggggtcc tgcccatcat tcgcgagatc gaagcaggct ccctgcaatg
     2281 gctgcccgag cgtattgaca gcctgcacga gcacgcggcc cgctttcaac gtatgcgcaa
     2341 actggacctc ggtctgctcg ttgagcgtga tcgtcacctc ctgatccggg gcaatctcga
     2401 agtcgggcga tggcgccagc aactcgcgga cggtgtaggt tccaaccggc agctcgggaa
     2461 agacaatgcg cttgtcgaca tcgtcatcgt tgtcgcagac acggccggtg agcggcgtat
     2521 ctccactgag gacctggtaa cagccgccgg agacctcggc accgtgctca tccagtggga
     2581 tcacgaccag cgatccgatt tccgccgccg gcggcgtcgc tgcattcgcg accgtcacct
     2641 ggagcgacga gcccgcgcca atggtgaccg gcctgctcgc ggcaacgtcg tacccgatag
     2701 gcgcgacggt ctgggccagg gtgtagtcgc ccgccgtaac gttcacgaag gaggtgcgcg
     2761 cgtctactgt gccgtcgtcg gcgtcgcaga acggcccgta gattgttgcg ccctcaagcc
     2821 ggtaacaggc gttattcagg cgctgggcag gatctgcctc ctggacggtg atcaccgcca
     2881 ggcgaccggc ccgcgggagg ttcctgacct cgacggccgt atcctcgccc gcgacaatct
     2941 caaccgtctg cgcttcggcc cgttggtact ggttcgatgg cgtgcgtgtc tcgacaagct
     3001 ggtagctacc agcggcaaga tcggtgaaga cgagcacacc gtcgttcgga ttcgcgtccg
     3061 tgtcatcaca gatcgacaag atcgtcgcgt caccctgctg caccgcgaaa caggagcctc
     3121 cgagcgcctg accgttgccg tcgagcttcg tcacggtgag cgaacccggc tgcgggactg
     3181 cctcgacagc cacctccacc gacgcggtct cccccgcgac gacggtgaga tccaccggca
     3241 ctgccacctc gtacccggag ggcgcggtcg tcatcgtggc cgtccaggcg ccgggagcga
     3301 catccagcag ctcgatcaca ccctccgtac catcgttatc cgtcgtctca tcgttatcgc
     3361 agacggacat gaccccaccc ggaccggaga gttcgtagca ggcacccgcc agattggtcg
     3421 ttccgtctgt cgtaacgatg cgcaccgtcc cattttgggg cagcaatggc acgatgagga
     3481 tggcactgga gcgctccccg gcgatgatct cgaccgagcg aggctccaca ccgccgacat
     3541 cgccgcctgg aacctccgtg tcctcggcaa gagagacatt ccaggcaccg acaccgacgc
     3601 cgtcgatacc gatgatgccc gcctggtcgt tggtatcgcc ctccacgttg tcgcagaagg
     3661 gacctgtcac gctcgcatct gcggtgctgg tgagcgtaat gcacacgcct ggcaccagct
     3721 cgccgctctc cagctgaacc gtgacatcga gtccaccggt tgtcggggcc acacgtgcca
     3781 gttcgaaccg caccggcgtg accgtatcgg cctcgaccgt cacagtcatc ccctccgcag
     3841 gaacctggaa ttcctccggc accgactgca gggcgacggt atgctcgcca agagcgaggc
     3901 cgtctacctg gatcgtgccc ggggtggcat tggcgtctgt cccatcgtca tcacactgct
     3961 gggctccgcc atcgatcgta tagcaaacac ccgggaccga attgccatcc ggatcaacta
     4021 cctcgattga gactgatccg gtcaacgcca cattctggaa tgtcacggaa gcaggcgccg
     4081 cggcggtaac cgtcacgctc tgctcggctg cgagtcggta tccctccggg gccgttgtct
     4141 gggcaatcgt gtggttgccc acccgcacac cggtgatcgt cacggtacca tcgccgtcat
     4201 cgcagatagg ctcaccaccg tccagtgcgt agcaggcacc ggcaaggctc tctccggcgg
     4261 cattcgttgt cgtgatgtca atggatgaga cgacgacatc atggggcacc tggagagcag
     4321 cgttctcacc ggccgcgata tcgaccggta tcggctcgac cggctggtat ccctcgggag
     4381 cctgtgtctg ggtcagagta aacgtaccgg gaaccagtcc ctcgaactgg acctgcccat
     4441 cgcccgcctc tccgccgttc gtgccgtcat cacagagcgg gccaagagga gcgtcgccaa
     4501 cggtcagcgt ccagcacgcg cccgggaggg gttcacccgc ggcattgaca gcgacaatcg
     4561 gcaggacgcc cggctcgggc gtcgccgtcg ctgtcggctc aggggcctca gcggcaatcg
     4621 cgacccgggc cgtctcaccc tcaacgacgg agacctgcgc tggctctgcc tcgaccagct
     4681 cgtccggtcc ctccctcaca aagatggagt agttcccctc gggcacatcc tcaatgagga
     4741 tttcctcagg cgcaggcgcg gcgtcacctt ccccgttgtt acaggcattt ggggcttcaa
     4801 gcaggccgat gcctccagag gtgttcaact caacgcacac cggcccctgg gattgatcca
     4861 cagcggcgat gtccacgacc acatcgccgc ccgttgctcc ggcaacatcc tggggggcgc
     4921 cgggtggctc gacggtcgga gctggagtcg cggtgggctg cgctgtggtt ggcgccccta
     4981 cgataacagt attgcggtca cccgccggaa cctcgatctc ctggtccgct accggttggg
     5041 tgccatcggg caccgtggtc tggtgcagcg tgtaggtgcc agagggcaca ccgaagaatc
     5101 cggtgtttcc gttaccgggg aacgggtcat cgctgtcaca gacagggccg atggtctcat
     5161 caccacggac gagctcgaag catgcaccgc cgattggctg accggcctca ttctgcagcg
     5221 tgacgatgag atcgcccggt accgcatctt ccgctggtgc ctcggtcggt gtcgcctcgc
     5281 tcgtcggctc ggcggttgga gcaaccgtgg ggacctcagt gggaacgttc gttggcgttg
     5341 gagtcggaat ctcggtcggg acgtcagtcg gcactgcggt cgggatcgcc gcaccctgca
     5401 cacggacttc cgagacctca ttaccagtca cctggaccgg cgcgtcggca gccgggttgt
     5461 agcctgccgg ggttcgcgat tcgtgcaggg tataggagcc cgccggcaca tcgaagaacc
     5521 caatgcgccc gtcatcggca acgtcaccgt cctcatcgca gacagcctcg cctacaacgc
     5581 cgccattgct gtcgagcagc tcgaagcagg cgcccccgac cgggttgccc tgggcatcct
     5641 ggcggagcac cacgacattc ccggtcggcg ccggggtcgg ctcggccgga gccgccgcct
     5701 gcactaccac cgtctcctca ccaccggccg gcacctggac ttcgcggtct ggcgagggct
     5761 ggacaccttc cggggccgtc gattggtgca acgtataggt gccggacggc acaccgaaga
     5821 aaccggtgtt gccattattg gggaaggcat cattaaggtc gcatgcctcc gccttcacct
     5881 cgccaccctg caccaactgg aagcaggcac cgccgattgg cgtgttgttc tggtcctgaa
     5941 gcgtcacgat gagattgccg gggacggcag ccgccggtgc ctcggtcgtt gcggtaggtg
     6001 tcaccgcgac atcgtcaccc tgaggtgcct cggtcgcttc gccgatcggc aggatcccgc
     6061 cgtcctcggc agtgggctca tcctccggct caccggtgct gatctggatg ctgttgtccg
     6121 tctcctggag gtccacttcc tggtctggtg cctgctcggc tccttccggc gccctcactt
     6181 cggcgacggt ataggatccc ggctccaggc cctcgggaaa cctgaggaga atcgagccgt
     6241 cggcgacgcc atcgtcggta tcacagccac ggttgatgac cgtgccaccc tcccgtacct
     6301 cataacagcc gccgggaagc ggttgacctg tcccaccgtc cacccgtgtg atcgtgagcg
     6361 tcccgtagcc aagatcctcg gcctcgatca cggtaccgga ggcatcgaga gtcacgcgga
     6421 ccggcccctg tggcgcggtg atgacctgcg ggttaccgac cggcacgccg tctatctgca
     6481 gctcggcatt gaccggtccg ggctgaagcg cctgcacgat tgccgtcagg gttccgtcgt
     6541 ccgggcgcag tgcatacgtg ccggcgtctg tcgccagggt cgcaattgac gcgaccggga
     6601 ctgcttccgc ctcctggcgc agggcattga agcggaagta gacgccagca tccgtttcgg
     6661 cgatggtcac ggtatcggtc tggtccgggc cagactcgct caggaagacc tgttggccat
     6721 cctgcgtcat cgccgcgatc tgccactgcc actggccagg aggaatcggg aagacgcccg
     6781 tccagatgcc ttcatggttg gtcagctgcg taccggggca attgatatcg aaggggttac
     6841 aaccgaactg ctgttggaaa tccccgatgg cggcgacctc ggtgaagctc gccgtgctga
     6901 ggtcctggac ctgggtttgt gcgaggttgt tctgcgcccc aggtgccgcc agggcgcggc
     6961 ctgcatccgt caatggcaga aagagtccga tcagcatcgc gatcgcgccg aagaggtgca
     7021 ggaacctgcc gaccccgcgc atcgtgatcg cgcggcgcgt gctgcgcctt cgggccgtcc
     7081 cgaatcgtgc ccggccgctg acggcttcca ttgcggatcg tgaacgcgtc tctgacatgg
     7141 catctctccg ccgtggctgt tgctgatcca tcggggatct tccccccgat ttcgacccct
     7201 ggacgagtgc gtgaccaatc atgtgtgttt cccggattct tcgtctgatc ataccccgct
     7261 cggacccgtt tgatattcac cattcgcatc ggggaagacg gacgcagttc aggctctttg
     7321 cttcccctcg gaacccggga cccccggatc gctcgcgcca ggcgcgtcac gatccgggca
     7381 tcgccgggga gaggcagagg gatgggaaag gggtatgtgg gaccagttgt ccgagtgttc
     7441 tgcctctcgt gtttgagaac gcgcagggaa ccggattggt tacaggctgc gtggagacgt
     7501 aatgcgtagg gggcatccgg cagagtccgg tcgtcatgtc gacaagatgg cccccacgtt
     7561 caagccgttc gttatcccag gaagagtgtc agggctatgc tgtccttggg ggcctgttcc
     7621 ccctcacccc ttcatcctgc gcgaagcgcg ttgacaactg ccccctcagg gacaggaaag
     7681 cccgttcgcg ccgggttctc tttggccttc tacgttctgg attcacggga gaaaggtgat
     7741 tgggagcccc cctcggaacg acgagctaat aagcgggagt ggtgactggg tagatattcc
     7801 cataacccca ccggttccga agactcgagg tctcccactc tgcgcaacga gaaggtatgg
     7861 cgaggagcgg gacagcacag gaccgtcccc taccactaga tgagcccgag tctattgatg
     7921 gacatccgct gcccttccgg ctccagcttg ctatctcagc gaggccacga gatcctccag
     7981 ctcgtccgcc gtggtcccga tgggaggggt cgcaaggaag acatgcgtta cgccggcctc
     8041 gtgcatctgc ttcagacgtg cccggacatt cgccagggac cccgcgacaa caagctcctc
     8101 catcaatgat gtcggaatga gcttcttcgc cgcctggagg tctccgccac gtcgaagcgc
     8161 gttgaatccc ccgccttggc tgagaatctc ctgcgtacgc atgacgtcgc ggacatccct
     8221 gatgatctgt tcgacgtcat acccttccgt gaccatcagg cgctccatct gcggcagcgt
     8281 atggatcatc gcgacggtgc tcttgcgccg ctcgtagacc ttctccggat cgtccgtgac
     8341 catgacctgg gagcgcgcgg caaagagaaa gcctgtgaga tctcgcccga gccgctccgc
     8401 ttcaccgcgg acggtcgcaa tcgcttcgcg catgaattgc atggaggcga gatcattgaa
     8461 gatcacaccg tccgcgtgcc gggcggcaag ccgcatcgcg atcgggccaa ctgccgccag
     8521 gtagatgggg aaacgcgact gcaacgggcg gaagacccgc tcccagtcgc gaatggcaaa
     8581 ctccgtcgcc tcctccggtg aattcgcaac aagttccggc gaccaccatt gacgcaggag
     8641 gtcaaacgtc tcggcaaggc gtcgggtcgg cttgcctacc tccagcccca tcgcgatctc
     8701 ataccactcg ctctgccccc ggccgagccc gagaaatgca cggccatcac tgagcagatc
     8761 cagcgtggac atcgatgccg caatcaggct gggatgccgg tagtatggat tcatgacccc
     8821 ggtgccgagc cggatcttct ccgtctggcg ggcaatggct ccgagtaccg tggcggcatc
     8881 ccaaccgtca ggatcctcca gcagccagag cgattcgata ccagcccggt ccgcgatgcg
     8941 gccgagttcg atcgtccagt tgactgcccg ggcaatcgcc gcgcggttac cctgtccctg
     9001 ggccctcgcc cacggcgcgg gagaattgtc caggcaatag gccaggggag ctgccgcttc
     9061 agccaatggc cttactccat tccaccgtcg acccagtcca gaaatgagag cacttcctcc
     9121 catcctttgt catcggccag cgcctcctcg gagagcatcg tgtcgcggac ataccggctc
     9181 aacgacagcc gtacttctgt ccctgacatc ttctcaacct cacgaacgca cctgcgatgc
     9241 aatcgttcga catcggcgct cgtgtatcgc acatacgctt ccatgttgaa gatgttctcg
     9301 agcgacgacc aaacctcctg gggttccatc tcacctgcct ccgaatgatg acctgaaccg
     9361 acttacctga tgggccctga ctgctcccac ggcactctta attgtatgag gaaggtaatt
     9421 tggactcctt ttagtgctgg ttcccggcgt ggtgagcgtc cagcatcaag gacgtgttcg
     9481 tgcatgtgtc aatacgggcg ttcgattgct ccagcccgac ctattgacac attgttgcac
     9541 gagggatcaa caatcttcgc ggcacatttc gtccaagtgc gacatggatt gcaatgcgat
     9601 cgacgacgtt tgaacccacg gttccactac ccagacccgc catcgtaacg tgtgccattc
     9661 gggacgagcg gaatggttct acttctatca ccacctgacc ggcgaaagca tgcccgttgg
     9721 cttcggtctc tcttgcgaca ttcttggtaa cgatgggagg caaaatgaat ggacccttgg
     9781 atacggccca gtggctgctg ggactggacg ctgattcatt atcggtgtgg cagatggcga
     9841 tccgcgcact catcgtctac gctgctgccg taatgatggt tcgggttggg gagaagcgtt
     9901 ttctcggaaa gaacacggcg ttcgatgtaa tccttggtat catattgggt tcagttgtga
     9961 gccgcgcaat cacgggttcg tccccgtttt tccctacact cggtgccggc ttcgtccttg
    10021 tagggttgca ctggagtatc gccgtgctcg cattccgttc agaacggctc gcgacgctgg
    10081 tcaaaggtga gtctcgattg ctgatcagag acggcaaggt gcagcaggag gctatccata
    10141 agagccatat tggtgagcgc gacctactaa gtgccctccg cgtgaacggc cagcttacaa
    10201 gcgtgagcga tgtggcggag gcacacctgg agcgttcagg tgatatcagc gttatcgagc
    10261 gcgaaacgga gcgtgagcca gtgatccttg acgtggccgt ggaggatggc gtccaaaccg
    10321 tgcggatcaa actcggctag aacgctccag gaacactgaa ctgcgagggt agaaagacag
    10381 gggagtatca ggcataacgc ttaccttcga cgggagttga ccagcccgca tgccccaggg
    10441 cacagaacca ggcgaagatg tcgccgtttg cctcgtggaa ccactgcaca ttaggcacac
    10501 cgaactgatc gaatgagacc cgaatcgcga agccgaacga cgggaggccc agcatggtgg
    10561 cctcggggga ataaacttca aaagcatggc tccggcatcc cagacgagca gtcatcgtgc
    10621 tatcttcatt cgggaagacg gtgagaaccc agccgctttc gaaggttgtt tcgtagcccg
    10681 cgccgcgcgt ctcatgcgtt tttgacccga gttgctttct gctcatgtcg aactcccgcc
    10741 tgaaactgct caccccgcat ggatgacgga ggcttcgctt ccataaagta tggagttcag
    10801 gtagcaggag gcgttcctcc agacgaactc tcttgactga ggagcgcttc aggcatcagg
    10861 atccgatgag cgatgagcgc cgctgcccgg ctcgattgac gtcttggcat caacctcagc
    10921 cgtgtgatcg gccttcgcct cctcgtgggc caggtcgtcg cttgaagagc gtcgggcaat
    10981 ttcctcaagg tgctgttccc gaccatagat gacgaccgta tcgccttcgc tgaagacgta
    11041 gtctgcggga ggtgccccga catacccacc ggacggctgc acgatcccga gaatcaggag
    11101 gccttcggca ggcaaatcga gcgtgcaaag ctgctgacct ttcagccagc ttccactccg
    11161 cacctccact tcagcaacac ggtaatcctc gcgcagatga aggagtgccg cgtaatcgcg
    11221 caagaccagc gtggttgagc ggctcaatgc cctttgaatc actggcgtca gcatgcggtt
    11281 gaaccaacga ctgcgagtca atgcgaccag tcctaccagc ccggcaacca gaaccagaaa
    11341 gcgttccggc gctccatcct ccgatcccgt aaaggagagg atcagcgaag caaccaccgt
    11401 gatggcaccc gcattgccgg ccctgatgag caaggaaatg a
//
LOCUS       DASYWM010000117        12628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_248682, whole genome shotgun sequence.
ACCESSION   DASYWM010000117 DASYWM010000000
VERSION     DASYWM010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12628
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248682"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..617
                     /locus_tag="VGR29_04820"
     CDS             84..617
                     /locus_tag="VGR29_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2072947.1"
                     /translation="MREYQKVPFLAAADASGEPLETSQDGRSPEVIVDVLESMVVSLE
                     RLISDRSADELQQAARDGGWGAVELLAHMRDWEEISRERVRRMLEEDPPVLEEHDDSL
                     WAIEHDYGTQDGHEAVRQFADLRRSLVERLRNLDDDEWQRTAVLPSDGEITLMWLMSN
                     LTRQDGRHLDQIREALG"
     gene            750..1373
                     /locus_tag="VGR29_04825"
     CDS             750..1373
                     /locus_tag="VGR29_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2072948.1"
                     /translation="MRTGSEYVDVDAPVSAEQLEAIRSLDNNALIIRLHFTINHLSRW
                     LSPIHEPAPLIRSRYRGEPTVKDLLLAMRDYEQYVYPKMYVIASQETPDLDTIPDYRP
                     SAARQVSDAEHSPIVLMSNFRRLRQSTCSLLRNLPDEAWDRKGFSRVHRNATIRQLAE
                     GLAEHDYRHLRAIDQLLTETGAREGLAEIQKAPLDELIKLVPQSMKG"
     gene            1440..1865
                     /locus_tag="VGR29_04830"
     CDS             1440..1865
                     /locus_tag="VGR29_04830"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV2072949.1"
                     /translation="MDSSRHSASGLSETEIVEALGREFFLALDARDPRRLRNTLAANA
                     TFRAHPHSAPIRPADEIVAYFGTVVSSYPNAHWDVTDVIAAPDRGSVQFVIREYSAQQ
                     GRERISEQVAIIRVSGGKIVSVVGYYDSSEFQRVFWEAD"
     gene            2244..2888
                     /locus_tag="VGR29_04835"
     CDS             2244..2888
                     /locus_tag="VGR29_04835"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV2072950.1"
                     /translation="MSESYESDAPAIPDIVIRRLPIYVRTLKGLTDREIGSVSSDELA
                     ELIGVTAAQIRRDLSYFGKFGKQGKGYDIAFLAKEIDRILRLDRQWPVALCGMGKLGH
                     AIANYRGFSPSSFSIVALFDRHADRSGEQVNDLEIRPIDDLVETIQREGIKIGIIATP
                     KIHAQETAELMVEGGVEAILNYAPVILKVPEHVTVREIDPVSALQSMTYYIDRD"
     gene            2881..3243
                     /locus_tag="VGR29_04840"
     CDS             2881..3243
                     /locus_tag="VGR29_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2072951.1"
                     /translation="MTSTRLHHVQVAIPHGGEDEARRFYGDLLGLTEIPKPGNLARRG
                     GVWFQTGNLQLHLGVDSNFVPASKAHVAYEVEDLDAVRDRLEAAGIAIVNDEPLPGFD
                     RFYVTDPFGNRVEILEPQ"
     gene            3391..3903
                     /locus_tag="VGR29_04845"
     CDS             3391..3903
                     /locus_tag="VGR29_04845"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV2072952.1"
                     /translation="MEADKEILTKEQLLTEMDRAWSDLQATISGVEASDLTERTDHVG
                     WSAKDHLTHLSAWANSVLVMIRDGRPQHEGLGIDKATYETDGYDHENEVIRQSRAGMS
                     LPDVQANLEHVYREFMRVIQGMSDEDLHKPCSAFIEGGEEVEIFQKISGDTWSHLDEH
                     REYIERILAD"
     gene            complement(4092..4808)
                     /locus_tag="VGR29_04850"
     CDS             complement(4092..4808)
                     /locus_tag="VGR29_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEV2072953.1"
                     /translation="MKLADFDALSFDCYGTLIDWEHGLREGLRKVAALQSPSPDVDAL
                     LESYADAEAEVERANPGMRYPQVIAESVARIGNAHGIAVPLEVGVEIGASVSDWPAFP
                     DSTEALARLSQRYKLIILSNIDRASFAGSNERLGVTFDAILTAEDIGSYKPDPRNFEA
                     LLEQVQGMGISRDKLLHVAQSLFHDHVPAKAAGLSTVWIDRRGSKPGYGATPIPENTV
                     RPDWTFPSMAAFANAVKTED"
     gene            5212..6306
                     /locus_tag="VGR29_04855"
     CDS             5212..6306
                     /locus_tag="VGR29_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEV2072954.1"
                     /translation="MAETTTSRGVIARKPGEPGVIEEFTIDAPQAGEALVRILASGVC
                     HTDLSAKNGVFGTSGFPFLLGHEGSGIVERVGEGVTNVQQGDYVILAWRAPCGQCRFC
                     NVGKPHLCAASLNAHQGMRTMEGELLTPILGIGTFCTHTLVHARQCIPVDKTLPPEQM
                     SLIGCGVMTGVGAALYSARVEPGQSVAVIGCGGVGDSIIMGARLAGATTIIAVDIDPG
                     KLEWAKEFGATHTLDARQGDPVARIKEITGGNGVNASFEAVGRAETIETAIMCRDLAG
                     TCVIVGVADQQATLHVPVAKFFDIGGSLRVSWYGDCLPTRDFPLLADWYAQGQLDLDR
                     VVTRVITLEESEEAFHAMERGETLRSVIRL"
     gene            complement(6629..7714)
                     /locus_tag="VGR29_04860"
     CDS             complement(6629..7714)
                     /locus_tag="VGR29_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HEV2072955.1"
                     /translation="MSTESVSPIDTLIELMALPGPTGQEEPVLSWCRERWPALGAEVR
                     VTPIGNVVAHVGGTGPRLLLQGHADEIGFVVRSIDSRGFVWLADAQGSNRAFHARYPV
                     GQPALILARSGRIPGVFATPTGHILSTRDDRGAELPDTAGTIFVDIGAESREEAEQMG
                     VHVGAGVIWNPVTQRLGSRYIGKAIDNRVALALMTHVLMEIEKSELAYELTLAATVQE
                     EIGLVGAQSLVGSGTYDLAIAIDNGPIADYPGTELGDLPVRLGHGPTLVYKDAMVHYD
                     RRIIERLLATATTSAIPVQQGVYPGFGSDGVALIRAGVPTALLAISTRYTHSPFEMGD
                     VRDLEGALRLLTAFITTPAEPLPLGPS"
     gene            complement(7842..8132)
                     /locus_tag="VGR29_04865"
     CDS             complement(7842..8132)
                     /locus_tag="VGR29_04865"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV2072956.1"
                     /translation="MVLETAMLDVRPGQEAEFEHAFVPASAIISSMPGYLGHELHRCI
                     EHPSRYLLLVRWETLEDHTVGFRDSPKYQEWKRLLHHFYDPFPTVEHFTRVR"
     gene            complement(8137..9795)
                     /gene="guaA"
                     /locus_tag="VGR29_04870"
     CDS             complement(8137..9795)
                     /gene="guaA"
                     /locus_tag="VGR29_04870"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV2072957.1"
                     /translation="MATTDPTTLPTTDPTEGEVASAHSVEESAHLLEDAPTAVDAVVI
                     LDFGSQYSQLITRRVRECGVYGELMPHDATWEEVSKLQPKAVILSGGPSSVYAEDAPQ
                     LPEWVLEQNLPTLGICYGMQLLAHALGGTVASADRREYGTATIEIANSDDVAGLFDGM
                     PERLDVWMSHGDHISKLPDDFKVLATSPNSPVAAMGHSTMVGLQFHPEVQHTTNGTQL
                     LRNFLTKVAGCTTNWTPESFIDASIREIREQVGEGRVLLGLSGGVDSSVAAALIHKAI
                     GDQLTSVFVNNGLLRLGEAELVQETFTRAFDTNLVYVDASERFLEKLKGVSDPEEKRK
                     AIGETFVRVFESEAEKAGPFEFLAQGTLYPDVIESATQSANSKTAAKIKTHHNVGGLP
                     ADMQFKLLEPFRFLFKDEVRAVGRTLGLPEEIVERQPFPGPGLAVRLLGEINATDLEL
                     LRKADAIVREEIEAAGHAKDVWQYFAVMLPINSTGVMGDYRTYARVVAVRAIASQDAM
                     TADWARLPHDVLGRISNRIVNEIDGINRVVYDITSKPPATIEWE"
     gene            complement(9995..10444)
                     /locus_tag="VGR29_04875"
     CDS             complement(9995..10444)
                     /locus_tag="VGR29_04875"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEV2072958.1"
                     /translation="MMMPGTTTVRLTLPLPPSVNAQYVTVGRKRVLSKVARTFKKDAS
                     KVIALLQERSKITPVEEKALANNLLGVYMTFYFETPMRRDLDGGLKIALDTIATSLGF
                     DDRNVVDLHLTKQIDPLHPRLEIEIETISEWTFDRSYVYLGDESDDA"
     gene            complement(10441..11388)
                     /locus_tag="VGR29_04880"
     CDS             complement(10441..11388)
                     /locus_tag="VGR29_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2072959.1"
                     /translation="MEHLAEHHIPDAIALAHEASVREQRHVPALPRLDVRDVLERAVG
                     DIVRHGTGIAALDGSKLAGYMSFIGPIDNFFGTSPGCFSPLHGLAATGPQRRRLFSLL
                     FQHASGLLTAKGAKVFAITTYAHDHDAATALSLNGFGIRNADAIREIATPLETVIVPD
                     ITYREIPWSDAGRLLPLKNGLVEHLHRSPTYVAAEAFTPESFALLCEERKSRFFIAQD
                     GDTPIGYMEITDEGESYLTSDPGMLNICGAFLHEQYRGQGIYAQLLGVVIDTLRKEGV
                     RHLGVDFETMNPTALHFWTRYFDPYTYSLARRLDDLPES"
     gene            complement(11411..>12628)
                     /locus_tag="VGR29_04885"
     CDS             complement(11411..>12628)
                     /locus_tag="VGR29_04885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV2072960.1"
                     /translation="AESPAMSSTVESPATIEVTIDRIVGDGKGIGFVDGKTIFVTRTA
                     PGDVVRARVTRQQGSTVHAVIDEIITPSPMRIQPDHPHAGSCVGCDLMHLSYVDQLAT
                     KVSIIQDSLRRIAKLDEIPEIPIRPSENQWGYRSRAEWQVNVPNKVVGYYAGGTRRIV
                     DVEVDPVSTEGLNTLLTTLRDDVSAGLVPENAKEYRAVSTETGYALEPTTTTRSAILQ
                     RTIGGESYHFSAECFFQANIPVTEKLVADVTKIADIARQQPGIAIDLYCGVGLFTVPL
                     AHRYKRVIGVESFKPAVKFANDNLEAAGLTKTKIMSMPVEHWVTQDQSRHGRVSLVVF
                     DPPRTGAGAKTVQGILRLNPAHIAAVSCNPATFARDLRGLLDGGYELVNITAYDMFPQ
                     THHVELLAHLRRS"
BASE COUNT         2418 a         3794 c         3941 g         2475 t
ORIGIN      
        1 gtgcatgacg atgcccgggc ttgatcctcg tccggccgga gggcaggcag ggatgagccg
       61 gatcaagtgg aaaacaggag ttcatgagag agtatcaaaa ggtaccgttc ctggcagctg
      121 cggatgcgtc cggtgaaccg ctggagacgt cgcaagacgg gcgatcgcca gaggttatcg
      181 ttgacgtcct cgaatccatg gtcgtttccc tggagcggct catctccgat cggtcagccg
      241 atgagctgca gcaagctgcg cgggacggag gctggggtgc ggtcgagctt ctcgcacata
      301 tgcgggactg ggaggagatc agcagggagc gtgtccggcg catgctggag gaggatcccc
      361 ccgtgctgga ggaacatgac gattcactct gggccatcga gcacgactac ggcactcagg
      421 atgggcacga ggccgttcgt caattcgccg accttcggcg cagcctggtc gagcgtctac
      481 gcaatctgga tgacgatgag tggcagcgta cggcggtcct gccgtcggac ggcgagatca
      541 cgctgatgtg gctgatgagc aacctgaccc ggcaggacgg gaggcatctg gaccaaatac
      601 gggaggcgtt ggggtaatgg catcggcgcg ggccgtgcga taatactctc cggagtagca
      661 ccgcgatccg tacgtgggtg gtcccgggca ggtgggtcgg cgcgctacgg ggtagaacag
      721 gcaacattcc aagcaagagg gcaggtggca tgcgcacggg atccgaatac gttgatgtcg
      781 atgcaccggt gagtgccgag cagttggagg cgattcgctc gctggacaac aatgcgctga
      841 tcatccggct gcacttcacc atcaatcatt tgtcccggtg gctttcaccg atccacgaac
      901 ctgcccccct gattcgctcg cgctatcgag gtgaaccgac ggtcaaagac ctcctgctgg
      961 ccatgcggga ctacgagcag tacgtctatc ccaagatgta cgtcatcgca tcgcaggaga
     1021 cgcccgatct ggatacgatt ccggactatc gtcccagtgc cgcccgccag gtttccgatg
     1081 ccgagcattc cccgattgtc ttgatgtcaa acttccggcg cctgcggcag agtacctgct
     1141 cgcttctgcg caacctgcca gacgaggcat gggatcgaaa ggggttcagt cgcgttcacc
     1201 ggaatgccac aatccgccag ctggcggagg gactcgcgga gcacgactac cgacacctgc
     1261 gtgccatcga tcagttactg accgaaacag gtgctcgcga gggcctcgcg gagatccaga
     1321 aggctcccct ggatgaactg attaagctcg ttccccagtc gatgaagggc tgatcggctt
     1381 catgatggct ccccttcgtt atcgttcaag tacctttgct gaatagccag gaggcgaatg
     1441 tggattcctc acgacacagc gcttccggcc tctccgagac cgagatcgtc gaggcgttgg
     1501 ggcgagagtt ctttctcgcg ctcgatgccc gggacccgcg acgactgcgc aacacgctgg
     1561 ctgccaacgc gaccttccga gcccatcccc acagcgcacc tatccgtccg gcggacgaga
     1621 tcgttgccta cttcggcacc gttgtctctt cctacccgaa tgcacactgg gacgtgacgg
     1681 acgtcattgc ggcaccggat cgcggatcgg tgcagttcgt tattcgtgaa tactcggccc
     1741 agcaggggcg ggagcgcatc tccgaacagg tggcgatcat ccgcgtatcc ggtggcaaga
     1801 tcgtcagtgt ggtgggctat tacgatagct ccgagttcca gcgggtgttc tgggaagctg
     1861 actaggtccg taggcagcta caagactcaa actgctcatg ggctcccttt gacaacggtt
     1921 catactgtat acagtatggc tgctcgcggc cttgcaggtc ctgtgcttgc tcggattata
     1981 attgtgaata acagcacgaa catggaacga ggggagtcct cgccatggag cgtgcccgat
     2041 agagggatca tcgccagcgg taagcatgcg tcccgtaacc tggtatccgc agcgagtgcc
     2101 ccgggaagaa catgacaagc aattcaccag gaaccaacgg gaagtcagat catatttcag
     2161 ggcaagggtc agcattcgag gatcgatacg cgaaccggtc gacgtcttcg ctcctcgaac
     2221 ccgtaccagg atcactatcg cggttgtcgg aatcatatga atccgatgcc ccggccatcc
     2281 ctgatatcgt gattcgccgg ttgccaatct atgtccgcac attgaaggga ctgaccgatc
     2341 gcgagatcgg ttctgtttcg tctgacgagc tggccgagtt gattggtgtg acagcggcgc
     2401 agattcgccg ggatctttcc tatttcggca agttcggcaa gcagggcaag ggctacgaca
     2461 tcgcctttct cgcgaaggag atcgatcgta tcctccggct ggatcggcag tggccggttg
     2521 ccctctgtgg catgggcaag ctgggacacg ccatcgccaa ttatcgcgga ttcagcccgt
     2581 catctttttc gattgttgcc ctgtttgatc gtcatgcgga ccgctccggc gagcaggtca
     2641 acgatctcga gatccggccg atcgatgatc tggtggagac gattcagcgc gaggggatca
     2701 agatcggcat catcgcaacg ccgaagatcc acgcccagga gaccgcggag ttgatggttg
     2761 agggcggtgt tgaggcgatc ctcaactatg ctccggtcat tctcaaggta ccggagcacg
     2821 tcaccgtgag ggagatcgat ccggtctccg cgctccagag catgacctac tacatcgatc
     2881 gtgactagca cgcgtctcca ccatgtacag gtcgccattc cccatggtgg cgaggacgaa
     2941 gcgcgccggt tctacggcga ccttctgggt ctgaccgaga tcccaaagcc cgggaacctg
     3001 gccagacggg gcggtgtctg gttccagacc ggaaacctgc agctgcatct tggcgtggac
     3061 agcaatttcg tcccggcgag caaggcgcat gtcgcctatg aggttgagga cctcgacgcc
     3121 gttcgcgatc ggctcgaggc cgccgggatc gcgatcgtca atgacgaacc gcttcccggc
     3181 tttgatcgct tctacgtcac cgatccgttc ggcaaccggg tcgagatcct ggaaccgcag
     3241 tagcaaacaa tcccgcgaag ccccggatat cgcatccggg gttcttctgt gtgctccgac
     3301 acgaggcaca aggctggtgg actgaacgga aatggcggac acattttgac ctcagttacc
     3361 agtaagatgt gtggcaagga ggaaacaacc atggaagctg acaaggaaat cttgacgaaa
     3421 gagcagttgt tgaccgagat ggaccgtgcg tggagcgatc tacaggcgac gatctcggga
     3481 gttgaagcca gcgacctcac ggagcgaacg gaccatgtag gttggtccgc aaaggaccac
     3541 ttgacccatc tctcggcatg ggccaactcc gtgctcgtca tgatccgtga cgggcgaccg
     3601 cagcacgaag gcctcggtat cgacaaggcg acctacgaga ccgatggtta cgatcatgag
     3661 aatgaggtca tccgccagtc gagggccggg atgtcgctgc ccgatgtcca ggccaatctg
     3721 gagcacgtct atcgcgagtt catgcgggta atccagggaa tgtcggatga ggatctccac
     3781 aaaccctgct cggcattcat tgaaggcgga gaggaggtag agatcttcca gaagatcagt
     3841 ggcgacacct ggagccatct cgacgagcat cgggagtaca tcgagcgaat cctggccgac
     3901 taacgtggtg atcgaaagaa gtgcttgctg taggggccgc tcccgctcag tcacgacgtg
     3961 actgctcatc gtgaagccag ggcggacgag catctgttgc cgttgaccac cctcatgtgt
     4021 aacgccgctg tagcccgcgc tttgcgaagg gttcaacaca cgcaatctca acgcaattga
     4081 agcgggagcc atcaatcctc ggtcttaacc gcattggcga acgccgccat tgacggaaat
     4141 gtccaatccg gccggacggt gttctccgga atcggggtag cgccgtagcc cggcttgctg
     4201 ccccggcgat cgatccagac cgtgcttaga ccggcagcct tggcaggcac atggtcgtgg
     4261 aacaggctct gtgccacatg aagcagcttg tcccgcgaaa tgcccatccc ttgcacctgt
     4321 tccagcagtg cctcgaagtt gcgcggatcc ggcttgtagg aaccgatatc ttcagcggta
     4381 aggatcgcat cgaaggtgac accgagacgc tcattgctgc cagcgaagga tgcccggtcg
     4441 atgttcgaca ggatgatgag cttgtagcgc tgcgaaaggc gagcaagcgc ctctgtcgag
     4501 tccggaaacg ccggccagtc ggacacggat gcgccgatct ccactcccac ctccaagggc
     4561 accgcgattc catgcgcgtt tccaatcctc gcgacactct ccgcgataac ctgaggatag
     4621 cgcatgcccg ggttggcccg ctcaacctct gcttctgcat cggcgtagct ttccagtagc
     4681 gcatcgacat cgggcgatgg cgattgcagt gccgcgacct tccgcaagcc ctcacgcagg
     4741 ccgtgttccc agtcgatcaa ggttccgtag cagtcgaagc tcaacgcgtc gaaatcggcc
     4801 agcttcattg agtccccctt ttgcaactga tggtaaccac tacgtttcgc ctgacatcat
     4861 acggctggcc tgccagatcg taatcagtca atgtccggag tgtctcgcgg gcgtgatggc
     4921 tgagctgttg cacgtaccgg tcttcgctct gtctcgattc tcgtctgcac cgatacaaca
     4981 agtatcgctg cctactgttt agggacaaag tgccccaaaa tcacggtggc tctggcattc
     5041 tggcccggtc gttcgccctg ccacgccaga atgccagaac caggcactgt agctgccagg
     5101 cggtgaccgg aggcccaacc cccgaccttt tatggtgtaa ttcctccagc attgggccca
     5161 aaacgtcaac ttggatggat cgctgaatct ttaccagatt ggagaacaag catggccgag
     5221 acaacgacct cacgcggtgt cattgcccgt aagccgggag agcctggggt aatcgaggag
     5281 ttcacgatcg atgctccgca agctggcgag gcgctggtac gcatcctcgc cagcggggtc
     5341 tgccataccg atctctcggc caagaacggc gtcttcggta catcaggatt cccgttcctg
     5401 cttgggcatg aggggagcgg catcgtcgag cgggtgggcg aaggggtcac caacgtccag
     5461 caaggggact atgtaattct cgcctggcgg gcaccatgtg gccagtgccg gttttgcaac
     5521 gtcggcaagc cgcatctctg cgctgcaagt ctgaatgctc accagggcat gcggacgatg
     5581 gagggcgagc tcctcacccc gatcctgggg atcggcacgt tctgcacgca taccctcgtc
     5641 catgcccggc agtgtatccc ggtcgacaag acattgccgc ctgagcagat gagcctgatc
     5701 ggctgtggcg tgatgactgg cgtgggggct gccctctact ccgccagggt cgaacccggt
     5761 cagtccgtag ccgtaatcgg ctgtggcggc gtgggcgatt cgatcatcat gggtgcccgc
     5821 cttgctggag caacgaccat cattgccgtt gatatcgatc ctggcaagct ggagtgggcg
     5881 aaggagttcg gggcgaccca caccctcgat gcccgccaag gcgatcccgt cgcaaggatc
     5941 aaggagatca ccggcggcaa cggggtgaac gcctcgtttg aggcggtggg tcgggccgaa
     6001 accatcgaga cggcgatcat gtgtcgtgac cttgccggga cctgtgtgat cgtcggcgtc
     6061 gccgaccaac aggcaacgct gcatgtaccg gtggcgaaat tcttcgatat tggcggaagc
     6121 cttcgcgtgt cgtggtatgg cgactgccta cctacccgcg actttccgct gctggccgac
     6181 tggtatgccc agggccagct ggacctcgat cgcgtcgtca cccgggtcat cacgctggag
     6241 gaaagcgagg aggccttcca cgctatggag cgcggcgaga ccctgcgcag cgtgattcgg
     6301 ctatagggga aaaccccctg ccataaggga atagctgacg gctgctttcc cgcgccgtag
     6361 gtgtgcgggg ccccgcacga accccgattc ttcatgtcgt agggaggact tggagcaccc
     6421 cgcggggagg ttggcgatat gccgcacctc ccctacgagg gctgagtgac accatacgcc
     6481 cttcatatca agaccccgca ttccgagcgc ggtcgaggaa tctttgcccg ggccggggcc
     6541 atgcttccct tgagccagga tcccttagag caatctggcc ccactgcgca tgccccgtag
     6601 gttcaggacg acgattgctg gtcaatgctt acgacggccc gagcggaagt ggctcggcgg
     6661 gcgttgtgat gaacgccgta agcagccgca atgccccttc gaggtcgcga acgtcgccca
     6721 tctcgaacgg ggagtgtgtg taacgggtcg agatggccag gagtgcggtg gggactccgg
     6781 cccggatcaa tgccaccccg tcgctcccga accccgggta caccccttgc tggacgggga
     6841 tcgcgctggt cgtggccgtt gccagcaggc gctcgataat gcgccggtcg taatgcacca
     6901 tcgcatcctt gtagacgagc gtcgggccat gcccgagcct gaccgggaga tcgcccaatt
     6961 cggtgccggg gtaatcggca atcggtccat tgtcgatcgc aattgcgagg tcatatgtcc
     7021 cggacccaac cagcgactgc gcaccgacga gcccgatttc ttcctgcacc gttgccgcca
     7081 gcgtcagttc gtaggccagt tcggatttct cgatctccat gagcacgtgg gtcatcagcg
     7141 cgagcgcgac ccggttatcg atcgccttgc caatgtagcg ggatcccagg cgctgggtaa
     7201 cggggttcca gatcacgccc gccccgacat ggactcccat ctgctccgct tcctcccgcg
     7261 actcggcgcc gatgtcgaca aagatggtgc cggcggtgtc cgggagctcc gcgccccgat
     7321 catcacgcgt ggagaggatg tgcccggtcg gcgtggcgaa cactcccggg atgcgtccgg
     7381 agcgggcaag gatcagggct ggctggccca ccggataccg ggcatggaac gcgcggttgc
     7441 ttccctgggc gtccgccagc cagacgaacc cccggctatc aatactcctc acgacgaagc
     7501 cgatctcatc ggcgtgcccc tgaagaagca ggcggggacc ggttccaccc acatgggcca
     7561 cgacattgcc gatgggcgtg acccggacct ccgcgccgag ggctggccag cgttcccggc
     7621 accaggacag caccggttcc tcctgcccgg tggggccggg aagcgccatg agttcgatca
     7681 atgtgtcaat cggggaaacg gactctgtag acatggttcg gtatcactct ccctgatgcg
     7741 tggtgtggcc tgcacagccg gccgtaagtt gtaaagaccg gcaggaacag ggagatgctc
     7801 ggcagggacg acatctcatt gttgtctgtc agaacccggg atcatcgaac tctcgtgaag
     7861 tgctccaccg tcggaaatgg atcgtagaag tggtgaagca gcctcttcca ctcctgatac
     7921 ttgggagagt cgcggaatcc cacggtgtga tcctccagcg tctcccaccg gaccagcagc
     7981 aggtaccgcg atggatgctc gatgcaccgg tgcagctcgt ggcccaagta ccccggcatc
     8041 gaggagatga tcgccgatgc cggaacaaag gcgtgctcga actcggcttc ctgccccggc
     8101 cgcacgtcga gcatggccgt ttcgagaacc acccccttac tcccactcga tcgtggcggg
     8161 tggcttggag gtgatgtcgt agaccacccg gttgatgccg tcgatctcgt tcacgatccg
     8221 gttgctgatt cgtcccagca catcgtgcgg caggcgagcc cagtccgccg tcatcgcgtc
     8281 ctggctggca atggcgcgga cggccacgac gcgcgcgtag gttcggtaat cccccataac
     8341 gccggtggag ttgatgggga gcatgaccgc gaagtattgc cagacatcct tcgcatgccc
     8401 ggccgcctcg atctcctcgc gcacgatcgc atcggccttg cggagcagtt cgagatccgt
     8461 tgcgttgatc tcgccgagga gccggacggc gagaccgggc cctgggaagg gttggcgctc
     8521 gacgatctcc tcaggcaggc caagggtgcg tccgaccgcc cggacctcgt ccttgaacag
     8581 gaagcggaac ggctccagga gcttgaactg catatccgcc ggcaggccac caacattgtg
     8641 atgggtcttg atcttggccg cggtcttgga gttggcgctc tgggtcgcgc tctcgatcac
     8701 gtcgggataa agtgtgccct gcgcgaggaa ctcgaacggg ccagcctttt ccgcctcgga
     8761 ctcaaagacg cgcacgaacg tctccccgat tgccttgcgc ttttcctctg gatcgctgac
     8821 tcccttcagc ttctcaagga aacgctcgct ggcatccacg tagacgaggt tggtatcgaa
     8881 ggcacgggtg aatgtctcct gcaccagctc ggcctcaccg aggcgcagca ggccattgtt
     8941 gacgaaaacg gatgtgagct gatcaccgat tgccttgtga atcagcgccg cggccacgga
     9001 ggaatcgacg ccgccgctga gccccaacag aacgcggccc tcgccgacct gctcgcgtat
     9061 ttctcggata gaggcgtcga tgaaagactc cggcgtccag ttcgtcgtgc accccgccac
     9121 cttggtcagg aagttccgca ggagctgcgt accattggtg gtgtgctgga cctcggggtg
     9181 gaactgcaga ccgaccatcg tgctgtgtcc catcgccgcg accggcgagt tgggggaggt
     9241 cgcgagtacc ttgaagtcgt ccggaagctt gctgatgtga tcgccatggc tcatccagac
     9301 atcgagccgc tccggcatgc catcaaagag accagcgaca tcatcagaat tcgcgatctc
     9361 gatcgtggcc gttccgtact cgcgccgatc cgctgacgcc accgtgcctc cgagggcgtg
     9421 cgcgaggagt tgcatgccgt agcagatacc gagcgtcggc aggttctgct ccagaaccca
     9481 ctccgggagt tgaggcgcgt cctcggcata cacgctggac gggccaccgg agagaatgac
     9541 tgccttcggc tggagtttgg agacctcttc ccacgtggcg tcgtgcggca tgagttcgcc
     9601 gtagacgccg cactcgcgga cccgccgggt aatgagctgg ctgtactgtg agccgaagtc
     9661 aaggatgaca accgcatcga ccgctgttgg ggcatcctcc agcaggtggg cggattcctc
     9721 gacggagtgg gcactcgcga cctctccctc cgtggggtcg gtcgtcggca gggtcgtcgg
     9781 gtcagtcgtg gccaaaaggg gtctctcctg gtgagcggaa accgcggtgg tcgatgggac
     9841 aaccgtcatt ggttggcatt ttacccgagg aagcccgctc gcagccccgc acctggtgtg
     9901 cagggtccgg cgcgtacggg cgaacttttt gttcgccctc ttgcccagca ggcctccggt
     9961 agttccccga taactgcttg tgcccggtgc gtctctacgc gtcgtcgctt tcgtcgccga
    10021 gatacacata cgaccggtcg aaggtccatt cagagatggt ttcgatctcg atctccagcc
    10081 ggggatgcaa cgggtcgatc tgcttcgtga gatgcaggtc gaccacgttc cggtcatcga
    10141 agccgagact ggttgcaatc gtatccagcg cgatcttgag gcccccatcg agatcacggc
    10201 gcatcggcgt ctcgaagtag aaggtcatgt agacacccag gagattgttg gcgagcgcct
    10261 tctcctcgac gggagtgatc ttgctccgct cctgcagcag cgcgatcacc ttgcttgcat
    10321 ccttcttgaa ggtccgcgcg accttgctca ggacgcgctt tcggccgacg gtgacgtact
    10381 gggcattgac gctcggcggc agtggcagtg tgaggcgaac ggtagtggtt ccaggcatca
    10441 tcatgattct ggaaggtcat ccaggcgtct cgcgagcgag taggtgtaag ggtcgaaata
    10501 cctggtccag aaatgcagcg ccgtggggtt catcgtttcg aaatcgacgc caagatgccg
    10561 gacaccttcc ttgcgcagcg tatcgatcac gacaccgagg agttgcgcgt agattccctg
    10621 gccacgatac tgctcatgga ggaacgcgcc gcagatattc agcatgccag ggtccgatgt
    10681 cagataactc tccccctcat cagtgatctc catgtacccg atcggcgtgt cgccgtcctg
    10741 ggcgatgaag aagcgggatt tgcgctcctc gcacagcagg gcgaacgact ctggcgtgaa
    10801 cgcttcggcc gcgacgtagg ttgggctgcg gtgaagatgc tcgaccaggc cgttcttgag
    10861 tggcaggaga cgcccggcgt cggaccatgg aatctcgcgg tatgtaatat ctgggacgat
    10921 gacggtctcc aggggagtgg cgatttcccg gattgcgtcg gcattccgga tgccgaaccc
    10981 gttcagcgaa agtgccgtcg ccgcatcgtg atcatgcgca taggtggtga tcgcaaacac
    11041 cttggcaccc ttggcggtca ataacccgga tgcatgctgg aacagcaggg aaaagagacg
    11101 ccgtcgttgc ggtccggtcg ccgcaaggcc gtgcaagggg gagaagcagc cgggggaggt
    11161 accgaagaag ttgtcgattg gtccgatgaa gctcatgtag ccggcaagtt tggacccatc
    11221 cagggccgcg atgccggtac catggcgcac gatgtctccc actgcgcgtt ccaacacatc
    11281 cctcacatcg agcctgggga gtgccgggac atgccgctgc tcacggacgg atgcctcgtg
    11341 tgccagggca atggcgtccg gaatgtggtg ttcggcgagg tgctccatgc gcactggcgt
    11401 actcccctcg ctaggaccgg cgcaggtgag cgagaagctc aacatgatgc gtctgcggga
    11461 acatgtcgta tgcggtgatg ttcactagct catagccacc gtccagcaat ccacgcaggt
    11521 cccgcgcaaa ggtcgccgga ttgcaggaga cggctgcgat gtgggccgga ttcagccgga
    11581 ggatgccctg aacggtcttc gcgccggccc cggtgcgggg aggatcgaag acaactagtg
    11641 agacccggcc atgtcgggat tgatcctgtg tcacccaatg ctcgaccggc atggacatga
    11701 tctttgtttt cgtcagccct gccgcttcga ggttgtcgtt cgcgaacttc acggcgggct
    11761 tgaagctctc gaccccgatc acgcgcttgt acctgtgagc gagcggcacc gtgaagagcc
    11821 caaccccgca gtagagatcg atcgcgatac ctggctgctg ccgggcgata tcggcgatct
    11881 tcgtgacgtc ggcaaccagt ttctccgtca ccgggatatt tgcctggaag aagcactccg
    11941 ccgagaagtg gtaggactcg ccgccaatcg tgcgctggag aatcgccgaa cgtgtcgtgg
    12001 tcgtcggctc cagcgcgtat cctgtttccg tcgagacggc ccgatactcc ttcgcgttct
    12061 ccggcaccag tccagcactt acgtcatctc gcaacgtcgt caacagggta ttgagcccct
    12121 ccgtcgagac cgggtcaacc tcgacatcga cgattctccg tgtacctccc gcgtagtatc
    12181 cgaccacctt gttcgggacg ttcacctgcc actcagcgcg cgagcggtat ccccactggt
    12241 tttccgatgg ccggatcggg atctccggga tctcatcgag tttcgcgata cgccgcaggc
    12301 tgtcttggat gatgctgacc ttcgtcgcga gctggtccac atagctcagg tgcatcaggt
    12361 cgcagccgac gcagctcccg gcatggggat gatccggctg gatgcgcatg ggagacggcg
    12421 taatgatctc atcgatcacg gcgtggaccg tgctaccctg ctgccgtgta acacgggcgc
    12481 ggacgacatc gcccggcgcg gtgcgggtga cgaagatggt cttgccatcg acaaagccga
    12541 tgcctttgcc gtcaccgaca atgcggtcga tggtgacttc gatcgtggcc ggactttcga
    12601 ctgtactgct catggctgga ctttctgc
//
LOCUS       DASYWM010000118         9681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_642464, whole genome shotgun sequence.
ACCESSION   DASYWM010000118 DASYWM010000000
VERSION     DASYWM010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642464"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1186)
                     /locus_tag="VGR29_04890"
     CDS             complement(20..1186)
                     /locus_tag="VGR29_04890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2072961.1"
                     /translation="MRILQVLYYYEPYTSGLTVYAARLSRELAARGHAVSVLASRHDS
                     SLPSTEVTAEGVHIRRIPVAMSLDRAVIVPTLIPTAAVLMRTADVIHLHLPMAETAAL
                     VALGRVMGKRVIVTHHADLVLEGSRVSRVAATIARWSGIAGGKISSAFVTNTASRARV
                     SPVARYMSRNLSIVAPPIIVRAVEPGAGDTFRRDFGLGSGPVIGYVGRYASEKGIDVL
                     LQTIPAIREKLPDTLVALAGPRRDARDGSLLRGPWDRWLAKYAAAIRQLDYLSDDDLA
                     RFYAACDVLVLPSTDWTESFGMVQIEAMLRGTPVVASNLPGVADPIALTGYGELVRTG
                     DVDDLACSIIKVLTEPERFRPDPGRVAARYSLAATVDAYERLYRGEDAPGDPLP"
     gene            complement(1233..2270)
                     /locus_tag="VGR29_04895"
     CDS             complement(1233..2270)
                     /locus_tag="VGR29_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEV2072962.1"
                     /translation="MTFDSLATRAKSLAPGILLAVAVTVVALIIARIEEEILGHAILE
                     PLVLALILGLALRAMWAPPSFLEPGIRFAGKQLLEFAIVVLGLTLDLGDIVDAGTEIL
                     ISVLLLVSMTLIVGVVIGRAMGLGTRLAVLVAVGNAICGNSAIAAVAPAIRARKEEVA
                     SAIALTAVLGVGVVLALPLLVPLANLTDERYGVIAGLSVYAVPQVLAATFTVSPQAGQ
                     IASLVKLTRVMLLGPVVALFAVIFREQNDTSASEGRRVQLGKFLPWFVVGFAICATLR
                     TAGLVAESLIPFATDSSRILTAVAMAGLGLGVDMRSVRETGSRVIGVVLILTLLLVCS
                     AFVVTALLGIG"
     gene            complement(2576..3355)
                     /locus_tag="VGR29_04900"
     CDS             complement(2576..3355)
                     /locus_tag="VGR29_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2072963.1"
                     /translation="MQNIGEMFSLEGKIAAVIGGTGVLGGEFCRALAGAGAAVAILGR
                     SEARAQALAEEMRAAGQTCFPVPLDATNKEQLVGAYESIENVLGDVDILVNASGVNST
                     TPFLEIEEAEWQQILDTNLRTTFLACQVFGEKMIDTGRGGSIINISSASSGPPLSKVF
                     VYGVSKAGLNNLTQYLAREFAPYGVRVNAIAPGFFPAEQNRKILSRDRVDAILGHTPM
                     KRLGNPSELAGTLIWLASEHASGFVTGAIVRVDGGFSAMTI"
     gene            complement(3410..4207)
                     /locus_tag="VGR29_04905"
     CDS             complement(3410..4207)
                     /locus_tag="VGR29_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV2072964.1"
                     /translation="MRFGICYSPDQSLALAKAGYDFVEWPMSRTVGEMDEEAYQSLRN
                     LAQDLPVAPEAWNVMLPRSIMVVGPDADHTGMKRYIETALSRAAELGGQVVVFGSGGS
                     RMVPDAWSPEEAARQFDDACRIVGDVAAQNGITIAIEPLRRAETNLVNSVSEAAVIVK
                     RVGHSSVQILSDLYHVAEEDEPLRDTGAAAAILAHVHIAAPYSRAMPHPGEADSMYQE
                     YFRILQRSGYNQRISIECSPRPTVDEAAEGLKYIKSLWEEVASQAPV"
     gene            complement(4345..5778)
                     /gene="uxaC"
                     /locus_tag="VGR29_04910"
     CDS             complement(4345..5778)
                     /gene="uxaC"
                     /locus_tag="VGR29_04910"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEV2072965.1"
                     /translation="MTDEWQLSPDRNFDPDPTQRSIARDLYSSVKDLPIISPHGHADP
                     RLLADPQAQLGSPAELFIIPDHYVFRMLYSQGHQLEDLGVSPRDATTLETDHRRIWQR
                     FAENFHLFRATPTGQWLRDELIEVFGIQEKLDGESAQRIYDELDAKLRQTSFSPRALF
                     ERFNIETLSTTDPATSALEHHQTLQEEGWGQKIRPTFRPDALLSVDNAGWQNSVEQFS
                     NVSGVSIQDYSSLIQALEAHRQFFKRMGATATDHGVFTPHTHRLSDDEANAIFQRALS
                     GKADATDTAEFSAHMLMEMARMSCEDGLVMQIHAGSFRNHNQGLYERFGPDTGSDIPV
                     GVDWTRGMRELLNAYGGDPRFRLIVFTLDESTYGRELAPLAGHYPALLLGAPWWFYDS
                     PNGMRRYLDTVIETAGIYNTAGFNDDTRAFASIPARHDMWRRVTCNWLAGQVATGIID
                     QEDASEMAADFAYHLAKRAYRLDAETA"
     gene            complement(5839..6666)
                     /locus_tag="VGR29_04915"
     CDS             complement(5839..6666)
                     /locus_tag="VGR29_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV2072966.1"
                     /translation="MWTLTGFADEISPELDEQVTTLAAEEMQYLEFRSAWGTNVLDLS
                     DEELNRAKTMLGAAGIGVSSIGSPIGKIGITDDFAPHLDRFGRALEVAHRLESPYIRI
                     FSFFIPEGEDPASHREAVLERLRRLVNEAEGTGITLLHENEKHIYGDVPTRCLDLLTT
                     INSPTLRAAWDPANFVQCGVRPHTDGYESLRPFIDYVHVKDARLADGEVVPPGKGDGE
                     VRETIRALKTSGFDGFFSLEPHLAHAGAFSGFSGPDLFRLAVQSFKTLLQEEHVAWR"
     gene            complement(6688..7503)
                     /locus_tag="VGR29_04920"
     CDS             complement(6688..7503)
                     /locus_tag="VGR29_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2072967.1"
                     /translation="MGRVNRLLFYLVLTFFALLAAFPFYYMIVGSFMDAVEIYSRTPN
                     LWPASFDFDAYERIFNNVPMARYFLNSAIVSGGTTVIAVLASSAAGYCFAHLDFIGKR
                     FWFALVLATLIVPFQARIIPLFVMFTNYNLNNTYAGIMIPGLISAFGVFMMSQFFQSV
                     PAELREAAILDGASELGVFFRIFLPLAKPGVATLSVFTFISSWDDLLWPMIIAPNPDM
                     RTIQVGLAFIRQTAMTPDQVMAAIVVVSIPVIIAFLLGQRYFVAGITSGAVKE"
     gene            complement(7518..8384)
                     /locus_tag="VGR29_04925"
     CDS             complement(7518..8384)
                     /locus_tag="VGR29_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2072968.1"
                     /translation="MAKSIRKSRAAYLLMLPFLLHFLFVVAYPMFYSLYLSLTEAGLN
                     TDPAFVGLENFTTLAQDTDFRAALGNTFYFAFFAVLGETVLPLVVALVMNQALHGRVI
                     FRTAFFLPVATSWVIVSLMWSVLFSRNGIVNVGLAWVGLSQQPFLGDGQQAMWIIIAM
                     SIWKNLGYYMVIYLAALQSVPQDLVDAASLDGAGPLRVIWNVTIPSIRPVIYFVLSIA
                     TIGSMQLFTQPFIMTNGGPLNATLSIVMLLYRKGFVELAFGYGSAIATVLLVLLILLS
                     LLNRRLGNLIAH"
     gene            complement(8539..>9681)
                     /locus_tag="VGR29_04930"
     CDS             complement(8539..>9681)
                     /locus_tag="VGR29_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2072969.1"
                     /translation="VAKVEVQRTPLELMATGSALELTEIDAEAVRARSDELLNTNTTT
                     IKGGIWGLPYEYDPFGYIYNADMYREAGLDPDNPPKTWEEFRAVNEALKAEFPNAWTI
                     CHPLKNLNKIQPLVWGAGGRYWNDTVPPTESDVTNQGAIDAYNFFDEWAEKGWLNTEE
                     ISTDQSIQHMVSRQCAAINLSASAVLLYAANAPDTDFRVAAYPTKDESFEPANFAGGS
                     ALVIPSTAEHPEEALDFVLWLTSEETQRLKWGLEGDLGVAEQDIFDQDLPTNADAGEE
                     LAKDPAWEKAFATYDIPTRPAGGLSPVYSRAYEVLASMQERIALTDVDVEAELQAAEE
                     AVQALIDGNIAEMPELYEAVDNATPAATPDDIDDTLVDDATPVSTP"
BASE COUNT         1996 a         2788 c         2988 g         1909 t
ORIGIN      
        1 cggagaggac ccagttgaat catgggagag gatcccccgg agcatcttca ccacggtaga
       61 gacgctcata ggcatccacc gttgccgcaa gtgagtaccg ggctgcaacc ctgcccggat
      121 ctggacggaa gcgctccggc tccgtgagca ccttgatgat cgaacatgcg aggtcgtcca
      181 catcgcctgt tcggaccagc tcgccataac cggtcagggc gattgggtcg gcgacgccag
      241 ggagattgct ggcaacgacc ggtgtgccgc gcagcatggc ctcgatctgg accattccga
      301 acgattcggt ccagtcggtg ctcggcagca ccagcacatc gcaggcagcg tagaagcgtg
      361 ccaggtcgtc atccgagaga tagtcaagtt gtcggatggc ggcagcgtac tttgccagcc
      421 agcggtccca cggcccacgg agcaggctgc catcccgtgc atcccggcga ggaccggcca
      481 gcgcgacaag cgtatccgga agcttttcgc ggatcgcagg tatggtctgc agcagcacgt
      541 cgataccctt ctcggaggcg tatcggccga catagccgat gacgggtccg ctgccgagac
      601 cgaaatcgcg ccggaaggta tctccggcac ctggttccac tgcccggacg ataatcggtg
      661 gtgcaacgat cgacaaattc ctgctcatgt acctggcaac cggggagact cgcgcacggc
      721 tcgcggtgtt tgtgacaaag gcgctactga tcttgccgcc agcgataccg ctccaccgcg
      781 cgattgttgc ggccacgcgg ctaacccggc ttccctccag cacgaggtcg gcatggtggg
      841 tcacgatcac ccgctttccc atcacgcggc ccaaggcgac aagagccgcc gtctcggcca
      901 tggggagatg caggtggatg acatcggccg tgcgcatcaa taccgccgcc gttgggatca
      961 gggttggcac gatcaccgcg cgatccagcg acatcgcgac cggtatacgg cggatgtgga
     1021 caccttcggc agtcacctct gtgctgggca gcgatgagtc gtgtcgtgag gcgagaacgg
     1081 agacagcatg cccgcgtgcc gcaagctccc ttgagaggcg ggcggcatag acggtcaagc
     1141 cgcttgtgta cggctcatag taatagagaa cttgcagaat cctcacggcg ctccccggca
     1201 tcatgcgttt ggcgatatgc cccggatgaa ggttagccga tccccagcag cgcggtcacc
     1261 acaaaggcgg agcaaacgag aagcagggtg agaatgagca cgaccccgat gaccctggaa
     1321 ccggtttcgc ggaccgagcg catgtccacg ccaaggccga gtcctgccat cgcgaccgct
     1381 gtcaggatgc ggctggaatc ggtcgcgaag ggaatcaggg attccgcgac gaggccggcc
     1441 gtgcggaggg tggcgcagat ggcgaagccg acgacgaacc agggcaagaa cttcccgagc
     1501 tggacgcggc gtccttcgct ggcggaagtg tcattctgct cgcggaagat cacggcgaag
     1561 agcgcgacga ccggcccaag gagcatcacc cgcgtaagct tcacgaggct ggcgatctgg
     1621 ccagcttgtg ggctgaccgt gaaggttgcc gcaaggacct gtggaacggc atagacggag
     1681 agaccggcga tcacgccgta ccgctcatcg gtcagattcg cgagaggaac caggagcggt
     1741 agggccagga cgacaccgac acccagcact gcggtgagcg caatcgcact ggcgacttct
     1801 tccttcctgg cacgaattgc tggcgcaacg gccgcgatcg cggagttgcc acagatcgcg
     1861 ttgccaacgg caacgagcac ggcaagacgg gttccaagac ccatcgccct gccgatgacg
     1921 acgccaacga tgagggtcat cgacacgagc aggagaacgg agatgagaat ctcggtccct
     1981 gcatcgacaa tatcgccgag atcgagtgtt agcccgagca ccacaatcgc gaattcgagc
     2041 aactgcttgc cggcaaaccg gataccgggt tcgagaaagg aaggcggcgc ccacattgcg
     2101 cggagggcca gaccgaggat cagcgccagt accaacggct caagaatggc gtgcccgagt
     2161 atctcttcct caatcctggc gatgatgaga gcaacgacgg taacagcgac ggcgagcagg
     2221 ataccgggcg cgagggattt ggcacgagtg gcaagggagt cgaaggtcac gattcaggaa
     2281 atccagggtg cggtgatctg aagcgctccg gtcgagacag ccgccacagt gtaacgccaa
     2341 cgtggctaat cgtgaacact gcccacgggg tgtgtcaagg tgaggactcg aagcggatgt
     2401 gcggagttca atgggaggac gcggtgtggt cagccaggat tgccctacgc ctcccgcgtg
     2461 tgctggctcc gtgttcctta gccgtggtat gcctcgtgat gccttggccc tgaccagacg
     2521 acgatgggtt aggagtgacg aggataggcc cgagcctaga gatctgtacg cgccgtcaga
     2581 tcgtcatggc gctgaatcca ccgtcgactc ggacaatggc tccggtaaca aaaccagagg
     2641 catgctcaga cgccagccag atgagcgtac cggcgagttc cgaaggatta ccaagccgtt
     2701 tcatgggtgt atgtccgagt atcgcgtcca cgcggtcccg ggaaaggatc tttcgattct
     2761 gctcggcggg gaagaatccc ggagcaattg cattcacgcg aacgccgtag ggagcaaact
     2821 ctcgcgccag gtactgggtc agattgttta gccctgcctt gctcacgccg tatacgaaga
     2881 ccttcgataa cgggggccct gatgatgctg aggaaatgtt gatgatgctc cccccgcggc
     2941 cagtatcaat catcttttcc ccgaaaacct gacatgcgag aaatgtcgtc ctgaggtttg
     3001 tgtcgagtat ctgttgccac tctgcttctt ctatctcaag gaacggcgtc gtactgttca
     3061 cccccgatgc attgacaagg atatctacgt cgccaagtac gttttctatg gactcgtagg
     3121 ctccgactag ctgctcctta ttggtggcgt cgagtggaac ggggaagcat gtttgccctg
     3181 cggctcgcat ttcctccgca agtgcttgag ccctcgcttc tgagcggcca aggattgcca
     3241 ccgctgcccc tgctccggcc agagcacggc agaactcgcc gcccagcacg ccggtccccc
     3301 cgattaccgc agcaatcttt ccctccagcg aaaacatctc accgatattt tgcatcgccc
     3361 ccgccttctc ttctatgtat tcattcaagg caacatgccg gtggcagagt cacaccggcg
     3421 cttgcgaagc gacctcttcc cataggcttt taatgtactt aagtccctcg gctgcctcat
     3481 cgaccgtggg tcggggggag cattcgatgc tgatccgctg gttgtacccc gatcgctgca
     3541 gtattctgaa gtactcctga tacatggagt ctgcctctcc ggggtggggc attgcgcgac
     3601 tgtaaggcgc agcaatgtga acgtgggcga ggatagccgc ggctgctccc gtatccctga
     3661 gaggttcatc ttcctctgcg acgtggtaca ggtcggatag tatctggacg gaggaatgtc
     3721 cgacgcgctt tacgattacg gccgcctcgg aaacgctgtt gacgaggttc gtttcggcgc
     3781 ggcggagggg ctcaatcgca atcgtgattc cgttctgcgc ggccacgtca ccgacaattc
     3841 tgcaggcatc gtcaaattgc cgcgccgcct cctcgggaga ccacgcatca ggaaccatgc
     3901 gtgagccgcc gctcccgaag actaccacct ggcctccaag ttcggcggct cgggacaagg
     3961 ctgtttcgat atagcgtttc atcccggtgt gatcggcatc ggggccaacg accatgatcg
     4021 agcgcggcag catcacgttc catgcttcgg gggcaactgg tagatcctga gccaggttgc
     4081 gaagggattg atacgcttcc tcgtccatct cgccgacggt tcggctcatc ggccactcca
     4141 cgaagtcgta tccggctttg gcgagggcaa gactctgatc cggtgagtag cagattccga
     4201 aacgcatatc gttgctcctt gtgcccgtgg cgctcaacgc ctggttccat ggtcagcagt
     4261 ggctggatca agagggagag ggtgacctca tcatgccagg cgacgcaata tcgcgaaagc
     4321 gccgagcggt aaatggggtt ccccttaagc agtttctgca tccagccggt atgcacgctt
     4381 cgcaaggtga tacgcgaagt cagcagccat ctcgctcgcg tcttcctgat caatgattcc
     4441 agtcgctacc tgacctgcca gccaattgca ggttacgcgg cgccacatat cgtgacgggc
     4501 gggaatgctt gcaaaagcac gggtgtcatc attgaagccg gcagtgttgt agataccggc
     4561 ggtctcgatc acagtgtcca gataccgacg catcccattg gggctgtcat agaaccacca
     4621 gggagctccc aggaggagcg cggggtagtg accggcgaga ggggcgagct cacgaccgta
     4681 tgtgctctcg tcaagcgtaa acacgatcag gcggaagcgt ggatcaccac catacgcatt
     4741 gagcaattca cgcattccgc gagtccaatc gactcccacc ggaatgtccg acccagtatc
     4801 cggcccgaac ctttcgtaga ggccctggtt gtgattacgg aaactgccag cgtggatctg
     4861 cataacgaga ccatcctcgc agctcatccg tgccatttcc atgagcatgt gagctgaaaa
     4921 ctccgccgta tccgtcgcgt cggctttgcc cgagagagca cgctgaaaaa tggcgttagc
     4981 ttcgtcatcc gagagccggt gggtgtgtgg ggtaaagaca ccgtggtcgg ttgccgtagc
     5041 acccatacgc ttgaagaact gccggtgtgc ctcaagggct tgaataagtg aactgtagtc
     5101 ttggattgac acgccagata cgttcgagaa ttgctcaacg ctgttttgcc agcccgcgtt
     5161 gtcaacgctt agcagtgcat ccggacgaaa tgtcgggcgg atcttctgcc cccatccctc
     5221 ctcttggagc gtttggtgat gctcaagtgc ggaggttgcc ggatcggtgg tggaaagggt
     5281 ctcgatgttg aatcgctcga acagcgcgcg ggggctgaac gacgtttgcc gtagcttcgc
     5341 gtcgagttcg tcgtagattc gttgagcgct ctcaccatcc agcttctctt gtatcccgaa
     5401 cacctcgatg agctcgtctc gcagccactg acctgtcggg gtcgcacgaa agaggtggaa
     5461 gttctccgcg aagcgctgcc agatgcgccg gtggtcagtc tcaagggtgg ttgcatcacg
     5521 cggggaaacc ccaaggtcct ctagctggtg gccttggctg tagagcatcc ggaagacgta
     5581 gtgatcggga atgatgaaga gttccgcagg tgacccaagt tgcgcctggg ggtcggcaag
     5641 caatctcgga tcagcgtgtc cgtggggact gataatggga aggtccttga ccgaggagta
     5701 gaggtcacgg gcgatagacc gttgtgttgg gtcaggatcg aaatttctgt ccggcgatag
     5761 ctgccattca tctgtcacat tggtctccta cgcgcagtat gtctttcgcg gtaatgtcgc
     5821 caaacgcact tccccagatc accgccatgc gacatgctct tcctggagca gcgttttgaa
     5881 gctctgcacg gcgaggcgga aaagatccgg cccgctaaag cctgagaagg ctccggcgtg
     5941 ggccaggtgt ggctccagcg agaagaaccc gtcgaagccg gacgtcttga gggcgcgtat
     6001 ggtctcccgc acttcgccat caccttttcc ggggggcaca acttccccat cggcgagccg
     6061 cgcatccttt acgtgaacat agtcgatgaa tggtcggagg gattcgtagc cgtcggtgtg
     6121 agggcgtacg ccgcactgga cgaaattggc gggatcccat gccgcgcgca gggtgggcga
     6181 gttaatggtt gtgaggaggt ccaggcaacg ggtgggtacg tcgccgtaga tgtgcttttc
     6241 gttctcgtgg agtaacgtga tcccagtgcc ttcggcctcg ttaacaagcc tccgcaggcg
     6301 ctcgaggacc gcttccctat gtgacgccgg gtcctctccc tcggggatga agaacgaaaa
     6361 aatgcgaatg tatggcgact caagacgatg cgcgacctcg agcgcgcggc cgaaacggtc
     6421 gaggtgggga gcgaaatcgt cggtgatgcc gatcttgccg attggggagc cgatggagga
     6481 gacgccgatg ccggccgcgc cgagcattgt cttcgcgcga ttgagctctt catcactcag
     6541 gtcaagcacg tttgtgcccc aggcgctccg gaactcgagg tactgcatct cctctgccgc
     6601 gagggtcgtg acctgctcgt cgagttccgg tgagatctca tcggcgaagc ctgtcagtgt
     6661 ccacatgtgc gggttgctcc ttccatgcta ttccttcaca gcacctgacg taattccagc
     6721 cacaaagtat cgttggccga gcagaaacgc aataatgacc gggatagaga cgacgacgat
     6781 cgctgccata acctggtccg gagtcatcgc ggtctgccgg atgaacgcga gtccaacctg
     6841 gatggttcgc atgtccgggt taggagcaat gatcatgggc cacagcagat catcccagct
     6901 actgatgaaa gtaaataccg acaacgtcgc gacacccggc ttggcaagtg gcaggaagat
     6961 gcggaagaac acgccgagct cggacgctcc atcaaggatg gcggcctcgc gaagctcggc
     7021 ggggacggac tgaaagaact ggctcatcat gaagacacca aacgcgctga tcagcccggg
     7081 tatcatgatg ccggcatagg tgttgttcag gttgtaattg gtaaacatca cgaagagcgg
     7141 gatgatacgc gcctggaacg gcacgatgag cgttgccaat acgagcgcaa accagaaccg
     7201 cttgccgatg aaatcgagat gggcgaagca gtagcccgcc gcactggatg cgagtacggc
     7261 gatcacggtg gtgccgcccg agacgatggc gctgttgagg aagtatcggg ccatcggcac
     7321 gttgttgaag atgcgttcgt aggcgtcgaa atcgaatgat gcaggccata gattcggcgt
     7381 tcgggagtaa atttccaccg catccatgaa cgatccgacg atcatgtagt aaaaggggaa
     7441 tgccgcgaga agcgcaaaga aagtcagcac gagatagaag agcaatcgat tcactctacc
     7501 catggatcgg tgcctttcta gtgagcgatc agattgccca ggcggcggtt gagtagcgac
     7561 agcaggatca gaagcaccag caggacggtc gcgatggctg atccgtaacc gaaggccagt
     7621 tccacaaagc ccttccggta gagcaacatc acgattgaga gtgttgcgtt gagaggccct
     7681 ccgttggtca tgatgaaggg ctgcgtgaac aactgcattg agccgatggt ggcgatgctc
     7741 agcacaaagt agataacggg gcggatactc gggattgtca cattccagat cacgcggagt
     7801 ggcccagccc cgtcaagcga tgccgcgtcg acgaggtcct gggggacgct ttggagtgcc
     7861 gccagataga tcaccatgta gtatccaaga ttcttccaga tactcatcgc gatgatgatc
     7921 cacatcgctt gttgaccgtc gcccaggaac ggctgctgcg acaagccgac ccacgcaagg
     7981 ccgacgttga cgatcccatt gcgtgagaac aggaccgacc acatcagcga gacgataacc
     8041 cacgacgtcg ctacgggcag gaagaacgcc gtgcggaaga tgactcgtcc gtgcagagcc
     8101 tggttcatga cgagtgccac gacaagtggc aggacggttt ctccaagcac cgcgaagaag
     8161 gcgaagtaga aggtgttgcc cagggcggcc ctgaagtccg tgtcttgagc gagagtggtg
     8221 aagttttcca gacccacaaa tgccgggtcc gtgttgagcc cggcttcggt gagactgaga
     8281 tagagcgaat agaacatggg ataggcgact acgaagagga aatgcaagag aaagggaagc
     8341 ataagcagat aggccgcacg gctcttccgt atggacttcg ccatcctttt cctgcgcgtt
     8401 gccgttgtta ttgggcgttg ctctacaagc tctgcaatcg ccatccctgc cccctccctc
     8461 atcaagcatg tcgcgtgact ggcaattggt tcggtaagga caggccctgg agcagccgct
     8521 ccagggcccg ttgcacgcct atggtgtcga gactggggtc gcgtcatcca ccaacgtgtc
     8581 gtcgatgtca tctggtgtcg cggccggggt tgcgttgtcc accgcttcgt agagctctgg
     8641 catctccgcg atgttgccgt cgatgagcgc ctggaccgcc tcctccgcgg cctggagctc
     8701 cgcctcgacg tccacatcgg tcaaggcgat ccgctcttgc atcgaggcaa gcacctcgta
     8761 cgctcgggag tacactgggg acagtccccc agccggccgg gtcgggatgt cataggtcgc
     8821 aaatgctttc tcccaagcgg ggtctttggc caactcttcc ccggcgtcag cattcgtagg
     8881 caggtcctgg tcgaagatgt cctgctcggc gacgcccagg tcaccttcca ggccccactt
     8941 gaggcgttgt gtttcttcgc ttgtcagcca caggacgaag tccagcgcct cctccgggtg
     9001 ctcggcggtt gacgggatga ccagggcact cccgcccgcg aaattcgccg gctcgaagct
     9061 ctcgtccttg gtcggatatg ccgccacccg gaagtcggtg tccggggcat tggctgcata
     9121 caggagcacg gcggatgcgc ttagattgat ggcggcgcat tggcgcgaca ccatgtgctg
     9181 gatcgactga tccgtgctta tctcttcagt gttcagccat cccttctccg cccattcgtc
     9241 aaagaagttg taagcatcaa tggcgccctg attcgtcacg tcggactcgg tcgggggaac
     9301 cgtatcgttc cagtaccgcc cgcccgcgcc ccagaccagg ggctggatct tgttgagatt
     9361 cttgaggggg tggcagatcg tccatgcgtt ggggaactcg gccttgaggg cttcgttgac
     9421 ggcccggaac tcctcccatg tcttcggcgg gttatccgga tcgagccctg cctcccggta
     9481 catgtcagca ttgtagatgt agccaaaggg gtcatactcg tatggaagtc cccagatgcc
     9541 acccttgatt gtggtcgtat tggtgttcaa cagttcgtct gatcgggccc ggacggcctc
     9601 ggcgtctatc tcggtgagtt ccagcgcgga gcctgttgcc atcagttcaa ggggcgtccg
     9661 ctgcacttcc acttttgcga c
//
LOCUS       DASYWM010000119        15972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_530063, whole genome shotgun sequence.
ACCESSION   DASYWM010000119 DASYWM010000000
VERSION     DASYWM010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15972
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530063"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VGR29_04935"
     CDS             <1..343
                     /locus_tag="VGR29_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072970.1"
                     /translation="AWQCQDLDALRALDQELDQMLPGWSANHLAHRLAWARRECERLN
                     VQAEALVDRIHQLRTSETFPLWFNSTLGSTVIAQRANALRRELEREEARLEDAKLAFK
                     QALAYYAAAVA"
     regulatory      424..541
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            702..1907
                     /locus_tag="VGR29_04940"
     CDS             702..1907
                     /locus_tag="VGR29_04940"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEV2072971.1"
                     /translation="MTNRPETSIHTTTHDPMQRHLHIVPSYAPSGDGVMRLAWFGPMD
                     LELGGHLPDVTVAYRTWGKLNDAGDNAVIVLHALTGDSRAADDGGWWEPVIGPGSPID
                     TDQSFVMCANVLGGCQGSTGPASHDPSGRPWGTRFPLITIGDIVNVQRRLVDQLGIKK
                     LVAIGGSIGGLQALEWATRHAELVRGAGVIAASGALSALGIAIHGEIGRRAIMADPNW
                     RDGDYVEHRVFPEQGLAIARMAAMTTYHSRESLGSRFGRNPASRPTLYPSFGPTFDVE
                     GYLHYHGRALAQRFDANSYLYLTRAMDLYDVGRDGGDDYWFDQITAPLLLVGISSDWL
                     FPSEDICLLADRIRDRGKDVTFCEIESPNGHDAFLKDWNELNPIIDHFMDYVGAPDTL
                     TPLTDGASG"
     gene            2064..3353
                     /locus_tag="VGR29_04945"
     CDS             2064..3353
                     /locus_tag="VGR29_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEV2072972.1"
                     /translation="MVDEKQLGFETESLHAGQEPDPTTGSRAVPIYQTTSYVFNSTEH
                     AANLFGLREFGNIYTRIMNPTNDVLEKRIAALEGGVAAVTVSSGQAATTLTLLTLAKA
                     GDEIVSTNSLYGGTYNLFHYTLPRMGITVKFVEGKPDAIRGAITNKTKAVYSESIGNP
                     DLLTLDIQAVADVAHEHGVPLVIDNTMPTPYLIRPFDHGADIVVHSLTKFIGGHGTSI
                     GGIVVDSGNFDWTASPRFKEDFVDPDPSYHGLSFSGAFGNLAFALKVRTQGLRDLGAA
                     LSPFNAWQFLQGTETLALRMERHSQNAMQVAQFLNEHPKVDWVNYPGLPSHTNYEIAS
                     KYWRDGLFGAILGFGVKGGAEEGRKVVESVEIFSHLANIGDAKSLIIHPATTTHSQLT
                     DEEQVATGVSPDYLRLSIGLESVGDLIADLEQALGKI"
     gene            complement(3636..4160)
                     /locus_tag="VGR29_04950"
     CDS             complement(3636..4160)
                     /locus_tag="VGR29_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2072973.1"
                     /translation="MAVLMRAGRPEDVPLLAPIEQAAGRLFLEVGMPEIAGDDPVPEE
                     MLLQGVDAGLLWVAVDEGESLAPAGYLLAKNLDESRHIEQLTVHPRYARQRIGSRLID
                     ACGEGAMASGFRSLTLTTFRDVPWNAPYYRRIGFVEVPDTELSPALREIVQHEHDAGL
                     DRWPRVVMRRTLTH"
     gene            complement(4160..5365)
                     /locus_tag="VGR29_04955"
     CDS             complement(4160..5365)
                     /locus_tag="VGR29_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEV2072974.1"
                     /translation="MTHLLARFARTLPGLVLLLEVVLAPVAIAAQDMPTAFDPATFDV
                     GLEPIADGFVQPLFVTDSNDDTGRLFVVEKGGTIRIIVGNEVIQTPFLDITDRVGDDA
                     YEQGLLGLAFAPNYAESGLFYVNYTDRAGNSVVSRFSVSGDPNLADAGSEELLLRQQQ
                     PYANHNGGMMMFGPDNYLYIGFGDGGSGGDPQGNAQRLDTWLGKILRIEVDPRYVPEG
                     QPYGIPEDNPFAGEEGALPEIWAHGLRNPWRFSFDRETGDFWVGDVGQNAIEEINYVP
                     INAVPGQNFGWNITEGTVCYLEPGCDTSGFLQPVLEYTHDVGGCSVTGGYVYRGEEMP
                     DLAGTYLFADFCSGLLWGGGQDASGAWAMSAPIETGLSISSFGEDDAGNLYVTDLGDG
                     VVYLVAPPL"
     gene            complement(5470..5868)
                     /locus_tag="VGR29_04960"
     CDS             complement(5470..5868)
                     /locus_tag="VGR29_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072975.1"
                     /translation="MNERDVSGNVSTWSVILGVLLGVLLFLGSAYSGSSIAGLGVVLL
                     VLAYVIVLSAIGRVNVFQVLWGGMADESDPETNQRVILLATVVIALVAVGVLVYDLAS
                     ESDWGWFSWLGAAIAIGYLAVVGWHHSRSQ"
     gene            complement(6194..7195)
                     /locus_tag="VGR29_04965"
     CDS             complement(6194..7195)
                     /locus_tag="VGR29_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2072976.1"
                     /translation="MLLCVYIGLGSVAAAAPGSFQWSTLDQTLGSLSTSSNLLVAEFR
                     GSTCQTIHGRNQDQRLAIASTFKIYVLGELARQIQLGSAAWDEGTEVTDTLRSMPSGD
                     YAFVPAGTRVTLRELAQAMIWQSDNTATDHLIARLGRENVQRAFQAYGHGDPSLNAPL
                     LLTKEMFGIKMSQSPEWMSAWSAAPEDVQLAMLEETIDPMQINPSGGWGMWNGPTAID
                     GIEWFASADDLCRATAALWSMGAQPGLEPVRDILTGNRYGVTDTGAWPRAGYKGGYEA
                     GVVNMTFVLERNDGRVFFVSAGYNDPVRVVDTGTARTYLEPVFGCLKTYTSDYACGG"
     gene            7204..8043
                     /gene="lepB"
                     /locus_tag="VGR29_04970"
     CDS             7204..8043
                     /gene="lepB"
                     /locus_tag="VGR29_04970"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV2072977.1"
                     /translation="MLVGNGATAWTTPNLGQICADRLSMVSVQSMLLLWIPASKMCHH
                     VTMTTGHRVGTNANDIRSQHEQGMDSFPLVLPPKGPGPRQRFRLLRELKPLLLIVLVV
                     FAGVRMFFQPYEVDGASMSPTIANGNRLFVNRSAYAHVDLPALGDIYPFGDPRRGDII
                     VLESDRTSRSAPYIKRIIGLPGETITYTEGIVIVDGEPLVEDYINGAVTGCYVPTHCS
                     ITIPQGHVYVLGDNRRDSEDSRFFGPVPMEDIVGKAFFSNWPYEDIGPIQHPDYGEIS
                     AEL"
     gene            complement(8126..9271)
                     /locus_tag="VGR29_04975"
                     /pseudo
     CDS             complement(8126..9271)
                     /locus_tag="VGR29_04975"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
     gene            complement(9329..10000)
                     /locus_tag="VGR29_04980"
     CDS             complement(9329..10000)
                     /locus_tag="VGR29_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEV2072978.1"
                     /translation="MRRSGEGFAGAAKKTDRRSFLRAAGIGGLTAAVLPSRDRVAGAQ
                     DALSDPADADSRMGAPGGISVFPGHGTMTASPATEISFRGVSIVSLGPVRAAGSKSGG
                     HSGILSEHADGRGVSFLPDAAFEPGESVTVSAGVALRQSAEGSVVFTVARPATKVAPA
                     AYREVNKPAGPTQTFRSRPDLRPPVIEVTTEAVDTAPGHIFVAPRIPDGQSGAMILDQ
                     NGELV"
     gene            complement(10130..10456)
                     /gene="sugE"
                     /locus_tag="VGR29_04985"
     CDS             complement(10130..10456)
                     /gene="sugE"
                     /locus_tag="VGR29_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEV2072979.1"
                     /translation="MNWVVLIVAGFVEMLMALSLKESHGFSRWTPSILFVVFAVLSFA
                     MLSYSLKTLPVGTAYAVWTGIGAAGTAVLGIALLGEPRDAARLVSIVLIIAGVVGLRL
                     AGGGPS"
     gene            complement(10573..11997)
                     /gene="hemG"
                     /locus_tag="VGR29_04990"
     CDS             complement(10573..11997)
                     /gene="hemG"
                     /locus_tag="VGR29_04990"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEV2072980.1"
                     /translation="MQTVAVVGGGISGLAAAYRLVTSGQDVRVLVLEASDHLGGIVRT
                     EHVDGFTIDGGPDSFLLMKPRGIGLARELGLEDRLQWTNEANRGSFLYRKGALHPLPE
                     GLTGLIPTKLAPMVKTPLVSPRGKARMALDFVLPSRKGDGDESLRAFISRRLGPEVFD
                     NLIEPLMAGIYSGDGSQLSLAATFPQLRAAEKEHGGLIKGVLAGRKQQTMRAVGHQTR
                     QGFVSFRTGMHELPDALKTAIETHGGVIKTRRRVTRIDQMDDGRFVVAQDGENRAWSD
                     LFDKVILAAPVCVQAGLLQYLDAEAAHAMAEIPVASSAAVLLGYRETGEVIAPRDYGY
                     LVPRVEQRPIKAITWLSSKWEDRAPEGHFLVRAFIGRVGQQEVLQHSDDELVGLVRQE
                     LREVSAISGDPVVQRVYRLNEAMPQYTVGHLERVARLEAALERVPGLEIAGNMLRGVG
                     IPDIIAAGESAADRLLGRQDPVSS"
     gene            complement(11997..12965)
                     /gene="hemH"
                     /locus_tag="VGR29_04995"
     CDS             complement(11997..12965)
                     /gene="hemH"
                     /locus_tag="VGR29_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV2072981.1"
                     /translation="MASESTASAGEQATHHLLIMAYGGPDKIEDVRPYLLDVRHHRPT
                     SEEVFHEIEERYKLIGGRSPILELTRAQGDGIEQALNEQAPDGETWKVWDGMRHWYPY
                     ISETLSKMKEAGVERAVTLVMAPHYSRMSIGAYNKAVDDAASGIETVAIDRWNLLPGY
                     LDALEERINDALQKFPEDVRDNVPVIFTAHSLPERIREWNDPYESDLEATYVAMKDRF
                     PGHPAEWAFQSAAMTRDPWLGPDASEVIDRLHRDGVEHVLICPVGFVCEHVEILYDID
                     VEFAQQARGLGMQLERIQMVNDHPKMMAGLAQLVRDHARNADWVTA"
     gene            complement(13307..13939)
                     /locus_tag="VGR29_05000"
     CDS             complement(13307..13939)
                     /locus_tag="VGR29_05000"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEV2072982.1"
                     /translation="MANVLVGHRVVRTYQVGVGTAVSFEVAANQLIQIIDLAGHQVAG
                     FVAYGGEDRRERLSTSTTMTANASIVLKKGDKLYGTDRTALFEVMDDTVGRHDLITGP
                     LPVPETTTTATTVQPTSRDSLIAAANEAGLNGVDVSDPVNWFKHVIIKQRGELEVKES
                     FSERGDTVVLRALTDAVVLVSNSYHERRPGVTAGKTAVDKEGELIVRVYA"
     gene            14201..15352
                     /gene="chrA"
                     /locus_tag="VGR29_05005"
     CDS             14201..15352
                     /gene="chrA"
                     /locus_tag="VGR29_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEV2072983.1"
                     /translation="MAESALRSVVLVAFKLGCIAFGGPAAHIAMLRQEVVERRRWVEE
                     QRFLDLMGITNLIPGPNSTELVIHIGRERAGIKGLIGAGAAFILPAASITLFFAWLYM
                     SYGTTTQGEWLLRGIKPVVLAVVMQAIWNLGKTAFKTLPLTIVGALVFVLYLLGGNEI
                     LLLFGGALVAFLMQRILASGHRSSASSFVAPFLVPGAFSLVLADVATVPYSALRLFLT
                     FLKIGSVLYGSGYVLVAFLQNDFVERLGWLSEKQLLDAVAVGQFTPGPVFTTATFVGY
                     VVGGFPGAVLATLGIFLPAFIFVGIAHALIDRLMGLTWVRPLLDGINVAAVGLMAAVA
                     VILAQDAVVDIMTAVLFLAALALLIRYKVNSAVLVAAGALISLVREFLL"
     gene            15511..>15972
                     /locus_tag="VGR29_05010"
     CDS             15511..>15972
                     /locus_tag="VGR29_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2072984.1"
                     /translation="MERCSNCGAATRPGAKFCTSCGTRLNLDQESTGPTDSSWNSASS
                     TQETSVATPAIEDGDITTERDAVTPPPHWEETVQDPRPENGDEAPAAAWSSGSPWTAG
                     EAEPSAEAEAQNVRGSEDSDWSSKWHSEERVDEAVGTGSSTDSTSPEDNRWA"
BASE COUNT         3179 a         5133 c         4666 g         2994 t
ORIGIN      
        1 cgcctggcag tgccaggatc tcgacgcgtt gcgggctctc gatcaggaac tcgaccagat
       61 gctgcccggc tggtccgcga accacctcgc gcaccgcctt gcctgggccc gtcgcgaatg
      121 cgagcggctg aatgtgcaag cggaagcgct tgtcgaccgc atccatcaac ttcgcacgag
      181 cgagacattc ccgctctggt tcaactccac actcggcagt acagtcatcg cccaacgtgc
      241 gaacgccctt cggcgcgagc tcgagcgtga agaggcgcgg ctggaggatg caaaactcgc
      301 ctttaagcag gccctcgcct attacgctgc tgcagtcgcg tgaatgcgct ttcggacaca
      361 cgtgcgcaga cagcttgacg ccgctgcttc tacgccgcta ccattgcgca acaatcgaag
      421 actgccttat ccagagaggt ggagggaatc ggccctgtga agcccggcag cctgcgaagc
      481 atcctgaaga ggactgatgc gtaaggtgcc aactccggca gagagatctg cgagataagg
      541 gatcagcgat ctgcgcacgc actcgccccc ctgctctggc acgggggttt ttcgatcccc
      601 gccaccagcg ctccgtcctt cggcttaagc ccgcgggatt ctctctccga acctcatcgc
      661 ctctggacca tgagagactg aggagagaag gcggcacgtc catgacgaac cggccagaaa
      721 catctatcca taccaccacg cacgacccga tgcagagaca tctgcacatt gtgccgtcat
      781 atgctccctc gggcgatggc gtcatgcgcc tcgcctggtt cgggccgatg gatctcgagc
      841 tcggcgggca tctgccggat gtcaccgtcg cctaccgcac gtggggcaag ctcaatgacg
      901 ccggcgataa cgcggtcatc gttctccacg ccctgaccgg ggacagtcgc gccgccgacg
      961 atggtggctg gtgggagcca gtgattggtc ctggctcacc catcgacacg gaccagtcat
     1021 tcgtcatgtg cgccaatgtc ctcggtggct gccagggcag caccggaccc gcttcccacg
     1081 atccctcggg ccgaccttgg ggaacacgct ttccccttat cacgatcggc gacatcgtca
     1141 acgtacaacg caggctcgtc gatcagcttg gtatcaaaaa actcgttgcc atcggtggct
     1201 cgatcggggg tctgcaagca ctggagtggg ctacccggca tgcggagctt gttcgtggtg
     1261 ctggcgtgat cgcggccagc ggtgcgctga gcgctctcgg catcgccatc catggcgaga
     1321 tagggcgccg cgccatcatg gccgacccca attggcgcga cggagattac gtcgaacatc
     1381 gcgtcttccc ggaacagggt ctcgcgatcg cccgcatggc cgcgatgacg acctaccata
     1441 gccgtgaaag ccttggcagc cgattcggac gcaacccggc cagccgcccg acgctctatc
     1501 cgtcctttgg accaaccttc gatgtcgagg gttatctgca ctaccacggc cgggcgcttg
     1561 cccagcgctt cgacgcgaac agctatctct atctgacccg ggcaatggac ctctacgatg
     1621 tcggccggga cggtggtgat gattactggt tcgaccaaat cacagcaccc ctgctgcttg
     1681 tcggcatcag ctctgattgg ctgttcccct cggaagacat ctgtcttctt gctgaccgga
     1741 ttcgcgaccg gggcaaggac gtcaccttct gcgagatcga gtcccccaac gggcacgatg
     1801 ccttcctgaa ggattggaat gagctcaacc cgatcatcga ccacttcatg gactatgttg
     1861 gagcgccgga cacgttgacg ccactcaccg atggagcaag tggatgagat tacgggatct
     1921 ccttgcgacg tcgcgccacc gtgcgcgtga cgggttgatg caggtcatct actgacgcgg
     1981 gcaatcgcgg aagcatgagc cgacgcacct gatattgttg tattcaggaa acagccgttc
     2041 ttgaaaggga gaatatatct accatggttg atgaaaagca gcttggcttc gagaccgaat
     2101 cactgcacgc gggtcaggag cctgatccga ctaccggctc acgggccgtg ccgatctatc
     2161 agacaacctc gtacgtgttc aacagcaccg aacacgccgc caatctcttt ggactcaggg
     2221 agttcggaaa catctacacc cggatcatga atccgaccaa tgatgtcctc gagaagcgca
     2281 tcgccgcgct ggagggaggc gtcgcggcag tgaccgtctc ctcaggccag gccgccacga
     2341 cactcaccct cctgaccctt gccaaggcag gagacgagat cgtctcgacc aacagcctct
     2401 acggtggcac ctacaatctc ttccactaca cgctgccgcg catgggcatc accgtgaagt
     2461 tcgtggaagg gaagccggac gccatccggg gtgcgatcac gaacaagacc aaggccgttt
     2521 attcggagtc tatcggtaac ccggacttgc tgaccctcga cattcaggcc gtcgcagatg
     2581 ttgcccatga gcacggcgtg ccgctggtca tcgacaacac gatgccgacg ccgtatctga
     2641 tccgcccgtt cgaccacggc gctgacatcg tggtccactc gctcaccaag ttcattggcg
     2701 gacacggcac ctcgatcggc ggaatcgtcg tcgactccgg caacttcgat tggaccgcct
     2761 ctccacgctt caaggaagac ttcgtcgacc cggacccaag ctaccatggc ctgagcttct
     2821 ccggagcgtt cggtaacctg gccttcgccc tgaaggttcg cacccaaggc ctgcgtgacc
     2881 tcggcgcggc cctcagcccg ttcaacgcgt ggcagttcct gcaggggacc gagaccttgg
     2941 ccctccgcat ggagcggcac agccagaacg cgatgcaggt ggcccagttc ctgaatgagc
     3001 acccgaaggt ggattgggtg aactacccgg gcctgccaag ccacacgaac tacgagatcg
     3061 cctcgaagta ctggagggac ggcttgttcg gagccatcct cggcttcggc gtcaagggcg
     3121 gtgccgagga aggacgcaag gtagtcgaga gtgtcgagat cttctcgcat ctcgccaata
     3181 tcggcgacgc caagtcgctg atcattcacc ctgcaacgac cacccattcc cagctgacgg
     3241 acgaggagca ggtcgcgacc ggtgtctcgc cagattacct gcgcctctcg atcggacttg
     3301 aatcggtcgg cgatctcatt gccgacctag agcaggcgct tggcaagatc tagcgatcat
     3361 tcccgcgccg attgaaccaa ggggagcccc aggccacaac acccggggct ccgtatggat
     3421 gagagccgga tttccgcttg tcgcggaacc ttttgggcgc cgctggcgtt caaatcagat
     3481 agaagacggc acacgtaacc gcatcaccag ttgatgccga tgggaagcaa gagacccttc
     3541 caacgcaggc tgtcttcctt ccccacaagg caaatgcccc ggattccgtc caccgacagg
     3601 atccggggca ttctgctgat ccctacctgg agaactcaat gtgtcaacgt ccgccgcatg
     3661 acgacccgtg gccaacggtc gagccccgcg tcgtgctcat gctgcacgat ctcccggaga
     3721 gccggcgaca actctgtatc aggcacctct acaaatccga tgcgtcggta gtagggagca
     3781 ttccagggaa catcacgaaa tgtggtcagg gtcagcgagc ggaacccgga tgccatcgcc
     3841 ccctccccac aggcatcaat cagccgggaa ccgatacgct ggcgggcata acggggatgt
     3901 actgtcaact gctcgatatg acgactctcg tccaggttct tcgcgaggag gtagccagcc
     3961 ggtgccaggc tctcgccctc gtcgaccgcg acccacagca gcccggcatc cactccctgc
     4021 aagagcattt cctcggggac gggatcgtca ccggcaatct ccggcatgcc cacctccagg
     4081 aagagccgac cggccgcttg ctcaattgga gccagcagcg gcacatcctc cggcctcccg
     4141 gcacgcatca ggacggccat cagagtggtg gcgccacgag atagaccaca ccgtcgccaa
     4201 ggtcggtaac atagagattc cctgcgtcat cctcgccaaa ggatgagatg gacagccctg
     4261 tctcgatcgg cgcggacatc gcccaggcac cgctggcatc ctgcccgccg ccccagagca
     4321 gtccgctaca gaagtctgcg aacagatagg ttcccgccag atctggcatc tcctcaccgc
     4381 ggtagacata cccgcccgtg accgagcagc cacccacgtc atgggtgtat tccagcaccg
     4441 gctgtaggaa tccgctcgtg tcgcacccag gctccaggta acacaccgtg ccttcggtga
     4501 tattccagcc aaaattctgc cccgggacgg cattgatcgg cacgtagttg atctcctcga
     4561 tcgcgttctg ccccacgtcg ccaacccaga agtcgccggt ctcccgatca aagctgaacc
     4621 gccacgggtt gcgcagaccg tgggcccaga tctccggcag tgcaccctcc tcccccgcga
     4681 agggattgtc ctcgggaatg ccataaggtt ggccttcggg gacgtaccga ggatcaacct
     4741 cgatccgcag gatcttgccg agccatgtat cgagacgctg tgcgttgcct tggggatcac
     4801 cacccgagcc gccgtcgcca aagccgatat agagatagtt gtcgggcccg aacatcatca
     4861 tgccgccgtt gtggttcgca tagggttgct gctgtcttag cagcagctct tcgcttccgg
     4921 cgtcggcgag attgggatcg ccacttaccg aaaagcgcga aacaacggag ttgcccgcac
     4981 gatcggtgta gttgacgtag aaaagcccgg actccgcgta gttcggggca aacgccaacc
     5041 ccagcagtcc ctgctcgtag gcgtcgtcgc ctacccggtc ggtgatgtcg aggaagggcg
     5101 tctggatcac ctcgttaccc acgataatgc ggatcgttcc tcccttctcg accacgaaga
     5161 gccgaccggt atcatcgttc gagtctgtca caaagagcgg ctgcacgaat ccatcggcga
     5221 tgggctccag gccaacgtcg aaagtcgccg gatcgaaggc tgtgggcatg tcctgcgcgg
     5281 cgatagcgac cggggccaga accacctcga gaagcaacac caaacctggc agcgtgcgag
     5341 cgaatcgggc tagtagatgt gtcacgatga aaccccttcg catttcttgc gcacgccgtt
     5401 ggtgcaattc atccgatctc aatgcgtcgt tgccgttgcc gctgtcactc ggagtcccta
     5461 gtccggttct cattggcttc tggaatgatg ccacccgaca acagcaagat atccaatcgc
     5521 gattgcagcg cccagccagg agaaccatcc ccagtcactc tctgacgcga ggtcgtagac
     5581 aaggacacca acggccacga gggcgatcac gaccgtcgcc agcaagatca cacgctggtt
     5641 ggtttcggga tcgctctcgt cggccatccc tccccacagc acctgaaaga cgttcacgcg
     5701 cccgatcgcc gagagcacga ttacataggc aagcaccaac agcacgacgc ccaatcccgc
     5761 aatcgagctc cctgagtagg ccgagccgag aaagagcagc actcccagca gcacgccgag
     5821 aatgacagac caggtcgaca cattgcccga tacatcgcgt tcattcatcg atccggaata
     5881 cctcctcgac gattctgcga agaaatcggg caacctttcc ccaggatggg agaaggtaca
     5941 cactcgtgct aatgggtatc tggtccaaga accagggaca cggttacata gacgggcacc
     6001 ggattctgcc aacgctaacc gctacagcaa gtgtgcagga aatcgcctcc ataggcacca
     6061 ggcttgaggg agcaaggata tggcgccacg caggcaagtt cattgtgaga tggtgattgc
     6121 ctgtctgcaa cgacctctcg cgttgttagc cggggctcgt catcgcttgc agccaggctc
     6181 agccgcacaa cggtcagcca ccgcaggcat agtcgctggt ataggttttc aggcatccga
     6241 atacaggctc gaggtaggtg cgggcggttc cggtatctac cacgcgaacc gggtcgttgt
     6301 aaccagcgga gacgaaaaag acgcggccgt cattgcgctc aagcacgaat gtcatgttta
     6361 caacgcccgc ctcgtagccg cccttgtacc cggcacgggg ccatgcgccg gtatcggtga
     6421 caccatagcg attgcccgtc aggatatccc tcaccggctc cagacccggc tgcgcaccca
     6481 ttgaccagag cgccgccgtc gcccggcaga gatcatcggc cgaggcgaac cattcgatgc
     6541 catcgatcgc cgtggggcca ttccacatgc cccagccacc ggatgggttg atctgcatcg
     6601 ggtcgatcgt ctcttccaac attgccaact ggacgtcctc aggcgctgcc gaccaggcag
     6661 acatccattc cggggattgg ctcatcttga taccaaacat ctctttcgtc agaagcaagg
     6721 gagcgttcag cgatggatca ccatgtccgt aagcctggaa ggcgcgctgg acattctccc
     6781 tgccaagccg ggcgatcagg tgatcggtcg cggtgttatc actctgccag atcatcgcct
     6841 gagccagttc cctcagggtg accctcgtcc ctgctggcac aaacgcgtag tctcccgagg
     6901 gcatcgagcg gagcgtatcg gtcacctctg tcccctcatc ccacgctgcc gagccaagct
     6961 ggatctgccg tgcaagctcg cctagcacat agatcttgaa ggtggatgcg atggccagcc
     7021 gttgatcctg attacgaccg tggatcgtct ggcaggtgct gccgcggaac tcggcgacga
     7081 gcaagttgct cgacgtagac agcgaaccaa gcgtttgatc caatgtcgac cactgaaacg
     7141 aaccaggtgc cgccgcggca actgatccaa ggccgatata cacacacagg agcatgagag
     7201 cggttgctcg taggaaatgg cgcgacggca tggacgactc ccaacctggg gcaaatctgt
     7261 gcggaccgcc tgtcgatggt gtccgtacag tccatgctac ttctctggat acccgccagc
     7321 aagatgtgcc atcatgtcac gatgaccact ggccaccggg taggcaccaa cgccaacgac
     7381 atccgatcac agcatgagca gggcatggac agcttccccc tggtgctccc accgaaagga
     7441 ccggggccgc gccagcgatt ccgcctgcta cgcgagctga aaccactgct gctcatcgtg
     7501 ctggtcgtct tcgccggggt acggatgttt ttccaaccct atgaggtcga tggcgcgagc
     7561 atgtcgccga cgattgccaa tggcaatcgg ctcttcgtca atcgatcggc ctatgcccac
     7621 gtcgacttac cggcgctcgg cgacatctat ccatttggtg acccccggcg gggcgacatc
     7681 atcgtgcttg aatccgatcg cacaagccgc tctgcgccgt atatcaaacg gatcatcggc
     7741 ctgccggggg aaacgatcac ctacaccgag ggcatcgtga tcgtcgacgg cgagccgctg
     7801 gtggaggatt acatcaacgg cgcagttacc ggatgttacg ttccgacgca ctgctccatc
     7861 accatcccgc aaggacacgt ttatgtcctc ggcgacaacc ggagggacag tgaggactcg
     7921 cgattctttg gaccagtccc gatggaggat atcgtcggca aggccttctt cagcaattgg
     7981 ccctatgagg acattggacc catacagcat ccagactacg gcgagataag cgcggaatta
     8041 tgagatcgtg tgccggatgt cgcatcccga tacatgccgc actgtagggt gcattgtctc
     8101 tcccggggtc gagcagaagc cgcgcttact ttacctttgc ggcttccgat gtcccgagca
     8161 cattgcccat gttatcaagc gcctgcacgg caacatattg ctcgctgagc cgggtctcga
     8221 tcctcgtttc gaagccggta cgtggcaggg tagccggacc aagcgcacgc atgcgatcag
     8281 ggccggaacc ggaaagcacc ctccagcggg aaacctcggt tgcaccattc caactggcgt
     8341 agatcgcagc acggtcaccg gtgaccgcac ccacggcgat atcaggcggg ttaaccgggt
     8401 ggccggacca cggtgcgcga taggcccgat aggacatcac gcccccgggc agccgaccgt
     8461 tgaaccgcaa atcaccctcc ggcccaaact ccgagaaaac cggtgcgctt ccccatccga
     8521 cgaagaggtt gccgttcggc agcacctgca tatttccctg gcttacggat agaatcgccg
     8581 tcggatgaat gtactgatac gtcagggtcg cagtcatcgt ccccatatcg agatcgagca
     8641 ccatccctcg cgagttgccg ccaacacctg tacccgattc gtggttgtca aagagcgtca
     8701 gcgttccgtt cagttgcctt cgagcgtcat gctgccaggc gaaacgggcg ttcgggccta
     8761 tccggaagtc gctgcgcttg ccgtggagcc tccagataat ctcgcccgtc acccggtcaa
     8821 tcttgtagat ggcatgggta tgccggccgg agatgatcag gttgccgtcg gtatcgacct
     8881 caatcgaatt gatgtggaca tagtcgagcg gggtgctgag cctccgcggc gggccgatat
     8941 aggtttcctc gagatcgatg tgatccagtg aatgccactc gaagagcacc cgcccggtct
     9001 caatctcgat ctcctggatg attccgtcga gaaccgtgac attgtcaaga ccaccgattg
     9061 aggaacgatg ccacagcacg gggttataga tagccacgag cgccgttccc tgaggcgtga
     9121 gcaggaactc gtggacatct ccaccgggca ggccgttgcc gacctgtaac tcggcgattc
     9181 gctcgcagga gctgttgcag attacgaaat gaccgaagcc gtatccgaca ggcgcagcac
     9241 cctcccacca ggtcagaacc ggctggccac gatattcctg gacacggaag tcgttgcact
     9301 cgctgagatc aacggccggt ggcgcaaatc agacaagctc accgttctgg tccaggatca
     9361 tcgcgccgct ttggccgtca ggaatcctgg gcgcaacaaa gatatgaccc ggagcagtgt
     9421 ccacagcctc cgttgttacc tcgatcacag gcggtcgcaa atcaggccgc gatcgaaacg
     9481 tctgcgttgg gcccgccggt ttgttgacct cccggtacgc tgccggagca accttcgtcg
     9541 cgggtcgcgc gacggtgaac accaccgacc cctcggcact ctgccgcaac gcgacaccgg
     9601 ccgagacggt caccgattct cctggctcga aagccgcatc cgggaggaag ctgaccccac
     9661 ggccgtcggc atgctcgctc aggatgcccg agtgcccgcc gctctttgat cccgcggccc
     9721 gaacgggacc gagagatacg atcgataccc cccgaaacga gatttccgtt gcgggtgatg
     9781 cagtcatcgt gccatgcccg gggaagaccg atataccacc gggcgcgccc atgcgggaat
     9841 cagcatccgc cggatcggag agagcgtcct gcgccccggc gacgcggtcc cttgatggca
     9901 gaaccgcggc ggtcagcccc ccgatacctg ccgcccgcag gaatgaccga cgatcagtct
     9961 tcttcgcggc tccggcgaac ccctcacctg accttctcat cgtccatgac ctcctggaaa
    10021 acacgcccgc acagggacga agggcgctga attcggctca gaggagcagc gtgcctggtg
    10081 gatgatatgc ccgagcataa caaaagaggg cggatctggg agagcttcgt caggacggtc
    10141 cgccgcccgc gagccgcagc ccgaccacac cggcgatgat gagaactatc gaaaccagac
    10201 gtgctgcatc ccgcggctca ccgagaaggg cgatacccaa caccgcggtg cccgccgcac
    10261 cgatgccggt ccaaaccgcg taggcggttc ccacaggaag cgttttcagg gaatacgaga
    10321 gcatcgcgaa gctcagcact gcgaacacaa cgaacaggat gctcggtgtc caccggctga
    10381 aaccgtggct ctccttcagc gacagcgcca tgagcatctc aacaaaaccg gcaacgatca
    10441 agacgaccca gttcaccgcg gacctccata tgagaaaaag gggcacacgg agagcgatcc
    10501 cgatgccacg gcagtggccg gtgccaggat gcgatgcgtg gcgacccaac cgacttttgg
    10561 gactgcgaga ggtcagctgc tcacagggtc ctggcgcccc agaagcctgt cggcggccga
    10621 ctcaccagcg gcgatgatat cggggatacc aactccgcgc agcatgttac cggcaatctc
    10681 caggcccggc acccgttcta gtgctgcctc gagccgggcc acccgctcca gatgtcctac
    10741 cgtgtactgt ggcatcgcct cattcagacg atagacgcgc tgcacgaccg gatcgccgga
    10801 gatagcgctg acctcgcgaa gttcctggcg aaccagccca acgagctcat cgtcggagtg
    10861 ctggagcacc tcctgctggc ctacgcgtcc gatgaaggcc cgcaccagga aatgcccctc
    10921 cggtgcccgg tcctcccatt tcgaggaaag ccatgtgatg gccttgatgg gtcgctgttc
    10981 gacgcgcggc acgagatagc cgtagtcccg gggggcgatc acctcgccag tctcccggta
    11041 ccccagcagg accgccgcag aggaggcgac cggaatctcc gccatcgcgt gcgcagcctc
    11101 ggcatccagg tactgcagca atccggcctg cacacaaacc ggcgcggcga ggatcacctt
    11161 gtcgaagaga tcagaccagg cccggttctc cccgtcctgt gccacgacga accgaccgtc
    11221 gtccatctgg tcgatgcgtg tcacacggcg acgggtcttg atgacaccgc cgtgagtttc
    11281 gatagcggtc ttcagggcgt ccggcaattc atgcatgccg gtccggaagc tcacgaaccc
    11341 ctgcctcgtc tggtgaccga cagcacgcat ggtttgctgc ttgcgcccag ccagtacgcc
    11401 cttgatgagt cctccatgct ccttctcggc agcacgcagc tgggggaagg tcgctgccag
    11461 gctcagttgc gagccatccc ctgagtagat gccagccatc aggggttcga tcaggttgtc
    11521 gaagacttcg ggaccaagcc ggcggctgat gaacgcccga agggactcgt cgccatcccc
    11581 cttgcgagac gggagcacga aatccagcgc cattctcgcc ttgccgcgag gcgagacgag
    11641 cggcgtcttg accatcggcg ccagtttggt cgggatcagc ccggtcaggc cctcaggcag
    11701 cggatggagt gcgccctttc ggtagaggaa tgagccacgg tttgcctcat tggtccactg
    11761 caggcgatcc tccagcccga gctcccgggc cagtccaatc ccgcgtggtt tcatcaagag
    11821 gaaggaatcc ggtccgccat cgatggtgaa cccgtcgacg tgctctgtcc gcacaatgcc
    11881 gcccaggtgg tcgctggcct cgagcacgag gaccctaacg tcctgaccgg atgtcaccag
    11941 ccggtaggcg gccgccaagc cgctgatgcc gccgccgaca accgcaaccg tttgcattac
    12001 gccgttaccc agtccgcatt cctcgcgtga tcacggacga gctgggccag gccggccatc
    12061 atcttcggat ggtcgttgac catctggatg cgctcaagct gcatgcccaa ccccctcgcc
    12121 tgctgcgcga actcgacatc gatgtcgtag agaatctcga cgtgctcgca gacaaagcct
    12181 accggacaga tcagcacatg ctcgaccccg tcacgatgga gcctgtcgat cacctcgctt
    12241 gcgtccgggc caagccatgg gtccctggtc attgccgcgc tctggaaggc ccattcggcc
    12301 ggatgtcccg ggaatcggtc cttcattgcc acataggtcg cttccagatc gctctcgtag
    12361 ggatcgttcc attcgcgaat gcgttctggc aggctgtgcg cagtgaaaat caccggcaca
    12421 ttgtcccgca catcctcagg aaacttctgc aatgcgtcat tgatgcgctc ttccagtgcg
    12481 tcgagatagc ccggcaggag attccagcgg tcgattgcga ctgtctcgat accagaagcg
    12541 gcatcatcca cggccttgtt gtaggcgccg atcgacatcc tcgagtagtg cggggccatg
    12601 accagggtga cggcacgctc gaccccggcc tccttcattt tggagagcgt ctccgaaatg
    12661 taggggtacc agtggcgcat gccgtcccag actttccagg tctcgccgtc cggggcctgc
    12721 tcattcaatg cctgctcgat accgtcaccc tgggcacggg taagttccag gatgggtgat
    12781 cggccaccga tgagtttgta gcgctcctcg atctcgtgaa agacctcctc tgaggtgggg
    12841 cggtggtggc gtacatcgag gagatagggc cgcacatcct ctatcttgtc cggcccgcca
    12901 taagccataa tgaggaggtg gtgagtggct tgttcgcctg cggatgccgt tgattcgctc
    12961 gccatgtagt ggtccttatc agggtgtgga tgcaattcca tccacgacat aggtaccata
    13021 ccggagacaa acccggtgtc gggataagtt tgtgattcga gcggtgttcc ggtttgtcgg
    13081 acgttgccgg tcgatccgga gagaatcggc atggaaccag cagacagcaa acgggcacgc
    13141 atcctcaaca agcctgacct gatcacgctc atcgtcgtgc tcatgatcgt tcttgtcgtg
    13201 aacgcggtca cgctgatcgt tgtgttttag gcgacggtta gccgagacgg gaagaaagaa
    13261 ccccggacga cgttccgggg gcaggagctg ggcggcgtac cgcgactcag gcgtacacgc
    13321 ggacgatcaa ctcgccctcc ttgtcgacag cggtcttgcc ggctgtcacc ccggggcggc
    13381 gctcatggta ggaattcgag acgaggacca cggcatcggt cagcgcgcgc agcacgacgg
    13441 tatccccccg ctcggagaag gactccttca cctccagctc gccacgctgc ttgataatga
    13501 catgcttgaa ccagttgacc ggatcactga catccacgcc gttcagtcct gcctcattcg
    13561 cagcggcgat gagcgaatcg cgtgaggttg gctgtacggt cgttgccgtg gtggttgtct
    13621 caggcaccgg cagtgggccc gtgatcaggt catgccgccc cactgtgtcg tccataacct
    13681 cgaagagcgc ggttcgatcg gtaccgtaca gcttgtcgcc cttcttcaac acaatcgagg
    13741 cgttcgccgt catcgtcgtg gaggtggaga gtcgctcccg gcgatcctca ccgccgtacg
    13801 cgacaaaccc ggccacctgg tgaccggcaa gatcgatgat ctgtatcagc tgatttgccg
    13861 ccacctcgaa ggaaaccgcc gtcccgaccc cgacctgata tgtgcggacg acccgatgcc
    13921 cgacaagtac gtttgccatc gatgctttgc tcttcctgtc tggataccac gcgcctgcgc
    13981 cttgaatcgg ctcaccggcc cgaatactca tgcgccctat tgtcgcaggg cgaagccatc
    14041 atggtccaac cttgccccat tcctcagctc agcaccttgc caagggtgct gagcgcggga
    14101 ggataaaccg gtcggtaccg gcacaccggc aacggggttc gtccggcata ctgaccctgt
    14161 tgatcgtcac gagaccgata ccccggagcc agtacgttac atggccgaaa gcgcccttcg
    14221 ctcagttgtg cttgtcgcct tcaagcttgg ctgcatcgcc tttggtggac cggcagccca
    14281 tattgcgatg ctgaggcaag aggtcgtcga gcgacgcaga tgggttgagg agcagcgctt
    14341 cctcgatctg atgggcatta ccaacctgat cccggggccg aactcaactg agctcgtgat
    14401 ccacattggc cgggaacggg ccggcatcaa gggactgatt ggcgccggag ccgcgtttat
    14461 cctcccggcg gcgtcaatca cgctcttctt cgcctggctc tatatgtcct acggaacgac
    14521 gacgcagggg gagtggctgc tgcgcggcat caagccagtc gtcctcgcgg tcgtcatgca
    14581 agcgatctgg aacctcggca agacggcctt caagacactg ccgctcacga ttgtcggcgc
    14641 gttggtattt gtgctctatc tgcttggtgg taatgagatc ctgctgctct tcggcggggc
    14701 gctggtcgcg ttcctcatgc agcgcatcct ggcgtccggg caccggtcgt cggcatcatc
    14761 attcgtcgcc ccatttctcg tgccgggcgc gttctcgctc gtcctcgcgg atgtcgcgac
    14821 cgttccctat tctgccctcc gcctcttcct gaccttcctc aagatcggca gcgtgctcta
    14881 tggctcgggc tacgtgctcg ttgccttcct gcagaacgat ttcgtcgagc gtcttggatg
    14941 gctgtcggag aagcaactcc tggacgcggt tgctgtgggt cagttcaccc ccggaccggt
    15001 cttcacgacg gcaacctttg tcggttatgt cgtcggcggg ttccccggcg ccgtcctcgc
    15061 tacgctcggc atctttctgc cagcgtttat cttcgtcggg atcgcccatg cactgatcga
    15121 ccggctgatg ggactcacgt gggtacgccc acttctggat ggcatcaacg tcgccgcggt
    15181 gggtctgatg gcggcggtcg ccgtcatcct cgcgcaagat gcggtcgtcg atatcatgac
    15241 cgccgtcctc ttccttgcag ctctcgcatt gctgatccgc tacaaggtca actcggcggt
    15301 cctcgttgcg gcgggagcgc tcatcagcct ggtccgggag ttcttgctct gatgcgtggt
    15361 gcatacaggc tatggcgata accccgtcac ctggagcggg caaaccatcc ctacctccgc
    15421 agggtcgaca atcgggcagg gagcatgcga ttgtagcggg cagagagcac agcctgtttc
    15481 gcgatcggcg ttccaccagg agggttcacc atggagcgat gttccaactg cggcgcggcc
    15541 accagacctg gcgcgaagtt ctgcacgtct tgcggcaccc gtctcaacct tgatcaggag
    15601 tctaccgggc cgacggattc ctcgtggaac agtgcgtcct cgacccaaga gacgtcagtt
    15661 gcgacgccgg caatcgagga tggagacatc accacggaga gggatgccgt tacccctccg
    15721 ccacattggg aggagacggt ccaggacccg cgaccagaaa atggagacga agcgcctgca
    15781 gcggcatggt cgtcgggatc accatggaca gcgggagagg cggagccgag tgccgaagca
    15841 gaggcgcaga acgtgagggg cagcgaggac tccgactggt cgagcaagtg gcactcggaa
    15901 gagagagtcg atgaggcggt gggcactggc agtagcaccg actccacgtc ccccgaagac
    15961 aatcgctggg ca
//
LOCUS       DASYWM010000120         6810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_360874, whole genome shotgun sequence.
ACCESSION   DASYWM010000120 DASYWM010000000
VERSION     DASYWM010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360874"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..2520)
                     /locus_tag="VGR29_05015"
     CDS             complement(43..2520)
                     /locus_tag="VGR29_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2072985.1"
                     /translation="MILSLPADGQTTLPVPPNRLVGREQDCTALAKLLEDQHTRLVTL
                     TGPGGVGKTHLSLELGATVASRFPDGVVFVSLSSIQDPELVLPAIAQALGLRETAGQP
                     LVHLIRDAITGQDMLIVIDNFEQVMQAGPHIGVVLATTRTPKFLVTSRSPLRLRMERE
                     YPVRPLPLPPPLLASTTGSLEDNAAVALFVERAQAARPSFALTDTNAAAIVEICRRLD
                     GLPLAIELAAAMTRVLSPHALLARMEHRLSLLIAGATDLPDRQRTLRDTIAWSHDLLT
                     TEEQMVFRRLAVFVGDASLDAIEAVAGDPGAASGSTTAIQKIDVLSVVSSLVNKSLIV
                     QMEDGDSTESSMDAPRFRMLATIQEFATEQLEASGEVEAVRQLHLDWLIRFAEKAEPQ
                     LTGPDQAQWLVWLDAEHDNIRAALTWAQAHCHQEGMRLASMLWRYWATHGLLTEGHTW
                     LCRFLDSPAHAEESVRAKAFSSLGNLSLDLGDYHAASEAYQEALDAWERLGHTRGIAD
                     ALNGQGLVDWYRGDYDSARRRHERSLELRRSIGDRFGQGNSLTNLGNAVKDSGDPVAA
                     RELHQQALAIRQSLGHRVGVGYSYLNLGHVARRLGDVAEARAMFSKCLDTFQDIGDTL
                     GVGYALHGLGLADMLSGDARQAGVNFLGALEIRLGLGDRRGVVESIEGIASVAASLGQ
                     PPVAATLFGATSALREQIGAPLPAPDRPRHEKLVESVRRSLPLAAFSSSWKTGTGLSL
                     PEAADLALSAAREVTSAPEQPASMLSGREVEVLQLVAAGMTNAQVAEQLFLSRRTVDA
                     HLRRIYDKLDLSSRAEAIRFAMDGGFV"
     gene            complement(2663..3607)
                     /locus_tag="VGR29_05020"
     CDS             complement(2663..3607)
                     /locus_tag="VGR29_05020"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV2072986.1"
                     /translation="MTILLTALGLAFIVLTLRDIFHTLFAPSGTGAMSSFAARHVWRA
                     FRRFARHRSGMLALAGPLGLLCIIALWALALILGWALIIWPHLPEGFLLAPGMEPDQN
                     GGFLTAFYLSLVTLATLGYGDITPKSSWLRILVSAEAVVGFALMTAAISWILSVYPVL
                     ARRNHLAREVLLLRRSERHGSITLTGTANQGLDAVLLNLTDQVIAFQNDLAQSPITYY
                     FYNANPHSAIEVALPELASIARDARDDHSPSVRFQAALLLEALTELASYLGETFLEGE
                     AETLEEMLTAYAADHGRELSDAAGEDDVAVDARTERLR"
     gene            3963..4361
                     /locus_tag="VGR29_05025"
     CDS             3963..4361
                     /locus_tag="VGR29_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2072987.1"
                     /translation="MRADGTQPHVLAVNDSPELLDLFCEIFDEECFRVTTRAEGDTKF
                     DDIVQLAPDLIILDYRVGVEGVLLLQLTESPVTNHMPIILCTGVVRNEADAVWKLLNS
                     MGVTIVFKPFDIEHFLRVVREVLGLDSDLD"
     gene            4453..4884
                     /locus_tag="VGR29_05030"
     CDS             4453..4884
                     /locus_tag="VGR29_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2072988.1"
                     /translation="MDADTDQHARGRATPEQMARQHIYVMNGSPEFLDIIRALLQEER
                     YNVTTTNFIPNSFRTIETAQPSLLIIDLIVGEQAGWDLLGRLRVSVSTREIPVILVST
                     TPRLLDEAREQHEIFGGDHYLVKPFDLEDLLRLIGELIGTA"
     gene            5585..5890
                     /locus_tag="VGR29_05035"
     CDS             5585..5890
                     /locus_tag="VGR29_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072989.1"
                     /translation="MSTSAPSIHIPTRPSDTLVAPSGQSFPVTTGSPHAAQLEQVHRE
                     LERMTAYARALETRCNVLRVYVPGNQGSQDPIVPFLADDLGITATTVTHVEILASHD"
     gene            6088..>6810
                     /locus_tag="VGR29_05040"
     CDS             6088..>6810
                     /locus_tag="VGR29_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2072990.1"
                     /translation="MTLHGDRVNIFGRSGSRISRRRAIGYGSLALAGALIGPSVVQPV
                     AAQASAATPISSAILAWEEIDQQLAEVAPTTALLAAELIGDRIESIHETNADQALPIG
                     STFKLWILGALSLQVEAGTLDWEQMVEIQDAHRSVPGGDLRYVRAGSSFTLRYLAERM
                     IQKSDNTATDHLLFLAGRENVDQAMATMGHSDPAHNIPLLSTRELEMLKFAYPQEQLD
                     AYYAASVDERRRILAEEIAAIPY"
BASE COUNT         1372 a         2070 c         2074 g         1294 t
ORIGIN      
        1 ggggtggacg tgtgttcctg attgggatcg gagaggacgg gcttagacga aaccgccgtc
       61 catcgcaaag cgaatggctt cagcccgcga ggagaggtcg agcttgtcat agatgcggcg
      121 gaggtgggcg tcgacggtgc gacgactcag aaagagctgt tccgcgacct gggcattcgt
      181 catacccgca gcgaccagct gcagcacttc aacttcacgc ccgctgagca tgctcgcggg
      241 ttgttccgga gccgaggtga cctcccgggc agcagacagg gccaggtccg ccgcttccgg
      301 cagcgacagc ccggttcctg tcttccagct ggaggagaac gccgccagag gcaacgaccg
      361 gcgaaccgat tcgaccagct tttcatggcg cggccgatca ggcgcaggca agggcgcgcc
      421 gatctgctcc cgaagtgcgc tcgttgcgcc gaacagcgtt gcagcgacag gtggctggcc
      481 cagtgaagcg gcgacagatg caatgccttc aatgctctcc acgacaccac gtcgatcccc
      541 gagtcccaac cggatctcga gggctccgag gaaattcact ccagcctgcc gcgcatcacc
      601 ggaaagcata tccgccagcc cgagcccgtg aagcgcatag ccgacaccga gcgtgtcccc
      661 aatatcctga aacgtatcga gacacttgct gaacatggca cgagcttcgg caacgtcccc
      721 gaggcgacgc gcgacgtgtc cgaggttaag gtaggaatac cctacaccaa cgcgatggcc
      781 gaggctttgc cggatcgcca gcgcctgctg atgcagttcc cgggcggcga ccggatcgcc
      841 agaatccttg accgcgttgc ccagattggt cagcgagttc ccctggccga accggtcacc
      901 tatgctccgg cgcagctcca ggcttcgctc gtgacgacgc cttgcggagt catagtcacc
      961 ccggtaccag tccaccagcc cctgtccgtt gagcgcgtcg gcgatcccgc gcgtatgtcc
     1021 gagccgttcc catgcgtcca gggcttcctg ataggcttcc gatgcagcgt gataatcacc
     1081 aagatcgagt gagaggttcc ccaggctgct gaacgccttt gcccggacgc tttcctcggc
     1141 atgggcgggt gaatcgagga acctgcacag ccatgtgtgg ccctcggtga gcaggccatg
     1201 tgtggcccag taacgccaaa gcatgctggc gaggcgcata ccctcctggt ggcagtgagc
     1261 ctgggcccag gtcagcgcgg cgcggatgtt gtcatgttcc gcgtcaagcc acaccagcca
     1321 ttgcgcctgg tcagggccgg tgagttgcgg ctccgctttt tcggcgaacc tgataagcca
     1381 gtccaggtgc agctgacgaa cagcctcgac ctcaccactt gcctcgagtt gctccgtcgc
     1441 gaactcctgg atcgtcgcca gcatccggaa ccgtggagca tccatggagg attctgttga
     1501 atcgccatcc tccatctgta cgattagact cttgttcaca agggaggaga ccaccgagag
     1561 tacatcaatc ttttggatgg cagtggtcga gccgctggca gcaccagggt caccggcgac
     1621 ggcttcaatt gcgtcaagtg atgcatcgcc cacgaagacc gcaagtcgcc ggaagaccat
     1681 ctgctcttca gtggtgagca gatcgtggct ccaggcaatc gtgtcccgaa gtgtgcgttg
     1741 tcgatccggc aggtctgtgg cacccgctat cagcagtgac agccggtgct ccatccgggc
     1801 gagtagtgca tggggcgata gcacgcgcgt catcgccgca gcgagctcga tcgcgagagg
     1861 tagaccgtca agccgcctgc atatctccac gatcgccgct gcattcgtgt cagtcaacgc
     1921 gaacgaagga cgtgccgcct gcgcacgctc cacgaacagc gcgacggcag cattgtcctc
     1981 cagcgatccg gtggttgagg caagaagggg cggaggcaga gggagcggac ggaccgggta
     2041 ttcacgctcc atgcggagac gcaacgggga acggctggtc accagaaatt tgggcgtcct
     2101 ggtggttgcc aacacaacgc ctatatgcgg tcccgcctgc atgacctgct cgaagttgtc
     2161 gatcacgatc agcatatctt gacccgttat ggcatcgcgg atcaggtgaa ccagcggttg
     2221 accggcggtt tcgcgcagcc caagggcttg ggcaatggcc ggaaggacga gttccggatc
     2281 ctggatcgaa gacagggaca caaacaccac tccgtcgggg aagcgtgatg caaccgtagc
     2341 tcccagctcc agtgacaggt gtgttttccc gacaccgccc gggccggtaa gtgtgaccag
     2401 acgggtatgc tggtcttcca gcagtttagc gagcgctgtg caatcctgct cccggccgac
     2461 aagccggttg ggaggaacgg ggagcgttgt ctgaccatca gcagggagcg aaaggatcat
     2521 ggccggtcca tctatttcat ggtgttctca ctcaaaagtc ccatcaacaa tatacggcat
     2581 aatgcctggt ctttgatagc caggggcgag aaagaggggc aggaagagag tgagtgggcc
     2641 ttggttcagt ggcgtgatcc gcttacctca gacgttccgt ccgcgcgtct accgcgacgt
     2701 cgtcttcacc ggcggcatcc gagagctcac gcccgtggtc ggcggcgtat gcggtcagca
     2761 tctcttccag cgtctcggcc tcgccctcaa ggaacgtctc tcccaggtaa ctggcgagct
     2821 ccgtgagcgc ctcgagcagc agggccgcct gaaatctgac agaaggcgaa tgatcgtcgc
     2881 gcgcatcacg ggcgatcgac gccagttctg gcagtgccac ttcgatcgct gaatgaggat
     2941 tcgcattgta gaagtaatac gtgatgggac tctgggcgag atcgttctga aatgcgatca
     3001 cctgatccgt gaggttgagc aggacggcgt caagaccttg gttggcggtg cccgtgagcg
     3061 tgatactgcc atgtcgctcg ctccgcctga ggagcaggac ctcccgggca aggtgattcc
     3121 gacgggcaag tactgggtag accgagagaa tccaggagat cgcggcggtc atcaatgcaa
     3181 agccgaccac cgcttccgcg gacacaagaa tcctgagcca ggagcttttc ggagtgatgt
     3241 ctccatatcc gagcgtggcc agcgtgacca gcgaaaggta gaacgcggtc aggaacccgc
     3301 cattttggtc aggctccatc cccggggcga gcaggaaacc ttcaggcagg tgtggccaga
     3361 tgatcagggc ccagcctagg atcagcgcga gcgcccagag cgcgataatg cacagcaggc
     3421 caaggggtcc ggccagtgcc agcattcccg atcgatgacg tgcgaaccgc cggaaggcac
     3481 gccatacatg ccgagcggca aagctactca ttgcgccagt cccggatggt gcaaagagcg
     3541 tatggaagat gtcgcgtaaa gtgaggacga tgaacgcgag gccaagggcg gtgagcagga
     3601 tcgtcaatgg ttgttccccc ggcgagttct ggaacgatgg tcaactggtc gtgtatccgg
     3661 cacctcgttc cgctaatatc cggtcctgag cgtcaagcag cgacttccac gtttttcgtc
     3721 acgcaaatgg cccctacggc agtctcaccg gtggccaacg ccgcattgtc gggatgcaac
     3781 agatatgcga gtctcttcct gatggtcaga cgtatgccgt cgactgccaa gtcatacaat
     3841 actggcttca actattcatt tctctccacg gccatctgga ctctgtacca cgccactgtg
     3901 ggagcatctg tgccgcaccg gacatctgca gtcggatgcc acagtgccgg gaagggaacc
     3961 gcatgcgcgc tgacggtacc caaccacatg ttctcgcggt caacgactcc cctgagctgc
     4021 ttgacctgtt ttgcgagatc ttcgatgagg aatgctttcg cgtcacgaca cgggcggaag
     4081 gtgacacgaa gttcgacgac atcgtgcagc ttgcgccgga cctgatcatc ctcgactacc
     4141 gcgttggtgt tgagggggtc ctgttgctgc agcttacaga gtcgcccgtc acgaatcaca
     4201 tgccgatcat cctgtgtacc ggagtggtgc gaaatgaggc cgacgcggtg tggaagctcc
     4261 tgaactccat gggcgttacg attgtgttca agccgtttga catcgaacac ttcctgcgcg
     4321 ttgttcggga ggtgctcgga ttggattctg atttagatta gccactgaag cacggacgtt
     4381 tttgtttcgg ttggttccgg cgttcgaggt cggacgccct cacactcaac cgagatcagg
     4441 ggagtagccg gcatggatgc ggatacagac cagcacgctc gcggacgggc cacgcccgag
     4501 cagatggcgc gccagcacat ctatgtgatg aatgggtcgc cggagttcct cgatatcatt
     4561 cgcgcattgc tccaggagga gcggtacaac gtcaccacca ccaactttat ccccaactcc
     4621 tttcggacga tcgagaccgc gcagccgtcg ttgctcatca tcgacctgat cgttggcgag
     4681 caggcggggt gggacctgtt agggaggctg cgagtgtcag tatcgacccg agagatcccg
     4741 gtgatcctgg tttcgaccac gccgcggtta ctggacgagg caagagaaca gcacgaaatc
     4801 tttggcgggg atcactatct ggtcaagccg ttcgacctgg aagatcttct ccggctcatt
     4861 ggagaactga tcggcacggc ctaggccgcc agaaacgctt tcgtattgtg acgtggcggg
     4921 tctccggcat cgtcggagtt ggggcggaac cctctttctc aggcactcct gcagcgctgt
     4981 ccggtcgaaa ctacgtaggt tctacgtaat caacagcgac aaaaatacgg acttttaccg
     5041 atgcccctca ttccgggaat agggatactg taggggaccc ttccggagac ggggaggact
     5101 cttggcaccg ggcacggacc cagcctggta ccaagggagt gccgaccagt gctccaggcg
     5161 tgacatcaat cgatcccgtg tggtgctgga tcctgatgcg cgcggttggt cgcctgtcga
     5221 ggagaggctc cgttgccgga gcacgacagg gacgccaatc tattccccct tcctccaccc
     5281 acaggaagac gccggatgcc cccttcgtcc ggcgccttcg tgcttcctgg cggtgcgtcg
     5341 cccgaccgga caacgaccag caacatcgct cctgcttcgc gcggagcatt atcgtttcag
     5401 acccgcccgt actacggctt cccctgatta cgtactcact ccacccacaa taccgtattt
     5461 ctacggtatg tggcccggca ggttatccgc catgattgac acagatttca ggatctgctc
     5521 tggaaaacac ggatcccatc tcccgcacgt tttatcgctg ctgcggcagc atgcgaggct
     5581 ccccatgtcc acaagtgctc cttccatcca catcccgacc cggccctctg acaccctggt
     5641 cgcgccatcg gggcagtcct tccccgtgac aaccgggagc ccccatgccg cgcaactgga
     5701 gcaggtgcac cgggaattgg agcgaatgac ggcgtacgcc cgggcgctgg agacccgatg
     5761 caacgtgctg agggtctatg tgccagggaa ccagggcagc caggatccga tagtcccctt
     5821 ccttgccgac gacctcggga tcacagccac cacggtgacg catgtggaga tcctggccag
     5881 ccacgactga tcggggagct gtccgggaac tcggcccggg agttgaggac aacaaggtcg
     5941 tcttccaagc atcggccatt cacatcgata gttccagaca cggccctcga tacatccggg
     6001 gacatggtct ttcgcgccag ctgcttcgtc gagtctgacg accggcatga cgggtggccg
     6061 cacgcatata cggtaagcac ggaacgcatg acactccacg gtgatcgcgt taacatcttc
     6121 ggccgctccg gtagccgcat ttcacgccgc agggcgatcg gatacggcag cctggcactt
     6181 gccggggcac tcatcgggcc cagcgtcgtg cagccagtgg cggcacaggc aagtgcggcg
     6241 accccgataa gctctgcaat cctggcctgg gaagaaatcg accagcagct ggcagaggtc
     6301 gctccaacaa ccgcattact ggcagctgaa ctgattggcg acaggatcga gtcgatccat
     6361 gaaaccaatg ccgaccaggc tctcccgatc gggtcaactt tcaaactctg gattctcggc
     6421 gccctgtcgt tgcaggtcga ggcgggcacg ctcgattggg agcagatggt tgaaatccag
     6481 gacgcgcacc ggagtgtgcc cggcggtgac ctccggtatg tcagggccgg tagcagcttc
     6541 accctgcggt atctcgccga gcgaatgatc cagaagagcg acaacaccgc gaccgaccac
     6601 ctgcttttcc tggctggtcg cgagaacgtc gatcaggcga tggcgaccat gggtcatagc
     6661 gatccggccc acaatattcc cctgctgtct acgcgagagc tcgagatgct caagttcgcg
     6721 tatccccagg aacaactcga tgcctattac gctgccagtg tggatgagcg tcgccggatc
     6781 cttgccgagg agattgcggc gatcccatac
//
LOCUS       DASYWM010000121         5442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_23036, whole genome shotgun sequence.
ACCESSION   DASYWM010000121 DASYWM010000000
VERSION     DASYWM010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23036"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VGR29_05045"
     CDS             complement(<1..988)
                     /locus_tag="VGR29_05045"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072991.1"
                     /translation="MRVADLRFTASEATEFLNPVMGLDLSAADIAALEGRTEGWIAGL
                     QLAALSMQGHQDATGFIQSFTGSHHFVMDYLVEEVLRQQPEYVQSFLLSTSILDQLCG
                     PLCDAVVLDPSASSQQTLEYLEWANLFLIPLDDERRWYRYHHLFAELLRQRLHQGSAS
                     KSGDEVADVVGLHHRASVWYEEHGLEVDAFQHAAAAHDVARAERLIEGDGVPLYFRGT
                     VAPVLKWLESLPRPVLDARPSLWVMYASGVLFVDHTAVEQKLQSAEAALHGVELDDST
                     RDLIGRIASLRATLAVIQNDEEAIIAQARRALRYLHPGNSIVRTGTAWSLGYA"
     gene            complement(975..1523)
                     /locus_tag="VGR29_05050"
     CDS             complement(975..1523)
                     /locus_tag="VGR29_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2072992.1"
                     /translation="MSTQVLVTKLYVPPPRTRAILRRRLVERLNEGMDRKLTLISAPA
                     GFGKTTLVSEWIASGRPAAWLSLDEGDNDLSRFLAYLVAALQTIGPTIGERVARALQL
                     PQLPPTESILTTLLNDISALESQAVLVLDDYHRIESIAIDAAVTFLLEHLPPQMHLVI
                     TTREDPQIPLARPGPTDRVARR"
     gene            1993..2838
                     /locus_tag="VGR29_05055"
     CDS             1993..2838
                     /locus_tag="VGR29_05055"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEV2072993.1"
                     /translation="MSFPMADARSEVFHRFTTACQDDARIQAAFLGGSHGAGTADAYS
                     DLDLYLFIADESYEDFFADRANFLRRLGEPVFQEDFNGFGFDLMAFILANGVDGELVL
                     ERSSRADNIQNGPFVPVVDRGGLLQERDFPIRLPSAAERADVLRMALLWFWRDIIHIT
                     RAIARDRPWTAYGYLEEMRHKCVDLLTLAQGRDEESPEPFVPVNGFAVLERVTYEAEL
                     ALLRSSLGPLDRSALLASTRALIQIYQRLGPPLAKHHGLPYPNQLAALTLKQFDGMDA
                     AMRGE"
     gene            3119..3496
                     /locus_tag="VGR29_05060"
     CDS             3119..3496
                     /locus_tag="VGR29_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2072994.1"
                     /translation="MSVTFDVIGIAVRDMRASLQFYRRLGLPVPDGMEGEMHVEMSLG
                     GIRLAWDTVEMLQDVYNGWDEPSGHCVELAFRCDTREEVDEVYDRLMQDGYRGHKEPW
                     DALWRQRYAIVKDPDGNLMSLYA"
     gene            complement(4153..4231)
                     /locus_tag="VGR29_05065"
     tRNA            complement(4153..4231)
                     /locus_tag="VGR29_05065"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            4912..>5442
                     /locus_tag="VGR29_05070"
     CDS             4912..>5442
                     /locus_tag="VGR29_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2072995.1"
                     /translation="MTDTSKRVLLTGATGYIAGQLLPALRERYDLRLIDVRKENRDGE
                     PVPGVETVDLMHADRSTLEELFSGVDTVIHTGYIRPRPEAVYEDERKNVDMMQRVYDV
                     ALGTGVKRVVAASTNQAAKWYEQPYYAGRRDRVTPEDYPRPDSFYGWAKAAYESLGFL
                     YASGSLGRKLEVIQIRI"
BASE COUNT         1074 a         1597 c         1703 g         1068 t
ORIGIN      
        1 gagcataccc gagtgaccag gcagtaccag tccggacaat cgagttgccg ggatgcagat
       61 agcgcagggc gcgacgggcc tgggcaataa tggcttcttc atcgttttgg atgacagcca
      121 gcgtggctcg cagggatgcg atgcgcccga tcagatcgcg ggtgctgtcg tccagctcga
      181 cgccgtgcag ggccgcttcg gcagactgaa gtttctgttc gacggcggta tggtcgacaa
      241 acaatacccc tgaggcatac atcacccaca atgagggtct ggcatccagc actggtcggg
      301 gtagcgactc gagccacttc aggactggtg ccactgtgcc ccggaagtac agtggcactc
      361 cgtcgccctc aatcaggcgc tcggcgcgcg cgacatcatg ggccgcagcg gcgtgctgaa
      421 acgcatcgac ctccaggcca tgctcctcat accagacgct ggctcgatgg tgtaacccga
      481 cgacatccgc cacctcatct cctgatttcg aggcactccc ctgatgcagt cgctgccgta
      541 gcagctcagc gaagagatgg tgatagcggt accagcgtcg ctcgtcgtcc aaagggatga
      601 ggaagagatt ggcccactcg agatattcca gcgtttgctg gctggaagca gaggggtcga
      661 gcacaacggc atcgcataga gggccacaca gctggtcgag gatagaggta ctcagcagga
      721 atgactggac atattcgggc tgctggcgca ggacttcctc caccagatag tccatcacga
      781 aatggtggct gccagtgaac gactggatga agccggtggc gtcctgatgg ccctgcattg
      841 aaagcgcggc caactgcaga ccagcaatcc agccttcggt gcggccttcc agggcggcga
      901 tgtcggccgc agaaagatcc agtcccatca ccgggttgag aaactcggtt gcttccgatg
      961 cggtaaaacg caagtcagcg acgcgcaact cggtcagttg gccccgggcg ggcgaggggg
     1021 atctgtggat cctcgcgggt ggtaatcacc agatgcatct gcggaggcag gtgctcgagc
     1081 aggaacgtga cggcggcgtc gatcgctatc gactcgatgc ggtgatagtc gtcgaggacg
     1141 agcaccgcct gggactccag cgcgctgatg tcattgagca gggtcgtgag gatcgactca
     1201 gtcggaggca gctgcgggag ctgcaaggcc ctcgcgaccc tttcaccgat cgtcggcccg
     1261 atcgtctgca gggctgcgac aaggtacgca aggaatcgcg agaggtcgtt gtcaccctcg
     1321 tccagcgaga gccacgcggc cggtcgaccg gaagcaatcc attcgctgac caacgtggtc
     1381 ttgccgaaac cggcgggcgc cgagatcagg gtcagcttgc gatccatgcc ctcgttcagg
     1441 cgctcgacca gacggcggcg aaggatagcc ctcgtccggg gcggcgggac atacagcttg
     1501 gtgaccagca cttgagttga cattgtctgt atttggcccc tgggatcgtg atcaagggga
     1561 tctggaccat ggagcatttg cccggacgtg ctgttttgca gtcggcgtga ccaagtcaca
     1621 agagatttgc gaagctaccc atcagtgggc ccatacctgc catgcttacc aggacgcttg
     1681 agtacgaaga cagatgaaga tcgcgaaggg gagaatgccc tattcgtgga gcatggcttg
     1741 gaggtcctga ttcattatcg tggattggac gcggttatgc atcggccaaa gggtggagag
     1801 atgcctccct cgagtggcaa ggcagagacc ttcatccctc gtcgcaccgt cccgagtaca
     1861 cacgtgacgg ccgggtgcct ctctatcacc acgaaattgt tgccctagga tgcaggtgtc
     1921 cagggtcttc cattggacag cccaagtggt aatgtcctca tcattgacgc ctcttcacac
     1981 aggatgcctt ccatgtcctt cccaatggcc gatgcccgca gtgaggtgtt tcaccggttt
     2041 acgaccgcct gccaggacga tgctcgcatc caggccgcct ttctcggtgg gtcccatggt
     2101 gcaggcacgg ccgacgcgta ttccgatctc gatctctacc tgttcattgc cgacgagagc
     2161 tacgaggatt tcttcgccga ccgggccaat ttcctgcgcc gcctcggcga acccgtcttc
     2221 caggaagatt ttaacgggtt cggcttcgac ttgatggcct tcatcctggc taacggcgtg
     2281 gacggcgagt tggtgctgga gcgatcgtcc cgtgccgaca acatccaaaa tggtcccttc
     2341 gtgccggtcg tcgaccgtgg tgggcttttg caggagaggg atttcccgat ccgcctgcca
     2401 tcggccgccg aacgtgcgga tgtccttcgc atggccctcc tctggttctg gcgcgacatt
     2461 atccacatca ccagggccat tgcccgtgac cggccctgga ccgcctatgg gtatctcgaa
     2521 gagatgcggc acaagtgtgt cgatctgctc accctggcgc agggacggga tgaggaatca
     2581 ccagagccgt tcgtgccggt gaatggcttt gcggtgctgg aacgggtgac atacgaggcg
     2641 gaactggcgc tcctgcggtc gagtcttggt ccgctcgacc gatccgcact gcttgcctcc
     2701 acacgcgcgt tgattcagat ttaccagcgg ctggggccgc ctttagcgaa gcaccacggg
     2761 ctcccctacc ccaatcaact tgccgcgctg acgctgaagc agttcgatgg gatggatgca
     2821 gccatgagag gcgaataagg ggaccaacgt tctggaagga caccggtttc tgcggtccgg
     2881 cagttccttg cacgtgtgag aaggatctcc agacacgtta tgcggagcat ttcccatctt
     2941 atatctcccg gcgcatcata gccctcgatg tgtcgcaccc ggataaccac gtttcaggcg
     3001 atgcgggtgc cagcggccag ggaggacgag gtgcccggta gttgcgctgg tgggcgcgtt
     3061 ctgcatgaac cggcccccat ccgggacatg atttgccctt catacaaagg agaggtcgat
     3121 gtcggtgacg ttcgatgtga tcgggatcgc cgtaagggat atgagggcgt cgcttcaatt
     3181 ctaccggcgg ctcgggctcc cagtccccga cgggatggag ggggagatgc atgtggagat
     3241 gagcctcggt ggtatccgcc tggcgtggga tacggtggag atgctccagg acgtttacaa
     3301 cgggtgggac gaaccatctg ggcactgcgt cgaactcgcc tttcgctgcg acacccgaga
     3361 ggaggtcgac gaggtgtatg atcggctcat gcaggacggc tatcgcgggc acaaggagcc
     3421 atgggatgct ttgtggaggc agcgctatgc cattgtgaaa gatccggacg gcaacctcat
     3481 gagcctgtac gcgtaacccg acatcgtgcg aggtgatgtc tctgagcatg atgggacctc
     3541 aacggtccgt acctgcatat ggaagggctc accgacagac gttacccccc gcggagcagg
     3601 atcgaccagc gacagggtgt cccctctcac gggtgccacg cctgagtggt gcggaaagcg
     3661 cgaaaacgcc ggacgaaggg ggcgtccggc gtcttcctgt gggtggagga agggggaata
     3721 gattgtcatc cctgtcggac tccggcaacg gagcctctcc tcgacaggac accgaccacg
     3781 cgcatcagga tccagcacca cacgagatca tgtgatgtcg cgtccggcga ctcgggagag
     3841 gatccgctgg caccacggtg gctgggtccg tgttcggtgc cgggtcgcct ccccgtctcc
     3901 ggaagggtca cccctagtgt gcctggttcc ccgacatggg gcatccgtaa aaatacgtat
     3961 ttccccgccg accaattacg tagaccccac gcaaaggaag gaccgacaac tctcgcagac
     4021 gcagaaaaga ccggatgagg ggtcatcgtg ccagccgcac tcgccctcat ccggtcttct
     4081 ccgggagaga atggtatgcc ctttcgctcg gtcccgtggt atgggtgtgg tgctcacggt
     4141 acccgcgact agtagaggtg gctggactcg aaccaacatc cgtggccatg cggttgccgc
     4201 ccgccacact cgtccattga gctacacctc ttgcaagttc agtgacgcgg atgcatccgc
     4261 gtctccaagt ttcctcacga tctcctgaac gtccgggacc cttcggaagt tcccatgtgg
     4321 tccctcatgg tgcgcaatcc gatcatcacc gtgatgtgac tgcgtctcct cccttgcgga
     4381 caatagagat tgccggtgtc ccttttcgca tcatagcaga cgagcaggcc aaatagatag
     4441 aggtgttccc catcaattgt catgacagtg gccctcgcgg aactcccaga cgctatgtac
     4501 aacagctcag cagcataggt cagccaggaa acaacttctt ctcgccggca taccagcccc
     4561 tctgactgcg tcgcggagat ggagcagccg gggacgcgga tggcggggcc gacttcacca
     4621 ggcgggacac cggctggtgt atctctcctg cggaggcgaa ctggcaccag cgatcgtagc
     4681 ggtctgcttt cagattcccg tgttgaggga ccgtttccag ccgaatgggg ctaagatgcc
     4741 atctgactga gccggtggtg gatctcccca gaggtggatg gttgatacca gcgggccgca
     4801 ggacaggtca taccccctac cctgttgccg ttccacatgg gaatcgtgag cttcgtgttg
     4861 gttcggatga tggccggaaa gtgacagatc aagcggaagg atatttcccc gatgaccgat
     4921 acctccaaga gggtcctgct gactggagcg accggctaca ttgccgggca gctactcccc
     4981 gcacttcgag agcgctatga ccttcgcctc atcgatgtca ggaaggaaaa ccgggatggc
     5041 gaaccggttc ccggtgtgga gaccgttgat ctgatgcacg ccgaccgatc aacgctggag
     5101 gagctattca gcggtgtcga cacggtcatt cacaccggct acatccgtcc ccggcccgag
     5161 gcggtctatg aggacgaacg caagaacgtg gacatgatgc agcgggtgta cgatgtcgcc
     5221 ctcggtaccg gggtcaagcg cgtcgtcgcc gcgagcacca accaggcggc gaagtggtac
     5281 gagcagccct attacgcggg acggcgtgac cgcgtcacgc cagaggatta ccctcggccg
     5341 gactccttct acggatgggc gaaggctgcc tatgaatcgc tcggctttct ctatgcatct
     5401 ggatcgctgg gccgcaaact ggaggtcatc cagattcgga tt
//
LOCUS       DASYWM010000122        10325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_211215, whole genome shotgun sequence.
ACCESSION   DASYWM010000122 DASYWM010000000
VERSION     DASYWM010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211215"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1175
                     /locus_tag="VGR29_05075"
     CDS             246..1175
                     /locus_tag="VGR29_05075"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEV2072996.1"
                     /translation="MTLQHQGTQWPTLPYDEWQATQATLHRWMQIVGKVTLEGTPFLN
                     EWWNVGLTVTSRGMTTPLIPYGQEAFTIAFDFIDHTLSIDVTDGRTGRLPLRPQSVAS
                     FYLEFFNTLEALGIDMPINPMPVEIVDPVSLDFDEGNDAYDADAVYRWWRILFQTEKV
                     LQRYRSSLAGKSSPVLFFWGSFDLAATRFSGHPASLPPGAPRFMQLAEDQENIAVGFW
                     PGNDNYAGVPLGEPAFYAYIFPEPPGFKEATIRPAEAAYRPELGQFLLPYEAVRQAPA
                     PEGMILDFFQSVYDAGSSLAGWDRPRLEMERVP"
     gene            1246..1515
                     /locus_tag="VGR29_05080"
     CDS             1246..1515
                     /locus_tag="VGR29_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEV2072997.1"
                     /translation="MAQQCTHLDPIHEVTPSADGCEECLQMGDTWVHLRLCLICGHVG
                     CCDSSKNKHASKHFHAVGHPLVRSLERGEDWMWCYEDRVMMASPR"
     gene            complement(1565..1996)
                     /locus_tag="VGR29_05085"
     CDS             complement(1565..1996)
                     /locus_tag="VGR29_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2072998.1"
                     /translation="MNAQLHFITLATEDLDAARTFYRDGLGWEPLLDVPGEIIFFQVA
                     PGLTLGFFDAEKFAEDLNRPVATTGVSGVTLSHNVGNREAVATTIDRLASLGATVLKA
                     PQEGAFGGIYHGHVRDPNGIVWEIAHNLGWLIDEAGNVLFG"
     gene            2206..2826
                     /locus_tag="VGR29_05090"
     CDS             2206..2826
                     /locus_tag="VGR29_05090"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV2072999.1"
                     /translation="MDDIHFYEPSTGHGLPHDPFKAIVAPRPIGWISTLSTSGIPNLA
                     PYSFFNAICDTPPIIAFSSSGPKDSLTNAEATGEFVWNMATRALAEAMNASSAPVPPE
                     IDEFMLARLETAPSRIVAPPRVAASPASLECRLLQIVALHDLDGQPAAYQLVIGQVVG
                     VHIDRAFLHDGLFDTGAAHPIMRAGYRDEYVEATPETMFRMMRPGA"
     gene            3149..4477
                     /locus_tag="VGR29_05095"
     CDS             3149..4477
                     /locus_tag="VGR29_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEV2073000.1"
                     /translation="MDETTVVTIWFVIIGFLLVVMAVGRTLLRPLPLSTPLLYLIVGI
                     VFGEAGFELLSIDPFNNAALLERVTEIAVIVSLFTAGLKLRVPLRDAAWRLPLSLASL
                     SMVVTVGLIALTGYYGLGLTLGAAVLFGAVLAPTDPVLASDVQVTEPGDRDHLRFALT
                     GEAGLNDGTAFPFLMLGLGLMGHHELGEKWSRWWLVDVAWAIPGGLVIGFSLGTAIGM
                     LVLYLRRRYPSAMGGEDFLAMGLIALSYGLALLLHTYGFLAVFAAGLAMRKIEEQAVG
                     HDRSPQEVLEEVATDDLEERAATDETLAPAVMTRSLLHFNEQLERVGEVGIMLLVGAM
                     ISTRYLPVVALWFVPLLFLVIRPIAAAIGVLGSDTTRVQRGFISWFGVRGIGSIYYLM
                     FAVVHGLEEATARELIGLTLTVIATSVVIHGISVTPMMSWYNQHYRPEER"
     gene            4842..6644
                     /locus_tag="VGR29_05100"
     CDS             4842..6644
                     /locus_tag="VGR29_05100"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEV2073001.1"
                     /translation="MARSLSGGNLASMVARGFGSPLATLREFETSHVLDAGSRPSHSA
                     GPQAIQRLRRLFFRWFLPAAMALLFLLAIGLVVFRLAYEDRLYPAIVVGDVAVGGLTP
                     AEAQTRITERAASLENGTVSFSFGGQTWTPTLSELGVTVLLDESIEEALWLGREGSSA
                     SRLAFMGEILDADQVVPLQTQINQTILNAWFDSVDRDLGQLPVNAQIVIDGTSVSVTP
                     GTDGMGVDREAATTHLVHALSTLQPLAVSLPTAVLHPDITEEDLVSVRAEVEQVISKP
                     VPITFEDQQWTIEGPTLAPFLHAETVMGSGGPTARLHVDTKALARELRTRFSPLVNRK
                     PIDAVLGWNNGLVVIEPSTTGAVVKSNDFADAVEQSVLNGHTSVTVPVVTVDPEIDGG
                     QLDAYGINTLLGQGDSNFDGGNEGRDENVRVSTEFLNGTLVPPRGTFSFNEAIGEITY
                     EKGYKDALVATAEMVGPGVGGGVCQLSTTVFRAAIYSGMSIKEWYPHTNRLPDYERDG
                     WGPGFDASILQVGPTMDDWADFKFENNTDNWLLIEATISYPYVYVNIYGSDDGRSVDT
                     NAYEMGDNAFAFTRVIYDAQGTVIEERTFESPFQ"
     gene            complement(7107..8075)
                     /locus_tag="VGR29_05105"
     CDS             complement(7107..8075)
                     /locus_tag="VGR29_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEV2073002.1"
                     /translation="MRPFAYTRPESIEEALTLLDGETRPLAGGTDLLTLMKGEIVTPG
                     HLVDIKRLPELSHDIAQADGAISLGSLTTLSQIEHDPLIREHLPALGQAASLAATPQL
                     RNMATIGGNVLQRPRCWYYRNDEVTCWLQGGDECYARGGDNRYHAIFDVSPCVAVHPS
                     DMASVLVAYDATIQYRDAGEHEIGVEAFFQPPTPERRTENVLPENAVITGITIPTPSS
                     SNRSVYLKAMDRKVWAFALVGVAISLEMESDTVAKANVVLSGVAPTPVRSSQAEQLLV
                     GSRLDAEVVALAADAAVANAEPLQHNGYKVPLVTALIRQALQEAVG"
     gene            complement(8072..>10325)
                     /locus_tag="VGR29_05110"
     CDS             complement(8072..>10325)
                     /locus_tag="VGR29_05110"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV2073003.1"
                     /translation="WDAATSLDIVGKGTSRLEAFEKVTGHAQYSSDVRLTRQSYAKVL
                     RSPHPYARVTSIDIAGAEAMLGVHAIIHAGNIENIEWYNGSRLFDPTVRLIGDEVAAV
                     AAESEEVAEDALRRIRVTYEVLPHVTSLDGTKAPDAPQVRSDAEGEGNTAGEPKEYER
                     GDVEAGLRQAEVLITRTYTTQAAVHNSLEPHGCTASWEDDHLTLWVSTQSIFTVRQQV
                     AEALGMPETKVRVIKHHMGGGFGAKQIAWKPEVMAALLSRRAGRPVQLMVDREGENLV
                     VGNRPASRQRLRLGARRDGTLTAIDVDIEQTIGAYMTGGEASNVSGMYQTLYRCENVR
                     TVQTPFYVNAGPGVAFRAPGHVESAWALEQAMDELARILDLDPIELRMRNYSETEQLE
                     NKPYTLPEGLRLSYDRVRQAAGWDERRSSSNTGRVRRGFGFAAHDWIGGSGSPPGYAW
                     IELNEDGTADVVTGTQDIGTGTRTGLAQVAAEELGLPLDDVSFHLGDTATGPYSPVSS
                     GSATQATIGPAIRAAAIDVKAQLLQVASAAMEMPANRLSVRQGKVMVDGDPKRAMTVK
                     DVMQEVAPHTLRAQGARGTNPEDKAARTFGSQIVEVEVDTETGEVTVKRVVASHECGR
                     VVNPMMVESQVIGAVTQGLGFALMEERIIDHRLGMVMNPNLEDYKIPTIADIPEIINV
                     PVNVPDPDANPTGAKGIGEPPLIPTAPAIANAIHDAVGIRVLDAPISRQRMLELLHAQ
                     HMTSGQEQPS"
BASE COUNT         1876 a         3388 c         3018 g         2043 t
ORIGIN      
        1 ggcttaacaa ttccactatc tcgatgcgct aagatcagaa acatcgtggt tacaccagat
       61 acgtccagtt gtcggacccg atccaggcac cgcccccatg cattccgcga tcgcatgcga
      121 tgcgtgcgcg gcttcacgca ctgccgcacc caatgagccg tgattcgggt gcgtcccagc
      181 catacggcag cgagccgtaa cgccatggac cgtgcgccat aaaaaagaag ggaagtcgga
      241 taccgatgac actccagcac caggggaccc agtggccgac ccttccctat gatgaatggc
      301 aagcaaccca ggcaaccctc catcgatgga tgcagatcgt gggcaaggtg acgctcgagg
      361 gcacaccgtt cctcaacgag tggtggaacg tcgggctcac ggtgacgtcc cggggcatga
      421 ccaccccctt gatcccgtat ggccaggagg cgttcaccat cgcctttgac ttcatcgatc
      481 acacgctctc catcgatgta accgatgggc ggaccgggag actaccgctg cgtcctcagt
      541 cggtcgccag cttctacctt gaattcttca acacgctcga agcgctcggg atcgacatgc
      601 cgatcaatcc gatgcccgtc gagatcgtcg acccggtttc cctcgatttc gatgagggca
      661 acgacgccta cgacgctgat gcagtctatc gctggtggcg catcctgttc cagaccgaaa
      721 aggtcctcca gcggtaccgt tcatccctcg ccggcaaaag cagccctgtc ctcttcttct
      781 ggggatcgtt cgatctcgcc gccacccgct tctcggggca ccctgcatca ctcccgccag
      841 gtgcgccgcg cttcatgcaa ctcgccgagg atcaggagaa catcgctgtc ggcttctggc
      901 cggggaacga caactacgcc ggtgtccctc ttggggagcc ggccttctac gcgtacatct
      961 tccctgaacc cccgggcttc aaggaggcga cgatacgtcc ggcggaagcc gcctaccgcc
     1021 ctgagctggg ccagttcctg ctcccctacg aggcagtccg gcaggcaccg gcaccagaag
     1081 ggatgatcct cgactttttc caaagtgtgt acgatgctgg ctcatcgctc gccggctggg
     1141 atcggccgag actggagatg gaacgagttc cctgagacgt acgatgcggc acctgatgcc
     1201 gctcactctt catctcgctg ccacaatgct gaacggagta tctacatggc acaacaatgc
     1261 acccacctcg acccgattca cgaagtcacc ccgagtgccg atggctgcga ggaatgcctg
     1321 cagatgggcg atacatgggt tcacctgcgg ctctgcctga tctgtggcca tgttggttgc
     1381 tgtgattctt cgaagaacaa acacgcctcg aaacacttcc atgcggtcgg ccaccctctt
     1441 gtccgctcat tagagcgagg cgaggactgg atgtggtgct acgaggatcg tgtcatgatg
     1501 gcatcgccgc ggtaacgata gcgtggatgc ccggaatcac ctccaaaacc cgattcgggt
     1561 aacctcatcc gaagagcaca ttccccgctt catcgattag ccaaccaagg ttgtgcgcga
     1621 tctcccagac gatgccgttt ggatccctga catgtccgtg atagataccc ccgaacgcac
     1681 cctcctgcgg tgccttcagg acagtggcgc caagagaggc cagcctgtcg atggtcgtcg
     1741 cgaccgcttc gcggttgccc acgttgtgtg agagtgtgac tcctgaaacc cctgtggtcg
     1801 cgacggggcg gttcaggtcc tcggcgaact tttccgcgtc gaagaaaccg agggtaagtc
     1861 cgggcgcgac ctggaagaag atgatctctc cgggcacgtc cagcagtggt tcccatccca
     1921 gcccgtccct gtagaaggta cgcgccgcgt caagatcctc agtggccaac gtgatgaaat
     1981 ggagctgggc attcacgggg cccttccctt cctaccaagc agcagccagc cctgctgccc
     2041 ctgttccttg tgcggcacca tgccggcgat tttcccatct atcgattcac cgtagcgacc
     2101 gggcagcatc ccaagcctga aagccccaat gattcacggc cttccactct gcacccatgt
     2161 cgacaaccgt tgtgccacaa cccatcttcg gatatgctcc tcgccatgga cgatatccac
     2221 ttctacgagc cctccaccgg ccacggcctg ccgcacgacc cattcaaggc aatcgtcgct
     2281 ccacgcccga tcgggtggat ctcgacgctg agcaccagcg ggatccccaa cctcgcgccc
     2341 tacagcttct tcaacgcgat atgcgacacg ccgccgatta ttgccttttc atcgagcggc
     2401 cccaaggata gcctcaccaa tgctgaggca accggcgagt tcgtctggaa catggcgacc
     2461 cgcgcgctgg ctgaggcgat gaacgcctcc tccgcccccg tgccaccgga gatcgacgag
     2521 ttcatgctcg ccaggctcga gactgccccc agccgcattg tcgcgccgcc gcgcgtcgcg
     2581 gcaagcccgg cgtcgctcga atgccggctg ctgcagatcg tcgcgctgca cgatctcgac
     2641 ggccagccgg ccgcctacca gcttgtgatc ggccaggttg tcggcgtcca tatcgatcgc
     2701 gctttcctgc acgatggcct cttcgatacc ggcgcagcgc acccgatcat gcgcgccggt
     2761 tatcgtgatg aatatgtgga agcgaccccg gaaacgatgt tcaggatgat gaggcccggc
     2821 gcataatccg gaccgcacgc gtcacacttc gaaagtaggc tggtcgttcc gaagaccacc
     2881 ggtgctggtg ccctccagga ccacatcact gaaccaggat ccatgcaccg ataacccgcg
     2941 gtgccacagg atcaggcaga tcactcaagg cgacagcgag cccgccaagc cccaccgaga
     3001 tcttcgcact cgtagaaata ctcgaccgat gaactatcgg ccgggatttt gcgatgtatc
     3061 cggggccggg atgatactcg gtgcaaaggt gattcagcga tgttgacacc atacgagcag
     3121 tggatagccg ccgcctccag gagatgtgat ggacgaaacg acggtggtta cgatctggtt
     3181 tgtcataatc ggattcttgc tggtagtcat ggcggtcggg cgtacgctgc tcaggccgct
     3241 tcctctttct accccgctcc tctatctcat cgtcgggatc gtcttcggcg aggcggggtt
     3301 cgagctgctc tcgatcgacc ccttcaacaa tgccgctctg ctggagcgcg tgactgagat
     3361 cgccgtcatc gtctcgctct tcaccgcggg cctgaagctc cgagtgcctt tgcgtgatgc
     3421 cgcctggcga ctgccgctgt cgctcgcgtc cctgtctatg gtggtcaccg tgggcctgat
     3481 tgccctgacg gggtactacg gtttgggctt aaccctcggc gccgcggtgc tctttggggc
     3541 ggtgcttgct cccacggacc ccgtacttgc ctcggatgtt caggtcacgg agccgggtga
     3601 ccgcgatcat cttcgcttcg cgctcaccgg cgaggcaggg ctcaacgacg gcaccgcctt
     3661 ccccttcctc atgctcggcc tcggcctgat gggtcaccat gagctcggcg agaaatggtc
     3721 gcgctggtgg ctggtcgatg tcgcctgggc aatcccgggc gggttggtaa tcggcttttc
     3781 cctcgggacg gcgatcggca tgctcgtcct ctacctgcgg cggaggtacc cgagcgccat
     3841 gggtggtgag gactttctcg ccatgggcct gatcgccctc tcctacggcc tggcgctcct
     3901 gctgcacacc tacgggtttc tcgcggtatt tgcggcgggg cttgccatgc gaaaaatcga
     3961 ggagcaggca gtggggcacg atcgctcgcc gcaggaggtg ctggaggaag tcgccacgga
     4021 tgatttggag gaacgggcag cgaccgacga gacgctcgct ccggcggtga tgacacggtc
     4081 cctgctgcat ttcaacgagc aactggagcg ggttggcgag gtcgggatta tgctgctggt
     4141 gggggcaatg atctcgacgc gctacctccc ggtcgtggcg ctctggttcg tgccgcttct
     4201 cttcctggtg attcgaccca tcgcagcggc gattggggtg ctcgggtcgg acaccacgcg
     4261 ggtgcagcgg gggttcatca gctggttcgg ggtccgcggg atcggctcga tctattacct
     4321 gatgtttgcg gtcgtgcacg ggctggagga ggcaacggca cgggagctga tcgggctgac
     4381 actgacggtc atcgccacct cggtcgtcat ccacggcatt tcagtgacac cgatgatgtc
     4441 ctggtacaac cagcactata ggcccgaaga gcgataaatc acatcattcc tctatccgca
     4501 tgacactccc atagtcatgg actatctcat gttggcccag gaaacggtga gcgatagagg
     4561 ggtcagggat cgttgcagaa gctgccccac cgcgcctgcc gatagtgaca aactgacaac
     4621 gcctgacggt tttgggtggt atattggtga ggcgaaaaga tctccgaggg agccatggtt
     4681 tcaggacgat ccatggagga cgggaggtcg taggtcaggc agacattcca gccagctggg
     4741 atgtcatctt taggctctat gcgctggccg tgcaacctga atactcactg gcgcgcgtag
     4801 gtggggcgtg cgtgtgccta acggaatgag agaaccgaag gatggctcgg tcattatccg
     4861 gtgggaacct ggcgagcatg gtggcacgcg ggttcggttc ccccttggca acgctacgtg
     4921 agttcgagac cagccacgtg ctggatgcag gcagcagacc ctcccacagt gccggtcccc
     4981 aggcaataca gcggctccga cgactctttt tccgctggtt tctcccggcg gcaatggcgt
     5041 tgctcttcct gctcgcgatt gggctcgtcg tctttcggct cgcctacgag gatcgcctct
     5101 atcccgcgat cgtcgtaggt gacgtcgccg tcggtgggct cacaccagcc gaggcgcaaa
     5161 ctcgtatcac cgagcgcgca gcttctctgg agaacggcac ggtttccttc agcttcggcg
     5221 gtcagacctg gacgccaaca ctgtcggagc tgggtgtcac agtccttctg gatgaatcca
     5281 tcgaagaggc cctgtggctc gggcgagaag gctcatccgc ctcccgcctg gcgttcatgg
     5341 gagagattct ggatgcggac caggtagtcc cgctacagac acagatcaac cagaccatcc
     5401 tgaacgcttg gttcgactcg gttgatcgag acctcggtca acttcctgtc aatgcccaga
     5461 tcgtcatcga cggaaccagc gtctctgtga cgcctggcac cgacggcatg ggggtcgacc
     5521 gtgaagcggc caccacccac cttgtccacg cattgtcgac actccaacca ctggccgttt
     5581 cgcttccaac tgcagtcctc caccctgata tcaccgagga ggatcttgta tcggtccgcg
     5641 cagaggtcga acaagtaatc agtaaaccgg taccaatcac gttcgaagat cagcagtgga
     5701 cgatagaggg cccaacgctg gctccctttc tccacgctga gaccgtgatg ggatccgggg
     5761 gacccactgc ccggctccac gtcgatacca aagcactggc acgcgagctc cgcacgcggt
     5821 tctcgcccct ggtgaaccgc aagccgatcg atgccgtact tggttggaac aacggtctcg
     5881 tcgtgattga accgagcacg accggtgcgg tggtgaaatc aaacgatttc gccgatgccg
     5941 tagagcagag cgtcctcaat ggccacacga gcgtgacagt cccggttgtg accgtggacc
     6001 cagagatcga tggtggacaa cttgacgcct atggcatcaa caccctgcta ggacaaggag
     6061 attccaactt cgatggtggg aacgagggac gagacgaaaa cgtacgagta agcaccgaat
     6121 ttctcaacgg tactctcgtc cccccgcgcg gcaccttttc gttcaacgag gccataggcg
     6181 agatcacgta tgagaagggc tacaaggatg cacttgtggc aacggcggaa atggttggcc
     6241 caggtgtggg cggtggtgtc tgccagcttt cgaccaccgt ctttcgcgcc gccatctatt
     6301 ccggcatgtc gatcaaggaa tggtacccac acaccaatcg gcttcccgac tacgagcgtg
     6361 atggatgggg accggggttc gatgcgtcga tccttcaggt aggcccgacg atggatgatt
     6421 gggcagactt caagttcgag aacaacaccg acaactggct tctcattgaa gcgacgatct
     6481 cctaccccta tgtctacgtc aacatctacg gttccgatga cggccgatca gtggacacga
     6541 acgcctatga gatgggcgac aacgccttcg cctttacccg cgtcatctac gatgcccaag
     6601 gcacggtgat cgaggagcgc acgttcgagt ctcccttcca gtagagcgcc gcaggcgaaa
     6661 aatcccgtga tgctgcccga caacggtgat gggaatacct tcccttgatc atgtctgagg
     6721 gacgttctag ggctattttc agagaggttg caggtctgta gcggaggact tacggtgcag
     6781 gcagcccgtt cgcggccacg atgcagcact agcctgccct gaacctgtcg aacaggtcct
     6841 cctggcagga cgcaggggac gacgttgctg cggcgacggg ctgctacacc ctcccctggt
     6901 ggatcaatct cattgcaaat cgctctaggg gatagcttct ctagatcgtg cctgagggct
     6961 gtccaaatag cacgtagcct ggacagccct caggaacatc tccggccggt tgcacacgcg
     7021 tcgatttctg ccacctgggc caccaactgg ccatcctggt gcgcatccct cgacacttat
     7081 gatcgccaga tgagctcgtc cggctcctac ccaaccgctt cctgcaatgc ctgccggatg
     7141 agcgctgtca ccagtggcac cttgtacccg ttatgctgca gcggttccgc gttcgcgacc
     7201 gccgcgtccg ctgccaaggc aaccacctcc gcatccagcc tcgacccaac cagcagctgc
     7261 tccgcctgcg acgaccgcac cggcgtcggc gcgacaccgc tcaacacgac gttcgccttc
     7321 gcgaccgtgt cgctctccat ctcgagcgag atggccacac cgaccagggc gaacgcccac
     7381 accttccgat ccatcgcctt caggtagacg ctccggttac tcgaagacgg cgtcgggatg
     7441 gtgatcccgg tgatcaccgc gttctccggc agaacgttct cggtccggcg ctccggggtg
     7501 ggcggctgga agaatgcttc aacaccgatc tcatgctccc cagcgtcccg gtactggatc
     7561 gtcgcgtcat aggccacgag caccgatgcc atatcggagg ggtggaccgc cacgcagggg
     7621 ctcacatcga agatcgcgtg ataccggttg tcgcctccgc gcgcgtaaca ctcatcgcca
     7681 ccctggagcc agcaggtcac ctcatcgttg cggtaatacc agcagcgcgg ccgttgcagc
     7741 acattgccgc cgatcgtcgc catgttcctc agctggggcg ttgccgccaa cgaggccgcc
     7801 tgccccagtg ccggcaggtg ctcgcggatc aacgggtcat gctctatctg cgacagcgtg
     7861 gtcagcgatc caagtgagat cgctccatcg gcctgggcaa tgtcgtgcga aagctccggc
     7921 aaccgcttga tatcgaccag atgccctggc gtgacgatct cgcccttcat cagcgtcagc
     7981 aggtcggtgc cccctgccaa cggccgggtc tccccgtcca ggagcgtcag ggcctcctca
     8041 atactctcgg gccgggtata ggcaaagggt ctcatgaggg ttgctcctgg ccggaggtca
     8101 tgtgctgcgc gtgcagcagc tcaagcatcc gctgtcgcga aatcggcgcg tcgagcacgc
     8161 gaatgccaac ggcgtcatgg atggcgttgg cgatcgccgg cgcggtcggg ataagcggtg
     8221 gctcaccgat gcccttcgcc ccggtcgggt tcgcatccgg gtcgggcaca ttgaccggca
     8281 cgttgatgat ctcggggatg tccgcgatgg tgggtatctt gtagtcctcg aggttggggt
     8341 tcatcaccat accgagccgg tgatcgatga tccgctcctc catcaaggcg aaaccgagcc
     8401 cctgcgtcac cgccccgatc acctggctct caaccatcat cgggttgacc acgcgcccgc
     8461 attcgtgcga ggcgaccacg cgtttcaccg tcacctcacc ggtctcggtg tcaacctcca
     8521 cctcgacgat ctgggagccg aacgtccgcg ccgccttatc ctcggggttc gtccccctcg
     8581 caccctgggc gcgcagcgta tggggtgcga cctcctgcat cacgtccttc accgtcatcg
     8641 cgcgcttcgg gtcgccatcc accatcacct tgccctgccg caccgagagg cggttcgcgg
     8701 gcatctccat cgccgcggat gctacctgca acagctgcgc cttgacgtcg atcgccgctg
     8761 cccggatggc aggcccgatc gtcgcctgcg tcgcgctacc ggagcttacg ggtgagtaag
     8821 gccccgtggc cgtgtcgccg agatggaatg acacgtcgtc caacggtaac cccagctctt
     8881 ccgcggcgac ctgggccagc cctgtccgtg tacctgtgcc gatatcctgg gtgcctgtca
     8941 ccacatcggc cgtgccgtcc tcattgagct cgatccatgc ataccccgga gggctgccgc
     9001 tgccacctat ccagtcgtgc gcggcgaacc cgaagcccct ccgcaccctt ccggtgttgg
     9061 aggaggaccg tctctcatcc cagcccgccg cctggcgcac ccggtcgtag ctaaggcgca
     9121 agccttcggg gagggtatat ggcttgttct cgagctgctc tgtctcactg tagttccgca
     9181 tccgcagctc gatcggatcg aggtccaaaa tgcgcgccag ttcatccatc gcctgctcga
     9241 gcgcccaggc ggactcaaca tgccccggtg cccggaaggc gacacccggc ccggcattga
     9301 catagaacgg cgtctgtacc gtgcggacgt tctcgcagcg atagagtgtc tggtacatgc
     9361 cgctgacgtt gctcgcctcg ccgccggtca tgtaggcgcc aatggtctgc tcgatatcga
     9421 catcgatcgc cgttagcgtg ccgtctctcc tcgccccaag ccgcagccgt tggcgggaag
     9481 ctggccggtt tccgaccacc aggttctctc cttcgcgatc caccatgagc tgcaccggac
     9541 gtccggccct cctcgacagc agtgccgcca tgacctccgg cttccaggcg atctgctttg
     9601 ccccgaagcc accgcccata tgatgcttga tcacccgcac cttcgtctcg ggcatcccga
     9661 gcgcctcggc gacctgctgc cgaaccgtga agatcgactg ggtcgacacc cacaatgtca
     9721 ggtgatcgtc ctcccaagat gccgtacacc cgtgcggctc caaactgtta tggacggccg
     9781 cctgcgttgt gtaggtcctc gtgatgagga cttccgcctg ccgcaatccg gcctcgacat
     9841 cgccgcgctc gtactccttc ggctcccccg cggtgttccc ctcaccttcg gcatcgctcc
     9901 ggacctgcgg cgcatctggc gccttcgtgc catcaagcga ggtcacgtgc ggcagcacct
     9961 cataggtcac gcggatgcgg cgcagcgcat cctccgcgac ctcctccgat tctgcggcga
    10021 ccgcggcgac ctcatcgccg atcagccgca ccgtcggatc aaagagccgg ctcccgttgt
    10081 accactcgat gttttcgatg ttgccggcgt ggataatggc atggacgccc agcatcgctt
    10141 ccgccccggc gatatcgatc gaggtaaccc gtgcatacgg gtgggggctc cgcagcacct
    10201 tcgcgtagct ctgtcgcgtc agtcgcacgt cggagctgta ctgggcgtgg ccagtcacct
    10261 tctcgaacgc ttccagccgg gaggttccct tgccaacaat atcgaggctg gtggccgcgt
    10321 cccac
//
LOCUS       DASYWM010000123         6012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_267242, whole genome shotgun sequence.
ACCESSION   DASYWM010000123 DASYWM010000000
VERSION     DASYWM010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267242"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VGR29_05115"
     CDS             complement(<1..190)
                     /locus_tag="VGR29_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2073004.1"
                     /translation="MVSQENTLQPLLDVKHLTKTFGSRDNEVRAVDDVSFTVTQGGII
                     SIVGESGSGKSTLARMLLR"
     gene            complement(305..1183)
                     /locus_tag="VGR29_05120"
     CDS             complement(305..1183)
                     /locus_tag="VGR29_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2073005.1"
                     /translation="MEVRDVRAYYQIRHGAPIKAVSDVSFNLVHGEILGIVGESGSGK
                     STLASILSSNAAPNLKIQSGLLTLDDRETIDIAKARGTPRDWRGKVVSLLPQRALNSL
                     NPTARIGDYAFDVMRAHNSRLHRREALELAGGRLEELGLPSRVLHNYPHQLSGGMRQR
                     VVTVIATLLNPTVLIADEPTSALDVSSQKALILMLQQMMSEGMISRIIFITHDLPLLS
                     TIASRIAVMYAGEIVEIGTTRQVVLQPKHEYSKTLIGSTLALDEGLRQRTNKGRQGSS
                     PETRYPPHARKVPSAL"
     gene            complement(1195..2103)
                     /locus_tag="VGR29_05125"
     CDS             complement(1195..2103)
                     /locus_tag="VGR29_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073006.1"
                     /translation="MSTLPATAPDAAIDPDAKSSIKGRLRSLPSQFSGRFWFALALTI
                     GVVLFAFLGPFLTSIENPDRIVGGLYLEPSAEAWLGTDNFGKDVFTQLMYGTRTSLII
                     GVISGLIATLIGVIIGISAGFYGGIIEEVMMGITNVVITIPSIVVLILLSIAIGSRSI
                     YSIAIIIGVTSWPWTARAVRAQASSVRTREHVDLARLTGANTFSLMIWEVLPYMLSYI
                     VMAFVLQLTSGILQEAALSMLGLGPSNSISLGIMLFWALLWESIRTGAWWAFVPPTIF
                     LTLIAFTLLLLQSSLDEVFNPRLRRS"
     gene            complement(2113..3255)
                     /locus_tag="VGR29_05130"
     CDS             complement(2113..3255)
                     /locus_tag="VGR29_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073007.1"
                     /translation="MLMKGTGQFSPVPFGTPAQDRPFARRFHRAREETDDGHRTIGDS
                     TMTQFQRYILRKAAWFLAAFLAALVLNFYLPRLVPGNPVDSIVSQMSQGGADAASMAA
                     IYETYISEFGLDEPLLLQFFTYAGNVLRGDLGTSFALYPAEVTGLIADALPWTLALQL
                     PAILVGWILGNALGAIAAYKGGWFDRGGFLASLLLSSMPYYAFAILLLYLVAVRWDVL
                     PAAGGYSFGTEPSFTWSFVSNAAEHYLLPFLSLVLIFIGGQAIGMRSMAIYELGADYV
                     DFSRGLGISDSRIVRYIFRNAMLPQITGLALSIGSLVGGALITEVVFSYPGIGSLLFT
                     AIRQNDYPVIQAITLLIMVAVLLANFLVDIAYGVIDPRIRSAQTGT"
     gene            complement(3265..5010)
                     /locus_tag="VGR29_05135"
     CDS             complement(3265..5010)
                     /locus_tag="VGR29_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2073008.1"
                     /translation="MSKGYLSSRRDIIKHGAALGSMAAVGAASFTRYAPGASAAQLAR
                     SETLYVAGMQWGPPTTFNPLSANAPWPVDTYFVHIYETLYTFNVVTGEIEPLLAADLP
                     EETDEGVTITLHEGTAFQDGEPLTAEDVVYSITLPQRQEGIPYSTVTDYVSEVEAVDE
                     RTVRLTYNPERRHPGMVRHLLTQIHVLPQHIWEEREAEGVASVVDMEPVGSGPYTLSI
                     SSAEQVVITRYEDYWGADVVGMPVPKNIVHPIFDSNDAGNLALQRGEVDLSQQFAPQI
                     WQMWLERDLPVGTWYDDAPYHVPGSIPMMHINVSRPGLDNPAVRRALAYSINYPQIAE
                     TAMSRYSVPVNPSLLIPQGAEEEFFRADELAETGGWEYNPDVARQILEEELGATLEDG
                     VYVLEDGTRLGPWTVQCPYGWTDWMTALELVAQGAQEVGFDITTQFPEAPVVTTAVQN
                     GDFDLVLWGVAGTSAAGPWLRFRDVLDNRGVPDFGQTAFWNYNRFNNDEAEALLDQAA
                     TAEGEELVELYSQLDAIFRENVPTIPLMYRPLQFYEFNESVWTNFPTEENPYAPPMHY
                     GSGIKILYGLEPAGE"
     gene            5399..>6012
                     /locus_tag="VGR29_05140"
     CDS             5399..>6012
                     /locus_tag="VGR29_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2073009.1"
                     /translation="MSLPQAPSGDAKQGTPDAPQIEGTLPAALTPLIGRQEEITRISR
                     VLRERAARLLVLTGPGGVGKTRLALAIAEATSDAYQDGVALVNLGPVITSDGVLPAIS
                     AVLGVREASQDHLYAAVRESLNNREMLLVLDNFEQVVEAAPILTDLLTVSPALTILVT
                     SRSVLGVYGEFVFPVPPMKVPATTESSMETIESSEAVQLFVNRVR"
BASE COUNT         1278 a         1793 c         1793 g         1148 t
ORIGIN      
        1 gacgcagcag catacgggca agcgttgatt tgccgctgcc actttcgccg acgatcgaga
       61 tgatcccgcc ttgggtaaca gtgaacgaca catcatctac cgcccgcacc tcgttgtcac
      121 gtgagccaaa ggtcttggtc agatgcttca cgtcaaggag cggttgcagt gtgttctcct
      181 gtgacaccat tctctccctc aggctcgtcg tgcccatcca cctgttactt cacccagcag
      241 gctgcgtcat ggtgctctga gccgatcttt aggggtagct tgcgagaaaa cgcccatcgc
      301 aacttcacaa agccgatgga accttgcgag catgcggcgg atacctcgtc tcaggagaac
      361 tcccctgccg tcccttattt gtgcgttgac gcaatccttc atccagtgca agggtggagc
      421 caatgagcgt cttggaatac tcatgtttcg gttgtagtac cacttgccgg gtcgtgccta
      481 tttccacaat ctcacccgca tacatcacag cgatgcgaga tgcgatggtg ctgagcaatg
      541 gcaagtcatg ggtaatgaag atgatccggg agatcatccc ctcgctcatc atctgttgga
      601 gcatcaggat cagtgctttc tgggatgaaa catccagagc ggatgtcggc tcatcggcga
      661 tcagcacggt cgggttgagc aacgttgcaa tgacagtcac tacacgctgg cgcatgccac
      721 cactgagctg atgaggatag ttgtggagca cgcgcgaggg gagaccgagc tcctccagtc
      781 gtccacccgc caactccagc gcttcacgac ggtggagcct ggagttatgc gctcgcatca
      841 catcgaaggc gtagtcaccg atccgcgcgg taggattaag tgagttcagc gcgcgctggg
      901 gcagcaagga aacgaccttg ccacgccaat cgcgcggcgt tccccgagcc ttggcgatat
      961 cgatcgtctc gcgatcatcg agcgtcaaca gcccgctctg gatcttgagg ttgggggcgg
     1021 cgttgctgga gagtatcgac gcgagcgtag attttccaga cccggattca ccgacgattc
     1081 ccaaaatctc accatgcacg agattaaagg agacatcgct gaccgccttg atcggcgccc
     1141 cgtgtcggat ttgataataa gcgcgtacgt cacggacttc cataaacttt atccctaact
     1201 tcggcggagg cgcggattga agacttcatc gagactggat tgcaacaaca gcagcgtgaa
     1261 cgcaatcaac gtcaggaaga tcgtcggcgg cacgaacgcc caccaggcgc ccgtccgaat
     1321 ggactcccac agcagcgccc agaagagcat gatgccgagt gagatcgaat tcgacggtcc
     1381 gagtccgagc atgctgaggg ctgcctcctg caagatgccg ctggttagct gcagcacgaa
     1441 ggccatcacg atgtaggaaa gcatgtacgg cagcacctcc catatcatca gcgagaaggt
     1501 attcgcgccg gtaagccgcg cgaggtcgac atgctcccgg gtgcgcacac tgctcgcctg
     1561 cgcccgaacc gcacgggcgg tccacggcca actcgtgacg ccgatgatga tggcaatgga
     1621 atagatcgag cgagaaccga tagcaatcga tagcaaaatg agcacgacga ttgagggaat
     1681 ggtaatcacc acgttggtaa tgcccatcat tacctcttcg attatgcctc cataaaaccc
     1741 tgcggagatc ccgatgatca cgccgatgag cgtcgcaatc agtcccgaga tgacgccgat
     1801 gatgagcgaa gtccgggtgc cgtacatgag ctgcgtgaag acatccttgc cgaagttgtc
     1861 agtgcccaac cacgcctccg ccgacggctc caggtagagc ccgcccacga tgcgatcagg
     1921 attttcaatg ctggtcagga aggggcccag gaaggcgaag agcacgacgc cgatcgtgag
     1981 cgccagcgca aaccagaagc gacccgagaa ctggctcggc agtgagcgca atcgcccctt
     2041 gatcgagctc ttcgcgtcgg ggtcgatggc ggcatcgggt gccgttgcag ggagtgtgct
     2101 catggcggtc tcctaggtcc cggtctgtgc agagcgaatc cgcgggtcaa tgacaccata
     2161 cgcaatgtcg acgaggaaat tcgccaggag cactgctacc atgatcagca gcgtgattgc
     2221 ctggatcacc ggatagtcgt tctggcggat ggcggtgaac aggaggctgc cgatgccggg
     2281 gtacgaaaag accacttcgg tgatcaatgc acctcccaca agcgacccga tcgagagcgc
     2341 gaggccggtg atctgcggca gcatcgcgtt gcggaagata taccggacga tgcggctgtc
     2401 agagataccc aaaccgcgac tgaaatctac atagtcggca ccgagctcgt agatagccat
     2461 cgaacgcatg ccgatcgcct gtccaccaat aaagatcagc acgagtgaga ggaacggcag
     2521 caagtagtgc tcggcggcgt tggagacaaa cgaccaggta aaactcggct cggtaccaaa
     2581 agaataccct ccagcagccg gcagcacatc ccagcggacc gcgaccaggt agagcaacag
     2641 gatcgcaaag gcgtagtacg gcatgctcga aaggagcagg gatgccagga acccaccccg
     2701 atcgaaccag ccgcccttgt aggcagcgat agcgccgagc gcattgccaa ggatccaacc
     2761 gaccaggatc gcgggtagct gcagcgctaa cgtccacggc aaggcatcgg caattaatcc
     2821 ggtgacctct gcgggataga gggcaaatga ggtgcccaga tcaccgcgca gcacattccc
     2881 tgcataggtg aagaactgca gaaggagcgg ctcgtcaagc ccgaattcgc tgatgtacgt
     2941 ttcatagatg gctgccatcg acgccgcatc ggcgcccccc tgggacatct gcgagacgat
     3001 gctatcgacg ggattgccgg gaacaagccg gggcagatag aagttgagca ccagcgccgc
     3061 aagaaatgcg gcgaggaacc aggcggcctt tcgcaggata tagcgttgga attgcgtcat
     3121 cgtgctgtcc ccgatggtgc ggtgtccatc gtccgtctct tcccgggcgc ggtggaagcg
     3181 acgggcgaat ggccggtcct gcgccggtgt gccgaagggg accgggctaa actgccctgt
     3241 ccccttcatc agcatggtct tggattattc ccccgccggc tcaaggccgt agagaatctt
     3301 tatgcccgaa ccgtagtgca tcggcggagc gtaggggttc tcctcggtcg ggaagttcgt
     3361 ccagaccgat tcgttgaatt cgtagaactg gagcgggcgg tacatcagcg gaatggtcgg
     3421 cacgttctcg cggaagatcg cgtccagttg agaatagagc tcgactagct cttcaccctc
     3481 ggccgtcgcc gcctggtcaa gtagcgcttc cgcctcgtcg ttgttgaagc ggttgtaatt
     3541 ccaaaacgcg gtctggccga agtccggcac accgcggttg tcaagcacgt cccggaagcg
     3601 tagccagggg ccagcagccg aggtgccggc gaccccccag agcaccagat cgaaatcacc
     3661 attctgcact gcggtggtta ccactggcgc ttcagggaac tgggtcgtga tgtcgaaccc
     3721 gacctcctgc gcaccttgcg cgaccaactc cagcgcggtc atccagtccg tccagccgta
     3781 cgggcactgc accgtccatg gccccaggcg ggtgccatcc tcaagtacgt agacgccgtc
     3841 ctccagtgtt gcgccgagct cctcctcaag gatctgccgt gcaacatccg ggttgtactc
     3901 ccagccgcca gtttcggcga gctcgtccgc tctaaagaac tcctcctccg caccttgggg
     3961 aatcaggagg ctgggattca ctggaacgga ataccgcgac atcgcggttt cggcgatctg
     4021 cgggtagttg atcgaatacg ccagcgcccg tcgcacggcc ggattgtcca ggccgggtct
     4081 ggaaacgttg atgtgcatca tcgggatcga ccccggcacg tgatatggtg cgtcgtcgta
     4141 ccaggtcccg accggcaagt cccgctccag ccacatctgc cagatctgcg gcgcaaactg
     4201 ctgggagaga tcgacctcgc cgcgctgcag ggcaagattg ccagcatcgt tggagtcgaa
     4261 gatcgggtgg acaatgttct tcggtacggg catgccgacg acatcggcgc cccagtagtc
     4321 ctcataccgg gtgatcacca cctgctcggc cgaggagatg gagagggtat acggcccaga
     4381 gccgaccggt tccatgtcca cgacactggc aacgccctcc gcctcgcgct cctcccagat
     4441 gtgctgtggc aatacatgaa tctgggtcag cagatgccgc accatgccgg gatgccggcg
     4501 ttccggattg taggtgagcc gtaccgtgcg ctcgtcaacc gcctccacct cggagacgta
     4561 gtcggtgacc gtgctgtacg gaatcccttc ctgccgctgc ggtagggtga tgctgtacac
     4621 cacgtcctcg gccgtgagcg gttcgccatc ctggaacgcc gtgccctcgt gcagcgtgat
     4681 ggtcactccc tcgtcagtct cttcaggaag atcagccgcg agcaacggct cgatctcgcc
     4741 tgtgacaacg ttgaaggtgt agagcgtctc gtagatatgg acgaagtagg tgtcaactgg
     4801 ccacggggca ttcgctgaaa gcgggttgaa ggtcgtcggg ggtccccact gcatgccggc
     4861 tacgtagagc gtttcgctgc gagccagctg tgcggcggat gcgccaggcg cgtatctggt
     4921 gaacgaagcg gccccaacgg ctgccattga gccaagcgcc gcaccatgtt tgatgatatc
     4981 gcgacgagag gataagtagc ctttcgacat cctgaaacct cctgatgtat caccaaagcc
     5041 gatatatcta gatgattggg cgcatttgaa agcaaatggc ggcccaacag cagaaatatc
     5101 tcggccttca gtctgatcta ttgtgtacgg gcatcataac atcaggcatt gtatgtgtcc
     5161 actggagatg tgagagttct gttgctgttg gaagttgcct cggacagagc ccagagtaac
     5221 gtactgggag cacacgccgc acgaaggtca acagcgcgcc ggtgcgaggc aaacaccggt
     5281 gtagctcgcc gcttttggac ccacccaggt atacgtttgg aacccctatg acgcccggac
     5341 gctccatagt catggtttaa ccttgcttgc agaaggcgtt tggaggtctc acctatctat
     5401 gtctttgcct caggctccat ccggcgacgc aaaacagggc acaccagatg ctccgcagat
     5461 cgaggggacg ctgcccgccg cgctcacccc gttgatcggt cgccaggaag agatcacccg
     5521 cataagccgc gtcctgcgcg aacgagcagc caggctcctt gtgctcacgg gtcccggggg
     5581 cgtgggcaag acacgcctgg ccctggccat cgccgaagcc accagcgatg cctatcagga
     5641 tggggtggct ctcgtcaatc tgggtccggt catcacctcc gatggcgtgc tgcctgccat
     5701 ctccgccgtc ctcggtgtac gtgaggccag ccaggatcac ctctatgccg ctgtacggga
     5761 atccctgaac aaccgcgaga tgctcctggt gcttgataac ttcgagcagg tcgtggaagc
     5821 agcaccgatc ctgactgacc tgcttaccgt gagtcctgcg ctcacgatac tggtcacgag
     5881 ccgttcggtg cttggcgtgt atggggaatt tgtcttcccc gttcctccca tgaaggtgcc
     5941 ggccacgacg gagagctcga tggagacgat cgagtcatcc gaagccgtgc agctcttcgt
     6001 caatcgcgtg cg
//
LOCUS       DASYWM010000124         1663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_154605, whole genome shotgun sequence.
ACCESSION   DASYWM010000124 DASYWM010000000
VERSION     DASYWM010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154605"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VGR29_05145"
     CDS             <1..569
                     /locus_tag="VGR29_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV2073010.1"
                     /translation="ATVVAGPIYSPTGQTGTIPPDERARKLETLVRNLSPILEVADAH
                     GIRLAVEPLNRFETSLFNTVRQAMELLELVDHPSLGLLLDTFHMNIEERDIGDAIRFA
                     GDRLFHFHACGNDRGAPGNDNIDWIAVRDALRAVNYTGTVTIESFTSANQTIATAASI
                     WRPLAETQDALATDGLAFLRNLFGSSQK"
     gene            complement(736..1098)
                     /locus_tag="VGR29_05150"
     CDS             complement(736..1098)
                     /locus_tag="VGR29_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073011.1"
                     /translation="MTSRDVTSLPRNPRIRTIPYNEQAITPIGDLLEVEVGTADFRLP
                     SSTVYQMTIPGVNGRPTAMVTLWPGIRRVDVVSGPATVVFTDVQTVDLVPGVEVQFRR
                     TAKECLILARNGQVIVRA"
     gene            1310..>1663
                     /locus_tag="VGR29_05155"
     CDS             1310..>1663
                     /locus_tag="VGR29_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV2073012.1"
                     /translation="MFAEGMPEEFGAMDSDIRSAVDIIGASSRTHDKPASPLNDDSKP
                     LWQQVTLDDVRAARERVMPHLHRTPMLRNRSLSRMTGTTIGLKAEVFQRTGSFKARGA
                     LNAAMQLTDEQKARGV"
BASE COUNT          319 a          524 c          502 g          318 t
ORIGIN      
        1 gtgcgacggt cgttgcgggg ccgatctact cccctaccgg gcagaccggc acgatcccgc
       61 ccgacgaacg agcacgaaag ctggaaactc tggttcggaa tctctctcca atcctggagg
      121 tcgccgacgc gcacggaatc cgccttgccg tcgagccgct caatcgcttt gagacaagcc
      181 tcttcaacac cgtcaggcag gcaatggagt tgctggagtt ggtggaccat ccgtccctgg
      241 ggctcctgct cgataccttc cacatgaata tcgaggagcg agacatcggc gatgcgattc
      301 gttttgccgg ggatcggctc ttccacttcc atgcctgcgg caacgatcgg ggtgcaccgg
      361 ggaacgacaa tatcgactgg atcgccgttc gcgacgcctt gcgggcagtg aactacaccg
      421 gaaccgtcac tatcgagtca ttcacgagcg cgaaccaaac gatcgcgacc gccgcctcga
      481 tctggcggcc gctcgccgag acccaggatg cccttgccac cgatggcctc gccttcctgc
      541 ggaatctgtt cgggtcaagc cagaaataag tcgtaggggc gaacccatag gcgattgcac
      601 agtaatcgag cgaatgcgtc cccttcatgt ttgcccttga ttgcgcctgc agggtgaaca
      661 tgaggggggc cactctgctc ggtgcatggc accgcgtact tgtttgcccc tacgcctcca
      721 tgatgcagtt gcccctcatg cccgaacaat gacctggcca ttcctcgcaa ggatgaggca
      781 ctccttcgcg gttcgccgga actggacctc cacgccaggg acgagatcca ctgtctggac
      841 atcggtgaag acgaccgtgg cggggccgga gacgacatcc accctgcgga tgccgggcca
      901 gagcgtgacc atcgctgtcg gccggccgtt cacgccagga atcgtcatct ggtagaccgt
      961 cgaactgggc agccggaaat ccgccgtgcc cacttccacc tccagcaggt cgccgatcgg
     1021 agtgatcgcc tgctcgttgt acgggatggt cctgatgcgc ggattgcgcg gcagcgatgt
     1081 cacgtccctg gatgtcatac gggcatcttt ccctggagag caattcgtcg tcccgaaagc
     1141 ttcggtatac tgacgcatcc gggtctaccc gggtggatgg atgatggcga ggaacagggc
     1201 ttatgtcgga atggaatgcg ccctgttgtc atcagttcta cacgtcgtgt cgtgtgccac
     1261 agcctgggct cggggcacct accagtatcg ttgacaggga gagtgcagga tgttcgctga
     1321 aggtatgccg gaagaattcg gggcaatgga ctccgatatc cggagcgcag tggacattat
     1381 cggcgcgagc tcccgcacgc acgacaaacc cgcctcccct ctgaatgatg acagcaagcc
     1441 actgtggcag caggtcaccc tcgacgatgt tcgcgccgcc cgcgagcgtg tcatgcccca
     1501 tcttcaccgg acgcccatgc ttcgtaaccg gtcgctgagc cggatgaccg gcacgacgat
     1561 cgggctcaag gcagaggttt tccagcggac cggttccttc aaggcccgtg gcgcgctcaa
     1621 tgccgccatg cagctcaccg atgagcagaa ggcacgtggc gtc
//
LOCUS       DASYWM010000125         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_23094, whole genome shotgun sequence.
ACCESSION   DASYWM010000125 DASYWM010000000
VERSION     DASYWM010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23094"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1049
                     /locus_tag="VGR29_05160"
     CDS             264..1049
                     /locus_tag="VGR29_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2073013.1"
                     /translation="MVQRSHLTADPDGSSLHDLISVRIVSSLLAGRPRHLAPARARSL
                     LPVIGADDVILPAFDDMLGDLAIRAQEGDQEARDALYAAFLPKLTRLMSPVRTPFAPP
                     GSQGIWNRDDVAQEAYLVFVELIEAWSGDVSFTAYVLSRFPWRLKDVIRRGIGKSPVP
                     PRQYGVPMEHAEPVADQEGNAEGVNAMLDALQEVLPEPLGVVLIAHVVHGMTKTEIAA
                     ELGVSRRTMVRYWQEIRVYTNCLLRDDARTGTDEPRSRRRRGR"
     gene            1367..2056
                     /locus_tag="VGR29_05165"
     CDS             1367..2056
                     /locus_tag="VGR29_05165"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEV2073014.1"
                     /translation="MSPIRRDDDFQRTEIEDWDTWIQEQIQEAQERGEFDNLPHAGKP
                     IQIWRTEVNPEYDLAFSRMKNADVLPVWMELDRDISRMSRDLDDFLERSTAWLLAERD
                     EAIQARVQEDPAEPDPSVPKRHWWQIWQRLMDWFRIEPDEADSRQEPRSIGDLLRLRE
                     HIRTQYLARAAALDKKIESYHNSLPEGLSHLQRLRMLPNRAAKRFDARFPVRLLLGET
                     SDDDLPEARSA"
     gene            complement(2287..>2886)
                     /locus_tag="VGR29_05170"
     CDS             complement(2287..>2886)
                     /locus_tag="VGR29_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEV2073015.1"
                     /translation="ASPAASPVAGSAGDVVAESTTEVDASFEELLASEEYAINVHESA
                     ENIGNYIACGNPTGPATDGELHIDLEELNDSGYTGQAHLVDNGDGTTTVTVTLMTMGT
                     GAGTPAASGQSDTAAAVEVEIKDFAYNPDPVTIKVGESVTWTNQDSAPHTATGQDREV
                     LQSGNLDQGQSYTQAFDTAGTYEYFCYFHPDMKGVVTVE"
BASE COUNT          521 a          875 c          933 g          557 t
ORIGIN      
        1 gcggagcatc tgctcgatct gcgggcactg aagaagcaaa cacaggagga tccctccgac
       61 aaggagatca tggtcagcta ggggtggttt gcagcgcgtg acagcgctcg acctctctcc
      121 gggtgaaggg cgccggggaa ctggacaccg cgggatgagc caggcgcgac aatcgataga
      181 gctccgaaga gcctccatgt gcatctatgc ggggcgccgc tcccggtcgg cagcggcgca
      241 tccatcggag gagttcttca ttcatggtcc agcgctccca tctcacggct gatccggacg
      301 gttcgtcgtt acacgacctg atatcggtcc gaattgtctc ttcattgctt gccgggcggc
      361 cccgccatct cgctcctgcg agggctcgct cgctgctgcc ggtcatcggg gcggacgatg
      421 tcatcctgcc cgcgttcgac gatatgctcg gcgatctcgc cattcgcgcg caggagggtg
      481 accaggaggc gcgcgacgcg ctctatgccg ccttcctgcc gaagctgacg cggctgatgt
      541 cccccgtgcg gactcccttc gcgccgcccg gctcccaggg gatctggaac cgtgacgatg
      601 tcgcgcagga ggcctacctg gtcttcgttg agctgatcga ggcgtggtcg ggcgatgtct
      661 cgttcacggc ctatgtgctc tcgcgctttc cgtggcgcct gaaggatgtg atccgcaggg
      721 ggatcggcaa gtcgcctgtt ccgccacggc agtatggcgt gccgatggag catgctgagc
      781 cggtagctga tcaggagggc aatgccgagg gcgtgaacgc gatgctcgac gccctccagg
      841 aggtcttgcc ggagccgctc ggtgtcgttc tcatcgccca cgtcgtccat gggatgacaa
      901 agacagagat tgcggcggag ctgggcgtga gccggaggac aatggtgcgg tactggcagg
      961 agatccgggt ctataccaac tgcttgctcc gcgatgatgc gcggacgggt accgatgagc
     1021 cgcgatcccg tcgtcgacgg ggcaggtaac gtcaggcgga gttatcctcg ccgtcgtgcg
     1081 tgcaggtcgc cactcttcag ggttgccggg aacgttggct gagcggggac gtcaatcagc
     1141 catcagatga ggatgatgca ggcatcgacc gtggcgatgg agccggagac gaccgcctcc
     1201 accgtatgtt tcaccattgg caccgggcat gcaaacgacc ccattgacac tacgaggcag
     1261 cccttcgatg gccgccgcag gggcacattg gcaactcttt tgccttttgt taccattttc
     1321 acaataagat ggatttccat gacaggagcg cacgaggagg tgcgacatgt cgccgatccg
     1381 acgcgatgac gatttccagc gcacagagat cgaggactgg gatacttgga ttcaggagca
     1441 gatccaggaa gcgcaggagc gaggcgagtt cgacaacctg ccgcacgccg gaaagccgat
     1501 ccagatctgg cggacggagg tgaacccgga gtacgacctc gccttcagcc ggatgaagaa
     1561 tgcggatgtg ctcccggtct ggatggagct ggatcgggat atctcccgga tgtctcgcga
     1621 tctcgatgat ttcctggagc gatcgacggc atggctgctt gccgagcggg atgaggccat
     1681 ccaggcgcgg gtgcaggagg atccggctga accagacccg tccgtcccga agcgccattg
     1741 gtggcagatt tggcaacggc tgatggactg gttcaggatc gaaccggatg aggccgattc
     1801 tcgacaggag ccccgctcga tcggcgatct cctgcgcctg agggagcata tccgcaccca
     1861 atacctggca cgcgcggcag cactcgacaa gaagatcgag agctaccaca actccctgcc
     1921 cgaggggctt tcgcatctgc agcgtcttcg catgctgccc aatcgggccg cgaagcggtt
     1981 cgatgcgcgc tttccggtcc ggctcctgct tggcgagacg tcggatgacg atctcccgga
     2041 ggcacgatcc gcctgatctc gagccacatg ccggtcggat acgatgcccc ctttgggaac
     2101 atccgaacca tgccagggat caaagggaga ggcggcgttg caaacagtgc aatgccgcct
     2161 ctgtgattcg tgccatcctg tctggcgaat cctgttaccc ctggctcagg gccatcggcc
     2221 cttcgctcaa cctgggttca ccgctggtgc caagtcagca gtcagccagc aaacgaatcg
     2281 gtgcccttac tcaacggtga cgacaccctt catgtccgga tgaaaatagc agaagtactc
     2341 gtaggtgcca gctgtgtcga acgcctgggt ataggactgc ccctgatcca ggttccctga
     2401 ctggaggacc tcccggtcct ggcccgtcgc ggtatgcggg gccgagtcct ggttggtcca
     2461 ggtcacactc tcccccacct tgatcgtgac cgggtcgggg ttgtaggcga agtccttgat
     2521 ctcgacctcg accgctgctg cggtatcgct ctgcccagaa gccgcaggtg tgcctgcacc
     2581 agtgcccatt gtcatcaggg tgaccgtcac ggtggtcgtg ccatcgccgt tgtcgaccaa
     2641 atgggcctgg cccgtgtagc cggagtcgtt cagctcctcc aggtcaatgt ggagctcacc
     2701 atcggtcgcc ggacctgtgg ggttgccaca ggcgatgtag tttccgatgt tttccgcgct
     2761 ctcgtgcaca ttgatggcgt actcctcgga ggcaaggagc tcctcaaaag aggcgtccac
     2821 ctcggtggtg ctctcggcga ccacgtcacc ggcactgcct gcaacggggg aagcggcagg
     2881 tgaggc
//
LOCUS       DASYWM010000126        14555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_586695, whole genome shotgun sequence.
ACCESSION   DASYWM010000126 DASYWM010000000
VERSION     DASYWM010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14555
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586695"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VGR29_05175"
     CDS             complement(<1..884)
                     /locus_tag="VGR29_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEV2073016.1"
                     /translation="MDLLSLLIIVAVLVLVLLLIIALAARLYRKVGPNMALIVYGFGG
                     TKVIKGSGKVIWPMIQQSRELSLELMSFDVAPTQDLYTNQGVAVNVEAVAQIKVKSDP
                     VSILTASEQFLSMNSQERNGLIRLVMEGHLRGITGQLTVEAIVKEPEMVADRMRANVA
                     EDMAKMGLEVVSFTIREVKDSNEYILNMGKPDIANVRRVADIATAEADRDTAIRRAQA
                     SREARIAEAAAEQEKVTAESMSQTKQAEAVRDLELKRAEFDRSVQEQRAQADKAYEIQ
                     ASVQQQRIMAEQVRIEQVR"
     gene            complement(1110..1697)
                     /locus_tag="VGR29_05180"
     CDS             complement(1110..1697)
                     /locus_tag="VGR29_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073017.1"
                     /translation="MFPINDALDAILLGGFLFGLLFSVGALLLGFADLGAHADAGDAG
                     GHGGHGGHGMLEGLFNVSSLLAFITWFGGIGYVVRNGLEFGTWLSLILGVLGGIGGAA
                     IVTWVILKLVQEDSEAMNPAAWSQVGVIGRVTSSIRSQGYGEIVYEQHGIRQVASAKA
                     SGASAIPRNTEVVVLRVERGVAIVEPFEDLLREHD"
     gene            1867..2649
                     /locus_tag="VGR29_05185"
     CDS             1867..2649
                     /locus_tag="VGR29_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2073018.1"
                     /translation="MSPDHADIPAADVSSAEPKYDATPIDPDSVTDEVRTRASLSFEA
                     VKGDPEVRELISSANRNLGVLGFTEHGFRHVGLVANIAHNVMARLHYDERHQELSQIA
                     GYMHDIGNVISRHGHASTSALLAHTILTRMGMNPEDVIVVMSAIGSHGDDSGRLGEPV
                     HPVSAALILADKSDVHRSRVRITDPADFDEHDQMNYATTSSFLRVDEETATVTLELQI
                     DTSMASVMKYFELFLPRMQMCRRAANLLDQTFRITINDVAVL"
     gene            2817..3974
                     /locus_tag="VGR29_05190"
     CDS             2817..3974
                     /locus_tag="VGR29_05190"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV2073019.1"
                     /translation="MISSRQRKVTVPGYSVSIDRWKDFDPMLFVTVVVLMGFGIVSIW
                     SAVGLPPLLSNNDGTKQAIYGLIGLGLMFFFASFDYRYLESMTWPLYIAGILLLVAVQ
                     SPLGVTIAGAQNWIDVGFTLIQPSEFTKITTILALASFVASQGDEMKRFSNFVIAGMI
                     VALPSGLILIGPDLGQTMVYVALWTSSLLVMRTSRLWLFGLFVTMPLLVWFAWNFVMA
                     DYQKLRFIVSYQPELDPLDKGYQIIQARIAIGAGGVFGEGLRGGTQSTLNLVGVTESD
                     FIFAHASGMFGFIGMLGLLIAQVILLWRCLTVAEVARDHFGQMIAVATTGVVFFQAVL
                     NIGMNVGLMPVAGITLPFVSAGVSSLWSFMILIGILQSIRMHHRKLAFQPL"
     gene            complement(3971..4798)
                     /gene="rsmI"
                     /locus_tag="VGR29_05195"
     CDS             complement(3971..4798)
                     /gene="rsmI"
                     /locus_tag="VGR29_05195"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV2073020.1"
                     /translation="MGTLYVVATPIGNLQDLTPRAVETLRSVSLIAAEDTRHSGTLLK
                     AYGIGTRMVSYHHHNRSQRESQLLRELEAGDVALISDAGTPGIADPGREIVEAARQAG
                     HRTVPIPGASSVVAAVSASGLVEGPFIFLGFLTRKGEERRAELGKAAASGWPLVLFES
                     PVRLAATLADLHAAVGDREATVARELTKLHEELVTDSLQALRDRYAGQEVRGEIVIVV
                     GPAAAGGIGGTDVRALARRLLADGIKPSKAAREVAAIAGIPGDEAYALVREVSTERD"
     gene            4944..6953
                     /gene="dnaX"
                     /locus_tag="VGR29_05200"
     CDS             4944..6953
                     /gene="dnaX"
                     /locus_tag="VGR29_05200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV2073021.1"
                     /translation="MTDGSLFNLDALETRPVAEPSAPEPYTPTPSSAGTTPAARDAAA
                     PRSLYRKYRPQSFDSDDLFGQEHIVNTLRNAIALDRIAHAYLFCGPRGTGKTTTARLL
                     AKAVNCLDPDPGNRPCNQCEACIAINTGASTDVIEIDAASNRGIDDIRDLRERVKYAP
                     SQLQTKFYIIDEAHQITGAAANAFLKTLEEPPAHTKFILATTDPEELLQTIVSRCQRF
                     DFRRISLDAMTACLAKVAAAENIDIDQDAKTAIARHATGSMRDALGLLDQIAVYRERV
                     DDEGTRVTVDLMRTVLGVSKNERVEEIATALADRDAGAGLRVVGQAVEAGDDIRQLAR
                     QLLAYLRLLLLERAGGTSDLDETGKVLAKRFELGELAELTRLMGEIDFKVKHATISQL
                     PLEIAIVEGALRGQHTQQSADSQDRRTAPSAPQAQEERSQYTPPTAAPQPEPIVPKTS
                     WRDKVRGEDRGSRSASPERPREEVKPPGNETPPPPWEEQPTQPAPERVSASAPVTVPT
                     PAVLANGALQLEAVVDGWGRIKADVKAVNRRIEALLQQIDPIAINGNKITLVTPYEFH
                     RNRVNTDEVRAVIEEVIARQFGAKVTVGCITRSEWSSTPQIQQAPSAQEPDIPQPRQS
                     AASPGPQVEGPPAVEEATDPPQVIDTERQIEAMKNIFDAEIEETE"
     gene            complement(7021..8340)
                     /locus_tag="VGR29_05205"
     CDS             complement(7021..8340)
                     /locus_tag="VGR29_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073022.1"
                     /translation="MTLVCSVVFAACGPAAETDDVPEDRKFANDPVTERPLTPMVGPT
                     ELATPASARPIVEVSPETLLRSRGAPDTLYTLSNDTLTAVTVDSGEGQREGIALPKNR
                     RILDFDGSPTGDSVAVLVASETDDALFVAFYRSTGEQVGEEQQVFMQEGMTATPDTAE
                     SQQSYTVSWSPQGTGVLLSSSTALERVDVEGGAEPVPLGDIEGTLQRAAWSPQGTRIL
                     LQLLQEDGSQRVYVRDLEEDKTREVEALQTSGSTELQELQWLPDGSGLVFVRGQMVNG
                     VALRGQVFVYPLSEEVPRLVATSGQGGPTATITDLAVAPDGDSVAYVISVLDGTEWAF
                     HSMWVRSMDGNMSYQVPVDPRGVVTQLAWIDRGIAWEQRASSMNEVGDIMFLRWDQDA
                     ALLLAPAPSEATPVGTPGASPVVPLQGTPGASPVATPIGTPGATPAE"
     gene            8441..9358
                     /locus_tag="VGR29_05210"
     CDS             8441..9358
                     /locus_tag="VGR29_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV2073023.1"
                     /translation="MTEQDSLAEILVRSRVEPEYTMGALAHQTGVPADTLRSWERRYG
                     FPTPARTETNQRRYSERDIAAVRWLRAQKERGQGISEAIAMVTSRLVAGPTTASERFP
                     DSFNTSTQTPPLQGLVEALISGQLDAAQNAWDDIVVALSPGAIGSDVILPAHQRIEQT
                     VGATTGTSPSRDRAHAFLLRKATVLLDHAGPDAGQISFCILTSGTQEDAVPATVLATA
                     LARAGFRLYTPFLNAASLESVHALHDLRPDHVIFVGGSADDARTVARLLPGQCIYRWS
                     PDPSTTPDQYPTILPASLTAVEAFFQQDH"
     gene            9447..10385
                     /gene="murQ"
                     /locus_tag="VGR29_05215"
     CDS             9447..10385
                     /gene="murQ"
                     /locus_tag="VGR29_05215"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEV2073024.1"
                     /translation="MSSPHEGQETAEIDLSRVSTEQRNPLTADIDTVDTTQMLRIINA
                     EDARVPGAVAAQIPAIARAVDGIVERIRNGGRLVYIGAGTSGRLGVLDASECPPTFST
                     PPELVVGLIAGGDHALRHSVEHVEDQPESGADALRSIKVSDKDTVVGIAASGRTPFVL
                     GAIAYANEIGALTVGICNSDHAKLSDLVHIAIPVITGPEVITGSTRLKAGTAQKLVLN
                     MLTTGAMIQVGKTYGNLMVDLQPVNQKLRVRAIGIVRDAAGIPEDEAATALQHADGDV
                     KTAIVSTLLGVSPEQARNRLTAAQGRIRNALKSGPA"
     gene            10382..11302
                     /locus_tag="VGR29_05220"
     CDS             10382..11302
                     /locus_tag="VGR29_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEV2073025.1"
                     /translation="MTVPLFAGIDGGGTKTAIVVVDAEGIPVFRGQTSTSNAAVIGHE
                     RAAETLHRLMLSAASACNTDLPFTAAWFGLSGSDRPEDHRILRPRLADTATEIMMTND
                     AELALGGLLGRVGVAIVAGTGSICFGQNSEGMHARAGGWGHIFSDEGSGYGIAVQALR
                     AFAAQHDGLGPETSLTSRLTEHWEIEDPFSIITHVYHPETTKGDIALLSKILVEEAAK
                     GDTVSLQIMEQGAECLAAYGTSVARRLDLGPELALVFVGGMLVPVKPYRERVLHLLRE
                     HWVIPEVTLVEDPALSAAQSLAQTYRSAAS"
     gene            11299..12459
                     /gene="nagA"
                     /locus_tag="VGR29_05225"
     CDS             11299..12459
                     /gene="nagA"
                     /locus_tag="VGR29_05225"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV2073026.1"
                     /translation="MTQLSAFAVRGKLLLGTHLEEGTLVVEDGLLTNVHRGHIRDGNL
                     PETVIDAAIISPGLIDLQVNGAYGREVTANPDDIDDISRNLLSTGCTAWLPTVVTAPA
                     EFYPPIFEGWLNHRVEAGAVPLGFHLEGPFISTEKKGAHRIECIEAASEKLFESWLEQ
                     ESILLVTLAPERKGATGRIRRLTQRGVLVSLGHTNATYEEFVCGIDAGARKATHLFNT
                     MTSIHHRAPGAMVATLVDDRITAGLIPDGVHSHPATIRLALKSKGADGIVIVSDMMSA
                     CGLGPGIYSLSTKQVTVDATSARLADGTLAGSILTMDQALRNLVGWSDATPAEALHMM
                     TAVPARLLGDEQRGRIVAGTRADLTLWDPALQPIRTIVGGRVGWERERATTG"
     gene            12533..12844
                     /gene="gatC"
                     /locus_tag="VGR29_05230"
     CDS             12533..12844
                     /gene="gatC"
                     /locus_tag="VGR29_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV2073027.1"
                     /translation="MSLTRKDVEHVASLARLGLSDDEIDMLQDQLSSILGHIEVLNQL
                     DTEAIPPTAQVIQLENVLRPDEMRPSLPQETVVTMAPKTRDGFIAVAAVMGGNHDGGV
                     A"
     gene            12841..14313
                     /gene="gatA"
                     /locus_tag="VGR29_05235"
     CDS             12841..14313
                     /gene="gatA"
                     /locus_tag="VGR29_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV2073028.1"
                     /translation="MSLPADLTLMTAAQARSLLDNREISSVELTRAYFERIEEVEPEI
                     HSYLHVMTDVALTQASDADRRIEEGDIDLLTGVPIALKDILCTTDAPTTAASKILEGY
                     VSPYDATVVTRLRDQGAVFLGKTNTDEFAMGSSTENSAYGETHNPWDTSRVPGGSSGG
                     SAAAIAAREATVSLGSDTGGSVRQPAGFCGIVGLKPTYGRVSRYGLLPFASSLDQIGP
                     FARTVEDAAIMLRAIAGKDPLDSTSVPVEVPDYLADLRQDLKGVRIGVAPQYTVSGME
                     PGVEAAVKVAIKQLEDLGAEIVEVSLPHTEYALATYYITAPAEASANLARYDGVRYGN
                     RVEGDNLIDTYLKTRGRGFGPEVKRRIMLGTHALSSGYYDAYYVKAQKVRTLIKQDFD
                     VAFESCDVVVAPTSPTIAFKSGSRTSDPYQMYLADIFTIPANMVGIPGISVPCGFSDG
                     MPVGLQFLGKAFDESRLLGVAHAYEQAAGWYLKLPEIPAG"
     gene            14396..14470
                     /locus_tag="VGR29_05240"
     tRNA            14396..14470
                     /locus_tag="VGR29_05240"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14428..14430,aa:Lys,seq:ctt)
BASE COUNT         2880 a         4746 c         4125 g         2804 t
ORIGIN      
        1 cggacctgct cgatgcggac ctgctcagcc atgatcctct gctgctgcac actcgcctgg
       61 atctcgtaag ccttgtcggc ctgcgcccgc tgctcctgta ccgagcggtc gaactccgcc
      121 cgcttcagct cgagatcccg gacggcctcg gcctgtttgg tctggctcat tgattcagcc
      181 gtcaccttct cctgctcggc ggcggcttcg gcaattcggg cctcacgact cgcctgtgcc
      241 cgccgaatcg ctgtatcgcg gtcggcttct gccgtcgcga tatccgccac gcgcctcaca
      301 tttgcgatgt cgggtttgcc catgttgaga atatattcat tggaatcctt gacctcgcgg
      361 atggtgaacg acaccacctc gagccccatc tttgccatat cctctgcgac gttagctcgc
      421 atccggtcgg caaccatctc cggctctttc acgatcgcct caaccgtgag ctggccagtg
      481 atgccacgca ggtgaccttc catgaccaga cggatcaatc cattgcgctc ctggctgttc
      541 atgctgagga actgctcgga ggcggtcagg atgcttaccg ggtccgactt cactttgatc
      601 tgggcaacag cctcgacatt aaccgccaca ccctggttgg tatagagatc ctgggtcggt
      661 gcgacatcga agctcatgag ctccagcgag agctcacggc tttgctggat catgggccag
      721 ataaccttgc cgctgccctt gatgaccttg gttccaccga agccatagac aatcagggcc
      781 atgttcggcc cgaccttccg atagagacgc gccgccaggg cgataatcag caacagcacc
      841 aaaaccagta cagcgacaat aatgagcagt gaaagcagat ccattatgtt tccttccgat
      901 cacgaccagg cctctgccag ccgtgatcct tcatacttgc cgcggcgcct cgcgagtacc
      961 tcgcttggga ggagtctcac cgcagccttg atgtatcccc atagtggcaa cgacgaaagc
     1021 cccggtacgg ttcgatgtgg cggttatcga ccttccctgc aggtgacccg aagcaacccg
     1081 atctcgatac agcgtcctca agctgaagcc tagtcgtgct cgcgtagcag atcctcaaat
     1141 ggctccacaa tcgcgacacc ccgctccacc cgcagcacga ccacctcggt attccgcgga
     1201 attgccgatg cacccgatgc tttcgctgag gcaacctgcc tgatcccatg ctgctcgtac
     1261 acgatctcgc catacccctg cgaacgaatc gatgatgtga ccctgccgat aacaccaacc
     1321 tggctccacg ccgccgggtt catggcttcg ctgtcctcct gcaccagctt caggatgacc
     1381 caggtcacaa ttgcagctcc cccgatccct ccgagtaccc cgaggatcag ggagagccac
     1441 gtcccaaact ccagcccgtt gcgcaccaca tacccgatcc caccaaacca ggtaataaac
     1501 gccaggaggg aactgacgtt aaacaggccc tccagcatgc cgtgtccgcc gtgcccacca
     1561 tgccctccgg catcgccagc gtcagcatgc gctcccaagt ccgcgaaccc cagcaggagt
     1621 gcgcccacgg agaagagcaa cccaaagaga aatccgccga gaagaatggc atccaatgcg
     1681 tcgttgattg gaaacatcgc gagctcccgg tgcatctgaa tgagcatgta cagatagtgt
     1741 acgcttttgg attgaccgcg ttacacggtc acgctctgga tgcgaccgac ctcttcaaac
     1801 ttcgcgtcac cactcacagc taatgatggc ggctggacag tttctcgcca tccctgcagg
     1861 agtcccatga gtcccgacca tgccgatatc ccggccgccg acgtatcgtc agccgagcca
     1921 aaatacgacg ccacgccgat cgacccggat agcgtcaccg acgaggtacg cacccgggcc
     1981 agtctctcct tcgaggcagt aaagggcgat ccggaggtca gggagctcat ctcatccgcc
     2041 aaccgcaacc tgggcgtgtt ggggtttacc gagcacggat tccgccatgt cggtctggtc
     2101 gcgaatattg cccacaacgt catggcgcgg ctgcattacg acgagcgcca tcaggagctt
     2161 tcccaaatcg ccgggtacat gcacgacatc ggcaacgtca tctcccggca cggacatgca
     2221 tctaccagcg cgctcctggc acacaccatc ctgacgcgga tgggcatgaa cccggaggac
     2281 gtcatcgttg tgatgagcgc gatcggctcg catggggacg actccgggag gttgggtgaa
     2341 ccggtgcatc cggtgtctgc agcactgatt ctcgcggaca agtcggatgt tcaccggtcg
     2401 cgtgtccgga tcacggaccc ggcagacttc gatgagcacg accagatgaa ctacgccacc
     2461 acatcgtcgt tcctccgggt ggacgaggaa acggctacag taacgctgga actccagatc
     2521 gacacgtcga tggcctccgt gatgaagtac ttcgagctct tcctgccgcg tatgcagatg
     2581 tgtcgtcgcg ccgcgaacct gctcgaccag acgttccgca ttacaatcaa tgacgttgcg
     2641 gtgctttagt aggcgttatt ctgcccgttc ataccttggg gatgtggtgg acacaaccag
     2701 gtgccaacga gcagatgcgg tgctcatact gacggcaccc gagtatattc ccgccctacc
     2761 cgaaccaatc tcctgacatc aagcttcatc gtctgggcaa ccagggacac acgatcatga
     2821 tttcttcacg ccaacgcaag gtgaccgtcc caggctacag cgtcagtatc gatcgctgga
     2881 aggatttcga tccgatgctt ttcgtcacgg tcgtcgtcct catggggttc ggcatcgtat
     2941 ccatctggag cgccgtcggt cttccgccgc tcctcagcaa taacgacggg acgaagcagg
     3001 ccatctatgg cctcatcggc cttggactga tgttcttctt cgcctcattc gattaccgct
     3061 atctggaatc catgacatgg ccgctctaca tagcaggcat tctgctgctg gtcgcggtgc
     3121 aaagtccgct tggcgtgacg atcgccggcg cacaaaactg gatcgatgtc gggtttactc
     3181 tcatccagcc gtccgagttc accaaaatca ccaccatcct ggcactggcg agcttcgttg
     3241 cctctcaggg cgacgaaatg aagcggttca gtaacttcgt cattgcgggc atgatcgtcg
     3301 cacttccctc agggctcatc ctgatcggtc cggaccttgg ccagacgatg gtctacgtcg
     3361 ccctttggac gtcctctctc ctcgtaatga gaacgagccg gctctggctt ttcggcctct
     3421 tcgtcacgat gccgcttctc gtctggtttg catggaactt cgtgatggcg gactaccaga
     3481 agctccgctt catcgtctcc tatcagccgg agctggatcc gctcgacaag gggtatcaaa
     3541 tcatccaggc gaggattgcc ataggtgccg gaggggtctt cggcgaaggg ctgcgtggtg
     3601 gcacacaaag cacactcaac ctcgtcggcg tcaccgagtc ggatttcatc ttcgcccatg
     3661 ccagcggcat gtttggcttc atcggcatgc ttggtttgct gattgcccag gtcatccttc
     3721 tctggcgctg cctcactgtg gcggaggttg cccgcgacca ctttggccag atgatcgcgg
     3781 tcgcgacaac cggcgtggtc ttcttccagg ccgtcctcaa catcggcatg aacgttggcc
     3841 tgatgccggt cgccggtatc acccttcctt tcgtcagcgc cggcgtctcc tccctctggt
     3901 cgttcatgat cctgatcggg atcctccaga gtatccggat gcaccaccgg aaactcgcgt
     3961 tccaaccgct ctagtccctt tccgtgctta cctcacggac gagggcgtag gcttcatcgc
     4021 ccgggatacc ggcgatggca gcgacttccc gcgcggcctt gctcggcttg atcccgtccg
     4081 cgagaaggcg gcgcgctagc gctcgcacgt cggtgccgcc gatccctccc gctgccgcag
     4141 ggccaaccac gatgacaatc tcgccccgaa cctcttgccc tgcataacga tcccgtagcg
     4201 cctgaagact gtccgtaacc agttcctcgt gcagcttcgt caactccctg gctactgtag
     4261 cttcgcggtc gccaaccgct gcatggagat cggccagcgt ggcggccagc cggactgggg
     4321 actcgaaaag gacaagcggc caacccgatg ccgctgcctt tccaagctcg gctcgtcgct
     4381 cctcgccctt ccgcgtcaga aagccgagaa aaatgaacgg cccctcaacc agtccggacg
     4441 cgctgacggc agcgacgaca gaggacgctc cgggaatcgg caccgttcga tgtcccgcct
     4501 ggcgcgccgc ctcgacgatc tcccgtccag gatctgcgat cccgggtgtt ccggcgtcgg
     4561 agatgagtgc cacatcaccc gcctccagtt cccgaagcaa ttgactttcc cgttggctgc
     4621 ggttgtggtg atggtatgag accattctgg tcccgatccc atacgccttc agcaacgtgc
     4681 cgctgtgccg cgtgtcctcg gccgcgatga gactcactga gcgcagcgtt tccaccgccc
     4741 tcggtgtgag gtcctgcagg ttcccgatcg gcgttgcgac cacatagagc gtgcccatac
     4801 agcagatcga ccttcattga ggggtgaacg tatagccggg tatacttgtc ccctatctca
     4861 ccacacgctg gctccatatg ccatgcccgg tgaatcccgc gctttcccgg aatgaagtac
     4921 atagcggaga cagatcagca ccgatgacgg acggctcgct ttttaatctt gatgcccttg
     4981 aaacacgccc cgtagcggag ccatccgctc cggagccata tacccctacg ccgtcttctg
     5041 cgggaacgac gcctgccgcc cgcgacgccg ctgccccgag atcgctatat cggaagtacc
     5101 gaccccagag ctttgattcc gatgatctct tcgggcagga acacatcgtc aacacattgc
     5161 gcaatgcgat cgcgctggat cgcattgcgc atgcctatct cttctgtggt cctcggggca
     5221 ctggcaagac aacgactgct cgactgctgg cgaaggccgt caattgcctt gatcctgatc
     5281 ctggcaaccg gccctgtaat cagtgcgagg cctgtatcgc gataaatacc ggcgcgagca
     5341 ccgatgtgat cgagattgat gccgccagca atcgcggtat cgatgacatc cgggatttgc
     5401 gtgagcgcgt caagtacgca ccaagccaac tccagaccaa gttctatatc attgacgagg
     5461 cccaccagat caccggcgct gccgccaatg cctttctgaa aacgctcgag gagccccccg
     5521 ctcataccaa attcattctc gccacgacgg accccgaaga gctccttcag acgattgttt
     5581 cccgctgcca gcgtttcgac tttcgtcgta tcagcttgga cgccatgacg gcctgtctgg
     5641 ccaaagtggc ggcagccgag aatatcgaca tcgaccagga cgccaagacc gcgatcgctc
     5701 gccacgcgac aggttccatg cgcgatgcgc tcggcctgct tgaccagatt gcggtctatc
     5761 gcgaacgagt cgatgacgaa ggcacccgcg tcacggttga cctcatgcgc acggttcttg
     5821 gtgtcagcaa gaatgaacgg gtcgaggaga tcgctacggc tctcgctgac agggatgccg
     5881 gtgccgggct tcgcgtcgtc ggacaggcag ttgaggcggg tgacgacatt cggcagctgg
     5941 cacgccaact ccttgcctat ttgcgccttc ttctcctgga acgcgctggt ggaacgtcag
     6001 acctggacga aaccggcaag gtgttggcca agcggttcga actgggcgag ctggcggagc
     6061 ttacgcggtt gatgggcgag atcgacttca aggtcaagca cgcaacaatc tcgcagctcc
     6121 cactggagat cgcaatcgtc gaaggcgccc tccgcggcca gcacacgcaa cagtcggccg
     6181 attctcagga tcgacgcacc gcgccttccg ctccccaggc acaggaagaa cgctcccaat
     6241 acacaccgcc gactgccgct ccccagccag agccgatcgt gcccaagacg tcctggcgag
     6301 acaaggtgcg gggagaggac cgcgggtcac ggtcggcgtc accggaacgt ccccgcgagg
     6361 aagtgaagcc gccgggaaac gagactccgc ctccaccttg ggaggaacag ccgacccagc
     6421 cggcaccgga gagggtttca gcctccgcac cggtcacagt gcccactccg gccgtgttag
     6481 ccaacggggc acttcagctg gaagcggtgg tcgatggctg gggccgtatc aaggcagacg
     6541 tgaaggcggt gaatcgccgg atcgaggcgc tgctccagca aattgaccct atcgcgatca
     6601 acggcaacaa gatcacgctg gtgaccccgt acgagttcca tcgcaaccgt gtgaatactg
     6661 acgaggtgcg cgcggtcatc gaggaggtta tcgcgcgcca gttcggtgcg aaggttactg
     6721 ttggctgcat cacaagaagt gaatggtcga gcacgcccca gattcagcaa gcgcccagcg
     6781 ctcaggaacc cgacatcccg caacccaggc aaagcgcggc gtctccaggg ccccaggtcg
     6841 aggggccccc tgccgtggaa gaagcgacgg acccacctca ggtgatagat accgagcggc
     6901 agattgaagc catgaagaac atctttgacg cggagatcga agagacggag tagcttgggc
     6961 ctctgcacca gcccatgaca actcagggcc tcccaggccc aactactccc ccaagggcgt
     7021 ctattccgca ggagttgcgc cgggggtccc aatcggcgtg gctacgggac ttgcgccggg
     7081 tgttccctgc aatgggacta ccggactggc acccggggtt cccaccggtg tcgcttctga
     7141 tggtgccggt gcgagcagca acgccgcgtc ctggtcccat ctcaggaaca tgatgtcgcc
     7201 gacctcgttc atcgatgacg cgcgctgctc ccaggcgata ccgcgatcga tccatgccag
     7261 ttgcgtcact acgcccctgg ggtcgacagg cacctggtag ctcatgttcc cgtccattga
     7321 gcgcacccac atactatgga aggcccactc ggtcccgtcc agcaccgaga tcacataggc
     7381 gaccgagtct ccgtccggcg caacagcaag gtccgtgatc gttgcggttg gaccgccttg
     7441 ccccgaggtg gcaactagcc gcggtacttc ctcggagagc ggataaacga aaacctggcc
     7501 gcgtagcgcc acaccgttga ccatctggcc ccgaacaaaa accagacccg agccgtccgg
     7561 cagccactgc agctcttgca actctgttga gccacttgtt tgcagtgctt ctacctcacg
     7621 cgttttatct tcctcgaggt cgcgcacata gactcgctgc gatccatcct cctgaagaag
     7681 ctgaagcagg atccgcgttc cctgagggga ccacgccgcc cgttgcagcg tgccttctat
     7741 atcgcccaac gggacaggct cagccccacc ctcgacatct actcgctcga gagcggtgga
     7801 gctgcttaga aggacacccg ttccttgcgg cgaccaggaa acggtatagg attgttgaga
     7861 ttccgcagtg tcgggcgttg cagtcatgcc ctcctgcatg aagacctgtt gctcctcgcc
     7921 tacctgctcg cccgtggatc gatagaatgc gacgaacagc gcatcgtccg tctcggatgc
     7981 caccagcacc gctacgctgt ctccagtggg ggaaccgtcg aagtccagga tgcgccgatt
     8041 cttgggtagc gcaatccctt cccgctgccc ctcaccgctg tccacagtga ccgccgtcaa
     8101 ggtatcgttc gacaaggtgt agagagtgtc cggagctccc cgcgaacgca ggagcgtctc
     8161 gggggacacc tcaacgatgg gcctcgccga tgcgggcgta gccagttcag tcggcccgac
     8221 catcggtgta aggggcctct ccgtcaccgg atcgttagcg aacttgcgat cttcgggcac
     8281 atcgtccgtt tccgccgccg ggccgcaagc agcaaacacc accgagcaaa ccagcgtcat
     8341 gaggaacacc gccagccagg gcctgggccg tccgggatca tgtttatttg gttcgtttgt
     8401 acacaccttc aaggatggga ttactcgctc atgtatggga atgactgaac aggattcact
     8461 ggcagaaata ctggtccgct ctagggttga gcctgaatat accatgggtg ccctggctca
     8521 ccagacaggt gtgcctgcag atactcttcg ctcatgggag cgcaggtacg gctttcccac
     8581 gcctgcacgg actgagacca atcagcgtcg atattcggag cgcgacatcg ccgccgtccg
     8641 atggttacga gcacaaaagg agcgtggcca gggtatcagt gaagccatcg ccatggtcac
     8701 gtctcgactg gttgcgggac cgaccacagc ttcggaaagg ttcccggatt cattcaatac
     8761 ctcaacgcaa acgccgccac tccagggatt ggtggaggcg ctcatctccg gtcaactcga
     8821 tgcagcgcaa aatgcctggg acgacatcgt ggtcgctctc tctcccgggg ctatcggcag
     8881 tgacgtgatc cttccggcgc accaacgtat cgaacagacg gtcggggcaa caacaggtac
     8941 atctccatca cgtgaccgcg cccacgcctt cctgctccgc aaggcaactg tgctcctaga
     9001 tcacgccggt ccagacgccg gccagatttc attctgcatc ctgacgtcag gaacccaaga
     9061 ggacgccgtg cccgctacag tacttgcgac cgcgcttgcg cgtgccggct tccggctcta
     9121 tacgccattt ctcaacgccg cctcgttgga gagcgtgcat gccctccatg acctgcgccc
     9181 agaccacgta atttttgtag gcggatcagc tgatgacgca cgcaccgtcg cccggctgct
     9241 ccctggacag tgcatctatc gttggtcccc cgacccctca accaccccgg atcaataccc
     9301 cactatcctg ccagcttcgc tcacagcggt cgaagcgttt tttcagcaag atcactgatc
     9361 ccgattgcat ccccccacct ctcggggcac actctcaaca ggcgtgacag aactggccac
     9421 gagaggaagt gaggcaccga gatcccatga gttcaccaca tgaaggacag gaaacagcgg
     9481 agatcgatct ctcccgcgtc tcgacggagc agcggaatcc cctaaccgcg gatatcgata
     9541 ctgtcgacac cacgcagatg ctccggataa tcaatgccga ggatgctcgc gttccgggtg
     9601 cggtcgccgc gcaaataccg gccattgcca gggcagtcga cggcatagtc gagcgcatcc
     9661 gaaacggtgg ccgtcttgtc tatataggcg caggaacctc agggagactc ggtgtgctgg
     9721 acgcatccga gtgccccccc accttcagta ccccaccgga actcgtcgtt ggcctgattg
     9781 ctggcggcga ccacgcgctt cgccactccg tcgagcacgt cgaggatcaa ccggaatcag
     9841 gcgccgatgc cctgcgctcg atcaaagtca gcgacaagga caccgtcgtt ggcatcgccg
     9901 cgtccggccg cacccctttt gtcctcggcg ccattgccta tgcgaatgaa atcggcgcgt
     9961 taaccgtcgg tatctgcaac tccgaccatg cgaagctgtc cgatctcgtc catattgcta
    10021 tcccggtcat caccggacca gaggtgatta ctggctcgac ccggctcaag gctggcacgg
    10081 cgcaaaagct tgtcctgaac atgctgacga ccggcgcaat gatccaggtt ggcaagacct
    10141 acgggaacct gatggtggat ctgcagccag tgaaccagaa gctgcgcgtc cgtgccattg
    10201 gtatcgtgcg tgatgccgca gggattcccg aggacgaggc cgctaccgcg cttcagcatg
    10261 cggacggaga cgtcaagacg gcaatcgtca gtacccttct cggcgtctcc ccggagcaag
    10321 cccgcaatcg cctgacggct gcgcagggac gaatccggaa cgcattgaaa agcggtcctg
    10381 catgaccgtt cccctcttcg ccgggatcga tggaggtggc accaagacag ccatcgtcgt
    10441 cgtcgatgcg gaaggcatcc ctgtctttcg cggtcaaacc agcacctcga atgcagccgt
    10501 catcggtcat gaacgcgccg cggagaccct gcaccggctc atgctgtctg ccgcatccgc
    10561 gtgcaacacc gacctgccct tcaccgccgc ctggttcggg ctctctggct ccgaccggcc
    10621 ggaggatcac cgcatcctcc ggccccggct cgcggacacc gcgacggaaa tcatgatgac
    10681 gaacgatgcc gaactagcac ttggtggcct gctcggcagg gtcggcgtcg cgatcgtcgc
    10741 cggcaccggc tcaatctgct ttggccagaa cagtgagggg atgcatgcgc gtgccggagg
    10801 atggggacac attttcagcg atgaaggcag cgggtacggc atcgcggtgc aggcgctgcg
    10861 tgcattcgcg gctcagcacg atggactcgg cccagagacg agcctgacgt cccggttgac
    10921 ggagcattgg gagattgaag accccttctc tatcatcacc cacgtctatc acccggagac
    10981 caccaagggg gatatcgctc tgctgtcgaa gattctcgtg gaggaggcag caaaaggaga
    11041 tacggtatcc cttcaaatca tggaacaggg cgcggagtgc ctggcggctt acggcacctc
    11101 ggtggcccgg cgccttgacc tcggccccga gctcgcactt gttttcgtcg gtggcatgct
    11161 ggttcccgtg aaaccctacc gggagcgcgt gctccacctt ctgcgtgagc actgggtgat
    11221 cccggaagtc acgctcgtcg aggatcccgc gctatccgca gcccagtctc tcgctcagac
    11281 ataccggagt gccgcttcat gacccagcta tccgcgtttg ccgtccgcgg caaactgctc
    11341 ctcgggacgc atctggaaga aggcacgctc gttgtcgagg acgggctgct gaccaacgtc
    11401 caccggggac acatccggga cggaaatctt cccgagacag tgatcgacgc cgccataatc
    11461 tcacccggcc tcatcgactt gcaggttaac ggcgcctatg gcagggaggt gacggcgaat
    11521 cccgacgaca tcgacgatat ctcgcgaaat ctgctctcaa cgggatgtac ggcgtggctg
    11581 ccaacggtcg tcaccgcacc ggccgagttt tacccaccta tcttcgaagg ctggctcaat
    11641 catcgtgtcg aggcgggcgc ggtgccgctc gggttccatc tcgagggccc attcatctcc
    11701 actgagaaaa agggcgcgca ccgcatcgaa tgcattgagg ctgcaagcga gaagctcttc
    11761 gaatcgtggc tcgagcagga atcgattctg ctcgtaacgc tggccccgga gcgtaaagga
    11821 gcgaccggac gcattcggcg gctcacccag cgcggggtcc tcgtgagcct cggccacacg
    11881 aatgccacgt atgaggagtt cgtctgtggc attgatgccg gagcccgcaa ggcaacccac
    11941 ctcttcaata cgatgacctc gatccatcat cgggcgccgg gggcgatggt ggccacgttg
    12001 gtggatgacc gcatcacagc ggggctgatt cccgacggcg ttcactcgca cccggcgacg
    12061 attcgcctgg cgctgaagag caagggcgct gatggcattg tgatcgtctc ggacatgatg
    12121 tccgcttgcg ggcttggtcc cggcatctac tcgctgtcca caaaacaggt caccgtcgac
    12181 gctacatcag cgcgcctcgc ggatggcacc ctcgccggga gcatcctcac catggaccag
    12241 gcactgcgca acctcgttgg ctggtcagat gcgacgcccg cagaggcgct gcacatgatg
    12301 acggccgtcc cggcacgcct gttgggagat gaacagcgcg gacgcatcgt cgccggcaca
    12361 cgcgccgacc tcacgctgtg ggacccagcc cttcagccaa tcaggacgat tgttggcggc
    12421 cgcgttggtt gggaacggga gcgtgcgacc accggataga gacttgcttc tgctagactc
    12481 acgggcagaa acactacgcc tcacgggcgt atccaccaca tctggaggac caatgagcct
    12541 tacccgcaaa gacgtagaac acgtcgcctc gcttgcccgg ctgggcctga gcgacgatga
    12601 gatcgatatg ctgcaagacc agctctcctc aatccttggc catatcgagg tcctcaacca
    12661 gctcgatact gaggcgatcc cgccgaccgc ccaggtcatt cagcttgaga acgttcttcg
    12721 tccagacgaa atgcgaccgt cactgcccca ggaaacggta gtcaccatgg cacccaaaac
    12781 acgggacggc ttcatcgccg tcgctgctgt catgggcggc aaccatgacg ggggggtggc
    12841 gtgagtcttc ccgccgattt gaccctgatg accgccgcac aagcgcgatc cctccttgat
    12901 aaccgcgaga tctcatccgt ggaactgaca cgcgcctatt tcgaacgcat cgaggaagtc
    12961 gagcccgaga tccattccta cctgcacgtc atgacggatg ttgccctcac acaggcctcc
    13021 gatgccgacc gccgaataga ggagggagat atcgatctcc tgaccggcgt gccgatcgcc
    13081 ctcaaggaca tcctctgcac gaccgatgcg ccaaccacgg ctgcgtcgaa gatcctcgag
    13141 ggatatgtct ctccctatga cgccacggtc gtcacgcgcc tgcgcgacca gggtgccgtc
    13201 ttcctcggca agaccaacac cgatgaattc gcaatgggct cctccactga aaactccgcc
    13261 tacggagaga cccacaaccc gtgggacacg agccgtgtgc cgggtggctc ctccggtgga
    13321 tcagcagccg ccatcgctgc acgggaggca accgtatcgc tgggctcgga tacgggcgga
    13381 tcggtacgtc aaccagccgg cttctgcggc atcgtcggtc ttaagcccac gtacggtcgg
    13441 gtctcccgct acggactgct cccgtttgcc agcagcctcg accagatcgg accctttgcg
    13501 cgaacagttg aggatgcggc gatcatgctg cgtgcgattg ccggcaagga ccccctcgat
    13561 tccacctctg ttcccgttga ggtgccggat tacctggcgg acttgaggca ggacttgaag
    13621 ggtgttcgca tcggcgttgc accacagtac accgtcagcg gtatggagcc cggcgttgaa
    13681 gccgcggtga aagttgccat caagcaactg gaggacttgg gcgcggagat cgtggaagtg
    13741 tcgttgccac acacggagta cgcgctggcc acctactaca tcaccgcccc cgccgaggca
    13801 agcgccaacc tggcgcgtta cgatggcgtc cgatacggca accgtgtcga gggagacaac
    13861 ctcatcgaca cctatctcaa gacacgcgga cggggattcg ggccggaggt caaacgacgc
    13921 atcatgctcg ggacgcatgc tctcagcagc ggttactacg atgcctacta tgtcaaggcc
    13981 cagaaggtcc gcacgctcat caagcaggat ttcgacgtcg cctttgaatc atgtgatgtc
    14041 gtcgtagccc ctacgtcgcc gacaatcgcc ttcaagtctg gatcccggac gagcgatccc
    14101 taccagatgt acctcgcgga catcttcacg atcccggcaa atatggtcgg tattcctggc
    14161 atctcggttc cttgcggatt cagcgacggc atgccggtgg ggcttcagtt cctcggtaaa
    14221 gccttcgacg aatcgcgctt gcttggcgtt gctcacgcat acgagcaggc cgccggttgg
    14281 tatctcaagc tgccggagat tccggcaggc tgagcgcagg agatggcctg tgacgcatcc
    14341 ccggttgcta cccccatcct tgaaaacccg ggcgggctca cttactcttg cgggtgggcc
    14401 tctagctcaa cggcagagca acggactctt aatccgcagg ttcagggttc gaatccctgg
    14461 gggcccacca tccaaaaagc ccatgatatc agggcaaaca gcctcattga tggggtcgct
    14521 gtttgccctt ttacacgccc ttgtcagagt agatg
//
LOCUS       DASYWM010000127         1867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_399157, whole genome shotgun sequence.
ACCESSION   DASYWM010000127 DASYWM010000000
VERSION     DASYWM010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399157"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VGR29_05245"
     CDS             complement(<1..1407)
                     /locus_tag="VGR29_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2073029.1"
                     /translation="MNNGTQRHSDGNVNLDRDRLTMAEAARLKGVSYHTVSRAVRKGR
                     LPVERRGRMALISARDLQAWRPMKDRAPRKYRSEHAEGNEPGSDVSLLTPDQVTLARR
                     LAALYEGIHSVASDGTIEEVAQIATEQVSEALYLKRMSLWLVDAEVNQGTRLAVVREP
                     LSNLPDTVALDGFPIFSALTEEPSARITADPLQDVALEAGYTFPSGPLLIVPLLRKNR
                     IVGVMFGDRYGDDIDFTEEQLALADGVASQVAIALDNTRLRQNERRRRLQMEAILDEL
                     ANAVSAFDEQGFLTLSNSSERALFDLSEEESRLGQHATEYLRTKFRHGLDATGTDIND
                     SPVMQVLHGERPETLDQVITRNNGETLLLRTSTRPIIAHDKIVGAISVSRDVTPEHRA
                     EIRDRQQLQQLERAAHRSQAIANLVVEINTGRDRQTVVDTALRRLTQELDADCGAIML
                     HGEDNHFYLSACRGVPPEL"
     gene            complement(1652..>1867)
                     /locus_tag="VGR29_05250"
     CDS             complement(1652..>1867)
                     /locus_tag="VGR29_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEV2073030.1"
                     /translation="IAQMPAGVPVATVAIGNARNAGLLAVRILAASDATLRSRMEQFQ
                     RQLHDMVLAKNEAVSARFNRAADQMLD"
BASE COUNT          356 a          575 c          551 g          385 t
ORIGIN      
        1 gagttccgga ggaacacctc gacaggcgga caggtagaag tgattgtcct cgccatgtag
       61 catgatagcg ccgcagtcag catccaactc ctgcgtcagc cgtctcaggg cggtgtcaac
      121 gactgtctgt cgatcccgac ccgtgttgat ctccaccacg aggttggcga tagcctggga
      181 tcggtgggcc gcgcgctcca gctgctgcag ttgctgcctg tcccgaatct cagcgcgatg
      241 ctccggggtg acgtcgcgcg agacagagat ggcaccaacg atcttgtcat gggcgatgat
      301 cgggcgtgta ctcgtcctca gcaggagtgt ctccccatta ttccgggtga tcacctggtc
      361 caaagtctca ggccgctcgc cgtggagcac ttgcatcact ggagaatcgt taatgtcggt
      421 tccagttgcg tccagaccgt gccggaactt ggtcctgagg tactcggtgg catgctggcc
      481 cagccgggat tcctcctcgc tgagatcgaa cagggcacgc tcagatgaat tggagagggt
      541 caggaacccc tgctcatcga atgccgaaac cgcgttggcg agctcatcga gaatcgcttc
      601 catctgcagc cggcgtcggc gctcgttctg acgcagcctg gtgttgtcca gggcgatagc
      661 gacctggctt gccaccccat cagcaagggc caattgctcc tcggtgaagt caatatcatc
      721 cccgtaacgg tcgccgaaca tgacgcccac gattcgattc ttgcggagga gcgggactat
      781 caaaagcggg ccagacggaa aggtgtaacc tgcttcaagt gcgacgtctt gcagcggatc
      841 ggctgtgatc cttgcactcg gctcctccgt gagcgcggag aatatcggaa acccgtccag
      901 tgccaccgta tcggggaggt tcgaaagagg ttcgcggacg actgccagac gcgtcccttg
      961 gttgacctct gcgtcgacca gccagagaga catgcgtttc agatacaatg cttcagacac
     1021 ctgctcggtc gcgatctggg ccacttcttc gatggttccg tcacttgcta ctgaatggat
     1081 gccttcatag agtgctgcca ggcgtctcgc cagcgtgacc tgatcgggag tcaggaggct
     1141 cacgtccgat ccgggctcgt tgccctcggc gtgctcactg cgatatttgc gcggtgcgcg
     1201 gtccttcatt gggcgccagg cctggagatc gcgtgccgag attagcgcca tacgcccccg
     1261 gcgttccaca ggcaaacgcc ccttccgtac cgcgcgcgag acggtatggt aggaaacacc
     1321 cttgagccgg gcggcctcgg ccatcgtaag gcgatctcta tccagattca cattcccatc
     1381 agaatggcgt tgagttccgt tgttcatgag caaccagccc ttgactggtg agacctgttg
     1441 gccatcacca ccccgaggat cacaaacagg tcttgcccgg atacaccagg atgtgtcgag
     1501 cgacaacaag tgggcagatg cacgctcctc cgtgcccacc ttgcgcagac ccgtacgttc
     1561 ctcagcacat cgcatgtgtc cacagttttg cacacctgtg gcaatgttgc tagagtaccg
     1621 gatggctctc tcaaactgcc ccgggtttac gttagtccag catctggtcg gcggcacgat
     1681 taaagcgtgc cgagaccgct tcattcttgg cgagaaccat gtcgtgaagc tggcgctgaa
     1741 actgctccat acgggagcgc agggtggcat cgctcgccgc caggatacgc acggccagca
     1801 atcccgcatt ccgggcattg ccgatcgcga ccgttgccac cggcacgccc gccggcatct
     1861 gcgcgat
//
LOCUS       DASYWM010000128         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_586723, whole genome shotgun sequence.
ACCESSION   DASYWM010000128 DASYWM010000000
VERSION     DASYWM010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586723"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1762)
                     /gene="treS"
                     /locus_tag="VGR29_05255"
     CDS             complement(<1..1762)
                     /gene="treS"
                     /locus_tag="VGR29_05255"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEV2073031.1"
                     /translation="MKDVGEPGSAAGTTWYKDAVFYEVHVRAFADSNGDGIGDFQGLI
                     GKLDYLRDLGITAIWLLPFFPSPLRDDGYDISDYTAVHPAYGNIGDFQRFLNEAHQRD
                     LKVIIELVLNHTSEQHPWFQRARRAPKGSCERNFYVWSDTPERYKQARVIFQDYEQSN
                     WSWDPVADAYYWHRFYSHQPDLNYDHPPVREAIFNSVDFWLEMGVDGLRLDAVPYLVE
                     RERTSSENLPGTHQVLKMLRNHIDTRFVGRMLLAEANQWPEDAVAYFGDGDECHMAFH
                     FPLMPRLFMALRTEDRLPVVDIMQQTPPIPDSCQWAIFLRNHDELTLEMVTEEERLYM
                     YRAFAPAEESRINLGIRRRLAPLLGNSRRRMELMNALICSLPGTPVIYYGDEIGMGDN
                     IYLGDRDGVRTPMQWSADRNAGFSAAPQQRLYNPLIVDYEYHHETVHVEAQEQNPHSL
                     LGFMKRLLALRKRHHAFGRGSLQLLQPMNHRVLAFMREHEEESILIVANLSRFSQAVE
                     LDLGAYKGRAPIEVFGRSQFWPVGAEPYRLTLGPHAFYWFDLSRPPASNAVRLGARNA
                     VAEGQALPIITTQGAWGSVVA"
     gene            complement(2107..2580)
                     /locus_tag="VGR29_05260"
     CDS             complement(2107..2580)
                     /locus_tag="VGR29_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEV2073032.1"
                     /translation="MAEESQPQYWILVSSLENWHKTVEHDFTVQGIKSRHRKKADQMK
                     PGDKFIYYCTGVKSFAGITTVQSTVFEDHSTIWLSGNKKKKEEDYPYRFRIGADLALE
                     DDNLVAAEPLARQMTYASKWAPEHWTLAFQGNVHNVPVEDYQLIRAEIEKAAEAH"
BASE COUNT          540 a          821 c          815 g          542 t
ORIGIN      
        1 cggcaacaac ggatccccag gcgccctgag tggtaatgat cggcagcgcc tggccctcgg
       61 ccacggcatt gcgtgcaccg agccgcacgg cgttcgacgc cggtgggcga ctcaggtcga
      121 accagtagaa cgcatgcggc ccaagggtga gcctgtaagg ctccgcaccg acaggccaga
      181 actgcgagcg accaaacacc tcaatcgggg cgcgtccctt gtaggcgccg agatccaact
      241 caacggcctg gctgaagcgg gagagattgg caacgatgag gatggattcc tcctcatgtt
      301 cgcgcatgaa cgccaggaca cggtgattca ttggttgcag aagctgaaga ctcccgcggc
      361 cgaacgcgtg atgccgtttg cgcagtgcaa gaaggcgctt catgaacccc aacagggagt
      421 ggggattctg ttcctgcgcc tcgacatgca ccgtctcatg gtgatactcg tagtcgacga
      481 tgagcggatt gtagaggcgc tgctggggag cagccgagaa acccgcgttg cggtccgcac
      541 tccactgcat gggggtgcgg acgccgtcgc ggtcgccaag atagatattg tcgcccatgc
      601 cgatctcgtc accgtaatag atcaccggtg tgccgggaag ggagcatatc agtgcgttca
      661 tcaactccat ccggcgtcgg ctgtttccca gaagcggagc cagccgacgc cggatgccaa
      721 gattgatgcg cgactcctcg gcaggtgcaa aagcccggta catatagagc cgctcttcct
      781 cggtcaccat ctccagcgtc aattcatcgt gattgcggag gaagatggcc cactggcagg
      841 aatccggaat cggtggtgtc tgctgcatga tatcgaccac tggcagccgg tcctcggttc
      901 ggagtgccat gaagaggcga ggcatcaacg gaaagtgaaa tgccatgtgg cactcgtcgc
      961 cgtcgccgaa gtaggcaacg gcatcctccg gccactggtt ggcctctgcc agcagcatac
     1021 ggccgacaaa gcgggtatcg atgtggtttc gcagcatctt gagcacctga tgcgtcccgg
     1081 gcaaattctc tgaagacgtg cgctcgcgtt cgacgagata gggcacggcg tccaggcgca
     1141 agccgtcaac ccccatctcc agccagaagt cgacgctatt gaagatcgcc tcgcgcacgg
     1201 gcggatgatc gtagttgagg tctggctgat ggctatagaa acgatgccaa tagtaggcat
     1261 cggcgacggg atcccacgac cagttcgact gctcgtaatc ctggaagatg acgcgggctt
     1321 gcttgtacct ctcgggcgtg tcgctccaga catagaaatt gcgctcgcag ctgcccttgg
     1381 gcgctcgtcg agcgcgctgg aaccacgggt gctgctccga ggtatggttc agcaccagct
     1441 cgatgatcac tttcaggtct cgctgatgcg cctcgttcag gaatctctga aaatcgccga
     1501 tgttgccata cgcaggatga accgcggtgt agtccgagat atcgtagcca tcgtcccgaa
     1561 gtggtgaggg aaagaagggc aagagccaga tggccgtgat gccgagatcc cggagatagt
     1621 ccagcttccc gattaagccc tgaaagtcgc cgataccatc gccgttgctg tcggcgaatg
     1681 cccgcacatg gacctcatag aacaccgcat ccttgtacca ggtcgttccg gctgcacttc
     1741 cgggttctcc aacgtctttc atgtcactac cttgtggcgc ctgacccctg tgctgtggat
     1801 gggagtatgg catggacgga caagcgctca gccgcggcgt tctctcgcca cggctccccg
     1861 agacgcggat ggtcctgcca ccggggccgg ttacggtcga gcgcatactg ctggaagccc
     1921 gatatccctg cttcaccgtc agggtctcgc cggctcaccc cgtgagatcg gggcggcgtg
     1981 gcgcgggatc cgacacaaag aggagcttgc cctccatgac gccactggtc gccacagccg
     2041 ggtgaaagac cacgcttctc cccttactcg ctgaggaatg agtagtaaca acgcacggca
     2101 ttgccgctaa tgcgcctcgg ccgccttctc gatctcggcg cggataagct ggtaatcctc
     2161 cacaggaacg ttgtggacgt ttccctgaaa tgcaagcgtc cagtgctcgg gcgcccattt
     2221 cgaggcatag gtcatctgcc gtgcgagcgg ctcggcggcg accagattgt catcctcgag
     2281 cgccagatcg gccccaatcc ggaagcggta aggataatcc tcttccttct ttttcttgtt
     2341 gccgctgagc cagatagtcg agtggtcctc gaagacggtc gattgcaccg ttgtgatacc
     2401 cgcgaaggac ttcactccgg tgcagtagta gatgaacttg tccccgggct tcatctgatc
     2461 cgccttcttg cgatggcgcg acttgatccc ctgcacggtg aaatcgtgct cgacggtctt
     2521 gtgccagttc tccaacgaac tgacgaggat ccagtactgc ggttgcgact cctcggccac
     2581 gtgggtgctg ctccttccat tagtagtaga tagccgtgtc ctgccagaga caccatacgg
     2641 ttcgcattat cgttcacctc gcgtcccgct ggagctcact cagacgggag gctgcgtcag
     2701 catccagcca ccatccac
//
LOCUS       DASYWM010000129        12547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_211434, whole genome shotgun sequence.
ACCESSION   DASYWM010000129 DASYWM010000000
VERSION     DASYWM010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12547
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211434"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /gene="iolG"
                     /locus_tag="VGR29_05265"
     CDS             <1..899
                     /gene="iolG"
                     /locus_tag="VGR29_05265"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEV2073033.1"
                     /translation="QVTARLLGVPRATADSEAVIADPDIDAVIIASSTETHAPYIELA
                     ASHGKDIFTEKPIALDLETTQRALAAVERAGVRLQVGFQRRFDAGYVAAKQAIEGAEL
                     GAIEMIRDAMRDPEPPPTDYIKRSGGLYRDMTVHNFDCVRWLMGEDPIELHATASTLI
                     SDEFREANDFDTSIVTLRFPSGALASIENSRRSGFGYDVRTEIFGSEGALMVGESRAL
                     PVRRLSASGVLEDHQYFFLERFRDAYREELVAFLTALRDDTPVAVTGEDGRAALVLAY
                     AAERSLLEHRPVPIDLKEVSYA"
     gene            892..1791
                     /gene="iolB"
                     /locus_tag="VGR29_05270"
     CDS             892..1791
                     /gene="iolB"
                     /locus_tag="VGR29_05270"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEV2073034.1"
                     /translation="MPDTEKMLIHPELPEGTGRVLEVTPESAGWEYVGLTVQKVAPGG
                     VWNFSSPDDEICIVPLRGSAVITDGKEEWTVSRPGTIFDGKPTALYLPVGSSFKATSD
                     DGAELAVTSSRATRTFPAKLIEPDDIDVEIRGAGNAARQISHIIKPGFPADKLLVVEV
                     FTPSGNWSSFPPHKHDVSNMPEEADLEEIYYYRIQPNDGFGLQRLYYADGSFDHAWVI
                     KDGDLLLVPEGYHAFAVAHGYTGYYLNILAGDEPERTMQPADDPAYAWVRSTWNDAMN
                     EGISSWRDIDDRVNGDAGKRKDL"
     gene            1797..2993
                     /locus_tag="VGR29_05275"
     CDS             1797..2993
                     /locus_tag="VGR29_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV2073035.1"
                     /translation="MIRVTAAQAVVRFLAAQYTERDGVEQPLFAGMFGIFGHGNVTGI
                     GQALEEHRGLLTYYRSQNEQAMVHTAVAYAKMKNRLQTFACTSSVGPGATNMVTGAAL
                     ATVNRLPVLLLPGDIFASRRPHPVLQQLEFPGSQDVSVNDAFRPVSRYFDRIYRPEQL
                     FSALPEAIRVLTDPAETGAVTLAMPEDVQTEAFDVPESFFRKRVWHVRRPTPPEEVLI
                     EAARLIAASEKPLIISGGGTIYSDGSEALDEFATQCGIPVSETQAGKGALPWDHPWNV
                     GPIGANGSVAANRIAKDADLVIAVGTRLADFVTSSKAAFQNPDVRFIGINVSSMDAHK
                     MGAVSLIGDARATLSALQKLVLEEGITRNVTDLASQVQVLQQEWNSLVDTQRALDTML
                     RQPRPR"
     gene            2999..3664
                     /locus_tag="VGR29_05280"
     CDS             2999..3664
                     /locus_tag="VGR29_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV2073036.1"
                     /translation="MVNDASNPQDVVVCAAGGMPGDLLKLWRTEDPKGYHVEYGYSCM
                     GYEIAGGLGVKMAMPDREVFIMVGDGSYLMLHTEIVTAVQEGQKLIIVLVDNSGFQCI
                     RGLQMNSGSPSFGNELRFREDTTNALTGAFVPVDFAKNAESLGARAIAATTPDEFRSA
                     LEEAKAESRTTLIYVRVDTEGRVPNYEGWWDVPIAEVSDEESVREARAQYEEDIQKQR
                     LFV"
     gene            complement(3728..4630)
                     /locus_tag="VGR29_05285"
     CDS             complement(3728..4630)
                     /locus_tag="VGR29_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073037.1"
                     /translation="MINMSPDVATDVSAAAESAPSTRRRFWRRLRSERKVLIGLVIVA
                     ILAFIAIFGPLLAPYDPEADDYELFAAPSLDHLFGTDSFGRDILSRMMAGTRISFTVG
                     ISVAVLSMVVGVTLGLIAGYFGGWLDSLIMRFMDLLWAFPEIILAVGMVAVFGAGIRN
                     IIIAIAVAYLDDFARVVRAEVLRLREEEYALAAKAIGASHRRIMLMHILPNTLAPVIV
                     QATFAVGLGILAESGLTFLGLGVSPSTPTWGLTLNEGRDFIRQAWWISVFPGLAIMVT
                     VLGLNLLGDGLRDTLDVRGVKETV"
     gene            complement(4623..5570)
                     /locus_tag="VGR29_05290"
     CDS             complement(4623..5570)
                     /locus_tag="VGR29_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073038.1"
                     /translation="MTQYIIRRLIHSVIIVWGCATLVFFLMRAIPGDPIIQMLGPEYT
                     PEAADALRRKLGLDEHVLIQYVQWMGDILRGDLGSSITGAESVRSAILSGLPKTMSIA
                     ALSFVIATTIAVPAGILAALKRNSFADFAVSLFAFLGVSMPSFWFGIILILVFAVNLG
                     WLPSLGYASISDDGLLEWFKHLILPSLAIGAGYAAILMRFVRAGLLEAMGSDYIRTAR
                     AKGLAEKRVIIGHGLRNSLIPVVTVAGIQVALLLSGTVVIETVFAIRGIGRLLVGAIF
                     DKDYPMVQGAILVVAVIFVFANLLVDILYTLIDPRIKYD"
     gene            complement(5640..6842)
                     /locus_tag="VGR29_05295"
     CDS             complement(5640..6842)
                     /locus_tag="VGR29_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2073039.1"
                     /translation="MYTYERTIDPDFASPQANKLALITDITAPDDTTLVIKQSAPYAP
                     FLATACSRGPGRALAPVPQRAVEEMGDEQFGQAPVGCGPFMIVPETVETGGGFEMVAF
                     ENWYAGRPLLDKVTVQLVAEPSTLVSALEAGDVDMVDIVPLIGYEQISSVEDITMVEA
                     AGTNWYGLTMNYNRPPWDNLDARMAVVKAIDFDDLNKKAFFGRAIRSIGPLAPAFGWV
                     YVPADEVDSPQTFNLDEAKQLAQKAGLEGVQPQLIGTPADQRVTETIRSQLSEIGLDV
                     QVDQMQTNAYVERRTAGDFDMTMLGSVVDADPDDGVWNYFHSTGPSNPEGYNNPEADR
                     LVDAQRETADEAERTRLLQELQTLVANDAVYRFFYHLPDVTAFYNYVQGYVPVPEQRY
                     LETVWLDQ"
     gene            complement(6818..7288)
                     /locus_tag="VGR29_05300"
     CDS             complement(6818..7288)
                     /locus_tag="VGR29_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2073040.1"
                     /translation="MKDRGTINWSVLSGPRVSRRTLLQLAAASGATAYAAQLSNAAAS
                     SSTTQRVMGALRQEAKQGGELRWGFGLGQIPTLDPAQVNLGIVAGELLSNLFSGLVQF
                     DQELGIIPDLAEDWEVTEDGLQYTFNLRSGLTFHNDDPLTANDFIYTYIHTSEP"
     gene            complement(7737..8891)
                     /locus_tag="VGR29_05305"
     CDS             complement(7737..8891)
                     /locus_tag="VGR29_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEV2073041.1"
                     /translation="MRITDVKTFIVYAPERGRNFTFVKVYTDQGLTGLGEAGVTGKEL
                     AIEGLIATYREFLIGMDPSRIEHIWQTLWRGQFFRGGHIHGAAVAAIDLALWDLRGKA
                     LGVPVYDLLGGKTRDHARCYCHVQQPGGIDAMVASARERVADGWEFLRYGVGEGSEPE
                     RDGIHEQSKAVQWSIDAFAALRDAVGPDIEICVDFHQRTTPAYAIQLARALEPMRPFF
                     VEDPVRAENASQFSYLRQHIDVPIATGEQLASKWDWQPLIEGDLMDYCRIDLCIGGGL
                     TEARKVAGWCETHYIEQVPHNPLGPVSTAACLHFDLSTPLFAVQELVWRSDVMSNIVP
                     TDMRLEGGNLFSGGTPGLGVELDEEAALAHPYQPQGLMALRREDNSVSDW"
     gene            9388..9606
                     /locus_tag="VGR29_05310"
     CDS             9388..9606
                     /locus_tag="VGR29_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073042.1"
                     /translation="MANDNSAGTRDVTYNLISVVYHALQGADTYHTYVRDAETEGDEE
                     LTGFFREAEQQSEQLAARAKELLGQRMK"
     gene            complement(9795..12248)
                     /locus_tag="VGR29_05315"
     CDS             complement(9795..12248)
                     /locus_tag="VGR29_05315"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV2073043.1"
                     /translation="MLIGPGDGRAIAVFLFMVALVAWNRFAFDSWLSRFDIYTQFLPW
                     YDYLGDRTREFDVPGWNPHMLSGTPFAGHPLSGWMYLPAMIAFALFPVITAFKVLIVS
                     HLVIAGLGVYALARVLGMGPLAGLVGAVVFAFGPFLEWNTYTSLQFAQFTVWVPLVLL
                     GIELALRTPQWRGRLVPWCVAGIGISQMFAGWVGEGWIYAALITGVYAGYRAVFAPWQ
                     SSLGMRHRLIAGVATGTAALGSGMLLGAAGILPRFAVNAQTNLAGGAYADLEGGGSLN
                     SPWDVGYLLTQIMGMGTGYHHRAAGLGGAIVVLMLLALLLARSSFAVPFFAGLTSVAL
                     ILTLEANPVHYFFYLIPGYRNLHEHDPWRVVALASIGPAMLSAAAVEVLGQLRGRYRI
                     LPVVVALPLGVMAIDTVLGPVEGVAGWAPIIAAAAITELLVVIIAVPRDRDLRLPLRW
                     IPQAALALVVAVIFIQPTGLELTGSWFGWPEDKRWERRWNPDAVTEQAREIEVRSEDP
                     GGAGEYLQNQLDDSGPFRYLGYGGVGHPDGGWNARSYMDRRFDSYAQAILVNGRPMFL
                     GLYEIQGYDPVQLARYVEYVAAINGKGQDYHTAFVSDEGVGSPLISLLHVRYILVDAS
                     LPQDRADVEALTSGYEEVFRTNFVVVHQRQDPVPHAWIVHDVRPVKRGDALPLIVEGE
                     VDPYRTALVEGDTPDIEAVPAASGDQAIVTQYDAEKVTIETHSDAPGFLVVSEVYADG
                     WRAFVNGRETEILPTHHALRGVPIPAGDATVEMRYEPLALRVGIPVSIGTAVVMLSVF
                     ALGGWRSRWASQVVTAERK"
BASE COUNT         2744 a         3840 c         3596 g         2367 t
ORIGIN      
        1 cacaggtcac agccaggttg ctgggcgtcc cgagagctac ggcggatagt gaggcagtga
       61 tagccgatcc ggacatcgac gcggtcatca tcgcgtcgtc gacggagacg catgctcctt
      121 atatcgagct cgcggcttcg cacggcaagg acatcttcac ggagaagccg attgccctcg
      181 acctcgagac gacgcagcgg gcgctggcag cggtcgagcg agcaggtgtg cggctccagg
      241 tggggttcca gcgacgtttt gacgctggct atgtcgccgc gaagcaggcc atcgaaggcg
      301 ccgaactggg cgcgatcgag atgattcggg atgcgatgcg tgacccagag ccgccaccaa
      361 ccgactacat caaacgatct ggcggcctgt atcgcgacat gacggttcat aatttcgatt
      421 gtgtccgctg gctcatgggt gaagacccga ttgagctgca tgccaccgcc agcacactga
      481 ttagtgatga gtttcgcgag gcgaacgatt ttgataccag catcgtcacc ctccgattcc
      541 cgtcgggagc actcgcctcg attgaaaaca gccgcagatc gggcttcggc tacgatgtcc
      601 gcaccgaaat tttcgggtca gaaggtgccc taatggtcgg tgagtcccgg gccctgccgg
      661 ttcgccgcct ctcagcgtct ggcgtgctgg aagaccatca gtacttcttc cttgaacgct
      721 tccgcgacgc ctaccgggag gaactggtcg cgttccttac ggcactacgc gatgacactc
      781 ccgttgcggt caccggagag gacggccgcg cggccctcgt gctcgcctat gctgcagagc
      841 gctcgctcct cgaacaccgg ccggtgccaa tcgacctgaa ggaggtttcc tatgcctgac
      901 accgagaaga tgctgatcca tccagagtta cccgaaggga ctggtcgtgt gcttgaggta
      961 acccctgaaa gcgccggctg ggaatacgtc ggtttgaccg tccagaaggt cgcaccggga
     1021 ggcgtctgga acttctcatc acccgacgac gaaatctgca tcgtacctct tcgcggctcg
     1081 gcagtcatca ccgacggcaa ggaggagtgg accgtctccc gacctggcac catattcgac
     1141 ggaaagccga cggccctcta tctaccagtg ggcagtagct tcaaggcaac gtctgatgac
     1201 ggcgccgagc tcgccgtgac gtcgtcccgg gcgacgcgca cgttcccggc aaagctgatc
     1261 gagccggatg atatcgatgt cgagatccgt ggcgccggga acgctgcccg ccaaatcagc
     1321 catataatca agcccgggtt tcccgccgat aaacttctcg tcgtcgaagt gttcacacca
     1381 tcaggcaact ggtcaagctt cccgccgcac aagcacgatg tgagtaacat gccggaggaa
     1441 gcggatctgg aggagatcta ctattatcgg atccagccga acgacggctt cggcctgcag
     1501 cggctttact acgccgatgg ctccttcgat catgcctggg tgatcaagga cggcgatctc
     1561 ctgctggtcc ccgagggata ccatgccttc gccgtcgccc atggttacac ggggtactac
     1621 ctcaacatcc tcgccggcga cgaaccagag cggaccatgc agcccgcgga cgaccctgcc
     1681 tacgcatggg tacgatccac gtggaacgat gccatgaacg agggaatttc cagttggcgt
     1741 gatatcgatg accgggtcaa tggggacgcg ggcaagcgaa aggacctttg atcgtcatga
     1801 ttcgcgtcac cgccgcacag gcagtcgtgc gctttctggc tgcccagtac accgagcggg
     1861 acggcgttga gcagcccctc tttgctggca tgttcggcat cttcgggcac ggcaatgtga
     1921 ccggtatcgg acaggctctt gaggagcatc ggggcctcct cacctactac cgatcacaaa
     1981 acgagcaggc gatggtccat accgcggtcg cctacgcgaa gatgaagaac aggctccaga
     2041 cgtttgcctg cacctcaagc gtgggacccg gcgccacgaa catggtgacg ggcgccgccc
     2101 tcgcgacggt aaaccgcttg cctgtcctgc tccttccggg ggacatcttt gcaagtcgcc
     2161 gtccccaccc cgtgctgcag cagctggaat ttcctggatc gcaggatgtg agcgtcaacg
     2221 acgccttccg gcccgtgtcg aggtatttcg atcggatcta ccgcccggaa cagctcttct
     2281 ccgcgttgcc tgaagcgata cgcgttctta ccgatccggc agaaaccggg gccgtcaccc
     2341 tcgctatgcc cgaggatgtc cagactgagg ccttcgacgt gccggaatcc tttttcagga
     2401 agcgggtctg gcacgtgcgg cgcccgacac ctcctgaaga ggttctgatc gaggcggcgc
     2461 gcttgatcgc cgcgtcagag aagccgctca tcatcagcgg tggcggtacc atttactctg
     2521 acggcagcga agcgctcgac gaattcgcca cccagtgcgg aatccccgtc tccgagaccc
     2581 aggcagggaa aggcgctcta ccatgggacc acccctggaa cgtcggacca atcggcgcaa
     2641 atggtagtgt cgccgcaaac cgcattgcga aggatgccga tctcgtcatc gccgtaggca
     2701 cacggctcgc agatttcgtc acctcctcga aagcggcatt ccagaatcct gacgtccgct
     2761 tcatcggcat caatgtctca agcatggacg cgcacaagat gggtgccgta tcgctgattg
     2821 gagatgcacg agcgacgctt tcggcactcc agaaactcgt gctcgaggaa ggcatcaccc
     2881 gaaacgtcac ggatctcgca tcccaggtcc aggtccttca gcaggaatgg aacagtcttg
     2941 tcgacaccca gcgggcactg gacacaatgc tgagacaacc caggcccagg taatcggcat
     3001 ggtgaacgac gcctcgaatc ctcaggatgt cgtggtctgt gccgcaggtg gcatgccggg
     3061 cgacctcctt aagctctggc gcaccgagga tcccaagggg taccacgtcg aatacggcta
     3121 ttcatgcatg gggtacgaga tcgccggcgg actaggcgtg aagatggcca tgccggaccg
     3181 cgaggtcttc atcatggtcg gcgacggtag ctacctgatg cttcacacgg agatcgtcac
     3241 ggcggtccag gaggggcaga agctgattat cgtcctcgtg gacaacagtg gattccagtg
     3301 catccgcggc cttcagatga attctggctc tccctcgttc ggcaatgagc tccgcttccg
     3361 ggaggacaca accaacgccc tcactggtgc ctttgtgccg gtcgatttcg cgaagaacgc
     3421 cgaaagcctc ggcgcccggg cgatcgccgc cacaacgccg gacgagttcc gatccgcact
     3481 ggaggaagca aaggcggaat cgcggacgac cctcatctac gtccgcgtcg acaccgaagg
     3541 gcgggtaccg aactatgagg gctggtggga tgtcccgatc gccgaagtga gcgatgagga
     3601 atcggtgcgg gaggctcgcg cccagtacga ggaagatatt cagaagcaac gtctcttcgt
     3661 ctgatccttg tcgcaacgca gaagggcctg ggcggatccg ccccggccct tctgagcgtt
     3721 cgtccggtta gacggtctct ttgactccac ggacgtctag tgtgtcacgc aaaccatcgc
     3781 cgaggagatt gaggccgagc accgtcacca taatcgcaag accagggaaa acactgatcc
     3841 accaagcctg acggatgaaa tcccttccct cgttcagggt gagcccccag gtcggtgttg
     3901 aaggtgagac gccaaggcca agaaacgtga ggccggattc cgcaaggatg cccagcccaa
     3961 ccgcgaaggt cgcctggacg atgaccggtg caagcgtatt cgggaggata tgcatcaaca
     4021 tgatgcgacg gtgagatgca ccgatcgcct tcgccgccag cgcatactct tcttcccgca
     4081 accgtagcac ctcggcacgc accactcgcg caaagtcgtc aagataggca acagcaatgg
     4141 cgatgatgat attgcgaata ccggccccaa ataccgcaac catgccgacg gcgaggatga
     4201 tttcaggaaa cgcccacagc aggtccatga atcgcatgat gagagaatcc agccatcccc
     4261 cgaaatatcc tgcaatgagc ccgagcgtga ccccaactac catggagagc accgcaacag
     4321 agatcccgac ggtaaacgag atccgtgttc cggccatcat cctgctcagg atgtcacggc
     4381 caaaagaatc cgttccgaac agatgatcca ggctcggagc agcaaagagc tcatagtcgt
     4441 cagcttcagg atcatatggc gcgagcaggg gcccaaaaat cgctatgaac gctaggatag
     4501 cgacgatgac cagaccgatc aacaccttgc gctcgcttcg cagccggcgc cagaagcggc
     4561 gccgggtcga tggcgcagac tctgcagcag ccgatacatc cgttgcgaca tccggggaca
     4621 tattaatcat atttgatcct gggatcgatg agcgtataga ggatgtcaac gagcaggttc
     4681 gcaaatacga agatgacggc tacgacgagg atggcaccct gaaccatggg atagtccttg
     4741 tcgaagatgg caccaaccag taatcggcca attccgcgaa tggcgaagac tgtctcgatg
     4801 acgacggttc cactcaggag caaggcgacc tgaatcccgg caaccgtcac aacggggata
     4861 agcgaattac gcagcccgtg cccgatgatg acccgcttct ccgccagccc cttggcccgg
     4921 gcagtacgaa tgtaatcgct gcccatcgcc tcgagaagtc ccgcccgcac gaaccgcata
     4981 agaatcgcgg cgtatccggc accaatggcc aggctcggca agatgaggtg tttaaaccat
     5041 tccagcaatc cgtcatcgct gatggaggca taacctaggg agggcagcca cccgaggttc
     5101 acggcgaaga ccaggatcag gatgatgccg aaccagaaac tcggcatgct gacaccgagg
     5161 aacgcgaaaa gacttaccgc gaaatcagcg aaagagttcc gcttcagcgc cgccaaaata
     5221 ccagcgggga ccgcgatcgt cgtcgcgatg acaaaagaaa gcgcagcgat actcatggtc
     5281 ttggggaggc ctgaaagaat cgcactccgc acgctctccg cgccggtaat cgaagaaccc
     5341 aaatctccgc gcaggatgtc acccatccat tggacgtact ggatcagtac atgctcatcg
     5401 agtcccagtt tgcgacggag ggcgtccgct gcttccgggg tgtactcggg accgagcatc
     5461 tggatgattg gatcgcccgg aattgccctc atcaggaaga agaccagggt cgcacagccc
     5521 cagacaatga tgacgctatg gatcagccgg cggatgatgt attgcgtcaa gtgaccgcct
     5581 ccccacgttc tgcctggcca gcaatgacca ggcagaacgc cccacgaggt ttccggattt
     5641 tactgatcga gccagaccgt ctccaggtag cgctgctcgg gaacgggaac gtagccctga
     5701 acatagttgt aaaacgcagt cacatcgggc agatggtaga agaaccggta tacggcatcg
     5761 ttggcgacaa gcgtctggag ttcctgaaga agacgcgttc gttccgcttc gtccgcagtt
     5821 tccctctgtg cgtcgacgag cctgtcggct tccggattgt tgtacccctc aggattggat
     5881 ggaccagtcg agtggaagta gttccacacg ccgtcgtccg gatcggcatc aaccacacta
     5941 cccagcattg tcatgtcgaa gtcacctgct gtgcggcgtt cgacataggc gttggtctgc
     6001 atctggtcca cctgcacgtc caggccgatt tcactaagtt ggctacggat tgtctccgtc
     6061 actcgctggt ccgccggagt cccgatgagc tggggctgta ctccctcaag gcctgctttc
     6121 tgggccagtt gcttggcttc atcaagattg aaggtttggg ggctatccac ctcatctgcc
     6181 gggacataca cccagccgaa cgcaggtgcg agggggccga tacttcggat agctcgaccg
     6241 aaaaaggcct tcttgttcag gtcatcgaaa tcgatagcct tcacaactgc catccgggcg
     6301 tcgagattgt cccatggcgg ccggttgtaa ttcatcgtca ggccgtacca gttagttccc
     6361 gcggcctcaa ccatggtgat gtcctcgacc gaggagattt gctcataccc aatgagcggc
     6421 acgatgtcca ccatgtcgac atcgccggct tccagcgcgc ttacgagcgt cgaaggctct
     6481 gccaccaact ggaccgttac cttatccaat agtggtcgtc cggcgtacca gttctcgaaa
     6541 gccaccatct cgaaaccgcc acccgtctca acggtttcag ggacgatcat gaagggaccg
     6601 catccgaccg gagcctgccc aaattgctcg tcgcccattt cttcaacggc gcgttgcggg
     6661 actggcgcga gtgcacgccc gggtccccgg ctacaggccg tagcaaggaa aggtgcgtag
     6721 ggggcgcttt gcttgatgac cagggtggtg tcgtcaggtg cggtgatatc tgtgatcagg
     6781 gccagcttat tggcctgtgg tgaggcaaag tcgggatcta tggttcgctc gtatgtatat
     6841 atgtatagat gaaatcgttg gcggtaagcg gatcatcgtt gtggaaagtg agaccggagc
     6901 gcaggttgaa cgtgtattgg agtccgtcct cagttacttc ccagtcctcg gcgagatctg
     6961 gaataatccc cagctcctga tcaaactgca caaggccaga aaagaggttg gacagcagct
     7021 caccggcgac gatcccaagg ttcacctgtg ccgggtcaag cgttggaatc tggccgagtc
     7081 cgaatcccca acgtagttca ccgccctgtt tggcttcctg gcggagcgcg cccattactc
     7141 gctgcgtcgt tgaacttgag gccgcggcat tcgacagctg tgccgcgtat gccgtcgcgc
     7201 cgcttgcggc ggcaagctgt aacagggtac gccgactcac ccgtggtcca ctgagaacgc
     7261 tccagttgat cgtgccgcgg tccttcatct atagactcct ttgcgggact gccacgtggc
     7321 atccgatacg aggcgtatcc atgagattgg tgtagaaagc agtctcacat ggggtaaaaa
     7381 tattgtcaat gaactgcacc gggacacaaa cctcatcagt cgtggacctg atctgaaaca
     7441 ggcattcttc tccgatcggt gatcgctcct aatcacagct gcccatgacg taccacctcc
     7501 tggactccga taccctacgc actggttcga gccgaccata gaccctctcc atgtagtttt
     7561 tcctgcgtag ccgacgtaag cgctttcaac ttctgcagga acgtgcatgg cggagccagc
     7621 gattgtccac gtcatgagtg gcttccactg cctcgggacg aaacatcttc cctccgctcc
     7681 cactgcggtc cttaagccga aatccatgtg cgttctgctc aacgtcccat gcatgttcac
     7741 cagtccgaca ccgagttgtc ctcccgtcgg agcgccatca agccctgggg ttgatacgga
     7801 tgggccaaag ccgcctcttc atccagttcc acaccgagac ccggtgtgcc accactgaag
     7861 agattgccgc cctcaagcct catatcagtc ggcacgatgt tggacatgac gtcggatcgc
     7921 caaaccagtt cctggacagc aaagaggggg gtagaaaggt caaagtggag acatgccgca
     7981 gtggagactg gtcctaaagg attgtgcggg acttgctcaa tgtaatgtgt ctcgcaccaa
     8041 ccggcgacct tcctcgcctc ggtcaacccg ccaccaatac acaggtcaat gcggcagtaa
     8101 tccatcaggt cgccctcgat gagtggctgc cagtcccact tgctggccaa ctgctcacct
     8161 gtcgcgatgg ggacgtcgat atgctgccgg agatacgaga actgcgatgc gttttctgcc
     8221 cgcacaggat cctcgacgaa aaacggccgc atcggctcga gcgctcgcgc caactggatc
     8281 gcgtaggcgg gcgtggttcg ttgatgaaaa tcgacacaga tctcgatatc cggcccgacg
     8341 gcatcgcgca gggcggcaaa ggcatcgatg ctccactgga cggctttcga ctgctcatgg
     8401 atgccgtctc gctccggctc actaccctca ccaacaccgt aacgaaggaa ttcccaccca
     8461 tccgcgaccc gctctctggc ggaggccacc atggcgtcga ttcctccggg ctgctgcacg
     8521 tggcaatagc accgggcgtg gtcccgagtc ttcccgccga gcaagtcata cacgggcacg
     8581 ccgagtgcct tgccccggag atcccaaagc gctaggtcga tggcggcaac agccgcccca
     8641 tggatatgac caccgcgaaa gaattgccct cgccagagtg tctgccagat atgttcgatt
     8701 cgggacgggt ccataccgat caggaattcc cggtacgtgg ctatcaatcc ctcgatagcc
     8761 agttccttgc ctgtcactcc cgcttcgcca agtcctgtca gtccctggtc ggtataaacc
     8821 ttcacgaagg tgaagttccg ccccctctca ggagcgtaga cgataaacgt cttgacgtct
     8881 gtaatcctca tcttgtctcc ccttctgaca ggcaatcccc aagattggta gcgcgagcct
     8941 gtcgacgcag ctcccggctc gacttcggta gcatcagctg ttgcctaggc gattttccgg
     9001 cagcagcgcg gtgacaactg taaatctgga ccggttgcaa aaaggcaaaa tgactttcgc
     9061 gattgtcgcc gtgaggatcg aggaggtcaa cgccgccgag agtccctgta ccacagggaa
     9121 cgggtggcag ggagccgctc gaaggccatg taatgaattc gcccaggccg ccggtcactc
     9181 ccaaggctat gcggcgtggg acgggatgac gcacagccgg aacgaccttc gcttgaatct
     9241 tcacccggtt gcccaatctc cccaaacatc tgccccatat acacgggtac aacctcagtt
     9301 agaggtggcc cctttcttgc gcctataacg tcgagagtcg cccacctgtt gttgcggtga
     9361 ccgacgatga gaaggggaga tccgttcatg gccaatgaca actctgccgg aacacgagac
     9421 gttacctaca atctgatcag cgtcgtctat cacgccctcc agggcgcgga cacgtaccat
     9481 acctatgttc gcgatgcaga gacggaaggc gacgaagagc tcaccggatt cttccgcgaa
     9541 gccgagcagc agagtgagca actggccgcc cgtgcgaagg agctactcgg gcagcgaatg
     9601 aagtagaatc gcgacccttg aagtccttga gggcgcgctt tatatagaca ttgacattga
     9661 aagaacggcc ggcttcgcga acgcgaagcc ggccgttcag tttcgcccct tgtcctacat
     9721 tttccgctga atcaactgcg gaggtcatgc gttgtggaag caaagcgccg accgcgatca
     9781 gcgccggaaa gcgatcattt tctctctgca gtcactacct gggacgccca cctactcctc
     9841 cacccgccca acgcgaacac gctgagcatt accacagcgg ttcctatgct cactggtatc
     9901 ccgacgcgca atgccaaggg ttcgtaacgc atctctaccg tggcatcccc tgcaggaatc
     9961 ggcacaccgc gcagggcgtg gtgggtcggc agaatctccg tctctctgcc attgacaaag
    10021 gcacgccaac cgtccgcgta gacctcactg actaccagga atcccggcgc atccgagtgc
    10081 gtctcgattg tgaccttttc cgcgtcgtac tgcgttacaa tcgcctgatc gccgctggcg
    10141 gcgggtacgg cctcaatatc gggagtatcc ccctcgacca atgcggtcct gtacggatcg
    10201 acctccccct ctacgatcag cggtagggca tcaccccgct tgactgggcg aacatcgtgg
    10261 acgatccatg catgagggac cgggtcctga cgctgatgga cgacaacaaa gttagtgcga
    10321 aagacttcct cgtatccgga tgtaagggcc tccacgtctg ccctgtcctg cggcagcgaa
    10381 gcatctacga gaatgtatcg aacatggagc aggctgataa gcggagatcc gaccccttca
    10441 tcagaaacga aggccgtgtg gtagtcctgc cccttgccat tgatcgcggc aacatattcc
    10501 acgtatcggg caagctgaac cgggtcatat ccctgtatct cgtagagacc gagaaacatc
    10561 ggtcggccgt tgaccaggat agcctgggca tacgagtcaa aacggcggtc catgtaggac
    10621 cttgcattcc atccgccatc ggggtgtcca actccaccat acccgaggta acgaaagggg
    10681 ccggaatcgt cgagctgatt ctggaggtac tcgccggctc cgccagggtc ctcactcctg
    10741 acctcgattt ccctcgcctg ctcggtcact gcgtccggat tccagcgtcg ttcccacctc
    10801 ttgtcctccg gccatccaaa ccatgaaccg gtcaactcaa gccccgttgg ctggataaag
    10861 attacagcga cgacaagtgc taacgctgcc tggggaatcc accttaatgg cagccggaga
    10921 tctcggtctc tgggaaccgc gataatgacc acgaggagtt cagttatcgc tgcggcagca
    10981 atgatcgggg cccagcccgc cacaccctcc acagggccaa gaacagtatc gatcgccatc
    11041 acccctagtg gaagcgcgac cacaaccgga aggatgcgat acctgccgcg tagttgcccc
    11101 aacacttcca cggcagcggc gctcaacatg gcgggaccga tcgatgcgag ggcgacaaca
    11161 cgccagggat catgctcgtg cagatttcgg tagccgggga tgaggtagaa gaaatagtgg
    11221 accggattcg cctcgagcgt caggatcagt gccacgctcg tcagtccagc gaaaaagggg
    11281 acagcgaagc tgctgcgagc gaggagtagc gccaggagca tcaggacgac gatcgctccg
    11341 cccagacctg ctgcccggtg gtgatacccg gtccccatcc ccataatctg ggtgaggagg
    11401 tagccgacat cccacggcga gttgagggag ccacctccct ccaggtcggc ataggccccc
    11461 ccggccaggt tagtttgcgc attcacagca aaccgaggca ggattccagc ggcacccagc
    11521 aacattccac ttcccagcgc ggctgtgccg gtggcgacgc cagcgataag ccggtgcctc
    11581 attcctagac tggattgcca tggagcgaag accgctcgat acccggcata aacaccggtg
    11641 ataagcgccg catagatcca tccctcgccg acccagccgg caaacatctg gctgatcccg
    11701 atgccggcca cacaccacgg gacgagccga cctcgccatt gtggtgtccg gagcgcgagt
    11761 tcgattccga gcaacactag aggaacccac acggtgaact gggcgaactg cagtgacgta
    11821 tacgtgttcc attcgaggaa gggcccgaag gcgaagacca ccgcaccgac aaggcctgcg
    11881 agcggcccca tcccgagtac ccgggcaagt gcatagacac ctagacctgc gatcacgagg
    11941 tggctaacaa tcaagacctt gaatgcagtg atgacgggga aaagcgcaaa agcgatcatc
    12001 gccggcaggt acatccagcc ggaaagggga tggccggcga aaggggtgcc gctgagcatg
    12061 tgcgggttcc atcccggcac atcgaattcc ctggtccggt ccccaagata gtcataccag
    12121 ggcaggaact gggtatagat gtcgaatcgg gatagccagg agtcgaaggc gaatcggttc
    12181 caggcgacaa gcgcaaccat aaacaggaaa acggcaattg cccgtccatc gccagggcct
    12241 atcaacacgt tggcgcattc tcgtcgtcgg tccggattca acaagaccgg tgcatcaggc
    12301 tgcattattc gtcatggtgt ccggtattgt gacggctcaa gggccgttgc ctacggtggg
    12361 caccagccgc ccacttcact gccagtgcaa gggcacaatc actgccacgc tcgatggcag
    12421 catccgaatg caccgatcga tgaaagagtc ggaaaagctc ggaatatgct ggtgccgaac
    12481 ctgcttcggc agcgggaaga cacaagaacg gtcacggctc aggagccacg accgtttctc
    12541 tccagcg
//
LOCUS       DASYWM010000130         6114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_23334, whole genome shotgun sequence.
ACCESSION   DASYWM010000130 DASYWM010000000
VERSION     DASYWM010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23334"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1726)
                     /locus_tag="VGR29_05320"
     CDS             complement(146..1726)
                     /locus_tag="VGR29_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073044.1"
                     /translation="MNLRHDAAERIDFRNILSSRALTAGLICVFLGAIDLTVIASILS
                     TIIPDLGVNTADIDRYIWVVNGYLIAYIVAIPLVGRLSDVFGRQAAFFACLAIFAVGS
                     ILCATAESLSELVAGRTIQGLGGGGLLPVTIALASDVLPRRMRLAGIGLVSAVDTLGW
                     VLGPTWGAVIVGLLPGTDERWRWVFWLNLPFLLVALVAILKGFPHRSHLRKREAARAV
                     DVPGALLLAIALICVNLALASGGEFGAKAGSGLRALGGTPNPFADHIPLLLGITAVAI
                     VALVLWERRVRLPILPVDLLRRGFFAATITANFLVGSALMVGMVNVPVLVALVRNPDT
                     VSRDSALLLAPLTLFIAVFALLSGPISERIGTFPMTAVGVTLTVLGYCVLYLVVDRDN
                     ITKMTIGLALAGMGIGLLLAPLSAVALDASSGENRGAAASTALVFRLLGMTIGMSLLT
                     AAGVRRLQQLTGRLDPVIQEAEESTAEYLARQQQFIDDHVVPLGVQVMQETFLAAGVL
                     VALAIVPVVLMRHAEEGD"
     gene            complement(1710..2435)
                     /locus_tag="VGR29_05325"
     CDS             complement(1710..2435)
                     /locus_tag="VGR29_05325"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEV2073045.1"
                     /translation="MRSNVPRFAAAFLALLLLLTATACGSEESASLDENISAERVLEN
                     ASAQLAETESMHFRMDVEGETLIDPAGTFRLLNAEGDMARPDRVQVEFQVEVLGSKTI
                     SIRMISIGTESWTTDLVTGDWVPAPSEFGYDPTILYDNQNGLGPVMGKVDNAEIVGIE
                     EIDDHQTYHVRGTSPGEVMKPITSGTMQGDNIGLDLWIDGETWNLIRVVVSEPDAPEV
                     DDPATWTMTLSNHGEQVDIEPPT"
     gene            2687..3598
                     /locus_tag="VGR29_05330"
     CDS             2687..3598
                     /locus_tag="VGR29_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEV2073046.1"
                     /translation="MSAAAHDLQLTVGSMTHTGGRENNEDSILVESLPPMTPGSGPGY
                     LLAVADGMGGYEGGEIASKLAIDLVRDLFARDQPADVALALKQAYRRANDAIYSQGAG
                     TTDAPPMGTTLVTAVVRGKYVTIANVGDSRAYLMRANQITQVTQDHSLVAEQVQQGQI
                     SEDQARKSRQRNVLTQALGTKETLDKRMPSIYELALLPEDRLLLCTDGFFDVLTHDDY
                     LQLMSGNDPNEAAAQLTSLAVERRTTDNVSAIVLAVGASLTTVQRQQISAELAEQRGN
                     AMGAMLIPLLVLIVVIIAIVAGIYFYM"
     gene            complement(3875..4192)
                     /locus_tag="VGR29_05335"
     CDS             complement(3875..4192)
                     /locus_tag="VGR29_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEV2073047.1"
                     /translation="MIITTTPAVEGRPVQRYLGIVSGEAILGANIMKDLFAGVRDIVG
                     GRSKAYEKELGKAREIAMQEMEELAASLGANAIVGVDLDYETVGSQGGMLLVSVSGTA
                     VQI"
     gene            4319..5212
                     /locus_tag="VGR29_05340"
     CDS             4319..5212
                     /locus_tag="VGR29_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2073048.1"
                     /translation="MKTLPSEAPLPPVPPIDAHTHLFPPEIIRRREQLLRRDPWFGQA
                     FGTPKAKMIGEDSLLASMEAAGYAMSVVLGWPWRDQGLCREHNAYLADVARRYPSLIA
                     WLGIVNPCLPDASSEIRFCADRGAAGFGELNADGQGFDWADPNQTAEAFGSFAGAGLP
                     VLIHTSEPVGHSYAGKGSATPDRLLRSLAAFPGVQFIMAHWGGGLPFYELMPEVRELT
                     RNVVYDSAASTYLFDFRIFPVLEQIVGPERLLFATDYPVLAQGRFLQRVLSSGLSDAA
                     MPQVLAGNSRRVFNLKERTGS"
     gene            5209..5790
                     /gene="ruvA"
                     /locus_tag="VGR29_05345"
     CDS             5209..5790
                     /gene="ruvA"
                     /locus_tag="VGR29_05345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV2073049.1"
                     /translation="MIAGLIGSIHRAEQDAILVNVHGVLYRVFTTGRVVSTASEQIGE
                     REFFTHQVVREDAQLLYGFLTPADLQWFQTLISVNGVGPRMALAVLTRFSADELLSVI
                     ANEQIDLLVTVPGVGKRTASRMILDLRGKLPENLETPVSLAGLSNPDALEALQALGYT
                     FAEARSALGHVKLADDATVEDHIVAALRELGGD"
     gene            complement(5787..>6114)
                     /locus_tag="VGR29_05350"
     CDS             complement(5787..>6114)
                     /locus_tag="VGR29_05350"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEV2073050.1"
                     /translation="APEHLCLLVKDPWALVPRVRNAGGVFIGESSPEALGDYTAGPSH
                     VMPTAGTARYSSPVNIGDFQKVISLLAGNEKAINELGPATMRIAEAEGLGGHARAIER
                     RLDRNR"
BASE COUNT         1304 a         1777 c         1822 g         1211 t
ORIGIN      
        1 gcctggaggt tgatggcggg ataaacctcg gcactatagg ggcagtggtc gacgctggcg
       61 cggatatgat cgtcgcagga agcgctgtat tcagcgccgg taaatccccg tcagaaaacc
      121 tcgcggcact ccgcaacgcg attcgttagt cgccctcctc ggcgtggcgc ataagcacaa
      181 cagggacgat ggctagcgcc accaggacgc ccgccgccag aaacgtttcc tgcattacct
      241 gaaccccgag cgggacgaca tggtcgtcga tgaactgttg ctgtcgggca agatattccg
      301 cggtagactc ctcggcttcc tgaatcaccg gatccaggcg acccgtgagc tgctgaagcc
      361 ggcgcactcc cgcagcagtc agcaaagaca taccaatagt cattcccaaa agtcgaaaga
      421 caagggctgt tgacgcggct gctcccctat tctctccact cgatgcatca agggccacgg
      481 cgctcaaggg agccagcagc aaaccgatac ccatgccagc caatgccaac cctatagtca
      541 tttttgtaat gttgtcgcga tctacaacca gatagagtac gcaatacccg agaaccgtca
      601 aggttacacc aacggcggtc atagggaagg tgccgatgcg ctcggagatc gggccagaga
      661 gcagcgcaaa aacggcaatg aagagcgtca acggagcgag tagaagggcg ctatcccggc
      721 tcacagtatc gggattccga acgagggcca cgaggacagg gacgttgacc attcctacca
      781 tgagcgctga gcccaccagg aaattcgcgg taatcgttgc ggcgaagaag ccccgtcgga
      841 gcaggtcaac ggggagaatc ggtagcctga cgcgccgctc ccagagcacg agcgcgacaa
      901 tcgccacagc ggtgatacct agcagcaagg ggatgtgatc agcaaatgga ttgggcgtgc
      961 cgccaagcgc acggagccca gatcccgcct ttgcgccgaa ctcgcccccg gaagccaacg
     1021 ccaaattaac gcagatcagg gcaatcgcga gaagcaaagc gccggggaca tcgacagccc
     1081 tggcggcttc acgtttccgc agatgcgaac ggtgtggaaa gccctttaaa atagcaacca
     1141 aggcaacgag caggaaaggg aggttgagcc agaagaccca tcgccatcgt tcgtctgtgc
     1201 cgggcaaaag gccaacgatg actgcgcccc acgtcggccc aaggacccag ccgagtgtgt
     1261 caacggcgct caccaggccg atcccggcca gcctcatccg ccgaggaagg acatcgctcg
     1321 cgagcgctat ggtgacgggt aagagtcctc caccgccgag accctgaatc gtccgcccgg
     1381 cgacaagctc cgagagggac tcggcagtcg cgcagagaat cgagccaaca gcgaagatgg
     1441 ccaggcaggc aaaaaacgca gcttggcgtc cgaagacatc cgagagcctg ccgacgagcg
     1501 ggattgcgac gatgtaagca atgagatacc cgttgacaac ccaaatatag cggtcgatgt
     1561 ctgccgtatt gacgccgagg tctggaataa tggtcgaaag aatcgaggct ataacggtca
     1621 gatcgattgc gcccaggaag acgcagatca accccgctgt cagtgcccgc gaggaaagga
     1681 tgttcctgaa atcgatccgc tctgcggcgt catgtcggag gttcaatgtc cacctgctcg
     1741 ccgtggttcg aaagcgtcat tgtccaggtc gcgggatcgt caacttcggg cgcatcaggc
     1801 tccgaaacga caacccgtat cagattccag gtttcgccat cgatccaaag gtcgagacca
     1861 atgttgtcgc cttgcatcgt gccgctggtg atcggcttca tcacctcacc tgggcttgtt
     1921 ccacgaacat ggtacgtttg atggtcgtca atctcctcga ttccaacaat ctcggcattg
     1981 tccacctttc ccattaccgg cccaagaccg ttttgattgt cgtagaggat cgtcgggtca
     2041 tatccgaact cgctcggcgc agggacccaa tcgcccgtaa cgagatcggt ggtccaggac
     2101 tctgttccaa ttgagatcat gcggatggag atggtcttcg aaccgagcac ctcgacctga
     2161 aattccacct gcacccggtc cggacgggcc atatcgccct cggcgttcag caatcgaaac
     2221 gtgcccgcgg ggtcgatcag ggtttctccc tcgacgtcca tccggaaatg catcgattca
     2281 gtttcagcga gttgggcact cgcgttctca agcactcgtt cggcggagat gttctcgtcg
     2341 agtgatgcgg attcctccga cccacacgcg gtcgcggtga ggagcagcaa gagcgccagg
     2401 aaagcggcgg caaatctggg aacgttcgag cgcaatggag atcctgacgc taatccgggc
     2461 acaagaagaa caaacaggaa aatggcttcc tgcctcaagc cggcaaaccg gggtcatcca
     2521 gagtttatca tctgctttcg gtatgcttgt caccgaacgc tgatggtact aatcaggttc
     2581 ggcaagcgga gccattcgtt ctttcaacac gatcatcact ctaattggac ggcgaagtgc
     2641 ggaccctccc gtccgtgatc gccttgaaac aatcgggagc cccattttga gcgctgcagc
     2701 acacgacctc cagcttacgg ttggcagcat gacgcacacc ggagggcggg agaacaacga
     2761 ggattcaatc ctcgtcgagt cgttaccgcc aatgactcct ggatccggtc cgggttatct
     2821 tctcgcggtc gcggacggca tggggggcta cgagggaggt gagattgcca gcaagttggc
     2881 gattgatctg gtccgtgatc tctttgcccg agaccaacct gcggacgttg cactcgcgtt
     2941 gaagcaggcc tatcgccggg cgaacgatgc gatctactcg cagggagcgg gaactaccga
     3001 cgctccgccg atgggcacga ccctcgtaac ggcagttgta cgaggcaaat acgtgacgat
     3061 tgccaatgtc ggtgacagtc gcgcgtacct catgcgagcg aatcagatca cccaggtgac
     3121 gcaggaccat tcgctggtcg ctgagcaagt gcagcaagga cagatctccg aggaccaggc
     3181 ccgcaagagc aggcaacgga acgtcttgac ccaggcgctc gggacgaagg agacgctcga
     3241 caagcggatg ccaagtatct atgagctggc tcttctgcca gaggatcggc tgctgctctg
     3301 caccgatggt ttttttgatg tccttaccca tgatgactat ttgcagctta tgtcaggaaa
     3361 tgacccgaac gaggcggcgg cgcagctgac gtcgctcgcc gtggagagga gaaccaccga
     3421 caacgtgagt gcgattgtcc tcgcggtagg ggcatcgctt acaaccgtgc agcgtcagca
     3481 gataagcgcg gagttggcag aacagcgcgg aaatgccatg ggagcgatgc tcatcccgct
     3541 actcgtgttg attgtcgtga tcatcgccat cgtggcgggc atttatttct atatgtagcg
     3601 cgaacgggaa gactagagcg cgaacgtcac tactaaaaat caatcccctc accccgtgtg
     3661 tgagggaatc atgatcctcg cacacaggca ttacagtgct tgattcactc tggtcgtcgc
     3721 tcgcggacgc catgatgctg tgcaaacctc cgcgcattca atggcggatt tgtgggtagc
     3781 tcgatatcgg agtgttgcgt gatacgcgag accgtcgttc ccgcccttgc cgggatgatt
     3841 gcccttcaca atcggcaacg ggcgtgcagg acatctagat ctgtacggcg gttcccgaaa
     3901 cagaaaccaa gagcatgcct ccttgagatc cgacagtctc gtagtcaagg tccaccccga
     3961 cgatggcgtt ggcaccaagg gaagctgcca gctcttccat ttcctgcatg gcgatttccc
     4021 gtgccttgcc cagttccttt tcatatgcct tggaccggcc gccgacgatg tcgcggaccc
     4081 cggcgaagag gtccttcatg atgtttgcgc cgaggattgc ttctccagaa acgatcccca
     4141 ggtatcgctg cacggggcga ccctcgacag caggtgtggt ggtgataatc atcattgagc
     4201 tccgtttatc gcatccgttt gattgggacc tgaacaccgc atacggtcgg tgaaggcggg
     4261 gacggaaatt acctggcgcg accagaatag acaacgtccg tcgggtacca ttcgttccat
     4321 gaagaccctg ccgtccgagg cacccttgcc tcctgtcccg cccatcgacg cgcatacaca
     4381 tctctttcca cctgaaatca tccgccgccg ggaacaattg cttcgccgcg atccatggtt
     4441 cgggcaggcg ttcgggaccc ccaaggcaaa gatgatcggt gaggatagtc tgcttgcaag
     4501 catggaagcg gccggctacg caatgtcggt ggtgcttggc tggccctggc gtgatcaggg
     4561 actctgtcgg gagcacaacg cgtatcttgc cgacgtggcg cggcgctatc cgtcccttat
     4621 cgcctggctc ggtatcgtca atccatgtct gccggacgca tcctccgaga tccggttctg
     4681 tgctgatcgc ggtgctgcgg gattcggcga gttgaacgcg gatggacagg gatttgactg
     4741 ggcagatccg aatcaaactg ccgaggcttt cggcagcttt gcaggagcgg gtctgccggt
     4801 gctcatccac acgtcggagc cggtaggcca cagctatgcc ggcaaggggt ccgctactcc
     4861 cgatcggttg ctgagatcgc ttgccgcctt tccaggcgta cagttcatca tggcccactg
     4921 gggcggcggc ctcccattct atgaactgat gcctgaggtt cgggagctga cacgtaatgt
     4981 ggtgtacgac agtgctgcat caacgtatct gtttgacttc aggatctttc cggtgctaga
     5041 gcagatcgtt ggtcctgagc ggttgctctt tgcaaccgac taccccgtac tggcccaggg
     5101 gcgattcctg cagcgggtgt tgtcttccgg gttgagcgat gctgcaatgc cgcaagtgct
     5161 tgccgggaac tcacgccgag tgttcaatct caaggagcgg acaggttcat gatcgccgga
     5221 ctgattgggt cgattcatcg agcggagcag gacgccattc ttgtgaacgt tcacggagtt
     5281 ctttaccgcg tcttcacgac agggagagtc gtttcgaccg catccgagca aataggggaa
     5341 cgggagtttt tcacccacca ggtcgtccgt gaagacgcgc agctcctgta cgggtttctt
     5401 acaccggcag acttgcagtg gttccagacg ctgatatcgg tcaacggggt ggggccgcgt
     5461 atggcgctgg ccgtcctgac gcggttctcg gcggacgaac tcctgtcagt tatcgcgaac
     5521 gagcagatcg acctgctggt aacggtgccc ggtgtaggca agcgtacagc ctcacggatg
     5581 attctcgatt tacgcggaaa gctgccggag aacctggaga cgcctgtctc cctggctggg
     5641 ctcagcaatc cggacgcact tgaagcgctg caagcgctgg gatacacctt cgcggaagct
     5701 cgcagtgcgc tcgggcatgt gaagttggct gacgatgcca cagtcgagga tcatatcgtt
     5761 gccgcgctgc gggagcttgg cggtgattag cggttgcggt ccagacggcg ctcgatcgcc
     5821 ctcgcatggc cgcctaaacc ctctgcctcc gcgatcctca ttgttgccgg ccccaattca
     5881 ttgatggcct tctcgttccc tgccagtagc gagatgactt tttggaagtc tccgatgttg
     5941 accggcgagg agtatcgtgc ggttcccgcc gtgggcatca cgtggctggg gccggcggtg
     6001 tagtcgccaa gtgcttcggg gctggactcg ccaatgaaga cacctcccgc atttctgacc
     6061 cggggaacca atgcccaggg gtcctttacg agcagacaga gatgctccgg cgcg
//
LOCUS       DASYWM010000131         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_342440, whole genome shotgun sequence.
ACCESSION   DASYWM010000131 DASYWM010000000
VERSION     DASYWM010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342440"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="VGR29_05355"
     CDS             <1..1435
                     /locus_tag="VGR29_05355"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEV2073051.1"
                     /translation="ALYDQLKNMIGGDDESTPPTVENVDYNGTTIETITGTGDEATPA
                     SLALVGETVVLAANPADIEPIVDTVNGDLEPLSSNQQFAEVRRSLEGPSLSFGYANGA
                     VIVEQLATEEPAAADFTEGMAGYFGWNAYVDDSGFRLDTVAIPAEGAEPGMVAAFDPT
                     FAEKVPADTLYFLNGNNLAESGIFDLFGFALQASLAGTGTDISATPVATPTVDDVYAQ
                     LESQLGFNLKTDLFDQLDGEFALAVNVDQIFSEEPVTDAVFVSDVADEMSVADVASKI
                     SFILNSSLEEGATLSEREVTGGTVTSITIDESSTGGFSVVLEYGVVDGQLLIGVNNGI
                     DNYLDGTTDTLARDPNFQQTMSALPQENIVGVQYLSLAKLLPMIEEAAMGVSASTSVL
                     DNDMACGEYATQEEAQAAYDADSIGLWNLDLDYDGMACEDFFTVAASPVASPASVSEN
                     LNLVSLGTVSYTEEDTYKTSTLLLVGE"
     gene            1537..2598
                     /locus_tag="VGR29_05360"
     CDS             1537..2598
                     /locus_tag="VGR29_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV2073052.1"
                     /translation="MNETLLRDLTQTPGIPSREDRIRDVTAAHMRPLVDEMYVDVLGN
                     LIGTRKGKGGPRVGIAAHVDEIGFLVRHVDDKGFIRVQPVGGFDPRVLVAQRAEIHCR
                     DGRSLLGVIHPGTKPVHLLQPGESKETKLEDLFIDLGLDAAAVREQVNIGDMVTMRRD
                     LEMMGDVVVSKALDDRLGVYVMLEAIRAAGETNAEIIAVATVQEEVGLRGATTSAHGV
                     KADIAIALDVTIAADTPGIPADSAVTEMRGGTAIKVFDSSQLPNPKLVDHLREIAVTN
                     SIPFQMEILPKGGTDAGAFQRTRDGVVTGTISIPCRYVHSVNEMAAVSDIQASVDLLA
                     QFLQVAGTRSYGYDLPDEG"
     gene            2698..>4129
                     /locus_tag="VGR29_05365"
     CDS             2698..>4129
                     /locus_tag="VGR29_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2073053.1"
                     /translation="MTSRTRSIRQRSSFSQLVTAGVVPHSRRDFMRASAILAGAAAAG
                     FRPVSAREFPALPFRGLIQDDVQTGVEIAIPFNPYGQDVTLDPHRAPNWGPFWVMLPY
                     AWSGLLRFNEDGGVEPDLAESVTPNSDGSVWTAVLKEGISYGNGDPVLADHFVGSWKR
                     ALDPLQLSPMAQFMEAVEGFSEIEAGGEAELGFSVIDDRTIEITLTRPVSHFPSSLAT
                     FVWAVMHPAYMDQVSESAFPLQDAGAGPWKITEFDEGNRFVMTPNEHYWGSPSPSVTS
                     IAWHILPGGGADQAALDLYAQNLAVVADVPHWLLPGVRDDGTLSTELVEIEDHASTLA
                     VSMDFHQPPFNDVRVRRAIASAIDTGRWATDIVGGAYVSAMSFTPPVLRTIADYTAPE
                     GLTSGAEGPATLPDDADLKSEIFYFQPATDTPEQMESASLLLGMIEEGTGIAITHDTS
                     LTPEQITALRQDNGGLQFNIVQWWLAS"
BASE COUNT          852 a         1231 c         1211 g          835 t
ORIGIN      
        1 ggccctgtac gaccagctca agaacatgat cggtggagat gacgagagta cccctccgac
       61 ggtagagaat gtggattaca acggcacaac gattgagacc atcacgggga cgggtgacga
      121 ggccacaccg gcatcgctcg ccctcgtcgg tgaaacagtc gtgctggcag cgaacccggc
      181 agacatcgaa ccgattgtcg acaccgtcaa tggtgacttg gagccacttt cctccaacca
      241 gcagtttgca gaggttcgac gctctctcga gggtccatct ctcagctttg gctatgcgaa
      301 cggtgcagta attgtggagc agctggcaac agaagagccg gctgcggcgg acttcactga
      361 gggcatggcg ggctactttg gatggaacgc ctacgtcgac gacagcggct tccgcctgga
      421 taccgtcgcg attcccgctg agggtgctga gccaggcatg gtagctgcct tcgatccgac
      481 gtttgcagag aaggttcctg ctgatacgct ttacttcctg aacggcaaca acttggccga
      541 atcggggatc tttgacctct tcggattcgc ccttcaggca tctcttgccg ggacgggcac
      601 ggatatctca gctactcccg ttgcgacgcc aacggtcgac gatgtctatg cccagctcga
      661 atcccagctt ggcttcaacc tcaagacaga tctctttgac cagctcgatg gtgagttcgc
      721 cctcgccgtg aatgttgacc agatcttctc cgaagagccg gtgaccgacg ccgttttcgt
      781 gagcgatgtg gcggacgaaa tgtccgtcgc cgacgttgcc tccaagatct ccttcattct
      841 gaactcctct cttgaggagg gcgcgacact gagtgagcgg gaggttacgg gcggcactgt
      901 tacgtcgatt accatcgacg agtcaagcac aggtggattc tcggtggtgc tagagtatgg
      961 cgttgtcgac ggccagctgc tgatcggcgt caataacgga atcgacaact accttgatgg
     1021 cacgacagat accctcgccc gggatcccaa cttccagcag acgatgagcg cgctcccgca
     1081 ggagaacatt gtcggcgttc agtatctgag ccttgccaag cttctcccaa tgatcgagga
     1141 agcggcaatg ggtgtctccg cctcgacctc ggtgctggac aacgacatgg cgtgcggcga
     1201 atatgccaca caggaggagg cccaggccgc ctatgatgca gattcgatag gcctctggaa
     1261 cctcgatctg gactatgacg gcatggcgtg tgaggacttc tttactgtgg ctgcgagccc
     1321 ggtggcttcg cctgcaagcg tctccgagaa tctcaacctt gtctcactcg gcacggtatc
     1381 ctataccgaa gaggatacct acaagacaag cactctcctc ctcgttggag agtaaccgac
     1441 ccgagatacc ggcttcggtg taacctgaag ggcgatgtgg cgaacgtcac atcgcccttc
     1501 tcgttgtcca cggatacaca tggagtgatg ctctcgatga atgagaccct gttgcgcgac
     1561 ctgacccaaa ctcctggcat tccatcccgc gaggaccgca tccgcgatgt gaccgccgcc
     1621 catatgagac ccctggtgga tgaaatgtat gtcgacgtcc tcggcaacct gatcgggacg
     1681 agaaaaggca aaggtggtcc ccgtgtcggt attgccgcgc acgtggacga gattgggttc
     1741 ctcgtacgcc atgtggatga caagggcttc attcgcgttc agcctgtcgg aggattcgac
     1801 ccacgcgtgc tcgttgccca acgggccgag atccactgcc gcgacggtag gtcacttctc
     1861 ggcgtcattc accccggcac caagccggta catctcctcc aaccaggaga gtcaaaagag
     1921 acgaagcttg aggatctctt cattgacctt gggctggatg cagccgcggt acgggaacag
     1981 gtcaacattg gggacatggt aaccatgcgc cgcgacctcg agatgatggg ggacgttgtc
     2041 gtcagtaaag ccctcgatga ccgcctcggt gtctacgtga tgcttgaagc aatcagggcc
     2101 gccggagaga caaacgcaga gatcattgcg gttgcaactg ttcaggagga agttggactg
     2161 cgaggtgcga caacgtccgc ccatggagtc aaggccgata tagcgatcgc tctcgatgtc
     2221 acgatcgcgg ccgacacgcc cggtatcccg gccgactccg cggtaacgga gatgcgaggc
     2281 ggcacggcaa tcaaggtctt cgacagctcc cagttgccga atcctaaact ggtcgaccac
     2341 ctgcgcgaga tcgcggtaac gaacagtatc ccctttcaga tggagatttt gcccaagggt
     2401 ggaacagacg ccggcgcctt tcagcgaacc cgggacggtg tggtcacagg aaccatctcc
     2461 ataccttgca ggtacgtcca cagcgtgaac gagatggcgg ccgtctcgga catccaggca
     2521 agcgtcgacc tcctcgcgca attcctccag gtcgctggta cgcggtcata cggatacgac
     2581 ctcccagatg aagggtaacc atttcacggt tggacgcgtt tgatatgtga gtagtcctcc
     2641 cgacacacgc gtcatcggtg gaatcaaacg cctgcatcat tcacgaggag ctgtgaggtg
     2701 acatcacgaa ctcgttcaat ccgccaaaga tccagctttt cgcaactggt cacggctggc
     2761 gtcgtacctc attctcgccg cgacttcatg cgcgcgtccg caatcctggc cggggcggct
     2821 gccgccggtt tccgtcccgt ctctgcccgc gaattccccg ccttaccgtt ccggggactc
     2881 atccaggatg acgtccaaac cggagtcgag atcgccattc ccttcaaccc ttacggccag
     2941 gacgtgacac tcgaccctca tcgcgctccc aactggggtc ccttctgggt gatgctgccc
     3001 tatgcatggt cgggactcct tcgcttcaac gaggacggag gcgtggaacc tgacctggct
     3061 gagagtgtca cgcccaatag tgatggatct gtctggacgg cagtcctgaa ggagggaatt
     3121 tcctatggca atggtgatcc tgtcctggca gatcacttcg tcggatcgtg gaaacgcgcc
     3181 ctggatcccc tgcagctctc tcccatggcc cagttcatgg aggcggtaga aggattctct
     3241 gagattgagg caggcgggga agccgagctg ggtttctccg tcatcgacga tcgaacaata
     3301 gagatcaccc tcactcgtcc ggtgagccat ttcccctcct ccctcgcaac gttcgtttgg
     3361 gcagtaatgc acccggcgta catggatcag gtaagcgagt cggcatttcc gctgcaggat
     3421 gcgggagcgg gcccatggaa gatcactgag tttgacgaag gcaatcgctt tgtcatgacc
     3481 cccaacgagc actattgggg tagcccttcc ccatcggtga ccagcatcgc ctggcacata
     3541 ctgcccggtg gtggcgccga ccaggcagcg ctggatcttt acgcgcaaaa cttggccgtg
     3601 gtcgccgatg tgccgcactg gttgctgccc ggtgtacgcg acgacgggac gctatcgact
     3661 gagctggtag agattgagga ccatgcgtcg acgcttgcag tgagcatgga cttccaccag
     3721 ccgcccttca atgacgtgcg cgttcgccgc gccatagcct cggctattga taccggtcgc
     3781 tgggccacag acatcgtggg gggggcatac gtttcagcca tgagcttcac gccacctgtt
     3841 ctcagaacga tcgcggacta caccgctccg gagggtctga cctcgggtgc tgaaggtcca
     3901 gcgactctgc cggacgacgc cgatctgaaa agtgagatct tctacttcca gccggccacg
     3961 gacacgcctg agcagatgga aagcgccagc ctgctcctgg gaatgatcga ggagggcact
     4021 ggcatcgcca tcacccatga cacgagcctg acgccggagc agatcacggc cctgcgccag
     4081 gacaacggcg gactgcagtt caacatcgtg caatggtggc tggcatcga
//
LOCUS       DASYWM010000132        18222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_380324, whole genome shotgun sequence.
ACCESSION   DASYWM010000132 DASYWM010000000
VERSION     DASYWM010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 18222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18222
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380324"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VGR29_05370"
     CDS             <1..1373
                     /locus_tag="VGR29_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073054.1"
                     /translation="PHAFYWFDLSRPPASNAVRLGARNAVAEGQALPIITTQGAWGSV
                     VADQDRYQLVQQLPDFLGDQPWFRGRSDSILNVSIIDAIPLRPGEASTWLVPISVAYS
                     DRDVESYLLALQSLPVDADGKTGHELRLPIARLHSAVDGEAVLVDAIEEGITEASLAW
                     VTDKRSEPLPGSLGELVVLRTDEGETAAPSRVISHVEEQSGNIRKGEERVLVKVLRHI
                     GDGTHPEWEIGSALTNNGFPHVPAVLGALEYRRAQQPPMTLAVLQALVPHEGNAWQFT
                     LGVVDEFLSEAMSQGVVLPQDFRPTAMALLDATDHLPPQPLASLLAAYLPFARLLGQR
                     TAELHSALASIQDRAAFAPEPQTTFSQRSLYQSMRGLTSGVLRALRHVMVNLDGEERE
                     LAVNILNSEGALYDRFRTVLMQPLTGAKIRTHGDLHLGRSFGSMMILCSLTLKANRIA
                     IWRSVA"
     gene            1319..1723
                     /locus_tag="VGR29_05375"
     CDS             1319..1723
                     /locus_tag="VGR29_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073055.1"
                     /translation="MFVDFEGEPDRYLEERRLKGSPLRDLASMLHSFRDAAMTEAERR
                     SGTVAMASEERATIEALALAWYRWGAATFLAGYLDTAGDAAYLPASREEWATLLDAMM
                     LEKTIYALGYALNHAPNQLGRPLRNVLQLLWG"
     gene            1983..2438
                     /locus_tag="VGR29_05380"
     CDS             1983..2438
                     /locus_tag="VGR29_05380"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV2073056.1"
                     /translation="MSTPEERLQELGIELADFTPFHPAVAAGVITGGNLLFVSGSTPS
                     SPDLRGRVGDTLTIEQGYQAARECAIGQLAAAKSVLGDLSRIRRVVKLLGMVNAAPGF
                     GETPAVIHGASEFYYEVFGEKGVHARSAVGLQSLPNDVAVEIETIFEIE"
     gene            complement(2588..3466)
                     /locus_tag="VGR29_05385"
     CDS             complement(2588..3466)
                     /locus_tag="VGR29_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV2073057.1"
                     /translation="MFAVGKRAIKDFIHDDMNTYAGALTFRILLAIFPFLIFMLTLLD
                     VLGLTNFFNWLLDQAQKAFPSDLYSQFEMIVQQVRGGANGGLLSFGLILALWAASGGV
                     RAAMNALNAAYDVEESRPIWKKYPLSIVYTVGLAALLIAAVALMLLGPQTMEWLAGQI
                     GLGDVFVTIWAWLRIPAAILIMMLAVALVYYFFPNVDQPFQIVSPGSVVAVLTWVVAT
                     IGFSFYISNFANYNATYGSLAGVVVLLLYFFISSAVLLFGAEINGVIYKAEHGTEDDN
                     VEDSAVQEARQETTTA"
     gene            complement(4275..4991)
                     /locus_tag="VGR29_05390"
     CDS             complement(4275..4991)
                     /locus_tag="VGR29_05390"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEV2073058.1"
                     /translation="MHHNVPAVTGAAMARIDRIMIQEMGVDMLQLMEIAGFAVADVAR
                     RHCGLDLTQQPAIVALAGTGGNGGDAMVAARLLHAWGARATVVLLKPRQEFSGIAAHQ
                     LAILDRMGIPVQEPGDNDPPPAEVIIDGLFGFSLQGNPRDEAARLITLANAQPAPVLA
                     IDVPSGLDATSGEVSDPCIRARWTVTLALPKTGLIVAPADITGAIWLADIGVPPDIYA
                     RIGVDVPGDLFANRGIIPIL"
     gene            5244..5852
                     /locus_tag="VGR29_05395"
     CDS             5244..5852
                     /locus_tag="VGR29_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2073059.1"
                     /translation="MVDAQKVKRRTADERREEVIQAAIVEFATYGFHGGSTERIADAA
                     GISQPYVLRLFGTKKALFLAALDQVSTDIIDAWSQALARHPGGTSDERLDVLRETYRV
                     FVEDVVQLRLVLQGFSSSEDREVRVQSQECLGRMFAWTREATGADAEQVRLLFAQGMT
                     LMVAASIRAWERAGSEEWARAMLMMPLDEVPTLRQGVRVNHS"
     gene            5916..6356
                     /locus_tag="VGR29_05400"
     CDS             5916..6356
                     /locus_tag="VGR29_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073060.1"
                     /translation="MKEADTHSPSNPQQILGAVSWVLLIAVMFGGESLLLMLSSGEGM
                     SAHREQFFRAGHGHAGVLAAIGILYSNYIGRTFLLVRAQVIAWVAYATGVSMIAGGMF
                     LHAYVGTAGSGSPGTFLTAVGGVVLAGAVLFLAWQLIRARGGVA"
     gene            6353..6727
                     /locus_tag="VGR29_05405"
     CDS             6353..6727
                     /locus_tag="VGR29_05405"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HEV2073061.1"
                     /translation="MTNDQRGADRRFRADMPEGTVVFLIGMRINRFPALRQRLPVFQA
                     MPKMLAELKRHPELGLLDASSWWAGRNLMTVQYWASMDQLMRYATAKDAEHFPDWQQF
                     MRRSTDADAVGIWHEVYEVQPQ"
     gene            7171..7830
                     /locus_tag="VGR29_05410"
     CDS             7171..7830
                     /locus_tag="VGR29_05410"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV2073062.1"
                     /translation="MQHPSESVLDSGSSDSLLADYQRQMTICRRCVTAGYLKEANPVF
                     HGFATQRVMIIGQAPGVRAHATGVPWSGRSGEILRGWLEIAGFPPEQWRETWYLTSLT
                     KCFPGKATQGKGDRAPSGAEIALCADHLQMELQLVRPEVIVTLGKMAASRVIPGANRQ
                     SLATLVGSLAQVDLPHGSMIVVPLPHPSGVSRWLNDLANRARVDEGLALLARERERRG
                     L"
     gene            complement(7930..8718)
                     /locus_tag="VGR29_05415"
     CDS             complement(7930..8718)
                     /locus_tag="VGR29_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073063.1"
                     /translation="MKTTRFNAITRWVWPMLLLIALGLGWEIAVRLTETPRWMLPPPS
                     AVFTSFYEDWSLLMQHTRVTLVEVLIGFVLALVAGIATGVAIDSSRIIERAVYPLLIA
                     SQTIPMVVLAPLFLIWFGYGLLPKVLITALVAYFPLAVNTVDGLRSGDREILALLRSM
                     GASRWQRFRLAKVPAALPSIFSGARIAVAFCVIGAVFGELVGAKEGLGYLMERAASQF
                     ETARVFAAIFILAFMGVGLFVAVTLLEHLLLPWRRYITDGSRGN"
     gene            complement(9008..9811)
                     /locus_tag="VGR29_05420"
     CDS             complement(9008..9811)
                     /locus_tag="VGR29_05420"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2073064.1"
                     /translation="MDAPQLGTPLLVARSITAHYEEDGQILQALDDINLTLDDGEFVA
                     LIGPSGCGKSTLLDIISGLYPQSGGAIIIDGEVLTLSQRLGHAAYMRQRDLLMPWRTA
                     LDNACLALEAHGLPKKQARQIARERFPEFGLTGFEDAYPAQLSGGMRQRIAFLRTVLT
                     ERRLLLLDEPFGALDALNRVVLQTWLLSLWERERQGVVLVTHDVDEAIFLADRVVVLS
                     SRPGHIMHVEDIDLPRPRTRQMVTSEAFLRHRAVLLEGLGLLEMEEVPV"
     regulatory      complement(9806..9920)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10288..11304
                     /locus_tag="VGR29_05425"
     CDS             10288..11304
                     /locus_tag="VGR29_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2073065.1"
                     /translation="MSVSQRFDGEHAIRAEGLKKTFGKTEALAGVDLSVPTGTVMGLL
                     GPNGAGKTTTVRILTTLLQPDAGSAEVAGIDVLRHPERVRASIGLTGQYAAVDEILTG
                     RENLVMFGQLYRLSRKAAFARADELLERFKLTDAANRSLKTYSGGMRRRLDLAASLIV
                     APPVLFLDEPTTGLDPRSRNDMWSVIEDLVADGTTVMLTTQYLEEADRLADEIVVVDQ
                     GRIIAQGTADELKVQLGGERLAISVPVGTDLNLAGRALLSAVHTNDLSESDLVVDTDA
                     HQMTVPFPAGSRGLAEAVRKLDAAGLEITDFSLRRPTLDDVFLTLTGHVAEDGEDAAK
                     ENAA"
     gene            11301..12167
                     /locus_tag="VGR29_05430"
     CDS             11301..12167
                     /locus_tag="VGR29_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073066.1"
                     /translation="MSEQTLTLPAAPMAQPRSTTVDLKPVSRGKLGDVLIDSLVLAHR
                     SLLRVPRQLDWLIGVTVMPLMFLLMFRYIFGGTVQATMPAGENAVNYLVVGIVLQGIV
                     FGGMNTALGLATDAKEGLMDRFRSLPMSRVSVLLGRVLADMAIAVFVTTITLLVGLLV
                     GFRPTGDFMNWLAAIGMLLLVAFAMSWVGAAVGLWLKTIEAVNSIGFSLIMPLTFVSS
                     AFTRPEFMPSWLQGFAKNQPFTLAVDTTRGLLTGYPEVGNKGWIAVAWLVGTLVVMIP
                     LSVWLFERRAKS"
     gene            12280..12756
                     /locus_tag="VGR29_05435"
     CDS             12280..12756
                     /locus_tag="VGR29_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV2073067.1"
                     /translation="MSSKDRRPEGAQAAEICSSIIWMGHLIERFANTRLPEANIAPAM
                     SLPRANLLFAVHSAQRNGGSTRMVDIAFDLGVTARTITTMVDALEQQGLIVRVPDAKD
                     RRAIQLEMTDAGRSLMEPLAQAVESASEVIMSPLSRSERETLLHLMTRLIERDMGD"
     gene            12852..14309
                     /locus_tag="VGR29_05440"
     CDS             12852..14309
                     /locus_tag="VGR29_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2073068.1"
                     /translation="MPDPTSSAPESATQVMLSLLDQLRPDQRVVVATLTEAFASAGAE
                     LYLVGGPVRDLLLERPVPNDLDFATSILPERTKEICARISHAGVYDVGEKFGTIGIVM
                     PAGVATIPVEITTYRSEQYEAGSRQPEVAFGSRIEDDLARRDVTVNAIAIDTGSGQVV
                     DPFYGQADLAMGLLRAVGDPDERFAEDPLRLLRVARFVSQLGFAVESGTKAAMTRQAK
                     TLETISRERILAELTKLLCGDYAGHGLDVLLETGLFIVAMPELLPLEGAASNHPGVHR
                     EKDLWEHTKRVIMQAPARPTVRWAALLHDAAKPLTRSVDSRGEVHFFGHERVGADLAS
                     KLLRRLKADKRTQQDVRRLVELHLRPASYDTETWTDSAVRRLALEAGDVLHDLLDLAA
                     ADVTSGRAHKQRAAARRVQGLKDHLARLEEQQALDELQSPLDGNELMVMFDRPPGRWI
                     AEVKDHLRELVIDGELASDEKERAAGIARELLAGN"
     gene            14541..15194
                     /locus_tag="VGR29_05445"
     CDS             14541..15194
                     /locus_tag="VGR29_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2073069.1"
                     /translation="MQPAPDETLLDIATGGGHVTRVFAPHVASVVATDLTPPILEHAA
                     KAFAGWGIGNVTTAVADAEALPFDDASFDIVTCRIAPHHFPDPAAFVREVARVLRLGG
                     RFVLVDSTVPEGEDGALFNRFEKLRDPSHVRSLTAGEWTSLLGDAGFSIEAIEPFMKR
                     HDFADWTSRSRTSAEDREELTRMILGAPGVFGAETAPDDPGTVLAFTDTKTLFLGTR"
     gene            15669..16163
                     /locus_tag="VGR29_05450"
     CDS             15669..16163
                     /locus_tag="VGR29_05450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2073070.1"
                     /translation="MVTIRPIRPDDESFLWDMAWEAAAVSPEMRALGRNATLAVPEIR
                     RYLEAWGRPGDAGVVAVDENNRRLGAAWYRLFSADAKGYGFVAPDIPELSIGVSPAAR
                     GRGVGSALLNALLATAHDEGQRGVSLSVDRQMSARRLYERHGFRDVGISNPDDTSITM
                     MVTL"
     gene            16289..16459
                     /locus_tag="VGR29_05455"
     CDS             16289..16459
                     /locus_tag="VGR29_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073071.1"
                     /translation="MIVLWYNTAVDNGPCPMCGERNDPEIGLERFPVWNGTFILTPLR
                     DKCGQRSALEFK"
     gene            16836..17681
                     /locus_tag="VGR29_05460"
     CDS             16836..17681
                     /locus_tag="VGR29_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2073072.1"
                     /translation="MNHIDPVTNVELQDARIASHQSGMTPGKYTTMNLVDTVTSHNST
                     SMNRRSLLKLMGVGVAALSVGAVSTAERAGAEHTSQLYTVSTAANFRTGPSTSYAIIS
                     VISKGTTFTLNGQTQNGYASITYQSRSGWVLASLVVAAGSSGSQPTPVISGEAWTISA
                     VNLRSGPSTSHQVLRVVPNAAKIGVSTNVQNDFRYVTYQGQTGWMADAYISFTNGGGD
                     DQGGNYKTTTTALNLRAEPSTSAKILTVIPEGARVQVLHSQAGQYVNVNYNSLQGWVA
                     LAYLK"
     gene            17737..>18222
                     /locus_tag="VGR29_05465"
     CDS             17737..>18222
                     /locus_tag="VGR29_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073073.1"
                     /translation="MYAHEYKLELQATAIIDTYHREAIRAEQRRLVQAGRPSHIATAA
                     LGHRDINLDLRSTTQGAITMFSVPVRFLSRGLSRRTTLAAQILTMVVVLVSILSVGGG
                     STAAQGTRVRHTSTTSAIAAGEACFTGLADCSWVDVYIGPGKVAGTTVLCLEINTGAV
                     YE"
BASE COUNT         3553 a         5461 c         5776 g         3432 t
ORIGIN      
        1 ggccgcatgc gttctactgg ttcgacctga gtcgcccacc ggcgtcgaac gccgtgcggc
       61 tcggtgcacg caatgccgtg gccgagggcc aggcgctgcc gatcattacc actcagggcg
      121 cctggggatc cgttgttgcc gaccaggatc gctaccaact cgtgcagcag ctgccggact
      181 tcttgggcga tcagccctgg ttccgtggac gatccgattc catcttgaat gtgtctatta
      241 tcgatgccat ccccttgcgt ccgggggaag catcaacatg gctcgttccg atctctgttg
      301 cttattccga ccgtgacgtg gagagctatc tgcttgccct gcagtccctc ccggtcgacg
      361 cagacggtaa aacagggcat gagctgagat tgccgatcgc acggcttcac tccgccgtgg
      421 atggtgaagc cgtgctggtg gatgcgatcg aggaaggcat caccgaggca tcattggcgt
      481 gggtcaccga caagcgcagc gagcccctgc cgggatcttt gggcgaacta gtcgtgctgc
      541 ggaccgatga gggcgagacg gcggctccct cccgcgtcat atcgcatgtg gaggagcaat
      601 cgggcaacat ccgcaaaggt gaagagcgcg tgctcgtgaa agtcctgcgc cacataggtg
      661 atggcaccca cccggagtgg gagatcggct cggcgttgac gaacaacggt tttcctcatg
      721 tcccggcggt gcttggggcg ctcgagtatc ggcgtgcaca gcaaccgccc atgaccctcg
      781 cagtgctgca ggcccttgtg ccccacgaag ggaacgcttg gcaattcaca cttggcgtcg
      841 ttgacgagtt cctgagcgag gctatgagcc agggcgtagt actaccgcag gatttccggc
      901 cgaccgcgat ggccctgctc gacgcgaccg accaccttcc acctcagccg ctcgcctccc
      961 tgttagccgc ctatcttccc ttcgcccggc ttctgggtca gcggaccgct gagttacatt
     1021 cggcgctcgc gagcatccag gatcgtgctg ccttcgcccc tgagccgcag acgaccttct
     1081 ctcagcgatc gctctaccag tcgatgcgcg gcctgacctc cggcgtgctt cgggcccttc
     1141 gccacgtcat ggtcaacctt gacggagaag agcgtgaact tgctgtcaat atcctgaaca
     1201 gcgaaggggc tctctacgat cgcttccgga cagtgctcat gcagccactc accggtgcaa
     1261 agattcgcac gcacggagat ttgcacctgg gcaggtcctt cgggtcgatg atgatattat
     1321 gttcgttgac tttgaaggcg aaccggatcg ctatctggag gagcgtcgcc tgaagggatc
     1381 gccgctacgc gacctcgcca gcatgctgca ctcattccgg gatgcggcga tgacggaggc
     1441 tgagcgccgc tccggcaccg tagccatggc atcagaagaa cgcgccacaa tcgaggcgtt
     1501 ggcccttgcc tggtaccgct ggggcgccgc aacgttcctc gcaggctatc tggataccgc
     1561 cggggacgcg gcgtacctcc ccgcttcacg ggaggagtgg gccaccctgc ttgatgcaat
     1621 gatgctcgag aagaccatct atgcgcttgg ttacgcgctg aatcacgccc cgaatcagct
     1681 cggccgccca ctccggaacg tgctccagct gctttgggga taggagagcg accaatgact
     1741 ctatcaccct aaaaatcatc agactgatgc agcgcgaatt gctccccggg aactgctggc
     1801 gggatcaggc ctagtgggat agcacccgac tttctggata cgaagatgcg aacggagatc
     1861 tgggagcagc ccgcgtaacg ctacgttcat cgctggtcgc cgccgagcca ctcgcacgcc
     1921 agatgactat cctgcgcgat actgaatacc accgttctct attcagcaat caggaggaac
     1981 gcatgtccac tccagaggag cgtttgcagg aactcgggat cgaattggcg gactttacgc
     2041 ccttccaccc ggcggtcgcg gccggcgtca tcaccggggg caacctgctc tttgtgtcgg
     2101 gatccacccc cagtagcccc gatcttcggg gacgcgtcgg cgatacgctg acgattgagc
     2161 agggatacca ggctgcccgg gagtgtgcca tcggtcaact tgctgcggcg aagtcggtgc
     2221 tgggagatct ctcccgcatc aggcgtgtcg tcaaactcct cgggatggtc aacgccgcac
     2281 ccggtttcgg cgagaccccc gctgtgatcc acggtgcatc ggagttctat tacgaagtgt
     2341 tcggagagaa gggtgtccat gctcgctcag cggtcggact ccagtcgctt ccgaacgacg
     2401 tcgcggtaga gattgagacg atcttcgaga ttgagtaggc acggatgcat atcgttctga
     2461 gaccacgtcg cgacatgatg gacagtcaag ctagggggac aggcagtggc tcgcggcgcg
     2521 ggagagaaaa tcgaatgcca atacctggcc gcgcagtcgg ggcggcgttg accactatcc
     2581 gttcacgtca ggccgtcgta gtttcctgcc tggcttcctg aactgcactg tcctcgacat
     2641 tgtcgtcttc cgtgccgtgt tcggccttgt agatcactcc attgatctcg gcgccgaaca
     2701 atagcaccgc cgacgagatg aagaagtaga ggagcagcac cacgacgcca gcgagcgaac
     2761 cgtaggtcgc gttgtagttc gcgaagttgg agatgtagaa cgagaagccg atggtcgcca
     2821 ccacccacgt cagcacggcc acgaccgacc ctggcgaaac gatctgaaac ggttggtcga
     2881 cgttcgggaa gaagtagtag accagggcaa cggccagcat cattatcaga atggccgccg
     2941 gaatccgtag ccacgcccat atggtaacga agacgtcgcc gagaccgatc tgaccggcga
     3001 gccactccat ggtctgaggg ccgaggagca tcagcgccac cgcggcgatg agcaatgctg
     3061 ccaacccgac ggtatagacg atcgacaacg ggtacttctt ccagatcggt cgtgactcct
     3121 cgacatcata ggcagcattg agggcgttca tcgccgctcg caccccaccg gacgccgccc
     3181 agagcgcgag gatgagaccg aaggagagca ggccgccatt ggcgccgcca cgcacctgct
     3241 ggacgatcat ttcgaattgg gagtacaggt cgctcggaaa ggccttctgc gcctgatcca
     3301 gcaaccagtt gaagaaattg gtcaatccga ggacgtcgag cagtgtcagc ataaagatca
     3361 ggaaggggaa aattgccagc agaatacgaa acgtcaacgc accagcgtat gtattcatgt
     3421 catcgtgaat aaaatccttg atcgcccgtt tcccaacggc gaacacgctc aagttgttga
     3481 atccaggtag tcccatgcgc ctctttccga tccgcgtgtg gtacatgtcg cacctactga
     3541 gcatgcaccc gatcggtatt gccgatgggg aagtgcactc gtcaggttgg acatgccgca
     3601 cggaccgtgc cgatctcatt gtgggccgcc gaagctggcg cctggcgccg tgaatcgctg
     3661 gcaggttccg gtgcagcata aacccgtatt tgtaagtgcg atccagtctg tctggccatt
     3721 tgctctacat ggatacgctc ggggccgatg ctcttgcagc ccaccgtgat gggagacttg
     3781 ccgccggata cgtaatggcc caggtgcacc gtgcacgggc ctccctatcc ggaattttcg
     3841 gaggaggtgt catatcccta atcgattgag aggcctcatt ctggtccctg cacgagcgag
     3901 cacaggctct cctgcgatcg actgtacgta tccaagtaga ccaggcggtc gttttgctgt
     3961 cacgttgtgt gggcccatac ctagtgtggg ttcacgtccg aaggacgtgc ctgtgcatta
     4021 cacaacatcc tgttgccaat tgggaagggt tgttctggag gtacccgcta acccgttcga
     4081 ggtcatgctc accccagaca atatggtcgt gatacccaac ctgccagagg tgacggcctc
     4141 ttgccggttt ctggaacact gcatcgtcat gctcgtatgt agtagacacc aggagtagtt
     4201 gtgaacagtc tgttcacccg gtcatggggc accatatcac gaccttcggt cgtgcacgcg
     4261 ccttgggtgc gaggttacaa gatgggaatg attcccctgt ttgcaaagag gtcacctggc
     4321 acatcgacgc cgatgcgtgc atagatatcc ggtgggacgc cgatgtcagc gagccagatc
     4381 gcaccagtga tatcagcggg agcaactatg aggccggtct ttgggagagc cagggtgacc
     4441 gtccatctgg cgcgaatgca gggatcgctg acttcacccg acgtggcgtc caggccggag
     4501 ggcacatcga tcgccaggac aggcgccggt tgagcgttgg caagggtgat gagacgtgcc
     4561 gcctcatccc tcgggtttcc ctgcaggctg aacccgaaca acccgtcgat gatcacctcg
     4621 gctggcgggg ggtcgttgtc accgggttcc tggaccggga tgcccatccg gtccaggatc
     4681 gccagttgat gcgcggcaat tccggagaat tcttgacgtg gttttaggag gacgactgta
     4741 gcccgggcac cccaggcgtg gagcagccgg gcggcgacca tcgcatctcc tccattgccg
     4801 ccagttccgg ctagggcaac gatcgctggc tgttgggtca ggtccagccc acagtggcgc
     4861 cgcgcaacgt cagcgaccgc gaaaccggcg atttccataa gttggagcat gtcgacaccc
     4921 atctcctgga tcatgattct gtcgattcga gccatcgctg caccggtcac tgccgggacg
     4981 ttgtggtgca tccgcttgtg tctccatatg ttctgcgcca atgcgcagaa aaaagcgtcg
     5041 cagagcaggg cctccgagtt tgggacgtag tgatgcccct gatagaaagc attcgttcga
     5101 ccccgcacac cgagcgtagc agccaccagt tggaggagtg tcgtgcatcc gtaattcgag
     5161 aagcggaagg gtcttgacac cagaacatgt aagtgtacac tcactaacat acggtcaggc
     5221 tatcacagac aggctaagta cgagtggttg atgcacagaa ggtcaaacgg cgtaccgcgg
     5281 acgagcggcg cgaggaggta atccaggctg cgatcgtgga gttcgcgacc tacggctttc
     5341 acggtgggtc gacggagcga atcgcagacg cggcgggtat ttcccaaccc tatgtgctgc
     5401 ggctctttgg caccaagaag gcactcttcc tggccgcgct cgaccaggtc agcactgaca
     5461 tcattgatgc ctggagccag gcgctggcgc gacatcccgg agggacatcg gatgagcgac
     5521 tcgacgtgct ccgggaaacg taccgggtct tcgtcgagga tgttgtgcag ctgaggctcg
     5581 ttcttcaggg gttcagctct tccgaggaca gggaggtccg ggtgcagagc caggagtgcc
     5641 tgggtcgcat gttcgcctgg acgcgagagg cgaccggtgc cgacgccgag caggttcggc
     5701 tcctcttcgc gcagggaatg acgctcatgg tggcggcatc catccgtgct tgggagaggg
     5761 ccggcagtga agagtgggcc cgtgcaatgc tgatgatgcc gctggatgag gtgccgacac
     5821 ttcgtcaggg tgtacgggtc aatcacagtt gatggacaaa aatgtcttgg cggcgcgtgt
     5881 agatagtgat caatcactaa taaaaggatg gcgctatgaa agaggcagac acacattccc
     5941 caagcaatcc ccagcagatt ctgggagcag tctcatgggt tcttctcatt gccgtgatgt
     6001 tcggcgggga gtccttgctg ctgatgctct cgtccggaga gggaatgtca gcccaccggg
     6061 agcagttctt ccgcgcagga catggccatg cgggcgtgct ggcagcgatc ggcattctct
     6121 acagcaacta catcggccgg acttttctcc tggttcgggc ccaggtcatc gcgtgggttg
     6181 cctacgccac cggcgtgtcc atgatcgcag gtggcatgtt tcttcacgcc tatgtcggca
     6241 cggcaggatc aggatcgcct ggaacattcc tgacggcagt tggcggcgtg gtactcgcgg
     6301 gtgcggtgct ctttctcgca tggcagctca tccgcgccag gggaggtgtt gcatgacgaa
     6361 tgaccagagg ggcgccgacc ggcgattccg cgccgacatg ccggagggca cggtggtctt
     6421 cctgatcggc atgcggatca accggtttcc tgcgctgcgt cagcggctcc cggtcttcca
     6481 ggccatgccg aaaatgctgg ccgagctgaa gcggcacccg gagctaggac tgctcgatgc
     6541 gtcgagctgg tgggccggca ggaacctcat gaccgtgcag tactgggcgt caatggacca
     6601 gttgatgcgc tacgcaaccg ccaaggatgc cgagcatttt ccggattggc agcagttcat
     6661 gcggcgatcc acagatgcgg atgctgtggg tatctggcac gaagtgtatg aggttcagcc
     6721 gcaatgatcc catatcgtgt accgcgacat gccagccttt ggcatgggca aggcgacggc
     6781 ctggaagccg gtctcaagcc ttccgcccca gccggtgaca cgccacccgg tgagcactat
     6841 caaggcagcg gcagaacatc cgtaggcccg aagctctcgg aagtgcagct tccctcgcaa
     6901 gtacgttcgt tcctgccggc agcggtagga tgaacgtcac cgggtgcaga tggtagcgac
     6961 gataatgggg cagacgaaac tggctgcacc ggttcagcct cgtcgtaatt gtgttcgtcg
     7021 agatggtgca atagatgcaa cgaccatcag caagcaggtc gatgcaattg cgggggaacg
     7081 tgcggtcgtc agcagatttc gccttgatgg gttggatatg agatgaacgc tgtgatggtg
     7141 tcgtcgaaga gaaaagcaaa tcccgccccg atgcagcatc cgtcggagtc tgtgctggat
     7201 tccggtagca gcgattcgtt gcttgccgac taccagcgcc agatgaccat atgccggcgc
     7261 tgcgtgacgg caggttacct gaaggaagcc aacccggtct ttcatggttt tgcgacccag
     7321 cgggtaatga tcatcgggca ggctccgggc gttcgtgccc acgcgacagg tgtaccgtgg
     7381 agcggcaggt cgggcgagat cctccgtggc tggcttgaga tcgccggttt cccacccgag
     7441 cagtggcggg agacctggta cctgaccagc ctcaccaagt gctttccggg caaggccact
     7501 cagggaaagg gagaccgggc accttccggg gcggagatcg cgctctgcgc tgatcatctt
     7561 cagatggagc ttcaactggt ccggcccgag gtgatcgtca cgctcggcaa gatggccgca
     7621 tcgagggtga tccccggtgc caaccggcag tcgctggcta ccttggtggg aagcctcgcg
     7681 caggtagatc taccgcatgg gagtatgatc gttgtcccgc tgccgcaccc ctcgggtgtc
     7741 tcccgctggc tgaacgatct tgccaaccgg gcccgcgtcg acgaggggct ggcgctcctc
     7801 gccagggagc gggaacggcg agggctgtag gcaacctgct tctgccgatg gccccggcat
     7861 tgagcgaata tcgccaatca agcgcttcgc aggggtcgat gcgccgccct cgccactccc
     7921 tcccatgcat cagtttccgc gcgatccgtc ggtgatgtac cggcgccagg gaagcaggag
     7981 gtgttcgagc agggtgacgg cgacaaaaag cccgacaccc atgaacgcga ggatgaagat
     8041 cgcggcaaag acacgagcgg tctcgaactg cgacgctgcc cgctccatca gatacccgag
     8101 cccctccttg gcgccaacca gttccccgaa cacggcgccg atcacgcaga aggcgacggc
     8161 gatccgtgcc ccggagaaga tcgatggcag ggcagccgga accttggcga gacggaagcg
     8221 ctgccagcgg ctcgcaccca tgctgcggag cagggcgaga atctcccggt caccggatct
     8281 cagtccatcc accgtattga cggcaagtgg gaagtaggcg accagtgcgg tgatgagtac
     8341 tttcggcagc aggccatacc caaaccagat gaggaagagc ggcgcgagca cgaccatcgg
     8401 gatggtctgg ctggctatca gcaacgggta cacggcgcgc tcgatgatcc gcgagctgtc
     8461 gatggcgacg cctgttgcga ttcccgcgac gagtgccagc acgaacccga tcagcacctc
     8521 gacgagggtt acgcgggtgt gttgcatcag caggctccag tcctcgtaaa aggaagtgaa
     8581 gaccgcagac ggcgggggaa gcatccagcg tggtgtctcc gtcaaccgta ccgcgatctc
     8641 ccagcctagc cctagcgcga tgaggagcaa catcggccac acccagcggg tgatggcatt
     8701 gaagcgagtg gttttcatgg cgcggcgctc accatcgaag ggcgagaatg gtattccata
     8761 agggctcgaa acggtagatc aaaacccctg tcattctgag cgcagcgaag aatcctacgc
     8821 gaagcgtgtt ggctgcctgg gagcctcgga tggagcggcg caatcattac cgtgtgccgg
     8881 ctgacgctac cggcttcacc gggtaattcc cacgctgcgc tctggacagg ctcttcgcca
     8941 gtgctcagga taaagagcgc ctccaacaag ggagggaata cgacctctgt acggggcatt
     9001 agtgctctca taccggcacc tcctccatct ccagcaatcc gaggccctcc agtaggaccg
     9061 ctcgatgcct caggaatgcc tcgctggtga ccatctggcg cgttcggggc cggggcagat
     9121 cgatgtcttc gacatgcatg atgtgcccag gccgtgagga gagcacgacc acccgatctg
     9181 cgaggaagat cgcttcgtca acatcatggg tgacgaggac gacaccctga cgttcccgct
     9241 cccacagcga gagcagccag gtctggagga ccacccggtt cagtgcgtcc agggcgccga
     9301 agggctcgtc cagcaacagc aggcgacgct cggtgaggac ggttcgcaga aaggcgatgc
     9361 gctggcgcat accgccggag agctgggccg ggtaggcgtc ctcgaaaccg gtgagaccga
     9421 actctggaaa acgctcccgg gcgatctggc gggcctgctt tttggggagg ccatgcgctt
     9481 ccagcgcgag gcaggcgttg tccagggcgg tgcgccaggg catgagcagg tcgcgctgac
     9541 gcatatacgc cgcatggccg aggcgttggg aaagggtaag aacctctcca tcgataatga
     9601 tggcgccccc tgactgcgga tagagaccgg agatgatgtc cagcagcgtg ctcttgccgc
     9661 acccggaggg gccgatgagg gcgacgaact cgccatcgtc cagcgtcagg ttgatatcgt
     9721 cgagtgcctg gaggatctgc ccgtcttcct cgtaatgagc agtgatactg cgcgcgacga
     9781 gcaggggtgt ccccaattgg ggagcgtcca tggagggttg cctttcctac gccggtatta
     9841 cccggatcag gtcgaacggt cggtgacgga tgcgtggcca ccatctcagc ccggctcccc
     9901 gggcccccgt agcgatcagt tacgcaatcg caagaatgag gaagtgaact gtcccccttc
     9961 tctgccatca tggtggaagt caccggtcaa tgactggaga ccccaaacac gtgactcaat
    10021 tgtaatcaga agtcgttcag gcacgttgag gaaaggagcg acaggcgcac aacggataaa
    10081 tgtccacgct accgggcggg gccggtacgc ttggacgggc cgggtatact ccacggccga
    10141 aggggcccat gcaacccgtc gagaccatgt ggcggtcgaa ccctgtgcct gtggcacgag
    10201 gggagtgatc ctacactctg gatcggcgga atcatcgagc ggtgtcacag gtatgacccg
    10261 tgcatcacac aacggattgg aaccacgatg tcagtaagcc agcgatttga tggcgagcac
    10321 gcgattcgtg ctgaggggct gaaaaagaca tttggcaaga cggaggcgct ggccggggtc
    10381 gatctctcgg tcccgaccgg aacggtcatg gggttgctcg ggccaaatgg tgccggcaag
    10441 acgaccaccg tgcgcatcct gaccacactg ctccagccgg atgccggctc ggcggaggtc
    10501 gcggggatcg acgtgctgag gcatccggag cgggtacgcg cctctatcgg attgacgggg
    10561 cagtatgccg cggtcgacga gatccttacc ggccgcgaga acctggtcat gttcggtcag
    10621 ctctatcgcc tctcccggaa ggccgcgttc gctcgtgcgg atgagctgct ggagcggttc
    10681 aagctcacgg acgcggcgaa tcggtcgctc aagacctatt ccggcggtat gcggcgtcgg
    10741 ctcgatctcg cggcaagcct gatcgtcgcg ccgccggtgc tctttctcga tgagccgacg
    10801 accgggcttg atccccggag ccgcaacgat atgtggtcgg tgatcgagga cctggtggcc
    10861 gacggcacaa ccgtcatgtt gacgacgcag tatcttgagg aagccgatcg cctggcggac
    10921 gagatcgtgg tcgtcgacca ggggcgcatc atcgcccagg gtactgccga tgagctcaag
    10981 gtccagctgg gcggtgagcg tctcgcgatc agcgtcccgg tgggaaccga tctcaatctc
    11041 gccgggaggg cattgctctc ggccgttcat accaatgacc tttccgagag cgatctcgtc
    11101 gtcgataccg atgcccacca gatgacggta ccgtttccag ccgggtcgcg tggccttgcc
    11161 gaggcggtgc gcaagctgga tgcggcaggc ctggagatca cggacttcag cctgcggcgg
    11221 ccaacccttg atgacgtgtt cctgacattg accggacacg tggcggaaga cggtgaagat
    11281 gccgcgaagg agaatgcagc atgagtgagc aaacattaac cctgccagcg gccccgatgg
    11341 cacagccgcg ttcgactacg gttgacttga agcctgtctc gcgcgggaag ttaggcgatg
    11401 ttctcattga ttcgctggtc ctggcgcatc gaagcctgct gcgggtgccg cgacagctgg
    11461 actggttgat tggcgtgacg gtcatgccgc tgatgttcct gctgatgttc cgctacatct
    11521 tcggcggcac tgtccaggcc acgatgccgg ctggtgagaa tgcagtcaat tacctcgtcg
    11581 tcgggatcgt cctgcagggc attgtcttcg gcggcatgaa caccgcgttg gggctcgcga
    11641 cggacgccaa ggaaggattg atggatcgct tccggtcgct tccaatgtcc cgcgtctcgg
    11701 tgctcctggg tcgcgtgctg gccgacatgg cgatcgcggt ctttgtcacg acgatcacgc
    11761 tgctcgtcgg tctgctggtc gggttccggc caaccggtga cttcatgaac tggctcgcgg
    11821 cgatcgggat gctattgctg gttgccttcg ccatgagctg ggtcggggcc gcggttggcc
    11881 tttggctcaa gaccatcgag gccgtgaact ccatcgggtt cagcctgata atgccactga
    11941 ccttcgtcag cagcgccttc accaggccgg agttcatgcc gtcgtggctg caggggttcg
    12001 ccaagaacca accgtttacg ctggcggtag acaccactcg aggactcctg accggttatc
    12061 cggaggttgg taacaagggt tggatcgcgg tcgcatggct ggttggcacg ttggtggtga
    12121 tgattccgtt gtccgtttgg ctgttcgagc gtcgcgcgaa gtcctagccc attcccgttc
    12181 ccgctacccc agccctcggt tgtagcgaaa acagatatac cggatactcc gttcctggag
    12241 tatccggtat atctgtgcaa tggcttgagg agcgtgggaa tgagttcgaa ggatcggcgc
    12301 cctgaagggg cgcaggcggc agagatctgc agttccatca tctggatggg acacctgatc
    12361 gagcgcttcg cgaacacccg gctgccggag gcgaacatcg cccctgccat gtcactgccg
    12421 cgggccaacc tcctctttgc agtgcactcc gcgcagcgga atggcggctc cacccgaatg
    12481 gtcgacatcg cgttcgacct cggtgtcacc gcccgcacga tcacgacgat ggttgacgcc
    12541 ctggagcagc aagggctgat cgttcgtgtg cccgatgcga aggaccgccg agcgatccag
    12601 ttggagatga ccgatgcggg ccggtccttg atggagccgc tggcacaggc cgtggaatcg
    12661 gcgagcgagg tgatcatgag tccgctcagt cgctccgagc gggagacact gcttcacttg
    12721 atgacccgcc tcattgagcg agacatgggc gactgagact tatcagggtg gacgcgttgg
    12781 gtaaactagc actcgatatg acgtggtcgc gcccggatca tgtcgccacc ccaggagacg
    12841 cccatcgccc catgcccgat ccaacttcct ccgcaccgga atcagcaact caggtcatgc
    12901 tttcgctgct cgatcagttg aggccggatc agcgcgtggt agtggcgacg ctcaccgaag
    12961 cattcgcgtc ggcgggagca gagctctatc tggtgggcgg gccggtgcgg gatctcctgc
    13021 tggagcgccc ggtgcctaac gatcttgatt tcgcgacgtc catcctgcct gagcgcacga
    13081 aggagatatg tgcgcggatc agtcacgcgg gggtctatga tgtcggcgaa aagttcggca
    13141 ctatcggtat cgtgatgcca gccggggtgg cgacaatccc ggttgagatc acgacctatc
    13201 gcagtgagca gtacgaggcc ggaagccggc aaccggaggt agccttcggc tcacgcattg
    13261 aggatgacct cgcgcggcgg gatgtcaccg tcaacgcgat tgccatcgat accggatccg
    13321 ggcaggtcgt cgatcccttc tatggacaag ccgatctggc catggggctg ctgcgtgccg
    13381 tgggagatcc cgacgaacgg tttgccgagg acccactgcg cctgctccgt gtcgcccgct
    13441 ttgtctccca gctcgggttt gccgtcgagt cgggaaccaa ggcggcaatg acccggcagg
    13501 cgaagacgct ggaaacgatc agccgggaac gaatcctcgc cgagctgaca aagctcctct
    13561 gcggcgacta cgcaggccac gggcttgatg tgctgctgga aaccgggctc ttcatagtcg
    13621 ccatgccgga gctgctgccg cttgaagggg ccgcaagcaa tcatcctggc gttcatcgtg
    13681 agaaggatct ctgggagcac accaaaagag tcatcatgca ggccccggca cggccaacgg
    13741 ttcgctgggc ggccctgttg catgatgccg ccaagccgct gacacggtcc gtggacagcc
    13801 ggggtgaggt ccacttcttc gggcatgagc gggttggcgc tgatctcgcc agcaagctac
    13861 ttcggcgtct caaggccgac aagcggaccc aacaggacgt tcgtcggttg gtcgaattgc
    13921 acctccgtcc cgcttcgtac gatacagaga cgtggactga cagcgcagtg cgtcggctgg
    13981 cgctggaggc aggagatgtc ctgcacgacc tgctcgatct cgccgcggcg gatgtcacgt
    14041 ccggacgtgc ccacaagcag cgagcggcgg cccggcgcgt tcaggggttg aaggatcatc
    14101 tggcgcggct ggaggaacag caggcgctcg acgagctcca gagcccgctg gacggcaacg
    14161 agctgatggt gatgttcgac aggccgcccg gtcgctggat cgccgaggtc aaggatcacc
    14221 tgcgcgagct ggtaatcgac ggtgagctcg cctccgacga gaaggagcgg gccgccggga
    14281 tcgcccgcga actgctggca ggcaactgat ggtcaacatg tcggcttttg gcaggggaga
    14341 ctgtcgtgat ctccagtacc actgcgggca gcagaaaccg atccagcagg catattgtct
    14401 gagggggtga gatatctgtc ggtacatccg cacgttccgc caaggatgcc gttcgcaggc
    14461 agtacgggtc tgtcggcgac gcctacgtca ggagccccgg ccatgcgacg ggaactgacc
    14521 tggaccggat ggtgatcctg gtgcagccag ccccggacga aacactgctc gacattgcga
    14581 cgggcggcgg gcatgtcacc cgtgtctttg ccccgcatgt cgcatcggtg gttgccaccg
    14641 acctgacacc cccgatcctg gagcacgccg cgaaggcgtt cgcgggctgg ggtatcggaa
    14701 acgtcacgac cgccgtagcc gacgccgagg cacttccctt cgacgatgcc tcgttcgata
    14761 tcgtgacctg ccgcatcgcc ccgcatcatt ttccggatcc agcggcgttc gtgcgtgagg
    14821 tggcacgcgt tctccgcctg ggtggccgtt tcgtgctggt cgatagcacc gtgcccgagg
    14881 gggaggacgg cgcactcttc aacaggttcg agaaactgcg ggatccctcg cacgttcgct
    14941 ccctgacagc aggggagtgg acatccctcc ttggcgatgc gggattctcg atcgaggcta
    15001 tcgaaccctt tatgaagcgc catgacttcg ccgactggac atcccgttcc cggacgagcg
    15061 cagaggatcg tgaggagctg acccgaatga tcctcggtgc gccgggcgtg ttcggcgcgg
    15121 agacggcccc ggacgatcca gggaccgtgc tggcgttcac ggacacgaag acactctttc
    15181 tcgggacgag gtgatgcacg gtggtcggca cggatgcgat catgaagcca ccgtctcctg
    15241 tggccggatc agcaccgtcc cggcgaggat gaccaccccc agaatcccaa acatcagcga
    15301 cagcaatcgc ccgtcaatca cctggagttg cttgcggata gagacactgc cttggtcgag
    15361 atcaacgtcc tctcatcgga gacctgatac ttcaccctgg cgcaagccga cggagagcac
    15421 cactgcatgg agtgcttcca tgcggtcgcc atgcagagtg acgatgggac gcgacgctac
    15481 cgttttgcaa agtgtcttgg gctgatcgca tctaactgtg gtggattcac gagcttcggt
    15541 acattacgcg ggatcaagtt tcactttgtg ggcaccgggc acgagcaaca gcggcgtagg
    15601 acatcaccgg gagccgacgt gtgccggctc ccactctccc actatagagg agcatgctgg
    15661 aggaacctat ggtcactatc cgtcccattc ggcccgatga tgagtccttc ctctgggaca
    15721 tggcgtggga ggcggcagcc gtctcaccag agatgcgtgc actgggacgg aacgcgacgc
    15781 tcgctgtgcc cgaaatccgt cgatacctcg aagcgtgggg acgtcctgga gacgcgggag
    15841 tggtggccgt cgatgagaac aatagacggt tgggagccgc atggtatcgg ttgttttcgg
    15901 ccgacgccaa ggggtatggc ttcgtagccc ccgatatccc ggagctgtcc atcggcgtca
    15961 gccctgcggc gcgtgggcgc ggtgtaggaa gcgctttgct gaacgccttg ctagcgacgg
    16021 cgcacgacga ggggcagcga ggtgtcagcc tcagcgtaga ccggcagatg tctgctcgcc
    16081 ggttatacga gcgccacggg tttcgcgatg tcggtatctc caacccagac gacacctcga
    16141 tcacgatgat ggtcacgctt tgacagcggg gccggcacac gtcggccctc atcgtggaag
    16201 gccagggcac acgatcacga cgcctcacgc cgcactgaat ccatggagat ccccggaaga
    16261 ccgggagaag atacggacca ccgagccgat gatcgtgctt tggtacaaca cggccgtcga
    16321 caacggtccg tgccccatgt gtggcgagcg caacgatcca gaaatcggtc tcgaacgctt
    16381 cccggtatgg aatggaacat tcatcctcac gccgctccgc gacaagtgcg ggcaacgttc
    16441 cgccctggag ttcaagtaat accggggggt caagccaccg gcctacgtca gcccgggacg
    16501 acggtacacc actgaagatc tgcgcgagct caccgtctat gccgctatct ggggaaagtg
    16561 accgggtcag aatcgacgac ggcccctgct tcctgcgggg gccgttgttg tgtcccgtaa
    16621 cggtgccccc gctgcatcca cccaatccac cccttacacc cgagccatgg cgttaggtac
    16681 gtcgttcctg aagattcggt aggccagttc ggtagcccta ccgatgtacc gccatgcctc
    16741 gatccgcaca ctaagacgta ggagatgcag cagggagccc gagtgaggac cctccaccga
    16801 cgcatccacg atcacgacgg gaggcatcca cgggtatgaa ccacatcgat ccggtcacca
    16861 acgtcgaatt gcaagatgca cgcattgctt cacatcaatc ggggatgacc cctggaaagt
    16921 acaccaccat gaatctcgtc gataccgtca ccagccacaa ttcgaccagc atgaaccggc
    16981 gctcactgct caagctgatg ggcgtgggag tggctgccct gtccgttggt gccgtcagca
    17041 ccgcggagcg cgccggggcc gagcatacgt cgcagctcta cacggtgagc actgcggcga
    17101 acttccggac cggtcccagc accagttacg caatcatctc ggtcatctcc aaggggacca
    17161 cctttaccct caacgggcag acccagaacg gatacgccag catcacctat cagagccgca
    17221 gcggctgggt cctggcatca cttgtggtcg cggcagggtc gtccggctcc caaccaactc
    17281 cggtgatctc cggtgaagcc tggaccatct ccgccgtcaa tctccgctcc ggtccctcga
    17341 cgagccacca agttctgcgg gtagttccca acgctgcaaa gatcggtgtc agcaccaacg
    17401 tgcagaacga cttccggtat gtcacctatc aggggcagac cggctggatg gccgatgcct
    17461 atatcagctt caccaatggc ggcggggatg accagggcgg caactacaag acgacgacga
    17521 ccgcgctgaa cctgcgggct gagccgagca cctcggcgaa gatcctgacc gtcatcccgg
    17581 agggcgcccg ggtccaggta ctgcactcgc aggctggaca gtacgtcaac gtcaactaca
    17641 acagtctgca gggctgggta gccctcgcct acctgaaata acccgtgtgg gagcggggga
    17701 tccccgctcc cctcccgtcg agaggaacga ggcaccatgt acgcccacga atacaaactg
    17761 gaactccagg ctacagccat catcgacacg tatcaccgag aagcgatccg ggcagagcag
    17821 cgtcgcctcg tccaggcagg acgcccaagc cacatcgcga cagcggccct gggtcatcga
    17881 gatatcaacc ttgatctgcg ttccacaacc caaggagcaa tcacaatgtt ttccgtacca
    17941 gtccgtttcc tttctcgtgg gctgtcgcgt cgcacgacgc tcgcggccca gatcctcacg
    18001 atggtggtgg tgctggtgag catcctcagc gtcgggggcg gttctacagc agcacagggg
    18061 acgagggttc gacatacctc gacgacctcg gcgatcgctg ccggagaagc ctgtttcacc
    18121 ggcttggcgg actgtagctg ggtcgatgtc tacatcgggc ccggtaaggt cgcgggcacg
    18181 acggtgctct gcctggagat caacaccgga gcggtgtatg ag
//
LOCUS       DASYWM010000133         4878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_211612, whole genome shotgun sequence.
ACCESSION   DASYWM010000133 DASYWM010000000
VERSION     DASYWM010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211612"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..616
                     /locus_tag="VGR29_05470"
     CDS             161..616
                     /locus_tag="VGR29_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2073074.1"
                     /translation="MIASPSYGSRVFAVNAHSTPGARTAWHTHPNGQTIFVTEGVGLC
                     QRPGGPIEVIRPGDRVYFEPGEEHWHGAAPDRFMTHLAMLKVDEQGNTATWGKHVMDE
                     EYGQQPGCRGQRREVGDSSVHYQSSSRRLTNEGFRYAVVGRLCGMKSLA"
     gene            642..1235
                     /locus_tag="VGR29_05475"
     CDS             642..1235
                     /locus_tag="VGR29_05475"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEV2073075.1"
                     /translation="MRNDRMGNATKTSNDSTVQTIYTIGHSTRPIEEFIDLLQRNGVS
                     TLVDIRTIPKSRRNPQYWHDTLVASLEDAGIVYRHLPGLGGRRKPLQDSINTAWRNHS
                     FRGYADHMQTEEFHEALGELIALGAGSTTAIMCSEAMPWRCHRSLVGDALLVREVEVL
                     DIISATSVRPHLLTPWAEVQGRTITYPGGDADPDAVG"
     gene            1435..2232
                     /locus_tag="VGR29_05480"
     CDS             1435..2232
                     /locus_tag="VGR29_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2073076.1"
                     /translation="MSRLATLRQLSPHLFIVYSEFPHRDSGNVYLITGRIPTLIDCGS
                     SRSAPQLRQNLAHLGIDVRDIAQVIATHGDCDHLQGYHELRRDHPNLQLHLHPADWSL
                     VQNSNPYRNANYLYSNPFEPIAPGQCLSLVDGQRIAAGDGELRVIHTPGHTEGSVSLY
                     GEINGRNVLFAGDTVGGAMRSLEGADLWIWAQAARTWQQSLQRLVPLEIDWILNGHEP
                     AAGLPLSRGWFNRAVASFGKMMNPWFLLDDAADDDEPALNVQIREAV"
     gene            complement(2318..3274)
                     /locus_tag="VGR29_05485"
     CDS             complement(2318..3274)
                     /locus_tag="VGR29_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073077.1"
                     /translation="MEIIIVILVLVVLLILFGGAGYFFVRETGWDNARLWLTGPRQLR
                     GIITPADDDTTRDAPTSAPERQQRPDPRPAQPGRMPLALDESALRSLREELQGELTRA
                     AGITRDFDARLTRLEADLSTSLHLPEEIGRTVQDVEVRTRRRLTKLRSEVRTTRVVES
                     SFGQRRSEALSDLYRKLAQVESTLAAVVNPMLLPGEPLNVPEELYDDTLEWSNWSDVG
                     ERAFSFGQVFNQSRFLLEPQLADQIERFIATFRQALTDTVYPVVQNEGRTKAQIAQMR
                     HGLGTIIEALPPVRREIEAAYRVTSLASMGDDDDDDDDDFDA"
     gene            3467..>4878
                     /locus_tag="VGR29_05490"
     CDS             3467..>4878
                     /locus_tag="VGR29_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV2073078.1"
                     /translation="MAIFRRSTPSDAPNPVTPPPTFFDRTERAEYETASVLQAELAAR
                     LQGEVRFDRVSRMLYSTDASNYQIDPVGVVVPRTMDDVIGAMDLAASHDVSILPRGGG
                     SSLAGQAVGSALVIDTSKYLNKVLTFNPEQKTVTVEPGINLDALNRQMKQHGLMFGPD
                     PSSANRATVGGVVGNNSAGAHSILYGMTADHVVSARVQLGTGETVDLGPGTMEDHVKR
                     ATASDSTGRLLAKLLAFREKHSELIRRDFPPHWRRATGYSLNQFITGDDSFNPARLMA
                     ASEGTFGTLLNVTIGLEDLPERTALVLLQFEDLVTAMEATNTILEIKPSAIELMDRML
                     ISLTREQPGYANMISFIQGDPAGILAVEFYGTSERELQQKCDDLVMHLRRRGTPLMTD
                     PQVLLDPKQQANVWNVRKAGLGLLMSVRGDAKPIPVIEDVSVPVEHLPAFVAAVQKMV
                     AKHNTTAAYYAHASAGCLHIR"
BASE COUNT          928 a         1537 c         1486 g          927 t
ORIGIN      
        1 gcgtatccaa ggatctcgtc cccctgcgtc acgatcagca tgccgacgtt gcggccatcg
       61 ccgttataga gcgaattgaa gatatccgac gcctcgcgat gccattccca gtcgaagtgc
      121 ttttcaagga aggtcaacac ccggcgcagt ctaccttgat gtgatcgcat cgccttccta
      181 cgggtcgcgc gtatttgcgg tcaacgctca ctccactccg ggtgcccgca cggcgtggca
      241 tacccacccc aacggtcaga caatcttcgt cacagagggc gtcggcctgt gccagcgtcc
      301 cggcggaccg atcgaggtta tccgtcccgg cgaccgggtg tactttgagc ccggtgagga
      361 gcactggcat ggcgccgccc cggaccgatt catgacccac ctcgcgatgc tcaaggtgga
      421 cgagcaaggc aataccgcga cctggggcaa gcacgtcatg gatgaggaat acggccagca
      481 acccggttgc cgcggtcagc gacgagaggt cggcgatagc agcgttcact accaatcctc
      541 ctctcgtcgt cttactaacg agggattccg gtatgccgtg gttggtcgat tgtgcggcat
      601 gaagtcactc gcctgaagcg ctccggccgg tggacgtcat catgcggaat gaccgcatgg
      661 ggaacgcgac gaagaccagc aacgacagca ccgtccagac gatctacacc attggacatt
      721 cgacgcggcc gattgaggag ttcatcgatc tgctgcagcg aaacggcgtg agcacgctcg
      781 tcgatatccg gacgatcccg aaatcccggc gaaatcccca atactggcac gacacactcg
      841 tggcgagttt ggaagatgca ggaatagtct accgccacct gcccggtctc ggtggccgtc
      901 gcaagccatt acaggactca atcaacacgg cctggagaaa ccactcgttc cgcggctacg
      961 cggaccacat gcagacggaa gagttccatg aagcgttggg tgagctgatt gccctcggcg
     1021 ccgggtccac caccgccatc atgtgctccg aggcgatgcc gtggcgatgt caccggtcac
     1081 tcgtggggga cgctctgctg gttcgggaag tcgaggtgct cgatatcatc agcgcgacga
     1141 gtgtgcgccc acacctgctc acgccatggg ctgaagtgca gggcaggacc attacgtatc
     1201 ctggtggaga tgctgatccc gacgccgtgg ggtgaccgcg aaggcattta ggtgaaatgg
     1261 caagacataa tcatttactg ataccttctt ctcatatgac cgtacactgc caattacaag
     1321 tcggggaaac gctgatcttt acaaggcgag aaccggcgcc tagactctta agtatggcaa
     1381 ttgttctctc ggcagccgta cgccgcaaga gagtcaccag gcagaaacgc gcgcatgtcc
     1441 cgactcgcca ctcttcgcca actttcacct catctcttta tcgtttactc agagtttcct
     1501 catcgcgaca gcggcaatgt ctacctgatc actggccgca tccctacgct tatcgattgt
     1561 ggaagtagcc gcagcgcgcc ccagcttcgg cagaatctcg cgcatctcgg gatcgacgtg
     1621 cgggacattg ctcaggtcat cgccacgcat ggtgactgcg atcaccttca ggggtatcac
     1681 gagctacgtc gagatcatcc caacctacag ctgcatctgc accccgccga ctggtcgctg
     1741 gtccagaact ccaatcccta ccggaacgcc aattacctct acagcaaccc gtttgagcca
     1801 atcgcccccg ggcaatgtct ctcgctggtc gacggccagc gtattgccgc cggagatggc
     1861 gagctgcgcg tcatccatac tcccggccac actgaaggct ctgtctctct ctacggggag
     1921 atcaacgggc gcaatgtgct cttcgccggg gatacggtag gtggcgctat gcgcagcctg
     1981 gagggagccg atctgtggat ctgggcgcag gcggcacgca cctggcagca atcgcttcag
     2041 cgcctggtcc ccctggaaat cgactggatc ctcaacggac atgagcctgc ggccggcctg
     2101 ccgctcagcc gtgggtggtt caaccgcgcg gtggcgtcct tcggcaagat gatgaacccc
     2161 tggttcttgc tcgatgacgc agctgacgac gacgagcctg ccctcaacgt gcagatccgg
     2221 gaagcggtgt agggacgcat acggtcgcag ggatgcctgt cgcacgttct cgcgacccag
     2281 ggttcaccca accatcttgt cgcgcccgtg ggtgatccta cgcgtcgaag tcgtcatcat
     2341 catcgtcatc gtcgtctccc atgctggcga ggctggtgac acggtaggcc gcctcgatct
     2401 cgcggcggac cggcggcagc gcctcgataa tcgtccccag cccatgtcgc atctgggcga
     2461 tctgcgcctt ggtgcgtccc tcgttttgca cgacgggata gacggtgtcg gtcagggctt
     2521 ggcggaaggt cgcgatgaat cgctcgatct ggtcggccag ctggggctcc agcaggaagc
     2581 gcgactggtt gaagacctgg ccgaagctga aggcgcgctc acccacgtca ctccagttcg
     2641 accattccag cgtatcgtcg tagagctcct ccgggacatt gagcggctca cccgggagca
     2701 gcatcgggtt gacgaccgcc gccagggtgg actccacctg ggccagcttc ctgtagaggt
     2761 ccgacagggc ctcgcttcgt cgttggccaa acgagctctc caccactcgg gtcgtgcgga
     2821 cctcgctccg gagcttggtg agacgcctgc gggtgcgaac ttcgacatcc tggacggtcc
     2881 tgccgatctc ctcggggagg tgtagcgacg tgctcaggtc tgcttccaac cgggtgagcc
     2941 gagcatcgaa gtcccgggtg ataccggcgg cacgggtaag ctcgccctgc aactcctccc
     3001 gcaggctgcg aagtgcgctt tcatccagtg caaggggcat ccgtccgggc tgcgcagggc
     3061 gcggatccgg tcgctgctgg cgttccggag ctgacgtagg agcgtcccgc gtagtgtcgt
     3121 cgtctgccgg ggtgatgatc ccgcgaagct ggcgtggccc agtgagccag agacgggcgt
     3181 tgtcccaacc tgtctcgcgg acgaagaagt acccggcgcc accgaagagg atgagcagga
     3241 caacaaggac gaggatgaca atgatgattt ccatggacgg tctgtgcttt cacggcaact
     3301 ggtaagcgat gagggctccg ctgacgagct tgtgtgctga cagtataggg tttcggcgcc
     3361 cgtgccggtc cggattccgg ctgtgaggtc gcggtgctac actccaatcc attgcgcatg
     3421 tataccggaa cgatggctct gcctcgttcc gaaggagaca ttccccatgg cgatcttccg
     3481 cagatcgaca ccctccgatg cccccaaccc tgtcacgccc ccgccgacct tttttgatcg
     3541 aaccgagcgc gcggagtatg agacggcgag cgtcctgcag gcggaattgg ctgctcggct
     3601 gcaaggggag gttcgcttcg accgggtgag ccggatgctc tactcgaccg atgcctccaa
     3661 ctaccagatc gacccggtag gcgtggtcgt cccccgcacg atggacgatg tgatcggcgc
     3721 gatggacctc gccgcaagcc atgatgtgtc catcctgccg cgcggcggcg gatcgtccct
     3781 ggcagggcag gcggttgggt ccgcgctggt gatcgataca tccaagtacc tcaacaaggt
     3841 gctgacgttc aaccccgagc aaaagacggt caccgtggag ccaggcatca atctggacgc
     3901 gctgaatcgc cagatgaagc agcacggtct catgttcggc cccgatccct catcggcaaa
     3961 tcgggcgacg gtcggcggtg tggtggggaa taactcggcc ggcgcacact ccatcctcta
     4021 cggcatgacg gcggatcatg tcgtctccgc cagggtgcag ctcggcaccg gcgagacggt
     4081 ggatctcggg cccgggacga tggaggacca cgtcaagcgc gccacggcga gtgattccac
     4141 gggccgcctg ttggcgaagc tgcttgcctt ccgcgagaag cacagcgagc ttatccgtcg
     4201 cgattttcct ccacactggc gacgcgcgac gggatactcc ctgaaccagt tcatcaccgg
     4261 ggacgacagc ttcaaccctg cccggctgat ggcagcctca gagggaacgt ttggcacgct
     4321 cctgaacgtt acgatcggtc ttgaggactt gccggagcgt acagccctgg tgctgctcca
     4381 gttcgaggac ctcgtgacag ccatggaggc aacgaacacg atcctggaaa tcaagccctc
     4441 cgcgatcgag ctgatggacc ggatgctgat cagcctcacg cgcgagcagc cggggtacgc
     4501 caacatgatc tccttcattc agggagatcc cgccggtatt cttgcagtcg agttctatgg
     4561 cacgtccgaa cgcgagctgc agcagaagtg cgacgacctg gtcatgcacc tccggcggcg
     4621 cggcaccccg ctgatgaccg atccccaggt cttgctcgat ccgaagcagc aggcaaacgt
     4681 ctggaacgtg cgcaaggctg gtctcggtct cctcatgagc gttcgcggtg atgccaagcc
     4741 gatccccgtg atcgaggatg tctccgtacc ggtagagcac ctgccggcct ttgtcgctgc
     4801 cgtgcagaag atggtcgcga agcacaacac taccgctgcc tattacgcgc atgcctcggc
     4861 cggatgcctg catattcg
//
LOCUS       DASYWM010000134         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_492643, whole genome shotgun sequence.
ACCESSION   DASYWM010000134 DASYWM010000000
VERSION     DASYWM010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492643"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VGR29_05495"
     CDS             <1..424
                     /locus_tag="VGR29_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEV2073079.1"
                     /translation="DLTFTYDKMKRACEAIDHGARFIATNADDRLPTETGYQPGAGAV
                     VAGIERATHISPIVVGKPEPLMMIKGVERLGCEPGEAVMVGDRLDTDIAAAYRAGLMT
                     ALVLTGVATRGDLGRAEVLPDYVFADLPALLQGIVGHG"
     gene            512..1465
                     /locus_tag="VGR29_05500"
     CDS             512..1465
                     /locus_tag="VGR29_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV2073080.1"
                     /translation="MHRTDTPIEVHGDLRLATAPVNWNNNDIPGWRPVVPFPEILDRM
                     QEAGYSATEYDSSFGCDPAILRSAVEDRGMTWCGSYQWVDFLDTEGLDAIIRSLTPTL
                     ELLDAIGCRNLLVADALRPHRVSMAGRVPADGSASLSSDDLRRLAEGAHRLAESAAAH
                     NIAIHYHNHVGSWIEAPHEVETLLTHLDSALVDVCFDTGHYAYGGGNPADFIAHYHER
                     IGYLHLKDVDTDAVQNARRQSLTFIEALKHHVFSPVGAGSADIPAILDVLVSSDFDGW
                     VVIEQDTCEGDATVTARRNLDFIRAWLQRTVESQHTKRGPA"
     gene            1462..2337
                     /locus_tag="VGR29_05505"
     CDS             1462..2337
                     /locus_tag="VGR29_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV2073081.1"
                     /translation="MKSITRPTHETKTIPELNALSRQTRRLILQSVHTAGAGHIGGPL
                     SVTDILVSLYFNELHIDPSNPSAEDRDRLILSKGHSSIALYTVLALRGFFPVDELSTF
                     DQIDSRLQGHPDLTKLPGLDMSTGSLGQGLSPGIGMALGARLRGSDFRTWVILGDGEL
                     QEGQIWEAAFVASRYRLANLTAIVDANGLPQFGWPHESGHTLDVPIDDPAGKFRAFGW
                     NVIEVDGHDHQALLDSWAAAKNHTDGPTCIVAKTIKGKGVSFMEGDYLWHAQVPTPDD
                     IANAGLELVDPEEVK"
     gene            2334..3290
                     /locus_tag="VGR29_05510"
     CDS             2334..3290
                     /locus_tag="VGR29_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEV2073082.1"
                     /translation="MSLTEIAMRDAWGQGLVDLAGTYANLVVLDGDLANSTKADMFAK
                     AYPDRFFEMGIAEQNMLGVAAGLATTGYVPWISTFAAFLAKRALDQIRVVISQPGLNV
                     KMAGSYTGILTGKTGKTHQAVQDIAVFRAMPHVVTIVPGDAMELRGAMAAMMEDDRPT
                     YLRLTRDPSPLIFGDDYTFEIGKGHLLRAGGDIGIISTGVQTVRALAAADLLATSGVR
                     ASVLHLSTIKPLDTDAIIDLADRTDAIVTAEDHSIIGGLGGAVAEVLGEHRPTRMHRI
                     GIRDTYGESGPNDALLEKYGLTDHHIAEAAESLLAMHSLEGR"
     gene            3287..>3554
                     /locus_tag="VGR29_05515"
     CDS             3287..>3554
                     /locus_tag="VGR29_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2073083.1"
                     /translation="MNSLPRVALIGCGRIGQVHARTLTSLRHLCDFAVIDDWNVEAAQ
                     VTARLLGVPRATADSEAVIADPDIDAVIIASSTETHAPYIELAAS"
BASE COUNT          722 a         1095 c         1041 g          696 t
ORIGIN      
        1 cgatctcacc ttcacctacg acaagatgaa gcgagcctgc gaggcgatcg atcacggtgc
       61 gcggttcatt gccacgaacg ctgatgatcg cctgccgacg gaaacgggat accagccagg
      121 ggccggagcg gtggtcgccg ggatcgagcg agccacacac atctcaccga tcgtggtcgg
      181 caaaccggag ccgttgatga tgatcaaggg tgtcgaacga ctaggttgcg aacctggaga
      241 ggcggtgatg gtgggcgacc gcctcgacac cgacattgcc gccgcctatc gtgccgggct
      301 catgacggca ctcgtgctca ctggcgttgc gacacgcggc gatctgggcc gcgcagaggt
      361 tctcccagac tacgtatttg ccgacctgcc cgcactcctt cagggtatcg tcggtcacgg
      421 ctagttaccg ttgcaaaaat ttgactcccg cacaggacgc taatacgata ccggttatgg
      481 aaagaaccac tgtgataggg aacgccatct aatgcaccgg accgacacgc caattgaggt
      541 acacggagac cttcggctcg ccacggcgcc cgtaaactgg aacaataacg acatccccgg
      601 ctggcgacct gtcgttccct ttcccgagat tctcgatcgc atgcaggagg ccgggtatag
      661 cgcaacagaa tacgactcct ccttcggatg cgaccctgca atactccgct cggcggtcga
      721 agaccgggga atgacgtggt gtggttccta ccaatgggtt gattttctgg atactgaagg
      781 cctcgacgca attatccgct ccctgacgcc gacactggag ctccttgatg ccatcggctg
      841 tcgcaacctg ctcgtcgcgg acgcacttcg gccccaccgg gtctcgatgg cgggccgtgt
      901 gccagccgac ggttcagcgt cattatctag tgacgacctc cgcaggctcg cggagggcgc
      961 tcatcgattg gccgaatcag ctgctgccca taacattgcg atccattatc acaaccacgt
     1021 cggaagttgg attgaagctc cgcacgaggt agagacgctt ctcacccatc tcgattccgc
     1081 cctggtggat gtgtgtttcg ataccggcca ctacgcgtac ggcggcggaa accctgctga
     1141 tttcattgct cattatcacg agcggatcgg ctatctgcac cttaaggatg tcgatacgga
     1201 tgccgttcaa aacgcccgca ggcaatcact caccttcatc gaggcactga agcaccacgt
     1261 tttcagcccg gttggagctg gatccgccga catcccggcg atccttgatg tcctggtttc
     1321 aagcgatttc gacggctggg tcgtcatcga gcaggacacc tgtgaaggtg atgccacagt
     1381 gacggcacgt cgcaatctcg atttcatcag ggcctggctc cagcgcacag tggagagtca
     1441 gcacacaaag agaggaccgg catgaagtcc atcacccgtc caacgcatga gaccaagacc
     1501 attccggagc tgaacgcgct ctcgcgccag acacgccggc ttatcctgca atcggtgcat
     1561 acggcgggcg ctggtcatat cggaggaccg ctttcagtca ccgatatcct cgtctcgctc
     1621 tacttcaacg agctccacat cgacccgtcg aacccctcgg cggaggatcg ggatcgtctg
     1681 attctcagca aggggcattc gtcgatcgcc ctctataccg ttttggcgct gcgaggcttc
     1741 tttcctgtcg acgagctctc gaccttcgac cagatcgatt cacgactcca ggggcatccc
     1801 gacctgacga agctacccgg cctcgatatg tccaccgggt cactcggtca ggggctttcc
     1861 ccggggatcg gcatggcgct tggcgcccgg ctccggggct cggatttccg tacctgggtc
     1921 atcctcggtg atggcgagct gcaggaggga cagatctggg aggcagcctt cgtcgcgtcg
     1981 aggtaccgtc tcgcgaacct gaccgcgatc gttgacgcga acgggcttcc ccagttcgga
     2041 tggccgcatg aaagcggcca cacccttgat gtgcccatcg acgacccggc gggtaaattc
     2101 cgcgcattcg gctggaacgt aatcgaggtc gatgggcacg atcaccaggc gctactggat
     2161 tcctgggccg ctgcgaagaa tcacaccgat ggacccacat gcattgtcgc aaagacgatc
     2221 aagggcaagg gcgtctcatt catggaaggc gactaccttt ggcatgcgca ggtcccaaca
     2281 cccgacgaca tcgccaatgc gggacttgaa ctcgttgatc cggaggaggt gaaatgagcc
     2341 tcaccgaaat cgccatgcgc gatgcatggg gccagggcct tgtcgatctc gctggcacat
     2401 atgcgaacct ggtcgtcctg gatggggatt tggcaaactc aactaaggca gatatgttcg
     2461 caaaggccta ccctgaccgg tttttcgaga tgggtatcgc cgagcagaac atgttgggcg
     2521 tagccgccgg tctcgcgaca acgggatacg taccttggat cagcacattc gcggcattcc
     2581 tggcgaagcg agcgctggac cagattcggg tggtgatctc ccaaccgggt ctcaacgtca
     2641 agatggccgg ctcctatacc gggatcctga ccggaaaaac cggcaagacg caccaggcgg
     2701 tgcaggatat cgctgtcttc cgggcgatgc cacatgtcgt caccatcgtc cccggtgacg
     2761 cgatggagct ccgcggcgct atggcggcga tgatggaaga cgaccgaccc acctacttgc
     2821 gtctcacccg cgaccccagt ccactcattt tcggagacga ctacacgttt gagatcggaa
     2881 aggggcatct cctgcgtgct ggtggtgaca ttggcatcat cagcaccggc gtccagaccg
     2941 tacgtgccct cgcagcggcc gatctgctgg ccacttccgg cgtgcgcgcg tcggtactac
     3001 atctttccac gatcaagccg ctcgacaccg acgcgatcat cgacctcgcc gaccggactg
     3061 atgccatagt cacggcggaa gaccactcga ttatcggtgg gctcggtggt gcggttgctg
     3121 aagtcctagg agagcatcgc ccaacacgaa tgcaccgcat cgggatcagg gacacgtacg
     3181 gggaatccgg accgaacgat gctctcctgg agaagtacgg cctcactgac catcacatcg
     3241 cggaagcagc cgaatcactc cttgccatgc attccttgga gggtcgatga actctctccc
     3301 tcgtgttgcc ctcatagggt gcgggcgcat tggacaggtt catgcacgta ccctcacatc
     3361 actgcggcac ctgtgcgact tcgcggtgat cgatgactgg aacgtcgagg cggcacaggt
     3421 cacagccagg ttgctgggcg tcccgagagc tacggcggat agtgaggcag tgatagccga
     3481 tccggacatc gacgcggtca tcatcgcgtc gtcgacggag acgcatgctc cttatatcga
     3541 gctcgcggct tcgc
//
LOCUS       DASYWM010000135        10614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_568069, whole genome shotgun sequence.
ACCESSION   DASYWM010000135 DASYWM010000000
VERSION     DASYWM010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568069"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..432)
                     /locus_tag="VGR29_05520"
     CDS             complement(175..432)
                     /locus_tag="VGR29_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073084.1"
                     /translation="MSAAWLRNHGEGETVPLLHAFIEGRDRTREPGGVRLVPFAGSAF
                     SGTATNSCTPGDVTGKGMGYVRTREIRLARADNLDLEDGSL"
     gene            complement(599..1483)
                     /gene="nadC"
                     /locus_tag="VGR29_05525"
     CDS             complement(599..1483)
                     /gene="nadC"
                     /locus_tag="VGR29_05525"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV2073085.1"
                     /translation="MLNQTISGPTTFQPHVQAIIDLALSEDIGTGDVTSLATVPADRM
                     AEGVMLAKAEGVLSGIEVASQVFRTVDPAIVFTALRADGDQLSHRMEIATLSGPARSL
                     LTAERTALNLVQRLSGVATQTARYVAKTEGTNAHIVDTRKTTPGMRLLEKRAVMHGGG
                     RNHRFGLADGVLIKDNHLAAIGGPRRITEAIERARVLAPHTLRIEIEVTSLEGLREAL
                     DGRADIVMLDNMETSTMAEAVRIRDAHAHPALLEASGGITFERIPEIAATGVNLISVG
                     ALTHSSPALDISLDLLLT"
     gene            1803..2303
                     /locus_tag="VGR29_05530"
     CDS             1803..2303
                     /locus_tag="VGR29_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2073086.1"
                     /translation="MKCVLLTGMSGTGKSTVIKELSDQGYRAIDTDYDGLSELVAVPD
                     DELTGLEPGQDWVWREDRIQALLSTHDVDVLFLGGCSPNQGKFYPKFDHIILLSAPAE
                     ALVDRLATRTNNPYGKQPEEVARVLQLQHAIEPLLRATATHEIDTSIPLDQVVAKVLE
                     YADVSG"
     gene            2506..2808
                     /locus_tag="VGR29_05535"
     CDS             2506..2808
                     /locus_tag="VGR29_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073087.1"
                     /translation="MGIRIENTPHGAGPVIICDYCGTRIHAARDGNYEWAGLSTALGE
                     RMAIYFLHKWCSPAHEAQRGIVLDSMELAVMLPYLAQNLQLDFDEALRHAETLSEL"
     gene            3049..3483
                     /locus_tag="VGR29_05540"
     CDS             3049..3483
                     /locus_tag="VGR29_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2073088.1"
                     /translation="MAQTSTYLNLNGTTEAAFTFYKSVFGTEFDGLMRMGDVPPQEGQ
                     PELSNEDKQLVMNVQLPILGGHLLMGTDATESMGFTLNQGNNVYICLQPDTREDADAL
                     FASLSGGGEVEIPMMDMFWGDYFGSLVDQYGIRWMINCSSTT"
     gene            complement(3650..4186)
                     /locus_tag="VGR29_05545"
     CDS             complement(3650..4186)
                     /locus_tag="VGR29_05545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEV2073089.1"
                     /translation="MSGNTINEDSLVFIDPQSGFEIRPWPALRDAEAATILADSTGEG
                     SSEAGQRAIDEARAEAPDRVFAGLMGGQLVGVYTLRRDGMANRIGNIAVAKDHRRRGI
                     GRALLQDALRRSGRRPLVAETDEEGLGFYKACGFKMVGRRKHPSGTVRYRIGWHAPGL
                     RFKGGSTNSLTHKPVKDQ"
     gene            complement(4204..5541)
                     /locus_tag="VGR29_05550"
     CDS             complement(4204..5541)
                     /locus_tag="VGR29_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV2073090.1"
                     /translation="MSSSSEVLGGPNGSSSLTDAPPYEKSTLPNGVRVVTGPMTGVRS
                     ASLIFYYNVGSRVEPALIAGVSHFLEHMLFKGTTKRPDPMTISEEIESVGGMLNAGTG
                     RESTSYWCKVPSTHFELAYDVLADMLRNSTFDATELDKERKVIFEEIRAVQDVPEDLV
                     HDLIDVVVWGDDPVGREIAGSEETVSNITRDLMVDFWQRNYGAERLVVAAGGDIRHED
                     VVALTERYFGDLQPGNSVDTFVPTLVTQNERRVEVINRETEQAHLCLSFPALSYLDER
                     RYVQSTIEAILSSGMSSRLFQEIREKRGLVYSVYGYFRGYEDVGQGVVYAGTDVDRVQ
                     ETIEAIIGELKKLRDITVPAQELEHTKTLRKGRLLMSLEDSRSVASWVGGQEATYGEI
                     MTPEQIMERIDAVTAEDVQQLAGELIRDDRFNLALIGPYTSRDEFAELLSLDA"
     gene            complement(5917..6411)
                     /locus_tag="VGR29_05555"
     CDS             complement(5917..6411)
                     /locus_tag="VGR29_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEV2073091.1"
                     /translation="MATPEQRLNDLLAEQDAITLATAESCTGGHVTARITNIAGSSAY
                     FHGGVVSYTNAVKAHLLNVPETVLQNPGAVSEECARAMAEGARSQLDASIAVSITGIA
                     GPSGGTKRKPVGLVYIGIASPSGTDVECHQFSGDREEVIGAAAERALELLVEQVEATL
                     EHEQ"
     gene            6902..7576
                     /locus_tag="VGR29_05560"
     CDS             6902..7576
                     /locus_tag="VGR29_05560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV2073092.1"
                     /translation="MGEWLQVGPAGSRAYLAVPESGIGAGVLVLHAWWGLTSVFTEVC
                     DRLASEGYVALAPSLYAGGETTSSIAEAEALVAAQNRAPAEAEAVVHAAVDHLRELPA
                     VTGAQIGVIGFSLGAYWALHLSQVRPDDVSAVVAVYGTDDGDYSTARAAYLGHFAEHD
                     DFEPLEVVQALEARIRAAGREVTFHLYPGTGHWFVEPNRTDAYDAAAAELVWERTLDF
                     LGARLA"
     gene            7658..8137
                     /locus_tag="VGR29_05565"
     CDS             7658..8137
                     /locus_tag="VGR29_05565"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2073093.1"
                     /translation="MDLLDRLLDHDHWATIQLLDLSRGLTDEQLDQPFDIGHRTVRDT
                     FDHMILNVEFWTGSMVNKPVPYEPARTSVDDMLARHERSYATFAGLARTLRDEQRLDD
                     TFGDHFDEQMTFGGGVLHVILHNEDHRTEVLHILQRLGVPDLPEVDHGLWDFRRRGF"
     gene            8170..9249
                     /locus_tag="VGR29_05570"
     CDS             8170..9249
                     /locus_tag="VGR29_05570"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV2073094.1"
                     /translation="MESKPKDPNDMMAHQIRSLVAGHADESGFSGVCLVKRGDDILAH
                     EAYGLAHRGFRVPNTVHTRFDIASVTKVFTAAAILRLIESGEVTLETQIMPFLGIDGT
                     QISNDVTIFHCLTHTSGIADDADEEAGEVYEALFVDSPNYAIRQTADFLPQFAHKQPV
                     FPPGQGVRYNNCAFILIGLVIEKITGMSYRDYVRRNIFALAGMDGADFCAMDEVNASF
                     AEHYKRIDHDDGTIEWRKNIYSYPPVGSPDGGATVTAMDLDRFMRSVVSGTLLGEEGS
                     QLLQRPHVLVEQHDDCTLMNGFAFEFKLDTDGQVLRMNKEGFNAGVSSMLAYYSTIDT
                     TVVLLANIEYSVWTMLREIEALLFV"
     gene            9604..9918
                     /locus_tag="VGR29_05575"
     CDS             9604..9918
                     /locus_tag="VGR29_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEV2073095.1"
                     /translation="MEPLERKPTAKRLHQMDVETLSRVLHDLKTPLTVMYGRSQLLER
                     YILRGRILKAHECLSGLAAIEQAIQRMEAMLKNLMGRDQDEWFSSPTGRGMPDPDGGP
                     LL"
BASE COUNT         2088 a         3196 c         3234 g         2096 t
ORIGIN      
        1 cggcagcctg ctgggctgcc ggcatgaggc tggaaagcat caggctgaga accgcaagaa
       61 tcaatgccag cgttcccgcg ccaatatgcc tcgaccgttg ccgggtgaga gatgcctcac
      121 gacccaccga cgctacagag aaagccttca ccgccactcc ccttcgctct tacatcataa
      181 cgagccatct tcgagatcca ggttgtccgc ccgagcgaga cggatttccc ttgtccggac
      241 gtatcccatg cccttgccgg tcacatcacc gggggtacag gagttcgtcg ccgttcccga
      301 aaaggcactt cccgcaaacg gtaccaggcg cacgccgcct ggctctctgg tgcggtccct
      361 accctcgata aacgcatgga ggagaggaac agtttctccc tctccatggt tacgcaacca
      421 tgccgctgac acgcaccgcc cctgacaggc agggcgtcac cgtgccttca tgtaggtgta
      481 cgtacactaa acgtcaccgg gttccctggg agaagagggt ttcgaccgat cggaatcgac
      541 cgatgccaat gggcaccata ccacgactgg gagggcgctg tcccatgggc tgctgacttc
      601 aggtcaacaa caggtcgagg ctgatatcga gcgccgggga agagtgcgtc agcgcaccga
      661 ccgagatgag attgaccccg gtagcggcga tctccggaat gcgctcaaag gtaatacccc
      721 cggacgcctc cagaagggcg ggatgggcat gcgcgtcgcg gatgcggacc gcctccgcca
      781 tagtgctcgt ctccatgttg tccagcatga cgatgtccgc ccggccatcc agtgcttccc
      841 gcaggccttc gagcgaggtc acttcgatct cgatcctgag cgtatgaggt gccagcactc
      901 gtgcgcgctc gattgcctcc gtgatccgcc ggggaccgcc gatcgcggcg aggtggttgt
      961 ccttgatcaa cacgccgtcc gcgagaccaa accggtgatt gcggccacct ccatgcatca
     1021 ccgcgcgttt ttccagcagg cgcatgccgg gcgtggtctt gcgggtatcg acgatatgcg
     1081 cattggtgcc ctcggtcttg gcgacgtagc gagccgtctg cgttgcaaca ccagagaggc
     1141 gctgcaccag gttgagcgcc gtgcgctcgg ccgtcagcaa cgaccgggcc gggccagaaa
     1201 gcgtggcgat ctccatccgg tgcgagagct ggtccccgtc cgccctcaag gcagtgaaga
     1261 cgatggcggg atcgaccgtg cggaagacct ggctggcaac ctcgatgccg ctcagcaccc
     1321 cctccgcctt cgccagcatc actccctcgg ccatccggtc ggccgggacg gtcgccagcg
     1381 aggtgacatc accggtgcca atgtcctctg agagcgccag gtcgatgatc gcctggacat
     1441 ggggttggaa ggtggtgggg ccggaaatcg tctggttcag catggtggtt ttcctctgga
     1501 gagggccagc tttttgctgc ctgtacggga cacactatct cctcagcatg caacaggaga
     1561 gaccgcacag gcaagggatc taccccattg atccgttgac ggatgaaacc acctcaggaa
     1621 tgaggcttgg gtttggcagg gtctatcagc aagcaccaca tggcggaggg gtgtcgactc
     1681 cccacatata atcgagccac tattccgaag gagatcatgg ctcttcccac cctctggatg
     1741 gttcacatga catttccgtc acgggcgcac gtcttggcgt tggtgaaagt cttggtgatt
     1801 gcgtgaaatg tgtattgctc acgggcatgt ccggaaccgg caaatcgacg gttatcaagg
     1861 aactgtccga tcagggctac agggccattg atacggacta cgatgggctg tcggagttgg
     1921 tggctgtccc tgacgacgag cttaccggcc tggagcccgg gcaagactgg gtatggcggg
     1981 aagaccgcat ccaggccctc ctgtccaccc acgatgtcga cgtgctgttc ctgggcggct
     2041 gctcccctaa tcaggggaag ttctacccga agttcgacca catcatccta ctcagtgcgc
     2101 cggcagaggc gctcgttgat cgattggcga ccaggacgaa caacccctac gggaaacagc
     2161 ccgaggaagt cgcgcgcgtg ctgcagctcc agcatgccat cgagccgctc ttgagagcca
     2221 ccgcgacgca tgaaattgat acgagtattc cccttgatca ggtggtggcg aaggtattgg
     2281 agtacgccga cgtatcgggt tgatcacccc aaacatgcgt tgagcatggg atggcacatg
     2341 ctttctgttc gtccgcagcc gtctcatgcg gcccggtgtt gattccggag attcgcgagc
     2401 attacgaaaa acaacagttg cacctggggc ggctgggatg ccagtctaca ctttccgtgc
     2461 tagaatacga acatatgttc gatttcagga agtgtgaggg atacggtggg gattcgcatc
     2521 gagaacacgc cgcatggcgc cgggccggta atcatctgtg attactgtgg aacccggatt
     2581 catgctgccc gggatggcaa ctatgaatgg gcgggcttga gtacggcact cggtgagcga
     2641 atggccatct attttctcca caagtggtgc tcgcccgcgc acgaggccca acgggggatc
     2701 gttctcgact ccatggaact agctgtcatg ctgccctacc tggcccagaa tcttcagctc
     2761 gatttcgacg aggccctgcg ccatgccgag acgctgagtg agctctgacc gggcgggtcg
     2821 ctgtagcaac agaggcagtg tggccctggc ttggtcaatg tgtctccatg cgaccggtac
     2881 atccaattct tctcatgagc cacactccca caggttcact gccgagcagt agtggccgac
     2941 tgagagagga cgcaagtttg aagaagccag tgtgttacgg aaaccctatg ctggcgttca
     3001 cgtcgatcca tcacagccgg tgaggtcgca aagaacgagg agagacccgt ggcgcagaca
     3061 agcacctatc tcaatttgaa cggcacgacg gaagctgcat tcaccttcta caaatcggtc
     3121 ttcgggaccg agttcgatgg tctcatgcgc atgggcgatg ttccgcctca ggaggggcag
     3181 ccggagctga gcaacgagga taagcagctc gtcatgaatg tgcagttgcc gattcttggc
     3241 ggccacctgc tcatggggac ggacgccacg gagtccatgg gttttacgct caatcagggc
     3301 aacaacgtct acatctgtct ccagcccgac acccgggagg atgcggacgc gctctttgcg
     3361 tccctctctg gaggcggtga ggttgagata ccgatgatgg atatgttctg gggtgactac
     3421 tttggctccc tggtcgatca gtacggtatc cggtggatga tcaactgctc gagcacaacc
     3481 tagggagcct tccaagacgg ctggcgatgt gcacatgtct cgtaggggcg gacccaacag
     3541 gcacatccgc gggatgtgac cgggctcagc ccattgtgtc ctcccgctcc ctgcgcaggt
     3601 gtgccgtcaa tagccatgca aaccgcgata aggcgaagat cgcaggtggc tactgatcct
     3661 tgaccggctt gtgtgtcagg ctgttggtgg agccgccctt gaagcggaga ccgggtgcgt
     3721 gccagccgat ccggtagcgg acagtcccgg atgggtgctt ccgtcgtccc accatcttga
     3781 agccacaggc cttgtagaac ccaagcccct cctcgtcagt ctcggcaacg agagggcgac
     3841 gaccggaccg gcgaagggca tcctgcagca gtgcgcgccc aatgccacgg cgccggtggt
     3901 ccttcgccac ggcgatattg ccgatccggt tggccatgcc gtcccgccgc agcgtgtaga
     3961 cgccaacgag ttgtccaccc attaatccag cgaaaacccg gtccggcgcc tcggcccgcg
     4021 cctcatcgat cgcgcgctgg ccagcctcgg agcttccctc accggttgag tcagcaagga
     4081 tcgtggcggc ctcggcatcg cgcaatgccg gccatgggcg gatctcgaac ccgctctggg
     4141 gatcgatgaa gacgaggctg tcctcgttga tcgtgttccc gctcatgtac tgctcctgtc
     4201 cactcaggcg tcgagcgaca gcaattcggc gaactcatcc cgcgaggtgt atgggccgat
     4261 cagcgcgagg ttgaagcggt cgtcccggat caactcaccc gccagttgct ggacatcttc
     4321 ggcggtcacg gcatcgatgc gctccatgat ctgctccggc gtcatgatct caccgtaggt
     4381 cgcttcctgg ccgccaaccc acgacgcgac ggagcggctg tcctcgaggc tcatcaggag
     4441 gcgtcccttc cgtagtgtct ttgtgtgctc gagctcctgt gcgggaacgg taatgtcccg
     4501 cagctttttc agctcaccga tgatcgcctc gatcgtctcc tgcacccggt cgacatcggt
     4561 accggcgtag actacgccct gcccgacatc ctcgtaccca cggaagtagc cgtagaccga
     4621 gtagacgagc ccgcgcttct cccggatctc ctggaagagc cgtgagctca tgccggagga
     4681 gagaatggcc tcgatggtgg attgcacgta acgccgctca tcgagatagg aaagcgccgg
     4741 gaacgagagg cagaggtgcg cctgctcggt ctcgcggttg atcacttcga cacggcgctc
     4801 gttctgggtg acaagcgttg gcacgaaggt gtccacgcta ttcccgggtt ggagatcacc
     4861 gaagtaccgc tcggtcagtg ccaccacatc ctcgtggcgg atatccccac cggccgcgac
     4921 gactaggcgc tctgccccat agttgcgctg ccagaaatcc accatcaggt cacgagtgat
     4981 attgctgacg gtctcctcgg agccggcgat ctcgcgccca acgggatcat cgccccagac
     5041 gaccacatcg atgaggtcgt ggacgagatc ctccggaaca tcctgaacgg cgcggatctc
     5101 ctcgaagatg accttgcgct ccttgtcgag ctcggtcgcg tcaaaggtgg agttgcgcag
     5161 catgtccgcg aggacatcgt aggcaagctc gaagtgcgtg ctcggtacct tgcaccagta
     5221 agaggtagat tcgcgtccgg tgccggcgtt gagcataccc ccgacgctct cgatctcctc
     5281 ggagatcgtc atcgggtccg gtcgcttcgt cgttcccttg aagagcatgt gctcaaggaa
     5341 gtgcgagacg ccggcgatca gtgctggttc cacgcgggaa ccgacgttgt aatagaagat
     5401 caggctggcc gagcgcacgc cggtcatcgg cccggtgacg acgcgcacgc cgttcggcag
     5461 ggtggacttt tcgtagggtg gggcatcggt tagggaggac gagccgttcg ggccgcccag
     5521 gacttcagat gaagatgaca acgacgtttg ctcctgactc acgcgaataa tcgtgatgtg
     5581 agggtacagt acccatttcg cccccggcgt gccagaaatg agtgcacgag cattgcggga
     5641 atcgccagca gtggctcatc agcgggccgt tagcccttcg tcatcctgag catcgcgaag
     5701 gatcctacgc gaagcgtatt tgccacggtc ccgttgggaa ccagggcgtt ccacggcaac
     5761 aggtcttccg gatctgcgtc gtatagggtt cgttacaggt agttgcggcg ctccgaacga
     5821 cggggagttg tgctggagtg gtcggaagga tttgcccagc ccacgcatgg atgggcaacc
     5881 atagcagttt tccaaacgcc ctgaggcacg cctttctcac tgctcatgct caagtgttgc
     5941 ttccacctgc tcgaccagca gctcaagcgc ccgctcggcc gcggccccga tgacctcctc
     6001 gcggtcaccg gagaattgat ggcactccac atcggttccg gaaggggagg cgatgccgat
     6061 gtagacgagc ccgaccggtt ttcgcttggt gccgccgctc gggcccgcga tgccggtaat
     6121 gctgacagca atgctcgcat ccagttgcga gcgagcacct tccgccatgg cgcgggcgca
     6181 ttcctcgctc actgcgccgg ggttctggag caccgtctcc ggcacattga gcaggtgcgc
     6241 cttgaccgcg ttcgtgtagg aaacaacccc gccatgaaaa taggcggagc ttccggcgat
     6301 attcgtgatc cgtgccgtga catgccctcc ggtacaggac tcggccgtcg ccagggtgat
     6361 ggcgtcctgc tccgccaaga gatcgttcaa acgttgctcc ggtgttgcca tgaggtgtag
     6421 ggcctctctg tgtaagtctg gtcattcgat atttcgcgga ttccgccgcc cggagttgat
     6481 cggggtattg acgacccatg gcacggatac gatggtggaa ccgacgcgcg gtttgcatcc
     6541 ccgcggcatg ggcgctccac acctgtcggg agggcaaaac cggtgcccag gatgccaggt
     6601 gaacagagag tgacggtgaa acggccattc ctcacccatc ttacgccagc cgtaccgcct
     6661 ttcagccatc cgagcccggc ataaggaagg caaagggatc ctggagagca attccaggat
     6721 tgtgtacggg atgaaactca gcaccacccc agggagattg gggatctctc tttcattcct
     6781 gatggacccc gatgtaatgc aagtctcctt agtccagcac gagtccagca cgctttgcaa
     6841 acctggccgc cttcgctatc ctgtatcaga acagacgttc gatatacgta cggaggcaga
     6901 cgtgggcgag tggctacaag ttggaccagc gggaagtcgc gcctatctgg cagtgccgga
     6961 aagtggtatt ggagctggtg tgctggtgtt acatgcctgg tggggattga cgtccgtctt
     7021 caccgaggtg tgcgaccggc tggcatcgga aggctacgtg gcgctggcgc ccagtttgta
     7081 tgctggcggg gagaccacct cgtccatcgc cgaggctgag gcgttggtcg ccgcgcaaaa
     7141 tcgcgcaccg gccgaggcgg aggccgtcgt gcatgctgct gtcgatcatc tgcgcgagtt
     7201 gccggcagtg acaggtgcac agattggggt gatcggcttc tcactgggtg cctactgggc
     7261 actgcacctg tctcaggtgc gacccgacga cgtgagtgca gtcgtcgcgg tctacgggac
     7321 ggacgatggc gattacagca cggcccgggc cgcctacctc ggtcacttcg cggagcacga
     7381 cgacttcgag ccgctggagg tcgttcaggc actggaggcc aggatccgtg cagcggggcg
     7441 cgaggtgacg ttccacctct atcctggcac tggccactgg ttcgtggagc ccaaccggac
     7501 ggatgcgtac gacgcggcag ctgcggaact ggtctgggaa cgcacgctgg acttcctggg
     7561 tgcccggctc gcctgagcgc tccgcccgat caccggcgac cgttaccgaa acggcgcacc
     7621 gccccgcaca tcaccattcc cctgaggagg acatcgcatg gatctgcttg atcgcctgtt
     7681 ggaccacgac cactgggcca cgatacaact cctggacctg agccgcggcc tgaccgacga
     7741 gcagctcgat cagccgttcg atatcgggca tcgcaccgtg cgtgacacct tcgatcacat
     7801 gatcctcaat gtcgagttct ggaccggctc gatggtgaac aagcccgttc catacgagcc
     7861 cgcacgcaca tcggtggacg acatgctggc gcggcacgag cgctcctatg cgaccttcgc
     7921 gggcctcgca cgcacgttgc gcgatgagca gcgcctggac gacaccttcg gcgatcactt
     7981 cgatgagcag atgacgttcg gcggcggagt gcttcacgtc atcctccaca acgaggacca
     8041 ccgcaccgag gtgctgcata tcctccaacg cctcggggtg ccggacctgc ccgaggtcga
     8101 tcatgggctc tgggatttca ggaggcgggg attttagcca gccaagtcac acgacgggat
     8161 gcccggacga tggaaagcaa accgaaagac cccaatgaca tgatggctca ccagattcgt
     8221 tcgctcgtag cggggcatgc cgacgaatcc gggttctcgg gcgtgtgcct ggtgaagcgc
     8281 ggtgacgaca tcttggcgca cgaggcatac gggttggccc atcgtggatt cagggtgccg
     8341 aacacagtcc acacccggtt cgacattgcc tcagtgacca aagtctttac ggccgccgcg
     8401 atcctgcggc tgattgagtc gggcgaggtc accctggaga cacagataat gccgttcctg
     8461 ggcattgatg gcacccaaat tagcaacgat gtgaccatct ttcactgtct cactcacacc
     8521 tcgggcatcg cagatgatgc cgatgaggag gcgggtgagg tctacgaagc actcttcgtc
     8581 gacagcccca actacgcaat ccggcaaacg gccgacttcc tgccgcagtt tgcccacaag
     8641 caaccggtct tccctcctgg ccagggcgtc cgctacaaca actgtgcgtt cattctgatc
     8701 gggcttgtca ttgagaagat aaccggcatg tcgtatcggg actatgtccg gcggaatatc
     8761 ttcgctctgg ccggtatgga cggggcagac ttctgcgcaa tggatgaggt caacgcaagc
     8821 ttcgccgaac actacaagcg aatcgatcat gatgatggaa cgattgagtg gcgcaagaac
     8881 atctattcat atccaccggt tggttcaccg gacggcgggg ccacggtgac cgcgatggat
     8941 ctcgacaggt tcatgcgatc cgtggtgtcc ggaacattgc tgggcgagga gggcagccag
     9001 ctgttgcagc gtcctcacgt cctcgttgag cagcacgacg actgcaccct catgaacggt
     9061 ttcgccttcg agttcaagct ggataccgat ggtcaagtgc tccgcatgaa caaggaaggc
     9121 ttcaatgcgg gggtgagcag catgcttgca tactattcga cgatcgatac gacggtggtg
     9181 ctccttgcca acatcgaata cagcgtctgg acaatgctcc gggagattga ggcactgctc
     9241 tttgtataga acgtcgtgat aactgccttt gctatctccc ttgacagtag tggagtgatc
     9301 gccgcgacga tcagtcatat cgtctcggcg ccattcccga acccgacgcg caaggcgtca
     9361 gactttgtca ccgccgtttc tccgccaaca ccctcactcc agagccgagg ggtttggctt
     9421 gttcgccccc gctgccaaag actgcaggca ccttccggga aagcgataca gcaagcaggc
     9481 gcccgtttga ggatcacggg catctgtttg tctttgggct ccaccgcttc ctcccggcac
     9541 aggcattgca tgtgtctgct cccaaggggg aatgacgaca gagcagggaa ggaataatct
     9601 gctatggagc cgttggagag gaagccgact gccaaacgcc ttcaccagat ggatgtcgag
     9661 acgctgagcc gtgtattgca cgacctcaag actccgctga ccgtgatgta tggccgcagc
     9721 cagttgctcg aacggtacat actgcggggc cgaatcctga aggctcacga atgcctcagc
     9781 ggactagcgg cgatcgaaca ggcgatccag cggatggaag ccatgctgaa aaaccttatg
     9841 ggtcgtgatc aggacgaatg gttcagctct ccaactggta gaggcatgcc tgatcctgat
     9901 gggggaccgc tcctgtaata accccaggca atcttgacca ctcgtctgga tacgttgctc
     9961 aagagatgcc cggttcgctc aatcccacac ggatgttcgg cccatgccag gtggttaatc
    10021 ctcaataatg gccggctctg gaacagtgga ctccacccgg ggttccaatt cgatctcgag
    10081 gctgctcccc tatgaatgga gggttaacct ctttgtgtta ttgctgatac cgattcggtg
    10141 ctactgatac gattcacatc aaatctccga ctgttcgcgg aggacgactc catcgctcca
    10201 catattgcac cgatgtgaac agcgatggtg gtgtgggcga gattgacaac tgcccgaccg
    10261 tagcgaacgc ggatcaggcg aatacctatg gcaatgcccg aggtgatgct tgcgagacca
    10321 acaccacggt tgatgtcacc accgggacaa cagccgacgt accaccggta ccacgaccac
    10381 tggtacgcca gctagcacaa ccgtcggtgc aaccaccggg atcgcgcgtt ggttttcacg
    10441 gcgtcggcag tgctgtctac gggctcttaa agccagaagg acgcccaaag gaggcgtcct
    10501 tctgctggga ggaggtaact gagacctgga ctagcgcgtt gccgccagtt cggtctcggg
    10561 ggcggaagcc ggtagcagcg tctcccgctc ctggatctgc gcggccttcc cgga
//
LOCUS       DASYWM010000136         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_267689, whole genome shotgun sequence.
ACCESSION   DASYWM010000136 DASYWM010000000
VERSION     DASYWM010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267689"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..876)
                     /locus_tag="VGR29_05580"
     CDS             complement(64..876)
                     /locus_tag="VGR29_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2073096.1"
                     /translation="MTPSTRGYSRRIDLGKIALHVTQYDGNGPRLLLLHGIGSNCEGW
                     NPVIDDLALTFSPVTVDLRGHGCSDKPESGYLYIDYVSDIEGLLTALDWTSPLIIGHS
                     LGGIIALWWATRHPDRARALVAEDSALRSGEEFRPAFDGWLRLNALPYEELVAHYAAE
                     NPEWSPDMVESRARAMAGTRRGVFQELRDDSLAHDGKDRISELKEITSPVLLVHGDYQ
                     TGGMVHPEDIEALPRRLPDARTIRIPGGGHTLHRSSKEEFLAAAVPFLLAHA"
     gene            1170..2534
                     /locus_tag="VGR29_05585"
     CDS             1170..2534
                     /locus_tag="VGR29_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEV2073097.1"
                     /translation="MNRLNRRRFLALSSAGAASAVLVACGNEPPTAEELNPTQIPDVA
                     GAPPTLAPITSTPETARGGDSGAEGGEEAGGESADGGEEAGGGGQAAAGGGAEPVSVE
                     GGDLFFEPNEFSVAPGGTITLTNIGMLPHDMSVDEWGGVIIGPLEADQEGEYTVPEDV
                     QPGESFVFYCSIPGHREAGMEGTLTIVEAGAGGEAAAAPAEEEATPEEEAAAASGGEG
                     GGEIEVTVEAGDLFFEPSEFSIVPGGTIVLTNIGMLPHNMAVDEWGGVIIEELDAEGQ
                     GEYTVPDDAQPGESFEFYCSVPGHKEAGMVGTLTIAEAGGGGGAAAPAEEEATPEGGA
                     ATPMASPVDEAAPEEGGEQAAAAGGGGGGTEVAVEAGDLFFDPTEFEVAPGGTITLSN
                     AGVLPHDMAVDEWDGVIIGPLNGGESGEYTVPEDVQPGETFTFYCSVPGHKEAGMEGT
                     LTIV"
     gene            2724..>3995
                     /locus_tag="VGR29_05590"
     CDS             2724..>3995
                     /locus_tag="VGR29_05590"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEV2073098.1"
                     /translation="MTPTIHSDVQGLLRRSSGALRVRDGGVEVLDADAVRSELVDELV
                     WTAVFGEGDAQQVARWLIRVTAPEMGAYPASIHDLYMAAARREYSNITTPAINLRGMT
                     YDLARRVFRAAHATQSKQILLELARSEMGYTQQRPAEYASVILAAAIKEGWQGPVMIQ
                     GDHYQTNAKKYASDAVAEVAAVRDLAFEAIGAGYGNIDIDASTLVDLSHERLIDQQHL
                     NSRHTAELTMAIRDAEPAGLTISIGGEIGEVGKTNSTVEDLQAFMEGYEAELTKLGGN
                     LTGISKISVQTGTSHGGVVLPDGSIKDVAVDFDTLAKLSQAAKDFGLGGAVQHGASTL
                     PESAFDRFAEADAVEVHLATAFQNAIYDSEAFPQDLKDEIYAHLAANHADERKPDQTD
                     SQFYYTTRKRGFGPFKRQMWSMPEGTRETILD"
BASE COUNT          796 a         1092 c         1346 g          761 t
ORIGIN      
        1 ctcgcgccta tggatgtgcc tgcaagcacc agatggcggg agctccctgg gcagaagacg
       61 acgtcaggca tgtgccagta gaaatggcac cgcagccgcc aggaattctt cctttgaaga
      121 tcgatgcagc gtatgtccac cgcccgggat gcggatggtt cgtgcatccg gaagtcgcct
      181 gggaagggcc tcgatgtctt ctgggtgcac catgccaccg gtctggtagt cgccgtgaac
      241 aagcagcacc ggggaggtga tctccttaag ttcgctgatt cggtccttcc cgtcatgtgc
      301 caatgaatca tcgcgcagtt cctggaagac cccgcgtctg gtgccggcca tcgcccgggc
      361 acgggattcc accatgtccg gtgaccactc cgggttctcc gcagcgtagt gggcaaccag
      421 ttcctcgtat ggcaaggcat tcagacggag ccagccgtca aacgctggcc ggaactcctc
      481 gccactacgc agggcagaat cctcagcgac cagtgccctg gcccggtcgg gatgtcgtgt
      541 agcccaccag agggcgatga tgccgccgag cgaatgaccg atgatcagcg gacttgtcca
      601 gtcaagcgcg gtgagcagtc cttcgatgtc gctcacgtag tcgatgtaga gataacctga
      661 ctctggtttg tcggagcatc catggccgcg caggtcgacg gtcacgggtg aaaatgtcaa
      721 tgcaagatcg tcgataaccg gattccagcc ctcgcaattg gagccgatcc catgcaggag
      781 aagcaggcga ggtccgtttc catcgtattg ggtaacgtgc agggcaatct ttccgaggtc
      841 aatcctgcgc gaatagcctc gtgtcgaagg tgtcatgaga tcagctactc ctgggaagag
      901 gtcaacgata gtgatgattg agcaatgagg tgcgccggat attgtggacg attcgcggta
      961 ctcctgcagg gtgtccgatg aagttcgtgt cgtttcggga tacaatcgaa ccaaatgtgg
     1021 cgtgaagcgt aacgaggtgt tgcgcgtgcg tcctgtaagc gcgcgccatc atcactttgc
     1081 gagtgtccac tggccgggac tccacgtccc gtgccgtggt gctaagtgaa acacgcctgg
     1141 gtggacccgg tatggaggca acggcccgta tgaaccgctt gaaccgacga cgatttttgg
     1201 cgctttcttc cgcaggggcc gcgagcgcag tgctggtggc ctgcgggaat gagccaccga
     1261 ccgccgagga gttgaacccg acgcaaattc ccgatgtcgc gggggcacct ccaacgctgg
     1321 cgcccatcac gtcgaccccg gaaacggccc gcggtggcga tagcggcgct gaaggcggtg
     1381 aggaagccgg aggcgagagt gccgatggtg gcgaagaggc cggaggtgga gggcaggctg
     1441 ccgctggcgg tggcgctgag ccggtctcgg tcgagggtgg agacctcttc tttgaaccca
     1501 atgagttcag tgttgcccca ggcggtacca tcacacttac caatatcggc atgttgccgc
     1561 acgatatgtc cgtggacgaa tggggcggtg tgatcattgg cccgctggag gcggatcagg
     1621 aaggtgagta caccgttcct gaggacgtcc agccgggcga gtcattcgtc ttctactgct
     1681 ccattccagg ccaccgtgag gctgggatgg aggggacgct cacaatcgtg gaggctggtg
     1741 ctggcggcga ggccgctgct gcgccagcag aggaagaagc gactcccgag gaggaggctg
     1801 cagccgccag tggcggtgaa ggcggaggtg agatagaggt caccgtcgaa gcgggcgatc
     1861 tcttcttcga gccgagcgag tttagcattg tcccaggtgg caccatcgtt ttgaccaaca
     1921 ttggcatgct gccgcacaat atggcagtgg acgagtgggg cggcgtgatt attgaggagt
     1981 tggacgcgga aggtcagggc gagtacaccg tgccggacga tgcccagcca ggagagagct
     2041 tcgagttcta ttgctccgtc cccggtcata aagaagcggg catggtgggt acgctgacga
     2101 ttgccgaggc tggtggtgga ggcggggctg ctgcgccagc agaggaagaa gcgactcccg
     2161 agggtggcgc ggcaacacca atggcgtcgc cggtggacga ggccgcgccg gaagaaggtg
     2221 gcgagcaggc tgccgcggcc ggtggcggcg gaggtggcac tgaggtcgcg gtggaagccg
     2281 gagatctctt cttcgacccc accgagttcg aggtcgcgcc gggtggcacc attactctaa
     2341 gcaatgccgg tgttctgccg cacgacatgg cggttgatga gtgggacggt gtgatcatcg
     2401 gccccctgaa tggtggtgag agcggagaat acaccgtgcc ggaggatgtc cagccgggtg
     2461 agacgttcac gttctactgc tccgttccgg gccataagga agccggcatg gaagggacgc
     2521 tcaccatcgt ctagtctcac tacgcgttag gcttcctaaa gctcggtctg catcgcagac
     2581 cgagctttat gtatttgcta tcttcgtcgg ccaacatctt gcggaatcat gagatattgg
     2641 tgtcaaggac atggctggac gaacagggct ggcgtgcctg gcaccgcacg aggcgctgac
     2701 gccccgaagg aggaactgtg gcgatgactc cgacgattca cagcgatgtg cagggtttgc
     2761 tgcgacgatc tagcggcgca cttcgagtgc gtgatggcgg cgtcgaggtg ctcgatgctg
     2821 atgcggtccg ctcggaactg gtcgatgagc tggtttggac ggcagtcttc ggggagggcg
     2881 atgcccagca ggttgcccgc tggctgattc gggtgacggc gcctgagatg ggtgcgtatc
     2941 cggcctcaat ccacgatctc tacatggccg ccgcgcggag ggagtacagc aacatcacta
     3001 cgccggcgat caacttgcgt ggcatgacct acgatcttgc tcgacgcgtc ttccgtgccg
     3061 cccacgctac ccagagcaag cagatcctgc tggagttggc tcgctccgag atgggttata
     3121 cgcagcagcg acccgccgaa tatgcaagcg tcattcttgc cgctgctatc aaggagggat
     3181 ggcagggccc ggtgatgatc cagggcgacc actaccagac aaatgcgaag aaatatgcaa
     3241 gcgatgccgt ggcggaggtc gcggccgtgc gagatctcgc gtttgaggcg attggggcgg
     3301 ggtatggaaa tatcgacatc gacgcttcca cgctcgtcga tctttcgcat gagcgcctta
     3361 tcgaccagca acatctgaac tcccggcata ccgcggaact gaccatggcc atccgtgacg
     3421 ccgagcccgc cggactcacg atctcgattg gtggcgagat cggcgaagtt ggcaaaacca
     3481 acagcacagt tgaggatctc caggccttca tggaagggta cgaggcggag ttgacgaagc
     3541 tgggcgggaa cttgaccggg atcagcaaga tcagtgtcca aactggcacc agccatggtg
     3601 gtgtcgtttt gcctgacggc tcgattaagg atgtggcggt cgatttcgac acgctggcga
     3661 agctgtcgca agctgcgaag gacttcggct tgggcggtgc cgtgcagcat ggggcgtcga
     3721 cgttgccaga gtccgcgttc gatcgcttcg ccgaggcgga tgcggtggag gtgcatctgg
     3781 cgacagcatt ccagaacgca atctacgaca gcgaagcatt cccccaggat ttgaaggatg
     3841 agatatacgc gcaccttgcg gcgaatcacg cagatgagcg caagccggac cagaccgact
     3901 cccagttcta ctacaccact cgcaagcgag ggtttggccc gttcaagcgg cagatgtgga
     3961 gcatgccgga aggaacgcgt gagactattc ttgac
//
LOCUS       DASYWM010000137         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_605659, whole genome shotgun sequence.
ACCESSION   DASYWM010000137 DASYWM010000000
VERSION     DASYWM010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605659"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VGR29_05595"
     CDS             <1..211
                     /locus_tag="VGR29_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073099.1"
                     /translation="PTPTRQLEPPGFFALHYGRRTPISVILAHLLYGGILGAFYRLHR
                     SEPTRRHSASKLRNATTTLKGKIHD"
     gene            204..1172
                     /locus_tag="VGR29_05600"
     CDS             204..1172
                     /locus_tag="VGR29_05600"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEV2073100.1"
                     /translation="MTSRHSSKRPVLSRRHALQIAIGGSTGLALLGKSSALHAQPSPH
                     ASPTAEHYAHPEMLIDAHGLEQFRIDPVAILVGFMPEEDFDKAHIPGSVQLDWPALEV
                     TDTSDESIAQWREQASQTLASFGIAGERPVLAYDAGTLFAARLWWVLHYLGHEDKQVL
                     NGGLSAWQQATGEVATDATPAGATASSAVQSFQGQPRAGTLAQLEEVRASLDDPNVVI
                     VDARTPDEYSAGHIPGAVNINYPRNATSEMPKVWKPVDELREMYEEVGVTPDKHVIPY
                     CSTGVRSAVTFFTLRLIGYENVALYTGSWKEWGDHPDTPKPTGTEP"
     gene            1224..1895
                     /locus_tag="VGR29_05605"
     CDS             1224..1895
                     /locus_tag="VGR29_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEV2073101.1"
                     /translation="MKRVIVFDVNETLLDVGALDPIFARMFNDASVRREWFGQMLQSA
                     FVSTITGAYRDFGAIGISALKMVAERHGVSPGDEDLAALRDGMRQLPPHPEVREALEH
                     LHSSGFRLATLTNSTLEVSEAQLVNAGLHELMEQMLSADSVHRLKPAPEPYRMAAERL
                     GVTIGEIRLVAAHSWDIAGALHAGCAGAFVARPGQVLDPDGAQPDVIGSDIREIAERI
                     LASDQ"
     gene            2100..>3084
                     /locus_tag="VGR29_05610"
     CDS             2100..>3084
                     /locus_tag="VGR29_05610"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEV2073102.1"
                     /translation="MDARVGSWRLVVRESFWFIPAITTLLVVVLAVVLVRFDRTLRFD
                     QRSDLPWWLFGGGPEGIRAVLSAIAGTTITVTGVVFSITIVALQLASSQFSPLVLRTF
                     RADRGNQIVLGFFIATFTYSLLVLRSVLSPEQGTEGFAPVITSTGAIALALVSVTLLI
                     YFIHHAARSIQASTIIERASNDTLALIHHLFPEDIGEPGESYPSSALPQALATVVVAK
                     QGGYFQAVDGDTLFNLSEEQTLTVHVERIPGDYVLPESVLATVWPKTSVDQGMEEKVR
                     SAFLIGPERTLQSDVELGIRQIADIAIKALSPGINDPTTAMLCIDRLSEALV"
BASE COUNT          629 a          972 c          875 g          608 t
ORIGIN      
        1 accgaccccg actcgtcagc tcgaaccacc gggcttcttt gctcttcact atggaaggcg
       61 aacacccatc tcggtcatcc tagcccatct gctctacggc ggcatcctgg gcgccttcta
      121 ccggctccac cgctctgaac cgacacgtcg acattccgcc tcaaaactcc ggaacgccac
      181 caccacgctg aaagggaaaa tccatgacta gccgacactc atcaaaaaga cccgtactct
      241 cacgccgaca tgccctccag atcgcaattg gcgggtccac gggcctggcc ctcctaggaa
      301 aatcgagcgc cttgcacgct cagccatccc cacatgcgtc gccgactgcc gaacattatg
      361 cccatccaga gatgctgata gacgcccacg gtttggagca gttccggatc gacccagtgg
      421 cgatcctggt cgggttcatg ccggaagaag acttcgacaa ggcccatatt ccgggttccg
      481 tgcagctcga ttggcccgcg ctcgaggtga ccgatacctc ggacgaatca atcgcgcaat
      541 ggcgcgagca agccagccaa acgctcgcct cgttcggtat cgccggtgag cgtcctgtcc
      601 tcgcctacga tgccggcacg ctcttcgccg ctcggctctg gtgggtcctg cactatctgg
      661 gacatgagga taaacaggtc cttaacggcg gactatcggc atggcagcag gcaactggcg
      721 aggtcgccac cgacgccaca ccagccggag caacggcgag ctcggcggtt cagtccttcc
      781 agggacagcc gcgagccggg acactggcgc agctagagga agtacgggcg agtcttgacg
      841 acccgaacgt tgtgatcgtc gatgcccgaa cgcctgatga gtactccgct ggccacattc
      901 ccggcgccgt gaacatcaat tacccacgga atgcgacatc cgagatgccc aaggtctgga
      961 agccagtcga cgagttaaga gaaatgtacg aggaagtcgg cgtcactccc gacaagcacg
     1021 tcatccccta ctgctccacc ggcgtgcgat cagccgtcac gtttttcacg ctgagactga
     1081 ttggatacga gaacgtcgcg ctttacaccg gatcgtggaa ggaatggggc gaccatcccg
     1141 acacgccgaa gccaaccgga accgaaccgt aaaccatcgg cgctcgaagt ttaggaacac
     1201 ttttgcagcc acaaggagca agaatgaaac gagtcatcgt cttcgacgtc aatgagaccc
     1261 tgctcgatgt gggcgcgctc gatcccatct tcgcccggat gttcaacgac gcgtctgtca
     1321 ggcgcgagtg gttcgggcag atgcttcaat ccgcatttgt ctccaccatc actggagcat
     1381 atcgggattt cggggcaatc ggaataagcg ccctgaaaat ggtagcagag cgacacggag
     1441 tctcgcctgg tgacgaggac cttgccgctt tgcgcgacgg gatgcgtcag ttaccgccgc
     1501 atcccgaggt cagggaggcg ctagagcatc tccactcatc cgggtttcgc ctggcgaccc
     1561 tgacaaactc aacccttgag gtaagcgagg cacagctggt caacgctggc ctccacgaac
     1621 tcatggaaca gatgctctcc gctgacagtg tccatcgcct gaagccagca ccggagccct
     1681 accgaatggc ggccgagcgg cttggcgtca ctatcggcga gataagactc gttgctgcac
     1741 attcctggga tatcgccgga gctctgcatg cgggatgtgc cggggcattc gtcgcgcggc
     1801 cgggtcaggt cctcgacccg gacggtgctc agccggatgt tatcgggtcc gacatacggg
     1861 agattgcgga acgcatcctg gcgtccgatc aataagaagc tctcacatcc cagtcatcat
     1921 gaggtcggaa aatgaccgtt tcgtgcctgc atccctccag cggacctcct tgaccctact
     1981 ctatggagat cgtgtccgcc tggggcacgc ggtgtgcaaa gagggggcaa gcagcacaac
     2041 cgggatactt tccactgtga cgtgcgctca agacggtgta cgtgtgggag aaggcatccg
     2101 tggatgctcg tgttggctcg tggcggctgg tggttagaga gagtttctgg tttatcccgg
     2161 caatcaccac gctgctcgtg gtggtccttg cggtagtgct ggtgcggttc gacaggacgc
     2221 ttcgattcga tcagcggtcg gatctcccct ggtggctctt cggaggcggt cccgaaggta
     2281 tccgggcagt cctctccgcg atcgcgggca cgacgatcac cgttaccggt gtggtctttt
     2341 caatcacgat cgtcgccctc cagctcgcct caagtcagtt ctcacccctt gtgctcagga
     2401 ccttccgtgc cgacaggggc aaccagatag tcctcgggtt cttcatcgcc acgtttacct
     2461 attccctcct tgtgctacgg tcggtgctgt cccccgagca gggtactgag ggattcgccc
     2521 ctgtcatcac gtcgaccgga gccattgccc tggcactcgt gagcgtcacg ctactgatct
     2581 acttcattca tcacgctgct cgttccatcc aggcttccac gatcatagaa cgggcgtcca
     2641 atgacactct tgccctgata caccacctct tcccagagga tatcggggag cccggcgaat
     2701 cctacccttc ctccgcgctg ccgcaagcat tggcgaccgt tgttgtggcg aagcaaggag
     2761 gctatttcca ggcagtcgac ggcgacactc tcttcaacct ctccgaggaa caaacgctga
     2821 ccgtgcacgt cgagcggatc ccgggcgact atgtgctccc cgaatcggtc ctggccacgg
     2881 tctggccaaa gacgagcgtg gaccagggaa tggaggagaa ggtccgctcc gcatttctca
     2941 taggcccaga acgcacgctt cagtcagatg tcgagctggg aatcaggcaa atcgctgata
     3001 tcgcaataaa ggccttgtca ccggggatca atgatccgac gacagccatg ctctgcatcg
     3061 atcgcctgag cgaggcattg gttc
//
LOCUS       DASYWM010000138        16519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_661622, whole genome shotgun sequence.
ACCESSION   DASYWM010000138 DASYWM010000000
VERSION     DASYWM010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 16519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16519
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661622"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VGR29_05615"
     CDS             complement(<1..913)
                     /locus_tag="VGR29_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073103.1"
                     /translation="MPSSDQPITPPTTGIPTPPGGAGAADLATDSTDPQILRELLDRA
                     RERLAFYESFDRIIGENIRRSGELMVETVALREQAQALAAQSAMERATFAATRQTERD
                     RYRALVEQVLNEVSTVRPLIDTMVTRFESVLEELSEDTVTETGAGGVLDSTSISTNSA
                     LEAPSPESSETDEPPPVLVEDETPVTHVDPPAEEPAQETTEITTVEDAPPPDVAPEIT
                     PEEPAPEGSRGIDLLAHGVPNARVAISLQKMLRELDVVSSVEAREFANGELRLAIQAS
                     DALPEAALVAWLGDNNGELTSKSSSVTE"
     gene            1238..4117
                     /gene="lon"
                     /locus_tag="VGR29_05620"
     CDS             1238..4117
                     /gene="lon"
                     /locus_tag="VGR29_05620"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV2073104.1"
                     /translation="MPSRKSADDQAQANEPNETMDHEDQLAAEAPAPKRSRARTVKDV
                     TEELAAAITAEKARPREDSLDDEVPAKSKRTRSRRKKVSGDPDQLLLPVIITEETLLL
                     PHMSIPFPLVDEDTTMAVERAMRMDPRHVMLLTERRIPRKLDDGELQVDAATRDLIDI
                     MTDMIAQGDDEGARKLADEQGDFDIEAGLAGMPDDADYELCEWGVIAEVGQYITRPGG
                     QNHVILNGLARGRVEDIIQDQPFVAAKVRRVHEVVHPSAETEAAMGAMLDQVESYIAM
                     LPNVPEEVLEMVKSVDEPGWLADLIAFSPEFSSDQRQEMVETIDPLERLRKISVLVQK
                     RLNVLNLRHEIQSEAQAGMDKQQREYFLREQLRAIQKELGEGSSEEALANEIREKIET
                     AGMPDDVKQKALVQVERLEQQHPFSPEVGVIRTYLEWLTDLPWSHVTEDRLDLEEAAN
                     ILDEDHYGLEKVKDRIVEFIAVRKLAGDRLRAPILCFVGPPGVGKTSLGKSIARAIGR
                     EYVRMSLGGVRDEAEIRGHRRTYVGAMPGRVIKALKDAGTKNPVLVLDEVDKLGSDTF
                     RGDPSSALLEVLDPEQNTSFSDHYLEVPFDLSKVIFITTANMLDPIPPALRDRMEIIE
                     VSGYTELEKLEIARNFLLPKSLESHGLTDEQLIVNDGALKQIIREYTEESGVRNLERE
                     IGTLCRKIARTFAEANPPERVEVDGGDVHDYLGIPRYDFGLAEEQDEVGVATGAAVTS
                     VGGDLLSIEVTIMEGKGDLILTGQLGDVMQESARAAMSYARTRAATFKIEPGFFDKHN
                     IHVHIPAGAIPKDGPSAGITMATALISALTGRKVRKDVAMTGEMTLRGKVLPIGGLRE
                     KTLAAHRGGIKTFLLPKRNAKDLSELPEIVRKDLELIEVSHLDEVLEIALAKTPSKRR
                     RKASSGTPKVSDEPSTDDTPQRRDRRSPRIDAPGGKDPVPASVGT"
     gene            4349..5893
                     /locus_tag="VGR29_05625"
     CDS             4349..5893
                     /locus_tag="VGR29_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2073105.1"
                     /translation="MIRAAGGATLGAVVLVSRTPSMAENSQASPVPIMPGADWPLALG
                     NPARTGLAVDPGPEGEPELIWRFETGLSGTMASPVIVDGTVLTMGGLLGSAALVGLSL
                     QRGDMLWSLPMEFQRFGSAPAVADGVVCLAGLQGEVAAFDLTTQEQLRSTTLDARTTS
                     LPLVADGLLYIGANDDHLYALDIADGSERWRFPVVAGSHYTFGPSPAYADGSVYFPSA
                     SSKSDKGLYAVDALTGEEVWHFEPDTPGLFTPAIADGVAYLGGDGGDVYAVDTGSGER
                     RWKTTVSSAWSAPAVTGDLVFIQTMDGVLTCLDRESGELQWQVETAASWCAPIIAGEL
                     AYVGSGGLWFEMGLYAFDGATGEQRWHVRVDGVTAPVAVSGNVIVVGTDEGAVCAISG
                     SRADAVSRGTDREGTFLTSIIFSTEVDESNMPIDPTSAFPAQTSQLKASFSFVGMQYE
                     AAWEAIWMLDGEPILTRNESWAQGSSGRLAESIFANEGNLPVGRYELELRVNGTPIRR
                     GTAVIG"
     gene            complement(5900..6526)
                     /locus_tag="VGR29_05630"
     CDS             complement(5900..6526)
                     /locus_tag="VGR29_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV2073106.1"
                     /translation="MTTRDPATRDARDITPDEALAMLIEGNARYVDGHVTHMDYSAAR
                     TAAANPQVPVAGILGCGDARIGAELIFDRGPGDLFMVRLAGNFLSDYGLASMEYCVEF
                     LKVPLLMVLGHTHCGAVTAAIQVVQNKVDLPGRLFVLIDAIEPSVLHAQMSDPEDLLQ
                     AATVENVRRQVRRLRTISPVINAAQEAGHVKIVGAIYDMDTGKVNLLD"
     gene            6848..7471
                     /locus_tag="VGR29_05635"
     CDS             6848..7471
                     /locus_tag="VGR29_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073107.1"
                     /translation="MSKMFRLLDASMIAVLGFLGVMLSAGAQGGGTGTVIVLKYYCTY
                     LDETALVEAIEIDQCSPGAATFTFYLVGDGTNDFEQLNVSGAGRGSIELPVGEYEVVE
                     EGSQTHFMVNVLAGTNVQLLIANPSIEVVPTDPAPAPTAVPQPTTVPVEPTVVTAQPS
                     TTRTQPTRLPNTGSGSDVEPGVVAFALTAAALVAGSGAMILGKTRRD"
     gene            complement(7551..8816)
                     /locus_tag="VGR29_05640"
     CDS             complement(7551..8816)
                     /locus_tag="VGR29_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073108.1"
                     /translation="MDPASDQARIDLQKRIVRVLALAQICGGIGVAAGAAVGSLLAAD
                     LASDAFAGVSAASSVIGAAVIAIPVTRLMSDVGRRPGLILAYAIGIIGALLVVTGAAA
                     GVFPVALIGMLLAGGGTTATLQSRYAATDLAQGSRRGRDLATVVWATTIGSVLGPNLA
                     DPMGDVAEWIALPRLSGPYLLTIAIYLLAMVLIFTLLRPDPLLVAREDEEAHPDYIPG
                     TRLPRMSMKAAFALIRTSPPASVGLASMVLGHVVMVAVMSMTPVHLQHSDQTLKIIGL
                     VISGHITGMYIASPLVGNAVDRIGRRPVILAGAGILLASFVISGMASGHQSGQLMIGL
                     FLLGIGWSCTMISGSTLLTESVPLQNRPGVQGTADLTMGIAGASAGLLSGVVVGFGSY
                     ALLNTIAASLIVLLLLSLARERAHAIVPA"
     gene            8909..9088
                     /locus_tag="VGR29_05645"
     CDS             8909..9088
                     /locus_tag="VGR29_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073109.1"
                     /translation="MTDVGSSFRSDVCGWHLPLNQRFQFGDLRIEPAYTPVVVDPESA
                     GGAVNAFEIGEGSEA"
     gene            complement(9110..9763)
                     /locus_tag="VGR29_05650"
     CDS             complement(9110..9763)
                     /locus_tag="VGR29_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073110.1"
                     /translation="MHRLLAFPVLACQEVGIDPTLLPALHATNASFTESRGYQKVRIH
                     PQLVASGEQRGAYRIGAHQPPSMETPPQVWFKYLADMKAIQGNVNNDISFVSCLLAKD
                     YLRQRFEIPWFDAALKSQSANGLDIDERSIAGERIIGEIKTTTPHYAVKFGANQIVSL
                     KKGFQKLKKHQAEHKFMFVTDDRAFSVIAHSFCSDLGGVKLVCLTSGDEHYCDSHPV"
     gene            complement(9891..10286)
                     /locus_tag="VGR29_05655"
     CDS             complement(9891..10286)
                     /locus_tag="VGR29_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV2073111.1"
                     /translation="MTIIPDSYRDLLERPIVVSLATVMPDGQPQVTPVWAELLDGKIR
                     INTVVGRQKERNLSERKHATVLVIDPDNPLRFLEIRGEAAPSGEDDGIAVIDKLSKDY
                     LNIEPYPWRNPADTRITFLITPLHVVASG"
     gene            10404..10985
                     /locus_tag="VGR29_05660"
     CDS             10404..10985
                     /locus_tag="VGR29_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073112.1"
                     /translation="MVNRRRFVLIMALVVSLGATEHATAQRPDPHRATGDQPQATAVA
                     TPVSAGCELVESYTNDLYTAIDESDAFADFFYSDADFGDLTAAEAEEIVDDGTAMITA
                     LEDLDVPSAYADAHGGIVTFLQVNIDIARFYGIDSSVVPDLNAYDLATDAIYQGEIAL
                     AAACPPEIDAVGGYILLDPAGLDEEVIPENVPE"
     gene            11068..11811
                     /locus_tag="VGR29_05665"
     CDS             11068..11811
                     /locus_tag="VGR29_05665"
                     /inference="COORDINATES: protein motif:HMM:NF021617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEV2073113.1"
                     /translation="MGDELVAAREQARKHAEDVRGFVQHAGAFIAVGLFFLVVNLITG
                     PDSLWFYWPMLPWSIGFLIHGWNVFWDKRFFDEEWQERQVNKTLARMRTGEPLSSGPM
                     AATEIQEILDRASGLVDGMRSSARRIPDPDVRHKALDACAASDQVLAAIIDHPDELQL
                     ARDFVNRFLEPANAVMSDYSRLATRNVPSARETLSKVEQHDLPLITHKANQLYDRLHR
                     GTLIDLEVAREMLTLDMANTSVPELPAKD"
     gene            complement(11883..13499)
                     /gene="murJ"
                     /locus_tag="VGR29_05670"
     CDS             complement(11883..13499)
                     /gene="murJ"
                     /locus_tag="VGR29_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEV2073114.1"
                     /translation="MTPRFWRRPQPSILRSGSARSLAGNAAIVAAAFAASRVLGLVRE
                     VVIASRFGTGHTYDAYVAAFRIPDLIFFIVMSGAFGSAFIPVFGGFLAKGDAERAWNL
                     ANTLLTWVVAGFAVVALLVSLLAKPLIGVVIAPELPAESQDLAVNLTRLLLLSPLLLG
                     LSAAGKGMLEAQSAFILPAIAPILYNLGIIGGALLLTPVMGVYGLAAGVILGSLMHVA
                     LQFGSLLRNDLQLHPSFSPRTDGLAEVGRLMAPRILGQTVSQVNLIVMTNFASRLAEG
                     SISALNYAQSLILLPHGILAMSLSTVIFPRMVQDHERGDKKAFRRTLSVGLRTLIFLT
                     LPAAVGLLVYRTSIVQMLFQYGSFNEESTDMVATAVGFFSIGLIGRAVAEPVTRTFYA
                     MRDTRTPVVVSILSVVVNIALSSALASSMGHAGLALSLSATYLLRMVILIIILSRRAG
                     GLISELAPPVTRMLVPLAVFTAVALVAATPLARATNPADGRTLLAYVLFGLVLVCSMG
                     LYLVTSWLLRLPEAIRLANTAKRRFRGFRD"
     gene            complement(13715..14941)
                     /locus_tag="VGR29_05675"
     CDS             complement(13715..14941)
                     /locus_tag="VGR29_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073115.1"
                     /translation="MNSGSPKQRHGPTAFVLALGFPGLGAIRSLGRAGVPVVGLDPTP
                     GGLGFASRYCDARRCPNPVTEPDLLVEYLLDAQRKLDEPGILSPASDAFVLFISRHRK
                     LLGEHFRFNLPSPEVIESAVDKRKLYETAERVGIAYPETFYPEMMDDVHRIKHEITYP
                     VYVKPYHSHLWHAAFPKSGKGIKAFTPGQLVSAYERVFAAGVEAMVQSIILGPASNIR
                     TARVYMSEQGELLALLTTRTIRQWPVEFGVGTMLESWRNRELAEMGTRFFRDIDFRGV
                     GTIEFKRDERDGVLKVTDLNPRWWGSIQLAPSSGVDFPLIHYLDLVGQSPDPQLTFKE
                     GVRWIDGRGDFASAMTLVRSRQLKVREWLRDCTGVRSFSTFALDDLGPFLKKYNYGRS
                     LFEAPIHVRSKKRRQH"
     gene            15536..16273
                     /locus_tag="VGR29_05680"
     CDS             15536..16273
                     /locus_tag="VGR29_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEV2073116.1"
                     /translation="MDLARMEFFSRALGKRASYNVLLPETGEGPFPVLFQLHGLTDDC
                     NTWIQRSNIALHANAYPMVIVFPDGGTSAYLNWKAAGRLGRQNYEDLIIIDISDHVRR
                     HLHVTDGPWAIGGFSMGGWGAMWLGLKYPDRFASIWSHSSKFNWRDSDLDFSMLAQPE
                     DVDLPAYAERISMLEQKPIISFDCGVDDQLIEENRDFHNHLEKIGLEHHYSEHPGGHT
                     WVYWDDHVREALAQHARVLGLTMSGSA"
BASE COUNT         3374 a         4693 c         5304 g         3148 t
ORIGIN      
        1 gctcagtcac tgaggatgat ttgctggtca actcaccgtt attgtcgcca agccaggcaa
       61 cgagggcggc ttccgggagg gcatcgctag cctgtatcgc caggcgaagc tccccgttgg
      121 caaactcccg tgcctccacg ctgctgacga catccaactc acggagcatc ttctgcagcg
      181 agatagcgac cctggcattc gggacgccat gggcgagcag gtcaatgcct cgggagccct
      241 ccggggccgg ctcctcgggt gttatctctg gggccacatc ggggggagga gcgtcctcga
      301 cggtggtgat ctccgttgtt tcctgggcgg gctcctcggc tggggggtcg acgtgggtga
      361 ctggcgtctc gtcctcaacg aggacgggtg gcggctcatc tgtctcgctg gattcgggag
      421 acggtgcctc aagggcggag ttggtggaga tgctggttga gtcgaggaca ccgcccgcgc
      481 cagtttccgt gacagtgtcc tcgctcagct cctctaggac gctctcaaac cgcgtcacca
      541 tcgtgtcgat gaggggccgc accgtggaaa cctcgttgag gacctgttcg acgagcgcac
      601 ggtagcgatc acgctcagtt tggcgcgtgg ccgcaaacgt ggcgcgttcc attgccgatt
      661 gcgccgcaag tgcctgtgcc tgttcacgga gggcaaccgt ctccaccata agctcaccag
      721 accgacggat gttttccccg atgatccgat cgaatgactc gtaaaacgcc agtcgttccc
      781 tcgcgcgatc gaggagctca cgcagtattt ggggatcggt cgaatcggtc gccagatcgg
      841 cggcgccagc tccgccaggc ggcgtgggga ttcccgtcgt tggcggggtt atgggctgat
      901 cggacgaagg catctgggct ccgtacatat gacgcggcat caaatctcta caaggggcag
      961 tgtagcaaac gagcggccgg tgccttctaa ggtggacacc gcggtataca attcaatgca
     1021 gagaatgcgg attgtcgtgc acgggattgc acccagatcg tgagcccgcc cgcactgagg
     1081 gcagaccggg ccagagcgtc cttacccccg taccagacgt ccattgggcc gcgtgagcgg
     1141 ccagcgttgt caaacaatct ccccaggccg gaacctcctg gtgcgtgtgc acggtgtgca
     1201 cgtgtttttc actctggaaa gtcgagcaag agcaaccatg ccgtcgcgaa aatccgctga
     1261 cgatcaggcc caagccaacg aaccaaacga gactatggat cacgaagacc agctggcggc
     1321 tgaagcaccg gcgccgaagc gctcacgggc gcgtaccgtc aaggacgtaa ccgaggagct
     1381 tgccgccgcg atcaccgccg agaaggcgcg cccgcgggaa gattccctgg atgacgaggt
     1441 gcctgccaag tcgaagcgca cccggagtcg ccgaaagaaa gtttccggtg atccggatca
     1501 gttgctgctc ccggtgatca tcaccgagga gacactactg ctgccccata tgtcgatccc
     1561 gttcccgctc gtggatgagg acaccacgat ggccgtcgag cgggcgatgc gtatggatcc
     1621 tcgtcacgtc atgttgctga cggagcgacg gatcccccgt aaacttgacg atggcgaact
     1681 gcaggtcgat gccgctaccc gtgacctaat cgacatcatg accgacatga ttgcccaggg
     1741 tgacgacgag ggcgcccgca agctcgccga tgagcagggc gatttcgaca tcgaggcggg
     1801 gcttgccgga atgccggatg acgccgatta cgagctctgc gaatggggcg tgatcgccga
     1861 ggttgggcag tacattaccc gtcccggtgg ccagaaccat gtgattctca acggcctcgc
     1921 acggggacgg gtagaggaca tcatccagga ccagcctttt gtcgcggcca aggttcgccg
     1981 cgtgcatgag gtggttcatc cgtcagccga aaccgaagcg gcaatggggg cgatgctgga
     2041 tcaggtcgag agctacatcg ccatgctgcc gaatgtcccg gaggaagtgc tggagatggt
     2101 gaagagcgtc gatgagccag gatggctcgc cgacctcatt gccttctcac cggagttctc
     2161 ctccgaccag cggcaggaga tggtggagac aatcgatccg ctcgagcgcc tgcgcaagat
     2221 cagcgtgctc gtccagaagc gcctcaatgt gctcaatctg cgacatgaga tccagtccga
     2281 agcgcaagcc ggcatggata agcagcagcg cgagtacttc ctccgggagc agctccgggc
     2341 gatccagaag gagctgggtg agggctcgtc ggaagaggcc ctggcgaacg agattcgcga
     2401 gaagatcgag accgccggga tgccggacga tgtcaagcag aaggcgttgg tccaagtcga
     2461 gcgcctggag cagcagcatc ccttctcgcc agaggtcggt gtcatccgaa cctatctgga
     2521 gtggctaacc gatctgccgt ggtcgcacgt gacagaggat cgactcgacc tggaagaggc
     2581 ggcgaacatc ctcgacgagg atcactacgg gctggagaag gtaaaggatc gcattgtcga
     2641 gttcatcgcg gtgcgcaagc ttgcgggcga caggctgcgg gcgccgatcc tctgcttcgt
     2701 tggccctccg ggcgtcggca agaccagcct tggcaagtcc atcgcccgcg ccatcggacg
     2761 cgaatacgtc cgtatgtccc tcggcggggt gcgggacgag gccgagatcc gcgggcatcg
     2821 gcgcacctac gtcggggcca tgccgggtcg cgtgatcaag gcgctcaaag atgccgggac
     2881 caagaacccc gtgctcgtgc tggacgaggt cgacaagctt gggtcggata ccttccgtgg
     2941 tgatccctcg tcggcgctcc tggaagtgct ggatccggaa cagaacacat cgttttcgga
     3001 tcactacctg gaggtgccgt tcgatctctc caaggtgatc ttcatcacga cggcgaacat
     3061 gctcgatccg atcccgcccg cattgcggga ccgcatggag atcattgagg tgtccggcta
     3121 caccgagctg gagaagctgg agattgcgcg gaatttcctg ttgcccaagt cactggagtc
     3181 gcacggtctc accgacgagc aactgatcgt caacgatggc gcgttgaagc agatcattcg
     3241 cgagtacacg gaggaatcag gcgtccgaaa cctcgagcga gagatcggaa cgctctgccg
     3301 caagattgcg cggacgtttg ccgaagccaa cccaccggag agagtggagg ttgatggcgg
     3361 tgatgtccac gactatctcg gcataccgcg ttatgacttc gggcttgccg aggagcagga
     3421 cgaggttggt gtcgcgacag gcgctgcagt taccagcgtt ggcggcgact tgctgtccat
     3481 tgaagtcaca attatggaag gcaagggcga cctgatcctt accggccagc ttggtgacgt
     3541 gatgcaggag tcggcgcggg cggcgatgtc ctatgcgcgg acgcgagcag cgacattcaa
     3601 gatcgagcct ggatttttcg acaagcacaa tatccatgtc cacatcccgg cgggtgcgat
     3661 tcccaaggac ggcccatcgg ctggcatcac catggcgaca gccctgatct ctgccctgac
     3721 cggacgcaag gtccgcaagg atgtcgcgat gacgggagag atgacgttgc ggggcaaggt
     3781 cctgccgatc ggtggcctgc gcgagaagac gctggcggcg catcggggcg ggatcaagac
     3841 ctttttgctg cccaagcgga acgccaagga tctctcggag ctccccgaga tcgtccgcaa
     3901 ggacctggag ctgatcgagg tctcgcacct ggacgaggtg ctggagatcg ccctggcgaa
     3961 gacgccatcc aagcggcgcc gcaaggctag ctctggcaca ccgaaggtct ctgacgagcc
     4021 ctccaccgac gacacgccgc agcggcggga ccggcgctca ccgcgcattg acgcgccggg
     4081 cggcaaggat ccggtaccgg cgtcagtggg aacgtaatcg accggtcgcc aataccagtg
     4141 acaggaaaca gggcagcgat catccgctgt cctgttttca ttgccgaggc tttccctttt
     4201 gggtggcctg gcgggaacat agggcgctga tgtgtcgtct ttttatcagg gtgatcccat
     4261 cttgggagcg atctccgagg tagcatggga gcgagtgaac catgcatgat gcgccggggg
     4321 gtggaacggg gcgatcgacc ggagaacggt gattcgagcg gctggaggcg cgacgctcgg
     4381 tgcagtggtg ctggtgtcca gaacgccgtc aatggcggag aactcccagg cttcgcccgt
     4441 gcccatcatg ccgggcgccg attggcccct ggcgctggga aatccggcga ggaccggcct
     4501 ggccgtggac ccgggaccgg agggtgagcc ggaactcatc tggcgcttcg agacggggct
     4561 gtcgggaacc atggcatcgc cagtgatcgt tgacggaaca gtgctcacca tgggtgggct
     4621 gctgggcagc gccgcgcttg tcgggctcag cctgcaaagg ggcgacatgc tatggtcgtt
     4681 gccaatggaa tttcagcgct ttggctccgc acccgcggtc gccgatggcg tcgtgtgtct
     4741 ggcgggtctg cagggagagg tagccgcatt cgacctgacg acccaggagc agcttaggtc
     4801 gacgacgctg gatgccagga ccacctcctt gcccctcgtg gcggatggac tgctgtacat
     4861 tggcgccaac gatgatcatc tctacgcgct cgatatcgca gacggtagcg agcggtggcg
     4921 attcccggtc gtcgcaggat cccattacac ctttgggcca tcaccggcct acgccgacgg
     4981 ctcggtctat tttcccagcg ccagctcaaa aagcgacaaa ggactatacg ccgtggatgc
     5041 cctcaccggg gaagaggtct ggcacttcga gccggataca ccaggtttgt tcaccccggc
     5101 tatcgcagac ggcgtggcct acctcggcgg agatggtggc gatgtctacg cggttgatac
     5161 cgggagcggt gagcggagat ggaagaccac cgtgtcgagt gcctggtccg ctccggcagt
     5221 taccggcgat ttggtgttca tccaaacaat ggatggagtg ctgacctgtc tcgatcgcga
     5281 gagtggggag ctccagtggc aggtggagac agcggcctct tggtgcgctc ccatcattgc
     5341 cggggagctt gcatatgtcg gttccggcgg actttggttt gagatgggtc tctatgcgtt
     5401 cgacggggca acgggtgaac agcggtggca cgtgcgcgtt gacggtgtca ccgcgccagt
     5461 ggcggtctcc ggcaacgtga tcgtggtggg caccgacgaa ggcgctgtgt gcgccatcag
     5521 cggatcaagg gccgatgctg tctcccgtgg cacggacaga gaggggacct tcctcacgag
     5581 catcatcttc tcgacggaag tcgatgaaag caatatgccg atcgacccga cgagtgcctt
     5641 tcctgcccag acaagccaat tgaaggccag tttcagcttt gtggggatgc agtacgaagc
     5701 ggcctgggag gctatttgga tgctggatgg cgagcccatc ttgacgcgca acgagtcctg
     5761 ggcgcaggga tcttcgggac gtttggcgga gagtattttc gccaatgagg gaaacctccc
     5821 cgtaggccgg tacgagctcg agttgcgtgt gaacggtacc cctatccggc gtggaacggc
     5881 ggtcatcggc tgagtctgtt tagtccagca agttcacctt cccggtatcc atgtcgtaga
     5941 tggcgcccac gatcttgacg tggccggcct cctgcgcggc attgatgacc ggggagattg
     6001 tgcgaagacg ccgaacctgc cggcggacgt tttccacggt cgctgcctgg agcaggtcct
     6061 ctggatcgga catctgcgcg tggaggacag agggctcgat cgcgtcgatc aggacgaaga
     6121 ggcgtcccgg gaggtccacc ttgttctgga cgacctgaat tgcggcggtg accgcaccac
     6181 aatgggtgtg cccgagcacc atcaggagtg ggaccttcag gaactcgacg cagtattcca
     6241 tgctggccag gccgtaatcg ctcaggaagt tcccggccaa gcgcaccatg aagagatcgc
     6301 ccggacctcg gtcgaagatc aactcggcgc caatacgagc gtcaccgcac ccgaggattc
     6361 cggcaacagg tacctgtgga ttagctgcag cggtgcgtgc cgctgagtag tccatgtgcg
     6421 tgacatgccc gtccacatat cgtgcattcc cctcgatgag catcgcaagc gcctcgtctg
     6481 gcgtaatgtc gcgagcgtca cgtgtggccg ggtcccgggt ggtcatctac tttctctcct
     6541 atcgtatgcg cagggattga gaaactttgc gagaagcgac ctggcacatt cctgcgtcgc
     6601 cgttcccccc ttgtgtctcg cccagatgtc atgcggaacc ggatatgcac atggtgtgag
     6661 tatggtggga gcctgccaag tcaatcgctc aagaaacgct tcttgctcgc caatcgttgt
     6721 gatgattagc gtcatgagcg tcatggcacg ggtcccttca tcataaagca gcagggcgaa
     6781 caatcctctt cgcgatgcac tggcgtcatc cgcggtgctt gacgtccctg caggaggtag
     6841 acacgtcatg tcgaaaatgt ttcggttact tgacgcgtcg atgatcgcgg ttctcggttt
     6901 tctcggggtc atgctcagtg ccggcgcaca gggaggtggt accggcacgg tcattgttct
     6961 caagtactac tgcacttatc ttgacgaaac cgcgcttgtc gaagccatcg agatcgatca
     7021 atgttctccg ggcgcggcga cgtttacctt ctatctcgta ggcgacggta cgaacgactt
     7081 cgagcagtta aacgtcagtg gtgctggccg ggggagcatc gagcttccag tcggcgagta
     7141 tgaggtagta gaagaaggat cgcaaaccca cttcatggtc aacgtgttgg ccgggacaaa
     7201 cgtccagctg ctcattgcca atccatcgat tgaggttgtc ccaaccgatc ctgcaccagc
     7261 cccaaccgcg gtccctcagc ccacaaccgt acccgtagag cccacggtcg tgactgcaca
     7321 gccgtccacc acgcgaacgc aaccgacccg gctgcccaat acggggtccg gttcggatgt
     7381 ggagccggga gtggtggcat ttgctctcac tgccgcagca ttggtcgccg gaagcggcgc
     7441 aatgatcctg ggcaagacgc gcagggactg attgctgaca tcggattgga gagcggagag
     7501 cgggggccat tgccaggtgg ctcccgcttt cgttgtctgc cgcaacggat tcaggctggg
     7561 acgatcgcat gcgcccgttc ccgggccaat gagagaagaa gaagcacgat gagggaggcg
     7621 gcgattgtgt tcaggagcgc gtaggagccg aacccgacaa ccacgccgga gagcaggccg
     7681 gcgcttgcac cggcaatacc catcgtcagg tctgccgttc cctggactcc ggggcgattc
     7741 tggagcggca cggattccgt gagcagcgtc gagcccgaga tcatggtgca cgaccagcca
     7801 atgccgagga ggaagagacc gatcatcagt tgtcccgact ggtgcccgct tgccatgccc
     7861 gagatcacaa aggaggccag cagaatcccg gccccggcaa ggatcaccgg acggcgaccg
     7921 atcctgtcga cggcattgcc aaccagtggt gaggcgatgt acatcccggt gatatgcccg
     7981 ctaatgacga ggccaatgat cttgagcgtc tgatcactgt gctggaggtg tacgggggtc
     8041 atcgacatga cggccaccat cacaacgtgt ccgagaacca tagatgcgag cccaactgac
     8101 gcaggagggc tcgtccggat gagcgcgaag gcagccttca tcgacattct cgggagtcgg
     8161 gttcccggga tgtaatcggg atgagcctcc tcatcctcgc gggcaacaag aagcggatcc
     8221 gggcgtagca atgtgaagat cagcaccatc gccaggagat agatcgcgat agtgagcaga
     8281 tagggaccgg agaggcgagg gagggcaatc cattcggcaa catcgcccat cgggtcggcg
     8341 aggtttgggc cgagcaccga gccgattgtg gtggcccaga cgacggtggc cagatcgcgg
     8401 cctcgtcggg agccctgggc gaggtcggtc gccgcatagc gggattggag ggtggcggtg
     8461 gtgccgccgc ccgcgagcag catgccgata agggcgaccg ggaaaacccc ggctgcggct
     8521 ccagtgacga cgagcaaggc gccgatgatg ccgattgcat acgcgaggat caggcccggc
     8581 cgcctgccga catcgctcat caggcgagtg acagggatgg cgatgacggc ggcaccgatc
     8641 accgacgagg ccgcagagac accagcgaag gcgtccgatg cgaggtcggc ggcaaggagt
     8701 gaaccgacgg cggcccccgc tgcgacgccg atgccgccgc agatttgagc cagcgcaagc
     8761 acccggacaa tgcgcttttg aagatcgatc cgcgcctggt cgctggctgg gtccacgcat
     8821 actccataca gagagaggcc gccggggcga tgacccaagc ggcgcgtatt catcgtgccg
     8881 tgattgtcgc acggagtgga gaagcacggt gactgacgta ggctcatcct tcaggtccga
     8941 cgtttgcggc tggcatttgc cgctgaacca acgctttcag ttcggggatc tgcgaattga
     9001 gccggcctac actccagtgg ttgtcgatcc ggagagtgcc ggtggtgcag tcaatgcctt
     9061 cgagattggc gaaggatctg aggcataaaa ggctatgcct ccgcaggaat cagactggat
     9121 gggaatcgca gtagtgctca tctcccgagg tcaggcacac cagtttcacc cctcccaaat
     9181 cggaacaaaa gctgtgcgct atgacgctga aggccctatc gtccgtgacg aacataaact
     9241 tgtgttccgc ctggtgtttc ttgagcttct gaaagccctt cttgagcgac actatctggt
     9301 tcgcaccgaa tttgactgca taatgaggcg ttgtagtctt gatctctcca atgatcctct
     9361 caccagcaat cgagcgctcg tcgatatcca agccattcgc actctgagac ttcaatgcgg
     9421 cgtcaaacca tgggatctca aatcgttgtc gcaaatagtc tttcgccagg agacaggaca
     9481 caaagctaat gtcgttgttg acgtttccct ggattgcctt catatcggcc agatacttga
     9541 accacacttg gggcggcgtt tccatgctgg gcggttgatg ggcgccaatt ctgtaagcac
     9601 cacgctgctc tccagatgcg accagttgag ggtgaattcg aactttctga tatccccgtg
     9661 attctgtgaa tgatgcgttg gtagcatgaa gggccgggag aagggttgga tcgatgccaa
     9721 cctcctgaca cgcgagcacg ggaaaggcca gaagccggtg caaggacggc acgctcctgg
     9781 gggaaagacg gggctggcta accgccacca cgtcaattca ctggtccgtc agccggcccc
     9841 aggaccagcg gcatgtgcag cgagagacag aaggcatggc cgttgacctg ctatccgctt
     9901 gcgacgacat ggagcggagt aatcaggaag gtgatccggg tatcagcggg gttgcgccag
     9961 ggatagggct cgatgttgag gtagtccttc gagagtttgt cgatcacggc aatgccatca
    10021 tcctcgccgg aaggtgcagc ctcgccacga atttccaaga agcgcaaggg gttgtccggg
    10081 tcgatgacca gcaccgtcgc gtgcttccgc tcactcaggt tgcgctcctt ctggcgccca
    10141 actaccgtat tgatccggat cttgccatcc aggagctcgg cccagactgg tgtgacctgc
    10201 ggttggccat caggcatgac cgtcgccagc gagacgacga tagggcgttc cagcagatcg
    10261 cgataactgt caggaatgat cgtcacggaa gtccttagtg aggggttggt ctcatgtgct
    10321 gcgccctaac gtggtgacgc tcggactatc ctatagtaga cagggatggg attgcatgag
    10381 agacgcagaa agaggtgatg cgcatggtga acaggcgccg gtttgtactg atcatggcat
    10441 tggtggtctc gctgggggcg acggagcatg ccactgcgca acgacccgac cctcatcggg
    10501 ctaccggtga tcagccgcag gcaacggcag tggcaacgcc ggtgtcagcg gggtgcgagc
    10561 tggtagagtc ctacaccaat gacctctata cagcgatcga cgaatcggac gcattcgctg
    10621 acttcttcta ttccgatgcc gatttcgggg atctcacggc ggcagaggcg gaagagattg
    10681 tcgacgacgg gaccgccatg atcacggcgc ttgaggatct cgatgtgccc tcggcctatg
    10741 ccgacgctca tggaggcatt gttacgttcc tgcaggtcaa tatcgatata gcgagatttt
    10801 atggcattga ttccagtgtc gtgccggatt tgaacgctta cgatctggcc accgatgcga
    10861 tctaccaggg tgagattgcc cttgcagcgg cctgtcctcc ggagatcgac gcggtcggtg
    10921 gctatatcct gcttgatccc gcgggcctgg atgaagaagt gattcccgag aatgtaccgg
    10981 agtgagggaa gggccggatg ccgagctcga tcaggatgac ggttgactgg ccggggcggt
    11041 gcaggtacca aacaggtgga gcggagcatg ggtgatgagc tggtagccgc cagggagcag
    11101 gcgcgaaaac acgcggagga tgtgcgaggg tttgttcagc atgccggcgc attcattgct
    11161 gtcgggctct tttttctcgt cgtgaacctg atcacggggc ctgacagctt gtggttttac
    11221 tggccgatgc ttccctggag catcgggttc ctcattcacg gctggaacgt gttttgggac
    11281 aagcggttct ttgatgagga gtggcaggag cggcaggtca acaagacgct tgcacggatg
    11341 cgtaccgggg agccactgtc atcagggccg atggcggcga cggagatcca ggagattttg
    11401 gaccgcgcat ctgggctggt ggatgggatg cggtcaagcg cccgccgtat ccctgatccg
    11461 gacgtccggc ataaagcact ggatgcctgt gcagcgtcgg atcaggtgct tgcggcgatc
    11521 atcgaccatc ccgacgagct gcaactggcg cgggacttcg tcaaccgctt tctcgagccg
    11581 gccaatgcgg tgatgagcga ctactcccgc ctcgcaaccc ggaatgtgcc ctctgcccgt
    11641 gagacacttt ccaaagtcga gcagcacgat ctgccgctga tcacccacaa ggccaaccag
    11701 ctttacgacc gcctccaccg gggaacgctc atcgatctcg aggtcgcgcg cgagatgctg
    11761 accttggaca tggcgaatac gtcggtaccg gagctgccag ccaaggactg agctgccgga
    11821 gcaaacgtga ggaggaaggg gcgaggcagc acaatcccac cttcgatgga ggtccataac
    11881 tctcaatcac ggaatcccct gaatcgacgt ttcgcggtat tggccagacg gatcgcctcc
    11941 ggcagcctca ggagccagga cgtgaccagg tagaggccca tgctgcagac gaggaccagg
    12001 ccaaagagca cgtatgcgag gagtgtccgt ccgtcggcgg gattcgttgc ccgtgccaga
    12061 ggagtcgcag caaccagggc tacggcggtg aagacggcaa gcggcaccag catccgggtt
    12121 acaggcgggg cgagctccga tatgagaccc ccggcgcgtc tggagaggat gatgatcagg
    12181 atgaccattc gaaggagata ggtggcagag agactgagag caagacctgc atgtcccatg
    12241 gaggatgcga gcgccgagct cagcgcgata ttgacaacga cactcaagat cgacacgacg
    12301 accggcgtcc gcgtgtcgcg catcgcatag aaggttcggg tcaccggctc ggcaacggct
    12361 cgtccaatga gtccgatgga gaagaagccg acagcagtgg ccaccatgtc cgtcgattcc
    12421 tcattgaacg aaccatactg aaagagcatc tggacaatgc ttgttcggta aaccagtagc
    12481 cccactgccg cgggcagcgt caggaagata agcgttcgca ggcccacgct cagtgtccgg
    12541 cggaacgctt tcttgtctcc gcgctcatgg tcctgaacca tccgtgggaa aattacggta
    12601 gacaggctca tggcgaggat gccatgaggt agcaggatga gggattgggc gtagttcagc
    12661 gcagagatac taccttcggc caggcgcgac gcaaagtttg tcatcacaat caggttcacc
    12721 tggctcacgg tttgacccaa aatgcgtggg gccatcagcc ggcctacttc ggcaagacca
    12781 tcggtgcgtg gtgagaatga tggatgaagc tggaggtcgt tgcgcaggag cgatccgaac
    12841 tgaagcgcaa cgtgcataag ggaaccgagg atgactccgg cggcaaggcc gtagacaccc
    12901 atcaccgggg tgaggaggag tgcgccaccg atgatcccga ggttgtagag aatcggggcg
    12961 atggccggga ggatgaaggc gctttgcgcc tccagcatgc ccttgccagc ggcactcagg
    13021 ccaaggagga gtggggagag caacagcagc cgtgtcaggt tcacggcaag gtcctgactt
    13081 tccgctggca actcgggggc gataaccacg ccgatcagtg gcttggccag cagggaaacc
    13141 agtagcgcga cgactgcgaa tcctgcaaca acccaagtca gcagggtgtt cgcgaggttc
    13201 cacgcacgtt cagcatcgcc cttcgcgagg aatccaccga agacggggat gaacgcagaa
    13261 ccgaaggcgc cactcatgac aatgaagaag atgagatcgg ggatgcggaa ggccgctacg
    13321 taggcgtcgt atgtgtggcc cgtgccgaag cgggacgcaa tcacgacttc gcggaccagg
    13381 ccgaggacgc gactggctgc gaatgctgcc gcgacgatcg ccgcatttcc tgcaagagag
    13441 cgtgcggaac cggatctcag tatggagggc tgcgggcggc gccagaaccg tggtgtcacg
    13501 tcgggattgt acctgaacca ggaccccgcg aaagggtgca ggtgattgtg tagtgacctg
    13561 tgaagagacg tggtctagtg tgcatgcaag cgattcagtc accgacttcc gtgtcatacc
    13621 agcgcctcta tgcgatgtcc ctcgtcagtt tcaaccaaag tggaaacata agggccggtg
    13681 ccggcttacg tccgaaaacc agaccgtggg ggcatcagtg ttgccgcctc ttcttgctcc
    13741 ggacatgaat cggcgcttca aacaatgacc ggccgtagtt gtactttttc aggaaagggc
    13801 cgaggtcgtc gagcgcgaag gtggagaacg agcggacgcc cgtgcaatcc cgtagccatt
    13861 cccgcacctt tagctggcgt gaccgtacga gtgtcatggc agaggcgaaa tcacctcggc
    13921 catcaatcca ccgtactcct tctttgaaag tgagttgtgg atccggtgat tgaccgacaa
    13981 gatcgagata gtgaatcaag gggaagtcaa cgccagaact gggtgccaat tgaatcgacc
    14041 cccaccaccg cgggttcagg tcggtcacct tcaggacgcc gtcccgttcg tcccgcttga
    14101 actcgatcgt gcccaccccc cggaagtcga tatcgcggaa gaaccgggtc cccatttctg
    14161 caagttcacg gttacgccag ctttcgagca ttgttcccac gccgaactcg accggccact
    14221 gccggattgt tcttgtcgtc agcaaggcaa ggagctcgcc ctgttccgac atgtagacac
    14281 gtgctgttcg tatgtttgag gctgggccaa ggatgatcga ctggaccatc gcctctacac
    14341 cagcggcgaa aacacgctcg tacgcggaca ccagttgtcc aggggtgaac gccttgatgc
    14401 ccttgccaga tttcgggaac gccgcatgcc agaggtgtga gtggtacggc ttcacgtata
    14461 ccgggtacgt tatctcgtgc ttgatgcggt gaacgtcatc catcatttcg ggataaaacg
    14521 tctccggata cgctatccca actcgctcag ccgtctcgta aagctttcgc ttgtcgacgg
    14581 ctgattcgat gacttcgggc gagggcaggt tgaaccgaaa gtgttcaccc aacagcttcc
    14641 ggtgacggga aatgaagagg acgaaggcat ccgaggctgg cgagaggatg cccggttcgt
    14701 cgagtttcct ctgtgcgtcc aaaaggtact caacgagtag atcgggctcg gtgacggggt
    14761 tcggacatcg tctggcatca cagtagcgcg atgcgaaccc gagaccacct ggagtggggt
    14821 cgagccccac caccggtaca ccggcgcgtc cgaggctgcg gatcgctcca agccccggga
    14881 atccgagtgc gaggacgaag gctgttggtc cgtgtctctg cttcggcgat ccgctgttca
    14941 ataggcgcat acactcccta accccgttgg ggggaaccaa ctgtcgcaag acattgtact
    15001 tagcgtcagg aatattgtgt gcgggatggc gtggaaaggc tgctcaccaa gggcaattcc
    15061 tggttcacag tcacgtgggt gctttgcacc acgccctccc gagtgcataa ggctgcgcgc
    15121 gatataggac aactcactgc ggtggtccca cagcaacatc gccatccgag tcttttgtgc
    15181 ctcagtatca cacactatgt ccagtaaacc gctgtgcccg gacgtgcgga ggaggataag
    15241 ccttatcgat aaccttggct ggcgtatttt gccgtcactg atttagttgt tggcgcatca
    15301 cctgccggta tggagttgcc agcacacact cataaggtca cgtgggtcat tactcacctt
    15361 gtcggggcca atcgtggcat tctcccggtg tattaatgtt ccgcgtgcaa tcacatgcgc
    15421 ttgtctctgc ccgtaggtgc gcggcgtggc gtttggatgc atgttgcgga tttgccatga
    15481 cagttcggca atcgtgacct gtgtagacca gacagggatg ggagaaggag gaggaatgga
    15541 tctcgcccga atggaattct tcagcagagc cctcggcaaa cgcgccagtt acaacgtgct
    15601 cctgccggag acgggggagg ggccgttccc cgtgctcttc cagttgcatg gcctgaccga
    15661 tgactgcaac acctggatcc agcgctccaa cattgccctg cacgccaacg cctatccgat
    15721 ggtgatcgtc ttcccggacg gcggcaccag cgcgtacctg aactggaagg ctgcgggtcg
    15781 gcttggcagg cagaactacg aggacctgat catcatcgat atcagcgatc atgtccgtcg
    15841 ccatctccac gtgaccgacg gcccatgggc gatcggtggg ttctcgatgg gtggttgggg
    15901 agcgatgtgg ctcggactca agtatccgga ccgctttgcc tcgatctggt ctcactcctc
    15961 gaagttcaac tggagggact cagatctcga tttctcgatg cttgcgcaac cagaggatgt
    16021 ggacctgccc gcgtatgccg aacggatctc gatgctggag cagaagccga ttatctcctt
    16081 cgattgcggc gtggacgacc agttaatcga ggaaaaccgc gacttccaca atcatttgga
    16141 gaagatcggg ctggagcatc actatagtga gcatccggga ggccatacct gggtctattg
    16201 ggacgaccat gtgcgcgaag cacttgccca acatgccagg gtgctcgggc tcactatgtc
    16261 ggggtcagcc tgatggcgag cgagatccgg gtgctggcag ccgctccgcg gccatcgtgc
    16321 acagtggtgg tgactgaggg aatgccgggc ggaacgaggt ccgcgactac atccatccaa
    16381 ggacaacggc gagaaatccg catccgtagg tgccacaagt gtggacacag gccccccaga
    16441 tcgcctggat ggtatccgcc acgccggaca tgctcaacga aggccagcct aaggtcctta
    16501 ggggcaacaa cgacctgat
//
LOCUS       DASYWM010000139         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_568211, whole genome shotgun sequence.
ACCESSION   DASYWM010000139 DASYWM010000000
VERSION     DASYWM010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568211"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VGR29_05685"
     CDS             complement(<1..232)
                     /locus_tag="VGR29_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEV2073117.1"
                     /translation="MELATEEDRYDIETREDIARLVETFYTRAFTDPVLGPIFIDVVQ
                     MDLAAHMPIMCDFWENILFGARKYPGGMMMVHF"
     gene            532..963
                     /locus_tag="VGR29_05690"
     CDS             532..963
                     /locus_tag="VGR29_05690"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEV2073118.1"
                     /translation="MTTAGLDVFDSTVQQTNEWLNEIAEELHTDNRREAYLALRGTLH
                     ALRDNLVVEEATDLAAQLPMLVRGIYYEGWNPSKTPVRDRDRDAFLERVGAALDRAQP
                     QGIYPEPAARAVFKVLSNHVSGGEIDQIRNVLHKDIRELLS"
     gene            1075..>2124
                     /gene="trpB"
                     /locus_tag="VGR29_05695"
     CDS             1075..>2124
                     /gene="trpB"
                     /locus_tag="VGR29_05695"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV2073119.1"
                     /translation="MSHEHRVYQATAQLPNANGYFGRFGGQFAPETLMPAISELIEAY
                     QEARNDPAFQAEIDRLATYYVGRPTPLYEATRFAGEAGGGNGPRIFLKREDLAHTGAH
                     KINNALGQALLAKRMGKNRIIAETGAGQHGVATATACALLGLKCIVYMGEVDIHRQSL
                     NVFRMRLLGAEVIPVTSGSKTLKDAINEAIRDWVTNVDDTFYIFGTVAGMHPYPMIVR
                     DFQSVIGREAKAQFAEQVGGLPDMIVACVGGGSNAMGLFYDFIPEKDVEIVGVEAGGH
                     GLEGTKHAATLVKGEEGVLHGSWSYILQDDNGQIIETHSISAGLDYPGVGPELSWMKD
                     SGRATFAAITDEEALE"
BASE COUNT          442 a          671 c          607 g          404 t
ORIGIN      
        1 ggaagtgcac catcatcatt ccgccagggt atttgcgtgc cccgaagagg atgttctccc
       61 agaagtcgca catgatcggc atatgcgccg cgagatccat ctgcaccacg tcgatgaaga
      121 tcggcccgag gacgggatcg gtaaatgcgc gcgtatagaa cgtctctacc aatcgtgcga
      181 tgtcctcgcg ggtctctata tcgtaacgat cctcttctgt agccaactcc actgctccgt
      241 ctcgatcctt cgtcgtgccg tatatctata tctgaatatc gtcccatgta acgctcattc
      301 gcgcattcaa ttccgtcaca ggacgccgac ccggatatcg ctcattaacc cgttccaatg
      361 gcacacgtcc tgcagcagat catctgggtg tggccccaca gtccgcagcg agggccatct
      421 gccacgtcac atctgttcct ctagcgcagg tgaccgaggt tcgcaccgac cagtgtgatg
      481 caggcagtaa ggcaagaggt gacccaccaa cgaccagcaa ggaggtaaat catgaccaca
      541 gctggattgg acgttttcga tagcacggtg caacaaacca atgaatggct taacgaaatc
      601 gcggaggagc tccataccga caaccggcgt gaagcctatc tcgcgctgag gggtacgttg
      661 cacgcactgc gcgacaacct tgttgtagaa gaagccaccg acctcgccgc ccagctcccg
      721 atgctcgtgc gcgggatcta ttacgagggc tggaaccctt caaaaactcc agtgcgggat
      781 cgagaccggg atgcttttct cgagcgggta ggcgctgcgt tggaccgggc ccagcctcag
      841 ggcatctatc cggaaccggc cgcgagggca gtctttaagg tcctgagcaa tcacgtcagc
      901 ggaggagaga ttgatcagat tcgcaatgtc ctccacaagg acatcaggga actcctgagc
      961 tgatacccca caaggcattt cgcctggctc tcgacggatt cggccattgc aacgcacact
     1021 agtgacagca atgttgctag tgtgcgtttt ccatgtgcgc aggagcgtat tactttgtca
     1081 cacgaacatc gcgtttacca ggctacggca cagcttccaa acgccaatgg ctacttcggt
     1141 cgcttcggcg gccagttcgc ccccgagacc ttgatgcccg ccatctcgga gctcatcgag
     1201 gcatatcagg aagcaaggaa cgaccctgcc ttccaggccg agattgaccg tctcgccacc
     1261 tactacgttg gtcgcccgac cccgctttac gaggccacgc gcttcgccgg ggaggccggt
     1321 ggcggcaacg gcccgcggat tttcctcaag cgcgaggacc tcgcccacac cggcgcccac
     1381 aagatcaaca acgccctcgg tcaggccctg ctcgcgaagc gcatgggcaa gaaccgcatc
     1441 atcgccgaaa ccggtgccgg ccagcatggc gtcgcgaccg cgaccgcctg tgcgctcctc
     1501 gggctcaagt gcatcgtcta catgggtgag gtggatatcc accgtcagtc gctcaatgtc
     1561 ttccgcatgc gcctgctcgg cgccgaggtc atccccgtca cctctggctc caaaaccctg
     1621 aaggacgcta tcaacgaggc gatccgcgac tgggtaacca acgtcgacga taccttctac
     1681 atcttcggca cggtcgccgg catgcatccc tatcccatga tcgtccggga tttccagagc
     1741 gtcatcggac gggaggcgaa ggcgcagttc gcggagcagg ttggcggtct gccggacatg
     1801 atcgtcgcct gcgtcggcgg cggctcgaat gcgatgggcc tcttctacga ctttatcccc
     1861 gagaaggacg tcgagatcgt cggtgtcgag gccggcggtc atggtctgga gggaaccaag
     1921 catgcggcca ccctcgtcaa gggcgaggaa ggcgtgctgc acggctcctg gagctacatc
     1981 cttcaggacg acaatggcca gatcatcgag acgcactcga tctcagccgg cctcgactac
     2041 cccggcgtgg gcccagagct gagctggatg aaggactccg ggcgcgccac cttcgcggcg
     2101 attaccgacg aggaggcact ggag
//
LOCUS       DASYWM010000140         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_624860, whole genome shotgun sequence.
ACCESSION   DASYWM010000140 DASYWM010000000
VERSION     DASYWM010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624860"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VGR29_05700"
     CDS             complement(<1..780)
                     /locus_tag="VGR29_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV2073120.1"
                     /translation="MSDLLFGALPLLQLDPVMPEDGGENMAVSLALAALAFVITIIFG
                     RPFITGLRQKKIGKKIRLELELHLGKSGTPTMGGLMVVITVVVLTLVFNMIGRESMLL
                     PIAVLIATSAVGAIDDMMTIVGVTKPGKEEGMAGRVKMGWLFLIGTTAAFVMHLPDPW
                     GLGLTNMYIPLVGQFDIGWLYVPIAAFAVVGMANAVNLTDGLDTLAAGTSAIAFVAYG
                     IIAYLQGQTGVVTFCFIMAGALMGFLWFNAYPAQVIMGDSGS"
     gene            complement(777..2210)
                     /gene="murF"
                     /locus_tag="VGR29_05705"
     CDS             complement(777..2210)
                     /gene="murF"
                     /locus_tag="VGR29_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV2073121.1"
                     /translation="MRRYELRDILAGTGGEYSTALPAEPVFLSLERDSRQVKRGDVFI
                     AVKGETHDGHIFVEKAAQNGASASIVSREWAQQHSDIGIPLILVDEPVAALQRWAAWR
                     RSKLNVRVVGITGSVGKTSAKESTAAVLGQRFRVHKSPGNFNNEIGLPLSLLDCPDDV
                     EVIVLEMGGAYAFGELTLLASIAKPDVGVVTNVYPVHLERMGTIEAIAQTKQELVEAI
                     PEDGLVVLNGDDPRVRSMIAAAKSRVITYGLQDPADVRAYDVKSEGLKGTTFWVEING
                     ERTFLKVPFVGGPGVQIALVALAVGHGFGMHISEILVGLQDPAIQVRLLFVPGPNGSQ
                     LIDDTYNASTPSMLAALSLLEQIPAKRKIAVLGDMRELGHVSEEEHRVVGGRAGDVAD
                     VLVTFGDLARVALEEVQGIERPEGRPLEVHAFGLDQREELIEFLRHELGEGDIALLKG
                     ARGLQMDAIVGEIRADAADSAKGDSRA"
     gene            complement(2207..2461)
                     /gene="mraW"
                     /locus_tag="VGR29_05710"
     CDS             complement(2207..2461)
                     /gene="mraW"
                     /locus_tag="VGR29_05710"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEV2073122.1"
                     /translation="MIAFHSLEDRLVKQFITRESATCICPPEQPVCTCDHTPRLRRIG
                     KPVKPDPSEISTNPRSRSAIMRIAERLPVPETTTMMRSTT"
BASE COUNT          523 a          795 c          679 g          469 t
ORIGIN      
        1 cgatccggaa tctcccatga tgacctgcgc cgggtaggcg ttgaaccaca ggaaacccat
       61 cagagcgcca gccattatga aacagaacgt caccacgccc gtctggcctt gtagataggc
      121 aatgatcccg taggcgacga aagcgattgc ggaagtcccg gccgcaaggg tgtccaggcc
      181 atccgtcagg ttgacggcgt tagccatgcc aacgactgcg aacgcggcaa tcggaacata
      241 gagccagcca atatcgaact gacctacaag cgggatatac atattggtca ggccgagccc
      301 ccagggatcg ggtaaatgca tgacgaacgc tgcggtcgtc ccaatcagga agagccatcc
      361 catcttgacg cgcccggcca tgccctcttc cttacccggt ttcgtgacac cgacgatcgt
      421 catcatgtcg tcgatggcac caactgccga ggttgcgatg agcaccgcaa tcggcaggag
      481 catcgattcc cgcccaatca tgttgaacac aagcgtcagc accaccaccg tgatgacaac
      541 catcaggccg cccatagttg gcgtacccga tttgccgaga tgcagctcga gctccaaccg
      601 gatcttcttg ccaatcttct tctgccggag acccgtgata aagggccggc cgaagatgat
      661 ggtgatcacg aaggcgagcg cggccagcgc cagcgagacg gccatgttct cgccaccatc
      721 ctcgggcata accggatcga gttgcagcaa tggcagcgcg ccaaacagga gatcgctcat
      781 gcccgcgaat cgcctttggc gctgtctgcc gcatcagcgc ggatctcccc gacaatcgca
      841 tccatctgca gcccgcgggc acccttgagc agcgcgatgt caccctcgcc gagttcatgg
      901 cgaaggaact caatgagttc ttctcgctga tcgaggccga acgcgtgaac ctccagcggc
      961 cgtccttccg ggcgctcgat accctgtact tcctccagcg ccacgcgagc gagatcgcca
     1021 aaggtgacaa gcacatcggc aacatcacct gctcggcccc caaccactcg gtgttcctcc
     1081 tccgagacat gtccgagttc gcgcatatct ccaaggaccg cgatcttgcg tttggcagga
     1141 atctgttcca gcaggcttag cgcggccaac atggagggcg tgctggcgtt ataggtgtcg
     1201 tcgatcaact gggatccgtt cggaccgggc acgaagagca ggcgaacctg gatagcgggg
     1261 tcctgcaatc caaccaggat ttccgagata tgcataccga agccatggcc aacggcaagc
     1321 gccaccaggg cgatctggac acccggacct cccacgaacg gcaccttcag gaatgtcctc
     1381 tcgccattga tctcgaccca gaatgtcgtg cccttgagtc cctcagactt cacatcatac
     1441 gcccgcacat cggcggggtc ctggagccca tatgtaatca cccgactctt tgcagcggcg
     1501 atcatgctgc gcactcgggg atcatccccg tttaacacaa ccaggccgtc ttccgggatg
     1561 gcctcgacca attcctgctt ggtctgcgca atcgcctcta tcgtccccat ccgctcaagg
     1621 tgcaccggat acacattcgt cacgacgccg acatcgggct tggcgatgct ggcaagcagg
     1681 gtaagctcgc caaaggcgta ggctcctccc atctccagca cgatcacctc gacgtcgtcg
     1741 gggcaatcga gcagggagag cggcaaaccg atctcgttat tgaagttccc cgggcttttg
     1801 tggacgcgaa agcgctggcc gaggaccgcc gcggtggact ccttggcgct ggtctttccg
     1861 acgctacccg tgatacccac cacccggaca ttcagcttgc tgcggcgcca tgccgcccac
     1921 ctttgcagtg cagccactgg ttcatcaaca agaatcagcg ggatgccgat atcagaatgc
     1981 tgctgtgccc actctcgtga cactatcgat gcagatgccc cgttctgggc agccttctcc
     2041 acaaagatgt gcccgtcgtg ggtctcaccc ttcacggcga taaagacatc ccccctcttc
     2101 acctgcctcg aatcccgctc aagcgagaga aagaccggct ctgcgggtaa cgccgtggaa
     2161 tactcgccac cagttcccgc caggatgtcg cgtaactcgt atcgtctcat gtggtggatc
     2221 tcatcattgt cgttgtctca gggacaggaa gccgctcggc aattcgcatg atcgcggacc
     2281 ggctccgggg attcgtactg atctcggatg gatcaggctt cactggcttg ccaatccgtc
     2341 gtaggcgagg agtatgatcg cacgtgcaga ccggctgctc cgggggacat atgcaggtgg
     2401 cagattcgcg cgtgatgaac tgcttgacga gccgatcttc aagcgagtgg aaggcgatca
     2461 cgacca
//
LOCUS       DASYWM010000141         9035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_99273, whole genome shotgun sequence.
ACCESSION   DASYWM010000141 DASYWM010000000
VERSION     DASYWM010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9035
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99273"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /locus_tag="VGR29_05715"
     CDS             <1..1693
                     /locus_tag="VGR29_05715"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2073123.1"
                     /translation="LGSGPRDLPARHRTMRDAIAWSYDLLDQNEQALFRQLSIFAGGF
                     SLDAADAVAGAGGEMAPVPSTFDVVASLVTQNLVIQDSVAGQARFRMLETIREFGLER
                     LEVAGEMGLVRHRATTWCLMLAEEAEQELIRGEQAKWLDRLEAEQDNMREALEWSLTC
                     GEAETGLRLAAALWRYWSARGDLREGYSWLERALARGSEASATQRGKALHHLGNLAID
                     LGDYAAARKHYEQSLIIRRNNGGPSEIAEPLNGLGLLTFYRGDYASAQRFHEESLSLR
                     RASDDAHGLGNSLVNMGDVAAAQGDYKRAETLYEEALAIRRQMGNANGIAYCLFNLGD
                     AAWGQGKYTVARRRLEESLALFREVGDRLGMGYALHSLGRVAHAEGNERQAAVFFGEA
                     LPLRQELGDRRGVIECVEGLASVASTHDPALAVSLYAAANALRTALNTPLRPTDNVVQ
                     EQELAAIRANLDTEAFDEAWAIGLPLSLEDAVAEASGIAARIGADRESLPAGLSNRES
                     EVLRLVATGLTNAQIAEALYLSPHTINAHLNRIYHKLGVTSRSGATRFALDHGIA"
     gene            2028..2564
                     /locus_tag="VGR29_05720"
     CDS             2028..2564
                     /locus_tag="VGR29_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073124.1"
                     /translation="MVGFALGLALAFGSAEHTGAQRDYGSCYSYATQESAQAALDTTT
                     DPIVVDILDHNDDGIACDNFDYAAGDYIDDGDAVMVVVCDESNGILVEVEEWIADGSL
                     HFPSHRATDAEIAAGECAVTAPVKTPPAEVPASGTTTAVSLPNTGTGASDSAPSWPAA
                     LLLALCTATAAGSTRRRR"
     gene            complement(2803..3786)
                     /locus_tag="VGR29_05725"
     CDS             complement(2803..3786)
                     /locus_tag="VGR29_05725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV2073125.1"
                     /translation="MSPLEQLNPMQRYLVHEFVEDYEDGLLSRRDMISRVLHITGSIA
                     AAATVLTSLGVQAAGAQGATPPPPNGPQSPLSVPEDDPRVLAANVTFAGADEAPVQAY
                     QAVPVEQLGEKLPLVLVCHENRGLTEHIRDVARRLAVEGYLACAVDLLSRQGGTEAVE
                     DPSEIPAVLTEGDMSRHVADFQAAIDHYEVSGEADMSRIGMTGFCFGGGITWRAATQI
                     EELDAAVAWYGPPPPLEEVPNIQAAVLGIYSDDPDDFANEGRDELEAALEEAGVTYEI
                     NVYPDTQHAFHNDTGQRYNEEQAIAAWQDMLAWFDQYLVGDMATPGATPEG"
     gene            complement(4044..4349)
                     /locus_tag="VGR29_05730"
     CDS             complement(4044..4349)
                     /locus_tag="VGR29_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073126.1"
                     /translation="MSGLEHRVPATVPADMAGRSSAEKYERVAFTFCKMGTIGLIAWV
                     LSPPLFVLIVALAAIGFYGRAISLGLTRSRCFLRKPTLIIGFWAFVALIDAAWLLNW"
     gene            complement(4346..4585)
                     /locus_tag="VGR29_05735"
     CDS             complement(4346..4585)
                     /locus_tag="VGR29_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073127.1"
                     /translation="MAKRLKRLTVKSFMDAWTLNVERLQQCCVHVGSTDGEADFVRVP
                     FCARQLFGDLRRKTSEGHVPAGQLIQLNGRKGVRR"
     gene            complement(4570..5898)
                     /locus_tag="VGR29_05740"
     CDS             complement(4570..5898)
                     /locus_tag="VGR29_05740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV2073128.1"
                     /translation="MLALRPYTPIRMVLTVCGRCFEEDPDRRIDYEMDILQGNLIMMD
                     GRVYLRRHCRRGHGEVTSLYEDSFDLWNDLQQWRVPTREVVPDTVANDAPIPMGYLNG
                     LGNLQTQHSCILLLDITENCNLNCPTCFAESGPGRGRFARLDHLMRSLDTAIEREGGR
                     IDVLMLSGGEPTVHPDIVEILEQAIARNVTRVILNTNGLRIVRDDRFMAALAKLRGRV
                     EVYLQFDGFALETHLYHRGQDLRDTKAEAVRRLKSERVFTTLAMAVAAGVNDREVGDV
                     ARFAFETDYIAGVAFQPVFGSGRANPIDPMNRATTTGVMQRLGEQTSGLVSPEDFIAL
                     PCSHPDCCAISYFVRGDEGQLNSIPRLLGRDVLKANLGLVSNRIAADSEMEAALLGMM
                     SETTTITRPELVDYVPISARAATSASANSSRHSVAASSGASGPASLWRSA"
     gene            complement(5916..6410)
                     /locus_tag="VGR29_05745"
     CDS             complement(5916..6410)
                     /locus_tag="VGR29_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073129.1"
                     /translation="MDSEITRYIRENRDRYTREAIEARLIRAGYSPTEIDALWPPDDE
                     QRPPVPQGRAFWRPFILSVIGMYGITFLVYAITALVWTDESIWPIILALLFLFLLSAG
                     SISVDIVRRKRSAWASGTASSSKAGVASGLLTALVIPFISLVIVAGLCSALTGLPFTG
                     SRFP"
     gene            complement(6510..7340)
                     /locus_tag="VGR29_05750"
     CDS             complement(6510..7340)
                     /locus_tag="VGR29_05750"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEV2073130.1"
                     /translation="MYPVLYRDGAMVLYTHDVFTALGLLVGLLLYYGELRWRGMLEFR
                     IFVISVMAIFGGGIGARIITAWEHLPYYSNQAGAPLTYLISHSGKSIIGGIVGGYIAI
                     VLAKRSLGYTHSTGDCYAAAIPLALVIGRVGCLLSELPLGTPTSLPWGVSVPESATRQ
                     FADCPGCQGTMHPSMVYEIIFHLSAFLLILRYRHLIPVRGDTLKLYLLIAAVFRFLVE
                     FVRANPDQVSGLSGPQIVLIPLTGLLVYHFVRQWQRGAYRMPPVPAPSRVTLREGEVA
                     "
     gene            complement(7473..8351)
                     /locus_tag="VGR29_05755"
     CDS             complement(7473..8351)
                     /locus_tag="VGR29_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV2073131.1"
                     /translation="MSSSSGAPRLFGIHTIQTWTWPELRDRWQWFESLGFDSLWLPDH
                     FFPTAGRDKPMFEAWTLLSGLALVTQRARIGILVSSNTFRHPAVLAKQAVTVDHLSEG
                     RLELGIGAGWFVEEHDVFGLDFPETKELVDRYAEAVQLIDRYLTHDQSSFKGEHYRLD
                     GAYNRPAPVQQPRPPLVMGAHGPRMLKLVARYADTWNSFAPVDELRRRNDQLTVYCEE
                     IGRNPASIKRSMFYGVNQSPEDDPWASSDAFEEFIGRYAEAGMEEFVLQPPKSERFDM
                     VERIASDVLPRLRKPE"
     gene            8758..>9035
                     /locus_tag="VGR29_05760"
     CDS             8758..>9035
                     /locus_tag="VGR29_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073132.1"
                     /translation="MMLTLYTFGDSILDCRWYNDAGLDPGQLLVRNDDRLFAEFRGQD
                     LSSRGPVQVEHRAVDGATVAGLPAQSRNLRPSGESVAILTVGGNDLLQG"
BASE COUNT         1791 a         2755 c         2781 g         1708 t
ORIGIN      
        1 gttgggcagc gggccccgcg atctgccggc ccgccatcgg acgatgcgcg atgccattgc
       61 ctggagctac gacctccttg accagaatga gcaggctctg ttccggcagc tgtcgatttt
      121 cgccggtggg ttttcccttg acgcggctga tgccgttgca ggtgccggtg gggagatggc
      181 accagttccc tccactttcg atgtcgttgc ttcactggtg acccagaacc tggtgattca
      241 ggattccgtt gcgggccagg cccgtttccg gatgctcgaa acaatccggg aatttggtct
      301 cgaacgcctt gaggtagcag gggaaatggg cctggtccgc catcgagcca caacgtggtg
      361 ccttatgctc gctgaagaag ccgagcagga gttgatccgg ggagaacaag cgaagtggtt
      421 ggaccgcctt gaggccgagc aggacaacat gcgcgaggcc ctcgaatggt cgctcacttg
      481 cggagaggcg gagacgggcc ttcgcctggc cgctgcactc tggcgctact ggtcggcacg
      541 cggcgatctg cgggaagggt acagctggct tgaacgcgca ctggctaggg gaagcgaagc
      601 atcagccact caacggggga aagcccttca tcaccttggc aacctggcga tcgaccttgg
      661 agactatgcg gccgcccgta agcattacga gcaaagcctc atcattcggc ggaacaacgg
      721 agggccatca gagattgccg aaccgctcaa tggtctcggc ctgctcacgt tctaccgggg
      781 tgactatgcc tctgcccaac gattccatga ggagagcttg agtctccgcc gtgcttccga
      841 tgatgctcat ggactcggga actctctggt taacatgggc gatgttgcgg ccgctcaggg
      901 tgactataag cgggccgaaa ccctgtatga ggaggctctg gcgattcgac gacagatggg
      961 caacgccaat ggcattgcgt actgtctctt caatctcgga gatgccgcct gggggcaggg
     1021 gaaatacacg gtggcgcggc gcaggcttga agagagcctc gcactcttcc gtgaggtggg
     1081 tgaccggctg ggaatgggtt acgcgctaca cagtctcggt cgcgtagccc atgccgaagg
     1141 aaatgagcgg caggctgccg ttttctttgg cgaggcgctt ccactccggc aggaactggg
     1201 cgacagacgg ggcgtcatcg agtgtgtgga agggctggca tcggtagcaa gtacgcacga
     1261 cccagcattg gcagtgtccc tctatgctgc agcaaatgcc ctgcgtacgg cactcaacac
     1321 ccctcttcgt cccaccgata acgtcgttca agagcaggag ttggccgcca tccgcgccaa
     1381 cctggacacg gaagccttcg atgaggcgtg ggcaattggc ttgcctctct cactggagga
     1441 cgcagttgcc gaagcgtcag ggattgccgc gaggatcgga gcggatcgag agtcgcttcc
     1501 tgccgggctt agcaaccgtg agagcgaggt cctgaggctg gtggcaacag gtctcacgaa
     1561 cgcgcagatc gccgaggcgc tctacctcag tccgcacacg atcaacgccc acctcaaccg
     1621 aatctatcac aagctgggtg tcacctcgcg cagtggcgcc acccggttcg ccctcgatca
     1681 cggcatcgcc tgacatcgtt tcatggcagc tgttaagatg ggctacagcc gacaaacacg
     1741 gatattgcct ctctccctat ccaaggcgcg tgctcttcct gtgcagcctt catcacaagc
     1801 cacgtgcaat taataaggac gattaatcaa ttctcgcgat gaagaggaac gccaacctcc
     1861 gtagagtgaa ggaacgggag atgtggtggg gccacgctcg gttccagatt ccgacagtga
     1921 tagcacggga ggttgtcgtg aagaagtcgt ttctacggcg tggtcgtggt gcatcgatgc
     1981 tcgccccaaa acggatactg gatgtgatct tgtggcgatg agtcattgtg gtgggcttcg
     2041 cgcttggtct tgcattggcg tttgggtctg ccgagcacac tggggcacaa agggattatg
     2101 gcagctgcta ttcgtatgcc acgcaggaga gcgctcaggc ggcactcgac accaccactg
     2161 atcccatcgt ggtggacatc ctggatcaca acgacgacgg tattgcctgc gacaatttcg
     2221 actatgccgc tggtgactac atcgacgacg gcgatgcggt gatggttgtg gtgtgtgatg
     2281 agtcgaacgg gatcctggtc gaagtcgagg aatggattgc cgacggctcc ctgcatttcc
     2341 cgtcccaccg agcgacggac gccgagattg cagccggtga atgcgcggtg acggctccgg
     2401 tcaagacacc ccctgccgaa gtccccgcca gcggaaccac gaccgctgtg tcgctgccca
     2461 acaccggaac aggagcgagt gattccgcac catcatggcc agccgctctt cttcttgctc
     2521 tgtgtacagc cacggcagct gggtcaactc gtcgacgacg ataggcgaaa gcgggaaaca
     2581 cgaagaacgg gtacctgagg tgagtatctc aggtacccgt tctctccctg gctgtcgata
     2641 ccatcattat cgtcccgaag ggtgaagaga aagacaactg gcaagagttc ggagccaacc
     2701 agtccatttg ccaatcgggt cgtgtcaaca actggcgtgg gagcgggggc acgtgtgaag
     2761 cagacgcggc ccatagtggg ccgcgtctgc aggtggcgag gatcagccct cgggtgtggc
     2821 gcctggggtg gccatgtccc cgacaaggta ctgatcgaac caggcgagca tgtcctgcca
     2881 ggcagcaatg gcctgttcct cgttgtatcg ctggcccgta tcgttgtgaa aggcgtgctg
     2941 tgtatccgga tagacgttga tctcgtaggt gacacccgcc tcctccagcg ccgcctccag
     3001 ttcatcgcgt ccttcattgg cgaagtcgtc cgggtcgtca gagtagattc ccagcacggc
     3061 tgcctggatg ttcggcacct cttccagcgg aggtggcggt ccataccacg ccaccgcggc
     3121 gtccagttcc tcgatctgcg tcgcggcgcg ccaggtaata ccgccaccaa agcagaaacc
     3181 ggtcatgcca atgcgcgaca tgtcggcctc gccgctgacc tcatagtggt cgatcgccgc
     3241 ctggaagtcc gcgacatgcc ggctcatgtc accctcagta aggaccgctg gtatctccga
     3301 tggatcttcc accgcctcgg ttccaccctg cctgctgagg agatcaaccg cgcaggcaag
     3361 gtatccctcg acggcaagcc gccgcgccac gtcgcggata tgctccgtga gaccccgatt
     3421 ctcgtggcag accagtacca gtggcaactt ctcgcccagt tgctccacgg gaaccgcctg
     3481 atatgcctgg actggcgctt cgtccgcgcc ggcgaaggtg acgttggccg ccagaacacg
     3541 cggatcgtcc tcgggaaccg agagcggact ctgtggacca tttggaggag gcggtgtcgc
     3601 cccctgagcg ccagcggcct gcacacccaa tgaggtgagc acggttgccg cggcagcgat
     3661 gctgcccgtg atatgcagca cgcggctgat catgtcacgc ctggagagca gcccgtcctc
     3721 atagtcctcc acgaactcat gaaccaggta ccgctgcatg gggttgagtt gctcgagtgg
     3781 tgacatccgt ccctcctctt ttggtacatc actccatcag cccggttgct gatgacctct
     3841 cgacgccaga acgccatcgt tgtaatggga acgccaaccg aaccgcgaat ccaggcgatg
     3901 agccggtttc catggtgtgg gaacgcgatc cttcgtgccg gtgcagtgag agtgagttcc
     3961 acactgccag caagcgcgtc gtatgtcaag cggagatgga caggtgtcgc tttacccaca
     4021 gacgacttgg ggttgcctgt ctatcaccag ttgagcagcc aggcagcgtc aatcagggcc
     4081 acaaatgccc agaagccgat gatcagtgtg ggcttccgca gaaagcagcg gcttcgcgtc
     4141 aacccaaggc tgatggcgcg accgtagaac ccgatggcgg ccagcgccac gattagcaca
     4201 aacaggggtg ggctgagcac ccaggcgatg agaccgattg tgcccatctt gcagaacgtg
     4261 aacgccacgc gctcgtactt ctcggcggaa ctccgcccgg ccatgtccgc cggcacggtg
     4321 gcgggcaccc gatgttccaa tccgctcatc gacggactcc tttccgtcca ttgagctgga
     4381 taagctgccc cgccgggacg tgcccctcgg aggtcttgcg ccgcagatcg ccaaagagct
     4441 gacgggcgca gaatggcacc cgcacgaaat ccgcctcgcc gtccgttgag ccgacatgga
     4501 cgcagcactg ctgcaggcgc tccacattca gggtccatgc atccatgaac gacttgaccg
     4561 tcagccgttt caggcgcttc gccacaagct cgccgggccg ctcgcgcccg aggaggcggc
     4621 taccgaatgt cttgatgaat tcgccgaggc cgaggtcgca gctctcgcag agattggcac
     4681 gtagtcgaca agctcagggc gggtgatcgt cgtcgtctcg ctcatcatgc ctagcagcgc
     4741 cgcctccatc tcgctgtccg cggctatgcg gttcgagaca aggccgagat tggctttcaa
     4801 gacatcccgt ccaagcaggc gcgggatcga gttcaactgc ccctcgtcgc cacgcacgaa
     4861 ataggagatt gcgcagcaat cgggatggct gcacgggagc gcgatgaaat cctccggcga
     4921 gacgagaccg gacgtttgct ccccgagccg ctgcataacg ccggtggtgg tcgccctgtt
     4981 catgggatcg atggggttgg cgcgacctga gccgaacacc ggctggaacg cgacaccggc
     5041 gatgtaatcc gtctcaaacg caaaccgggc gacatccccc acctcgcggt cattcacccc
     5101 ggcagcaacg gccattgcca gcgtggtaaa gacgcgctcg ctcttaagcc gtcgcaccgc
     5161 ttctgccttg gtgtcgcgca gatcctggcc ccggtggtag aggtgagtct cgagcgcgaa
     5221 gccatcaaat tgcaggtaca cctccaccct gccgcgcagc ttcgcgagcg cagccatgaa
     5281 tcggtcatcc cggacaatcc ggagcccgtt tgtgttcagg atcacccggg tgacgttgcg
     5341 tgctatggcc tgctcaagga tttcgacgat gtcgggatga acggttggct cgccgccact
     5401 gagcatcagc acgtcgattc gcccgccctc gcgttcgatt gcggtatcga gcgatcgcat
     5461 caaatggtca aggcgggcga agcgacctct gccggggccg ctctccgcga aacacgtcgg
     5521 acaattgagg ttgcaattct ccgtgatatc gaggagcagg atgcagctat gctgcgtctg
     5581 gagattcccc agtccgttca ggtagcccat ggggatcggc gcgtcgtttg cgaccgtgtc
     5641 cggaacgacc tcccgtgtcg gcacgcgcca ttgctgcagg tcgttccaca ggtcgaagct
     5701 atcctcgtac aggctggtca cctcgccgtg cccgcggcgg caatggcgcc ggaggtagac
     5761 tcggccatcc atcatgatca ggttgccctg caggatgtcc atctcgtagt cgatcctgcg
     5821 atcgggatcc tcctcgaaac atctgccgca aacggtcagc accatccgga ttggggtata
     5881 cggtctcaac gcgagcatgc ccagcctcct ggcaactacg ggaagcgtga tccggtgaac
     5941 ggcaaaccgg tcagggcgga gcacaggccg gcaacgatga caagggagat gaacggtatc
     6001 acgagagcgg tcagcagccc gctagccacc cccgccttgc tcgaactagc tgttcccgat
     6061 gcccaggccg accgttttcg tctcacgatg tcaacagaga tcgacccggc ggagagcagg
     6121 aagaggaaca ggagggccaa gatgatgggc cagatcgatt cgtcggtcca aacaagggcg
     6181 gtgatggcat aaacgagaaa ggtgatcccg tacatcccga tgaccgagag gataaaaggt
     6241 cgccagaatg cccgaccctg gggaaccggc gggcgctgct catcatccgg gggccacaag
     6301 gcgtcgatct cggtagggct atagcctgcg cgaatcaggc gagcctctat tgcctcccgg
     6361 gtatatcgat cgcgattttc cctgatgtac cgggttatct cagaatccat gatgggactc
     6421 cctccggtgc ctgctccagg cacccggatc actgacgacg ctatgacggt tcagaaccag
     6481 gaggtaaccc gcgagcgtga tttcctcgct tatgcgacct ccccctcccg gagcgtcacc
     6541 cttgagggtg ccggcactgg aggcattcgg taggctcccc gctgccactg ccgcacgaag
     6601 tgatacacga ggagtcccgt caggggaatg agcacgatct gcggtccact gaggccgctc
     6661 acctgatccg ggttcgcccg cacaaactcc acgaggaagc gaaaaacggc cgcgatcaac
     6721 aggtagagct tgagcgtgtc tccccgtacc ggtatgaggt gcctgtagcg caggatcagc
     6781 aggaatgcgg agaggtggaa gatgatctcg tagaccatgc tcggatgcat cgttccctgg
     6841 cagccggggc agtcggcgaa ctgccgtgtg gcgctctccg ggacggacac accccatggc
     6901 aagctggtcg gagtccccaa gggcaactcg gagagcaggc agcccacgcg cccgataacc
     6961 agtgccagcg gtatcgcggc ggcatagcag tcgccggtgg aatgcgtata cccgagcgag
     7021 cgcttggcga gcacgatcgc gatgtagccc ccgacgatcc cgccgatgat gctcttgcca
     7081 ctatgcgaga tgagataggt cagtggagcg ccagcctggt tgctgtagta cggcaggtgc
     7141 tcccaggcgg tgatgatccg cgcaccgatg ccgccaccga agatggccat cacggagata
     7201 acgaaaatcc ggaattcgag catccctcgc cagcgcagct ccccatagta gagcagcagc
     7261 cccacgagca gccccagtgc ggtgaagacg tcgtgtgtat acaggaccat cgcgccgtca
     7321 cggtagagga ccgggtacat tggcctcacc tctcatttcg aagggatcac acaatcatca
     7381 tagcgaatca cggagagcgc tcaatcatcg aggcgatacg ttatgccacc gcaggagcgt
     7441 cgcgacgctt cctatgcagt cgggtcggtg tcttactccg gcttccgtaa cctcggcagc
     7501 acgtcagacg cgatgcgctc gaccatgtcg aaccgctcgg acttgggtgg ctggagcacg
     7561 aactcctcca tcccggcctc ggcgtaccgg ccgatgaact cctcaaacgc atcggacgat
     7621 gcccacgggt cgtcctcggg cgactggttg actccgtaga acatggaccg tttgatgctg
     7681 gccgggtttc gccctatctc ctcgcagtac acggtgagct ggtcgttacg gcgacgtagc
     7741 tcatcgaccg gagcaaacga gttccatgtg tcggcatatc gtgctaccaa cttcagcatg
     7801 cgtggaccgt gcgcacccat caccagtggc gggcggggtt gctgcactgg ggccggacga
     7861 ttgtacgcgc cgtcaagccg gtagtgctca cctttgaacg aggattgatc gtgggtgagg
     7921 tagcgatcga tcagctggac cgcttcggcg taccggtcga cgagctcctt cgtctcgggg
     7981 aagtccaggc cgaagacatc gtgctcctcg acgaaccagc ccgcgccaat gccaagttcc
     8041 agccgacctt cggacaagtg gtcgacggtg accgcctgct tggcaagcac ggcagggtgg
     8101 cggaaggtat tgctgctgac gaggatgcca atccttgccc gttgggtgac gagggcgagg
     8161 ccagagagca aggtccacgc ttcgaacatg ggtttgtcgc gtcccgctgt tgggaagaag
     8221 tggtctggca gccagagact gtcgaacccc aggctttcaa accactgcca gcgatcacgg
     8281 agttcgggcc atgtccaggt ctgaatcgtg tgaattccga acagtcgggg tgctccggac
     8341 gaactcgaca tgattgctcc tcaccgtgcc cggttcagac tccaatgttg gcagtccagt
     8401 ggtgtctcgg gtagagacag agggcactgc taacgccgtt cagtcgctcc ctgtctcagg
     8461 cctggcaaag cccctacaga ggagcaaagg ctcgatcact cgtttttccc agtaagcgtg
     8521 attgtgacat ggacaccaag accccacaag ggcaccagca tggtttttcg tgcaacggtc
     8581 cttccagagg ggccgtgtca gatattgaag gacggccctg cgcatactcg attcgacgag
     8641 tgcgataagt cctgtttacc atgccattgc gtgccttgcg acctcgcccg cagtcagtca
     8701 gactaatttg aagacgcgtc ccgccaccct atagaacgaa acaccagcgc gaggcacatg
     8761 atgctcaccc tctacacgtt cggcgactcc attctcgact gccgctggta caacgacgcc
     8821 gggctcgatc ccgggcagct tctggttcgc aatgatgacc gcctcttcgc ggagttccga
     8881 gggcaggatc tctcctcgcg cggaccggtc caggttgagc atcgtgccgt tgatggggca
     8941 actgttgccg gtctgccagc acaaagccgg aacctgcgcc cttccggcga gtcggttgcg
     9001 atcctgaccg tcggcggcaa cgacctgctc caggg
//
LOCUS       DASYWM010000142        10652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_456174, whole genome shotgun sequence.
ACCESSION   DASYWM010000142 DASYWM010000000
VERSION     DASYWM010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456174"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1837)
                     /gene="mutS"
                     /locus_tag="VGR29_05765"
     CDS             complement(<1..1837)
                     /gene="mutS"
                     /locus_tag="VGR29_05765"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV2073133.1"
                     /translation="MTSVTTDDKQALSKEVVPMRRQYLQIKSRYPEHILFFRLGDFYE
                     TFDGDAEIVAGVLDIVLTGREMGKGQRVPLAGIPYHAADGHIARLISAGYKVAICEQI
                     GEPTKGRGLVERNVTRVITPGTIVDPAMLDARTNNYIAAAVIEGNRAGIAFADITTGE
                     FQTAEIREETAEETLLALGREILRLRTPEIVIPADMTDSMALPRYSWLPESARLSKTD
                     AWRWTEERAAHSLMRHFEVDSLDGFGATGKPFAIRAAGGLLQYLEDTQLSGLEQITAL
                     GTYSTDGFMTLDAQTRRNLELAESARGDRKLSLTAVLDHTRTPMGARLLRRWINQPLL
                     DVNALDERLDAVEWFTRQPVRRAQIRDLLGEVGDIERLANRITTGVAMPRDLGQLRGA
                     LVVLPDLSPLAHGLPGIPALPDVGAAAEILTGALVDEPPAVLGKGAVFRPGYASELDG
                     HRARAREAREWIANLERTERDRTGIKSLKVGYNKVFGYYLEITAAALANAEKERLTGG
                     SDAPALPLDYIPKQTLANATRYFTAQLKEYETLVLTAEDTLNDLEADAYRHLLKQLAS
                     SCDRMLQAAAIIAQIDAAANLAEVAVDRQYVRPVLSNDRSLEIVDG"
     gene            2033..2839
                     /locus_tag="VGR29_05770"
     CDS             2033..2839
                     /locus_tag="VGR29_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2073134.1"
                     /translation="MAEEHELKRLIEEAEASGGAVGVAIDSTSGWTFQHRGDRRFRAA
                     STVKIPIMIEIFRRVDQGEIALTDRYVVERHDHSVGSGVLHELHAGLAVTIQDLLYLM
                     MSISDNTATNILIDMAGMKNVNATMRELGMEQSTLGRKMQGKPAEGEQHENWATAAEY
                     ASLVGKLLGNQVASPDACAQMLAILEKQQNTRRIGRYMPQDGLRWGSKTGSILGVTND
                     VGFATSEAGMVIISVFCEDLPDMHVSEKLIGDVARQAVIGAGIATPLFTS"
     gene            2999..3277
                     /locus_tag="VGR29_05775"
     CDS             2999..3277
                     /locus_tag="VGR29_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV2073135.1"
                     /translation="MPLFAAMLQYGEDAERRQEVRPSHREYQRGLMEAGKLLHAGPYV
                     DDTGAIVIYDAADLSEVQQLLTNDPFAQNGIIEAATIKEWTIVISRDL"
     gene            3653..5254
                     /locus_tag="VGR29_05780"
     CDS             3653..5254
                     /locus_tag="VGR29_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2073136.1"
                     /translation="MTSRKLGEAIPMRSNRREFTLGMAGAAGALALGSQPTSAQDEVE
                     IPEGGTLQVAIIGEPPAVADAVFTTATVTNNVSQQIFETLFAFDSSFTPQPMLIEDYE
                     GSEDGLEFTFKLRTDVPFHNGDPMTAEDVVASLNRWGEINGRGKLIYGRMDSIEATDA
                     STVKMVFNQPSGVLPSFLARSEAMITPASVAEAAGTDQIPEDQLIGTGPFKFIEHQVD
                     RYLRLGRFDDYAARNDPPNGLAGKRIPYLDGIDFVPVPDESVRANGLITGEYHFSDVL
                     PSDLHEMLEMDPAVDPIIVQPYYWYSPHFNKAQGLFTNPALRQAVQLCFSESEAMLAG
                     FGQEEFVRFDPSVCGEETPWYSTAGADVYDRVDLEQARALLKEGGYNGETVRWLATRE
                     YPYNFLMADFTRQGMEAIGMKVELIVSDWATLVQNRAKPDAYEIFLTGHSQYSHPATQ
                     PFNDPAWPGFWESEAKDTLVNEMVAETDLEKLKSIIDEYTALIWDEMPFVKCGDNFVL
                     RGIRQEVVGYNNMPDWFFWNLGLNG"
     gene            5341..6291
                     /locus_tag="VGR29_05785"
     CDS             5341..6291
                     /locus_tag="VGR29_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073137.1"
                     /translation="MAGFLVRRFVAIIPVMLLVATAVFLLLKLTPGDPVGVILGPDAT
                     EERRQALRDDLGLNDPLPEQYLRWLGDAVRGDLGDSLFLNEPVLSALADRAEPTLLLT
                     LLSTLVALSIGLPAGIIAAQNRGKWLDMGTMAIAIVGISMPTFWLGLILILVFSVQLR
                     WLPVAGYAPLSDGLWESLRYLILPSVTLGVAQAALLARMTRSMMLEVLGAEYVLVARA
                     KGLAERRVVYLHALKNAIIPLINVAGLIFAALLGGAVVTEQIFNIPGIGRLLIQAVGR
                     RDVTLVQGSVLVIAAIYVLVNLLVDIISAGLDPRLRKTEG"
     gene            6295..7176
                     /locus_tag="VGR29_05790"
     CDS             6295..7176
                     /locus_tag="VGR29_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073138.1"
                     /translation="MATTVNDVRAATWTPSRFRRMGTVFGRHRSMVIGGVLLSLIVLM
                     ALLAPWLMTHDPTFQEPRERLIAPFGDYLLGTDAQGRDIYSRVVKGAQLSLLVGVCVS
                     IATSLVGGFIGLLTGFVPRIDAPIMRVMDGLMAFPGIVLAIGIMAVRGAAISNVILAL
                     TVVSTPRMARVVRSVVLSLARSQFVEAAESSGCSMWRVLRVHLLPNCMSPLIVQASFV
                     FAEAVLGEATLSFLGAGAPPSVPSWGGMLSESRLYLSQAAWTMIVPGAALTVTVFALN
                     LLGDGLRDMLDPKLRHR"
     gene            complement(7354..8352)
                     /locus_tag="VGR29_05795"
     CDS             complement(7354..8352)
                     /locus_tag="VGR29_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2073139.1"
                     /translation="MSTYDVAKIDQLARDSINGEERPDVPEEITTQMEPELEIEPDLE
                     RDLDVLQRLDPNFINDPVRLYLREIAETALLTHQQEIELAKRVEDNDLHAVQQFVRAN
                     LRLVVSIAKRYVNRGLTLLDLIQEGNIGLMRAVQKYDWRKGFRFSTYATWWIRQAITR
                     AIADQSRTIRLPVHMGDSISRYRKTLNQLAQQLGRQPTPEEVAEAMEVAPEKIHQIVQ
                     AAQRTISLETPIGNEDDTSLGDLIADEVSETPYEAASESMLKRDVSAALDTLSARERL
                     VLQLRFGLGQGHQHTLAEVGEQLQISRERVRQIENEALQKLRRMDGDRLFAYHQEL"
     gene            complement(8779..9147)
                     /gene="erpA"
                     /locus_tag="VGR29_05800"
     CDS             complement(8779..9147)
                     /gene="erpA"
                     /locus_tag="VGR29_05800"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEV2073140.1"
                     /translation="MITMTPEAITQLKDFLSDQGTPEHALRVFVAPGGCSGLQYGMTI
                     DEASDEGDEIIETNGVKVLVDNFSAMYLEGAEIDFVNSLMGGGFTVRNPNAVASCSCG
                     HSFDTGDDAAATAQGCGCGD"
     gene            9475..10422
                     /gene="rsgA"
                     /locus_tag="VGR29_05805"
     CDS             9475..10422
                     /gene="rsgA"
                     /locus_tag="VGR29_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV2073141.1"
                     /translation="MTEVPADAVPAIVVRAHGLWFEVKLLEGPRKHDVLIATLRGQLK
                     KHRRRTDIVAVGDRVYVVELPDNEAAIEAVGPRTRALVRTARNTRDTEQVILANPDQV
                     MFVFAIVDPEPHVRMLDRFIILAELQEIPVRIVVNKAELDEPQSERSIERIFGVYRDL
                     YPLHVVSAHTGEGIQELTQALQGKTTAVAGPSGVGKSSLLNAIDPGNTREIGDVSTAT
                     GKGRHTTIGSRLHQIGDDTFVADTPGMRSLAMHAIPAERLDTCFIEMRPFLGQCFYQD
                     CTHVHEPACAVRAAVDAGEISQQRYESYVALRTGDEVSS"
     gene            10449..>10652
                     /locus_tag="VGR29_05810"
     CDS             10449..>10652
                     /locus_tag="VGR29_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073142.1"
                     /translation="MGVQAHDTGPNGVTVDELIEKMLPFVEGLLRRIDGEEFTTNDFI
                     GLMLQVPETAAAYEAGMRAWGENR"
BASE COUNT         2151 a         2927 c         3287 g         2287 t
ORIGIN      
        1 gcccgtccac aatctccaga gaacgatcgt tactgaggac aggacgaaca tactgccgat
       61 cgactgcgac ctctgccagg ttggccgccg cgtcgatctg cgcgatgatt gccgctgcct
      121 gtagcatgcg gtcacaggaa ctggcgagtt gcttgaggag gtggcgatag gcgtccgctt
      181 cgagatcgtt gagtgtgtcc tcggcggtga ggacgagggt ctcgtattcc ttcaactgcg
      241 ctgtgaagta ccgagtggca ttcgccagtg tttgcttggg gatgtagtcg agtggcaggg
      301 cgggcgcatc cgagcctccc gtgagacgct ctttttccgc attggcaagg gccgccgccg
      361 tgatttccag gtagtagccg aagaccttgt tgtacccgac cttcagcgac ttaatgccgg
      421 tgcggtcgcg ctccgttcgc tcaagattcg ctatccactc gcgtgcttcg cgggcacgtg
      481 cccgatgacc atcaagctca ctcgcgtatc cgggccgaaa aaccgcccct ttgcccaaaa
      541 cagcgggggg ctcatcgacg agggcaccgg tcagtatctc ggcggcagcg cccacatccg
      601 ggagcgcggg tattcccggg agaccgtgtg caagtggcga gaggtccggt agtacgacca
      661 gcgcgccgcg caactgaccg agatccctgg gcattgcgac gcccgtggtg atcctgttcg
      721 cgaggcgctc gatatcgccg acttctccca ggaggtcgcg aatctgggcg cgtcgtaccg
      781 gttgtcgggt gaaccactct acagcatcca gccgctcgtc gagtgcattc acatcgagca
      841 gtggttggtt gatccacctg cggagcaagc gcgcgcccat tggcgtgcgg gtgtggtcga
      901 ggacagccgt aaggctcagc ttccggtcac cgcgagcgct ctctgcaagc tcaaggttgc
      961 gacgagtctg ggcgtccagc gtcatgaaac catccgtaga ataggttcca agagccgtaa
     1021 tctgctccag tccggagagc tgggtgtcct ccaggtattg caagagaccg ccagcggcac
     1081 gaatggcaaa cggtttgcca gtagccccga aaccatcgag cgagtcgact tcgaaatggc
     1141 gcatgagcga gtgggccgct cgttcttctg tccacctcca tgcatctgtc ttactcagcc
     1201 tggcggactc cggcaaccag gaatatcgtg gtagtgccat ggaatcggtc atatcggccg
     1261 ggatcacaat ttccggtgtc cggagacgca agatctcccg accaagcgca agcagcgttt
     1321 cttccgccgt ctcttcccga atctcggcgg tctggaattc accggtcgtg atatcggcaa
     1381 aggcaatacc tgcccggtta ccctcgatga cggctgcggc aatgtagttg ttggttcggg
     1441 cgtcgagcat tgccgggtcg acaatcgtcc ccggagtaat gacacgggtg acgttccgct
     1501 caacgagtcc acggcccttt gtaggctcac cgatctgctc gcagatggcg accttatagc
     1561 cggcagagat gaggcgggca atatggccat ccgccgcatg gtaggggatc ccagccagtg
     1621 gtacccgctg gcctttgccc atctcgcggc ctgtcaagac gatgtcgagc acgcccgcca
     1681 cgatctcggc atcgccgtcg aacgtttcgt agaaatctcc caaccggaag aagaggatat
     1741 gctccggata gcgtgacttg atctggagat attgacggcg catcggcaca acctctttcg
     1801 agagtgcctg tttgtcgtct gtcgtaaccg aggtcatatc gtcgaattat ctccacgctc
     1861 ggatcgtcgc tatcggtgct ccccaagtca aggatagcgt gttgcaggtg tacggtcacg
     1921 tcgaatcagg agtgctctgc gagcgagatc cagattcaga atcgttctgt taccctgctc
     1981 cgatcgacat gctgctgcat ggatcacacg aatcacaagg gaggaggctg ttgtggctga
     2041 agagcacgag ctcaaacggc tcattgagga ggcggaggcg tccggcggtg cggtcggggt
     2101 tgcaattgat tcaactagtg ggtggacgtt ccaacaccgg ggagacagac gcttccgcgc
     2161 cgccagcact gttaagattc cgatcatgat cgagatcttc cgacgagtcg atcaaggcga
     2221 gatagcgctg actgatcgct atgtggtcga gagacacgat cacagtgtgg gaagcggtgt
     2281 gttgcatgag ttgcacgcgg gtttggctgt cactatccag gatcttctct acctcatgat
     2341 gagcatcagc gacaatacgg cgacaaacat cctcatcgac atggcgggga tgaaaaacgt
     2401 caatgccact atgcgagaac tcggcatgga gcagtcaaca ttgggccgaa agatgcaggg
     2461 gaagcctgcg gagggcgaac aacacgagaa ctgggcgacc gcggcggagt acgcctcgct
     2521 tgtcgggaag cttctcggca atcaggtcgc ctcgcctgat gcctgtgccc agatgcttgc
     2581 gatcctcgag aagcagcaga acacgcggcg gattggccgg tacatgcctc aggacgggct
     2641 tcgctgggga tcgaagacgg gttccatcct gggtgtcact aatgatgtgg gatttgccac
     2701 ttctgaggca ggcatggtca tcatcagtgt cttctgtgag gaccttcccg atatgcacgt
     2761 gagcgagaag ctcatcggag acgttgcacg gcaggccgtc ataggcgcag gaatagcaac
     2821 gcccctcttc accagctagc gattgtgatg ccagccctgt cctgaagcgt gtcgttgggt
     2881 tcgatgtgtg ccgatcgcaa agtgagcatc cgattgcgac gggatgcgct atcgggtacc
     2941 gtgtcgttga cgaacaggtt ctccatccgg agagatgaga gggaagggga ggcgcaccat
     3001 gccattgttt gccgcgatgt tgcagtacgg ggaggacgcg gagcgccggc aggaagtgcg
     3061 gccatcgcat cgcgagtatc aacgagggct catggaagcc ggcaagcttc tccatgcggg
     3121 tccatatgtg gacgacactg gagcgatcgt catctacgat gcggctgacc tctcggaggt
     3181 gcagcaattg ctgacgaatg acccattcgc ccagaacggg atcatcgaag cagcaacgat
     3241 caaggaatgg acgatcgtga tatcgcggga tctgtgaacc cgcgtgttgg gcggcgacgg
     3301 gcatgtactc gaaaaaccct gcgggcgcca tctgatgtgc aagagaggcc ggttcccatc
     3361 gaaccggcct ctcttgccgt gaatggattg atgtacaggg gcccagggtt cccagtcata
     3421 gggaaagagg cgaccgccct ccatgtaacg aagcttctct gtttgcctgg ctcgttctcg
     3481 atctacggcg gctctcgaag gtgggcggga gccaggaagt gtccacgaga ttctatgagc
     3541 gggctttaca tctcatacat ccttgggtaa ggtgagcgga ctcgacaacg tcgtcaacgg
     3601 ttatcgtctc gataggagcg tcggaacgct ctaccaggta aggagaattt gtatgacctc
     3661 gcgcaaatta ggtgaagcga ttccgatgcg aagtaaccgt cgagaattca cgctcggcat
     3721 ggcaggagcg gccggcgcac tcgccctagg ctcccaaccg acatctgcac aggacgaagt
     3781 cgaaattccg gaggggggaa cgctacaggt tgcgatcatt ggggaaccgc cagctgtagc
     3841 agacgcggtg ttcaccaccg ccacggtgac aaacaacgtc tcgcaacaga tatttgagac
     3901 cctctttgcg ttcgattcca gctttacgcc ccagccgatg ctcatcgaag actatgaggg
     3961 aagcgaagac gggctggagt tcacctttaa gctgcggact gatgtcccct tccacaacgg
     4021 tgatccgatg acggcggagg acgttgttgc ctccctcaac cggtgggggg agatcaatgg
     4081 tcgagggaag ctgatctacg gtcgtatgga tagcatcgag gcgacagacg catcgactgt
     4141 gaagatggtg ttcaaccagc cttcgggtgt gcttcccagc ttccttgccc gttcggaagc
     4201 catgatcacc ccggcatcgg ttgctgaagc cgccgggact gatcaaatcc cggaagacca
     4261 gttgatcggt acagggccat tcaagtttat tgagcaccag gtcgatcgct atctccggct
     4321 tggtcgcttc gacgactatg cggcacgaaa tgatccgccg aacggattgg cgggaaagcg
     4381 cattccctat cttgacggta tcgattttgt gcctgtacct gatgaatcgg tgcgggcgaa
     4441 cggtctcatc acgggagagt atcacttctc ggatgtgctc ccgtcagacc tgcatgaaat
     4501 gctggagatg gacccagccg tcgatcctat tatcgtccag ccctattact ggtacagccc
     4561 gcatttcaac aaggctcagg ggctctttac gaacccggcg cttcgacagg cggttcagct
     4621 ctgcttcagc gagagtgagg ccatgcttgc cgggttcggg caagaagaat ttgttcggtt
     4681 cgatccgtca gtttgcggtg aggaaacacc gtggtacagc accgcaggcg ccgacgtgta
     4741 cgacagggtc gatcttgagc aggcgcgtgc tctcctcaag gaaggtgggt acaacggtga
     4801 aaccgtgcgc tggctggcaa ctcgggaata cccgtataac ttcctcatgg ccgatttcac
     4861 gcgtcaaggg atggaagcaa tcggcatgaa ggtcgagttg atcgtttcgg actgggcaac
     4921 cctcgtgcag aatcgcgcaa agccagacgc gtacgagatc ttcctgaccg gccacagcca
     4981 atacagtcac cccgcgacgc agccttttaa cgatcccgcc tggcccgggt tctgggaaag
     5041 cgaggcaaag gataccttgg tcaacgagat ggtcgcggaa acagatctcg aaaaactcaa
     5101 gtcgatcatc gacgagtaca ccgcgctcat ttgggacgag atgccgttcg tcaaatgcgg
     5161 tgacaacttt gtgctccggg gtatacgtca ggaggtcgta ggatacaaca acatgccgga
     5221 ctggttcttc tggaaccttg gtctgaacgg atagcgcacc ataacaccac acgaccgccc
     5281 tgtaacgtcg ttccggctgc agggcggtcg tgtgtttgga agcgggggga ggtacgctga
     5341 atggccggct ttctggttcg gaggttcgtc gcgatcattc cggtcatgct cctggtagcg
     5401 accgctgtgt ttctcctgct caaattaacg cctggagatc ctgtcggggt cattcttggg
     5461 ccggatgcaa cagaagagcg taggcaagcg ctgcgggacg atcttggcct caacgatccg
     5521 ctccccgagc agtacctgcg atggttgggc gatgcagtgc ggggcgatct tggagactcc
     5581 ctctttctga acgagccggt cctgagtgca cttgcggatc gagcggaacc caccttactc
     5641 ctgaccctgt tgtcaacgtt ggtggcactc tcgatcggac ttcctgccgg catcattgcg
     5701 gcacagaaca ggggcaagtg gctcgacatg gggacaatgg cgattgccat cgtaggcata
     5761 tcgatgccaa cgttttggct tggcctgatc ctgattctgg tgttctccgt gcagctcagg
     5821 tggctaccag tcgctggata tgcgcccctc tctgatgggt tgtgggagtc gctccgctac
     5881 ctgattctcc cgtcggtcac gcttggcgtt gcgcaggctg ccttgctggc ccgaatgacc
     5941 agatcaatga tgctcgaagt gctgggagcc gagtatgtgc tggtagcacg cgcgaagggc
     6001 ctggcggagc gtcgtgtcgt ttatcttcat gcgctcaaga acgccatcat tccgctcatc
     6061 aatgtggctg gactcatatt cgcggctttg ctcggtggcg cagtggtgac cgagcagatt
     6121 ttcaacattc cggggattgg ccggctgctc atccaggccg ttggtcggcg agatgtcaca
     6181 cttgtgcagg gaagcgtcct cgtgattgcc gcgatatatg tgctggtcaa cctgttagta
     6241 gacattatct ctgctggatt ggatccacgc ttacggaaaa cagaggggta ggacgtggca
     6301 acgacggtca acgacgtacg agcggctacc tggaccccca gtcggtttcg ccgaatgggc
     6361 acggtgtttg gcaggcaccg aagtatggtg atcggtgggg tacttctctc gcttatcgtg
     6421 ctcatggcgc tccttgcgcc atggcttatg acgcatgatc caacgtttca ggaaccgcgt
     6481 gagcgactca ttgctccttt tggagactac ctcctcggta cagacgccca gggaagggat
     6541 atctacagcc gggtggtcaa gggagcgcag ttatcattgc ttgtgggagt ttgcgtctcc
     6601 attgccacgt cactggtagg gggctttatt ggtctgttga cgggatttgt gcccaggatc
     6661 gacgcaccga tcatgcgcgt catggacggg ttaatggcct tcccgggaat cgttctcgca
     6721 atcgggatca tggcggtacg cggagcggcg atcagcaatg tcatcctcgc gctcacggtt
     6781 gtttctaccc cgcggatggc cagggttgtt cgttctgtcg tgctgagttt ggccaggtct
     6841 caatttgtag aggcagcgga aagcagcggt tgtagcatgt ggcgcgtgct tcgggtgcac
     6901 ctgttgccaa actgcatgtc cccactcatt gtccaggcaa gctttgtttt tgcggaagcg
     6961 gtgctagggg aggctaccct gtcgtttctt ggtgccggtg ctccgccatc ggtgccatcc
     7021 tggggaggga tgctgagcga atccagactc tatctaagcc aggcggcatg gaccatgatc
     7081 gtacccgggg ccgctttgac agttacagtg tttgctctca atctgctcgg cgatggattg
     7141 cgggatatgc tcgatcccaa gctccgtcat cgttaacgcg ctcacggccc cgttgctgtc
     7201 cgggcggcgc tgcgtaaatg acaagcgcct ggaggaacca gggcagtcgc gtagggaaat
     7261 ctcgctggtg ttcggtgaac ttctggagcg gaaagcaaac ggggaggcga tctaaccgcc
     7321 tccccgtttg acgctgatcg ttgaatgtag tccctagagt tcttggtgat acgcgaagag
     7381 acgatcgccg tccatgcgac gaagcttctg cagcgcctcg ttctcgatct ggcgaacgcg
     7441 ttcgcggctg atctggagtt gctctccgac ttccgccaac gtgtgctgat ggccctgtcc
     7501 caaaccgaag cggagctgga gcaccagtcg ctcacgggcc gagagagtat cgagcgcagc
     7561 ggagacatcc cgcttcagca tcgattcgct ggccgcctcg tagggagttt ccgatacttc
     7621 gtcggcgatc aggtcaccaa ggctggtatc gtcctcgttg ccgatcgggg tctcgagcga
     7681 aatggtccgc tgggcagcct ggacgatctg gtgaatcttc tccggcgcca cttccatggc
     7741 ctcagctact tcttccgggg tgggctgacg gccgagttgt tgagcaagct ggttcagggt
     7801 cttgcggtaa cgggaaatcg agtcccccat atgaacggga aggcgaatgg tgcggctctg
     7861 gtcggcgatg gcgcgggtga tggcctgacg aatccaccag gtggcatacg tgctgaaccg
     7921 gaagccctta cgccagtcgt acttctgaac cgcacgcatc agtccaatgt tgccctcctg
     7981 gatcagatcc agcagggtca aaccacgatt gacatagcgc ttggcgatgg agacgacgag
     8041 gcggaggttg gcacggacga actgctgcac agcgtgaagg tcgttatcct caacacgctt
     8101 ggccaactca atctcctgct gatgggtcag gagagcggtc tccgcgattt cgcgaagata
     8161 gagacgcacc gggtcattga taaagttggg atcaaggcgc tggagaacgt cgaggtctcg
     8221 ttcgagatcc ggctcgattt cgagctcggg ttccatctgc gtggtaatct cttcagggac
     8281 atctggtctc tcttcgccgt tgatgctgtc cctggcgagt tggtcgatct tggccacgtc
     8341 gtaggtcgac atctcaatta ctcttttctc ttcagtgcac gcggttaacg gcacgcaact
     8401 acgcacacgg atgatctatc agcgtgcgct acggttatcc cgtttatctc gcagggtatg
     8461 ttgcgccggg tggttgccgc caaatgtcct cgactgaacg atcaaggcat ctgctcctcg
     8521 agcatatgtg cgaaatggag cggcccttga tgtggtgctt cgatcgtgcg gatgatcgga
     8581 cgggctccac cgctggatgc tttggcgagc cacggcataa tcggaagaca tgctttcgca
     8641 tgccggacac catattataa cctactgtta agcgtttgtg aagtactgaa caatctggga
     8701 acgggaggcg aacctgcgcc gcccaaagca gatcttggtg acgcaagaga caggcccgac
     8761 gggatgatgt gcaacagatt agtcgccgca gccacatccc tgggcagtgg ctgccgcatc
     8821 atcgcctgta tcaaaggagt ggccgcagga gcaggaagca accgcgttgg gattgcggac
     8881 cgtgaagccg ccgcccatga gggaattcac gaagtcgatc tcggcgccct caaggtacat
     8941 agcgctgaag ttatcgacga gtaccttgac gccgttggtt tcgatgatct catcgccctc
     9001 gtcagaagct tcatcgatgg tcatgccgta ctggagaccg gagcagcctc ctggagcaac
     9061 aaacacgcgg agcgcgtgct ccggggtgcc ctggtctgaa agaaaatcct tgagctgcgt
     9121 gatggcttcc ggggtcattg taatcattgg atgtcttgcc tccggcaatc tataaacgca
     9181 tggcgttatc gggagcggac ttgcaccgct tccattacgt aagcatatcc ggaacgccac
     9241 gggtgtcaag catggctgga agttatatcc gatgccgatg cacggttccg tattgaccct
     9301 tgctctcata ataggttgca gagcgtcaga ggcttattga cgctctccgt gctcgtcgtt
     9361 cggtccggcg cttatccagg agattcgtgc ttgcccaaag cgcaaccccg tcgatcctcc
     9421 cctcgccaca gagccgattc tcgcggcgac cagacaggag cagccgcacc tccggtgacg
     9481 gaggtgccgg ccgatgccgt gccggcgatc gttgtccgtg cccatggtct ctggtttgag
     9541 gtcaaactgc tggaaggtcc gcgcaagcac gatgtgctca ttgccaccct gcggggacaa
     9601 ttgaagaagc atcggcgccg cacggacatt gtggcggtag gtgatcgcgt gtatgtagtg
     9661 gagctgccgg acaacgaggc tgcgatcgag gcagtggggc cgagaacccg agccctcgtg
     9721 cggacggcgc ggaacacgag ggatacggag caggtcatcc tcgccaaccc tgaccaagtg
     9781 atgttcgtct ttgcgattgt cgatccggag ccgcatgttc gcatgctcga ccgcttcatc
     9841 atccttgcag aactgcagga aattccggtg agaatcgttg tcaacaaggc cgagctcgat
     9901 gagccgcagt cagagaggag catcgagcgg atctttgggg tctatcgcga tctgtatccg
     9961 cttcatgttg tcagcgcgca taccggcgag ggaattcagg aactgacaca ggcgctccag
    10021 ggtaagacga cggcggtcgc cgggccgtca ggcgtcggca aatcgtcact gctgaatgca
    10081 atcgatcccg ggaatacccg ggagattggc gacgtctcca cggcgacggg caaagggcgg
    10141 cacaccacaa taggctcccg cttgcaccag attggcgacg atacctttgt cgccgacacg
    10201 cccggcatgc gttcactcgc tatgcatgcg atccctgcgg aacgcctcga tacctgcttc
    10261 attgaaatgc ggccctttct cgggcagtgt ttttatcagg actgtaccca tgtgcacgag
    10321 cctgcatgtg ctgtgagagc agcagtggat gctggcgaaa tcagccaaca gcgttacgag
    10381 agttatgttg ccctgcggac gggtgacgaa gtgtcttcct gagggatcaa ccagcaagga
    10441 gcaaccgagt gggagtacag gcgcacgaca cgggcccgaa cggtgtgaca gtcgatgaac
    10501 tgatcgaaaa gatgcttccc tttgtggaag gacttcttcg acggattgac ggcgaggagt
    10561 tcacgaccaa cgactttatc ggactcatgc tgcaagtacc ggaaaccgct gctgcgtatg
    10621 aggcgggaat gcgcgcgtgg ggcgagaacc gg
//
LOCUS       DASYWM010000143         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_61647, whole genome shotgun sequence.
ACCESSION   DASYWM010000143 DASYWM010000000
VERSION     DASYWM010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61647"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VGR29_05815"
     CDS             <1..255
                     /locus_tag="VGR29_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073143.1"
                     /translation="RDMQSQFDHQYLLQSRIDQYHRDAIRAEQLHIVGAGLPNRIIVA
                     TTKMNASMSTMLTVAHAYFRRHVTDSPEPGVETVHPRATA"
     gene            316..585
                     /locus_tag="VGR29_05820"
     CDS             316..585
                     /locus_tag="VGR29_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073144.1"
                     /translation="MHAQQYEHEHQAQVIITRYHHEADQVRKLRQARGEQPHRIVEAS
                     VRLRATVGAILIAAGQYLRQEPVVSPDQSPRRDVKAEPADSTVAV"
     gene            927..1697
                     /locus_tag="VGR29_05825"
     CDS             927..1697
                     /locus_tag="VGR29_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2073145.1"
                     /translation="MTRVIAFFNQKGGTAKTTSTLNVAAALAERGCRVLTIDLDPQAS
                     LSMAIGTDVTSLDLSVYDLLLDDEIGIEQVTHPTTITGIDIVPSHPDLAAAELELLNV
                     LERERQLDHRLHAADLSAYDYVLVDSPPALNIISINILVAIGELVVPIEPHPLSLMVL
                     KRLFETIRRISRLNPDLRVLGFLPTKVHHSSRLAQDMLVQLEEQFPELPLLPAIPLSV
                     RGAESTAERTSVLQYMPRSNVAAAYRDVGRWLEEAGDD"
     gene            1690..2697
                     /locus_tag="VGR29_05830"
     CDS             1690..2697
                     /locus_tag="VGR29_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV2073146.1"
                     /translation="MTERAGRSRRRFTVDALFSDTRPQATGVADLAEAKEIDLPRIEP
                     DPHQPRRTFDEERLEELASSIRTEGVLQPIVVRYEQERDIYVIVHGERRWRAAQRAGL
                     NVIPALVRDVPEERRLLQQLMENVVRDDLNAIDRAAALRTLREQLGGVPWEQVAETVG
                     IKRSRLFQLLGTEKLPDAAQEDIQTGRLSEKQSRALQGLPAIRQDALRQLIVSEKLPA
                     PKASRLAKAFRSLPIIHDDDPDAVMKALDDLYALVTPTAPEDLRAQTEAILDSVRNTN
                     SSDMAELIAAPKFDSDRVAKEVSALARSLSRVDRDDLASSPETESVLRALRDALNTLL
                     Q"
BASE COUNT          578 a          899 c          773 g          463 t
ORIGIN      
        1 cgcgacatgc aatcccagtt cgatcaccag tacctgttgc aatcccgtat cgatcagtac
       61 caccgtgacg ccatcagggc agagcagctg cacatcgtcg gcgctggact gccgaacagg
      121 atcatcgttg caacaacgaa gatgaacgcc tcaatgagca ccatgctgac ggtcgctcac
      181 gcctatttcc gtcgccacgt gactgacagc ccggaaccag gcgtcgaaac agtccacccg
      241 agagcaactg cctgatgcca tggacgacga aaacattacg tcgaaacgtg ttgccatcaa
      301 aaggagagac ccgttatgca cgcgcagcag tacgaacatg agcaccaggc tcaagtcatc
      361 attaccaggt accaccacga ggcggaccag gtcaggaaat tacgccaggc acggggggag
      421 cagccacacc gaatcgtgga agcctcggta cgcctccggg ccacagttgg cgcaatcctg
      481 atcgccgccg gccaatacct gcgccaggag ccggtagtga gcccggacca atcgccccga
      541 cgcgacgtga aggccgaacc ggctgacagc accgtggcag tctgatcggg cgatgcggca
      601 ccgaagccaa cggcaaaaca gtcagacgaa acctctactg cttcaacgat gtagatcatt
      661 gaagcagtag aggttttcct gtggcctccc ggctcaggag gtaatggaga ccgaactggc
      721 agccctgcct tgccatccca cgccgacacg atgacagggc tgaccgccgc tgttcacttc
      781 cacccgcttc gatgcggaga cgcacaccgc ccgaaagatg cacggcctcc cgcccgctcg
      841 gtatgatgga gtgagcatcg gtgccctcct tggcaccacc agggacccac cctggcttcg
      901 caccagagga tcgagcacaa ccccggatga cccgtgtcat tgccttcttc aatcaaaagg
      961 gtggcaccgc gaagacgaca agcaccctga atgtcgccgc tgccctggcg gagcgcggct
     1021 gccgcgtgct cactatcgac cttgacccgc aggctagcct ctcgatggcc atcggtacag
     1081 acgtcacctc gcttgatctc tcggtgtacg accttctcct cgacgacgag atcggcatcg
     1141 agcaggtgac ccacccaaca acgatcacag gcatcgatat cgtgccctcc catccggatc
     1201 tcgccgcggc agagcttgag ctgctcaacg tgctcgagcg tgaacgccaa ctggatcacc
     1261 ggctacatgc cgccgatctt tccgcctacg actatgtcct ggtcgactcc ccgccagcgt
     1321 tgaacatcat ctcgatcaat atcttggtcg cgatcggcga gctggtggtc ccgatcgagc
     1381 cacatccact ttccttgatg gtgctgaagc gcctcttcga gacgattcgc cgtatttcgc
     1441 gcctcaatcc agatctccgg gtactcggct tcctgccgac caaggtccat cactcgtcgc
     1501 gtctcgcgca agacatgctg gtgcaactcg aagagcaatt cccggaacta ccgttactcc
     1561 cggcaatccc gctctccgta cgaggagcag aatcaaccgc cgaacgtacc agcgttctgc
     1621 aatacatgcc gcggtcgaat gtcgccgcag cctacaggga tgttggccgc tggcttgagg
     1681 aggcagggga tgactgaacg cgccggccgg tcccgtcgcc ggttcaccgt tgatgcgctc
     1741 ttcagcgaca ctcgacctca agctaccggg gttgctgatc tcgcagaggc caaggagatc
     1801 gatcttcccc ggatcgagcc ggacccgcac cagccacggc ggacgttcga cgaggaacgt
     1861 ctggaagaac tggcgtcctc aatacgcacc gagggtgtgc tgcaaccgat cgtcgtgcgt
     1921 tacgaacagg agcgcgacat ctacgtcatc gttcatggcg aacgccgctg gcgggccgct
     1981 cagcgcgccg gactcaatgt gatccccgcg ctcgttcggg acgttcccga ggagcggcga
     2041 ctgctgcagc aactgatgga aaacgtcgtc cgcgacgacc tcaatgcgat cgatcgcgca
     2101 gcagccctac ggactctgcg ggagcaactg gggggcgttc cctgggagca ggtggctgag
     2161 acggtcggca tcaagcgcag ccggctcttc cagctcctcg gaacagaaaa gctccccgat
     2221 gcagcgcagg aggacatcca gactggccgc ctcagcgaga agcagtcgag ggcccttcag
     2281 ggactccctg ccatccggca ggatgcgcta cgccagctga ttgtgtcgga gaaactgccg
     2341 gctcccaagg cgagcaggct ggccaaggcc ttccgctccc ttccaatcat ccacgacgac
     2401 gatcctgatg ccgtgatgaa ggcactggac gatctctatg cgctcgtgac accgacggcg
     2461 ccggaggact tgcgagcgca gacggaggcg atcctcgact ctgtccggaa cacaaactcg
     2521 tctgacatgg cggagcttat cgctgcgccg aagttcgaca gcgacagagt cgcgaaggaa
     2581 gtttccgccc tcgcccgctc actcagccga gtggatcgcg acgacctcgc ttcctccccg
     2641 gaaaccgagt cggtgctccg ggcattgcgc gacgcactga atacgcttct ccagtaagac
     2701 ggaacagcgg cgg
//
LOCUS       DASYWM010000144        14027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_42567, whole genome shotgun sequence.
ACCESSION   DASYWM010000144 DASYWM010000000
VERSION     DASYWM010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14027
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42567"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="VGR29_05835"
     CDS             <1..1879
                     /locus_tag="VGR29_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEV2073147.1"
                     /translation="SPSIVDVMVNRERIGVLLTDFVNGGFAVPPASEHSLLRSFLEEL
                     REESGVDFTTYRQATIQRRVQRRMAATGEASLQDYVRLVRQHPEERRRLTSSFLINVT
                     HFFRDPELFDYLRDQILSELMHEARNRGEDLRIWSAGCSTGEEAYSLSIMVSELLAEK
                     EPGLNVRIFATDLDDEAVAFARRGFYSAHALSGLPPDMVSRYFIPHGDEYEVNKQLRG
                     MLVFGEHDLAQRAPFPRIDLILCRNVLIYFTPALQRRALQLFAFSMRKGGFLVLGKSE
                     SVSPLTEHFAVDQSRLKVFRRVGDRTAIPTTRVRDVMPPAASQHVTPRPAAPQQLMPP
                     TSSARAGSGEESLWNPERLLRVLPFGVVIVDRSYDIRDINGEARRLFGIHTTAMDQDL
                     IHLVQEFDPIQLRRLIDQAMQSPEGSRGLLVSTRTHGETQQSVEVLCTALSGPGRERD
                     GLIVVSAIDVTEREQIRHRRAAAEERSARLAKANEEVLASHQELTHLISRLRNENEQM
                     LIASAEIQAATEEVETLNEELQASNEELETLNEELQATVEELNTTNDDLQARTLELQT
                     LALRGRPRESSYAPSWTRSTTRSSWWTPAALSCSRTGCTAISLARAWDPQHSLMGRAP
                     F"
     gene            1774..2091
                     /locus_tag="VGR29_05840"
     CDS             1774..2091
                     /locus_tag="VGR29_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073148.1"
                     /translation="MDASGSIVLQNRMYSDLLGQGLGSPTFLDGKGSLLSDEQSPILR
                     AARGERFTVTFQVRSADGATARYEAMGHPVAMVSGPRLGVLTIRPLPDPDGREVAGEQ
                     SPA"
     gene            complement(2182..3087)
                     /gene="murB"
                     /locus_tag="VGR29_05845"
     CDS             complement(2182..3087)
                     /gene="murB"
                     /locus_tag="VGR29_05845"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV2073149.1"
                     /translation="MIAQLVLPNIQHNVVLAPFTTYKIGGPADAFVTVTSKDELVTAV
                     RRAREHDIPYFLLGTGANILIGDQGFRGLVIHNRAQHVSISGVSLTAGSGATIAELIA
                     LTAEKGLSGFEHFAGIPSSVGGAIWQNLHFLAPDRIETLFIEGIVESAEILDIRGDRR
                     TVNREFFEFGYDDSRLHHEEIIVLDVTFALSEKPTEEIMAQARANLEWRARRQPPVQE
                     FPSCGSVFKKIEGVGAGRLIDQVGLKGYQVGGIQISPKHANFLVNLGGGTARNVMDLV
                     RHAQQEVRRQTGYELEMEIGLVGEF"
     gene            complement(3211..4602)
                     /locus_tag="VGR29_05850"
     CDS             complement(3211..4602)
                     /locus_tag="VGR29_05850"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073150.1"
                     /translation="MSHAYSTRLFSRWRIIAVLTALLVTIASLATVVGQGFGPAGPSP
                     ATGHTSVVANGVVSLEEGSSRWHITRHIAEAGGSPIQITSPGFVLAENTPLLVTNQAS
                     GERYRLAAGEALMLHRGTEVTVETFGAPDSFIFVHLVPDEAEPLANATERILTSASFP
                     VQGGDYDADLLRDVLDEGETASIPAGALPTSLYVLRGEVGITSGNDERTLGAGESAIF
                     SGDLEVSAIADGSVFYAGFLGASVPVVDSPVTPVAATPVPATPEATALPATPPPAPPT
                     PEPVPPTPVPAADAAADSDGDGLTDAQEATIGTDPANPDTDEDGITDGDEINVWGSDP
                     LNLDTDGDLLYDGGELLFVTAILDPDTDDDFLSDGSEVYIYETDPTNPDTDGDGVRDG
                     AEIYNGTNPLAAPAQPAPPVASGDTDGDGLTNAQEARFGTNPADGDSDGDGVNDSNEV
                     AAGTNPLDRASFP"
     gene            complement(4847..5452)
                     /locus_tag="VGR29_05855"
     CDS             complement(4847..5452)
                     /locus_tag="VGR29_05855"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV2073151.1"
                     /translation="MAFTLPPLPYANDALEPTIDAQTMEIHHDRHHATYVTNLNAALE
                     GTEFADMDITELMKNLNSVPEEKRTAVRNNGGGHANHSFFWVLLTPGGSQQPTGDLAT
                     AIDQTFGSFDAFKEEFQKKTAAQFGSGWGWLVADGGNVSIVTTPNQDTPLSQGQIPLI
                     GVDVWEHAYYLKYQNKRPDYLKAVWNVINWDEAEKNYQSAR"
     gene            complement(5576..6058)
                     /locus_tag="VGR29_05860"
     CDS             complement(5576..6058)
                     /locus_tag="VGR29_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073152.1"
                     /translation="MRLPGTEKRSLRDQVTAGALAGAAASLAYAIEQEIDLRAFDHYA
                     DDLTLLGGLITDGPQARRSIGLVLHLFNGAAAGVLYATLLHHRIPGSPPMRGIMFGLA
                     ENTFLYPLALLEDKHPAIRKGTLSPYWNLSAFTQETLRHVVFGAVLGSAAERLLNRKG
                     "
     gene            6537..7595
                     /gene="aroF"
                     /locus_tag="VGR29_05865"
     CDS             6537..7595
                     /gene="aroF"
                     /locus_tag="VGR29_05865"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV2073153.1"
                     /translation="MLITVSAGSEHTKHEILRAAAGRGLMTQTMANGLGSEVIAIGGA
                     LPADAIALPGVERVQAAHKPYMLASLEARGTSHIRVGDITIGGGDPVIMAGPCSVEGR
                     EAQIEVARQVKAAGATMLRGGAFKPRTSPYSFQGLGELGLQYLAEAREETGLPVVTEV
                     MEPDQVELVAKYADMLQIGSRNMANFPLLKRVGVSGRPVLLKRGFSSTLEEFLMSAEY
                     VMAHGNPNVVLCERGIRTSDPAFRFNLDLNIVPGIKSVSHLPVIVDPSHGTGRRELVE
                     RMSLAGIAAGADGLAIEVHPDPDNALSDGYQTITPAALRRIHSRMMALHQVLLDTEDD
                     VAEITPIHAGDEMIAITA"
     gene            complement(8147..10072)
                     /locus_tag="VGR29_05870"
     CDS             complement(8147..10072)
                     /locus_tag="VGR29_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073154.1"
                     /translation="MDNHSTSRPDPDDEAIRDNTRYPESASNDTPSDLVTLPDEGVQA
                     PPLDVDERIGSTGQPEVIALPDNADGQQNAADHDFEDEIDEHMPLPVKEDYHDDDRVL
                     TDPHDVQDEVDEVTPLPAGDAWDHTDDFSTDEELDVVTAPSRRDPAETREAASPDNYM
                     TIDPESSEADAHPGSSDRVRFVDPSSTSPIGGTPVYMGGPSDNDPINNESNVYSSSYS
                     SSTTDISDEGADTSDEESLASTSTTGFLTPPLPPPRLDGITTCPVCGQSTNALRFCGY
                     CGAQLTENRHELTATSFRGHVEERAGMLLDPLARWTRPAPVRLIMLAGAALVLLALLA
                     NSGGLALIFAAMILPLILVFWCLHKDLFENEPLAIVAGFGVAGTLIGGVLGWLASLVV
                     TNSWIEGGTLNYGAAGYGGFLAERAGNPPFVVWTLVGIILPLLALTAIVGGPLAMRQT
                     ISLRNEVMDGLTLGALVGAGYSIGTAVVFASPMVTQGGPESDASSWTLTTIGLTIIRP
                     LIWTLSGGMLGAAAWRYLLTGTIRSALVPALVGSTVPLLFTLVSVQISPAGLWPEFLS
                     GLLMAAVAGFFFTKTIDQAIRHDRRILGNDGSRTVCPNCHQVTPAGHFCAHCGSDLTA
                     DRSSVTRMDDGDLQEAK"
     gene            10241..10921
                     /locus_tag="VGR29_05875"
     CDS             10241..10921
                     /locus_tag="VGR29_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEV2073155.1"
                     /translation="MSLHLYPRNNASVTPMETLGLIVYGMTVGVTLAAPIGPINVEII
                     RRGIRDGFVHGWVLGLGALSADTIYAALIVAGLTPLADRPALRVPLFLAGGLMLGWVG
                     WMSVRTALCGDTVDTGGRIPRRGRSYITGFLMAVFNPMGIVYWLSVGAALVADAVQRV
                     GQVGSPILVMGVMFGIFCWVTFVSMLAQVSRRFVTGSGMRWVTGISGVIIIGFALWFF
                     WQGFTTLI"
     gene            11029..13011
                     /locus_tag="VGR29_05880"
     CDS             11029..13011
                     /locus_tag="VGR29_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2073156.1"
                     /translation="MIYQPSPRQRPLEDITATRRRVVRGMGLGAAGIGLAAAGIRASG
                     QGATPDASPISSPESTPLPSAVPKTSLTIVRDQRPEYRGTPQPGGDLRLYIRREDLHR
                     FSPTAQRQDFQALISLLDPLVWIDSVTMEPQPWLASSWEWSSDGLMLTFQLRDDVLFH
                     DGSPLTAEDVRFSFIAYRDDYDSAMANFFALITDVAVDGPHTVRVVFAEPDGAFLFNG
                     ASQLIFSSAQYLPYWESMPVGERSLSDYDWASVAPLGTGPWQLAGMSDDSLLFGRFEA
                     YWNGSPHFDRLELIGEDDQQSRLEGWKDGDVDIVFPIRATEMDGLWDEEGSLFVAEAP
                     VAFFAAFNFANPANVTADMMGDPALRRALTLAVDRTGYAREIFFGFIDEMKAGTVTQP
                     WAHESLIKNPKQDIKEANRILDEGGWRDVDGDGMREDAVGNRLDLYCIVSNRERPELL
                     AILEGLKEDFSKIGARLTLERLDPDVLDDRWVQNRMYDMVAFSLIQYPAFAEYDLYGT
                     AWDIRVNRMGWNPGGYSNEAVDAAIQQYFGAWLQPQMKAALSMLQIAANEDLFGLWFG
                     FPHDLVLVRPDIQGFRPHKYFQTWNTRLLWRGEPFVEAPAPSPAATPVGSPGATPVVE
                     SDGTPGATPVASPVASPQATPLASPVASADRVHGRW"
     gene            complement(13880..14026)
                     /locus_tag="VGR29_05885"
                     /pseudo
     CDS             complement(13880..14026)
                     /locus_tag="VGR29_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         2627 a         4068 c         4531 g         2801 t
ORIGIN      
        1 gtctccgtcc atagtcgatg tcatggtgaa ccgcgagcgg atcggcgtcc tgttgaccga
       61 tttcgtcaac gggggctttg ccgtaccgcc ggcatcagag cattcgctgc tccgatcgtt
      121 cctggaggag ctgcgagagg agagcggcgt cgacttcaca acgtaccggc aggcaactat
      181 tcagcgacgg gtacaacgac gtatggcagc gacgggggag gcgtcgctcc aggactatgt
      241 gcgtttggtc cgtcagcatc cggaggagcg ccgccggctg acaagcagct tcctcatcaa
      301 cgtcacccac ttcttccgtg accccgagct ttttgattac ctccgcgatc agatactgtc
      361 ggaacttatg cacgaggcca gaaatcgcgg agaggacctg cgaatctggt ccgccggttg
      421 ctcgacgggg gaagaggcct attccctgtc aatcatggtc tccgagttac tggcggagaa
      481 ggagccaggc ctcaatgtcc gaatcttcgc caccgatttg gacgacgaag ctgttgcgtt
      541 tgccaggcgg ggattctact ccgctcatgc gttgagtggt ctgccccccg atatggtgtc
      601 gcgatacttc attcctcacg gtgatgaata tgaggtgaac aagcaacttc gcggcatgct
      661 cgtctttggt gagcacgacc tggctcagcg ggcgccgttt ccgcggatcg acctgattct
      721 gtgccgtaat gtgctcatct actttactcc cgccttgcag cgacgtgcgt tgcaactctt
      781 tgcgttctca atgcgtaagg gaggcttcct cgtattgggg aaatcggaga gcgtgagccc
      841 gctgacagag cactttgccg tggaccaatc ccggctgaag gtcttccgtc gggttggcga
      901 ccgaacggca atcccgacga ctcgggtccg ggacgtcatg cccccggcgg cgagccagca
      961 tgtcacgcct cgccctgcgg caccgcagca attgatgccg ccaacctcat ctgcaagagc
     1021 tggcagcgga gaagagtcat tgtggaatcc ggagcggctc cttcgtgtgc ttccgtttgg
     1081 cgtggtgatt gtcgatcgaa gctacgacat ccgcgacatc aatggtgagg cgaggagatt
     1141 atttggcata cacacgacgg cgatggacca ggatctgatc catcttgtgc aggaattcga
     1201 tcccatacag ttgcgccgcc tcatcgacca ggccatgcag tcaccagagg ggtcacgggg
     1261 tctgctggtt tctacccgaa cgcatggtga aacccaacag agcgtggaag tcctgtgcac
     1321 ggcgctgagc ggcccgggca gggagcgaga cgggttgatt gtggtctcgg ctattgacgt
     1381 gaccgaacgc gagcagattc ggcaccgtcg ggccgccgcg gaagagagat ccgcgcgcct
     1441 ggcaaaggcc aacgaagagg tgctcgcttc gcatcaggag ctcacccacc tgatctcccg
     1501 actccggaac gagaacgagc agatgctgat agcgagcgcg gagatccagg cggcgactga
     1561 agaggtcgag accctcaacg aagagctcca ggcgagcaat gaggagctcg agaccttgaa
     1621 cgaagagttg caggcgaccg tcgaggagct caacacaacg aacgacgacc tgcaggcgag
     1681 gactcttgag ctgcaaaccc tggcccttag ggggagacct cgcgagagca gctacgcgcc
     1741 atcctggacg cggtcgacga cgcgatcgtc gtggtggacg ccagcggctc tatcgtgctc
     1801 cagaaccgga tgtacagcga tctccttggc cagggcctgg gatccccaac attccttgat
     1861 gggaagggct cccttctaag cgatgagcag tcgccgatcc ttcgagccgc caggggagaa
     1921 cgattcaccg tgacgttcca ggtacgttcg gctgacggag ctaccgcccg ttacgaggcg
     1981 atggggcacc cggtggcgat ggtcagtggc cctcgtctgg gcgttctgac aatccggccg
     2041 cttcccgatc cggacggccg ggaagtcgca ggggagcaat cgccagcctg agggttcctg
     2101 acgtgtatgt tcaccgagag ccggatgctg gtgcattcga gcgatggccg ccaatagtca
     2161 tggaccatag gccaagggga tctaaaattc gccgacgagc ccaatctcca tctccagctc
     2221 gtagccggtc tggcgccgta cctcctgttg ggcgtgacgt accaagtcca tcacattccg
     2281 cgcggtgcct ccaccgaggt tcacgaggaa gttggcgtgt ttcggggaaa tctggatacc
     2341 acccacctga taacccttga gccccacctg gtcgatcagg cggcccgccc cgaccccctc
     2401 gattttcttg aaaacggagc cgcaggaggg aaactcttgg accggaggct gtcgccgggc
     2461 gcgccactca aggttcgcgc gagcttgcgc catgatctct tctgtaggtt tttcggagag
     2521 cgcaaaggtg acgtccagca cgatgatctc ttcgtgatga aggcgactgt cgtcgtagcc
     2581 gaactcgaaa aactcccggt tcacagtgcg ccggtccccg cgaatgtcga ggatctcagc
     2641 cgactccacg atgccctcga taaagagcgt ctcgatccgg tccggcgcaa gaaaatggag
     2701 gttctgccag attgcgccac ccacactgct agggatgcct gcgaaatgct cgaaaccgga
     2761 gagacccttt tcggcggtca gggcaatcag ctcggcgatg gtggcaccgc tcccggcagt
     2821 gagggagacg ccggatatgg agacgtgttg ggcgcgattg tggatgacga gacctcggaa
     2881 accctggtcg ccaatgagga tattggcgcc agtgccgagg aggaaatagg ggatgtcatg
     2941 ttcccgggct ctgcgtaccg ctgtgaccag ctcatccttt gaggtcactg tcacgaacgc
     3001 gtccgctggt cccccgatct tgtaggtagt gaagggagcc aggacgacat tatgctggat
     3061 atttggcagg accaattggg cgatcatcgg gtgactctac tccggcgcgg tgatgggtat
     3121 ccgcggctgg gccgatcctc ggtcatccct ggtgcgaccc agccgcaggg cttttagacg
     3181 acatataagc tgccgttcct gaaggagccg ctacggaaag ctggccctgt ccaggggatt
     3241 tgtgccggcg gcaacctcgt tcgagtcgtt tacaccatca ccatcgctgt ctccatcggc
     3301 cgggttggtg ccgaaccggg cctcctgtgc attggtcagc ccatctccgt cagtgtcgcc
     3361 ggatgcaact ggtggtgcgg gctgtgccgg ggcagccaga ggattggtgc cgttgtaaat
     3421 ctcggccccg tcccggacac cgtcaccgtc cgtatcggga tttgtcgggt cggtctcgta
     3481 gatgtagact tccgagccgt cgctgagaaa atcatcgtcg gtgtctgggt cgagtattgc
     3541 cgtcacgaag agcaactctc cgccgtcata gagtagatcc ccgtccgtat cgaggttaag
     3601 cgggtcagag ccccagacgt tgatctcgtc cccatccgtg atgccatctt catccgtatc
     3661 cgggttggcg ggatccgtac caattgttgc ttcctgcgcg tcggtcagcc catcgccgtc
     3721 agaatccgca gctgcgtcag cggccggcac tggcgtggga gggacaggct ctggagtcgg
     3781 aggcgcaggg ggtggggtag ccggcagggc agtagcctcg ggcgtggcgg gtactggcgt
     3841 cgctgccacg ggcgtgactg ggctgtcgac tacagggaca gatgcgccga gaaacccggc
     3901 gtagaagacg ctgccgtcgg cgatggcgct gacctcaaga tcgccagaaa aaatggcgga
     3961 ttcgcctgcg ccgagcgtgc gctcgtcatt cccgctggtt atgcctacct cgccgcgaag
     4021 gacgtagaga ctcgtcggga gagcacccgc gggaatggaa gctgtctcac cttcatcaag
     4081 tacgtcccgg agcaggtcag cgtcatagtc gccaccctga accgggaagg atgcgctggt
     4141 gaggatgcgc tcggtagcgt ttgccagcgg ctctgcctcg tcgggaacaa gatggacaaa
     4201 gatgaagctg tccggtgcgc cgaacgtctc caccgtcacc tctgtgccgc ggtgaagcat
     4261 gagggcctcg ccggcggcaa gccgatagcg ctcacccgac gcctggtttg tgaccaggag
     4321 cggcgtgttc tccgcgagca cgaaacccgg tgaggtgatc tggatcgggc tgccaccagc
     4381 ttccgcgata tggcgggtga tgtgccagcg actgcttcct tcctccaggg agacgacccc
     4441 gttcgccacc acggaggtat gacctgttgc cggagatggg ccagcggggc cgaaaccctg
     4501 tccgacaact gtcgcgagcg atgcgatagt gaccaggagg gcggtcagca ccgcgattat
     4561 cctccagcgg gaaaagaggc gggttgaata tgcgtgcgac atggcagggt ccttcgtctt
     4621 tgtgagtgca taggtcatct cgcccctgat gataccgcca tcgcaccgtc cggcgccgag
     4681 ggagggatgt tcccggcgac ctgcgtccta tgcatccgtg ggtgtttccg atatttgaac
     4741 caattggcag gggttcgatg actgtgcagt tccggctacc acaaagccgc ggccactatg
     4801 gtgaccgcgg ctttatccat gtggtgctgg cgttgttagg tgagcgttag cgggcgctct
     4861 ggtagttctt ttccgcctcg tcccagttaa tcacgttcca gactgccttg aggtagtccg
     4921 ggcgcttgtt ctggtacttc aggtagtagg catgctccca gacgtcgacg ccgatcaacg
     4981 ggatttgtcc ctggctgagc ggagtgtcct ggttcggcgt ggtgacgatc gagacgttac
     5041 ctccgtcggc gacgagccaa ccccaacctg agccgaactg cgcagcggtc ttcttctgga
     5101 actcctcctt gaaggcatcg aaggaaccaa aggtctggtc gatcgccgtg gcgagatcac
     5161 cagttggttg ctgcgaacca ccgggcgtca gcagtaccca gaaaaagctg tgattggcat
     5221 gtccgccgcc gttattccgg acagcggttc gcttctcttc gggcacggag ttgaggttct
     5281 tcatcagttc cgtgatgtcc atatcggcga actcagtgcc ttcgagcgcc gcattgagat
     5341 tggtcacata ggtggcgtga tgcctgtcgt gatgaatttc catcgtctgg gcatcgatgg
     5401 tcggctcaag agcatcgtta gcgtaaggaa gcgggggaag tgtgaaagcc atgtggcatt
     5461 tctcctgttc tcatcaatcg actctacgag acggcacacc ggtttttccg gcgctctttc
     5521 cgtcttcggc caatgcgtat tgtctagcaa gtcgtgggcc aagggagtgc caaaatcagc
     5581 ccttccggtt gagcaggcgc tcggcagccg atcccagcac tgcgccgaag acgacgtgac
     5641 gaagtgtctc ctgggtgaac gcagacaggt tccagtacgg ggacagggtg ccttttcgga
     5701 tggccgggtg cttgtcctca agcagcgcca gggggtaaag gaacgtgttc tccgccagac
     5761 cgaacatgat gccgcgcatc gggggtgagc cggggatgcg atggtgcaac agcgttgcgt
     5821 atagaacgcc ggccgcagcg ccgttgaaga gatgcaggac gagaccaatc gagcgtctcg
     5881 cctgtgggcc gtcggtgatg agccctccaa gtaacgtgag gtcgtcggca taatgatcga
     5941 aggcccggag atcgatctct tgctcgatgg cgtaggcaag cgaggccgcc gcaccggcga
     6001 gggcgcctgc ggtcacctgg tcccgtaacg aacgcttttc agttcccggt agtctcatgt
     6061 gtgttccttg cgtgagggac tcgacgttca tctttggcaa tgttgcccag ccatgcgatt
     6121 cgtgtgccat cgggatcggg gccttgcggg gcagccgtcc aaaaagtagg tcggcgtggc
     6181 gcctattgcg catgcgaaaa gggttgacat ctggaaacct ggtcggttac cctgcatcca
     6241 tcatgacgag caaccgcctg aacccaccag tcgaagagac agagatgccc atggccgcag
     6301 caagcggcac atgcgcatta acgtcctcgg tttggtatta ctggtttacg aaccccccgg
     6361 ctgtgctggc ggggcgattt gagtgtaccg actagggaca caagagacgc caccgagcaa
     6421 ccagacgcag cactgagggg ggaaccgaga cagacgtctc ggttttttgt tgcccgatga
     6481 tgaccgagtc ggacatgtta gccactggaa cgtacgagat cagagaggaa cgagccatgt
     6541 tgatcaccgt atccgccgga tccgaacaca ccaagcacga gattctgcgg gcggccgcag
     6601 ggcgcgggct gatgacgcag accatggcca acggtcttgg tagcgaggtg attgccattg
     6661 ggggggcgct tcctgcagat gcgattgccc tccctggcgt ggagcgggtg caggcggccc
     6721 acaagcccta catgctggcc agcctcgaag ctcgtggcac cagccacatt cgtgtcgggg
     6781 atatcacaat cggcggtggt gatccggtca tcatggcagg tccatgctcg gtagaagggc
     6841 gcgaggccca gatcgaggtg gcccgtcagg tcaaggccgc tggcgccacg atgcttcggg
     6901 gtggcgcctt caagcccagg acgtccccat actcgttcca gggactgggt gagctgggat
     6961 tgcagtacct ggccgaggcg cgcgaggaaa ccggtctgcc ggtggtaacc gaggttatgg
     7021 agccggatca ggtggagctt gtggcgaagt acgccgacat gctgcagatc ggctcccgca
     7081 acatggccaa cttcccgctg ctgaagcggg tcggagtgtc cggcaggcct gtcctgctca
     7141 agcgcggctt ctcttcgaca cttgaagagt tcctgatgag cgctgagtac gtgatggcgc
     7201 atggcaaccc gaatgtcgtg ctctgtgagc gcggcatccg tacctcggat ccggcgttcc
     7261 gcttcaacct cgatctcaat attgtgccgg gaataaagag tgtttcgcat ctgccggtca
     7321 tcgtggatcc aagccatgga acaggtcgcc gggagctcgt cgagcgaatg tccctggccg
     7381 gtatcgccgc aggcgcggac ggtctcgcaa ttgaggtcca tccggacccg gacaatgccc
     7441 tgagcgatgg ataccagacg atcacgcccg cggcacttcg ccgtatccac agccggatga
     7501 tggcactgca tcaggtcctg cttgatacgg aggacgatgt cgccgagatc acccctattc
     7561 atgcaggcga tgagatgatc gcgatcactg cgtagtcctt gcagcaggag catggtgcgg
     7621 gactgcacgg gagaggcccg caaactggtg gcttttgggc ggtatgagcc ccacttggcg
     7681 ttccgtgttc gtacatctgt tgatttcctg catttgctcc ttgacaacta ggagtaacgc
     7741 ggttaacgtg aagaggctga ggcatcggac caactgccgg acggtccggg atatcggaat
     7801 ataccgatcc acctgagatc gaccggcgct gtgggtggct ggaagcgcgg ttttccgcga
     7861 taccatttcc agatctctca ggaactcggt cagacgccat cttcggcggc tgatttctca
     7921 gacaattgag cggggaccgc accacctccc tggtcagatg gctgagacct cgcacacgcg
     7981 aggattacgg cgaggttctg gttgttcacc ctacaaggtg accgaccagc ctgggacgct
     8041 gactttccca aggcagtgac tctgatgagg tgcttcggaa tagattggaa tgccggcccc
     8101 cgcaaggggg tcggcgtttg tctgtacgtc cgaatgtacg gctggatcat ttcgcctctt
     8161 gcagatcccc gtcgtccatc cgcgtgaccg aagacctatc ggcggtgagg tcggacccgc
     8221 aatgggcgca gaagtgtccc gccggtgtca cctgatggca gttgggacag accgtcctgg
     8281 agccatcgtt gccgagtatc cggcggtcat gccgtattgc ctggtctatg gttttggtga
     8341 agaagaaacc cgccacggcc gccatcagga gaccgctcag gaactccggc cagagacctg
     8401 ccggagagat ctggaccgat acgagggtga acagcaatgg aactgtggac ccgaccaggg
     8461 cgggaactag cgccgagcgg atcgtgcccg tcaacaggta ccgccaggcg gccgcgccca
     8521 gcatgccgcc actgagtgtc cagatcagcg gacgaatgat ggtgagcccg atcgttgtca
     8581 gcgtccagct ggaggcatcc gactccggtc caccctgggt caccatgggg gatgcgaaga
     8641 cgacggccgt gccaatcgaa tagcctgctc ccacgagtgc gcctagcgtg agaccgtcca
     8701 tcacctcatt tcgcagagag atcgtttgcc gcatggcaag gggtccccca acgatcgcgg
     8761 taagcgcaag caatgggagg atgatgccca ccagggtcca cacgacaaac ggggggttcc
     8821 ctgcccgttc ggccaaaaaa cctccatacc cggctgcgcc gtagttgagg gttccccctt
     8881 cgatccagga gttggtcacg acaagcgatg cgagccaacc gagcaccccg ccgatgagcg
     8941 ttcccgccac accgaagccg gcaacgatag ccagtggctc attctcaaag agatctttgt
     9001 ggaggcacca gaaaaccagg atgagcggca ggatcatggc ggcgaagatg agggcgagac
     9061 cgccgctgtt cgccagcagg gcaagcagga cgagcgcagc accggccaac atgatgaggc
     9121 gtacgggagc ggggcgggtc cagcgggcga gcggatcgag tagcatcccg gcgcgctcct
     9181 cgacgtgtcc cctgaaggat gtcgcggtga gctcgtggcg gttctcagtc agctgcgcgc
     9241 cacaatagcc gcaaaagcgc aaggcattcg tcgactgccc gcagacaggg caggtagtaa
     9301 tgccgtcaag ccggggaggt ggcaggggag gggtgaggaa acccgtcgtg ctggtggaag
     9361 cgagtgactc ctcatcgctt gtgtcggcac cctcgtcgct gatatccgtt gtgctcgatg
     9421 aatatgacga cgagtagacg tttgattcgt tgttgatcgg atcgttatcg ctcgggcccc
     9481 ccatgtagac tggcgtgcca ccgatggggg aggtactcga cggatctacg aaccgaaccc
     9541 ggtccgatga gcctggatgg gcgtcggcct ccgaggattc cggatcgatg gtcatgtagt
     9601 tgtcaggcga agccgcttct cgcgtctcag ctggatcacg cctggaaggt gccgtgacga
     9661 catcgagttc ctcatccgtt gagaaatcgt cggtgtgatc ccaggcgtct cctgccggaa
     9721 ggggcgtcac ctcatcaact tcgtcctgga cgtcatgcgg atcggtgaga acacggtcgt
     9781 cgtcgtgata atcctccttg acgggcaagg gcatgtgctc gtcgatctcg tcctcgaagt
     9841 catggtcggc tgcgttctgc tgaccgtcgg cattgtccgg caaagcgatg acctccggct
     9901 gcccggtgga gccaatccgc tcgtcaacgt ccaggggagg agcttgcact ccttcgtccg
     9961 ggagggtcac cagatcagac ggtgtgtcat tggatgcgga ctctggatac ctggtgttgt
    10021 cgcggattgc ttcatcatcg ggatcgggac gcgaggttga atggttatcc atgcatgctc
    10081 cttgctccgt cgggaagcct tggcaggact ggcggcgtcc catggcccgg gctggcaggc
    10141 cgtctatcga tcgggtggcg ctgtgctgtc gttccggata gtttgccacg ctccactgaa
    10201 tcgcgctctg atagactccc gggcaggagt tcgccgtctc gtgtcacttc atctttatcc
    10261 aaggaacaat gcctcagtga ccccgatgga aacgcttgga cttatcgtct atggcatgac
    10321 ggtcggtgtg acccttgccg cgccgatcgg tccaatcaat gtggagatca tccgccgggg
    10381 catccgtgac ggctttgttc atgggtgggt gctgggcctg ggggcgctca gcgcagacac
    10441 gatctatgcc gcattgatcg ttgcgggtct gacaccgctg gccgatcgtc cagcgttgcg
    10501 ggttccgctc tttctcgcgg gcggtctcat gctgggctgg gtcggttgga tgagcgtccg
    10561 aaccgcgttg tgcggggata ctgtcgacac cggcggtcgt attcccaggc gcgggcgcag
    10621 ctatatcacc gggtttctga tggccgtctt caacccgatg gggatcgtct actggctctc
    10681 cgttggcgcg gcacttgtgg ccgatgcggt ccagagggta ggacaggtgg ggtcaccgat
    10741 cctggtcatg ggggtgatgt tcggcatctt ttgctgggta acgttcgtct ccatgctcgc
    10801 ccaggtctcc cgccggttcg tgacggggtc cggcatgcga tgggtgaccg gcatcagtgg
    10861 tgtcatcatc atcgggttcg cgctctggtt cttctggcag ggtttcacga cactaatcta
    10921 ggtcttccgc aagcagggac tcatcgaaac gaatgtacgt acagatgtac aatggtgcct
    10981 gacgtttgga cggcccatgg tcggatactg gaatcaagca cagatcatat gatctatcag
    11041 ccttccccta gacagcggcc cctggaagat attacagcga cgcgacggcg cgtggtgcgt
    11101 gggatgggtt tgggggctgc cgggattggg ctggccgccg ccgggatccg tgcctcaggt
    11161 cagggggcaa ccccggatgc ctctccaatc tcgtcgcccg agagcacacc cctcccgtcc
    11221 gccgtgccga agacctctct tacgatcgtg cgcgatcagc gacccgagta tcgcggtacg
    11281 ccacaacctg gcggcgatct tcgtctgtat atccggcgtg aggacctcca ccgcttcagc
    11341 cccaccgcac agcggcagga cttccaggca ctcatcagct tgcttgatcc cctggtgtgg
    11401 atcgactccg tgacgatgga gccgcagccc tggctcgcgt catcgtggga gtggagcagc
    11461 gatggcctga tgctgacgtt ccagctccgc gatgatgtgc tcttccacga tggatcacct
    11521 ctcacggcgg aggatgtccg gttctcgttc atcgcatacc gtgatgatta cgacagcgcg
    11581 atggcgaatt tctttgccct aatcacggac gtcgctgtcg acggcccgca taccgtgcgg
    11641 gtggtctttg ccgagcccga tggtgccttt cttttcaacg gcgctagcca gctcatattt
    11701 tcaagcgcgc agtaccttcc gtactgggag agcatgccgg tcggggagcg atcgctctcc
    11761 gattacgact gggcgagcgt cgcgccgctc ggcacgggcc cgtggcagct cgcgggcatg
    11821 agcgacgact ccctcctgtt tggccgcttc gaggcctatt ggaacgggtc tccccatttc
    11881 gatcggctgg agctgatcgg cgaggacgat cagcagtcgc ggctcgaggg ctggaaggac
    11941 ggcgatgtcg atatcgtctt tcccatccgg gcgacggaga tggatgggct ctgggacgag
    12001 gaggggagcc tctttgtggc cgaggctccc gtcgccttct tcgccgcgtt caacttcgcc
    12061 aacccggcca atgtgacggc ggatatgatg ggcgatcctg cgttgcgccg ggcactgacc
    12121 ctcgccgtgg accggaccgg ttacgcgcga gaaatcttct tcggcttcat cgatgagatg
    12181 aaagccggga cggtcaccca accctgggcc cacgaatctt tgatcaagaa cccgaagcag
    12241 gacatcaagg aggccaatcg gatcctggac gagggaggtt ggcgcgatgt cgacggcgat
    12301 ggcatgcgcg aggatgcggt cggcaacagg ctcgatctct actgcattgt ctccaacagg
    12361 gaacggccgg agctcctcgc aatcctggag ggcctgaaag aggatttctc caaaatcggc
    12421 gcccgcctga ccctggagcg gcttgatcct gatgtgctcg acgatcgctg ggtgcagaac
    12481 cgcatgtacg acatggtggc gttctccctg atccagtacc cggcatttgc cgaatatgat
    12541 ctctacggca ccgcgtggga tatccgcgtg aaccgcatgg gttggaaccc cggtggatat
    12601 agcaacgagg ccgttgatgc cgcgatccag cagtacttcg gtgcatggct ccagccgcag
    12661 atgaaggcgg cgctcagcat gcttcagatt gccgcgaacg aggatctctt cggactctgg
    12721 ttcggcttcc cgcacgacct ggtgctggtc cgtcccgaca tccagggctt tcggccgcac
    12781 aagtacttcc agacgtggaa tacgcgtctg ctatggcgag gtgaaccatt tgtggaggca
    12841 ccggcaccgt ctccagcagc tacaccagtt ggatcgcccg gtgcgacgcc tgtcgtggaa
    12901 tcggatggaa cgcccggtgc gactccggtg gcatcaccgg tcgcctcgcc gcaggcaacg
    12961 cccttggcgt ccccagtggc ctctgctgat cgcgtacacg gccgctggta ggctggacgg
    13021 cgagcgaaat ccgggggccg gacgttggtt gggcgataac cgctccgctg gcatcctgga
    13081 cgggcagaga ccaagtaaaa gttccccacg ttgggtgttg gaaacatttc acgcatgccc
    13141 tgtagggctg tgcctgaacc ggtttcttgg caagggcggt gtgcaagagg tgggggtatt
    13201 gccagtgcct tagagccacc ggggttgcct ccattgcagg agtggcaggt cgggtcttgg
    13261 cggttgcgac gaaggcgctg accaaggcat ccagcccctt gggactgaac gcggcagcgg
    13321 tgaagagcca tggactggtg acgtcacccc acgcaagggt atagatcggt tgaggctcgt
    13381 tgccggcaat gccggcagta gtctcggcac ggacccaggc aagcgtgcca tcctggccaa
    13441 acgccacggc atcgtagtcg tatgcaggat tggtggcaat ggcaacgaag tcggcaacgg
    13501 tgaaatccat cgggaagaag tcgccagccg ctaaattgat ggcctgaagc atgagcggcg
    13561 gaccaaagtc gggatcggtc gtcccgtagc cggcaacgaa gcggccagcc tcctcgtagc
    13621 ccggagccga ggcttgcggt tcatccgcca cggcggcggg gagctggtcg aaaagttcgg
    13681 taatgccctc gatgtcgtcg ggaagcatga cggtccccaa cccaaatgga gccgtggccg
    13741 ccggcaacgc aggtgtcgct tccggggtgg cgtccggagc gcgacccccg gtctcagatg
    13801 ctggcgcgca ggttccgtcg ttgccactgt tcgagccatc gggacaggtg gtgttgaacc
    13861 agaaggcgcc ggtcagggtg gcgcccgtca ggtccgtacc ggtcaggtcc gcgtaggaaa
    13921 agtcggtttc ggcgaggctg gcgtcggtga gattggcgtg attgaaactg gcaccgttca
    13981 gcgtggcgcg ggttaagttc gcaccctcca ggttggcacc cgacagg
//
LOCUS       DASYWM010000145         5000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_5810, whole genome shotgun sequence.
ACCESSION   DASYWM010000145 DASYWM010000000
VERSION     DASYWM010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5810"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VGR29_05890"
     CDS             <1..714
                     /locus_tag="VGR29_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2073157.1"
                     /translation="EISRSPFALPTELRWENIERAWTVGGFDRYVLNTVIYCAAIVTG
                     VVALSCFSGYALALLPLPGRGVILTLFLLGLMVPFQSVMIPIYYLLRDIGLLETYWAF
                     IVPGIALRLPFGIFLMRGFFKGLPVDIADAARMDGANEWQVFRSIMLPLAIPGLSTLV
                     VFQFIYTWNQFLMPLIFVQRDALRPVALGTMFFFGRFTADRGMIAAGVTISMIPMVIL
                     YLLLQRRFIEGITAGAMKT"
     gene            819..2264
                     /locus_tag="VGR29_05895"
     CDS             819..2264
                     /locus_tag="VGR29_05895"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073158.1"
                     /translation="MTVDKNISGASPAEVGIGSSAKGLPAVNNGASADRHKGNDSFNT
                     TPSIRRTGSLRWPTTRWLRDVQLPHALQVAPFRNYWLSQIVAWAGTWMQQVGIQLVVL
                     SLTTSALAIGMINIVAAVPMLLLSLTGGVIADRFNRRHILMVSMGLLGLFSLVYAWLI
                     GTGSIEYWHILVFASLGGVVMSFQLPAAQAFVAELVPRDDLPQAIALNSASFNSTRIV
                     GPALAASAIGALGLASAFIMNAIALLAPIGTLFRLRKVLKTPVRARDSGSQFDALKLG
                     LRYVKSDDGTMGLMLLQAVISFFVTPPLLVLLPLYVVDDLGGAASWVGIMLSFMGAGS
                     LVGSLVLLRGSRLEAVAAKRLKVAVTGLAVGMAWMALSQDPVMAVPGVMLGGFSFAMG
                     NSQIMTRLQQTSPEELRGRIMSLISLAFNGVMPFSTLSLSALAQLIGLDIVIGISAVL
                     LAFCGVYLWRRFVWKAFIPEVMPGSGAQHSL"
     gene            2494..3225
                     /locus_tag="VGR29_05900"
     CDS             2494..3225
                     /locus_tag="VGR29_05900"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEV2073159.1"
                     /translation="MFFHVKELQFEAKLDRPGPLYAKRLQELLGGKFGEMTVMMSYLF
                     QGWNSRGPEKYRDMLLDIGTEEIAHVEMLSVMISRLLDKAPVKEQEDAAKIPVIGVAL
                     GGGNPSDVIFAAMNPQHEIVTGQGANAHDSVGVPWSGAYATASGNLMADFRWSLMAES
                     QGNLQVSRLYEMAEDQDIRKMLSFNLARDIMHQNQWQAAIEEIQTDGLHQFQVPSPFP
                     LDRVNLSQAYTFWNCSKGTASEEGR"
     gene            3262..3447
                     /locus_tag="VGR29_05905"
     CDS             3262..3447
                     /locus_tag="VGR29_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073160.1"
                     /translation="MYLADSAPLTDDIGELSQLDPRLHGKAKIPMPLMSSGPNPVPDQ
                     DIPKNVNKNAVTGDGKM"
     gene            complement(3722..>5000)
                     /locus_tag="VGR29_05910"
     CDS             complement(3722..>5000)
                     /locus_tag="VGR29_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2073161.1"
                     /translation="GPVVYGPPLPLALPTPIYITPRTRLILLLLIGAVFYLIATRASD
                     VVLLLFMGAIVALVLSFPVRFLNAFLPRRVAITIVAFSTLLFAIVLLALLIPFAINEI
                     SRFAESFPATVDDLQGLTRDLLFQFYDRGWMEQHPDEVLSDIEAGIFDAAQRMVTDLL
                     TNTVGWMTRSVNLLITTFGVIFVSIYLVADIPRFRDSFVRMWAPAYRNDAVVLWDTLG
                     YSLSRYLSGLLASILIQGVAAFIGLTVLGVPYALLLGIWTSMTAILPYIGAFLGAIPA
                     VAIALTISWQLALLTTLLFIVINQLEANFITPRIQGSAVRVHPLLIFFSVIAGSSMFG
                     GIGAVMAVPILAVLRVVGEFFWLRLRVREDGPTLLAAMRSDVAEERLSQQPPIPEIVE
                     EEAEKEIVQSAREGRTLSLDQVRDPEATTSPTP"
BASE COUNT          932 a         1502 c         1570 g          996 t
ORIGIN      
        1 gagatctcgc gcagcccctt cgcgttgcca acggagctgc ggtgggagaa catcgagcga
       61 gcctggacgg tcggtggctt tgaccgctac gtgctcaaca ctgtcatcta ttgtgcggcg
      121 atcgtcaccg gggtcgtggc cttgtcctgt ttctcggggt acgcgctcgc gctgctgccg
      181 ttacccggca ggggcgtaat cctgacgctg ttcctgctgg gactgatggt gcccttccag
      241 tccgtgatga tcccgatcta ctatctcctg cgggatattg ggctcctgga gacctactgg
      301 gcgttcattg tgccaggcat cgccttgcgg ttaccgttcg gcattttcct gatgcggggc
      361 ttcttcaagg ggttacctgt ggatattgcc gatgcggcgc ggatggacgg ggcaaacgag
      421 tggcaagtgt tccggagcat catgctgccg ctagcgattc ccggcctttc cacactggtt
      481 gtcttccagt tcatctatac gtggaaccag ttcctgatgc cgttgatctt cgtacagcgt
      541 gatgcgctac ggccggtcgc actcgggacc atgttcttct tcgggcgatt taccgccgac
      601 cgtggcatga tcgcagccgg agtgacgatc tccatgatcc cgatggtcat tctctacctg
      661 ctgctgcagc gacgctttat cgagggtatt acagcaggtg ccatgaagac ctaatcctcg
      721 gtgcaaatgt ctcctcaaca cggttgcccg gtgtgcgctc tcggcgataa tgcacatcag
      781 gcaaccgtgg cgatcgcgcc gcgaggggac gacacacgat gaccgtagac aagaatattt
      841 cgggtgcttc gccagcggag gtgggaatcg gctcctcggc gaagggtctt cccgccgtca
      901 acaacggtgc atcggctgat cgccacaagg gcaatgactc cttcaacact accccttcaa
      961 tccggcggac ggggtcgcta cgctggccga cgacgcggtg gctgcgcgat gtccaactcc
     1021 ctcacgccct gcaggtcgcg cccttccgga attactggct cagccagatc gtggcgtggg
     1081 ccgggacctg gatgcagcag gtcggaatcc agctcgtggt gctctccttg acgacctcgg
     1141 cactcgctat cggcatgatc aatatcgtgg cggccgtgcc gatgctgctg ctcagcctga
     1201 ccggcggagt gatcgccgat cggttcaatc ggcggcatat cctgatggtc tcaatgggcc
     1261 tgctgggtct cttctcgctt gtctatgcct ggttgatcgg gaccggcagc atcgagtact
     1321 ggcatatcct cgtctttgcc tccctgggcg gggttgtcat gtcattccaa ctcccggcag
     1381 cccaggcatt tgttgctgag ctggtcccgc gagacgattt gccccaggca atcgccctca
     1441 actcggcatc attcaactcc acgcgtatcg ttgggccggc gctggccgca tcggcgatag
     1501 gcgcgcttgg tctggcgagc gcctttatca tgaacgctat cgcgttactg gccccgatcg
     1561 gcacgctctt ccggctgcgt aaggtgctga agactcctgt tcgggcgagg gactccggat
     1621 cgcagtttga tgcgctcaaa cttggcctcc ggtacgtcaa aagtgacgat ggcaccatgg
     1681 ggctgatgct cttgcaggcg gttatctcgt tcttcgtcac cccgcccctg ctggtcctgt
     1741 tgccgctcta tgtcgtcgac gatctggggg gcgccgccag ctgggttggc atcatgcttt
     1801 cgttcatggg agccggctcg ctggtcggtt cactcgtgtt gctgcggggg agccgtctgg
     1861 aagcagtggc tgccaagcgt ctcaaggttg ccgtgaccgg gctggcggtg gggatggcgt
     1921 ggatggcgct ctcgcaggat cccgtcatgg cagtccccgg cgtcatgctc ggtggattct
     1981 ccttcgccat gggcaattcc cagatcatga cccggctcca gcagacatcc ccggaggagc
     2041 tgcggggacg gataatgagc ctcatcagcc ttgctttcaa cggggtgatg cccttttcaa
     2101 cgctctccct ctcggctttg gcgcagctga ttggcctgga tatcgtcatt ggcatcagtg
     2161 cggtgttgct cgccttttgc ggcgtgtacc tgtggcgacg ctttgtatgg aaggcgttca
     2221 tccctgaggt gatgccaggt tccggcgcac agcattccct gtaagttccc aacccaatgg
     2281 catcttgaac acccacctgc agtgaacgac cgtgccggca tggtgtttta cgacccggcc
     2341 gggccttcct ccggtcatcc agccgcctag gtccggcact gattcctcct gttggcgagg
     2401 tgtgttggcg tgacccccgc gaccgccgat gcaggggagt gattgtcaaa ttgatccaca
     2461 tcgctccatc ccgctatgca ggaggttcta cccatgttct ttcacgtcaa ggagttgcag
     2521 ttcgaggcga agctagaccg gccgggcccg ctctatgcca agcggctcca ggaattgctg
     2581 ggcggcaagt tcggcgagat gaccgtcatg atgtcgtacc tcttccaggg gtggaacagc
     2641 cgcggtcccg agaagtaccg cgatatgctg ctcgacatcg ggactgagga gattgcgcac
     2701 gtcgagatgc tctccgtgat gatctcccgt ctcctggaca aggcgccggt gaaggagcag
     2761 gaggacgcgg ccaagatccc cgtcatcggt gtggcccttg gtggtggcaa tcccagcgac
     2821 gtcatctttg ccgcaatgaa cccgcagcac gagatcgtca ccggacaggg agcgaatgcc
     2881 catgacagcg tcggtgtgcc gtggagtggt gcctatgcca ccgccagtgg gaacctgatg
     2941 gccgattttc gctggagcct gatggcagag tcgcagggca acctgcaggt gagccggctc
     3001 tacgagatgg ccgaggacca ggacatccgc aagatgctgt cgttcaacct ggcgcgcgac
     3061 atcatgcacc agaaccagtg gcaggcggcg atcgaggaga tccagaccga tggcctgcac
     3121 cagttccagg tgccgagccc cttcccgctc gatcgggtga acctctccca ggcgtacacc
     3181 ttctggaact gctcgaaggg cacggcaagc gaggagggtc gataggccag cgggccgaca
     3241 cccgacggca acggggagtt cgtgtacctg gccgattccg cgcccctgac cgacgacatt
     3301 ggcgagttgt cgcaactcga cccgcgcctg catggcaagg cgaaaatccc aatgccgctg
     3361 atgtccagcg gaccgaaccc ggtaccggat caggacattc cgaagaacgt caacaagaat
     3421 gccgtcaccg gcgacggcaa gatgtaacgt gagcggcgta tttaaccagt agccatcaca
     3481 caccaatgag cttccggagc gcaggtgttc cggaagctca ttgcatcttt cgccggggtc
     3541 gggagtggcg tgtttgcctc tcgcctccct gccaaaacaa ccaccttatt ttggtcgtcc
     3601 tcaggctccg cagggtgcgg cgaagtcaac atgcgggatc gggcaactct tcccgggagc
     3661 gatcgtcctg acaggagatg tcgtacgggg ctcatcatct ggcggctagc ccgtgtccac
     3721 ctcagggcgt cggggatgtg gtcgcctccg ggtcccgcac ctggtcaagc gagagcgttc
     3781 gcccctcccg ggctgactgg acgatctcct tctctgcctc ttcctccacg atctcgggaa
     3841 tgggaggctg ctgcgagagg cgctcctccg caacatcgga tcgcatcgcc gccagcagcg
     3901 ttggtccatc ctcgcggacg cggagccgaa gccagaaaaa ctccccgacc acacgcagga
     3961 ccgccaggat tggcactgcc atcaccgccc cgatcccacc gaacatgctg gaaccggcga
     4021 tcacgctgaa gaagatcagc agcggatgca cgcgcacggc ggatccctgg atgcgcgggg
     4081 tgatgaagtt tgcctcaagc tggttgatca cgatgaagag cagcgtggtc agaagggcca
     4141 gctgccacga gatcgtgaga gcgatcgcga cggcggggat cgcgccgagg aaggccccga
     4201 tatagggcag gattgccgtc atcgatgtcc agatcccgag aagcagggca tacggaacac
     4261 cgaggacagt gaggccgatg aaggctgcca ctccctggat caggatggag gcgagcaacc
     4321 cgctcaggta gcgggagagc gagtagccga gcgtatccca gagaaccacc gcatcattcc
     4381 ggtaggcggg tgcccacatg cggacaaagg agtccctgaa ccgcggaatg tcggcgacga
     4441 gatagatgga gacaaagatc accccgaacg tcgtgatgag caggttcact gaacgggtca
     4501 tccagcctac ggtattggtg aggagatccg tgaccattcg ctgtgcagcg tcgaagatac
     4561 ctgcctcgat gtcgctcagc acttcatccg gatgctgctc catccatcca cgatcgtaga
     4621 actggaagag gagatcccgg gtcagcccct gaaggtcatc aactgtcgcc gggaaggatt
     4681 cggcgaaccg gcttatctcg ttgatcgcga aggggatcag cagcgcgagc aacacgatcg
     4741 cgaaaagcag cgtggagaac gcgacgatcg tgatcgcgac cctgcggggc aggaacgcgt
     4801 tgaggaagcg caccggaaac gagagcacca gcgcaacgat cgcacccata aagagcagca
     4861 ggacgacgtc cgatgcgcgt gtggcgatga gatagaacac cgcgccgatc agcaggagca
     4921 ggatcaaccg ggtgcgtggt gtgatgtaga tcggggtcgg cagggcaagc ggaagtggcg
     4981 gtccgtagac caccggccct
//
LOCUS       DASYWM010000146         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_662097, whole genome shotgun sequence.
ACCESSION   DASYWM010000146 DASYWM010000000
VERSION     DASYWM010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662097"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VGR29_05915"
     CDS             <1..595
                     /locus_tag="VGR29_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073162.1"
                     /translation="RGVRDTGDRRGAPHLGHDRVRALRDRRFHPTRCEGRRDNGGALD
                     TADMAYYGPTGRAENIMNLWADAVLVDLAANAIRLRVPGLDGCEASIGVLDVGANGRL
                     LGLEIGEAYITVMESSRHEEPYTRSAVVALAISGDDPAYVAIPRRGPEYEITYPSGNE
                     CWQLTTVDGQLIQICAVIGDARVEPAGGSALEKGNRL"
     gene            1138..2139
                     /locus_tag="VGR29_05920"
     CDS             1138..2139
                     /locus_tag="VGR29_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073163.1"
                     /translation="MVAHNDRFSRRTFAALGLGASAGLLAATRSVTAQDASPAASPEV
                     AEAGAFENTGFTEVVVTAAQYTYSASVPGAMGEGWYIFTLVNETESVADLNLGLLPEG
                     TTGSDLSSTLATAFQGEGGELPEWWTSALFAGGGVAGPGESTSSLVYLTPGKWYMFST
                     NPASTQSPASFTIQTPEELEANEGIIATPTASPVGVGTPGATPEIAGLVNPEGVTAAV
                     SIEVTENQIVPNGAPTGGEQVLQITNTGDQVRDMIILQTEETLDEAGAVALATSWIRG
                     EETNATVAGGVGALSPGWTAFTEVDVQPGNYVVFSAMPDANGGLQVENGIITTFSGQ"
     gene            2392..2586
                     /locus_tag="VGR29_05925"
     CDS             2392..2586
                     /locus_tag="VGR29_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073164.1"
                     /translation="MEELSRILTAAGASVFGVGITIYGIGTSIFEAGGFEETVGLTLT
                     AIGAVATLIGLILFRQTAED"
     gene            complement(2657..>3031)
                     /locus_tag="VGR29_05930"
     CDS             complement(2657..>3031)
                     /locus_tag="VGR29_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073165.1"
                     /translation="RRIGIIGLLDAKNGKEMIRSVLPVIDVLIVTAPKVLGKDAKSVG
                     SIVDLARDAGFRGEVLVEPDSLRTMDLAYSQVQEARGDAVLVTGSLYLVGNVRERWYG
                     EREIVAQRTPWPRVSASSSQGP"
BASE COUNT          582 a          934 c          934 g          581 t
ORIGIN      
        1 gcgaggtgtc agggatacag gagacagacg aggggcaccg catctggggc acgatcgagt
       61 gcgtgccctc cgcgatcggc gcttccatcc aacccgttgc gaaggtagga gggataacgg
      121 tggagccctt gacaccgcgg atatggcgta ttacggtcct accggcaggg cagaaaacat
      181 catgaacctt tgggcagacg ctgtactcgt cgatctggcc gcaaacgcga ttcgactccg
      241 ggtccctggt ctcgatggct gcgaagcatc catcggtgtg ctggacgtgg gagccaacgg
      301 tcgactccta gggctggaga tcggcgaggc gtacatcacc gtgatggagt cgtcccggca
      361 cgaggagccc tatacccgga gtgcggtcgt cgctcttgcc atctccggcg acgatccagc
      421 ctatgtcgcg attccgcgtc gtgggcccga gtacgagatc acctatccga gcggcaacga
      481 atgctggcag ctcacgaccg ttgatgggca gctcatccag atctgcgccg tgatcgggga
      541 cgcacgcgtg gagcctgccg gcggctccgc actggagaag gggaaccggc tgtgaatcct
      601 gccaggatct cccgggcagc ttccatatcg tcctggcgta catagagcgc gtgctcaaaa
      661 gtggccgatg acgcccaggc acccgcgccg ggaccaccag gacgaagcag gaccgctatg
      721 ccctcgctgc ggagcgcgtc cgcccacatc acaccctcga cctccgaccc tgccgtgatg
      781 aggtaccgga tgtccgcggg ctccttgccg gaggagttgg acgggctggt tcctggttgg
      841 tctatcgaat ctcgtccttg cttcactcgc cgttcacctt ccttatccca tcagacagtt
      901 gtccggagtc tgctacgggt tgtccacccg ggtggacatg cttgggctcg cgcctgcata
      961 tcggcactcc tgaccctgag tatagttttg tactgcagga tcggtcggga cattaccgcc
     1021 tggcctcgta tgggtgcgat atcggctgat gcggtggccc ttatcctgca acggaaatac
     1081 gctggtcagg tttttgtttc tgcgtacggg ttcaacgccc aaaggagctt gttgccaatg
     1141 gttgcacaca atgatcgatt ttcacgacgg acgttcgccg cgctggggct gggtgcttca
     1201 gccggtttgc ttgcggcaac gagatccgtc acagcccagg atgcgtcacc agccgcatcg
     1261 ccggaggtgg ccgaagctgg cgcgttcgag aacactggtt tcaccgaggt tgttgtcacg
     1321 gcagcgcaat acacctacag cgcaagcgtt ccaggcgcga tgggtgaggg gtggtatatc
     1381 ttcacactgg taaacgagac agagtcggtc gctgacctca atctggggct tctcccggaa
     1441 ggcacgacgg gaagcgacct gagctctacc ctcgccacag cgttccaggg tgagggagga
     1501 gagctgccag agtggtggac ctctgcttta ttcgctgggg gcggcgtggc aggcccgggc
     1561 gaatccactt cctccctggt gtacctcacg ccaggcaaat ggtacatgtt cagcaccaac
     1621 ccagcttcaa cccagtctcc ggccagcttc accatccaga caccggaaga acttgaggcg
     1681 aacgagggca ttatcgctac accgacggca agtccggtgg gcgttggaac gccaggggca
     1741 accccggaga tcgctggtct cgtcaatcct gaaggggtca ccgcggcggt ttccatcgag
     1801 gttaccgaga accagatcgt tccgaacggc gctccgacag gcggtgagca ggtcctgcag
     1861 atcaccaata cgggtgacca ggtccgcgac atgatcatcc tccagacgga agaaaccctc
     1921 gacgaggcag gtgccgtggc gctggctacc tcgtggatca ggggtgagga aaccaacgca
     1981 acagtcgccg gcggtgtggg cgccctcagc ccagggtgga cagccttcac tgaggtcgat
     2041 gtccagccgg gcaactacgt cgtcttcagc gcgatgccgg atgcgaacgg cggcctgcag
     2101 gtcgaaaacg gcatcatcac aaccttctcc ggccagtaag caacgcgaag gctggttgag
     2161 tacaggaggc atggcgcatc catgcctcct ctcacatccc gggcatctct acgcgtttcc
     2221 cgagcaaaga ccctcgacta ttccagccct tttcagtagg ctacggagag cgccgggtct
     2281 ggtgacgccg tccgtacccg cccggtacga tgcgatatct cgtacagagg ccatatcatg
     2341 aatccgttgg agcggcggtc accgatccgt ctggggacga ggggaagagt catggaagag
     2401 ttgagccgta ttcttactgc tgccggggcc tcggtgttcg gcgtaggtat caccatctac
     2461 ggtatcggca cctcgatttt tgaggcgggg ggcttcgagg agaccgtcgg gcttaccctt
     2521 acggcgatcg gtgcagtcgc gacccttatc ggactcatcc tcttccgtca gactgccgag
     2581 gactaatcgg cgtatacgga gaacacggaa cagccccgct tgcgctccat ggagtgcaag
     2641 cggggctgtt ccatgttcag gggccttggc tggagcttgc cgagactcgg ggccatggag
     2701 tgcgctgagc gacgatctcc cgctcgccat accagcgctc gcgtacattg cccaccagat
     2761 agagtgatcc ggtgaccaga acagcgtcgc cccgtgcctc ctgaacctgg gaatacgcca
     2821 ggtccatcgt ccgcagcgaa tcaggctcga ccagtacctc gcccctgaag ccggcgtccc
     2881 gggcgagatc aacgatcgaa ccgacactct tggcatcctt ccccaacacc ttcggcgctg
     2941 tcacgatcag cacatcgatc accggcagca cgctacgaat catctccttg ccattcttcg
     3001 cgtcgagcaa tccgatgatc ccgatccggc g
//
LOCUS       DASYWM010000147        20598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_136923, whole genome shotgun sequence.
ACCESSION   DASYWM010000147 DASYWM010000000
VERSION     DASYWM010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 20598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20598
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136923"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VGR29_05935"
     CDS             <1..714
                     /locus_tag="VGR29_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2073166.1"
                     /translation="EISRSPFALPTELRWENIERAWTVGGFDRYVLNTVIYCAAIVTG
                     VVALSCFSGYALALLPLPGRGVVLTLFLLGLMVPFQSVMIPIYYLLRDIGLLETYWAF
                     IVPGIALRLPFGIFLMRGFFKGLPIDIADAARMDGANEWQVFRSIMLPLAIPGLSTLV
                     VFQFIYTWNQFLMPLIFVQRDELRPVALGTMFFFGRFTADRGMIAAGVTISMIPMVIL
                     YLLLQRRFIEGITAGAMKT"
     gene            819..2243
                     /locus_tag="VGR29_05940"
     CDS             819..2243
                     /locus_tag="VGR29_05940"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073167.1"
                     /translation="MTVDRNISGPSPAKGLPTAGNGALADGRKGNKDSFKTTPTIRRT
                     GALRWPTTRWLRDVQLPHALQVAPFRNYWLSQIVAWAGTWMQQVGIQLVVLSLTTSAL
                     AIGMINIVSAVPMLLLSLTGGVIADRFNRRHILMVSMSLLGLFSLVYAWLIGTDNIEY
                     WHILVFASLGGVVMSFQLPAAQAFVAELVPRDDLPQAIALNSASFNSTRIVGPALAAS
                     AIGAQGLASAFIMNAIALLAPIGTLFRLRKVLKAPIRARDSGSQFEALKRGLGYVKSD
                     DGTMGLMLLQAVISFFVTPPLLVLLPLYVTNELGGSDSWVGIMLSFMGAGSLTGALVL
                     LRGSRLESAAARRLMIAVAGLALGMGWIALSQNPVMAVPGVMVGGFSFSMANSQIMTR
                     LQQTAPDELRGRLMSLNSLAFNGVMPFSTLSLSALAQLIGLDIVIGISAVLLALCGVY
                     LWRRYVWKAFLPEVIPGSGAQHSL"
     gene            2631..3644
                     /locus_tag="VGR29_05945"
     CDS             2631..3644
                     /locus_tag="VGR29_05945"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEV2073168.1"
                     /translation="MTPATADAGERSLYRSASLHPANQEVPFMFFHVKELQFEAKPDT
                     PDPLYAKQLQELLGGKFGEMTVMMSYLFQGWNSRGPEKYRDMLLDIGTEEIAHVEMLS
                     VMISRLLDKAPVKEQEDAAKIPVIGAALGGGNPSDVVFAAMNPQHEIVNGQGADAKDS
                     MGLPWSGAFATASGNLMAESQGNLQVSRLYEMTEDQGIRKMLSFNLARDIMHQNQWQA
                     AIEELQADGLHQFQVPSPFPLDRVNLSQAYTFWNCSKGTASEEGRWASGPTPDGNGEF
                     VYLADPTPLTDDIGELSQADPRLHGTPKMPMPPMSSGPNPVSDQDIPKNVNKKAVTSD
                     GKM"
     gene            complement(3914..5185)
                     /locus_tag="VGR29_05950"
     CDS             complement(3914..5185)
                     /locus_tag="VGR29_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2073169.1"
                     /translation="MVYGPPLPLALPTPIYITPRTRLILLLLIGAVFYLIATRASEVI
                     LLLFMGAIVALVLSFPVRFLNAFLPRRVAITIVAFSTLLFAIVLLALLIPFAINEISR
                     FAESFPATVDDLQGLTRDLLFQFYDRGWMEEHPDEVLSDIEAGIFDAGQQVVTDLLSN
                     TVGWMTRSVNLLITTFGVIFVSIYLLADIPRFRDSFVRMWAPAYRDDAVVLWDTLGYS
                     LSRYLSGLLASILIQGVAAFIGLTVLGVPYALLLGFWTSMTAILPYIGAFLGAIPAIA
                     IALTISWELALLTTLLFIVINQLEANFITPRIQGSAVRVHPLLIFFSVIAGSSMFGAI
                     GAVMAVPTLAVLRVVGEFFWLRLRVREDGPTLLAAMRSDVAEERLSQQPPIPEIVEEE
                     AEKEIVQSAQEGRTLSIEQVRDPEATTSPAP"
     gene            complement(5322..5759)
                     /locus_tag="VGR29_05955"
     CDS             complement(5322..5759)
                     /locus_tag="VGR29_05955"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV2073170.1"
                     /translation="MADTVVNIEVIVVRNDRFLTIVRSADEEFGGGWLCFPGGKLDPG
                     TADPDAIEATAQRELREEVGLEVAIDGLIYVESHTFMIGNETILDIVMMTETATGEAV
                     ARDPAEVAEVLWLTEDEIMDDPRVQSWTRESLRRAIARRCRAR"
     gene            6085..6951
                     /locus_tag="VGR29_05960"
     CDS             6085..6951
                     /locus_tag="VGR29_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2073171.1"
                     /translation="MNQVLITGASGKLGHATVREMTAHGFDVVAIDRVRPREAHPEGV
                     RAVEIDLLDIGQVAGAMTGCDAVIHLGAIPAPYGHADEVVFRNNTGATFAVLQAALLL
                     GIRRIAIASSGSIYGTAWSKTPLFFHYAPVDEAHPLMVHDAYALSKETDERTAEMFCR
                     RDGMSIAALRFHWIATQDEQLAVVAQRRAKTDRSDEASGLWGYTDLRDAARACRLAIE
                     AAREGPFGFAPLNIVAADTLADEPTEDLVRRWSPGTEVRHSIPGTSGAYDTSTAKRLI
                     GWEAKHSWRDVG"
     gene            7671..8090
                     /locus_tag="VGR29_05965"
     CDS             7671..8090
                     /locus_tag="VGR29_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2073172.1"
                     /translation="MKLDGQQPHILVVNDSPEILALKREIFEEEGFQVSTRINRDVDL
                     DEIVRIGPNLVILDYSDEAESALLGQLRGDPRTASIPIVLCTGAIRKVEEIRPQIEAM
                     GVRVVYKPFDIEDLVGVVREALDMDSERQETPPPGAA"
     gene            8187..8471
                     /locus_tag="VGR29_05970"
     CDS             8187..8471
                     /locus_tag="VGR29_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEV2073173.1"
                     /translation="MEPMEPRDTNQPNGQGNSDHGSSEAMQHHIRTPLSVIYGHVQLL
                     QRRVRRGEVPDQKDLLRTLGYIEQAARAIEAQLRSPSEKADPPARNADDE"
     gene            8620..9051
                     /locus_tag="VGR29_05975"
     CDS             8620..9051
                     /locus_tag="VGR29_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2073174.1"
                     /translation="MSADGHGEVGGRATPEQMARKHIYVINGSPEFLGVIRELLQDEH
                     YNVTTTNFIPNSFETIETAQPSLLIVDLILGEQAGWDLLGRLHEAMSTRHIPVVLVST
                     TPRLLEEAKERHELFGGDRYLAKPFNLDDLLGLITELIGKA"
     gene            9367..9816
                     /locus_tag="VGR29_05980"
     CDS             9367..9816
                     /locus_tag="VGR29_05980"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2073175.1"
                     /translation="MDVIVWNEGEGEILGSGPYQLRFLMQSPDQPVAITENSVPPHFP
                     GPVSHRHVRMTDIFYVVEGTLTLHFGSEQRPLGAGGFALVPPGVVHTFSNEEDVPVRF
                     LNIYQPSGNEHYLKEVGQRVAAGSPPSKAEMAEIAARYDFVPVVDEE"
     gene            complement(9938..11029)
                     /locus_tag="VGR29_05985"
     CDS             complement(9938..11029)
                     /locus_tag="VGR29_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2073176.1"
                     /translation="MNDRPTGIGVIGAGFISAIYLKNLTSRWDHVRVVGVADLIADRA
                     QARAGQFGIHAFTVDELLAHPEIEIVLNLTIPAAHAAVCFGVIEAGKSVYTEKPLTLD
                     RGEGERLLHAAHQRGVLVGCAPDTFLGAGLQTVRRLIDEGAIGEPVAFSARMTTHGHE
                     SWHPEPAFYYQPGAGPMLDMGPYYVTAIATLLGPIRRVSAMTRITFPRRTITSQPRYG
                     QVIEVNTPSHFETNLELESGVIGTLTTTFDLWDPEHCAFTIYGSEGSIRLPDPNTFGG
                     PIQLLRGGEKTWQDVPLDPGYDTDTRGIGVADLAHALRTGQPARASGDLGFHALDVML
                     AALESAEQGRHLEIGSTVARPRPLETIGV"
     gene            complement(11183..12274)
                     /locus_tag="VGR29_05990"
     CDS             complement(11183..12274)
                     /locus_tag="VGR29_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2073177.1"
                     /translation="MSDAINPVGIGIVGAGVISEVYLKNLSTLFNHTKVIGVADVLAE
                     RAKSRADQFGIEALTVEELLAHPKIELVVNLTIPAAHADIALRALEAGKSIYNEKPLA
                     IARQEGKRILEIAQEKGLHVGSAPDTFLGGGLQTVRQLLDEGIIGEPVAIHAALLGHG
                     MEHWHPDPFFFFQPGAGPLFDVGVYYITALAALLGPFASVSALARASFPERTIGNGPK
                     LGEKIPVTTPTHIVSSVEFESGVLGSLTASFDVWETEFANLTIYGADGTLRLPDPNTF
                     GGPIKLLVGDSKEWQEIPVTKPHTENSRGLGAADLAMSMRTGHPARASGELGYHVLDV
                     MHAILESAERGTHIAVESTVARPAPAGGE"
     gene            12474..13517
                     /locus_tag="VGR29_05995"
     CDS             12474..13517
                     /locus_tag="VGR29_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2073178.1"
                     /translation="MTLKLIHVGLGRWGMDWETTALEAIRDRVETVAVVEAFEPTLEK
                     AKATLKLSDDQCFATLDDALKSVESDAVLITAPMDAHVPLAIKAMRAGRHVLVEKPLA
                     GSVEEAKEAVQVAEETGKVLQVSQNYRFYPAARKAAELVHNEALGDLGTINIDFRQWG
                     NEAEEGTNPHYRFVHPLIFDMAIHHFDLLRLILRQEPVRIYVQQSDPHWSKYVEEAAA
                     TMVITFADGTVVSYRGSWVSSDLRTYWTGDWRMECEQGLIYWTGRQGGPHGTDGDKVT
                     IRTRDGKVKDVALDEMPIWGRSAGVLAFIEAIETGIEPENSARRNLGSIALMEAAAKS
                     MASGQVEDVEVVS"
     gene            complement(13624..14982)
                     /locus_tag="VGR29_06000"
     CDS             complement(13624..14982)
                     /locus_tag="VGR29_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2073179.1"
                     /translation="MADIATTDVARNLQNAAVFHLQNLLRCDTQSPPGNERIAAEYIK
                     GALAEEDIDCTFVEAAPGRVSVVARLEAENPTARPVLLMGHVDVVTVERDKWDRDPFG
                     GELIDGFVWGRGAVDMKGQVAAELAAFLHIKRSGIPLNRDLIFVAFADEEAGGAFGAD
                     YVWKHHQELIDAEFAINEGGGSPIEIANHLFYTCQVGEKGGTRLKMTVRGEPGHASVP
                     QSNTAMEKLAEALHRLHDWEPPTQITASVRLMLEGIAGALGSDTGDSIRSLLEQGSPS
                     WAELAKLPLPENEKAHFRAITRNTAVPTIIHGGRQINVIPSEVVVEIDGRILPGEEPE
                     AFRAAVQEAVGDVAEITFLYETQESGIEADPESAFFEAIKETMSALRPDATVVPTLIS
                     GGTDAGLVPGVKVYGFFPALPTERIPLYDTLVHGHNERLHIDDLGFGTRFIYHLVVRF
                     CG"
     gene            15244..15894
                     /locus_tag="VGR29_06005"
     CDS             15244..15894
                     /locus_tag="VGR29_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEV2073180.1"
                     /translation="MTINSSPSSTRKSTRLVLIAAVTALAVLAIAGAAWFFYLRPEPA
                     YAFHGGFYEPPNPAPSLDGAVDQHGNPFKLEDYRGKLVFVYFGYTHCPDACPATLNEF
                     LEVKDALGEDAEDVAFVMITVDPARDTPPQLAEFLASWDPAFYGVSMPETETEAVAGA
                     WNVHYNHHHADPGDGYLVDHEVSSFVVDKDGNIRLIYPLGFDTGLMAEDAEYLLGE"
     gene            16018..16794
                     /locus_tag="VGR29_06010"
     CDS             16018..16794
                     /locus_tag="VGR29_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEV2073181.1"
                     /translation="MSVMIWNGMTREALGSALPDSVVVLPTAATEQHGPHLATGHDAL
                     VLDEIVRRAAGQVDVRTNVVVAPTLAFGSSDHHLPFGGTLSLRSETYLAVVKDLLRSM
                     IESGARRVLVVNGHGGNHELNEIAVRDVALVQPAEQPVVLAAGSWWAITAKALAGREE
                     FGGVRVPGHAGQLETAMMLALQPELVEDGRPAREHDPSLGTRIAGLRVEGEVPWRQFD
                     GFTDSPALATPALGALALDLAAGALAQAITRVADAGTAAA"
     gene            complement(16995..17630)
                     /locus_tag="VGR29_06015"
     CDS             complement(16995..17630)
                     /locus_tag="VGR29_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV2073182.1"
                     /translation="MDAGWRAVMVHMTGGEKGHLKLTPEEYLTIKEEEALNAAKLLGA
                     ESVMMPWADGELLVNEEVQMAVADVIRQYRPNSILTHWKGSFHRDHNNTYYNVLASLF
                     FAGLRTFVREYAPHRPEKIYFAENWEDVEDFRPEIYLDTTDVFDRYIEAIHAYSLFRG
                     EVVPFQYEQWYRGASEMRGAEAGFKRAVALMPYKTYFGSRTNVELLGTGNE"
     gene            complement(17820..18779)
                     /locus_tag="VGR29_06020"
     CDS             complement(17820..18779)
                     /locus_tag="VGR29_06020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2073183.1"
                     /translation="MAMADFTIRAYAETDEAGLLRTWNSGLYADPINATRWRGKVLLD
                     PNFDREGCLVAEMNGEVRGFLLSLVRRVPFFNDGLEPEQSWITAFAVDPAWQRQGIGR
                     ALLHAAIDRLRSLGRTSVALAPYVPNYFTPGADVATYGPGIDFLTREGFEVIERPISM
                     RADLTGFTTPEPVRERQQALAAEGVEVRLVTPADIAPVLDFIPRHFTWDWHREATGVF
                     NDLYNGDPRFVGMIVARQDGEVLGYAQHRGERFGPFGVRPDLRSRGIGRVLLAETLAG
                     MLQKNFHAAWFLWTGDDAARLYGQLGFHQVRRFAVMRKDISGQ"
     gene            complement(18781..19626)
                     /locus_tag="VGR29_06025"
     CDS             complement(18781..19626)
                     /locus_tag="VGR29_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2073184.1"
                     /translation="MSTSMKFAAFFKPVVTYGILALGAFVMLLPFAWMLSSSLMSTQE
                     VIARPLTWIPSALRFENYEALSDAIPLGRMYVNSIIVTTLTTLGILLTSSLAGYGFAK
                     FQFAGRDFLFLLVLATMMIPFFVVLLPIFYMVSQLGWINTYQGLIVPNIVTGFGIFLM
                     RQYMLSLPDEVLDAARIDGASEFEIYWRIALPLSTPVIGALGILAFVYQWNNFLWPLV
                     VARSSDMWTVPVGLNSLRVYASSAEVINLQMAGAALAIVPVMIVFLLLQRYFVRGIAL
                     TGMKG"
     gene            complement(19623..20558)
                     /locus_tag="VGR29_06030"
     CDS             complement(19623..20558)
                     /locus_tag="VGR29_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2073185.1"
                     /translation="MQQRFPAVLPATTSITTRRRRLIGRKAFAGYMFVLPVVLFLLAF
                     SLAPFLFTIWVSLHQWNMLTPVGDMPFRGLENYQYLLRDDPLFQETFRTTVIFALANV
                     GISVVFALCIALILNGPVRYRPFWRAAFFIPYVTVPVAIAIVWRSILNDRYGMLNGLL
                     NQMGLPQQPFLDSVSQALPSVIGVAVWQGIGYYMIIFLAGLQVIPGDLYEAAKLDGAN
                     AWRLFWSITLPLLRPTMLFVVVVNTLNSLQIFDLPFILTQGGPVNATNRLVLYMYETA
                     FSFLRVERATAMAVLLFLVIFVITLVQLRLLRERK"
BASE COUNT         3870 a         6204 c         6627 g         3897 t
ORIGIN      
        1 gagatctcgc gcagcccctt cgcgttgcca acggagctgc ggtgggagaa catcgagcga
       61 gcctggacgg tcggtggctt tgaccgctac gtgctcaaca ctgtcatcta ttgtgcggcg
      121 atcgtcaccg gggtcgtggc cctgtcctgt ttctcggggt acgcgctcgc gctgctgccg
      181 ttacccggca ggggcgtggt cctgacgctg ttcctgctgg gactgatggt gcccttccag
      241 tccgtgatga tcccgatcta ctatctcctg cgggatattg ggctcctgga gacctactgg
      301 gcgttcattg tgccgggtat cgccttgcgg ttaccgttcg gcatcttcct catgcggggc
      361 tttttcaagg gattgcccat agatatcgcc gatgcggcgc ggatggacgg cgcgaatgag
      421 tggcaggtgt tccggagcat catgctgccg ctggcgattc caggcctctc cacgctggtt
      481 gtcttccagt tcatctacac ctggaaccag ttcctgatgc cgctgatctt cgtgcagcgt
      541 gacgagctgc ggccggttgc actcggaacc atgttcttct tcgggcgctt caccgccgac
      601 cgtggcatga tcgcggctgg tgtgacgatc tccatgatcc cgatggtcat cctctacctg
      661 ctgctgcagc ggcgcttcat cgagggcatc acggcgggtg cgatgaagac ctgattctca
      721 ttgcgagtct ctcctcaaca tggttgcccg gtgtgcattc tcgacgataa tgcacatcag
      781 gcaaccgtgg cgatcgcgcc gcgaggggac gacacacgat gaccgtagac aggaatattt
      841 cgggcccttc gccggcgaag ggtcttccca ctgctggcaa cggtgcgctg gctgatgggc
      901 gcaagggcaa caaggactcc ttcaaaacca cccctacaat ccggcggact ggggcgctac
      961 gttggccgac gacgcggtgg ctgcgcgatg tacaactccc tcacgccctg caggtcgcgc
     1021 ccttccggaa ctactggctc agccagattg tggcctgggc cggcacctgg atgcagcagg
     1081 ttggaatcca gctcgtggtg ctctccctaa cgacctcggc actcgctatc ggcatgatca
     1141 atatcgtgtc ggccgtgccg atgctgctgc tcagcctgac cggtggagtg atcgccgatc
     1201 ggttcaaccg ccggcatatc ctgatggtct cgatgagtct gctaggtctc ttctcgcttg
     1261 tctatgcctg gttgatcggg accgacaaca tcgagtactg gcatatcctc gtctttgcct
     1321 ctctgggcgg ggtggtcatg tctttccagc tcccggcagc ccaggcattt gtcgccgaac
     1381 tggtcccccg ggacgacttg ccccaggcaa tcgcgctcaa ctcggcttca ttcaactcca
     1441 cgcgtatcgt cgggccggcg ctggccgcgt cggcaatcgg cgcgcagggg ctggcgagtg
     1501 ccttcatcat gaacgccatc gcgttactgg ccccgatcgg cacgctcttc cggctacgca
     1561 aggtgctgaa ggcgcccatt cgggcgaggg actccggatc gcagttcgag gcgctcaagc
     1621 gtggcctcgg ttacgtcaag agcgacgacg gcacgatggg actgatgctc ctccaggcag
     1681 tgatctcgtt cttcgttacc cctcccctgc tggtcctgct gcctctctac gtgacgaacg
     1741 aactgggagg cagtgatagt tgggttggca tcatgctttc gttcatgggt gccgggtcgc
     1801 tgaccggtgc gctcgtgctt ctgcgcggaa gccgcctgga atccgcagcc gcacggcgcc
     1861 tcatgatcgc cgtggccggt ctggcgctgg gcatgggctg gatagcgctc tcgcagaatc
     1921 ccgtcatggc ggttcccggt gtgatggtcg gtgggttctc cttctcgatg gcgaactccc
     1981 agatcatgac acgcctgcag cagacggctc ccgacgaatt gcggggccgg ctcatgagcc
     2041 tcaacagcct tgccttcaac ggggtgatgc ccttttcaac gctctccctc tcggcgctgg
     2101 cccagctgat cgggctggat atcgtcattg gcatcagcgc cgtgttgctc gccctttgcg
     2161 gcgtgtacct gtggcgacgc tatgtgtgga aggcgttcct ccctgaggtg ataccgggct
     2221 ccggcgcgca gcactccctg taaacactca accaacatta acttgcagtg agattgatac
     2281 gccaagcttg ccaggcagaa ggtgtagcga agggctcgct gttgctgttc gccttgcagg
     2341 ctcagcctgg ggctcgctgc caggggacat tgctgcatcg tggccgcgaa caggccgtat
     2401 gcaccgcaag tcctggggtg agccctcagg cgaatagcgg gagcgacccc ttcgctacag
     2461 acgctacaga gatccaaccc atctgaaaac agctttaacg gcaacttgaa tgcccatctg
     2521 cagtgaacga ccgtgccggc ctggtgttta acgacctggc ggagtcttcc tccggcaatt
     2581 gggtcacccg ggtacggcac tgattgctcc gcttgaagga gcatgctggc atgacccccg
     2641 cgacggccga tgcaggggag cgatcgttat atcgatccgc atcgctccat cccgctaatc
     2701 aggaggttcc atttatgttc tttcatgtca aggaattgca gttcgaagcg aagccggaca
     2761 cgccggatcc actctacgcc aagcagctcc aggaattgct gggcggcaag tttggcgaga
     2821 tgaccgtcat gatgtcgtac ctcttccagg ggtggaacag ccgtggtccc gagaaatacc
     2881 gcgacatgct gctcgacatc gggacggagg agattgccca cgtcgagatg ctctccgtga
     2941 tgatctcccg tctgctggac aaggcaccgg tgaaggagca ggaggatgcc gccaagatcc
     3001 cggtgatcgg tgcggccctt ggtggcggca atcccagcga cgtcgtcttt gccgcaatga
     3061 acccgcagca cgagatcgtc aacgggcagg gcgcggatgc caaggacagc atgggcctgc
     3121 cgtggagcgg cgccttcgcc actgccagcg gtaacctgat ggccgagtcg cagggtaacc
     3181 tgcaggtgag ccggctctat gagatgaccg aggatcaggg catccgcaag atgctgtcgt
     3241 tcaacctggc acgtgacatc atgcaccaga accagtggca ggcggcgatc gaggagctcc
     3301 aggctgacgg cctgcaccag ttccaggtgc cgagcccctt cccgctcgat cgggtgaacc
     3361 tgtcgcaggc gtacaccttc tggaattgct cgaagggcac ggcaagcgag gagggccgat
     3421 gggccagtgg gccgacaccc gatggcaatg gggagttcgt gtacctggcc gatcctacgc
     3481 ccctgacgga cgacatcggt gagttatcgc aggccgaccc gcgcctgcac ggcacgccga
     3541 aaatgccgat gccgccgatg tccagtgggc cgaacccggt atcggatcag gacattccga
     3601 agaacgtgaa caagaaagcc gtcaccagcg acggcaagat gtaacgcgat cagcgtatcc
     3661 agccagtcat catcacgcac taacgagcgc ccggaacatc tccgttccgg gcgctcgttg
     3721 cgtgtgccgg ccgagttggg agtgacgtgt ttgcctctcg cctcctcgcc aaaacgacca
     3781 cctggttttg gtcgtcctca gggtccgcat ggtgcggcga agtcgacatg caaaccggga
     3841 tcttctgcga gcggttgtcg tggaacgaga tgccgcacag ggtcgaacgc tcgatggctg
     3901 gccctcgtcc acatcagggc gccggggatg tggtcgcctc cgggtcccgg acctgttcga
     3961 tcgagagcgt tcgcccctcc tgggctgact ggacgatctc cttctccgcc tcttcctcca
     4021 cgatctcggg aatgggaggc tgctgcgaga ggcgctcctc cgcgacatcg gatcgcatcg
     4081 ccgccagcag cgtggggcca tcctcgcgga cgcggagccg aagccagaaa aactccccga
     4141 ccacacgcaa gaccgccaag gtcggcactg ccatcaccgc cccgatcgca ccgaacatgc
     4201 tggagccggc gatcacgctg aagaagatca gcagtggatg cacgcgcacg gcggatccct
     4261 ggatgcgcgg ggtgatgaag tttgcctcca gctggttgat cacaatgaag agcagcgtgg
     4321 ttagcagggc cagctcccag gagatcgtga gagcaatcgc gatggcgggg atcgcgccga
     4381 ggaaggcccc gatatagggc aggattgccg tcatcgatgt ccagaacccg agaagcaggg
     4441 catacgggac gccgagcacg gtgaggccga tgaaggctgc caccccctgg atcaggatgg
     4501 aggcgagcaa cccgctcagg tagcgggaga gcgagtagcc gagcgtatcc cacagaacca
     4561 ccgcatcatc ccggtaggcg ggtgcccaca tgcggacaaa ggaatccctg aaccggggaa
     4621 tatcggccag gagataaatc gagacaaaga tcacgccaaa ggtcgtgatg agcaggttta
     4681 ctgaccgggt catccagccc acggtattac taagtagatc cgtgactact tgctgtccgg
     4741 cgtcgaagat acctgcctcg atgtcgctca gcacctcatc cggatgctcc tccatccatc
     4801 cacgatcgta gaactggaag aggagatccc gggtcagccc ctgaaggtca tcgactgtcg
     4861 ccggaaagga ttcggcgaac cggcttatct cgttgatcgc aaaggggatc agcagcgcga
     4921 gcaacacgat cgcgaaaagc agcgtcgaga atgcgacgat cgtgattgcc accctgcggg
     4981 gcaggaacgc gttcaggaag cgcaccggaa acgagagcac cagcgcaacg atcgcaccca
     5041 tgaagagcag caggatgacc tccgatgcac gggtcgcgat gagatagaac accgcgccga
     5101 tcagcaggag caggatcaac cgggtgcgtg gtgtgatgta gatcggggtc ggcagggcaa
     5161 gcggaagtgg cggtccgtag accaccggcc ctcgtcgtaa aactcgcgtc ctgatcagat
     5221 cctgaagact acgcacggta tgcatcgaag ctctctcggt cggttgggaa ggcggcctga
     5281 cggcacgcat gtcgctgcgt atcaaaggca actgtagcgg atcagcgtgc gcgacaacgt
     5341 cgggcgatgg cacgccgaag gctctcccgg gtccaggatt gcacgcgcgg gtcgtccatg
     5401 atctcgtctt ccgtgagcca gagcacctcc gcgacctctg ccggatccct ggccacagct
     5461 tctcctgtcg ccgtttccgt catcatcacg atatcgagga tggtctcatt gccgatcata
     5521 aaggtgtggc tctcgacata gatgaggccg tcgatggcca cctccaggcc gacctcttcc
     5581 ctcagctccc gttgcgcagt ggcttcgatt gcgtcggggt cggcagttcc cgggtcaagc
     5641 tttccaccgg gaaagcagag ccaaccgccg ccgaactcct cgtccgccga gcggacgatg
     5701 gtcaggaagc ggtcgttccg gacgacaatg acttcgatat ttacgacagt gtcagccatg
     5761 tggcctcctg gtgtatctcg acggattccg gacacgatcg aggcgctccg tgtggagcgc
     5821 cccgatcgca atcgctcttc ggttccttct tcggtgtgaa tacagtatga catctggacc
     5881 gggtgcgcgc atccgtaata ttactgattt ccaaccgcct ggataaatca gcaacgtggc
     5941 tcctgaacga ttgccccagt ccattggacc gagttgctgt tccaagctcc cgtcttatca
     6001 gggagtacgg ttcctcgcac ggcgcttcgg gtcgtatcat tccgccacaa tatccgagga
     6061 cgacgatgaa gggatgattt gccaatgaac caggtcctga tcaccggcgc gagcgggaag
     6121 ctcggtcacg ccaccgtgcg ggagatgacc gcgcacgggt tcgacgtagt ggcgatcgat
     6181 cgagtccggc cgcgggaggc ccacccggag ggcgtccgcg ccgtggagat cgacctgctg
     6241 gatatcggcc aggttgccgg cgcgatgacg ggatgcgatg cggtcatcca tctcggggcg
     6301 ataccggctc catatggtca cgccgacgag gttgtcttcc ggaacaacac cggtgccacc
     6361 ttcgccgtgc tccaggcggc gttgctgctg ggtatcaggc gaatcgcaat cgcctccagc
     6421 ggatcgatct acggcacagc gtggtcgaag actcccctct tctttcacta cgcgccggtc
     6481 gacgaagcgc atccattgat ggtgcatgat gcttacgccc tctcgaagga gacggacgag
     6541 cggacggcgg agatgttctg tcgtcgcgac gggatgtcga tcgcggcgct gcgttttcac
     6601 tggatcgcga cgcaggacga gcagctagca gtggttgcgc agaggcgagc gaagaccgac
     6661 cgatcggatg aagcgagcgg cctgtggggg tacaccgacc tgcgggacgc tgctcgcgcc
     6721 tgccgtctgg cgatcgaagc cgcgcgggaa gggccgttcg gctttgcgcc gctgaacatc
     6781 gtcgcggccg atacgctggc cgacgagcca acggaggacc tcgttcgccg gtggtcgccg
     6841 gggacggagg ttcggcactc gatccccggc acatccggcg cctacgacac cagcaccgcg
     6901 aaacgcctca tcggctggga ggcgaagcac tcctggcggg atgtggggta agggtgcgtt
     6961 tcgggatgag cgttttttag gcggccacgt tggcaatctg ggccacttgg ccgtctaggg
     7021 cccgattgtg gttgattccc ttgatgtgat ggggctttct tcaggctgcc aggttgctgc
     7081 ggcgcgtggt cggaaacctg gcagcctaag gtgattaggc cgctttgcgg catgggcgca
     7141 aagccttcgg tcattcgccc atacggaatg agaagcgaat ggtccgagct cgcgtcagtc
     7201 tgcctagatg gccaggtttc aggtaagggg gatgttggca gtgtgagcgg tactgcaagg
     7261 cgagaagggg ctgcgagccc ttggctgagc ctgcgaggcg aaaagcagag cgagcccttg
     7321 cctacggcgt tgcacgatgc gacttttgta ggggtcggat tggatgcgca cgggaatcgg
     7381 gaaacaggcc atattggcgc gcatccaatg ccgtcccgag acggcggtgt ctgcccaaat
     7441 cggcgtgatt accggttccg acatcccaac gacatgctcc gttccctctg gtggattcct
     7501 cttgccataa gggcgtatgc tgttgagtat caactcgtct gatatcgtgc ttcggtcgaa
     7561 ccacatctcg gggcagccgg gggttccctc ccgtccctgc ttgcatgatg cgaccttgca
     7621 tcaccgtcga tgcgaccgtg gaaatcatcc atgcggcgaa aggaagggcg atgaagctcg
     7681 atggccagca gccccatatc ctggtggtga atgactcacc cgagattctt gccttgaagc
     7741 gtgagatctt cgaagaggag ggcttccagg tcagtacacg gatcaatcgt gatgtggacc
     7801 tggatgagat cgtccggatc ggtccgaacc tggtcatcct cgactacagt gatgaagcgg
     7861 aatcggctct tctggggcaa ctcaggggcg accctcgcac tgccagcatc ccgatcgtcc
     7921 tctgcaccgg agcgatacgc aaggtcgagg agattcggcc gcagatagag gccatggggg
     7981 ttcgggtggt ctacaagccg ttcgatatcg aggaccttgt gggcgtcgtg cgtgaggctc
     8041 tcgacatgga ttctgaacgg caagaaaccc cgccgcctgg ggccgcatag cataatcgcc
     8101 cagtgggcat agtctccggg gtacacccat tgcgtttccc tccacggatg gagcgaacga
     8161 cttcataccg tgaggatgtt tacgttatgg agccaatgga gcccagggat acgaatcaac
     8221 cgaacggaca ggggaacagc gaccacgggt cctccgaggc aatgcagcac cacatcagga
     8281 ccccgttgtc ggtgatctac gggcacgtgc agctcttgca gcggcgcgtc cgtcggggag
     8341 aggtgcccga ccagaaagat cttctgcgga cgctcggcta catcgaacag gccgcccgcg
     8401 cgatcgaggc ccagttaaga tcgcccagtg aaaaggcaga cccgcctgcg agaaacgctg
     8461 atgatgagta gggtggcagc gcgcagaaga ctctatgaac gtcgctgaag aatccccatc
     8521 agggtgagat tggcaatcag gactgcgatg gtacgttttc tgtcagtttc agtatccggg
     8581 gggcacgtta ctgagcgctg atggagggta acagcaggca tgagcgcgga cgggcacgga
     8641 gaggtaggcg gacgcgccac accggagcag atggcgcgca agcatatcta tgtcatcaac
     8701 ggatcacctg agttcctggg tgtcattcgc gagttgctcc aggacgagca ctacaacgtc
     8761 accaccacca acttcatccc caactccttt gagacgatcg agactgccca gccgtcgttg
     8821 ctgatcgtcg acctgatcct tggggagcag gcggggtggg acctgttggg caggctgcac
     8881 gaggcgatgt ccacacgcca tatcccggtg gtgctggtat cgaccacacc tcgattgtta
     8941 gaggaagcga aggagcgaca cgagctattt ggcggggatc gctacttggc caagcccttc
     9001 aacctggacg atctcttagg tcttatcact gaactgattg gcaaggcgta gtccagcgca
     9061 acaggagctg atgaaagacc cagctacgga gagcgcagat ctggagcctg aattaggttc
     9121 tggtcatgtt gctgtctcgg ggcgccagtc atctccgccc ggctttcctc tcgttccttt
     9181 actggctttc ttcacccctt cctgcgaatg tgtcctgact cccgggcaga tcacctgtga
     9241 tagctgatcc cggagaggtt gcctgtctct cgcgcgtggc cctttcagac gctatgatgg
     9301 cgcctgaagc ttttcagctc taaccggaag gcagttggtg cgccgtgagg aaggatgcaa
     9361 cgagcgatgg acgtcattgt atggaatgag ggtgagggag agattttggg gtctggtcct
     9421 tatcagttgc gttttctcat gcagtcaccc gatcagcccg tcgccatcac cgagaactcc
     9481 gtgccgcctc acttcccggg cccggtgagc caccgtcatg tgcgcatgac cgacatcttc
     9541 tacgtggtgg agggaacgtt gacgctccat tttgggagcg aacagcgccc gctcggggcc
     9601 ggaggctttg cgctcgtgcc acccggtgtg gtgcatacat tctccaacga ggaggatgta
     9661 cctgtccggt tcctcaatat ctaccagcca tccgggaatg agcactatct caaggaggtt
     9721 ggacagcgag tcgcggctgg ttctccccca tcaaaggcgg agatggcgga gattgccgca
     9781 cgctacgact tcgtgccggt tgttgacgag gagtgacctt ctccggccaa tgggcaacag
     9841 ccgtatgagc cccacctgct tcaagtcgtc cggatcatga tccgggctgc tcgcgatcgc
     9901 cggcgaatgc cgggatagcc gggcgtgctc cttcggttca aacgccgatc gtctccagtg
     9961 gccgcggtcg agcgacggtg ctgccgatct cgaggtgcct cccctgctcc gccgattcca
    10021 gcgcggcaag catgacatcc agggcgtgga agccgaggtc gccgctggcg cgggcgggct
    10081 ggccggtgcg gagggcatgg gcgagatcgg cgacgccgat gccgcgggtg tcggtgtcat
    10141 atccaggatc gagcggaaca tcctgccacg tcttttctcc acccctcagg agttggatcg
    10201 ggccgccgaa ggtgttcggg tctggcaggc ggatgctgcc ttccgagccg tagatggtga
    10261 atgcgcagtg ctccgggtcc cacaggtcaa aggtggttgt cagtgtgccg ataactccgc
    10321 tctccaactc caggttcgtc tcgaaatggg agggtgtgtt gacctcgatg acctggccat
    10381 atcttggctg gcttgtaatg gtccgccggg gaaaagtgat gcgggtcatc gcgctgacgc
    10441 gacggatcgg cccaagcagg gttgcgatgg cagtgacgta ataggggccc atgtcgagca
    10501 ttggccctgc tcccggctgg tagtagaagg ccggctcggg atgccagctt tcgtggccgt
    10561 gggtcgtcat gcgcgcgctg aaggcaaccg gctcgccgat ggccccctcg tcgatcaggc
    10621 gccggacagt ctggagcccg gcgccgagga aggtatccgg cgcacagcca acgaggacgc
    10681 cacgttgatg agcggcatgc agcagccgtt ccccctcccc gcggtcgagg gtaagcggtt
    10741 tttcggtgta gaccgatttt ccggcctcaa taaccccaaa gcagacagcg gcatgggcag
    10801 cggggatcgt caggttgagg acgatctcga tttcggggtg ggcaaggagc tcgtcgacgg
    10861 tgaaggcgtg gatgccaaac tgcccggctc gcgcctgcgc ccgatcggca ataagatcag
    10921 cgacaccaac gacacgaacg tgatcccacc tgctggtgag gttcttcagg tagatggcgg
    10981 agatgaaccc ggccccgatg accccgatgc cggtgggtcg gtcgttcatg gtgcatgccc
    11041 ctggatagat ccgttttgct gcggagcggc atcgggaggg gacgacgggt cactcccgcc
    11101 ctgcctgatg tccgcccctg catgagcacc gcggtgatgt gacaccgtgc gggcgaacat
    11161 tgagttcgcc cgcacggtgt ccctactccc cgccggcggg tgccgggcgg gcgacggtgc
    11221 tctcgaccgc gatgtgggtg ccgcgctcgg cggattccag gatcgcatgc atgacatcga
    11281 gcacgtgata cccaagctct ccactggcac gagccgggtg gccggtgcgc atcgacatcg
    11341 cgaggtccgc cgcgccaaga ccgcgggagt tctccgtgtg cggcttcgtc accgggatct
    11401 cctgccactc cttgctatcg cccacaagca gcttgatcgg gccaccaaag gtgttcgggt
    11461 cgggcaggcg caaggttccg tccgcgccgt agatcgtgag attcgcgaac tccgtctccc
    11521 agacatcgaa tgacgccgtt agcgatccca gaactccgct ctcgaactcc accgaggaga
    11581 cgatgtgcgt cggggtcgtg acagggatct tctcgccgag cttcgggccg ttgccaatgg
    11641 tccgctccgg gaagctggcg cgggcgaggg ccgagacgct ggcaaagggg ccgaggagcg
    11701 ccgcgagggc agtaatgtag tagacgccga catcgaagag ggggcctgcg ccaggctgga
    11761 agaagaagaa cgggtccggg tgccagtgct ccatgccatg cccgagcagc gcggcatgta
    11821 tcgccaccgg ctcgccgatg atcccctcgt cgagcagctg gcggaccgtc tgcaggccac
    11881 cgccgaggaa ggtgtcgggc gcggatccga cgtgcaaccc cttctcctgc gcgatctcga
    11941 gaatgcgctt gccttcctga cgggcgatcg ccagtggctt ctcgttgtag atcgacttgc
    12001 cggcctccag ggcgcggagc gcgatgtcgg catgggcggc cgggatcgta agattgacga
    12061 ccagctcgat ctttgggtgc gcgaggagct cttcaacggt cagcgcctca atcccgaact
    12121 ggtccgcgcg ggatttcgcg cgctcagcca gcacatcggc gacaccgatc accttcgtat
    12181 ggttgaagag cgtgctcaag ttcttcaggt agacctcgga gataacaccg gctccgacga
    12241 tcccgatgcc gaccggattg attgcgtcag acatgaacct cttcctgctc ctttattccg
    12301 ttgccccggc caatgtacgg gccgtggaaa tccgggtctc tcgcccgggg aatgtcctgg
    12361 cctatgatgc cgcccctgtg ggggcatgac aatcgcattc gcatgttcca gaccgttgac
    12421 gggcctgcga taatgctggc gattcgtgcc ggcctgtact gaaggatatc tgtgtgacac
    12481 tcaaactcat tcatgttgga cttggccgct ggggtatgga ctgggagacg accgcgctgg
    12541 aggcgatccg cgatcgggtg gagacggtcg ccgttgtgga ggctttcgag ccgacgctcg
    12601 agaaggcgaa ggcgaccctg aagctctccg acgaccagtg cttcgcgacg ctggacgacg
    12661 ccctgaagag cgtggagtcg gatgcggtgc tgatcaccgc gccaatggat gcgcatgtgc
    12721 cgctggcgat caaggcgatg cgggcggggc ggcatgtgct ggtggagaag ccgctggccg
    12781 gctcggtcga ggaggcgaaa gaggcggtcc aggtggcgga ggagaccggt aaggttctgc
    12841 aggtgagcca gaactatcgt ttctacccgg cggcgcgcaa ggcggcagag ctcgtgcaca
    12901 acgaggccct gggcgacctc ggcacgataa atattgactt ccggcaatgg gggaatgaag
    12961 ccgaagaggg aacaaacccg cattaccggt tcgtgcaccc actgatcttc gacatggcga
    13021 tccatcattt cgacctgttg cggctgattc tcaggcaaga accggtgcga atctacgtgc
    13081 agcagtcgga cccgcattgg agcaagtatg ttgaggaggc cgcggcgacg atggtgatca
    13141 ccttcgccga tggcacggtc gtgagctacc gcggctcttg ggtaagctcc gacctgcgga
    13201 cctactggac cggcgactgg cgaatggagt gcgagcaggg gctgatctac tggaccggtc
    13261 gccagggtgg cccgcacggc acggacggtg acaaggtgac catccgcacg cgtgacggga
    13321 aggtgaagga tgtcgcgctg gatgagatgc cgatctgggg ccgctctgcc ggtgtgctgg
    13381 cctttatcga ggcgatcgag accggcatcg agccggagaa ctccgctcgc cggaacttgg
    13441 gcagcattgc cctgatggaa gcggcggcga aatcgatggc ttctggccag gtcgaggatg
    13501 tcgaggtggt gtcgtaaccg cgacgcttcc tgccttcacg atgcgggtct acggtgccgg
    13561 ctggcgtagg agatcggagg gacctcctac gccagatcgc gggcagtgag cgagtgccgg
    13621 tccctatcca cagaaccgca cgacgaggtg gtagatgaaa cgggtgccga acccgaggtc
    13681 gtcgatgtgg agacgctcat tatggccgtg caccagcgtg tcgtagagcg ggatgcgctc
    13741 cgttggcagg gccgggaaga agccatagac cttcacgcca ggcaccagtc cggcgtccgt
    13801 tccgccggag atcagggtcg ggacgaccgt cgcgtccggc cgcagggcgg acatcgtctc
    13861 cttgatcgcc tcgaagaatg cggattccgg atcagcctcg atgccggatt cctgggtctc
    13921 atagaggaac gtgatctcgg cgacatcccc aacagcctcc tgcacggcgg cacggaaggc
    13981 ctccggctct tccccgggga ggatgcgccc gtcgatctcc accacaacct ctgacggaat
    14041 gacgttgatc tgcctgccgc cgtggatgat cgtcgggacg gcggtgttgc gggtgatggc
    14101 ccggaagtgt gccttctcgt tctctggcag cggcagtttc gccagctcgg cccaggaggg
    14161 agagccctgc tccaggagcg agcggatgga gtcgcccgtg tcgctgccaa gcgcaccggc
    14221 aatgccctcc agcatgagcc ggacggaggc ggtgatctgg gtcgggggct cccagtcgtg
    14281 cagccggtgc agcgcctcgg cgagcttctc catggcggta ttgctctgcg gcaccgacgc
    14341 atggcccggc tcgccgcgaa cggtcatctt cagccgggtg ccgcccttct cccccacctg
    14401 gcaggtatag aagagatgat tcgcgatctc gatcgggctg ccgcctccct cattgatcgc
    14461 gaactcggcg tcgatcagct cctggtggtg cttccataca tagtcggcgc cgaacgcacc
    14521 accggcttct tcatcggcaa aggcgacgaa gatcaggtcg cgattgagcg ggatgccgga
    14581 tcgcttgatg tgcaggaacg cggccaattc ggcggccacc tgccccttca tgtcgaccgc
    14641 accacgcccc cagacgaacc cgtcaatcaa ttccccgccg aagggatcgc gatcccattt
    14701 gtcgcgctcg acggtcacga catcgacgtg ccccatcagc aggaccgggc gcgcggtcgg
    14761 gttttccgcc tccagccggg caacgacgct gacacgtccc ggtgccgcct ccacgaaggt
    14821 gcagtcaatg tcctcctcgg cgagcgcccc cttgatgtac tcggcggcga tgcgctcatt
    14881 gccgggaggg ctctgcgtat cgcagcggag caggttctgg aggtggaaga cggcggcatt
    14941 ctggaggttt cgggcaacgt ccgtggtggc gatatcggcc atgaaaagat ctctcctcgc
    15001 gggtggatag tggtatcggg cgcagttgcg ctccgtggat cgtacgaggt ggacacctat
    15061 cgggcaatcc cccggccgat ctggcacaga tgccggaggc agaggctgat gtggttctcg
    15121 ctgacagggc agagggtgcg ctatggtagg ctggtgtgtg gcctcagttg ccgccatttg
    15181 agcgcgtcgg cgctctccat gcgatgttct ccacgcgcca cgaccgcaca atggacctca
    15241 ggaatgacta tcaactcctc cccttcttcg accaggaaaa gcacacggct tgtgctgatc
    15301 gctgccgtga ccgctctcgc tgtcctggcg attgccggag cggcgtggtt cttctacttg
    15361 cggcccgagc cggcatacgc cttccatggc gggttctacg agccgccaaa tccggcaccc
    15421 tcattggacg gggcggtcga tcagcacggg aatcccttca agctggagga ctaccgcggc
    15481 aagctcgtgt tcgtctattt cggatacacc cactgcccgg acgcctgccc ggcaacgctc
    15541 aacgaattct tggaagtcaa ggacgcgctt ggtgaggacg cggaggatgt cgccttcgtg
    15601 atgatcacgg tcgatcccgc gcgcgacacg cccccacagc tagccgagtt cctggcctcc
    15661 tgggatccgg cgttctacgg cgtctcgatg ccggaaacgg agacggaggc ggtcgctggc
    15721 gcctggaacg ttcactacaa tcaccatcat gcggatcccg gggatggata tctggtcgac
    15781 cacgaggtct cctcgttcgt cgttgacaag gatggcaaca tccgcctcat ctacccgctc
    15841 ggcttcgaca ccgggttgat ggcggaagac gccgagtacc tgctgggcga gtagcgctgc
    15901 gtccgatcaa cggaccgtgg agcttccaac cctgcccggc ggatcgggag cgaatggacc
    15961 agcgcgggga cgaacggcga tgtccgactc gtctgccttg atgggaaggg aagcgagatg
    16021 agcgtcatga tctggaacgg gatgacgcgc gaggcactcg ggtcggcgtt gccggattcg
    16081 gtggtcgtct tgccgaccgc cgcgacggag cagcacggcc cgcacctggc gaccggccat
    16141 gatgccctcg tgctcgacga gatcgtccgc cgggcggctg ggcaggttga tgtccggacg
    16201 aatgttgtcg tggcgccgac gctggcattc ggctcctcag atcatcacct tccctttggc
    16261 ggcacgctct cgctccgcag tgaaacctac ctggccgtgg tcaaggatct cctgagatcg
    16321 atgatcgaga gcggtgcccg acgggtgctc gtggtgaatg gacatggcgg gaatcatgag
    16381 ctgaacgaga tcgccgtgcg ggatgtcgcg ctggtccagc cggcggaaca gccagtcgtg
    16441 cttgcggctg gctcatggtg ggcgatcacc gccaaagcac tggcggggcg ggaggagttt
    16501 gggggcgtgc gtgttcccgg gcatgccggg cagctggaga ccgcgatgat gctggcactg
    16561 caacccgagc tggtggagga tgggcgtccc gcacgcgagc acgatccgtc actgggaacg
    16621 cggattgccg gacttcgtgt tgaaggcgaa gtgccctggc gacagtttga cgggttcacc
    16681 gactcccctg ccctcgcgac gccggcgctt ggtgcactcg ccctggatct tgccgccggc
    16741 gccttggccc aggcgattac tcgggttgca gatgctggaa ctgcggcggc ctgatggcac
    16801 agtgagccgc attccgagtt ggtgaagcga gcagacatgc tggcttcagg ggccagtgca
    16861 atcaagagcg ccattctcct tgagatgatg cagggacagt catgctgcat tctcgagaga
    16921 atggcgccga tagcgggcca ggtgttaatc ggccttggga aaacatttcg aggccgatac
    16981 gacgcctcat tgactcattc gttcccggtg ccgagcagtt ctacatttgt ccgtgagccg
    17041 aaataggttt tgtagggcat gagcgccacg gctcgcttaa agcctgcctc cgcgccgcgc
    17101 atctcgctcg cccctcggta ccattgctcg tactggaacg gaaccacttc tcctcggaag
    17161 aggctgtagg cgtggatggc ctcgatgtac cgatcaaaga cgtcggtggt atcgagatat
    17221 atctccggcc ggaagtcttc gacgtcttcc caattctccg cgaagtagat cttctcgggc
    17281 cggtggggcg cgtactcacg cacgaacgtc ctgagccccg caaagaacag ggacgcgagg
    17341 acgttgtagt aggtgttgtt gtggtcccgg tggaagctgc ccttccagtg cgtgaggatg
    17401 ctgttcggac ggtattgccg gatgacgtcc gcgacggcca tctggacctc ttcattgacc
    17461 agcagctcac cgtcggccca cggcatcatg accgattctg ccccaaggag tttcgcggcg
    17521 ttcagggctt cttcctcttt aatagtgagg tactcctcgg gagtgagctt caggtgcccc
    17581 ttttcacctc ccgtcatgtg gaccatcacc gctctccagc cagcgtccac atgctaaagg
    17641 acggtggcac cgcccatgat ttccgcatcg gcagcgtggg cgccgatgac catgatgcat
    17701 ttccgggtct cttctgaaga catgtttcac gctcccctca atcgaatggc tttctgtctc
    17761 gcgtaacctg gcgacaggaa tacaggcgac tgcatcctgt ggctcatctt caaacgttcc
    17821 tactgccccg agatatcctt cctcatcacc gcaaatctcc gcacctgatg aaacccgagt
    17881 tgaccgtaca accgggcggc gtcatcacct gtccacagga accatgccgc gtggaagttc
    17941 ttctgcagca tcccggcgag ggtctccgcc agcaggacac ggccgatgcc gcggctgcgc
    18001 aggtccggcc gtacgccgaa cggtccaaac cgctcgccgc gatgctgggc gtagccgagg
    18061 acctcgccgt cctggcgggc gacaatcatg ccgacaaacc gggggtcgcc gttgtagagg
    18121 tcgttgaaga cgccggtcgc ctcccggtgc cagtcccagg tgaaatgacg cgggatgaaa
    18181 tcgagcacgg gtgcaatatc ggccggggtc accagccgga cctcgacgcc ctcagccgcc
    18241 agcgcctgct gtcgctcccg gacgggctcc ggcgtcgtga acccggtcag gtccgcgcgc
    18301 atgctgatcg ggcgctcgat gacctcgaaa ccctcccgcg tcaggaagtc gatgccgggc
    18361 ccataggtcg cgacatcggc gccgggggtg aagtaattgg ggacataggg tgcgagcgcc
    18421 accgacgtgc ggcccagcga ccgtaagcgg tcgatcgccg cgtgcagcag ggcgcgaccg
    18481 attccctggc gctgccatgc cgggtcgacg gcgaaggccg tgatccagga ttgctccggc
    18541 tcgagcccgt cattgaagaa cggaacgcgc cgcaccagcg acaagaggaa gccgcgcacc
    18601 tccccattca tctcggcgac gaggcatccc tcgcggtcga aattggggtc gagcagcacc
    18661 ttgccgcgcc acctcgtcgc attgatcggg tcggcataca gcccgctgtt ccatgtgcgc
    18721 agcaggcccg cttcatctgt ttctgcatac gcccgaatgg tgaaatccgc catggccatc
    18781 tcagcccttc atccctgtca gtgcgatacc tcgcacgaaa taccgttgga gcagcaggaa
    18841 cacgatcatg acgggcacga tcgcgagcgc cgccccggcc atctgcaagt tgatcacctc
    18901 ggcgctgctt gcgtaaacgc ggaggctgtt gagcccgacg ggcaccgtcc acatatcgga
    18961 cgagcgcgcc acgaccagcg gccacaggaa gttgttccac tggtacacga aggcgaggat
    19021 tcccaatgcc ccgatcaccg gcgtgctcag cggcaaggcg attcgccagt agatctcgaa
    19081 ctccgacgcc ccgtcgatcc ttgccgcatc gaggacttcg tccgggaggc tcagcatgta
    19141 ctggcgcatc aggaagatgc cgaagccggt cacgatattg ggcacgatca gcccctgata
    19201 ggtattgatc caccccagct ggctcaccat atagaagatc ggcaggagga cgacaaagaa
    19261 cgggatcatc atggttgcca ggaccaggag gaagaggaaa tcccggcccg cgaactggaa
    19321 cttggcgaac ccgtagccgg ccagcgagct ggtgagcagg atgccaaggg tggtcagcgt
    19381 cgtgacgatg atgctgttga cgtacatccg accgaggggg atcgcgtccg atagcgcctc
    19441 atagttctcg aaccgcaacg cggaaggaat ccaggtcagc ggccgggcga tcacctcctg
    19501 cgtcgacatg agcgaggacg agagcatcca ggcaaacggc aggagcatca cgaacgcgcc
    19561 gagcgccagg atgccgtagg tgacgacggg cttgaaaaac gccgcgaatt tcatggaagt
    19621 actcatttac gttcccgcaa gagacgaagc tggaccagcg tgatcacgaa gatgacgagg
    19681 aagagcaaca ccgccatggc agttgcccgc tcgacccgca ggaagctaaa cgccgtctca
    19741 tacatgtaaa gcaccagcct gttggtcgca ttcaccggcc ctccctgcgt gaggatgaat
    19801 ggcaggtcaa agatctggag cgagttcagg gtgttcacca cgacgacgaa cagcatcgtc
    19861 ggccgcagca acggcagggt gatcgaccag aacagacgcc acgcattcgc gccgtcgagc
    19921 ttcgccgcct cgtagaggtc cccggggatc acctgcaacc cggcgaggaa gatgatcatg
    19981 tagtacccaa tgccctgcca gaccgcgacg ccgatcacac tgggcagcgc ctggctcacg
    20041 ctgtcgagga atggttgctg gggcagaccc atctggttga ggaggccgtt gagcatgcca
    20101 tatcgatcat tgaggatgct gcgccagacg atcgcgatgg ccaccggtac ggtgacgtaa
    20161 gggatgaaga acgccgcccg ccagaacggc cggtatcgaa cgggcccgtt caggatcagc
    20221 gcgatgcaca gcgcgaagac gaccgagatc ccaacattcg cgagcgcgaa gataaccgtg
    20281 gtgcggaagg tttcctggaa cagtggatcg tcccggagga ggtactggta attctccagc
    20341 ccgcggaaag gcatatcccc gacgggcgtg agcatgttcc actgatgcag gctcacccag
    20401 atcgtgaaca ggaacggggc gagcgagaac gcaaggagga agagcaccac cggcaggaca
    20461 aacatatatc cggcaaacgc tttccggccg atcagcctgc gcctcctggt tgtgatggat
    20521 gtggtggccg gcaagactgc cgggaatcgc tgttgcatgt cagccttttc actggcgtgt
    20581 caagatcaag gggagcat
//
LOCUS       DASYWM010000148        14640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_99558, whole genome shotgun sequence.
ACCESSION   DASYWM010000148 DASYWM010000000
VERSION     DASYWM010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14640
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99558"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1344
                     /locus_tag="VGR29_06035"
     CDS             367..1344
                     /locus_tag="VGR29_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2073186.1"
                     /translation="MVHPNEHTDRRTPEKVADGTHLIDLGFQGVPGAIGSFLLAGDND
                     LVLVESGPSTAVWNLVQGVRDAGFDLSDVSRIIVTHIHLDHAGAAGLLMKEYPGIRLS
                     VHPSGAPHLIDPERLLKSATRIFGDRMDRLWGDVAGADADRVDPMVDGDVLNVAGREV
                     LVKAAPGHAGSHVVLLDRSSGVLFTGDAAGARLKGTEYVCPTLVPPELDVALWAETVE
                     MMQGLGASKLALTHCGVFEDVDRHLGDVMPNIEEQVTIGEQVMTSPNDEERVIEFLTV
                     QEREEYAREGEDIARVNAHMRAMGLAMPPYIAAQGLKRLFTKAGKFDQA"
     gene            complement(1433..1951)
                     /locus_tag="VGR29_06040"
     CDS             complement(1433..1951)
                     /locus_tag="VGR29_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HEV2073187.1"
                     /translation="MHHNSYDTDWETFDDLPTSDDFHAAERRRRSRRSSGNIDGRRKR
                     NRLTRTPRSDADQAFKCGHCRQFIGAPISGGRHRNHCPNCLYSRHVDKSHPGDRRSDC
                     HALMQPAGLLARRNGEQVIIHRCLGCDKEDPNRIAADDNAILLMRLQLVQPAGLMLVP
                     TNTAEPTEEESA"
     gene            complement(2316..2444)
                     /locus_tag="VGR29_06045"
     CDS             complement(2316..2444)
                     /locus_tag="VGR29_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073188.1"
                     /translation="MSSVNSLHPRLSTLERQALMLHHLILVPEGVTLRDARIAASL"
     gene            2527..3531
                     /locus_tag="VGR29_06050"
     CDS             2527..3531
                     /locus_tag="VGR29_06050"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV2073189.1"
                     /translation="MMDVLLLGNGAMVPLPDRWLSSVLVRLDGSLTLLDCGEGTQVSW
                     RRFHWGFKRLDTICLTHHHADHVAGLPGLFHTVSNAGRTQPMHIYGPPGTIEVIKGLR
                     VIAQWLAFDMIVQELEGGDTFELPSGVQGRVAWGDHRIPVLAYRFDLPRAPGFLPGRA
                     ESLGIPRTKWKLLQRGERVEVDGRIVEPGEVLTESRRGISLGMVTDTRPTDDIRKLMH
                     GVDLLISEGTYGDDAEEEKAISHKHMTFREAATLAHEAQAGALWLTHFGAGMENPRRW
                     ERNARDVFTNVELGDAGLTGRISFDEGYVPAGLVDRDTGSSPESADSINAEIAPRSPM
                     "
     gene            complement(3435..4655)
                     /locus_tag="VGR29_06055"
     CDS             complement(3435..4655)
                     /locus_tag="VGR29_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV2073190.1"
                     /translation="MQPRPQNLVPVQRPVTATPREYRRAHWSKRRKIAIGIALLIVFA
                     PIVTIGWYAWSITNAIGDAQDVAVVDFPERREPITDGPGNQQPALTGSPVPRNAPNDD
                     PSSLEVARGLISARTGGENTSPSAVWPDERYLNILVVGVDTRPKGGDQNADVIIIARL
                     DLEAKTMNSVSLPRDLLVEIPGHGEGKINGAYGIGVNADPESRVAGVAKLRETIEHNF
                     DIRIDDYVMVDFEGFKEVVDAVGGIDINVPTRIVDEEYPTEDYGTRTLVVEAGPQHMD
                     GEMALAYARTRHADSDDARRERQMLVIRALFEKGRELGTITRVADLITALSDAAQTSF
                     HWNEQLALAALALNMDESRIQMSNLEQPLIQPGTSSTGAWVYIGDLGAISAFIESALS
                     GEEPVSRSTRPAGT"
     gene            4897..6153
                     /locus_tag="VGR29_06060"
     CDS             4897..6153
                     /locus_tag="VGR29_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV2073191.1"
                     /translation="MKREKTDWFVHDRFGLFVHWGIYAVPGRHEWVQSREKIAAEDYK
                     KYADRFEPDLYDPVQWALDAKRTGMKYAVLTSKHHDGFAMWDSQLTDFKVTNTPAGRD
                     LLGEFVEAFRAEGLKIGFYHSVIDWHHPDYTLDSPHPNRDQNPDEFNQGRDMGRYREF
                     LHGQVRELLTNYGKIDVMWFDWTPPEKDRKDWDSENLSAIVRELQPGILLNDRIDYPE
                     SADFVTPEEYQAKAWPERDGKRVLWETCQTLNGSWGYDRDNVNWKSPEVLVKLLVDTV
                     SKGGNLLLNVGPTARGEFDPTARETLAAIGEWIRRHGRSIYGATAAEECPPQDCRFTK
                     RGNRLYLHVCSWPLRHIHLEGFAGRVDYAQFLHDGSEIFFRDGDPNAVAQNTAMAEDA
                     GTVILEVPFRQPDVMVPVIELFLSDS"
     gene            complement(6317..8671)
                     /locus_tag="VGR29_06065"
     CDS             complement(6317..8671)
                     /locus_tag="VGR29_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEV2073192.1"
                     /translation="MDWFDVTLRWYLVTTLVTIGLAPFTVLVFRRMTDRGASVVRPLS
                     ALFLIWPVWFLASIGDAIVPFTAVTLWISALVLGATAWGLGWREGVLNANTFRHLLIA
                     EAGYGVAFGFFLWFRGYGPAANLQEKPSDLMMLASSMRSTSMPPADAWLAGEPVNYYY
                     LGYAIWAAFGNMIGTSPAIVFNLALASVFAMTFVAVVGLAANILSRWYSNLVARIGGF
                     IAALLLVIVGNPWAASRVLEAAKSQWNAWFFDGIGWQSTRIIVDDPESGANPISEFPS
                     FSFILGDLHPHVLALPYTVTALMFAWMLLTLGRLRENESLLRRDGGRLVTAGFAIGSL
                     YAMNSWDFPTYLLIALLALALGTVGLAVQDRLLGGGLLVFTALVAWIPFYVTFEAPAR
                     STGTPFSEWIGNVPLLGGILASVASYQGERTSPQDYFSIFGFMYFIALTLILTETWQR
                     REDVLIARAEARGVTWERDQAGHYFAIVTALLCFLGSLIVPAPLLVICGLPVIVVWLL
                     LERDFRVTPVNIALVMYAIALIMTLVPEFFYVTDYYGGSRMNTIFKIYYQVWLLMAVA
                     SALAAVSIWKAFRRNLVARYALPAVMTVILALGMTYPVVSGHQWLEWRAPDREWKGVD
                     GLAYLLEEQNGLYTGEYEAIQWLLDNAEEQDVLLAAGGGEWREQLGRVSSGSGVPTLI
                     GWTGHENQWHLGDPDFQSTVNQRLEDINVLYSGPPSTDVLDRYGVTLIYVGPTETRGI
                     SEQSQSGWVAPGPFPAVSDPKYPGDGWTEVFNEDGSRIFRREDT"
     gene            complement(8938..9573)
                     /locus_tag="VGR29_06070"
     CDS             complement(8938..9573)
                     /locus_tag="VGR29_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2073193.1"
                     /translation="MISVLLVDDHPVVIEGLRKLLDVAGDIDVTGEAHDASSAITQAK
                     LLQPDVILLDLRMPGATGIQTARRLREQEYEGAIIVLTSYGDQAYVTQALEAGADGYL
                     LKSTPSAQLISSIRDASRGRRQLSPELLNGVLEQFGGLAREKTARDADLSEDDREILR
                     RASQGATNREIGIAMNRSEIAIKKRLQSIFQKLGAVDRAHAVAEAIRRGLI"
     gene            complement(9586..11607)
                     /locus_tag="VGR29_06075"
     CDS             complement(9586..11607)
                     /locus_tag="VGR29_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV2073194.1"
                     /translation="MFRHTSVRTRFILFALISLIPLLIVIIFFLDRSAEQSNQEIITG
                     EATISTLVTQSLANYINSRYQTIERLSGVPVIQQQDNIDAVNHVLGEARALRPEMSGI
                     FLINTGEQVVGESGTDAELVLPDIRNQIETTLTTGQRTISGRIELADETNVIVLLAPV
                     TTITQTQTTAAAPGDAPTPTPPSDDNGRERQATTTVTPEATPGQQPPGTILGVVGAVV
                     QIDTMTQSVIPVPRTRADIAIVSSDEVIAATGDIRQNEEDFLRRIDEEDIRAGSSGTD
                     VFQMTSTSGMERLATFAPVPLEFADWAVIVSFPVPRPYGDSLWLEGALMLALATIAVL
                     AIAVAIGEYTARPLRDLARDSQLLQQGDYSVSIQPNGGGEILSLSTALSELGNRLGGQ
                     VAGLEQSHLERQRQTDQMRELLRRTLRLQEDERRRIAGEIHDAVSPLITGALYQARAL
                     QMANGSSTHETLNETLQSVNSLLERASEELHGVIFDLRPPDLDDIGVVAALEAYVSTI
                     QRTGLQARLEVINDLPPLTPEVRLGIYRIVQEALHNVVRHASADEAVVRIEFASDLLR
                     VTIRDNGAGFTPGSAVRPTSLGMLSMRERAAAIGATFSIISKPGGGTAIVIERTETGN
                     VMSDEILDDLIRHRDDESQVVDVVHSSDNTGNEDASGTSETDDEGRRDS"
     gene            12041..13180
                     /locus_tag="VGR29_06080"
     CDS             12041..13180
                     /locus_tag="VGR29_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2073195.1"
                     /translation="MSKVVVVGGGIVGASAAFHLAREGVETVLVDRRDAGRATSAGAG
                     IVAPGSSLRDSPGFYHLALPAVAHYPELVAALGEVDAGETGYQVCGKIFLAETDEQAW
                     DLDNVLALFNERRANGMPNLGEIQDIDEREAQRLFPALGVVPRALYIPEAARVDGALM
                     RDALTTGARHYGAQVMLGAAKPVMDQGRVIGISCDGLLIEADAVILAAGAWSKEMFEP
                     LGFSLPVAPQKGQILHIRLEGQETTDWPILSWFGDQYILAFGPDRVVAGATREFGSGF
                     DTRITPGGVKHVLDTALRIAPGLANGTLAEVRVGLRPYAEDSLPFMGMAPGHGNVVVA
                     TGHGPSGLQLGPYSGYLAAELVMGRTPPIDLTPFAVDREILASQG"
     gene            13188..14048
                     /locus_tag="VGR29_06085"
     CDS             13188..14048
                     /locus_tag="VGR29_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2073196.1"
                     /translation="MTTPQQGITFGIVTGQHQLTWDQIRDMWLLGESLGFESLYLFDH
                     FTGLYGDPAGPCLEASTLLAALALETEHADIGVLVYGNTHRHPFVLAKEIVTVDHLSK
                     GRAVLGIGTGWNENEHRWYGIPLHSPGTRVAMLDEALTVIDGLLANERFTFAGQHYQL
                     DDAPFAPKPVREKMPILVGGKKPKMLRVVARHADIWDASGTPEVRKERSEELRRACDE
                     VGRDYHEIRHSMSFGADKLVDIDHFEELVTAYKGVGVQQFLFDFPLDKDGQQIARKVA
                     TELMPKLRLA"
     gene            14108..>14640
                     /locus_tag="VGR29_06090"
     CDS             14108..>14640
                     /locus_tag="VGR29_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2073197.1"
                     /translation="MQISEMIHRRIELVTFDLYDTLVEANPPRWERFAVALERSGMPI
                     DVEAVLHADRKGEDHYTIENGRYPIRDRTDKEVQQFRLDQAAAYLHALDLPTDPETVR
                     TVQEHFRDETQNHGGWSYRAFEEIPKVMECLEQHGIKRAIISNADADVTRFCLQMGFA
                     AHMDVIVTSALVGWEKP"
BASE COUNT         2947 a         4095 c         4695 g         2903 t
ORIGIN      
        1 tggcctccca cgcggcggcc agggagacgc ccttcccgaa ggtagcggcg cacctgctcg
       61 gtggagcgtt tcaacaagcg ggaggcatcc gccacgctca cgagatccct gtcggaactg
      121 ctacgatcca taatcggaca cctgctgact gaggcagaga cccgatttga gagtctatct
      181 gccgagaagg cgctgtctta ccagcatcgg aatgatgctg accgcggcgc gtgcgtcgtg
      241 tgcaacaaca tggtatccag ttcttgctct ttggggcaag gggccggtcc tgaccgattc
      301 gcctcgccgt tggattacca tgcttccaac ggatttgccg ggggactgac ggagaagacg
      361 agaatcatgg tgcatcccaa cgaacacact gatcggcgaa ctccggagaa ggttgccgat
      421 gggacccacc tgattgatct tgggttccag ggcgtgccgg gtgcaatcgg atcgttcttg
      481 ctggcaggtg acaacgatct tgtgctggtc gaatcaggcc cgtccaccgc tgtttggaat
      541 ttagtccagg gagtaaggga cgcaggattt gatctgtcgg acgtgtcgag aatcatcgtc
      601 acacatattc atctcgatca cgcaggtgcc gctggcctcc tcatgaagga gtatcccggc
      661 atcaggcttt cggtacaccc cagtggagcg ccgcacctta tcgatcccga gcgcctgctc
      721 aagagtgcga cgcgcatctt cggggatcgc atggatcggt tgtggggcga tgtcgctggt
      781 gcggatgccg atcgtgtcga ccccatggtg gatggggatg tcctgaatgt ggcgggacgg
      841 gaggtgcttg tgaaggctgc accgggtcat gccggctcgc acgttgtatt gctggatcgg
      901 tctagtggag tcttgtttac gggcgatgcc gccggagcac gcctgaaggg tacagagtac
      961 gtctgcccca ccctggtccc accggagctc gatgtggcgc tctgggcgga gacggtcgag
     1021 atgatgcagg ggctcggtgc atccaaactg gcgctcacgc attgcggggt gtttgaggat
     1081 gtggaccgac acctgggtga tgtgatgccg aacatcgagg agcaagtcac gatcggcgag
     1141 caggtgatga cctcacccaa cgacgaggag agggtcatcg agttcctgac ggtgcaggag
     1201 cgtgaggagt acgcccgcga aggagaggat atcgccaggg taaacgccca catgcgagcc
     1261 atgggactgg caatgccacc ctatatcgca gcacaggggc tgaagcggct cttcacgaag
     1321 gctggcaagt tcgaccaggc ttgacttgac tatgcaatcc tgcgctggtt ggtaaaggct
     1381 ttcatctgcc acgcggaacg gtcccgatcc agcctggatt gggaccgttt acttacgcgg
     1441 attcttcttc cgtcggctcg gcggtattgg tcgggacgag catgagtcct gccggctgaa
     1501 ccagttggag ccgcatcagc aggatcgcat tatcgtccgc agcgatgcgg ttcggatctt
     1561 ccttgtcgca cccgaggcag cggtgaatga tcacctgctc cccgttcctg cgggcgagca
     1621 acccggcagg ctgcatcagc gcatggcagt cgctccggcg gtcgccgggg tggctcttgt
     1681 cgacgtgccg cgagtagagg cagtttgggc aatgattacg atgccgtccg ccactgatcg
     1741 gcgccccgat gaattgccgg caatgtccgc atttgaacgc ctggtccgcg tcgcttcgag
     1801 gagtacgggt gagccggttt cgcttccggc gaccatcgat gttcccggag cttcgccggc
     1861 ttcgcctgcg tcgctcggca gcatgaaaat cgtcactggt cgggaggtcg tcgaatgtct
     1921 cccagtctgt gtcgtatgag ttgtgatgca atgatgaacc ctccgcgccg gcggcgcgtg
     1981 tgaggtggat tggtcatggt cgggttcctc tgtccgttgg cggcaactgc ggccattgcg
     2041 gacccatgta caagggcttg tgtcgacata gaggccttct ttccagcggg catcttcgtg
     2101 ccctggcaag tcgtcgagtt ggatgtgatg cgcacgagga catcacaaaa atcccgtgtg
     2161 atcgaagcga tcagcggtga ggccgagagg ggatgtcagg ccgtggaccg tcggggtacg
     2221 gtacgtgccc cacatgttct gtgggtgcga cgtcactccc tgcgcgagct gcaaggcagc
     2281 gtcgcgggat cgttgactat gttgcaggga ggcccctaca gggaggcagc gatccgggcg
     2341 tcacggaggg tgacaccttc tggcacaagg atcaggtgat gcagcatcaa ggcctgcctt
     2401 tccagcgtcg agagacgtgg gtgaagcgaa ttgacagagg acatctccat cgtcgattac
     2461 gcactataaa cgcagtctag ggccgaagtt atcaaaacac aagcacttgc aggaggaacc
     2521 taggacatga tggatgtgtt gctgctgggg aatggcgcga tggtgccgct gccggaccgc
     2581 tggctgtcca gtgtcctcgt gcgacttgat ggatcactga cgctgctgga ttgcggtgaa
     2641 gggacgcaag tcagctggcg tcgttttcat tggggattca agcggctgga tacgatctgc
     2701 ctcacacatc atcatgccga ccatgttgcc gggcttcccg ggctctttca caccgtgtct
     2761 aacgccggtc ggacgcagcc gatgcacatc tacgggccac cgggaacgat cgaggttatc
     2821 aagggtttgc gcgtaatcgc gcaatggctg gcgttcgata tgatcgtcca ggagctggaa
     2881 ggtggagaca cgtttgagct gccatctggc gtccagggac gggtcgcgtg gggggatcat
     2941 cgcatcccgg tgctggccta ccggttcgac ctgccacggg cgccaggatt cctgcccggc
     3001 cgtgccgaat cgctggggat ccctagaacg aagtggaaac tcctccagcg aggggagcgg
     3061 gtcgaggtgg atggcaggat cgtcgagccg ggcgaggtgt tgacggaaag ccggcgtggt
     3121 atcagtctcg gaatggtcac ggatactcgg ccgaccgacg acatacggaa gctgatgcac
     3181 ggtgtcgatc tgctcatcag cgaagggacc tatggcgatg atgccgagga ggagaaggcc
     3241 atttcacaca agcacatgac cttccgggaa gcggcaacat tggcgcatga ggctcaggct
     3301 ggggccctct ggctcacgca ctttggcgcc gggatggaga atccccgccg gtgggagcga
     3361 aacgcgcggg acgtattcac caatgtggaa ctcggggacg ccggtctgac gggacggatc
     3421 tcatttgacg agggctacgt gccggcaggt ctcgtcgacc gcgacacggg ttcttccccc
     3481 gagagcgccg attcgataaa tgcggagatc gctccaagat caccgatgta gacccatgct
     3541 ccggtgctgg aggttcccgg ttggatcaac ggctgttcga ggttcgacat ttggatacgg
     3601 gattcgtcca tattgagggc aagagccgcc agggctagtt gctcgttcca gtggaagctg
     3661 gtttgggcgg catcactgag cgccgtgatc agatcggcca cacgggtgat ggtccccagt
     3721 tcccggccct tctcgaagag tgcccggatg acgagcatct ggcgctcgcg ccgggcgtcg
     3781 tcgctgtcgg cgtggcgggt acgggcgtac gcgagcgcca tttccccatc catgtgctga
     3841 ggtccggcct ccaccaccaa cgtgcgggtt ccgtaatcct cagtgggata ttcctcgtcc
     3901 acgatgcggg tggggacgtt aatgtcgatc ccaccgaccg cgtccactac ctccttaaag
     3961 ccctcgaagt cgaccatgac atagtcatcg atgcggatgt cgaagttgtg ctcgatggtt
     4021 tcccgcagtt tggcaacgcc ggccacccgg ctttcaggat ctgcattcac cccaattcca
     4081 taggccccgt tgatcttccc ctccccatgg cccgggatct ccaccagcag gtctcgtggg
     4141 agactgacag agttcatcgt ctttgcttcg aggtccaggc gcgcgatgat gatcacgtcg
     4201 gcgttttgat cccctccctt gggacgggtg tcgacaccaa cgaccaggat gttgaggtag
     4261 cgctcgtccg gccagactgc cgagggggag gtgttttcac cgccggttcg ggccgagatt
     4321 agtccacgtg ccacctcaag tgacgaggga tcgtcattcg gtgcattcct gggtactggg
     4381 cttccagtaa gtgccggctg ctggtttcct ggcccgtcgg tgatcggttc ccgccgctcg
     4441 gggaagtcga ccacggcaac atcctgcgca tcgccgattg cgttggtgat cgaccaggcg
     4501 taccagccta tggtgacgat gggagcgaac acgatgagca gcgcgatgcc aatggcgatc
     4561 ttgcgacgct ttgaccaatg cgcgcggcgg tattcccggg gcgtggcggt cactggtcgc
     4621 tgtacgggga ccaggttctg ggggcgaggt tgcattcagg cgatccttac aggcacagtc
     4681 tgttcacggg atgtgatgtc cgacgtcggc attggttttg aatgaggatc cctgataacg
     4741 cgcctgtcgc attctcgtgc aaagtgtgcg ttgcgacctg atcatctgca agttttgcat
     4801 gtacccctac tacaaaaggg ctgaatcgtc tagtcgtagg gtgccgggcg ggcgcatgta
     4861 aggcgagata cgggcccgga agggaggaga tgcgacgtga aacgggaaaa gactgactgg
     4921 tttgtccacg accgcttcgg cctttttgtg cactggggca tttatgccgt accgggacga
     4981 cacgagtggg tacagagccg ggagaagatc gcggccgagg actacaagaa gtacgctgat
     5041 cgattcgagc ctgacctcta tgatccagtg caatgggcgc tagacgcgaa gcgaaccggc
     5101 atgaagtacg cagtcctgac gtccaaacac catgacggtt tcgcgatgtg ggattcgcaa
     5161 cttaccgact tcaaggtgac aaacacaccg gcggggcgcg acctgctggg agagttcgtg
     5221 gaagcattca gggccgaggg cctgaagatc ggcttctacc actctgtcat cgactggcat
     5281 catccggact acacgctgga ttcgcctcac cctaatcgcg accagaatcc ggacgagttt
     5341 aaccagggcc gggatatggg ccgctacagg gagttcttgc atggccaggt gcgggagttg
     5401 ctgacgaatt acgggaagat cgacgtgatg tggttcgact ggaccccgcc cgaaaaagac
     5461 cggaaggact gggactcaga gaacctgtcg gcaatcgttc gcgagctcca gcccggcatt
     5521 ctgctgaacg atcgcatcga ttacccggag tcggcggatt tcgtcacacc tgaggagtat
     5581 caggcaaaag cctggccgga acgtgacggc aagcgggtgc tctgggaaac gtgccagacg
     5641 ctcaacggaa gttggggata cgatcgggac aatgtgaact ggaagtcacc ggaggtgctg
     5701 gtcaaacttc tggtcgacac ggtctccaaa ggtgggaacc tgctgctcaa cgtcggacca
     5761 acggccaggg gcgagttcga cccgaccgca agagagaccc tcgccgcgat tggcgaatgg
     5821 atacgccggc atgggaggtc aatctatggg gcgactgcgg cagaggagtg cccaccgcag
     5881 gattgccgct tcaccaaacg gggcaatcgc ctctatctcc acgtttgtag ctggccatta
     5941 cggcatattc atctggaagg atttgccggg cgagtcgact acgcgcagtt cctgcacgat
     6001 ggatcggaga tcttcttcag ggatggtgac ccaaatgcgg tcgcgcagaa caccgccatg
     6061 gcagaagatg ccggcacggt gatccttgag gtgccgttcc gccagccgga cgtcatggtg
     6121 ccggtgatcg agttgttcct cagcgactcc tagttgcagc tgcatccggc acgccgggac
     6181 aactgcgggc gctgcccccc tcacgttcac gctcatgcgt ccatgggcca ataaggagtt
     6241 cgcccgtgca aggccaccac cttggccgta tctcgcgggg cgtcatgaag cagaagtcaa
     6301 aggcgatgat tctgcgctac gtgtcttctc tccggaagat gcgactccca tcttcattga
     6361 agacttctgt ccagccgtcg cccgggtact tcgggtctga aacggcgggg aaggggccgg
     6421 gtgctaccca tcccgattgc gactgctcac tgatcccccg ggtctccgtg ggtcccacgt
     6481 agatgagtgt taccccgtag cgatcaagca catcggttga gggtggtcca ctatagagga
     6541 cgttgatatc ctcgaggcgt tgattgaccg tcgactgaaa gtccgggtcg cccaggtgcc
     6601 actgattctc gtgccctgtc cagccaatga gtgtcggtac accactgccg ctagagactc
     6661 gcccgagctg ttcccgccat tcgccgcctc cagccgcgag gagaacatct tgctcttctg
     6721 cattgtccag caaccattgg atcgcctcat actcaccggt atagagccca ttctgttctt
     6781 ccagcagata ggcaaggcca tcgacgccct tccactcgcg atcgggcgct cgccattcca
     6841 gccactgatg gccggacaca accgggtacg tcatgccgag tgcaaggatg actgtcatta
     6901 ccgcgggaag ggcgtaccgg gcaaccaagt tgcggcggaa ggctttccag atgctgacgg
     6961 cggcaagtgc tgacgcaact gccatcagga gccaaacctg atagtagatc ttgaaaatcg
     7021 tgttcatccg gctgccgccg tagtagtcag tcacataaaa gaactctggc accagcgtca
     7081 tgatcagcgc gatcgcatac atcaccaggg cgatgttcac gggcgtgacc cggaagtcgc
     7141 gctcaagcag cagccagaca acaatcactg gaagaccaca gatgacaagc agcggtgcgg
     7201 ggacgatcag cgagcccagg aagcagagga gtgctgtgac aatagcgaaa taatgaccgg
     7261 cctgatcccg ctcccatgtg acgccgcgag cctctgcgcg tgcgataagc acgtcttcac
     7321 gccgttgcca tgtttccgtg agaatcagcg tgagggcgat aaagtacatg aatccaaaga
     7381 ttgagaagta gtcctgtgga ctcgtacgct ctccctgata tgaggcgacg gacgcaagga
     7441 tgcctccgag gagcgggaca ttgcctatcc actccgaaaa gggcgtgccc gtcgatctcg
     7501 ccggcgcctc gaaggtgacg tagaaaggta tccaggcgac cagcgcggtg aacacgagaa
     7561 ggccgccacc aagaagacga tcctggacag caagaccaac ggtgccaagt gcaagggcca
     7621 gcaaggcaat gagaagatac gtagggaagt cccacgagtt cattgcgtat agtgagccga
     7681 tagcgaaacc ggcagtgacg aggcgtcctc cgtctcgcct caggagcgat tcattctcgc
     7741 gaaggcgtcc gagtgtgagg agcatccagg cgaacatcag tgccgtgact gtgtagggga
     7801 gtgccagcac atgcggatgc agatcgccga ggatgaagct gaacgacggg aactcgctga
     7861 tcggattcgc gccgctctcc gggtcgtcca cgatgatccg ggtcgattgc cacccgatgc
     7921 cgtcgaagaa ccatgcgttc cattgggact ttgcggcttc caggacccgg gacgctgccc
     7981 agggattgcc aactatgacc agaaggaggg cagcaatgaa gcccccgatg cgtgccacga
     8041 ggttgctgta ccagcgagac aggatgtttg ccgcaaggcc gaccactgcc acgaaggtca
     8101 tcgcaaaaac ggaggcgagc gccaggttga agactatggc tggactggtg ccgatcatgt
     8161 tgccgaacgc ggcccagatc gcatagccaa gatagtaata gttgaccggt tcaccggcga
     8221 gccaggcgtc cgcggggggc atcgacgttg agcgcatgct cgaggcaagc atcatcaggt
     8281 ccgatggttt ctcctgcagg tttgctgccg gtccgtagcc gcggaaccaa aggaagaagc
     8341 cgaatgccac cccgtaaccg gcttcggcga tcaggagatg tcgaaaggtg ttggcgttga
     8401 gtacaccctc acgccagccc agtccccatg cggtagcgcc gaggacaaga gctgagatcc
     8461 agagcgtgac ggcagtgaac gggacaatgg cgtcgccaat gctggcgagg aaccagaccg
     8521 gccagatgag aaagagagcg gacaggggac gaacgacgct tgcaccacga tcggtcatgc
     8581 gtcgaaagac cagtaccgtg aatggcgcga gcccgatggt caccagggtc gtgacaaggt
     8641 accagcgaag ggtgacgtcg aaccagtcca aagaggtagt cctcggcggc ccggcggaga
     8701 tcggtacgag atggctccgg accacgtgag cggcgcgcct gcgccgggat aatcgggctg
     8761 tacgcggcaa gcatcgccgg tgccaatgct acccgatcgg tgtagtggcg gcagcaggtt
     8821 cactggcgca tcttccatgc gcctgcctag gcgcggcgcg gcgtacaaca acggcctctc
     8881 gtgggcacca tatctggaag aatgcgctca acatagacgg tcaggggtat ggggttttta
     8941 gattagcccg cgccggatgg cttcggccac ggcatgcgcg cggtcgacag caccaagctt
     9001 ctggaagatc gattgtagtc gtttcttgat cgcaatctcg ctgcggttca tggcgatacc
     9061 gatttcgcga ttggtggcgc cttggctggc ccggcggaga atctcccggt catcctccga
     9121 gaggtcggca tctcgtgcag tcttttctcg cgcgagcccg ccgaactgct ccagaactcc
     9181 attcaatagc tcagggctga gctggcgccg accgcgggac gcatcgcgga tggaactgat
     9241 gagctgagcg gagggagtgc ttttcagcag gtacccatcg gcaccggctt ccagtgcctg
     9301 ggtgacgtac gcctggtcac cataggacgt caggacaata atggcgccct catactcctg
     9361 ctcacggaga cgccgggctg tctggatgcc cgtcgcgcct ggcatgcgga gatcaagcag
     9421 gatcacatcc ggctgcagga gtttggcctg cgtgattgcc gacgatgcgt cgtgcgcttc
     9481 gccggtaaca tcaatatctc cggccacgtc tagcaattta cggaggcctt caatgacgac
     9541 aggatgatca tcgaccagaa gtacggagat cacgtacggg gtatcctatg aatcacgacg
     9601 cccctcatca tctgtttccg atgtgccgga agcgtcctcg ttccctgtat tgtccgatga
     9661 atgaactaca tcgaccacct ggctttcgtc gtcccggtgt cggatcaggt cgtccaggat
     9721 ttcatccgac atgacgtttc cggtttctgt tctctcaatc acaatcgcgg tgccgccccc
     9781 gggtttggaa atgatggaga acgtggcgcc gatagcggcg gcacgttccc gcatgctcag
     9841 catccccagg gaggtgggtc ggactgccga tcccggtgtg aaaccggccc cgttatcacg
     9901 gatggtgacg cgaagtaaat cactcgcgaa ctcaatgcgc acgacggcct cgtcagcact
     9961 ggcatggcga accacgttgt ggagtgcttc ctggacgatg cgataaattc cgagccgaac
    10021 ttccggcgtg aggggtggca gatcgttgat gacttcaagc cgcgcctgga gtcccgtccg
    10081 ctgaatcgtg gagacatatg cttcgagcgc tgctacaacc ccgatgtcgt caagatctgg
    10141 tggtcgaaga tcgaagataa ccccgtggag ctcttcagac gcgcgttcga gcaggctgtt
    10201 tacactctgg agcgtctcat tcagggtctc gtgagtacta ctgccattcg ccatctgcag
    10261 cgcacgtgcc tggtaaaggg ccccggtgat cagtggcgaa acagcgtcat ggatctcgcc
    10321 agctatgcgc cggcgctcgt cctcctgaag ccgaagcgtc cgtcgtagca gctctcgcat
    10381 ctggtcggtc tggcgctggc gctcgaggtg actctgttcc aggccggcca cctgcccacc
    10441 aagacggttg ccgagctcag agagtgcggt actcaagctg agtatctctc caccgccgtt
    10501 tggctggata gagacactgt aatcgccttg ctgcaacaac tgcgagtccc gtgccagatc
    10561 acgaagaggc cgggcggtgt actcaccgat cgccacggca atagccagaa cggcaatcgt
    10621 tgccaaggcg agcataagcg cgccctccag ccagagagaa tccccatagg gccttggaac
    10681 cgggaacgag acaataaccg cccaatcggc gaactcaagc gggactggcg cgaaggtagc
    10741 aaggcgctcc attcccgagg tactggtcat ctggaagacg tcggtgccgc tggatccggc
    10801 acggatgtcc tcttcatcga tccggcgaag gaaatcttcc tcgttttgcc tgatgtcccc
    10861 tgttgcggca atcacctcgt cacttgagac gatcgcaata tctgcgcggg tgcgaggaac
    10921 ggggatcacc gactgggtca tggtgtcgat ctggacgacg gcgcccacta ccccgaggat
    10981 cgtacctggc ggttgttgtc cgggcgtcgc ttccggcgtg acggtggttg tggcttgtcg
    11041 ctcacgaccg ttgtcgtcgg atggcggagt tggagttggt gcatctcctg gtgcggcggc
    11101 ggttgtctgg gtctgcgtga tcgtcgtcac cggtgcgagc agaacgataa cgttggtttc
    11161 atcggcgagt tcgatccgcc cggagatcgt gcgttgcccg gtggtgaggg tggtttcgat
    11221 ctgattcctg atgtcaggaa gtaccagttc agcatcggtt ccggattcgc cgacgacttg
    11281 ttcgcccgtg ttgatgagga agattccgct catctcaggc ctgagtgctc gcgcctcccc
    11341 cagcacgtgg ttgaccgcat cgatgttgtc ctgttgctgt atgacgggca cgcctgagag
    11401 acgctcaatc gtctggtatc tcgagttaat gtaattggcg agggactggg tcacgagcgt
    11461 tgagatcgtg gcttcgccgg tgatgatttc ctgattgctt tgctccgcac tacgatcgag
    11521 aaagaagatg atcacgatga ggagcgggat caggctgatg agcgcgaaca ggatgaaccg
    11581 tgtccggacg gaagtatgcc ggaacatcta ggtggctgcc ttacagaaca aggggagggt
    11641 ggcggcgcgg cgcgggcgca gcacaacgcg gaggcaatac gcagcgagcg aagggcaccc
    11701 cgacgaagag ttggacggca acgcaacaag acgcgccggt gttacaggcg aggagctcga
    11761 catatcgcgt taataatcgg cgatgtcgga gcaagaatcg atatacacaa gaacccccag
    11821 cgctatccat tagcaccccg cgagtgtccg tgggccacgt gtcggatgta cagctactcc
    11881 acggattcat gtttcgacga gggcattgga ctgcatttgt cggtgcatca taccccattg
    11941 gcaaggatcg actgttgcct ctcgagagta gttgcgacat ggtacatgaa tcacggcatc
    12001 gagtccgagt tgtgatcgcg cgtaccggga gagaacggac atgagcaagg tcgttgtggt
    12061 gggagggggg attgtcggtg caagtgccgc gtttcatctc gcccgtgaag gggtggaaac
    12121 ggtacttgtc gaccggcggg atgcgggccg ggcaacctcc gccggggccg gtattgtcgc
    12181 gccgggatcc agcctcaggg attctccagg cttctatcac cttgcactgc cggcggttgc
    12241 ccattacccc gagctagttg cggcactagg cgaggtagat gcaggcgaga cgggctacca
    12301 ggtgtgcggc aaaatcttcc tggcagagac tgacgaacag gcctgggatc tggacaatgt
    12361 cctggcgctc ttcaacgagc gacgggcgaa cgggatgccc aatctcggtg agatccagga
    12421 tatcgacgag agggaggcgc agcgcctatt tccggccctg ggagtggttc cgcgagcgct
    12481 ttatattcct gaggcggcac gcgtagacgg agcgctgatg cgcgacgcgc tcacgacggg
    12541 agcgcgacac tatggtgctc aggtaatgct tggagctgcc aagccggtga tggatcaggg
    12601 gcgtgtgatc ggcatcagct gcgatggact gttgatcgag gccgacgccg ttattcttgc
    12661 cgcgggcgct tggtcaaagg agatgttcga gcctctggga ttttcgctgc cggtggctcc
    12721 acagaaagga cagatcctgc atattcgact cgagggccag gagacaacgg actggccgat
    12781 actttcctgg ttcggtgatc agtacatttt ggcgttcggg ccggatcgtg ttgtcgcagg
    12841 ggcgacgcgg gagtttggtt ccggcttcga cacccggatt actccggggg gtgtaaagca
    12901 cgtcctggat acggccctga gaatcgctcc ggggctggcc aatgggacac ttgccgaggt
    12961 gagggtcggt ctccggccct atgccgaaga cagtttgccc ttcatgggca tggcacccgg
    13021 acacggcaat gttgtcgtag ctacggggca tggcccctcg ggcttgcaac ttggaccgta
    13081 ttccggctac ctggccgctg aactggtgat gggccgaacg cctcccattg acctgactcc
    13141 attcgccgtc gatcgcgaaa ttctcgcatc acaggggtaa tgtaccaatg actacaccac
    13201 aacagggaat tacgtttggc attgtcactg ggcagcatca gctgacatgg gaccagatcc
    13261 gcgacatgtg gctgctcggt gaatcattgg ggttcgagtc tctctatctc ttcgatcatt
    13321 tcaccgggct ttacggagac ccagccgggc catgccttga ggcctcgaca ctgctcgcag
    13381 ccttggcact cgagacggag cacgctgaca tcggtgtgct cgtgtatgga aatactcaca
    13441 ggcatccgtt cgtgctggcc aaggagatcg tgacagtcga ccatctcagc aagggccgag
    13501 cggtgcttgg tattggcacc gggtggaacg aaaatgagca ccgttggtat ggcattccgc
    13561 tccattcgcc ggggacgcgc gtggcgatgc tcgacgaggc gctgacggtg atcgacgggc
    13621 tcctcgccaa cgagcgtttc accttcgccg gacagcatta ccagctggac gatgccccct
    13681 tcgctcccaa accggtccgg gagaagatgc ctatcctcgt tggcgggaag aagccgaaga
    13741 tgcttcgggt cgttgcaagg cacgcagata tctgggacgc cagcggcacg ccggaagttc
    13801 gcaaggagcg aagtgaggaa ctgcggcgcg cgtgcgacga ggtcgggcga gactaccacg
    13861 aaatccggca ctccatgtca ttcggtgcgg acaagctggt cgacatcgat cactttgaag
    13921 agctggtgac ggcctataag ggagtgggcg tccagcaatt cctgttcgat ttccctctgg
    13981 acaaggatgg gcagcagatc gcgcggaagg tggcgacgga gctgatgccg aaactgcggc
    14041 tggcgtagac tcgtgctccc gccatccgag gcgtgcaaca actcaagcac gatgaaaggc
    14101 agttagggtg cagatctccg agatgatcca caggcggatc gaactggtga cgttcgatct
    14161 ctacgacacg cttgtcgagg cgaacccgcc acgttgggag cgattcgcgg tggcgctgga
    14221 acgctccggc atgccgatcg atgtggaagc cgtgctgcat gccgatcgca agggtgagga
    14281 tcattacacc attgagaatg gccgctaccc gatcagggat cgtacggaca aggaagtgca
    14341 gcagtttcgc ctggatcagg cagcagccta cctgcatgcg ctggacctgc caaccgatcc
    14401 agaaaccgtg agaaccgtgc aggaacattt tcgggatgag actcaaaacc atggtggctg
    14461 gtcgtaccgg gcgttcgagg agatcccgaa ggtgatggaa tgcctggagc agcacggcat
    14521 caagcgagcc atcatctcaa acgccgatgc ggatgtcacc cggttctgcc tgcagatggg
    14581 gtttgccgcg cacatggacg tcatcgtgac ctcggcactg gttggatggg agaagccgga
//
LOCUS       DASYWM010000149         7177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_362064, whole genome shotgun sequence.
ACCESSION   DASYWM010000149 DASYWM010000000
VERSION     DASYWM010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362064"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..708)
                     /locus_tag="VGR29_06095"
     CDS             complement(226..708)
                     /locus_tag="VGR29_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEV2073198.1"
                     /translation="MKVYLGGPMFVAAEVRYNLWLAEQLRSRGHEVYCPNESEPINDK
                     TRTDITARAIYDADIEALEACNVYICQVSEDSGTNWEAGYMDCLSRHVDPERYLGVIG
                     LATDIRLQQMPVPERSGIENQAFHVNSFIVGGMQASLGVVYDEESLFSRLGDIAAHQD
                     "
     gene            complement(705..1211)
                     /locus_tag="VGR29_06100"
     CDS             complement(705..1211)
                     /locus_tag="VGR29_06100"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV2073199.1"
                     /translation="MNTVGEPVPINVFSDHHCFGCGRLNIHGLHLMFYENVDGNGVWA
                     PFTPTAAFEGYGGIVHGGIVCTILDEVMAWSLYREQTWAVTGQLSTRFRKPLVMGEPV
                     VAIGKIVRDRGRVIDMRGEICRASDDVVLAEGTATFFRVPEAQAAEWNERYQSGPVTL
                     TARDEGEM"
     gene            complement(1225..2049)
                     /locus_tag="VGR29_06105"
     CDS             complement(1225..2049)
                     /locus_tag="VGR29_06105"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEV2073200.1"
                     /translation="MHEERHDEFVEELPAPAVLDSMEEMIRISRRMVSFTGAGISTES
                     GIPDYRGPNGVWQTGKIPHIDTVRTDEEARKESWQRRLETYPTMLARVPNAGHDALAR
                     FEKAGKLLAVITQNIDGLHQKAGNLPERVIELHGSSHRLHCLNCGATYDGVTIQARLE
                     AGEPDPRCDVCGGPLRTSTILFGESLPVEALQLAHRVSMAADLMLVVGSSLVVNPAAR
                     LPQIARQQGAGLIIINRTPTPLDGLADIRVAAEAGPTLSELARRVLGPVSACGTGK"
     gene            2604..3044
                     /locus_tag="VGR29_06110"
     CDS             2604..3044
                     /locus_tag="VGR29_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073201.1"
                     /translation="MEPTQRTVPAGEETISHTVSTFQWRRHVQLLDRPTSVLPEEERR
                     ARQHAVQGLYAARDGALEIAEHHFTSAAASPSIFLCEIPGFWQLSRSAMMTAVHAYDK
                     AGRLRDASALNARIRTQYRPQAVSPLPANVTELPTRRLGLTGNS"
     gene            complement(3776..4912)
                     /locus_tag="VGR29_06115"
     CDS             complement(3776..4912)
                     /locus_tag="VGR29_06115"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2073202.1"
                     /translation="MGELVVGRPALQVKTALSIPLDMVSIMSLLYRAVPGSGLDPWLV
                     LTRRALPRQLQDDLDLLHGFSGRLLYYMEEPVMAFEPLREDRLEASIDDLVAFLDAQE
                     PEAFQEYATKAIDRAHRDLGSELATPVDNDEVEWRRYVEPVLTTADATEVLDLLLQPE
                     YLKGRTIRLIQTLWDSFYCDEYRVREDSLREAERVARSVASRGFGLAFADLTGNRLPA
                     TLAAGLNTVTHVTFCPSAHLGEFVSYITYPPDLVVFFSAQHVTGTVVDHDLSALDVDT
                     SQEDRYLGSDVNPLSGEDLLESIRAMGDANRLRILDLLGTGELYAQEIVGRLGIAQSA
                     VSRHLSQLERAGLVRVEPRRGMKYYAISRDRLQMLAESILDRAR"
     gene            5161..6273
                     /locus_tag="VGR29_06120"
     CDS             5161..6273
                     /locus_tag="VGR29_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV2073203.1"
                     /translation="MADDTPSTNKRSPSHSTPPRSRFRGRDGVTSEERERPPSDLPGM
                     VEAEKRIHQARPDQLPLFDHLPESPYDSTVPTNLSELTAGSSLPLARSWYRHVLERLG
                     RPRNTVESYSYDLTVLENLIGPKPLNRITNGDIAKYLGEASSKTTRKRRLTSARRFFR
                     YLIEEEKVLKFDPTEGYYPHSIQLRLPVPLFKDEQETLMEAARMDEPWSAPAIWLMMR
                     LGLTRSELLALRRDHIDRTDPREPIVYIFYEDASKQSKERKLAANSEFSELFDAFLEE
                     RNPSDILFPVGRQAVNGMVDRVRRAAGITKDVTPNTLRHTYAVEAARGGADVQQLLTL
                     LGLADDPRNRSSVARYIRLAEPALGSEKIAEMEADT"
     gene            6494..7018
                     /locus_tag="VGR29_06125"
     CDS             6494..7018
                     /locus_tag="VGR29_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073204.1"
                     /translation="MTDGQMFVVALRVVLPLLILRKPLLGGILAMLLDALDVVIVELF
                     GAGGMGHHYHSIDKVLDLYYLGLEAWVALSWIERIPRISAIALFLYRVAGVVIFELTQ
                     WRPTLFIFPNLFEHWFLFYLIRSRFFPGLRLNAWKNTIIWLAILYIPKLAQEYLLHVA
                     QAQPWDWVKGVLGI"
     gene            complement(7015..7176)
                     /locus_tag="VGR29_06130"
     CDS             complement(7015..7176)
                     /locus_tag="VGR29_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV2073205.1"
                     /translation="MGDDADTTATVAGALTGAAYGVEAIPQRWRSVLHGRLEIEALAR
                     QLLAWDMQG"
BASE COUNT         1522 a         2258 c         1963 g         1434 t
ORIGIN      
        1 tcgacgattc gagcattggg gatcttctgc gcagcctcaa tctgcttgtg gcaaccgcgc
       61 agcaccacac cgcggaggca ctggcgggcg aaggtgagac gccagacgac gaggcacaat
      121 ccaccacgga cgctgccacc ggtacctggc ccagggacga acagtaacgc tgacatggcc
      181 gcagccgtgg gggttcaggc tgcaaccatc agctgccaat ctggttcagt cctggtgtgc
      241 tgcgatgtcc ccgagtcgcg agaagagcga ctcctcgtca tagaccaccc cgagcgatgc
      301 ctgcatacct cccacgatga atgagttaac gtggaaagcc tggttctcga ttcctgagcg
      361 ctctggtacc ggcatttgct gcagccggat atctgtcgcc aacccaatca ccccgagata
      421 ccgctcaggg tcaacatggc ggctcaggca gtccatgtag ccggcctccc agttggtgcc
      481 ggaatcctca ctcacctggc agatgtatac gttacacgcc tcaagcgcct cgatatccgc
      541 atcgtaaatt gcgcgggctg tgatatcggt ccgcgtcttg tcgttgattg gctcactttc
      601 gttcgggcag tacacctcat ggccacgcga ccgcaactgc tccgccagcc acaagttgta
      661 ccggacctct gcggcaacaa acatcggccc gccgagatag accttcacat ctccccctcg
      721 tcacgtgctg taagcgtcac tggcccgctc tggtatcgct cgttccattc cgccgcctgc
      781 gcctctggca cgcggaaaaa ggtagccgtg ccttcggcaa ggacaacatc atcggacgcc
      841 cggcaaatct cgccgcgcat atcaatcacc cggccgcgat cccgtacgat cttcccgata
      901 gcaacgaccg gctcacccat caccagcggc ttgcggaatc gtgtcgagag ctgaccagtc
      961 accgcccatg tctgctccct atagagcgac catgccataa cttcatcgag gatcgtgcag
     1021 acaatcccac catggacgat gccgccgtaa ccctcaaacg ccgcagtggg cgtgaacggc
     1081 gcccatacgc cgttgccatc gacattctca tagaacatca ggtgtagccc gtgaatattg
     1141 agccgaccgc atccgaaaca gtgatgatcg ctgaacacat tgatcggtac cggttcacct
     1201 accgtattca tacgcgggcg atcctcattt ccctgtcccg cacgcagaca ctgggccaag
     1261 aacccggcgt gcaagctcgc tcagggttgg acctgcctcg gccgccacgc ggatatcggc
     1321 cagtccatcg agtggcgtcg gcgtgcgatt gatgataatc agaccggctc cctgctgtct
     1381 agcaatctgc ggcaggcgcg ctgccgggtt cactaccagg gagctcccga ccaccagcat
     1441 gagatcagcc gccatcgata ctcgatgcgc gagctgaagt gcctctaccg gcagcgattc
     1501 accaaacagg atcgtggagg tacgcagggg tccaccacag acatcgcacc gtggatcggg
     1561 ttcaccggct tcaagccgag cctggattgt gactccgtca tatgtcgcgc cacagttcag
     1621 gcagtgcaag cgatggctgg acccgtgcag ctcgatcacg cgctccggca gatttccagc
     1681 tttttgatgc aacccgtcga tattttgggt gatcacggcc aacaacttgc cggccttctc
     1741 gaaccgtgcc agtgcatcat ggcccgcatt gggcacccgg gccagcatcg tcggataggt
     1801 ctccagacgc ctctgccagg actccttgcg cgcctcctcg tcggtccgca ccgtgtcgat
     1861 gtgcggaatt ttccccgtct gccagacacc gttgggacca cgatagtcag gtatgcccga
     1921 ttcggtgctg atgccggcac cagtgaacga gaccatccgt ctactgatcc gaatcatttc
     1981 ctccatgctg tcgagcaccg ctggcgcggg aagctcctca acgaattcat catgtcgttc
     2041 ttcgtgcata cgatatgtcg tctttcagct cggtggatcg agagtagtgt accgccagtt
     2101 ggggtcggta aaggtctaca ggaatgtgga cggtgcttac accccgaagc accaggaatt
     2161 ttgcccagct gtcagcaaaa taaggccctg atcccttgtg tacgtacggc tctcgtggta
     2221 ggatcacttt atccgattct gtgactagcc gtcacagcgc gtagagcccg tgccgttcaa
     2281 tggccccgca accatatgga cattttccct atagaagcat gctgtcatcc ggcctcctcg
     2341 cggagcccgg agggcgagcg tagcggcggt gcggtctgga gaatgccatt cattcccgca
     2401 ctccacccgg acgatcagtg cgaaagaagg ccgtcatgat catgactcaa cttgcgatga
     2461 tggatgcagg accagtgggg aactccatag ttgcacgggg gttacgcgcc atctctcaat
     2521 ttttcggatt tggccagcta cccacagggc agctgaggtg cttttcctcg acaatcccgc
     2581 tggctgatcg ccagggagtc tttatggaac cgacacaacg aaccgtcccg gcaggcgaag
     2641 aaacgatctc ccatacggtc agcaccttcc agtggagacg ccatgtccag ctccttgacc
     2701 ggccaacgag cgtgctcccc gaggaagagc gcagggcgcg tcaacatgcg gtgcaggggc
     2761 tctatgctgc gcgcgacggg gctctcgaga tcgccgagca ccactttacg tcagccgcag
     2821 cctctccctc tatctttctt tgtgagattc caggtttctg gcaactgagc cgatcagcaa
     2881 tgatgactgc cgtccatgcc tacgacaagg ccggacgcct gcgggacgcg agcgcgctta
     2941 acgcacgcat ccgtacccag tatcggcccc aggccgtctc tcctctgccg gcaaatgtga
     3001 ccgagcttcc aacgcgaagg ctcggcctga ccggcaactc atgacgtgct gatccgggga
     3061 tggagcgtcg tacgcacccg gacccaaaga ttccgattct tttgatttca ctgacaaggg
     3121 atgacggcga aaccatccac acacaagtga tcgggtaacg gtcagggccg acgatccgga
     3181 ggtctgatgg tgggcatcga tatcgcattt ctgcgatatc gctgcccacc acttttcggt
     3241 gaagatgctg tacaatccac tcggcggata aacctgtagt cggctaatcg acgacggtta
     3301 aaaaaaattg cgcccggtgc gtgtgcaccg ggcggcaact tctgccgacc gcagccgagg
     3361 gataccagcc tgatcgcggt cgagggtcag gcgtcggaga ggaaatgtat gaagtggtac
     3421 cgatatcatt cgtttccttc agatatcagc acactaaaga aggaagaaga ggtttaatat
     3481 gccaggcgac cggctaacgt cgcccgaggc ggctaaggaa tctacggccc gcgatggttc
     3541 taatagatcc tggaactggg tggacatcgc gatccggttc ggccctgcgt catccatcgc
     3601 tctcacgatg gcggcgagcc attgggacaa tattccattg tcaatgtgcg tccgcttgcg
     3661 cggctcctag atcgtgtctt cctgatctgt cagtatcata tcatcgcgat attgcgttga
     3721 caagcccctg gaccagtctt gtaacgagat tcacaatcta gataacccta cgcaactatc
     3781 gtgccctgtc taaaatggac tccgccagca tctgcaaccg gtcgcggctg atggcgtaat
     3841 acttcatgcc ccgccgtggt tctacgcgga ccaatcctgc ccgctccagc tgcgagaggt
     3901 gtcgcgacac cgccgactga gcgattccga gccgccccac gatctcctgc gcgtacaact
     3961 cgccagtccc gagcaaatca aggatgcgca accgattcgc gtcccccatc gcccggatgc
     4021 tctcaagcag gtcctcacct gagaggggat tgacatcgct ccccaggtag cgatcttcct
     4081 ggctggtgtc gacatcaagc gcagagaggt cgtggtccac gaccgttccg gtcacatgct
     4141 gtgccgaaaa gaacaccacc agatcgggcg gatacgtgat gtaactaacg aactccccca
     4201 aatgagccga cggacaaaac gttacatgag taacggtgtt gaggccggct gcaagtgtcg
     4261 ctggcagccg gttcccagtg agatcagcga acgcaagtcc aaagccacgg ctggcaaccg
     4321 accgcgcaac cctctccgcc tctctcagcg agtcttccct tacccgatat tcatcgcagt
     4381 agaaggaatc ccaaagcgtc tggatcagac gaatggtccg tcccttcaag tactccggct
     4441 gcagcagcag atcgaggacc tcagttgcat cggctgtggt caggaccggc tcgacatacc
     4501 ggcgccactc gacctcgtcg ttatcaacag gtgtcgccaa ctctgatccc agatcgcgat
     4561 gcgcccgatc gatcgctttg gtggcgtact cctgaaatgc ctccggttcc tgtgcgtcaa
     4621 ggaatgcaac caggtcgtcg attgacgcct caaggcgatc ctcccgcaag ggctcgaatg
     4681 ccatcaccgg ctcttccatg taatagagca atcgcccgga aaaaccatgg aggagatcca
     4741 gatcatcctg caactgacgc ggcaacgccc tgcgcgtcaa aacgagccac ggatcaagcc
     4801 cactgccagg aacagcacgg tacagcaacg acatgatgga gaccatgtcc agaggaattg
     4861 aaagtgccgt ctttacctga agcgccggtc gccctactac gagctcgccc atgcattact
     4921 ccgtcaatag ggatggcgca gtgccgcccc ggagggattg aaaacaggtt gcggtatatt
     4981 tagggcatca ttgccggagc atggaatccc agctgctcac ggttttataa tgtcatatca
     5041 ccatattagc ataatccagc cgtcaatcgg tacaacgttc ccagtcgcga acttcgccag
     5101 cgaaccgaca ttccaacccc ctaaaccaca tagcagcacc agtcgcttcc ggaggagatc
     5161 atggctgacg atacaccctc caccaacaag cgctctcctt cccattccac ccctccccga
     5221 tcccgcttcc gggggcggga tggcgtcacc tccgaggagc gtgaacgtcc accaagcgat
     5281 ctccccggta tggttgaagc cgagaagagg attcatcagg cgcggccgga tcagcttccg
     5341 ctcttcgacc acctgccgga gtccccatac gattcaacgg tcccgacaaa cctctcggag
     5401 ctgacggcgg gctccagcct tccgttggcc cgcagctggt accgtcatgt gctggaacga
     5461 ttaggacgcc cgaggaacac agtcgagtcc tacagctacg atctgacggt gctggaaaac
     5521 ctgattgggc caaagccatt gaacaggatc accaacgggg atattgcaaa atatctcggc
     5581 gaagcctcca gcaagaccac ccgaaaacgt cgcctgacct ccgcccggcg cttcttccgt
     5641 tatctgatcg aagaggaaaa ggtgctgaaa tttgacccga cagagggcta ctatccccac
     5701 tccattcaac ttcgcctgcc ggttccactc ttcaaagacg agcaggaaac gctgatggag
     5761 gcggcccgta tggacgagcc gtggtcggct cctgcgatct ggctgatgat gcgtctgggg
     5821 cttacccgct ccgagttgct tgcgctgagg cgggatcata tcgaccgcac cgatccccgt
     5881 gagcctatcg tctacatctt ctacgaagac gcttcaaagc agtcgaagga gcgcaagctg
     5941 gcggccaact cggagttctc cgagttattc gacgccttcc ttgaggaaag gaacccgtca
     6001 gacatcctct ttccggtcgg ccgtcaggct gtgaatggga tggtggatcg ggtccgccgg
     6061 gccgccggta tcaccaagga cgtgaccccc aacaccctgc ggcataccta cgccgtggaa
     6121 gcggcacgcg gcggcgccga tgtgcagcaa ctcctgacac tgctgggact tgccgacgat
     6181 ccccggaacc gttcctcggt cgctcgttac atccgcctcg ccgaacccgc cctcggcagc
     6241 gagaaaatag cggaaatgga ggccgacaca tagcccgatg ggagcaacca ggttagccag
     6301 ggcggatcgt gatttccagg gaacgatata gcgggacgcc acatgagctg gactatcggc
     6361 gcgcaggtaa cgctgatcac gcaacaatcc tgaaggacat tctcttcgcg atcagtggtc
     6421 gttgccttgc ttcaagagga cagattggaa ccctgaagca cttcttgcgg gaacctggaa
     6481 cgggagtgtc atcatgacag acgggcagat gttcgtggtg gcccttcgcg tcgtcctccc
     6541 gttgctgatt ctgcgcaaac ccctgctcgg gggcatcttg gccatgctgc tggacgcgct
     6601 cgatgtggtc atcgtcgagc tcttcggagc gggagggatg ggtcatcact accacagtat
     6661 cgacaaggtg ctggacctct actacctggg gcttgaggcg tgggtggcac tctcctggat
     6721 tgaacggatc ccgcgcatca gcgccattgc tctcttcctc tatcgcgtgg cgggcgtggt
     6781 gatcttcgag ctaacccaat ggcgcccaac gctctttatc ttcccgaacc tctttgagca
     6841 ctggttcctc ttctatctga tccgtagccg cttcttcccg ggtctgagac tgaacgcgtg
     6901 gaagaatacg atcatctggc tcgccattct ctacatcccc aaactcgcac aggagtatct
     6961 actccacgtc gcccaggcgc aaccatggga ttgggtaaag ggagtgttgg gaatctagcc
     7021 ctgcatatcc caggccagaa gctgacgagc cagggcctcg atctcaagcc ggccatgcaa
     7081 gacgctccgc caccgttgcg gaatcgcctc aacgccatag gccgcgcctg tcagtgcgcc
     7141 cgcgaccgtg gcggtggtgt cagcgtcgtc ccccatc
//
LOCUS       DASYWM010000150         7499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_531337, whole genome shotgun sequence.
ACCESSION   DASYWM010000150 DASYWM010000000
VERSION     DASYWM010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7499
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531337"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="VGR29_06135"
     CDS             <1..1565
                     /locus_tag="VGR29_06135"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV2073206.1"
                     /translation="SMIEEVSAGELRTGLDSGTFTVRELAQACLDRIEAMDRTGPALG
                     AVIQINPDALTIADELDAELQDGRSRGPLHGIPVLLKDNIATTDRMQTTAGSLALEGA
                     VPRKEAFIAERLRAAGAVILGKTNLSEWANIRSSSSSSGWSARGGLTVNPYQLDRTAS
                     GSSSGSAVAVAASYTPLAIGTETNGSISSPSGSCGIVGIKPTIGLVSRAGIIPISHFQ
                     DTAGPMARNVPDAAMLLNAIVGDDPNDPAQHGPRLESEPSYPARAAESLPHVDYTSVL
                     DPEGLRGARIGVLRSPLAQSEATQEVFGQAIDALREAGADLVDPLTFPSEQKMTKNRD
                     ILSVMLWDLKTDLGAYLRDYVDPEFPIRRLADVVDFNREHAETEMPWFGQDLFEMALE
                     KGSLDDPAYLSMVAGVQRLGRTEGIDALLKEHRLDALVAPTNAPASKIDLLNGEHHVG
                     GSSTPSAIAGYPIVTVPAGYAAGLPIGLSFFGTAYSEPTLIRLAYSFEQATQVRHAPA
                     YTAPGVLPPEHQ"
     gene            1748..2863
                     /locus_tag="VGR29_06140"
     CDS             1748..2863
                     /locus_tag="VGR29_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073207.1"
                     /translation="MQDRPPPTDPDERRPSSDSGVTPDDVGDAPELDQPDERLSPDVD
                     AEPRQVRVTPTARRRNAPQPGPAQDERDRAEPVEAPAAYTPGLEGQPQATAARSRPWT
                     WPLISLIGLLAVVFVNWLANWLPLNDQTTGEISRENPVPFQPAGWAFLIWPLIYAALF
                     AFVIYGFLPAGRRNSRVRAIGPVFLVANIANIAWIFLWHWERFAASFVAIVVLLGSLA
                     VIYAMLPGPRGNLSSVSRVQRLLVRVPFSLYLGWISIATIAALEVWMINGGWNGGPFG
                     LRGWTVVFLIAGVLVAAVFAFAAKDAIYPLVFAWGYFAIAYEQWDGSSLISILAGSLA
                     IVAAVLTVMAVMLSFGARAGSALPAMPRQVDKPPTAP"
     gene            2920..4038
                     /locus_tag="VGR29_06145"
     CDS             2920..4038
                     /locus_tag="VGR29_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEV2073208.1"
                     /translation="MPRELVAVAPRTPQLREYEERPPGPHDVRVRTEIASPKHGTELV
                     AYRNDPVASRPYDSLWGAVVPSAADDALARFPMRLGNMAVGVVTDIGAEVSRFTVGDR
                     VFGHFPIRETQTVDEMQTDLLPPGLSAEAALCLDPVVMALAMRDGGIKLGDRVAVFGL
                     GAIGLIAIQLARLAGAAQVIALDPLENRRNLALALGADIVLDPTAHGGDAGLEIRKLT
                     GGDAPEPEIPTSARIIGGYSEAPTQLTELGVDVAVETSGSIPALQQAIRATRFGGTVC
                     VVSFYGREAAGLLLGEEFHINRLQLVSVRSQSLPMRDAPAWTLERVVALGLDWLVEGR
                     IRTEGIITPILPFEESVDAYRMIDERPAESIKLGIRFS"
     gene            complement(4329..5120)
                     /locus_tag="VGR29_06150"
     CDS             complement(4329..5120)
                     /locus_tag="VGR29_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV2073209.1"
                     /translation="MKLTIREAMVPGETFADQLAWLETMGIDGIELHAGSLELPREEL
                     HDAFAGSTVLASAIEGIPRLLDADPAVREDAFRRTRERLTLAGELGAVGVLVVPQFGR
                     TAALHDLSPWKTGAELERELLLCQLGELAPTAREAGVHLFLEPLNRYEAYIVNRVEQG
                     AEIAREAGGAPVATMADFFHMNIEEADIAASIHESANHIVYVHVADSNRLQPGQGHLD
                     FQPGFAALKDIGYDGWLGIECRVSGDYDTSMRTTAAHIRKEWEEA"
     gene            5351..6682
                     /locus_tag="VGR29_06155"
     CDS             5351..6682
                     /locus_tag="VGR29_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2073210.1"
                     /translation="MKQRRFTGFWTIAACFLLVLTSIGLQPAAAQETVELTVWDTHTG
                     EVENAAADQIYGAFMEANPNIEITREVYSVDNMRQTVNTAIASGTGPDIIFYDAGPGY
                     AGVLAQAGLLTPLDEYAEQYGWGERIAEQSLEATTLGGELYGLPLQVDLIGMYYNQTL
                     IEEAGLTVPQTYEELLTFCQGASEAGYVPLAFTNNPGWQGFHQFSMIVNNMLGPDWVQ
                     EKLLNNQGEWDSPEVVEAINSYFVEMRDAGCFSSDVNALTNDDANALFQSGEALMWPT
                     GSWMASGLNSETMPDMELGYMPFPALEEGAGSFWVSGVGSAYYITETSEHKDEAALFL
                     DYLFSPTVAAQWVDEAGFFVPMAVDKDTLELTPLQDAILTELQRAAAGEIELGYNIDV
                     LAPPRFNETLQSGIQAVLAGDMTAEELATRIQAAWEESWQGQGTPEASPAS"
     gene            6775..>7499
                     /locus_tag="VGR29_06160"
     CDS             6775..>7499
                     /locus_tag="VGR29_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2073211.1"
                     /translation="MAVMEAAPSDTRSHRLRRSWTEYRAGILFLLPGFVLYAIFMIYP
                     FFQSIYLSFTDWNGAEPVKNFIGLENYRNLLTDNLLWKSLRNNVIWVVIGTLGPLALG
                     FLLAVLLASRPKGFTLFRTAYFMPQVLSPVVIGIVWGWMYNPLFGIFNVALERVGLES
                     WSRGWLGDPDWALYAVLLAAMWAETGFVFVVFLAGLQNVSRDLIEAATLDGANAWRRF
                     WDVTVPQMANVVNVVTALLLIGG"
BASE COUNT         1308 a         2268 c         2446 g         1477 t
ORIGIN      
        1 agagcatgat cgaagaggtt tccgccggag agctgcgaac ggggctggac agcggcacgt
       61 tcacggtgcg ggagctggcg caggcgtgtc tggatcgtat agaggcgatg gatcggactg
      121 gtccggcgct gggtgcggtg atccagatca acccggatgc ccttacgatc gccgatgagc
      181 tggatgccga gctccaggat ggccgatcgc gtgggccgct ccacggtatc cccgtgctgc
      241 tgaaggacaa cattgccacg acagaccgga tgcagacgac agccggatca cttgccctgg
      301 agggtgccgt tccccggaag gaagccttca tcgccgagcg cctgcgagcg gcgggcgcgg
      361 tcatcctcgg caagacgaac ctctccgaat gggccaacat tcgctcgagt tcctcctcca
      421 gtggctggag tgcccgcggc ggcctgaccg tcaatcccta ccagctcgac cgcacagcat
      481 ccggctccag ctctgggtcc gcggtggccg tcgcggcgag ctacacgccg ctcgcaatcg
      541 gcacggaaac caacggatcg atctcctctc cctcgggaag ctgcggcatc gttggtatca
      601 agcccacgat cgggctggtg agccgggccg gaatcatccc gatctcgcac ttccaggaca
      661 cggccggccc catggcgcgg aatgtcccag atgcagcgat gctgctcaac gcgattgtcg
      721 gggatgaccc gaacgatccc gcccagcatg ggccccggct tgagtctgag ccctcatacc
      781 cggcccgggc cgcggagagc ctgccgcatg tcgattacac aagcgtgctg gatccggagg
      841 gtcttcgtgg agcgcgaatc ggggtactcc gttcgccact cgcccagagc gaggcaaccc
      901 aggaggtctt cgggcaggcg atcgatgccc tgcgcgaggc gggggccgat ttggtcgatc
      961 ccctcacctt tccctccgag cagaagatga ccaagaaccg ggatatcctc agcgtcatgc
     1021 tctgggacct gaagacggat ctcggcgcct acctgaggga ctacgttgac ccggagttcc
     1081 cgatccggcg actcgcggat gtggtggact tcaaccgcga gcacgccgag accgagatgc
     1141 catggttcgg gcaggatctt ttcgagatgg cgttggagaa ggggtcgctc gatgatccgg
     1201 cctacctgtc gatggtggca ggggtgcagc gcctggggcg cactgaaggc atcgatgcgc
     1261 tgttgaagga gcatcggctg gatgcgctcg tggccccgac gaacgcacca gcgtcgaaga
     1321 tcgatctcct taatggcgag caccatgtcg gtgggagctc cacgccatcc gcgatcgccg
     1381 gttatccgat cgttaccgtt cccgccggat atgctgccgg cttgcccatc ggcctctcct
     1441 tcttcgggac agcgtattcg gaaccgacgc tgatccggct cgcctacagc tttgagcagg
     1501 ctacgcaggt gcgccacgcg ccagcctata cagcaccggg ggtattgccg cctgagcacc
     1561 agtaggcaag tcaggccgcc tgtgcagggt ggaaacgtct tcaggcgtat cgggaacgat
     1621 ccgcgggcag cgaccagacg ggatcaaggg cggaatcccg gcgtgcgggt atgctagagg
     1681 gtgaggagac cgtggcgatg tagccgcgac tccctgtgcc gagcgataca cgaacaggtc
     1741 agggtacatg caggaccgtc caccacccac cgatccggat gagcggcgcc catcatccga
     1801 ttctggcgtg accccggatg atgtcggcga tgcgccggaa ttggatcagc cggacgagcg
     1861 actctcgccc gatgtcgatg ccgaaccccg gcaggtcagg gtgaccccga ccgcgcgacg
     1921 ccgcaatgca ccacaacccg gaccggctca agatgagcgt gatcgggccg agccagtcga
     1981 ggcgccagcc gcctatacgc cggggttgga ggggcagccg caggccacag cggcacgcag
     2041 ccggccgtgg acctggccac tgatcagcct cattggtttg cttgcggtgg ttttcgtcaa
     2101 ctggctcgcg aactggctgc cgctcaacga ccagacgacc ggtgaaatct cccgggagaa
     2161 cccggttccg tttcagccag ccggttgggc cttcctgatc tggccgttga tctacgcagc
     2221 gctcttcgcc ttcgtgatct acggcttctt gcccgctggc cggcgtaact cccgggtccg
     2281 ggccatcgga cccgttttcc tggtcgcgaa catcgcgaat attgcctgga ttttcctctg
     2341 gcactgggag cggttcgcgg cctcatttgt cgcgatcgtt gtgctgctcg ggagtctcgc
     2401 ggtcatctat gcgatgttgc cgggacctcg cgggaacctg tcgagcgtct cgcgggtgca
     2461 acgcctgctt gtccgtgtgc catttagcct ctatctggga tggatctcga tcgcgaccat
     2521 cgctgccctt gaggtgtgga tgatcaatgg tggctggaac ggtggtccct tcgggctgcg
     2581 cggctggaca gtggtttttc tcattgctgg cgtgctcgtg gctgcggtgt tcgcctttgc
     2641 cgcgaaggat gcgatctacc cccttgtctt cgcgtggggc tatttcgcta ttgcctacga
     2701 gcagtgggat ggttccagcc tcattagtat cctggcgggt tcgctggcga ttgtcgcggc
     2761 cgtgctgacg gtcatggctg tcatgctctc ctttggggca cgtgctggat cagcgctgcc
     2821 ggcaatgccg cgacaggtcg acaagccgcc aactgctcca tgacgtttgg tcccttgcgg
     2881 gtctgccagt tccggtttcg ataatgacaa ggagtatcca tgccccgaga actcgtcgcc
     2941 gttgcgccgc gtactccgca attgcgggag tacgaggagc ggccacctgg tccccacgac
     3001 gttcgtgtcc gcaccgagat tgcatcgccg aagcatggga ctgagctggt cgcctaccgg
     3061 aatgatccgg tcgccagccg tccctacgat tcgctgtggg gggcggtggt cccatcagct
     3121 gcagacgatg cactcgcccg cttcccgatg cggcttggaa acatggcggt tggtgtggtg
     3181 acggacatcg gggcggaggt ttcccgtttc accgtcggcg accgcgtgtt tggccatttt
     3241 ccgattcgtg aaacacagac agtcgatgag atgcagacag atctcctccc gcctggcctg
     3301 agtgcagaag cagctctctg tctcgatccg gtcgtcatgg cgttggcgat gcgggatggc
     3361 gggatcaagc tgggggaccg cgtggcggtc ttcgggctgg gagccattgg gcttatcgcc
     3421 atccagctcg cccgcctggc cggggcggcc caggtcatcg cgctcgatcc cctggagaac
     3481 cggcgcaacc tggccctggc gctcggcgcg gacatcgtgc ttgatccgac cgcgcacggc
     3541 ggtgatgcgg gcctggagat ccgaaagctg accggcgggg acgctcccga gcccgagatc
     3601 ccgacatcgg cgcgcatcat cggcggctac tccgaggctc cgacgcagtt gaccgagctc
     3661 ggagttgatg tcgcagtaga aacgtcgggc agcattccgg cgctgcaaca ggcgatccgg
     3721 gcaaccaggt tcggagggac cgtctgcgtc gtctcctttt atggtcggga ggcggcgggg
     3781 ctgctgctcg gcgaggagtt ccatatcaac cggctgcaac tggtctccgt gcgatcgcag
     3841 agcctaccaa tgcgggatgc gccggcctgg accctggagc gcgtggtcgc gctggggctg
     3901 gactggctgg ttgaagggcg catccggacc gaagggatta tcacgccaat cctgccgttt
     3961 gaggagagtg tggacgccta tcggatgatc gacgagcggc ccgcggaatc gatcaaactc
     4021 ggaatccggt tttcctgaac cgcgacaggc ccctcgtacg ggcgatctcc gtgatcgccc
     4081 tcgtttcatg ggaagtgcca acgggtgaag accaacttcc catcatcccg agccggtgag
     4141 gtcgcgccct gcaagggggt tagcggccga accgtgtcga gggatcttgg ctccgggccg
     4201 gcgtcctgat tcgcgagagc aaggattcct cgactacgcc cggaatgacg gggtcttgtg
     4261 cgtgaggggt atgcccatgc gaccaacgcg tctcgcctga gtcatacgcc tacccacaag
     4321 aatcccgcct acgcctcctc ccactccttc cggatgtggg cggcggtggt gcgcatcgat
     4381 gtatcgtagt cgccactgac acgacactcg atgccgagcc agccgtcgta gccgatgtct
     4441 ttcagggcgg caaaaccggg ctggaaatcg agatgcccct gccccggttg cagacgattg
     4501 ctatcggcca cgtggacgta gacgatgtga ttcgcggatt cgtggatgct tgccgcgatg
     4561 tctgcctcct caatgttcat atggaagaaa tccgccatgg tggcgacggg tgctcccccc
     4621 gcttcacggg cgatctccgc gccttgctcg acgcggttga cgatgtaggc ctcgtagcgg
     4681 tttagcggct ccaggaagag gtggacaccg gcctcccgcg cggtgggcgc gagttcccca
     4741 agctggcaca gcagcagctc gcgttccagc tcggcgccgg tcttccatgg cgagagatcg
     4801 tgcagggccg cggtgcgccc gaattgcggc acaacaagca cgccgacggc tcccagctcg
     4861 cccgcaagtg tcaggcgctc gcgcgttcgc cgaaaggcat cctcgcgcac ggcgggatct
     4921 gcatcgagta gccgggggat accctcgatc gcggaggcca gcacggtgga acccgcgaag
     4981 gcgtcatgca gctcctcacg gggtagctca agagagccag cgtgcagctc gatgccatcg
     5041 atccccatcg tttccagcca ggcgagttgg tccgcaaacg tctcgccggg aaccattgct
     5101 tccctgatcg tcagtttcat ggggtctcct gttcatgtgg acagcgtgac agccgctgtc
     5161 tcattttcgc tatcctgaag gctaccagcc tgtcgccaag atgtggcaat tccctagatt
     5221 tgacatatat ttctctgggt cgcacgggat aagcatctgt tgcgaaaatc attcgggtgt
     5281 gagagcatga ggcttgtacc gctttcacaa acatacgttg ttccgttctc attgaaccgg
     5341 gagaagtgcc gtgaagcaac gacgttttac agggttctgg acgatcgcag cgtgtttcct
     5401 gcttgtgctg acgagcattg gcctgcagcc agcagctgct caggaaactg tcgagctgac
     5461 ggtatgggat acgcatacgg gagaagtcga aaacgcagcc gcagaccaga tctatggcgc
     5521 gtttatggaa gcaaacccga atatcgagat cacccgtgaa gtctattccg tggataacat
     5581 gcggcagacc gtcaacacgg ccatcgcgtc ggggaccggt ccggacatca tcttctacga
     5641 tgctggccct ggatacgcgg gtgttctcgc ccaggcgggt ttgctcacgc cgctcgatga
     5701 gtacgccgag cagtatggct ggggcgaacg gattgccgag cagtcgcttg aggcgacgac
     5761 gctcggtggc gagctctacg gcctgccgct ccaggtcgat ctcatcggca tgtactacaa
     5821 ccagacgctg atcgaggagg cggggctcac ggtcccgcag acgtatgagg aactcctgac
     5881 cttctgccag ggtgccagcg aggctgggta tgtcccgctc gcgtttacga ataacccggg
     5941 atggcagggc ttccaccagt tctcgatgat cgtgaacaac atgctaggcc ccgattgggt
     6001 gcaggagaag ctgctgaaca atcagggcga atgggattcg cccgaggttg tcgaggcgat
     6061 caactcctat ttcgtggaga tgcgcgatgc cggctgcttc agctcagatg tcaacgcact
     6121 gacgaacgat gacgccaatg cgctgttcca gtcgggcgag gcattgatgt ggccgaccgg
     6181 cagctggatg gcaagcggtc tcaactcgga gaccatgccc gacatggagc tgggatacat
     6241 gccgttcccg gcgcttgaag agggcgcggg atccttctgg gtctcgggtg tgggctctgc
     6301 gtactacatc acggagacca gcgagcacaa ggacgaagcc gctctcttcc tggactacct
     6361 cttctcgccg acggtcgctg cgcagtgggt ggacgaagcc gggttcttcg tgccgatggc
     6421 cgtggataag gacacgctgg agctgacacc gctccaggac gcgatcctga ccgagcttca
     6481 gcgcgcggcg gcgggcgaga tcgagttggg ttacaacatc gacgtgctgg cacccccgcg
     6541 attcaacgaa acccttcaaa gcggtatcca ggccgtgctt gccggtgaca tgacggcaga
     6601 ggagcttgcc acccggatcc aggcggcgtg ggaggaaagc tggcagggac agggcacccc
     6661 ggaggcatca ccggcgtcat aacagggcgc agggtggctt gaacgggatg acgcgcagac
     6721 gagggtgggg ctgatgccct gccctcgtct ggctcagggg agggagacat cgcgatggcc
     6781 gtgatggagg cagcgccttc ggacactcgt agccatcggc tgcgacggag ttggactgag
     6841 tacagggctg gcattctctt cctgttgcca gggtttgtgc tctacgcgat cttcatgatc
     6901 tatcccttct ttcagtcgat ctatctcagt ttcaccgact ggaacggagc cgagccggtc
     6961 aagaacttca ttgggctgga gaactatcgc aatctcctga ccgataacct gctctggaaa
     7021 tcgctccgga acaacgtgat ctgggttgtg atcggcacgc taggtccatt ggccctggga
     7081 ttcctgctgg ccgtcctgct tgccagtcgg cccaaagggt ttaccctctt ccgtacggcg
     7141 tacttcatgc cgcaggtcct ctccccggtc gtgatcggca tcgtctgggg atggatgtac
     7201 aatccgttgt tcggcatttt caatgtggcc cttgagcggg ttgggcttga aagctggagc
     7261 cggggttggc tcggcgatcc ggactgggcc ctctacgctg tcctgctcgc cgcgatgtgg
     7321 gccgagactg gctttgtctt cgtggtcttc cttgccggat tgcagaatgt cagccgtgac
     7381 ctgattgagg cggcgacgtt ggacggcgcc aatgcctggc ggcgcttctg ggacgtgacc
     7441 gtgccacaga tggcgaacgt cgtgaacgtc gtgacagcgc tgctcctgat cggcggctt
//
LOCUS       DASYWM010000151         9388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_625445, whole genome shotgun sequence.
ACCESSION   DASYWM010000151 DASYWM010000000
VERSION     DASYWM010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625445"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2225)
                     /locus_tag="VGR29_06165"
     CDS             complement(<1..2225)
                     /locus_tag="VGR29_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2073212.1"
                     /translation="MSRTSGLDSIRTGQTVPADDTWDRATWSLPAPLTPLVGRMRELA
                     AATDQLQRPGTRLLTLTGPGGVGKTRLALEIANRMRHSLPDGAVFIDLTSATNPSMVP
                     IVIAYGLQMSVRDMDALPGRLVRELGDREMLLVLDNFEHVVRAGPDIAELLALCPLLK
                     VLVTSRMPLHVRGEQEIPVPPLEVPSDRQATDLKSLGEIDAVRLFVQRAQAVQPGFRL
                     TEQNAADVAEICCRVDGLPLAIELAAVRTKLFPVKALRERLSDRMALLTGGARDLPER
                     LQTIRHAIQWSFDLLSTHEQELFRGLSIFMGTFSIEAAKRVAAWEGTAEANLDEDLID
                     GLQSLLDKSFLIQETSVEGDARFRMLEIIREFGFEQARLAGEDAHFKRRFLDYYTEMV
                     APMEEELIGPNQELWLQRLDEEFPNIEFALQCGLDLDVEAHNQGLILATSMWRYWTSR
                     GRFASGTRWLRQMLDQPSTVGPSVRARALNNLGNLVFELGQHVAATEYYEESRQIYEA
                     AGDVNGVADALNNLGMIFVHNGDLEAALNAMRQSMEIRKVTGDSLALPTTLSNLGDMA
                     IFQGDFDQAERYHREAYDLRCEIGNKRGIALSCYNLGTLAILRRDWDAARNWHERGLK
                     SANEIDDAYSQACLNLGRGVVANHRGDVAMAVASLTAALRSFRDMETRRMLIEALDVI
                     GLVAARHGHDTESARLLGASTELRRQYPLGALARRAQWVHDIVAGLRQRIGDEAWAAQ
                     TG"
     gene            2446..3894
                     /locus_tag="VGR29_06170"
     CDS             2446..3894
                     /locus_tag="VGR29_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2073213.1"
                     /translation="MSDFRARQASTPSSSMSRRSVLKRGSAMGVAAALASRMDAFASA
                     GGRFPNGSATLYQATPSVDISGTELKILQWQHFVPSYDAWFQGFVNEWGTANGVTVTL
                     DQVNTADIPAAFAAEVAAEQGHDLIEHIAPLAQFEESVIDLSDLWDEATSRFGEPVEN
                     SRLNSYNPTTDKYYAFVHGYAPDPANYRKTLWETVDLPNGPTTLDELLDGGTKIWNEQ
                     GVQMGLGMSQEIDSQMAAQTLIWAFGGAIQDENENVIINSPETVEAVNFMKELFQNCM
                     TPEVFSWNAASNNQLLAAGQASYILNSISAYRTAQETQPEVALDIFFNTPLVGPAGEE
                     RALAHAHASMGYYIPTYSQNQDTAREFILHLVANYEAAALASKLYNFPTFSETVPGLD
                     DALASDPAGSEPVDKLLVLKDAADWTVNLGWPGPANAMAGQAQTTFILSNMMAKAARD
                     EMSAEESVAEAEAQLNEIAETWRSRGLMGGGQ"
     gene            4024..5094
                     /locus_tag="VGR29_06175"
     CDS             4024..5094
                     /locus_tag="VGR29_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2073214.1"
                     /translation="MAIVETRNLSKHFGEGVVAVDDINIRTADGEFLVLLGPSGCGKT
                     TLLRMLGGLEQPTGGQILISERVVNHLPPRARQIAMVFQSYALYPHMSVKGNIAFPLK
                     AAKISKDEQKRKIEWASTILGIDHLLDRKPGQLSGGQRQRVALARALVREPNAFLLDE
                     PLSNLDAQRRASARDELQIFQREIGTTTIYVTHDQVEAMGMGHRIAVINHGHVQQLGT
                     PREIYEEPTNTFVATFLGSPPMNLIAHDTTLIGFRPEALVPVEDAGDPDPSTWLEFTL
                     GNIREEYLGSEKLVYGEIAGTRAVSRLAANHPISHGDGASGQFMVKRSDIRMFDAESG
                     QAITAVPPQVVTARAARTRVAS"
     gene            5091..6059
                     /locus_tag="VGR29_06180"
     CDS             5091..6059
                     /locus_tag="VGR29_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2073215.1"
                     /translation="MSVSTATVAPVQKEKRERYVLENERVLGPLLLLPAVFYIFALVG
                     LPLVLAVLYAFSTASAGNPRIESLDDFVGLRNFRAVLDDGVFLKALRNTIFFTLGSQA
                     IVIVLANILALVLSADFHGKRLVRFLIMLPWTTPIALSVLGWFWLFDSVYSPIDWVMR
                     EVGLLGPDSTFGPARNMYWLGKTALAQGAVLLIHVWRTLPLATVILLAGLTAIPKDIS
                     DAADIDGAGFWRQLFYIRLPLLLPILAIAMLFSMVFTFTDLTVSLVLTRGGPVNDTQV
                     LSSWAYFKGIQGGDLAQGAAVALFMLPVLIAIAIAMLRLARRGGQG"
     gene            6061..6957
                     /locus_tag="VGR29_06185"
     CDS             6061..6957
                     /locus_tag="VGR29_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2073216.1"
                     /translation="MSAPNARTPVVEEEAGGPSRLKHLAGRGMLYSLAIFFALFGALP
                     FAWMVLTVFKTNTDLYKPQNNPFVYNDPPTLSNLDVLWNQTNFPTFVGNTLLIATLVV
                     IITVIIVVPAAYSLVRLSSRWGESLGIMIFLVYLVPPTLLFIPLTGIVADVGLQNSKF
                     SMVLVYPTFTIPFCTWLLMGFMRNFPWEIEEQAMIDGYTRLQAIRKTVLPVLVPGILT
                     VVIFSFTLTLHEFVYALAFVQSSAEKTISVGVFTELIRGDAYFWQSLMAAAAVVALPV
                     AIVYNLFLDKFIAGFTLGAVKG"
     gene            7470..8420
                     /locus_tag="VGR29_06190"
     CDS             7470..8420
                     /locus_tag="VGR29_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEV2073217.1"
                     /translation="MAAEIIPIPPSRPTFADVPRCKDLESLAAHIAIIGVPYGVPASM
                     ESSRAPSSTAPAAIREESLRFGKYLTHHDIDLGGPLLNGRDIRIVDCGDVAMTPGDLF
                     GNSAATTSVIRKICSKGVVPIILGGDHSIPIPAMRAYDNAGSMCLVQIDAHMDYRDEV
                     NGVKEGLSSSIRRASELDYVNGIVQIGLRGVGSAREQDVMDALEHRNIIISAAEINHQ
                     GVDAAVKRIPPSDRFYITLDMDGMDPSIAPGVNSPAFGGLDYWQATGLLWAVAKQGRV
                     VGMDMVEIAPHLDTRGLTSMLAARLILNLIGAMARSGQLR"
     gene            8785..>9388
                     /locus_tag="VGR29_06195"
     CDS             8785..>9388
                     /locus_tag="VGR29_06195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073218.1"
                     /translation="MDTLGTVLLLFAVGAIIAFPISGRSTSTRGSAPVTTFFGLFFCV
                     TVPMLTFSPNVWWLALSLTLYGLANGGVDVAMNAQGVEIELATGKKIMGSLHGFYSLG
                     GLIGAGTGALAAWLDTRLEWHFTTLSVLGFGIMAWAATGLIQDRTREGTKPESGGFVI
                     PPRILWPLGIIAFCSALGEGAMADWSALYLHEHLGSRESVA"
BASE COUNT         1801 a         2969 c         2731 g         1887 t
ORIGIN      
        1 ccagtctggg cagcccaggc ctcatcacca atccgctgac gcagaccagc aacgatgtcg
       61 tgtacccact gggcccgacg agcaagcgca ccgagaggat attggcgtcg aagctccgtc
      121 gatgcgccga gcaaacgagc cgactcagta tcgtgaccgt gccttgccgc aacgagccca
      181 attacgtcca gcgcttcaat cagcatccgc cgggtttcca tgtcccggaa ggatcgcaac
      241 gctgcggtca gggaggcaac cgccatcgcg acgtcacctc tgtggtttgc caccactccc
      301 ctgcccaggt tcaagcatgc ctggctgtac gcgtcatcga tctcattcgc cgacttcagc
      361 cctctctcat gccagttcct ggcggcatcc caatccctgc gcaggatcgc gagggtgccg
      421 aggttgtaac aagagagtgc gataccccgc ttgttcccga tctcgcagcg gaggtcgtaa
      481 gcctcccgat ggtagcgctc ggcctgatca aaatcaccct ggaagatcgc catatcgccc
      541 agattgctca acgttgtcgg aagcgccagt gagtctcccg tcactttccg gatttccatt
      601 gactggcgca tcgcgtttag ggcggcctcg agatctccat tgtgcacgaa gatcattccc
      661 aaattattga gcgcgtcagc gacaccgttc acatccccgg cagcctcata gatctgcctg
      721 ctctcctcgt agtattcggt ggcggcgaca tgctggccca attcgaatac aaggttgccg
      781 aggttgttca acgcccgggc acgtaccgac ggccctacgg tcgacggttg atccagcatc
      841 tggcgcagcc acctggttcc ggatgcaaag cggccccggc tcgtccagta tctccacatc
      901 gatgtcgcga ggatgaggcc ctggttgtgc gcctcgacgt ccagatcgag cccacattgc
      961 agtgcaaact cgatgttggg aaactcctca tccagacgct gcagccaaag ctcctgattt
     1021 gggccgatca gctcctcctc catcggcgcc accatctcgg tgtaataatc caggaatcgc
     1081 cgcttgaagt gagcatcctc gcccgccagt cgcgcttgct cgaagccgaa ctcgcggatg
     1141 atctccagca tccggaaccg ggcatcccct tccaccgatg tctcctggat aaggaaactc
     1201 ttgtccagaa gtgactggag gccgtcgatc aaatcttcat ccaggttcgc ctctgccgta
     1261 ccctcccatg cagccacgcg tttcgccgcc tcgatcgaga acgttcccat gaatatggag
     1321 agccctcgaa acagctcctg ctcatgcgta ctcaggagat cgaagctcca ttggattgcg
     1381 tgccggattg tctgcaaccg ctccggcaaa tcccgcgcac ctccggtgag gagcgccatc
     1441 cggtccgaca gccgctctcg cagtgccttg accggaaaga gtttggtacg gacggccgcg
     1501 agctcgatag caagtgggag cccgtccacg cggcaacaga tttctgccac atccgccgca
     1561 ttctgctccg tcagccggaa ccccggttgc acggcctgcg cgcgctggac gaagagacgc
     1621 accgcatcga tctctcccag gctcttgaga tcggttgcct ggcgatcgga agggacctcc
     1681 agcggcggaa ccgggatttc ctgctccccg cgaacgtgca acggcatcct gcttgtgacg
     1741 aggactttca ggaggggaca gagagcaagg agttcggcaa tatcaggccc ggcgcggacg
     1801 acatgctcga aattgtccag gaccagcagc atctcacggt cgcccagctc gcgcaccagg
     1861 cgcccgggga gcgcgtccat gtcgcgtacg ctcatttgta agccatacgc gatgacgatc
     1921 gggaccattg atgggttcgt ggccgacgtg aggtcaatga aaaccgcacc atccggcagt
     1981 gaatgccgca tccggttcgc gatttcaagc gcaagacggg tcttgcccac acctccaggg
     2041 ccagtcaggg tgagtaaccg ggttcctggc cgctggagtt gatcggtagc ggccgcgagt
     2101 tcccgcatgc gcccgacaag cggagtgaga ggggcaggca gcgaccatgt tgcccgatcc
     2161 cacgtgtcat cagcgggcac tgtttgacca gtccgtatgg aatccagccc ggatgtcctg
     2221 gacaaggtgc ttccttccct ggtgtggaga tgggtctgcc gcttcgttgc gcctgacggt
     2281 ttccttattc tataccacca tgcgattgcc cgtagtggta gcagcccggc tcctcactcc
     2341 ttcgcttgtg tcgcttgaca agccgtccat tctaaggaac actctcggaa cgtctgttta
     2401 tctacaattc aggtccattg ggacccagcc aggagcgaga tccacatgag cgatttccgt
     2461 gcccgtcaag cctctacacc atcatcctcg atgtccaggc gcagcgtgtt gaagcgaggc
     2521 tctgcgatgg gcgttgcggc ggcgctggcc agccgtatgg acgcgttcgc gtcggcaggc
     2581 ggccgattcc cgaatggttc agcgaccctc tatcaggcca caccctccgt ggacatttcc
     2641 ggaaccgagc tcaagatcct gcaatggcaa catttcgtac cgtcgtacga cgcgtggttt
     2701 caagggtttg tcaatgaatg gggcacggca aatggcgtta ccgtgacgct ggaccaggtc
     2761 aacaccgccg atatcccggc agcgttcgct gcggaagtgg cggccgagca gggccacgac
     2821 ctgatcgagc acattgcccc tctcgcccag ttcgaggagt cggtcatcga cctcagcgat
     2881 ctgtgggacg aggccacctc tcgcttcggt gagcccgtgg agaacagccg gctcaactcc
     2941 tacaacccta cgaccgacaa gtactacgcg ttcgttcatg gttacgcgcc tgacccggca
     3001 aattaccgca agaccctgtg ggagacggtg gacctaccca acgggccgac gacacttgac
     3061 gaactcctcg atggcggcac gaagatctgg aacgagcagg gcgtgcagat ggggctcggc
     3121 atgtcccagg agatcgactc ccagatggct gcccagacac tgatctgggc ttttggtggg
     3181 gcgatccagg acgagaacga gaacgtcatc atcaactcgc cggagacggt cgaggccgtc
     3241 aatttcatga aagagctgtt ccagaactgc atgacgccgg aggtcttctc ctggaacgcg
     3301 gcatcgaaca accagttgct ggcggctggc caggcctcgt acatcctcaa ttcgatctcg
     3361 gcctatcgca cggcgcagga aacacagcct gaggtggcgc tggacatctt cttcaatacg
     3421 ccactggtag gtccagctgg ggaagagcga gccctcgccc acgctcacgc gtccatgggc
     3481 tactacattc caacgtactc ccaaaaccag gacaccgcca gggagttcat ccttcacctt
     3541 gtagcgaact atgaggccgc ggcacttgcc agcaagctct acaacttccc gaccttctcc
     3601 gagaccgtgc cgggcctgga tgatgccctg gccagcgatc cggcaggctc cgagccagtc
     3661 gacaagctgc ttgtactgaa ggatgcagcc gactggaccg tgaaccttgg ctggccggga
     3721 ccggccaacg ccatggctgg acaggcgcag acgaccttta tactttcgaa tatgatggcc
     3781 aaggcggccc gcgacgaaat gtcggctgaa gaatcggttg cggaggccga agcgcagctc
     3841 aacgaaattg ctgaaacctg gcgaagccgg ggcctgatgg gcggaggcca gtagcacagg
     3901 aatagtcacc tgacgcacca ggtgtccagc cttgtgcccc ggagcacggc cccgagtctt
     3961 cggtggccgt gctccccgtt tcgccaccat acctccgaca gatgtacagg aattgttcaa
     4021 actatggcca ttgttgaaac acggaacctc agcaagcact ttggtgaagg ggtggttgcg
     4081 gttgacgaca tcaatattcg caccgccgat ggggagttcc tggttttgct cgggccctcg
     4141 ggctgtggca agaccaccct gttgcgaatg ctcggaggtc tcgaacagcc gacaggcggc
     4201 cagatcctga tcagtgagcg cgttgtcaat cacttgccac cccgggctcg ccagatcgcc
     4261 atggtcttcc agagctatgc cctctatccg cacatgtcgg tcaagggcaa tatcgccttt
     4321 ccgctcaagg cggccaagat cagcaaggac gagcagaagc gcaagatcga gtgggcgtcg
     4381 accattctcg gaatcgacca cctgctcgat cgcaagccgg gacagctctc cggcggccag
     4441 cggcaacgtg tcgcccttgc acgcgcgctc gtccgggagc ccaacgcctt cctgctcgat
     4501 gagcccctct ccaacctaga tgcccagcgg cgcgcctccg cgcgtgatga gctgcagatc
     4561 ttccagcgcg agattggcac gacaacgatc tatgtgaccc atgaccaggt cgaggcgatg
     4621 ggtatggggc atcgtattgc cgtcattaac catggccatg tacaacaact cggcaccccg
     4681 cgtgagatct acgaggaacc gaccaacacc tttgtcgcca ccttcctcgg gtcaccaccg
     4741 atgaacctga tcgcccatga caccacgctc attggctttc gcccggaggc cctggtcccg
     4801 gtggaggacg ccggggatcc ggacccatcc acttggctcg agttcacact tggcaacatc
     4861 cgggaggagt acctaggctc ggagaaactt gtctatggcg agatcgccgg cacgcgagca
     4921 gtgtcccggc tcgcagccaa tcacccaatc agccatggcg acggcgcatc cggccagttt
     4981 atggtgaaac gcagcgatat tcgcatgttc gacgcggaat ccggacaggc gattaccgcc
     5041 gtcccgcccc aggtcgtcac ggccagagcc gccagaaccc gggtagcatc atgagcgtga
     5101 gcacagcaac cgtcgcaccc gtacagaaag agaagaggga gcggtatgtg cttgagaacg
     5161 agcgcgttct cgggccactc ctgctgctcc ccgcggtctt ctacatcttc gcgctggtgg
     5221 gcctgccgct ggtactggcg gtgctctatg ccttcagtac cgcatccgca ggcaatcccc
     5281 ggattgagag ccttgatgac ttcgttgggc tgcgcaattt ccgggcggtg cttgacgatg
     5341 gggtcttcct caaggcgctt cgcaacacaa tcttcttcac actcggatcg caggcaatcg
     5401 tgattgtcct cgccaacatc ctcgccctgg tgctttcggc agactttcac ggcaagcggc
     5461 tggtccgatt cctcatcatg ctgccgtgga ccacaccgat cgccctcagc gtcctgggct
     5521 ggttctggtt gtttgactcg gtatacagcc caatcgactg ggtgatgcgc gaggttggtt
     5581 tgcttggacc tgattcgacg ttcggtccgg ccaggaatat gtactggctc gggaagacgg
     5641 cgctcgccca gggagccgta ttgctcatcc atgtctggcg aacactgccg cttgcgactg
     5701 taattctgct ggcaggcctc accgcaatcc caaaggacat cagcgacgcc gccgatatcg
     5761 acggcgccgg tttctggcgt caactcttct acatccggct accactgctg ctgccaatcc
     5821 tggcaattgc catgctcttc agcatggtct ttacctttac cgatctcacc gtctccctcg
     5881 tgctgacgcg gggtggccca gtgaatgata cccaggtact ctcgagctgg gcctatttca
     5941 agggcatcca gggaggcgac ctggcccagg gagccgccgt ggcgctcttc atgttgccgg
     6001 tgctgatcgc catcgctatc gccatgctgc ggctggcccg ccggggagga cagggctgac
     6061 atgagtgcac ccaacgccag aacaccggtc gttgaggaag aggccggggg gccatcgcgc
     6121 ctgaagcacc ttgccggccg gggaatgctc tactcgctgg cgatcttctt cgccctgttc
     6181 ggggcgctgc cattcgcctg gatggtcctg accgtcttca agaccaacac cgacctttac
     6241 aaaccgcaaa acaacccgtt tgtctacaac gatccgccaa cactctcgaa cctggacgta
     6301 ctctggaacc agaccaactt ccccacattc gtcgggaaca cgctgctgat cgcgacactg
     6361 gtggttatca tcaccgtgat catcgtcgtg ccggcggcct acagccttgt gcgtctctcc
     6421 agccgctggg gtgagagtct cggcatcatg atctttctcg tgtacctggt gccgccgaca
     6481 ctgctcttca tcccgcttac cggcatcgtc gccgatgtcg ggctccagaa ttcgaagttc
     6541 tcgatggtgc tcgtctaccc gacattcacc attccgttct gcacctggct cctgatgggg
     6601 ttcatgcgca acttcccctg ggagatcgaa gagcaagcga tgatcgacgg gtacactcgc
     6661 cttcaggcga tccggaagac cgtcctgccg gtactcgttc ccggcatcct gaccgttgtc
     6721 atcttctcgt tcacgctcac gctgcacgag ttcgtctacg cgctggcgtt tgtgcagagc
     6781 tcggctgaaa agacgatcag cgtcggggtc ttcaccgagt tgatccgtgg cgatgcctac
     6841 ttctggcaat cattgatggc ggcggctgcc gttgtcgcgt tgccggtggc aatcgtctac
     6901 aacctcttcc tcgacaagtt catcgccgga ttcaccctcg gtgcagtcaa gggatgatcg
     6961 gagacaacga gaggttgaac gcgggagtgg tgtccatgcc gctcccgcgt ctttgtgtcc
     7021 aggagaaaag aatgccgggt cgcgacatcg ccctacccgg ggtatagaag ggagagcgag
     7081 cctcatcgca cccggtgtca tcatgcatgg cagggattcg ggtacggtgg ggtggaggct
     7141 cgctggtacg gggaaatgca cgggacatgc caacgtctgt cttatgtggc atgcgtctcc
     7201 gcaacccggg ttcgatcacc cggtgtagtc acaaggctgg tcggccctct tcgcgaccgc
     7261 cggctcatcc aacgctcggg aacctcttca gtgagatggc agcgaatccc atatgccgaa
     7321 catcgcctgg ctcaacgtca agcatcgctt gaacgtgacg gaagccatca ccagcccgga
     7381 tatgcttgtg ttgcttccgc cgtgacaaca gcgcctttcg catggatcat aggcagctga
     7441 tgcagccgtc gcgatgcaag gagcacccgt tggcagccga gatcattccg ataccaccaa
     7501 gccgcccgac cttcgctgat gtgccgcggt gcaaggatct tgaatcgctt gctgcccaca
     7561 tcgcgatcat cggcgtgccc tacggtgttc ccgcaagcat ggaatcgtcc cgtgcgccct
     7621 cctcgacggc gccagccgcg atccgggagg aatccctccg tttcgggaag tacctcacac
     7681 atcacgatat cgatctcggt ggcccgcttc ttaacggtcg ggatatccgg atcgtcgact
     7741 gtggcgatgt tgccatgaca cccggtgatc tcttcgggaa cagtgccgcg accaccagcg
     7801 tgatccggaa aatctgctcg aagggcgtgg tcccgatcat tctgggcggc gaccactcca
     7861 tccccatccc cgcgatgagg gcctacgaca atgcaggctc catgtgtctt gtgcagatcg
     7921 acgcgcatat ggattaccgg gatgaagtga acggcgtgaa agaggggctc agcagttcga
     7981 ttcgtcgcgc ttcagagctg gattacgtca acggcattgt gcaaatcggc ctccgtggtg
     8041 ttggaagcgc gcgcgagcaa gatgtcatgg atgccctgga acaccgcaat atcatcatca
     8101 gcgcggcgga gatcaaccac cagggggtag atgccgcagt gaagaggatt ccaccctccg
     8161 atcgcttcta catcaccttg gatatggacg gcatggatcc atcgattgcg ccgggcgtga
     8221 actcccccgc attcggcggg cttgattact ggcaggcaac gggcctgctc tgggctgttg
     8281 ccaagcaggg acgagtcgtg ggcatggaca tggtggagat tgcaccgcat ctcgatacgc
     8341 gcggcctcac cagcatgctt gccgcccgtc tgattctcaa cctgattggg gccatggctc
     8401 gctcaggtca actccggtga gggcaagcat cggaagcgta ttgggcggga tgtctcgact
     8461 aactgcatgc atcgtcaggc cgagtgaaga gatggactgt gacggcccca taacctgctt
     8521 cgcatgggag gacgtaggtt tgcggcatac cgcgagtgtc acagatgcaa tccagagccg
     8581 agagctttct gtacccgcat ccctgagggt caagtcgggc atggggtgcc ggactacgga
     8641 caggcggctc accgcgtgag accgccactt cgcatcagac cgtagcctcg ccttggcgtc
     8701 tccctcttct tcgggatcaa tggcttcctc ggggcgaatt gggtcacgcg catccccgcg
     8761 gttgtccgga agctggagat cggcatggat acgctcggta ccgtgctgct gctcttcgcg
     8821 gtgggagcca tcatcgcctt ccccatctcc gggcgctcca ccagcactcg cggcagcgca
     8881 ccggtcacca ccttcttcgg cctcttcttc tgcgtcaccg tgccgatgct gaccttctcg
     8941 cccaatgtct ggtggctcgc gctatcgctc accctctacg gacttgccaa tggtggtgtg
     9001 gatgtcgcca tgaatgccca aggggtggag atcgagctgg ccaccggcaa gaagatcatg
     9061 ggctcgctcc acggcttcta cagtctcggt gggctcatag gggcagggac cggcgctctt
     9121 gccgcatggc ttgataccag actggaatgg cacttcacga cactctccgt gctcggcttt
     9181 ggcatcatgg cgtgggccgc gacaggactt atccaggacc ggacgcgcga gggcaccaag
     9241 cccgagagcg gtggtttcgt catcccgcca cgtatcctct ggccccttgg gatcattgcg
     9301 ttctgctccg cgctgggcga gggggcgatg gccgactgga gcgcgctcta cctgcatgag
     9361 cacctgggca gccgcgaatc agtcgcgg
//
LOCUS       DASYWM010000152         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_493438, whole genome shotgun sequence.
ACCESSION   DASYWM010000152 DASYWM010000000
VERSION     DASYWM010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493438"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VGR29_06200"
     CDS             <1..558
                     /locus_tag="VGR29_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HEV2073219.1"
                     /translation="SFSPRAIGYGFGQKELPWPNALRIWIGEIEANEDAENGEILLET
                     NIDDMNPQFVELVMERLFQHGALDVWTTAISMKKGRPATQISVLAPTGRRQELESVLI
                     ENTTTLGVRVIQIERTKAAREIETISTRWGDVRIKSRIWNGRIIDISPEYDDCVALAR
                     EHDVPIRVVWNETYRIGEARVGQKR"
     gene            complement(623..2251)
                     /gene="murJ"
                     /locus_tag="VGR29_06205"
     CDS             complement(623..2251)
                     /gene="murJ"
                     /locus_tag="VGR29_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEV2073220.1"
                     /translation="MRANAPDASVAPPGKGIALAAIVLMLGNVLSRVLGLVREQLAAG
                     LFGTGDAIAAFTIADNIQTLLFDLLASGALQAALIPVLAQWAAPESRSREELRRIGGT
                     LVTGVFVVVGVLVVVGIFFASRVVDGLVWFSGDTEARGPETVELTIRLVRIVLPAALL
                     LSIATVLQAILYSIEKVTAPALSTAVRNAMIVIFTALFGSAWGVEAMAWGTLVGALAI
                     VLIQIPPLVRNGVLPRPAFDIHHPALRQMGLLYIPVFLGLLVNSLAVVVDRGLAWGAG
                     EYAIGAMRYATTLVQLTLGIIAAAISLAALPTLSRHFQTGDTVAYRTTLGRALVMVTV
                     LIFPATFGLAALASPIAELLFGYGATGDEGLRHISLALLGYLPGTLAAAYDQVLIFAF
                     YARQNTRLPVVIGVMAVLVYFAVAFSLVDAFGMMGLVIANSAQFIAHTLVIWHFGRKR
                     FGWLASPRLSFLIPRCALAAGVAAGAGWALWMGLVHLVPETTGMADVGVRMALVGGPI
                     LLVAAIYLPWVARLCPREVAMIRTAIAQRLPGGR"
BASE COUNT          552 a          743 c          701 g          356 t
ORIGIN      
        1 tccttttcac cacgcgcgat tggctatggc ttcggccaga aagagctccc ctggccgaac
       61 gcacttcgga tctggattgg cgagattgag gccaatgagg atgcggaaaa tggtgagatc
      121 ctgctcgaga ccaacatcga cgacatgaac ccgcagtttg tcgagcttgt catggagcgc
      181 ctgttccagc acggcgcact cgacgtctgg accacagcaa tttcaatgaa gaagggtcga
      241 ccggcgacac agatcagtgt tctggcaccg accgggcgtc ggcaggagct ggagtccgtg
      301 ctgatcgaga acaccaccac actgggagtc cgcgtaatcc aaatcgagcg gaccaaggcc
      361 gcacgggaga ttgaaactat ctctacacgc tggggagatg tccgcatcaa gagccggatc
      421 tggaatggcc gaatcattga tatctcaccg gaatacgatg attgtgtcgc cctcgcccgc
      481 gagcatgatg tgcccatccg ggtggtctgg aatgagacct accggatcgg cgaagcccgc
      541 gtcggccaaa aacgctaggc atggcgacga tgatttccgt tcaagattgc cttggggctc
      601 tggcgacagg tgtcaggccc gatcaccgac ctccgggcaa ccgttgagca atcgccgtgc
      661 ggatcatcgc cacctcccgc gggcagagcc gtgcaaccca gggaagatag atggccgcaa
      721 ccagcaggat gggcccgcct accagggcca ttcgaacccc gacatcagcc atgccggtcg
      781 tctccggtac gagatgtacc agtcccatcc aaagcgccca tcccgccccc gcggcgacac
      841 cggcagccag ggcgcaccgt ggaatgagga aactcagtcg gggtgacgcc agccacccga
      901 accgcttccg gccgaaatgc cagatcacga gcgtatgggc aatgaactgc gccgagttcg
      961 caatcaccaa ccccatcatc ccgaaggcgt cgacgagtga gaacgcaacc gcgaagtaaa
     1021 cgagcaccgc catcacgccg atgacaaccg ggagccgcgt gttctgcctt gcataaaagg
     1081 caaaaatcag gacctgatcg tatgccgccg cgagggttcc tggcaagtat ccaagcaagg
     1141 ccaacgagat atgtcgcaac ccctcgtctc ccgttgcacc gtagccgaag agcaactccg
     1201 cgatcggcga ggcgagtgct gcgagcccga acgtcgccgg aaagatcagc accgtcacca
     1261 tgacaagagc ccgtcccagc gtcgtccggt acgcaacggt atcgcccgtc tggaaatggc
     1321 gcgaaagcgt tggcagggcc gcgagggaga tcgccgccgc aatgatgcca agcgtcagct
     1381 gcaccagcgt cgtggcatag cgcattgctc cgatggcata ctcccccgct ccccaggcga
     1441 gcccccgatc caccacgaca gcgagagagt tgacgagcag cccgagaaag accgggatgt
     1501 acaacagacc catctgtcgc aaggcgggat gatggatatc aaacgcaggc cgtggcaaga
     1561 caccgttcct gacgagcggc gggatctgga tgagcacaat cgccagagct ccgaccagtg
     1621 tgccccatgc catagcctcc acgccccagg ctgatccgaa gagcgcggta aaaatcacga
     1681 tcatcgcgtt ccggacagcg gtggagagtg ccggtgcagt gaccttctct attgaataga
     1741 ggatcgcctg cagcacagtg gcgatgctga gcagcaaggc tgccggaagt acaatccgga
     1801 caagtcggat cgtgagctcc actgtctccg gaccgcgggc ctccgtgtca ccggaaaacc
     1861 agacgagacc atcgacgacc cgtgaggcaa agaatatgcc gaccacgacc agcacgccaa
     1921 caacgacgaa tacccctgtc accagggtcc cgccgattcg acgcagctcc tcccgggaac
     1981 gggattcagg tgctgcccac tgggcgagaa ccggaatcaa cgccgcttgc agcgcgccac
     2041 ttgccaggag atcgaaaagg agcgtctgga tgttgtcggc gatggtgaaa gcagcgattg
     2101 catcaccggt gccgaagagt cccgcggcga gctgctcccg cacgagccca agcacccggc
     2161 tgagcacatt ccccagcatg agcacgatcg cagcaagggc gatccccttg ccgggaggcg
     2221 caacagaggc atcaggagcg ttcgccctca acggaagcgg atccaggcaa tcgcataggc
     2281 cagtgccata tagacgagat ccggcaccag gcgcacgcgc agggcacgct gggcatattc
     2341 gcccagttgg tt
//
LOCUS       DASYWM010000153         9130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_625518, whole genome shotgun sequence.
ACCESSION   DASYWM010000153 DASYWM010000000
VERSION     DASYWM010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625518"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VGR29_06210"
     CDS             complement(<1..608)
                     /locus_tag="VGR29_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073221.1"
                     /translation="MRRLFFGLLAALLLMPVSILSVLAAQDATPASGLADADMPQLDV
                     TLTATGYEGIPDQVEAGRYLVSLTIEEGASEFGGGVSFVQPPADMTAEDFLTAYMTPP
                     DESGVGEAAATPIEGGVATPAEGEGEMGGLPSFLFEATYAGGTYSFAGGVTEVVLDLT
                     PGEWIAWTDDFEAPQEPVFFEVTGEMPAELTEPESSATITMG"
     gene            1298..1513
                     /locus_tag="VGR29_06215"
     CDS             1298..1513
                     /locus_tag="VGR29_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073222.1"
                     /translation="MTDPVSRQMHDRGAKARENLVNALHECGELADAVATFNGDELLE
                     VLDYLDSLRFVMAESGQLLQGVVRGSL"
     gene            1514..1891
                     /locus_tag="VGR29_06220"
     CDS             1514..1891
                     /locus_tag="VGR29_06220"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEV2073223.1"
                     /translation="MPTWQWFLIVIGIVIVLGSAAIATWIWRSGLTAASAAAELRAIG
                     LDLARLPGRLRRLAADERVPRKARWLLIGLAIYIASPIDPIPDFLPIIGHLDEVILVP
                     IILVRVRRMIPADVWDEYFPKRS"
     gene            complement(1892..3376)
                     /locus_tag="VGR29_06225"
     CDS             complement(1892..3376)
                     /locus_tag="VGR29_06225"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEV2073224.1"
                     /translation="MVSDRDVRIGIGGIAIESSTFSPLLSTQENFLILRGDEIATSYP
                     YFESGTFEGRDDVTWLGTLKARSIPGGPVDAASYDAMKGELLERLEQVLPLDGFFFDI
                     HGAMSVVGMDDAEGDLITAIRDVVGPGCIISAGMDLHGNVSEVLVGVVDIFTAYRQAP
                     HVDAMETKARAVRHLLMLLDEGIRPLRAWVRIPVVLPGERTSTFWEPGKTVYGKLTES
                     DAVDGVLDASLWVGYVWADQPRASATTVVTGTDTDSIVSEAEKIARRYWDARQAFDFG
                     VPVGDADWTIDQALSLKQKSVIISDSGDNPTAGGAGDIPLMVERLLARPELASGERTA
                     LVAAVPNAEAVATAMGAGEGAEVTVTIGGVYDPVNGYSLELSGTVYSVYENDPVGGDI
                     VVIKSGGVHVIVPSLRKPYHHIREFENLRLNPKDHDITVVKIGYLEPELKDAASSAFL
                     ALTPGAVNQDIPALPFERLQRPIFPLDPEMSEPDLTPRIFGPIG"
     gene            3528..3686
                     /locus_tag="VGR29_06230"
     CDS             3528..3686
                     /locus_tag="VGR29_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073225.1"
                     /translation="MSQQTTKFLLDEDRILKTGYNEDQTYIYGTGAALAGVPMRDTFM
                     VTMETGGP"
     gene            complement(3811..4458)
                     /locus_tag="VGR29_06235"
     CDS             complement(3811..4458)
                     /locus_tag="VGR29_06235"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEV2073226.1"
                     /translation="MGSDGLTAAILVGGQSRRMGTNKALLTIDPNGSTIVETVARKLG
                     AVANEVVLVGSETAEYAFLDLPQIADMVPGIGVIGGIYSALVAAGRPHVLVVACDLPF
                     LNVSLLRYMASCPRDYDVLVPLLDQVQPLHAIYARTCLPLVEQSLRSGQYKVTGWFDH
                     ANVRTLEHSIIQRYDPTLLSTFNMNTPENFEFAKQVLASCLPAGTAPGVEPAHGD"
     gene            complement(4448..5035)
                     /locus_tag="VGR29_06240"
     CDS             complement(4448..5035)
                     /locus_tag="VGR29_06240"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HEV2073227.1"
                     /translation="MLNTSAALHDHLCPRQVLGVRMGMCAAELLDMDLPQRDKRLLSI
                     VETDGCFADGVSASTGCWLGRRTLRCVDYGKVAATFIDTLTSEAVRIHPSEASRRLVA
                     RYAPTAVDRWHAYLEGYQIMPTEELLVVESVQLVCSVEKLVSRPDHKTICATCHEEIF
                     NEREIVKDGRVLCRGCAKRAYFMPLPVSEGCQGGK"
     gene            complement(5096..5956)
                     /gene="fdhD"
                     /locus_tag="VGR29_06245"
     CDS             complement(5096..5956)
                     /gene="fdhD"
                     /locus_tag="VGR29_06245"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEV2073228.1"
                     /translation="MNLRPGSKTAARVWMIARDQGRLRRDELATEEPMEIRLVIHGVT
                     RTVAVTMRTPGNDFELAAGFLFGEGVIGSRDDLVQISYCVDPGIDAAQQYNIVNVTLR
                     HNAAPDLAPLERHFYTSSACGVCGKASLEALQLRSCPVIPPGPEIPVEVLYSLPDKLR
                     QAQGVYQKTGGLHAAALFDPNGQLVALREDVGRHNALDKLVGWALMEGRLPLSQNILL
                     VSGRSSFEIMQKSLAAGIPIVGAISAPSSLAVDLAREFGMTLVGFLRDERCNVYTSYE
                     RILMPSSPSA"
     gene            6184..8571
                     /locus_tag="VGR29_06250"
     CDS             6184..8571
                     /locus_tag="VGR29_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEV2073229.1"
                     /translation="MTTSDRPRTELQSARDNAPRDAEEVIHGQGTPLSPAAGCGPVMD
                     RSPKKSLYHHATAGWGAALNVGKILFHQRAMREAPGILFRMNHEKRGFDCPGCAWPDD
                     PKVRMDICENGVKHSTWEMSSKQVGREFFAAHTVSGLRQWSDFALEDAGRLTEPMVYD
                     PVTDRYMPIEWNDAFALAGSTLRGLDSPHQAAFYTSGRLSNEASFLYQWFVREFGTNN
                     LPDCSNMCHEASGVAMRAAIGTGKGTVDLHDWERADAIILLGVNAATNAPRMLTYLAD
                     AHRRGAALVHINPIIEAAATRTIVPHEYADMARFRATPTSTLNIQPRIGGDAALVRGV
                     AKALFEMATDDPEAIDTGFLDRFTNGVEAYRATCEATNWTEIEQQSGVPQAQIRELAS
                     IYRNANAAIIAWCLGISQQEHGVDTIREIVNLLLLRGNIGRPGAGPCPIRGHSNVQGN
                     RTQGINHHPTEELLDRLATTCQITPPRGVGLDVVGTVQAMHDGKVKVFLGMGGNFALS
                     TPDTAYCFEALRRCDLTIQVSTKLNRSHLVHGRQALIMPCIARSERDIQASGAQSISV
                     EDSMSMVHLSTGKKEPASPHLRSELAIIAGLAQETLPNTRTPWEELVANYDLIRDHIA
                     DAVDGFEDFNRRVRQEGGFRIHQPARERIFLTTSGRAEFSMAPLVDATPEAGRLVLST
                     MRSHDQFNTSIYSNDDRYRGVKNLRTLLFMNAVDMEERGLAEFDRIDITSFARDGSSR
                     SVYGYLAVTYDIPPGCAAGYMPELNVLCSIGDFSDKSRQPMMKHIPIDVTPSQAL"
BASE COUNT         1839 a         2907 c         2645 g         1739 t
ORIGIN      
        1 tcacccatcg tgatggtggc gctggactcc ggctcggtca gctccgccgg catctcgccg
       61 gtcacctcga agaagacagg ctcctgcggg gcttcgaagt cgtccgtcca ggcgatccac
      121 tcgcccggcg tcaggtccag cacgacctcc gtcacaccac cggcaaagga atacgtgccg
      181 ccggcatacg tggcctcaaa gaggaaggac ggcaagccac ccatctcccc ctcaccctcg
      241 gcgggggtgg cgacgccacc ttcgatcggc gtggcagcgg cctcgcccac accgctctcg
      301 tcagggggcg tcatgtaggc cgtgaggaaa tcctcggcag tcatatccgc cggtggctgc
      361 acgaaggaga cgccaccgcc aaactcgctg gcgccttcct cgatcgtcag agagacgagg
      421 taccgaccgg cctcgacctg gtccgggatg ccctcatagc cagttgcggt caacgtgaca
      481 tcgagctggg gcatgtctgc atcagcgagc ccgctggctg gagtcgcatc ctgagcagca
      541 aggacagaca ggatgctcac gggcatcagc agcaaggcgg caagcaatcc gaaaaagaga
      601 cggcgcatag gtttctcccc ctgtaatggc gaaacgtggc cataaaaata gagggacgac
      661 acgaacacgc acaggctcgc cggcgccggc accacctgca tgccatgtcg tcccctacca
      721 tcgtagacgt caggtgttcc aaaagtgttc cagacccatg gttgggggct ggcccctccg
      781 gattccggtg ccgctgcttc tcagacgaca gccgtcctga cggttctcgt tcaccgtttg
      841 gggtttaatc ccgaggtgcg aaaacgccgg acgaagggga cgtccggcgt cttcctgtgg
      901 gtggaggaag ggggaataga ttgtcgtccc tgtcggactc cggcaacgga gcctctcctc
      961 gacaggacat agaccgcgcg catcaggatc cagcaccaca caggatcatg gtgatgtcac
     1021 gtccagcgac tcgggagagg atccgctggc accacggtgg ctgggtccgt gttcggtgcc
     1081 gggaggcctc cccgtcgtcc ggaagggtca cccctagtat gtcgggctcc cctcctgagg
     1141 gcatccgtaa aagtacgtat ttgcccgccc acgaattgcg gagaacctac gtaatcggga
     1201 ccggatcgct ctttcatgga agcgagaccc ttccggagga cgttttggta aggtttccag
     1261 ccaaggcaac ggttcgtata tcgaccaagg acccaatatg accgatcctg tttctcgaca
     1321 gatgcatgac cgcggcgcca aggcgcgaga gaatctcgtc aatgctctcc acgaatgcgg
     1381 tgagttggcc gacgccgtcg caacgttcaa tggcgatgag cttctcgaag tgctggacta
     1441 tctcgacagc ctccgcttcg tcatggcgga gtcgggtcag ttgctccagg gcgtggtgcg
     1501 aggatcgctc taggtgccga cgtggcaatg gttcctgatc gtgatcggca tcgttattgt
     1561 cctcggcagc gcggccatag cgacctggat atggcgctcc ggactcacgg ccgcttctgc
     1621 cgcggcggaa cttcgcgcca tcggactgga tctggccagg ctgccaggtc ggctccgccg
     1681 cctcgctgca gatgaacgcg tgccgaggaa agctcgttgg ttgctgatcg ggctcgccat
     1741 ctacatcgca agcccgatcg acccgattcc tgattttctg ccgatcatcg gacacttgga
     1801 tgaggtgatc ctcgtgccga tcattctggt ccgcgtgcgc cgaatgatcc cggcggatgt
     1861 ttgggacgag tactttccaa aacgttcctg atcagccgat cgggccgaag atacgcggcg
     1921 tcaggtccgg ttcgctcatc tcgggatcaa gtggaaagat tggtcgctgc agccgctcga
     1981 acggcagagc cgggatatcc tgattcaccg ccccgggcgt cagtgccagg aatgctgacg
     2041 aggcggcatc cttcagctcg ggctcaagat agccgatctt caccaccgtg atgtcgtggt
     2101 ccttcgggtt cagccgcagg ttctcgaact cccggatgtg atgatagggc ttgcggagcg
     2161 aaggcacgat aacatggaca ccgcccgact tgatgacgac gatatcgcct ccaaccggat
     2221 cgttctcgta gacactgtag accgttccac tgagctccaa cgaatagccg tttaccgggt
     2281 cgtagacgcc cccgatcgtg acagtcacct cggcgccctc gcccgctccc atcgctgtgg
     2341 cgaccgcctc cgcattaggc accgctgcga cgagcgctgt tcgctcccca gatgcaagtt
     2401 ccggtcgcgc cagcaggcgt tccaccatga gcgggatgtc ccccgcccca cccgcggtcg
     2461 ggttgtcgcc cgagtcgctg ataatgaccg acttttgctt caatgacagc gcctgatcga
     2521 tcgtccaatc cgcatctccc accggcacac cgaagtcgaa tgcttggcgg gcgtcccagt
     2581 aacgacgggc aatcttctcc gcctccgaca caatcgaatc ggtgtctgtt cccgtcacca
     2641 ccgtcgtcgc gctcgctcgc ggttggtcag cccatacata tccgacccac agcgaggcat
     2701 caagcacacc atcgaccgcg tccgattccg tcagtttgcc gtagacggtc ttgcccggtt
     2761 cccagaaggt gcttgtccgt tcacccggca gcacaacagg aatccgcacc catgcgcgaa
     2821 gcggccggat gccctcatcc aggagcatca ggagatgacg aacagcccgc gccttcgtct
     2881 ccatcgcgtc gacatgcggc gcttgtcgat aggcggtgaa gatatcgacc acgccgacga
     2941 gaacctcgct cacgttgcca tgcagatcca tcccggcgga gatgatgcat ccgggaccca
     3001 ccacgtcgcg gatagccgta atgaggtctc cctctgcatc atccatcccc accacactca
     3061 ttgcgccatg gatgtcgaag aagaatccat cgagtggcag gacctgctcc agccgctcca
     3121 gcagctcccc cttcatcgcg tcgtacgatg ccgcatcgac cggcccgccc gggatggaac
     3181 gcgccttgag cgttcctagc caggtcacgt catcgcggcc ctcgaaggtc ccgctctcga
     3241 aatacgggta gctcgtcgca atctcgtccc cgcggaggat caagaagttc tcctgtgtgc
     3301 tcagcagcgg cgagaacgta ctgctctcga ttgcgatccc gcctataccg atccgcacat
     3361 ccctgtctga gaccacgtcg tgtccactcc tgttttcatg ccatcctcgg tacgtccgcc
     3421 ggtcacggta gcgtgaaacg gccagtcatg caacagcggg catcgacctg caatgcccca
     3481 atatgatcag ttgcgagata cgcgcgtttg gagaggggga gcctgaattg agccagcaga
     3541 cgacgaagtt cctcctggat gaggaccgca ttctcaagac tgggtacaac gaggatcaga
     3601 cgtacatcta tggaaccggt gcagcgttgg ctggggtacc catgcgggat acgtttatgg
     3661 tgactatgga aactggtgga ccatgattct gcgattcgcg ctcctattgg taatcgcgct
     3721 ccctgttgga gtcgttgagt ccggcgaggt cttcatccgc agataatgcc tttgcgatcg
     3781 acaggtcacg aaatcttgaa ggcgggtggc tcaatcaccg tgggccggct caacacctgg
     3841 cgctgtgccc gcgggaagac agcttgccag cacctgtttc gcaaactcaa agttctccgg
     3901 ggtgttcatg ttgaaggtgg aaaggagcgt cgggtcgtat cgctggatga ttgagtgctc
     3961 cagcgtgcgg acattcgcat gatcgaacca gccagtgacc ttgtactgac cagaccgcag
     4021 gctttgctct acaagaggaa ggcaggtccg cgcgtagatc gcgtgcaacg gctgcacctg
     4081 atccaggagt gggaccagta catcgtaatc gcgaggacat gatgccatgt agcggagcaa
     4141 gctgacattc aggaatggca agtcacatgc aacaaccagg acatgcggtc tcccggctgc
     4201 aaccagggcg gaatagattc cgccaatcac cccgataccg gggaccatgt cggcaatctg
     4261 gggcaggtca agaaaagcat attcggcggt ttccgatccc accaggacga cctcgttcgc
     4321 gaccgcaccg agcttccggg ctaccgtctc cacgatggtc gatccattcg gatcaatggt
     4381 cagcagtgcc ttgttcgtgc ccatgcggcg ggattgacca ccaaccagaa tggccgccgt
     4441 gaggccgtca cttcccaccc tggcaacctt cgctcacggg taacggcatg aaataggcgc
     4501 gcttcgcgca ccctcggcac agcactcggc catctttaac aatttcgcgc tcgttgaaga
     4561 tctcctcgtg gcatgtagca cagatcgtct tgtgatcggg ccgactcacg agcttctcca
     4621 cagaacaaac caactggacg ctctcgacca cgagcagctc ctcggtaggc atgatctggt
     4681 agccctcaag gtatgcatgc cagcggtcca cggcggtggg tgcgtaacgg gctaccaatc
     4741 gccgggacgc ttcggatgga tgaatgcgca cggcctcact ggtgagggtg tcgatgaagg
     4801 tcgctgccac cttcccgtag tcgacgcagc ggagggtgcg tcgacccagc cagcagccgg
     4861 tggacgcgga gacgccatcg gcaaagcacc cgtcggtctc gacgatgcta agcagccgct
     4921 tgtcgcgctg cggtaagtcc atgtccagga gttcggcggc gcacatgccc atccgcactc
     4981 caagcacctg cctgggacac aagtggtcat gcagcgccgc ggatgtgttc aacaagtgtt
     5041 ggagtcgttg ctccggcaca cgcggtcctt tccccgccag gacatctcgt atcgcctatg
     5101 cagaaggact cgaaggcatc agtatccgtt cgtacgaagt gtagacgttg catcgctcgt
     5161 cccggaggaa accgaccaat gtcatgccga attcgcgggc caggtcgaca gccagactgc
     5221 tcggcgccga tatcgcgccg acgatgggaa tgcccgctgc caggctcttc tgcatgatct
     5281 cgaagctgga gcgaccactg accagcagga tgttttgact gagcgggagc ctcccttcca
     5341 tcagggccca acctacgagc ttgtcgaggg cgttatgacg gccgacatct tcccgcagtg
     5401 ccacgagctg tccgttagga tcgaagagcg ccgcggcgtg caaacctccc gttttctggt
     5461 acacgccctg cgcctgccgc agcttgtcgg gaaggctata cagcacctca acggggatct
     5521 cgggtcccgg cgggatcact ggacagctcc tcagctgcaa cgcttccagg gaggctttcc
     5581 cgcaaacccc gcacgcgcta ctggtgtaga agtgccgttc cagcggcgcg agatccggcg
     5641 cggcattgtg ccgaagtgtg acgttcacga tgttgtactg ctgggcagca tcgatcccgg
     5701 gatccacgca gtagctgatc tggaccagat catcgcggct accgatcacg ccctcgccga
     5761 acaggaagcc cgcggcgagc tcgaagtcat tgcctggagt acgcatcgtt acggccacgg
     5821 tgcgcgtcac cccatggata acgaggcgta tctccatagg ttcttccgtc gccaactcat
     5881 cgcggcgcaa tcgaccctga tcgcgcgcga tcatccagac cctggccgcg gtcttgcttc
     5941 ccggacgcaa gttcacgctt ccccttcttc cgcgctcaat tccaatgagg atcgggatga
     6001 tatcttgcgg tgattgcgga gacgagtgta ccatctcgct cacctatgtc agatcgatcg
     6061 ataaacgcaa tacacctacg gctcgtccgg gtattgtaga tatacaattg gccacctaga
     6121 acccaacact taatgctccc ggtgccgcgc caaggccggc gagtgaagtc gcgagggaac
     6181 gccatgacca cgtccgaccg accacgaacc gagctccaat cagcccgcga caatgcgccg
     6241 cgcgatgcgg aagaggtcat ccacggtcaa ggcacccccc tatcgccggc agccgggtgc
     6301 ggaccggtta tggatcgctc gccaaaaaag agtctctatc atcatgccac cgcaggctgg
     6361 ggcgctgccc tcaatgttgg gaagatcctt ttccaccagc gggcaatgcg agaggcgccg
     6421 ggcatcctct tcaggatgaa ccacgagaag cgcgggttcg actgtcccgg ctgcgcttgg
     6481 cccgatgatc ccaaggtccg aatggacatc tgcgagaacg gtgtcaagca ctctacctgg
     6541 gaaatgtcgt cgaagcaggt tggccgagaa ttcttcgcgg ctcacacggt ctctgggctg
     6601 aggcaatgga gcgacttcgc ccttgaagac gccggacgtc tgacagagcc gatggtctac
     6661 gaccctgtga ccgaccgcta catgccgatc gagtggaacg atgcctttgc cctcgccgga
     6721 tcgaccctcc gcggtctcga ctcgccccac caggctgcct tctacacctc cggccgcctg
     6781 agcaacgaag ccagcttcct ctaccaatgg ttcgtccggg agttcggcac aaacaatctc
     6841 ccagactgct cgaatatgtg ccatgaggcc agcggagtcg ccatgcgcgc cgcaatcggc
     6901 accggcaagg gcacggtcga cctccacgat tgggagcgcg ccgacgcgat catcctgctc
     6961 ggggtcaacg ccgccaccaa tgcaccccgc atgcttacct acctggcaga tgcgcaccgt
     7021 cgcggtgcgg cgttggtgca catcaacccg atcatcgagg cggcggcgac ccgcaccatc
     7081 gtcccccacg aatacgccga catggcccgt tttcgcgcca ctccaacaag tacgctcaac
     7141 atccagcccc ggatcggcgg cgatgccgcc ctggtccggg gtgtggcgaa ggcgctgttc
     7201 gagatggcga ctgacgaccc ggaggcaatc gacaccgggt tcctcgatcg tttcacaaat
     7261 ggggtggagg cttaccgcgc cacctgcgag gcgaccaact ggacggaaat cgagcagcag
     7321 tccggtgtgc cccaagcgca gatccgcgag ctagcaagta tctaccgaaa tgcgaacgcc
     7381 gccatcatcg cctggtgttt gggaatttcg cagcaggagc atggggtcga tacgatccgt
     7441 gaaatcgtca acctgctgtt gctccggggc aatatcggcc ggccgggggc aggcccgtgc
     7501 ccgatccggg ggcacagcaa cgttcagggc aaccgaactc agggcatcaa ccaccacccg
     7561 acggaggagc tgctcgaccg cctcgccacc acctgccaga tcacgccacc gcgaggagtg
     7621 gggctggacg tcgtcgggac ggtccaggct atgcacgatg gcaaggtaaa ggtgtttctg
     7681 ggtatgggcg gcaactttgc gctctctaca ccagacaccg cctactgctt cgaggcgcta
     7741 cgccgctgcg acctcaccat ccaggtctcg accaagctca accgaagtca cctggtacat
     7801 ggccgccagg cactgattat gccctgcatt gcgcgcagcg aacgagacat ccaggccagc
     7861 ggagcccagt cgatctcagt cgaggattcg atgagcatgg tgcatctttc aaccgggaag
     7921 aaagagcccg cctcgcccca tctgcgctcg gaattggcca tcatcgccgg gctggcccag
     7981 gagaccttac ccaacacacg aaccccatgg gaagaattgg ttgctaacta cgacttgatc
     8041 cgggaccaca tcgccgatgc cgtggacggc ttcgaagact tcaatcggcg ggttcgccag
     8101 gagggggggt tccgaatcca ccaaccagcg cgggagcgca tcttcctcac cacctccggt
     8161 cgcgccgaat tctcgatggc accactggtg gacgcaactc ccgaggcagg acgcctcgtt
     8221 ttgagcacca tgcgctcgca cgaccagttc aatacctcga tctactccaa cgatgaccgt
     8281 tatcgtggcg tcaagaacct gcgcaccttg ctcttcatga acgctgtcga tatggaggaa
     8341 aggggcctgg ccgaattcga tcgcatcgac atcacgagct tcgcccgcga tgggagcagc
     8401 cgcagcgtct acggctacct tgccgtgaca tacgacatcc cgcccgggtg cgccgcggga
     8461 tacatgccgg agctcaacgt gttgtgttcg atcggcgatt tcagcgacaa gagcaggcag
     8521 ccgatgatga agcacatccc catcgacgtc accccatcac aagctctata agcgcgcttg
     8581 gggtactgag catgtaagtg attgtcatgt ccatacaagt gagacgtaaa cggtgtgcct
     8641 ccaagcccaa ctggtcacca tgatctccgt gtctgactaa ccttacggtt agattcttgg
     8701 agcctcgagc tcctgacacg cttcttcatg ctcgtttccc cggtccggtc tccggccttg
     8761 ttcataaaca tgggtcctcg ttcatccttg atgatgcatt gttccttgca gcactttccg
     8821 tcgcctcctt cagttgtggc tccactcttc catctggatg cgtagtcgtc tttgtcggtg
     8881 gcataggctc tgctcagttg tggtccctct gctgagagaa cttgggacgc tggatgagca
     8941 ccacaggagc atcgacgatg gaaaaggatt ctgaaccctt cgaacaccaa caagacaccc
     9001 agaataccca tgtaaatgct gacgaaaacc tcgttgtgcc ggtcactgaa gaagagctga
     9061 ctgccacgac acggccggtc gagcgcggcg ccgtccgtat cgagaagcat gtcgtcgagg
     9121 agcaccagac
//
LOCUS       DASYWM010000154         9599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_662332, whole genome shotgun sequence.
ACCESSION   DASYWM010000154 DASYWM010000000
VERSION     DASYWM010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662332"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..1874)
                     /locus_tag="VGR29_06255"
     CDS             complement(447..1874)
                     /locus_tag="VGR29_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEV2073230.1"
                     /translation="MAKLNVVLISTYELGRQPFGLASPAAWLREAGASVTCFDLAVEH
                     LEEEAIAVADMVGFYVPMHTATRIAVSFVHRVKEINPRAHLCFYGLYAPVNEGFLREI
                     GADTILGGEFEEGLIKLAERLSSGVPSLIHQPDPVVSLARQQFLVPDRNGLPELPRYA
                     HLILADGRSRVVGNTEASRGCKNLCRHCPIVPVYGGRFFIVQPEVVLADIEQQVAAGA
                     HHITFGDPDFFNGPTHAMRVVEELHRKFPGLTYDVTIKVEHLLKHAAHLPRLRDTGCL
                     FITSAVEAVDNDILEIFDKRHTREDFIRVVSLFRETGIYLNPTFVTFSPWISLSGYMD
                     LLKLIRELDLVGSVSPIQYAIRLLIPAGSRLLELPAVQDLVGEFDPAAMIYPWAHPDP
                     RVDRLHAAVLKVVSDDQVDQEDRASVFAEVWALTEEACSIPDNQRAEPVSAGRLHLES
                     IPHLSEPWYCCAEPTELQMAPRDHV"
     gene            2688..3383
                     /locus_tag="VGR29_06260"
     CDS             2688..3383
                     /locus_tag="VGR29_06260"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEV2073231.1"
                     /translation="MKVVGVDGCPGGWVAVCWDVTEGAIHPRIHSSLVALLSRYQDAQ
                     AFGVDIPIGLSGTGTRTCDVAARRLLGRGRSSSVFLAPHPDILLAPTYQEAVAWSRSK
                     IQKGVSQQTYAIFAKIAEANATITRELQDRVFEVHPEVSFWALAAEAMVHPKNHLAGY
                     LERKSWLEDGMGVVIPDRTEAFKLARPAKPDDILDATVAAWTARRVADGVAGRLPEEP
                     ETDTKGLRMEIVY"
     gene            3644..4045
                     /locus_tag="VGR29_06265"
     CDS             3644..4045
                     /locus_tag="VGR29_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV2073232.1"
                     /translation="MNQDQAMEYVAANHRGVLATLRRDGRPQLSHVGYMLDDDGLIKI
                     SVTRDRAKTRNAMRDDRVSLSIIGDDWYRYVVIEGRAQIIEDDPLQLLRHVYVGIFGK
                     EHPNWQEFDEAMVRERRVVMAISIDRLYPLD"
     gene            4398..5888
                     /gene="glpK"
                     /locus_tag="VGR29_06270"
     CDS             4398..5888
                     /gene="glpK"
                     /locus_tag="VGR29_06270"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV2073233.1"
                     /translation="MSNEQLILAIDQGTTSSRVVLVNSNGQFVDLAQEEITQIFPQSG
                     WVNQDARHIWEVTLRVVRQVLDRLDGGIERIAAIGITNQRETTVLWNRTTGEPVAPAI
                     VWQSRQTAEIIQDIERRGRAERYQAVTGLVPDAYFSASKIAWLLDRDVGLRARAEAGD
                     ICFGTIDSWLIWKLTNGRTHVTDVSNASRTMLYDIRKGEWSEELLADLHIPKAMLPEV
                     CANSGEIAVSDPSVLGRPIPITGSAGDQQSALFGQACFRPGEAKNTYGTGSFLLMQTG
                     TEAMTSNSRLLTTIAWEVDGETEYALEGSIFVTGSAVQWLRDGLGIIRQASEVEALAL
                     SVEDTGGVAFVPALSGLGAPHWDADARGTIVGISRGTTSAHIARATLEAIAMQSRDVL
                     KAMESDAQIELAELRVDGGAAQNDLLMQMQADLLGVPVVRPRNIETTVLGAAYLAGLG
                     ARVWAGREDVRESWEVDRRFEPTIAESEREARIVLWQDAVDRAKSR"
     gene            6064..6921
                     /locus_tag="VGR29_06275"
     CDS             6064..6921
                     /locus_tag="VGR29_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEV2073234.1"
                     /translation="MVRFEWPEGKTCAAALTFDVDGETIPLVQDPQRGHERLTLISGG
                     AYGPAVGVPRILEVLRSSGIKGTFFIPGFTSELHPGVVHQIVGDGHEVGHHGYLHERP
                     DTLSDDEEAAVLLRGIEVLDRLAGRRPVGYRSPSWELKPGSPGLLKRHQFLYDSSLMG
                     NDIPYRVSTPAGDLLELPVQWILDDYPHFSVNHGGISSPQKVFDVWSTEFAGYHRFNG
                     CYVLTMHPFVIGRPSRIALLERMIEYINQFDGVWWATLEEIATYCASSGMGDIWTPPT
                     LPQASSGKT"
     gene            7002..8483
                     /locus_tag="VGR29_06280"
     CDS             7002..8483
                     /locus_tag="VGR29_06280"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV2073235.1"
                     /translation="MAHEHEVRESSDTEICFLSALELRERYQRRELSPVEVTEAILRR
                     IERFNPRLVAFITVTADLALEQARAAERAYGPGGNPGLLAGIPVSLKDLTPTKGIRTT
                     RGSLLYEDWIPEEDSPFAERVHAAGAVLLGKTNTPEFGWKADTTNRIVGSTHNPWQYG
                     MTAGGSSGGGAAAVAAGMGPLAQGSDGGGSIRIPSSYCGIFGHKPSFGLVPQFPPSAF
                     GDLSHLGPMTRSVRDAALFLDATAGGDPRDRLSWSSGVNYVGSCEGGIAGLRVAWSPD
                     LGYATLDPAVREATERAARRFEDLGCHVEEAGPGLDRPDDVWWKLWISGNAAVHIDDF
                     DRVRDQIDPGRISLIEQGRELTGPELAQQQILRNAYYVGMQQFMERYDLLLTPTMATT
                     AFPAGHNGPTEIAGKPMAYGGDWAPFCSPFNLTGQPAATVPCGFDEAGLPIGLQIVGR
                     WHDDATVLRASAAFESAAPWIQHRPSLDDTFTEASSPGNRSTT"
     gene            8480..9268
                     /locus_tag="VGR29_06285"
     CDS             8480..9268
                     /locus_tag="VGR29_06285"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2073236.1"
                     /translation="MTSSREQAGKLGGNVAIVTGAAQGLGLATARRLVDDGAHVVFAD
                     IQECDPQGFAELGPRAMAMRCDVSDSEGVDLLVATVLQQFGRLDIMVANAGIGRGALI
                     VNMADELFRQVLSVNLEGSFYCCRAAARAMIPARSGVILTVGSTFGRDTPVGNAAYGA
                     SKAGVMAMTAALARELGPHGIRVNCVSPGNMATEMHWSALHRRAGREDVSFETLVNRL
                     RSDIPLGRHGKPEDIAALVSFLVSPDASYISGQTINVDGGYQPR"
     gene            9281..>9599
                     /locus_tag="VGR29_06290"
     CDS             9281..>9599
                     /locus_tag="VGR29_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2073237.1"
                     /translation="MIPIDFSGQVVLVTGGASGLGFAISEAFGRAGATIALNDRSPDR
                     AERAAGELSRAGTACRGFTADVRDAMAVRSMVDRVVTELGPPAVTVVNAGIYPNTPFL
                     ELPE"
BASE COUNT         1866 a         2715 c         3090 g         1928 t
ORIGIN      
        1 gtccccctga atcggggcga agtggtcaaa gagccaggcg tgctcgatgc tgggaatctc
       61 ctcggcttcc tgccagacct tcagcatgtc gtcgtaggtc gtgtgctgcg gcgcggtctt
      121 gatcccgaag ctcaccatag cgttcctcgc ctttcgcttt gttgcatctg gtcaaccatc
      181 attctcacaa actctcgcgt gcgtcagggt agcgcgattc gctcattgat gcactaagag
      241 ccacctgatg ctcgatttac cgccttctcc gtgtttaagc taggcgagcc cgattgtgac
      301 ggtcgacgcg caacgtcaga ccaccggtga tgagccccgt cacagcctgg tcggccaaga
      361 gcatggctga aggagccgcg caccgcaggg aactggtgct agtcttggtt cccgtaacgc
      421 aatcacccat ccctgggcgt ttgccgctag acatgatccc gaggagccat ctgcagctct
      481 gtcggctctg cgcagcaata ccacggctcg ctcaaatgtg gaatggactc aaggtgcagg
      541 cgaccggcag acacaggttc ggctcgctgg ttatcgggaa tgctgcacgc ttcctcggtg
      601 agcgcccata cctcggcgaa gaccgatgcg cgatcttcct ggtcgacctg atcgtcactc
      661 acgaccttga gaacggccgc atgtagccgg tccacccgcg gatcgggatg tgcccatggg
      721 tagatcatgg cagccgggtc gaattcaccg accaggtcct gaacggcagg gagctccagc
      781 agccgcgatc ctgccggaat gagaaggcgt atggcgtact ggatggggga gacgctgccc
      841 acgaggtcaa gctcccggat cagcttgagg aggtccatgt acccactgag cgaaatccac
      901 ggactaaagg tgacgaaggt cggattgagg tatatgccgg tctcgcggaa gagcgagacg
      961 acgcggataa agtcctcacg cgtgtggcgc ttgtcgaaaa tttccaggat gtcgttgtct
     1021 accgcttcca ctgcgctggt gataaagagg cagccggtat ctcgcaacct tggaaggtgg
     1081 gccgcgtgtt tgaggagatg ctcaaccttg atcgtcacat cataggtaag cccgggaaat
     1141 ttccggtgga gttcctccac gacccgcatg gcgtgggtgg gcccgttgaa gaagtcggga
     1201 tccccgaagg tgatgtggtg cgccccagct gcaacctgct gctcgatatc cgcaagcacg
     1261 acctcgggct gcacgataaa gaagcgtcct ccgtaaaccg ggacgattgg acagtgccgg
     1321 cacaggttct tgcacccgcg ggacgcctcg gtattgccga caacacgaga acgtccatct
     1381 gccagtatca ggtgcgcata ccgcggtaat tcagggagcc cgttccggtc agggacgagg
     1441 aactgttgcc gggcgagcga gacgaccgga tcaggttggt gtatcaggga gggcacgcca
     1501 cttgagagcc gctcggccag tttgatgagc ccctcctcga actccccacc gaggattgtg
     1561 tcggctccaa tctcccgcag gaagccttcg ttcaccggtg cgtagagccc gtaaaagcag
     1621 aggtgggctc gcgggttgat ctccttgacg cggtggacaa aggatacggc gatgcgggtg
     1681 gcagtgtgca tggggacata gaaacccacc atgtcggcaa ccgcgatcgc ctcttcctcc
     1741 aggtgttcca cagcgagatc gaagcaggta accgaggcac cagcctcccg cagccatgcc
     1801 gccggcgatg ccaacccgaa cggctggcgg cccaactcgt acgtcgaaat aaggactacg
     1861 ttgagcttcg ccacggtgga gcctccggcg caatgagcct tccctgccaa ccgcggagag
     1921 gtgagcatac gaggagtgct cgatacatta agcgtcgata tcttacggcc agcccctggc
     1981 tcattcgcaa cggtgcaaag agttaggcgc gtttccatgg acgctaggat agacgatcag
     2041 gttcaggtct ggagtggcac atgccgtgga ctcgccgtat cgcggctgcg ggaggcgacg
     2101 atgtgagcga cgttttcagg gagaccgata cggaacgaaa gcatgagagg agttcgacga
     2161 aaagtttccg gcaccggagg ctgctgctgg ctcttgcttc aggatccttg gtccttcaac
     2221 gtgttaccgg aacataacgg agggtgacag gtgctggcaa tgagagccga gtactggttc
     2281 ggcttgccgc acaatttggc gatagtctgt ctcgatatcc ccgaatccgc gccccacaaa
     2341 tacttctgag cctaaggaac cagaggacta tggacgggca aagccgtcga ggagactcgg
     2401 atccgatttc cggtgacggt tgcgctgacg ttcccggtgg cattttctgg cgggagagcc
     2461 accatcttga ctgtgacttg gatgacctcg ggaattcaag cgctaacggc ccgagcatca
     2521 gcactcatat gccaagcgat ccagaggacc attgatgaga ccttgatatt gcctggcatg
     2581 gccagccctt gtccataagg agcatgctgc ctgtgcagcc ttgaacctga caccgtcaac
     2641 gtccgaaact tcggctttcg catatagcgc ggatcaggga gagcagtatg aaggtcgtcg
     2701 gggtggatgg atgtcctgga ggatgggtgg ccgtgtgctg ggatgtgact gaaggcgcga
     2761 tccatccaag gatacacagc tcgctggtgg cactcctctc tcgctatcag gatgctcagg
     2821 cattcggggt tgatatcccc attggcttga gcggcactgg tacacgcacg tgcgatgtgg
     2881 cggcacgtag gctcctgggg cgcggccgca gttccagcgt cttccttgcg ccgcatcccg
     2941 acattctact tgcgccgacg tatcaggaag cggtagcgtg gtcacgctca aaaattcaaa
     3001 agggcgtgtc acagcagacc tacgcaattt ttgccaagat cgccgaggct aatgccacga
     3061 tcacgaggga gctccaagat cgagtatttg aagtgcatcc cgaggtgtct ttctgggcat
     3121 tagccgcaga ggcgatggtt catcccaaga accatctcgc gggataccta gaacgaaagt
     3181 cctggctgga ggacgggatg ggagtagtaa tccctgatcg aacggaggcg ttcaaactcg
     3241 cgcgtcccgc caaaccggac gacattctgg atgcaaccgt ggcagcgtgg acagccaggc
     3301 gcgtggctga tggcgtcgct ggccggttgc ccgaggagcc ggagacggac acaaagggct
     3361 tgcgcatgga gattgtgtac tagatcagcc cggtggctca gggtgccgtt cgtcgtatcc
     3421 ccgttccacc tgagctcagg taccagcatt ctgatcgaga cgaggtccgt ggaatcaagg
     3481 aagcagcacg gtgatctcag tggggaagac gcagcccggc gctcccttac gcgtttgtcg
     3541 ctgtatcgtg gaatcggtcg ccttgaaact tttcgaagtt accgggagca cctgctctcc
     3601 ggcgtggagg agcaccggaa gtagtccggg aatcggagga agtatgaatc aggaccaggc
     3661 gatggagtat gttgccgcga atcatcgggg cgtcctagcg acgctcaggc gggatggacg
     3721 gccgcagttg tcgcacgtcg gatacatgct cgacgatgac ggcttgatca agatctcggt
     3781 gaccagggac cgtgcgaaga cgcggaatgc catgcgcgat gaccgcgttt ccctctcgat
     3841 cattggtgat gactggtacc gctacgtggt gatcgagggc agggcccaga tcatcgagga
     3901 tgatccgttg cagctgctcc gtcacgtgta cgtcggtatc ttcggcaagg agcacccgaa
     3961 ctggcaggag ttcgacgagg caatggttcg ggaacgccgt gtcgtgatgg cgatctcgat
     4021 cgaccggttg taccccctcg actgaccccg tcgtaacagg ggagtggtcc tgacgagcac
     4081 tctgagcttg ctggtctgtc tcatcgctcg gattcctgcg ccagtggaag gtcatgcaaa
     4141 cgagaggacg cttgatgcac tcctgacgtc atgtgtccga aggtcaggca cagaggggac
     4201 atgggcgaat gttccctatc tgtacaacct tctctgggtt gcgtgcacgg catagtcaat
     4261 ggtgactacg gcgacacccg gtgccacgcc agtacgttga tgtcgctgtt ttctaggtcg
     4321 tccttgggga cggctagttt tctctgcggt agcatgaccc gatagtgatg aacgccatgc
     4381 aggagaggaa cgggccggtg agcaacgagc agttgattct cgcgatcgac caggggacga
     4441 cgtccagccg ggtagtgctg gtgaacagca acggtcaatt tgtggatctc gcgcaggagg
     4501 agatcaccca gatcttccct caatccggct gggtcaacca ggatgcccgg catatctggg
     4561 aggttacctt gcgcgtcgtg cgacaggtgc tcgaccggct ggatggaggg atcgagcgga
     4621 ttgccgcgat cggtatcacg aaccagcggg aaacgaccgt tctctggaat cgtacgaccg
     4681 gcgaacctgt tgcaccggcg atcgtttggc agagccggca gacggcggag atcatccagg
     4741 acatcgagcg ccgcgggcgg gccgagcgct atcaggcagt cacgggcctc gtacctgatg
     4801 cctatttctc cgcgtccaag attgcctggt tgctcgatcg ggatgtcggt ctacgtgccc
     4861 gcgccgaggc gggtgatatc tgctttggca cgatcgattc atggctgatc tggaagctga
     4921 ccaacggccg gacccacgtg acggacgtct cgaatgcctc gcgcacgatg ctctacgaca
     4981 tccggaaggg ggagtggagc gaagagctgc tggccgacct gcacatcccg aaggcgatgc
     5041 ttcctgaggt ctgcgcgaac tcaggtgaga tcgccgtttc ggatccatcc gtactcggca
     5101 ggcccatccc gatcaccggc tccgcgggtg atcagcagtc ggcgcttttc gggcaggcgt
     5161 gctttcgccc gggtgaggca aagaatacct atggcaccgg ctccttcctc ctgatgcaga
     5221 cgggcacgga ggcgatgacc tcgaacagcc gcttgctgac cacgatcgcg tgggaagtgg
     5281 atggggagac ggaatacgca ctcgaaggct ccatcttcgt tacaggatct gcggtgcagt
     5341 ggctgcgcga cgggctgggc atcatcaggc aggcgtccga ggtcgaggcc ttggccctct
     5401 ctgtcgagga caccgggggc gtggcctttg tcccggcgct ctcgggtctt ggcgcaccgc
     5461 actgggacgc cgacgcgcgc ggcacgattg ttggcattag ccgcggaaca acctccgctc
     5521 acatcgcgcg ggcgacgctt gaagcaatcg cgatgcagtc gcgggatgtg ttgaaggcga
     5581 tggagtcgga tgcgcagatt gagctggcgg agctccgcgt cgatggtggt gcggcccaga
     5641 acgatctctt gatgcagatg caggcggatc tgctcggtgt gccggtggta cggccaagga
     5701 atatagagac gacggtgctg ggtgcggcct atctcgcggg gttaggagcc agggtctggg
     5761 cgggccggga ggatgtccgc gagagttggg aggttgaccg gcggttcgag ccaacaatcg
     5821 ctgagtcgga gcgtgaagcg cgtatcgtgc tctggcagga tgccgttgac cgcgcgaaga
     5881 gcaggtgagc gctgtggcca gtaccgattt gcacttccgt gataggagac atatccgcaa
     5941 ggatacgcta cggacttgtt gctcgttaac tccagcgtga gcacgtttgt catgatcgaa
     6001 tacgaccggg atgaaatcac gtggggctgg gccggattgt gattgctgtg agaggagaga
     6061 gccgtggtcc ggtttgaatg gccagagggg aaaacgtgtg cggccgcgct gacgtttgat
     6121 gtcgatgggg aaacgatccc gcttgtacag gatccccagc gtggacacga gcggttgacg
     6181 ctcatctccg gcggggcata cggtcccgct gttggtgtgc ctcgtatcct ggaagttctc
     6241 cggtcgtcag gcatcaaggg aacattcttc attccgggat ttacctcgga acttcacccg
     6301 ggcgtcgtcc atcagattgt tggtgatggg catgaggtgg gtcatcacgg atatcttcat
     6361 gagaggccag acaccctctc ggacgacgag gaggcggcgg tccttcttcg cggaattgag
     6421 gtgctcgatc gcctcgcggg ccggcgaccg gttggctacc gctctccctc ttgggagctt
     6481 aagcccggct ctcccgggtt actgaagcgt caccagttcc tgtatgacag cagcctcatg
     6541 ggaaacgata ttccctaccg ggtgtcgacg cccgctggcg atttgttgga actgccagtg
     6601 cagtggattt tggacgacta tccccatttt tccgtcaacc atggcgggat ctcaagcccg
     6661 cagaaagtct tcgatgtctg gtcaactgag ttcgcgggct accatcggtt caacggttgc
     6721 tacgtcctaa ccatgcatcc atttgtcatc ggccggccgt cgcgcattgc cctccttgag
     6781 cggatgattg agtacatcaa ccaattcgat ggtgtgtggt gggccacgct tgaagagatt
     6841 gcgacctatt gtgcttcttc cggaatgggc gatatatgga ccccgccgac gctcccgcaa
     6901 gcatcatcgg gtaaaaccta acgatatggt cagtcacaat tagtggatgg tccgatacct
     6961 gcaacgaacg cggcaaagag caccttcaag aggagaaaaa cgtggcacat gaacacgagg
     7021 tgcgggagtc ctccgacacc gagatctgct ttctctcagc gctcgaattg cgcgaacgat
     7081 atcaacgtcg agagctttcg cctgtcgaag tcaccgaggc aatcctgcgc cggatcgagc
     7141 gattcaatcc ccggctagtg gccttcatca ccgtgaccgc ggacctcgca ctcgagcagg
     7201 cgcgggcagc ggaacgtgcg tacgggcccg gcggcaaccc ggggctcctc gccggtattc
     7261 cggtctcgct caaggatctg acgccgacaa aaggtatccg gacgacccgc ggctcgctac
     7321 tctatgaaga ctggattccg gaagaagact ctccgttcgc tgagcgggtc cacgctgccg
     7381 gtgctgtgct ccttgggaag acgaacactc cggaatttgg ctggaaggcc gataccacca
     7441 accgcatcgt cggctccacg cacaacccct ggcagtacgg gatgacggcg ggcggatcga
     7501 gcggtggggg tgcggcagcc gtggcagccg ggatggggcc gcttgctcag ggaagcgacg
     7561 gcggcgggtc catccggatc ccaagttcct actgtggcat cttcggacac aaaccctcgt
     7621 tcggtctggt tccgcaattt cctccaagtg ccttcggcga tctttcccat ctggggccca
     7681 tgactcgcag cgtccgtgac gccgcgctgt ttctcgatgc caccgcagga ggtgacccgc
     7741 gggatcggct gtcgtggtcg agtggagtca attacgttgg gtcatgcgaa ggaggcattg
     7801 ccgggctccg tgtcgcttgg tcaccggatc tggggtacgc aacccttgat cccgcagtgc
     7861 gcgaagcgac ggagcgtgca gcccgacgct tcgaggatct cggatgccac gtcgaggagg
     7921 caggccctgg actggatcgc ccggacgacg tttggtggaa gctctggatc agtggcaatg
     7981 ccgcagttca catagacgat ttcgatcgtg ttcgagacca gatcgatccg ggacgcattt
     8041 cgctcattga gcaggggaga gagctaactg gaccggagct cgcccaacag cagatcctgc
     8101 ggaacgcgta ctacgtgggt atgcagcagt tcatggaacg ctatgatttg ctgctgaccc
     8161 cgacgatggc gacaacagcc ttccctgcgg gccacaatgg accgaccgag atcgcaggca
     8221 agccgatggc atatggtggt gattgggcgc cgttctgctc gccgttcaac ctgaccgggc
     8281 agccagccgc cacggtgccg tgcggttttg atgaagcggg actgccgatc ggcctgcaga
     8341 tcgtcgggcg gtggcacgac gacgccacgg tcctgcgcgc gtcggcagcg ttcgagtcag
     8401 ctgccccctg gatacagcac cggccctcgc tggacgacac cttcaccgag gcatcatcgc
     8461 ctggtaatcg ctcaacgaca tgacctcttc aagggagcaa gcggggaaac tcggcggaaa
     8521 cgtggcgatt gtgaccggtg ctgcccaagg tctggggctg gcaacagccc ggcgcttggt
     8581 cgacgatggc gctcatgtcg tgttcgcgga catccaggaa tgtgatccac aaggcttcgc
     8641 cgagcttggt ccgcgtgcaa tggccatgcg ttgcgacgtt tccgattcgg aaggtgttga
     8701 cctgctcgtc gccacggttc tccaacagtt cggtcgtctt gacattatgg tcgccaacgc
     8761 gggcattggg agaggcgcgt tgattgtcaa catggcggac gagctgttcc gtcaggttct
     8821 ctcggttaac ctggagggct cgttttattg ctgtcgggcg gcggcccggg caatgatccc
     8881 ggcgcggagt ggcgtcatcc tcacggtagg ctcgaccttc ggtcgcgaca cgccggttgg
     8941 caatgcggcc tatggcgcat caaaggccgg ggtgatggcg atgaccgcgg cgttggcacg
     9001 tgagcttgga ccgcacggta tccgggtcaa ttgtgtgagc cctggaaaca tggcgaccga
     9061 gatgcactgg agcgcacttc atcgccgcgc cggtcgtgag gatgtgtcct ttgagacatt
     9121 ggtgaatcgg cttcggtctg acattccctt gggacggcat ggcaaaccgg aagatattgc
     9181 cgctctcgtg agcttcctcg tttcccccga tgcatcctac atctctggac agacgatcaa
     9241 tgtcgacggt ggctaccagc cacgctgagg aggtgatgtg atgatcccca tcgatttctc
     9301 gggccaggtc gtgctggtga ccggcggagc gtccggtctc ggcttcgcaa tctcagaagc
     9361 atttggtcgg gccggagcga ccatcgccct taacgatcgc tcgccggacc gcgctgagag
     9421 ggccgcaggc gaactttctc gcgcgggaac tgcatgccgc ggatttacgg cggacgtgcg
     9481 cgacgcgatg gccgtgcgca gcatggttga ccgcgtggta actgaacttg ggcccccggc
     9541 ggtgaccgtg gtcaatgccg gaatataccc caacacgccg ttcctcgagc tacctgagg
//
LOCUS       DASYWM010000155        12616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_118354, whole genome shotgun sequence.
ACCESSION   DASYWM010000155 DASYWM010000000
VERSION     DASYWM010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12616
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118354"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="VGR29_06295"
     CDS             <1..1737
                     /locus_tag="VGR29_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073238.1"
                     /translation="LWGRLRVLIPVHLGAVLLAGAVYMLFKDSEPWPAIPWENATPEA
                     TRDYYTALLSHPFVLGMVAVGVVFILGCVIALRWQVSRREDAGSWNEAILGGARPNTV
                     EYGAYHALRDAGGLGIGALVALAIFIGMFTTLFTNLRGIATATYAPNGTLLYWLGQQE
                     VQRGEQPWFYFITEGFQYEWLGIFFATAGTVLVGIRLFKGLLGRDTGPNLLFNVFNAF
                     WFLFLFAVLSWAGEKMPWLIMHFALPGFLVGGALINEIVEGAIGYFRSRRLGAGQLRI
                     PRLGMLGLFGSLIVLALSWYFVAAHLTYGEWNEVSTGGWQRDIPRWAENDWWMLALPP
                     AAALGLIAVGVWLIGARRAAYATLAATIVVFSLFQAHQGFRLAFLEGDTARDTLIYNT
                     TSPDITQLTNDLMEMSEIAYGDESMPVMFDGCSQWPLNWYLRDFPNRQLTSTVPDDPT
                     AQPPVIVGVQSSWDGRCVMPEEMDGYTAQTYVLRWHEPEQAIYREFAIAPEIPAGRSA
                     WRSESQPHDIPAIARSIMSSFEQITGKEGQQRLFRLVMYRELPAGLNPYRFRVYIRND
                     MLPYYNDVRYGE"
     gene            1771..4728
                     /locus_tag="VGR29_06300"
     CDS             1771..4728
                     /locus_tag="VGR29_06300"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEV2073239.1"
                     /translation="MRAERAAGSVDSPESPHDTAEQVTSEPPDPVLHRRLMFPLGGGV
                     TLLILGAFVAALLLRLVRLDAYVLGDKEASWAYDAWSLFRGRPLPGTEQIPDTAPLLL
                     VTEAFTFFLFGVTDATARFGPALFGIGIIVLLLALRPLVPRFAVAGMVVLAALSPTLV
                     YASRTIDPAIGVAFSTLLLVVSLLRSGHAVSLGSRSGWIVLAGAGAAGALGGGPEGVS
                     ALIAAFAGLFLAAVAEPGNGGAVREGFQAIGRSPRNQVLFVLAFVLSLAVLYTRMFSD
                     VGALEGIVTTFADWGRMIASRSTRIPQSFFFYAVLLYEIFALVFAIVALAATPGDERR
                     RDGEQNRISPVLFGGWFAVSLLLFSFASGREAEQAVLVVLPLVVMGGMGLGHVLEKVR
                     WSGFWTSRAGLLPLVLLVFFVGVIAIIMVIARSNDPGAANPSDWPPVLQIFFILAAVV
                     IPAAYLIWTRSVDEPNTGELGNAALLVLALVLALFTIRSATALAFYRADDGTELLAPN
                     VPTQGIRAFVDQVYRLSRDLSVENLSNVDNTGSLGLQLAIGPDVEWPFLWYFRDFPNV
                     RVEGPAGWTEDDDVVVAASPEGMDTAGFVVQQKAFENRTANAYVDLSANTVFGNIFSP
                     DEWYDGFRFLFFREMESEQPVETVAVGYAFRISNQINPNLGPFDLFTQNTPGPGGGLG
                     QLSVPTGIAVSPDGQVIYVVNAGNQRIERYARDGSFLGIWDTATDPSLGLAFQNGQGA
                     SDIETGDDGLIYVTDTWNHIVVVLDQEGNVVRQLGQRGVLTDIGDGGDPASEPGLFFG
                     PRGIAVLNDEIYVSDTGNERIQVFSKDGTFLRAFGGFGTGQGELQEPTDIAFGPDGNL
                     YVADSGNGRLVVYTPEGEVLDEIPVESWTELVGADRVNYVAFNQDGILYLTAPARGVL
                     EAYDGEEFVPIEGNELVRPVGVAVAPDEMVLVTDGAESTVIQIVPDLPEGFGAEASPV
                     ASPQASPLASPAS"
     gene            complement(4809..5108)
                     /locus_tag="VGR29_06305"
     CDS             complement(4809..5108)
                     /locus_tag="VGR29_06305"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEV2073240.1"
                     /translation="MTGTIGKARVTLYFEDTFSIKEKRGEVKRVTSRIRNRFNVAVAE
                     IDDLNDMRVATLGMTVLSTSTPHADQMLETIIAAIEDLLDISVLGDIETEMIPFG"
     gene            complement(5105..5590)
                     /locus_tag="VGR29_06310"
     CDS             complement(5105..5590)
                     /locus_tag="VGR29_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073241.1"
                     /translation="MDDHSLSHRETSFEIEHGHDAYTNEELLEQSQFNAQALLLAAVA
                     ALDGDDAAIDTWTKGVGEVFIRGWDQQRTWEASEILDALLTNYRSFGAQVIEVDLEAE
                     HPAATIADLPDLGLAEDLQVPEEKADAIFQIGGHIVAALGHTLVWSRAADSGDVTLTV
                     T"
     gene            complement(5611..6720)
                     /locus_tag="VGR29_06315"
     CDS             complement(5611..6720)
                     /locus_tag="VGR29_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV2073242.1"
                     /translation="MQHIDTSVYRSRITRAQEALRERSIAALLLGPSADLTYLTGFDA
                     HHSERMNLLILPADAEPSLVIPTLEASLVGDAADLVTLHDWADQDRPAELAASILGNV
                     AGKTLAVGNQLWSAFLLRLQAELRGATWVEGDSVLRELRMIKEPVEISYLAEAASLTD
                     LAWEEFIRSGQISGLTELQALQRLLDISTAQGLSKASGIVGSGPNSASPHHSGDDRVI
                     QSGDAVVFDWGGRINGYFSDVTRSVHVGEPGEEYRRVYEIVKRANQATFETIKPGVPL
                     QELDRTARTLITGEGYGDAFLHRVGHGLGMEIHEEPYLVEGNDLPLASGMVFSDEPGI
                     YLSGRFGVRIEDTVVCTEEGGRYLNNATRELVVMK"
     gene            6939..7886
                     /locus_tag="VGR29_06320"
     CDS             6939..7886
                     /locus_tag="VGR29_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073243.1"
                     /translation="MVAGSLAFLPAETYPPSVAAQGEGKKEKNGEDKKNNQGDNKSKD
                     KNKKEDKDKDKGPKKDNDGEVVAAVAYAVDVQCEYDEDAVSTACSFTGVAPSDAKDVS
                     HVDLPQEEVCAEVTDGTFEYVDPDPNTGVTGYKSRGSEGSFTLVLDGEVTAAGTATYW
                     FKTGDGVFPATGPGLSCEQASAEFVVQTPEETAEETAVVTDGTGALLVDIYSCTAVPE
                     DATSFDWFGKCDPEGGVHEFTLAPAGAVAGEPLTQVSEASGNTSFNTLDPGLYSLELV
                     DAKWCHAVSDNVDAKGQVAIEAGERTTVWGFICGDDGVK"
     gene            8083..10383
                     /gene="serA"
                     /locus_tag="VGR29_06325"
     CDS             8083..10383
                     /gene="serA"
                     /locus_tag="VGR29_06325"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEV2073244.1"
                     /translation="MHQRSDSLRFQPQETPPQLRVAVDLDGVLTEYPAPLADAANRQF
                     GLSLPVRAFVDSAGLNVPLHVREWVYDREGPASRLYPAQGAQEFVRQLVGLVGEGNVM
                     IITARPENSATTTVNWLKHHGFMSCNVIFGDDKMTIARRQGCGYAIEDSERHAYNYAA
                     GGLFCFLIGTDKTAAEMEEENVIPVGEFADILEYLGERLDSTGRFRHLATLPPVTADG
                     SVDRPRIVIADAVHPVARQEFEAHGDVVDVDGMDVPALKDAVRDADALVVRSETLVTA
                     EILGAAPRLKVVARAGVGVDNIDIPAATRAGVIVLNAPGTNAVSAGEHTIALLLALTR
                     QIPFANSTTHAGEWKRKQVKPVDLRGRTVGIVGLGRVGTIVARRLKAFEMTVIAHDPY
                     IPASRFTEIGVEQVTLEEIFLRSDVVTFHCPAGDDTYHLLNAQTLAKMKPGSIVLNAA
                     RGEVVDQQALADALRSGHIAAAGVDVFPSEPCTDSPLFGLENVVVTPHTGGSSIEALE
                     AVGRVIGATTMAALRGEAVPNAVNLPPASLEAPALKLLTSVAGAAGHLMSVLMPEIPA
                     GISLEARGLISNDITEHVMNAALSESLRRWLGRRVTPVNARMVADELGLSVATCADDG
                     DATSLPQFRFEVHGETSHSVVITWDRTIAGIVEVDRFSLLRPLEGYVLITHHRDQPGV
                     IGRLGTILARHEVNIAGMQVSRDMPRGEAMMVTNVDEQIPQVALDEIKAVGGVDEAFV
                     VSLPSLTETQDPITLASITSPVMNAV"
     gene            10666..11301
                     /locus_tag="VGR29_06330"
     CDS             10666..11301
                     /locus_tag="VGR29_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073245.1"
                     /translation="MKPSKFLLMIVTLCTLLLFAVSASAQAVDLGNDGTDMSCEDFPE
                     FNAANGYFLHDGGSAERNVDNLDPDGDGIPCNEMPDNADNDGASVGPDPGNDGVDMNC
                     DDFPERNAVEGYFAQDGGSDGRNVDGLDPDGDGVPCNEENYDAPAEEDNDPEDETPAD
                     DSANPGPVTSLPSTGSGPDTREGAAGLYAMVATLIAIAGSFAFSVLRRAVR"
     gene            complement(11462..>12616)
                     /locus_tag="VGR29_06335"
     CDS             complement(11462..>12616)
                     /locus_tag="VGR29_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV2073246.1"
                     /translation="EEDIYAGFWGRTTRLVVGRPVLSMVIAVVLLVALALPVLDLRTG
                     FTGTAQMPPGEITDAYRVLERDFSAGVLAPVEFVFQGEHTKEADAALEVLRAELEATG
                     EFAPLGQPVLWDEEQQLAVMERTMIHPPSSEEAYELLRQVRSEIIPATIGDVDGLDSW
                     VTGPSAFEIDMLDMLENQTPLVFAFVLGLSFVLLTPAFRSLIVPLQAIVFNLLSVGAT
                     WGIMVMVFSKGVLRDFFGFHASLVIESWIPILLFCILFGLSMDYHVFILSRIREHYDI
                     THDNRESVAVGLRSTGKTITGAALIMVVVFGAFATGSMLALQQLGFGLAVAVLLDATI
                     VRSVLVPSVMTLLGDRNWWLPGWLTWLPDLRIEGEPQAPTRAQHSPAAGD"
BASE COUNT         2402 a         3740 c         3827 g         2647 t
ORIGIN      
        1 ctgtggggcc gcctccgggt cctgatcccg gtccatttgg gtgcggtgct gctcgcgggc
       61 gctgtctata tgcttttcaa ggacagtgag ccgtggcctg ctataccgtg ggagaatgcc
      121 acgccggagg cgactcgcga ttactatacc gccctcctct cccatccttt tgtgctgggg
      181 atggtggccg tgggcgtcgt cttcatcctt ggatgcgtca ttgccctgag atggcaagtt
      241 tcccgacgtg aagatgcggg tagctggaac gaggcgatcc tgggcggagc acggccgaac
      301 acggtggaat acggcgccta tcacgccctc agggatgctg gtgggctcgg tatcggggcg
      361 ctggttgcgt tagctatttt catcgggatg ttcactacgc tcttcaccaa tctgcgcggc
      421 atcgccacgg caacatatgc gccgaacggc acgctgctct actggttggg gcagcaggaa
      481 gtgcagcgcg gcgagcagcc ctggttctat ttcatcaccg agggcttcca gtacgagtgg
      541 cttggtatct tcttcgcgac ggctggaacc gttttggtcg gcattcgcct tttcaaggga
      601 ctccttggca gggataccgg ccccaacctg cttttcaacg tcttcaacgc tttctggttc
      661 ctcttccttt tcgctgtcct ttcatgggcg ggtgagaaaa tgccctggtt aatcatgcac
      721 ttcgcgctgc cagggttcct ggtcggtggg gcactgatca acgagatcgt ggaaggggca
      781 atcggttact ttcgatcgcg tcggcttggc gccgggcagc tgcggatacc gcgtcttggc
      841 atgctcgggc tcttcggctc actcattgtg ctggcactgt cctggtattt tgttgccgcc
      901 catctcacct atggcgaatg gaacgaggtg tccaccggtg gttggcagcg ggatattccg
      961 cgctgggcgg aaaacgattg gtggatgctt gccctgcctc cagcggccgc gctgggtctc
     1021 atcgccgttg gggtgtggtt gattggcgca cgccgtgctg cctacgcgac gctggcggcg
     1081 acgatcgttg tcttcagcct gttccaggca catcagggct tcaggctggc atttctcgag
     1141 ggtgacaccg cccgggacac gctcatctac aacaccacat cacctgatat tactcagctc
     1201 accaatgacc tgatggagat gtcggagatc gcctatggcg acgagagtat gccggtgatg
     1261 ttcgacggtt gttcccagtg gccgctgaac tggtacttgc gtgactttcc gaaccggcag
     1321 ttgaccagca ccgtccctga cgatcccact gcccagcctc ccgtcatcgt cggagtccaa
     1381 tcctcatggg acggccggtg cgtcatgcct gaggagatgg atgggtacac ggcgcagacg
     1441 tatgtattgc gatggcatga gccggagcag gcgatctacc gggagttcgc gattgcgccg
     1501 gagatcccgg ctggccgctc ggcgtggcgg tccgagtctc agccgcacga catcccggcg
     1561 atagccaggt ccatcatgag cagctttgag cagatcaccg ggaaggaggg gcaacaacgg
     1621 ctcttccgtc tcgtgatgta ccgcgagctg cccgccgggc tcaaccccta tcgcttccgg
     1681 gtctatatcc gaaacgatat gttgccgtac tacaacgacg tgcggtatgg cgagtaacgt
     1741 tgcagcacga ggtgcatgag gagactcgaa ttgagagcag agcgcgcggc cggatcggtg
     1801 gatagccccg aatctcctca cgacacggcg gaacaggtta cgagcgagcc accggatcca
     1861 gtgctgcacc gccgcctgat gtttcccctt ggtggcgggg ttacgctact catattgggc
     1921 gcctttgtcg ctgccctgct gttgcgcctt gtgagactcg acgcctatgt gctgggcgac
     1981 aaggaagcca gctgggccta tgatgcatgg tcgctcttcc gggggcggcc actgcccggg
     2041 acagaacaga ttccggacac tgcgccgctc ttgctggtca ccgaagcgtt cacgtttttc
     2101 cttttcggtg tcactgatgc aaccgcgcga ttcggccctg ctctcttcgg gatcggcatc
     2161 atcgtcctcc tcttggcgct gcgtccgctt gtgccccgct ttgcagtcgc cggcatggtg
     2221 gtgctcgccg ccctctcacc tacccttgtt tacgcttccc gcaccatcga tccggcaatt
     2281 ggtgtggcgt tcagcacctt gcttctcgtc gtgtccctcc tgcgctcagg gcatgcggtc
     2341 tctttgggca gcagaagcgg ctggatcgtg ctcgcagggg ccggggctgc aggcgccctt
     2401 ggtggcgggc cggagggtgt tagtgccctg atcgcagcgt tcgcggggct gttcctggca
     2461 gcggtggcag aaccgggcaa cggcggggcc gttcgtgagg gatttcaggc cattggccgg
     2521 tccccgagaa accaggtgtt gttcgtcctg gcgttcgtcc tctccttggc agtgctgtat
     2581 acgcggatgt tttcagacgt cggcgccctg gaggggatcg ttaccacctt cgcggattgg
     2641 ggccggatga tcgcgtcgcg ttcgacacga attccgcagt ccttcttttt ctacgccgta
     2701 cttctctacg agatattcgc cttggtcttt gccatcgtcg cccttgcggc aacaccaggg
     2761 gatgagcgcc gccgggacgg cgagcagaat cggataagcc ctgttctctt cggtgggtgg
     2821 ttcgcggtct cactgctgct ctttagcttc gcttccgggc gtgaagcaga gcaggctgta
     2881 ttggtcgtgc tgccactcgt cgtgatgggt ggcatgggcc tcggccacgt attggagaag
     2941 gtcaggtgga gcgggttctg gacctcacgc gcgggtctcc tgcccctggt gctgctcgtt
     3001 ttctttgtag gcgtcatcgc cattatcatg gtcatcgccc gttccaacga ccctggggcc
     3061 gccaacccaa gcgactggcc gccagtcttg cagattttct tcatccttgc cgccgtcgtc
     3121 attccagccg cctacctcat ctggaccagg agcgttgatg agccaaacac cggggagctg
     3181 ggcaacgctg ccttgttggt gctggcactg gtgctggccc tctttacgat acggagcgct
     3241 accgctctcg ccttctatcg tgcggatgac ggcactgaac tactcgcgcc gaatgtgccg
     3301 actcagggga tccgtgcctt cgtggatcag gtctatcgcc tctcgcgcga cctgagcgtt
     3361 gagaacctct cgaacgtgga taacacgggt agcctgggtc tccagcttgc aatcgggccg
     3421 gacgttgagt ggcccttcct ctggtatttc cgggacttcc cgaacgttcg ggtggagggc
     3481 ccggcgggct ggacggaaga tgacgacgtt gtggttgcag cgtcgccaga aggtatggac
     3541 acggccggtt tcgtggtaca gcaaaaagca ttcgagaacc gcaccgcgaa cgcgtacgtt
     3601 gatctgagcg cgaataccgt tttcgggaac atcttttctc cggacgaatg gtatgacggc
     3661 tttcgctttc tctttttcag ggaaatggaa tctgaacagc cggtggagac cgtcgcggtt
     3721 ggctatgctt tccggataag caaccagatc aaccctaatc tcggaccctt cgatctcttt
     3781 acccagaata cccccggtcc gggcggtggc ttgggacaac tctccgtccc gaccggaatt
     3841 gcagtctctc ctgacgggca ggtgatctac gtcgtgaatg cgggcaacca gcgcatcgag
     3901 cgatatgccc gggacggttc cttcctcggg atctgggaca ccgcgaccga tccgagcctt
     3961 gggttggcat tccagaatgg ccagggagcc tccgatatcg agacgggtga cgatgggctg
     4021 atctacgtga ccgatacctg gaaccatatc gtcgtcgtcc tggatcagga aggaaatgtc
     4081 gtccgccaac ttggacagcg gggcgtcctg accgatatcg gagatggtgg ggatccggcc
     4141 tccgaaccag ggttgttctt cggcccacgc ggcatcgcgg tcctgaacga cgagatctac
     4201 gtctccgaca cagggaatga gcgcattcag gtctttagca aggatggcac cttcctccgc
     4261 gccttcggtg ggtttgggac ggggcaagga gagttgcagg agccgacaga cattgcgttc
     4321 ggtcccgacg gaaacctcta cgtcgctgat tccggcaacg gtcgtctcgt cgtttacacg
     4381 ccagagggcg aggtattgga cgagatcccg gtggagtcgt ggacggaact ggttggcgct
     4441 gatcgcgtca attacgtcgc tttcaatcag gacggcatac tgtacctgac tgcacctgca
     4501 agaggagtgc tggaagccta cgacggcgag gaattcgttc ccatcgaagg caatgagctt
     4561 gtgcggccag tgggtgtcgc ggttgctccg gacgagatgg tgctcgtcac ggacggcgcc
     4621 gagagtaccg ttatccagat cgttcccgat ctcccggagg ggttcggcgc ggaagcttcg
     4681 cccgtggcga gcccgcaagc atcgcccctt gcatcacccg ccagctgacg aggaattcca
     4741 accggagagc cggagtatat aaacatctgg ctctccctcg cagcgggtcc cgaaaccggg
     4801 ctatttgctc aaccgaacgg gatcatctct gtctcgatgt cgccgagaac gctgatgtcg
     4861 agcaggtcct caatggcggc gataatcgtt tccagcatct ggtccgcgtg gggtgttgaa
     4921 gtgctgagga ccgtcatccc cagcgtcgca actcgcatat cgttcaggtc gtcgatctcc
     4981 gcgacagcga cattaaacct gtttcgaatt cggctcgtga cccgctttac ttcccctcgc
     5041 ttctccttga tggaaaaggt gtcttcgaag tagagcgtca ccctcgcctt gccgatcgtt
     5101 ccagtcatgt cacggtgagc gtcacgtcgc cggaatccgc cgcacgcgac cagacgagcg
     5161 tgtgaccgag ggcggcgaca atatgccccc cgatttggaa gatggcgtcc gccttctcct
     5221 cgggaacctg cagatcctcg gccaatccga gatccggcaa gtcagctatc gtggcggccg
     5281 ggtgctccgc ctcgagatcg acttcgatca cctgcgctcc gaacgagcgg tagttcgtga
     5341 gcagggcgtc aaggatctct gatgcctccc aggttcgctg ctggtcccac ccgcgaatga
     5401 agacctcgcc gacacccttt gtccatgtgt cgatcgcggc gtcatctccg tccagtgcag
     5461 cgaccgcggc aagcagcagg gcttgggcgt tgaactgcga ttgctcgagc aactcctcat
     5521 tggtgtaggc gtcgtggcca tgctcaatct cgaatgatgt ctcgcggtgt gacagcgaat
     5581 gatcgtccat agagcctccg ttcagtccgg ctatttcatc accaccagct cgcgagtggc
     5641 gttgttcagg tatcgcccac cctcttcggt acagacaaca gtgtcctcga tgcggacacc
     5701 aaaccggcca cttaggtata tcccgggctc gtcactgaac accatcccgg aggcaagcgg
     5761 gaggtcgttg ccctcgacaa gatatggctc ttcgtggatc tccatcccaa gtccatgccc
     5821 cacacgatgg aggaatgcgt ctccatatcc ttcgccggtg atcaaggtcc tcgcagtgcg
     5881 gtcgagttcc tgtaacggta ctcccggttt gatggtctca aacgtcgcct ggttcgcccg
     5941 cttaactatc tcgtagacgc ggcggtactc ctcgcccggc tctcccacat ggaccgatcg
     6001 ggtgacatca gagaaatatc cattgatccg tccgccccag tcgaagacga ccgcatcccc
     6061 gctctggata acccggtcgt ctccactatg atgcggtgac gcggaattcg gaccacttcc
     6121 aactatgccg gaggccttcg aaagcccttg cgccgtgctg atatcaagga gacgctgcag
     6181 ggcctggagc tccgtgagcc ccgatatctg gcccgaccgg atgaactctt cccaggcaag
     6241 gtcggtgagg cttgctgcct cggcgaggta gctaatctcc accggctcct tgatcatgcg
     6301 tagctcccgg aggacggagt cgccctcgac ccatgtggca cccctgagtt ctgcctggag
     6361 ccggagcagg aaggcgctcc aaagctgatt gcccactgca agcgttttgc cggcgacatt
     6421 tccgagaatg cttgctgcga gctcagccgg gcgatcctga tccgcccaat cgtggagcgt
     6481 caccagatct gcggcatcac ccacgagcga cgcttcaagt gttgggatga cgagcgatgg
     6541 ctcagcatca gccggaagga tcaggagatt catacgctcg ctgtggtgcg catcgaatcc
     6601 ggtcaggtac gtcaagtcgg ccgacggtcc gagcagcagc gcggcaatgc tgcgctctct
     6661 taatgcctct tgtgccctgg tgatgcggct acgataaacg gacgtatcga tgtgttgcat
     6721 gatctgctcc caagtatgcg tatgcgtgtc acatgtagcc gttatcgcag agtgtgcacg
     6781 atccgtcgag acggtcgcac ggggtgtacg agcgacagga gcggaatggt tgctggcatg
     6841 tcggctgcgc aataatgctc cctaacttga taacccttcc cggagaggac tagcgaatgc
     6901 tcactcttat cgcctggcga cttgccgcaa tcctgttgat ggtggccggt tcgctggctt
     6961 tccttccagc cgagacttac ccaccaagcg tagctgcaca gggtgaaggg aaaaaggaga
     7021 agaacggcga ggacaagaag aacaatcaag gtgacaacaa gagcaaggat aaaaataaga
     7081 aagaggataa agacaaagac aagggcccca agaaagataa cgacggcgag gtggtggcag
     7141 ccgttgcgta tgccgtcgac gtgcagtgcg aatacgatga agacgcagta agcaccgcct
     7201 gctcctttac gggtgtcgct ccttctgatg ccaaggacgt cagtcacgtc gatctccctc
     7261 aagaggaggt atgtgctgag gtcacggacg ggaccttcga gtacgtcgat ccggatccca
     7321 acacaggcgt caccggatac aaatccaggg gaagtgaagg ctcattcaca ttggttctcg
     7381 acggggaagt aaccgccgcg ggaacagcga cctattggtt caagaccggt gatggggtgt
     7441 tcccggccac aggtcctggc cttagttgtg agcaagcctc cgccgaattt gtcgttcaga
     7501 cgccggagga gacggctgaa gaaaccgctg tcgtcaccga cggcaccggt gctctgctcg
     7561 ttgacatcta ttcatgcacc gccgttccgg aggacgccac gagctttgac tggtttggca
     7621 aatgcgatcc agagggtgga gttcatgaat ttaccctcgc cccagccggc gcagtggccg
     7681 gtgagcctct cacacaggtg tcggaggcat ctggcaatac ctcgttcaat acacttgacc
     7741 cggggttgta tagcctggaa ctggtagacg cgaaatggtg ccatgcagtg tcagacaatg
     7801 tcgacgccaa gggccaggtt gccatcgaag ctggggagcg cacaaccgtg tggggattca
     7861 tctgtgggga tgatggcgta aagtaagagg cagggtgctc cactggcgta acacgtagcg
     7921 caactcctgc tgcgatgcag ccgtggatac ccgcaatgtg ggcatgccta caaatacact
     7981 gacattgcgg caccatacgt cctacactgc aggtgcaccg gcaatggcgc cggcctaccc
     8041 attgctggtt gtctctaaac gcaggcaagg tgtcattccg tcatgcatca acgctcagat
     8101 tcgcttcgct tccagcccca ggagacgcct cctcaactcc gtgtcgccgt tgatctcgat
     8161 ggcgtgctga ccgaatatcc cgcgcctctg gccgatgccg ccaacaggca attcgggctc
     8221 tcgcttccgg tgcgcgcctt cgttgactcc gcagggctca acgtcccgtt gcacgtccgc
     8281 gaatgggttt acgaccggga aggccccgcc tcacgcctct atcctgccca gggggcacaa
     8341 gagtttgtcc ggcaactcgt cggcctcgtt ggcgaaggca acgtgatgat catcacggca
     8401 cgcccggaga actccgcgac gacgacggtc aactggctga agcatcatgg attcatgagc
     8461 tgtaacgtca tcttcgggga tgacaagatg accattgccc gacgtcaggg atgcgggtat
     8521 gccatcgaag atagtgagcg gcatgcttat aactatgcag ccggagggct cttctgcttc
     8581 ctaattggca cagacaagac cgcggctgag atggaagagg agaacgtcat cccggttggc
     8641 gaattcgcgg acattctcga atatcttgga gagcgtctcg acagcactgg ccgcttccgc
     8701 catcttgcaa ctttgccgcc tgtcacagca gacggttctg tagatcgtcc aagaattgtc
     8761 attgcggacg cggttcaccc tgttgctaga caggagttcg aagcacatgg cgatgttgtt
     8821 gatgtcgacg ggatggacgt cccggcgctg aaggatgctg tccgcgatgc ggatgcgctc
     8881 gtcgttcgca gcgagacgct ggtaacggcg gaaatcctcg gggcagcacc gcggttgaag
     8941 gtggtcgcgc gtgcgggtgt cggagtcgat aacatcgata ttccggcagc cacgcgggcc
     9001 ggggtgatcg tcctcaacgc tccgggcacg aacgccgtat ccgccggtga gcacacgatc
     9061 gcgttactgc ttgcgctcac tcgccaaatt cccttcgcca atagcacgac gcacgctggt
     9121 gagtggaagc gcaagcaggt aaagcccgtc gacctgcggg gccgcacggt cggtattgtc
     9181 ggtttgggcc gtgtgggtac tatcgtcgcg aggcgtctca aggcgttcga gatgacggta
     9241 atcgcgcatg atccctacat tccggcatcc cgattcaccg agattggggt tgagcaagtg
     9301 acattggagg agatcttctt gcgcagtgat gtcgtcacct tccactgtcc tgcgggcgac
     9361 gatacctacc acctgctcaa cgcacagacc ttggcgaaga tgaagccggg ctcgatcgtg
     9421 ctcaacgccg cgcgtggcga ggtcgtggat cagcaagcgc tcgccgatgc tctccggagt
     9481 ggccacatcg ccgccgctgg cgtcgatgtc tttccatcgg aaccatgcac cgacagccct
     9541 ctcttcggcc ttgagaacgt tgttgtaacg ccgcacaccg gcggctcaag cattgaggcg
     9601 ctggaagcag tgggtcgggt tatcggcgcg acgaccatgg ccgccctccg tggtgaagcc
     9661 gtacccaacg cggtaaacct gccgccggca tcactggagg cacctgccct caagctcctc
     9721 acctcggtcg ccggtgcagc gggacacctg atgtcggtgc ttatgccgga gattccagcc
     9781 ggcatctcgc tcgaggcgcg cggactgatc tcaaacgaca tcactgaaca tgtgatgaac
     9841 gctgccctga gtgagtcgct gaggcgctgg cttggcaggc gcgtgacgcc cgtgaacgct
     9901 cgtatggtcg cggatgagct cggcctgtcc gtcgcgacgt gtgccgacga cggtgatgcg
     9961 acctcactgc cgcagttccg gtttgaggtc catggcgaga cctcacactc agtcgtgatc
    10021 acctgggacc gtacgattgc cggtatcgtc gaggtcgacc gcttctccct gcttcgcccg
    10081 ctggagggat acgtgctgat cacccatcac cgcgatcagc cgggcgttat cggcaggctg
    10141 ggaaccatcc tcgcgcggca tgaggtcaac atcgccggga tgcaggtcag ccgcgatatg
    10201 ccacgcggtg aggccatgat ggtgaccaat gtcgacgagc agatcccgca ggtagccctg
    10261 gacgagatca aggcggttgg cggagtcgac gaggccttcg tggtttccct gccgagcctt
    10321 accgagacgc aagacccaat cactcttgct tccatcacct cccccgttat gaacgccgtc
    10381 taaaccctat ttccggaaac gaacgacacg agggcggacc gatcggcccg ccctcatttt
    10441 gtgtctgaat gcacctcacc cccatcactg tcctgttttc cggcgagctt cggaaactgt
    10501 tggacggcac ttagacagca cggcagcatc ggtgccgtga ttaggtaagt ccgattcggt
    10561 agccctaccg atgcccagcc tgtgttcacc tcgcagaatg gaatatgtcg gcaattggtc
    10621 gacatccaat ccgatacgaa gcgactattg aggagagcac ctaccgtgaa acccagcaag
    10681 tttctgttga tgattgtcac actctgtacg ctattacttt tcgccgtttc tgcaagtgct
    10741 caggctgttg acctcggcaa cgatggcacc gacatgagct gtgaggactt tccggagttc
    10801 aacgctgcga atggttactt cctccatgat ggcggaagtg ccgagcggaa cgtggataac
    10861 ctggatcctg atggcgacgg cattccctgt aacgagatgc ccgataacgc tgataatgac
    10921 ggcgcaagcg tcggtcctga ccctggaaac gacggcgtgg acatgaactg cgacgatttc
    10981 cccgagcgca atgcagtgga ggggtacttc gcgcaggacg gtggcagcga tggacgcaat
    11041 gtcgacggcc ttgatccaga cggggacggc gtcccgtgta acgaagagaa ctacgacgct
    11101 ccagctgagg aagacaacga ccccgaagac gagaccccgg ccgatgacag cgcaaaccca
    11161 ggtccggtca cctcacttcc ctccactggc tcgggccctg ataccagaga aggcgctgca
    11221 ggattgtatg cgatggttgc gaccctgatt gctattgccg ggagttttgc cttcagcgtc
    11281 ttgcggcggg cagttcggta acggacgctt acaccggtcc cctattcacc ttggacaacg
    11341 cccgcgagtt cgctcgcggg cgttgctatc tgcggtgagg ccaggagtag cgaggtccgg
    11401 ccaggcaatg actggctgca ttcgtttcca tcaacaggac cgcctgggcg aaagtgcctt
    11461 actaatcgcc tgccgccggc gagtgttgcg ctctagtggg agcctgtggt tcgccctcga
    11521 tgcggagatc gggcagccac gtgagccatc ccggcagcca ccagttcctg tcgcccaaga
    11581 gtgtcatgac ggagggaacg agcaccgagc gcacgatcgt ggcatcgagc aggacagcga
    11641 cggcgagtcc aaacccgagc tgctgcagtg cgagcatgga gccggtcgcg aacgcgccga
    11701 agacgacgac catgatgagt gccgcgccgg tgatggtctt gccagtgctg cggaggccga
    11761 cagcaaccga ctccctgttg tcatgcgtga tatcgtaatg ctcgcgaata cggctcagga
    11821 tgaaaacgtg gtagtccata cttaggccga acaggatgca gaagagaaga atcgggatcc
    11881 aggattcgat caccaggctg gcatggaagc caaagaaatc tcgcagcact cccttcgaaa
    11941 agaccatcac catgatgccc caggtggcgc ccaccgagag caagttgaag acaatcgcct
    12001 ggagtggcac gatcagcgag cgaaacgccg gtgtcagcag cacgaagctc agtccgagca
    12061 cgaaagcaaa gactagcggg gtctggtttt caagcatatc gagcatgtct atctcgaacg
    12121 cgcttggacc agtgacccag gaatcgagac catcgacatc cccgatcgtc gccgggatga
    12181 tttcgctacg cacctggcgt aggagctcgt aggcttcctc gctggagggc ggatggatca
    12241 tggtcctctc catgacggcc aactgctgct cttcatccca taggactggt tggcccaagg
    12301 gggcaaactc tccggtggct tccagttctg cccggagcac ctccagagcc gcatccgcct
    12361 ccttggtgtg ttcgccctga aacacaaact ctacgggcgc cagcacgcca gcgctgaagt
    12421 cccgctccag caccctgtag gcatctgtta tctcgcctgg aggcatctga gcggtaccgg
    12481 taaatccagt gcggaggtcg agcaccggaa gggccagggc caccaagagc acaaccgcaa
    12541 ttaccatcga caatactggt cggccgacca caagtcgggt tgttcttccc cagaacccgg
    12601 cgtagatgtc ttcctc
//
LOCUS       DASYWM010000156         6533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_606517, whole genome shotgun sequence.
ACCESSION   DASYWM010000156 DASYWM010000000
VERSION     DASYWM010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606517"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..640
                     /locus_tag="VGR29_06340"
     CDS             392..640
                     /locus_tag="VGR29_06340"
                     /inference="COORDINATES: protein motif:HMM:NF041742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGxxGxxG family protein"
                     /protein_id="HEV2073247.1"
                     /translation="MAGLRAAALALLLGAFMTGGVVAQDTPAALAQVDEAAEEVEDEG
                     FDDWGLLGLLGLAGLAGLMKRNPEPVVHTELDRDRPRV"
     gene            complement(991..2880)
                     /locus_tag="VGR29_06345"
     CDS             complement(991..2880)
                     /locus_tag="VGR29_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2073248.1"
                     /translation="MSSDFRNGPYSALFTSLKQGELSRREFIERATALGMGLSVAMYC
                     ANTVTAQESSPAASPESERVAGVGTIPAAGTENQERGAGGELKVLQWQAPSQLNGMVA
                     TGDKDNLAALFVSESLMVRLPDGALAPQLVTEVPTVDNGLLAEDLKSVTFNLKTGVLW
                     SDGEPFTAEDVRFTWEWALDDANGAVLQNVYELIKDIEVVDELTAKVTFANSNPSWAD
                     AFTGMGASIVIARHVLEGADQAALDEFRSNPIGTGPYVVESFSANDQVVYIPNENYRE
                     PNKPFFSRIILKGGGDAAAAARASIQTGEYDFAWNLAVEPELLRNMESDGNPGELVIY
                     GGLTIERINFNFSDPDTEVDGQRSEMNTPHPILSEHAVRQAFTIGVDREKIANEFYFG
                     APEEPAVANILSGIPSMESPNTELVYDPEKAAQLLDEAGWVMDGDVRKKDGKELELGL
                     YTTTSPVRQKIQAVVKSNLEAIGFRIGLEQVDSAVFFDSAVGNDQSNTHFYTDTNEFA
                     SGVGAPPPVSYMVRWYAGPNREEIAQKSNNWAGRNIQRYINEEYDALYEKARVEPDPE
                     KSAELFIEMNDILYFDYAVMPLVRYGSKAGVSKRLNQENLALSAYEYAYWNIANWNLA
                     EGVEG"
     gene            complement(3277..5157)
                     /locus_tag="VGR29_06350"
     CDS             complement(3277..5157)
                     /locus_tag="VGR29_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2073249.1"
                     /translation="MASQNHASGPLRALYEGLKAGNIDRRTFVHRASLLGVSAASAVF
                     LANTAGVAAQDASPAATAAAGSAERPAAGTENQERGAGGELKLIQWQAPTLLSPHVAT
                     GVKDFLASVLVLEPLMHYLPDATMIPNLVTEVPSVENGSLSEDLTEVTLKLLPDVVWS
                     DGEPFTAEDVVFTIDWVKNPENASVNINTFETIESAEAVDEHTVKVTFAESNPLWFDP
                     FTGTSTGFVYPRHVLEGGPEAHEAFLSSPVGTGPYVVESFTPNDQATYVINENYREPN
                     KPFFSRVQLKGGGDAPAAARSVLQTGEYHFAWNLQVEPNVLSDMEAEEAPGALVPYPG
                     VSIERINFNFSDPWTEVDGQRSHFGTPHPFLTDDAVREAIATAIDREQIANEFYGQGQ
                     QPATNILYGDPETESENTTREFDPEKAAQLLDEAGWTLNGDVRSKDGVELSAVYATSV
                     NSVRQKTQAVVKSNLEAIGIKVQLEQIDAGIYFDGSAGNEQNINHFYWDMCMYQGVPN
                     SPRPLSFFETWYAGPDGQNVAQAANEWNGQNNQRWQNEEYDAVFEQAQTETDPDAFAD
                     LFIQMNDLVINNHVIVPLVVVGSARGVSKALRVENIALAPFSYDYWNIANWNLAEGAE
                     LS"
     gene            complement(5590..>6533)
                     /locus_tag="VGR29_06355"
     CDS             complement(5590..>6533)
                     /locus_tag="VGR29_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2073250.1"
                     /translation="LQQIDEEGDQGTIEVARGASVERLHINFSDPNTEVDGQRSHLGT
                     PHPFLTDDAVRQAMNLAADRETIATEFYGEGQPATANILTGLELFESPNTSWEFNLEK
                     AAQILDEAGWVMEGDVRAKDGVELSVVYATSVNAVRQKTQAVIKQAFEQLGIRTQLEQ
                     VDAGIFFDSGAGNEQNISHFYRDIQMYTNSPSSPVPVEFMTSWYAGPNGENVAQKSNS
                     WQGQNYQRWVNEEFDQLYEDLQVATDIEAAAQLLIQLNDVLISNVVIIPLVNRSVDTY
                     AYSNLLRPENLELGPFHDVTYWNIANWNFAEGVEPQS"
BASE COUNT         1280 a         1976 c         1956 g         1321 t
ORIGIN      
        1 agggtctacc ggtcgacgaa tcattagaag tcgcagatcg ctctatcact ttggcggaca
       61 gccgctacgg ggatcatctc gcactccgct gggtcactga ttccataggc aaatgagaag
      121 tacctccgta gtctccccgg acacgtgcag acgagggcga cggttcgccg cgcaataaaa
      181 cccccgacta cgctttcacc agccgagggt gtggttccgg gctgggacac cttcggacca
      241 cgtagggagg tatagcactc caaacccgtc actcatgggc acgggtcgtg cgggccctca
      301 gtcaggaagc cgacccacgc tgtgactttc atggtgctga ctttgcttcc gaaagagagg
      361 tccatgtatg aagatattga cgagcaaagg catggcaggc cttcgtgcag ctgcgcttgc
      421 gctgctgctg ggagccttca tgacaggagg ggtcgtagcc caggacaccc ctgcagcgct
      481 ggcacaagtc gatgaggcag ccgaggaagt tgaggacgag ggcttcgacg actggggact
      541 tctgggtctt ctcgggcttg ccggtctggc agggctgatg aagcgaaacc cggagccggt
      601 cgtgcatacc gagcttgatc gagaccgacc gcgcgtctag gcgatagacg gctacccgat
      661 cgatgtcttt atgacgacga tcgtggctta cggacagaag gtcatccctc ttggggatga
      721 ccttctgtcg tgtcaaaaat ctccgggcaa acccaggacg acgcttcggg ggaaggggag
      781 cccggtacca ggcaggcaag agtcgctgcc ttcctgacag ctgcttcgaa ataccgcatg
      841 ggaattgccg gattcccttg agtacgggtt ggccactgcc aggtaacctc atgctttcca
      901 taagcggatg aaattgattc tcctgaaaaa caatgccggg acagagattc cctccccggc
      961 atcacgctgt gctgtggcaa cgagagaaag ttacccctcc acgccctcgg cgagattcca
     1021 gttcgcgatg ttccagtagg cgtattcata tgcgctcaag gcgaggttct cctgattgag
     1081 ccgctttgag acccctgcct tcgacccgta ccgcaccagc ggcatgaccg cgtagtcgaa
     1141 gtacaggatg tcgttcattt cgatgaagag ctcagccgac ttctcgggat ccggctcgac
     1201 ccgggccttc tcgtaaaggg catcgtactc ctcgttgata tagcgctgga tattgcggcc
     1261 cgcccagtta ttggacttct gcgcaatctc ctcgcggttc ggccccgcat accagcgaac
     1321 catgtacgac actggcggcg gcgcgccgac gccggacgcg aactcgttgg tatccgtgta
     1381 gaaatgggtg ttgctctggt cgttgcccac cgcactgtcg aagaagaccg cggagtcgac
     1441 ctgctccagc ccgatccgga atccgattgc ctcgaggttg gactttacaa cggcctggat
     1501 cttctgccgc acggggctcg tcgtcgtgta gaggccgagc tcaagctcct tgccatcctt
     1561 cttgcggaca tcgccatcca tcacccagcc cgcttcatca agcaactggg cagctttctc
     1621 cggatcgtac accagctcgg tgttgggcga ctccatcgac ggaatgccgg acaggatgtt
     1681 ggcgacggcc ggctcctcgg gggcaccgaa gtagaactca ttggcaatct tctcgcggtc
     1741 aacgccgatc gtgaacgcct ggcgaactgc atgctcgctg aggatcggat gtggggtgtt
     1801 catctcggaa cgctgaccat cgacctcggt atcagggtcc gagaagttga agttgatgcg
     1861 ctcgatcgtc agaccaccgt agatgaccag ctcgcccgga ttgccatccg actccatatt
     1921 gcgcagcagt tcaggctcaa ccgctaggtt ccaggcaaag tcgtactcgc cggtctgtat
     1981 ggaggcccga gcagccgctg ccgcatcacc accgcccttg aggatgatcc gggagaagaa
     2041 cggcttattc ggctcgcggt aattctcgtt ggggatgtag acaacctgat cattggccga
     2101 gaacgactcg accacataag gtccggtccc gatcgggttg gagcgaaact cgtccagcgc
     2161 ggcctgatct gccccttcaa gcacatgcct ggcgatcacg atggaggcgc ccatgcccgt
     2221 gaaggcgtcg gcccacgacg ggttggaatt cgcaaaggtc accttcgccg tcagctcgtc
     2281 aacgacctcg atatccttga tcaactcgta tacattctgg agcaccgcgc cattggcgtc
     2341 atccagcgcc cattcccaag tgaacctgac atcctcagcg gtgaacggct cgccgtcgct
     2401 ccacagcacg cctgtcttaa gattgaaggt gacgctcttg agatcctcgg caagcaggcc
     2461 gttatcgacg gtggggactt ccgtgacaag ttggggcgca agggcgccgt ccggcagacg
     2521 caccattagg gattcggaga cgaagagtgc cgccaggttg tccttgtcgc ccgttgccac
     2581 cataccgttc agctgggacg gcgcctgcca ctgcaggacc ttgagctcac cgcccgcacc
     2641 gcgctcctga ttttccgtgc ctgccgccgg gatggtgcct acaccggcga cccgctcgga
     2701 ctccggcgat gcagcgggag aggactcctg ggccgtcacg gtattggcgc agtacatggc
     2761 gacgctgagg cccatgccaa gggccgtggc acgctcgatg aactcgcgac gtgacaattc
     2821 tccctgcttg agcgatgtga acagcgccga atatgggccg ttccggaaat ctgaagacat
     2881 tgccggttct ccttgcgata gtgtgaccgt gggccgtccc acagcccgtt cgcgacccgc
     2941 gcgtttcccc tcaaggggat tatgtcagcg caccgctatt ctatccggtc cgcgccacat
     3001 actacagata ttgggttgga ctgaaaaaac tcacgacggc gttgcgccgg tccccgttgt
     3061 caggcgcttt cacattccgc agcgataccc ggaaccgccg ggacgtcacc cggttgctgt
     3121 ggcctgagta tgggacatga atgaagcctg cgcggatgtg ccgcacaggc ttcctgagcc
     3181 caggagcgct cagcagaggc aactccggta cgggcccgta cggagtgccg gatagacgaa
     3241 accggccggg cactccatgg agagatatga gaaaccttag gagagctcgg cgccctccgc
     3301 aagattccag ttggcgatat tccagtagtc gtaggagaac ggcgcgagcg cgatgttctc
     3361 cacgcgcaag gccttggaga caccacgcgc agagccaacg acgaccagcg gcacaatgac
     3421 atggttgttg atcaccaggt cgttcatctg gatgaagaga tcggcaaatg cgtcaggatc
     3481 cgtctccgtc tgcgcctgct cgaagacagc gtcgtactcc tcgttttgcc aacgctggtt
     3541 gttctgacca ttccattcgt tggccgcttg ggcgacattc tgtccatcgg gccctgcgta
     3601 ccaggtttca aagaacgaga gcggacgggg cgagttcggg actccctggt acatgcacat
     3661 gtcccagtag aagtggttga tgttctgctc gttgccagcg gagccgtcga agtagatgcc
     3721 ggcgtcgatc tgctcgagct gaaccttaat gccgatcgcc tcaaggttgg atttgacgac
     3781 cgcctgcgtc ttctggcgca ccgagttgac gctggtcgcg tagaccgcgc tcagctccac
     3841 tccatccttg gaccggacat cgccattgag ggtccagcca gcctcgtcga ggagctgggc
     3901 cgctttctcc gggtcgaact cacgggttgt attctccgac tccgtctccg ggtccccgta
     3961 aaggatattg gtcgccggct gctgaccctg accatagaat tcgttggcga tctgctcgcg
     4021 gtcaatcgcc gtggcgatcg cttcacgcac cgcatcgtcc gtgaggaagg gatgcggcgt
     4081 gccgaaatgg gaccgctgac catcaacctc ggtccaggga tccgagaagt tgaagttgat
     4141 gcgctcaatg ctgacgcccg gatagggaac caaggcaccg ggggcttctt cagcctccat
     4201 gtcggacaac acgttgggct caacctggag attccaggca aagtggtact caccggtctg
     4261 gaggaccgaa cgcgcggcgg ccggggcgtc gccgccaccc ttgagctgca cgcggctgaa
     4321 gaagggcttg ttcggctccc ggtagttctc gttgatcaca taggtcgcct gatcgttcgg
     4381 cgtgaacgac tccacaacat atggaccggt cccgaccggc gacgacagga acgcctcgtg
     4441 cgcctcaggg cccccctcca acacatgcct gggatagaca aagccggtgc tggtgcccgt
     4501 gaaggggtcg aaccagagcg ggttggactc cgcaaacgtc accttcaccg tgtgctcatc
     4561 gactgcctcg gccgattcga tcgtctcaaa ggtgttgatg ttcacggagg cgttttccgg
     4621 gttcttcacc caatcgatag tgaacaccac gtcctctgcg gtgaaaggtt caccatcgct
     4681 ccagacaaca tctggcaaca gcttgagggt gacttccgtc aggtcctcac tcagtgagcc
     4741 gttttccacg gagggcactt cggtgacaag attcgggatc atcgtcgcat caggcagata
     4801 atgcatgagt ggctcgagca caagcaccga cgcgaggaaa tcctttacgc ctgtcgccac
     4861 atgcgggctg agcaatgtcg gcgcctgcca ctggatgagc ttcagctcgc caccggcgcc
     4921 gcgctcctgg ttctcggtgc cagcagccgg ccgctcggcg gagccagctg cagcagttgc
     4981 cgccggggaa gcatcctgcg cggcaacgcc ggccgtattc gccaagaaga ccgccgaagc
     5041 ggcactgaca ccaagcagtg atgcgcggtg gacgaaggta cgtcgatcaa tgtttcccgc
     5101 cttgaggccc tcgtagagcg ctcgcagcgg accggatgca tgattctgag aggccattct
     5161 cggtcgttcc tcctgccggc gtcgtaccgg tattgtgatc atcggggaaa gtgttccccg
     5221 atggagtccg agtcattatc cgggtctgtt ggctatgcga cgaccgggag catctggaag
     5281 gctaccggtt acccagatgt tgactcacgc gggaagttta atcatcccgg atgccattga
     5341 caatggcttg tgaaacaggg cacgaatgat gccgcaccaa ggacccaagc cttaccttgt
     5401 attaggagca caagttggcg taagtacacg agggcaccgg agccagcgga ccccccggct
     5461 gctgaatcgg acagcaagag cacgacaaga gaacgagcgg cgtcacgaca tgtgacgccg
     5521 ctcgttcttc atctttcgat catatgccct tcatccgcga cgggccgtcc cggataatgt
     5581 agctgaatgc taggactggg gctcaacgcc ttcggcgaag ttccagttgg cgatgttcca
     5641 gtacgtgaca tcgtggaacg gcccgagctc caggttctcc gggcgaagga ggttggagta
     5701 cgcgtaggtg tccactgaac ggttcaccag cgggatgatc accacattgc tgataaggac
     5761 gtcgttgagc tggatgagca actgcgccgc cgcttcaatg tcggtcgcca cctgcagatc
     5821 ctcatacagc tggtcgaact cctcgttcac ccatcgctgg taattctgtc cctgccagga
     5881 gttcgacttt tgggcgacgt tctcaccgtt cggaccagcg taccaggagg tcatgaactc
     5941 caccggcacc ggcgacgacg ggctgttggt gtacatctgg atgtcgcggt agaagtgtga
     6001 gatgttctgc tcgttgcccg cgccgctgtc gaagaagatg ccggcatcga cctgctcgag
     6061 ttgagtcctg atgccgagct gctcgaaagc ctgcttgatg acggcctgag tcttttggcg
     6121 aaccgcgttc acggaggtcg catagaccac gctcagttcc acgccgtcct tggcccggac
     6181 gtcgccttcc atgacccatc cggcctcatc aaggatctgt gccgccttct ccaggttgaa
     6241 ctcccaagag gtattcgggg actcgaacag ctccagacca gtcaggatgt tcgcggtcgc
     6301 cggctgtccc tcaccgtaga actcggtcgc gatggtctcg cgatcggccg caaggttcat
     6361 cgcctgccgg accgcgtcat cggtcaggaa gggatgcggc gtaccgaggt gcgaccgctg
     6421 gccatcgacc tcggtattgg ggtccgagaa gttgatgtgg agacgctcga ccgaggcacc
     6481 ccgggccacc tcaatggtac cctggtcacc ctcctcatcg atctgctgca gga
//
LOCUS       DASYWM010000157         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_475282, whole genome shotgun sequence.
ACCESSION   DASYWM010000157 DASYWM010000000
VERSION     DASYWM010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475282"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            461..1120
                     /locus_tag="VGR29_06360"
     CDS             461..1120
                     /locus_tag="VGR29_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_1061 family FMN-dependent PPOX-type
                     flavoprotein"
                     /protein_id="HEV2073251.1"
                     /translation="MQLTSDTPPYSESSFDPHAVTTIDDLVAIIGVPKESTANKQTNF
                     LTPLLIEFLQKSPFYLLATANVNGTCDVSPKGDPAGALYIPDERTVVLPDRLGNRRVD
                     SLKNIVTNPHVGLLFMIPGVDETLRLNGHARLTKDPALLEKMPMRGKLPNLAIVVEID
                     EVFTHCARSILRSQLWQPDSWADPSTIPTMAAMSAEQKQRPAPDESLGKRTEEYRSTL
                     Y"
     gene            complement(1117..1983)
                     /locus_tag="VGR29_06365"
     CDS             complement(1117..1983)
                     /locus_tag="VGR29_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2073252.1"
                     /translation="MTAPTPFPVIDSHQHLWDPARREYPWLTDPRIARPFGPEDLKPL
                     LEESGIDATVLVQTVPDMDETRELLQTAAEHEFIAGVVGWVDLTDSHLAHILTELKSG
                     PNGDRLVGIRHQVHDEPDDAWLAREDVQLGLATVANASLVYDVLIRPQHLQIAINIAE
                     DFPHLRFVIDHMAKPDIAGGGIEAWSAAMHEFAGMENVACKLSGILTEAGEDWTVDSL
                     RPYVRTVVEIFGPHRLMFGSDWPVSLLSADYASVYRTLRETLGDLNLTEDEMAAIFGR
                     TAIHWYGLSIQA"
     gene            complement(2092..2439)
                     /locus_tag="VGR29_06370"
     CDS             complement(2092..2439)
                     /locus_tag="VGR29_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEV2073253.1"
                     /translation="MKCPIDQADLVMTERQGVEIDYCPTCRGVWLDRGELDKIIDRSE
                     ELNAGRASAPVRGEFESPTVGGMHGKVRPLDSRDVYEGDRDRDYDYDDDRRRPGRRKK
                     KRESFLGDLFDFD"
     gene            complement(2525..3607)
                     /locus_tag="VGR29_06375"
     CDS             complement(2525..3607)
                     /locus_tag="VGR29_06375"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEV2073254.1"
                     /translation="MSNQTSRIEHAPYGEMNEQPVERFMLSNDSGMSVAILSFGAIVQ
                     ELRVPDARGTSANVVLGFDNLAQYGQKHPHFGAVIGRFANRIGGAQFPLDGTVHELTP
                     NKGTITAHGGAKGFDRYVWDAEVVELDHGPGVKLTHVSPDGDEGFPGELTATVTYSLS
                     ADNGLRLDYTATTTKPTVHNLTNHSYFNLGGEASGTIEDHILTVHATHYTPTRPDQVP
                     TGEIAPLAGTALDFSEPRPIGDAVRDGTDEQIRIARGVDHNYLVMQEEGSSDLIPAAR
                     IENPETGRTMDVLTTMPGMQVYTGNSLDGSISGYSGRLYRQTDAICFETQFFPNSPNH
                     PEFPSTELRPGEQFQSTTIYRFGTME"
     gene            complement(3706..>3910)
                     /locus_tag="VGR29_06380"
     CDS             complement(3706..>3910)
                     /locus_tag="VGR29_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073255.1"
                     /translation="VVSRRVSIQSAAISPGGHDGVVRDDIGRANGAFRHGFASAAKGS
                     LCFSPCGLSQVRPVQLGRPIVTP"
BASE COUNT          730 a         1218 c         1203 g          759 t
ORIGIN      
        1 tcgatctcct cgtccgcatc cacacccgat gcgtcggcca gcatcgccac gatctcgtga
       61 tcgtcaatga ccagatcagc gcggatctca tcgctgtcgg tgacaaggac accaccggtg
      121 ataacggtac gttgcgacat cgagcagaaa cccctccggc cgccaagcgg cgcgggaatg
      181 cgtgatcaac gtggcgcgtc aatatctcca tgcacgccga gtgtccccag acgctcccga
      241 tactacgcat acagccggca ttcgtccaca ttggagagtg atcggatgtc gcaaccatgt
      301 gttcgccagg tgctatgatc aagcaacctg tgagacgttc gacgggccac gtgacagact
      361 accggcactg gtggccgttg tcgtcgcggt tgccgccgtc tggaagaacg agcgtgcgta
      421 gccgggacga tagccgccca ggggagatga gggcatcacc atgcaattga cgagcgatac
      481 accgccatat tcggaatcat cgtttgaccc tcacgcggtg accacgatcg atgacctcgt
      541 ggcgatcatc ggtgtgccga aggaatcgac ggcgaacaag cagacgaact tcctgactcc
      601 gctcctgatc gagtttctcc agaagtctcc cttttacctg cttgccacgg cgaacgtgaa
      661 cggtacctgc gatgtgtccc cgaaagggga cccagccggt gccctgtaca tccccgatga
      721 gcgaacggtc gtgctccccg atcggctggg aaaccgccgc gtggattcgc tgaagaacat
      781 cgtgaccaat ccgcatgtcg gattgctgtt catgattccc ggggtcgatg aaacacttcg
      841 tctcaacggg catgcgaggc tcacgaagga tcctgccctc cttgagaaga tgccgatgcg
      901 aggcaaactg ccaaacctgg ccatcgtcgt cgagatcgac gaggtcttca cccattgcgc
      961 caggtcgatc ctccggtcac agctgtggca accggattcc tgggccgatc cgtcgacgat
     1021 cccgaccatg gcggcgatga gcgccgagca gaagcaacgc ccggcgccag acgagtctct
     1081 cggcaagcga accgaggaat accgctcgac cctgtattag gcctggatgg acaggccata
     1141 ccagtggatt gccgtcctgc cgaagatcgc cgccatctcg tcttcggtga gattgaggtc
     1201 acccagtgtc tcccggagcg tccggtagac actggcgtaa tccgccgaga ggaggctgac
     1261 gggccaatcg gagccgaaca tcaggcgatg cggcccgaag atctccacta cggttcgcac
     1321 gtagggccgc aggctgtcga cggtccagtc ttccccggcc tctgtcagga tccctgagag
     1381 cttgcaggcc acgttctcca tgccggcgaa ctcgtgcatg gcggcactcc atgcctcaat
     1441 gccgccgccg gcgatatcgg gtttcgccat gtggtcgatg acgaaccgga gatgcgggaa
     1501 gtcttcggcg atattgatcg caatctgcag gtgctgcggt cggatgagga cgtcgtagac
     1561 gaggctcgcg ttcgcgacgg tggcgagccc gagctggaca tcctcgcgcg cgagccaggc
     1621 atcgtcgggc tcgtcatgca cctgatggcg gatgccaacc agccgatcac cattcgggcc
     1681 ggatttcagc tctgtcaata tatgcgcgag gtgcgagtca gtcaggtcga cccaaccgac
     1741 aacgccggcg atgaactcgt gctcggcggc agtctgcagg agctcccgtg tctcatccat
     1801 gtcagggacc gtctgcacca gcacggtggc gtcgatacct gactcctcca gcaacggctt
     1861 caggtcctcg ggcccgaagg gcctcgcgat ccgggggtcg gtgagccagg gatactcccg
     1921 acgcgccgga tcccagagat gctggtggct gtcgataacg gggaacggcg tgggcgcggt
     1981 catgattctc tccgtttgct ggcgatcgag tggcatgcgg tcacgggcac acccatccgc
     2041 cacgcgatac gctggcacgc ctgacggctt ctgcttcgtt ccggggccct gctagtcaaa
     2101 gtcgaagaga tcccccagga aggattcacg cttcttcttg cggcgcccgg gccgacgcct
     2161 gtcgtcgtca tagtcataat ccctgtcccg atccccctcg tagacatcgc gggagtccag
     2221 cggacggacc ttaccatgca tgccgccgac ggtcggggac tcaaattcgc cacgaaccgg
     2281 ggcagatgcc cgccccgcgt tcagctcctc cgagcggtcg atgatcttgt cgagctcacc
     2341 gcggtccagc cagacaccgc ggcatgtcgg gcaataatcg atctccacac cctgacgctc
     2401 ggtcatcacg aggtctgcct ggtcaatggg gcatttcatc cttgatatct ccttgcatac
     2461 gtcattgcaa cagatgcgag cgccacatgg gccactcgct atatgattcg attcctcgca
     2521 acaatcactc catcgtaccg aaacgataga tcgtggtgga ttggaattgc tcgcccggtc
     2581 gtagctccgt ggaagggaac tcggggtgat tcggtgagtt cgggaagaac tgcgtctcga
     2641 agcagatcgc gtcggtctgg cggtagagac ggccgctgta accgctgatc gatccatcca
     2701 gcgagttgcc ggtgtagacc tgcatccctg gcatggtcgt cagcacatcc atagtccgcc
     2761 cggtttccgg attctcgatc cgcgcggccg ggatgagatc ggatgagccc tcctcctgca
     2821 tcacgaggta gttgtgatca acgccacgcg caatccggat ctgctcgtcg gtgccgtccc
     2881 gaacggcatc cccgatggga cgcggttcgg aaaagtcgag ggcagtgccg gcgagcggtg
     2941 cgatctcccc ggtcggaacc tggtccggcc tcgtgggcgt gtaatgggtc gcatggacag
     3001 tgaggatgtg atcctcgatc gtgccggacg cctcgccccc gaggttgaag taggagtggt
     3061 tggtcaggtt gtggaccgta ggtttcgttg tagtggccgt gtagtccagg cgcaggccgt
     3121 tgtccgccga cagagagtag gtgaccgtgg cggtgagctc ccctgggaag ccctcgtcgc
     3181 catctgggct gacgtgcgtc aactttactc caggaccatg atccagctcg acgacttcgg
     3241 catcccagac gtagcgatcg aagccctttg cgccgccgtg ggcggtgatg gttcccttgt
     3301 ttggcgtcag ctcatggacg gtgccgtcca gcgggaactg tgccccgccg atccggttgg
     3361 cgaagcggcc gatcaccgcg ccgaagtggg gatgcttctg cccgtactgg gcgaggttgt
     3421 cgaaacccag caccacattg gcgcttgtgc cccgggcatc aggcacgcga agctcctgga
     3481 cgatcgcccc gaatgagagg atcgccacgg acatgccgct gtcgttggag agcatgaagc
     3541 gctcgaccgg ctgctcattc atctcgccat agggtgcatg ctcgatccgg gatgtctggt
     3601 tgctcatgat atcgcctttc acgatgtcct tttcgtgggt ctattgtacg gggaggggac
     3661 attggcggac ggtatgcccc tacgctcgcc gcagtcaatc ggcattcagg gcgtgacaat
     3721 gggcctcccc aactgtacgg gacgtacttg gctgagcccg cagggcgaaa agcagagcga
     3781 gcccttggcc gcgctcgcga acccatgccg gaatgcccca ttggcgcggc caatgtcatc
     3841 cctcaccacg ccatcatgcc cgcctgggct gattgccgcg gattggatcg acacccttcg
     3901 ggatacgacc
//
LOCUS       DASYWM010000158         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_456896, whole genome shotgun sequence.
ACCESSION   DASYWM010000158 DASYWM010000000
VERSION     DASYWM010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456896"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VGR29_06385"
     CDS             complement(<1..1212)
                     /locus_tag="VGR29_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2073256.1"
                     /translation="MGIGNVLGKGNDASASDRPPEDGRDRRAGPGDGAGDGVPREEIP
                     DHIFPALDDQDDADDDLSYIHPIVGKTDHTPESEKTVTRIEVPVRTIVTVALALFLIW
                     LLIQVWGILLQVFLAFLLATALLPLVKRLENRGMPKGIASSLVFLGLIAMIAGFFGII
                     VPPLVEQGQNIVTNAPEYLNRFEGFIERYPSLEEQYERLRDQGLNGNGEGTEQGAAGA
                     AAVPVNQVVEVSADIVTRLANTFFVLILTFYLMLEGERTWRFLSRYFTPGLRYRMRKA
                     YPEITHVVSGYVVGQAINSASFGIFAFITLTILGVPETLLLAVLAAVFDAVPIVGVPV
                     ATIPAVLIALTVSWQTAAIVLAAYLIYQQFENYVMVPRVFGNTLQVSSLSILVGVLIG
                     GQLLGVIGIILS"
     gene            complement(1436..2812)
                     /locus_tag="VGR29_06390"
     CDS             complement(1436..2812)
                     /locus_tag="VGR29_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2073257.1"
                     /translation="MTTFHSDAAGHRAHDLTSRSPQRNGRGLYRVVTFLTLFALLFGG
                     FVLMAPISASAQSTATTTAYLNLRTGPNTSTAIITVMPYGATVNINGGAESGFYPVSY
                     GQYSGYASADYLSIGGSSSGNATNGATGTAYVVDGSLNLRSGPGLSYAVLTVMPNGAA
                     VSLTGESGNGFLGVVYNGTSGFASATYLSTSGNAPASAPQAPSGNVTGSATVNVSALN
                     LRSGAGTSYGVVAVMPQGASVSLTGDSSNGFAGVIYNGVSGWAFAEYLSTGGSTPAPA
                     PAPAPAAGSGSVAVGDTVVNSGYVSTNALNLRSGPATSYGVITVMYSGASVEIMGDPQ
                     GGFYPLRYNGTKGWASGDYLTIGSSAPVAPAPGGSTSDIVAIIYAAADRYGQSREDML
                     RVARCESVLDPSAVNPYSNASGLFQFLPSTWATTPYANQDIFDPVANANAAGWMWSVG
                     RRNEWVCQ"
BASE COUNT          659 a          933 c          953 g          551 t
ORIGIN      
        1 cgacaggatg atcccgatca cccccaacag ctgtccaccg atcaacacac ccacgagaat
       61 gctgagcgac gacacctgca aggtgttacc gaagacgcgt ggcaccatga cgtagttctc
      121 gaactgctgg taaatcaagt aggcggccag cacgatcgcc gctgtctgcc atgacaccgt
      181 cagcgcaatg agcacggcgg ggatggtcgc gacaggcact ccgacaatcg gcaccgcatc
      241 gaagaccgcc gccagcaccg cgagcagcag cgtctcgggc acaccgagaa tggtcagcgt
      301 gataaatgca aagatgccaa atgaggccga gttgattgcc tggccgacaa catagccgct
      361 gaccacatgg gtgatctcgg gatacgcctt tcgcatgcga tagcgaaggc cgggagtgaa
      421 gtagcgagag aggaacctcc aggtccgctc cccttcaagc atcagataga acgtgagaat
      481 gaggacaaag aaggtattgg cgaggcgagt gacaatgtct gcgctgacct cgaccacctg
      541 gttaacgggt acggcggcgg cgccggcagc gccctgctca gtgccttccc catttccgtt
      601 caagccctga tccctgaggc gctcatactg ctcctccagc gagggatagc gctcgataaa
      661 cccttcgaac cggttgaggt actccggagc attcgtgact atgttctgcc cctgctcgac
      721 gaggggcggc acgatgatgc caaagaaccc ggcgatcatg gcgataagac cgagaaagac
      781 cagcgacgag gcgatgccct ttggcatgcc cctgttctcc agccgcttga ccagcggtag
      841 cagggctgta gcgagcagga acgccagaaa gacctgaagc agaatacccc agacctggat
      901 cagcaaccag atgaggaaga gagcgagcgc aacggtgacg atcgtccgca cgggaacttc
      961 gatacgagtg acggtttttt ccgactccgg cgtgtgatct gtcttgccga caatcggatg
     1021 gatgtacgag aggtcatcgt cagcatcatc ctggtcatcc aacgcgggga agatgtgatc
     1081 ggggatctcc tcccgaggca ccccgtcgcc ggctccgtcg cccggtccgg cgcggcgatc
     1141 tcgcccgtct tccggcggcc ggtccgacgc tgacgcatcg ttgcccttgc cgagtacatt
     1201 cccgattccc attgaaccac tccaagtgtc tgacttctga atgatgcccg cgactcgtct
     1261 ctacatgggc acgacaaacc tcctgcctat tgcaggaggt ttgtcgattg ctatgcaact
     1321 attgtgcccg gagcggagat cagaagttgt gcccgctggt taaacccgga ttaccgctcc
     1381 cggcgatccg tttcgggaac gagggtgtat cactctcggt gatacaccct ccggcctatt
     1441 ggcaaaccca ctcattacga cgtccgaccg accacatcca gcctgcggcg ttggcattcg
     1501 ccaccgggtc aaagatgtcc tgattggcgt acggagttgt cgcccacgtg cttggcagga
     1561 actggaaaag gccgctcgcg ttactgtacg ggttgaccgc gctcggatcg agcaccgact
     1621 cgcaccgtgc aacgcggagc atgtcctccc gggactgacc gtagcgatcg gcggcagcat
     1681 agatgattgc cacaatatcg gaggtgctgc cacccggcgc aggcgcgacc ggcgccgagg
     1741 agccaatggt caggtaatca ccgctggccc accccttggt gccgttgtaa cgaagcgggt
     1801 agaagccgcc ctggggatca cccatgatct cgaccgaggc gccgctgtac atcacggtga
     1861 tgacgccgta actggtcgct ggaccgctgc gcaggttcaa cgcattggtg ctgacgtatc
     1921 ccgagttcac gaccgtatcg ccgaccgcga cactgcctga gcctgctgca ggagccggag
     1981 ccggggcagg cgccggagtt gagccacccg tgctgaggta ctcagcaaag gcccacccgg
     2041 agacgccgtt atagatgacg cccgcgaatc cattgctgct gtcgccggtg agggagacgc
     2101 ttgcaccctg cggcataact gccacaacgc catagctggt gcccgcgccg ctgcggaggt
     2161 tgagcgcgga gacgttcacc gtggcagagc cagtcacatt gccgctcggc gcctggggtg
     2221 ccgaggccgg ggcgttgccg gaggtgctca ggtaggtggc ggaagcaaag cccgaggtgc
     2281 cgttgtagac gacaccgagg aagccattgc cggactcgcc cgtgagcgac accgcggcgc
     2341 cgttcggcat cacggtgagc actgcatagc tcaatccagg gccgctgcgc aggttcaagc
     2401 tgccatcaac gacataggct gtgcccgtcg cgccgttcgt ggcgttcccg ctggagctac
     2461 ccccgatgga gaggtagtca gcactggcat agccggaata ctgaccgtac gagacagggt
     2521 agaaaccgct ctctgcaccg ccattgatgt tcaccgttgc accatagggc atcacggtga
     2581 tgatcgccgt gctggtgttt ggacccgttc gcaggttgag atacgcggtc gtggtcgcag
     2641 tcgactgtgc cgaggccgag atcggggcca tcaggacaaa accgccaaag agcagtgcga
     2701 acagcgtcag gaaggtaacg acgcgataga gcccgcgacc attacgttgt ggagaacggg
     2761 aagtgagatc gtgtgcgcga tgcccagccg catcactatg gaaggtggtc accaatggtg
     2821 ctcctttgga ccaggtactc cgcaaggcag atacgcaatg tgcgcatgcc atggtgcaac
     2881 gaccatggtg cccctggaat cctggccagt gtgtagaacg aaagaccgat gggtagcgcg
     2941 atcaccgtcc ctccaacggc atcgggcctg aaatgcttcc cctttgtcct tgatgggaaa
     3001 gggtgttgtg aggacgccgg cttttgggta ctacacgggt attccagcca agaacacgcg
     3061 catgtaaagc ctgagcaggg gaaccataat ctacgg
//
LOCUS       DASYWM010000159        17689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_193934, whole genome shotgun sequence.
ACCESSION   DASYWM010000159 DASYWM010000000
VERSION     DASYWM010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 17689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17689
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193934"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VGR29_06395"
     CDS             <1..266
                     /locus_tag="VGR29_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073258.1"
                     /translation="ERLRSRIAELEHAELERQRAHAALLMRVQQQAAVAKLGIRALAV
                     SDTNVLFSEAVCVLRKTLDVDYAKVLELLQGGEEHRGGFGVVK"
     gene            complement(585..1091)
                     /locus_tag="VGR29_06400"
     CDS             complement(585..1091)
                     /locus_tag="VGR29_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073259.1"
                     /translation="MVRFTTRCCALLVLSLALLTAMVPASSLAQVPPATAACVGHGYK
                     EVRRTDGTSFKNPGECLKYVKDDGTLIAGPRMVIDVYYRDEDHCEFTVDLYGMTPGTR
                     VPVTIVHPVAGLQTIYTQIGTDGSTWYPTGGGTGAGFSIPIEHTVTVTAEYTELGSTA
                     VLTQDVRC"
     gene            2015..2263
                     /locus_tag="VGR29_06405"
     CDS             2015..2263
                     /locus_tag="VGR29_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073260.1"
                     /translation="MTTKRRRGHNEGSIYQRNDGRFVGAISLGMVDGKRRRKVIYGDT
                     REEVRQQLVKIHNDSAAAFLSRPPAHRRQTSSRNGSRA"
     gene            2467..3168
                     /locus_tag="VGR29_06410"
     CDS             2467..3168
                     /locus_tag="VGR29_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV2073261.1"
                     /translation="MRQDLVQRNIATLVHPPRILEYEAHNLTPDEANRLLAVVRGDRL
                     EALYLVSLILGLRQGEALGLRWEDVNLAAGTMTIRRQLQVINGQPTFTEPKTQRSRRT
                     IPLPAPVIDMLTRHRTRQKEDRLAAGGRWQSQWGLVFADELGAPASLSALRRNFRDAR
                     TRADLPHMRWHDLRHATSSFLAARGVHPIVAQAILGHASLATTLSVYTHARADANREA
                     TDLLGDLARVGTGTN"
     gene            complement(3749..5284)
                     /locus_tag="VGR29_06415"
     CDS             complement(3749..5284)
                     /locus_tag="VGR29_06415"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEV2073262.1"
                     /translation="MPEIDLEDAVTPLVMAVDIGSSSVRALLYDTAGRVVTGTEHQIR
                     HALETSPDGGSTADPDHLVSLVHSCIDHALANTGIRRGDIRAVGLTSFWHSLMGLDED
                     REPATHVLMWADKRSGDDALALERALDADRIHTETGCRLHSSYWPAKLRWLRRTDPDA
                     YASTKTWVSFVDYLSWTIHHELSTSISMASGTGLLHSSGAHWHDELLTVLEITIEQLP
                     KLTDRDVAFPPPIEEFRVRWPDLANAAWHPAIGDGAAANVGAGCVGADRIALTIGTSG
                     AMRVIIGDETDHEPVSQPLSPKLWQYRLDRRHRVAGGALSNGGNITYWLADLTQDHDF
                     DALTEAASAIEADGHGLTVLPFFAGERSPSWNDQLTGTISGLSLGTTAGELFRAFLEA
                     TAYRFASIYEDLRALALADHDIHANGGAALRSPLWLQILSDSLNHQIHALDAEAEASA
                     RGAAISALESIRAIDSLLEPETTIVRSYEPDENRHAIYVAGRQRLERYERALVKLGST
                     GVV"
     gene            5424..6233
                     /gene="cofE"
                     /locus_tag="VGR29_06420"
     CDS             5424..6233
                     /gene="cofE"
                     /locus_tag="VGR29_06420"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEV2073263.1"
                     /translation="MGDVESTGRAEKESVSILPIDGIGNVGPGDPLEQMIGDAIAESV
                     GELRWGDVVVVTHKIVSKAEGQLVDLGTVEPSPFALDYAATWGKDPRQVEMVLRQSKR
                     IIRMHRGIIIAETEHGFVCANAGVDASNAGQDMVCLLPRDPDASAARILGALQGRFFP
                     GAAAEDQPIGVIITDSFGRPWRNGIVNVAIGIAGISPLADYRGQFDPDGYELRASILA
                     VGDELAAAAELVMHKIAMRPVAVIRGYERQPGPPSGTGQDLVMPEERNLFP"
     gene            6275..7258
                     /locus_tag="VGR29_06425"
     CDS             6275..7258
                     /locus_tag="VGR29_06425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV2073264.1"
                     /translation="MSNLGYAASFEQFHPNDLLEYCKQAEKVGFGVVTASDHFHPWVP
                     SQGQSAFVWSWMGALGQATSLRFGTGVTPPGWRYHPAIIAQAAATLEAMYPGRFFLGL
                     GAGEALNEHIVGDYWPEAPTRLERLAEAIEIIEKLFTGKVIKHQGKHFRLESARLYTL
                     PENPPPIYVATSGPIMAGRTGKLTDGIITVGAADEKLKMLMERFEKGAREAGKDPSSM
                     PRILQVKVSYAKTDEEAIDSAVRDWPNGGMNFPKADIRNPEDFEAMAKLVRPENFKNR
                     VLATADLDKHVEYIQHFIDLGFGEIHVHNVNRDQEQFIEAYGKNVLPKLSW"
     gene            complement(7506..8273)
                     /locus_tag="VGR29_06430"
     CDS             complement(7506..8273)
                     /locus_tag="VGR29_06430"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEV2073265.1"
                     /translation="MTDHVPTPSAKSAASLQDPDGTRQAPPPNTGTTQGWMRGALALV
                     VAFAIFGSAWLVGERQGWSEIGRGGINATLLPEVGKPAPELFTMSSTGEPVLLSQLRG
                     EPVWINFWGSWCPPCRAEMPDVQKAYNALAPQGLHVLSIAMQESPEDAVRYRDSAGAT
                     FPVYVDPTRIASMIDSTEQPALAQQLALMTRDWQIANFPTHVFIDENGIIQSIVLSQM
                     TYEEAVEHGEAVLKTAHRLMPPAVIRQSARNDIRSNR"
     gene            complement(8280..9182)
                     /gene="ccsB"
                     /locus_tag="VGR29_06435"
     CDS             complement(8280..9182)
                     /gene="ccsB"
                     /locus_tag="VGR29_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEV2073266.1"
                     /translation="MDSLVRLSFYCFAASSFSLIAAAVMYIVFAVGRVRVLKQTMATS
                     TGHSVDSNSVHHEAGPIGFARWGTMLTVFGALFSTLAVVLRWGAAGHFPLTNMYEFSL
                     VFVSFLSILHLVFERMYKVRQLGAITTSVAAAMTLYIWSLDPAMREVNPLIPALQSNL
                     ILTFHVGAAVLSYATFTVAFGAATLFLIATRWSTPWLPSPEMLDDIAFRAITIGFPLF
                     TINLILGSVWAHEAWGVYWQWDPKETSALFTWLVYGVYLHTRSLRGWRGTRSAYVLLF
                     GFAATIFTYYGNYFFGGLHAYGGV"
     gene            complement(9259..10740)
                     /locus_tag="VGR29_06440"
     CDS             complement(9259..10740)
                     /locus_tag="VGR29_06440"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEV2073267.1"
                     /translation="MADVTARSQPKHSRPMLEVLVDRIWRFFCSVRAAIAEIAFLTLL
                     VLIGTLRGSSVPRAIADLIPPLEGFVDRWYAWDVFRSSLFAGILTLLTVAIIVCTINR
                     IPGIWATITHPKITTTPGFLAGAETSATFTSASSVDDLMATTSALLRTQRYRVLTEKV
                     GDRTHFYADKNRLSKLGTFPFHLGLILLLVGGIVTSVYGFRETEFIIPEGSRRNVGHG
                     TGLSVELNRFQDSWTEVGIPMEYRSEITVYEDGQAVKSGDVTVNHPMSYRNATFYQSS
                     FGNAAQMTITDGDGNIVYRDAAELGIFTMRGNPDAPAGLIRLPSEGLQLTLVAPDSNP
                     TNRPELDAMALESGQLWMGVQPIDGSATGNDIGQGGVVLTQGKPAKIGDLSVTFDRET
                     RFTVLQVGYNPGIPIFIIASVALLSGLAFTFYFPHRRVRGIISPGVDGAVLTMAPLAK
                     RDWSGKRDFFKFLEKADDVIGVKPDVKHPANAGDYEYLRRQNA"
     gene            complement(10782..11531)
                     /locus_tag="VGR29_06445"
     CDS             complement(10782..11531)
                     /locus_tag="VGR29_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEV2073268.1"
                     /translation="MTTNVSLLAAFAAGILSISSPCVLPLLPIYLTHIAGLSVGQSQQ
                     TARPVVMRNALAYVVGFSLVFIAFGAAFGAAGTFAGSLDFFSTNREWLVRIGGVLLVA
                     LGLHQIGIFRIPFLYRSAHMSLDGGRPGTVGSSFIIGVGFGAGWSPCMGPILGAILTM
                     AAGQGDIKRAVVLLTLYAAGLAVPFLLAAMAIGSLPQVLRRINKHLHLVTAASGAVML
                     GVGMIMLLGIYESLFTEIIRVAPWTPWEPEL"
     gene            11728..12522
                     /gene="recO"
                     /locus_tag="VGR29_06450"
     CDS             11728..12522
                     /gene="recO"
                     /locus_tag="VGR29_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV2073269.1"
                     /translation="MTDSMREKLYRSEAIVLSRMDYGEADRILVILTPARGKLDVIAK
                     GARKAQSRLGPQLDYFARVNLELARGRELDVVRSVESIDRFALLRENLEAFGHASYFV
                     ELVRNLTQPREEHRELYDLLARSLVLLNEKVDPWAIARHFELALLTALGYRPELFHCV
                     NCRQPIQPEVNAFSAELGGLLCPRCRNEDLASIPLSLNAQKYLRLLERGGLAAAARIK
                     PGPADKIEIQQVTSHYVRHVAERDFHSLRVLGALQQATLELAPGSG"
     gene            complement(12519..13235)
                     /gene="trmD"
                     /locus_tag="VGR29_06455"
     CDS             complement(12519..13235)
                     /gene="trmD"
                     /locus_tag="VGR29_06455"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV2073270.1"
                     /translation="MIIRPALRIDIFTLFPPMFEGVFGESIIKRAQAKGIIEIKAHDI
                     RDWTYDRHHTADDTPYGGGAGMVMKAPPIIEAVEEILGDDLDHAHIAIMSAGGRRFTQ
                     AIAQELSTAGRIALICGHYEGIDERVSQILHADEISVGDYVLTGGELPAMVIADAIIR
                     LVPGVITEASVLDESHAGSGVTGVEYPHYTRPAEYRNIPVPDVLLSGNHARIDQWRQE
                     QAKIRTHRWRPDLLGDTSSD"
     gene            complement(13232..13969)
                     /locus_tag="VGR29_06460"
     CDS             complement(13232..13969)
                     /locus_tag="VGR29_06460"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2073271.1"
                     /translation="MKNLVTFDFHNTLATCDPWFYLEIRDLPVDVLSHIDPALLHANT
                     PEEITARYRSLRQGVIASGREIDAADCVARIAAELDIEVDRASIEEGVHRLMRQAMEH
                     VEPVPGAIEVVREIAGRGIPVGVISSAVYHPFLEWTLEHFDIARDLAFVVTSASCGHY
                     KSNPEIYRHAMSLVGSDPSCSIHVGDSPKWDVWAAQQAGMRAIWFSNGDMDAFVDRAP
                     DTTPDYTANALSETAPWIYRYLDMPLT"
     gene            14268..14987
                     /locus_tag="VGR29_06465"
     CDS             14268..14987
                     /locus_tag="VGR29_06465"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEV2073272.1"
                     /translation="MPTALTIDVGFAKTEKYASRESGDTVELVERPGGGFSIVMIDGQ
                     GSGKAAKTLSLLLSSKAVGLIKEGVRDGVVARATHDHLFAFRHGRVSATFDLLSVDMQ
                     TKSIVVSRNAESPMLICDDGGHRVVPVDSGPMGRYQWTKPTVVELPMKAGMSVALFTD
                     GIPHSGRRAMLPPIDFDALAEATMRGGGPASDVAEAILQKAIERDQGRPGDDMAVIAL
                     SIVEQEPDMLIRRMSAQFPMA"
     gene            15032..15403
                     /locus_tag="VGR29_06470"
     CDS             15032..15403
                     /locus_tag="VGR29_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073273.1"
                     /translation="MNQETHPVDPRRIVVVGPCGAGKTTLVDHLREQGYDARVVAQEH
                     SIIRDLWRRREPDVVVALDLDLATLRGRRSPTWSADIFEKQHDRLREAFAAADIVIDT
                     GRHGEAEVVEMVKGWLNHHPR"
     gene            complement(15479..16138)
                     /locus_tag="VGR29_06475"
     CDS             complement(15479..16138)
                     /locus_tag="VGR29_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEV2073274.1"
                     /translation="MPSLLPLFPLGTVLLPGMPMPLHIFEPRYLQLMHDHGTDTIPFG
                     VALIHTERPPHRPWPAHSVGTAAMITGRTELPDGRWDLVVEGQQRFRIVDFDESQPYG
                     IATIEWLEEILGDPTEADALLRTVGAQFHRYAGGITRLTRRRFSGVMIPEEPVRASYD
                     LVSRLPLHTWERQRLLEAETAVERLRELSLLVERELALLLHAGAAGLAVNHPGSRFSL
                     N"
     gene            16542..17453
                     /locus_tag="VGR29_06480"
     CDS             16542..17453
                     /locus_tag="VGR29_06480"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2073275.1"
                     /translation="MMLKGIARGRSDPGADNGVRTLTPADIDLLAIRNHPRLEEGDAE
                     ALIIQAPGLSKWHPASREFLIVTPWRHRAEIPAVNVMSTFHHEDALMAAAIASAEKHG
                     AAAFVLLETYETRQPSFYARNGMQRLETIVTYELTQPRTFLRQITQRRQQFVKLDRQT
                     AELGQAVLNLDHVAFPWLWWNSAEEFSNYLSMPNIEVWAGILDGELISYVGFTHFWRW
                     SHLDRIAIRPDAQRQGFGREALHFAVERMLERGATRVGLSTQGENTASRNLYESIGFR
                     KTPGHDYDVHGVVFEPGRKYLEQGGQG"
     gene            17463..>17689
                     /locus_tag="VGR29_06485"
     CDS             17463..>17689
                     /locus_tag="VGR29_06485"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV2073276.1"
                     /translation="MGSSFGKIFKLTTWGESHGPALGVVVEGCPAGIPLTVDEIQHEL
                     NRRRVGQSKVTTPRNEKDQAELLSGVYEGITT"
BASE COUNT         3734 a         5029 c         5515 g         3411 t
ORIGIN      
        1 tggaacggtt gcggtcccga atcgcggaac tggagcatgc ggaactggag cggcagcgcg
       61 cgcatgcggc gttgctgatg cgagttcaac aacaggctgc ggtcgccaaa ctcgggatcc
      121 gcgcattggc ggtttcggac acgaacgttc tcttcagtga agctgtctgt gtcctcagga
      181 aaacgcttga cgtcgactac gcgaaggtgc tcgagctctt gcagggcggc gaggagcatc
      241 gcggcgggtt cggcgtggtt aaatgagaac gggaggatgg ttacatcctc gggtgtccct
      301 gctcaaccca atgagccagg ttctccgact gtgaccacca ctctgctgtc tcataccgcc
      361 ttccttcggc tgtctgggac cctcctgcat cttagcaaat cagcttggca aaaggaacct
      421 gtgccttggc tgaggaccaa aacggccccc gagtgtttct agcgaggccg cgccgttggc
      481 ttgcattgcg tgtgggcgac cttgcggcgt tctccgtggt tcgctgctcg tgctcgtatc
      541 cggtgccggc gatggagatc ggctccggcc cggagatggg gtcgtcagca ccgcacatcc
      601 tgggtcagca ccgcggtact ccccagctcg gtgtactctg ctgtgaccgt gacggtgtgc
      661 tcgatcggga tcgagaaccc ggccccggta cctccgccgg tcggatacca cgtcgatcca
      721 tccgtaccaa tctgcgtgta tatggtctgg agccccgcca cgggatgcac gattgtcacg
      781 ggcacacgcg tccccggtgt catcccgtac aggtccaccg tgaactcgca gtgatcctcg
      841 tcacggtagt acacgtcgat caccatgcgg ggcccggcga tgagcgttcc atcgtccttg
      901 acgtacttga ggcactcgcc gggattcttg aatgatgtgc cgtcggtgcg tcggacctcc
      961 ttgtacccgt gaccgacaca cgctgccgta gcgggcggca cctgtgccag actgctcgcc
     1021 ggcaccatgg cagtgaggag ggccaggctc aggacaagca gggcacagca acgcgtcgtg
     1081 aaacggacca tgacgatctc cttgtggccg tttgagcacg gtcacgcctt cgcacaaccg
     1141 atcgattgag tggaatcctg ctcggagcat agcaaccgat gaacgtaaaa gatagagccg
     1201 agccgggcac gacaacggcc ccccaaggta tcgagagccg tgtcgattgc ttccgtggca
     1261 tggtgaggct gctagcagca cctctcacga tctagtaact cgtgcacacg aaccctcctg
     1321 ctccatcctc cccgcagaaa tggttttcgg gacaacacac gtccttgcag catgtgatgc
     1381 tctggcatgc cccgtcacct cgacatgggg tgccggcagg accgcgatca caggtaaaca
     1441 cttgcacacc gttttcgtcc acatacccgg cgtcgctgca tacaccgctg cgacacccag
     1501 caccacttgt gcagcgcagg tccacctcgt tgcagcatcc attggcattt tggcattgtg
     1561 tggagaccac gcacatcgga tgaatcctgg actcagagcg gataaagccc aactgctcaa
     1621 taaccccagc gcaacatcca tgtcccaagg cgtgacggtc actacagtgg ggcttcctac
     1681 gaaggaaatc acctatgtga tcggatctga cggagagcca tacgccattg tagagactac
     1741 ccgggaggaa attcggctcc tttctacaga cgagtggagg aaatggtacc tgtctggtga
     1801 gagcacagcc gagcatgact tgtattgccg cggatcgtca ccgagacaga ctcgcgtaac
     1861 aactggccag ctcgacgtaa ccattattcc cagatcccat tcgcttaggt aaatgcgttg
     1921 ggaaggaacg attccggcaa cgtcggggat agtccgaata atcagaaagt ccgaacgagg
     1981 tctgcacttt atctgtgcgc acagggagac ggaaatgacg accaagcgcc ggcgaggcca
     2041 caatgaagga tcgatctacc agcgcaatga tgggcgattc gtcggtgcca tctcgttggg
     2101 tatggtcgac ggtaagcgcc ggcggaaggt catctacgga gatacccgcg aagaggtgcg
     2161 ccagcaactg gtgaagatcc acaacgactc cgccgcggcc ttcctgtcca gaccgccggc
     2221 acatcggcgg caaacttcct cgaggaatgg ctcaagagcg tagcgggcaa agttgcgccg
     2281 aagacgctca aaaacgacac gatggtagcg cgggtacatc tgattcctgc gctcggctcg
     2341 cacaagctcg agaaactgac gcagcatcat gttcaggcga tgctcgacag caagagtgcc
     2401 aattgtcctt cgcctcgctc cgtcgtctat atccgcaata tcctgcgagc ggccctcaat
     2461 gaagcgatgc gtcaagacct ggtgcagcgc aacatcgcca cactggtaca tccaccgcgg
     2521 atcttagagt atgaggccca taacctcacg ccggacgaag caaacaggtt gctcgcagtc
     2581 gtgcgcggcg atcggctgga ggcgctctac ctggtgtccc tgattcttgg acttcgccag
     2641 ggtgaggcgc tcgggttacg ctgggaggat gtcaacctcg ccgcgggcac gatgacgatc
     2701 cgccgtcagc tacaggtgat caatggacag ccgaccttta cggagcccaa gacgcaacga
     2761 agccgccgta ccatcccact tccggcaccg gtgatcgaca tgctgactcg tcaccgaacc
     2821 cgtcagaagg aagatcgcct cgctgctggt ggacgttggc agtcacagtg gggcctggta
     2881 ttcgcggacg aactcggagc accagccagt ctctctgcgc ttcgcaggaa cttccgagac
     2941 gctcggacac gggccgatct cccacatatg aggtggcatg atctccggca cgcaacatca
     3001 tcattcttgg ccgctcgtgg tgtgcatcca atagtcgcac aagcgatcct gggccacgcg
     3061 tcgctggcca cgacgctaag cgtctacacc catgccagag cggatgcaaa cagggaggcg
     3121 accgacctct tgggcgacct cgcacgcgtc ggaacgggca cgaattaacg tgttggctac
     3181 acgttttggc tacacgagga aggaaaacgt tggtatgaca gggaaattaa tcggtgtacc
     3241 gctattacag gttaggcgct ccacttggtg gagggcttac cgattgtatc tggcgtattg
     3301 cgctggtgaa gtcgatacag caaaagcatc cgcatgggta agcagtcaaa attgggtcag
     3361 aaggaacata tacgtggggg tatccgccgc tgcgtacctc ccgtagtccg acgattactg
     3421 agcgcgcgcg atatggcagc ggctgccgcg aggagcagga gccgtgtcaa tggccttcgt
     3481 tgtcttcagg ccggatgcga tcacgggggc acgctcgaca gccatgcagc gaggtcattg
     3541 ctcatgagcc tttaagctgg actaaagcgt tgtgtgcctg aaagcccgtg acgaacttca
     3601 acctatactt taattcgcca gcactgaggt agctcctcct gtaactgccg gggtcaaggc
     3661 agtcgccccg ttcaccttct cgcgacctcg cgacggcgta acagttgccg cgtcgcaaat
     3721 gcatagatcg attccgacgt accttgcctc aaaccacacc agttgatccg agcttaacaa
     3781 gtgcccgctc atatcgttcg agacgttgtc gtccggcgac atagatggca tgtcgattct
     3841 cgtctggctc gtagctgcgc acgatggtcg tctcgggttc aagcagtgag tcgattgccc
     3901 ggatagattc cagggcagag attgcggcac ctcgggcgga cgcttccgct tcagcgtcca
     3961 gcgcatggat ttggtggttc agcgagtcgg acagaatttg cagccaaagc ggtgaccgga
     4021 gtgccgcacc cccgttggcg tgaatgtcat ggtccgctag ggccagcgcc cttagatcct
     4081 cataaatcga agcgaaccgg taggcggtgg cttcgagaaa ggcacggaac agctctcctg
     4141 ctgtcgtgcc aagactcagc ccggagatag ttccggtcag ctgatcattc caggaaggag
     4201 atcgttcacc cgcgaagaag gggagcacgg taagaccatg cccatccgct tcaatcgccg
     4261 aggcagcctc agttagcgcg tcgaaatcgt ggtcctgggt aagatctgcc agccaatacg
     4321 tgatattgcc accgttgctc agggccccgc ccgcgactcg atgccgccga tccagcctgt
     4381 attgccaaag tttgggggaa agcggttgag agaccggttc atggtcggtc tcgtccccaa
     4441 tgatcacccg catcgcgccc gatgtcccga tggtgagagc aatgcggtcc gcgccaacac
     4501 atcccgcacc gacattcgcc gcggcgccgt cgccgattgc cgggtgccag gcggcgttcg
     4561 cgagatccgg ccagcgaacg cggaattcct ctatcggggg cggaaaggcg acatcgcggt
     4621 cagtcagttt tggcaactgc tcaatggtaa tctcgagcac ggtgagtagt tcatcatgcc
     4681 aatgcgcgcc gcttgaatgg agaagccctg tgccagacgc catggagatc gaggtagaaa
     4741 gctcgtggtg gattgtccag gatagataat cgacgaagga gacccaggtt tttgtcgagg
     4801 cgtaggcgtc agggtctgtg cggcggagcc aacggagctt ggccggccaa taactcgaat
     4861 gaagcctgca tcccgtttca gtgtggatgc ggtccgcgtc cagtgcccgc tcgagggcta
     4921 acgcatcatc accggatcgc ttgtccgccc acataaggac atgcgtcgcg ggctcccgat
     4981 cctcgtccaa tcccatgagg ctatgccaga aggacgtcag gccaacagcg cgaatatcgc
     5041 ctcgccgtat gcccgtattt gcaagggcat gatcgatgca ggagtgcacc aatgagacaa
     5101 gatgatcggg atcggcagtc gagccaccat caggagatgt ttccagcgcg tgccggatct
     5161 ggtgctcggt tcctgtcacc acgcggcccg ctgtatcgta gagaagcgct cgtacagacg
     5221 acgagccgat atcaacggcc atgaccaacg gggtcacggc atcttcgagg tcaatttcag
     5281 gcattctgcg tcccttccgc tcggtccccg ctattatgaa gcatatggtt atatgctcgc
     5341 gcgacttacc tgaagcgcgg cgcggcatgt catttgggtg gcggacaccg attcactcga
     5401 tagcgacagg atcctgaaac tacatggggg acgttgaatc gacgggacgg gcggaaaagg
     5461 agagcgtcag tatcctgcca atagacggca tcgggaatgt gggtccaggg gatccactcg
     5521 agcagatgat cggtgacgca atcgcggaat cggtcggtga gctgcgatgg ggtgatgtcg
     5581 tcgtcgtgac ccacaagatc gtcagcaagg ctgagggcca gctggtcgac ttgggaaccg
     5641 tggagccgtc gcccttcgcg ctagactatg cagccacctg gggcaaggat cccaggcagg
     5701 tcgagatggt gcttcgccag tcgaagcgca ttatccgcat gcaccgtgga atcatcattg
     5761 cagagacaga acacggtttc gtgtgtgcca atgctggtgt tgatgcgtca aatgctgggc
     5821 aggacatggt gtgccttctc cccagggacc cggatgcatc ggcagcgcga attcttgggg
     5881 cgttacaggg acggtttttt cctggtgctg ccgcggagga ccaaccaatc ggcgtgatca
     5941 tcactgactc ctttggaagg ccatggcgga acgggatcgt caacgtggca atcgggatcg
     6001 ccggcatatc gccgcttgcc gattatcgtg gccagttcga tcctgacgga tacgagctgc
     6061 gggcaagcat cttggcagtg ggagatgagc ttgcggccgc agctgagctg gtaatgcaca
     6121 agattgccat gcggccggtg gccgtcatcc gcggctacga acgccagccc ggaccgccat
     6181 ctggtacggg gcaagacctc gtgatgcctg aggaacgcaa tctttttcca tagcgaatca
     6241 cgcggtccgc aaggacgcga acgaagggac cattatgagt aacctgggtt atgccgcgtc
     6301 gttcgagcag tttcacccga atgatcttct ggagtactgc aagcaggcgg agaaagtcgg
     6361 attcggcgtc gtcaccgcca gcgatcattt tcatccctgg gtgccgtcac aggggcagtc
     6421 agcctttgtc tggtcctgga tgggcgcctt gggacaggcg acgtcgctcc ggtttggaac
     6481 gggcgtcacc cctcctggct ggcgctacca cccggcgatc attgcccagg cggcggcaac
     6541 actcgaggcc atgtatccgg gacgattctt cctcgggctt ggcgccggag aagcgctcaa
     6601 cgagcatatc gttggcgact actggccgga ggcacctaca cgactggaga gactggccga
     6661 agcaatcgag atcatcgaga agctgttcac cggcaaggtc atcaagcacc agggcaagca
     6721 tttccggctg gagagcgcga ggttgtatac cttgccggag aatccgccgc cgatttacgt
     6781 ggctacctcg ggtcccatca tggcgggacg cacgggcaag ctcaccgacg ggatcatcac
     6841 ggttggcgcg gcagatgaaa agctgaagat gctgatggaa cgattcgaga agggtgctcg
     6901 cgaagccggc aaggaccctt cctcgatgcc ccggatcctc caggtcaagg tgagctacgc
     6961 gaagaccgat gaggaagcga tcgactcagc agtgagggac tggccgaacg gcggcatgaa
     7021 cttccccaag gcggacatcc gcaatccgga ggacttcgag gcaatggcga agcttgtccg
     7081 accagagaat ttcaagaatc gggtgctcgc aaccgcggat ctcgacaagc atgtggagta
     7141 tatccagcat tttatcgatc tcgggttcgg cgaaatccac gtccacaacg tcaaccggga
     7201 ccaggagcag tttatcgagg cgtatggcaa gaatgtgctg ccaaagctga gctggtagga
     7261 ctccccgagc ctgacgcaag tggggggatg ctgcagaccc acccctccac tgcattgatc
     7321 gaggttccgg attgatggtc tgttcccgag agtctgccaa tggttcctgt tggacgggct
     7381 gaggggcacg atagttgact gatcctcgat gcctcggatg agccggggat ctgatctagt
     7441 gaagtgtgtc gtttgacccg accgtacctg tcgagtacta cgacggccac aagcgggcac
     7501 ctgctttacc ggttggaacg gatatcattt cgcgcggatt gccggatgac tgcgggcggc
     7561 atgagcctat gtgcggtctt gagtaccgct tctccatgct cgacggcttc ttcatacgtc
     7621 atttgggaga ggacgatcga ttggatgatg ccgttctcgt cgataaagac gtgcgtaggg
     7681 aaattggcga tttgccagtc cctggtcatc agggcgagtt gctgggccag cgctggctgc
     7741 tctgtcgagt cgatcatgga cgcgatccgg gtcgggtcaa cgtagactgg gaaggtcgcc
     7801 cccgcagaat cacggtagcg aacggcgtcc tccggggatt cctgcattgc aatggagagt
     7861 acatgcaggc cctgcggtgc aagcgcgttg tacgccttct ggacgtcggg catctcggct
     7921 cggcaaggag ggcaccagga accccagaag ttgatccaga ccggttcacc gcgcaactgg
     7981 gagagcaaga ccggttcgcc agtggaactc atcgtgaaaa gctcaggtgc tggttttccg
     8041 acctcgggga gaagggtggc attgatgccc cctcggccaa tttctgacca gccctgacgt
     8101 tcccccacaa gccacgcgga accgaaaatc gcaaaggcga cgacaagcgc aagggcgcct
     8161 cgcatccagc cctgcgtcgt tccggtgttc gggggtggtg cctgccgcgt gccgtccggg
     8221 tcctggagcg atgcggcgga tttcgctgag ggggtaggta cgtggtctgt catcgtgttc
     8281 tagacaccac cgtaggcgtg caggccgccg aagaaatagt tgccgtagta ggtgaagatc
     8341 gtcgcggcaa agccgaagag cagcacgtac gcgctacgag tgccacgcca tccacggaga
     8401 gacctggtgt gcaggtagac gccatagaca agccaggtga agagggcgga tgtctccttc
     8461 ggatcccact gccagtagac tccccaggcc tcgtgggccc aaaccgagcc caggatcagg
     8521 ttgatcgtga agagggggaa accgatggtg atggctcgga aggcgatgtc gtccagcatc
     8581 tccgggctcg ggagccaggg ggtagaccac cttgtcgcga tcaggaagag tgtcgcggcc
     8641 ccaaaggcaa ccgtgaacgt ggcataggag agcaccgccg cgccgacgtg aaatgtcagg
     8701 atcaggttgg actgcagtgc ggggatgagc ggattgacct cgcgcatggc tggatcgagt
     8761 gaccagatgt agagcgtcat tgcggcggca acactggtgg taatggcgcc gagctgccgc
     8821 accttgtaca tccgctcgaa gacaaggtgc aggatcgata ggaaagagac gaagacgagc
     8881 gagaattcgt acatattcgt caatggaaag tgccctgcgg caccccaccg gagcacaacg
     8941 gccaaggtcg agaaaagagc accaaaaacg gtcaacatcg taccccagcg ggcgaagccg
     9001 atggggccag cctcgtggtg gacgctgttg ctgtcgacac tgtggccggt cgacgtagcc
     9061 atcgtttgct tgaggacccg cacccggccg acagcgaaga cgatgtacat gacggcagcg
     9121 gcgatcagcg agaaggaact cgcggcgaag cagtagaacg agagacggac gagactgtcc
     9181 atagatgctg aaccccttga gcggcggccg attgttcggc cgctgatgtt tgaacctagt
     9241 ctaccgggcc agcacgagtc aggcgttctg gcgtcgcagg tactcatagt ccccggcatt
     9301 cgccgggtgc ttgacgtcgg gtttgacgcc aatcacgtcg tcagccttct ccaggaactt
     9361 gaagaagtcg cgtttaccgc tccagtcgcg tttggccagg ggcgccatgg tcaggaccgc
     9421 gccgtcgacg cccggagaaa tgattccccg tacccgacgg tgcgggaaat agaaggtgaa
     9481 ggcgagcccg ctcaatagtg caacggacgc aatgataaag atcggtatac cggggttata
     9541 gcccacttgc aggacggtga aacgtgtctc gcggtcgaag gtcaccgaga gatcgccgat
     9601 ctttgcgggc ttgccctggg tgaggaccac gccgccctgc ccgatgtcgt tacccgtagc
     9661 ggagccgtca atgggctgca ctcccatcca caactgtcca ctttccaacg ccatggcgtc
     9721 gagctccggc cggttggtcg ggttggagtc gggcgcgacg agagtaagct ggagaccttc
     9781 gctcggcagg cggataaggc ccgctggagc atcgggattg cctcgcatgg tgaagatgcc
     9841 gagttccgcc gcgtccctgt agacgatatt gccgtcgccg tcggtaatcg tcatctgggc
     9901 cgcgttgccg aaactcgact ggtaaaatgt cgcattcctg tagctcatcg ggtgattgac
     9961 cgtgacatcg ccacttttca ccgcctgacc atcctcgtag acggtgatct cgctgcgata
    10021 ctccatcggt atgccgacct ctgtccagga atcctgaaac cggttgagct cgacgcttaa
    10081 tccggtgccg tgaccaacgt tccgtcgcga tccctcagga atgatgaact cggtctcacg
    10141 gaatccatag acggatgtca cgatgccacc aacgagcaac aggatgaggc cgagatggaa
    10201 cgggaatgtg ccgagcttgg acagccggtt cttgtcggca taaaaatggg ttctgtctcc
    10261 caccttctcg gtcaggacgc gatagcgttg agttcgcaac agtgcggaag tagtcgccat
    10321 caggtcgtcc acgctggaag ctgaggtgaa ggttgccgat gtctccgcac cggcgaggaa
    10381 accgggagtc gtggtgatct tcgggtgggt gatcgtcgcc catatgccag ggatgcggtt
    10441 gatcgtgcag acgatgatcg cgaccgtcag gagagtgaga attccggcga agaggctcga
    10501 gcggaagaca tcccacgcat accagcgatc gacaaacccc tccagcggcg ggataaggtc
    10561 cgcgatagct cgcggtacgg agctgccacg aagggttccg ataaggacga ggagggtgag
    10621 aaaggcaatc tccgcgatcg ccgcccggac cgagcagaag aagcgccaga tccggtccac
    10681 aaggacctca agcatcggcc gtgagtgttt cggttgtgag cgagcggtaa cgtcggccat
    10741 gtttcgtccg tttcaggaag aggattcaag gacccaaggc cctagagttc gggctcccaa
    10801 ggcgtccacg gagctacgcg aatgatctcc gtaaaaaggc tctcgtagat gcccagcagc
    10861 atgatcattc ccaccccaag catgaccgcc ccgctggccg ccgtcacgag atgcaggtgt
    10921 ttgttgatac gccgcaggac ctgcggcagg gagccaatcg ccatggccgc cagcaggaat
    10981 ggaactgcca gtccagccgc atagagggtc agcaggacga ctgcccgctt gatgtctccc
    11041 tgtccagctg ccatggtaag gattgcgcca aggatggggc ccatacaggg actccatcct
    11101 gccccaaagc caacgccgat aatgaatgac gatccaaccg taccgggccg accgccgtcg
    11161 agtgacatgt gagcgctccg gtagaggaag gggatccgga atatacctat ctggtgaagg
    11221 ccgagggcca cgagcaagac cccaccgatc cggaccagcc actcccggtt ggtagagaag
    11281 aaatcgagcg aaccggcaaa cgtgccagca gcgccgaagg ccgcgccgaa ggcgatgaaa
    11341 acgagagaga aaccaaccac ataggcgagc gcgttgcgca tcacgacggg ccgcgcggtc
    11401 tgctgcgatt gccccaccga caagccggca atgtgcgtca ggtagatcgg cagaaggggg
    11461 agcacgcagg gtgacgagat cgagaggatc cccgcggcaa atgcagccag aagagagaca
    11521 ttcgtcgtca tcaatgaaac cgtaccatta cctcaatagg ggcgcaattt gttctgctgc
    11581 cggacgggaa cgcgtgcctc gatgtccgct ccacccaaag catagactgt tgttgaggat
    11641 cgagtggcgc ctggaaaggg ggtcctcttg tcgtagctcc tgacgttccc cccgacgtgt
    11701 cgcctgctgt atcgactggt gcctgtgatg actgattcca tgcgcgaaaa gctctatcgc
    11761 tccgaagcca tcgtgctgag ccggatggac tatggggagg ccgaccgcat cctggttatc
    11821 cttacgcccg cccggggcaa gctggatgta attgccaaag gcgcccgcaa agcgcagagt
    11881 cggctcggcc cccagttaga ctatttcgcc cgggtcaatc tcgagctggc gcgagggcga
    11941 gaactcgatg tcgtccgatc cgtggagtcc atcgaccggt tcgccctcct ccgggaaaac
    12001 cttgaagctt tcgggcacgc cagctatttc gtggaattgg tgagaaacct gacgcaaccg
    12061 cgtgaggagc accgtgagct gtatgacctc ctggcgcggt cgcttgttct cctgaacgaa
    12121 aaggtggatc cgtgggccat cgcgcgccac ttcgagttgg cgctcctcac cgcactgggc
    12181 taccgacctg agctgtttca ttgtgtcaac tgcaggcagc ccatccagcc cgaggtgaac
    12241 gcgttctctg cagagcttgg cgggctgctg tgtccgcgat gcagaaatga agatctcgca
    12301 agcatccccc tctcactcaa cgcgcagaag tatctgcgcc tgctggagcg ggggggcctg
    12361 gcagcggcgg cacgtatcaa gcccggtccc gctgacaaga ttgagattca gcaggttacc
    12421 agccattacg ttcggcacgt cgcagaacgg gatttccaca gcctgcgggt gcttggtgcc
    12481 ttgcagcaag cgacgctgga actggcaccg ggatccggtt aatcgctgga tgtatccccg
    12541 aggagatccg gccgccaacg atgcgtacga atctttgcct gctcctgccg ccactggtcg
    12601 atccgcgcat gattaccgct gagcagcaca tcgggcaccg ggatgttgcg atactcagcc
    12661 ggccgcgtgt aatgcggata ttcaacacct gtcaccccgg aaccagcatg ggattcatcg
    12721 aggacgctcg cctcggtgat cacccccgga acaagacgga tgatggcatc ggcaataacc
    12781 atcgcaggaa gctctcctcc ggtaaggacg tagtccccga cggagatttc gtccgcatgc
    12841 agtatctggc tcacgcgttc atcgataccc tcatagtgac cgcagatgag ggcgatccgt
    12901 ccagcggtgg agagctcttg ggcaattgcc tgtgtgaagc ggcgccctcc ggccgacatg
    12961 atcgcgatgt gagcgtgatc caggtcatcg ccaagaatct cctcaaccgc ttcgatgatc
    13021 ggtggagcct tcatgaccat gccggcacca cccccgtagg gcgtgtcgtc ggctgtgtgg
    13081 tggcgatcgt acgtccagtc ccggatgtcg tgggccttga tctcgatgat gcccttggcc
    13141 tgcgcacgct tgatgatgct ttcgccgaag actccctcga acatgggcgg aaagagcgtg
    13201 aagatatcga tccgaagagc tgggcgaatg atcatgtaag gggcatgtcc agatagcggt
    13261 agatccaggg agcagtctcc gacagggcgt tcgctgtgta gtcaggcgtt gtatcaggag
    13321 cacgatcgac gaacgcgtcc atatccccat tggagaacca gatcgctcgc ataccggcct
    13381 gttgcgctgc ccacacgtcc catttagggg aatcgccgac gtggatgcta caggaagggt
    13441 cggagccgac cagcgacatc gcgtgcctgt agatttcggg gttggatttg tagtgaccac
    13501 aactggccga cgtcacaaca aaggcaagat cccgggcaat gtcgaaatgc tctagcgtcc
    13561 actccaggaa tggatggtag acagcactgg agataacccc caccgggatc cctcgacccg
    13621 cgatctcccg aacaacctca attgcgccgg gcacgggttc aacgtgctcc atcgcctgcc
    13681 gcatgagacg gtgaacgccc tcttcgatgg atgcgcgatc tacctcgata tccaactctg
    13741 cggcgatgcg ggcaacgcaa tcggcggcgt caatctcccg gcccgaggcg attacacctt
    13801 ggcggagaga gcggtatcgg gcggtgatct cttcgggtgt gttggcgtgg agcaacgctg
    13861 gatcaatatg ggacaggaca tcgacgggaa gatcccgaat ctcgagataa aaccagggat
    13921 cgcaggtggc gagcgtgttg tggaagtcaa aagtgacgag atttttcacg ggtgtcaagt
    13981 atccctgtcg ggggttccgc catgcggcag gaaaacgatc tgccaatgat tgtaacggag
    14041 ggcaggtgac gatatacgga tgaagcatga ttatggcctc ggatcttctc ttctcggcaa
    14101 gtcattcctt ggattgccac cttccgggtg aacgaagcgg aacaagtcgc ggggatgcac
    14161 gatcaactct gcgctatccc gtctcccggc ccggactgga ttagttatcg gtatgattgg
    14221 cgtacgagaa agctcggtcg gctctccggg cggggaaagg attttttgtg cccacggcac
    14281 tgactattga tgtcgggttc gcgaagaccg agaaatacgc cagtcgcgag agcggtgaca
    14341 ctgttgagct ggttgagcgg cctggtggtg gcttttcgat cgtgatgatc gatggtcagg
    14401 gctcggggaa ggcggcgaaa accctcagcc tgctgctctc gtcaaaagcg gtgggtctta
    14461 tcaaggaggg cgtccgcgac ggcgtggtcg cgcgggccac gcacgatcat ctcttcgcct
    14521 tccggcacgg tcgggtgtcc gcgacgtttg atttgctgtc ggtggatatg cagactaagt
    14581 cgatagtcgt ttcccgcaat gcggaatcgc cgatgctcat ctgcgatgat ggggggcacc
    14641 gtgttgtgcc ggtggattcg ggacccatgg gccggtatca gtggacgaag ccgacggtgg
    14701 ttgagctgcc gatgaaagcc ggtatgagcg tcgcgctctt cacggacggg attccccact
    14761 ccggccggcg ggcaatgctg cccccgatcg atttcgatgc tctggcagaa gcaactatga
    14821 ggggcggcgg tccagcctct gatgtggcag aagcgatcct gcagaaggcc atcgaacggg
    14881 atcagggacg tcccggcgac gatatggccg tgatcgcact gtcgatcgtg gagcaggaac
    14941 cagatatgct catccggcga atgagcgccc agttcccaat ggcctgacga ttggcgtggc
    15001 cctttgaccg agcgacgagg gaggtgtcga ggtgaaccag gaaactcacc cggtcgatcc
    15061 acgtcgcatc gtggtcgtcg gaccctgcgg cgccgggaaa acgacacttg tggatcacct
    15121 ccgagagcaa ggctatgatg cccgcgttgt cgctcaggag cacagcatca ttcgcgatct
    15181 ctggcggagg cgggaaccgg atgttgtggt ggcactcgat cttgatctgg cgacgcttag
    15241 ggggcgccgt tctcctacct ggtccgctga tatctttgag aaacagcatg atcggctaag
    15301 ggaagcgttc gctgcagccg atatcgtcat cgataccggc agacatggtg aggcggaagt
    15361 tgtcgagatg gtgaaggggt ggctgaacca ccatcccaga tgaggtgcct gtcatcacgt
    15421 catggtattg ccgtcgcggc gaccctcagg attgcggcca attaccggtg ccagggggtc
    15481 agttcaacga gaacctgctc cctggatggt tgacggccag acccgcggcg cccgcatgca
    15541 ggagcagggc cagctcgcgc tcgaccaaca gggaaagctc tctcagtctc tcgacggcag
    15601 tctcggcctc aagcaatcgc tgccgctccc atgtatggag tggcaggcgc gagaccagat
    15661 cgtaggatgc ccggacaggc tcctcgggaa tcatcacgcc ggaaaatcgt cgccttgtga
    15721 gccgggtgat accaccggca taccgatgga actgggctcc gactgtccga agtaaggcgt
    15781 ctgcctctgt cggatcgccc aatatctcct ccagccactc gattgtcgcg atgccatacg
    15841 gttgggattc gtcgaagtcg acgattcgga agcgttgctg accctctacg accagatccc
    15901 accggccatc gggaagctct gtacggccgg taatcattgc cgccgtgccg accgagtgtg
    15961 ccggccatgg ccgatgcggc ggccgctccg tgtggatgag tgcaacaccg aaaggtatgg
    16021 tgtcagtgcc gtgatcgtgc atcagctgga ggtatcgggg ctcaaagata tgcaggggca
    16081 tgggcatgcc cgggaggagg acagttccaa gcgggaagag tgggagcagg gagggcatgg
    16141 gtgtctttcg taggggattc aaggtagcgc gaccgatatc gccagggtcc attgtacggc
    16201 ctgtcccagg cgagaacacc aaattgtaga atcaccagac gctcgccgat tctggtggtc
    16261 gattatcctg attcgcaagc gatgctttgg gtccttttcg ggagtgacac cccgggaatt
    16321 acaccagatg cagtaacgct acggatgaga agctgcctga agagacgaag acagaataag
    16381 gggacgtagc gtgtgagcgt ctcgataagg aggcccagac attgcccatc gaccgacggt
    16441 agtgaccgtg ctccgtccac tcattccata ttcagtcacc tggaaagaga tgccccggga
    16501 ccaatgagcc aaatgggatt gctaagcctg gagtcgttgt catgatgctc aagggaatag
    16561 cgcgaggacg gtctgatcca ggtgccgaca atggcgtcag aacgcttaca ccggcggata
    16621 tcgacctact tgctatccgc aaccacccgc gtctcgaaga gggtgacgcc gaggcattga
    16681 tcatccaggc gcccgggtta tccaagtggc atcccgcgag cagggaattc cttatcgtga
    16741 cgccgtggcg acacagggcg gaaatcccgg ctgtgaatgt gatgtccacg tttcatcacg
    16801 aggatgcatt gatggcggcg gcaatcgcgt cggccgagaa acacggagct gcagcgtttg
    16861 tgctgctgga gacctatgag acacggcaac cgtcgttcta cgcccgaaac gggatgcagc
    16921 gtctcgaaac gatcgttacc tacgagctca cccagcccag gaccttcctc aggcagataa
    16981 cgcagcgccg gcagcagttc gtgaagctgg accggcagac agccgagctg gggcaggctg
    17041 ttctcaatct cgatcacgtc gcctttccct ggctctggtg gaactcggca gaggagttct
    17101 caaactacct ttccatgccg aatatcgagg tgtgggcggg aatcctcgac ggtgagctca
    17161 tttcctatgt cgggtttacg cacttctggc gttggtccca tcttgaccgc atagcaattc
    17221 ggcccgatgc tcagcggcag ggcttcggcc gtgaggcgtt gcattttgcc gtcgaacgga
    17281 tgttggagcg aggcgctacc agggtggggc tgagtacgca gggagagaac acggcgtcgc
    17341 ggaatttgta tgaatccatc gggttccgga aaaccccggg ccatgattac gacgtccatg
    17401 gcgtggtttt cgagccggga cgcaaatatt tagagcaagg gggccagggg tgacctcaca
    17461 ttatgggcag tagcttcggc aagatattca agctgacaac ctggggcgaa tcgcacggcc
    17521 ccgcactcgg tgtcgtcgtc gagggctgtc ctgcaggaat tcccctgacg gtagacgaga
    17581 tccagcatga gcttaatcgc cgccgtgttg gccagagcaa ggtcacgacc ccacgaaacg
    17641 agaaagatca ggcagaactg ctctcggggg tctatgaggg catcacaac
//
LOCUS       DASYWM010000160         9553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_156207, whole genome shotgun sequence.
ACCESSION   DASYWM010000160 DASYWM010000000
VERSION     DASYWM010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156207"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VGR29_06490"
     CDS             complement(<1..1016)
                     /locus_tag="VGR29_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV2073277.1"
                     /translation="MTTASWHPAFGAQMEDGGVRFSVWAPNAETVDLELLENNLSRTV
                     QMTGDADGRFETLVPDIGAGARYLYRLDGGDGRPDPYSRFQPEGVHGPSEVVDPHRFT
                     WTDSEWRGITPEGIVVYELHIGTMTPGGTFLSLIDELAELKSLGVTAIELMPVAQCPG
                     RWNWGYDGVDLFAPSNAYGTPDEFRQLVDAAHGVGLGVILDVVYNHLGPEGNYLGVYA
                     NEYFSTVHETGWGAGLNWDGEKCEFVRQFAIDNACYWITEFHIDGLRLDATHAIVDDC
                     PIHLVQELTAKARDVAGDREIFVVAEDGRHEITRARAIDEGGEGLDAIWADDFHHDVR
                     VHLT"
     gene            complement(1018..4020)
                     /gene="treY"
                     /locus_tag="VGR29_06495"
     CDS             complement(1018..4020)
                     /gene="treY"
                     /locus_tag="VGR29_06495"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEV2073278.1"
                     /translation="MSSRINTSERVRDLVTDAMQARRAIPAPRATYRLQFNHTFTFAD
                     AIKLVPYFADLGISHVYASPIFKAAPGSMHGYDVVDYGEINPEIGTREDFDRFVSELH
                     QHGLGLIIDFVPNHMGIEKGANAWWQDVLENGQMSRYAEFFDIAWSPLKRELQGKVLL
                     PFLGGQYGEVLERGELTLVMEDGGFLIRYFDTPFPIDPRTWPFILRRAQEAVEGLLAP
                     DDLDLLEFESVIAALEHLPVAGVDEPTADAIAARHREHLVACHRLDELGKRCDVINDA
                     ISGVVEALNGEVGDPRSFDALDHLLSMQPYRLSYWRVAAEEINYRRFFAINTLAAIRQ
                     EEPEVFEETHRLLISLLVEGSIDGVRFDHPDGLWNPEGYFRAVQEATIREEVRRRLSP
                     ETDDEWEWLKPDLDLAIDEALNQIRESDRHWPVYVVGEKILEHGEALPTGWEIAGTVG
                     YEFAQAATGLFVDPEARALFDKVYTRFTGDNIRFPELVYEMKQRMMREAFPSEVNVLT
                     NVLNRISERDRHSRDFTVNNLRAALREVMACFSVYRTYTTCDAFGVADRDRKYIETAV
                     AQAKKRNPGTDVSVFDFVQEVLLLHVTDNPRERYDQRCHFAMKVQQLTGPVMAKGLED
                     TAFYRFNRLTSLNEVGGDPAKFGTSVDEFHRQNRARRRDWPESMINSSTHDTKRSEDV
                     RARVSVISEKPTEWRAALNRWSRINRKLKTRVEGALAPHRVDEYMIYQTLIGSWPLGG
                     LESAHDSYVERIQEYIIKVAREASRFTNWVNPNEGYEEAARAFVSGMLDARRSAAFLD
                     DFRAFVEARIDPGLINGLSQQVLKLTSPGVPDIYQGTEFWDDSLVDPDNRRPVDFDAR
                     TEALAAQESVSLEDLVAERTDGRIKLAVTSRLLAIRREHKALFAQGEYVPVHPEGPAA
                     EHLVAFLRKHQGIELLVVVPRLLMKLAGSGGVLDSEIWSGTTIPYAGEDAWRDVLDDR
                     QFEAGDLAVSGLFETLPVSVLIRGEEER"
     gene            complement(4057..5511)
                     /gene="argH"
                     /locus_tag="VGR29_06500"
     CDS             complement(4057..5511)
                     /gene="argH"
                     /locus_tag="VGR29_06500"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV2073279.1"
                     /translation="MAEHSGAAKAWGGRFAESPDARLEAFNASVGFDVRMIREDIRGS
                     IAHVRMLGRQNIVPVDEADSIEGALWQVLGEVEAGKFLLTIADEDVHTGVERRLRELV
                     GGIAGKLHTGRSRNDQVATDFRLWTKRMLVEIATGLVRFGEALLTVARENADVIMPGY
                     THLQRAQPILLAHHMHAYGEMALRDLDRVREAFRRTDVLPLGSAALAGATYPLDRESV
                     AADLGFARISANSLDAVSDRDFALDALYACSLISLHVSRLSEELILWSSGEFRYIEIA
                     DRFSTGSSIMPQKKNADIAELGRGKTGRIFGDLLALLTTMKGLPLAYNKDMQEDKEGL
                     IDAVDTILAVLDVFPPMLESLTVHGDCTARAAIEDFTLATDAADMLAKAGVPFREAHE
                     IVGSLVGRCIAEGKTFADLTDEEWAAVHPVFANERPPLDGAGSVALRDVPGGTAPIRV
                     AVAGKRLEGNIANHREWVVHQDQVLEALFAPPSS"
     gene            5715..6824
                     /locus_tag="VGR29_06505"
     CDS             5715..6824
                     /locus_tag="VGR29_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073280.1"
                     /translation="MTMATTQTSRQTLPDLVRGIEERLQGQGVALTDMRRSGSLETDV
                     NVPQILDGSSTETRSAQVERLEGPIVARPIERRNETNFAWFLDGSQKTLPVWRIGVVP
                     VIVGIAVVGILERDEEGACQLLGDTLVERLTWFVPMQTQSVEMNRLIDILQEAGQEIR
                     DPLEKFARNAPDLGQYHALAGNYGHLLYYAQQQAGRVRGAVEQELVRYWDSRIRNPDL
                     DKWLVVDGRLQGQYPNAIGFVKDPTSQHLFGEEATALYSLPQGHRTSAYQLRPSGGEE
                     EPESELRNQILTMWYQRMWASTGLDARHALVRLEASSEVWEPELIDNIAAWLMAERIP
                     RPTKDSRWPTLLYPVHLLELMLKRRINAMTVGWPS"
     gene            6845..8947
                     /locus_tag="VGR29_06510"
     CDS             6845..8947
                     /locus_tag="VGR29_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073281.1"
                     /translation="MALDFDITTQQIWEQDRFTDLVDHKTGAVGRVVSSEREPAGSHQ
                     FYFWASDVALTLDVGHIVVTFSEEAAVIGVVDEPRRYSDLVSFLDDYFDRRMELALPE
                     EGPTMRPEILVFTVKVLATKHVRDDVSSNRPAINGPVFFATEAAIEYALGVHQFSGTP
                     IPALMHTNGNYVRDADGDVLTDMHGLERFQKTPLFLDEEYLLGPEAGHANWTGQSGLA
                     TKTSHALFLTSSVFQTMAREGKTVAALMFNVKGPDLLWLDKPAQPEPAMEDAYAASGW
                     KGLDKDQLSAYESLGLDPRPFENVNIFAPFRPGMTPQSANKRVDLSGFHDYKSLNTER
                     NIPGESDRVFPILWSLDRALWYPHRIFDFNDLDDKLFGFIYELRERKIGSLDELRELF
                     SRIENELAESGQDTWNGHHKATIRKAQNRFKGLQDKLGGLLADGAVHYGATPQVDYGF
                     NPGELRVVDISACNTNAQELIVSSTINAIWKLAEKSDMGVDKLIVFVDELNKYAPAGG
                     EGGLRDTLVDIAARGRHLNVVLLGAQQFRSKVDTEILGNCGTSFYGRIGDEEIINAAY
                     RSISDTSKQELLGLAKGRLLVRHAHFRAPLFGTFPLPPTIPGVNGQRVFGKEKASQPN
                     VHPSDPIFRLLQNLLGDNAPRKNEVMIACDGLDGEDIEQVRSNVNRMFREGSSPRGNA
                     WRATQKQLLKARELKSMS"
     gene            8944..>9553
                     /locus_tag="VGR29_06515"
     CDS             8944..>9553
                     /locus_tag="VGR29_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV2073282.1"
                     /translation="MSESIRIAHITDTHLGFRALSKSDPETGRNQRTVDFEKALERAI
                     DDILTRDVDAIIHAGDVFHHTRPTWQTLRHFIQQFRRLEKAGIPTLVIAGNHDTPRIR
                     TGGSAYSVLELALPEIRFVAEYEDIHEVDLFPDLNLHVHAIPHGALTNDDPVMPYLLS
                     GKRNVLACHGMVPGILDPGVSAEPGEQVLNTSLLTPDFDYIAL"
BASE COUNT         1911 a         3120 c         2745 g         1777 t
ORIGIN      
        1 ttcgtcaggt gaacacgaac gtcatgatgg aagtcgtcgg cccagatcgc gtccagtccc
       61 tcaccgccct catcgattgc acgggctcgg gtgatctcat gcctgccgtc ctcggcgaca
      121 acgaagatct cccggtcacc ggctacgtct cgagccttgg cggtcagctc ctgcacgaga
      181 tggatcgggc agtcatcgac gattgcgtgg gtcgcatcga gccgtagccc gtcaatatgg
      241 aactccgtga tccagtagca cgcgttatcg atcgcgaact gccggacgaa ctcgcacttc
      301 tcaccatccc agttgagccc cgctccccag ccggtctcat ggacggtgct gaagtactcg
      361 ttggcataga cgccgagata gttcccctcg ggaccgaggt ggttgtaaac cacatcgagg
      421 atcaccccga gtccgacccc gtgggcggca tcaacgagct ggcgaaactc atccggagtg
      481 ccataggcgt tcgacggcgc aaacagatcg accccgtcgt agccccagtt ccaccgtccc
      541 gggcattgcg ctacgggcat caattcaatc gccgtaacgc ccaggctctt cagctcggcc
      601 aactcgtcga taagcgagag aaacgtgcct cccggcgtca tcgtcccgat gtgaagctcg
      661 tagacgacaa ttccttcggg ggtgatccct ctccattcgc tatcggtcca agtaaatcga
      721 tgcggatcaa cgacctccga gggcccatgt acaccttccg gctggaagcg ggagtatggg
      781 tccggcctgc catctccgcc gtccaaccgg tacaagtagc gagccccagc tccgatatcc
      841 ggcaccaacg tttcgaaccg accgtcagcg tcacccgtca tctgcacggt gcgggagagg
      901 ttgttctcca gcagctccag atcaaccgtc tcggcattcg gtgcccacac ggagaagcga
      961 acgcctccgt cttccatctg cgccccgaat gcgggatgcc agctcgctgt tgtcatctca
     1021 gcgctcctcc tcgccccgaa tcaggacact tacgggcaat gtctcaaaca acccgctcac
     1081 ggcgagatcg cctgcctcga actgccggtc gtccagcacg tcccgccatg catcttcccc
     1141 ggcatagggg atcgttgtcc cgctccagat ctccgaatcg agcaccccgc cggaaccggc
     1201 cagcttcatc agcaggcgtg gcacaaccac cagcaactcg atgccctggt gctttcgaag
     1261 gaatgcgaca agatgctcgg cggccgggcc ctcgggatga accggcacgt actcgccttg
     1321 cgcgaagagc gccttgtgct cccgcctgat tgccagaagg cgagacgtca cggcgagctt
     1381 gatccggcca tcggtccgct ccgccacgag atcttccagc gaaacgctct cctgcgcggc
     1441 gagcgcctcc gtgcgggcgt cgaagtcgac agggcggcga ttgtcggggt ccaccaggct
     1501 gtcatcccaa aattccgtgc cctgatagat gtccggcact cccggcgatg tgagcttcag
     1561 gacctgctgg gagagtccgt tgatcaggcc gggatcaatt ctcgcttcga cgaaggcacg
     1621 aaagtcatca aggaatgcag cggatcgcct ggcatccagc atcccggaaa caaaggcgcg
     1681 cgcggcttcc tcgtacccct cgttcggatt cacccagttc gtgaaccggc tggcctccct
     1741 ggcgaccttg atgatgtact cctgtatccg ctccacgtag ctgtcatgcg ccgattccaa
     1801 gccgccaagc ggccacgagc cgatcagcgt ctggtagatc atgtattcat cgacgcgatg
     1861 tggcgcgagc gctccctcga ccctggtctt gagcttccgg ttaatgcggc tccagcggtt
     1921 cagcgccgct ctccattcag tcggcttctc gctgatcaca ctgacccggg cccgcacatc
     1981 ctccgaccgc ttggtgtcgt gcgtcgacga gttgatcatc gactccggcc agtctcgccg
     2041 ccgcgccctg ttctgccggt ggaactcgtc cacgcttgtg ccgaacttcg ccgggtcacc
     2101 gccaacctcg ttgagcgacg tcagacggtt gaagcggtag aaggccgtgt cctcaagacc
     2161 cttggccatc acgggccctg tcaactgctg gaccttcatc gcgaagtgac accgctggtc
     2221 gtagcgttcc cgcgggttgt cggtcacatg caacagcaac acttcctgca cgaaatcgaa
     2281 gaccgataca tccgttccgg gattgcgctt ctttgcctgg gcgacggcgg tctcgatgta
     2341 cttcctgtcc cggtccgcca ccccgaacgc atcacacgtc gtgtaggtgc gatagaccga
     2401 gaagcaggcc atcacctcgc ggagcgccgc gcgcaggttg ttcaccgtga agtcccgcga
     2461 atgccgatcg cgctccgaaa tgcggttcag cacattggtc aggacgttga cctcactggg
     2521 gaacgcttcc cgcatcatcc gctgcttcat ctcgtagacg agctccggga agcggatgtt
     2581 gtccccggtg aaccgggtat agaccttgtc gaacagggct cgcgcctcgg gatcgacaaa
     2641 gagtccggtc gcggcctggg caaattcgta gcccaccgtt ccggcgatct cccagcccgt
     2701 gggcagagct tctccatgct cgaggatctt ttcccccacc acgtagacgg gccaatgtcg
     2761 gtccgactcg cggatctggt tcagtgcctc atcaattgcg agatcgagat ccggcttcag
     2821 ccactcccac tcatcgtccg tctccgggga cagccgccgg cggacctcct cgcgaatcgt
     2881 ggcttcctgt accgcccgga agtacccctc cgggttccac aagccatccg ggtgatcgaa
     2941 gcgcacaccg tcgatcgacc cctcgacaag aagggagatg agcagccggt gcgtctcctc
     3001 gaacacctcc ggctcctcct gacggatcgc cgccagagtg ttgatcgcga agaaccggcg
     3061 gtagttgatc tcctcggccg ccacccgcca gtaggacagg cgatagggtt gcatgctcag
     3121 caggtgatcg agcgcatcga aggaacgggg atcgcccact tcgccgttca gtgcctcaac
     3181 gaccccgctg atggcatcgt tgatcacgtc gcaccgcttg cccagctcat ccagccggtg
     3241 gcaggccacc agatgctcgc gatgacgcgc cgcgatagcg tcggcggtgg gctcatccac
     3301 gccggcaacg ggaagatgct ccagcgcagc gatcacgctc tcgaactcga gcaagtcgag
     3361 atcatcggga gcaagtaacc cctccactgc ttcctgggcc cggcgaagga taaagggcca
     3421 cgtgcgggga tcgatcggga acggcgtgtc gaagtaacgg atcaggaagc cgccatcttc
     3481 catcacaagc gtcagctcgc cgcgctccag cacctctcca tactggccac cgaggaacgg
     3541 cagcagcacc ttgccctgca actctcgctt caacgggctc caggcgatat cgaagaattc
     3601 ggcgtagcgc gacatctgcc cgttctcgag aacgtcctgc caccaggcgt tggcaccctt
     3661 ctcgataccc atgtggttgg gaacgaagtc gatgatcagg cctaggccat gctgatgcag
     3721 ctcggagacg aagcggtcga agtcctcacg ggtgccgatc tcgggattaa tctccccgta
     3781 gtcgacgacg tcgtacccgt gcatcgaccc gggtgccgcc ttgaagatcg gcgaggcgta
     3841 gacgtggctg ataccgaggt cggcgaagta cggcaccagc tttatggcat cggcaaacgt
     3901 aaaggtgtga ttgaactgga ggcggtacgt cgcgcgtggc gccgggattg cccgccgcgc
     3961 ttgcatggca tcggtcacga gatcacgcac gcgctcggat gtattaatcc tggaactcaa
     4021 gttgttctcc aaagcatggc aacaggaatg tcaatatcaa ctggacggcg gtgcaaacag
     4081 tgcctcgagc acctggtcct ggtggaccac ccactcccga tgattggcga tattgccctc
     4141 aagacgcttc cccgccacgg caacccggat gggcgccgtg ccaccaggga cgtctctcag
     4201 agcgacgctc cccgccccat cgagaggtgg tcgttcatta gcgaacacgg ggtgaacagc
     4261 tgcccactct tcatcggtga ggtcggcaaa cgtcttgccc tcggcgatac atcggccgac
     4321 aagcgacccg acgatctcgt gcgcctcacg aaatggcact ccggccttgg ccagcatatc
     4381 cgccgcgtcg gtcgcgagcg tgaaatcctc gatggccgcc ctggctgtgc aatcaccatg
     4441 cactgtcagg gactcgagca tcggtggaaa aacatccagc acggcaagga tggtatcgac
     4501 cgcgtcgatc aacccttcct tgtcctcctg catgtccttg ttataggcca gcggaagacc
     4561 cttcatcgtc gtcaataacg caagaaggtc gccaaagatg cggccggtct tgcctcgtcc
     4621 aagctcggcg atgtctgcat tctttttctg cggcatgatc gagctgccag tggagaagcg
     4681 gtcggcgatc tcgatatagc gaaactcgcc ggaggaccag aggatcagtt cctcactgag
     4741 ccgggaaacg tgcagcgaga tcagagagca ggcgtacagg gcatcgaggg cgaaatcacg
     4801 gtcggagacc gcatcgaggc tgttcgccga gatccgggca aagccaagat cagcggctac
     4861 gctttcccga tcgagtggat aggttgcacc ggcaagtgcg gcggagccga gaggcagcac
     4921 atcggtgcgg cggaaagcct cacggacgcg atccaggtcg cgaagggcca tctctccata
     4981 ggcgtgcatg tgatgggcga gcaggatcgg ctgggctcgc tgcaggtgcg tgtagcccgg
     5041 catgatgacg tcggcattct cccgcgcgac ggtcagcagg gcctcgccaa aacgcacaag
     5101 accggttgcg atctcaacca gcatgcgctt cgtccacaac cggaagtcgg ttgctacctg
     5161 atcgttgcga gagcgtccgg tgtgcaactt cccggcaatg ccaccgacaa gctcgcggag
     5221 ccgacgctcg acaccggtgt ggacatcctc atcggcgatc gtcagcaaga acttgcccgc
     5281 ctcaacctct cccaggacct gccacagtgc cccctcgatc gagtccgctt catcaacagg
     5341 gacgatgttc tggcgaccaa gcatccggac atgagcgatc gagccacgga tatcctcccg
     5401 gatcattcgc acatcgaatc ccaccgacgc attgaatgcc tccagccggg catcgggcga
     5461 ttctgcaaac cgtccacccc atgccttggc tgctcctgaa tgctctgcca tcttcccgcc
     5521 cccgcactgc acgtacaccc ctcggcgtgg caaaattatc caagcgccac ctgttagcct
     5581 accatcagcg tagataagac accgtaggac aaaacatggg gtatactcag gaatagcaca
     5641 cctgttcgtg tatacgtaca tcggcatccc cgccgccgat atcttcgaac gccccgggaa
     5701 agcagagcct cgacgtgacg atggcgacaa cccaaacaag caggcagacg cttccggatc
     5761 tcgtcagggg catcgaggag cgcctgcagg gtcagggcgt tgccctgacc gacatgcgtc
     5821 gctcgggctc gctcgagacg gatgtgaatg ttccgcagat actggatggc tcctccacgg
     5881 aaacgcggtc ggcccaggtt gaacgcctcg aaggcccgat tgtcgcccgg cctattgagc
     5941 gacgaaacga gacgaacttc gcctggttcc tggatggcag ccaaaagacg ctcccggttt
     6001 ggcgcatcgg ggtggttcca gtgattgtgg gaatcgcggt cgtcggcatc ctcgagcggg
     6061 atgaggaggg agcgtgtcag ctgctgggcg atacgctcgt ggagcgactg acgtggtttg
     6121 tcccgatgca gacgcaatcg gtcgagatga accgcctcat tgatatcctg caagaggctg
     6181 ggcaagagat ccgcgatccg ctggagaagt tcgcccgaaa cgcccctgat ctcggtcagt
     6241 accatgccct ggccggcaac tacggccatc tgctctatta cgcccagcaa caggcgggcc
     6301 gtgttcgcgg agctgtggag caggaacttg tccgctattg ggactcccgc attcggaatc
     6361 cggacctcga caagtggctg gtcgtggacg ggcgactgca ggggcaatac ccgaacgcga
     6421 tcggattcgt gaaggacccg acatcgcagc atcttttcgg tgaggaggca accgcgctct
     6481 actccctgcc gcagggacat cgcacctcgg cctatcagct gaggccctcg ggcggcgaag
     6541 aggagccgga atcggagcta cgcaaccaga tcctcaccat gtggtaccag cggatgtggg
     6601 cctcgaccgg gctcgatgcc cgtcatgccc tggttcgtct tgaagccagc agcgaagtgt
     6661 gggaaccgga gcttatcgac aacatcgccg cctggctcat ggccgaacga attccccgcc
     6721 caaccaaaga ctcgcgatgg ccgaccctgc tctatcccgt ccacctgctc gagctcatgc
     6781 tcaagcgccg tatcaatgca atgactgtcg gctggccctc ctgacctcac gacccggagt
     6841 tctcatggca ctcgatttcg atatcacgac acagcagatt tgggagcagg accgcttcac
     6901 cgatctcgtc gaccacaaga caggcgcggt cgggcgggtt gtcagcagcg agcgtgaacc
     6961 ggcaggatct caccagttct acttctgggc ctccgatgtg gcactcacgc tcgacgttgg
     7021 ccatattgtc gtcaccttct ccgaggaggc cgccgtcatc ggtgttgtcg acgagccgcg
     7081 ccgatattcc gacctggtct ccttcttgga cgactatttc gaccgacgta tggagctggc
     7141 acttccggaa gaaggcccga ccatgaggcc ggagatccta gtcttcacgg tcaaggtgct
     7201 ggccacgaag catgttcgcg acgatgtttc ctcgaaccgg cccgccatca acggcccggt
     7261 ctttttcgcc accgaagccg caatcgaata cgcgctgggt gttcaccagt tcagcggcac
     7321 gccgatcccg gcgctgatgc ataccaacgg gaactatgtc cgggatgctg acggtgatgt
     7381 cctcaccgat atgcatggcc tggagcgctt ccaaaagacc ccgctcttcc tcgacgagga
     7441 atatttgctc ggcccggaag ctgggcacgc caactggacc ggccaatccg gcctagcgac
     7501 gaagacaagc cacgcgctct tcctgaccag ctccgtcttc cagaccatgg cgcgcgaggg
     7561 caaaaccgtc gccgccctca tgttcaatgt gaaaggaccg gacctgctct ggctcgacaa
     7621 gccggcgcag cctgaaccgg ccatggagga cgcctatgct gcctcgggct ggaagggatt
     7681 ggacaaggac cagctcagtg cctacgaatc ccttgggcta gatcccaggc cattcgagaa
     7741 cgtcaacatc ttcgcgccct tccggcctgg catgacaccc cagtccgcca acaagcgcgt
     7801 cgacctcagt ggctttcacg actacaagtc gctcaacacc gaacgcaaca tacctggaga
     7861 atcggaccgg gtcttcccga tcctctggtc gctggaccgg gccctctggt atccgcaccg
     7921 gatcttcgat ttcaacgacc tcgacgacaa gctcttcggc ttcatctatg agctccgcga
     7981 gcggaagatc ggttccctgg acgagcttcg ggagctcttt tcgcgtatcg agaatgagct
     8041 ggccgaatct ggacaggaca cgtggaacgg ccaccacaag gcaacaatcc gcaaggcaca
     8101 aaaccgtttc aagggcctgc aggacaagct cggtggcctg ctcgccgatg gcgcggtgca
     8161 ctacggtgcc acaccgcagg ttgactatgg gttcaatccg ggagagcttc gcgtcgttga
     8221 catcagcgcc tgcaacacca atgcacagga gttgattgtc tcctccacca tcaacgcgat
     8281 ctggaagctt gctgaaaaga gcgatatggg cgtcgacaag ctgatcgtct ttgtcgacga
     8341 gctgaacaag tacgccccgg cgggaggcga aggcggcctg cgggacaccc tcgtcgacat
     8401 cgcggcgcgg ggtcgccacc tcaacgtggt acttctcggc gcccaacagt tccgctcaaa
     8461 ggtcgacacg gagattctcg gcaactgcgg cacgtcgttc tacggacgta tcggcgatga
     8521 ggagatcatt aacgccgcct accgttctat ctccgacacc tccaagcagg aactgcttgg
     8581 tctcgccaag ggccggctgc ttgtccgcca cgcgcacttc cgggcaccgc tcttcgggac
     8641 cttcccactg ccaccgacca tccctggcgt caacggccag cgcgtctttg gcaaggagaa
     8701 agcttcccag ccaaacgttc atccgtctga tccgatcttc cgcctcttgc agaacctgct
     8761 tggagacaac gccccgcgca agaacgaggt catgatcgcc tgtgacggcc tggacggaga
     8821 agatatcgag caggtccggt ccaacgtcaa ccgcatgttc cgggaaggga gcagcccgcg
     8881 tggcaatgcg tggcgcgcga cccagaagca gctgcttaag gcacgagaac tgaagtcgat
     8941 gtcatgagcg aatcgatccg cattgcccat atcactgata cccatctcgg ctttcgggcg
     9001 ctgagcaaat ccgatccgga gacagggcgc aatcagcgca cggtcgattt cgagaaggca
     9061 cttgagcgcg caatcgatga catcctgacg cgcgatgtag acgccatcat ccatgccggg
     9121 gatgtctttc atcacacgcg cccgacgtgg cagacattgc gccatttcat ccagcagttc
     9181 cgtcggctcg agaaggctgg catccccaca ctcgtcattg ccggcaacca cgacactcct
     9241 cggatccgca ccggaggctc cgcctactcg gtcctggaac tggccctgcc cgagattcga
     9301 ttcgtcgcgg aatacgaaga catccacgag gtcgacctgt tccctgacct caatctccat
     9361 gtgcacgcga tcccccatgg ggcgctcacg aacgacgatc cggtgatgcc ctacctgctc
     9421 agcggcaagc gaaacgtgct cgcctgccac ggcatggtgc caggcattct cgatcccggg
     9481 gtgagcgccg aaccgggaga gcaggtgctc aacacatcct tgctcacgcc tgatttcgat
     9541 tacatcgcac tcg
//
LOCUS       DASYWM010000161         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_401016, whole genome shotgun sequence.
ACCESSION   DASYWM010000161 DASYWM010000000
VERSION     DASYWM010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401016"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VGR29_06520"
     CDS             complement(<1..1045)
                     /locus_tag="VGR29_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2073283.1"
                     /translation="MAVNRGMEQPNVPSSKSGGEAMTASWRLGTIRGIPIGLHWSMAL
                     VFALLTLSLATAFFPATHPDQPVAAYWLMAVISAVLFFTSILLHELGHSWVAQRNGIP
                     VTGITLFVFGGVAQISGRPKTAPVEFRIAAAGPIVSFALAAIFGLVWLLARDFPYLAA
                     PCAWLSRLNLVLALFNLLPGFPLDGGRILRAAVWQITGDERRAAQAAMVSGQLMAFAL
                     MGLGAIMVFRGNFANGVWLIFIGWFIQNAAMTEATGSTLEATLRGVTVAQAMGPREPQ
                     VPSRLKLRQLIDEHVLPTGHRYFLVVDGDIPRGVVTLRDVAKVPRERWDWASVAEVMI
                     PWNRLSYVTPDTEL"
     gene            1523..>3384
                     /locus_tag="VGR29_06525"
     CDS             1523..>3384
                     /locus_tag="VGR29_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073284.1"
                     /translation="MEALFGIPMVRIMVVMVVLLLLCMAVVAFIAWRRPVIFKLGVRN
                     IPRRKAQTILIVIGLMLSTLIISAALGTGDTLNRSVSTQVYDQLGPVDEIIVASADGD
                     GEGEIDAVVTQTIPESSLDTVRDLAGDSGAVDAIGGLLVSYTPALNVEDNDPGDVPSF
                     ERVIETAVQSEPTVILVGIDSTSFEELGGTDDLDGNPVDFASFGEREVLLSETGAEDL
                     SAEVGDYLIVAIRNEPVVLQVAAIAEDSIVTGSLDSSGNPAMAMQLAPLQEMTGNEGR
                     ISGIGISNAGGTREGLENTDAVVNLIKPGLADQGLGINEIKRDLVEDAELIASVFVTF
                     FIVFGLFSIAVGILLIVLIFTMLAAERRSEMGMERAVGAQRRQLIQQFIAEGAGYTLL
                     AGLLGTLLGAAIALGIGKGLQSAFGDFIEITPYVHPRSMVIAYALGVVITFLAVAVSS
                     WRVSRLNVVAAVRDIPDAYQSHRNRRQIIWGIVMLAIGGLLILGAQGSRSLFQFTAGV
                     TLIPFGVAAILTYFGWHPRWVLTVAGLVTLAFWLLPEDIFSSVFGEYDGNIEMFFLSE
                     ICIVAASTRVIMQNFDWLVSGVERLGRRSAGWLPAIRIATSYPTANKGRTGMTLA"
BASE COUNT          653 a         1047 c         1023 g          661 t
ORIGIN      
        1 gaagctccgt gtcaggcgtg acatagctca gccggttcca gggaatcatc acctctgcga
       61 ctgatgccca atcccagcgc tcacgcggca cctttgccac atcgcgcagg gtcactactc
      121 ctcgtggaat atctccatca accacaagga aataccggtg gccggtaggt aggacatgct
      181 catcgatgag ttggcgcaat ttgagccggc tcggaacctg gggctccctt gggcccatcg
      241 cctgtgcgac cgtgacaccg cgcagtgtcg cttcgagcgt ggagcccgtt gcctccgtca
      301 tggccgcatt ttggatgaac caaccaataa agatgagcca tacgccattc gcgaagttgc
      361 ccctgaacac catgatggct cccaagccca tcaatgcgaa agccatgagc tgaccgctca
      421 ccattgcggc ctgtgcggcg cgccgctcat cacctgtaat ctgccagaca gcagcgcgta
      481 atatccgtcc accatcgagc gggaacccag ggaggaggtt gaacagggcc agtaccaggt
      541 tgagccgtga cagccaggcg catggtgctg ccagataggg gaagtcacga gccaacagcc
      601 agaccagacc aaagatggca gcaagcgcga agctgacaat ggggcctgca gcagcgattc
      661 ggaactcgac cggggcagtc ttgggacgac cactaatctg agcgacaccg ccaaacacaa
      721 aaagcgtgat gcctgtcaca gggatgccgt tgcgctgggc tacccaagag tgccccaact
      781 catggagcag gattgaggtg aagaagagca cagcggagat cacggccatg agccagtacg
      841 ctgccactgg ctgatcgggg tgcgtggcag ggaagaaagc cgttgccagc gagagagtta
      901 acagtgcgaa gaccagcgcc atgctccaat gcaggccaat cggaattccc cggatggttc
      961 cgaggcgcca actcgcggtc atcgcctctc cgccgctttt cgaactcggg acgttcggct
     1021 gctccatccc cctattgact gccatctcgc tcatcacctc ctggtgggta acacgccctc
     1081 aggtgatcga cctcacatcc ggcgcatccc gcccttgtcc tcgcgcctcg tggagagcag
     1141 acgccatgcc acccgaaatg tcccggacga gcaccccgga tccgcgtctc ctctcttgac
     1201 tccaagtcgt agaatcgaag tgggtccaca cgagatttgc caatagccac cacgcgtgcc
     1261 cgacagcacg gaatcggttc gtcagttcgt acagggcgga tacactcggc cggcatgctc
     1321 acatgtcagc ggctcgtatg caactcacag gcggcctcat agatgtcggt gcccaggcgc
     1381 accgtatcgt ccggatgcag gacggcgaga tcatcgggga agaccgtgtt ccagccaccg
     1441 tccacgttgc gaatccagac ggtatcgccg caccgcttac cgcctcttcc tgacgaaggg
     1501 gtggctcccg aaggagcaat cgatggaagc cttgttcggt atccccatgg tacgaattat
     1561 ggttgtgatg gtggtgctgc tcctgctctg catggcagtg gtggccttca tcgcgtggcg
     1621 gcgaccggtg atcttcaagc tcggtgtgcg taatatccca cggcgcaagg cgcagacgat
     1681 cttgatcgtg atcggactta tgctctcgac cctgatcatc tccgccgcgc tcggtaccgg
     1741 cgacaccctg aatcggtcgg tcagtaccca ggtctatgat caactcggtc cagtggacga
     1801 aatcatcgtg gcgtcggctg acggagacgg tgagggcgag atcgatgcgg tcgtcaccca
     1861 gaccatcccc gagtcatcgc tcgacaccgt gcgtgacctg gccggcgaca gtggtgcggt
     1921 cgacgccata ggcgggctgc tggtctcgta cacacctgcg ctgaacgttg aggacaatga
     1981 tcccggcgat gttccctcgt tcgagcgggt catcgagaca gcggtacagt ccgagcctac
     2041 ggtgattctt gtcggtatcg attccacctc atttgaggaa ctcggcggga ccgacgacct
     2101 cgacggcaac cctgtcgact tcgccagttt tggcgagcgc gaggtgctcc tgagtgagac
     2161 aggcgccgag gatctcagcg ccgaagtcgg cgattacctc atagtggcga tccggaacga
     2221 gccggtcgtg ctgcaggtgg ctgccatcgc cgaagactca atcgtcaccg ggagcctcga
     2281 cagtagtggt aaccccgcga tggcgatgca actcgcacct ctccaggaga tgacaggcaa
     2341 cgaaggacga atcagcggta tcggcatctc gaacgccggc ggcacgcgag aaggtctgga
     2401 gaacaccgat gctgtcgtca acctgatcaa gccgggcctc gccgaccagg gcttgggcat
     2461 caatgagatc aagcgcgacc tcgttgagga tgcggagctt atcgccagcg tcttcgtgac
     2521 gttcttcatc gtattcggtc tcttctcgat tgcagttggc atcctgctga tcgtcctcat
     2581 ctttaccatg ctcgcggctg agcgccgctc agagatgggc atggagcgag cggtgggggc
     2641 ccagcgccgt cagctgatcc agcagttcat cgccgagggg gctgggtaca cgctgttggc
     2701 cggcttgttg ggtacgctgc ttggtgcggc aatcgcactc ggtattggca agggcctgca
     2761 gtctgccttc ggtgacttca tcgagatcac gccctatgtc cacccgcgaa gcatggttat
     2821 cgcctacgcg cttggtgtgg tgatcacctt cctcgcggtc gcggtgtcat cgtggcgcgt
     2881 gagccggctc aatgtagtag cggccgtccg ggatatcccg gatgcttacc agtcacaccg
     2941 gaatcgccgc cagattatct ggggcatcgt aatgctcgcg atcggcggcc tgctgatcct
     3001 cggtgcacag ggcagccgca gcctgttcca gttcaccgcc ggagtgaccc tcatcccgtt
     3061 cggggtcgcg gcgatcctca cgtacttcgg ctggcacccg cggtgggtat tgaccgtggc
     3121 gggcctcgtc acgctcgcct tctggctact gccggaggac atcttctcgt ccgtcttcgg
     3181 cgaatacgac ggcaatattg agatgttttt cctgtcggag atctgcatcg tggcggcgtc
     3241 gacccgcgtg atcatgcaga actttgactg gctggtctcc ggtgtggaac gcctcggccg
     3301 ccgcagcgcg ggttggttgc cggccatccg gatcgccacc tcgtacccga cggccaacaa
     3361 gggtcgtacc ggcatgacac tcgc
//
LOCUS       DASYWM010000162         6374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_6808, whole genome shotgun sequence.
ACCESSION   DASYWM010000162 DASYWM010000000
VERSION     DASYWM010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6808"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VGR29_06530"
     CDS             <1..763
                     /locus_tag="VGR29_06530"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEV2073285.1"
                     /translation="QRLVPTSRLVSMLTGIEVQVNKAIVGSNAFAHEAGIHQDGMLKE
                     RTTYEIMNPADVGWEGTSLVLGKHSGRAGFRNALKDLGIKLEEDQVQVVYERFLELAD
                     RKKKITGADLEALVADQVSSKAGTLRLVRWSANIGTGGSATASAVIGRGDQEVSGDAE
                     GNGPVDALMKAVDAALGVENELTYFHVEAVTPGEDAQGQVHVRIKHEGQSYSGHGLAT
                     DIVEASAKAYIAALSDVAAHHPDMVLMGSSTSRWS"
     gene            889..1521
                     /locus_tag="VGR29_06535"
     CDS             889..1521
                     /locus_tag="VGR29_06535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2073286.1"
                     /translation="MHRLLDAAGIRVRKLLDLGSGDGIVTTVIAERHPVECAVLVDFS
                     EPMMAAALARFGSLHSGPTVDFVVGDLRSADWHVRVVAAGPYDVVVSRYAIHHLPDED
                     KRALYMEVLDWLVPGGMFVNIEHVSSPSELYEAAHDRLMIDSIVAADGPDADAAAIEN
                     RYWTREDAQANILAPVDVQTRWLIDIGFVDVDCAFKAFELAVFSGRRSAA"
     gene            1594..2853
                     /gene="leuC"
                     /locus_tag="VGR29_06540"
     CDS             1594..2853
                     /gene="leuC"
                     /locus_tag="VGR29_06540"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEV2073287.1"
                     /translation="MSSLRTLAQKLWERHVVRSADGEPDLLYIDLHLVHEVTSPQAFD
                     GLRLAGRPVRRPDLTIATADHNVPTVGIGLPIADPISATQVRKLTENCAEFGVRLHPM
                     GAPGQGIVHVIGPEQGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTL
                     PQFRPETMAITVDGTLAEGVTAKDVILAIIGRIGTGGGAGRIVEYRGEVIRALSMEGR
                     MTICNMSIEGGAKAGMIAPDNTTFAYVEGRKYAPRGAEWEAALDDWRSLPTDEGAEFD
                     HEVVLDGNSIAPHVSWGTNPGQVTSINEVVPDPSQFASEGDRDAAARALKYMALEAGT
                     PIRNIRVDTVFLGSCTNSRIEDLRSAAQILDGRHVADGMRAMVVPGSMRVKAQAEQEG
                     LDKIFRAAGFEWRAAGCSMCLGMNPIS"
     gene            3010..3624
                     /gene="leuD"
                     /locus_tag="VGR29_06545"
     CDS             3010..3624
                     /gene="leuD"
                     /locus_tag="VGR29_06545"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV2073288.1"
                     /translation="MEPVKKIEGRIAPLDRSDVDTDQIIPAVWLKRVERTGFGEGLFS
                     AWRESDPTFVLNRPEFEGTNVLIAGPNFGTGSSREHAVWALMDYGFKAVISPKFGDIF
                     RNNATKSGLVPVQVDEAFGAELLEAAQEHPFLEVVVDIETRTVSVPRLGMQASFPLDD
                     FTRYRLMEGLDDIGLTMTHEPDISSYEATRKAWLPEVDAPAAVG"
     gene            3673..4770
                     /gene="leuB"
                     /locus_tag="VGR29_06550"
     CDS             3673..4770
                     /gene="leuB"
                     /locus_tag="VGR29_06550"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV2073289.1"
                     /translation="MMGTTGSGLERRIVVFGGDGVGPDVMHEALRVLDAVGSSAGHRF
                     TFDQRKLGGCAIDAYGTALRSEDIEAAREADAVLLGAVGGPQWDDPNSRVRPEQGLLG
                     IRKALGLFANLRPVVANEALLDASPLKADRVKGTDLLVVRELTGGLYFGRPSRRWRER
                     RGRAAIDTLIYREHEVRRVVELAFQLAQQRKQHVTSVDKSNVLSSSRLWREIVNEVAE
                     SYPDVWLEHMLVDAMTMKLIQQPSAYDVIVTENMFGDILTDEASVLGGSIGLLPSASL
                     GAAALDGRRPGLYEPIHGSAPDIAGQGIANPAGMILSAALMLRLSFGMEREATGIEQA
                     VEQVVSSGTRTRDLSGTATTSSFGDAVIAAL"
     gene            5153..>6374
                     /locus_tag="VGR29_06555"
     CDS             5153..>6374
                     /locus_tag="VGR29_06555"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEV2073290.1"
                     /translation="MDAKRLVLSLSIAVGLLGSSSASALQEASPGASPMGGPPLPEGC
                     TVIAEGLVNPRYVAVGDDGAIYVSEAGTGGEETIEMPAAGGDATPVDLDATPVAEEGM
                     GHAISTRGGTGQVTMIAPDGTQSVIATGLPSYSIGGPEITGPAGITVDANGQILLAVG
                     GAGPLTLMVEALPNENSVVSIDPATGEVTLLANIDAYERANNPDPNIVDSNLFGIDVA
                     EDGTIYVNDAGGNTTYSVPAGGGEPVLVAVHPGIEIPADQAPPGGNPGRGGANELDPV
                     PTDVLTTADGTVLVSYLSGGPFPPGASKVVSVAADGTLADVVTGLTMTVGLAAGPDGQ
                     LYVSQISTNFLGEMPEPGNVVRVLEDGTQEVVVGGLMLPNGIAFEEDGSLLIVTGATS
                     PGEPMGQLLRCEGIA"
BASE COUNT         1213 a         1759 c         2117 g         1285 t
ORIGIN      
        1 acagcggctg gtgccgacca gccgtcttgt gagcatgctc accggcatcg aagtgcaggt
       61 gaacaaggcc atcgttggtt cgaatgcatt cgctcacgag gccggcatcc atcaggacgg
      121 catgctgaag gagcggacga cgtacgagat catgaatcct gccgatgtcg ggtgggaggg
      181 caccagcctg gtgcttggta aacactcggg gcgggccgga ttcaggaacg ctctgaagga
      241 tctcgggatc aagctcgagg aggatcaggt ccaggttgtc tacgagcgct ttctggagct
      301 ggccgaccgg aagaagaaga ttacgggagc ggaccttgag gcgttggtag ctgaccaggt
      361 ttcctcgaag gccggtacgc tccggctcgt gcgctggagc gcgaacattg gcaccggtgg
      421 atccgcaact gcgtcggccg ttattgggcg cggtgaccag gaggtctccg gggatgctga
      481 gggcaatggt cccgtcgatg cattgatgaa ggccgtagac gcggcgctcg gcgtcgagaa
      541 cgagctgacg tatttccatg tcgaagccgt gactccgggc gaggatgccc agggacaggt
      601 gcatgtgcgc atcaagcacg agggtcagtc ctactcgggg cacggcctgg caaccgatat
      661 cgtggaagcg agcgcgaagg cgtacatcgc ggcactgagt gacgttgcgg cgcaccatcc
      721 ggacatggtg ttgatgggct cgtcgacgtc acgctggagc taagcgatgg cgatgcgcgc
      781 ggggagccca cggaacgtca cgaccgggtc tggcaactac cggctgtagc gtccgggtat
      841 catgacatcc gggatggcgt tccgttcgta gaggcgcagt tcgaggtcat gcaccggcta
      901 ctcgatgcgg ccggaatccg tgtgcggaag cttcttgacc tcggctctgg cgatggaatt
      961 gtcactaccg ttattgcaga gcggcatccc gtagaatgcg ccgtactggt cgatttttct
     1021 gagccaatga tggccgcggc acttgcgcgg ttcggttccc tacattcagg gccaacggtg
     1081 gatttcgtcg taggcgatct tcgctccgca gactggcacg tccgggtagt tgccgcaggg
     1141 ccatacgatg tcgtcgtctc ccgctatgcc attcatcacc tgccggacga agacaagcgt
     1201 gccctgtata tggaagtgct tgactggcta gttccgggag gtatgttcgt caacattgag
     1261 catgtgagct ccccgtccga actctacgag gcagcgcatg atcgcctgat gatcgactcg
     1321 attgtggcgg ccgatgggcc ggacgcggat gcggcggcca tcgagaatcg ctactggaca
     1381 cgtgaggatg cccaagcgaa catcctggct ccggtcgacg tccagacacg ttggctcatc
     1441 gatatcgggt tcgtcgatgt cgattgcgcg ttcaaggcgt ttgagcttgc ggtgttctca
     1501 ggccggcggt cagctgcata acgacaatat cttataaatt tgatcgagac agtcttacga
     1561 actttgagag gtagtcacgt ggaggcaaat acggtgtcgt cgcttaggac gttggcccaa
     1621 aaactgtggg agcgccatgt ggtgcgctcg gcggacggcg agcccgatct gctctatatc
     1681 gatttgcatc ttgtgcatga agtgacgtcg ccgcaggcgt ttgacggtct caggctggct
     1741 ggtcgcccgg tgcgacgtcc cgacctgacg atcgcaacag ctgaccacaa cgtcccaact
     1801 gttggcatcg gactccctat tgccgacccc atctcggcca cacaggtccg gaagctgacc
     1861 gagaactgtg cggaatttgg cgtccggctg catcccatgg gtgcgcccgg tcagggcatc
     1921 gtgcacgtca ttgggccgga gcaggggttg acccagccgg gtatgacgat cgtctgtggg
     1981 gacagccata cgtccaccca tggcgctttt ggcgcgctcg cttttggcat cggcacgagc
     2041 gaagtcgaac atgtccttgc cacgcagacg ctgcctcagt tccggcccga gacaatggcg
     2101 atcacggtgg acggcacgct ggcggagggc gtgacggcga aggatgtcat cctggcgatc
     2161 attggcagga tcggcaccgg cggcggtgcc ggtcgcattg ttgagtaccg cggcgaggtc
     2221 atccgtgctc tttcgatgga agggcggatg acgatctgca acatgtcgat tgagggcggc
     2281 gccaaggcgg gcatgattgc gcctgacaat acaactttcg cctatgttga gggtcgcaag
     2341 tacgcgccaa gaggtgctga atgggaagcg gcacttgacg actggaggtc gcttcccacc
     2401 gatgaagggg cggagttcga ccatgaggtg gtgctggacg gcaacagcat tgctccgcat
     2461 gtttcgtggg gcacgaatcc tggccaggtg acgtccatca atgaggttgt gcccgatccg
     2521 tcgcagtttg caagcgaggg cgaccgcgat gccgcagcgc gcgccctgaa atacatggcg
     2581 ctggaggccg gcacaccgat ccgtaatatt cgggtcgata ctgtcttcct tgggtcgtgc
     2641 acgaatagcc ggatcgagga cctgcgctcg gcggcgcaga tcctggacgg tcgccatgtg
     2701 gctgatggca tgcgggccat ggtggttccc ggctccatgc gggtgaaggc gcaagcggaa
     2761 caggaagggc tggacaagat cttccgggcc gccggcttcg agtggcgggc agcggggtgt
     2821 tcaatgtgtc tcggcatgaa tccgatcagc tgagtcccgg cgagcgctgc gcatccacca
     2881 gcaaccgcaa tttcgaaggg cggcagggca agggcgggcg cactcatctc gtatcgccgc
     2941 aagtcgctgc cgctaccgct gtgatgggga cgttcgcaac gccgggcgac ctggcttagg
     3001 aggaacgcgg tggaaccggt caagaaaatc gaggggcgga ttgcaccact tgatcgcagc
     3061 gatgtcgata ccgaccagat tattccggcc gtctggctca agcgggttga gcgaaccggc
     3121 tttggcgaag gattgttctc tgcctggaga gaatccgacc cgacctttgt cctcaatcga
     3181 cccgagttcg agggcacgaa cgtccttatt gccggtccaa acttcggcac gggctcctcg
     3241 cgcgagcatg ctgtctgggc gctgatggac tacgggttca aggctgtcat ctctcccaag
     3301 ttcggcgata tcttccggaa caacgcgacc aagtcgggcc tcgtgccggt ccaggtggat
     3361 gaagcgttcg gcgccgagtt gcttgaggcg gcacaggagc atccgttcct ggaggtcgtc
     3421 gtggatattg agaccaggac ggtttccgtg ccccgtttag ggatgcaggc aagcttcccg
     3481 ctggacgact tcacccggta ccgattgatg gaaggcctgg acgatatcgg gctcacaatg
     3541 acgcacgagc ctgacatctc gtcatacgaa gccacgcgga aggcatggtt gcccgaggta
     3601 gacgcgcctg cggcagttgg ataggacgag aagggcgatc atgatcgatc gcccttcggc
     3661 gcggttgggg agatgatggg gacgacaggc agcggtttgg agaggcggat cgtcgtgttc
     3721 ggaggcgatg gcgttgggcc agatgtgatg catgaggcgc ttcgcgtgct ggatgcggtc
     3781 ggctcatcgg ctggacatag attcaccttt gatcagcgca agttgggcgg ttgtgcgatc
     3841 gatgcatatg ggaccgcact gaggtccgag gatatcgagg ccgctcgtga ggctgatgcg
     3901 gtgttgctcg gcgcggtggg cggaccccaa tgggatgacc caaactccag ggtcaggccg
     3961 gagcagggcc tccttggtat ccgcaaggcg cttggcctgt ttgcgaatct ccgcccagtt
     4021 gtcgccaacg aggctcttct cgacgcgtct ccgcttaagg cggatcgtgt caaggggacc
     4081 gacctgctgg tcgtccgcga actgacaggc gggctctatt tcgggcgtcc ttccaggcga
     4141 tggcgcgagc gccgcggacg tgccgctatt gatacactaa tctatcgtga acatgaagtc
     4201 cgccgtgttg tagaactcgc attccagctt gcgcagcagc ggaagcagca cgtcacgagt
     4261 gtcgacaaat ccaacgtgct ctcatcttcc cgattgtggc gggagatcgt gaatgaggtc
     4321 gcggagagct atccggatgt ctggttagag cacatgctgg ttgacgcgat gaccatgaag
     4381 ctgatccagc agccgagtgc ctacgatgtg attgtcacgg agaacatgtt cggggacatc
     4441 ctcaccgatg aggcctcggt gctgggaggg tcgattggcc tgcttccgtc agccagcctt
     4501 ggcgccgcag cattggatgg caggcggcct gggctatacg agccgattca cgggagcgcg
     4561 cccgacattg cgggacaggg aatcgcaaac ccggcgggca tgattctctc cgcggccctg
     4621 atgcttcgcc tctcgtttgg catggaacgg gaagcgactg gaatagagca agccgttgag
     4681 caggtggtaa gctcaggtac ccggacccgc gatctcagtg gaaccgctac gacgtcatct
     4741 ttcggcgacg cagtcattgc tgcactgtag tggaatgatg ggagggctgc acaaaccatg
     4801 gcagccctct ggttccgctt atctcttata catttcgctt cggggctgtg agagtcccaa
     4861 atgaaggact ccgaagacgg gcgatgcagc agccggcggg ccaacctggc ggcgtcgtcc
     4921 tgtgtccggg tttagatatc cattgcccca tcagatgtct tgtagcgcat cgtcttcctt
     4981 acgcccgcgg cgtgtccttc ttgctcccaa gagctgagct tcccagatgc gccggaaggt
     5041 caatcttagg atttaagcct atttcccgga tctgccattg aatgttgaat gactgatgcg
     5101 tagaattgcg aatgggtccc aattggaata ggtcggtcca gaaggagagt gtatggacgc
     5161 gaagcgttta gtgctcagtc tcagtatcgc tgtaggtctc cttggaagta gttccgcctc
     5221 ggcgttgcag gaagctagtc caggtgccag tcccatgggc ggtcctccac tgccagaggg
     5281 gtgtactgtc atcgccgaag ggctcgtcaa tccgcgatac gttgctgtcg gcgacgatgg
     5341 agctatttac gtttctgaag cgggtaccgg tggtgaggag accatcgaga tgccagccgc
     5401 tggtggcgat gcgactccgg tcgatctcga tgcaacaccg gtcgccgaag aggggatggg
     5461 tcatgcgatc agcacccgag gtggtaccgg tcaagtcacc atgatcgcgc ccgacggcac
     5521 acaatccgtt attgcgaccg ggctgccgtc gtatagcatc ggaggaccgg aaatcaccgg
     5581 accagcaggt atcacggtgg acgccaatgg tcagattttg ctggcggtag gtggcgcagg
     5641 gccccttaca ctgatggttg aagcactgcc gaatgagaac tcggtggtga gcatagatcc
     5701 ggccactggc gaagtgaccc tactcgccaa tatcgacgcc tatgagcgcg ccaataaccc
     5761 ggatcccaac attgtagatt cgaatctctt cggtatcgat gtcgctgaag acggcacgat
     5821 ctacgtgaac gatgctggtg gaaacaccac ctacagcgta ccggcaggcg gcggtgaacc
     5881 cgtgctcgtt gcggtgcatc caggcatcga gatacctgcc gaccaggctc ctccgggcgg
     5941 taacccgggc cgaggtggcg ccaacgaact ggacccggta cccaccgatg tcctgaccac
     6001 tgctgacggc accgtgcttg tcagctatct gagcggtggg ccgttcccgc cgggagcttc
     6061 gaaggttgtg agcgtggccg ccgatggtac cctggccgat gtcgtgacgg gtctcaccat
     6121 gaccgtgggt ctcgccgcag ggccagacgg gcagctctac gtttcccaga tctcgacgaa
     6181 tttcctcggc gaaatgccgg aaccgggcaa cgtcgtccgc gtgctggaag atggcacgca
     6241 ggaagtggtc gttggcggcc tgatgctgcc gaacggtatc gcgttcgagg aagacggcag
     6301 cctgctgatt gtcacaggtg cgaccagccc tggcgagccg atgggccagt tgctgcgctg
     6361 cgagggcatc gctg
//
LOCUS       DASYWM010000163        10702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_213110, whole genome shotgun sequence.
ACCESSION   DASYWM010000163 DASYWM010000000
VERSION     DASYWM010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10702
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213110"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VGR29_06560"
     CDS             <1..655
                     /locus_tag="VGR29_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEV2073291.1"
                     /translation="GTTWPVSLPLLGDFNVSNALCAVGVASAAGLEIPVIVDALADAS
                     GVPGRLNPLNEGQPFSVVVDYAHTPASLSKVLRLLRELHACGRLIVVSGSAGERDPGK
                     RPLQGAVMAELGDVSIVTSEDPRNENPEAIIRQIADGARGQGAIDGETVFEIVDRREA
                     ICHAMTIARAGDCVLLPGKGHETSIIWGFEHRPWNEAGVAREELIRLGFRSPEELRA"
     gene            652..1626
                     /locus_tag="VGR29_06565"
     CDS             652..1626
                     /locus_tag="VGR29_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073292.1"
                     /translation="MITRRRFGSVATGVLGAALLSPRPRHAAGQGGEPEGKIAFIKDG
                     DIWEWSSQDGAQPIVEDGNAMDPTWNPRGNILLYARDGGSFSDLIQANPRTGNLRRLT
                     DNESPEEPGSPEYVSGCSWAIDPCWSAADIVCYVSDAASEFGEMELWILDPDNGEAYV
                     APDDGNEQGPVESVSVDASAVYAVYTVLAAGGEEGGTTYVSMRDLNVGTTYPLIEGPM
                     GAYDPAISPDGAWVVASIRDEAGTSDLWLLNRVEETLVRLTEGEEASNATWSPDGDWI
                     AYLRWEGSGFELKAFPIDPAAGERIGEAKRLADADSISATSRLSWANL"
     gene            complement(1761..3611)
                     /gene="aspS"
                     /locus_tag="VGR29_06570"
     CDS             complement(1761..3611)
                     /gene="aspS"
                     /locus_tag="VGR29_06570"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV2073293.1"
                     /translation="MQQIDVQPDVVGRTTCGSLRPGDADSDVVLKGWVNRRRDLGGLI
                     FIDLRDRFGITQVVFNPQVASRAHEQANRLRNEFVIEVKGVVRLRPEGTVNHKMATGE
                     IEVEAHELTILNESKTPPFYINEEVDVDESLRLKFRYLDLRRQKMQHNVILRHRIVKF
                     MRDYLDQRGFVEVETPLLIKSTPEGARDFLVPSSSMPGSFYALPQSPQILKQLLMVSG
                     LDRYFQIARCFRDEAQRADRQPEFTQLDIEMTFITQEDIMSMMEKLYTEITQHFTAKR
                     IQQAPFPRLTYAESMDRFGNDRPDLRFDLELQNVSGSLRDTGFKAFAGVLQSGGEVKA
                     IVVPGCGGYTRKQIDEVTDLARQAGAKGLATIAVTEEGVRSPIAKFLSQDELAALTTG
                     IGAGTGDLVVLVADQPAIVAKTLSSLRDEFGRRLGLADPDVLAYCWVYEFPLLEWDEE
                     GNRWDATHNPFSGYLEEDEALLDASPGDVRAKQYDLVGNGNELGGGSVRIHKRASQEK
                     VFGLMGHSGEGLQERFGALLDALEYGAPPHGGIAMGIDRFAMLLADEESIRDVIAFPK
                     NQRGIDMMFESPAPVEDMQLEELGLAIRQGVALEAPNIEIDLPDEPGTNI"
     gene            complement(3712..5052)
                     /locus_tag="VGR29_06575"
     CDS             complement(3712..5052)
                     /locus_tag="VGR29_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HEV2073294.1"
                     /translation="MTVVTPDITIVGLGPGDPGQRTIAVQRALDSAARIFVRTHPDVD
                     LSDLVEHEHVTDVASLRQPDTELGGRWQVANDAVCDAAADGPVVLAIPGHPRFGEGLV
                     VGTLNEAARRGLSTRVLDGISVVDLMATTLGIDPLLQDAQFFNAREFGTEDDPQSPFM
                     GGMFTGSPLRPMLLTHVYDQAVMAGVARALARVLPHDHSVIRIVAAGMPDEEISHHTV
                     GELPIVPSGPLVALWVPAQGDLEAGRDPRTLQHIVARLRRPDGCPWDREQTHESLRDN
                     IVDEAYEVMDAIDAGDPVNLVEELGDLFLLICMHAQIAEENGTFTLEDVYQGISSKII
                     RRHPHVFGDAVANEASDLAEIWAGVKAQEKADNPLRAVKGIDGQPHSMPALVRAPKVL
                     RKYPLTTDMPPSTQDDRSRELLRAVAAIIMAGDDPEHVLRDALAEHVTGNATPP"
     gene            complement(5049..6890)
                     /gene="lepA"
                     /locus_tag="VGR29_06580"
     CDS             complement(5049..6890)
                     /gene="lepA"
                     /locus_tag="VGR29_06580"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEV2073295.1"
                     /translation="MSHTIDTSHIRNFSIIAHIDHGKSTLADRLLQITGTVSERDLGT
                     QMLDTMDLEREKGITIKARAVRMKYTAQDGQTYELNLIDTPGHVDFSYEVSRSLAACE
                     GALLVVDAAQGIEAQTMANVYLALEHDLAMIAVINKIDLPNAEPERVSKEVGDFIGLV
                     DDEIIPASAKTGIGVENILEAIVNHIPPPSPEDGTRPLRALIFDSHYDAYKGVIAYVK
                     IVDGELHADERIRILGTKKEADILEVGYFAPLMQSSESLRTGEVGYIATGLKVVKDCQ
                     VGDTITLAREPAEKPLPGYQPAKAMVFAGLYPVDSEDFPILRDALERLQLNDASLTYE
                     AESSMALGFGFRCGFLGLLHMEIIQERLEREYDLDLLATAPSVEYEVVLNSGKIVRVD
                     NPSDMPNFGEIQAIREPWMHLNIIAPSRFIGAIMELTTSKRAMFIEMIYLDEDRVQLK
                     YDIPLAELIVEFYDQLKSRTQGYASLDYSLADMREADLVKLDVLVNGQPVDALSVITH
                     RDDAYYKGRELVQQLRKLIPRQMFDVPIQASIGSRIISRETVKAMRKNVLAKCYGGDV
                     SRKRKLLEKQKEGKARMKMVGSVEIPQEAFMAVLSLGSSSDKTAAKG"
     gene            complement(7120..8805)
                     /gene="murJ"
                     /locus_tag="VGR29_06585"
     CDS             complement(7120..8805)
                     /gene="murJ"
                     /locus_tag="VGR29_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEV2073296.1"
                     /translation="MSQASQVFGNPVSAETSTNATEDTTRSAGATMARNAIVVSIAFI
                     LSRLLGLGREMILANQFGTGPEMDAYVSAFRIPDLLFLTVMSGAFGAAFIPVFAGFID
                     QGDREQASRLASSIMTWTGLGIVILAGISFLLARPLTNLVAPGFDPATEDLAVNLMRI
                     LLMSPVFLGFGIAAKGILEAQNQFTLPALAPIVYNLAIIIGALFFAADHGIYAVGWAV
                     IAGAIAHVGIQIPGLMRSGFHFRPTLDRSITGLAEVLRLLGPRVVGLAAFQVNFIAVN
                     AFASTIDARNVSALNYAWQLLMLPHGVLALSISTVAFPSLAALYSRGDRAGFGRLLDR
                     TMRPLIFLSVPASIGLWLVGRPIVIVIFESGDFDEASTDLVSSALNWFAIGLVGYGIT
                     EILTRVFYAMRDTRTPVITGILTIVLNVVLCALFIQSLGHRGLALSLSVTTAAEAVIM
                     MLFLKNRSGRVFSPGFFEWLVKVLLASAAMVVAIAITVPWLNEVLRADVFIAVRLLYF
                     ATCMALYTLTFLFAAWTLRIPEFHSSVSKVLDRLPGRVRARMIWLGIDEPSGA"
     gene            9005..9277
                     /gene="rpsT"
                     /locus_tag="VGR29_06590"
     CDS             9005..9277
                     /gene="rpsT"
                     /locus_tag="VGR29_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV2073297.1"
                     /translation="MANSKSAEKRIRTSERRRLHNRPYRSAARTLVKKAELAIQAGDQ
                     AAAQTAVIQAVSMLDRIATKGIVHRNNAARRKARLMAKFNAVAASA"
     gene            complement(9399..10430)
                     /locus_tag="VGR29_06595"
     CDS             complement(9399..10430)
                     /locus_tag="VGR29_06595"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073298.1"
                     /translation="MICIILGPDYAMARARVNTIRKQRDSSGDSTSMLDGKAVSIRQI
                     IMDVSSIGFFSAGRVVIVEDLIARLGKQGARDGGNTPDWSALFSAVPDASTLILLDPS
                     LQSLPAAVKKALPPTASIDLSDPPRGPALLKWIQGTAKTIGSEIDQETARLLAELLYP
                     QTWMQKSRNPAFDRPPDMEMLSNEIAKLALAAHPGTITRNLVSELVDRGSDDRIFTFL
                     DAAVAGNVGIAMVELDKLLEAGEDPAKLLAQLSQNVELGAVMATAGRRDPAEVGKAVG
                     VTSAGRMSSIRQSLQGQSSGTALGRVDIATAADRKMKRGELRDPLDALYDAVLGIAAS
                     RQMPHRAGK"
     gene            complement(10518..>10702)
                     /locus_tag="VGR29_06600"
     CDS             complement(10518..>10702)
                     /locus_tag="VGR29_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073299.1"
                     /translation="SYPLRHGRSALMGPLEDATLDVRPVLPDAVALDVVLSQRFVSRE
                     LLERGSWFTQAAAPTC"
BASE COUNT         2183 a         3087 c         3299 g         2133 t
ORIGIN      
        1 ggggacgact tggcctgttt cccttccact attgggtgat ttcaatgttt cgaatgcgct
       61 ttgcgctgtc ggcgtagcaa gcgccgctgg tttggagatc cctgtcatcg tcgatgcatt
      121 ggcagacgca tccggggtcc ccggtcgcct gaatcccttg aatgaaggac agccgttctc
      181 tgttgttgtg gactacgctc acacgcccgc atcgctcagc aaggtgctta gactcctccg
      241 agagctgcat gcctgcggac ggctcatcgt cgtctccggg agtgcaggtg agcgggatcc
      301 aggcaagaga ccgctccagg gagccgtcat ggctgagctc ggcgatgtca gcattgtgac
      361 gagcgaggat ccccgaaacg agaatcccga ggccatcatc cgtcagatag cggacggagc
      421 gcgaggtcag ggcgctattg atggagaaac cgtgttcgaa atcgttgacc gccgcgaggc
      481 gatctgtcat gcgatgacga tcgcacgggc gggcgactgc gttctgttgc caggtaaggg
      541 gcatgagacg agcattatat ggggtttcga gcatcgaccg tggaatgagg ccggcgtagc
      601 acgcgaggaa cttatccgcc tcgggttccg ctcccccgaa gagctgcgcg catgattact
      661 cggcgacgat tcggatcggt cgcaacgggg gttcttggtg cggcgctcct ttcaccccgg
      721 ccgcgccatg ctgctggtca aggcggcgag cccgagggaa agatcgcctt catcaaggat
      781 ggcgacatct gggagtggtc ctcgcaggac ggtgcgcaac cgatcgtgga ggacgggaac
      841 gcgatggacc caacatggaa tccgcgcggg aatatcctgc tctacgcccg tgatggcgga
      901 agcttctctg acctcatcca ggccaatccc cgtacgggga acctgcggcg gctgacagac
      961 aacgaaagcc ctgaggagcc ggggagccca gaatacgtga gcggttgctc ctgggcaatc
     1021 gacccgtgct ggagtgcggc ggatatcgtc tgttacgtca gcgatgcagc gtcggaattc
     1081 ggagagatgg agctctggat cctggatccg gataacggcg aagcatacgt ggcaccagat
     1141 gacggcaatg aacagggacc ggtagagtcg gtttctgtcg atgcatcagc ggtctatgcc
     1201 gtctacacgg tcttggcggc cggcggggag gagggcggca caacctatgt gtcgatgcgt
     1261 gatctcaatg taggaacgac atatcctctg attgagggac caatgggcgc atacgatccc
     1321 gcaatttctc cagacggggc ctgggttgtc gcctccatca gggacgaagc tggcacgagt
     1381 gatctctggc tcttgaatcg cgtcgaggag acgctggtgc gccttactga aggtgaggaa
     1441 gcatccaacg ccacctggtc tccggatgga gattggatcg cgtatctcag gtgggagggg
     1501 tcagggttcg agttgaaggc gtttcccatc gatccagcag ccggcgagcg gataggggag
     1561 gcgaagcgac tggccgacgc tgactccatc agtgctacca gccgactctc atgggcgaat
     1621 ctctaaatac catcccataa agccttgagg ttggacttcc aattgatgct ctgacgcacc
     1681 gtgcctctgt cattccggga cggcggaagc ccggaaggag atgcctcccg ccgggcttct
     1741 tcattgcccg tccatcaaag ttagatgttc gtccccggct cgtctggcaa atcgatctct
     1801 atgttcggag cctccagtgc caccccctga cgaatggcga gacccaactc ctcgagctgc
     1861 atatcttcga ccggagcagg tgattcaaac atcatgtcga taccgcgttg attcttggga
     1921 aacgcgataa cgtcgcgaat actctcctca tcggcgagaa gcatggcgaa tcggtcgatg
     1981 cccatggcga tcccgccgtg cggaggtgcg ccatattcga gggcatccag cagggccccg
     2041 aaacgctcct gcagtccctc gccggagtga cccatgaggc cgaaaacctt ttcctggctc
     2101 gcccgtttgt gaatgcgcac gctgccacca ccgagctcat tgccgttgcc gacgaggtcg
     2161 tattgcttgg cgcgaacgtc gccgggcgag gcgtcaagaa gcgcttcgtc ctcttcgaga
     2221 tagccggaga aggggttgtg tgtcgcgtcc cagcgattgc cctcttcatc ccactcaagc
     2281 aacgggaact catagaccca gcaatatgcc aggacatccg ggtccgccag tccaagtctc
     2341 ctgccgaact catcgcgtag tgaggacaga gtcttcgcca caatggctgg ttgatcggcg
     2401 acgagcacga ccagatcgcc ggtgcctgca ccaatgcccg tggtgagggc cgcgagctcg
     2461 tcctgggaaa gaaacttcgc aataggcgat ctcacgcctt cctcggtcac cgcgatcgtt
     2521 gccagcccct ttgcgcctgc ctgcctggcg agatcagtaa cctcatcgat ttgcttgcgg
     2581 gtatagccgc cgcatcctgg cacgacaatg gccttgacct cgccgccgga ctgaagtaca
     2641 cctgcgaacg ccttgaaacc ggtatcgcgt agcgacccgg acacgttctg cagctccaga
     2701 tcgaagcgga ggtccggcct gtcattgccg aaccggtcca tcgactctgc ataggtcagg
     2761 cgtgggaacg gagcctgctg gatcctcttt gcagtgaagt gctgagtgat ctcggtgtag
     2821 agcttttcca tcatcgacat gatgtcttcc tgagtgatga aggtcatttc gatgtcgagc
     2881 tgggtaaact ccggctgacg atctgcacgc tgggcttcat cccggaagca ccgggcgatc
     2941 tggaaatagc ggtccaggcc ggacaccatc agaagctgct tcaggatctg gggcgactgt
     3001 ggcagggcat agaaggaccc cggcatgctg ctacttggaa caaggaaatc gcgggcgccc
     3061 tccggcgtgc tcttgatcag cagtggagtt tccacctcta cgaagccgcg ctgatcaagg
     3121 tagtcgcgca tgaacttcac gatgcgatgg cgaaggatga cgttgtgctg cattttctgg
     3181 cgacggaggt cgaggtagcg gaacttgagg cgcagcgatt catccacatc cacctcttca
     3241 ttgatgtaga aagggggagt tttggattcg ttgaggattg tcagttcgtg tgcttcaacc
     3301 tcgatttccc cggtcgccat cttgtgattg acggtgcctt ccggacgaag ccggacgacg
     3361 cccttgacct cgatcacgaa ctcgttgcgg aggcgattgg cctgttcgtg ggcccgggat
     3421 gctacctgcg ggttgaagac aacctgcgtt atgccgaaac ggtcgcgcag atcaatgaag
     3481 atcagccctc caaggtcgcg acgccggttc acccacccct taagcaccac atctgaatcg
     3541 gcatctcctg gcctgagcga tccgcaggta gtgcgaccta ccacatcggg ctggacgtca
     3601 atctgttgca tgaaacatat cctcctgttt tcacggtccg atagcgcagg ggagcacgtg
     3661 gtatgctcgc ggcgtcaacg aaaaaattac gagagcacac aaggtgcttc cctacggtgg
     3721 tgtagcgttg ccggtcacat gctcggctag cgcgtctcgg aggacatgtt cggggtcatc
     3781 acccgccatg atgatcgcgg cgaccgccct caacagctca cgggaacggt cgtcctgggt
     3841 tgatggaggc atgtcggttg tgagcggata tttcctcagg acctttggtg cccggacaag
     3901 cgctggcatg gaatgcggct gcccgtcgat ccccttcacg gcgcgaaggg gattatccgc
     3961 cttttcctgg gccttgactc cggcccaaat ctcggccagg tccgatgcct cgttcgccac
     4021 tgcatcgcca aaaacgtgtg gatggcgccg gataatcttg gacgaaattc cctggtagac
     4081 gtcttctagc gtgaaggtgc cattctcttc ggctatctgg gcatgcatgc agataagaag
     4141 gaagagatcg cccagttcct cgacgaggtt gaccgggtcg ccggcatcga ttgcatccat
     4201 gacctcatac gcttcatcga cgatattgtc gcggagcgac tcatgcgtct gttcgcgatc
     4261 ccagggacag ccgtcgggac gccggagacg tgccacgatg tgctgcagcg ttcgggggtc
     4321 gcgcccggct tccaagtccc cctgggcagg cacccagagc gcgacaagcg gcccactggg
     4381 aacaattgga agctcgccaa cagtgtgatg actgatctcc tcgtccggca taccagcagc
     4441 aacgatgcgg atgacgctgt ggtcgtgcgg caggacgcgc gcgagagcac gcgctacacc
     4501 ggccataact gcttggtcgt agacatgggt caggagcatt ggcctaagag gggagccggt
     4561 gaacatgccg cccatgaagg ggctctgtgg atcatcctcg gttccgaact cccgcgcatt
     4621 gaagaactgc gcgtcttgca gcagtgggtc gatacccagc gtggttgcca tgaggtcgac
     4681 aacactgatg ccatcgagga cacgggttga taggccgcgt ctggccgctt cgttcagcgt
     4741 ccccaccacc agtccctcgc caaatcgcgg atgaccggga atggcgagaa cgaccggacc
     4801 atcggccgcg gcgtcacaga cagcgtcatt tgcaacctgc cagcggccac ctagctcggt
     4861 atccggctgt cgaagactgg ccacatccgt aacatgctcg tgttccacga ggtcgcttag
     4921 atcaacgtcg ggatgcgttc ggacgaaaat ccgggctgct gagtcgagtg ccctctgtac
     4981 cgcaatggtt cgttggccgg ggtcacccgg accgagaccg acgattgtga tgtcgggagt
     5041 gacaacggtc atccctttgc tgccgtcttg tcggaggaac tgccgaggct caggacagcc
     5101 atgaacgctt cctgcggaat ttcgacgctg cctaccatct tcattcgcgc cttgccctct
     5161 ttttgcttct ccagcagctt gcgctttcgg ctgacgtcgc cgccgtagca tttggcgagc
     5221 acattcttgc gcatcgcctt tacggtctcg cgagagataa tccggctgcc gatcgaggcc
     5281 tggattggta cgtcaaacat ctgccggggg atgagcttgc gcagctgctg cacgagttcc
     5341 cgccccttgt aataggcatc gtcccggtgc gtgatgaccg agagggcatc gacaggctgg
     5401 ccattcacca gcacgtcaag ctttaccagg tccgcctccc gcatatcggc caacgagtaa
     5461 tcgagcgagg cgtatccctg cgtcctgctc ttaagctggt cgtaaaattc gacgatcagt
     5521 tcggcaagag ggatgtcgta cttgagctga acgcgatcct cgtcgaggta aatcatctca
     5581 atgaacatgg cgcgcttgct tgtcgtgagc tccatgatgg ccccaatgaa gcgcgagggc
     5641 gcgatgatgt tgaggtgcat ccagggttcg cggatcgcct ggatctcgcc gaagttcggc
     5701 atgtcagagg ggttgtctac gcgtacgatc ttgccgctgt tcagcacaac ctcatactcc
     5761 acgctaggcg ccgtcgccag caggtcgagg tcgtattcgc gctcgaggcg ctcctggatg
     5821 atctccatat ggagcagtcc gagaaagccg caccggaagc cgaagccaag tgccatcgag
     5881 gattcggcct cgtaggtgag tgacgcatca ttgagctgga gtcgctccag ggcatcgcgg
     5941 agaatcggaa agtcctcgct gtctaccggg tagaggccag cgaagaccat cgccttcgct
     6001 ggctggtagc cgggaagagg cttctccgcc ggttcacggg caagagtgat cgtgtctccg
     6061 acttggcaat ccttgacgac tttgagcccg gttgcgatgt agccgacctc acccgtccgc
     6121 aagctctcag aggactgcat cagcggcgcg aagtacccga cttcgagaat atcggcttct
     6181 ttcttcgtac ccaggatacg gatgcgctcg tcagcgtgga gctcgccatc gacgatcttg
     6241 acgtaagcta ttacaccctt gtacgcgtca tagtgggagt caaagataag cgcccgcaac
     6301 ggtctggttc cgtcctcagg agatgggggc gggatgtgat taacgatcgc ttcgaggatg
     6361 ttctcgacgc cgattcccgt cttggccgag gcggggatga tctcgtcatc taccagacca
     6421 atgaaatcgc ccacttcctt gctgacccgt tcaggctcgg cgtttggcag gtcgatttta
     6481 ttgatgaccg cgatcatcgc gaggtcgtgt tcgagagcga ggtagacgtt ggccatcgtc
     6541 tgcgcctcga ttccctgggc ggcatcgacg acaagcagcg caccctcaca ggcggcgagg
     6601 ctgcggctga cctcgtagga aaagtcaaca tgaccggggg tgtcgatcaa gttgagctcg
     6661 tacgtctgac cgtcctgagc cgtgtacttc atccgtaccg cgcgggcctt gatggtgatg
     6721 cccttttcac gctccagatc catggtgtcc agcatctggg tcccgagatc tcgctcggat
     6781 accgtcccgg tgatctgcag aagacgatcg gccagcgtgg atttgccgtg gtcgatatgg
     6841 gcgatgatgc tgaagtttcg gatgtgggac gtgtcgatcg tatggctcat gtggagcgag
     6901 ggttcccgtc taggtgtctg gcatgcgctg caaattggcc gggagcttca gcgcctggct
     6961 gccaccccgg ccatattcgc cccaagtata gccattgtcc cggagcgctg acgttgaaga
     7021 tgccgctggt caccccgcgg aagacatttc agtgtactca ccgtcgttcc cgataaccga
     7081 tgcccgcacc actcttgcgg cgatcgtcgc tgcgccgaat caggcgccgg agggctcgtc
     7141 gataccaagc cagatcatcc gtgcacggac gcggccaggc aaacgatcca gcactttgct
     7201 taccgagctg tggaactcgg ggatacggag ggtccaggcg gcgaagagga atgtcagcgt
     7261 gtagagtgcc atgcaggtcg cgaagtagag aaggcgaacg gcaatgaaaa catccgccct
     7321 gagaacctca ttgagccacg ggaccgtaat tgcgatcgcg acaaccatcg cggcgctcgc
     7381 cagcaagacc ttcaccaacc actcgaagaa gccgggagag aagactcgtc cggagcggtt
     7441 cttcaggaaa agcatcatga tgaccgcctc cgctgctgtg gtgacgctga gcgagagggc
     7501 gagacctcgg tgccccagag actggataaa gagagcgcaa agaaccacgt tgagaacgat
     7561 ggtcaggatt cccgtgatca cgggcgtcct cgtgtcacgc atcgcataga agacgcgtgt
     7621 gagaatctcc gtgatgccat atccgacaag cccaatagcg aaccagttga gcgccgagct
     7681 cacgaggtca gtcgatgcct cgtcaaagtc accgctctcg aagatgacaa tcacaatggg
     7741 gcgtccgacg agccacaggc caatgcttgc gggcaccgaa aggaagatga gcggtcgcat
     7801 ggtgcggtct agcaggcggc cgaatcccgc acggtcgccc cggctgtaga gcgccgccag
     7861 agacgggaac gcgaccgtgg aaatactgag cgcaagcact ccatgcggaa gcatgaggag
     7921 ctgccacgcg tagttgaggg cggagacgtt gcgggcatcg atggtggatg caaatgcgtt
     7981 cacggcgatg aagttcacct ggaacgctgc gagtcctaca acacgcggtc caagcagccg
     8041 gagaacctca gccaggccag tgatcgagcg atcaagcgtg gggcgaaagt gaaaccctga
     8101 ccgcatgagg ccaggaatct ggataccgac gtgagcgatg gcgccggcaa tgacggccca
     8161 gccaaccgcg tagattccgt gatcggctgc aaagaagagg gcaccgatga tgatcgcgag
     8221 gttgtagacg atcggggcga gggcagggag ggtgaattgg ttttgggcct ccaggatgcc
     8281 cttggcggcg atcccgaagc cgagaaaaac cggggacatc agcaggatcc gcatcaggtt
     8341 gacggcgaga tcctctgtgg caggatcgaa tccaggtgcg acgaggtttg tcagcgggcg
     8401 cgccagcagg aaggagatgc ctgcgagtat gacaatgccg agaccagtcc acgtcatgat
     8461 cgacgatgcc aggcgggacg cttgctccct gtcaccctga tcgatgaatc cggcaaagac
     8521 agggatgaag gcagctccga acgccccgga catcacggtc agaaaaagga ggtcaggaat
     8581 ccggaaggcg ctgacgtagg catccatctc gggaccggta ccgaattggt tggcgaggat
     8641 catctcgcgg ccaagaccca gcagccggct caaaataaag gcgatgctga cgacaatcgc
     8701 attcctcgcc atcgtggcgc cggcagatcg cgttgtgtcc tccgtggcgt tagtagatgt
     8761 ttctgctgat accgggttgc cgaagacctg ggacgcctgg ctcatacggg ggtggtctca
     8821 tcactaatgg cgtttgcacg tcggccacgg gaatacgacg ggatactcac aagcttgagc
     8881 gcatccgtgg gctttggtag actacattac tgcacccgtg tgaggggtgc tttgctatgt
     8941 tcagtattct atgatgtggt tcgtgattta tcacggtatg tatctgaaat gaggaaggtc
     9001 caacttggct aatagcaagt ctgccgagaa gcgaattcgt accagcgagc gtcgccgtct
     9061 gcacaaccgt ccctaccgct ctgctgcccg tactctggtg aagaaggccg agcttgcgat
     9121 tcaggccggt gaccaggccg ctgcacagac agcggttatt caggctgtct cgatgctgga
     9181 tcgcattgcg accaagggta ttgtgcaccg taacaatgcc gctcgtcgca aggcccggct
     9241 gatggcgaaa ttcaacgccg tcgcagccag cgcctaacag tatcgcgctc gcagtagaac
     9301 taatatgaat cgccccggag ggatgtatct cgacggggcg cttttggcgt tatgcggatt
     9361 tccgaaatgg caatctgtcg cgatcgggga cagggtcgtc atttaccggc gcgatgaggc
     9421 atctgtcggg acgcagcgat cccgagcacc gcatcgtaga gagcgtcaag cggatcccgc
     9481 agttcgccgc gcttcatctt ccggtcggcc gctgtggcaa tgtcaacccg acccagtgcg
     9541 gttcctgatg actgcccttg cagactctgc cgaatggaac tcatcctgcc cgcactagtc
     9601 acacctacgg ccttgccgac ctctgccgga tctcgccggc ccgcggttgc catgacagca
     9661 ccgagttcaa cgttctggct cagctgcgcc agcaacttcg caggatcctc cccggcctcc
     9721 agcaacttgt ccaactcgac cattgcgatt ccaacgttgc ccgcgacagc ggcatcgagg
     9781 aacgtgaaga tgcggtcgtc ggagccccga tccaccagct ctgaaacaag attgcgggtg
     9841 atcgttcctg gatgtgccgc aagcgccagc ttcgcaatct cgttcgagag catctccatg
     9901 tccggtggtc tgtcgaaagc cggattgcgg gatttctgca tccatgtctg tgggtagagc
     9961 aattccgcga ggagccgggc ggtctcctgg tcgatttccg agccgattgt cttggcggtg
    10021 ccctgaatcc acttcaggag ggccgggccc cggggtggat cgctaagatc gatggaggca
    10081 gtcggcggca aggccttttt tactgccgcc ggaagggact gaagcgaggg atcaaggaga
    10141 atgagcgtac tggcatcggg aaccgcggag aagagcgccg accagtcggg agtatttccg
    10201 ccgtccctgg cgccctgctt cccgaggcgc gcgatgaggt cctcgacgat cacgacacgt
    10261 cccgccgaga aaaagccgat gctcgatacg tccatgatga tctgacggat cgaaacagct
    10321 ttgccgtcca gcatggaagt gctatcgccc gacgaatcgc gctgcttccg gatcgtgttc
    10381 acacgggctc gagccatcgc gtagtccgga ccaaggatga tacagatcac gaagaggctc
    10441 ctgacggaga gtggtattag ggggaataaa gaccggaccc gctgatgccg cgcgcgatgc
    10501 ggagccttga acctgaatta acaggtgggt gccgcagctt gcgtgaacca cgatccacgc
    10561 tcgagcagct cccggctcac aaaacgttgg ctcaagacca catcaagcgc aacggcatcc
    10621 ggcaggaccg gccggacgtc cagagtagca tcttccagcg gtcccatcaa cgcactacgg
    10681 ccgtgcctga gcgggtatga tt
//
LOCUS       DASYWM010000164        43401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_532047, whole genome shotgun sequence.
ACCESSION   DASYWM010000164 DASYWM010000000
VERSION     DASYWM010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 43401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43401
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532047"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VGR29_06605"
     CDS             <1..706
                     /locus_tag="VGR29_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073300.1"
                     /translation="DKARKAVGSRASETVFFLAQHQPGGSDPVDLLRAEVTETLPDLA
                     PSYGRFFSAWRPAAVRAIIDETAKANAQFALVSNIPAVIPILGGFVAASADLLVLTKN
                     QVMMCYKIAAAHDQELGHQMAIVRELTPVVGAGFLWRTVAREAASFIPLAAGTIPKVI
                     IAFAGTMTVGRAADYYYRFGKKPPKDQMDQFLESATKMAKKLPIIGDNSDEEKVQEQK
                     ELVSGEMGKVDSASRS"
     gene            complement(779..2164)
                     /locus_tag="VGR29_06610"
     CDS             complement(779..2164)
                     /locus_tag="VGR29_06610"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV2073301.1"
                     /translation="MQNILIADDDAAIRQVLRDFLSDEGYEVQDVDSGASVLETMANG
                     DSAPKLILMDVRMPDKSGMDVLQELRKTNEGQLPVIIMTAFGGSGVAIEAMRLGAYDY
                     ITKPFDLDEVLLTVKRYFERLRLNDQVLELSALLDRNDPNDPLIGNSLAMQEVYKTIG
                     RVAHSDATVLITGETGTGKELIASILHRTSSYARGQLIKVNCAALPETLLESELFGHE
                     KGSFTGAMNQRKGRFEMANKGTIFLDEVGEMTLSTQKKLLRVLQEREFERVGGSVTVK
                     IDTRVIAATNKILAQEIEANRFREDLYYRLNVISIYLPPLRDRKDDIPLLVEHFVEKH
                     RYKAGAPVARVSQSAMDKLMGYEWPGNVRELENLIQRATVLAQGQVITEDHIHFHGTD
                     SRRLIDIGDRVRRGISLDDLLGEIEKTALEEALVATDDDRKEAASLLGMSLQELNKRI
                     RAANLETVPAK"
     gene            2288..3940
                     /gene="aroB"
                     /locus_tag="VGR29_06615"
     CDS             2288..3940
                     /gene="aroB"
                     /locus_tag="VGR29_06615"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV2073302.1"
                     /translation="MVTRLVLIGFSGTGKSTVSRLVAERLGWSLLDMDAEIERREGMS
                     IPDMFSARGEAAFRALEQAVFLEALDREEVVVATGGGAVCTEEIWRLLDADPSTVVVS
                     LDASPEILLERLTAHGRQAAGGETTKRPLLDSDDPLNRITLLKADRRPYYRRADVTIP
                     VGHRSPESTACDITELALLANGTASTVTLDLPQGTSSILVGRGQREEVADLLRSRWPK
                     AQRAWIAADAAVHKHHATWLTSLEITSGVSLHVRDVPSGESSKSISGLADLYDWMIGG
                     GVERGDVAIALGGGVTGDVMGFAAATVLRGIGLMQVPTTLLSMVDSSVGGKTGINHPG
                     GKNLIGAFYQPALVVVDPAFLVSLPKRELRSGFGEIIKHGVIQASTPGGENGFLLDVL
                     EANAASLLALEEPLTSWVIRQNVSLKASVVREDEKESRLRAILNFGHTIGHGVEAAGY
                     SLLHGEAVAVGMVAAMTIAVEKGLIPDPARERLVRLLEAYQLPTVADVDTGTVRAKMA
                     RDKKKASGKQQWVLPMQSGGVEIRTDVTETEMELALEAIAGH"
     gene            4102..5097
                     /locus_tag="VGR29_06620"
     CDS             4102..5097
                     /locus_tag="VGR29_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEV2073303.1"
                     /translation="MTPSPQVVVSASIAFDHIQQFGGSFRDHILEDKAHVISVSFLLD
                     SLKHQRGGVGGNMAYNLALLGVPVALVGAVGSDFGPYREVLEELGVDLTYIQQVDDDL
                     TASAYMNADMAGNQIASFYPGPGALTQQMDLTEIAGQARYGIVSAGDPVAMMLHSEQI
                     AKAGKLIFDPAQQIVILDGGQLTRGIDLAEIVIGNDYELGMIERKTGLSVEDISRKAP
                     MTVVTYGSQGSEIFVGGESVKIPTATPDPLTDPTGAGDAYRAGLVTGLLLEKDLPVVG
                     RLAALAATYPIEFHGTQEHRYTQGEFVDRFDAAFPDYEGSVTVDELGHFKAAVGS"
     gene            5197..6471
                     /gene="ahcY"
                     /locus_tag="VGR29_06625"
     CDS             5197..6471
                     /gene="ahcY"
                     /locus_tag="VGR29_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEV2073304.1"
                     /translation="MVTTSNTRDFDIKDPSQAAAGHRRIEWAAREMPVLANIRKQFER
                     DKPFEGIRVVCCAHVTTETANLVLTLKAGGADAVVCASNPLSTQDDVAAALVDEGVRV
                     YAIKGEDDETYMRHIHSAIAHGPHVLIDDGADVVTTIHTQYPELIPDILGATEETTTG
                     VIRERAMEADGVLKFPIVAVNDADTKHFFDNRYGTGQSTLDGIIRATNMLIAGKSVVV
                     AGYGWCGKGIAMRARGFGAKVTVTEIDPIRALEAAMDGFEVTTMENAAKNADLIITAT
                     GNINVVDRQHFEVMKDGAMICNAGHFNSEMNLKALAEMSGEGRKTLRPFVEEYTIGDK
                     GVIVLGEGRLINLAAAEGHPASVMDMSFANQALGAEYIVREGKNLENKVYRVPEEIDR
                     EIAALKLKAMGVTIDELTAQQAKYLVSWATED"
     gene            6570..7790
                     /gene="metK"
                     /locus_tag="VGR29_06630"
     CDS             6570..7790
                     /gene="metK"
                     /locus_tag="VGR29_06630"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV2073305.1"
                     /translation="MANTFDTSKNLLFTSESVTEGHPDKVCDQISDAVLDAVLAEDPD
                     ARVACETSVTTGLVLVFGEITTSAYVDIQSLVRNVVSNIGYTNSNHGFDASQCGVIVA
                     IKEQSPDIAGGVDNALEQRQQMADDEYDTIGAGDQGMMIGYAVNETPELMPLPISLAH
                     RVARRLAEARKSGEIPYLLPDGKSQVTVEYSDGKPVRVDTVLVSTQHLPEATNEQIRK
                     DVEEKVIRAVVPAELLDDQTKIVVNPSGRFVIGGPMSDAGLTGRKIIVDTYGGMARHG
                     GGAFSGKDATKVDRSAAYAARYVAKNVVAAGLADRFEVQLSYAIGVAHPVSFMCETFG
                     TGKVSDEVIERVVRENFDLRPAAIIDHLDLQRPIYRQTAAYGHFGRDDLDLPWERTDK
                     AETLAKAAGMVQTV"
     gene            8148..8501
                     /locus_tag="VGR29_06635"
     CDS             8148..8501
                     /locus_tag="VGR29_06635"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEV2073306.1"
                     /translation="MKFVIAVVQDYDCDKLLRAIAKENLGATKISSTGGFLRMGNATV
                     LMGVENRRVPEVIELIRKHCQSRVDVQLDATSPEYEEWFPAGVHEVTVGGAVVFTLSV
                     DRMVRIMPDAGGFAS"
     gene            8498..8734
                     /locus_tag="VGR29_06640"
     CDS             8498..8734
                     /locus_tag="VGR29_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073307.1"
                     /translation="MTQDRFRWLRRYWQGLLIAAAVSYGQLHDTGGTDVRAAQRHPYV
                     WMLPDGPAQPSPLETVGQYILTMPFDPEAERVWN"
     gene            complement(8767..9045)
                     /locus_tag="VGR29_06645"
     CDS             complement(8767..9045)
                     /locus_tag="VGR29_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073308.1"
                     /translation="MNSSMIGKIEKAHRYAQETDRVKFNFFDATVQGDNNAYQVKLTP
                     DGWDCTCNTFRTQSLGTCSHVMAMQLMLGKMLKDDVRYDKQTASVLAE"
     gene            complement(9436..10293)
                     /locus_tag="VGR29_06650"
     CDS             complement(9436..10293)
                     /locus_tag="VGR29_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2073309.1"
                     /translation="MANSKQKPITKKQLNQQALEASLESRWEDALKINDQILERFPRE
                     AEALNRKGRALIELRQLSAARDAYSEALKADPANMIARRNLQRLETLHNRPTGQPEGK
                     MTADSTIPKSTVFVEEIGKTWVDELANPAEPGQLAEVSPGDKLQLKVEGERLLVLSDD
                     GVQLGEIEMRVASRVIKLMGSGNEFEAYALGMSAQSLRIILRETFRHQDNEGLMTFPR
                     QIRATQDLMREREQLSLREEGDFDFGVEDEDVEDEAEDEEPDEVDEEASAYVGNAGVS
                     DMDDEEDAQ"
     gene            complement(10425..11807)
                     /gene="miaB"
                     /locus_tag="VGR29_06655"
     CDS             complement(10425..11807)
                     /gene="miaB"
                     /locus_tag="VGR29_06655"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEV2073310.1"
                     /translation="MSTKPIIQLVPESSAPAAVAPRRLPTNGKRFFIWTMGCQMNEAD
                     SQKVAAMLQEVGYERAPNEEEADIVVLNSCVVRQAAEDKVAGKLHSLARLKKAKPEMP
                     LVLTGCMVTRQQEKLKQTFPHVDLFFDPSDFDKLVQVVPELNDLEDDLAQLPHYYQPE
                     LATGKAGVSAFVPIIYGCNFVCSYCIVPYRRGKETSRPFSDIILEVERLVSNGVREVT
                     LLGQTVNAYGHDLHEKTTLAHLLEAVNNINGLDRLRFLTSHPKYMGDDVIHAIRDLDT
                     ACEHMNLPVQAGDNEVLRRMRRTYTRDLWMERIAYTREQVPGVTVATDIIVGFPGETD
                     EQFLQTYDMLEQTKCDKVHLAMYSPRPGTLSSRWEDDIPHEEKFRRHQAIEKLQEQLC
                     TGINGNRLGETYEVLVEGKSKGRWTGRTRGNTLVHFDDDRDVLGKMVDVRITKTSPWF
                     LLGEPSGEPH"
     gene            12080..13177
                     /locus_tag="VGR29_06660"
     CDS             12080..13177
                     /locus_tag="VGR29_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073311.1"
                     /translation="MKILTRPVFGRRYKLCQTLSAGVVALSLLAGGAAYSVAASPLQP
                     ATMVSVYAQNVDWMSSEAAASLDIGIDVLVPSWIPEPFSGVSPSITASDGYYQLYWMV
                     PGGSPTFLYIEGVVGGALPAGSPADLNKQLNINATVQGWDAIQDTGIPAGGSTPIYDQ
                     VWWIANGVLYTVSSNNMTGSDSLSLANSLVVLEAPELPAPTEAPYEPPTEPPYVPQTE
                     SPYIPPAESVPVAEVPAPSGQVVTEEVSTEQVSSEELSNEQVQSDGNTTDPASTDAET
                     VSEPNALTAPTDEPVTDTTVNVGGTSETLDASGGTTTAQTSALQGPWSPGRYDSAVPS
                     DGTNGPLPPLIGGDGTGGLYDTALPSIRIRP"
     gene            complement(13196..15349)
                     /locus_tag="VGR29_06665"
     CDS             complement(13196..15349)
                     /locus_tag="VGR29_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2073312.1"
                     /translation="MSSERFKNWARARMSPTTWDRASATLAGIGHAMAELGSNTRHWA
                     SIGSDHVTSMRAARIEPWRLVFAFTIVIVPALLLASITPHLGPEIPIVFFLLSVALAT
                     YVADWVGGLTSALTSLLVLDLFFLGIRGRFDFWDLRNQVWVLLVFGLGSALFVSLFEH
                     LKYDRASAKLEASAMRAANTAFNAVEIAAASRPAGDSEAFVSVLQSILTAMVRVNRAS
                     AGALYLVDAPNATLVQAATYGDTSDSSLSPADDPTRTIPIDYGFAGRVASERRPIAIA
                     DIESDPEFEDVLNTNPHVRAVVGVPIFGPTDALAGVAWVGLYVPYRFSQTAIARLQAL
                     AHRTVAFMESARLADAQEELLDRVQDNHRRLQSVIQTMPEAVMVARPPHGLIVTFNAA
                     AQRMFGLQAHTSQLVSRRVDQLAVTSEPVDAVPVMPMVQAMTEGITVTGVELVVRTPG
                     GTMLPVVASAAPLKTDEGDIDAVVGVFQDVAPLKEAERLRDEFISVVSHELRSPLTPI
                     RGFAQIMARELDREGGHEQHVAWLNTLQQHTDRLTRLVDDLLDVSRMRAGRLRVLRED
                     VDVVELCRALVESKGATQSTHNVILDTDLTSLTARMDGDRVHQIVDNLVSNAIKYTDG
                     GTVTVGLALQPDGDIQISVSDEGSGIPQRERESLFTAFYRARSANESAVPGLGLGLYI
                     VRELVLAHNGTITVDEAATGGARFTVELPRNMNVL"
     gene            15545..16612
                     /locus_tag="VGR29_06670"
     CDS             15545..16612
                     /locus_tag="VGR29_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2073313.1"
                     /translation="MSNQLKQQTDGLVIDENEQVFDVNIIGAGPTGLFAAFYAGLRGM
                     SVQIIDSLEEAGGQCAAMYPEKYIYDVIGYPKVLAKDFVAACLEQAMRSDPTIRLSEQ
                     VQDLIQLDDGSFAMKTSKGVRRSKAIIIAAGVGAFEPTRLTASGVVELEGNGVHYFAK
                     RVDDFRDKKVVIVGGGDSAVDWAATLEPIAESVNLIHRSKFRAHEASVQELEESTVVL
                     HYPGYEVTTVRAGDDGRISGLTFTHQDGTSTDLECDELICAIGFKADPGPLRSWGFEL
                     KRNQIAVDIQTMQTSIPGVFAAGDIATYPAKFKLIATGAAEAVTAVNHALVHIRPDAR
                     LDGGHSTTIMEKRAKVVEAAV"
     gene            complement(16751..17788)
                     /gene="trpS"
                     /locus_tag="VGR29_06675"
     CDS             complement(16751..17788)
                     /gene="trpS"
                     /locus_tag="VGR29_06675"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV2073314.1"
                     /translation="MAATVDKTSSTETRRSGPRAFSGIQPSGNLHLGNYLGAIRNWVL
                     QQDEYDSVFSIVDLHALSLATTRESMQSNIRNLANTIVAAGVDPGKAVLFVQSDVREH
                     AELCWLLGSVTQFGELRRMTQFKDKAGEHEEGVSAALFVYPVLQVADIILYDANKVPV
                     GEDQKQHIELTRDVAYRFNQRYGETFVLPEPDIKTTGARIMALDDPTKKMSKSSPNQN
                     SYIALNDEPDVIRKKIRRAVTDSGSEIVAGRDKPALTNLMSIYAILSDNSLEGVSEKF
                     IGKGYGPFKQELGEVVVDALAPIQRRLRELEESPEIVDAILAEGAEEARTIASAKMRL
                     VRERMGLGGAG"
     gene            17925..19589
                     /locus_tag="VGR29_06680"
     CDS             17925..19589
                     /locus_tag="VGR29_06680"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEV2073315.1"
                     /translation="MIETMFLHRRHARRYRQIVRVLLSHGLGGLIAPFDPRGRHDAEQ
                     EDVQPAGRVTARRARHLHRALEELGPTFVKLGQILSTRADLLPPVYIHELEQLQDAVA
                     PDDPAAIRRIVEQELGGSIESLYTSFSLVPLAAASIGEVHAATLHDGSEVVVKVQRSG
                     VAALIQQDLSILADVARIAEQRSAKLRQHDVSGLIREFSWTIRAELDYQHEARNLERF
                     HRAFASNPAIRIPAVVRDLTTSRVLTMERIDGIPIDDVQSLRGAGHDTIKLIESVIQM
                     LAEGILDIGLFHADPHPGNFVVDQDGALVVYDFGMVGSIDERLRERLLLLTLACAERD
                     SSRIVDEITQLGAVQPGWDRQAMERDISYLVGQYVGAPLESVPLPMIVGDVMAMMRRH
                     RLRLPSELALLAKTATMAESLARTLDPQLNVIELVDPTIRHAMKRFSSLGFWKDRLRA
                     RPLEIALLGASLPGHIQRILSRIDRNDLTFHVHYEELPETIQGLNSMVNRLALAILSA
                     AGMIGFAMLYLAMQPTFGSWHGWLFGTVFLAIAVMTLTVLVRIWRS"
     gene            complement(19687..20037)
                     /locus_tag="VGR29_06685"
     CDS             complement(19687..20037)
                     /locus_tag="VGR29_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2073316.1"
                     /translation="MATTSRTEDVGCPVARTAEIIGNKWTPLIVRDLAHGQKRFSELE
                     RSLRGISPKTLSERLKKLEDCEVVDRRCFAEVPPRVEYTLTSKGYALLPVIDSMREFG
                     RDWLSDDDCGPEEA"
     gene            complement(20633..21337)
                     /locus_tag="VGR29_06690"
     CDS             complement(20633..21337)
                     /locus_tag="VGR29_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /protein_id="HEV2073317.1"
                     /translation="MNVAQGDAQRGSANTRWSAVTKRDVSAEGQFVFAVRTTYIFCRP
                     GCPAKTPRRENAEFYDTPARAIQEGYRPCKRCNPSGTSTRQRHLEVIAEACESIAAAD
                     TVPTLAHLASSAGMGPYHFHRTFKSITGLTPGQYARSHRAARVREELPTSPSVAQAVY
                     ETGYQSSGRFYASTDMTIGITPSAYRDSGPGERIRFAIADTSLGLRDCSHRQRDLLDR
                     VWGGSRDFVRAGARTV"
     gene            21541..21882
                     /locus_tag="VGR29_06695"
     CDS             21541..21882
                     /locus_tag="VGR29_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV2073318.1"
                     /translation="MIRLIAYALSAVIAALATATISERLLTFDTPQSVIIFGLVMGVI
                     DAFIKPIVRLLSFPITCLTFGLFSLVINAALFGLGSFLVPGIEVSWVGAVLGSILVSI
                     ASGVIFSVLDE"
     gene            21875..22486
                     /locus_tag="VGR29_06700"
     CDS             21875..22486
                     /locus_tag="VGR29_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV2073319.1"
                     /translation="MSRRSQAAGPTTRERNFDAPLTIGVISDTHIYPHSSRELHPALI
                     RFFRRANVGLMVHLGDVNSRPVLDEIADIAPLIAVHGNNDDEELQVVLPRTTRFSVGR
                     FTFGVIHGHGGRSARDEAIKRWVGKVDCVLFGHSHKPLLEKINETVLFNPGSAVERRW
                     HPHFGVGLITIEEDRFTPDLVVFTHPEHLDNVQVGTDETGSRM"
     gene            22531..23625
                     /locus_tag="VGR29_06705"
     CDS             22531..23625
                     /locus_tag="VGR29_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEV2073320.1"
                     /translation="MDPRLDQGRWLRIPGPTPLHPDVVQALTHEMVPHRGPEVQETMA
                     RIQPIAKRVHGTEKGLVLLWAGTGTTGFEASIVNLLAPGDHVVVTVAGDFGGRYARVA
                     EALQLHVHRLEVEWGEAVTPEMLKAAIAAHGDVKAVLITHNETSTGVTQPLDDLSSVA
                     RDAGALVLVDAVSSAAALPINMDENGLDWVLSGAQKAWMCPPGLMISALSERALEVAR
                     HQKGYPRATWDAIAMAEASAQNYHPTTGPLTALFALDAALQAVQTEGIDQLFERHKRL
                     GTHLREGMERIGIEILADPRYVSDSVTAFIPPSGMSAATFRDKVRDASGVEIAVGQGK
                     LADSVNRIGHMGWVAQPELDATLEALEAAK"
     gene            23789..25540
                     /gene="rpsA"
                     /locus_tag="VGR29_06710"
     CDS             23789..25540
                     /gene="rpsA"
                     /locus_tag="VGR29_06710"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV2073321.1"
                     /translation="MDGTDVREQEAQVNGDEQEQTMDASAQTTANGVELDEELDGASL
                     MEQFLSDPSHDYKTLKYGDVLEGIIMHVDRDELLVDIGSKAEGIVPSKEYSSLSTEEK
                     DKLQVGDNILVFVVQPENPEGHPVVSIDRARQEKSWRRLQELHEANEVIEAEVTNYNK
                     GGLLVNLDGVRGFVPASQVSEIRGGDEAAKQADMARLIGSSLPLKVIEINRHRNRLIL
                     SERQAVQERRDVMKEQLIQELHEGEIRKGRVSSICDFGAFVDIGGADGLVHLSELSWS
                     RVRHPSELLRIGEEVDVYVLGINATEKKIALSIKRTQAEPWSRVANSYEVGQLVKGTV
                     TQLANFGAFARIEDGIEGLIHVSELADERIQHPKQVVQEGQDLLLRIIRIDPQRRRMG
                     LSLRRALDTPDAELQAVFGEEILEERDALAQRIRVSLDAEGLTGTRQDEPAPSEVPAA
                     RTETGTAEPLDVDAATPALGEAQVAAFEERSRRAEQAAQQRQQRQRARQQQQQQEGGA
                     NFEDESMSAMAMAFAMAGADADLLAGADEQAAAAAAATESEDTVAPAAEDGGDATEVT
                     EKDVLQDTVGDEESSEG"
     gene            25817..28300
                     /locus_tag="VGR29_06715"
     CDS             25817..28300
                     /locus_tag="VGR29_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV2073322.1"
                     /translation="MAEKFDKFTDRARKVLTLAQEEAQRFNHNYIGTEHLLLGLVREG
                     DGVAAKVLANMGVQLPKVRSAVEFIIGRGETMVLAEIGLTPRAKKVIELAVDEARRLN
                     HHYIGTEHLLLGLVREGEGIAAGVLESLGVNLEKVRTQVMQVVSQGGSSPSSAGPSGK
                     GQAQTKTPYLDSLGFDLTEAARSDKLGPLVGRSTEIDRVMQILSRRTKNNPALIGEPG
                     VGKTAIVEGLAQRIVSGDVPDQLQNKRLVALDIGALVAGTKYRGEFEERLKKIVNEVK
                     ETKSILFIDELHTLVGAGAAEGAVDAANILKPALSRGELQTIGATTLDEYRKHIERDA
                     ALERRFQPVHVQEPSIEETIQILKGVRQLYEVHHKLKISDEALDAAANLAARYVPDRF
                     MPDKAIDLIDEASSRVRMQRSAAPPTLKDAMTGLGSLQNELEDAVNSREFELAAELRD
                     RERKLRDRIETMERDRRETQAAEEVYVTEEDVAGVVSMWTGIPLVRIAGEESERLLKM
                     EEVLHEKIVGQGEAITTISKAVRRARAGIKDPRRPIGSFIFLGPTGVGKSYLARMLAE
                     FIFGSETNLIKIDMSEFMERHSVARLVGAPPGYVGYEEGGQLTEAVRRKSYSVILLDE
                     IEKAHPEAFNMLLQIMEDGSLADAKGRRVDFRNTIIIMTSNIGAEEISRDMTMGFVSR
                     QDEEGEKSREHGLMKDKVMQRLKQTFRPEFLNRIDATIVFHSLTKLEIRQIVDLEMER
                     VREQLKEQGITLQITDGAKDFIGKEGYDHTYGARPLRRVIQNRIEDPLAEGLLDQRFP
                     SGTTVKADVKDDEITLESVETEANELIPV"
     gene            28377..29747
                     /gene="radA"
                     /locus_tag="VGR29_06720"
     CDS             28377..29747
                     /gene="radA"
                     /locus_tag="VGR29_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV2073323.1"
                     /translation="MAKTRTTWICQQCGTASPAYLGRCPGCGQWNTMVETIDERSSPK
                     AAARRRVSGTSALKPLNQISPEGQARTPVGIAEFNRVLGGGLVPGSLVLVGGDPGIGK
                     STLILQAAGALAEPNAPVVYVSAEESAQQIKLRADRLRVRSDDILVLSETNLDEILAT
                     AEAAKPGLLVVDSIQTVMLDEITSAAGSVSQVRECTSRLMQWAKPRNIPVFIIGHVTK
                     EGTIAGPRVLEHMVDAVLYLEGERFHQYRILRAVKNRFGSTDEVGVFEMIGDGLREVR
                     NPSEAFLEERSSIAPGSTVAVTMEGSRPILVEVQALATPSSFSMPRRSANGLDTNRLQ
                     LLVAVLQKRVGLGLGTQDVYANVVGGLRIMEPAADLAVAVAVASSLRDRPVEPSTVCI
                     GEIGLSGELRSVNHLERRVQEASRLGFRRAIVPERSANLPAVSKLGIEIIPVSTAAEA
                     IEAGSP"
     gene            complement(29748..30896)
                     /gene="murG"
                     /locus_tag="VGR29_06725"
     CDS             complement(29748..30896)
                     /gene="murG"
                     /locus_tag="VGR29_06725"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEV2073324.1"
                     /translation="MTSNQKPLRLLIAAGGTGGHVLPAIAVIEELKSREIPAEILWLG
                     SYKGVERDIAHSHGIAFAPLQTGKIRRYLSLETITDLFRIPVGIAQAWIKARAFKPDV
                     IFSTGGNVSVPTVVGGSRVAPVLTHEQTAQVGIANKIAARFSERFAASYDETAKIARR
                     YHHHVVVTGNPVRASLTEGTKMKAWEHFGLSPNLPVIFVTGGARGASPINQRVEKLLP
                     GLLEKTQIVHQSGPSVANEDAARLRSLRDSWPQHVRERYVVQEFLGPEIADLYAITDL
                     VIARAGAGTVAELAFLGKPSILVPLPGTWGDEQRKNANLLAGADGATVIEQDDATPER
                     LEEEIVALLADPMRRHAMGANARRIARPDAAARLVDELLTLVDYSERS"
     gene            31132..31662
                     /gene="lspA"
                     /locus_tag="VGR29_06730"
     CDS             31132..31662
                     /gene="lspA"
                     /locus_tag="VGR29_06730"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV2073325.1"
                     /translation="MSDAPGRRCASPRPIFPLLVLLPILIVVLDQLLKAVMVQWLGPE
                     STLHRWELAGSSLAFEYLENRGAAFGILPNQTELLTVLSTGIAVAGIALMWRESRTHP
                     MMAIAIGMVVGGAVGNIIDRIRLGYVVDFIAVGVWPKFNLADAMITMGVLLLLWSTVQ
                     EERRDTRSNQGINTHE"
     gene            31655..32653
                     /locus_tag="VGR29_06735"
     CDS             31655..32653
                     /locus_tag="VGR29_06735"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV2073326.1"
                     /translation="MNERTPGTLLGVDSEDIAEIIELRPDPSHRNMRLDRYVAGELPD
                     LSRTYLQSLIDEGMLLVDGRTRRAAFKMTPGEVVTVALPVPRQFDLEPQDIPLDVLYE
                     DADILVINKSSGMVVHPAPGHPKDTLVNAVLYHAPEISIQGSTRPGIVHRLDKDTSGV
                     MVLAKSDRAQTSLVEQWLERSVVKHYAALVAGIIEEEEGTIDAPIGRDQVNRQRMGTS
                     RAGREAITHFHVQERFDDATLLDVRIETGRTHQIRVHLAFIGHPVVGDAIYGNKVSER
                     IARDHGATRQFLHASSLTFKLPGTEEMRTFSAPLPHELEELLERFREGSESDVAEV"
     gene            32637..33965
                     /locus_tag="VGR29_06740"
     CDS             32637..33965
                     /locus_tag="VGR29_06740"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEV2073327.1"
                     /translation="MLQRYEEIHDRIERWYRAALEVVEPKAAVARAMAWDGERLVVDG
                     QSIPVDMNVRVFVVAIGKAAVAMAEGAVEGLGDRIDDGIVLTKYGHLGAGIRGFHCFE
                     AGHPIPDASGEQATRSIIDAFEGLTSNDIVLALISGGGSALLELPREPLTLQDLQQTT
                     EVLMHAGAGIHDLNAVRSVLSQVKGGGLRRHIGAARCVSLLLSDVLGNDPQVIASGPT
                     VLVHRSFAEAQAVVSRFGVEGRLPESVRLVLAGEDRIDSTPDSSHDVWTVIADNAAMV
                     NELQTVVEAEGLSARLQWQAYDGDAANLGRKMVEDARASDDTWDVLLGGGEATVEVTG
                     QGSGGRNTEAVLVSAMALEQQGGDWVVASLASDGDDGSADAAGAIADPETVARGRLAG
                     VEPSTSLANSDSASYFRKAGGLVVTGPSGTNVNDVYIAVRIRPTFDQGEN"
     gene            33965..34903
                     /locus_tag="VGR29_06745"
     CDS             33965..34903
                     /locus_tag="VGR29_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEV2073328.1"
                     /translation="MEKRPLMLDVDTGVDDAAAIALAIGLKSEPVAISTVAGNVPIDA
                     STENTLRVLSLLDKGDVPVYRGASRPLTATYQDAAHVHGSNGLGGVELPPARATQCDY
                     TGPEAIVRMAERYAGELTLVTVGPLTNLAIALSLRPAITRQITRVVIMGGAYFHPGNI
                     TPFSEFNLYVDPDAAEQVFNAEWDDVTAIGLDVTHQTALTRNMWEAIPADASGSAGLI
                     RAISERTFEERARSGFFLHDPLAVAVALDSSLITGQHKAVTMDLLGDTRGQSNVTGVG
                     NVLVATEVRADVFVRRFCEALNLPYVEGTEGLANAE"
     gene            complement(34957..35910)
                     /locus_tag="VGR29_06750"
     CDS             complement(34957..35910)
                     /locus_tag="VGR29_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV2073329.1"
                     /translation="MKRELDLVEKQLQESARVDYPIISTMLQDIVKGGGKRLRPLLLL
                     LATRSFSAPGEHAIIAAAGVELLHTASLIHDDNIDRAALRRGNPTLNTELSSGAVILI
                     GDYLFAQSAILAAATGVPRVVMIFATSLADICDGQLRETLEAHDLGQTRESYEQRIYG
                     KTAALFAGAAEMGAVIGEAPEYAIQELRRFGTELGMAFQIVDDILDLRERTDTIGKPA
                     GNDIRQGVVTLPVMMFAEGLSSETDTRRTIERIISGDETNDARIDGVVAQIRDSGALE
                     RAEDVARAYVEQARKRLEVIPDDETRRYFNDMLDLAVVRQS"
     gene            36175..37989
                     /locus_tag="VGR29_06755"
     CDS             36175..37989
                     /locus_tag="VGR29_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEV2073330.1"
                     /translation="MSNYPRAPIAPLAASHGPVNSEPERRWPGVRWSHSLILPVALLL
                     ILITLAVAEPRKAFLELEVASNVSGEAIPGAAVTIGNTSYIADKDGKIVVERPERGAS
                     LSAASEGYEPLVAKFASSEQRSVTLQLKPSIVQGVLRDGGSGEAIADADVALLDASGN
                     VVAETRTDSSGAYLFKDLPAHSLLRIDAGEYGVIQKEVGQRAVVNLDLDRSVYRGKVL
                     DDTGAPLQGALVRSGDSRVVTGADGEFTLEGVGQGAEVRITAAGFRSMTRDIGSEGLG
                     DIALEPQMIKAVYASWALLADPDGLDSLIDIANTTEINAIVIDIKQDTIYYDSQVEFF
                     RRAGTVAPIYDAREVVRRLEENNIYPIARLVVFQDPLVAEAYPQFAVQDSTGKLWRNE
                     MGVAWVSAFNEELWNANIELAVEAIELGFAEVQYDYVRFPSDGDLSTADFGREYTAQA
                     REDAITEFMRRSYEAVHHAGGLLAADLFGFVTIVDDEQYIGQRFSRLLPYLDYVCMMI
                     YPSHFSEGNIASAAGHPNDFPYETIYESLERAEQIAPGAKKKFRPWLQDFSYGSMREY
                     GAADVQAQIDAAEDYGASGWMLWGDPFNVTVEALRHDD"
     gene            37982..38902
                     /locus_tag="VGR29_06760"
     CDS             37982..38902
                     /locus_tag="VGR29_06760"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEV2073331.1"
                     /translation="MIEAHDLSSARVEALRRARVNRWVRLLRPVQWAKNGVVLAALVF
                     GRVYEDPAKVWASLGAVLAFCLISSAGYILNDWLDVERDRHHPLKRARPLASGAIRPR
                     SALILGGALATTALISSLLISPSLALVISVYALLMSAYSVWLKRVVIIDVFVIAAGFL
                     LRAVAGGVAISVPISTWLMLCTVLLALFLGFCKRRNEILTLQERAQLHRVSLRGYTPE
                     ILDQFILLTASSTLMAYAMYTFTAESVPTNHAMMITVPIVAFALFRYLYLVYGRALGG
                     APEVLLFRDAPLLGAIVLWACAVFIVFWLG"
     gene            39146..39622
                     /locus_tag="VGR29_06765"
     CDS             39146..39622
                     /locus_tag="VGR29_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV2073332.1"
                     /translation="MIVVRRVRPREQARRQTEIDAMYRSLLAGHRAVGTGRRRTWRPS
                     VEVYETAETLEVVAEIAGMQGDDIDIVLEGDVLMIQGTRPDPAPCDQRTYHIAHIEYG
                     AFATELRIPFAVDTDSATASYENGFLRITLPRTKGRTIVATRAGSNSDTADEQRDA"
     gene            39619..42195
                     /gene="lon"
                     /locus_tag="VGR29_06770"
     CDS             39619..42195
                     /gene="lon"
                     /locus_tag="VGR29_06770"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV2073333.1"
                     /translation="MSNPDEKMTNTNINDETQDDQEHHELPEAVLPEAENDGNDQAGP
                     VEVVAEVVGDTDASPELPEEEILPLLPLRGTVVLPMTLVPLAAAQERSLRLIDDVMAG
                     ERTVAMVMQKDAQLEGAGPDDILSIGTITTIHQMMRVPDGSVRLAVQGVERMEVIEVI
                     QEEPYLKARIRRLPEDLEDSVEVQALMRNTLELFQRLVALVPHLPDELVTAALNVDDD
                     ARHLVYLVASNLRMEPEERQSLLELNSVTEKLQSLNAFLTKELDVLELGRKIQTDVQE
                     EMSKSQREYFLREQMKAIQKELGESSETEAEVSELRQKIEESGMPEEAAKEANRELDR
                     LSKLPPAAAEYGVIKTYLDWLTSLPWNVSTENEIDIKKTREILDADHYGLDKIKDRIL
                     EYLAVRKLKLDQATDDGETQAAHREPILCFVGPPGVGKTSLAQSIARGLGREMTRMSL
                     GGVRDESEIRGHRRTYVGAMPGRIIQAIRRAGSNDPVFVLDEVDKLGNDWRGDPSSAL
                     LEVLDPEQNNDFRDHYLDVPFDLSKVMFIATANMLDSIPAPLRDRMEIVELSSYTDDE
                     KLQIAKRYLVPKQMKANGLGGQEIDWDDDALMSIIQHYTRESGVRNLEREIGTAARKI
                     ATIVAKGDNAPETIGKREIRDYLGRPRYFYEEREQRTAQPGVAIGVGVNSFGGDIMFI
                     EATRMPGKGSLTVTGQLGDVMKESATAAFSFVRSKTRELGIDPDFYKDSDIHIHVPAG
                     AIPKDGPSAGVAMTTALVSLLAGVSVRDDVAMTGEITLRGQVLPVGGIKEKALGARRA
                     GITTFILPRRNEVDLDDLPEELKDEMIFVLADTLDEALQTSMPEEFHGVRAASTPVAI
                     AS"
     gene            complement(42274..43017)
                     /locus_tag="VGR29_06775"
     CDS             complement(42274..43017)
                     /locus_tag="VGR29_06775"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV2073334.1"
                     /translation="MKPVIGISPSPSESTFDHGHFRRYALGDTYTRAIIAAGGVPVIL
                     PLHTGAIDETLDAVDGIVFSGGGDIDPGYWNEEKHPAADGFDDERDAFELQAIPKVVE
                     RDIPMLGICRGIQTVNVALGGTLIQDIPDQLPGAQQHRQHREGKMRDARSHTITITEG
                     DNLLYRIHGDTKMQTNSFHHQAVKDPAEELEVVAITPDGVVEAMWHPRMTFGLAVQWH
                     PEMLAEEYPDQAAIFEALVRAAADKKAGK"
BASE COUNT         8817 a        12217 c        13474 g         8893 t
ORIGIN      
        1 cgacaaggct cggaaggccg tcggctcgcg ggccagcgag accgtcttct tcctggccca
       61 gcaccagcca ggagggagcg atccggtcga cttgcttcgg gctgaggtca cggagacctt
      121 gcccgatctc gcgccctcgt acgggcggtt cttctcggca tggcgtcctg ccgctgtgcg
      181 ggcaatcatc gacgagacag ccaaggcaaa tgcgcaattt gcgctggttt ccaacatccc
      241 tgccgtcatc ccgattcttg gtggattcgt ggccgccagc gcggatctgc tggtactcac
      301 caagaatcag gtgatgatgt gctacaagat cgcagcggcc cacgatcagg aactggggca
      361 tcagatggcc atcgtgcgcg agctgacacc cgtggtgggt gccgggttcc tctggaggac
      421 ggtggcccgc gaggcggcct cgttcattcc cctcgccgca gggacgatac cgaaggtgat
      481 tattgccttc gcagggacga tgacggtagg tcgagccgcg gattactact atcggttcgg
      541 gaagaaacca ccgaaggacc agatggatca attcctggag agtgcgacaa agatggccaa
      601 gaagcttccg atcattggag acaactcaga tgaggagaag gtgcaggagc aaaaggagct
      661 ggtatcgggc gagatgggca aagtcgattc ggccagtaga tcgtagcgtg atggtaggaa
      721 cggcccgcac ctcggaaggt gtgggccgtt ccagtttctg gcggtcttga ccgggttgtt
      781 atttcgccgg aacagtctca aggttggcgg ccctgatccg cttgttcagt tcctgcaagg
      841 acattccaag aagtgaagcc gcctctttcc ggtcgtcatc ggtggcgacc agggcctctt
      901 caagagcggt tttttcgatc tcaccgagaa gatcatcaag cgaaatgcct cgacgaacgc
      961 ggtctccgat gtcgatcagc cgacggctgt ccgtaccgtg gaagtgaatg tgatcctctg
     1021 tgatcacctg cccctgagcc agcacggtcg ctcgctggat aaggttctcc aactcgcgga
     1081 catttcccgg ccactcgtag cccatcagtt tatccatggc gctctggctc acccgtgcaa
     1141 ctggtgcgcc tgccttgtac cgatgtttct cgacgaaatg ctcgacaagg agcgggatgt
     1201 cgtccttgcg gtcgcgcagg ggcggaagat agatggaaat cacgttgagg cgatagtaga
     1261 ggtcctcgcg gaagcgattg gcttcgattt cctgcgccag gatcttgttc gtcgctgcaa
     1321 tcacgcgtgt gtcgatcttg accgtgacgg acccgccgac gcgctcgaat tctcgttcct
     1381 ggagcacgcg gagcaacttc ttctgggtcg agagtgtcat ttccccgacc tcatcgagga
     1441 agatggtgcc cttgttcgcc atctcgaagc gccccttacg ctgattcatc gcgccagtga
     1501 acgatccttt ttcatgcccg aagagttcgc tctccagcaa tgtctccggc agggccgcgc
     1561 agttgacctt gatcagctgg ccgcgcgcat agcttgatgt ccggtggaga atgctggcga
     1621 tgagttcctt gccggtgcca gtttctccgg tgatcaggac cgttgcatcg gagtgagcta
     1681 cgcggccgat ggtcttgtag acctcctgca ttgcgaggct attgccgata agcggatcat
     1741 tggggtcgtt ccggtcgagg agggccgaga gctcgagtac ctgatcattg aggcgaaggc
     1801 gttcgaaata ccgcttcacc gtgaggagga cttcatcaag atcaaagggc ttggtgatgt
     1861 agtcgtaggc gccgaggcgc atggcttcga tggcaacacc tgagccaccg aacgccgtca
     1921 tgatgatgac gggaagctgg ccctcattgg tcttgcggag ctcctggagc acgtccatgc
     1981 cgctcttgtc cggcatccgg acatccatca ggatcagttt cggggctgag tcaccattgg
     2041 ccatggtttc gagaacgctc gcacccgagt cgacgtcctg aacctcataa ccttcgtcgg
     2101 agagaaaatc cctcagcacc tgccggatgg cggcatcatc atcggcgatg agaatattct
     2161 gcatatgtcg cgtcaagccc ttctggatcg catgaagcgt ctttgcacgg gtgcagccgc
     2221 atcagaatac atagtataga gtgccggaca ccttgagaga ggtcagcgga aacaggagtt
     2281 gagaagtatg gtcacccgcc tcgtcctgat cgggttcagc ggaaccggga agagcaccgt
     2341 gagccgtctg gttgctgagc ggttggggtg gtcattgctc gatatggacg ccgagatcga
     2401 gcggcgcgaa ggcatgtcga ttccggatat gttttcagcc aggggagagg cggcgttccg
     2461 ggcgctcgag caagcggtat tcctggaggc actcgaccgg gaagaggtcg tcgttgccac
     2521 ggggggcgga gcagtgtgta cggaggagat ctggcgcctt ctggatgcag acccctcgac
     2581 cgttgtggtc agcctggatg catcacccga gatccttctt gagcgtctaa cggctcatgg
     2641 tcggcaggca gccggtggcg aaacgacgaa acggccattg cttgattcgg acgacccact
     2701 caaccggatc acgctcctga aggccgaccg aaggccgtat taccggcgtg cggacgtcac
     2761 cattccggtg ggacatcggt cgcctgaatc gaccgcatgc gacatcaccg aacttgccct
     2821 gctggcgaac ggtacggctt caaccgtgac cctggacctg cctcaaggga cgtcgtccat
     2881 tctggtggga aggggccagc gtgaagaggt ggcggatcta ctgcggagcc ggtggcccaa
     2941 ggcacaacgg gcctggatag ccgccgatgc ggcggtgcac aagcatcatg caacatggct
     3001 cacgtcgttg gaaatcactt cgggcgtgtc actgcatgtg cgcgatgtgc catctggaga
     3061 gagcagcaag agtataagtg ggctcgccga tctatacgac tggatgatcg gtggaggagt
     3121 ggagcgggga gatgtcgcca ttgcactcgg tggtggagtg acgggtgacg tcatgggctt
     3181 cgctgcggcg acggttctgc gcggcatcgg ccttatgcag gtaccgacca cgctcctttc
     3241 gatggtggat tccagcgtcg gcggaaagac tggcatcaat catccaggcg gaaagaatct
     3301 cattggtgcg ttctatcagc cggcactggt cgtcgtggat cccgcattcc tggtctcgtt
     3361 gccgaaacgg gagctgcgtt ccggattcgg cgagatcatc aagcacggcg tgatccaggc
     3421 ctccaccccg ggtggcgaga acggttttct gctggacgtg cttgaggcaa acgctgcctc
     3481 gctcctcgcg ctggaagagc ctctcacgtc atgggtcatt cgccagaacg tctcgctcaa
     3541 ggcgtcggtt gttcgcgagg atgagaagga gtctcgcctg cgggcgatcc tgaacttcgg
     3601 gcatacgatt ggccatggcg tcgaggcggc cggatactcc ttgctgcacg gtgaggcggt
     3661 tgccgtcggc atggtggcgg cgatgacgat tgctgtcgaa aagggactca taccggaccc
     3721 ggcacgggaa cgtcttgttc gcctgctgga ggcgtatcag ttgccgaccg tggcagacgt
     3781 cgataccggc actgtgcggg ccaagatggc gcgcgacaag aaaaaggcaa gcggcaaaca
     3841 gcagtgggtg ctaccgatgc agtcgggagg cgtcgagatt cgaacggacg ttacggagac
     3901 cgagatggaa cttgccctcg aagcaatcgc cggacactga tgcacgagtg aaaccggcgg
     3961 gcgctgtgct atgctctcct gtgatgtcag gtggtcgcgc gatcaccggc gttttgttgt
     4021 gtcccacagg acactccttg cctgcagcgc cagcgagccg gtgagcaagc tccaattgtc
     4081 acggacagga gtacaaacag cgtgacaccc tcccctcagg tcgtggtcag tgcgtccatc
     4141 gcattcgacc acatccagca gttcggtggt tccttccggg atcacatcct cgaagacaag
     4201 gcacacgtca tttcggtcag tttcctgctt gactcgctga agcatcagcg gggtggggtg
     4261 ggcggcaata tggcctataa cctcgccttg ttgggcgttc ccgttgcact cgtcggagct
     4321 gtcgggtcgg attttggccc gtaccgtgag gtgctcgagg aactgggagt cgatctcaca
     4381 tacatacagc aggtcgatga cgatctcacc gcaagcgcct atatgaatgc cgatatggct
     4441 ggaaaccaga tcgcctcgtt ctatccgggc ccgggtgcat tgacccagca gatggacctg
     4501 acagagatcg cggggcaggc acggtacggc atcgtcagcg ccggagatcc ggtcgcaatg
     4561 atgctgcact ccgagcagat tgccaaggcc ggcaagctca tcttcgatcc cgcgcagcag
     4621 atcgtcatcc ttgatggggg tcagctgaca aggggcatcg atctcgccga gatcgtgatc
     4681 gggaatgact acgagctcgg catgatcgag cggaagaccg ggctctccgt cgaggacatc
     4741 agcaggaagg cgccaatgac cgtggtgacc tacgggagcc aggggtcgga aattttcgtt
     4801 ggcggtgaat cggtgaagat cccgacagcg acacccgatc ccctcaccga tcccaccgga
     4861 gccggagacg cctatcgtgc cgggcttgtc actggactgc tgcttgaaaa ggacctgccg
     4921 gttgttggtc gcctggcggc acttgctgcg acgtacccga tcgaattcca tggcacgcag
     4981 gaacatcgct atacccaagg cgaatttgtc gaccggttcg atgccgcctt cccggattac
     5041 gagggatcgg taacggttga cgagttgggt cacttcaagg ccgccgtcgg ttcctgaaaa
     5101 gccaccaaga tcagtgacgg tgccagttgt agcttcgtcc ggacgttcta catcactcag
     5161 gtgcagttcc acctgacaag gagaaacacg gaaaccatgg ttaccacctc gaacactcgt
     5221 gattttgata ttaaggatcc ttcacaggca gccgcggggc atcgacgcat tgagtgggct
     5281 gcccgtgaga tgccggttct cgcgaacatt cgcaagcagt tcgagagaga caagccgttc
     5341 gaaggcattc gtgtcgtttg ctgcgcacac gtaacgaccg agaccgccaa tctggtgctt
     5401 accctgaagg cgggcggtgc cgatgctgtt gtctgtgcga gtaatccact ctcgactcag
     5461 gacgacgtgg cagcggcgct ggttgatgaa ggggtgcgcg tctacgccat caagggcgag
     5521 gacgacgaaa cctacatgcg ccacatccac tccgcgattg ctcacggtcc tcatgtcctg
     5581 atcgacgacg gcgccgatgt tgtgaccacc attcataccc agtatccgga actcatcccg
     5641 gacatcctcg gtgctaccga ggagaccacc acgggcgtca ttcgtgagcg cgccatggag
     5701 gccgacggcg tcctgaagtt cccgatcgtc gcggtgaacg atgccgatac gaagcacttc
     5761 ttcgacaatc gctacggcac cggtcaaagc acgctcgacg gcatcatccg tgccacgaac
     5821 atgttgattg ctggcaagtc tgtggtggtc gcagggtacg gctggtgcgg caagggcatc
     5881 gcgatgcggg cccgcgggtt tggcgccaaa gtgaccgtga ccgagatcga tccgatccgt
     5941 gcgctcgagg cggcgatgga tggctttgag gtcacgacga tggagaacgc cgcgaagaac
     6001 gccgacctca tcatcaccgc gaccggcaac atcaacgttg tcgatcgcca gcacttcgag
     6061 gtgatgaagg acggcgcgat gatctgcaat gcaggtcact tcaactcgga gatgaacctc
     6121 aaggcgcttg cagagatgtc tggtgagggc cgaaagactc tccggccgtt cgtcgaggaa
     6181 tacaccattg gcgacaaagg ggtcatcgtg ctgggagagg gccggttgat caacctcgct
     6241 gcagcagagg ggcatcccgc gagcgtgatg gacatgagct tcgccaacca ggcgctcggt
     6301 gcagagtaca tcgtccgtga gggcaagaac ctggagaaca aggtgtatcg cgttccggaa
     6361 gagatcgacc gcgagatcgc tgcgctgaag ctgaaggcga tgggcgtcac gatcgacgag
     6421 cttaccgccc agcaggcgaa gtaccttgtt tcgtgggcaa ctgaagacta gagcacatca
     6481 cccggccggc tactgtggac ggtggccggt cgggtttgat gctgttcctt tcgggagcgt
     6541 ctgaaagcgc cccatgtagg agccaggctg tggccaacac attcgacact tccaagaacc
     6601 tcctgttcac ctccgagtcc gtgaccgaag gtcatccgga caaggtctgc gaccagattt
     6661 ccgatgctgt acttgatgcc gtgctggctg aggatccgga cgcacgcgtc gcgtgtgaga
     6721 cgtcagtaac cacgggcctc gtgctggtat tcggcgagat tacgacgagc gcatatgttg
     6781 atatccaatc tcttgttcgc aacgtcgtct ccaatatcgg atatacgaac tcaaatcatg
     6841 gcttcgacgc aagccagtgc ggcgtgatcg tggcgatcaa agagcagagc ccggacatcg
     6901 ctggtggcgt cgacaatgcc ctggaacagc gacagcagat ggctgacgac gagtacgaca
     6961 ccatcggcgc cggcgaccag ggcatgatga ttggctacgc tgtcaacgag acgcctgaac
     7021 tcatgccatt gccgatatcg ctggcccaca gggttgcccg tcgccttgcc gaggcgcgca
     7081 aaagcggaga gatcccatac cttcttcctg acggcaagag ccaggtaacg gttgagtatt
     7141 ccgatggcaa gccggttcgg gtcgacactg ttctggtatc aacccagcat ctgcccgagg
     7201 cgaccaatga acaaatccgg aaagatgttg aggagaaggt tatccgggcc gttgtgccag
     7261 ccgagttgct ggacgatcag acgaagatcg tggtgaaccc cagcggtcgc tttgttatcg
     7321 gcgggcccat gtccgacgcc ggtctcaccg gacgcaagat catcgttgac acctacggtg
     7381 ggatggctcg ccatggcggc ggggcattct cgggcaagga cgcgacgaag gttgaccggt
     7441 ctgctgcgta tgccgctcgc tatgtcgcca agaacgtggt ggcggcggga ttggccgacc
     7501 gcttcgaagt tcaactctca tatgcgatcg gcgttgccca tccggtctcc ttcatgtgtg
     7561 agacgtttgg taccggcaag gtaagcgatg aggtgatcga gcgggtggtc cgggagaatt
     7621 tcgatctccg tcccgccgct atcatcgatc accttgacct gcagcgaccg atctaccggc
     7681 agactgcggc atacggccac ttcggaaggg acgatctcga tctcccctgg gagcggaccg
     7741 acaaggccga gacgctggca aaggcggccg gtatggttca gacagtctag gtcgtctggg
     7801 ctgatggcgt ttcgtggcgc caggatagac catccggtat tcgagactac cacctgggct
     7861 gtgaaagagt gggagagggt ggcgcgcgac accctctccc actctttcgt cgttcgcgcc
     7921 cctgaagtgc gcggcctggc ctaacggtag ttcccggaaa ccgccgtctt acggatcagt
     7981 caaaactcat cattgatagc acccggtcgc atgtgaaatt ccgtcattca cctgccccga
     8041 ctgttgctgt tttgagtgat ccaggcatat acggctatca tgggacagat gctgcaacgc
     8101 ccttgtcgtt caaagccggg tccaagaaga acggaactgc tgttcacatg aaatttgtca
     8161 tcgccgtcgt ccaggactat gactgtgaca agctgctgcg ggcgatcgcc aaagaaaacc
     8221 tgggagcgac gaaaatctcg agtacaggag gattcctgag gatggggaat gcgactgtcc
     8281 tgatgggtgt ggagaacagg cgcgtgccgg aagtaatcga actgatcagg aaacattgcc
     8341 agtcccgagt agacgtgcag ctcgacgcca cctctccgga gtacgaagag tggttcccag
     8401 caggcgttca tgaagtgaca gttggtgggg cagtggtgtt cacgctctct gtcgaccgta
     8461 tggtgcgcat catgcccgat gcgggtggat ttgcatcatg actcaagacc gatttcgctg
     8521 gcttcgccgg tactggcaag ggctactcat cgcagcagcc gtttcttacg gacaactgca
     8581 tgacacaggt ggtaccgatg tgagggcagc ccagcggcac ccctatgtct ggatgctccc
     8641 cgacgggcca gcgcagccca gtccactgga aaccgttgga caatacatat tgacgatgcc
     8701 tttcgacccc gaagccgagc gagtctggaa ttgacccccg gtttacgtct tcgcgaatag
     8761 ccaacgctac tcagcgagca cgctcgcggt ttgcttgtcg tagcgaacgt cgtccttgag
     8821 catctttccg agcatgagct gcattgccat gacgtggctg catgtaccca gcgattgagt
     8881 tcggaaagtg ttgcaggtgc aatcccaccc atccggtgtg agcttgacct ggtaggcatt
     8941 gttatcaccc tggaccgtcg cgtcgaagaa attgaatttg acgcggtctg tctcctgggc
     9001 gtaacgatgt gccttctcga tcttcccgat catgctggag ttcattgccg ataccgctcc
     9061 ttctctttta tgcgccgtgt gacgcattat ccgcacgcat ctgggacctc accgaacggg
     9121 ggcaagctgc ggagacaggg cggccttgcc cgggtcaaca aaaaacaccg ctcggggaca
     9181 tccccctgcg gtgttgcagc ttcgcgttgc acatccatct gcgatgagat gatccatctc
     9241 tgtgtccctc aagggtcgag caacaccccg acgtgccgct cacggatcat gttgtagtca
     9301 gagtatacca acatatccaa gaagggaacc cccttgtgac ggcaactacg aaatagacta
     9361 gcccgcgtac gaacgcatga aagcgcgcca ttcgactagg atggcgcgct cgtggatcaa
     9421 catctcacca aaggtctact gggcgtcttc ttcgtcgtcc atatcggaga cacccgcgtt
     9481 gccgacatag gcacttgcct cctcgtcgac ctcgtcaggc tcctcatctt ccgcttcgtc
     9541 ctcgacgtcc tcatcttcaa caccgaagtc gaagtcgccc tcttcacgaa gggagagctg
     9601 ctcgcgctca cgcataagat cctgagtggc gcggatctgg cgggggaagg tcatcaagcc
     9661 ctcattatcc tggtgacgga aggtctcccg cagaatgata cgaaggctct gagctgacat
     9721 gcccaacgcg tacgcctcga attcgttgcc gctccccatg agcttgatca cgcgggacgc
     9781 aacacgcatt tcgatctcgc ccagctgcac gccgtcatcg ctcaacacga gaaggcgctc
     9841 cccttcgacc ttgagctgca gcttatcgcc aggggaaacc tcagcaagct gccccggctc
     9901 tgccgggttt gcaagctcat ccacccacgt tttgccaatc tcctccacaa aaactgtcga
     9961 ctttgggatg gtggaatcgg ccgtcatctt gccttccggc tggcctgtgg ggcggttgtg
    10021 caacgtctcc aatcgttgaa gattgcggcg ggcgatcatg ttggcaggat ccgccttgag
    10081 ggcttcgctg tacgcatccc gggcagcgct aagctgacgc agctcgatga gtgctcgtcc
    10141 ctttcggttg agggcttccg cttcgcgcgg aaaccgctca agaatctgat cattgatctt
    10201 gagggcgtct tcccagcggc tctcaagtga tgcctccaga gcttgctggt tgagttgttt
    10261 cttggttatg ggcttttgct tcgagtttgc caagggtaat gatcctttca tacggtgtct
    10321 tttggcgagg tattggcgtc aggtggtcgc agatgctcgt gcttgcgggc ggtcgtcctg
    10381 actctggcgg ccagaattga cgccgggcgt atatcaacgc tatctcaatg aggttccccg
    10441 gacggctctc caaggaggaa ccacgggctg gttttggtga tccgcacatc caccatcttc
    10501 ccgagcacat cccggtcgtc gtcgaagtgc accagcgtat tcccacgggt tcggccagtc
    10561 cacctgccct tggacttgcc ttccacaagc acctcgtagg tctcgccaag gcgattgcca
    10621 ttgatgccgg tacacagttg ttcctgcagc ttctcaatcg cctgatggcg gcgaaatttc
    10681 tcctcatgcg ggatgtcgtc ttcccatcgc gaggagagcg ttcccggtct tggtgagtac
    10741 attgcgaggt ggaccttgtc acacttcgtt tgctcgagca tgtcgtaggt ttggaggaac
    10801 tgctcgtcgg tctcccccgg aaatccgacg atgatatctg tcgccactgt tacaccaggg
    10861 acttgctccc gggtgtaggc tatacgctcc atccagagat cgcgcgtgta ggttcgccgc
    10921 atgcgccgca atacttcatt gtcccctgcc tgaacgggaa ggttcatgtg ctcacacgct
    10981 gtgtccagat cgcggatagc gtggatgacg tcgtctccca tgtacttcgg gtgggatgtg
    11041 aggaagcgaa gccgatctag cccattgatg ttgttgaccg cctcaagcaa gtgcgcgagg
    11101 gtcgtctttt cgtggagatc gtgcccatat gcattcacgg tctggccgag cagggtgacc
    11161 tcacggacac cgttcgagac gaggcgctca acttccagaa ttatgtcgct gaatgggcga
    11221 ctggtctcct tgccccggcg atagggaacg atgcagtagg agcaaacgaa gttgcatccg
    11281 taaatgatcg gcacgaatgc ggagacgccg gccttgcccg tggcaagttc aggttggtag
    11341 tagtggggca actgagcaag atcatcctca aggtcgttaa gttccggcac gacctgaaca
    11401 agcttgtcga aatccgaggg atcgaagaag agatcgacat gcgggaatgt ctgcttcagc
    11461 ttctcctgct gacgggtgac catgcaaccg gtaaggacca gcggcatctc gggctttgcc
    11521 ttcttgaggc gggcgagaga gtgcaacttg ccagctacct tgtcctccgc ggcctggcgg
    11581 acgacgcagg aattaagcac gacgatgtcg gcttcttctt cgttgggcgc cctctcgtag
    11641 ccaacttcct gaagcatggc cgccaccttc tggctgtcag cctcattcat ctggcaaccc
    11701 atggtccaaa tgaaaaagcg cttgccgttg gtgggaaggc gccgaggtgc cacagcggcg
    11761 ggagccgagg actccggaac cagttgaatg atcggtttgg tggacaaggg aatctcctgt
    11821 agcatggtgc acactgcagc gaccctcgag atgtccccac aagggtcctt cgccgcaaca
    11881 gccaagtata gggccaaatg ggcgtacccg ttgccagcga aacgaggccg ggactgaaag
    11941 agttggagaa ggagaaaccc gtgaccgtcg ggcaccgtcc attgtagact gacagtgaag
    12001 actccaggtt ggtgaggcat ctctttaaac ctgctgatcg agcccgttga tggagtccat
    12061 ccctctgagg agcagatccg tgaagatcct tacccgtcca gttttcggtc ggcgctacaa
    12121 gctatgtcaa acgctttcgg caggggtcgt tgccctgtct ctgctcgcag gcggagcagc
    12181 ttattcggtc gctgcctcgc cgctccagcc ggcgacgatg gtatcggtgt atgcgcagaa
    12241 cgttgactgg atgtcgtcgg aggcggcggc atcactggac attgggatcg acgttttggt
    12301 gccgagttgg ataccggagc cgttttccgg agtaagcccg agcatcacgg cgagcgacgg
    12361 gtactatcag ctctactgga tggttcctgg cgggtcgccc acgtttttat acattgaggg
    12421 tgtggtggga ggagcacttc cggccggctc accggcggat ctgaacaagc agctgaacat
    12481 taatgccacc gtgcagggat gggatgcgat ccaggacacc ggtattcctg ccggtggcag
    12541 cacgccgatc tatgaccagg tgtggtggat cgccaatggc gtgctgtata ccgtcagcag
    12601 caacaacatg acgggctctg attcgctctc tcttgccaat tcgctggtcg ttctcgaggc
    12661 gcctgagctg ccagcaccca ctgaagcacc gtacgagcct ccgacggagc ctccatatgt
    12721 cccccagact gagtctccat acattccgcc ggctgagtca gtccccgtag cagaagtgcc
    12781 ggctcctagt ggccaggttg tgacggagga agtttcgaca gaacaggtct cgagtgaaga
    12841 actctcgaac gagcaggtgc aatccgacgg gaacactaca gacccagctt ccactgacgc
    12901 tgaaaccgta tccgaaccga acgccttaac tgccccgact gatgaacctg taacggacac
    12961 cacagtcaat gtagggggca cgagcgaaac gttggatgca tcaggtggta ccacgacggc
    13021 ccaaacctct gcgctgcagg gcccgtggtc gccggggcgg tatgacagcg cggtgccgtc
    13081 cgacggcacg aacggtccgc tccctccgct gatcggggga gatggcaccg gtggtcttta
    13141 cgacaccgcg ttgccgagca tccggattcg cccgtagtct gctcctcttg aggaactaaa
    13201 ggacgttcat attgcggggg agttcgaccg taaatcgggc tccccctgtt gcagcctcgt
    13261 cgactgtgat ggtgccattg tgcgcgagta ccagctcccg cacgatatag agccccaggc
    13321 cgagaccggg aacagcgctc tcgttagcag accgtgcccg atagaaagcc gtgaagagcg
    13381 attcgcgctc acgctgtgga atgcctgaac cctcgtcgct taccgagatc tggatatcac
    13441 cgtcaggctg gagagccagg ccaacggtga ccgtgcctcc gtcggtgtac ttaatcgcat
    13501 tgctgaccag gttatcgacg atctgatgga cccggtcacc gtccatccgg gcggtgagtg
    13561 aggtaaggtc ggtgtcgagg atgacattgt gcgtcgattg ggtcgctccc tttgactcca
    13621 caagtgcacg gcacagctcc acaacatcca cgtcttcccg cagtaccctc aagcgcccgg
    13681 cgcgcatccg ggagacatcc agcagatcat ccacgagtcg ggtgagccga tcggtgtgct
    13741 gctgaagggt gttcagccat gcgacatgtt gttcgtgccc gccctcccta tcgagctctc
    13801 ttgccataat ctgcgcaaag ccacggatag gggtgagggg agagcgcaac tcgtgggaga
    13861 caacggagat gaactcgtct cgcaggcgtt ccgcctcctt gagtggggcc acatcttgga
    13921 agaccccgac gacggcatcg atatctcctt cgtcggtttt caacggggcc gcgctggcaa
    13981 ccacgggaag catggtgccg cccggtgtgc ggacgaccaa ctccacgccc gtaacagtta
    14041 tcccttccgt cattgcctgg accatcggca taacgggaac ggcgtcgacc ggttcgcttg
    14101 tcacagccag ctgatccacg cggcgggaga ccagctgtga ggtatgtgcc tggagaccaa
    14161 acatccgctg cgcggcagcg ttgaacgtca cgattaatcc atgtggagga cgggccacca
    14221 tgacggcttc cggcatcgtc tgaatgacgg actgcaggcg ccggtgattg tcctggaccc
    14281 ggtcaagcag ctcttcctga gcgtcggcaa ggcgagcgga ttccatgaag gcgacggtac
    14341 gatgggcgag tgcctgcaaa cgggcaatgg cagtctggga gaaacggtag gggacgtaga
    14401 gcccgaccca cgccacaccg gcgagcgcat cggtgggacc aaaaatcggc acaccaacta
    14461 ctgcacgaac gtgaggattt gtgtttagta cgtcttcgaa ctcgggatca ctttcgatat
    14521 ccgcaatggc aatagggcgg cgctcacttg ccacacggcc cgcgaagcca tagtcgatgg
    14581 ggatcgtgcg agttgggtcg tcggcgggag atagactgct gtcggaggtg tcgccgtagg
    14641 tagccgcctg gaccaaggtg gcattgggag cgtctacaag gtagagcgcg ccggcggagg
    14701 cgcggttgac gcgcaccatc gctgtgagga tcgactgcag cacgctcacg aacgcttcgc
    14761 tgtcgccggc aggacgactt gccgcggcaa tctccacggc gttgaaagcg gtatttgctg
    14821 ctcgcatcgc cgaggcttcc agctttgcgc ttgcccggtc atatttcaga tgctcgaata
    14881 acgagacgaa gagcgcggag ccaagtccaa agaccaggag tacccagacc tggttcctga
    14941 gatcccaaaa atcaaagcgt ccgcggatgc ccagaaagaa gagatcgagc accagcagac
    15001 tggtcagcgc cgaggtcaac cccccgaccc aatccgcgac gtaagtggca agcgcgacag
    15061 agaggagaaa gaagacgatg ggtatctcag gaccgagatg gggcgtgatc gacgcaagca
    15121 gaagtgcggg gacaatgacg atggtgaagg caaagacgag ccgccaaggc tcgatacgcg
    15181 ccgcacgcat cgaagtcaca tgatcgctgc cgatgctggc ccagtgacga gtattgctgc
    15241 ccagttccgc catggcatgg cctatgcccg ccagtgttgc ggacgcacgg tcccaggtcg
    15301 tcggagacat ccgggctcgg gcccagttct tgaatcgctc gctactcacg ttgtgcccct
    15361 gtacctacga tacgtcccgt aaaattgtac cgggaacacc aacgcgttgt ggctaaccgg
    15421 aacatcggcg atgcgccctc gcgggtccta agcgcgcccg gtgtaaaatc cctgaaatgg
    15481 atcgccaggt gcttcctcct atcggacggg caatggcaca taactgagca ggagcatgtt
    15541 gcccgtgagc aaccaactga agcagcagac agacgggctc gtgatcgatg agaacgagca
    15601 ggtctttgac gtgaacatca tcggggcggg gcccaccggt ctcttcgcgg cgttttatgc
    15661 tggcctacgg ggcatgagcg tgcagatcat cgactccctt gaggaggctg gtgggcaatg
    15721 cgccgcaatg tatcccgaaa agtacatcta cgacgtcatc ggttatccga aggtgctcgc
    15781 caaggatttc gtggctgcgt gcctcgaaca ggcaatgagg tcagacccaa cgattcggct
    15841 gtcggaacag gttcaggacc tcatacagct ggatgatggc agcttcgcga tgaagacgag
    15901 caagggtgtt cggcgtagca aggcgatcat cattgccgcg ggagttggag cgtttgaacc
    15961 aacacggctc acggcgagtg gtgtggtgga actggaaggt aatggcgttc attactttgc
    16021 gaagcgcgtg gatgacttcc gggacaagaa ggtggtgatt gttggaggag gcgattcagc
    16081 ggttgattgg gcggccacgc tcgagccgat cgccgagagt gtcaacctca tccatcgatc
    16141 gaagttccgg gcacatgaag cttcagtgca ggaactcgag gaatccactg tcgtgcttca
    16201 ttaccccggg tacgaggtca ccacagttcg tgcgggtgat gacgggcgca tttcaggtct
    16261 tacgttcacc catcaggacg gcacgtcgac tgacctcgaa tgcgacgagc tgatttgtgc
    16321 gatcggcttc aaggccgatc cgggtccgtt acgctcctgg ggcttcgagc tgaagcgtaa
    16381 ccaaatcgct gttgatatcc agacgatgca gaccagcatc ccaggcgtct tcgcggcggg
    16441 tgatatcgcg acgtacccgg cgaagttcaa gttgatcgcg accggagctg cagaggcggt
    16501 tacggccgta aatcacgcgc tcgtccatat ccgtcccgat gcccggctcg atggtgggca
    16561 ttccacgacg atcatggaga agcgggcaaa agtagtggaa gccgcggtat aggcataagc
    16621 agcatgtcgc atgcagttgt cgcccggaaa ggcgataacg gtggtggatg ggcacaagtc
    16681 ggaaacaaat gagaaagggg gacgacatgc gtcgtccccc gtcttgtgca actttcggtc
    16741 cgatttgtag ctacccggct ccacccagtc ccattcgctc gcggacgagc cgcatcttgg
    16801 cagaggcaat cgtgcgggct tcctcagcac cttcggcaag gatagcgtcg acgatctccg
    16861 gcgattcctc gagctcccgg aggcgccgct ggatcggcgc cagcgcgtct acgaccacct
    16921 ctccgagctc ctgcttaaaa gggccatacc ctttgccaat aaacttttcg gaaacgcctt
    16981 caagggaatt atccgagagg atcgcgtaga tcgacatcag attcgtgagg gctggtttgt
    17041 cacgcccggc gacgatctcg gagccggaat cggttaccgc tcgacgaatc tttttccgga
    17101 taacgtctgg ttcatcgttg agcgcgatgt agctgttctg gttcggagac gacttgctca
    17161 tctttttcgt cgggtcgtcg agcgccatga tccgggcacc ggttgtcttg atgtccggct
    17221 cgggcaagac aaaggtctcg ccatagcgct ggttgaagcg gtatgcgacg tcacgggtca
    17281 gctcgatatg ctgcttctgg tcctcaccga cgggaacctt attcgcgtca tagagaatga
    17341 tgtcagccac ctgcaggacg gggtagacga agagcgcggc acttacgcct tcctcgtgtt
    17401 cgcccgcctt gtccttgaac tgcgtcatcc ggcgcaactc accgaactgg gtcacagatc
    17461 caagcagcca gcagagctcg gcatgctcgc gcacatctga ctggacgaag agaactgctt
    17521 tacccgggtc aacaccggct gccactatcg tattcgcgag gttacgaatg ttgctctgca
    17581 tgctctcgcg tgtcgtggcg agggaaaggg cgtggaggtc gacaatgctg aaaacgctgt
    17641 cgtattcgtc ctgttgcagg acccagttcc tgatcgctcc gagataattc cccaggtgaa
    17701 gattgccaga gggctgtatt ccggaaaagg cgcggggccc gcttctcctg gtctccgtgc
    17761 tggatgtctt gtctaccgtg gctgccatgt gcgcgtgcgt gtccttttgt ttccccatgc
    17821 cgaatggcat gcatgtattc gacctgcgac agtataccgg gcaccgtgga gcatatgatt
    17881 cctgcaaaca gggttgattg gaagcggccg caactggagc gcgaatgatc gagaccatgt
    17941 tcctgcaccg tcgccatgcc aggcgttacc ggcaaatcgt gcgtgtgctg ctctcgcatg
    18001 gcctgggagg cctcatcgcg cccttcgatc cgcgaggccg ccatgatgca gagcaggaag
    18061 atgtccagcc ggccgggcgc gtgacagccc gccgcgcacg ccatcttcat cgtgctctcg
    18121 aagagctcgg gcccacgttc gtgaagctgg gtcagattct ttccacccgt gccgatcttt
    18181 tgcctccggt ctatatccat gagctggagc agcttcagga tgctgtggcg cctgacgacc
    18241 ccgccgccat ccggaggatc gtggagcagg agcttggtgg ttccattgag tcactttata
    18301 cctcattctc ccttgtgcct ctcgctgccg cgtccatcgg ggaagttcat gcggcaacgc
    18361 tgcacgatgg ttccgaggtg gtggtcaagg tgcagcggtc gggcgtagct gcgctgatcc
    18421 agcaggactt gtccatcctc gctgatgtag cgaggatcgc ggaacagcgc tcagccaaac
    18481 tgcgacagca tgatgtctcc gggcttatcc gagagttctc ctggacaatt cgcgcggaac
    18541 tcgattacca gcatgaagca cggaacctcg agcgcttcca ccgtgccttt gcttccaatc
    18601 cggcaatccg tattcccgca gtggtgagag acctgacgac atcgagggtg ctcaccatgg
    18661 agcggatcga tggcatccca atcgatgatg ttcagtccct gcgcggggcg ggccacgaca
    18721 cgatcaagtt gattgaatcg gtcatccaaa tgctcgccga gggcattctc gatatcggac
    18781 tcttccatgc cgatccccat ccaggaaatt ttgtggtgga tcaggatggc gctctggtcg
    18841 tctacgactt tgggatggtc gggtccatcg acgagcgatt gcgggagcgg ctcctgttgc
    18901 tgacgcttgc ctgcgccgag agagattcct cccgaattgt tgatgagatc acccagcttg
    18961 gtgccgtgca gcccggctgg gaccgacagg cgatggagcg ggatatctcc tatctcgtag
    19021 gccaatatgt cggtgcaccg ctggagtccg tgccacttcc gatgatcgtt ggagatgtga
    19081 tggcaatgat gcgtcgacat cggcttcgtt tacccagtga gctggccttg cttgccaaga
    19141 cggccaccat ggcggagtcg cttgcccgca cgctcgaccc gcagctcaac gtgatcgagc
    19201 tggtggatcc tacgatccgc cacgcgatga agcgattttc ctcattggga ttctggaagg
    19261 accgattgcg ggcgcggccg ttggagatcg cgctcctggg tgcatcgttg ccggggcaca
    19321 tccaacgcat cctctccagg atcgaccgca atgacctgac gtttcacgtc cattacgagg
    19381 agctacccga gactatccag ggcctgaata gtatggtcaa tcgcctcgca ctggccattc
    19441 tttcggctgc gggaatgatc ggtttcgcga tgctctatct ggcgatgcaa ccgacattcg
    19501 ggagctggca tggatggctt ttcggaacgg tctttctcgc catcgcggta atgaccctga
    19561 ccgtgcttgt ccggatatgg agaagctgag gggaggcgat tctcacaaga aaagccccat
    19621 ctgttcgctc atccacgcga gatcggaatg aagctactcg cattcgcgag cgcgggcccg
    19681 tgcgctctac gcttcttcag gtccgcagtc gtcgtcgctc agccagtccc gcccgaactc
    19741 gcgcatgctg tcgataaccg ggaggagggc atagcccttg gatgtcaagg tgtactcgac
    19801 gcgcggcggc acttcggcga aacagcgccg gtcaacaacc tcgcaatcct cgagcttttt
    19861 gagacgctcg gaaagtgttt tcgggcttat accccggagg gatcgttcca gctcgctaaa
    19921 ccgcttctgc ccgtgagcaa ggtcgcgcac aataagcggg gtccacttgt ttcctatgat
    19981 ctcagccgtc cgggcaaccg gacagccgac atcctctgtt ctagatgttg tcgccatggc
    20041 gcgtccctgt tccggtgaaa tccttcaata ctttccatat gatagcacga gcagtgtgct
    20101 ttctgatctc ctcaggtcca tcgctggcca tctcgagtta ccagaggggt ggacgatcac
    20161 gctgatgttg gcgatcaaga ccggaggaat tgcggctgcg aagagttgtt tacgccgcga
    20221 ggagttacga tcaacgccac gtggtcgccc ctaggtgatt ttgcgctcgt cattgcccgc
    20281 tctcccggtc gagtaactcg cgtttccgct cctgtcccca tcgatagcca gcgagatcgc
    20341 cgttcttgcg gacgcgtcga tggcagggaa tagcgaccgc gaccgggttg gtcgcacagg
    20401 cgcgggccac agccctgacc gacgatggcg cgtcgatcag tgctgccagt tccgagtatg
    20461 tgggtctcgt accagttgga accttccgta aggcgttcca gacgcgatgc tgaaacgcgg
    20521 tcccgcggag attgagcgga agtcgaaggc ttccggcggg tcgttcacca tacgaattac
    20581 ctccgcaacg gtttcctcga aggccggatc agttccgaca atcaccgccc tgtcaaactg
    20641 ttcttgcacc tgcgcgaaca aagtctctgg atcctcccca aacccgatcg agcaaatccc
    20701 tttgtcggtg gctgcaatcg cgaaggccga gggacgtgtc ggcaattgca aaacgaatac
    20761 gctcgcccgg gccactatca cgatacgccg atggagttat gccaatagtc atatcggttg
    20821 acgcgtaaaa acggcccgag gattggtatc cggtttcgta gacggcctgc gcgacagatg
    20881 gcgatgtcgg tagctcttca cgcactcgtg cggcgcggtg gcttcgggca tactgcccgg
    20941 gcgtgaggcc ggtgatcgac ttgaaggtgc gatggaaatg gtatgggccc atgcccgcgc
    21001 tcgacgcaag gtgcgcgagt gttggcacgg tgtcagcggc cgcaatcgat tcacaggctt
    21061 cggcaatcac ctcgagatgg cgctgacggg tagaagttcc agaaggattg caccgtttgc
    21121 aggggcggta tccctcctgg atggcgcgtg ccggggtgtc gtagaactcg gcattttcac
    21181 gacgcggggt ctttgcagga cagccgggac ggcaaaatat gtacgtggtc cgcaccgcga
    21241 agacaaactg cccctcagcg gatacgtccc tcttggttac ggctgaccac cgggtattgg
    21301 cgctgccacg ttgagcgtcg ccttgtgcca cgttcatcgg tgtcctccct aaccctgttg
    21361 atgcttgatg gtccaacagc catctgccac catagaggtg gatgcaaaca tcggggacaa
    21421 tctgtctctt gctttcaaat tgtgaggaat atctaggatc aggacacgaa gtgcacgtat
    21481 actcgacgct ggctccgtta ccctaggcct gctgtcgtac gagatagacg aagtggacga
    21541 atgatccgac ttattgccta tgcgctctcc gcggtgattg ccgcattggc caccgcgacc
    21601 atctccgagc gattgctgac atttgatacc ccccagagcg tgattatttt cggcctggtg
    21661 atgggcgtca ttgacgcatt catcaagccg atcgtgcgcc ttctgtcttt tccgatcaca
    21721 tgtcttacct ttggtctctt ctccctcgtg atcaacgcgg cgttattcgg cctcggatcc
    21781 tttctggttc ccggtattga agtgagctgg gtaggtgcag ttctgggatc gattcttgta
    21841 agcatcgctt caggcgtgat cttctcggtg cttgatgagt aggcgaagcc aggcagcagg
    21901 accgacaacg cgggagcgga atttcgatgc tccccttacc atcggcgtga tctccgacac
    21961 gcacatctat ccccatagtt cccgcgagct ccaccctgcc ctgatccgat tcttccgacg
    22021 cgcgaatgtc gggttgatgg tccatctcgg cgatgtcaac tcccggcccg tgctggacga
    22081 aattgccgac attgctcctc tcatcgccgt tcacggaaat aatgacgacg aggaactgca
    22141 ggtggtgttg cccagaacaa cccgcttcag tgttggcagg ttcacgttcg gcgttattca
    22201 cgggcatggt ggtcgctcgg cccgggatga agcaatcaag cgatgggttg gcaaggtcga
    22261 ttgcgtcctc ttcgggcaca gtcacaagcc gcttctggag aagatcaacg agacagttct
    22321 gttcaaccct gggtcggccg tcgaacgcag atggcatccg catttcggcg tggggctcat
    22381 taccatcgag gaggatcggt ttactcccga cttggttgtt ttcacacacc cggaacacct
    22441 ggataatgtg caagttggga cggatgaaac cggatcgcgc atgtgatgta gttcgtccgt
    22501 tcgtatgttt gcatgagagg atagtgccac gtggatccca gactcgatca aggccgatgg
    22561 ttgcgcattc ccggaccaac gcctttgcac ccggatgtcg ttcaggccct gacgcacgag
    22621 atggtgccgc atcgcgggcc agaggtacag gagacgatgg cgcgcatcca gccgatcgcg
    22681 aagagggtgc atggtaccga gaaggggctc gtgctgctct gggcaggaac cgggaccacc
    22741 ggtttcgagg cttcgattgt gaaccttctg gcaccgggtg accacgtcgt agtgacggtg
    22801 gccggtgatt tcggtggccg atacgcgcgt gtggccgagg cgctccagct tcacgttcac
    22861 cgtctggaag ttgagtgggg cgaagcggtc acccccgaga tgctgaaggc cgcgatcgcc
    22921 gcgcacgggg atgtcaaggc agtattgatc acgcataacg agacatcgac aggcgtcacc
    22981 cagccattgg atgacctgtc atctgttgct cgcgatgcgg gagcactggt gctcgttgat
    23041 gccgtcagct cagcggcagc gcttccgatc aacatggatg agaacggttt ggattgggtg
    23101 ctatctggtg cgcagaaggc gtggatgtgt cctccgggat tgatgatcag cgctctcagc
    23161 gagcgcgccc ttgaagtggc caggcaccaa aagggatatc cccgggcgac ttgggacgca
    23221 atcgccatgg cagaggcatc ggcccagaac taccacccga cgacaggccc gctcacagcc
    23281 ctgttcgcac tggacgccgc acttcaggcc gtacagaccg aaggcattga ccaactcttc
    23341 gagcgccaca agagactcgg aacccacctc cgggagggca tggagcggat cgggatcgag
    23401 atccttgcag atccacggta tgtctccgac tcagtcaccg ccttcattcc gccgtcgggg
    23461 atgagcgcgg cgacgttccg cgacaaggta cgcgatgcgt cgggtgttga gatcgcggta
    23521 ggacaaggta aacttgctga tagcgtgaac cggattggtc atatgggctg ggttgcacag
    23581 ccggaacttg atgcgacatt ggaggcactt gaggctgcaa agtaagcgct ttgcatttga
    23641 gaacctattt tggcgagcag cgtttatcgt tgtcctgaat gggattttcc ggtatgattg
    23701 tggctagcct ccccggaaag ggcgattccg ccctgaaata atccggtgtg aggattgttg
    23761 cgtgactttt ggtttttaag gatggtctat ggacggtacg gacgttcgtg agcaagaagc
    23821 acaagtgaac ggtgatgagc aggagcagac aatggatgct tctgcacaaa ccaccgctaa
    23881 cggggtcgaa ctcgacgagg agctcgatgg tgcctcgctc atggagcaat tcctcagtga
    23941 tcccagccat gactacaaga ccctgaaata cggggacgtc ttggaaggca tcatcatgca
    24001 cgtcgatcgg gacgagctgc ttgtggacat cggttccaag gcagaaggca tcgttccgtc
    24061 gaaggagtac tcgagccttt ccactgagga gaaggacaag ctccaggttg gcgacaacat
    24121 tctggtcttc gtggttcagc ccgaaaaccc ggaaggtcat ccagtcgtca gcatcgatcg
    24181 tgcccgacag gagaagagct ggaggcgctt gcaggagctg cacgaggcca acgaggtcat
    24241 cgaggcggag gtcaccaatt acaacaaggg tggcctgctg gtgaatctcg acggcgttcg
    24301 tgggttcgtt ccggcatctc aagtcagcga aatccgcggt ggtgatgagg ctgcgaagca
    24361 ggcggatatg gcgcgcctga ttggcagcag tctgccgctc aaggtcattg agatcaaccg
    24421 acaccggaat cgcctcatcc tctcggagcg gcaggccgtt caggaacgcc gcgacgtgat
    24481 gaaggagcag ttgatccagg aattgcatga gggcgagatt cgcaagggcc gcgtctcctc
    24541 gatctgcgac ttcggtgcct tcgtggacat cggcggagcc gatggactcg ttcacctttc
    24601 cgaactgagc tggagccgcg ttcgccaccc aagcgaactg ctcagaatcg gcgaagaagt
    24661 agatgtctac gtgctgggca tcaatgccac cgagaagaaa attgcgctct cgatcaagcg
    24721 cacacaggcg gagccctgga gccgcgtcgc gaactcatac gaggtcggac agctcgtcaa
    24781 gggtacggtc acccagttgg caaactttgg tgcgttcgcc cgcatcgagg acggcatcga
    24841 gggccttatc cacgtctcgg agcttgccga cgagcggatc cagcatccga agcaggtcgt
    24901 gcaggaaggc caggacctac tcctgcgaat catccgcatc gatccgcagc gtcgccggat
    24961 gggcctcagc cttcgacgcg cgctcgatac gcccgacgct gagcttcagg cagtcttcgg
    25021 cgaggaaatt ctcgaggagc gtgacgcgct tgcccagcgc atccgcgtgt cccttgatgc
    25081 tgaaggactg acgggaacac gccaggatga gcctgctcct tccgaggttc cggccgccag
    25141 aaccgaaacg ggaaccgcag agccacttga tgtcgacgcc gcgacgccgg cgcttggcga
    25201 agcccaggta gccgcgttcg aggagcgcag ccgccgtgcc gagcaggccg cccagcagcg
    25261 tcagcagcgc cagcgtgctc gacagcagca acagcagcaa gagggcggag ccaacttcga
    25321 ggacgaaagc atgtctgcga tggcgatggc gtttgccatg gccggagccg atgctgacct
    25381 tctcgcagga gccgacgagc aagccgccgc ggcagcggcg gccacggagt ctgaagacac
    25441 cgtcgctccc gccgccgaag atggtggcga tgcgaccgaa gtcacggaga aggacgtact
    25501 ccaggacacg gttggtgatg aggaatcaag cgaggggtaa tcggacatat tcgtgtcaac
    25561 cgggtgactt ctggttgcgg aattgcatag aatagtcaat accggcaggt gtcattgagc
    25621 acatgccggt attgtccatc cctgaccctg tgacaggagg gccaatcggg tataggatta
    25681 gaaacacaag ggcaagctcg aacgttgaag atgtacagcc atcgggcgct ggtcttggat
    25741 tggaagtcac gcaggtatca cagtgacgca aggtgatttc attcatcgat gctcaccata
    25801 agacgggaga gccaggatgg cggagaagtt cgacaaattc acagatcgag cccgcaaggt
    25861 cctcacgctc gcccaggaag aggcgcagag gttcaaccac aattacatcg ggaccgaaca
    25921 tctgcttctc gggttggtgc gtgagggtga cggagttgca gcaaaggtgc tggcgaacat
    25981 gggcgtgcaa ttgcccaagg ttcgctcggc agtggagttc atcattgggc gtggggaaac
    26041 catggtgctg gccgaaatcg ggctgacacc acgcgccaag aaggtgatcg agctggcagt
    26101 ggatgaggca cggcggctga atcaccatta catcggtacc gagcacctgc tgttgggcct
    26161 cgttcgcgag ggcgagggca tcgccgccgg tgtccttgaa agcctcgggg taaacctcga
    26221 gaaggtccgc acccaggtca tgcaggttgt cagtcagggc ggctcctcgc catcctcggc
    26281 agggccatcg ggcaagggac aggcgcagac caagacgccg tacctcgact cgctcggctt
    26341 tgatctcaca gaggcagcac gctccgataa actcgggccg ctcgttgggc gaagcaccga
    26401 gattgaccgc gtgatgcaga ttctctcacg ccgcacgaag aataatccgg cgttaatcgg
    26461 cgaaccgggc gttggaaaga cggccatcgt cgagggcctt gcccagcgaa tcgtttccgg
    26521 tgatgtcccc gatcagttgc agaacaagcg attggtcgcg ctcgacatcg gagcgcttgt
    26581 cgcgggcacg aagtatcgtg gtgagttcga agagcgcctc aagaagatcg tgaacgaggt
    26641 caaggaaacg aagagtatcc ttttcatcga tgagcttcac acgctagttg gtgccggggc
    26701 tgccgagggc gcagtggacg cggcgaacat actgaagccg gcgctttcca gaggcgagct
    26761 ccagacgatc ggcgcaacca ccctcgacga ataccgcaag catatcgagc gcgatgctgc
    26821 ccttgagcga cgcttccagc ctgtgcacgt gcaggaaccc tcgatcgagg aaacgatcca
    26881 gatcctcaaa ggcgtgcgac agttgtacga ggtgcaccac aagctcaaga tctctgatga
    26941 ggctcttgat gcagcggcca acctcgcggc acggtatgtc cctgaccgtt tcatgccgga
    27001 caaggcgatt gacctgatcg acgaagcatc ctcgcgagtg cggatgcaac gatcggcggc
    27061 gcctcctacc ttgaaggatg caatgaccgg actcgggtcg ctgcagaacg agcttgagga
    27121 cgcagtgaac agccgcgagt tcgagctggc tgcagagctg cgagaccgcg agcgaaagct
    27181 cagggatcgc atcgagacga tggaacgcga ccgccgcgag acccaggctg ccgaagaggt
    27241 atatgtcaca gaggaggatg tcgcaggcgt cgtctcgatg tggaccggga tcccgctggt
    27301 tcggatcgcg ggcgaggaat ccgagcgact gctcaagatg gaagaggtcc tgcacgagaa
    27361 aatcgtggga cagggcgaag cgatcacgac catctccaag gcagtgcgtc gagcacgagc
    27421 ggggatcaag gatccgcggc gacccattgg ctcgttcatc ttccttgggc ccaccggcgt
    27481 cggcaagagc tacctggcgc ggatgctcgc agagttcatc ttcggatccg aaaccaacct
    27541 gatcaagatc gacatgtcgg agttcatgga gcgccattcc gtcgcacgcc tggttggtgc
    27601 acctccgggc tatgtcggct acgaagaggg cggtcagctt acggaggcgg tgcggcgcaa
    27661 gtcgtactct gtgatcctgc ttgacgagat cgagaaagcg caccctgaag cattcaacat
    27721 gctcctccag atcatggagg atggatcgtt ggccgatgcc aaggggcgcc gggtcgattt
    27781 ccggaacacg atcatcatca tgacgtcgaa tatcggcgcg gaagaaattt cccgggacat
    27841 gacgatgggc tttgtctccc gtcaggatga ggagggagag aagtctcgcg agcacgggct
    27901 gatgaaggat aaggtcatgc agcgtctgaa gcagacgttc cgaccagagt tcctgaaccg
    27961 gatcgatgct acgatcgtgt tccattcact gaccaagctc gagatccgcc agatcgtaga
    28021 cctggagatg gagcgggtgc gcgagcagct gaaggagcaa ggcattacgc ttcagatcac
    28081 cgacggggcc aaggatttta tcggcaagga aggctacgac cacacctacg gtgcgcggcc
    28141 gctacgtcgg gtcatccaga accggatcga ggatccgtta gccgagggtc tgttggacca
    28201 gcggttcccg agcggcacca ctgtcaaggc agatgtcaag gatgacgaga ttacgctgga
    28261 atcggtcgag acagaggcaa acgaactcat tcccgtatag gtggatgacc gatacgaggc
    28321 gaagaaacgg cgcgacggag aatcccgtcg cgccgttcct gtccagagag cacatcatgg
    28381 caaaaacacg cacaacctgg atctgccaac aatgcggaac agcaagtccg gcgtatctcg
    28441 ggcgatgtcc cgggtgcggg cagtggaata cgatggtcga gaccatcgat gaacgtagct
    28501 caccgaaggc cgctgcacgc aggcgggtat ccgggacatc agcactcaag ccgctgaacc
    28561 agatctcgcc ggaaggccag gcgcgcacgc ctgtcggcat cgccgagttc aatcgggtac
    28621 tgggtggcgg attggtccct ggctcccttg ttctcgttgg tggcgatccg ggcattggca
    28681 agtcaacact tatcttgcag gctgccggcg cactggctga accgaacgcg ccggtcgtct
    28741 acgtctccgc ggaggaatcg gcgcagcaaa tcaagttgcg agccgatcgt ctgcgggtgc
    28801 ggtccgacga catcctcgta ctctcggaga cgaacctgga cgagatcctg gcgacggcag
    28861 aggcggcgaa gcccggtttg ctggtcgtgg actctatcca gacggtgatg ctcgacgaga
    28921 tcacatctgc ggcgggaagc gtttcccagg tgcgggaatg tacgagccgg cttatgcaat
    28981 gggctaagcc gagaaacatc ccggtgttca ttattggaca cgtgaccaag gaaggtacga
    29041 ttgccggtcc gcgcgtgctc gagcatatgg tcgatgcggt cctctacctg gaaggtgagc
    29101 ggtttcacca ataccggatc ttgagagcgg tcaaaaaccg gttcggatcg actgatgagg
    29161 tcggcgtctt cgaaatgatt ggagatggcc tgcgcgaagt ccggaaccct tcagaagcgt
    29221 tccttgagga gcggtcgtcg attgctcccg gttcaactgt ggcggtgacc atggagggct
    29281 cgcgcccgat actggtggaa gtccaggcgc ttgctacgcc gagtagtttc tccatgccgc
    29341 ggaggagtgc gaacgggttg gacacgaacc ggctccagtt gcttgtggca gtcctgcaga
    29401 agcgcgtcgg actcgggctc gggacccaag acgtctacgc caacgtggtt ggaggattgc
    29461 ggatcatgga accggcggct gatctcgccg tggccgtggc cgtggcgtca agcttgagag
    29521 acaggccagt cgagccatca acggtctgca ttggcgagat tgggctctcg ggggaactcc
    29581 gaagcgtcaa tcacctggag cggcgtgttc aggaggcatc cagactcggg tttcggcgag
    29641 cgatcgttcc ggaacgatcc gcgaacctgc ctgcggtatc gaaactcggt atcgagatca
    29701 tcccggtttc aactgccgcc gaggctatcg aggcggggag tccctgacta tgagcgctca
    29761 ctgtaatcaa cgagggtgag gagctcatcg acaaggcggg cggcggcgtc cgggcgggca
    29821 atgcgccgag cgttcgcgcc catcgcgtgc cgtcgcatag ggtcggccag gagtgcgacg
    29881 atctcctcct cgaggcgctc gggtgtcgcg tcatcctgct cgatcacggt tgcaccgtcc
    29941 gcacctgcca acaggttcgc gttcttgcgc tgctcgtctc cccacgttcc cggcaacggt
    30001 acaaggatcg aaggcttgcc aagaaaggcg agttcggcga ctgttccagc cccggcgcgg
    30061 gcgatcacga gatccgtgat ggcatagagg tcggcgatct cagggcccag gaattcctga
    30121 acgacatagc gctcacgtac atgctggggc caggaatccc gcaacgagcg aaggcgggcg
    30181 gcgtcctcat ttgcaaccga gggaccggac tgatgcacga tctgggtctt ctccagcagt
    30241 cccggcaata gcttttcgac acgttggttg atggggcttg caccgcgcgc cccaccggtg
    30301 acaaagatga ccggaagatt cggcgacagg ccgaagtgct cccaggcttt catcttcgtg
    30361 ccctcagtca gggaagcccg gaccgggttg ccggtaacca ccacgtggtg gtggtatctt
    30421 cgcgcaattt tggcagtctc gtcataggat gcggcgaagc gttccgagaa gcgggccgca
    30481 atcttgtttg cgatgccgac ctgcgctgtt tgttcatgtg tgagaacagg cgccactcga
    30541 gatccgccga caacggtcgg aacactcaca ttcccgcctg tgctgaagat cacatccggt
    30601 ttgaaggcgc gtgctttgat ccatgcctga gcgatgccca ctggaatacg aaacaggtca
    30661 gttatggtct ctaatgagag atagcgccgg atcttgccgg tctggagtgg agcgaacgct
    30721 ataccatgcg agtgagcgat atcccgttcg acgcccttgt agctgccgag ccaaagaatc
    30781 tccgccggta tctctcgtga tttgagctct tcgataaccg cgatggcggg aagcacatgt
    30841 cctcccgtac cgccggcggc gataaggagt cgcagcggtt tctggttcga tgtcacaact
    30901 gtcccttggg ctgaatcaag tggtccttag gttgatgctc aacgtcattc aactgcacgc
    30961 cagtatagtg ggcgcgggag gggcccaatg gagagaacct cagataggag tgacaggttc
    31021 tactcctgat gacaggcgac ctgccccgct ggttgggcgt gctaccatcc ccgtcaggca
    31081 gtaagtcgaa gggggccgga cgatccggag gaccacgctc tcggagaacc aatgtcagac
    31141 gcaccaggac gacgttgcgc gagtccccgt cccatctttc cacttctggt cttgctcccc
    31201 atcctgatcg tcgtgctgga ccagttgctc aaggccgtca tggtccagtg gcttggacct
    31261 gaatcgaccc tgcaccggtg ggagttggcc ggaagctctc tcgctttcga gtaccttgag
    31321 aataggggag cggccttcgg gattttgcca aatcaaacgg aactgctgac ggtgctatca
    31381 accgggattg cggtcgcagg tattgctctt atgtggcggg aatccaggac gcatccgatg
    31441 atggcgatcg cgattggtat ggtcgtcggg ggagcggttg gcaatatcat cgaccgtatt
    31501 cggctcggtt acgtcgtcga ttttatcgcc gtcggagtct ggcccaaatt caacctggca
    31561 gatgcaatga taacgatggg cgttcttcta ctgctgtggt cgacggtaca ggaagaacgc
    31621 cgtgacacgc gctcaaatca gggaatcaat acgcatgaat gaacgcacac caggtaccct
    31681 gttgggggtg gactccgagg atatcgcgga aattattgag ctccggccgg acccctcaca
    31741 caggaatatg aggcttgacc gttatgttgc cggcgagttg cccgacttga gtcgcacgta
    31801 tctccagtca ctgatcgacg aaggaatgtt gctggtcgac ggtcgaaccc ggcgggctgc
    31861 cttcaagatg acccctggag aagttgtcac tgtggcgctg ccggtcccac gccagttcga
    31921 tctcgagccg caggacatcc cgcttgatgt cctttacgag gacgcagaca tcctcgttat
    31981 caacaagtct agcggcatgg tggtccatcc agcgccggga catccgaagg acacgctggt
    32041 gaatgcggtc ctgtatcacg ccccagagat ttcgatccaa ggttccaccc ggccgggaat
    32101 cgttcaccgg ctggacaagg acacctctgg cgtgatggtg cttgccaagt cggatcgggc
    32161 gcaaacctcg ctggttgaac aatggctgga acggtcggtc gtcaagcact atgcggcgct
    32221 ggtcgccggg attatcgagg aagaagaggg gacgatagac gccccgatcg ggcgcgatca
    32281 ggttaaccgg caacgtatgg gcacgagccg tgccggacgc gaagcgatca cgcattttca
    32341 cgttcaggag cgatttgatg acgcgacgct gctggatgtg cggatcgaga cgggccggac
    32401 gcaccagatc cgagtgcacc tggcgtttat cggtcacccg gtggtcggtg atgcaatcta
    32461 tggaaacaag gtctcagaga ggattgctcg tgatcatggc gccacccggc agttcctgca
    32521 tgcgtcttcg ctgacgttca agctaccggg tacagaggaa atgcgcacct tttctgcccc
    32581 acttcctcac gagttggagg agctgcttga gcggttccgc gaagggtcgg agtccgatgt
    32641 tgcagaggta tgaggagata catgaccgga tcgaacgctg gtaccgggcg gcactcgagg
    32701 tggttgagcc gaaggcagcg gtagcccggg cgatggcgtg ggacggcgaa aggctcgtcg
    32761 ttgacggcca gtctatccca gtcgatatga acgtccgcgt gtttgtggtc gcgattggca
    32821 aggccgctgt cgcgatggcg gaaggcgccg ttgagggcct gggtgaccgg attgatgacg
    32881 gcatcgtgct caccaaatat ggtcacctag gcgccggaat acgcggtttt cactgctttg
    32941 aggctggcca tccaatcccg gacgcgagcg gcgagcaagc gacgcgcagt atcatcgatg
    33001 cttttgaagg actgacatcc aacgatattg tccttgccct gatctcggga ggaggatcag
    33061 cgctcctgga gctaccacgc gaaccgctga ccctgcagga tcttcaacaa acgaccgagg
    33121 tgctcatgca tgccggagcg ggcatccacg atctcaatgc ggttcggagc gtgctcagtc
    33181 aagtgaaagg cgggggactt cgccgccata taggcgcggc tcgatgcgtc agccttctgc
    33241 tgtccgatgt attgggcaac gatccccagg ttatcgcgag tgggccgacc gtcctggtac
    33301 atcgatcttt tgcggaggca caggcggtcg tttcacgctt tggcgtggag ggtcgactgc
    33361 ccgagtcagt gcgcttggtg ttggccggag aagatcggat cgatagtact cccgacagca
    33421 gccacgatgt ctggactgtg atcgccgaca acgctgcaat ggtgaatgag ttgcagaccg
    33481 tcgtggaggc cgaggggtta tccgcgcgac ttcagtggca ggcctacgat ggcgatgcgg
    33541 caaatcttgg acgcaagatg gtggaggatg cccgcgcgtc ggacgatacg tgggatgttc
    33601 tcctcggtgg cggcgaagcc accgtcgagg ttaccggaca gggctccgga gggcgcaaca
    33661 cggaggcggt gctcgtatcg gctatggctc ttgagcaaca aggcggagat tgggtcgttg
    33721 ccagtctcgc ttcggatgga gatgacggat ccgccgatgc cgcgggggct attgctgatc
    33781 cggagaccgt tgctcgtggg cggctggccg gcgtggaacc atcgacatcg ctggccaaca
    33841 gcgacagtgc atcctacttt cggaaggccg gcggtcttgt cgtgaccggc ccatccggca
    33901 cgaatgtcaa tgatgtctat attgcggtgc ggatacgacc gactttcgat cagggtgaga
    33961 attgatggaa aagcgaccgc tcatgttgga tgtcgatacg ggggtcgacg acgcggcggc
    34021 aatagcgctg gcgattgggc tgaaatcaga accggtcgcg atctctacgg tcgccgggaa
    34081 cgtccccatc gatgcgtcga cggagaacac gctccgcgtg ctctccctgc tcgacaaggg
    34141 agatgtgccc gtctatcgag gtgcaagccg tcctctgaca gccacgtacc aggatgctgc
    34201 ccatgtacat gggagtaatg ggctgggcgg tgtggagtta ccacctgcga gggcgaccca
    34261 gtgtgactac acggggccgg aggcaatcgt tcgaatggcg gagcgatatg ccggcgaact
    34321 gaccctggtg acggtcggac cgttgaccaa tctggcaatt gcgctgtcgc tgcgtccagc
    34381 cattacccgg cagatcactc gggtggtgat catgggcggt gcatattttc atccagggaa
    34441 tatcactccg ttctcggaat tcaacctcta tgtggatcct gatgccgcgg agcaggtatt
    34501 caatgcggaa tgggatgacg tcacggcaat cggcctggat gtcacgcacc aaacggcact
    34561 cacccgcaat atgtgggaag cgattcctgc ggatgcaagc gggtctgccg gtctgatccg
    34621 ggcgatatca gagcggacct tcgaagagcg agcgcgctct ggcttcttcc tgcatgatcc
    34681 attggcggtg gcggtcgcgc tcgattcctc cctcatcacg gggcagcaca aggcggtgac
    34741 gatggacctc ctgggggaca cgcgaggcca gtcgaacgtt acgggcgtgg ggaacgtcct
    34801 cgttgcaaca gaggtccgag ccgatgtctt tgttcgccgt ttctgcgagg cgctgaacct
    34861 gccgtacgtt gagggcactg aggggcttgc caacgcggag tagcgggtgc aagaaaagtg
    34921 agcgtcacgg ttatcgtgac acccattttc ggttgctcag gactgccgga caactgccag
    34981 gtccagcata tcgttgaagt accggcgggt ttcgtcgtct ggaatcactt ccagtcgttt
    35041 ccgtgcctgc tcgacgtagg cccgggcaac atcttcggca cgctcgagcg cacctgagtc
    35101 acgaatctgt gcgacgacgc cgtcaatgcg tgcatcgttc gtctcgtcac cggagataat
    35161 cctctcgatt gtcctgcggg tgtcagtctc ggaggacagc ccctctgcga acatcatcac
    35221 aggtagggtg acaactccct ggcgaatatc atttccagca ggtttaccga tcgtatcggt
    35281 tcgttctcgc aggtcgagaa tgtcgtcaac gatctggaac gccatgccga gctctgtccc
    35341 gaagcgacgg agctcctgta ttgcatattc gggcgcttcg ccgataacgg ctcccatctc
    35401 ggcggcacca gcaaagaggg cagcggtctt tccataaatt cgctgttcgt aggattcccg
    35461 ggtctgtccg agatcgtgcg cttcgagggt ttcgcgaagt tggccatcac agatgtcggc
    35521 aagcgaggtg gcaaagatca tgaccacccg aggcacaccc gttgccgcag cgagaatcgc
    35581 gctctgggcg aatagatagt cgccgatgag gatgacagcg cccgagctga gttcagtgtt
    35641 cagggttggg ttgccgcgtc ggagagctgc gcgatcgatg ttgtcatcat ggatgagtga
    35701 ggcggtgtgg agcagctcca cccccgcggc ggcgatgatt gcatgctcgc ctggagcact
    35761 gaaggaccgc gttgccagca gcaagagcaa cgggcgcaac cgcttaccgc ctcccttgac
    35821 aatatcctga agcatcgttg agatgatagg gtagtcgacc ctggccgatt cctgcagctg
    35881 cttctcgacg aggtcgagct cacgcttcat ggattggaag acgtccgggg cgtgtacttt
    35941 cactggaact cccacatcgt cacagcacgg cgatgtctac ttgccatagc tgagacctgg
    36001 ctctcaatgg cgacgagtat caccgttcag agcggaacgt gtcaacggac ggacgtttta
    36061 tactgggtac gggtccgccc cgattggcta ggcctttcca atgaagacct caggcgacat
    36121 ctctataagc tcagtcgtaa ttctggagtg cttcggccac tcaggagacc atcgttgtca
    36181 aattaccccc gtgcgccgat tgctccgtta gccgcttccc atggaccagt caattcagag
    36241 cccgagcgac gctggcccgg agttcgctgg agtcacagcc tcatccttcc tgttgcactg
    36301 ctcctgatac ttatcaccct agccgtcgcc gagccccgca aagcattcct cgagctagag
    36361 gttgcgtcca acgtttccgg cgaggcgatt cctggtgcgg cggtcactat aggcaacaca
    36421 tcctatatcg ctgataagga cggcaagatt gttgtggagc gacccgaacg gggtgcctcc
    36481 ctctctgctg cctctgaagg gtatgagcct cttgtcgcaa agtttgcaag ctcggagcaa
    36541 cggtcggtca ctcttcagct taagccatcc atcgtccagg gagtgctgag agacggtggg
    36601 tcaggtgaag caatcgcgga cgctgacgtt gcgttgctgg atgcttcggg aaatgtggtt
    36661 gctgagacac gaaccgattc cagcggcgcg tatctcttca aggatttgcc agctcattcc
    36721 ctgctccgaa tcgacgctgg cgagtatggg gtcatccaaa aagaggttgg gcaacgtgcc
    36781 gtcgtcaatc tcgatctcga tcgttccgtt taccgcggca aagtcttaga tgatactggt
    36841 gcaccgttgc agggagcact cgtgcggtct ggggatagcc gtgtcgtcac cggcgccgac
    36901 ggcgagttca ccctggaagg ggtagggcag ggagcggagg tgcggatcac cgccgcaggt
    36961 tttcggtcca tgacgcggga catcggtagc gaagggctgg gagacattgc cctcgagcct
    37021 cagatgatca aggccgtata tgcaagctgg gcgctcctgg cagatccgga cggattggac
    37081 tcgctgatcg atatcgccaa cacgaccgag atcaatgcaa ttgttatcga tatcaagcaa
    37141 gacacaatct attacgacag ccaggtggag ttcttccgtc gggctgggac cgtggctcct
    37201 atctacgatg ctcgtgaagt ggttcgcagg ctggaggaaa acaatatcta tccgatcgcc
    37261 cgcctcgtgg tgttccagga tccactggtt gccgaggcct acccgcagtt cgccgttcag
    37321 gactccactg gcaagctatg gcgtaacgag atgggcgtgg cgtgggtgag cgcattcaac
    37381 gaggagttgt ggaacgccaa catcgagttg gcggtggagg cgatcgagct ggggttcgca
    37441 gaggtgcagt acgactacgt gcggtttccg tcggatggcg atctatcgac agcggatttc
    37501 ggacgtgagt acaccgccca ggcgcgggaa gacgcgatca ccgaatttat gaggcgcagc
    37561 tatgaagccg tccatcacgc aggagggctg ctcgcggctg acctcttcgg ctttgtgaca
    37621 atcgttgacg atgagcagta catcgggcag cggttcagcc ggctcttgcc atatctcgat
    37681 tacgtttgca tgatgatcta tccatcccac ttttcggaag gaaatatcgc cagcgcggcc
    37741 ggtcatccaa acgattttcc ctatgagact atctatgaaa gtctcgagcg ggcggagcaa
    37801 attgctccag gagcgaaaaa gaagtttcga ccctggttgc aggacttctc gtacggcagt
    37861 atgcgggagt atggcgccgc tgacgttcag gcgcaaatcg acgcggcgga agactatggc
    37921 gccagcgggt ggatgctgtg gggggatccg ttcaatgtca cagtagaggc gctccggcac
    37981 gatgattgag gcacatgacc tttcttccgc gagagtagag gccttgcgtc gcgcgcgcgt
    38041 caaccggtgg gttcgcctac tgcgtcccgt gcaatgggcc aagaatgggg tcgtgctcgc
    38101 ggcactcgtg ttcggaagag tctatgagga cccggccaag gtttgggcct cgctgggcgc
    38161 ggtcctcgct ttctgcctca ttagcagtgc cggatacatc ctgaacgatt ggctggatgt
    38221 tgagcgcgat cggcaccacc cattgaagcg ggcgcgtcct ctggcgtccg gtgctattcg
    38281 accacgctcc gcgctgattc tcgggggagc gctggcgaca accgctctga tctcctcgtt
    38341 gctcatatcg ccatcgctgg cgctcgtgat ctccgtctac gctctcctga tgtctgccta
    38401 tagtgtttgg ctcaagcgcg tggtcatcat cgatgtcttt gtcatcgccg caggatttct
    38461 cctgagggcg gtcgcaggag gcgtggcaat cagcgtacct atttctacct ggctcatgct
    38521 ttgcactgtg cttctcgctc tgttcctggg cttttgcaag cgtcgaaacg agattctgac
    38581 attgcaggag cgcgcgcagc tgcaccgtgt gtcgttgaga gggtatacgc cggaaatcct
    38641 ggatcagttc atcctgctta ctgcctcgag cacgctcatg gcatatgcga tgtacacgtt
    38701 cactgctgag tcggtaccga ccaatcacgc aatgatgatc actgtcccga tcgtggcgtt
    38761 cgcgctcttc cgctatctct atctggtgta cgggcgagcg ctgggcggtg ctcccgaagt
    38821 gctgctcttt cgggatgctc cgctgcttgg tgccattgtc ctctgggcat gcgcggtctt
    38881 catcgtcttc tggctcggct agtccagttg catgccatgt ggcactgctc gtgcatatgg
    38941 ggtgccgagc ggtggtccgc agcttaggca cttccttttc cccgtctacc accctcgcaa
    39001 cccaatgctt ccctttagaa cgcttatacg ggttgtagca acttgccgta cttttctgta
    39061 taatagctgc agtttgaacc ggacaaacca tcaacggcgt tcacatgtcc agttcctaag
    39121 acttagatcg accaagggag aattcatgat tgttgtccga cgggtgcgac cgcgtgaaca
    39181 agcgcggcgc cagaccgaga tcgatgccat gtatcgatcc ctgctcgcgg gccatcgtgc
    39241 agtcggcact ggccggcgcc gcacctggcg accgtccgtt gaggtgtatg aaaccgctga
    39301 gacgctggag gttgttgccg agatcgccgg catgcaggga gacgacatcg atatcgtgct
    39361 ggaaggggat gtcctgatga tccagggcac tcgcccggat ccggcaccat gtgaccaacg
    39421 tacctatcac atcgcacata ttgagtacgg agcatttgca acggaactac gaatcccgtt
    39481 tgcggtggat acggactccg cgaccgcttc ctacgagaat ggattcctac gaattaccct
    39541 gccgcgaacg aagggccgca ctattgttgc gacacgtgcg ggatcgaatt ccgatacggc
    39601 ggatgagcag agggacgcat gagcaatcct gacgagaaaa tgaccaacac gaacatcaac
    39661 gatgaaaccc aagacgacca agagcatcat gagttgcccg aggcggttct tccggaggcg
    39721 gaaaacgacg gcaacgacca agccgggcca gtagaagtcg tcgcggaagt cgtgggcgac
    39781 accgacgcgt ctccggagtt gccggaggaa gagatcctgc cgctgctgcc gctccgcggt
    39841 acggttgtgc tgccgatgac actcgtacca ttagccgccg cccaggagcg gtcgttgcgg
    39901 ctgatcgacg atgtgatggc cggcgagcgc acggtggcga tggtgatgca gaaggatgcc
    39961 cagctcgagg gcgcaggtcc ggatgacatc ctgtcgatcg ggacgattac cactattcac
    40021 cagatgatgc gcgttcccga cggatcggta cggctggccg tgcagggcgt cgagcgcatg
    40081 gaagttatcg aggttatcca ggaagagccg tatctcaagg ctcgcatccg gcgactgccc
    40141 gaggatctgg aggactctgt tgaggtgcag gcgctgatgc gcaatacgct cgaactcttc
    40201 cagcgcctgg tggccctcgt gcctcacctg ccggacgagt tggtgaccgc ggcactgaat
    40261 gtggacgacg atgcgcggca tctcgtctat ctcgtggcct cgaaccttcg gatggaacca
    40321 gaagagcgac agtcactgct tgagctgaac tcggtgacgg agaagctgca atcgctcaac
    40381 gccttcttaa ccaaggagct cgacgttctt gagctgggtc gaaagatcca gacagatgtc
    40441 caggaagaga tgagcaagtc gcagcgcgag tatttcttgc gcgagcagat gaaagctatc
    40501 cagaaggaac tgggcgagag cagtgaaacc gaagccgagg tcagcgagct ccggcagaaa
    40561 attgaagaat cgggaatgcc agaggaggca gcaaaggaag cgaaccgcga gttggatcgc
    40621 ctcagcaagc tgcccccggc cgccgctgaa tatggcgtca tcaagaccta tctcgattgg
    40681 ctgacgtctc ttccctggaa cgtttcgacc gagaacgaga tcgacatcaa gaagacgcgt
    40741 gagatcctcg acgcggacca ttacggactc gacaagatca aggatcgaat tcttgagtat
    40801 ctcgccgtac gcaagctgaa gctggatcaa gccacggacg acggcgaaac acaggcggct
    40861 catcgtgagc ccatcctctg cttcgtcggc cctccaggcg tgggtaagac gagtctcgcg
    40921 cagagcatcg cgaggggtct tggccgcgag atgacgcgca tgtcccttgg tggcgtgcgg
    40981 gacgaatcgg agattcgtgg acatcgccgc acatatgtcg gcgcaatgcc ggggcggatt
    41041 atccaggcga tccgtcgagc gggcagcaac gatccggtct tcgtgcttga cgaggtcgac
    41101 aagctgggca atgactggcg gggagatccg tcatcggcgc tgttggaggt tcttgatccc
    41161 gagcaaaaca acgatttccg ggatcactat ctcgatgtgc cgtttgatct ctcgaaagtc
    41221 atgtttatcg ctacggcgaa catgctcgac tcgataccgg ctccgcttcg cgaccgcatg
    41281 gagatcgtcg agctgagtag ctatacggac gatgagaagc tgcagatcgc gaagcgatat
    41341 ctggtgccga agcagatgaa ggcaaacggt ctgggcggcc aggagattga ctgggatgat
    41401 gatgcgctca tgagcatcat ccagcactac acccgggaat ccggcgttcg gaatctcgaa
    41461 cgcgagattg gaacggctgc ccggaagatc gcgaccatcg ttgccaaggg cgataacgcg
    41521 ccggaaacga tcgggaagcg ggagattcgg gactatctcg gccgcccgcg ctacttctac
    41581 gaggagcgtg agcagcggac ggcacagcca ggagtggcga tcggcgttgg tgtcaacagc
    41641 ttcggtggtg acatcatgtt cattgaagcg acgcggatgc cgggcaaggg aagcctgacg
    41701 gtgaccggtc agctcggcga tgttatgaag gagtcggcga ccgctgcctt ctccttcgtg
    41761 cgttcgaaga ccagggagct gggtatcgat ccggatttct acaaggattc ggatatccac
    41821 attcacgttc cggctggtgc catcccgaag gacggtcctt ccgccggcgt ggcgatgacc
    41881 accgcgctcg tttcgttgct tgccggtgtc tctgtccgtg acgatgtggc gatgactggc
    41941 gagatcaccc tgcgtggcca agtgctgccg gttggcggta tcaaggagaa ggcgctcggt
    42001 gcgcgtcgtg ccggtatcac gacctttatc ctgccgcgac ggaacgaggt ggatctcgac
    42061 gacttgcctg aggagctgaa ggacgaaatg atcttcgtgc tcgccgatac gctggacgag
    42121 gcgttgcaga cctcgatgcc ggaggaattc cacggcgtca gggcggcctc gacgcctgtg
    42181 gcaatcgcaa gctagccact gctcccatcc gatacacaac agaaacggcg cgcccccatt
    42241 gggacgcgcc gttttcgtag gcaccggcga ttgctacttc ccggccttct tgtccgctgc
    42301 cgcacgaacg agggcctcga agattgctgc ctggtccggg tattcctctg caagcatctc
    42361 ggggtgccac tgaacggcaa ggccaaaagt catcctcggg tgccacatag cctctacgac
    42421 gccatctggt gttatggcca cgacctctaa ctcctcggcc gggtccttca ccgcctggtg
    42481 atggaacgag ttcgtttgca tttttgtatc gccatgaatc cggtaaagaa ggttgtcccc
    42541 ctcagtaatg gtgattgtat gggagcgggc gtcccgcatc ttgccctccc tgtgctgccg
    42601 atgctgctgg gctccgggta gctggtcggg gatatcctga atgagtgtgc caccaagggc
    42661 gacgttgacc gtctgaatgc ctcgacagat gccgagcatg gggatatcgc gctcaactac
    42721 cttcggaatg gcctgtaact cgaaggcatc gcgctcgtca tcgaagccat ccgccgcggg
    42781 atgcttttcc tcattccagt agccaggatc aatatcaccg ccgccactga agacaatgcc
    42841 gtccactgca tcaagggtct cgtcaatcgc accggtatga agtgggagaa ttacaggtac
    42901 tccgccagct gcgatgatgg ccctggtata ggtatcaccg agcgcatatc ggcggaaatg
    42961 gccgtggtcg aaggtggact cactggggga gggggagatg ccgattaccg gtttcacacg
    43021 taactccaga cgacagaggg accgcacgct ttcagactaa accgcaacgc cacaagtata
    43081 cgtaggagcg gtcgatttca ttcccacgtt cccacccccc gttcctagat tcttgcgcgc
    43141 ggcccgtcga tggggcagcc ttgacaggtg gcaggggggc agctatagtt gctctccgct
    43201 gctgagaaat cggtggcgcg gaccctgacg agaggttcct ggaaacaggg tcttgacagc
    43261 gggtggggga gcgggtacac ttgctctccg ctgctccggg aacgaccggg cagcaggtcc
    43321 ggcgcaagcg gctctttcgg gaggggcttg acacggaccg gaccgggcag gtatactact
    43381 tgtccgctgc tgagagatca g
//
LOCUS       DASYWM010000165         9991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_287968, whole genome shotgun sequence.
ACCESSION   DASYWM010000165 DASYWM010000000
VERSION     DASYWM010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287968"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..375)
                     /locus_tag="VGR29_06780"
     CDS             complement(115..375)
                     /locus_tag="VGR29_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073335.1"
                     /translation="MTVRQKVRELGPSDFKGRPVTEEELAAWAATTPEQQALFKGEMT
                     PTFRWLLDRYGERFPEWLETAVAVYREQERLDWEGGADGDTE"
     gene            592..1863
                     /locus_tag="VGR29_06785"
     CDS             592..1863
                     /locus_tag="VGR29_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073336.1"
                     /translation="MLAQLLARLLLRRAYRDGTVIVAAPTFKPQALISRDRLLDRLLH
                     NPLTSLITITHDRSTVAVGKARAVFLSAARTANARGQTADIALIANESQDIEPAVWDA
                     VFDPMAASTNATTIFLGTVWSRETLLARQMHHLRSREAVDGRQHLFLVPWQRVAADVP
                     AYGERVRARMEQLGERHPFIRTEYYLEELDGENSLFPPQRIAQMQGEHLRQHRATSGK
                     RYALLLDVVGEEESGGDQFSFGMEHASRRDSTALTVVEVDTPSRHDGRTCYRVVDRRA
                     WTGVRHSMLHEHLVNLARNVWRASWVVVDATGIGAGLASFLEASLKRSGIKVVPFLFT
                     SASKSRLGWDLVGLIESGRLKEYADDGDELTRLLHAQLRATTYEIRNGPGNLLCWSVP
                     DREGHDDLVMSIALVAVLDGLDMRPRIAMGS"
     gene            complement(2281..2574)
                     /locus_tag="VGR29_06790"
     CDS             complement(2281..2574)
                     /locus_tag="VGR29_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV2073337.1"
                     /translation="MKTAYVYIMANQTRTMYTGVTNDLQRRVWEHKTGFDPNGFTSRY
                     KLTRLVYFAEFSRFGDAIAYEKQIKGKSRAWKMGLVEERNPRWSDLAWDWFQD"
     gene            complement(2924..3520)
                     /locus_tag="VGR29_06795"
     CDS             complement(2924..3520)
                     /locus_tag="VGR29_06795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV2073338.1"
                     /translation="MQTIVQIETKFGVIRVVLDEDAAPITATNFLAYVDEGLYDGGMF
                     HRTVRLDNQSNKNLKRETIGKGIDPKADRDQLPNDEVAIEVIQGGVNRDREDELGDPI
                     PLERTSETGLSHVDGTISMARLTPDSAVSDFFICINDQPSLDYGGKRNPDGQGFAAFG
                     HVIEGMDVVRTIHAQPADGQRLDPPVTINRIARESDER"
     gene            3982..4821
                     /locus_tag="VGR29_06800"
     CDS             3982..4821
                     /locus_tag="VGR29_06800"
                     /inference="COORDINATES: protein motif:HMM:NF042870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HEV2073339.1"
                     /translation="MDPIAFQKKWRTASVNLKERPASQSHFNDLCAMLGVQNPIEADP
                     VSDTYTLEKGAEKAGGGGGWADVWYKGRFAWEYKGLGRDLDAAYLQLLRYKDALENPP
                     LLVVCDLDIIEIRTNFTNTTQRTERITLDEIADAGKCAMLKRVWTDPDWFRPDLRPED
                     VTAEAAARFSEIARGLYSRGIAPARAAHVLMQLLFCLFAEDIGLLPNRIFSKMLDFGV
                     RYPARFQTEATALLEAMNSGDVEAYEKIPHINGGLFRRVDVPELAEPEICILRQAAKL
                     DWS"
     gene            4923..5390
                     /locus_tag="VGR29_06805"
     CDS             4923..5390
                     /locus_tag="VGR29_06805"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEV2073340.1"
                     /translation="MVDPVVMTPLRRRWNEVRAEADALLAAAESAKIAATRTRQRNAF
                     TAKIDAFLAELREVRVLDPACGSGNFLYIALERLLNLEKEVRLYRAMAGFPLGFPEIS
                     PQQVLGMEINEYAQELAQVAIWIGYLQWMIRNGFGWSEPVLDELETIRLQEPC"
     gene            5408..5728
                     /locus_tag="VGR29_06810"
     CDS             5408..5728
                     /locus_tag="VGR29_06810"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEV2073341.1"
                     /translation="MSETIWPHADFIIGNPPFLGGKRLRAELDDDYVNMLFNVYNGRV
                     ARESDLVCYFFEKTHAQLEHDGAERAGLLATNSIRGDANREVLKRTSRAVRFTWRGPT
                     SRGF"
     gene            5890..6093
                     /locus_tag="VGR29_06815"
     CDS             5890..6093
                     /locus_tag="VGR29_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073342.1"
                     /translation="MGDTKGGPFDISNDLAQQFLSLPINPNGRPNTDVIRPWVNGLDI
                     ARRPRNMWIIDFGVDTPEHDATL"
     gene            6115..6783
                     /locus_tag="VGR29_06820"
     CDS             6115..6783
                     /locus_tag="VGR29_06820"
                     /inference="COORDINATES: protein motif:HMM:NF042871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HEV2073343.1"
                     /translation="MTTVKPVRGKSKSTTDKWWIHERPRGEMRTALRSTSRFLITPRV
                     ATYRLFAWMDAATLPDSATIAFARQDDYFFGVLHSRAHEVWSLRVGTSLEDRPRYTPT
                     TCFETFPLPWPPGTEPVDDPRVIAIGEAAKRLNELRERWLNPEGANEAELKKRTLTNL
                     YNQKPTWLWNAHAALDRAVWAAYGWDDPDPTTVDEDTILSGLLALNGERASTAEADAA
                     PYSG"
     gene            7019..7477
                     /locus_tag="VGR29_06825"
     CDS             7019..7477
                     /locus_tag="VGR29_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2073344.1"
                     /translation="MALLNGIHHLAILTADMDRLIAFYERVFDARLLLDMEEEGLRHA
                     FIELEPTTVLHPFQIPGVEVPQGPVPMFTRGRLDHFALNASSEEAFREVRQRVIAAGA
                     GDGTVTDVGPILSLTFTDPDGAEHEVTWSKPGVTFDRSQTRALWTTVEME"
     gene            7928..8350
                     /locus_tag="VGR29_06830"
     CDS             7928..8350
                     /locus_tag="VGR29_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073345.1"
                     /translation="MTLHTRVHPRTSLLLAALLPALATAAASIPHVLNTPEEGSPAQG
                     WLMLSIAPLVALLVNGRWPAATNVSRAFLVALPQLLVVISLARLDVWLDVRSGYLLRN
                     SGEEAMAYGIGTTLGVIMGIVLVVLVASAGRLGALLAE"
     gene            8474..9598
                     /locus_tag="VGR29_06835"
     CDS             8474..9598
                     /locus_tag="VGR29_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073346.1"
                     /translation="MRPVLPSLVLVVAVLVATVPHPAFAHLDATVGRLAFASSLGQPD
                     AFGFDLYTVATDGTDLTRLTRFGEEGMLAQHPTWSPDGNRIAFGARDLSRAGLGIHLM
                     DADGANLLQLTDAGDCNHFDFFAFHGPDWSPDGNHIAFAADLNRSCGEPDLYLADASG
                     EDIAQLTSLAEQGHSSWMPDWAPDGTRLVFVSGPGGGIGDCYVVSASGGEPMRFGEPG
                     CQFPAWSPDGTMIATISRDGLCLVPVDEGKRTCPMSPTGGMPSGMLWNHAWSPDGTKI
                     LFEMLEMPEGGVGESPPSAVYVLDVATGKLTRIDTHVAFAVAPVWSADGSHIAFVARL
                     DDQPGTDLYIAAPDGGDLVRVTTVGDDGAFVADPAWDPAG"
     gene            9701..>9991
                     /locus_tag="VGR29_06840"
     CDS             9701..>9991
                     /locus_tag="VGR29_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2073347.1"
                     /translation="MSDVTSSRSHWRGAHHIALMTGDMDATVCFYHGLLGMPLVGGGG
                     VNPMHGRHYLFDAGEFLVGFFWEVTWTPMGAEKGHAGWTFADPDPVPSANEIH"
BASE COUNT         1827 a         3211 c         3032 g         1921 t
ORIGIN      
        1 taagagcctt ctggccaggg tcctggaagc tcctggtcga tcgtgagctt gatgaagggc
       61 cgatcctcaa cgctgactgc ttccctgacc gtgccgccga tcgatgggta cgcctcactc
      121 cgtgtcgcca tcagctcctc cttcccaatc cagccgctcc tgttcccggt acactgccac
      181 tgccgtttcg agccactcag ggaaccgctc tccgtaccga tccagcagcc accggaatgt
      241 cggggtcatc tcacccttga atagggcctg ctgttctggc gtagttgctg cccacgctgc
      301 taactcctct tcagtcactg gccgaccctt gaagtcactc ggtcccagct ccctgacctt
      361 ctgccgtact gtcatgtaag ttctccctgc cctctcgggc gactgatggg gcaagcagaa
      421 agcccccgtg ccatcaatga tgacggcatc ggggacgtta ttcaatagtt tataaggcct
      481 gtttcgcaac cagttttatt ttgggaaaat aactcccccg tatttacggt cttctttttc
      541 caatctgacg gtccccagat aagctgtagg ggtgttgtat tgagaaaagt aatgctcgcc
      601 cagcttcttg cccgcctgct gctccgccgg gcctatcgcg acggcaccgt catcgtcgcg
      661 gcgccgacct tcaagccgca ggcactcatc agccgcgacc gcctgctgga tcgcctcctc
      721 cacaatcccc tcacctccct gataaccatc acgcacgacc gctccaccgt cgccgtcggc
      781 aaggcgaggg cggtcttcct atccgccgcg cggaccgcga acgcccgtgg ccagaccgcc
      841 gacatcgccc tgatcgcgaa tgagagccag gacatcgaac cagccgtttg ggatgccgtc
      901 ttcgacccga tggccgcaag cacgaacgcg acgacgatct tcctcggcac cgtctggagc
      961 cgcgagacgc tcctcgcccg gcagatgcac catctccgta gccgggaagc ggtggatggc
     1021 cgccagcacc tttttcttgt gccgtggcag cgggtcgcgg cggatgtccc ggcctacggg
     1081 gagcgggtcc gcgcccggat ggagcaactc ggcgagcgac atcccttcat tagaacggag
     1141 tattatctcg aagagctcga tggcgagaac agcctcttcc cgccacaacg gatcgcccag
     1201 atgcaggggg agcacctccg ccagcaccgg gcgacatcgg gcaagcgata cgccctactg
     1261 ctggatgtgg tgggcgagga ggaatccggt ggcgatcagt tttccttcgg catggagcac
     1321 gcatcccgcc gggatagcac cgccctcacc gtggtggagg tggacacccc ctcccgtcac
     1381 gatggccgca cctgctacag ggtggtcgat cggcgagcat ggacgggcgt ccgccactcc
     1441 atgctccacg agcacttggt caacctggcg cggaacgtct ggcgagcaag ctgggtggtc
     1501 gtcgacgcaa ccggcatcgg cgccgggctt gcctccttcc tcgaggcgtc gctgaagcgt
     1561 agcggcatca aggtcgtgcc ctttctcttc acgagcgcaa gcaagagccg ccttggctgg
     1621 gacctcgtcg ggctgatcga gagcggacgg ctcaaggagt acgccgacga cggcgatgag
     1681 ctgacgcggc tgctccatgc ccaactccgg gcaacgacct atgagatccg gaacgggccg
     1741 ggaaacctgc tgtgctggtc ggtgcccgac cgcgagggcc acgacgatct tgtcatgagc
     1801 atcgcgcttg ttgccgtgct cgatggcctc gatatgcggc cacggattgc gatggggtcg
     1861 tgacgggagc ggcgagagcg gatcaggaag gggcgatatc cgggataagg ggggatagta
     1921 cggggcgctc atggagatcg ctcatgcttt tcgccctgcc aacgttgccc attcagggtt
     1981 ccgcgcgaaa cggtggaggc caaacgcgta tttccgttga gcgtcatcga gcgagacgtg
     2041 agcgtcgtcg acgcgcttcg cgtaggattc atcgcccttc gggctctgaa tgacggaggg
     2101 tgtgtggtga aatgacgaac tgcatcacgc agcgctgcat gatgctcact ctggtacaac
     2161 acactaaccg tcattcagag cgcggcaagg gctcagccaa gggctcgctg cccctccctg
     2221 cgacaccgtc attcagagcg aagcgatgaa tcccacgcga agcggatgac gagggggcgg
     2281 ttagtcctgg aaccagtccc aagcgaggtc actccagcgc ggattgcgct cctccaccag
     2341 gcccattttc cacgcccgtg acttcccttt gatctgcttc tcataggcga tcgcatcgcc
     2401 gaaccgggaa aactccgcga aatacaccag cctggtcagc ttgtatctgg atgtgaagcc
     2461 attgggatcg aagcctgtct tgtgctccca cacccttcgc tgcaggtcgt tggtaacgcc
     2521 ggtgtacatg gtccgggtct ggttggccat gatgtagacg taggccgttt tcatgggacc
     2581 tccgtggaag cctcgtccgc gcttatggcg attcgctgcg tgcgctttcc gatctcatga
     2641 catcttggtg atgagcaccg cttcgcgttg gattcacatt gggcggtccc aacgggaccg
     2701 agcgggagcg gccccatggc tgagcccgga gggcgaaaag cggtagcgag cctttgcttc
     2761 gctctgaatg acggttggaa gctgtgggcg tgccgcatgc gctcaaaggt ttcgctcttg
     2821 cctctgtccg gtgcgatcat ggagatcgtc cgtacgcagg tggcgcctgg ttcatggtgt
     2881 aggggctgac cccatagggt ggtcagcccg attggcggcc gccctaccgc tcgtcgctct
     2941 cgcgggcgat gcggttgatc gtcaccggtg ggtccagccg ctggccatcg gccggctgcg
     3001 catggatcgt gcggacgaca tccatcccct cgatcacatg cccgaacgcg gcgaacccct
     3061 gcccgtccgg attccgcttt ccaccgtaat cgagcgatgg ctggtcgttg atgcagatga
     3121 agaaatccga gacagcactg tccggcgtaa ggcgcgccat cgagattgtg ccatcgacat
     3181 gcgatagccc cgtctccgat gtccgctcca gcgggatcgg gtcccccagc tcatcctcgc
     3241 gatcccggtt cactccaccc tggatgacct cgatcgcgac ctcgtcgttc ggcaattgat
     3301 cccgatccgc cttgggatcg atccccttgc cgatcgtctc ccgcttcagg ttcttgttgg
     3361 attggttatc gagccggacg gtgcgatgga acatcccgcc gtcgtagagc ccctcatcga
     3421 cataggccag gaaattggtg gctgtgatcg gcgcggcgtc ttcatccagc accacccgga
     3481 tgaccccaaa cttcgtctcg atctgaacaa tcgtttgcat ctctctcctc ttccgcgcga
     3541 tgcccctcac cgcgctgcac cgtgacatca tggcggaaaa ggcggaattt gtcccgaacg
     3601 acgttctgca ttggccgtat acgggcgaac tctatgttgc cccgggtacc acgataggtg
     3661 gacagagagc cactccaaaa ggacgcccgc gatagccgtc cttacacaat aaacgtcgtc
     3721 atttagagcc cttcgggccc tgaatgacga agcaattctc atgtgagggc agaccccggc
     3781 tggacgagga cttcccggac tcaatgacct cacagcgtac gggcgatctc catgatcgcc
     3841 ccggttataa ggaaagggcg gacagagagt ccgcccgtac gaaacctcct cccgcgaccc
     3901 caatgccagt cacccgcatg catccccaaa ccgcctatac tccccagaca aacccatccg
     3961 acagcacccg gagcagcccc cgtggacccg atcgcgttcc agaagaaatg gcgcaccgcc
     4021 tccgtcaacc tcaaggagcg gccggcctcg caatcccact tcaacgacct ctgcgcgatg
     4081 cttggcgtcc agaaccccat cgaggccgac ccggtcagcg acacctacac cctcgagaag
     4141 ggcgccgaaa aggccggtgg tggtggcggc tgggcggatg tctggtacaa gggccgattc
     4201 gcctgggagt acaaggggct tgggcgtgat ctcgatgccg cctacctcca gctgctccgc
     4261 tacaaggacg cgctggagaa ccctcccctg ctggtcgtct gcgacctcga catcatcgag
     4321 atccgcacca acttcaccaa caccacgcag cgcaccgagc ggatcaccct cgacgagatc
     4381 gccgacgccg gcaagtgcgc gatgctcaaa cgggtctgga ccgaccccga ctggttccgg
     4441 cccgacctcc gcccggaaga cgtcaccgcc gaggccgccg cccgcttcag cgagatcgcc
     4501 cgtgggttgt acagccgggg catcgcgccg gcccgcgccg cgcacgtcct catgcagctg
     4561 ctcttctgcc tcttcgcgga ggacatcggc ctgctgccca accgcatctt ctcgaagatg
     4621 ctcgacttcg gggtccgcta tccggcgcgc ttccagaccg aggcgaccgc actgctggaa
     4681 gcgatgaaca gcggcgatgt cgaggcttac gagaagatcc cgcacatcaa cggcggcctc
     4741 ttccgccggg tcgatgtgcc ggagctggcc gaaccggaga tctgcatcct gcggcaggcg
     4801 gcgaagctcg actggtcctg aacgagccgg ccatcttcgg cacgctcttc gagcgctcgc
     4861 tcgacccgga gaagcgaagc cagctcggca cgcactacac gggacgcgcc gacatcgagc
     4921 gggtggtcga cccggtggtg atgaccccgc tgcgccgccg gtggaatgag gtccgcgccg
     4981 aggcagacgc cctgctggcg gcagcggagt cggcgaagat cgccgcgacc aggacgcggc
     5041 agcgcaacgc cttcacggcg aagatcgacg ccttcctcgc ggagctgcga gaggtacgcg
     5101 tgctcgaccc ggcctgcggc agcggcaatt tcctctacat cgcgctggag cggctgctga
     5161 acctggagaa ggaggtgcgg ctctaccgcg ccatggccgg gttcccgctc ggcttcccgg
     5221 agatcagccc gcagcaggtg ctagggatgg agatcaacga gtacgcgcag gagttggcgc
     5281 aggtcgcgat ctggatcggc tacctgcagt ggatgatccg caacggcttt ggctggagcg
     5341 agccggtgct cgacgagctg gagacgatcc gcttgcagga gccctgctga cacaggtaga
     5401 cggccacgtg agcgagacca tctggcccca tgcagatttc atcatcggca acccgccgtt
     5461 cctgggcggg aagcggctcc gcgccgaact ggacgatgac tacgtcaata tgctcttcaa
     5521 cgtctataac gggcgggtgg cgcgagagtc tgatttggtc tgctacttct ttgagaagac
     5581 acacgcgcag ctagagcacg acggagctga acgcgctggc ttgctggcca cgaattcaat
     5641 ccggggtgat gcgaatcgtg aagtgctgaa acggacaagc agagcggtaa gatttacatg
     5701 gcgtggtccg acgagccgtg gattctgaag ggcgcggccg ttcgcatttc gatcatcggc
     5761 ttcgatgacg gcgcagagga tgcgaaacac ctagatgacg agcccgtcgc atcaatcaac
     5821 agtgacctaa cgtccacaac tgatattaca catgcttcgc ggcttcatga aaacgtcgga
     5881 attgctttca tgggagacac aaagggcggc ccctttgaca tttccaacga tcttgcacag
     5941 cagttcctca gccttccgat aaatcccaat ggccgaccca ataccgatgt cattcgccct
     6001 tgggtaaacg ggcttgatat tgcacgacga ccaagaaata tgtggattat cgactttggc
     6061 gttgacacac cggagcacga tgcgacccta taagaaactc cttatgaata catactgaca
     6121 acagttaagc cagtacgagg taagagcaaa tcaaccacag acaaatggtg gatccacgag
     6181 cgccctcgtg gagagatgcg aacagcgctc cgttctacat ctcgcttcct cataacgccg
     6241 agagtcgcaa cctatcgact cttcgcttgg atggacgcgg caacgttgcc ggacagtgcc
     6301 actatcgcct tcgcgcgaca ggacgactac ttcttcggtg tgctgcactc ccgggcgcat
     6361 gaggtttggt cgctgcgagt ggggacatcc ctggaggatc ggccgcgcta caccccgacc
     6421 acctgcttcg agactttccc ccttccctgg ccgccgggca ccgagccggt cgacgatccg
     6481 cgcgtcatcg ccatcggcga ggcggcgaag cgcctcaacg agctgcgcga gagatggctc
     6541 aaccccgagg gtgccaacga agcagagctg aagaagcgaa cgcttaccaa cctctacaac
     6601 cagaaaccga cgtggctgtg gaacgcccat gccgcgctcg accgcgccgt ctgggccgcc
     6661 tacggctggg acgatcccga tcccactacc gtcgatgagg acaccatcct ctccggcctc
     6721 ctcgccctca acggggagcg ggcgagcacg gcggaggccg atgctgcccc gtacagtggt
     6781 tgagcaccgt gccggagcgg ggttgcgacg aaggcaggag ggtggcctcg tatccttttg
     6841 tccgacaagc tgctatcctc gcttcacgat ttacctaacc ggtcgttgca gaatagcggt
     6901 atgtccacga atcgagccgt tccgggtgcg tccgggttcg catggccgca ggttcctgat
     6961 cccggcgaac gttcccgcat cctggaccga tgccatacgc gagcaggcag gagagttcat
     7021 ggcattactg aacgggatcc atcatcttgc gatcctcacg gccgatatgg accgcctgat
     7081 tgcgttctac gaacgcgtct tcgatgcacg gctccttctc gatatggagg aggaggggct
     7141 gcgccatgcc tttatcgaac tcgagcccac cacagtcctg catcccttcc agattcccgg
     7201 cgttgaggtg ccccaggggc cggttccgat gttcacccgt gggcggcttg atcacttcgc
     7261 gctgaacgcc tccagcgaag aagcgtttcg tgaggtgcgg cagcgcgtca tcgcggcggg
     7321 tgctggcgac gggacggtca ccgatgtcgg tcctatcctg agcctgacct ttaccgaccc
     7381 ggacggtgcc gaacacgagg tgacctggtc caagcccggt gtcacgtttg accgcagcca
     7441 gacccgggct ctgtggacca cggtggagat ggagtaggaa ctatgtgcac caatgaaatc
     7501 tgtcctcttg ccgagcctgt aatgggaccg gcttacgaac aggaagcggt ggtgcaagcg
     7561 tcctagtgat gacaacggat gtgttcagcg gtctgccatg aggggggcgc ttgccactgg
     7621 ccgtcgagtg cacaaggtcg ggagcacctg cccctgttaa cttcgacggg gttccccttc
     7681 ggcaatacgc cctcctatcc cgttagtccc tgccttgcgc aaaatgcgta actggccaaa
     7741 tacgtacttg tactgaggtc aggttcctgt cactggtcta gattctggaa gaagggttct
     7801 gctagatgtc ccgccagcaa ccctggcaga actttctcgc gagagccaac caggctggag
     7861 gccgttggcc atatcgacgc cgtatgatgg agctatttca ccggcgcacg gaaaggcgga
     7921 gcagagagtg acgcttcata cacgtgtcca tccccggacc agcttgctgc ttgccgccct
     7981 cttgcccgca ctggccaccg ccgcggcgtc gatccctcat gtgctcaaca cgcccgagga
     8041 gggttcgcca gcacaaggat ggctgatgct gtccatcgcc ccccttgtgg cactgctcgt
     8101 gaacgggcgc tggcctgcag cgacaaacgt gtcccgagca ttcctcgtcg ccctgcccca
     8161 gctcctcgtt gtcatctctc tggcacggct ggatgtctgg ttggatgtcc ggagcgggta
     8221 cctgttgcga aactcgggcg aggaggccat ggcttacgga attgggacga ctttgggtgt
     8281 catcatgggg atcgtgctcg tggttcttgt ggccagtgcc ggaaggctgg gtgccttact
     8341 cgcagaatag cccccgctgc tcctccgggg ggctccagga gtggtcatcg gtgcaggcag
     8401 tcgcagaaat tgccgctcca gcacatggaa cagcctatgc ccgcggtcgt gggtgaagct
     8461 aaaggagttc ctcatgcgtc ccgtgctccc gtccctcgtc cttgtggttg ccgtgctcgt
     8521 ggcgaccgtc ccgcatccgg cgttcgcaca cctcgacgcg accgtcgggc ggctcgcgtt
     8581 tgcctcgagt ctcggccaac cagatgcctt tggcttcgat ctctacaccg tcgccaccga
     8641 cggcaccgac ctcacccgcc tgacccgctt tggcgaggag gggatgctgg cccagcaccc
     8701 gacctggtcc ccggacggca accggattgc cttcggggcc cgcgacctct cccgtgccgg
     8761 tctcggcatc catttgatgg acgccgacgg ggcgaatctc cttcagctca ccgacgccgg
     8821 cgattgcaac catttcgact tcttcgcctt ccatggacca gattggtcac ctgacggcaa
     8881 ccacatcgcc ttcgccgctg accttaatcg ctcctgcggg gagccagatc tgtacctggc
     8941 cgacgccagt ggggaggaca tcgcccagct cacatccctc gccgagcagg gacactcctc
     9001 ctggatgccg gactgggccc ccgacggcac ccggctcgtc ttcgtttcgg gtcccggcgg
     9061 aggaatcggg gactgttatg tggtctccgc gtcgggcggt gaaccgatgc gcttcggtga
     9121 gccggggtgc cagtttcctg cctggtcgcc ggatgggacg atgatcgcca ccattagtcg
     9181 tgacggtctc tgtctcgtcc cggtggacga aggcaaacgt acctgtccta tgtcgccgac
     9241 ggggggcatg ccatctggca tgctctggaa tcacgcctgg tcgccggatg ggacgaagat
     9301 cctcttcgag atgttggaga tgccggaggg aggcgttgga gagtctcccc cgtccgcggt
     9361 ctatgtgctg gacgtggcga ccggcaagct gacccggatc gacacccatg ttgcattcgc
     9421 cgtcgcgcca gtgtggtcgg ccgacgggag ccacatcgcc tttgtggccc gcctcgacga
     9481 tcagcccggc accgacctct atatagcggc ccccgacggt ggtgacctcg tccgggtgac
     9541 gacggtgggg gatgatgggg cctttgtggc cgacccggcc tgggatcccg cgggctgatg
     9601 cggcacgtat atgcctcttc aggtctgtgc cagtcacacg accccgttgg ttgtctctgg
     9661 agtagcaatg caacgggaac cactcacgga ggagctaacg gtgagcgatg taacgtcatc
     9721 aagatcacac tggcgtggtg cacatcacat tgccctgatg accggcgata tggacgcgac
     9781 cgtgtgcttt taccacgggt tgctgggcat gccgctggtt ggcggcgggg gtgtgaaccc
     9841 catgcatggc cgccattacc tcttcgatgc gggggagttc ctggtcggct tcttctggga
     9901 ggtgacctgg acaccgatgg gagccgagaa gggtcacgcc gggtggacct tcgccgaccc
     9961 cgaccccgtc ccttcggcga acgagatcca c
//
LOCUS       DASYWM010000166         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_363222, whole genome shotgun sequence.
ACCESSION   DASYWM010000166 DASYWM010000000
VERSION     DASYWM010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363222"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1438)
                     /locus_tag="VGR29_06845"
     CDS             complement(188..1438)
                     /locus_tag="VGR29_06845"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2073348.1"
                     /translation="MTMTEETLEASMKVADGKPKSGKGLQGQTFLVGDEVYLRTFRVG
                     DHKNATSWRNSVFPKSAALVKQWIKEELPKEGKAQRQHLAIVRKEDDTIVGSVLIKYD
                     DLACHVVPYVDPLYGNAGARWKAEAIRLLVEWLVDENHRPVVHVTLAANEATVIEAVQ
                     AIGMRECARFRECYLIDGKRVDKIQLEYLNKGWVETLGDPNEMGLERTGTGQPRPVPP
                     RVTVEGDPPKNAMMIGKRVYLRPEEKAETKLIAKWSRQETETFFDIGRHLMSSVVHAS
                     WIDGQYEKDKPGWIWFAVCLRETDKVIGAVGLLRVDYVNLTAETGSIFHQKEYRGSGY
                     GSEAKQLLLEYAFDVLGLHMLESWVLFPNTRSAAALRKQGYREAGRAHWIYPHNGRFD
                     NFVVFDLLAKEWRAMPRAEWDAKA"
     gene            complement(1435..2676)
                     /locus_tag="VGR29_06850"
     CDS             complement(1435..2676)
                     /locus_tag="VGR29_06850"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2073349.1"
                     /translation="MSATATETESKQETPAPGTGHVGQAFLVGESIYVRPVEEEDADY
                     GMSWFGSVIPKSVDRIKKWIKEEMKEEHNTTHHVILRKADDRPVGSVKVERWGYAVWL
                     TPYADRLFGEQGDRWIVEALGLVIPWVIDEQHRVIAYVYDIPADKPIIREGLTRIGAR
                     ECGRFREKIRRGSSYIDAYQYDYPNKQWIETLGDPADIELERTGTGEPRPVSPPVTVE
                     GTLPPNAMRIGARVYLRPLQKSDAKLVVKWSRRETEPFWSNGRPLYTETSVQKWFEGL
                     QKEEPQDWVRFAVCLRENDKMIGAVGIDGIDYQNRCAESESEIIRPEYRGGGYGSEAK
                     HLLFDYAFNTLGMHSLQSYVFFPNTRSAAALRKQGYREAGREHWLLPVSGRLQNFGTY
                     DLLADEWRAMPRREVTETSTT"
     gene            complement(2862..>3105)
                     /locus_tag="VGR29_06855"
     CDS             complement(2862..>3105)
                     /locus_tag="VGR29_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073350.1"
                     /translation="HNRHPIVYSGRSTPFGELERAALPHRSRHVGTSMTPDLSGSSPP
                     MSSPRCIGPGLVGKGGGAMMLQLVNTDTHAGVMALA"
BASE COUNT          528 a          993 c          932 g          652 t
ORIGIN      
        1 ggtgcgacac cgaggatctt gatgcgctgg tgtagggttc ctcgacacgg attcgttcgc
       61 tcggggattt ttgctccact gcgcgggcat gagcaggccg caaaccgcaa catgcgtggc
      121 gaaggccgtc ggtgtggtgg atgcgatcac acaggatcgc acccaccacc gtcggtcagg
      181 atctgtgcta cgccttcgcg tcccactcgg cgcggggcat tgcccgccac tccttggcga
      241 gcaggtcgaa gacgacgaag ttgtcgaacc ggccgttatg gggatagatc cagtgcgccc
      301 ggcctgcttc cctgtatccc tgtttccgca aggctgcggc agagcgggta ttcgggaaga
      361 gcacccacga ctcgagcatg tgcaaaccga ggacatcaaa cgcgtactcc agcagcagtt
      421 gcttcgcctc ggagccgtat ccgcttccgc ggtactcctt ttgatggaag atactgccgg
      481 tttccgcagt aaggttgacg tagtccaccc gtagcaagcc gacggcgccg atgaccttgt
      541 ccgtctcccg caggcagacc gcgaaccaga tccagcccgg cttgtccttt tcgtactgcc
      601 catcgatcca gctggcatgg acgaccgagc tcatgaggtg gcggccgata tcgaagaagg
      661 tctcggtctc ctggcgggac catttcgcga tgagcttcgt ttccgccttc tcctcaggcc
      721 gcagatacac ccgcttgccg atcatcatcg cgttcttcgg cgggtcgccc tccacggtga
      781 ccctcggcgg cactggacgt ggctgccccg taccagtacg ctcgagcccc atctcgttcg
      841 ggtcgccaag cgtctccacc cagcccttgt tcaggtactc gagctggatc ttgtctacgc
      901 gctttccgtc gatcaggtag cactcgcgga aacgggcgca ttcccgcatg ccgattgcct
      961 gcaccgcttc gatgacagtg gcttcgttcg cggcaagcgt gacatggacg acgggccggt
     1021 ggttctcatc cacgagccac tcgaccagca gcctgatggc ctccgccttc cagcgggcgc
     1081 cagcattccc gtagagtgga tcaacgtagg gcacaacgtg gcaggccagg tcgtcgtact
     1141 tgatgagcac cgacccaacg atggtgtcat cctccttgcg gacgatagcg aggtgctgcc
     1201 gttgcgcctt gccttccttt ggcaattctt ccttgatcca ctgtttcacc agcgccgcgg
     1261 atttcgggaa gacggagttg cgccatgatg tggcgttctt gtgatcgcct acccggaagg
     1321 tgcgcaggta gacctcgtct cccaccagga aggtctgacc ctgtaggccc ttgccggact
     1381 tcggctttcc gtcggcgacc ttcattgacg cttccaaagt ttcttctgtc atagtcatgt
     1441 ggtactcgtt tctgtgacct cgcggcgagg catggcccgc cactcgtcgg ccagcaagtc
     1501 ataggtgcca aaattctgga gccggccact gaccggcagg agccagtgct cgcggcccgc
     1561 ttcccggtat ccttgcttgc gcagcgccgc cgccgagcgg gtattgggga agaagacata
     1621 cgactgcagc gaatgcatcc ccagcgtgtt gaacgcgtag tcgaagagca ggtgcttggc
     1681 ttcggatccg tacccgccac cgcggtactc cggccggatg atctcgctct ccgactcggc
     1741 acaccggttc tggtagtcga tgccgtcgat gccgaccgca ccgatcatct tgtcgttctc
     1801 gcgtaggcag accgcaaagc gcacccagtc ctgcggctct tccttctgaa gaccctcaaa
     1861 ccatttctga accgatgtct ccgtgtagag cggtcggcca ttactccaga atggctcggt
     1921 ctcccggcgc gaccatttga cgaccagttt cgcgtcggat ttctggagtg gccggaggta
     1981 gacgcgcgcg ccgatccgca tggcgttcgg cgggagcgtg ccctcgaccg tcaccggcgg
     2041 ggagaccgga cgaggctcgc cggttcccgt gcgctccagc tcaatgtcgg ccggatcacc
     2101 cagcgtctct atccactgct tgttcggata gtcgtactgg taggcatcga tataggacga
     2161 tccgcgccgg atcttctccc ggaagcggcc gcattcacgc gcaccgatcc gtgtcagccc
     2221 ctcgcgaatg atcggcttgt ctgcgggaat gtcgtacacg taggcgatga ctcgatgctg
     2281 ctcgtcgatg acccagggga taaccaagcc gagcgcctcg acgatccagc gatctccctg
     2341 ttcgccgaag aggcggtccg catagggtgt cagccacacg gcgtagcccc atcgttcgac
     2401 ctttaccgat ccgaccggcc ggtcgtccgc cttccggagg atcacatggt gcgtcgtgtt
     2461 gtgctcctcc ttcatctcct ccttgatcca cttcttgatg cggtcaacgg acttggggat
     2521 gacgctcccg aaccacgaca tgccgtaatc ggcgtcctct tcctcaaccg ggcgaacgta
     2581 gatcgattcg ccgacgagga aggcttggcc cacgtggccg gtgccggggg ccggtgtttc
     2641 ctgctttgac tctgtctctg ttgcggtagc tgacatggtt tattcctgtt catgcccaca
     2701 gggaccagcg atgcacgctg cctgtcggac gcaatgcacc cgtagcgagc caccggatcc
     2761 tggcggatgg cacgaaggcc atcacccgtt cctgcgggat gagatgttcg atcgatcgaa
     2821 tgtggtccgc tcggcggtga ggtcaccgac cgtcggatga gttacgcgag cgccatgacg
     2881 ccggcgtggg tatctgtgtt cacgagttga agcatcattg ccccacctcc tttcccaaca
     2941 agccctggac cgatgcatcg gggagatgac atcgggggcg atgagccgga gaggtccggc
     3001 gtcattgagg tccccacatg ccgcgagcga tgcggcagag ccgcgcgttc cagttctccg
     3061 aacggcgtgc tgcgcccaga atacacgatg ggatggcgat tgtgg
//
LOCUS       DASYWM010000167        16213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_25148, whole genome shotgun sequence.
ACCESSION   DASYWM010000167 DASYWM010000000
VERSION     DASYWM010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 16213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16213
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25148"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VGR29_06860"
     CDS             <1..634
                     /locus_tag="VGR29_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV2073351.1"
                     /translation="VASGTWCEGEPRNPDPRGVGRVNNAVLVLNQNYEPLNVCNVRRA
                     LVLVLGGKAEILEQNAEVVTTARAVYVVPSVIRLHYFIRRPRPRVKLSRREIFIRDNH
                     TCQYCGRRGGELTVDHVIPRSRGGLHVWENVVSACRPCNHRKGGKSVAEAHMDLRMQP
                     HEPRAGTYYTVERRLDLSQRDDWHKFLPELKPSTKWASRHSTNGSSSIPA"
     gene            768..2078
                     /gene="serS"
                     /locus_tag="VGR29_06865"
     CDS             768..2078
                     /gene="serS"
                     /locus_tag="VGR29_06865"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV2073352.1"
                     /translation="MIDIRIIRENPEEFRRRIATKGVDAPVDEILQKDEQRRALITEV
                     ERIKAERNEGSKQVARTRDTAERNALIAEMKGLGDKISNLDDQVRQVDEALNELLLGI
                     PNLPDEDVPVGPDETANVVTSEGGPEREFSFTPRPHWELAEELGIIDFERGVKVAGTR
                     GYVLRGDGAKLQRALIQWMLDVHTTRHGYSEIEPPLQVLSEMLVGTGNLPKFADTLFH
                     DAEEDKWLIPTAEVPITNLYRDEILPGEILPIYHVAATPCFRREQISAGRDVRGIKRV
                     YQFHKVEMVKFVHPDRSDEELKRLMSEAIYVVDQLGLNYHVLDLSTGDTTFGSAHTFD
                     IETWAPGSNEWLEISSCSNFRDFQARRANLKFRPEGGGRPQFLNTLNGSGLALPRVMI
                     AIIENYQREDGTIQIPEVLRPYFDGRDTIGKQPAFGPSAHIADV"
     gene            complement(2149..2235)
                     /locus_tag="VGR29_06870"
     tRNA            complement(2149..2235)
                     /locus_tag="VGR29_06870"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2199..2201),aa:Ser,seq:tga)
     gene            2348..2437
                     /locus_tag="VGR29_06875"
     tRNA            2348..2437
                     /locus_tag="VGR29_06875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2382..2384,aa:Ser,seq:gct)
     gene            2518..2593
                     /locus_tag="VGR29_06880"
     tRNA            2518..2593
                     /locus_tag="VGR29_06880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2551..2553,aa:Arg,seq:gcg)
     gene            2655..3683
                     /locus_tag="VGR29_06885"
     CDS             2655..3683
                     /locus_tag="VGR29_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HEV2073353.1"
                     /translation="MTRAAVKPKPLAPGDTIGIISPSWFGGPAYIPRARRGIAMLESL
                     GYKVRIAEHAFNSHGSVSDSAENRVLDLHSMFADDAVSAIFCTIGGDHSCHLLPLLDW
                     DLIASHPKIFLGFSDITVLNVAIRTQTGLVTFNGPTLLTDWAEYPTMPEVSKDSALRL
                     LTQPVPFGALPSAPEWTDEFLNWETGENTARRRKHHRSEGWRWIRTGKATGTLIGGCL
                     ESLQHLRGTRFWPDMGGAILFIETSEECRSPDAADAILMDYQNMGVFDEIAGLMVARP
                     YGMDPAATERFGCVIQERTEAFAFPVVGNLDIGHTSPLLTLPLGVRARIDGAAGTVEI
                     IESAVTEP"
     gene            3738..3824
                     /locus_tag="VGR29_06890"
     tRNA            3738..3824
                     /locus_tag="VGR29_06890"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3772..3774,aa:Ser,seq:cga)
     gene            3857..3945
                     /locus_tag="VGR29_06895"
     tRNA            3857..3945
                     /locus_tag="VGR29_06895"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3893..3895,aa:Ser,seq:gga)
     gene            complement(3910..4902)
                     /locus_tag="VGR29_06900"
     CDS             complement(3910..4902)
                     /locus_tag="VGR29_06900"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEV2073354.1"
                     /translation="MSGRVLVAGAINTDLVATVHRAPEAGETITGRSFAIHSGGKGAN
                     QAVAVARSRASAVMLGGVGRDDFGSARKADLAADQIDTSWIATIDYASSGVALITVES
                     SGENRIAYIPGATAKVSPDHCADTLNAIEPSLILSTNELSPECHRVLFTWARGRDVRV
                     IFNAAPYSDDVVELLPLIDVLIVNEGEASALQGGAPDGMSPQELAAGLRSIGATDVVI
                     TLGAGGSFAVSDDEPLTVPGYRVDVVDTTGAGDTFCGALASRMLEGATLPEAVRYANA
                     AGALATTRSGAQPSIPGREEIEKLMEQGKKLPWGSPQGGHGLRRKGRDSNPREV"
     gene            complement(4936..7530)
                     /locus_tag="VGR29_06905"
     CDS             complement(4936..7530)
                     /locus_tag="VGR29_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing transglutaminase
                     family protein"
                     /protein_id="HEV2073355.1"
                     /translation="MQEPSRSAQSSRLSLWFPEGAIVPVLLLVLIAIASFSVQFGGWS
                     SLVIPVGVLGVVAGVFGLVIAKARVADSIAHMGGLVIGVGVVFTLLLFEANSLGEGWR
                     DRVRPLGTFLIDWYLGRRSSAEHEDLLISMLMGLIVWLVGYLSAWVLFRRGWLMAAVL
                     LPGLLATVNLGYAENARAWLLGAMLAVAIPLGARYHLYQRQQIWTNYRMPSPLGLGRR
                     IVLTSMLAAVLITGLSWQAPSTWSQSNVQPFFEKLTERVTATQDRASDWLDHAAATGE
                     RVQDAGSYTAFDDAFSVGGPLDLSDREEILVRADVAQAPYLTAHSYDVYTGQGWASGI
                     DDSFARTGPDGERYSPELLFREGQSVVLSPNVTTDRVAQTIGVTPLGGESGVIFSIDT
                     YQSASIPAVVRMSWRQLDDEAFAVSAESLTSLPPDIQLISSYLTGAQLTGPLGEHGPQ
                     AVDPEQQARIDAELESLRGRMVSVRWEADAEGGVQTLYVSGQLPVYDDVEAAFPRNAA
                     DSGVSGSYQVTGLTSAADDQDLSNAGTSYPAWVTDRYLQLGDTVTPRTIELALDIAGG
                     AANPYEQAVLVEQWLRSNIAYDDRVAAPPDDADVVDYTLFELGRGYCEHFSSSMTVMM
                     RALGVPARTVVGYYPGEYDEARGGFLYRQLNAHAWTEVFFPAYGWIPFEPTANRPLSE
                     REMQESEAAASPEATEVLPESTPADEQVQSESTPAVEPPTLENLGPPMMTTVDEDGGV
                     PDWVLPVGAGSVMLAVVAGGAWLRWHWKLRGLPQSSALFSKLVRVGRLGGVKPSPTMT
                     PREYAANFSESLPLAGSSARRIVQVYELDQYGSAEVDAGRLAAAGEAWRQVRSQVPRL
                     LLRRRRQG"
     gene            complement(7573..8814)
                     /locus_tag="VGR29_06910"
     CDS             complement(7573..8814)
                     /locus_tag="VGR29_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV2073356.1"
                     /translation="MNAFKLVLLIAVLVAIAIVYNWSMLDNLIIALVALLVVAWLWSR
                     GSLNRLGLRRTLTSDRIRAGEVIEEELELTNHSWMPHLWIEVQDHSTLPDHHAGSVVS
                     VGGRATSTWRSQTRVLRRGVYRLGPFTLHGGDPFGIFEKRRLIPVSHELVVYPAPIDV
                     SSIPLPVAGMSGGTVRQGNIALSSPTIAGVREYAPGDPLNHVSWSATARRGVVMVKEF
                     EPDPTADLWLVLDLSERGQFNLEGRPTHGRPGALAESLLDTTAEYIVSIGGSLAEKAL
                     AEGRKVGIVINRSMPIRLDADNSQRQWFRVFEMLASATAFGNRSLAEALAVDARRFSS
                     AKGLVVVTSDPETDWAPAARALAERRVSVSAVVVDAGGEGDDSVTPLIEALAAARVGV
                     TRFPTHTAKAHHVEPFHVHAT"
     gene            complement(8821..9807)
                     /locus_tag="VGR29_06915"
     CDS             complement(8821..9807)
                     /locus_tag="VGR29_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV2073357.1"
                     /translation="MDSSTVRHLVETTTDNVERVIIGKRREVELVMVALLCRGHVLIE
                     DVPGVGKTVLTKAIARSIGSSFKRIQFTPDLLPSDVTGVNVFNQQRGTFEFRPGPVVA
                     QMVLADEINRATPKTQAAMLEAMEEAQISVDGVTHHLPSPFIVLATENPIDYEGTFPL
                     PEAQLDRFLLRLALGYPSRAGELEMLDRQHLRHPLEGLNQVISVDDLVRAQEAVKAVH
                     VSTAVREYIVALVESTRHHNDVFLGASPRGSIALFNATRAWAAIRGREFVLPDDVKLL
                     AEPTLAHRIIIGPPARMRGIDSRVVVHDLLNSIPVPQVGRQAMAPAQPLTRV"
     gene            9963..12755
                     /gene="polA"
                     /locus_tag="VGR29_06920"
     CDS             9963..12755
                     /gene="polA"
                     /locus_tag="VGR29_06920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV2073358.1"
                     /translation="MSGSTTLMAHAEISKDKGTIVLVDGYGLIFRAYFALPPTLTTGD
                     GEQTNAVYGFASMLLDVLRQRNPEYAVIALESGKTFRHELYADYKGTRSEMPEDLKSQ
                     ISRVRELIDALGIPVQQKEMYEADDVIGTLSRELADQGHQVIIITGDSDLLQLVDDNI
                     LVVLPGARRFGEFREFGPEAVFERYGFGPEYVPDYKALVGDTSDNIPGVPGIGDKTAK
                     KLIAQFGPLEEIIAHLEDVGPTRARNALAENIEQARESKALATIVRDVELDLDLSKAR
                     VHEFDREAITNLFRTLEFRSLLSKLPALVEEKTVVSQNGATEISEEPSEQTVVLTEEQ
                     LTAMIREIEKSGEIALDVETTGTDPFSADLVGIAIATSPTRSFYVPVGHKIGDQLSAD
                     LVRSALDPAIASDAVNVYTHHGKYDYHVLLRQGYTGHPITFDTMIAAYLLGETSIRLK
                     DLSFNRLGIQQTEITELIGSGKNQVTMAEVDFLKAAPYACADVECTFGLVAPLRADLE
                     GRKQLGLLYDIELPLATILIDMERIGVAIDHDALTEFSAELGQRIDAIDAEVDALAGR
                     PISVASNKQMAMLLFEELALPAGRKTKTGYSVDSEVLEGISDRHPIVPLVLEHRMLAK
                     LKSTYVDALPTYVHPVDGRVHTSYNQTVAATGRLSSNNPNLQNIPIRTEIGRRVRRAF
                     VADHRPEYRIYDDATLLAADYSQMELRILAHMSGDPALVTAFRQGEDIHRATAALVNG
                     ISPENVTSDQRRIAKTVNFGILYGMQAFGLSRDTGMPRGEAQEFIDRYWERLPKVKEF
                     FDKIIDFGRVHGYVETLSGRRRYLPDLNATNGARRQGAARMAMNMPIQGTQADIIKLA
                     MIQLDQAIRQRGLPASMVLQVHDELVLEIDKSRVEEIAGVVKGTMENAFALDVPVVAD
                     VRAGSNWEDMHAVDVG"
     gene            12878..13192
                     /locus_tag="VGR29_06925"
     CDS             12878..13192
                     /locus_tag="VGR29_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEV2073359.1"
                     /translation="MPTYAYRGKECPHEFEVYQSFSENALTECVECGKPVRRVIHPAG
                     IVFKGSGWYITDSRNGGKPADGENKTAKPEKTDSKSDSKTETTSNKSTKAPEPATACA
                     KD"
     gene            complement(13221..13349)
                     /locus_tag="VGR29_06930"
     CDS             complement(13221..13349)
                     /locus_tag="VGR29_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073360.1"
                     /translation="MESGCAGVDLSDTPEDSRYRRALLHTLTDKQPLRPGYLLRWA"
     gene            13445..14896
                     /gene="cysS"
                     /locus_tag="VGR29_06935"
     CDS             13445..14896
                     /gene="cysS"
                     /locus_tag="VGR29_06935"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV2073361.1"
                     /translation="MSQQPGSHVTTGLKVYNTLTREVEPFETLEPGKVSMYICGVTVY
                     DHAHIGHGMSSITFDVIRRYLMYLGYDVTYAQNFTDIDDKIINRANAEGIDPFDLTES
                     MIAAWDSEMSAFNILPATINPRATREMPQIIEMIQGLIDRGHGYEAGGDVYYKVRSFP
                     QYGKLSHRNVDELLIGARIAAGELKSDPLDFALWKAGKPGEPTWESPWSDGRPGWHIE
                     CSAMCMHHLGRQLDIHGGGADLIFPHHENEIAQSEAFTGIEPFARYWMHNGLLQFGSE
                     KMSKSLGNIVRLKDLVDRGRSMAFRLQVLSSHYRAPLTYTDEGLEAASNGLDRLRIAA
                     RPIDRSILVVADEDPNYHRDTELDRVVERAEETFHSAMLNDFDTPIAVASLFDLGRAV
                     NRARAAETLITSVLDAHTMLLKLANILGLDLTTPDTSDFIAAEPFIELLMQVRKDLRA
                     AKQFAIADQIRDELARLGVTLEDTKQGTTWKSS"
     gene            14856..15827
                     /gene="rlmB"
                     /locus_tag="VGR29_06940"
     CDS             14856..15827
                     /gene="rlmB"
                     /locus_tag="VGR29_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV2073362.1"
                     /translation="MKTPSKGQPGNRRKGTDSRGTGRPGGPDSGRREGTRPQRSGSGP
                     RQGRPPQRPVRDDATQNQRGSRPARPTKAAAAGIFIKDDLLYGRNAVRESLRAGRRTS
                     KRLFIAEGVREDERVEEIIQLAQEQEVLVERVPRMLLDDLAHGGNHQGIALDAAEYPY
                     VDLEDVIEREGTVVIMDHLNDPQNFGTLMRAADASGVAGIVLPQDRSVSVTPAVVNSS
                     AGAIEHLLVAQTPNLARALDKLEDSGRWIVGLDEDEESNSLFDVDIPTPVALVLGAEG
                     SGLSPIVRKRCQVVISIPMLGKVDSLNAATAGSIVLYDLLRRSTNQD"
BASE COUNT         3342 a         5014 c         4836 g         3021 t
ORIGIN      
        1 ggttgcatcc ggtacctggt gcgaaggtga accgcgcaat cctgatccga ggggcgtcgg
       61 cagggtgaat aacgcagttc tcgtcctgaa ccagaattac gagccgctca acgtctgtaa
      121 tgtgcgtcgc gcgctcgttc tcgtcctggg tggcaaagcc gaaatcctcg agcagaatgc
      181 ggaagtagtc acgactgccc gggcggtcta cgtcgtgccc tccgttatcc ggcttcacta
      241 cttcatccgc cgtccccgtc cccgggtcaa gctatcccgg cgggaaattt tcattcgcga
      301 caatcacacc tgccagtact gcggccggcg cggcggagag cttaccgtcg accacgtgat
      361 accacgctca cggggcggtc tccacgtctg ggagaacgtc gtctcggctt gtcggccatg
      421 caaccatcgc aagggcggaa agagtgttgc cgaggcccac atggacctca ggatgcaacc
      481 gcatgagcca cgggccggca catactacac cgtcgagcga cgtctcgacc tttcccagag
      541 ggacgactgg cacaagtttc tgcccgagct gaagccgtcc accaagtggg catcccgcca
      601 ctccaccaac ggatcctcta gtatccccgc gtgatctacg ctgaccaaca caggctcgct
      661 gcctcggtcc cgcgaagaga tcacgcgggg caccaccccc tgccagcgtg cctgaatctt
      721 gggcacgcca tcctccccgc gcgtatgaca ttgaaaggtt caggaacgtg atcgatatcc
      781 gaatcatccg ggagaacccg gaagaattca ggaggcgaat tgccaccaaa ggcgtcgatg
      841 caccggtgga cgaaattctc cagaaggacg agcagcggcg agctctcatc acagaggttg
      901 agcgaatcaa ggcagagcgc aatgagggct cgaagcaagt tgcgcgcacc agggacacgg
      961 ccgagcgtaa tgcactgatc gcagagatga agggactcgg tgacaagatc agcaatctgg
     1021 acgatcaggt acgccaggtc gacgaagcct tgaacgaact gctcctcggc attcccaacc
     1081 tcccggacga ggatgtcccg gtaggcccgg acgaaaccgc caacgttgtc accagcgaag
     1141 gtggaccgga gagggagttt tcattcactc cgaggccgca ctgggagctt gccgaggagt
     1201 tgggaattat cgactttgag cgaggtgtga aggtcgccgg tacacgcggc tatgttctgc
     1261 gtggcgacgg ggcgaagctg cagcgagcat tgatccagtg gatgctcgac gttcacacga
     1321 cccgtcatgg gtacagcgag attgagccgc cactgcaggt gctttccgag atgctcgtgg
     1381 gaaccggcaa tctgccaaag tttgccgaca cgctttttca cgatgccgag gaggacaaat
     1441 ggctgatccc caccgcggag gttccgatca cgaatctcta ccgggacgag atcctgcccg
     1501 gcgagatatt gcccatctat cacgtcgcgg caactccctg cttccggcgg gagcagatat
     1561 cggcgggacg cgatgttcgg ggcatcaagc gcgtgtacca gttccacaaa gtggagatgg
     1621 tcaagttcgt ccacccagac aggtcagacg aagagttgaa gcgcctgatg agcgaggcga
     1681 tttacgtcgt cgatcagctg ggcctcaact accacgtgct ggacctgtcc accggggaca
     1741 cgacgtttgg ttcagcacac accttcgata tcgaaacgtg ggcgcccggg tcgaacgaat
     1801 ggcttgagat ttcatcgtgc tcgaacttcc gcgacttcca ggctcggcgt gcgaacctga
     1861 agttcaggcc ggaaggcggc ggccgaccgc aattcctgaa cacgctgaat ggctccggtt
     1921 tggccctccc acgggtgatg attgcgatca tcgaaaatta ccagcgcgag gacgggacga
     1981 tccagattcc cgaggtgctc cgcccctact tcgacggccg cgacacgatc ggcaaacagc
     2041 cggcattcgg cccgtcagca catatagcgg acgtataacg aggctgcacc aggtggatgg
     2101 tcgaaacaga acgccccgct caataggacc ggggcgttct gtcgaaggcg gagagggtgg
     2161 gattcgaacc cacggtaggt ttgaccctac gacggttttc aagaccgctc cattcgtccg
     2221 ctctggcacc tctccggcgc ccagtttact tcatctgccc gtgatcttgc aggtgtcgtt
     2281 cggcgcggag agcacctcgt tcatgtcgac gccaaagccg gccatctgct agacttgggc
     2341 cgcaactggg gaggtggccg agcggttgaa ggcgctcgcc tgctaagcga gtatggaggt
     2401 aatgcctcca tcgagggttc gaatccctcc ctctccgccg gtcgcgtgta aagccagcct
     2461 gcaccgggtt cctccttgcg agcattcgtt cggccggact atactttgcc ccggtatgcg
     2521 cccgtagctc aggggataga gcgttgggtt gcggacccaa aggccggagg ttcgaatcct
     2581 cccgggcgta ccaatccagg agagccgctc atatcgagcg gcttttttgc accccatcat
     2641 ggagttcatc acggatgacc cgagcggcgg tcaaaccgaa acctctcgca cccggcgaca
     2701 cgatcggcat tatcagcccg tcgtggttcg gcggtcctgc ctatattccc cgggcgcgcc
     2761 ggggaatcgc gatgctcgag tctcttggct acaaggtcag gatcgctgaa cacgcgttca
     2821 acagccacgg ctctgtgtca gactccgcgg agaaccgggt cctcgacctg cactccatgt
     2881 ttgccgatga tgccgtctca gccatcttct gcacaatcgg tggtgatcac agctgccatc
     2941 ttcttcccct cctggactgg gatctcatcg cgagccatcc caagatcttt ctcggcttct
     3001 ccgatatcac cgtcctaaac gtcgcgattc gcacccagac tggcctcgtg accttcaacg
     3061 gccctacctt gctgaccgac tgggccgagt acccgacgat gccggaggtc agtaaggact
     3121 ccgcgcttcg actcctcacg cagccggtac ccttcggtgc gctgccgtcc gctccggagt
     3181 ggaccgatga gtttctcaac tgggaaacag gagagaatac ggcgcgccgc cgaaagcatc
     3241 accggagtga gggctggcgc tggatcagga ccggcaaggc gacaggcacg cttatcggcg
     3301 gttgcctgga gagcctgcag catcttcgcg ggactcgctt ctggcccgac atggggggag
     3361 ccattctctt cattgagacc tcggaggaat gccgctcgcc agacgccgcc gacgcgatcc
     3421 tgatggacta ccaaaatatg ggcgtcttcg acgagatcgc cggcctgatg gtagcacggc
     3481 catatggaat ggaccctgct gccacggagc gatttgggtg cgtcatccag gaacggacag
     3541 aggcgttcgc gttccccgtt gtgggtaacc tggacatcgg ccacacatct cctcttctga
     3601 ccctcccgct cggcgtacgg gcccggatcg atggtgctgc aggcacggtc gagattatcg
     3661 aatcagcggt gacagagccg tgatcgccca tggaaaacgc catgacatca cccgcccagt
     3721 ttggtacaat ctcgcgcgga gaggtgtccg agcggtttaa ggtgctgctc tcgaaaagca
     3781 gtgtggtgtc aaagccaccg cgggttcgaa tcccgccctc tccgctgcgg gacaccgatc
     3841 ttgcaggaaa aagcatggaa gggtcgcata gtctggccta gtgcgcacga ttggaaatcg
     3901 tgtagggggt taaacctctc gagggttcga atccctcccc ttccgccgca gaccatgacc
     3961 gccttgggga cttccccaag gcagtttttt gccctgctcc atcagcttct cgatctcttc
     4021 ccggccggga atcgacggtt gcgcgccgga gcgggtggtc gccagcgctc ccgcggcgtt
     4081 ggcataccgg actgcctccg gcaacgtcgc tccctccagc atccgggaag caagtgctcc
     4141 acagaacgtg tctccggcgc ccgttgtgtc gaccacgtcg acccggtaac ccggcacggt
     4201 caatggttca tcgtcgctta cggcaaaaga gccgcccgcc ccgagcgtaa tgaccacatc
     4261 ggttgctccg atcgacctga ggcccgctgc gagctcttga gggctcatcc catccggcgc
     4321 tccgccctgc agcgcggatg cctcgccctc gttcacgatc aggacatcga tgagaggaag
     4381 caattcgact acgtcatcgg agtagggagc ggcattgaag atcacccgca cgtcccgtcc
     4441 acgagcccag gtgaagagaa ccctgtgaca ttcgggagag agctcgttgg tcgaaagtat
     4501 cagcgagggt tcaatggcat taagtgtatc ggcgcagtgg tcgggagaga ccttcgcagt
     4561 cgctcccggg atatacgcaa tgcgattttc tccggatgac tcgacggtga tcagcgctac
     4621 tccggacgat gcatagtcga tcgtcgcgat ccacgaggtg tcgatctggt cagcggccaa
     4681 atccgccttt cgggctgaac cgaaatcatc tcgaccgact cccccgagca tgacggcgga
     4741 tgcacgggat cgggctacgg caactgcctg gttcgcgccc ttgccgccgc tatggatagc
     4801 gaatgaacga ccggtgatcg tctcccccgc ctcgggagca cgatggacgg tcgcgacgag
     4861 atccgtgttg atagcgccgg caacgagaac ccgacctgac atccgtactc tccgttttgt
     4921 ggggaagccc cgactctatc cttgtcgccg ccgccggagc agcaggcgtg gtacctggga
     4981 tcgtacctgg cgccatgcct cacctgcagc agccagccgt cctgcatcta cttcagccga
     5041 gccatactgg tcgagctcgt agacttggac gatccgccgc gcggaagacc cggcgagggg
     5101 aagtgactcg gagaagtttg ccgcatattc ccgtggtgtc atggttgggg acggctttac
     5161 tccacccaat cggccaaccc ggaccagctt cgagaagagc gcggagctct ggggcagacc
     5221 ccgaagcttc cagtgccatc tgagccacgc ccctcccgca acgaccgcga gcatcaccga
     5281 gccggctccg accggcagaa cccagtccgg cactcctccg tcctcgtcaa cggtcgtcat
     5341 catcggtgga cccaggtttt ctaaggtcgg cggctcgacg gctggcgtgc tctccgattg
     5401 gacctgttca tctgcggggg tgctttccgg aagaacttcc gtcgcctccg gcgacgctgc
     5461 tgcttccgac tcctgcatct cacgctcgga aaggggccgg ttggctgttg gttcgaacgg
     5521 aatccagccg tatgccggga agaaaacttc cgtccaggca tgggcgttca gctggcggta
     5581 gaggaatcct cccctcgcct cgtcatattc accggggtaa tagccgacga cagtccgggc
     5641 cggcacaccg agcgcccgca tcatcaccgt catggaagaa gagaagtgct cgcagtaccc
     5701 gcggccgagc tcgaaaagcg tgtagtcgac gacatccgca tcatctggtg gagcggccac
     5761 gcgatcgtca taggcgatgt tggatcgcag ccattgttcg accaaaaccg cctgttcgta
     5821 tgggtttgcc gccccgcctg cgatgtccag cgcgagctcg attgtacggg gcgtcaccgt
     5881 gtcgcccagc tgaaggtagc gatctgtgac ccaggccgga taactggtgc ctgcatttga
     5941 caggtcctgg tcgtcggctg cgctcgtgag accggtcacc tggtatgagc cggaaacacc
     6001 agaatcggcg gcgttacgcg gaaaggccgc ttccacatcg tcataaacgg gaagctggcc
     6061 agagacatag agcgtctgaa ccccgccctc ggcatcggct tcccatcgga ccgaaaccat
     6121 gcgtccgcgc aggctctcca actcggcgtc gatccttgct tgctgctcgg ggtcgactgc
     6181 ctgcggtccg tgctccccga gcggtcccgt gagctgtgca ccagtcaggt aactgctgat
     6241 gagttgaata tccggcggta gggacgtcag cgactcggcg ctgacagcaa acgcttcatc
     6301 atccagctgc cgccaggaca tccggacaac ggcaggaatc gaagcggact ggtaggtatc
     6361 gattgaaaag ataacgcccg attcaccacc aaggggcgta accccaatgg tctgggcaac
     6421 acgatcggtt gtcacattcg gtgagaggac aacactctgc ccctcacgaa acagcaactc
     6481 cggtgagtag cgctccccgt caggaccggt tcgcgcaaaa ctgtcgtcga taccggaggc
     6541 ccatccctgc cccgtataca cgtcatagct gtgagccgtg agatagggcg cctgggcaac
     6601 atcggctctg acgaggattt cctctcggtc actcaggtcg agcggaccgc cgaccgagaa
     6661 agcatcatcg aacgcggtat atgagccagc atcctggacg cgttcacctg ttgcagccgc
     6721 atggtcgagc caatctgagg cgcgatcctg cgtcgccgtc actcgttcgg tgagtttttc
     6781 gaagaagggc tggacgttgc tttgcgacca tgtggatggt gcctgccagc tcaaaccggt
     6841 gataagcacg gcagcgagca tgctggtcag cacgatccgt ctgccgagcc ccaaagggga
     6901 cggcattcgg taattcgtcc agatctgctg acgctgatag aggtgatacc gcgctccaag
     6961 aggaatcgca acagccagca tggctccaag caaccatgcg cgagcgttct cggcatagcc
     7021 caggttgacg gtagcgagga gtcccggcag aaggaccgcg gccatcagcc agcctctcct
     7081 gaacaggacc cacgcggaca ggtaaccgac gagccagacg ataagaccca tcagcatcga
     7141 gatcaggaga tcttcgtgct ctgccgacga gcgccggccc aaataccagt cgatgaggaa
     7201 ggtacctagc ggtcggaccc tgtcccgcca tccctccccg agcgaattcg cctcgaagag
     7261 gagtagcgtg aacacgactc ctacaccaat caccaggcca cccatatggg cgatggaatc
     7321 agccaccctc gccttcgcaa tgaccaaacc aaacacaccg gcgactaccc ccaggacccc
     7381 caccggtata acgaggctgg accatccgcc aaactggacg ctgaaggacg cgatagcaat
     7441 aagtacgagc aacagaaccg gcacgatcgc gccctcgggg aaccagagtg agaggcgtga
     7501 agattgagcg gaacgcgagg gctcttgcat attgattgca gtggtagggc ttgatcggtt
     7561 catcaattgt atctacgtcg catgcacgtg aaacggctcc acgtggtgag ccttggccgt
     7621 atgcgtcggg aagcgggtaa cgccaactcg ggctgcggcc aacgcctcga taagcggagt
     7681 gacgctgtca tccccttctc ccccagcatc gacaacaacg gcagaaacgc tgacccggcg
     7741 ctcggcaagc gctcgcgcag ctggtgccca gtcagtttcg gggtccgatg tcaccaccac
     7801 caaacctttg gcgctcgaaa atcggcgtgc atccacggca agcgcctcgg cgagggagcg
     7861 atttccgaac gccgtcgccg acgcaagcat ctcgaagaca cggaaccatt gtcgctggga
     7921 attatcggca tccagccgga tcggcattga gcggttgatg acaattccga cctttctgcc
     7981 ttcggcgagt gccttttccg cgagagatcc gcctatggac acgatgtact cagccgtggt
     8041 gtcaagaagt gattcagcca gcgctcccgg gcgcccgtgc gtcggccgtc cctcgagatt
     8101 gaattgtcct cgctcgctga ggtcaaggac aagccatagg tcagccgtcg ggtcaggctc
     8161 gaactccttt accatcacga cgccacggcg tgccgttgcg gaccacgaga cgtgattcag
     8221 gggatcgcca ggggcgtatt cgcgaacacc ggcgatcgtt ggcgaggaaa gcgcaatatt
     8281 cccctggcgg accgttcctc ctgacatgcc ggctacaggc agcgggatcg aggagacatc
     8341 gatcggcgcc ggatagacga cgagttcgtg ggacaccgga atcaaccgcc gcttctcgaa
     8401 gatgccaaac ggatctcctc catggagagt gaatggaccc agtcggtaga cgccccggcg
     8461 gagcactctc gtttggcttc gccatgtgct tgtagccctt ccccccacac tcacaaccga
     8521 ccccgcgtga tggtcgggca gcgtggaatg gtcctggact tcgatccaga ggtgcggcat
     8581 ccacgaatga ttcgtcagct cgagctcttc ttcgatgacc tctcctgccc gaatcctgtc
     8641 ggaagtcaat gtccgtcgca gcccgaggcg gttcaggctg ccacggctcc agagccaggc
     8701 gacaaccagc agggccacca gcgcgatgat gaggttgtcg agcatcgacc agttgtagac
     8761 gatcgcgatg gcgaccagca cggcgatcag gagtacgagc ttaaaggcgt tcaaggaggg
     8821 tcagactctg gtgagcggct gcgcgggagc catcgcctgg cgtccgacct gcgggaccgg
     8881 aatgctgttc aggaggtcgt gaacaaccac ccggctatcg atgcctcgca tgcgtgccgg
     8941 cggcccaatg atgatccgat gggccaacgt gggctccgcc agcaatttga catcgtcagg
     9001 cagcacgaac tcgcggccgc ggatcgccgc ccacgctcgt gtcgcgttga acagcgcgat
     9061 cgatccgcga ggacttgccc caaggaacac atcgttatgg tgtcgggtcg actcaacgag
     9121 cgcgacgata tattcacgga ccgctgtgga aacgtgaacc gccttgaccg cttcctgcgc
     9181 cctcaccagg tcgtcaacgg agatcacttg gttcaggccc tcaagggggt ggcgcaggtg
     9241 ctggcgatcc agcatctcca gttcccccgc gcggctcgga tacccaagcg cgagccggag
     9301 aaggaatcga tccagttggg cctcgggaag gggaaacgtc ccttcataat cgatagggtt
     9361 ttcggttgcg aggacgataa agggcgacgg aaggtggtgg gtcacgccat cgaccgagat
     9421 ctgggcctct tccatcgctt ccagcatcgc ggcctgggtc ttgggcgtcg ctcggttgat
     9481 ctcatcggcg agcaccatct gggcaaccac cggtccgggc cgaaactcga acgtgccgcg
     9541 ctgctggttg aagacgttga cccccgtcac atcgctcggc agcaggtctg gagtgaactg
     9601 gatgcgcttg aagctgctgc caatactccg ggcaatcgct ttggtcagca ccgtcttgcc
     9661 aacgcccggc acgtcctcga ttagcacatg tccgcggcac agcagggcaa ccatcaccag
     9721 ctccacctcg cggcgtttgc cgatgatcac acgctctaca ttgtcggtcg tcgtctctac
     9781 cagatggcga acggtcgaag aatccatgaa cagggaaggc tcctggcaga aacgggcgag
     9841 attgacgcat ggtggcgggg atgcctccgc gcagtgtccc gcaaagtata ccggagtcct
     9901 ttcctctccc cggccaccga tcaaaccatc gaccctatac ttcaagcctg acggtttcgc
     9961 acatgtctgg gagcacgaca ctgatggctc atgccgagat ttcgaaagac aaggggacga
    10021 ttgttctggt ggacgggtac ggcttgatct tccgtgccta cttcgccctt cctcccacat
    10081 tgacgaccgg ggatggagag cagacgaacg cggtgtacgg gttcgcatcg atgctgctgg
    10141 acgttctccg ccagcggaac cccgagtatg ccgttatcgc ccttgaaagc ggcaagacct
    10201 ttcgccatga gctctacgcc gactacaagg gcacccgctc cgagatgccc gaggacctga
    10261 agtcacaaat cagccgcgtc cgggagttga tcgacgccct cggcatcccc gtccagcaga
    10321 aggagatgta cgaggcggac gacgtcatcg gaactctctc ccgcgaactg gcggaccagg
    10381 gtcaccaggt catcatcatc acgggcgatt ccgaccttct gcaacttgtc gacgacaata
    10441 tcctcgtggt cctgcccggt gcgcgtcggt tcggggagtt ccgcgaattc gggcctgaag
    10501 cggtctttga gcggtacggg ttcgggcccg aatatgtccc ggattataag gcgctcgtcg
    10561 gagatacgtc cgacaacatc cccggtgttc ctggcatcgg tgacaagacc gccaagaagc
    10621 tgatcgccca gtttggaccc cttgaggaga tcatcgcgca tctggaagat gttggtccga
    10681 cccgggcgcg caacgcgctg gctgagaata tcgagcaggc cagggagagc aaggcgcttg
    10741 caaccatcgt gcgagacgtc gaacttgatc ttgacctgag caaggctcgt gtacacgagt
    10801 tcgaccgcga agccatcacg aacctcttcc ggaccctcga gttccgctcg ctcctctcca
    10861 agctcccggc gctggtcgag gaaaagactg ttgtctccca gaatggcgcg acggagatct
    10921 cagaggagcc ctcggaacag acggtcgtcc tgacagaaga gcagctcacc gcgatgatcc
    10981 gggagattga gaagagcggt gaaatcgccc ttgatgtcga gacgacaggc accgaccctt
    11041 tcagcgctga tctcgtcggc atcgccatcg caacgtcacc cacccgatcg ttctacgtac
    11101 cggtgggcca caaaatcggc gaccagctca gcgccgatct ggtccgctcc gcgcttgacc
    11161 cggcgatcgc cagcgatgcg gtgaacgtct atacccatca cggcaagtac gactaccacg
    11221 tgttgctacg ccaggggtac accgggcatc cgatcacctt cgacacgatg atcgcggcct
    11281 acttgctcgg cgaaacgtcc atccggctca aggatctctc cttcaatcgc cttgggatcc
    11341 aacagacgga gatcaccgag ctcatcggtt ccggcaagaa ccaggtgacg atggccgaag
    11401 tcgacttctt gaaggcggcc ccctatgcct gcgcggacgt ggagtgcacc tttggactcg
    11461 tggctcctct ccgcgcagat ctggaaggac gaaaacagtt aggtctgctc tatgacatcg
    11521 agttgcccct ggccaccatc ctcatcgaca tggagcgcat tggagtcgca atagaccacg
    11581 acgcgctcac agaattctcc gccgagctcg gtcagaggat tgacgccatc gacgccgagg
    11641 tcgacgcgct cgcaggccgg ccgatcagcg tcgcatcgaa caagcagatg gcgatgctcc
    11701 tctttgaaga actggcgctt ccagcgggcc ggaagacgaa gaccggatat tctgtcgatt
    11761 cagaggttct ggaggggatc agcgaccggc accccatcgt ccccctcgtc ctcgagcacc
    11821 ggatgctggc caagctcaag tccacctatg tcgacgcgct tcccacgtat gtccatcctg
    11881 tggacggccg ggtgcatacg tcctacaacc agaccgtggc agcgacggga cggctctcct
    11941 ccaacaatcc aaacctgcaa aacatcccga tccggaccga gatcggccgc cgcgtccggc
    12001 gcgcctttgt tgccgaccat cgtcccgagt accgcatcta cgatgacgcg actctccttg
    12061 ccgccgatta ctcccagatg gagctgagga tcctcgccca catgtccgga gatcctgcgc
    12121 tggttacggc attccgccag ggcgaggaca ttcatcgagc cacagcggcg ctggtcaacg
    12181 gaatcagtcc tgagaacgtg acgtccgacc agcgccgaat agcaaagacg gtcaacttcg
    12241 gcatcctcta cggaatgcag gcctttggtc tctctcgcga tactggcatg ccacgcggtg
    12301 aggcgcagga gttcatcgat cgttactggg agcgtcttcc aaaggtgaag gagttcttcg
    12361 acaagatcat cgattttgga agggttcacg ggtacgtaga gacactttcc ggacgtcgtc
    12421 gctacctgcc tgatctcaac gcgaccaacg gcgcccgtcg gcagggcgct gctcgcatgg
    12481 cgatgaatat gccgatccag gggacgcagg ccgacattat caagttggcg atgattcagc
    12541 tcgaccaggc gatccgacaa cgcggccttc ccgcttcaat ggttctccag gtccatgacg
    12601 agctggtgct ggagattgac aaatcccggg tggaggagat cgcaggggtt gtgaaaggca
    12661 ccatggagaa cgcgttcgcg ctcgatgttc cggtggtggc tgatgtgcgg gcaggaagca
    12721 attgggagga catgcacgcc gttgatgtag ggtgaagaca tcgatccctc cctgcggctc
    12781 ttttgcgcga tttcgggaaa gaaggataca ctgctaaccg tggccctcgg ggccgtctcg
    12841 tcttcccttt cattggtttc agaggagttg agggtagatg cccacgtacg cctaccgcgg
    12901 caaggaatgc ccgcatgagt ttgaggtgta tcagtcgttc tcggaaaacg cgctgacaga
    12961 gtgcgtagag tgcggcaagc cggttcgtcg ggtcattcac cctgccggga tcgtcttcaa
    13021 ggggtcgggc tggtacataa ccgacagccg caacggtgga aaacctgccg acggcgagaa
    13081 caagacagcc aaaccggaga agacggactc gaaatcggac tccaaaacgg agacgacctc
    13141 gaacaagagc accaaagcac ccgagcccgc tactgcctgc gcaaaggatt agcaattccc
    13201 gatgttcgcc ctgtgcatct ctacgcccat cgaagcagat accccggccg aagtggctgc
    13261 ttgtccgtca gggtgtggag caatgcgcgc cgatagcgac tgtcctcagg ggtgtcgctg
    13321 agatcaacgc cagcgcaccc cgactccatc gtgtcctccg gtccgtgctt cgggactttc
    13381 ccaaaggtcc catgtcccgg tggacacctg atcacacccc tcccagcgtc gatggagaca
    13441 gtccatgagt cagcaacccg gaagccatgt caccaccggt ctcaaggtct acaacacact
    13501 cactcgcgaa gtagagccct tcgagacact tgagcccggt aaggtcagta tgtatatctg
    13561 cggtgtgacg gtatatgacc acgcgcacat cggtcacggt atgtcgtcca tcaccttcga
    13621 tgttatccgc cggtatctga tgtaccttgg ctatgacgtc acgtacgccc aaaactttac
    13681 cgatatcgac gacaaaatta tcaaccgggc caatgcggag ggcatcgacc cgttcgatct
    13741 taccgaatcg atgatcgctg cctgggacag cgagatgtcg gcgttcaaca tccttccggc
    13801 gacgatcaac ccgcgcgcaa cccgggagat gccgcagatc atcgagatga tccagggctt
    13861 gattgacagg ggacacgggt acgaagcagg cggcgatgtc tactacaagg tccgctcatt
    13921 cccccagtac gggaaactat cgcatcggaa cgtcgatgag ctgctgatcg gcgcccgcat
    13981 tgccgctggt gagctcaaga gcgaccctct tgatttcgcg ctctggaagg ccggcaaacc
    14041 gggtgagccc acttgggaaa gtccatggag cgacggcagg ccggggtggc acatcgagtg
    14101 ctcggcgatg tgcatgcatc atctcggacg gcagctggac attcacggcg gtggggccga
    14161 cctgatcttc ccccaccacg agaacgagat cgcccagtcc gaagcgttca ccggtattga
    14221 gccattcgcg cgatactgga tgcataacgg ccttctccag ttcggatccg agaagatgtc
    14281 gaagtcgctc ggcaacatcg tgcgcctcaa ggacctggtg gatcgcggcc gatctatggc
    14341 cttcaggctg caggtccttt catctcacta cagggcgccg ttaacgtata cggatgaggg
    14401 cctggaggcg gccagcaacg ggctggaccg tcttcgcatt gccgcccgtc caattgatcg
    14461 gtccatccta gtagtggctg acgaggaccc gaactatcac cgagatacag aacttgatcg
    14521 agtggttgag agggccgagg aaacgttcca cagtgcgatg ttgaacgact tcgatacgcc
    14581 catcgcggtc gcctcgctct tcgatctagg acgagccgtg aatcgtgcgc gtgcagcgga
    14641 gacgctaata acgtccgtac tagacgcaca cacgatgctg ctaaaactcg ccaatatcct
    14701 tggcctagat ctgactaccc cggatacaag cgacttcatc gctgccgagc ccttcatcga
    14761 actactgatg caggtccgca aggatttgcg ggcggcaaag caattcgcca tcgccgacca
    14821 gatccgggac gaactggcgc ggctgggagt aacacttgaa gacaccaagc aagggacaac
    14881 ctggaaatcg tcgtaaaggc acggatagcc gaggcactgg ccgtccagga gggccggata
    14941 gcgggcggcg tgagggaacc cgtccacagc gctcgggctc tggacctcga cagggcaggc
    15001 cgccgcagcg accggttcga gacgacgcga cccagaatca gcgagggtca cgtcccgccc
    15061 gcccgaccaa ggcagctgcc gccgggatct tcatcaagga cgatctgctg tacgggcgca
    15121 acgccgtacg cgagtcgtta cgcgcagggc gccgcacctc gaaacggctc ttcattgctg
    15181 agggtgtccg ggaagacgag cgcgtcgagg aaatcataca actggcgcaa gaacaggagg
    15241 tcctggtcga gcgcgtcccc cgcatgctgt tggacgacct cgcgcatggc ggaaatcacc
    15301 aggggatcgc gctggacgcc gccgagtatc cctatgtcga tcttgaggat gtcatcgagc
    15361 gcgagggcac cgtggtgatc atggatcatc tcaacgatcc gcagaacttc gggaccttga
    15421 tgcgggctgc cgatgcgtca ggagttgcgg gtatcgtcct cccccaggac cgctctgtga
    15481 gcgttacgcc ggcagtggtg aattcctcgg cgggcgcgat cgagcacctc ctggtcgcac
    15541 aaacgccgaa cctcgcccgc gcgctcgaca agctggaaga ttcgggccgc tggattgttg
    15601 gcctcgatga ggacgaagaa tcaaacagcc tcttcgatgt cgatattccg actcccgttg
    15661 cgctggtgct cggcgcggag ggcagcggtc tttcccccat cgtccgcaag cgctgccagg
    15721 tggttatctc gatcccgatg ctaggaaagg tcgactcact caacgccgcg acagcggggt
    15781 caattgtcct ctacgacctc ttgcgacgga gcacgaacca ggattagcgg tgtgtcgggg
    15841 cttcatttgc gattcccatg gagttgtgcc cgacgttcac ccggtggccg actcgcttgc
    15901 ggtctcccgt gtgagcggcc cgttcacatg ggagcacatc tgtaaacagg ctaccgtgac
    15961 gcgtctgagc agacgcgtcg cggtagcctg tttacagatg tgctcccatg tgaacgggcc
    16021 gctcagacgg gagaccgcaa gcgagtcggc caccgggtga acgtccggca caactccatg
    16081 ggaatcgcaa atgaagcccc gacaccccgc taatcctggt tcgtgctccg tcgcaagagg
    16141 tcgtagagga caattgaccc cgctgtcgcg gcgttgagcg agtcgacctt tccaagcatc
    16201 gggatcgaga tga
//
LOCUS       DASYWM010000168        10157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_25162, whole genome shotgun sequence.
ACCESSION   DASYWM010000168 DASYWM010000000
VERSION     DASYWM010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10157
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25162"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /gene="plsX"
                     /locus_tag="VGR29_06945"
     CDS             <1..1046
                     /gene="plsX"
                     /locus_tag="VGR29_06945"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV2073363.1"
                     /translation="GKGISMRIAIDAMGGDHGPSVVVPGVLAGARRHGISLLLVGRKQ
                     ELENSLAGDDTSGIDVEIVDATQTIDMDDHPAQAVRRKTDSSINVALRLLKESRANAM
                     LSAGNSGAVMAASLMILGRIKGIERPAITSGIPNAKGTYSLIVDLGAVTDPKPNNMVQ
                     FAVMGQVYAQAVMGVLNPTIGLVSNGEEASKGNALVQQVHPLLRATEGLNFVGNVEGK
                     DIASGTVDVFVMDGFTGNVVLKTIEGTASMLMHLVREEITSTLPRKLAALVLKPAFRQ
                     VSGKLDYAAIGGAPLLGVDGTVVICHGRSSEMAFENAVGVCKRAVEHDLSGEIYRRVA
                     ATHEFVSEAHAGT"
     gene            complement(1123..1395)
                     /locus_tag="VGR29_06950"
     CDS             complement(1123..1395)
                     /locus_tag="VGR29_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV2073364.1"
                     /translation="MRKQDLVRAVAEATQQNEGSSSKAVNAVFSAIEDALSSGDEVTI
                     SGFGSFKVVERAARQGRNPQTGEEMTIKARKSPVFRPGTQLKRAVE"
     gene            complement(1537..2352)
                     /locus_tag="VGR29_06955"
     CDS             complement(1537..2352)
                     /locus_tag="VGR29_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV2073365.1"
                     /translation="MSCIHSTGDVIASNSNPGIKLIRSLQRRKIRLQERAFIVEGVRA
                     VRDVIHAEMIPRVVYLRDDFDLDLVPHLLDDVPLRRVIAPVFDSLTDVPHPQGILAVV
                     PMPAESNLPEASSGRAPLLLIVDAVRDPGNLGTLLRSAAGAGADHVVITPETVDPYHP
                     RAVRAAMGAHVRVALSHRDWDELATSLKRYDVVALADASGETEYDRLSWLRSAALIVG
                     SEAFGPTASARRCATTRVAIPLAGGVESLNAGVAGSLLLFEAARQRRANDALE"
     gene            complement(2368..2736)
                     /gene="rplT"
                     /locus_tag="VGR29_06960"
     CDS             complement(2368..2736)
                     /gene="rplT"
                     /locus_tag="VGR29_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV2073366.1"
                     /translation="MARVKRGVAAHRRHRSVLKQVKGHRGTRNRLYKRAHESLMHALQ
                     YAYRDRRNRKRAMRRLWIQRINAASRLNGMPYSHLVHGLTLAGVEVDRKILADLAVRD
                     EEGFAAVVQVAKSSLPSAQA"
     gene            complement(2776..2988)
                     /gene="rpmI"
                     /locus_tag="VGR29_06965"
     CDS             complement(2776..2988)
                     /gene="rpmI"
                     /locus_tag="VGR29_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV2073367.1"
                     /translation="MSKQGKLKTHKGAKRRFKVTATGKIMHAKGMKSHNRRKKSARVK
                     RQFDRMLVMNDASVKRVKRLIPNGIS"
     gene            complement(2972..3574)
                     /gene="infC"
                     /locus_tag="VGR29_06970"
     CDS             complement(2972..3574)
                     /gene="infC"
                     /locus_tag="VGR29_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV2073368.1"
                     /translation="MPRAPSYVFRRIAISRPVQTRVNERIRIREVRLIDDEGQQIGII
                     PTREALDMARERGLDLVEVAPNASPPVCRLMDYGKYKYEQSRKERDSRRSQHVIKVKE
                     VRIEPKIGGHDLETKGRQAAKFLDAGDKVKLTVLFRGRSITHPELGKDLLERLAEQLK
                     DHGVIEQSARMEGRTMTMHMAPSRQRAESSDREEQREQAG"
     gene            complement(3661..4617)
                     /gene="ribF"
                     /locus_tag="VGR29_06975"
     CDS             complement(3661..4617)
                     /gene="ribF"
                     /locus_tag="VGR29_06975"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEV2073369.1"
                     /translation="MAERVFASFSDVTASPTVATIGAFDGVHRGHQHLLRLAREGAKE
                     HESRLLVLTFEPLPIQVLRPETFAGRVLTKERRRGLLFRYGADLVVELPFTLEMSRMC
                     AEEFVAQLFAVGPVRQIWVGSDFALGHKRSGTPARLAEIAAPSGTTVRAVPRVDYKGQ
                     EVSSTRIRNLILQGRPDDASFILGHRFQVRGEVVRGAQVGRTIGFPTANVVPPEDLVP
                     LQDGIYATFARILDNDAIFEAMTYIGTRPAVNTGDRMIETHLFGFDGDLYGRTLETEF
                     VRRLRPDANFRSVDALKAQLARDEEHAREVLTSRRPGEVEDS"
     gene            complement(4623..5495)
                     /gene="truB"
                     /locus_tag="VGR29_06980"
     CDS             complement(4623..5495)
                     /gene="truB"
                     /locus_tag="VGR29_06980"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV2073370.1"
                     /translation="MIDKPAGWTSHQVVGNVRRILSERRVGHAGTLDPAAVGVLPIAV
                     GLATRTVEYLSGASKAYIADVTFGVTTDSADVDGVVTSVGDTSRLQEFDIVQVLESFF
                     GDQFQVPPAYSAIKVNGERLYELARRGESVELEPRPIRIHQISLDVWEPPTARISVDC
                     SKGTYIRSLARDIGERVGTGAYMSNLVRVRTGPFSLSDAVTIPELEALITTEQWDTIA
                     FHPDTVLQDWPTVVLDVHAATEWRHGKPVELEGVAGGLRLYDSAGEWIGVGQGNESGS
                     HILPTKVVTDVMEE"
     gene            complement(5661..6671)
                     /locus_tag="VGR29_06985"
     CDS             complement(5661..6671)
                     /locus_tag="VGR29_06985"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV2073371.1"
                     /translation="MTSGWRLDAQAAHDALNQLKAATRILMPTHQNVDADSLSSVLAL
                     TIALRQHGLDVVPMVSDGSLPHALEFLPGADEVLLYTRDEVPEYDMLCLADCSDRTRL
                     GRFYKDDPSRLDGRVPIVNIDHHITNARFGVVNIVEPSAASATEIIADVLAVWGTVLT
                     REIAQCLLAGIYGDTLGLRTESTTSRTMRTAADLVDAGANPVPIVDALFRLKPKSSVC
                     LWRKALEGVQWTGELIWTELTEETFAECGAQPSEAEGMVNFLAGTEGSRAAAILFAND
                     HGWRISMRSLPVDVDVAAITAEFGGGGHPRAAGVQIEGGEAEKQHFLSRVAELIAEPR
                     VE"
     gene            complement(6668..7078)
                     /gene="rbfA"
                     /locus_tag="VGR29_06990"
     CDS             complement(6668..7078)
                     /gene="rbfA"
                     /locus_tag="VGR29_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV2073372.1"
                     /translation="MTRRTRQVGELLKEELDDIIRREVKDPRIGFFSITNVEVPPDLR
                     SAHVYISVLGSDEERDSTLAALRSASKFVRYHLKPRLRMRQIPDLEFRDDRSMEYAQH
                     ISETLRKIRAQDEAFRRTHPDGETKDIPSSEESK"
     gene            complement(7117..9006)
                     /gene="infB"
                     /locus_tag="VGR29_06995"
     CDS             complement(7117..9006)
                     /gene="infB"
                     /locus_tag="VGR29_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV2073373.1"
                     /translation="MTDTIGRGTRRGGGNKRGRRGAGGGARTVAVKRAAPVAREPVSL
                     PPVMSVAELADLLETSGIEVIRELMKVGIMANLNQQVDYETAAKVAEELGWETNEADS
                     AMKSTVADFESRRLENDSDPDSRTRPPVITIMGHVDHGKTRLLDSIRSTNVAEGEAGG
                     ITQHIGAYQIESQGRKLTFLDTPGHEAFTAMRARGAQVTDIAILVVAADDGVMPTTRE
                     AVAHIKSAQVPMVVAVNKVDLPAANPDRIKQQLSEMEVMPEEWGGDIPFVEVSAKEGM
                     GIDDLLEVLLLVADVNELKANPVKAAQGVVIEAKIDGSRGPVATLLVQSGTLNMRDVV
                     VAGTTWGRVKAMFDDRGKRIRRAGPSSPVEVMGLMEVPQAGDTFLTFEDEKAGRQLAE
                     QRSAQKRVQHLNENRPIKLTDLYDQVQEGKTAELRVIIKSDVQGSLGAIQNALLKLNE
                     DMEAGVTLSVQLAATGAVTESDVNLAATTGSIVLGFNVRPDVAAKRAADAAGVDVRFY
                     TIIYELIDEVKKAMTGLLAPVYQDVTDGYAEVRETFKLPNNDVVAGLYVLDGKVPRNS
                     RARVLRSGTVLFEGGIKSLKRFKDDVREVAAGYECGLGLEGFNDLKVGDQIEFLHREE
                     VARPS"
     gene            complement(9389..>10157)
                     /locus_tag="VGR29_07000"
     CDS             complement(9389..>10157)
                     /locus_tag="VGR29_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV2073374.1"
                     /translation="RSDARLIRRLFEQEVPEIQSGAVEIMEIAREPGLRSKVAVAARQ
                     EKVDPVGSCVGVRGVRIQNIVNELYGEKIDVIEWSSEVATFIANALSPAKPTNVSLSE
                     ADDQRVATVIVPSDQMSLAIGKEGQNARLAYKLTGWRIDIKGPESLLQAGDDFFRQAR
                     DSSASMMPDFSWQGRQPRAVQADGMVTINEQSYGPLSDDLIRRQVDVDVVDGVVEIYY
                     ERDLRARFDQVSGQRLPLDDADETDYSALSRAAGTEV"
BASE COUNT         2060 a         3125 c         2814 g         2158 t
ORIGIN      
        1 ccggcaaggg gataagcatg cgtatcgcga tcgacgcgat gggaggcgat cacggaccgt
       61 ccgtggtggt gccgggggta cttgcaggag cccggcgcca cggcatttcc ctgctcctcg
      121 tggggcggaa gcaggagttg gagaacagcc ttgccgggga cgatacgtcg ggaatcgatg
      181 ttgagattgt agacgccacc caaaccatcg acatggacga tcaccctgca caggcggtcc
      241 gccgcaagac ggatagttcg atcaatgttg ccctgcgcct ccttaaggag agccgagcca
      301 atgccatgct ttcagccggt aacagcggcg ccgtcatggc ggcctcgctg atgatccttg
      361 gccggatcaa gggtattgag agacccgcca ttacctcggg catcccgaac gccaaaggca
      421 catattcatt gattgtggat ctcggggcag taacagaccc gaagccgaac aacatggtgc
      481 aattcgccgt gatgggccaa gtgtacgcgc aggcggtgat gggtgtcctg aacccgacca
      541 tcggccttgt gagcaacggt gaggaggcca gcaagggtaa cgcactagtg cagcaggttc
      601 atcccctcct cagggccact gaagggctca attttgttgg taatgtcgag ggcaaagaca
      661 tcgcctccgg cactgtcgat gtctttgtga tggatggttt caccgggaac gtcgtcctga
      721 agacgatcga aggcaccgca tccatgctga tgcacctcgt acgtgaggaa attacctcca
      781 cccttcccag gaaactggca gcccttgtgc tcaaaccggc ctttcgccag gttagtggca
      841 agcttgacta tgcagcgatc ggaggcgctc cgcttctcgg agtagatggc accgtcgtca
      901 tctgtcacgg acggtcaagc gagatggcgt ttgaaaacgc tgtcggagtc tgcaagcggg
      961 ctgtggagca cgacctctcg ggtgaaatct atcgacgagt tgcggcaact cacgagtttg
     1021 tgtctgaggc gcacgcaggc acatagcgtc tctactttag aacgaacgag gcccggatcg
     1081 gtgatcgacc cgagcctcgt agatttggac caactggcgg atctactcga ccgcgcgctt
     1141 aagctgagtt cctggacgaa agaccgggct cttgcgagcc ttgatcgtca tctcctcgcc
     1201 ggtttgcgga ttacgaccct gccgagcagc acgctcgacg accttgaagg agccgaaacc
     1261 gctgatcgtc acctcatcgc cgctggacaa cgcgtcctcg atggccgaga agacagcgtt
     1321 cacagccttt gagcttgaac cttcattctg ctgagtcgct tcggcaacgg cacgaaccag
     1381 atcttgcttg cgcacggtat gtctcctcta cccaagtgtc ctctcaagtg aacctcagga
     1441 tggtgtgatc acgaatctac gttccgaata gctatcgacg ctgcgtcagc atactccaaa
     1501 agcccgcatc catgccaaat ctcggccacg cgagagctat tccaatgcat cattcgcgcg
     1561 acgctgacgt gctgcctcaa atagtagcaa tgatcctgcc acgccggcat taaggctttc
     1621 aacgccccct gccaacggaa tggccacccg cgtcgttgca cagcggcggg cggaggccgt
     1681 cggcccaaac gcctcgctac caacaatgag tgctgcagac ctaagccaag aaagccgatc
     1741 atattccgtc tcgccggaag cgtcggccag ggccaccacg tcgtatcgct taaggctcgt
     1801 tgccaattca tcccagtccc ggtgagacag tgccacccgg acatgcgctc ccatagctgc
     1861 acgcaccgca cgggggtggt aaggatccac cgtttccgga gtaatcacaa cgtggtcagc
     1921 ccctgcaccg gcagctgagc gcaagagggt tccgagattg ccggggtctc gaaccgcatc
     1981 aacaatcaac aggagaggag cgcgaccact ggaagcctcg ggcagattcg attcagccgg
     2041 cattggaaca acagccagga ttccttgtgg gtgcggcaca tctgtcaatg aatcgaacac
     2101 aggcgcgatt acccggcgga gcggaacgtc gtcaaggaga tgcggcacca gatccagatc
     2161 gaagtcatca cgaaggtaca cgactcgggg aatcatttcc gcatggatta cgtcccggac
     2221 cgcacggacg ccttcaacga tgaacgctcg ctcctgcagc cggattttcc ggcgctggag
     2281 cgagcgaatc agcttgatac ctggattgga gttgctggcg atcacatctc cggtggaatg
     2341 aatgcagctc atccggatgc taccgcatca ggcttgggcg gatggcaggg aactctttgc
     2401 aacctgaacg accgcggcaa agccctcctc gtcgcgaacc gcgagatcgg ccaggatctt
     2461 tcggtcgacc tcgacgccgg cgagggtgag accgtggaca agatggctgt agggcatacc
     2521 attcaaacgg ctggccgcat tgatccgctg aatccacaac cggcgcatcg ctcgcttgcg
     2581 gttccgacgg tcacgatagg catactggag agcgtgcata agggattcat gcgcgcgctt
     2641 gtaaaggcgg tttcgcgtgc cgcgatgccc cttcacctgc ttgagaacac tacgatggcg
     2701 gcggtgagcc gcgacgccgc gctttactct tgccatattt caactacctc attcattcat
     2761 tcattacgct atggtctagc taatgccatt cgggatgaga cgcttgacgc gcttcaccga
     2821 ggcgtcgttc attaccagca ttcggtcaaa ttgacgcttg acgcgcgcgg acttcttgcg
     2881 gcggttgtgg ctcttcatgc ccttggcgtg catgatcttg ccagtggccg taaccttgaa
     2941 ccggcgcttg gcgcccttat gcgttttcag cttaccctgc ttgctcacgt tgttcctctc
     3001 tatcggacga ttccgctcgt tggcgggacg gcgccatatg catcgtcatg gttcgtcctt
     3061 ccatgcgcgc ggactgttcg atcaccccgt ggtccttcag ctgttcagcc aggcgctcga
     3121 gaagatcttt gcctaactcg ggatgagtga tcgaacggcc tcgaaataga accgtcaatt
     3181 tcaccttatc gccggcatca agaaacttgg cggcctgccg gcccttggtt tcaaggtcat
     3241 gaccgccaat ttttggctcg atccggacct ctttcacctt gatgacgtgc tgggaacgac
     3301 gggaatcacg ctctttacgg ctctgctcgt acttgtactt cccataatcc atcaggcgac
     3361 aaactggcgg cgatgcgttt ggcgcaacct caacgaggtc tagtcctcgc tcacgagcca
     3421 tatctagggc ttcccgcgtg gggatgatcc cgatctgctg gccctcatcg tcaatgagcc
     3481 gcacctcgcg tatacgaatt cgctcgttga cgcgcgtttg tactggtctg gaaatggcta
     3541 tcctcctgaa cacgtatgac ggtgcgcggg gcatggaaaa tcgtcggcca ttacgcccga
     3601 cgcccagcca ccggcaacac cgctcaagtg tactgtaaat gtccccggta tttcaacggt
     3661 ttaactgtct tcgacctctc ccggccggcg tgatgtgagc acctctctcg cgtgctcttc
     3721 gtcccgtgcc agttgggcct tgagtgcgtc cacactgcga aaattggcgt caggccgcag
     3781 gcggcgaaca aattcggttt cgagcgtccg gccgtagaga tctccatcaa aaccgaaaag
     3841 gtgagtctcg atcattcgat caccggtgtt gacggcaggg cgagtgccga tgtaggtcat
     3901 tgcctcgaag atggcgtcat tatctaggat acgcgcgaac gttgcatata tgccgtcctg
     3961 gagcggcacg agatcctccg gaggtaccac attcgccgtg gggaagccga tagtgcgccc
     4021 aacttgcgcg cctcgaacta cctctccacg gacctggaaa cggtgtccca ggatgaacga
     4081 tgcatcgtct ggccgtcctt gcagaatcag gttgcggatc cgtgtgctgc tcacctcctg
     4141 ccccttgtaa tcgactctgg gaacggcgcg cacggtcgtc ccagacgggg cggcaatttc
     4201 ggcgaggcgc gcgggagtgc cgcttctctt gtgcccgaga gcaaagtcgc ttcccaccca
     4261 tatctggcga acaggaccga ccgcgaacaa ctgcgccaca aactcctcgg cgcacatcct
     4321 ggacatctca agcgtaaaag gcaactcaac aaccagatcc gccccatatc ggaacagcaa
     4381 accccggcgc cgttccttgg tgagcacacg accggcaaag gtttcggggc gaagaacctg
     4441 gatcggcaac ggctcgaagg ttaggacgag cagtcgtgat tcatgctcct ttgctccctc
     4501 acgcgccaga cgcaacagat gctgatggcc gcgatggacg ccgtcaaagg cgcctatggt
     4561 tgctacggtc ggcgacgccg tgacatcact gaagcttgcg aatacccttt cagccacgct
     4621 ctttactctt ccatcacgtc cgtcaccact tttgtcggaa ggatgtggct ccccgattca
     4681 tttccctgcc ccacaccaat ccactctccg gcgctatcgt acaaacgaag gccacccgcc
     4741 acgccttcga gctccacggg ttttccgtgg cgccattcgg tggcggcatg aacatcaagc
     4801 acaacggtcg gccagtcctg aagcaccgta tccgggtgaa acgcgatggt gtcccattgc
     4861 tcggttgtta tcaatgcttc gagctcagga atggtgaccg cgtctgaaag tgagaacgga
     4921 cctgtgcgta cacggacgag gttcgacata taggcaccag tgcccacccg ctctccaatg
     4981 tcgcgagcca gcgaccttat gtatgtcccc ttcgagcaat ccaccgaaat ccgtgcggtc
     5041 ggtggttccc acacgtccag cgagatttgg tggattctga taggacgcgg ctccaactcc
     5101 accgactctc cacggcgcgc gagttcgtat agacgttctc cattcacctt gatcgcggag
     5161 tacgccgggg gcacctggaa ctgatcgcca aagaatgatt caagcacctg cacgatatcg
     5221 aattcttgca gacggcttgt atctccaact gacgtgacga caccgtctac atcagcggaa
     5281 tctgtagtta ccccgaatgt gacatcggct atataggcct tggaggctcc ggacagatac
     5341 tcgactgttc gcgttgcaag cccaaccgcg atgggtaaga cgcccacagc agctggatcg
     5401 agtgttcccg catggcctac ccggcgctca ctcaatatgc gacggacgtt tcccacgact
     5461 tgatggctcg tccaaccggc cggtttgtcg atcacgagat atccgtgata caggccgcgc
     5521 gttttcctct ttaccatcag gggagccgac cgcgataaag ggagccttgc ccccataccg
     5581 catccactcg gtccgtgttc attatttcag tttctcggac gcgaaggaca agcgcagcgt
     5641 ggtggaaccc ttggctcatc ctactctacc cgaggttcag caataagctc ggccacgcgg
     5701 gagaggaagt gctgcttttc agcctctcct ccttcgattt ggacaccggc ggcacgcgga
     5761 tgaccaccgc cgccaaactc cgcagttatc gcagctacat cgacatctac aggaaggctt
     5821 cgcatgctga tccgccatcc atggtcgttc gcaaagagaa ttgcagccgc acgagatccc
     5881 tccgtccctg caaggaagtt caccatacct tcagcttcac tcggctgggc gccacactcc
     5941 gcaaacgtct cctcagtgag ctccgtccaa atcagctcac cggtccactg gacgccctca
     6001 agggcctttc tccatagaca gacggatgac ttcggcttta gccggaagag tgcatcgaca
     6061 atgggaaccg ggttcgctcc cgcatcgaca agatcagcag cagtgcgcat agtcctggag
     6121 gtagttgact cggtacgaag tcccagcgta tcgccgtaga ttcccgccag caagcattgt
     6181 gcaatctcgc gcgtgagtac cgtaccccat acagccagta cgtcggcgat gatttcagtg
     6241 gcagacgcag cacttggctc gactatattg acgactccaa agcgcgcatt ggtaatgtgg
     6301 tggtcgatgt taacgatagg cacacgtccg tcgagacgac ttggatcatc cttgtagaac
     6361 ctgccgagcc gcgtccggtc cgagcaatcc gccaggcaga gcatgtcgta ttcaggcact
     6421 tcgtcgcggg tgtacagcag cacctcgtca gcccctggca ggaattcaag cgcatgcggt
     6481 agcgatccgt cactgaccat cggcacgacg tcgagaccat gctgacgtaa agctatcgtc
     6541 agtgcaagca ctgacgatag tgagtcggcg tcgacattct gatgcgttgg catcaaaatc
     6601 cgcgttgccg ccttaagctg gttcagtgca tcgtgtgcag cctgggcgtc caacctccac
     6661 ccggatgtca ttttgattcc tcggagctgg ggatatcttt ggtttccccg tccgggtgag
     6721 tgcggcggaa agcttcatcc tgcgcccgga tcttgcgaag ggtttcggag atatgctgcg
     6781 catactccat cgatcggtcg tcacggaact ccagatcggg gatttgccgc attcggaggc
     6841 ggggcttgag gtgatagcga acgaatttcg acgccgagcg taacgccgcg agagtgctgt
     6901 cacgctcctc atccgagccc agcacgctga tgtaaacgtg ggcggaccgg agatccggcg
     6961 gcacctcgac atttgtgatg ctgaagaacc ctatccgcgg gtccttgacc tcgcgccgga
     7021 tgatgtcgtc gagctcctct ttcaggagct cgcctacttg tcgtgtccgt cgtgtcacag
     7081 tcgttctcgt gtctcttgcg agcagcaggg aaccggctac gagggccggg ccacttcctc
     7141 gcgatgcagg aactcgatct ggtcgccgac cttcaggtcg ttgaatcctt cgagccccag
     7201 tccacactca taccccgccg caacttcgcg tacatcgtcc ttgaagcgct tcaatgattt
     7261 tatgccacct tcgaagagaa cggtgccgct gcgcaacaca cgagcgcgcg agtttcgagg
     7321 aaccttgccg tccagtacat aaagacccgc cacgacatcg ttgttgggta gcttgaacgt
     7381 ttcccgcacc tcggcataac cgtcggtcac atcctggtac acgggagcga gaagaccagt
     7441 catggccttc ttcacttcat caatcagctc gtagatgatc gtgtagaagc ggacatccac
     7501 accggcggcg tcagcggcgc gttttgcggc cacatccggg cggacgttga agcccagcac
     7561 aatacttccc gtcgtcgcag ccaggttgac gtctgactca gtcactgctc ctgtcgccgc
     7621 aagttggacg gaaagcgtaa cgcctgcctc catgtcctca ttcagcttca gcagcgcatt
     7681 ctgaattgcg ccgagcgaac cctggacatc tgacttgatg atgacgcgca gctctgccgt
     7741 cttgccttcc tgcacctgat cgtagagatc ggtcagcttg atcggacgat tttcgttgag
     7801 gtgctgaaca cgcttctggg cagatcgctg ctcggcaagt tggcgtccgg ccttctcatc
     7861 ctcgaaagtg aggaacgtat cgccggcctg cggaacttcc atcaggccca tgacttcgac
     7921 cggtgagctc ggaccggcgc gacgaatgcg cttgccgcga tcatcgaaca tcgccttcac
     7981 acggccccag gtggtgcctg caacgacgac gtcgcgcata ttgagcgttc cactttgtac
     8041 gagcagcgtc gccaccggac cacggcttcc gtcgatcttc gcctcgatga cgacgccctg
     8101 cgccgctttg accggattcg cctttaactc gttgacgtcg gcaacgagca agagcacttc
     8161 aagcaaatca tcgatgccca tgccctcctt cgcggagacc tcgacgaacg ggatgtcacc
     8221 tccccattct tccggcatga cctccatctc ggaaagctgc tgcttgatcc gatcaggatt
     8281 ggccgctggc aggtcgacct tattgacggc cacgaccatt gggacctgcg cggatttgat
     8341 atgggcgacg gcctctcgcg tggttggcat gacgccatcg tcagcggcaa ccaccagaat
     8401 agcaatgtca gttacctgtg caccccgggc gcgcatggcg gtgaatgctt cgtgaccggg
     8461 ggtatcgaga aaggtcagct tccggccctg gctttcaatc tggtacgcgc caatatgctg
     8521 ggtgatccca cccgcctccc cttccgcaac attggtcgag cgaatggagt caagaaggcg
     8581 cgtcttgccg tgatcgacgt ggcccatgat cgtgatcacc ggcggccggg ttctggaatc
     8641 agggtcgcta tcgttttcca ggcgccggct ctcgaagtca gcaaccgttg acttcatcgc
     8701 cgagtcggcc tcgttggttt cccatccgag ttcctcggct accttcgcag ccgtctcata
     8761 atcgacctgc tgattcaggt tggccatgat gccaaccttc atcaattccc gaatgacttc
     8821 gatacccgag gtctcgagga gatcggcaag ttcagccacg gacatgacag gaggcaagga
     8881 taccggctcg cgggcgacgg gtgcagcgcg cttgacggcc actgtacgag caccgccccc
     8941 ggcacctctg cgtccacgct tattgccacc accccggcgt gttccgcgtc caattgtgtc
     9001 ggtcatagtg cttgctcctt cggtcccacc gtggtcgcga catcatccgt ccggagaggc
     9061 gcaccagcgg caaattccct tatcttgatt agcgattccg gtgtcggagt cgttttcagt
     9121 gccttcgcca ggattggcgt cgtcacagcc cgctcccaac acgtttgctt gtcacacaaa
     9181 tacgcgcctc ggccattcat gcgaccgggc ggatccacct caatctgccc ctctttggtg
     9241 cgtacaatcc gcgtcagcgc ccgcttcgcg cttgtgtcgc ggcagacgat gcaggttcgc
     9301 tgaggaacat gcttgaggcg gggacctgac ttcttcgacg atttcggctg cttaagtccg
     9361 gtttgaggca tacgttgttt cgccttcttt agacctcagt gccagccgct ctcgacaggg
     9421 cgctgtagtc cgtttcatca gcatcgtcaa gcgggagacg ctggccggag acctgatcga
     9481 accgggctcg taggtcccgc tcgtagtaaa tctccacgac gccgtcaacc acgtcgacat
     9541 caacttgacg tcgaatcaaa tcatcggaca gcggtccata ggactgttca ttgatggtca
     9601 ccataccgtc ggcctgcact gctcgtggct gacgcccctg ccagctgaag tcaggcatca
     9661 tgcttgcaga cgaatcccgc gcctgccgga agaagtcatc accagcctgc agcagggatt
     9721 caggtccctt gatatcaatg cgccagcccg tcagcttgta agccaatcgg gcattctggc
     9781 cctccttgcc gatcgcaagg gacatctgat cgctcggcac tattactgtc gccacccgtt
     9841 gatcgtcagc ctcgctgaga gacacgttgg ttggtttggc ggggcttagg gcattggcaa
     9901 tgaacgttgc aacctctgaa gaccattcaa taacgtcgat cttctcgccg tacagctcgt
     9961 taacaatatt ctggatacgg acaccacgga cgcctacgca agaaccgacc ggatcgacct
    10021 tctcctgtcg ggcagcaact gctaccttcg agcggagacc cggctctcgt gcgatctcca
    10081 taatctcaac agcgccactc tgaatctcgg gcacttcctg ctcgaacagc cggcgaatga
    10141 gccgggcatc tgaacgg
//
LOCUS       DASYWM010000169         6441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_156880, whole genome shotgun sequence.
ACCESSION   DASYWM010000169 DASYWM010000000
VERSION     DASYWM010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156880"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VGR29_07005"
     CDS             complement(<1..707)
                     /locus_tag="VGR29_07005"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEV2073375.1"
                     /translation="MTNPQDQLLSSTHRLMPSPSRAFSRRVLVCGVAAAAMTPSLRAG
                     AAHDGHGTPAASATPATAVEGEADAVALLEAAAGALAALETFGFELATTRGSSTILEG
                     FELKEVAGVVRRPLDVEAEVTVGLPIGEITITAVGLDGQFWVQDPLSDGEWMSFNSDP
                     QIQSLINPTVLVTNAVRLVQDAQITGNEEVDGVETTVVEGTVDFSSSLGSIASAEGES
                     EALQQFLAEGARDVTFW"
     gene            955..1731
                     /locus_tag="VGR29_07010"
     CDS             955..1731
                     /locus_tag="VGR29_07010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2073376.1"
                     /translation="MISSDQSRPTVHTLVADEMPRLAAALGDSPETVIAHYLLVTGAC
                     NAWCVDGIDGLTAAVVQAELMPAEPTAFGTSAEDIVRIISHVAGWTSILVPAALARSL
                     ERPIAVAAGTLAINTLEDVYHVLDGPVATFDRHPDVRLLTPDDAELLGDLGALSSTDT
                     GEMIVAAAIVEDQIVSLAHTFAWSPAHVDIGVTTHEEWRGHGYATSAAAIVSEEILKQ
                     GRTPVWSCGAHNEPSMRIAARLGFRETSRKVYIIPLKKDS"
     gene            1741..3210
                     /locus_tag="VGR29_07015"
     CDS             1741..3210
                     /locus_tag="VGR29_07015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073377.1"
                     /translation="MAFITGSGVGSIRRPRPDVRAMEEHNGMSPDQQPEPSIWDASNL
                     ALTLGSILAVTLGAFQGLAVATIAPVLADDLNGRGLYGWIFTSFLLPQIIGTVLAGRE
                     VDRRSPAVVFYTAMALFALGCFLAGAAPNIWVLFAGRALQGLGAGGTFATVYAIISGA
                     YEDRLRPSMLAAISSAWVIPSLIGPVIAGFIADHYSWRYVFWGLLPILAIIAPLTLPS
                     YRKISLERDPDAVVNPRRVPYALVLAVGTGLFLAGPDLRPLPLGAMVTLAGLVLLIPV
                     LQRLLPAGTFSARPMIGSALATRSLCFGAFAVTETYMVFSLTEFGGVSTATAGIVLTF
                     GSLTWTSGSILQARLDRQHGPGSRTARARAGVTIMLAGVGIIFGTIVIFDDIWVIIAA
                     LGWMMTGLGIGLAYSTSTTVAFANTPRGQDGMVSSSTLLCDLFTSSVGVGLGGVLLAF
                     TLGLDWSAPPSAALSMSLGVVMLAMAWLAAWRMSTSRTA"
     gene            complement(3434..5254)
                     /locus_tag="VGR29_07020"
     CDS             complement(3434..5254)
                     /locus_tag="VGR29_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2073378.1"
                     /translation="MGFIMHGLDAEDYDRKYDDRVLVSRIGTYFRPALVAMAVVALLI
                     VLNSVASMLNPLLASWGIDRIADGDAGEVIWILFFGCLLAGVLAWGFNFVRQWMTAKV
                     VGDVVLKLRQDAFDAVMARDLSFYDTNPSGKIVSRVTSDTDEFANVVTLTMNLLSQIL
                     LVIIITAVLFTRNVTLAFVAIAITPIFVITALAFRKIARESTQRAQRSTAQVNSSIQE
                     AMNGIAIAKNFRQERTLFEEFVPVNEQSYKVTLKQGFVFQAIFPMLFFMAGLGTVLLV
                     YMGGNQAISGEISPGDWFLFFQSVMLFWMPLTSIASFWSQFQQGLGASERIFALIDAE
                     PEVVQVDDQDPGRIEGSIEFRNLTFAYDDGTNVLASFDLTIPAGQTLAIVGHTGGGKS
                     TLGKLITRFYEFQGGQLLIDGRDVRTFDLPRYRKQLGIVPQSPFLFSGTVADNIRYPK
                     PEASDEEVVRAATLVAGGDWIDALQDGLQTDVGEYGRSLSMGQRQLVALARILIQGPA
                     IVILDEATASVDPLTEAQIQEGLDIVLADRTSIVIAHRLSTIEHADRIIVLDHGEIVE
                     EGTHSSLLARGGRYCEVYNTYFRHQSPNYVPGEGFVPTLR"
     gene            complement(5257..>6441)
                     /locus_tag="VGR29_07025"
     CDS             complement(5257..>6441)
                     /locus_tag="VGR29_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2073379.1"
                     /translation="QFGVVNADLNEAVRGIEVIKATGQEAQEKRKFYRHARLYRDYSV
                     QQGLVQARYLPTLLLAVTVASALLHGVWLVQNEQISVGDLVAFMGLIQQLSFPTFIST
                     FSFSMVQLGLASARRILSLMDEKTELDQNAGGYSGTITGDVIFDDVTFSYEGEPVLRN
                     VSFHAQPGETVAIVGETGSGKSTLTKLVNRIHDVDSGRILIDGIDVRDWNLDSLRSQI
                     STIEQDVTLFSRSVAENIGFSLGQQADREQVVQAARDAQAHEFIVALEKGYDTVIGER
                     GVTLSGGQRQRLAIARALLTSPQMLILDDSTSAIDSATEDEIQKAIRRLLEGRTTLLI
                     THRLSQIRWADQVLLLRKGELVAHGSHEDLLEKSRLYRRIFAHYDEIEDTAAPAVAGG
                     EA"
BASE COUNT         1189 a         2098 c         1883 g         1271 t
ORIGIN      
        1 atccagaagg tcacgtcccg cgccccttcc gccagaaact gctggagcgc ctctgactca
       61 ccttcggcac tggcgattga tccaagcgag gaggagaagt cgactgtgcc ttccaccacg
      121 gtcgtctcga cgccgtccac ctcctcgttg cctgtgatct gggcatcctg aacgagacgg
      181 accgcattcg tgacaagtac cgtaggattg atcagcgact ggatctgcgg atcactgttg
      241 aaagacatcc attccccgtc gctcaacgga tcctggaccc agaactggcc gtccaggccg
      301 accgccgtga tggtgatctc gccgatcggc agcccaaccg tcacctcggc ctcaacgtcc
      361 agagggcggc gcaccacccc tgcgacctcc ttcagctcaa acccctcgag aatggtgctt
      421 gagccccggg tggtggccag ctcaaagccg aaggtctcga gcgccgcgag tgcgccggca
      481 gctgcttcaa gcagggccac cgcatcggct tcgccctcaa ccgcggttgc cggtgttgcg
      541 gatgcggcag gagtcccgtg gccgtcatgg gcggcgcctg cgcggagcga tggagtcatc
      601 gccgcggcgg cgacaccaca gacaagcacc cgacggctga atgcccggct aggtgaaggc
      661 atcaggcgat gcgtcgatga gagcagttga tcctgagggt tcgtcatgtg tacgctcgct
      721 ttcatgaggg aatgttcgac cacttcgtat cgggatggac aagaagcctt tgcttgctgc
      781 tactgccatc ctgctttgcc ccggaacaca ggtttcggcg cgaagacggt gttctcataa
      841 aaaacctttc atgtgcgatc acgaaatgtt gaagtccgtg tcaatctatc ccatgcttgc
      901 gcatgatccc gcggccaccc cgcgaatatc ctcatccatc tcaaaggacc acaggtgatc
      961 tccagcgacc aatcccgtcc cactgtccac acccttgtcg cggatgagat gccgagactt
     1021 gccgctgctc ttggggattc gccggaaacc gtcatcgccc attatctcct ggtgacgggg
     1081 gcgtgcaatg cctggtgcgt cgacggcatc gacggcctga ccgctgccgt ggtacaggcg
     1141 gagctcatgc ctgccgaacc cactgccttc ggcaccagtg ccgaggacat cgtccggatc
     1201 atctcgcacg ttgcgggctg gacctcgatc ctggttccgg cggcgctggc caggtccctg
     1261 gagcggccga tcgccgtcgc cgccggaacc ttggcaatca ataccctgga agatgtctat
     1321 cacgtcctcg acggaccagt cgccacgttc gaccgccatc cggacgtgcg cctcctgacc
     1381 ccggatgatg cggagctcct tggcgacctc ggagccctct cttcgacgga cactggagag
     1441 atgatcgtcg ctgcggcaat cgtggaggat cagatcgtct cccttgccca tacctttgcc
     1501 tggagccccg cccacgtcga cattggcgtc acaacccacg aggaatggcg cggacatgga
     1561 tatgctacca gtgcggctgc cattgtctcc gaggaaatcc tgaagcaggg ccgcacgccg
     1621 gtatggagtt gcggtgccca caacgagcca tcgatgcgca tcgcggcccg gctcggcttc
     1681 agggaaacga gccgcaaggt ctacatcatc ccgctcaaga aagactcgtg agccaatcgt
     1741 gtggcattca tcaccggctc cggggtaggc tccattcggc gcccgcgtcc tgacgtgcgg
     1801 gcaatggagg agcacaacgg catgtcgccg gatcagcaac ctgaaccgtc catctgggac
     1861 gcatccaacc tggctcttac gcttggctcg attcttgctg tcacgcttgg tgcctttcag
     1921 gggcttgcgg ttgcgacgat tgccccggtt ctcgccgatg atcttaacgg ccgcggtctc
     1981 tacggctgga tcttcacctc cttcctcctg ccgcagatca taggaaccgt ccttgccgga
     2041 cgggaggttg accgccggtc accagcggtc gtcttctaca ccgccatggc tctcttcgct
     2101 ctcggctgtt tccttgccgg agccgcgccg aatatctggg tgctgtttgc cggccgcgcg
     2161 ctgcaaggcc tcggtgcagg gggaacattc gccactgtct atgcgatcat cagcggcgcc
     2221 tacgaggacc gcctgcggcc atcgatgctc gcggcgatct ccagcgcctg ggttatcccg
     2281 tcgcttatcg gtcccgtcat cgccggcttc attgccgatc actacagctg gcgatacgtc
     2341 ttctggggat tgctcccgat cctcgccatc atcgcaccgc ttaccctgcc ctcctacagg
     2401 aagatcagtc tggagcggga tcccgacgcg gtggtgaacc cccggcgagt cccctatgcc
     2461 cttgtgctgg ccgttgggac tgggctcttc ctggctggcc ctgatctccg gccgcttcct
     2521 cttggcgcaa tggtcacact tgccggcctg gtgctcctga tcccggtgct tcagcgcctg
     2581 ctacccgcag gcacgtttag cgcccggccg atgatcggat ctgctcttgc cactcgctcg
     2641 ctgtgtttcg gggcgttcgc cgtcaccgag acctacatgg ttttctccct cactgagttc
     2701 ggcggcgtta gcactgcaac cgcgggcatc gtgctcactt tcgggtccct gacgtggaca
     2761 tcgggatcga tcctgcaggc acggctggac cgccagcacg gaccgggcag taggacagcc
     2821 cgcgcccgcg ccggcgtgac catcatgctc gctggcgtag gcatcatctt cgggacgatc
     2881 gtgattttcg atgatatctg ggtcatcatc gccgcgctgg gatggatgat gaccggcctc
     2941 ggtattggcc ttgcctacag cacgtcgacg acggttgcct ttgccaacac cccgcgtggg
     3001 caggacggca tggtgtcgtc ttctaccctc ctgtgcgatc tctttacgtc gtcggtagga
     3061 gtgggactgg gcggcgtact gctcgccttc accctcggcc ttgattggag cgcgccaccg
     3121 agcgcggccc tgtcgatgtc gctgggagtg gtgatgcttg ctatggcgtg gctggcggcc
     3181 tggcggatga gcacctcccg cacagcttag cgctattctc aaagaggctg catccctgga
     3241 gtgtctgtag cgaaggggct cgctgccgca cggcaaaaag cggcagtgtg ttcttcgcta
     3301 caccgccggc cggcggcttg gcggatgagc acaaccgcac ggcggagaga attaccgtcc
     3361 accgctacgc caagctatat ttccatcgtg ccaacaacgg tggtccttcc tgctgcactg
     3421 ggaaggaccc atctcacctc agcgttggga cgaacccctc gccaggtacg taattcggtg
     3481 actgatgccg gaagtacgtg ttgtagacct cgcagtaccg gccacccctg gcaaggagcg
     3541 aggaatgcgt gccctcctcc acgatctcgc cgtgatccag cacgatgatc cggtcagcat
     3601 gctcgatcgt cgacagccgg tgggcaatga cgattgaggt ccggtccgca agcacgatgt
     3661 ccaggccttc ctggatctgc gcctcagtga ggggatcgac actcgccgtc gcctcatcga
     3721 ggatcacgat cgccgggccc tggatcagga tccgcgccag tgccacaagc tgcctttggc
     3781 ccatcgagag cgaccgcccg tactcaccaa cgtccgtctg cagcccatcc tgcaatgcgt
     3841 cgatccagtc gcctcccgca accagcgtcg cggccctcac cacctcttcg tcgctggcct
     3901 ccggctttgg atagcggatg ttgtccgcaa cggtcccgga gaagaggaac ggcgactgcg
     3961 gcacgatgcc cagctgcttt cggtaccggg gcaagtcgaa cgtgcgaacg tccctgccgt
     4021 cgatcagcag ttgcccgccc tggaactcat agaatcgggt gatcaacttg ccgagcgtcg
     4081 atttcccgcc acccgtgtgg ccaacgatcg ccagcgtctg ccccgccggg atcgtcaggt
     4141 cgaagctggc tagcacgttt gtgccgtcat cgtaggcaaa ggtcaggttc cgaaactcga
     4201 tgctaccctc gatccgtccc ggatcctgat cgtcaacctg gacaacctcg ggctcggcat
     4261 cgatcaacgc gaaaatgcgc tccgacgcgc caagcccctg ctggaactgc gaccagaacg
     4321 acgcgatgct ggtgagcggc atccagaaga gcatcacgct ctggaagaag agaaaccagt
     4381 caccaggcga tatctcgcca gagatggcct gatttcctcc catgtacacg agcagcacgg
     4441 ttccaagacc tgccatgaag aagagcatcg ggaagatcgc ctggaagacg aacccctgct
     4501 tcaatgtcac cttgtaggac tgctcgttga ccggtacgaa ctcttcgaag agtgtgcgct
     4561 cctgccggaa attcttcgcg atcgcgatac cgttcatcgc ctcctgaatg ctggagttca
     4621 cctgcgcggt cgaacgctgg gcacgctgcg tcgattcccg cgcaatcttc cggaacgcca
     4681 acgccgtgat gacgaagatg ggagtgattg caatcgccac gaaggcgagc gtcacgttcc
     4741 gggtgaagag caccgcggtg atgatgatca ccagcaggat ctggctgaga agattcatcg
     4801 tcagggtgac gacgttcgca aactcatccg tgtcggaggt cacgcggctg acgatctttc
     4861 cagagggatt ggtatcgtag aaggagagat cgcgggccat caccgcatcg aaggcatcct
     4921 gtcgcagttt cagcacaaca tcgcccacga cctttgcggt catccactga cgaacgaagt
     4981 tgaaacccca cgccagcaca cccgcaagga gacaaccgaa gaagagaatc cagatcacct
     5041 cacctgcgtc gccatcggcg atccggtcta tgccccagct cgccaggagg ggattcagca
     5101 tactcgcgac cgagttgagg acgatcagaa gggcgaccac cgccattgcc acgagagctg
     5161 gccggaagta ggtgccgatc ctgctgacaa gtaccctgtc gtcgtatttg cgatcgtagt
     5221 cctcggcatc gaggccgtgc atgatgaatc ccacggctat gcctccccac ccgcaactgc
     5281 aggcgctgct gtgtcttcga tttcgtcgta atgggcgaag atgcgacggt agagccggga
     5341 cttctccagc aggtcctcat gcgagccgtg ggcgactagc tcacccttcc ggagcagcag
     5401 cacctgatcc gcccagcgaa tctgggatag ccggtgcgtg atcaacaagg tcgtccggcc
     5461 ctcgagcagg cgccggatcg ccttctggat ctcgtcctct gtggcgctgt cgatcgcact
     5521 tgtggaatcg tcgaggatca gcatctgcgg cgaggtcagc agtgcgcggg caatcgccag
     5581 gcgctggcgc tggccacccg aaagcgtcac cccacgctcg ccgatgacgg tgtcataccc
     5641 cttctcgagc gcgacgatga actcatgcgc ctgggcgtcc cgggccgcct ggaccacctg
     5701 ctccctgtcg gcctgctgac cgaggctgaa gccaatgttc tccgcgacac tgcgcgagaa
     5761 aagtgtgaca tcctgctcga ttgtggagat ttgggagcgc agcgagtcaa ggttccagtc
     5821 ccggacatct atgccgtcga tcaggatgcg tccgctgtcc acatcgtgaa tccgattgac
     5881 gagcttcgtc aaggtgctct tgccggagcc agtctccccc acgatcgcga ccgtctcgcc
     5941 cggctgcgca tgaaaactga cattccgtaa tacaggctca ccctcgtagc tgaaggtcac
     6001 gtcatcgaag atcacatcgc ccgtgatcgt gcccgagtag ccgccagcgt tctggtcgag
     6061 ctccgtcttt tcatccatca gcgacaggat gcgccgggca ctcgccagac caagctgcac
     6121 cattgagaat gagaatgtcg agatgaaggt cgggaaactg agctgctgga tcaaccccat
     6181 aaaggcgacc agatccccga cgctgatctg ctcattctgc acaagccaga caccgtgcag
     6241 gagtgctgaa gcgacggtta ccgctagcag cagcgtcggc aggtaccgtg cctggacaag
     6301 cccctgctgc accgagtagt cccggtagag tcgcgcgtgc cggtagaact tccgcttctc
     6361 ctgggcttcc tgccccgtcg ccttgatcac ttcgataccg cgaacagcct cgttcagatc
     6421 ggcattgacg acgccgaatt g
//
LOCUS       DASYWM010000170         4303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_663351, whole genome shotgun sequence.
ACCESSION   DASYWM010000170 DASYWM010000000
VERSION     DASYWM010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663351"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VGR29_07030"
     CDS             complement(<1..1202)
                     /locus_tag="VGR29_07030"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEV2073380.1"
                     /translation="MAIIADDVTGAADTGAAFAQMGLATTLAFADEPLSAVDILVRST
                     ESRGLDVSAAALANERAAMALTAHSGGRQIRWVYKKIDSALRGYPAEELLAVMAGLGE
                     TRAVVACALPSEERTTVGGRQYVRGFPLEAGPLGQSNVPCDLVALFGQSHDIVVRSLG
                     LAVIRQGVSAIQRFLRNAGSGVVVADAETDLDLAVLASAIAGSDVRVLCGSAGFARQL
                     AGALPLMPLVQPSIRPPRGTGPVLVVAGSQHPATTAQVDVLGRAGHPAIHLDQDLIDD
                     PSTSIDGTVAKVAMQLSAGRSPVLTTVGLALSPLGESFVVERLSEIVAAPPVRRHLGG
                     LVLTGGHVAAGIFVRLRASALRLGGEIRPAMPWGVLDAELVRSVPVATKAGSFGASDA
                     LLACVSHLG"
     gene            complement(1234..2259)
                     /gene="pdxA"
                     /locus_tag="VGR29_07035"
     CDS             complement(1234..2259)
                     /gene="pdxA"
                     /locus_tag="VGR29_07035"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEV2073381.1"
                     /translation="MTPTDRPLLAITMGDPAGIGPEVVLRSLLDPDVYQRARPLVIGD
                     RQVLERAAPWVGAAHLEYDVISHPADGRYEPGMVTMLDLGLSPLDRYPVGRLTAEAGR
                     AAVEYVFHACDLVMAGNADAVVTAPLNKAAMHMAGFRYPGHTELLAERTGAERVSMLL
                     VGPTLRVIHVSTHVALTEAIRRVTRQRVEEVLETANTACRTLGITHPRIAVAGLNPHA
                     SEDGLFGEEEAQEIIPAIEAARTRGLDVSDPQPPDTVFLRASKGEWDIVVAMYHDQGH
                     IPMKLLAFDSGVNVSIGLPIIRTSVDHGTAFDIAGTGRASEESMLAAITVAIQMAEAR
                     PPAHRGG"
     gene            2615..2842
                     /locus_tag="VGR29_07040"
     CDS             2615..2842
                     /locus_tag="VGR29_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073382.1"
                     /translation="MGQTVCRMGFAEFYVVREEWVRYELGEIAHIDSELADEMKVRTQ
                     VRTTGEQEEWTTDRLFSVSAAATSRRSHCLV"
     gene            3324..4244
                     /locus_tag="VGR29_07045"
     CDS             3324..4244
                     /locus_tag="VGR29_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEV2073383.1"
                     /translation="MRFPAQKRARIIVNTDAKNEADDQFAILHALLTPSFDLHGIIPA
                     HFGDRRSNDSLRESHEEVMHLLRLMGWTNRVQVEDGAARAMPDDSTSVPSAGATRIVQ
                     EALRDDPRPLHVAFYGPLTDMASALILEPRIQDRNVRVVWIGGGPWPNGGPEFNLSND
                     IHAANVVMRSRVEVWQIPSSVYRLMAVSYAELLERVYPMGELGRYLVEQVVDFNARHV
                     SGPIEHRSLGDSPAVGVVMYPDCGRSEWRPAPEFDESMHYVHTGQHRPIRVYETVDAR
                     FVLEDFYAKLKRFAKGETDVPEHIASLASQ"
BASE COUNT          808 a         1353 c         1331 g          811 t
ORIGIN      
        1 ccgaggtggc tcacacaggc cagcaacgcg tctgaggcgc caaagctgcc ggcctttgtc
       61 gccaccggca cggaccggac gagctcagcg tccagcactc cccacggcat ggcgggacga
      121 atctctcctc ccagtcgcaa cgccgacgct cggagacgaa cgaatatccc cgctgccacg
      181 tgaccaccag tgagcaccaa ccctccaagg tgccgccgca ccggtggagc cgccacgatc
      241 tccgaaagtc gctcgacgac gaacgactcg cctagcggtg acagtgcaag accgaccgtc
      301 gtcaacaccg gcgagcgtcc ggcactgagt tgcattgcca ccttggccac ggttccatcg
      361 atggacgtcg agggatcgtc gatcaggtcc tgatcgagat ggatggccgg atgtccggca
      421 cgaccgagca cgtcaacctg ggcagtcgtt gccggatgct gacttccggc cacaacgagg
      481 accggtccag taccgcgcgg gggacggatg gagggctgta ccaggggcat caagggaagt
      541 gcgccggcta actggcgagc gaatccggcg gaaccgcaga gcacccgtac gtcgcttcct
      601 gctattgcac tggcgagcac cgcgaggtcg aggtcagtct cggcatccgc aaccaccacc
      661 ccagaacccg catttcgcag gaagcgctgg atcgcgctga caccctgccg aatgacagca
      721 aggccaagtg atctcaccac aatgtcgtga ctctgcccaa agagagctac cagatcgcag
      781 gggacgttgg actggccaag aggaccggcc tccagcggaa agccacgcac gtactgccga
      841 ccgcccaccg tcgtcctctc ctcggatggc aacgcgcagg ccacgaccgc ccgggtttca
      901 ccaagcccgg ccatgacagc gagcagttcc tcggcggggt agcccctgag ggcactgtca
      961 atcttcttgt aaacccagcg gatttgccga ccaccggaat gggccgtcag cgccatggct
     1021 gcgcgctcat ttgccagcgc agcggcactg acgtcgagac cgcggctttc agtggagcgg
     1081 acaaggatat cgactgctga cagaggctca tccgcgaacg cgagcgtggt cgccagaccc
     1141 atctgggcga aggcggcacc ggtgtcggcg gcgccagtga cgtcatcggc gatgatggcc
     1201 acctggatgg tgctcggccc ggatgccggt tggctaccct ccacggtggg ccggtgggcg
     1261 ggcctcggcc atctggatgg cgacggtgat cgcggccagc atactctcct cgcttgcccg
     1321 tccggtgccg gcgatgtcga atgccgtgcc atggtcaacc gaggtacgga tgatcggtag
     1381 gccgatgctg acgttcactc ccgagtcaaa tgccaggagt ttcatgggga tgtgcccttg
     1441 gtcgtggtac atcgcgacga cgatatccca ctcccccttg cttgccctga ggaagaccgt
     1501 atcgggcggc tggggatcgc tgacgtcaag accacgggta cgagctgcct ctatcgcggg
     1561 aatgatttcc tgagcttctt cctccccaaa gagaccgtcc tcgctggcat gcggattgag
     1621 gccggcgacg gcgatccgag ggtgagtgat gccgagcgtt ctgcaggcgg tattggcggt
     1681 ctccagcacc tcttcgacgc gctgccgggt gacccggcga atcgcttccg tcagcgcaac
     1741 gtgggtgctg acatggatca cgcgtaacgt ggggccgaca aggagcatgc tgacccgctc
     1801 ggcgccggtc cgctccgcca ggagctccgt atgacccgga tagcgaaagc cggccatgtg
     1861 catcgccgcc ttgttgagtg gtgcggtgac aaccgcatca gcgttccccg ccatgacgag
     1921 gtcgcaggcg tgaaagacgt attcgaccgc ggctcgccct gcctccgccg tcagccgtcc
     1981 taccgggtat ctgtccagtg gtgacaaccc cagatcgagc atggtcacca tgcctggttc
     2041 ataacgacca tcggcgggat gcgaaatgac gtcatattcc aggtgggcgg ctcctaccca
     2101 cggagccgct cgttccagta cctgccgatc accgatgacc agcggccgtg cgcgttggta
     2161 cacatcagga tcgaggaggg acctgaggac cacttccggg ccgatcccgg ccggatcgcc
     2221 catggtgatc gcaagcaacg gccgatctgt cggtgtcaac ctgtcctcca tccggtgctt
     2281 gaacggcccg tgatccccat tcggcgtcga tcagcagcgt tgatgccact atggtcggga
     2341 gaacggcagg ccactacctc gcgtacgtcg tgaccatgcg tgtgaccgag agtccccgaa
     2401 cgtcagtcta tagatcgcat gacctcggga caagccaccg atggagggag cacatgcccg
     2461 ggccgagggt gcgacgctca tccccgcccg tgcaaccatg ggtgccgatt ctggtcacgg
     2521 ttctcgatcg ccaccttgcc gatgccagcc gtccgccgca agccgtactt cgcactggct
     2581 tgctggccgc cttcctcgcc ggggaccgag cggcgtgggg cagacggtct gccgcatggg
     2641 cttcgccgag ttctacgtcg tccgggagga gtgggtgcgc tatgaacttg gtgaaatcgc
     2701 gcacatcgac tcggagctgg ccgacgagat gaaggttcgg acccaagtgc gcaccaccgg
     2761 cgagcaggag gagtggacca ccgaccgcct attctccgtc agtgctgctg ctacttcgcg
     2821 tcgctctcat tgtctcgtgt gatacaccat acgcttcggc aagttctcta aggttgcgat
     2881 gaggttgctg ctgaatgtcc tttagttggt ctggagtgag cttccggggt cggcaacgtc
     2941 cacggggtgt ttcctcgcgt ggcaccaggt cagaaacgag cacgcgacgg ggacggaact
     3001 gttctaacat ggcggacgga cacccgttgc ttccgatttt acataaagta tcttccggat
     3061 tgacagcaat cgactcgaag aaggatccca ttctgggccg cggctcgcaa gccacggcct
     3121 gtttgcttgt aattaaggtt ctcgcgaata cctctcgcgc aagaagataa ggctcctcag
     3181 ctgtgacccc tcctgaagcg tgggccatgt gacatgcttt cgccgtctgc ttcattcatg
     3241 aggtaacggc aatcatcgcc ggatttgagt ctggtatcgt gcattgtacc gacccggtag
     3301 caagcgagga gttgatcgtg gacatgcggt ttcccgccca gaagcgggca cggattatcg
     3361 tcaacacgga tgccaagaac gaggcggacg atcagttcgc catccttcac gccctcctca
     3421 ccccgtcgtt tgatcttcac gggatcattc cggcgcactt cggcgacaga cgatccaacg
     3481 actcgttgcg ggaaagtcac gaggaggtaa tgcacctgtt gcggttgatg gggtggacga
     3541 accgagtgca ggtcgaggac ggtgcggctc gggccatgcc cgacgactct acttctgtcc
     3601 catcagccgg tgccacgcgc atcgttcagg aagccttgcg tgatgatccc cggccgttgc
     3661 acgtggcgtt ctatggccca ctgacggaca tggccagtgc gctgatcttg gaaccgcgca
     3721 ttcaggatcg caacgtaagg gtcgtctgga tcggtggtgg accctggcca aatggcgggc
     3781 ccgaattcaa tctctccaac gacatccatg ctgcgaatgt cgtgatgcga tcccgtgtcg
     3841 aggtgtggca gattcccagt tcggtctatc gtttgatggc cgtcagctac gccgagttgt
     3901 tggaacgagt ctatccgatg ggcgaactcg ggcggtatct ggtggagcaa gttgtcgact
     3961 tcaacgctcg gcatgtgtcc ggccccatcg agcaccgttc ccttggagac tctcccgccg
     4021 tcggtgtcgt gatgtatcca gactgcggac gatccgagtg gcggcccgcg cccgagttcg
     4081 acgagtccat gcactacgtt cacactggcc agcatcgacc gatccgggtg tacgagacgg
     4141 ttgacgcgcg attcgtactg gaagacttct acgccaaact caagcggttt gcgaaaggtg
     4201 aaacggacgt gcccgagcac attgcctcac tcgcatcgca atgatgtcgc agacgtcttg
     4261 cacatggcgc acccgtcgcc tacgccgcct ttcagtctcc tga
//
LOCUS       DASYWM010000171        11609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_25187, whole genome shotgun sequence.
ACCESSION   DASYWM010000171 DASYWM010000000
VERSION     DASYWM010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25187"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..2494)
                     /locus_tag="VGR29_07050"
     CDS             complement(308..2494)
                     /locus_tag="VGR29_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073384.1"
                     /translation="MMRNRRGAPLLLVLMLVVGMIGAGQNVLAFAQNASIQPAGESGG
                     GDEIVVNGARYLRDREVTVNPNPLQQVADENGNSVYVKPGGSDPLGAVYVLAPEGGDR
                     AVRYLPEFVGDPAAACPSDLVARETIQGNGATYVAAGPETDMNADQLTEIGTSDDGAI
                     IYAYSPEQPFEEILVAQSQGQGNVPLTRYVMTGPDGVPAPFSDGLAFAGQQFAPVPGA
                     VTSIEGLTKVGCAGLFPAYSQAQEQPFSTITLQVGDQAVSFQAPEGDAGITPVESTPP
                     PALPTEVVATPPPPTEVVATPPPPTEVPPTATLAPEPTATATPEPTSTATPEPTATST
                     PEPTTTATPEPTATATAEPTATATPEPTVTATAEPTATLTPEPTATATPEPTPTATAE
                     PTATATSEPTATATPEPTTAPTQAPTAVPTDAVAQPAEGEEPTALPTEVPPTTAPGTV
                     EPTLPASRPTPIPQPLQPRAVVPTLPAGAPSPAASDRTTDVTCSGETGALDADGVPDR
                     LPTSIQYGGTGYRYTGQIAVADAGSVEAVGCVGGFVIFEPAEEADAGPIYLALDESPE
                     TLFRFEQARSFTVQSQVADQPRTIDTAGTEQQPAVRYRAAEPLVRSTYSSLSLIVYVA
                     DADIPAPERLLGYAVGGDVFAEYMLEGTGEQASQEVLAEAETFGVLGEMLLGGQRYVL
                     TSLWTPYGTTTNGWVTLYGPAGEDAPEQLVGLDPRREDLMVFNRTE"
     gene            2723..3928
                     /locus_tag="VGR29_07055"
     CDS             2723..3928
                     /locus_tag="VGR29_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2073385.1"
                     /translation="MRVFPGPRVSLIRLVTFLLMLVVASPTLAVAQDSTPSPNQASAR
                     TVNVELVLDSSGSMAQEVSPGVTRIQAAKEVLNDVVDAIPEREGVNVGFRVFGHEGSN
                     QEQDREESCRSTELIVPIEGVNKDALRGAIEGYTPVGWTPITLALQEAAEDFQAPQEG
                     EVNAVVLVTDGLETCGGNPCEAAEALAQGDVNVTTYVIGFGLTQEEQGTLQCIADAGG
                     GLNLGAANAEELNRALFTVLEELQVVIQTGFLEIEEFGGIYPKATVDCREGATDSNPE
                     GEVTSVTLTDSNRVELPVGSCEAYWVNPSGSESRVRIAIQPGETTWLRGSILKFPQGA
                     GEIYTVKDLSGTIIWQDQFEQGDYVWVLPGIYRMELLERVGDPILIMAEVQTLPGTVT
                     TLEIFTAGS"
     gene            4121..4681
                     /locus_tag="VGR29_07060"
     CDS             4121..4681
                     /locus_tag="VGR29_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073386.1"
                     /translation="MRPHTSFARSLAIGAFALVPGLLMSGAAVAQEGTPIATPGGPSE
                     GYPVAIHQGSCADLTAEAAFEIGDAITFGLEEDEAEGEVQTVGTGEALPLLLQASGTV
                     DSSLEDLGSQGHAVAVHASQDEYETIVACGDIAGINNGGKLAIALAPVDGSSVVGVAI
                     LEGEDQLEATVYVFDTQNAGGPTPEA"
     gene            complement(5012..5581)
                     /locus_tag="VGR29_07065"
     CDS             complement(5012..5581)
                     /locus_tag="VGR29_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073387.1"
                     /translation="MGQSSDQIRQEIDQHREDAAQKIDQLQSQVQGTGEDLRHQAEET
                     VDQVREQVAGSVEDTIQSVKENVNIQEQIEQRPLVALGAALIGGFVLGGMLSGGGGGQ
                     HRSSSSSHDYSGSSSSQGGGMGDTVRQAFKSSGLEETFSNAAAAMMGSVTEQVKQTID
                     RNVPGFSQKMESAKQQPGSVMDKTRKTQP"
     gene            complement(5545..6099)
                     /locus_tag="VGR29_07070"
     CDS             complement(5545..6099)
                     /locus_tag="VGR29_07070"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV2073388.1"
                     /translation="MDNTRNSRPGAGDERFEADSVRARPGGTPPDPVPVSPSRSSSTS
                     PIDEAKDIAKGTAESLGTIVSGIVEDLQQIVRGEVQLAKTELKEDATQIGKGAGMVGA
                     GVFFGLVGFIFLMLSLTYLLSEWMEPWIAAGIVGLGLVIIAAILAMSGKSQIQEANLK
                     PEETIDSLKEDQEWANRQIKSVKK"
     gene            complement(6128..6661)
                     /locus_tag="VGR29_07075"
     CDS             complement(6128..6661)
                     /locus_tag="VGR29_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073389.1"
                     /translation="MTYRDPLYDKDSPVEGENIDDLLHENSGQQRGGGQSQQKTEQVK
                     QKGQEVADQAKGKADEVTSKAKSKADEVGSKAQSKADEMGSMATDKADQGMDSAASGL
                     STAADKLRQQGEQQGGKAATAATTVADKLEGASGYLREKDTDQVLTDLEDLVRRKPVE
                     SLLAAAGIGLLLSRIFS"
     gene            complement(6840..8429)
                     /locus_tag="VGR29_07080"
     CDS             complement(6840..8429)
                     /locus_tag="VGR29_07080"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEV2073390.1"
                     /translation="MRTPKWLAVFFSFMLVFTGFAAVTAQESTPAAGEETEGSAWSDG
                     AFPGGTPAAQLGAPAPDQVAQPGEGQLSGDPQIQLVRVSDGLFEPINVTHANDGTGRV
                     FVVERAGRIRIIDGEGDLVEEPFLDISDNVQWQFLEQGLLGLAFHPDFAENGLFYVNY
                     TDLLRAGDLLTVQYQVSADDPNKADPDSGAIVWWRPNPYANHLGGEIAFGPDGYLYIG
                     HGDGGMEGDPLNAGQRLDTYLGKILRIDVSPAVQHAQAGAAGAEPGASMTALGGTGLY
                     AIPPDNPFTEGDVLIDLFRASEEDFAEYHPTARPEVWDYGLRNPWQFSFDRQTGDLWI
                     GDVGQNYWEEVDYGPAGYGGGVNWGWKFLQGSHCFPMSLESCPLVGTLPVAEYEHAAE
                     KSCTIVGGHVYRGEDFQNLDGIYFHSDYCSGKIWGIAAGEDGNWAYQELLDTGLLVTG
                     AGEDETGNIYFTSCECGYGQNAPNQAGSLWMLVASDQIPEGAETAPPDRDAAAAAEGT
                     PEATPAVGEGISTPEESTPEG"
     gene            complement(8767..9534)
                     /locus_tag="VGR29_07085"
     CDS             complement(8767..9534)
                     /locus_tag="VGR29_07085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2073391.1"
                     /translation="MNVQFEPVPIEDIRQEIGAHIAALPGPVDSFYEDHVLLSNHARI
                     VIGGQYAGHAAIHDESLITFFSLKPGFLAYGEAVFNALRRREQVTSTYIPTCDEFFLV
                     HALDDYRKLTPQARLFRHDHARMPMMVPDNLTLRPASHSDAELLGEASSFFDRIDQRI
                     EAGELFVLERDGERAGFGIREMSRLVPGRASIGMFTREEQRQQGVGSAIVALLIQRCR
                     EEGVEPVSGCWYYNHRSRRALERAGMNSTTRLLKFEF"
     gene            9681..9908
                     /locus_tag="VGR29_07090"
     CDS             9681..9908
                     /locus_tag="VGR29_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2073392.1"
                     /translation="MDTGSSHEEHSESDHIETLLHRDVYSPEEAAEVSGIPLRTIRSA
                     AFRGDLKAVIVGHDIISIERGDLLAWLQDRD"
     gene            10574..>11609
                     /locus_tag="VGR29_07095"
     CDS             10574..>11609
                     /locus_tag="VGR29_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073393.1"
                     /translation="MRSCRAPAASPSTTGVWGSVCCRQGGYFSQIHRWDLKTKERTVV
                     IDKLPDGGWHEPGGPIFGPDGLMYFAQGSVSLQGVVLPYGFTVDLAKHPNAMDVPGQD
                     VTLTGNNVWSRDPRSPFPFYVATGPYKPYGVPSHKGEVIKGELWCNTGVWRSQPDGSG
                     PELLAWVIRNPFGLAINEQGELYASDNDFEEKGDRAIAEDPDGIWHIRNASQPYGSIE
                     TPDWYGFPDICKDGLPAWDKKHLPNRSIPAEPLIEDPTPWAGPAAFLEKPHSSMAKMD
                     FSPSDAFGFRGELFACKWGTMAPLNTNRTEALDRGFKVIRLDTTTGQAETFLQNKQPA
                     PASYWTGSGGI"
BASE COUNT         2244 a         3526 c         3431 g         2408 t
ORIGIN      
        1 ggctacctgc gttgagagct gttcggcaac ttcgtcgcgt agggagtcct ggagacgcgg
       61 cagccccacg aagaatgcca gtacgcaaca cagggccaga agaccaatca caccggtcaa
      121 tccccacaga cagcctcgtc gacgcattcc atctaccttt cacctaaacg ctccgatttc
      181 aggcgtagca caagcccagg cattcgcagg ccttagcgct tacggaacgt agactggccg
      241 atattgagcg actggttgct caatatcggc cagtctgtca cgctgttctg ccccctgtgg
      301 agtcgtgcta ttcggtgcgg ttgaagacca tcaggtcttc ccgtcttggg tcaaggccta
      361 ccaactgctc cggggcatcc tcacccgccg gcccgtacaa tgtcacccat ccatttgtgg
      421 tggtgccata gggagtccag agcgaggtca gtacgtagcg ctgaccacca agtagcatct
      481 caccgagaac accgaacgtc tctgcctctg ccagcacctc ctgggacgcc tgttcgccgg
      541 taccttccag catgtactcg gcaaacacat cgcctccgac tgcatacccc aggagacgct
      601 ctggggcggg aatatcggca tcggccacat agacgatgag gctaaggctc gagtaggtag
      661 agcgtacgag cggctcagct gcccggtaac ggaccgccgg ttgctgctcc gtgccagccg
      721 tgtcgatcgt ccggggctgg tctgccactt gggattgcac cgtgaaggac ctcgcctgct
      781 cgaacctgaa gagtgtctcc ggtgactcgt cgagagcgag atagattggt cccgcatcgg
      841 cttcctcggc cggctcaaaa attacgaatc cacccacgca cccgacagcc tcgacggatc
      901 cggcatcggc aaccgcgatc tgtccagtgt aacgataacc agtaccgccg tattgaatgc
      961 ttgttggcag gcgatcggga actccatctg catccagtgc gccggtttca cccgaacagg
     1021 tgacgtctgt cgtgcggtca gaggcagcgg gcgaaggtgc acctgcaggc agtgtcggca
     1081 cgacggcgcg tggctggagt ggctgcggaa ttggggtcgg ccgcgacgct ggcagtgtcg
     1141 gctcgacagt accgggggca gtggttggcg gaacctcggt aggcagcgca gttggctcct
     1201 caccttccgc tggttgagcg acggcatcgg tgggcaccgc ggttggcgcc tgcgtcggag
     1261 cagtcgttgg ctccggcgtc gccgttgcgg tcggttctga tgtggcggtt gctgtcggtt
     1321 ccgcagttgc cgttggcgtt ggttctggtg tcgctgttgc ggttggttcc ggcgtcaatg
     1381 tcgccgtcgg ctctgccgtc gcagtcacgg ttggttcggg tgttgctgtc gctgtcggct
     1441 ctgccgtcgc agtcgcagtc ggttcgggtg ttgctgtcgt cgtcggctct ggcgttgagg
     1501 tcgccgttgg ttccggggtt gctgttgatg tcggctctgg ggtcgctgtg gcagtcggtt
     1561 caggcgccag tgtcgctgta ggaggaactt ccgtaggcgg tggaggagtg gcaacaacct
     1621 ccgttggtgg aggtggtgtg gcaacgacct cggtaggaag cgcgggaggc ggggtgctct
     1681 ccactggtgt gatccctgca tccccttcgg gcgcctggaa tgacacagcc tggtcgccga
     1741 cctggagcgt gatcgtcgag aagggttgtt cctgggcctg ggagtaggcc gggaagagcc
     1801 cggcacatcc gacctttgtt aggccctcaa tcgaggtgac ggcgcccggc actggagcga
     1861 actgctgccc cgcaaacgcc aatccgtcag agaacggtgc cggaacacca tccggcccgg
     1921 tcatcacata ccgcgtcagc ggaacgttgc cctggccctg actttgagct accaggatct
     1981 cctcgaacgg ctgctctgga ctgtaggcat aaatgattgc gccatcatca ctggtgccga
     2041 tttcggtcag ttggtccgcg ttcatgtccg tttccggacc ggcggcgacg taggtggcgc
     2101 cgttcccctg gatcgtttct cgcgcaacga ggtcagatgg gcaggccgcc gcagggtctc
     2161 cgacaaattc gggaagatac cgcacagccc gatcgccccc ttcaggcgca agcacatata
     2221 ccgcacccag tggatcgctt cctccgggct tcacgtagac tgaattgccg ttctcatcgg
     2281 caacctgttg taaaggattc gggttaacgg tgacttcgcg gtcccgaagg taccgggccc
     2341 cgttgactac gatctcgtca ccgcctccgc tctcacctgc tggctggatg gaggcatttt
     2401 gcgcgaaggc caacacattt tgtccggcac cgatcatccc gacgacaagc atcaggacca
     2461 gcaagagcgg cgcaccacga cgattccgca tcatctttcc tccggtatat tcggcatgga
     2521 tcaagcaaga atcaatatcg cgaaatatgc gcaacaatca ggccacccaa atcgtccagc
     2581 ctagcgacga caatggcata tttcgtgcaa gctccgccca gcagattctt aggacaatgg
     2641 agtggaatcg ctcgaaagcg attcctcact tcctgcctgt cggacacagg cgtacatgaa
     2701 gttagaagag aggaagagaa cgatgcgtgt ctttcccggg ccacgagtct cgctcattcg
     2761 acttgtcacg tttctgctga tgcttgttgt ggcgagcccg acactcgccg tcgctcagga
     2821 ttcgacacca agcccaaatc aggcaagcgc gagaacggtc aacgtcgagc tggttctcga
     2881 ctcctcaggc tctatggcgc aggaagtctc gcccggcgtg acacgtattc aggcagccaa
     2941 ggaggtcttg aatgacgtgg tggacgccat tccggagcgc gaaggcgtca atgtcggctt
     3001 ccgtgtcttt ggacatgagg gaagcaatca ggagcaggat cgcgaggaga gctgtcgttc
     3061 cacggagctg atcgtaccca tcgaaggtgt taacaaggac gcgctacgtg gtgcaatcga
     3121 aggctatacc cccgttggat ggactcctat cacacttgca cttcaggagg ccgcggagga
     3181 ttttcaggca cctcaggaag gcgaggtcaa tgccgtagtg ctggtgactg atggcctcga
     3241 aacctgtggc gggaacccgt gtgaagccgc ggaagcactt gcccagggcg atgtcaacgt
     3301 cacgacatac gtgatcggct ttggtctcac acaggaagaa cagggaacgc tccagtgcat
     3361 cgccgacgct ggtggcgggc tcaacctcgg tgccgccaat gccgaggaac tgaacagggc
     3421 gctctttacc gttcttgaag agcttcaggt agtcatccag acaggattcc tcgagatcga
     3481 ggagtttggc ggcatctatc cgaaggcaac cgtcgactgc cgtgagggag ccaccgactc
     3541 caatcctgag ggtgaagtta cctctgtcac cctgactgac tcgaacaggg tggagcttcc
     3601 ggtcggatcc tgtgaggcct attgggtaaa tccgagcggg tccgagtccc gcgtcaggat
     3661 tgcgatccag cccggtgaaa ccacctggct tcggggctcg atcctgaagt tcccccaagg
     3721 cgccggtgaa atttacaccg taaaggatct ctcgggcacc atcatctggc aggaccagtt
     3781 cgagcagggt gactacgttt gggtgcttcc gggcatctac cgcatggagt tgcttgagcg
     3841 cgtcggcgat ccaattctga tcatggcgga ggtccagacg ctgccgggta ccgtgacaac
     3901 gctggagatt ttcacagccg gctcctgacc taacgaactc tgcaacagcg agcggctgcg
     3961 gtgagttcac cgcagtcgct cgccaagttc catgaccctt atcaccatgc cgttgtggtg
     4021 gaatccggtt tgcgtaggaa gcatggcttg agggtaactg tgctactcgg cgcgtgatcg
     4081 ggtgagcatg ccgtctttat gcagaaggag tatcaggatc atgcgaccac atacgtcttt
     4141 cgcccggtct ctcgcaatcg gcgcatttgc cctggtgcca gggctgctta tgagcggagc
     4201 ggctgttgcc caggaaggga ccccaattgc cactccaggc ggaccctccg agggctaccc
     4261 ggtagcgatc catcaaggca gttgcgctga cctcactgct gaggcggcct tcgagatcgg
     4321 cgatgcaata accttcggcc tggaagaaga tgaagccgaa ggcgaggttc agacagtggg
     4381 tacaggcgag gcccttccgc ttctgctcca ggcttcgggc accgtcgata gctcgcttga
     4441 ggatctcggg agccagggac atgccgttgc ggtgcatgcc agccaggacg aatacgagac
     4501 aattgttgcg tgtggcgata tcgccggcat caacaatggg ggcaagcttg caattgccct
     4561 cgccccagtc gatggctcat ccgtagtagg cgttgctatt ctcgagggcg aggatcagct
     4621 ggaagccacg gtttatgtct ttgacaccca aaatgctggt ggtccaacgc cagaggccta
     4681 acgatgcagg tctaactcat cagcgcatag ggaagggccg gttgcatctg cgaccggccc
     4741 ttccctatgt gtccgctgcc tatgatccct gaaacacgta caggcctcat aggtgggacg
     4801 tcggcgatgt acgccatctc gtttgggatt actctaccgg cacatcctcg gcatacgagc
     4861 aggagtgctt tgttgtgacc tcccgcctcc cctaagtagg tgtgagctcg atgggcatac
     4921 acgaactgct cccgggagcg gttcccttac gccaaggagg aggtaccttt cggtacctcc
     4981 ttcatcgggt ccgatgttcg tagggctgtg gctacggctg tgttttccgc gtcttgtcca
     5041 tcacggaacc tggctgctgc ttcgctgatt ccatcttttg cgagaagccc gggacgttgc
     5101 ggtcaatagt ctgcttgacc tgttccgtga cgcttcccat catggcagct gcggcgttgg
     5161 agaaggtctc ttccaggccc gagctcttga aggcttggcg aactgtatcg cccattccgc
     5221 ccccctgcga ggaggaactg ccggagtagt catgggacga tgagctggag cggtgctgac
     5281 cgccgcctcc accgctgagc atcccaccga ggacgaatcc cccgatcagc gcggcgccaa
     5341 gcgccacaag cggtcgctgc tcgatctgct cctggatatt gacgttttcc tttaccgact
     5401 ggattgtgtc ttccacggat cccgctacct gctcgcggac ctggtcgacc gtttcctccg
     5461 cctggtggcg aagatcctcg cctgtgccct gaacctggga ttgcagctga tcaatcttct
     5521 gcgctgcatc ctcacggtgc tgatctattt cttgacggat ttgatctgac gattggccca
     5581 ttcctggtcc tccttaagtg aatcgattgt ttcttcgggc ttgaggttgg cttcctggat
     5641 ctggctcttg cctgacatcg caagaatggc cgcgatgatc accaggccca gcccgacgat
     5701 tccagcggca atccatggct ccatccactc gctgagcaaa taggtcaacg acaacatcag
     5761 gaagatgaaa ccgacgaggc cgaagaagac tccggcaccc accattccgg cgcccttgcc
     5821 gatttgtgtt gcatcttcct tcagctccgt cttcgccagc tgcacctcac cacgaacgat
     5881 ttgctgcaga tcctccacga tgcccgagac aatggtccca aggctctcgg cggttccctt
     5941 tgcaatatcc ttcgcctcgt cgatgggcga agtacttgag gagcgagacg gtgaaacggg
     6001 cactgggtct ggaggagtac cccctggtct ggccctcacc gaatcggcct cgaaccgctc
     6061 atcaccagct ccaggccgag aattacgtgt gttgtccata agagagttcc ttcgcgccgg
     6121 cactggccta gctgaatatt cgcgagagaa gcaggccgat accggcggca gccaacagcg
     6181 attcgactgg cttgcggcgg accagatctt cgaggtccgt cagcacctga tcggtatcct
     6241 tctcgcgaag gtagccactg gcaccctcga gtttgtcggc gacagtcgtc gccgccgtag
     6301 cggccttgcc accctgctgc tcgccctgtt gacggagctt gtccgcggca gtgctgagcc
     6361 cggaggcggc agagtccata ccctgatcag ccttgtccgt ggccatcgag cccatctcat
     6421 ccgccttgga ttgagccttg gagcccacct catcggcctt gctctttgcc ttggaagtga
     6481 cctcgtcggc cttgcccttg gcctggtccg cgacttcctg gcccttctgc tttacctgct
     6541 cggttttctg ctgagactgg ccgccgccgc gctgctggcc gctgttctca tggagcagat
     6601 catcgatgtt ttccccttcc actggggaat ccttgtcata cagcggatct cgatacgtca
     6661 tctaaagcct cctcgggagt gtttcacgca caaaagacct ccggtttcgg agattggtag
     6721 tgcccgacat gcaacatgcg tgccatgttg ctcctgcgac atctccttag gagaaaagcg
     6781 gcggggacac caatgtcccc accgcttttc ttttcggcat tcggctaatg ccaggggcgc
     6841 tagccttccg gcgtgctctc ctctggtgta gagatgcctt cgcctaccgc cggcgttgct
     6901 tccggcgtgc cctctgcggc agccgccgcg tcccggtcag gcggggctgt ctccgcgccc
     6961 tcgggaatct ggtcggacgc gacgagcatc cagagcgatc cggcctggtt gggcgcgttc
     7021 tggccgtatc cacactcaca ggaagtgaag tagatgttac cggtctcgtc ctcgccagca
     7081 cctgtcacga gcaaccccgt gtcgagcagt tcctggtatg cccagttgcc atcctctccg
     7141 gctgcaatac cccagatctt gcccgaacag tagtcggaat ggaagtagat gccatccagg
     7201 ttctggaaat cctcgccgcg gtatacatga ccgccaacga tcgtgcagct cttctccgcc
     7261 gcgtgctcgt actcagccac aggaagtgtc ccgacaagcg ggcaggactc cagtgacatc
     7321 gggaagcaat gggaaccctg gaggaacttc cagccccagt tcacgccgcc cccgtacccg
     7381 gcgggcccgt agtcaacctc ttcccaatag ttttgtccca cgtcaccaat ccacaggtcg
     7441 cccgtttgcc ggtcgaagct gaactgccac ggattgcgta gtccgtagtc ccagacctcc
     7501 ggtcgcgcgg tcggatggta ctcggcgaag tcttcttctg acgcgcggaa gagatcgatc
     7561 aggacgtcgc cctcagtaaa ggggttatcg ggcgggatag cataaagccc ggtaccgcca
     7621 agcgcggtca tcgaggcgcc cggctcggca cccgccgctc ctgcctgcgc gtgctgaaca
     7681 gccgggctca cgtcaatgcg gagaatcttg ccgagatagg tgtcgaggcg ttggccggcg
     7741 ttcagtgggt cgccctccat gcccccgtca ccgtgaccga tgtagaggta tccgtcaggg
     7801 ccaaatgcga tctcaccgcc aagatgattg gcatacgggt tgggtctcca ccagacgatc
     7861 gcgccgctat ccggatctgc cttgttcgga tcgtcggctg aaacctggta ctgcaccgtg
     7921 agcaagtccc cggcacgcag aaggtctgtg tagttaacat agaacagccc gttctccgca
     7981 aaatctggat ggaaggccag gcccagcagg ccctgctcga ggaactgcca ttggacgttg
     8041 tccgagatat caaggaacgg ctcctcgacc aggtcccctt ccccatcgat aatccggatc
     8101 cgccctgccc gctcaaccac gaagacacgc cctgttccgt cattcgcgtg agtgacgttt
     8161 atcggctcga acagcccgtc tgagaccctt acgagctgaa tctggggatc gccggagagc
     8221 tggccctcgc caggctgagc gacctgatcc ggtgctggcg cacctagctg tgctgctggg
     8281 gttccgccgg ggaacgcacc atcgctccag gcggagccct ccgtctcctc cccggctgca
     8341 ggtgtggact cttgagccgt gaccgcggca aaaccagtga acaccaacat gaatgaaaag
     8401 aaaacagcca accactttgg tgtgcgcatg ctgctcactc cttgatgtat ggatgcccca
     8461 accgctattt attgggtgcg gggcgcaccg cacgatgcgt tccatgggac gaggcgaccg
     8521 ggtacgttgt tgggtttgtt gggtaaagac gttgcatcca ctgccggcgc ggacgattgc
     8581 ccctcatcga ggatgcagac acagacagtc tggcaaaggc gctcgaaatg tccggcggat
     8641 tattcggtcg atttttcaca cgctcgaccg ccatattgcc gcaagaggcg ggacctcctt
     8701 gtgggaggac ccaatgtcgg tagactacga cctcatcacc gtccatgaag ttcggtgaga
     8761 cgtggatcag aactcaaact tcaacagacg tgtggtggag ttcatccccg cccgctccag
     8821 cgcacgccgg gaccgatggt tgtaatacca gcatccggaa accggctcaa cgccctcctc
     8881 ccggcaacgc tggatgagca acgccactat tgcgctgcct acgccctgct gccgctgctc
     8941 ttcccgcgtg aacatgccga tactcgccct gccgggtaca aggcggctca tctcgcggat
     9001 gccgaatcct gcacgttcac cgtcccgttc cagtacgaag agctcgccgg cctcgattcg
     9061 ctggtcaata cggtcgaaga acgaggacgc ctctccgagc agttctgcat cgctgtgact
     9121 ggccgggcgc agcgtcaggt tgtccggcac catcatgggc atccttgcat gatcgtgcct
     9181 gaagagacgg gcctgtggcg tcagctttcg gtagtcgtca agcgcatgca ccaaaaagaa
     9241 ctcgtcgcaa gtaggaatgt aggtggaggt gacctgctca cgacgccgga gtgcattgaa
     9301 gacagcttcc ccataagcaa gaaaccccgg cttcagtgag aaaaaggtga tcaacgattc
     9361 gtcgtggatc gccgcatgcc ccgcatactg gccaccaatg acaatccggg catggttcga
     9421 taacaagacg tggtcctcgt aaaagctgtc cactggacct ggaagcgctg cgatgtgcgc
     9481 tcctatttcc tgccgtatat cctcaatcgg caccggttcg aactgcacat tcatgtctga
     9541 ctcctgtcga ttgccctgcc actcgtaatg tcaaaaggcc gcctgtttcg tcggccgggt
     9601 tggggagcat ccatgcaggg gctattcgct gtaatatgaa cgaagatgtc gtcagaacga
     9661 attggatgga aggacgttgc atggatactg gctcttccca tgaggaacac tccgagagcg
     9721 atcatataga aactctgctg cacagggatg tctattctcc cgaggaggca gcggaggtca
     9781 gcgggatccc gctgcgaaca atcaggtcgg cagccttccg gggagatctg aaggctgtca
     9841 tcgtaggcca tgacatcatc agtatcgagc ggggagacct gctcgcatgg ctccaagatc
     9901 gcgattgact tttgcctcgg gtgcccgaga cgcgcggctc cgcaacatcc ccttgcatga
     9961 ctctcgcaac ccgtcgagcg atcccttacg acgctcaggg agccaaaggc gggggcgcct
    10021 atagtctccc agctcgcgag gtcgtgcgcg acctcccgct cgtgactgat tgataccccg
    10081 cagaacaatc tgcgtcgtct acctgaacga ccgctcccaa agatgaattc tgctccccgt
    10141 ctcgcgcccg gcagccggta actttttgga acacgccttg cgggccctca atgccatatc
    10201 gggctgccaa tccaacccaa gcctatggag gcttaggtag caccacctgt aatcgtccgg
    10261 atagcaggaa ggcagcgaaa ggcgaagccg gaggagttct gaatgcctat tcatgccgtt
    10321 cagggatcca gagaggcgct ccagcacctc cccaccaggg cccgtccacg agtagacccc
    10381 agcgacgtcc ctgtgccgga gggatacagc attgagccgg tactcgtcgg gctcacattc
    10441 cccaacgatt tctgctttga cagggatggc acgatctgca ttggcgaggg aggaagcaca
    10501 tggcccaccc gtccagcacc gccgccgcgc atcctcaagt ttgacccttt aggcaacctg
    10561 gaggagttcg tcagtgagat catgccgggc ccccgcggcc tcgccatcca cgacgggggt
    10621 ctgggggtct gtatgttgcc gtcaaggggg ttatttcagc cagatccacc gctgggacct
    10681 caagaccaaa gagagaacgg tcgtcatcga caagttgccg gatggcggct ggcatgagcc
    10741 gggtggtccg atattcgggc ccgacgggct catgtacttc gcccagggct cggtgagtct
    10801 tcaaggtgtg gtgctcccat atgggttcac cgtcgacctc gcaaaacacc cgaatgccat
    10861 ggatgtgccg ggacaggatg tcacgctcac tggaaataat gtctggagcc gtgaccctcg
    10921 ctctcccttt cccttctacg ttgctactgg cccctacaag ccgtatggcg tgccatcaca
    10981 taagggcgaa gtcatcaagg gagagttgtg gtgcaacaca ggagtgtggc gttcgcagcc
    11041 ggatggaagc ggaccagagc tgttggcgtg ggtgatccgg aacccgttcg gcctggcgat
    11101 caacgagcag ggtgagctat acgcatcgga caatgatttt gaggagaagg gcgaccgcgc
    11161 gatcgccgaa gatcccgacg gcatctggca catccgaaat gccagccagc cctatggctc
    11221 catcgagacg cccgattggt acggcttccc ggacatctgc aaggatggac tacctgcgtg
    11281 ggacaagaag catctgccaa atagaagcat cccggctgag cccctgatcg aggatccgac
    11341 cccttgggct ggacctgccg cattcctgga gaagccccat agctcaatgg cgaaaatgga
    11401 cttctcacca tccgatgcct tcggtttcag gggcgaactc tttgcctgca aatggggcac
    11461 catggcgcca ctgaacacca atcgcaccga agcattggac cgtggcttca aggtcatccg
    11521 cctcgatacg acgacgggcc aggcggaaac gttcctgcag aacaagcagc cagcgccggc
    11581 ttcctactgg actggaagcg gcggtatcg
//
LOCUS       DASYWM010000172         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_401425, whole genome shotgun sequence.
ACCESSION   DASYWM010000172 DASYWM010000000
VERSION     DASYWM010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401425"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(453..2501)
                     /locus_tag="VGR29_07100"
     CDS             complement(453..2501)
                     /locus_tag="VGR29_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEV2073394.1"
                     /translation="MPNRLANETSPYLRQHKDNPVDWYPWGPAAFEEAKRRDVPVLVS
                     IGYSSCHWCHVMAHESFEDPDMAKAMNDRFVNIKVDREERPDVDAIYMQATQAMGNQG
                     GWPLNVFVTPDGLPFFGGTYWPKYDHPPMPGFQRVLTTIAAKWREDRENLLLGGQKVA
                     DYLRQSAEATPPRSPVTPELLTQAVDALKQQFDPEWGGFGNAPKFPQPSTLRFLLRHY
                     YRTKDLRALRMAEMTLDMMAAGGIHDQLGGGFSRYSVDERWHVPHFEKMLYDNAQLLD
                     LYTELWTITKDDLYRDVASGIVAWLNREMLTEGGGFAAALDADSEGVEGKYYIWSAAE
                     VDQLLSADAADLVKLHFGITDPGTFEGKTVLSVVRSVEEIAEQTGQDIDAITSTLSEA
                     KARMLEVRQQRVAPGRDDKVIAGWNGLMIHALAHAGIVFQQPDWVSMAGKAADFILQH
                     LQNEDRSIARSWNSGQTRGDGVLEDYACMAYGLVELYAATGNRNWLNHGSDLLDYVRK
                     HFRHDSGIGFYDTSDTAENLITRPRDLTDTAVPSGNGVMAEMIFVFGIMEQKVEMIEA
                     SAGLIESLVRPMGDYPLFMGQFLSVAQRIVESPRELVFAGTPASERIATLRYAAGSQH
                     EPLLVVGYNDPGDPGAGKRFPMLTDRPVVDDGAAYLCQDFTCLPPATTGDKLVEILNS
                     "
     gene            complement(2694..>3174)
                     /locus_tag="VGR29_07105"
     CDS             complement(2694..>3174)
                     /locus_tag="VGR29_07105"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2073395.1"
                     /translation="MATAVTFALGLVGFLLLPTAPPWMSNPDDVTRVTRHILVETAGL
                     SAGSDQDAVMASAFRFEPNHLAAMPSIHVSVIVIVFLLLRRIGPKPGVMGAIYSLLMT
                     LGVVYLGEHFVLDAIFGWGSALTGWTLACRWIAEGKDNRILLNHQHHPAVPGSGSGY"
BASE COUNT          644 a          941 c          946 g          643 t
ORIGIN      
        1 gcgccacctc atcggtctgg ctgatccggc gaggacgacc agaccgaggg cgggcccggc
       61 aggagcccgt gcgggcacgc tgccggtacc agcggcgaag ggtggatgaa tcaatggcat
      121 acattgcggc gacgtcaacc actcgatgac cgtcatccac tgcggccagg gctcgctcac
      181 ggacgtcgtg agaggcgtgc aggcacatca tcccccacag tctgccagac aggcaggata
      241 tatgaaagcc gctttaatat gcgggcacgc gggcggggaa tcgatccaac agaaccgggt
      301 cggccatgcg cacaggcaag cgcgaacgaa gcatttggtc ggtggctgga ggaggcgacc
      361 cgtctcgaca taccgatcgt cgcctcacta ccctttgata ccttcgcgga ccgaatcctc
      421 aaggctatcc agtagtcttg gcaggacctg gattacgagt tgagaatctc aaccagcttg
      481 tctcccgttg tggccggtgg gaggcaggtg aagtcctggc aaagataggc ggcaccgtcg
      541 tcgaccaccg gacgatcagt cagcatcgga aaacgcttcc cggcacccgg gtcgcctggg
      601 tcgttgtaac caacgaccag gagcggctca tgttgcgagc ctgccgcata ccgcagcgtg
      661 gcaatgcgct cggacgcggg ggtgccggcg aagacaagct cccggggtga ctccacaatc
      721 cgttgggcta ccgagagaaa ctggcccatg aagaggggat agtctcccat cgggcgaacc
      781 agggactcga tcagtcctgc cgacgcctcg atcatctcca ccttctgctc catgatcccg
      841 aagacgaaga tcatctctgc catcacgccg ttgcctgagg ggactgccgt gtcggtgagg
      901 tcacgcggac gggtgatcag attctcggcg gtatccgacg tgtcgtagaa gccgatgcct
      961 gaatcatgac ggaaatgctt ccgcacatag tcgagcagat cactgccgtg gttcagccaa
     1021 ttacggtttc cggtcgcggc atagagctcc acgaggccgt acgccataca ggcatagtcc
     1081 tcgagcacac cgtcacctcg tgtctgcccc gagttccagc tgcgggcgat ggagcggtcc
     1141 tcattttgca ggtgctggag gatgaagtcc gcagccttcc ctgccatgct gacccagtcg
     1201 ggttgctgga acacaatccc ggcatgggcc agggcgtgga tcatcaggcc gttccagccg
     1261 gcgatgacct tgtcgtcacg accgggtgcc acacgctgct gccgaacctc aagcatccgg
     1321 gctttcgcct ccgaaagcgt tgaggtgatg gcgtcgatat cctgtcccgt ttgctcggcg
     1381 atctcttcga cggaccggac gactgaaagg acagtcttac cctcgaaggt gccggggtcc
     1441 gtgatcccga agtggagttt gacgaggtcc gcggcgtcag cggatagtaa ttgatccacc
     1501 tcagcggctg accatatgta atatttgccc tccacgcctt cgctgtcagc atcaagtgcc
     1561 gcggcgaacc cgcctccttc tgtcagcatc tctcggttga gccaggcgac gatgccgctg
     1621 gcgacatccc gatagagatc atccttcgtg atggtccata gctcggtata gagatctagc
     1681 aactgggcat tgtcgtagag catcttctcg aagtgcggga catgccaacg ctcatccacg
     1741 gaatatcggg aaaatcctcc gcccagctgg tcgtggatgc cgcccgctgc catcatgtcg
     1801 agcgtcatct cagccattct tagggcacgg agatccttcg tccgatagta atggcgcagg
     1861 aggaatcgca acgtcgatgg ttgtggaaat tttggagcgt tcccgaaacc gccccattcc
     1921 gggtcaaatt gctgcttaag cgcgtccacg gcctgggtca ggagctccgg cgtcactggg
     1981 ctgcgagggg gagtcgcctc cgctgattgc cggagatagt cggcgacctt ttgccctccc
     2041 agcaacaggt tttctcgatc ttctcgccac ttggcggcga tggttgtaag cacgcgctgg
     2101 aagccaggca tcgggggatg atcgtacttc ggccaatagg tgccgccgaa gaagggcaat
     2161 ccatccggcg taacgaagac gttcagtggc cagccgccct gatttcccat tgcctgggtc
     2221 gcctgcatat agatcgcgtc gacatccggc cgctcctcac ggtccacctt gatgttcacg
     2281 aagcggtcgt tcatggcttt cgccatgtcc ggatcctcga acgattcatg ggccatgaca
     2341 tggcaccaat ggcaggatga gtagccaatg ctcacgagca ccggaacatc tcgccgcttt
     2401 gcctcttcga acgctgcggg tccccatgga taccagtcga cgggattgtc cttatgttgg
     2461 cgcaggtagg ggctggtttc attagcaagt cgatttggca tggatagctc tttctattgc
     2521 atctattgtg ctggcgattg agtgaccggt gaggacaact atggattgcg acgctgactt
     2581 cctgctttgc cctctacacc agcattggga tgacaggaac ggtacaggaa tgcggcgact
     2641 gcacgcgtca ctttcagatc agtgactgca cttaccttac tacggcgtta ttttcagtag
     2701 ccgctaccgc taccggggac cgcggggtga tgctgatgat tgagcaggat gcgattatcc
     2761 ttcccctccg ctatccatcg gcaagcaagc gtccaacccg tgagcgcact gccccagccg
     2821 aaaatcgcat caaggacaaa gtgctccccc agatagacga cgcccagggt catgagaagt
     2881 gagtagatcg ctcccatcac gccaggcttg ggacctattc gtctcaggag caggaacacg
     2941 atcacgatca ccgagacatg gatcgacggc attgctgcca aatgattggg ctcaaatcgg
     3001 aatgccgatg ccatcactgc gtcctgatcg cttcctgcgg atagtcctgc cgtttcgaca
     3061 aggatgtggc gcgtgactcg tgtcacatcg tccggattgg acatccacgg tggggctgtc
     3121 ggcaaaagca ggaagccgac caggccgagc gcgaacgtga cggcggtggc catg
//
LOCUS       DASYWM010000173         4736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_25334, whole genome shotgun sequence.
ACCESSION   DASYWM010000173 DASYWM010000000
VERSION     DASYWM010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25334"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..576)
                     /locus_tag="VGR29_07110"
     CDS             complement(88..576)
                     /locus_tag="VGR29_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV2073396.1"
                     /translation="MPGKSHHYPVTVTWTGNTGAGTARVNAYSKDHEVAANSKPVIPA
                     ASDPSFGGDPTRWNPEELLVASLSQCHMLWYLSRCARNHIVVTAYVDNAEGTMTEDAS
                     GGGCFDHVVLRPEITLADEKDQERADALHHEAHELCFIANSVNFPVTVEPVYGTAISE
                     SP"
     gene            complement(712..1104)
                     /locus_tag="VGR29_07115"
     CDS             complement(712..1104)
                     /locus_tag="VGR29_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073397.1"
                     /translation="MGVARIEHETGSFAVVMEGLDRIWTMRHRVTVPYQSVISVAWDV
                     HHAAAGPEGIRFPGTGFPGVITAGTYWRFWGEDRVRSFWLRGRDPRNCITVRLSDHAF
                     DYITVEVAHPEAEVTRLTAAVQEHQRGG"
     gene            1216..2760
                     /locus_tag="VGR29_07120"
     CDS             1216..2760
                     /locus_tag="VGR29_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2073398.1"
                     /translation="MRDRTDRSKAGEGRAPSYGWLAGAIAMLGMLAVLILGRSITGAV
                     SLVDALADAMLLAMPLSVFSWLLDLLGQQAKTFLLVGLVLLLILVGAWLGGCFVRRVI
                     AEPASIWTRASKLAILLFGFAAGFVLLFVNSQSPEVFSGGGFISTAIVLGLASVVFAL
                     ILAGLTLLFLRSKSGVESRGRRGAIVWGGVAVASLLGLIPVGREISRVAGRKTNLEVS
                     RGEMPPAITPIEDFYTISKNFVDPPNDRGPDWSIEIDGLVDQDLTLGRADLEELGRGD
                     FISTMLCISNTVGGGLIGTADWSGVSLRTVLEQAGIGKGAVKVIFEGTDGYTTGVPIE
                     RVMQPESHLVWGMNGQPLPREHGAPVRAIVPGLYGMKSVKWLTKMTVTEDDYQGYWEQ
                     RNWTDEAVVKTMSRIDLPINNEVLPAGPVKIGGVAFAGDRGVDAVEVSTDDGESWQRA
                     EIQAQPNPNGIAWVLWTHEWSAKPGAYTLAVRAIEGTGEVQTSEEASELPDGSSGWHR
                     ITVGVV"
     gene            complement(3078..3578)
                     /locus_tag="VGR29_07125"
     CDS             complement(3078..3578)
                     /locus_tag="VGR29_07125"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2073399.1"
                     /translation="MDLLDRMVGHDRWATTQLLELCSTLTDVQLDQEFDIGRRTLRET
                     LDHMIYVIDFWTGWMAGRPVDHDRTTQQYDRSITALIERHERYHANFAEFARRVHDEQ
                     RLDETFVDHNGVRQSLGATIIQLLHHNAQHRSEVRHMLERMGVAELWDYDPQEWEHVT
                     GRVQSV"
     gene            complement(3634..>4736)
                     /locus_tag="VGR29_07130"
     CDS             complement(3634..>4736)
                     /locus_tag="VGR29_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEV2073400.1"
                     /translation="QGDEFAHFLDLYAPDGKAPGIGDTWRNADKANTLRRIGETYAEA
                     FYIGEIGEKIVDFAQSTDGFITAEDLAQHTSTWVEPIGTNYRGYDVWEIPPNGQGIAA
                     LVALNILEGFDLASLERNSTESFHLQIEAMKLAFIDAHRYVGDPERVDVPVDGMLSKT
                     YAATRRGLIEDTALDPEPGAPPHSDTVYLCAADADGMMVSFIQSAYKGFGSHVVVPGT
                     GFSFQNRGSGFSLDPEHPNALEPGKRPFHTIIPGFLTKDGAPIGPFGVMGGFMQPQGH
                     VQMIVNTIDYDMDPQTSLDQPRWQWDKGRTVLLESTVDPAILDALLQCGHEAEVRENL
                     GAFGRGQIIWRLPSGALIGGSDHRADGQATGY"
BASE COUNT          867 a         1459 c         1487 g          923 t
ORIGIN      
        1 gaacctcgac cacggccggg tgggagctac cttttggcat tgtgatgcca atcgtgagga
       61 gaaggagtcg ctcacggtgt ggtcagacta cggggattcg gaaattgccg tcccgtagac
      121 cggctcgacg gtcaccggga agttcaccga attcgcgatg aagcagagct cgtgcgcctc
      181 gtgatgcagg gcgtccgcgc gctcctgatc cttctcgtcc gcaagcgtga tctccggccg
      241 gagcaccacg tgatcgaaac atccaccgcc gctggcgtct tccgtcatcg tgccctcggc
      301 attgtcgaca tatgccgtca cgacgatgtg gttcctggcg cagcggctga gataccagag
      361 catgtgacat tgcgagagcg acgcgaccag gagctcctcc gggttccagc gcgtcggatc
      421 tcccccgaag gagggatccg acgccgcagg gatgaccggc ttgctgtttg ccgccacctc
      481 gtgatccttt gaatacgcgt tcacacgggc ggtgccagca ccggtgttgc cggtccaggt
      541 aacggtcacg ggataatgat ggctcttgcc gggcatgcgg gctcctcgtg cgcgtggtat
      601 tacaagcgac taccggcagg ataccgccga tagcctgccg aatccggaaa tgggaaacgg
      661 ggaccactac caggaccggc agcagcgtag tgtgccgaac ccccgattcg gttacccgcc
      721 tcgctgatgc tcctgtacgg cggcggtaag acgggtaacc tcagcctcgg ggtgcgcaac
      781 ctccaccgtg atgtaatcaa aggcatgatc gctcagccgg actgtgatgc agttgcgcgg
      841 atcacgacca cgcagccaaa aggatcgaac tcgatcttct ccccagaacc tccagtacgt
      901 gcctgcggtg atgacccccg gaaacccggt tccggggaat cgaatcccct cgggcccggc
      961 cgccgcgtgg tggacatccc acgctacgga gatgacagat tggtacggca cggtgacccg
     1021 gtgccgcatc gtccagatcc ggtcaagacc ttccatgacg actgcgaatg atccggtttc
     1081 atgctcgatt cgggcaaccc ccacgagtcc tccttccttc attatccctt catcatggtg
     1141 cggtagagta cagtcatgtt gcgaccacat cttccatcat agatgacgac ccaccacaga
     1201 agaggcggcg atacgatgag ggaccgaacc gacagaagca aagccggtga agggcgcgca
     1261 ccctcttacg gatggcttgc cggggcaatc gcgatgctcg gcatgctcgc cgtgctgatt
     1321 cttggccgga gtatcaccgg ggcagtttcc ctggtcgatg cactggccga tgccatgctc
     1381 ctggcaatgc cactgagcgt cttctcatgg ctgctcgatc tcctgggcca gcaggcgaag
     1441 accttcctgc tcgtcggact ggtgctcctc ttgatcctgg tgggtgcatg gcttggcggt
     1501 tgcttcgtgc gcagggttat agcggaacca gcgagtatct ggacccgggc aagcaagctg
     1561 gcgatcctcc tctttggctt cgccgcgggc ttcgtcctgc tcttcgtgaa ctcgcagtcc
     1621 ccggaggtct tctccggtgg tgggttcatc tccaccgcca tcgtgctcgg cctcgcctcc
     1681 gtcgtcttcg cgttgattct tgccgggctc accctgctct tcctgcggtc caaatccggc
     1741 gtcgagagca ggggacgacg tggggccatc gtctggggcg gggttgcagt cgcatcgctg
     1801 ctcgggctga taccggttgg ccgggagatc agccgcgtcg cgggtcgcaa gacgaacctc
     1861 gaggtctcac gcggcgagat gccgccggcg atcaccccga tagaggattt ctacaccatc
     1921 tccaagaact tcgtggatcc ccccaacgat cgcggtccgg actggtcgat cgagattgat
     1981 ggtcttgtcg accaggacct gacgctggga cgggccgatc tggaggagtt gggccgcggc
     2041 gactttatct cgacgatgct ctgcatcagc aacaccgttg gcggcggcct gatcggcacg
     2101 gcggattgga gcggtgtgtc gttgcggacg gtgcttgagc aggcaggcat cggtaagggc
     2161 gcggtcaagg tgatcttcga gggtaccgac ggatacacca caggtgtgcc gatcgagcgg
     2221 gtgatgcagc cggagtccca tctcgtctgg gggatgaacg gccagccgct gccacgcgag
     2281 catggtgcgc cagtgcgcgc tattgttccc ggcctctatg gcatgaagag cgtcaaatgg
     2341 ctgacgaaga tgaccgtcac cgaggacgac taccagggct attgggagca gcgtaactgg
     2401 acggacgaag cggtggtcaa gaccatgagc cggatcgacc tgcccatcaa caatgaggtg
     2461 ttgccggccg ggccggtgaa gatcggcggt gtcgcctttg ccggagaccg cggggtagat
     2521 gcggtcgaag tctccaccga tgacggcgag tcctggcaga gggcagagat ccaggctcag
     2581 ccgaatccga acggcattgc ctgggtgctc tggacacacg agtggagcgc gaaaccgggc
     2641 gcctacacgc tcgccgtgcg cgcaattgag ggcaccggcg aggtgcagac atccgaggag
     2701 gcatccgagc tgccggacgg ttccagcggc tggcatcgga tcaccgtcgg ggtggtctag
     2761 cgtctcacca accctgttcg cccctgtgcc gcatccctac atcaggttgc acgaagattg
     2821 atgtcgcagt gcggccaggc aaagggcgta gaggacgatt aggtgggggt gtagcggacg
     2881 acgcctaggc aattgcggag caattgagcg acagcggccc ctttgggtga gcccttgggc
     2941 gaaaagcggt agcgaccctt tgccgcagca atgtcgtcct gtgtgcccta taaggaggcc
     3001 attgctacgt ccgtgcgcac taagaaagtg gttgcggttt ccatccccca agtctgtcgt
     3061 ggactctgga acgtccatca cacactctgg acgcgtccgg tgacatgttc ccactcctgg
     3121 ggatcgtagt cccacagctc ggctactccc atgcgttcga gcatatgccg cacctcgctg
     3181 cggtgctgcg cgttgtgatg gaggagctgg atgattgtgg cgccaagcga ctgccgaaca
     3241 ccattatggt caacgaacgt ctcatccagg cgctgctcgt catgcacgcg gcgagcgaac
     3301 tccgcgaagt tcgcgtggta gcgctcgtga cgttcaatga gggcggtgat cgatcgatca
     3361 tactgctgag tggtccggtc gtgatccact ggtcgcccgg ccatccaccc cgtccaaaag
     3421 tcaatgacgt agatcatatg gtcgagtgtc tcgcgcaggg tgcggcggcc gatgtcgaac
     3481 tcctgatcga gctgcacgtc ggtcagggta ctgcacagct ccagaagctg agtcgtcgcc
     3541 cagcgatcgt gtcccaccat ccgatcgagc aggtccatcc gctctccatc ccgttgagtc
     3601 ccttgcaggc atgcaacccc tttggaacca gcgttaatat ccggttgcct ggccgtcggc
     3661 ccggtggtca ctgccaccga tcagcgctcc tgatggcaga cgccagatga tctgcccgcg
     3721 cccaaacgcg cccaggttct cccgcacctc ggcctcatgg ccgcactgca ggagcgcgtc
     3781 caggattgcc ggatcgaccg tcgactccaa caagaccgtg cgccccttgt cccactgcca
     3841 gcgcggttgg tcgaggctgg tctgcgggtc catgtcgtag tcgatcgtgt tgacgatcat
     3901 ctggacatgt ccctgcggct gcatgaaccc gcccatcacc ccgaacggtc cgatcggcgc
     3961 gccatccttc gtcaggaaac ccggaataat cgtatggaag ggtcgcttcc ccggttcaag
     4021 cgcattcggg tgctccgggt cgagcgagaa gccgcttccc cggttctgga aggagaatcc
     4081 cgttcccggg acgacaacgt gggaaccgaa tcccttgtag gcggactgga taaagctcac
     4141 catcatgccg tcagcatcgg cggcgcagag atagaccgtg tcgctatgcg gtggcgcccc
     4201 cggctcgggg tcgagcgcgg tgtcttcgat cagcccccgg cgagtggccg catacgtctt
     4261 ggagagcatc ccgtctaccg gcacgtcaac ccgctccgga tcgccaacgt accgatgggc
     4321 atcaatgaat gcgagcttca tggcctcgat ctgaaggtgg aagctctcgg tcgagttccg
     4381 ctccagcgac gcgaggtcga aaccctccag gatattcagg gcaacaagtg ccgcgatacc
     4441 ctgcccgttg ggcggaatct cccagacatc gtaaccgcgg tagttggtgc cgattggctc
     4501 gacccaggtg ctcgtgtgct gggcgagatc ctcggcggtg atgaacccat cagtggattg
     4561 cgcgaaatcg acaatctttt cgccgatctc cccgatatag aacgcctcag cgtacgtttc
     4621 gccgatgcgc cgaagcgtgt tggccttgtc ggcattgcgc cacgtgtcgc caatcccggg
     4681 tgccttcccg tccggagcat agagatcgag aaaatgggcg aactcgtcgc cctgca
//
LOCUS       DASYWM010000174        12392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_7430, whole genome shotgun sequence.
ACCESSION   DASYWM010000174 DASYWM010000000
VERSION     DASYWM010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12392
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7430"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VGR29_07135"
     CDS             <1..560
                     /locus_tag="VGR29_07135"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEV2073401.1"
                     /translation="TFFRLIVALPILLLLGAVSGGAGDWSSDNMASAAGVAGVHFFAP
                     ILMIVFRQKYPRWWFDWNLELQRFSNRVTTYLALMDDRYPSTDEQQAVHLDYAYPDVR
                     RDLNRWLPLVKWFLAIPHVIVLVVLNIVAVVAVVMAWVAILVTGRYPRGLFNYVEGVF
                     RWNNRVIGYAVTLVTDTYPPFRLSE"
     gene            830..1315
                     /locus_tag="VGR29_07140"
     CDS             830..1315
                     /locus_tag="VGR29_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073402.1"
                     /translation="MKQIRALSWSLPASIRVVGVALIATIVLCAMDASEPVESHDTPA
                     STLRISVVHYLEAGGYTNAGQAEGQMCLPFGTVGPWETGGPQLIVTGNTGHIVGIVDV
                     MQGTFEQIDERIGCVVRAELPIVDASLFTFAVADVYNETVTRDALVAAGWVYEVEVDL
                     P"
     gene            complement(1459..1806)
                     /locus_tag="VGR29_07145"
     CDS             complement(1459..1806)
                     /locus_tag="VGR29_07145"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEV2073403.1"
                     /translation="MATNQETADDLVQQLQGSCPVTTKKMFGEYAVYHEGVVVALLCD
                     NQLFVKKTAPGLSFVQDPTGIPAYPNARNYLRVPRDRWIDVAWLSSFIKSTAEALRKT
                     RVPKVETRSGGSS"
     gene            complement(1966..2547)
                     /locus_tag="VGR29_07150"
     CDS             complement(1966..2547)
                     /locus_tag="VGR29_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073404.1"
                     /translation="MATNSEQGGEETVIRWTDSDGHEHVGRAHRDQDKPLDLETLEAA
                     GVDIEEVKNATVNLVGHPRQDADDPQGPNGGRSGMTANEETSGKKELTISVRGEDGQR
                     RDFTFEMPHPQGKPPGELTLNEVFPPELLALLEEHSAARPVTEDETGQEQDPSAAIFE
                     AEARMAGSQITDTGNVQPPSPHVPETELGAVDD"
     gene            3083..5848
                     /locus_tag="VGR29_07155"
     CDS             3083..5848
                     /locus_tag="VGR29_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073405.1"
                     /translation="MSTTQPQRASRGRSRVLACFTVLMFLLSLMPAAASAMPALTTDQ
                     FSQLDAQTTLAQTGSFIEVHVSACETGATGTAQELRDLCHENGVAGVQMDITSVDPAL
                     GVNLVDKTTERINDAGPGITNTGTIPAGEYLIDVDLPGDGNRFVVGCEFFDREEIVPA
                     TPADAQEFRVTVPQGEDIVCDLFIIPQNGTGANAAVQPAQDLASLDFTVRYCDRTDLT
                     GDDRSFDALSANCTTVPAPPTAPPVPISLSIGMPGGEVSTRPLDAEGSVTFADLPNGN
                     YPVFSNVDRDNAGEYLFCTYEGQPRYEKTFDANGTTTFTNLLGEEIACDWFVVYVPQE
                     APPPPADQAASITANLVSCPRNYDVAANGEDAAAFATNCTAPAADVLMTLTGTDGTRT
                     EVTSTADGVAAFPNLEPDTYTLFSGIPLEAASEYVFCMGGDGVETREALSDRGVATLE
                     DIGTSQIECTWYIVPEDLRGEETGATVTVHLAACPVEYAGDQFYADCHANGVADQQYT
                     LSGPNGEVTATTTMPADPGPGVATFTELPAGEYTLAGGPPQDFGSVALYCSDPATNQP
                     IGATMEGGKARFSVAEQQSVLCDWYFIPVDARGEVTPTPTPAQAQRAEILVTLFGCKE
                     GTVAAGATFAQLDEACNAPINGVPFSLGIPGGTPLTANTGVSGEGAVRFYELRPGDYV
                     MTPSLPANYTSAAVYCQIGDGDVYQKSLQSGSTNFVDLDGEQMACSWFANPVKQVVQQ
                     PAGPTGSITVREFLCEGDRGSIKDWERECVPGATDTAFTLASSDGAVTRNATPNQQGV
                     LVFAELPDGYYELTQDDGVWCKAAAERVDSRSRVIVRDGGNTDVFIYECGQVTNLPDT
                     GSGTARIGGSSMQDASVLLAALAIPAFAAALWQLQRTKPEPVRVRAIRSVECTDPSDG
                     GNRIRFR"
     gene            6047..6970
                     /locus_tag="VGR29_07160"
     CDS             6047..6970
                     /locus_tag="VGR29_07160"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEV2073406.1"
                     /translation="MKYTRRQILGSVVGGLVAGGAGSAAIASSGVQLPTWGDAPAGSQ
                     KAGPLGLVSGAQQAQVPGEIPVSIVIPAADVDAEVERTKIVDGQMLDPSGPWIVAWYE
                     GTALAGEIGNAVMSGHVDYWDVGPAVFRNIANLGQGSEITLYGADGGTYTYGVEYIER
                     IEVATLTQEKLNQIVGPTDHPAVTLITCGGEFNYDAGEYYERDIIRARILGDTGQASG
                     TTDGAQEPADPAPAAAGLVEGGQATIVGGNVNVRADATTASEAVSVLSLGTTVTITGA
                     SREADGYVWWPITADDGISGWVVEDFLTPAG"
     gene            complement(7190..7414)
                     /locus_tag="VGR29_07165"
     CDS             complement(7190..7414)
                     /locus_tag="VGR29_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2073407.1"
                     /translation="MPNPDRIPEHISVLESLSHEHFTAEELAELLGVNVPVIVAAVRR
                     GELDAYTVDHRIVDIRRSDVLKWLNERRNS"
     gene            complement(7557..8882)
                     /gene="aspS"
                     /locus_tag="VGR29_07170"
     CDS             complement(7557..8882)
                     /gene="aspS"
                     /locus_tag="VGR29_07170"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEV2073408.1"
                     /translation="MSASSSFPATTPVLRTLAWELPQHIGERVLLQGWLHHQRHLKAV
                     DFLLLRDRSGIAQVVIEEPDLRAQLAALPHETVLDVQGYAAASPQAPNGVELHDPELT
                     VLSEGTGEPPIELFRPEMQALLATRLDHAAISLRHPVRAMTQRLLAAAMHGFRQSLMA
                     EGFTEISTPKLLEAAPEGGANVFSVDYFGRPAYLAQSPQLYKQMMVGALERVFEVGPV
                     FRAEPHDTSRHVSQFLSLDAEMGFIRDHQDLMQVVRDVLAGMMVAMAAVDGPVSVPIP
                     DVPKEIPQIHFREALTLLGKALGQDLRDEPDLAPEHERWLGEWADREHHSQWLFVTGY
                     PTRKRPFYTHPQPDDPRWSNSFDLLFRGLEIMTGGQRLSHHADYLAALGERRIDPEPF
                     RGYLEAFRHGMPPHGGFALGVERMIRQITGAQNVRDVMLFPRDMSRLAP"
     gene            complement(9369..10316)
                     /locus_tag="VGR29_07175"
     CDS             complement(9369..10316)
                     /locus_tag="VGR29_07175"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEV2073409.1"
                     /translation="MRDIAIFSASAHPELAQEICETLGKPLYEAEISRFSNDCLQAQL
                     HANCREQDVFLIQPLVPPVQEHLMELLLMADAARGASAARITVVIPHYAYARSDKKDA
                     PRISIGGRLVADLLVTAGADRVLTMALHAPQVHGFFSVPVDHLNALNVIAEHFQPRDL
                     SNTVVVSPDLGNAKSATQFARLLGLPVAAGSKRRLADDKVVIDAIVGDVDGKDIIVLD
                     DEIATGGSMLELIDRLGDRNVGHISIACTHGIFSGKAIERFSSTSRIDEIVTTNTVPI
                     PPEKRLPNMTVLSIAPLLAEAIHNIHVGESVSQLFVHTL"
     gene            complement(10508..>12392)
                     /locus_tag="VGR29_07180"
     CDS             complement(10508..>12392)
                     /locus_tag="VGR29_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEV2073410.1"
                     /translation="PVPSSISRAARKQGTRADGFTVADDIDTIPPVPIHAPARTRGHT
                     IVAWTLRAAIMLVLVSTIGYFGRERLAVSMDDIRDRIPVDVQQSLGIANTDEPDELAI
                     NTAPEGPDAENTGSDGTNGASTAGQPIAEPTLAPPDAPVETEVAQSETDEVVAEQEDL
                     TANIGGATTELPPTSDAANETDDPTDPLAPTETTIPPTEQPTPTEIPVEPTAVPTGIL
                     VQVAVVPTEIPTDVPTVAPTEEPTPEPTAVPTEVPTVEPTSIPTEAPTPEPTTAPTVA
                     PTIAPTEEPTPEPTAVPTEEPTEIPTEAPTAAPAEIPTEAPTATAAVDPTADLQPTIE
                     SQEPSVLPETTPEQAVVSNGFRYTIEGASVGPTIRELPEINSVGGYGEWVVLSVYGQN
                     LSDEAQVFDMANFRLIADGQEVQVDVGNAWVSGLAGNTPAYGNTDAIQWAPGEAHRFT
                     LTFLAPPAAESLVLQAGDQTIDLSPALDNPTSLLGDDQTPVASDLLQGTVVEVIDGET
                     IVVEIDGVQQTVRYLGVDAPTGDACYAEDAADANGSLVAGQTVTIERQATDTDARGNW
                     VRDVWVTNDAGQPVLVSQQLVLAGAAEADISDPNTRFEGWLQQSQEAAKDADAGIWGT
                     CGE"
BASE COUNT         2206 a         3771 c         3933 g         2482 t
ORIGIN      
        1 cgaccttctt ccgcctcatc gtcgcacttc caatcctgct cctgcttggt gccgtctcgg
       61 gtggggctgg agactggtcc tctgacaaca tggccagtgc ggccggagtg gccggtgtcc
      121 acttcttcgc tccgatcctg atgatcgtgt ttcgccagaa gtacccccga tggtggttcg
      181 actggaacct ggagctgcaa cggttctcga accgggtcac gacctacctg gccttgatgg
      241 acgatcgcta cccgtcgacc gacgaacagc aggccgtgca tctggactat gcctacccgg
      301 acgtaaggcg ggacctgaac cgctggctgc cgctggtgaa gtggttcctg gccatccccc
      361 atgtcatcgt cctggtggtg ctgaatattg tggccgtggt tgccgtcgtc atggcgtggg
      421 tcgccattct ggtgacgggc cgctatccac gcgggctgtt caactatgtg gaaggcgtct
      481 ttcgctggaa caaccgggtc attggctatg cggtgacact tgtcacggac acgtatccgc
      541 catttcgcct gtccgaataa gttgcctcgc ccggcctgaa cgcatcaaaa cgcatcaatg
      601 ggcgggtcag gagctgagaa tcatgacggg tagcggcaca gaccctgcgc ttcccaggac
      661 attgagaaga tgatgctcgc gccggatgca agcctgtcgt cgtggatagt tctttcgact
      721 ctccgttgcc gtcttcggcc ctggctggaa acgagccgtc ttaccccaat cggcagaagt
      781 gattgtcggg gtctcatcgt caggcacgtc agcggaggaa catcggacca tgaaacagat
      841 ccgtgcgctg tcgtggtcgc ttccggcatc aatcagggtc gtgggcgttg cgctcatcgc
      901 gaccatcgtc ctatgtgcca tggacgcgtc ggaaccggta gagtcccacg atactcccgc
      961 cagcaccctt cggatctctg tggtccatta cctggaggct ggcggttaca ccaacgccgg
     1021 acaggcagag ggccagatgt gcctgccgtt tggtacggtc ggcccgtggg agaccggtgg
     1081 gccacagctc attgtcacgg gcaacaccgg ccacatcgtc gggatcgtgg atgtcatgca
     1141 gggcacgttc gagcagattg atgagcgcat cggctgcgta gtacgggccg agttgcccat
     1201 cgtcgacgcg tcgttattca ccttcgccgt agccgatgtg tacaacgaga cggtgacccg
     1261 tgacgccttg gtagccgccg gttgggtcta cgaggttgag gtggatctgc cgtagattgg
     1321 ggcgtgccga tgggtgcctt tctagcttgg ttgctcgaat cgtctcccag gctacaactg
     1381 attccgaggg ccttcgctgc ctccggaacc agtcatgaaa cgattgattc gcatgtgttt
     1441 ccagcagaac aagcgccttc atgatgatcc acccgaacgt gtctcaactt tggggacccg
     1501 ggttttcctc agcgcctcgg cggtcgactt gataaagctg ctgagccagg caacgtctat
     1561 ccatcgatcg cggggaaccc ggagatagtt cctggcgttc ggatacgcgg ggatgcccgt
     1621 agggtcctgg acaaaggaca agccgggagc ggtcttcttg acgaacaact ggttgtcgca
     1681 gaggagcgca accaccaccc cttcgtgata tacggcgtac tcaccgaaca tcttcttcgt
     1741 cgtgacggga cagctgcctt gcagctgctg gaccagatcg tccgcagtct cttgattggt
     1801 tgccattgtt acagtcccgc aatcatcagc gaaatccttt ttcagaggca aacctggcga
     1861 gattgagcag gatatctccc tgcatcggac accatctcac tagagacttc cctcttcctg
     1921 gctctgttca gtcggctgcc cacagtatgt gcaggggttc ggcgattagt cgtcgactgc
     1981 acccagctcg gtttcgggta catgcggcga aggaggctga acattcccgg tatctgtaat
     2041 ctggcttcct gccattctcg cctcggcttc gaaaatggcg gcggacggat cctgctcctg
     2101 tcccgtctca tcttcggtaa cgggccttgc tgcggagtgc tcttccagca atgccagcag
     2161 ctcgggggga aagacctcat tcagcgtcaa ctcgccaggc ggtttccctt gcggatgtgg
     2221 catctcgaag gtgaagtcgc gacgttggcc gtcctcaccc ctcacagaga tcgtcagctc
     2281 tttcttgccg gaggtctcct cgttcgccgt catccctgat cttcctccat ttggtccctg
     2341 cgggtcgtct gcgtcctgcc tgggatgtcc gacaaggttc accgttgcat tcttgacctc
     2401 ttcaatgtca acaccggctg cttccagggt ttcgaggtcg agtggcttgt cctggtcacg
     2461 atgggccctg ccaacgtgct catgtccgtc gctatcggtc cagcgaatta ccgtttcctc
     2521 accgccctgc tcactgttcg tcgccatgga ctgttgctcc tgtcactccc cgtccctggt
     2581 tgcgtggatc gaccattgcc ccaaaaccgg gggcggtcac caggcatcgt tcctgccagc
     2641 cttgctggac ggacatctct tcccgaatgc attcctggtg ccctgattaa caccttccag
     2701 tatgcaggat tagcaccaca tataacccgg agactcagca cgagtccaac ggcgtttgcc
     2761 ccctcgatga tgtcgcgatt cggctcctgc acctccaacc ctgttgcgcc attacggatg
     2821 aactcaggcg cgatcatggc gacatgccga tcccaggcaa ccacaagggg tacggtgacg
     2881 gcatccgcaa tacaagggga aatgcaggtt ccggcgccca ggagagccgt tctcgccccc
     2941 aaggcgtatg atgaaggtgt acgtacctgt ctcatcacct gtccggaggg gatcgaaacc
     3001 tcgaccggag cccgtgcgtt attgagggga cgagcatcct ggttacgttt catcagatgc
     3061 catggacgag gagagcatga cgatgtctac gacacagcca caacgtgcat cccgcgggcg
     3121 gtcccgcgtg ttggcatgct tcacagtgct gatgttcctg ctgtcattga tgcccgcggc
     3181 cgcgagtgcc atgccggccc tgacaacgga ccagttcagt caactggatg cgcagacgac
     3241 gcttgcccag acgggcagct ttatagaggt gcatgtcagc gcctgtgaaa ccggggcgac
     3301 cggaacggcg caggagctgc gagacctctg tcacgaaaac ggcgttgccg gcgtccagat
     3361 ggatatcacc tccgttgatc cggcgttggg ggtcaacctg gtcgacaaga cgacggagcg
     3421 catcaacgat gccggacccg gtatcaccaa caccggcacc atcccggccg gtgagtatct
     3481 catcgatgtt gatctccccg gggatggcaa ccgctttgtt gtcggatgcg agtttttcga
     3541 tcgggaagag atcgttcctg ccacacctgc cgacgcccag gaattcaggg tcacggtgcc
     3601 gcagggcgag gacatcgtgt gcgatctctt catcattccc cagaatggaa ccggtgcgaa
     3661 cgcggccgtc cagcctgcgc aggatctcgc cagcctcgat ttcaccgtcc ggtattgcga
     3721 tcgcaccgat ctgaccggtg atgaccgctc gttcgatgcg ctttcggcca actgcacgac
     3781 ggttccggct ccgcccacgg caccaccggt gccaatctcc ctcagtatcg gcatgccagg
     3841 tggcgaagtc tccacgcggc cgctcgatgc agaggggagt gtcacctttg ccgatctgcc
     3901 gaatggcaac tacccggtct tcagcaacgt agacagggac aatgccgggg aatatctctt
     3961 ctgcacctac gaggggcagc cacgatacga gaagacgttc gacgctaacg gcacgacgac
     4021 gttcacgaat ctgttgggtg aggagattgc ctgcgactgg ttcgtggtct acgtcccgca
     4081 ggaagctccg ccacctccgg ctgaccaggc ggccagcatc acggcgaacc tggtctcatg
     4141 cccacggaac tacgatgtcg ccgcgaacgg cgaagacgcc gcggcgtttg ccacgaactg
     4201 caccgcgcca gccgccgatg tgctgatgac gctgaccggc acggatggca caagaactga
     4261 ggtcacgagc accgcggacg gtgtggcggc gttcccgaat cttgaacccg atacctacac
     4321 gctcttcagc ggcatcccat tggaagccgc cagcgagtac gtcttctgta tgggcggcga
     4381 tggcgtcgag acgcgggagg cgttgagtga ccgcggcgtg gccacgcttg aggacatcgg
     4441 cacaagccag atcgagtgca cctggtacat cgttccggag gacctgcggg gcgaggagac
     4501 cggcgcgacc gtcaccgtcc atctcgccgc ctgcccggtg gagtatgcgg gcgaccagtt
     4561 ctatgccgat tgccatgcga acggcgtagc agatcagcaa tacacgctct ccggcccgaa
     4621 cggtgaggta accgcgacca ccaccatgcc ggccgatcct ggcccaggtg tggcgacctt
     4681 caccgagctt ccggccggtg aatacaccct ggcgggaggt cctccccagg atttcgggtc
     4741 cgtggctctc tactgcagcg accccgccac caaccaaccc atcggcgcga cgatggaggg
     4801 tggcaaggcc cgcttctccg tggctgagca gcagagcgtg ctctgcgatt ggtactttat
     4861 ccctgtagac gcccgtggtg aggtcacacc cacccccaca ccggcgcagg cacagcgtgc
     4921 cgagatcctg gtgacgctct tcgggtgtaa ggaaggcacg gtcgccgctg gcgccacgtt
     4981 cgctcagctt gacgaggcat gcaacgcgcc gatcaatggc gttcccttct cgctcgggat
     5041 tccgggcggc acgccgctca ccgccaatac cggggtctcc ggtgaaggag ctgttcgctt
     5101 ctacgagctg cgacctggcg actacgtgat gacaccgtcg cttccggcga actacaccag
     5161 tgccgcggtt tactgtcaga tcggcgatgg tgatgtctac caaaagagcc tccagagtgg
     5221 ctcgacgaac ttcgtcgatc tggacggtga gcagatggcg tgctcctggt tcgccaaccc
     5281 ggtgaagcag gtcgtacagc agcccgctgg gcccaccggg tcgattaccg tccgggagtt
     5341 cctctgcgag ggggacaggg gctcgatcaa ggactgggag cgcgagtgcg ttccgggtgc
     5401 cacagatacg gcatttaccc tggcttccag cgatggcgcg gtgacacgta acgccacccc
     5461 gaaccagcag ggcgtgcttg tctttgcgga gttaccggat ggctactacg agttgaccca
     5521 ggacgatggt gtctggtgca aggccgctgc cgagcgggtg gatagccgga gccgggtcat
     5581 cgttcgcgat ggtggcaata ccgatgtctt tatctatgaa tgtggccagg tcacgaacct
     5641 gccggacacg ggttccggca ctgcccgaat agggggctcg tcgatgcagg acgcgtcggt
     5701 gctgctcgcc gcattggcga tcccggcctt tgccgcggcg ctttggcagc tgcaacgcac
     5761 gaaaccggaa ccggtacgcg tccgggccat tcgttcggtc gaatgcactg acccttcgga
     5821 tggcgggaac cggatccgct tccgctagtc caggccgagg cggtacgccg tacaccggga
     5881 atgcggcggc aggtggggca tccgcccctg atctgcgcag aatttgcggt accatgaact
     5941 cggtgcgggg cacgcacgat gtcgtatcac ccgaccccgc acagggctca atcaatgagc
     6001 ggaggcggtg gttcatgcca tcgcaatgac acatgaggaa cgacgtatga agtacacccg
     6061 caggcaaata ctggggtcag tggttggcgg acttgttgct ggcggtgccg ggagcgcggc
     6121 gatcgccagt tccggggtgc agctgccgac ctggggggat gcccccgccg gaagccagaa
     6181 ggccgggcca ttggggctcg tctccggtgc gcagcaggcg caggtcccgg gggagattcc
     6241 agtctcgatc gtgattcccg cggccgatgt ggacgccgag gtcgagcgga ccaagatcgt
     6301 cgatggccag atgctggatc caagcggccc gtggattgtg gcctggtatg aggggactgc
     6361 tctcgccggg gagatcggca acgcggtcat gtccgggcac gtggactact gggacgtcgg
     6421 cccggcggtc ttccggaata tcgccaacct cggccagggg tcggagatca ccctctacgg
     6481 tgccgatggc ggcacctaca cctatggtgt tgagtacatc gagcgcatcg aggtcgcgac
     6541 cctgacccag gagaagctca accagatcgt cggcccgacc gaccatccgg ccgtgaccct
     6601 gatcacctgt ggtggagagt ttaactacga tgcgggcgag tattacgagc gggatatcat
     6661 ccgcgcccgc atcctaggcg ataccggcca agcctccggc accacggacg gagcccagga
     6721 gcccgcggac ccagccccgg ctgccgcagg gctggtcgaa ggtggccagg caaccattgt
     6781 tggtggcaac gtcaatgtcc gcgccgatgc cacgacggca tccgaggcgg tgagcgtcct
     6841 cagcctggga accacagtga cgattaccgg tgcgtcccgc gaagcggatg gatacgtatg
     6901 gtggccaatc acggccgatg acggcatatc tggatgggtc gtcgaggatt tcctgacacc
     6961 tgccgggtga cggcctgaga cgacatctgt aacagaaggc cggtcctgat tttgagaccg
     7021 gccttctgct ttgctgcatc ccttggagct attttcagag aggttgcatg tctgtaaagc
     7081 ctgtcatgtg taggggagga ccttgtgtcc tcccgccccc ggacaaggga agacacaagg
     7141 tcctccccta cgtaggcggc tggtcaatgg tcatgcaaat cgctccaggt caggaattcc
     7201 tgcgctcgtt cagccacttg aggacgtcag agcgcctgat atccacaatc cggtggtcca
     7261 ccgtgtaggc gtcgagctcc cctcggcgaa cggccgccac gatgaccgga acattcacgc
     7321 caaggagttc cgccagttct tcagccgtaa agtgctcgtg cgagagtgat tcaagcacac
     7381 tgatatgttc cgggatacgg tcggggttcg gcatcgtcga cctcctgcaa tgaacgacgt
     7441 tggcgtcgca gcgtggtgac gtgttgcggt ctgtccttcc attggcgcct tcgtgctgtg
     7501 tatggtgcca ggacctcaac ataataccgc agacgatgtg ccctgctcca gtgacactac
     7561 ggtgccaggc gggacatatc tcgcggaaag agcatgacgt cgcggacgtt ctgcgcgcca
     7621 gtgatctgcc ggatcatccg ttccacgccg agtgcgaatc cgccgtgcgg aggcatgcca
     7681 tgccggaacg cctcgaggta cccgcggaaa ggctccgggt caatccgccg ctcgcccaat
     7741 gcggcgagat aatccgcatg gtgagagagt cgctgtcccc cggtcatgat ctccagcccg
     7801 cgaaagagca ggtcgaagct gttggaccac ctcggatcat ccggctgtgg atgggtgtag
     7861 aagggccgct tgcgcgtcgg gtagccggtg acgaagagcc attgggaatg atgttcgcga
     7921 tctgcccact cgccgagcca tcgctcatgt tccggggcaa gatccggttc gtcccgcagg
     7981 tcctgtccga gcgccttgcc gaggagcgtc agggcttccc ggaagtggat ctgcggaatc
     8041 tcctttggga catcaggaat cgggacggag acaggaccgt caacggcggc cattgccacc
     8101 atcatccctg caaggacatc ccggacaacc tgcatgaggt cctgatgatc ccggataaat
     8161 cccatctcgg cgtcgaggga gagaaactgg ctgacgtggc gtgatgtgtc gtgcggctct
     8221 gcacggaata ccggtccaac ctcgaagaca cgctcaagtg cgcccaccat catctgcttg
     8281 taaagctgtg ggctttgggc aaggtacgct gggcgcccga aatagtcgac gctgaagaca
     8341 ttcgccccac cctctggtgc ggcttccagc aacttgggag tcgagatctc ggtgaagccc
     8401 tctgccatca gggactgccg gaagccatgc atcgccgccg cgagcaggcg ttgggtcatg
     8461 gcgcggaccg gatgtcggag cgagatcgcc gcgtgatcca ggcgggtcgc aaggagtgcc
     8521 tgcatctcgg gacggaagag ttctattggc ggctctccgg tcccctcact caggacggtg
     8581 agttccgggt catgcagctc caccccgttc ggtgcctggg gcgatgcagc ggcatacccc
     8641 tgcacgtcga gcaccgtctc atggggcagg gccgcaagtt gcgcccggag gtcgggctcc
     8701 tcgatgacga cctgcgcaat gccggaccgg tcccgcagga gaagaaaatc gacggccttg
     8761 aggtgccgtt ggtgatgcag ccatccctgc aggagtacgc gctccccgat gtgctgcgga
     8821 agttcccacg cgagggtgcg caggaccggc gtggttgccg ggaacgaaga actggcggac
     8881 atggtgccac ctccgaaaaa atgccagacg ccccctccgc catgcggacg agggggcgtc
     8941 tcgtggtgcc accgcaattc gccggatctc cggctatccc gggatcgcga cctttctgga
     9001 gcccggtaac gggggcgatc cggcatgacc tactggcaac aatgccgtgt tcagacatgc
     9061 gctcgagagg gtcttcgctc caggatgatc gccacgcttt caccaaccgt ggctcgcttg
     9121 tggatcagcg cccgcaggta ctcggctccg tcaacgcgtt gtctacaggt tacgggaacg
     9181 acggggcttt gtcatctgcc tgcgacagat gccggtaccg gaccaatagc cgagtaggga
     9241 cggtacttgc gaagcaatgt ccgcccgatg tccgtagggg cccaataggc ctggtggata
     9301 ttggcgcagg gggagccgcg tgtccgccga cgcgatcagt atccgcgact gacaggccga
     9361 tgtgctttct acaaggtatg cacgaacaac tggctgacgg attcacctac atggatattg
     9421 tggatcgcct ccgccagaag gggggcgatg gagagcacag tcatgttcgg cagtcgcttc
     9481 tccggtggga tcggcacggt attggtcgtc acgatctcgt cgatccggct cgtgctggaa
     9541 aatcgctcga ttgccttgcc ggagaagatg ccgtgggtac aggcgatcga gatgtgtccc
     9601 acgttccggt cgcccaggcg atcgatgagc tctagcatgg agccgcctgt cgcgatctcg
     9661 tcgtcgagga cgatgatgtc cttgccatca acatctccca caatcgcgtc gatgaccacc
     9721 ttgtcgtctg ccagccgccg cttgctgccg gccgccacgg gcaggcccag cagacgggca
     9781 aactgtgtgg cggatttggc attgccgagg tctggcgaga cgacgactgt gttggagaga
     9841 tcgcgtggct ggaaatgctc ggcgattacg ttgagcgcgt tcaggtggtc caccgggacg
     9901 ctgaagaaac catgcacctg gggcgcatgg agcgccatgg tcaggacacg gtcggcgccg
     9961 gcggtcacaa gcaggtccgc cacgaggcga cctccaatgg agatgcgtgg cgcatccttt
    10021 ttgtcggagc gggcatatgc gtagtggggg atgacgacgg taatgcgggc tgcggaagcg
    10081 ccgcgggcag catccgccat caggagcagc tccatcaggt gttcctggac gggtggtacc
    10141 aggggttgga tcagaaagac atcctgctcc cggcagttgg cgtggagttg cgcctgcagg
    10201 cagtcgttgc tgaagcggct gatctctgcc tcgtagagcg gtttgccgag tgtttcgcag
    10261 atttcctggg cgagctcggg atgagcagaa gcggaaaaga tagcgatgtc acgcattggc
    10321 cggtgcgtcc ttctgaaaag gttgaaaggg ggacggccgc atgggggacc gcgcctctgc
    10381 tgttctctcg tgatcctacc ctgtgtatag agacgcgcca aggtagctgt cctgaagaag
    10441 ggaagatccc cgatcgaata tatgccgatc gggaatcttc ttcatgacgc tctgtgctta
    10501 gatagagtta ctctccgcag gtcccccaga taccggcgtc ggcatccttg gcggcctcct
    10561 gcgactgctg gagccagcct tcgaatcgag tgttcgggtc actgatgtcc gcttccgcag
    10621 caccggcgag gacgagctgc tgggagacga gcaccggttg gccggcatcg ttggtgaccc
    10681 agacatcgcg gacccagttc ccacgggcgt cggtgtcggt cgcctgtcgc tcgatggtca
    10741 ccgtctgccc tgcgacgagg gatccgtttg catcggctgc gtcctctgcg tagcaggcgt
    10801 ctcctgtcgg cgcgtcaacc ccgagatagc ggaccgtttg ctggacaccg tcgatctcga
    10861 ccacgatggt ctcgccatcg atcacctcga ccacggtccc ttgcaggaga tccgacgcca
    10921 ccggcgtctg gtcgtcgcca agcagtgacg tggggttgtc aagcgccggg ctcaggtcga
    10981 tcgtctggtc tcccgcctgg agcaccagcg attccgcggc tggcggtgcc aggaaggtca
    11041 gggtgaaacg atgggcctcg cccggagccc actggatcgc atcagtgttg ccataggcag
    11101 gggtgttacc cgccaggccg ctgacccagg cgttgccaac atccacctgt acttcctggc
    11161 catccgcgat gagccggaag ttggccatgt caaagacctg cgcctcgtcg gacagattct
    11221 ggccgtagac gctcaggacc acccattcgc catagccgcc gacggagttg atctccggga
    11281 gttcccggat cgtcggacct accgacgccc cctcgatcgt gtagcggaag ccattcgaga
    11341 ctactgcctg ctccggcgtc gtctccggca ggacgctcgg ctcctgggac tcgatcgttg
    11401 gctgcaggtc ggccgtcgga tcaacagcgg cagttgctgt gggcgcctcg gttgggatct
    11461 ctgccggagc tgctgtcggc gcttcggttg ggatctctgt cggttcctcg gtcggaaccg
    11521 ccgttggctc aggtgtaggt tcctctgttg gagcgatcgt cggtgcaacc gtcggtgcgg
    11581 ttgtcggctc aggtgtcggc gcttcggtcg gaatcgacgt aggctcgacc gttggcacct
    11641 cggtcggaac cgccgttggc tcgggtgtag gctcctctgt tggagcgact gtcggtacgt
    11701 cagtcggtat ctctgttggc acaactgcca cctggaccag aatccctgtc ggtaccgccg
    11761 ttggctctac cgggatctcg gtcggggtcg gctgctcagt cggcgggatg gtggtctcgg
    11821 tgggcgcaag cggatcggtc ggatcatctg tttcattcgc ggcatcgctg gtcggaggca
    11881 gctctgtggt cgccccgcca atattcgcgg tcaggtcttc ctgctcggcc acgacctcgt
    11941 ccgtctcgct ttgggcgacc tcggtctcca ctggagcatc aggcggcgcc agtgtcggct
    12001 ctgctatcgg ctgacctgcg gtagaggcgc cgttggtccc gtcgctaccc gtgttctccg
    12061 catccggtcc ctcaggtgcg gtgttgatgg caagctcatc cggctcatct gtattcgcga
    12121 taccgagcga ttgctggaca tccaccggga tgcgatccct gatatcgtcc atgctgaccg
    12181 ccaggcgctc gcgtccgaag tagccgattg tgctgacgag aaccagcatg atcgccgccc
    12241 ggagcgtcca cgcgacgatc gtatgtcccc gtgtgcgagc cggtgcatgt atggggacag
    12301 gtgggatggt gtcgatgtca tcggcgacgg tgaagccgtc ggcccgcgtg ccttgcttcc
    12361 gggcagcgcg gctgatcgat gagggcacgg gc
//
LOCUS       DASYWM010000175         6299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_288284, whole genome shotgun sequence.
ACCESSION   DASYWM010000175 DASYWM010000000
VERSION     DASYWM010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288284"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..854
                     /locus_tag="VGR29_07185"
     CDS             186..854
                     /locus_tag="VGR29_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073411.1"
                     /translation="MNHVRRWTRYFILFGLIFASLVVAGPAAAQDGEEAAEGFPLTAF
                     AAYCEPEHAGPFVGCTPWEGVTVTFVAIDTNFSTSCVTEAGERAATCSVEVPFGSTIE
                     ASIDPATVPAGYVLQGQSLQQLTIPDGPPEGEFGGPSFILLESSDIEGEDPADEGDEY
                     VPPVIGDEPRTDHEPATVTSLPSTGTGPEVDDAVLGAVLAIMLAGLGALVSAGAIGLH
                     RAVR"
     gene            complement(965..2623)
                     /locus_tag="VGR29_07190"
     CDS             complement(965..2623)
                     /locus_tag="VGR29_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073412.1"
                     /translation="MRSFALPTMGPDNPRARWYVLAICCVALFMAILDNLIVNVALPT
                     ISDDLSPTTAQLQWILSAYVLVFASLQITAGGLGDRFGRKRFFIFGLILFTATSGLAA
                     LVPSTGWLITARAAQGIGAAFIMPLSLSIISAAFPPAERGKAIGIWSAISMSGIAIGP
                     IIGGIIVDAWSWHWIFLINVPIGIVSIIGAQVILQETRDTTGHAPTDIPGTITVTAAI
                     AALTWGLIEAGERGWSDPAIVASLTAAVVLVGLFVVIENRSDNPMVPMRFFKSRNFTG
                     SNLVAVATSFLISGLAFAMTMYYQNVHGFSAIRTGFTMLPSVIPMVIMGAASGALLAR
                     LGARNMIATGMVIGAAGIFLLGRVGIDTPFLALAPALVIFGTGMGLVFAPMMTTLMNS
                     VETEKSGVASAVNGAIRETGFAFGIALLGTIMNQRYRSEIRSSEEFNGLRDASDPQLG
                     PLQPVLDLIERGINFGGRVIQNEKLFPGLPESVTEPVRIASSRAFVAGMEQAFVISSV
                     ATVAAAVVAWFLIRDEVPVAQEREQVEGRSGNRVSATSSPVSAD"
     gene            2916..3527
                     /locus_tag="VGR29_07195"
     CDS             2916..3527
                     /locus_tag="VGR29_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2073413.1"
                     /translation="MGNREELLAGARKCLLEKGYVQTTARDIASASGVSLAAIGYHFG
                     TKEALLQEAMVEANIEWGERIKNAVSVMQAPGDTAFPEWFEQCWTRIITASREDQRLL
                     QASIEVLLNVDQETPVCSSLTNGMQCARDALAVMFGHGEDDLDPHEAAAVGAFYHALM
                     IGVRLLHAATPERAPGPDDITLAMQTISRKFCEKSTEADPADR"
     gene            complement(3809..4201)
                     /locus_tag="VGR29_07200"
     CDS             complement(3809..4201)
                     /locus_tag="VGR29_07200"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEV2073414.1"
                     /translation="MEDRNTLIARFQITNREETQLPGVDGDWANDARMAKTFTEGIEG
                     GSEGLFITSGQEEGKCAYMATERIIGTLSDGRSGSFVVQHGGLEANSNSWFGYIVNGT
                     GSGDLAGVAGSAVISHDDKGAHFTFVLE"
     gene            complement(4229..4687)
                     /locus_tag="VGR29_07205"
     CDS             complement(4229..4687)
                     /locus_tag="VGR29_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2073415.1"
                     /translation="MAVQDIGHPAYGCFDLDASLQFYALFGIEESFRLVKEDGSVRLV
                     YLHVAGDRFIELFPGGPPPEERASQAQQSYRHLCLMIDDIEGMVEHLRSNGITIDTEP
                     KVGLDFNTQAWVKDPDGNPIELMQISPQSPQRAIADGTAFPESDILVTPS"
     gene            4809..5594
                     /locus_tag="VGR29_07210"
     CDS             4809..5594
                     /locus_tag="VGR29_07210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2073416.1"
                     /translation="MTLSFDALAEQFDDQRGLPANALRHLVAFVDEIARGRTLTIVEP
                     GIGTGRISLPLAASGHRIVGIDISRPMLDACARKAATLRVEERVTLIEGDATAIPRED
                     DSCDVGIFASLLYLVRDWEGVLDEFARIVRPGGAVVWIRERTERGGALALWDIGWRTR
                     VETAGFRHQAASPAEDEILAALQRRWPDVITEPLASWTFGQSVGQARDGYGERLRSLY
                     PAIPDDTWSVLVQDFLHWAETLFLDPDGRLDGQVMLEAVVAWT"
     gene            complement(5698..6072)
                     /locus_tag="VGR29_07215"
     CDS             complement(5698..6072)
                     /locus_tag="VGR29_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2073417.1"
                     /translation="MLSTGSPIQNRIGNVFVPVSDMPRAVAWYSRLFGLEEGQTSHEG
                     GIYDVPMAGETRLILDANKPITEHSAQPLCLFLTNDIAASIDWLRELGVEIAGEPQDI
                     GSVIFATFNDPDGNPLMVCQRK"
BASE COUNT         1224 a         1864 c         1975 g         1236 t
ORIGIN      
        1 taatgaatcc ggtgggacgc caaccacatc aataaacatg ctgttgcagc tacctgtttg
       61 cgggaggaga gggacaggaa atgcattggt aatgcccgcg atgtcgggca atgcacaacg
      121 gacaggcacc cgggaggtca gacgcccagc gccgtggata cgattcagat cagggatgta
      181 ttgctatgaa tcatgtgcgt cgatggactc ggtatttcat attgttcgga ttaatattcg
      241 ccagtctcgt ggtggctggt ccggccgctg cccaggatgg ggaagaggcg gcggagggct
      301 tcccgctcac agccttcgct gcctattgcg agccggagca cgctgggcca ttcgtcggct
      361 gcacaccgtg ggaaggcgtg acggtcacct tcgtggcgat cgatacgaac ttctcgactt
      421 cgtgtgtcac tgaagccggg gagcgggctg ccacttgctc ggttgaagtg ccgttcggat
      481 ccaccatcga agcgtcgatc gatcccgcga cggttcccgc cgggtatgtc ttacagggcc
      541 agtcccttca gcaactgacg attcccgatg ggccccctga aggcgaattc ggcggcccaa
      601 gcttcatctt gctggagtcg agcgacatcg agggggaaga tcccgctgat gaaggcgacg
      661 agtacgtgcc cccggtcatc ggcgatgagc cccggacgga tcatgagccg gctaccgtga
      721 cctcgctccc aagcacaggc acgggtcctg aagtggatga tgcagttctc ggtgccgtgc
      781 tggccatcat gctggccggt ttgggagccc tcgtgtccgc tggcgccatt ggtctccacc
      841 gggccgttcg ctaggcgcca ctgaaggtca tcgttccata caaaggaagg ggagccatca
      901 tggctcccct tcctttgtat gacctacctg tcatcgcacc gtggatcgct gcaacggttc
      961 ttcgctagtc agcggacacg ggcgatgatg tggccgaaac ccggttgccg gaccttccct
     1021 ccacctgctc ccgttcctga gccacgggca cctcatcacg gatcaggaac caggcgacga
     1081 ccgccgcggc aaccgttgcc accgaggaga tcacgaaggc ttgctccatg ccggcgacga
     1141 aggcacgcga gctggcaatc cggaccggtt cggtgacgga ttccggcagt ccggggaaca
     1201 gcttttcatt ctggataacc cggccgccga agttgattcc ccgctcgatc aggtcaagga
     1261 ccggctgcaa cggcccaagc tgcggatcgc tcgcgtcccg caacccgttg aactcctcag
     1321 agctgcggat ctcgctgcgg taccgctggt tcatgatggt gccaagcagg gcgatgccga
     1381 aagcaaagcc tgtttcgcga atcgcgccat tgaccgccga ggcgacaccc gatttctcgg
     1441 tctccacgct gttcatgagt gtcgtcatca tcggagcgaa gacgagaccc atgccggtgc
     1501 caaagataac cagggcaggc gcgagggcga ggaatggcgt gtcgatccct acccgtccca
     1561 acaggaagat tccggctgca ccgatgacca tacccgtcgc gatcatgttg cgagccccga
     1621 gcctggcgag cagcgcgcca gaggccgcac ccatgatcac catcgggatc accgacggca
     1681 gcatcgtgaa gccggtgcgg atcgcgctga acccgtgcac gttctggtaa tacatcgtca
     1741 tcgcgaaggc gagcccggag atcaggaacg aggtcgcgac cgcgaccagg ttggagccgg
     1801 tgaagttgcg ggacttgaag aagcgcatcg gcaccattgg gttatcggat cggttctcga
     1861 tgaccacgaa gagcccgaca agtaccacgg ctgccgtgag actggcgacg atggcggggt
     1921 cgctccatcc tcgttcgcct gcctcgatca ggccccaggt caacgcggca atcgccgcag
     1981 taacggtgat cgtccccgga atatcggtgg gtgcgtggcc ggtagtgtcc cgcgtttcct
     2041 ggagaatcac ctgtgccccg atgatcgaca cgataccgat cggcacgttg atcaggaaga
     2101 tccagtgcca cgaccatgca tcgacgatga tgccaccgat gatggggcca atcgcgatgc
     2161 cggacatcga gatggcgctc cagatgccga tcgccttgcc gcgttccgcg ggcgggaatg
     2221 ccgcggagat gatggagagc gagagcggca tgatgaacgc cgcgccgatc ccctgtgccg
     2281 cccgggcagt aatcagccat ccggtcgatg gaacgagcgc cgccaagccg gaggttgctg
     2341 taaagagaat aaggccgaag atgaagaagc gcttacgtcc gaagcggtca ccaagcccgc
     2401 ccgccgtaat ctgcaacgat gcgaagacca gcacgtaagc ggaaaggatc cactgcaact
     2461 gcgcggtcgt tggcgagaga tcgtcggaga tcgtcggcag ggccacattg acgatcaggt
     2521 tatcgagaat ggccataaag agggcgacgc agcagatggc aagcacatac cacctggcgc
     2581 gtggattgtc tggacccatt gtaggaagtg caaaagaacg catggaaata ctcggctcct
     2641 tgtcaacgcg gcccgagcgg acttcgcgca tcggggagca cgattccggc agggcattac
     2701 atgtttgcgg cgagagcagt gtttagcctg ccgacgcctg agacccactc tgtctgcgta
     2761 tgcggacact tcgcgtccgc aaaaccggga gatgtctggt cggaatgtat ccgcctcttc
     2821 ccaaggcaaa cacgactcta ccggaatgtt gcacatttgt ctagtacaat tgtgcaaatg
     2881 agtttggaag tattctgcgg caatgggaag tgatcgtggg gaaccgggaa gaattactcg
     2941 caggagcgag aaagtgcctg ctcgaaaagg gctatgtgca gacgaccgcg cgggatatcg
     3001 cgagtgcgtc aggtgtgagc cttgccgcga taggctatca cttcggcacc aaggaagcgt
     3061 tgctgcagga agcgatggtc gaggcgaaca tcgagtgggg tgaacgaatc aagaatgcgg
     3121 tttcggtgat gcaggccccg ggcgatacgg cattcccgga atggttcgag caatgctgga
     3181 cccggatcat taccgcttct cgcgaggacc agcggttgct ccaggcatcg atcgaggtgc
     3241 tgctcaacgt cgatcaggaa acccccgtct gttccagcct gactaacggt atgcagtgcg
     3301 cacgggatgc gttggccgtg atgttcgggc acggggagga cgaccttgat cctcacgagg
     3361 ccgcggcggt tggcgccttc tatcatgcct tgatgatcgg cgttcggttg ctgcatgccg
     3421 cgacaccgga acgcgctccg ggcccggacg acatcacgct tgcaatgcag acgatctcgc
     3481 gcaagttctg cgagaaatcg acagaagccg atccagccga ccggtgatca ctggtgcgcc
     3541 gatcttgtca atcgattgaa cggggacgat tcactgtcgt ccccgttttc ttcgtgtctg
     3601 accggctggt ccttttcact attaggccga tcgccggcaa ctccgttccg tcagggacag
     3661 ctcgttgcgg caatggacaa tctccatgat gaaagaatgg ctcgatggga taatccacca
     3721 ggatcgtgat cgcgcaggaa tttcctccct catcgaaggc cgggctctct atcgaacggc
     3781 ccgcgatgct cccggagaaa tggggccgct actccagcac aaaggtgaag tgggcgcctt
     3841 tgtcgtcatg cgaaatgacg gcgctccccg ccacccccgc caggtcgcca ctccctgtgc
     3901 cgttcacgat gtagccaaac caggagtttg agttcgcttc gaggccgccg tgctgcacga
     3961 cgaacgagcc ggaccgtccg tccgagagtg tgccgataat gcgctccgtc gccatgtagg
     4021 cgcacttccc ttcttcctgg ccagaggtga tgaagagccc ctcactgcct ccctcgatcc
     4081 cttccgtgaa ggtttttgcc atcctggcgt cgttcgccca gtcaccatcg acgccgggta
     4141 gttgagtctc ttcccggttc gttatctgga accttgcgat cagagtgttc ctgtcttcca
     4201 tgatatggct cccccgtggt cgggctggct acgatggcgt caccaggatg tcgctctccg
     4261 ggaaggcagt tccgtcggcg atagcacgct ggggcgattg tggcgagatc tgcatcagct
     4321 cgatcgggtt gccgtccggg tccttcaccc atgcctgggt gttgaagtcc agcccaacct
     4381 tcggctcggt gtcgatggtg atgccattgc ttcgcaggtg ttccaccatg ccttcaatgt
     4441 cgtcgatcat caggcagagg tgacggtagc tctgctgcgc ctgtgatgca cgctcctcgg
     4501 gaggtggtcc tcccggaaag agctcgatga agcggtcacc ggcgacatgg aggtagacga
     4561 gccggacaga cccatcctcc ttcaccagcc ggaaggattc ctcgatcccg aacagtgcgt
     4621 agaactgaag cgacgcgtcg aggtcgaaac aaccgtaggc cgggtgaccg atatcctgaa
     4681 cagccatggt gaagataccc cctgtggcgg atactgcagc aacgagcaag cgaacgcgtc
     4741 gctattgtgg ggccgttgtt ctgatgtgtc acccttccgg tcggcaggcg accgggtgag
     4801 gagcagccat gacgctttcc ttcgatgccc ttgccgagca attcgatgat cagcgcgggc
     4861 taccggcgaa tgcacttcgg catctcgtgg cctttgtcga cgagattgcc aggggccgga
     4921 cgctcaccat tgtcgagcca ggcattggaa ccggtcgcat ctccctgcct ttggccgcat
     4981 ccgggcatcg gatcgttggc atcgatatct cccgcccgat gctcgatgct tgcgcccgga
     5041 aggccgccac actgcgtgtc gaggaacggg tcacgctgat cgagggcgat gcgaccgcga
     5101 tccctcgcga ggacgactcc tgcgatgtcg gcatctttgc ctcgctgctc tacctcgtca
     5161 gggactggga gggcgttctg gatgaattcg cgcggatcgt tcgtccaggg ggagccgtcg
     5221 tctggatacg ggagcggacg gagcggggtg gcgcactggc gctttgggac atcggctggc
     5281 ggacccgtgt cgagacggcc ggcttccgtc accaggctgc ctcgcctgcc gaggatgaga
     5341 tcctggcggc acttcagcgc cgctggcctg atgtgataac ggaaccactg gcatcctgga
     5401 catttggcca gagcgtggga caggcacggg acggctacgg tgagcggctt cgctcgctct
     5461 atcccgcgat cccggatgac acctggtccg tgctggtgca ggattttctg cactgggcgg
     5521 aaacattgtt tcttgatccg gatggacggc tcgatggcca ggtcatgctg gaggcagtcg
     5581 tcgcctggac gtgacccctg gcccggcctg tggccgagga cctgcgggcg cgacgcgagg
     5641 ctgcacagca ctcacctgta cggatgttcg gccgtgtgcc aggtcctgtt gctatcacta
     5701 tttgcgctgg cacaccatga gcgggttccc gtccggatcg ttgaacgtcg cgaagatgac
     5761 gctgccgata tcctgtggtt cgcctgcgat ctccacaccg agttcccgga gccagtcgat
     5821 tgacgcggca atgtcgttgg tcaggaagag gcagagtggc tgggcggaat gttccgtgat
     5881 tggcttgttt gcatccagaa tgagccgtgt ttcacctgcc atcgggacat cgtagatccc
     5941 accctcgtgc gaggtctggc cctcttcgag cccgaataac cggctgtacc aggcaacggc
     6001 acgcggcata tcgctcaccg ggacaaagac attgccgatc cggttctgga tggggctgcc
     6061 tgtcgaaagc atgatctctc cttcaaactg acatacgcaa aacgagacgc aagtctacag
     6121 ccgatcggcc gagggctcag ttgtttctgg gttttcatcc gcaggccagc agccgcaggt
     6181 tgcacaatga cgcttgccga tagggacacc accactacag gatcggcatc cgtattcccc
     6241 agtcatgggt tccgggacgg tggctccgag ggcaaggaat cggaggtaga gcccacgat
//
LOCUS       DASYWM010000176        31097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_420300, whole genome shotgun sequence.
ACCESSION   DASYWM010000176 DASYWM010000000
VERSION     DASYWM010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 31097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31097
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420300"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="VGR29_07220"
     CDS             complement(<1..1401)
                     /locus_tag="VGR29_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV2073418.1"
                     /translation="MAVDRDVRTTAVVPDIGPRQVLSNVGVNRRSYSSIPTVLEMPNL
                     VQIQVESFNWFVEEGLRELLDEISPITDHHRKMELYISEPRFDPPWSRMPKGEEQERA
                     KTDPRVAEVFCRERDITYAAPLRVSAKLVMKETGEIKETGPDGIFLGDFPVMTSDGTF
                     IINGAERVVVSQLVRSPGVYFERTQDPATGKYLSTAKLIPNRGAWLEFETSNRDVISV
                     KVDRKRKMPVTILLRAIGIENDEDLLAAFEDVDTKEDHRYIATTMEKENTKTRDDALV
                     ELYRRLRPGDPPTKDNASAMLENLFFNERRYDLARVGRYKLNGRLHRDNEAARTDERV
                     LRNEDLVAIIREMIRLNNGLSHPDDIDHLGNRRIRSVGELIQMHVRTGFQRLERGIRE
                     RMTLQDPETVTPQMLGSTTRPVMAAVREFFGGSQLSQFMDQLNPLAELTHKRRLSALG
                     PGGLSRDRAGMDVRDVH"
     gene            1955..3301
                     /locus_tag="VGR29_07225"
     CDS             1955..3301
                     /locus_tag="VGR29_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEV2073419.1"
                     /translation="MNSRTLRMWLRPGMMIKRWIALFMLATIVTSLGLAMALAWIYEN
                     YDFPYTIQPYVEWLTLQPIPHPWREVIIIVTGLAFLGFTIWRLSRSLIAPLLAGDTSG
                     RAFSEIVNDHRFGPAKAEFNVVAIGGGTGLSALLRGLKLQNVNLTAIVTVADDGGSTG
                     RIRNVFNMPAPGDIRNCLVALADNESLMGRLFHYRFDKEGSELSGHAFGNLFITALTQ
                     VTGSFEQGVIESAKVLNVRGRVLPSTLENITLCADLVDGSRVRGESAIAHDNPAISRI
                     YLDPDAPDAYRPALAAILNADLIVLGPGSLYTSVLPNLLVGGIREAILWSRGTTAYVC
                     NVATQHGETDDFGYEDHIAEVVEYLGDRKLDFAIVNSNAASDSAIRPEWQVSAVHYDG
                     KEFVADGVKIVAGDVVNDRNPLRHDPVKLAEILIGLARDRKDETAPLEVATTSSTT"
     gene            3421..4476
                     /gene="gap"
                     /locus_tag="VGR29_07230"
     CDS             3421..4476
                     /gene="gap"
                     /locus_tag="VGR29_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV2073420.1"
                     /translation="MVYKIGINGFGRIGRQVFKAVTEGGFGDLFEIVAVNDLTSNDTL
                     AHLLKYDSNYGSFDADISSSEEGITVDGKLLRVFEEKEPGNLPWSDLGIHLVIESTGR
                     FTKPEAARAHIDEAGAKKVVISAPLTTTADSSAGVDDAITVVLGVNEDRYDPKTHNII
                     SNASCTTNCLAPVAKVISDTFGIEHGMMTTVHSYTADQVLQDGPHKDLRRARGAAQNI
                     VPTSTGAARALSLVLPELEGKLDGFALRVPTPTVSIVDFVANTTRPATVDSVNEAFRS
                     ASQSDEMVGILGYSEEPLVSTDYKGDSRSSIVDALSTMVQGDNMVKVVSWYDNEWGYS
                     CRVADLAALICEQGFDS"
     gene            4648..5838
                     /locus_tag="VGR29_07235"
     CDS             4648..5838
                     /locus_tag="VGR29_07235"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV2073421.1"
                     /translation="MTKRSVASADVRGKRVLCRVDLNVPLEGGAIGDDTRIRSALPTI
                     EWLRDRGARVVLCSHLGRPKGKVVEDLRLAPVAEHLSRLLGQEVIAIPTITGTDAFQA
                     IGSIQDGDIVLLENLRFDPREEKNDPEFAAELAALADLYINDAFGAAHRAHASTEGIA
                     HLLPAYLGLLMQEEINTLGKLLTDPERPFTAIIGGAKVSDKIAVLQNLVTRVDALLIG
                     GGMANTFLLSQGKEVGASLVEEDRVGTAKDILETARQRGVDVLLPDDVVVARDIDAPE
                     GTVKPVGQISADEAIFDIGPDTAQRYADTISTQKTIFWNGPLGVTENPAFSMGTRVVA
                     EAVANSVGFTVVGGGDSAGAIEDLGYTSRIDHISTGGGASLEFLESRTLPGIAAIPDE
                     GEAE"
     gene            5835..6596
                     /gene="tpiA"
                     /locus_tag="VGR29_07240"
     CDS             5835..6596
                     /gene="tpiA"
                     /locus_tag="VGR29_07240"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV2073422.1"
                     /translation="MSTPLIVGNWKMNTSLDEAITLAKAAAAVADGAGAGTDVGICPP
                     FTWIVPVRDAVRGSSLRVGAQDCSAHDNGAYTGDISAKMLASCCTFTLVGHSERRSVH
                     GETDDLLQQKLHEALEAGLDVILCVGESAEERNTGIADRVVLRQIDATLDRTRLTDTS
                     RLTIAYEPVWAIGTGVAATEADASQMATLIREDLDARFGSSADHIRVLYGGSANDRNA
                     PAFLAAKNVDGLLVGSASLSADVFASLIEGARITP"
     gene            6720..6944
                     /gene="secG"
                     /locus_tag="VGR29_07245"
     CDS             6720..6944
                     /gene="secG"
                     /locus_tag="VGR29_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV2073423.1"
                     /translation="MDTAINLVMIIISVVLIFIILLQTKGSSFSGAFGGGADSIQRTR
                     RGFEKTLFDFTVAIAAFFVVVAVISSFAMS"
     gene            7078..7365
                     /gene="tatA"
                     /locus_tag="VGR29_07250"
     CDS             7078..7365
                     /gene="tatA"
                     /locus_tag="VGR29_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEV2073424.1"
                     /translation="MMGLGWQELAIILVIVIIIFGAGKLPEIGSGLGKGIRGFKEESG
                     LHDDPKKDQAIERGESVTSSTITASARDSRETVAEPVERERRERNIRADEI"
     gene            7541..8293
                     /gene="lepB"
                     /locus_tag="VGR29_07255"
     CDS             7541..8293
                     /gene="lepB"
                     /locus_tag="VGR29_07255"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV2073425.1"
                     /translation="MTEHERQPTPLIVDSRKDSSSPVDASDHQSQQPGTAKATKQTSL
                     IHEVIETAILALLIFVAVRSVVLNFKVDGLSMMPSFENGEMLLVNRNSYRSVDAWDFV
                     DWLPLVDEHEDAEPFITFGEPSRGDVVVFTPPSPGQDKPYIKRVIGLPGDTVEIREDG
                     VSVNRTLINEPYLEGATTSCQSGGASYCGPLTVPEDSIFVLGDNRNNSEDSRFFGPVP
                     ESNIIGKAWITYWPADVIGVVPHYDYPKLPET"
     gene            8476..9063
                     /locus_tag="VGR29_07260"
     CDS             8476..9063
                     /locus_tag="VGR29_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEV2073426.1"
                     /translation="MMIEATVESIRVSLVTQNRVVILKEVDGTRHLPIWIGAYEAEAI
                     ALELQGIVATRPLPYDLIRTMLMELGATIDRIVIADLTDAVFYARIILSVADRTIEID
                     ARPSDAIAIAVRTESRILVEDSVMASAGVSLDDDDFNPTAMQSDTGENISLSDLSGAT
                     SAADETAGAGDQLGVFRDFINSLDLDDFDKGNKAN"
     gene            9147..9521
                     /locus_tag="VGR29_07265"
     CDS             9147..9521
                     /locus_tag="VGR29_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073427.1"
                     /translation="MDSEVQIKNIVLKQMNRCVVCHREFDTDDIRVLSRKPDIWTMLV
                     ECDDCHARNFVAAVLNDGDPKEAQLALRRLTADALAVDETEEAIAPEGPTSGERVSAG
                     DVVDMHQFLDEFDGDFIRLFRS"
     gene            9747..10328
                     /locus_tag="VGR29_07270"
     CDS             9747..10328
                     /locus_tag="VGR29_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEV2073428.1"
                     /translation="MTQPDQTHTAVRPVPAPYTGRQDIDLDVVREMLSHFKYDDFEEA
                     QELILSALQEVNEHFGWVSPEAAEVVAEQLGTTSTRVFALLTFYADFRTAPRGHHHLL
                     VCHGMSCYVMGSQNLVQTLQDDYGIVDGDTTRDAQLSVQVVNGCLGVCDLSPVVKLDD
                     EFAGPVSPESLNQLIQRALAGREAMRANEETHG"
     gene            10321..12051
                     /locus_tag="VGR29_07275"
     CDS             10321..12051
                     /locus_tag="VGR29_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEV2073429.1"
                     /translation="MVNTTPSEHLSRLGSKEEFQAFQQGVVDRWTEMWDGNSTVVSVG
                     VGSSSVAKGALEIFERVRSELGDCATIREVSGNGAFWMEPWIEVKRPGFMPVIYGPLT
                     LSDVAGVLAGAREDLAVGVRSEEAQGDVPPLADHPYFKDQLQLLTRNAGVIEPDSIED
                     AIARGAYQGYFKVLFELSQEEAIAEVTTANVRGRGGGGFPAGIKWESGRKAKNSPKFV
                     VCNSHEGEPNVYKDRHLHEADPHRILEGILIACIACGADRGYNYIGGEYPLGIRRFRK
                     AVADAERLGLIGKNILGTGKNVAFRVRTGGGAYICGEASALMYSVMGVRGMPRTKPPR
                     SVEEGLWKRPTVANNTETLANIPDIIVNGGEWYAKIGTEKSKGTKLMTVQGPMHHHGV
                     VEVEMGMTMNTLFYDVYGGMREGYELKGIQTGGVSAGPITKEHFDRPIDFDSYTDING
                     MLGSGGWVIFDEKVCVVDFARYMTAFSRYESCSKCVPCRLGNPALVEILDRIRFGRGT
                     LEDLALIRRTSKHIIELSLCGLGQVAPVPLIGMLDTYEEEFRRHIVDGVCPTGTCPIS
                     VGRPVLAAAD"
     gene            complement(12228..13253)
                     /locus_tag="VGR29_07280"
     CDS             complement(12228..13253)
                     /locus_tag="VGR29_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HEV2073430.1"
                     /translation="MDEEPTTREDYEMLALSKRDIQNLISMGDAIELVKTAFYELREG
                     RAVVPLRSSVEIAPGSAMLLLMPAYMPGLPALGFKAVSIFQENGRRNVPTANAMVCMI
                     DPQTGVPAAVLNGAYLTALRTGAVSGASAQLMAREDAKNLVVIGAGAQGVTQAAAVCE
                     VRDIDKITVVYRREASWKSYRDAIVEDWPHLRDRLSGTDDVEKAVREADIVCLATTSR
                     TPVFEDAWIKKGTHVSGVGSFTPYMQEVPSDFVARSRVVLDMKEHALEEAGDLIIPLR
                     DGVISEDQIVGELGDVVAGTLMPRENDMDVTFFKSVGNAVQDMAVANAAVLRAQEQGV
                     GQQIDLG"
     gene            13693..15999
                     /locus_tag="VGR29_07285"
     CDS             13693..15999
                     /locus_tag="VGR29_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073431.1"
                     /translation="MAGAGWCTHPKRQLSSDVRILVRPAELACRNAWGVDMWMDKDGI
                     APDAPAAVDQPTAAPAPEVPAPTPVAQVSFDDEITSVVSSEIHRSLTDQDLNDRLVEQ
                     TTILPEERLSSHEADDRFHLLARGGRDSVGQARQRHLRRRQADAPILGGDDNPQTEES
                     GSTLETSATSGFTPDDYVEQETPASKAVEEEPEEPDQPDEDVLLSQDSRPGSPRSRRL
                     RRSRELPQSPKFGTGVAMELDAHALTDLPETGVRDTSDSEAMSQSSTDIDPLPPRCES
                     SPSDTEQPGTGEVEHMLSHGTEENAYDHVMKRTQGIRAAARVEREERVRHEQRRTVAP
                     LGMDDRVERPSTRLGAPIARRVPAGGEDLPLKKAFQENKEPATLLPDPAVEDRGHVRQ
                     RPTAHEYQSGARKANDDRRTSTERWSPTSHRQEARSSWWRGLFNRSDRSTTDDTEGWE
                     ESIDEPYLSSASKDWETGETDSWLNEHESQAAAQYEFPPNASTDEDGERNAAAYEDDP
                     GPTSGRVGAEYERLDLQNEGDMVAFRQRLFTSHCEPGASGVATHTEEPVARAPVALSR
                     QRPAPAEPTPPQNPANGFVKQPMRGAGQGGFRETQHDQELIRGRVSDQFVTRRRSGYQ
                     PQREPERASTNVLPVNSLLGAPADEGFEPEVTPEFDIRAIVEQRTELLDMAITIAPDV
                     PRECSTCRSYRMSEQGERGWCTNNWAFTHRQMVNATDLACQSTIGCWWLPADEEVGLI
                     TDESGYVATPRVDRLIAQLDPLKRAVGR"
     gene            16240..17091
                     /locus_tag="VGR29_07290"
     CDS             16240..17091
                     /locus_tag="VGR29_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2073432.1"
                     /translation="MAGFGETLRQARAQKGVTLREAEQATRINRHHLAGLEEENFGAL
                     PPLIYQRGIVRNYASYLDLDPSKMLAMFEEAHGITATGDSKGVTPPPIDMPSHWAPNF
                     AIIAFAVVLSAIVFAWGYSIYVTPPSEDPVATLVASTTTPLTNNIDIATQLPDAPVQP
                     TVLPTQPPAPTATTEEVAEVSGRQNDQPVPEAPVSENAYVTGITVVAENDIQLTVVVD
                     DQVVYDGVLPAGQMTDQFVGSSFSFTTSSGASTSFINACGDSFEMGNEESGVTYDIQA
                     TENSCRP"
     gene            complement(17162..18091)
                     /locus_tag="VGR29_07295"
     CDS             complement(17162..18091)
                     /locus_tag="VGR29_07295"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV2073433.1"
                     /translation="MTLESGKAHRFTRGDRVVVIANPATRVNTRTVVTILERTVPDGV
                     SLDLQWTRAPGGAKDLAAEALQAAVDGVIAVGGDGTVSDVASALLGTGMPLGVLPGGS
                     TNIVAREHGVPKDMFEAARLIFGRHVLAPADVALCNERPFLHMAGAGFDSLFFNMTDP
                     ALKRRIGWMAYLPAAAQALLVQPATYRIEAEGEMITAKSPMVLVANGASIITPMLKLS
                     PDIRSDDGWLDLIVVTATKPHELASVLARLATLQFEKSPLVVHRRVRTLSLVSSREIP
                     VELDGDVLETTPATFGIVPAAIEFIVPARQQSH"
     gene            complement(18397..18720)
                     /locus_tag="VGR29_07300"
     CDS             complement(18397..18720)
                     /locus_tag="VGR29_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073434.1"
                     /translation="MIQHPQARRRVQEQRSQVALEVLLVLYAVLATIVLIRTVLVLLH
                     ISERIWVGSFIYGLTSPVTDALAVVPGFSRALLGPLTMVDIILLALVVLFPLGIVATG
                     GRASR"
     gene            complement(18717..19940)
                     /gene="coaBC"
                     /locus_tag="VGR29_07305"
     CDS             complement(18717..19940)
                     /gene="coaBC"
                     /locus_tag="VGR29_07305"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEV2073435.1"
                     /translation="MSRREPSAGFRGATIVLGVTGGIAAYKAADLTSNLVQAGADVHV
                     VMTDGGTRFVQPLTFEALTRNRVYTSVFEGWYEDSAGHVTLARNADVVLVAPASANTI
                     AKMAHGRVDDMLGAVLLATEAPIIVAPAMEHHMWHHPATRHNIETLGARGVHIVEPES
                     GHLASGAFGDGRLAGLDRLLAAIRLRLGAGGPLTGRSVVVTAGGTREAIDPVRYLGNR
                     SSGRMGVALAHAAIDAGAQVTLIAGPTLEQVPRDIDMQRVESARDMDAAVQAAASVAD
                     VLIMAAAVTDFRPEEQHDQKLKKRAGEDRLTLELVKNPDIIAGVNGASVLKIGFAAET
                     ENLLHNAAEKLEAKDLTMIVANDAVSTIGHDRVRATLLFRDGRDPLQLPELSKDEAAR
                     CVVDELPALLEGHSP"
     gene            complement(19927..21027)
                     /locus_tag="VGR29_07310"
     CDS             complement(19927..21027)
                     /locus_tag="VGR29_07310"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEV2073436.1"
                     /translation="MAFGETTPHQNGRKVIDLRSDTVTRPTPQMREAMAKAVVGDDQY
                     GEDPTVKRLEQRAAELVGKEAAVYVPSGTMGNLTSVLAHCDRGDEIVIGDESHIFWYE
                     SAGPATVGGVSPRTVRTSRLGTYDLEEVVHAIREPRPGYPRTGLLCVETTYNRCGGVA
                     VPLEHLRVLRDIADRHGIPVHMDGARIFNAAVALGVTPAEIASTVNSVQFCLSKALAA
                     PVGSIVAGEQAFIQRVRRQRKVLGGDMRQSGVIAAAGLVALETMVDRLPEDHRRARML
                     AEGLDAVPGLTVDLATVQSNIVVTKTGPSLDKVTFAMALKDAGVLVSDYGLRGLRMVT
                     HYEISDEDVRSTLEIVSRVASGMVGEPARVPA"
     gene            complement(21160..22530)
                     /locus_tag="VGR29_07315"
     CDS             complement(21160..22530)
                     /locus_tag="VGR29_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2073437.1"
                     /translation="MSFDQPSRRRPPERLADRYQVQEVIGSGGSAITYRGRDERLDRP
                     VAIKILRSHYAQDETFVRRFGREARTAASVSHGNVVDVYDFGQHEDALYIVMQLIEGE
                     DLKHLIEREAPLDSSRVQPIIGQVLDGLQAIHEAGIIHRDIKPQNVLIGRDGIARVTD
                     FGIAQAEEDSGMTTAGTTLGTAAYMAPEQAQAGRIMEATDLYAVGVVLYEMLTGFLPF
                     NAPTPVAIMLEHIRTPPVPPSRRMPGRGITSSMDAVVLQALAKVPEDRFRSARAMKQA
                     VTRAFQGGAYDGQQPTVAIPAAHGTRQLPHQRGSTRPPQGSTYSRVQARPVGPGTAER
                     EGVGLGGAVVGLLLVILLAVAGGAAWFAYDAWRDTQATETIPPTATVPAVVAPTATLA
                     PEATDAVILPPISTDIPLPTETPQSTPTDAPTVTQEPTATDEPVLIEPIDGTPFTITN
                     DGQSRR"
     gene            22669..23892
                     /locus_tag="VGR29_07320"
     CDS             22669..23892
                     /locus_tag="VGR29_07320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073438.1"
                     /translation="MAVVGNLGLDRSGRHILVWMCILIAVNQFGFGAIVPVVALYAED
                     FDVSKTAIGLTIAIYGLARFLVNVPAGRLADLSGRRSTLAAGGAITVVGAVLCAVSPN
                     YTFFLVARFIAGAGAAFVLTGGQIVLADIASPQNRGRVMAIYQGVFIVAVGAGAWPGG
                     WLATNFGLAAPFWANAILAGALTVVAWFFVPETRPRATTASGTVSFVPTATMFQQLHT
                     LSASSGLMMIGAVSLAAAFARTGGLFNVIPLLAEDEMGLTPSQIGLGLGMISILGLVL
                     VYPSGALVDHFGRKAVIVPSMILSGVAMISFAWADTFVTFMVASTFWAAATGIAGAAP
                     AAYAADVAPPGMTASAMGMYRTLSDLGYVIGPLALGAIADLVSPDAALVVTAALLASI
                     GVLFAMNALETLQRA"
     gene            complement(23925..25805)
                     /locus_tag="VGR29_07325"
     CDS             complement(23925..25805)
                     /locus_tag="VGR29_07325"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2073439.1"
                     /translation="MNSPDRSGETPRETARSVVPKPLHRRPEWLHLLRVRLALPFIAL
                     TTLVLIVLYLVISESTERIYIDRLHDELVSQANLVAFSITRAPEVGGSTTELSALIDN
                     LSRLSDARLTVIGPDGDVIADSQADASLMENHNSRPEVIQARRSGSGDAARVSSTVEE
                     RFLYVAVLVPESNGQVLRLAVPLDDVEATVSAAQRSILAAVAFVIVASIIAAWIIAGR
                     LARPLQQLRDQAKGVATGDFSSRVALTGSVEIDAVADAFNTMTAGLELSLQEQEKTSV
                     RLQAIMEGLADGVVLTDENGLVLRMNRSAQALLGTDERSAQGRPFVQVTRDHEMARVL
                     GSALRGKKNPSSTVVFGMERLVILVSARTVEGAHERLGLVVLRDVSELRRLETVRREF
                     VANVSHELRTPLTSIRALVETLEGGVVEEEEMARDFLGRIVGEVNRLNALVEDLLDFA
                     RLEAGRSPLQLETVDLGEVVRAGADRLRTQIDRAELSFVVNVEDDLPQVEIDARRMEQ
                     VLLNLIHNAIKFTPAGGEIVISVWRKKGEIAVEVRDTGIGIAPEELDRLFERFYKSDR
                     ARHSVGTGLGLAIAKNIVQLHGGEITVSSAPGEGSTFTFTLPIRRKRARKRARRHAIG
                     LA"
     gene            complement(25792..26556)
                     /locus_tag="VGR29_07330"
     CDS             complement(25792..26556)
                     /locus_tag="VGR29_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002068740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2073440.1"
                     /translation="MNDAAESPQSACVLVVDDEAIIRDTVTLNLQREGMRVVQAATGP
                     DALARMTEDRPDAVVLDIMLPGMDGLDVCRKIRERSSVPILLLSARGEEIDRVLGLEL
                     GADDYLTKPFAMRELVARVRAMLRRAKMAQNLEYIGRSEHDSLATQATPTVVAGDLSI
                     DVAARQAFLGGRALSLKPKEFDLLLYLARHPGIVMTREALLREVWGYQHRVDTRTVDV
                     HIRWLRQKLENDAALPTRLLTVRGHGYRLVVTDEFT"
     gene            complement(26644..27696)
                     /gene="hemE"
                     /locus_tag="VGR29_07335"
     CDS             complement(26644..27696)
                     /gene="hemE"
                     /locus_tag="VGR29_07335"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEV2073441.1"
                     /translation="MSSTIQVMTGRERFLAAANMQSVDTTPVWFMRQAGRCLPEYREL
                     RMKYAFLEVARTPELAVEATLMPVDRFGVDGAVLFADIMLPLESLGVPFEIKPGVGPV
                     IEHPIRTMEDVEKLRVVGPEEGTPYVLDALRLLRRELDGRAAALGFAGAPFTLACYLV
                     DGRSGREYPATKAMMYGQPDVWHGLMDKLTSVTELYLHGQVEAGADAVQLFDSWLGLL
                     GADAYREFVQPYTTRIFSALKGKTRSIHFSTGTSHLLPVIRDTNPDMVSVDWRIRLDA
                     AWEVIGRDKGIQGNLDPTMLMAPWSVVERAAGDILEQAGNRPGHVFNLGHGILPETDP
                     DQLQRLTEFVHASGGR"
     gene            complement(27736..28743)
                     /gene="hemB"
                     /locus_tag="VGR29_07340"
     CDS             complement(27736..28743)
                     /gene="hemB"
                     /locus_tag="VGR29_07340"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEV2073442.1"
                     /translation="MTLSTTSPIPLPVFQRTRRLRQNNALRAFARETTLSASDFIYPI
                     FVVEGEDIRNEISSMPGQFQLSLDSLAGELNDLKELGIPGVLLFGIPSEKDEIGSGAF
                     AADGIVQRAVRLIRDHAPEMLIVTDVCACEYTSHGHCGIIVSGVVDNDQTLPLLARTA
                     VSHAEAGADIVAPSDMMDGRVAIIRQALDDTGFVNTPIMSYAAKYASAYYGPFREAAE
                     STPTFGDRRTHQMDAGNIREAMREIEIDMREGADAIIVKPALSYLDVLRAARERVDVP
                     VVAYNVSGEYAMIKAAAQLGWIDERRLTLETMLSMKRAGADRIITYHARDVARWLQDE
                     A"
     gene            complement(28740..30491)
                     /gene="hemC"
                     /locus_tag="VGR29_07345"
     CDS             complement(28740..30491)
                     /gene="hemC"
                     /locus_tag="VGR29_07345"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEV2073443.1"
                     /translation="MSGNCVSVSSEPKSFRVGTRGSALALWQAHAVQALLQKASREHA
                     FELEIIRPEGDIDKHSSLMKIGGRGVFTSALQVQLLDGRVDLAVHSTKDLPSLAPNGV
                     TIAAFPEREDARDALVSRHGVSLNELPRNPVIGTSSRRRAVQVLAIRPDAEIRELRGN
                     IDTRLRKGLSEIYDAVILAAAGLRRMGWDDPITALMPVDQFVPAPGQGALAVETRIAP
                     DPAQEIGARLDDADIRLAVGTERAFLRGVGGGCTTPIGAHARVERDRAIPVVRFWGML
                     ASDDGRRLERVYEEFSLDDAETGAFAVANRLVRTVAPKWIGTDHLNPLAGVTVLVTGS
                     EAQGQPLMDALTARGAQAVRMPTITIQPVADTSPVENAVERAVGGEIDWLVLTSAHAV
                     EPLAVHLHGRDVAARIAVVGTRTQEALERAGLKAHLVAAGPGAEQLVREMVHVGVYGN
                     KVVCLVSDKARPVLADGLRAAGADVEAIEAYRNVPVSEVDGEVRSLIHCGKIDAVTFA
                     SPSAIDGFRHLAGADLPALSGAAFFSIGPTTSAALRVAGLPMHGEAPTQDAEGFIHAL
                     QHYFGHAAAVSAPGVHV"
BASE COUNT         6379 a         9660 c         8960 g         6098 t
ORIGIN      
        1 gtggacatcc cggacgtcca ttccggcgcg atcacggctc agtccaccgg ggcccagagc
       61 cgaaagacga cgcttgtgcg tcaactcggc gagcggattc agctggtcca tgaactgcga
      121 gagctgcgag ccaccaaaga attcacgaac tgcagccata actgggcgcg tcgtgctgcc
      181 cagcatctgc ggcgtgaccg tctccggatc ctgcagcgtc atgcgctcac ggataccgcg
      241 ctccaggcgt tggaaaccgg tgcgcacgtg catctggata agctcaccga ctgaacggat
      301 acgacgattg ccgaggtggt cgatatcgtc cggatggctc aggccgttat tcaggcgaat
      361 catctcccga ataatcgcga cgaggtcctc gttccggagc acgcgctcat cggttcgggc
      421 agcttcgtta tccctgtgca ggcggccatt gagcttgtac cggcccaccc gggcaaggtc
      481 ataccggcgc tcgttgaaga acaggttctc cagcatcgcg gaggcattgt ccttcgtcgg
      541 cggatcacca ggacgcaacc gccggtagag ttcgaccaat gcatcgtccc gggtcttggt
      601 gttttctttt tccatcgtcg tggcgatgta acgatggtct tccttggtat cgacatcctc
      661 gaatgccgcg aggagatcct cgtcgttttc gataccgatg gcccgaagca gaatggtcac
      721 cggcatcttg cgcttgcgat caaccttgac tgagatgaca tcccgattgg aggtttcaaa
      781 ctcaagccag gcaccacggt tcgggatcag cttcgccgtg gaaaggtact tgccggtcgc
      841 gggatcctgc gtgcgttcga aatacacgcc cggagatcgg acgagctgcg agacaaccac
      901 gcgctcggcg ccattgataa tgaacgttcc gtccgaggtc atcaccggga aatcaccaag
      961 gaaaataccg tccggtccgg tttccttaat ctctccggtc tccttcatca ccaacttggc
     1021 ggaaacgcga agcggggccg cataggtgat gtcgcgctcg cggcaaaaga cctcggctac
     1081 ccgcggatcc gtcttggcgc gctcctgctc ttcacccttc ggcatgcgcg accacggcgg
     1141 atcaaagcgc ggctcggaga tgtaaagctc catcttccgg tggtgatcgg tgattggcga
     1201 tatctcgtcg agcagctcac gcagcccctc ctcaacaaac cagttgaagg attcgacctg
     1261 aatctgaacg agattgggca tctccagaac tgtgggaatg ctcgaatatg accgtcggtt
     1321 gacgcccaca ttggacagca cctgcctggg accaatatcg gggacaaccg cggtggttct
     1381 tacatcacga tcaacagcca ttcaccgctc ctctagcgtt gcagatcaca ccggcagtca
     1441 ccgatgaaat ccgcctcttg tcagcgcaat agatctcgcc attaaaagcc gaccatgcgc
     1501 gtgatgtata acgggggaat aaagaatcag gaactcgccg ccgactgccg gacaatggag
     1561 tcaagatcgc gcctgccatg cgcaaactcc tgcgcacgcc gaaacgggag gacatacgct
     1621 gcccctcccg gttacccagg tttttctgtg tggtatgctc agccgcacgc gtcgcccagt
     1681 ccaacactct acgctttcgc ccgccactgc cgcagaatgc aacggcagca cccttcgggc
     1741 gaacgtgtag tgtacctgat attccgcaag aaagtcaata gctgtgaacg ggaattctcg
     1801 gcctggctca cgtcacaccc gcgcgggcct atccaccgcc gatgctatac taaagaaacc
     1861 cgcgatgcac cggcgcggtc gtcacccgca cgacgactgt ggagcctcgt gtgtctgaac
     1921 cagccgtctc acccgacatc atcctggact gtcaatgaac tctcgcacgc tgcggatgtg
     1981 gcttcggccc ggcatgatga tcaagcgctg gatcgccctc ttcatgctgg ccaccatcgt
     2041 cactagcctc ggccttgcca tggctcttgc ctggatttat gagaactacg acttccccta
     2101 cacaatccag ccctacgtcg agtggctgac tctgcagccc atcccccatc cctggcgtga
     2161 agtcatcatt attgtgactg gtctcgcctt ccttgggttc actatttggc ggcttagccg
     2221 atcccttatc gcaccgcttc tcgccggtga cacctccggc cgagcgttct cggagatagt
     2281 caatgatcat cgctttggcc ccgccaaggc agaattcaac gtggtcgcga tcggcggtgg
     2341 cacgggcctg agcgcgctcc tccgaggtct caagctccag aacgtgaacc tcactgccat
     2401 cgtcactgtg gcggatgatg gtggcagcac tggccgcatc cgtaatgttt tcaacatgcc
     2461 tgcgcccggc gatatccgga actgtctggt cgccctggcc gataacgaat cattgatggg
     2521 ccggctcttc cactatcgct tcgacaagga aggctccgag ctctccggcc atgcgttcgg
     2581 caacctcttc atcactgccc tcacccaggt gacgggaagt tttgaacagg gagtgatcga
     2641 gtctgccaag gtgttgaacg tccgtggccg cgtcttgccc tcgaccctcg aaaacatcac
     2701 cttgtgtgcc gatctggtcg acggctcccg agtacggggc gaatctgcga ttgctcatga
     2761 caatcctgcg atcagtagaa tttatcttga tcctgacgca ccggacgcct atcggcctgc
     2821 ccttgcggcg atcctgaacg cggacctgat cgtccttggt ccaggcagtc tctacaccag
     2881 cgtgctccca aacctgctcg tgggcggcat ccgggaggcg atcctgtggt ctcgcggcac
     2941 cacggcgtac gtgtgcaacg tcgccaccca acatggcgag accgacgatt tcggatacga
     3001 ggatcacatc gccgaggtgg tcgaatatct gggcgaccga aagctcgact ttgcgattgt
     3061 gaacagcaac gcggcttccg actccgcaat acggccagag tggcaggttt cggccgttca
     3121 ctacgacggc aaggaattcg ttgccgatgg cgtcaagatt gtcgcgggag atgtcgtcaa
     3181 cgaccgcaac cccttacggc acgatcctgt gaagcttgcc gagatcctga ttggcctcgc
     3241 tcgcgatcgt aaggatgaaa ccgctcccct tgaggtcgcg acaacttcct caaccacctg
     3301 accctccccg ggaacgccgg cacgcgtggc acgccgttcg ctgtaaactg cgtggcatca
     3361 cgtcattccg cgacagtgac gacacaatca tctcgaagaa cggatggcca ggaggcacca
     3421 gtggtttaca agatcggtat taacggattc ggccgcatcg gccgtcaagt attcaaggcc
     3481 gtgaccgaag gcggcttcgg cgatctcttt gagatcgtcg cggtaaacga tctcacgagc
     3541 aacgacaccc tggctcacct gctgaaatac gattcaaact acgggtcgtt cgatgccgac
     3601 atctcctcga gcgaagaggg tatcaccgtc gatggcaaac tgctcagggt tttcgaagag
     3661 aaggaacctg gaaacctgcc atggagtgac ctcgggattc acctcgtcat tgagtcgacc
     3721 ggtcgcttca ccaagcccga agcggcccgt gcgcacatcg atgaggccgg cgcgaagaaa
     3781 gtagtcatct ccgccccgtt gaccaccacg gctgattcct cagccggggt agacgatgcc
     3841 attacggtgg tccttggcgt gaacgaggac aggtacgacc caaagacgca caacatcatt
     3901 tccaacgcat catgcacaac aaactgcctg gcccccgtcg ccaaggtgat cagcgatacc
     3961 ttcggcatcg agcatggcat gatgacgacg gtgcactcct atactgccga tcaagtattg
     4021 caggatggac cccacaagga tctccgccgt gctcgtggcg ccgcccagaa tatcgtccct
     4081 acctccaccg gcgcggctcg cgcgctctcg ctggtccttc cggagctgga aggtaagctc
     4141 gacggctttg cgctccgtgt cccgacaccg acggtctcga tcgtcgattt cgttgcaaac
     4201 accacaaggc cggcgaccgt cgatagcgtg aacgaggcat tcaggtccgc gtcgcaaagt
     4261 gacgagatgg tcggcattct cggctactcc gaagaaccac ttgtttcgac cgactacaag
     4321 ggagactccc gctcctccat cgttgatgcg ctttcaacga tggtccaagg cgacaacatg
     4381 gttaaagttg tctcctggta tgacaacgag tggggatatt cgtgccgagt agcggatctt
     4441 gctgccctca tctgcgagca ggggttcgac tcctaggcgc accttggtac tccaacgcat
     4501 ctctcctcaa gtcgcgcatt ggcattggcg gcagggcata acccggttac atgccggcgt
     4561 gtccgccgcc atgcgagctt gatacccgcg cgctcatgag gcaacactgt cctgggagcg
     4621 cgtgatcgtt tggaaggcag acctattttg acgaagcgat cagtggcgtc ggcggacgtg
     4681 cggggcaagc gcgtgctctg ccgggttgac ctcaatgtgc ctcttgaggg cggggcaatc
     4741 ggtgacgata cccgtatccg ttccgccctt ccgaccatcg agtggctgcg cgaccgaggc
     4801 gcgcgtgtcg tgctctgcag ccatcttggg cggccaaagg gtaaggtggt cgaggatctc
     4861 cggcttgcac ccgtcgctga acacctctcc agacttctcg gtcaggaagt gattgcgatt
     4921 ccgactatca caggcaccga cgcattccag gcgattgggt cgatacagga tggggacatc
     4981 gtcctgctcg agaatctgcg cttcgacccg cgggaagaga agaacgaccc ggagtttgca
     5041 gccgagctgg ccgcacttgc ggacctttac atcaatgacg cgttcggagc ggcacaccgc
     5101 gctcacgcct caaccgaagg catcgctcat ctccttcctg cctatcttgg actgctgatg
     5161 caggaggaga tcaatacgct cggcaagctc ctaaccgatc ccgaacgtcc cttcacggcg
     5221 atcatcggtg gcgcaaaggt ctccgacaag atcgcggtat tgcaaaatct cgtgacccgg
     5281 gttgatgccc tgctcattgg aggcggcatg gcgaacacct ttctcctctc ccagggcaaa
     5341 gaggtcggag cctcactggt cgaagaggac cgcgtcggga cggcaaagga cattctcgaa
     5401 accgcccgtc aacgaggcgt cgatgtgtta cttccagatg acgttgtcgt agcgcgtgat
     5461 atcgacgcgc ctgaaggcac ggtcaaaccc gtagggcaaa tatccgcaga tgaggcgatt
     5521 ttcgacattg gccccgatac cgcccaacgc tatgcggaca ccatttcgac gcagaagacc
     5581 atcttctgga atgggccact gggcgtcacg gagaatcccg ccttcagcat gggaactcgt
     5641 gtcgtcgccg aagcggtagc gaattcagtg ggctttaccg tcgtcggcgg tggggattct
     5701 gcgggggcaa ttgaggacct tggatacacc agccgcatcg accacatatc gactggcggc
     5761 ggggcttcgc ttgagttcct ggagagcaga acgctgccgg gaatcgcggc gatccccgat
     5821 gaaggagaag ccgaatgagc acgccgctga tcgtcgggaa ctggaagatg aacacgtcgc
     5881 tcgatgaggc cattacgttg gccaaggctg cggccgcagt tgcggacggc gctggcgcag
     5941 ggacggatgt cggcatttgt ccacccttta cgtggatcgt tcctgtgcgt gacgcggtcc
     6001 ggggatcgtc cctccgtgtt ggtgcccagg attgctcggc gcacgataac ggcgcctata
     6061 ccggggatat ctccgcgaaa atgctcgcgt cgtgttgcac gttcaccctc gtcggtcatt
     6121 ccgagcgacg gtcggtccac ggagaaaccg acgatctctt gcaacagaaa ttgcacgaag
     6181 ccctggaggc agggctggac gtcatcctgt gcgtaggaga atccgccgag gaacggaata
     6241 cgggaattgc cgatcgggta gtgctgcgcc agatcgatgc gacgctcgac cggacaaggc
     6301 ttaccgacac gagtcgcctc accattgcct acgagccagt ctgggcgatt ggaacaggag
     6361 tcgcagccac ggaggcagat gcatcccaaa tggcgaccct gatccgtgaa gatctcgatg
     6421 cccggttcgg ctccagtgcg gaccacatac gcgtcctcta tggaggcagt gcgaacgatc
     6481 gaaacgcgcc ggcattcctg gctgcgaaga atgtcgatgg gttacttgtt gggagtgcaa
     6541 gcctttccgc ggatgtattc gcatcattga ttgagggcgc ccgcatcacc ccgtaaagcc
     6601 gtccggcgtc gtgttgttgc attgatgcaa gggacgccac agtgctaccc tgcggtcgtg
     6661 catatgctgc ccgagagggc gcgtcactta tggttgcgaa acgaaggaat gaactatacg
     6721 tggatacagc gattaatctc gtcatgatca tcatctcggt ggtgctcatc ttcatcatcc
     6781 tgctccagac gaagggctcg tccttcagcg gtgcgtttgg cgggggcgca gattcgatcc
     6841 agcgcacacg ccgcgggttt gaaaaaaccc tcttcgactt cacggtcgcg atcgccgcgt
     6901 tcttcgtggt tgtggcagtc atcagcagct ttgcaatgtc ctgattaggg tatcccagag
     6961 gttcgagcgg gtggcccgtg cggttcacgg gtcgctatac tgagcgtgtt ccgttgaaac
     7021 aatggtcgga acgagtggaa catccacggc gctcccacag caggaggaat tcgacatatg
     7081 atgggtcttg gctggcagga actggcaatt atccttgtta tcgtgattat tattttcggc
     7141 gccggcaaac tccctgaaat tggctcgggg ctcggcaagg gcattcgagg ttttaaggaa
     7201 gagtcggggc tccacgatga tcctaaaaag gatcaagcga ttgagcgggg cgagtccgtc
     7261 acatcatcca ccatcactgc atcggctcgc gattcccggg aaacggtcgc cgaaccggtt
     7321 gagcgcgagc ggcgagagag aaatatccgc gccgacgaga tctagctcgt cagtccagat
     7381 cagagttctg tggagcgctc tcacttcaaa gcggatgtac cgggacggag acctctctcc
     7441 gtcccggtag tttgttaaag agcgtccgca tcgatgaggt gtatcatcgc ccagggactg
     7501 tgtccccaat tgtcaatcgc ccagaaggat gtaccagcgc atgaccgagc acgagcgcca
     7561 gccgaccccc ctcatcgtcg attccaggaa agactcgtcc tcgcctgtcg atgcttctga
     7621 ccaccaatcc cagcagccag gcacagcaaa ggcgacgaag cagacatcac tcatccatga
     7681 agttatcgag accgcaatcc tcgccctcct gatctttgtg gcagtgaggt cggttgtgct
     7741 caatttcaag gttgatgggc tgagcatgat gccgagcttc gagaatggtg agatgctcct
     7801 cgtcaaccgc aactcatatc gatcggtcga cgcgtgggat tttgtcgact ggcttcccct
     7861 tgtggatgag cacgaagatg ccgaaccgtt catcacattc ggcgagccga gccgcggtga
     7921 tgtcgtcgtc tttactccgc cctctcccgg gcaggacaaa ccgtatatca agcgggtcat
     7981 cgggcttccc ggtgataccg ttgagattcg tgaggacggt gtttcggtca accgcaccct
     8041 tatcaatgag ccttatctcg agggagcgac gacgagctgc cagtccggag gggccagcta
     8101 ctgcggcccg ctcacggttc ccgaggactc aatctttgtg cttggggata atcgcaacaa
     8161 cagcgaggat tcgcgcttct ttggccccgt gccagagagc aacattatcg gcaaggcgtg
     8221 gatcacctac tggcccgcgg acgtgatcgg cgtcgtgccc cattacgact acccgaaact
     8281 cccagagacg taaccgcatg ggaccgagca tatgacgacg atcggcgggc ggcagcccgg
     8341 cctactgccc ctcgccgttg taaacttgtg aggtcaccgg agcatcggtg tctgcatcaa
     8401 tactccaccg aaacgggctg gttccagtga tgctgcgtgc gaaacccggt cgaacccaat
     8461 cgcggaaagg gcctgatgat gatcgaagcg acggttgaga gcatccgggt cagtcttgtg
     8521 acccaaaacc gtgttgtgat cctgaaagaa gttgatggaa cccgccacct gcccatctgg
     8581 atcggcgcgt acgaggctga agcgatcgct cttgagctgc agggcattgt cgcaacacgc
     8641 cccctgccat acgacttgat ccgaacgatg ctcatggaac tcggcgccac catcgaccgg
     8701 atcgtcattg ccgatctcac cgacgcggtc ttctacgcgc gtatcatcct ctctgtcgcc
     8761 gaccgtacca ttgagattga cgcacggccg agcgatgcaa ttgccatcgc cgtccgcacg
     8821 gagtcgagaa tccttgtcga ggattcggtg atggcaagcg ccggagtttc gctggatgac
     8881 gatgatttca atcctaccgc gatgcagtcg gacacgggag agaacatttc cctctctgac
     8941 ctcagcggag ccacctcggc ggcggatgag accgcgggag cgggcgatca gctaggcgtc
     9001 ttccgcgact tcatcaactc gcttgacctg gacgacttcg acaagggcaa caaggccaat
     9061 tagcaacgaa cgaaaacgta ggcgcacatc ttgcatcttt acacaccagt acgatcgatc
     9121 tgcgcctgac accagagaaa gacatcatgg attccgaagt tcagatcaag aatatcgtcc
     9181 tcaagcagat gaatcggtgc gtggtctgcc atcgcgagtt cgacaccgac gacattcgtg
     9241 tgctgtcccg caagccggat atctggacca tgctcgtcga atgtgacgat tgccacgcgc
     9301 ggaacttcgt tgccgcggtc ctcaatgatg gcgacccgaa ggaggcccag ctggcccttc
     9361 ggcgactcac cgccgacgct cttgccgtcg acgagacaga agaggctatc gcgccagaag
     9421 gaccgacaag cggcgagcgc gtctccgccg gtgacgtagt tgatatgcac cagttcctgg
     9481 acgagttcga tggtgacttc atccggctct tccggagcta aagagagaat ctggcttacc
     9541 aggcaaaccc gggcgcgatt gcgcccgggt ttttgtttcc cgtccacgga cgcgcatcac
     9601 attccaagcg tgtgcccatt accagtcctc gtatcccggg gaagctttcc ggcgctgcta
     9661 tactttgcca cggtaggaat tggcgaaatg cgcacactgc cacgctgact ttgcaatttg
     9721 aaaggcgtga cagggaggag tgccgagtga ctcaacccga tcagacccat acagcagtgc
     9781 ggccggtccc ggccccgtat acagggcgac aggacatcga cctggacgtc gtacgcgaaa
     9841 tgctctcgca cttcaagtac gacgacttcg aagaggccca ggagttgatt ctctctgctc
     9901 tccaggaagt gaacgagcat tttggctggg tgtcccctga ggcggcggag gttgtagctg
     9961 agcagctggg aacaacgtcg acacgggtct tcgccctgct caccttttat gccgacttcc
    10021 ggaccgctcc acggggtcat caccacttgc tcgtctgcca tgggatgtcg tgctatgtca
    10081 tgggctccca aaacctcgtg caaaccctgc aggacgatta tgggattgtc gatggtgaca
    10141 ccacgcgtga tgcccaattg agcgtccagg ttgtcaacgg ttgcctcggc gtctgcgatc
    10201 tttccccggt cgtaaaactc gatgacgagt tcgctggtcc cgttagcccc gagtcactca
    10261 accagctgat ccagcgcgct ctcgcagggc gcgaagccat gagagcaaac gaggaaaccc
    10321 atggttaaca caacgcccag cgaacatctg tcacgattag gttccaaaga agaattccag
    10381 gcattccagc aaggggttgt cgaccgctgg accgagatgt gggatggcaa ttccaccgta
    10441 gttagcgtcg gtgttggatc gagctctgtc gccaagggcg cgcttgaaat tttcgaacga
    10501 gtccgatccg agcttgggga ctgtgcaacg atccgcgaag tcagcggaaa cggcgcattc
    10561 tggatggagc cctggattga agtgaagcgt cccgggttca tgccggtgat ctacgggcca
    10621 ctgaccttgt ccgatgttgc cggtgtgctc gcgggggcgc gagaggatct tgcggtgggc
    10681 gtgcggtcgg aggaggccca gggagacgtc ccgcccttgg cagatcaccc ctacttcaag
    10741 gatcagcttc aacttctcac ccgtaatgcc ggtgtgatcg agcccgactc catcgaggat
    10801 gccatcgcgc gtggtgccta tcaggggtac ttcaaggtcc tcttcgagct gagccaggaa
    10861 gaagcgatcg ccgaggtaac gaccgcaaac gtccggggac gtgggggcgg aggcttcccg
    10921 gccggcatca agtgggagtc tggacgcaag gcgaagaact caccgaagtt cgtcgtctgc
    10981 aacagccacg aaggtgagcc gaacgtctac aaggaccgtc acctgcatga ggcagacccg
    11041 caccggattc tcgaaggcat cctgatcgcg tgcattgcct gcggcgctga ccgtgggtac
    11101 aactacattg gtggtgagta tccgcttggt atccgccgct tccggaaagc ggttgccgac
    11161 gcagaacggc ttggcctcat tgggaagaat attctcggaa caggcaagaa cgttgcgttc
    11221 cgtgttcgca ccggcggcgg cgcctacatt tgtggcgaag cctcagccct gatgtattcg
    11281 gtcatgggcg tccggggcat gccgcgcaca aagccgcctc gctccgttga ggaaggactc
    11341 tggaagcgcc cgacggtcgc caacaacact gaaactctcg caaacatccc tgacatcatc
    11401 gtcaatggcg gtgagtggta cgccaagatc ggtaccgaaa agagcaaagg caccaagctc
    11461 atgacggtcc agggcccgat gcaccatcat ggtgtcgtcg aggtcgagat gggcatgacc
    11521 atgaacaccc tcttctacga cgtttacggc ggcatgcgtg agggctacga gctcaagggt
    11581 atccagaccg gaggcgtctc cgcaggcccg atcaccaaag agcacttcga tcgcccaatc
    11641 gactttgact cctacaccga catcaatggc atgttgggct caggtggatg ggtgatcttc
    11701 gacgaaaagg tctgtgtcgt cgatttcgcg cgctacatga cggcattcag ccggtatgag
    11761 tcgtgctcca agtgcgtccc atgccggctc ggcaatccgg ccctcgtcga gattttggac
    11821 cgtatccggt tcgggcgcgg cacgctggaa gacctcgcgc tcattcgccg aacgtcaaaa
    11881 cacattatcg aactctcgct ctgcggtctc ggccaggttg caccggtgcc actgattgga
    11941 atgctcgata catatgagga agagttccgt agacacatcg tcgacggggt atgccctact
    12001 ggtacctgcc cgatctcagt tgggcgaccg gtgctggctg cggccgatta gcagtccccg
    12061 tcacgaaatc acagagcgag gccgtggcgt catgccccgg cctcgctact tcaataccga
    12121 acggagaacc tccgggcttc ctatgctaac aagggggccg agttgctcca tggcacgagg
    12181 gctgttgcaa gcaggggtgt gctcaagtca aacctgaacg gcctcgttta accgagatcg
    12241 atctgctgac ccacgccctg ttcctgtgca cggagcacag cagcattggc aacagccata
    12301 tcctgtaccg cgttaccaac ggacttgaag aacgtgacat ccatgtcgtt ctctcggggc
    12361 atcagcgttc ccgccaccac atcgcccaac tcgccgacaa tctggtcctc agagatgacg
    12421 ccatcccgga gagggatgat caggtcccct gcctcctcaa gtgcatgctc tttcatatcg
    12481 aggaccacac gcgaccgtgc tacgaagtca gaggggacct cctgcatata cggagtgaag
    12541 gaacctacac cacttacatg tgttcctttc ttgatccacg catcctcaaa cacgggtgtc
    12601 ctggatgtcg ttgccaggca tacgatgtcc gcctcgcgta ccgctttctc gacatcgtcc
    12661 gtaccggaca accggtccct gagatggggc caatcttcca caatcgcgtc tcgatatgac
    12721 ttccaggagg cctcccgacg atagaccacg gtgatcttgt cgatgtcacg aacctcgcaa
    12781 acggcggcgg cctgtgtcac accctgtgct cccgcgccta taacgacgag gttcttggcg
    12841 tcctctcgcg ccatgagctg ggccgaagca ccggagaccg ctccagtacg cagggcagtc
    12901 aggtacgctc cgttgagcac tgccgctggc acccctgtct gcggatcgat catgcacacc
    12961 atcgcattcg cagttggcac gtttcgcctt ccattctcct gaaagatgga aacggccttg
    13021 aacccgagcg caggcagccc gggcatgtac gccggcataa gcagaagcat cgctgaaccg
    13081 ggggcgatct ctactgagct cctcaatgga acgactgccc gaccctcgcg caactcgtaa
    13141 aatgcagtct ttaccagctc aatcgcatct cccatagata tcaggttctg aatgtcacgc
    13201 tttgagagcg ccagcatctc gtagtcctct ctcgtggtcg gttcctcgtc cacttcgggc
    13261 caccaagttc cgagtgtacg cttgggaagc caccaacacc acacgagcaa tgattgccgc
    13321 atggtgacag agcgacaccg ctgatctctc tattgtccaa ggaatcactc cttagcaaac
    13381 ttctacaatc agcgatacga cccatctttg ccgcccgaac gtactcgttc gtacaacaag
    13441 tacggtcgtt ctgaacgtac ttgttgtgtc ttgtagacaa gctcgaaggg gcactgtaca
    13501 atcggataaa cggggtcaac ccttctctaa cgaccaagga aacgaccaag gacactgcct
    13561 ttcgaattgt ggggattcgg caggttgtgg gacggtgttg accaaaaaac ctctccggcg
    13621 tagcaaaagg tctcaaatat gtcaggaaag aaacggcaat gtcttacctg ccggtacttc
    13681 caggatgcag gcatggcggg ggccggatgg tgcacccatc ccaaacgtca actttccagc
    13741 gatgtacgca tcctggtgcg tcctgctgaa cttgcctgcc ggaacgcgtg gggcgtggac
    13801 atgtggatgg ataaggacgg cattgcaccg gatgctccag cggcagtcga ccagcccact
    13861 gcagcccctg ctcctgaggt tcccgctcct actccggttg cacaagtgtc cttcgatgat
    13921 gagatcacat cggtcgtcag ttcggagatt catcgctcac ttacggatca ggatctgaac
    13981 gatcgactcg tggagcagac gaccatcctg ccggaggagc gtttgagcag ccacgaagcg
    14041 gacgatcgat tccatctcct ggcacgaggt ggacgagact ctgttggaca ggcacgacag
    14101 cgccatctgc ggcggcgtca ggcagacgcg ccgatccttg ggggcgatga caatcctcaa
    14161 acagaggaat cgggatccac gctcgagacc agcgccacca gcggcttcac accggacgac
    14221 tacgttgaac aggagacacc tgcgtcgaag gcagtggagg aggaaccgga ggaaccggac
    14281 caacccgacg aggatgttct gctcagtcaa gacagcaggc cggggtctcc tcgcagccgc
    14341 cggcttcgac ggtcgcggga gttaccccaa agcccaaaat tcggcaccgg cgtcgcgatg
    14401 gaacttgacg cgcacgccct gaccgacttg cccgaaaccg gcgtacgcga cacgagcgac
    14461 tctgaggcca tgtcgcagtc ctccaccgac atcgatccat taccaccacg atgcgaatcc
    14521 tctccttccg acacggagca gccaggaact ggtgaggtgg agcatatgct gtcgcatggc
    14581 accgaggaaa acgcctatga ccacgtgatg aagcgcaccc agggcatcag ggctgccgca
    14641 cgcgttgagc gtgaggagcg ggtgcgtcac gaacagcgca gaaccgttgc cccacttggc
    14701 atggacgacc gagtagaacg tcccagcacg cgtctcggcg caccgatagc gcgacgcgta
    14761 ccggcaggtg gcgaggatct gcccctgaag aaggcgttcc aggaaaacaa ggagcctgcg
    14821 acgttgcttc ccgatcccgc cgtcgaggat cgaggtcatg tacgccagcg accgactgca
    14881 cacgaatacc agtcaggggc ccgaaaagcc aatgacgatc gaagaacctc gaccgagcgc
    14941 tggtctccaa catcgcacag gcaggaagca cggagcagct ggtggcgagg cctcttcaac
    15001 cgctccgacc gctcaactac tgatgatact gagggctggg aagagagcat cgacgagcca
    15061 tacctttcat ccgcttcgaa ggattgggag acaggcgaga ccgactcctg gctcaatgag
    15121 cacgagagcc aggctgcggc tcaatatgag ttccctccca atgcttcaac ggacgaggat
    15181 ggggagcgga atgcggccgc ctatgaagac gaccctggac ctacctccgg tcgcgttggt
    15241 gctgaatatg aacgcctcga tctccagaac gaaggagata tggtcgcctt tcgccaacgg
    15301 ctcttcacgt cacactgtga accgggggca tctggcgtcg ctacccacac agaggagccc
    15361 gttgctcgtg ctcccgtcgc gctatcacgc caacgcccgg cacccgctga acctacgcca
    15421 ccacagaacc ctgccaacgg ctttgtgaag cagcctatgc gaggcgcagg acagggggga
    15481 tttcgtgaga cgcagcatga ccaggaattg atacgcggac gcgtgtccga tcaattcgtc
    15541 acgaggcggc gatccgggta tcagccgcaa cgggagccgg aaagagctag taccaatgtg
    15601 ctccccgtga acagtctcct cggtgcgccg gccgacgagg gattcgagcc ggaggtgacg
    15661 ccggagttcg acatacgtgc aattgtcgag cagcgtaccg agctgctcga tatggcaatc
    15721 actattgcac cggacgtccc acgcgaatgc tcaacttgcc gaagctatcg aatgtcggag
    15781 caaggtgaac ggggctggtg tacgaataat tgggcgttta cccatcggca aatggtgaac
    15841 gcaacagatc tcgcctgtca atccaccatt ggatgttggt ggctcccggc agacgaagag
    15901 gtgggactca tcacggatga atccggttat gtcgcaacgc cgcgcgtgga ccggttgata
    15961 gcccagctcg atccgctcaa acgtgcggtt ggcagatagc cctcgccgtc tatgtacgga
    16021 cgggaaatat cttttttctg tgccaaattg gcgcaaatgg gagtgggttc acggtagact
    16081 attggctgca agtatcgcgc gtcttttatt agacacgcaa ggttgagtat cacactcttg
    16141 tgaattattg cagggtacat ggctggagcc ctgccgcctg acatgcttgc tcgcacaccg
    16201 atcgattgaa cgacgtgaga gggagccagg ggaccaccta tggctggatt cggggaaacg
    16261 ctacggcagg cacgcgccca gaagggtgtg acgcttcgcg aggctgaaca ggcgacgcgg
    16321 attaatcgcc atcatctcgc tggtttggaa gaagagaatt ttggcgccct cccaccgctg
    16381 atttatcagc ggggtattgt gcgcaactac gcatcctatc ttgaccttga cccaagcaag
    16441 atgcttgcca tgttcgagga ggcccacggc atcacggcca ccggcgacag caagggggtc
    16501 actccgcctc cgatcgacat gccaagtcac tgggcaccga acttcgcgat catcgcattc
    16561 gcggtcgttc tcagtgcaat cgtgttcgcc tggggttaca gcatatacgt cacccctccg
    16621 agcgaagacc ccgtggctac cttggtggcc tctaccacca ctcctctcac caacaatatc
    16681 gatatcgcta cacagcttcc cgacgccccg gttcaaccca ccgtcctccc gacccagccc
    16741 ccagccccca cagcaaccac cgaggaagtt gccgaggtga gcggtcgcca gaatgaccag
    16801 cctgtacccg aggcaccggt ttccgagaac gcctatgtca cagggatcac cgtcgtggca
    16861 gagaacgaca tccagttgac agtcgtcgtc gatgaccagg ttgtctatga tggtgtcctt
    16921 ccagccggcc agatgacgga tcaattcgtg ggttccagct tctcgttcac cacctcgtcc
    16981 ggcgcgagca ccagcttcat caacgcgtgt ggtgattcct tcgagatggg aaatgaggaa
    17041 tcgggagtca cctacgacat tcaggcgacg gagaactcgt gtaggcccta gcagggaaca
    17101 gaaatagaga cagatgagtg agacccgaca gtaaactgtc gggtctcact catcagatca
    17161 cttagtgact ctgctgcctg gcaggaacga taaactcaat cgctgcaggc acaataccaa
    17221 atgtcgccgg agtggtctcc agcacatctc cgtccaactc tacgggtatc tctctcgacg
    17281 acaccagcga aagagttcgg acgcgccggt gaacaacaag cggtgacttc tcgaactgga
    17341 gtgtcgcgag ccgtgccagc acgctcgcca actcatgcgg cttggtcgca gtgaccacaa
    17401 tgaggtccag ccagccatca tccgaccgta tatcagggga gagcttgagc attggcgtga
    17461 tgatcgacgc gccattcgca acgaggacca tcggcgactt ggcagtgatc atctcacctt
    17521 ccgcctcaat ccggtaagtc gcaggctgga ccagcagtgc ctgagcggca gctggtaagt
    17581 acgccatcca cccgattctt cgtttgagcg ccggatccgt catattgaag aaaaggctgt
    17641 cgaaccctgc tcctgccata tggagaaagg gccgttcgtt gcagagcgcc acgtccgccg
    17701 gggccagcac atgcctgccg aagatcagcc tcgctgcctc gaacatgtcc tttgggacgc
    17761 cgtgttcgcg cgccacgata ttggtcgagc caccgggcag aacgcccaag ggcatgcctg
    17821 tacccagtaa cgccgaggcg acatcggaaa ccgtgccgtc accgcccacg gcgatgacgc
    17881 cgtcgacagc cgcttgcaga gcctctgcag ccaagtcttt ggcaccaccg ggagcacgcg
    17941 tccactgaag gtccagcgac accccatccg gcacggttcg ctcaaggatc gttactacag
    18001 tgcgggtgtt gacacgggtt gcagggttgg caatcacgac gacccggtcg cccctggtaa
    18061 atcggtgtgc cttcccgctc tcaagggtca catgtgcccc tatttacctc tgggcatcga
    18121 cagggacgta tctccctgtc gatgcccaga ctccaggacg cgcatacgtg aagagacgct
    18181 tatgccgatt cttcgtcggg accaaacacc ttgtcgacca gataattggc cagccatgtg
    18241 gccgcagccg tcaataccaa tccgaggatg ccgcgcgtcg tgttcttcaa catacagtcc
    18301 gttcctccca aattgcgtgt ttcaattcaa atgcgtcccc cgaggctacc tcgatggcac
    18361 atccggtcca gtgtgcccca cgaactgcct cgcccgctag cgggacgctc gccccccggt
    18421 cgcaacgatc ccaagtggga acagcacgac cagcgccagc aagataatgt cgaccatcgt
    18481 tagcggccca agaagggcgc gactgaaacc aggcactact gccagggcat ccgttacagg
    18541 agaggtcaga ccatagataa aggacccaac ccagatgcgc tcgctgatgt gcaacagcac
    18601 cagtacagtc cgaatgagca cgatggttgc cagcaccgca tacagcacga gaaggacttc
    18661 aagtgcgact tgcgatcgct gctcctgcac acggcggcgt gcttgcggat gctggatcac
    18721 ggcgagtgac cctcgagtaa cgcgggtagt tcgtcgacga cgcagcgtgc agcttcatcc
    18781 ttgctcaact cgggaagttg aagaggatct cgaccgtccc ggaagagcaa tgtcgccctc
    18841 acccgatcgt gcccgatcgt tgacaccgca tcattggcaa caatcattgt caggtctttc
    18901 gcctcaagct tctccgccgc gttgtggagc agattttctg tctcggctgc gaaacctatc
    18961 ttcaggactg aagccccatt gacaccagca atgatgtcgg gattcttgac gagttcgagc
    19021 gtcagacgat cctcaccggc gcgtttcttc aacttctgat cgtgctgttc ttccgggcga
    19081 aagtccgtta cggcggcggc catgatcagc acatctgcca ccgaagccgc cgcctgcacc
    19141 gccgcgtcca tgtcgcgggc cgattctacg cgctgcatgt cgatatcgcg gggcacctgc
    19201 tcaagggtgg ggcctgcgat caacgtgacc tgcgcaccgg cgtcgatcgc tgcatgggca
    19261 agggcgacgc ccattcgacc cgacgagcga ttccccaaat agcgcacggg atcaattgcc
    19321 tcccgcgttc caccggcggt tacaacgacc gaacgtcctg tcagagggcc gccagcaccc
    19381 aaccgtaacc ttatcgccgc cagcaatcga tccaggcccg cgaggcgccc atcgccaaag
    19441 gcaccggagg caagatgccc gctctccggc tcgacgatat gcacgccgcg ggcgcccagg
    19501 gtctcaatgt tgtgacgcgt ggccggatgg tgccacatgt gatgctccat agcgggcgcc
    19561 acaatgatgg gtgcctcggt tgccagcagc accgcaccca gcatgtcatc gacccggccg
    19621 tgggccatct ttgcgatcgt gttcgctgac gctggtgcga ccaacaccac atcggcatta
    19681 cgagcgaggg tgacatggcc ggctgaatcc tcgtaccagc cctcgaagac agacgtgtac
    19741 acgcggtttc gggtgagcgc ttcgaaggtc agcggttgca caaaccgcgt gcccccgtct
    19801 gtcatcacta catggacatc tgcacccgcc tgcacgaggt tgctggtcaa gtccgccgcc
    19861 ttgtaggcgg cgataccacc cgttacgccg agaacaatcg tcgccccccg gaaccccgcc
    19921 gaaggttcac gccgggacac gcgccggttc ccctaccatg ccagacgcca cccgggagac
    19981 gatctcaagc gtgctcctca catcctcatc cgagatctca tagtgcgtga ccatgcgaag
    20041 tccacgaagg ccgtagtcgg ataccaagac accggcatcc ttcaatgcca tggcaaacgt
    20101 taccttgtcg agtgaggggc ctgtcttggt gacaacgatg ttgctctgta cagtcgcgag
    20161 gtcgacagtg agcccgggaa cggcatcaag cccctcggcc aacatccggg ccctccgatg
    20221 atcctccggc agcctgtcaa ccatcgtctc aagagccacc aggccggccg cggcgatcac
    20281 tccggattgc cgcatgtccc cgcccagcac cttgcgctgc cgccgcaccc gctggataaa
    20341 ggcttgttcg cccgccacga tggaaccaac cggagctgcc aatgccttgg aaagacagaa
    20401 ctgcacggag ttcaccgtgc tggcgatctc tgccggagta acaccgagag caacagccgc
    20461 attgaagata cgtgcaccat ccatatgcac cggaatcccg tggcggtccg caatatctct
    20521 cagtacacgc aaatgctcca gtggtaccgc gacaccaccg caacggttgt aggtcgtctc
    20581 gacacagagc aaaccggtcc tcggatatcc aggacggggc tcccggatag cgtgcacgac
    20641 ttcctccaga tcgtaggtcc ccaaccggga ggttcgaacc gtccggggag agacaccccc
    20701 cactgtcgct ggaccagcgc tctcgtacca gaagatgtgg gattcgtcac caataacaat
    20761 ctcgtcgccg cggtcacagt gggccaacac tgaggtgagg ttgcccattg taccgcttgg
    20821 cacgtacacc gccgcttcct tgcccacaag ctcggccgcc cgttgctcaa ggcgcttcac
    20881 cgttgggtct tcaccatact ggtcgtcgcc gacaaccgct ttggccatcg cctcgcgcat
    20941 ttggggcgtc ggccgcgtca cggtatcaga gcggagatcg atcaccttcc gtccgttctg
    21001 atgtggcgtt gtctctccaa aagccatgat ctctctccat gatggggtat cgatacgaac
    21061 ctgacgagcg gaaggaatta tcagctaatg ctcttcgtat cgtccttgat gtctttcatc
    21121 ctatccctct cgtcctgacc attatcttcg ctgtcaccac tagcgcctac tctgtccatc
    21181 gttcgttatc gtgaagggcg tgccgtcgat gggctcgatc aggactggct catctgtcgc
    21241 ggtcggttcc tgcgtcaccg tcggcgcgtc tgtcggtgtt gattgcggcg tctccgtcgg
    21301 tagtgggatg tcggttgata tcgggggcag gatcactgca tcggtcgctt ccggtgccag
    21361 cgtcgccgtc ggtgccacca ccgccggcac tgtcgctgtc ggagggatgg tctccgtcgc
    21421 ctgcgtatcc ctccaggcat cataggcgaa ccaggcggcg ccaccggcca cggccagaag
    21481 aatcaccaag agcaatccga cgactgcccc accgagccct actccctccc gttcagccgt
    21541 tcctggacca accggccggg cttgcacacg gctataggtc gatccctgtg gaggccgggt
    21601 cgagcccctc tggtgcggga gctgcctggt cccgtgagcg gcagggatgg caaccgtggg
    21661 ttgctgtcca tcgtaagcac cgccctgaaa tgctcgggtc accgcttgct tcatggctcg
    21721 cgcggagcga aaccggtcct cagggacctt ggctaatgct tgcagaacga cggcatccat
    21781 cgacgatgtg atcccccgcc ccggcattct ccgggatggt gggacaggcg gtgtcctgat
    21841 gtgctccagc atgatcgcga cgggggtcgg cgcattgaat ggcaagaaac cggtaagcat
    21901 ctcgtaaagc acgactccaa ccgcatagag atcggtcgcc tccatgatgc ggcccgcctg
    21961 cgcctgctca ggtgccatgt atgctgcagt cccgagggtg gtcccggcgg tcgtcatgcc
    22021 agaatcttct tccgcctgcg caatgccgaa atccgtcacc cgggcgatac cgtcgcggcc
    22081 gatcaagacg ttctgcggct tgatatccct gtggatgatc cccgcttcgt ggatggcctg
    22141 gagcccgtcc agcacttggc cgatgatcgg ctgaacacgc gacgaatcga gcggcgcctc
    22201 acgctcgatc aagtgcttga gatcctcgcc ttcgataagc tgcataacga tatagagcgc
    22261 atcctcgtgc tgcccgaagt catagacatc gaccacgttt ccgtgggaaa cggaggccgc
    22321 cgtccgcgcc tcccgcccga atcgccgaac gaacgtttcg tcctgggcgt agtgagatcg
    22381 caggatcttg attgcgaccg ggcggtccag gcgttcatcc cttccccggt acgtgatcgc
    22441 gctgccgccg gaaccaataa cctcctggac ctgataccga tcggccagcc gctcgggcgg
    22501 acgtctgcga gaaggctgat caaacgacac actactcccc ttcgggtttg gttcccgcga
    22561 gacccgcgaa cacgccccgg cacttgcttc atgtcgctca tgatacgaga ccacggcatc
    22621 ttgcgcggtt catgagccga tgcgatgctg cacccgaggg ggattggtgt ggcggtagtc
    22681 ggcaatcttg ggctggatcg atccgggcga cacattctgg tctggatgtg catcctgatc
    22741 gccgtaaatc aattcgggtt cggcgcgatc gtgcccgtcg ttgccctcta tgccgaggat
    22801 tttgatgtta gcaagacggc catcggcctg acgatcgcta tctatgggct cgcacgcttt
    22861 ctggtcaatg tcccggccgg acgcctcgcg gatctctcgg gacgccgctc tactctggca
    22921 gccggtggcg ccattacggt tgtcggggcg gttctctgtg ctgtctcccc taactacaca
    22981 ttcttcctcg tcgcccggtt tatcgctggg gcaggtgccg cctttgtcct cactggtggc
    23041 cagattgtcc ttgcagatat cgcttcaccc caaaatcgcg gccgcgtcat ggcgatctac
    23101 cagggcgtct tcatcgttgc ggtcggagcg ggagcgtggc cggggggatg gttagccacc
    23161 aacttcggcc tggccgcacc cttctgggcg aatgccatcc ttgcaggtgc cctaaccgta
    23221 gtggcctggt tcttcgtgcc ggaaacccgc ccacgtgcca ccaccgcatc tggaacagtc
    23281 tcgtttgtgc caaccgcgac gatgttccag caacttcaca cgctaagcgc gtcttccggc
    23341 ttgatgatga ttggcgccgt cagccttgct gctgccttcg cccggactgg cggcctgttc
    23401 aacgtcatcc cgcttcttgc agaggacgag atgggactca cgccgagcca gataggcttg
    23461 ggcctcggca tgatttcaat cctgggtttg gttctggtct atccatcggg tgcacttgtc
    23521 gaccatttcg gtcgcaaagc ggtgatcgtc ccgtcgatga tcctctccgg cgtagccatg
    23581 atctctttcg cctgggcgga taccttcgtg acattcatgg tcgcatctac cttttgggca
    23641 gcggcgaccg ggattgccgg ggcagccccc gcagcctacg ccgcggatgt cgccccacca
    23701 gggatgaccg cgagcgctat gggcatgtac aggacgcttt cagatttggg gtatgtgatc
    23761 ggccccctgg cgcttggagc aatcgccgat ctggtttcac ccgacgcggc gctcgtcgtc
    23821 actgctgcac tcctcgccag catcggtgtg ctcttcgcca tgaacgcgct ggaaaccctg
    23881 cagcgagcgt gacggacagt actcttggaa gggcacccgt cgtctcaggc gagcccaatc
    23941 gcatggcggc gggccctctt ccgggccctc ttccggcgaa tgggaagggt gaacgtgaag
    24001 gtagaacctt ctccaggtgc gcttgagacg gtgatctcgc cgccgtgcag ctggacgatg
    24061 ttcttcgcga tcgccagccc caaacctgtt cccacagagt gccgtgcccg gtcagacttg
    24121 tagaaccgct cgaacaggcg atcgagttcc tcgggggcaa tgccgatgcc agtatcccgc
    24181 acttccacgg caatctcccc tttcttgcgc cagaccgata tcacaatttc tccacccgcc
    24241 ggggtgaatt tgatggcgtt gtggatcagg ttgagcagga cctgctccat gcgcctggca
    24301 tcgatctcaa cctgcggcag gtcgtcctcg acattgacaa cgaacgacag ctcggcccga
    24361 tcgatctgcg tccgcaatcg atcagctcct gcccgcacca cctcaccgag atcgactgtt
    24421 tccaactgca gtggcgaccg cccggcttcc agacgggcga aatccaggag atcctccaca
    24481 agggcgttca gccggttgac ctcaccgacg atcctgccca ggaaatccct ggccatctcc
    24541 tcttcctcga ccacgccacc ttccagcgtt tccaccaggg cgcggatgga cgtcagcggt
    24601 gtccgcagtt catgcgagac gttcgcgaca aactcccggc gcaccgtctc cagccgcctc
    24661 aactctgaga catcgcgaag caccacgagc ccgagccgct cgtgtgcacc ttccaccgtc
    24721 cgcgcggaca caagaatgac gaggcgttcc atcccaaaaa cgaccgtcga gctgggattt
    24781 ttcttgcccc tcagcgcaga tcccaacacc cgcgccatct catgatcccg tgtgacttgg
    24841 acaaagggcc ggccctgagc ggaccgctca tctgttccca agagagcttg cgcggaacgg
    24901 ttcatgcgaa gcacgagccc attctcgtcg gtgagcacga caccatccgc caacccctcc
    24961 atgatcgcct ggagtctgac actcgtcttc tcttgttcct ggagcgatag ctcgagaccg
    25021 gcagtcatcg tgttgaaggc atccgcaaca gcatcgatct ccaccgatcc cgtcagtgcg
    25081 accctcgagg agaaatctcc tgtggcaaca ccctttgcct ggtcgcgtag ctgctgcagt
    25141 gggcgggcaa gccggccggc aatgatccat gcggcgatga tgcttgcaac aatgacgaac
    25201 gcgaccgccg ccaggatgga gcgttgcgct gctgatactg ttgcctcgac atcgtccagg
    25261 ggcactgcaa gtcgcagcac ctggccattg gactccggca ccaacactgc cacgtaaaga
    25321 aagcgttcct ccacggtgga actgacacga gcagcatcac ctgaaccgga gcgccgcgcc
    25381 tgtatgacct cgggccggga gttgtgattc tccatcaggg aggcatccgc ctgggagtcc
    25441 gcgattacgt cgccatcggg cccaatcaca gttagacggg catcgctcag ccggctcaga
    25501 ttatcgatta gcgcggaaag ctccgtcgtc gagccgccaa cctcgggagc gcgggtaata
    25561 ctgaacgcca cgagattcgc ctggctgaca agctcgtcat gcaggcggtc gatgtagatt
    25621 cgctcggtgc tctcgctgat caccaagtag aggacgatca gcaccaatgt ggtgagggct
    25681 atgaagggaa gggcgagccg aacccggagt aggtgcagcc attccggcct ccggtgcaga
    25741 ggtttcggca ccaccgaccg tgccgtctct cgtggcgtct ccccggaccg gtcaggtgaa
    25801 ttcatcggtc acgacaagtc ggtatccgtg cccccgcacc gtcagcagcc gggttggcaa
    25861 agcggcatcg ttctccagtt tctgacggag ccagcgaata tgcacgtcga cggtgcgtgt
    25921 atcgacccgg tgctggtacc cccatacctc gcgcaggagg gcctcacgtg tcattacgat
    25981 ccccggatgg cgggcgaggt agagcaggag atcgaactcc ttgggtttga gggaaagggc
    26041 ccggccacca agaaatgctt gccgggccgc cacatcgatg gacaggtcac ccgcgacgac
    26101 agtaggtgtg gcctgagtcg ccaatgagtc atgctcgctg cgaccaatgt attccaaatt
    26161 ctgggccatc ttcgcccggc ggagcattgc tcgcacccgt gcgaccagct cccgcatggc
    26221 gaacggcttg gtcaggtagt cgtctgcacc aagctccagc ccaagcaccc gatcgatctc
    26281 ctcgccacgg gccgacagca acaagattgg aacagacgaa cgctcccgga tcttccggca
    26341 gacatcgagt ccgtccatac cgggtagcat gatgtcaagc actacggcat cgggacggtc
    26401 ctccgtcatc ctcgccaacg cgtcggggcc ggtggcagcc tggacgaccc gcatcccctc
    26461 acgctgcagg ttaagggtca cggtgtcgcg aatgatggct tcatcgtcca ccacgaggac
    26521 acaggcagat tgaggagact ccgcagcatc attcacgggt gtacccccgc atcgggaata
    26581 tctatgcagg aagaggtctt gccgtcgggc cgatgcggca aagtatagcg ggatgccgcc
    26641 ccctcacctg cccccggacg cgtggacgaa ctcagtgaga cgctgaagct ggtcagggtc
    26701 tgtctcggga aggatgccat ggccgaggtt gaagacgtgc ccggggcgat ttccagcctg
    26761 ctccagaata tcgcccgctg ctcgttcaac gacagaccag ggagccatca gcatcgtcgg
    26821 gtccagattg ccctgaatgc ccttgtcccg tccgattact tcccatgccg cgtcgaggcg
    26881 aatccgccag tccacgctta ccatatccgg gttcgtgtcg cggataacgg gcaggagatg
    26941 cgaggtgccg gtgctgaagt ggatcgagcg ggtctttccc ttcagggccg aaaagatgcg
    27001 cgtggtgtag ggctggacga actcacggta ggcatccgca ccaagcagac cgagccacga
    27061 atcaaaaagc tgcacggcgt cggcaccagc ctctacctgg ccgtggaggt agagttccgt
    27121 gacactcgtc agcttgtcca taagcccatg ccagacgtct ggctgcccgt acatcatggc
    27181 cttcgtcgcc gggtactcgc ggcccgagcg cccatccacg agatagcagg caagcgtgaa
    27241 cggcgcacca gcaaatccca acgcggccgc acgtccatca agctcccgtc gcagaagccg
    27301 tagtgcatcc agcacatacg gggtgccttc ctctggcccg accacccgca gcttttcaac
    27361 gtcctccatc gtccggatcg gatgctcgat cacggggccg acaccaggct tgatctcgaa
    27421 cggcactccg aggctctcga gtggcagcat gatgtcggcg aagagcacgg cgccatcgac
    27481 accgaagcga tccaccggca tgagcgtggc ctcgaccgcc agctccggcg tacgggctac
    27541 ctcgagaaaa gcgtatttca tgcgcagctc ccggtactcg ggcaggcatc gtcccgcctg
    27601 ccgcatgaac cagacgggag tcgtgtcgac cgactgcata ttcgccgcgg ccagaaatcg
    27661 ttcgcggcca gtcataacct gaatcgtgct agacaaagcg aattcctcgt ttcaggattg
    27721 ctccaacctg cggcattacg cctcgtcctg gagccatcgg gccacatccc tggcgtgata
    27781 ggtgataatt ctgtcggcac cggcgcgctt catcgagagc atcgtttcca gcgtcaaacg
    27841 ccgctcgtcg atccacccaa gctgggcggc tgccttgatc atcgcgtatt cgccggaaac
    27901 gttgtaggcg acgaccggca catcgacccg ctcacgtgcc gcgcgcaaca cgtcaaggta
    27961 ggagagcgcg ggctttacga tgatcgcgtc tgctccctcc cgcatatcga tctcgatctc
    28021 tcgcatcgcc tcgcggatgt taccggcgtc catctggtgc gttcgccggt ccccgaacgt
    28081 cggggtactc tcggccgcct cgcgaaacgg gccgtaatag gccgaagcgt acttggcggc
    28141 gtaggacatg attggggtat tcacaaaccc ggtatcatcc agtgcctgcc gaatgattgc
    28201 cacacggcca tccatcatgt ctgacggtgc aacgatgtcc gcgcccgctt ccgcatggct
    28261 caccgccgtc ctcgcgagga gtggcagcgt ttggtcgtta tcgaccacgc cactcacaat
    28321 gattccgcaa tggccatgac tcgtgtattc acacgcgcag acgtctgtga cgatcagcat
    28381 ctcgggcgca tggtctcgaa tcagtctcac ggccctctga acgataccat cggctgcgaa
    28441 tgccccagag ccgatctcgt ccttctctga tgggatgcca aagaggagca cccccggaat
    28501 accgagctcc ttgaggtcgt tgagttctcc ggccaacgaa tccagcgaga gctggaactg
    28561 tcccggcatc gaggagatct cgttgcggat gtcctccccc tccacgacga agatgggata
    28621 gatgaagtcg cttgcagaga gggtggtctc cctcgcaaag gcacgcaacg cattgttctg
    28681 ccgaagccgg cgtgtgcgct gaaacaccgg cagtgggatt ggagacgttg tggaaagagt
    28741 catacgtgta ctccgggggc agaaaccgcg gcggcgtggc cgaagtagtg ctgcagggca
    28801 tggatgaaac cttcagcgtc ctgggtcgga gcctctccat gcattggtaa accggcgaca
    28861 cgcagagccg ccgaggttgt cggcccgatt gagaaaaacg ctgccccgga gagcgccgga
    28921 agatcagcac cggcgaggtg acggaaaccg tcgatagccg acggtgatgc gaaggtgaca
    28981 gcatcaatct tgccgcagtg gataaggctc cgcacctcgc cgtcaacctc ggaaaccggc
    29041 acatttcggt aggcctcaat cgcctcaaca tcagcgcctg ctgcccgcag cccatccgcg
    29101 agaaccggcc gcgccttgtc ggagaccagg cacaccacct tgttgccgta gacaccaaca
    29161 tggaccatct cccggaccaa ctgctccgca cccggtccag cggcaacgag atgtgccttg
    29221 agccctgccc gttctagtgc ttcctgagtg cgtgtaccga cgaccgcgat ccgagccgcc
    29281 acatcccttc catgcaggtg cacggcaagt ggctccaccg catgagcgct tgtcagcaca
    29341 agccaatcaa tctctccacc aacagcccgt tcgacggcat tttcaactgg cgaggtgtca
    29401 gcaaccggct ggatcgtgat ggtgggcatt ctcaccgcct gcgcacctct cgctgtcaat
    29461 gcatccatca agggctgccc ctgggcctcg gagcccgtca cgagcaccgt cacaccggcg
    29521 agcggattca gatgatctgt gccgatccac ttgggcgcca ctgtgcggac aagccggttg
    29581 gccacggcaa acgctcctgt ttccgcgtca tcaagggaaa actcctcgta cactcgctcc
    29641 aggcgtcgcc catcgtccga cgcaagcatc ccccagaacc gaaccaccgg gattgcacgg
    29701 tccctttcga cccgtgcatg cgccccgatc ggggtggtgc atccgccgcc tacaccacgg
    29761 agaaaagctc gctcggtccc taccgccagc cggatgtcgg catcgtcgag gcgtgcgccg
    29821 atctcctggg cgggatcggg agcaattcgt gtctccaccg caagcgctcc ctgaccaggc
    29881 gccgggacga actggtcgac cggcatgagc gcagtaatgg ggtcatccca gcccatccgg
    29941 cgcagacctg ccgccgcaag aatgaccgcg tcgtaaatct cggacagccc ttttcgcaat
    30001 cgtgtgtcaa tgtttccacg gagctcgcgg atctcggcat ccggacggat cgcaagcacc
    30061 tgcaccgccc gccggcgtga agacgtgccg atcacaggat tccggggcag ctcattcagt
    30121 gatacgccgt gcctggagac gagggcgtca cgtgcgtcct cccgctccgg gaatgcggca
    30181 attgtcacgc cgttcggcgc gaggctgggg agatccttcg tggaatgcac ggcgagatcg
    30241 acacggccgt caaggagctg aacctgaagc gccgaggtga agacgccgcg cccgccgatc
    30301 ttcatcaatg aggaatgctt gtcgatatca ccctcgggcc gaatgatctc caattcgaag
    30361 gcgtgttccc gcgacgcctt ctgcagcagt gcttgcacgg catgcgcctg ccacagggcg
    30421 agcgcacttc ctctggttcc aacacgaaaa cttttcggtt cactggatac ggacacgcaa
    30481 ttgcctgaca caaggaaaca cggggaggag ggaggcagcg ggggaaatgg atcccggctc
    30541 aataatcctt cctattctag agatgcccgg actaccggtt caagccattg tgcgacactg
    30601 ccggagttac tgccctcctg caacaccttg aagagcactt tctgttaggg aacggaggtt
    30661 caccgaaagc ggattcttga acgaaagcga ggggcaggac atcgcctgtc tcatcgggaa
    30721 ggcggagccg tgctcagcac actgtgttac aatacagtgt cttgggtgct tcgggcgccc
    30781 gcttttgcgt gcaacagtgc gataatgcat aaggtggttg atccggacgg cgcaatgttg
    30841 agcggatgat tgcgtgcttg agatgcgctg gaacgattgt atttcgcgca ctggtcagaa
    30901 ccaattgggc aggatatacg tacaatgacc gatgtagaaa aggtaacggc aaccctggca
    30961 gagtctactg aagattctgc cccggcgacc gctccctcca aaaagaagtc taaaaccgcg
    31021 ccggagctct ccccctatgc agtagtggag acgggtggca agcagtaccg cgtgtcagta
    31081 ggtgatcaca ttacgat
//
LOCUS       DASYWM010000177         5734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_363664, whole genome shotgun sequence.
ACCESSION   DASYWM010000177 DASYWM010000000
VERSION     DASYWM010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363664"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VGR29_07350"
     CDS             complement(<1..532)
                     /locus_tag="VGR29_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073444.1"
                     /translation="MRNPSVNTSAEPVSLPHATPDLKPVPSVNQKLSGPASERTSLLG
                     GPQVTSLPAFSVSTRLRGRHVLVDTDLTPDEINELLDTATRIKRLAKTGQPHGYLAGK
                     TLGLIFQHPSTRTRVSLEAGMAQLGGQAVFLGVNDLQMKRGESIGDTAQILSRYVDAL
                     VARVADHDDLIQLATSA"
     gene            complement(1145..2386)
                     /locus_tag="VGR29_07355"
     CDS             complement(1145..2386)
                     /locus_tag="VGR29_07355"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV2073445.1"
                     /translation="MSDSSTSKQNLGKVVLAYSGGLDTSVIIPWLKEHYECEVIAFAA
                     DVGQPDDLSGIEEKAIASGASRAYVADLKDEFIREYVFPTLRAGAIYERKYLLGTSMA
                     RPLIAKHQVEIAKLENAGAVAHGCTGKGNDQVRFELTFMSLAPELEIIAPWRQWEIRS
                     REDALDYAAEHDIPVTATAKKIYSLDGNLWHLSHEGGEIEDPWAEPSPAAYTMTVSPE
                     EAPEAPEYVTISFEEGYPVAIDGQRMDPVPLVEHLNELGGKHGVGRTVMLENRLVGMK
                     SRGVYETPGGTILVTAHNELEHLTLDKMTMRLKDQLATQYADLVYNGQWFTPAREALD
                     AFVGKTQEVVTGDVRLKLYKGSCEGVGFRSPYSLYQEDLATFGEDEVYEQADAEGFIR
                     LFGLGQKVANQRARSVQEVQV"
     gene            complement(2669..4348)
                     /locus_tag="VGR29_07360"
     CDS             complement(2669..4348)
                     /locus_tag="VGR29_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073446.1"
                     /translation="MATLPGGVPASLTRSSSILAILLMLIVLASGGLLRLVAALSLTP
                     HVDEASSLLAAHAVAERGLPILPSGTVYLQGATLSYLLAPFIWLGYGDLADLWLLRLV
                     IVVAGIAAVWFSYRLASTITGSVRTGVVMAMLVAFDPLSIQWSGHLRMYGLLQVLTIA
                     LALAWALVLARGATWPRLAAVVVLFWAAVFTHVGAALLGPAMFIAAVVVHRRALIHQG
                     RVGLALALSAVGAGTLMVLNRVLGSASVGDPQQKSSSKLLSFVGDNLLTPFANSPDEF
                     DWMALTRGGNLFWLIPGVLVAIATVAGGRLLLRKRSLIARTGAVVVLTMYWFPMAAVG
                     LFTSSPKERYVLHSHLLGYLFAAVIIVSVIRRWQDARAETPGIPPVFAQVFSVAIAVT
                     LISGATWRLQNPVVHPDHHAAMEYITEHHVAGEPIISSLPAIAYLAIDEEDRDDLYFL
                     AGEQDQSRARRYTRYTGEGVLIDYWVGADALVSSTRLQVFLRQHPDAWVVVDRERLQA
                     EWAYAGVVEDILRESTVPVAITAGGGLVLRPAQLSEADNARLWSLALDGNP"
     gene            complement(4573..>5734)
                     /locus_tag="VGR29_07365"
     CDS             complement(4573..>5734)
                     /locus_tag="VGR29_07365"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV2073447.1"
                     /translation="TIAAVPSRLASHTRNRIVQGAIVMLIALTFLLPNGLDIVRSLSQ
                     DDPPPGELVMWGNDPWMQNIIHESLRRDDPGGAGEFLQRQQDTQPPFRFIAYGGMYHP
                     DTVRRTYPDRRLEPAMVAILQNARAMRLNLETTQGYNPLQPLLYQQFIRALNGQQQDY
                     HYANLLHTGVDSPLLDLLNVRYIVVDRNIPRFRDDHRALAAGRPVVYQDQDVIIYENP
                     TVQPRAWMVYEVQPGHSQIGLARLANGDVDGAEVAFVQEDLPSVSPPEDGEEPQVTVA
                     DWRPDGMTLDVSHSGEGLLVVSQVYAEGWEATVDGEAVEVLQTDHALLGIPVGPGQHT
                     VEVRYDPDALTLGLWISGLTAVGSLGVVGYAGWTAFSHRRECQPGAQLSGQR"
BASE COUNT         1147 a         1819 c         1737 g         1031 t
ORIGIN      
        1 aggctgatgt ggcgagctgg atcaagtcgt cgtgatcggc gacccgggcg acaagcgcat
       61 cgacataccg gctaagaatc tgggccgtat cgccaatgct ctcgccgcgc ttcatctgca
      121 ggtcgttcac gccgagaaag accgcctgcc cgccaagctg ggccataccg gcctcgagcg
      181 acacacgtgt ccgcgtgctt ggatgctgga agatgaggcc aagcgtcttg cccgccagat
      241 agccgtgcgg ctggccagtc ttcgcgagcc gcttgatccg cgtggccgta tcgaggagct
      301 cgttgatctc atcgggagtg aggtcggtgt cgaccagaac atggcgaccc cgcaagcgcg
      361 tgctgacaga gaaagcagga agcgaggtga cctgaggacc gccgagcagc gatgtgcgct
      421 ccgatgcggg accggagagc ttctggttca cagacggcac cggcttcaga tccggtgtag
      481 catgcggcaa cgaaacgggt tccgcggacg tgttaacaga cggatttcgc atcgaaagct
      541 cccttcgatc ctggctcgca aacggttgcg gacgccagtc cacgccgcgg tgactccacc
      601 ggacggaacc gaaggggcgg gagacgtcga atcccgcggt accaccctcc ttgcgcaaca
      661 atcctgtcac cacctgagga cgcatccgct ctaatccagc ggatttgccc ctcaacggaa
      721 gtgaaggatt ccccggcgct agagcgccgg cctcaggaat gcagggttcc caccctgctt
      781 ccagttgcgt atctctcggc tgttgtatcc atgggagcca cccatccggt cctacttgcg
      841 agaggatgcc ctccacgctt tcttcggccg gccactcctg agcgcgttcg gcggggcgac
      901 gcagatcccg ggctttcacc atctccgggt cgctgatgcg cgcgtgccgc gtactggtct
      961 cgttcgtcgc ggggagtgtt ccaactccgg aacaccaaat ctcttcgctt gtaacacggt
     1021 actgggtctg gaaaagcgct aatggactgc gttggccact agcgcgtcgt acggtcgcga
     1081 cgcaccagcg gcctggcggg agagcgggga agcactccct gtccggacct catacagaat
     1141 ggtgtcatac ctgcacctcc tggacggacc gggcgcgctg gttggccacc ttctggccaa
     1201 gaccgaagag ccggataaag ccctctgcgt ccgcctgctc gtagacctcg tcctccccaa
     1261 aggtcgccaa atcttcctga tagaggctgt agggactcct gaaaccgacc ccctcacagc
     1321 tgcccttgta gagcttcagg cgaacgtcgc cggtcacgac ctcttgggtc ttcccgacga
     1381 aggcatcgag tgcttcccgc gcaggcgtga accactggcc gttgtagacg aggtcagcgt
     1441 actgcgtcgc aagttgatcc ttgagccgca tggtcatctt gtcgagggtc aggtgctcaa
     1501 gctcattgtg agctgtgacg aggatggttc cacctggtgt ctcatagacg ccccggctct
     1561 tcatgccgac aagccgattc tccagcatca ccgtccggcc aacgccgtgc ttgccaccga
     1621 gctcattcag gtgctcgacc agcggtaccg ggtccatccg ctggccatcg atcgctacag
     1681 gatacccttc ctcgaacgag atggtgacgt attccggtgc ctccggggct tcctccggag
     1741 agactgtcat ggtgtatgcc gccggtgacg gttctgccca ggggtcctcg atctcgccgc
     1801 cctcatggga gaggtgccac agattgccgt cgagcgaata gatcttcttc gctgtcgcgg
     1861 ttacaggaat gtcgtgctcc gcggcgtagt cgagcgcatc ctcgcgggac ctgatctccc
     1921 actgccgcca tggagcaatg atctccagct cgggcgcgag cgacatgaac gtcagctcaa
     1981 accggacctg gtcgttgccc ttgccggtgc agccgtgcgc gacggctcct gcattctcca
     2041 gctttgcgat ctcaacctga tgtttggcaa tcagggggcg cgccatggac gtgccaagca
     2101 gatacttccg ctcgtagatg gcgccggctc gcagcgttgg gaagacatac tcccggatga
     2161 actcgtcctt gagatcggct acatacgccc tggatgcacc ggaggcgatg gctttctcct
     2221 cgatgcccga gagatcgtcc ggctgcccaa catcggcggc aaaggcgatt acctcacact
     2281 catagtgctc tttcaaccaa ggaatgatga cggacgtgtc gagaccgcca gaataggcga
     2341 gcacgacctt gcccaggttc tgctttgaag ttgatgaatc ggacatgaag tcagttgctc
     2401 cggaagaggg aagagtcgaa acgcggattg ccgacgtaca ccccgtggcg gggtccgtta
     2461 taccgaacga gggtctcccg ttcggcaggt acgtgcgcgg ctgtcgtttc gacggagcat
     2521 cttcatgttt gctgatgact ttcttgagtg ccgttcgaat tccacaaaca gcaaatcggc
     2581 gtccaggaga caccccaccc cgcgcacgtg atgtttctca cgagcacggg attagcctac
     2641 cgggtgcggg cgcatcctgt caagtcgatc agggattccc atccagcgcc agcgaccaca
     2701 gccgggcatt gtcagcttct gagagctgtg ccggacggag gacgagccca cccccggcag
     2761 tgatggcaac tggtacggtg gactcgcgga ggatgtcctc cacaacaccc gcgtaggccc
     2821 actccgcctg caggcgctcg cggtcgacca cgacccatgc gtcagggtgt tgccgcagga
     2881 agacctgcaa tcgggtggag ctgaccagcg cgtccgcgcc gacccagtag tcgatcagca
     2941 cgccctctcc ggtgtaccgg gtatagcgcc gggcccggga ttgatcctgc tcaccagcga
     3001 ggaaatagag atcatcccga tcctcctcgt cgatagccag gtatgcgatt gctggcaggg
     3061 aagagattat cggctcaccc gcaacatgat gttccgtgat gtattccatg gcggcatgat
     3121 gatccgggtg caccacaggg ttctgcaagc gccaggtggc accggaaatc agggtcaccg
     3181 cgatggcgac cgagaagacc tgcgcgaaga cgggggggat ccccggtgtc tccgcccggg
     3241 cgtcctgcca gcggcggatc acactcacga taatgactgc cgcgaagaga tagcccagca
     3301 ggtgggagtg gaggacgtag cgctccttgg gggagctggt gaagagccca accgccgcca
     3361 tgggaaacca gtacatcgtc aggaccacaa ccgcccccgt ccgggcgatc aaggagcgct
     3421 tccgaagcag cagccggcct cccgcaaccg tcgctatggc gacgagcaca ccggggatta
     3481 gccagaacag gttgccgcca cgggtcagtg ccatccaatc gaattcatca ggggagttgg
     3541 caaacggcgt gagcagatta tcaccgacaa aggacaacag cttgctggag gatttctgtt
     3601 gcggatcacc cacgctcgct gacccaagca cccggttcaa caccatcaac gtgcccgcgc
     3661 ccacagcgga cagcgccagg gcaagcccta ctcttccttg atggatcaat gcacgccgat
     3721 gtacgacgac cgcggcaatg aacatggcgg gaccaagcaa tgccgcgccc acgtgggtga
     3781 acaccgcggc ccagaagagg acgaccacgg ctgccaggcg cggccatgtg gcgccccgcg
     3841 ccagcaccag cgcccatgcc agtgccagcg cgatagtaag aacctggagc aagccgtaca
     3901 tgcggaggtg cccgctccac tgaatgctca acgggtcgaa tgccaccagc atcgccatca
     3961 cgacgcctgt tcgcacgctc ccggtgatag tggatgccag ccggtaactg aaccagaccg
     4021 ccgcaatccc ggcaaccacg ataaccagcc gcaggagcca gagatcggcg aggtcaccgt
     4081 agcccagcca aatgaagggt gccagcaggt aggagagcgt tgcgccctgc aggtagacgg
     4141 tcccggacgg aagaatgggc agtccacgct cggccacggc gtgcgccgcc aacaggctgg
     4201 atgcctcatc cacgtgggga gtgagtgaga gtgcggcaac caggcgaaga agcccaccgg
     4261 atgcaagcac aatcagcatc aggagaatcg ccaggatact ggaagaacgg gtcaacgatg
     4321 cagggacgcc tccgggcagc gtagccatgc cggcagctgt tctttcggag gtcagatgtg
     4381 atgctgaccg gttgacgtac tctgacacga aacgttggac tccttgacaa tattgcttac
     4441 catgccaagg gtaccctata ccacgccgga agccatcgtc acatttccgg ctcaccaccg
     4501 gcgttcattc agaagcttgc aagtcgcagg ttccccggcg tgatccccac ctgcggcccc
     4561 cgggcggcgg cactaccttt gtccagacaa ctgcgctcct ggctgacact ccctccggtg
     4621 ggaaaaggcc gtccaaccgg cgtagccgac cacgccgagc gaaccgacag cggtgaggcc
     4681 gctgatccag agaccgagcg tgagcgcatc ggggtcgtag cgcacttcga ccgtgtgctg
     4741 gccgggacct accggaatcc ccagcaaggc gtgatccgtc tgcaacacct caaccgcctc
     4801 accatcgacc gtggcctccc agccctcggc gtagacctgg ctgacgacga gcagcccctc
     4861 gcccgaatgc gaaacgtcga gggtcattcc atccggtctc cagtctgcca ccgtcacctg
     4921 cggctcttcg ccgtcttctg gaggagagac gctcgggaga tcctcttgca cgaacgcgac
     4981 ctctgcgcca tccacgtctc catttgccaa ccgggcgagg cctatttgac tgtgtcccgg
     5041 ctgtacttcg tagaccatcc acgcgcgggg ttgcaccgtt gggttctcgt agatgatgac
     5101 gtcctggtcc tggtaaacca caggccgacc ggcagcgagt gcccgatggt catcgcggaa
     5161 ccgtgggatg ttccgatcca cgacgatata ccgcacattc agcaaatcca gcagcggcga
     5221 atcgaccccg gtatggagca ggttcgcata gtggtaatcc tgctgttgcc cgttgagcgc
     5281 ccggatgaac tgctggtaga gcagtggctg caacgggttg tatccctgcg tcgtttccag
     5341 gttgagacgc attgcccgcg cgttctggag aatcgcgacc attgccggtt ccagacggcg
     5401 gtccggatag gtgcgccgga ccgtgtccgg gtgatacatg ccaccataag cgataaaacg
     5461 gaaaggcggc tgggtatcct gctgccgctg aagaaactcc cccgctccac cgggatcatc
     5521 ccgccgaagc gattcatgaa tgatattctg catccagggg tcgtttcccc acatgaccaa
     5581 ctcacctgga gggggatcgt cctgcgagag ggatctcacg atatccagcc cgttgggcag
     5641 caggaacgtc agggcgatca gcatgacgat tgcaccctgc acgatacgat tgcgggtgtg
     5701 gcttgcgagc cgcgacggca ccgctgcaat agtc
//
LOCUS       DASYWM010000178         6700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_551668, whole genome shotgun sequence.
ACCESSION   DASYWM010000178 DASYWM010000000
VERSION     DASYWM010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551668"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VGR29_07370"
     CDS             <1..1458
                     /locus_tag="VGR29_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEV2073448.1"
                     /translation="VGDKFGAGELILPFVLQSAETMKKAVAQLETYLEKVEGSTKGTV
                     VLATVYGDVHDIGKNLVNTILTNNGYTVHDLGKQVPISRIIDKAVEVNATAIGLSALL
                     VSTSKQMPLAVKELHHQGIAIPVMIGGAAINPAYAHRALFVEPDTSYEPGVFYAKDAF
                     EGLSIMDRIVVPEERTALLETTLERARKTLNKPTRESFLNAADPNDTRRSETRILDEV
                     PTPPFWGVREMTDFMLDDIWPQMDMKTLFRLHWGGKGVKGEEWEALLENEFYPRLKRM
                     QESAKENGWLQPRVRYGFFPCNSENNDLIIYDPEDQDREIERMTYPRQPARERLCLAD
                     YFLPVESGKKDVVQFQVVTMGSVATEQTDRLQAAGDYSESYFTHGLSVSAAEGLAELV
                     HQRSREMLGIGEDGGKRYSWGYPSCPDLEHHLIVNRLLDMESIGVEVTEGYQFNPEQT
                     TAAIVVHHPDAKYFALLRTGGSPEAVQEPVAAAGD"
     gene            complement(1837..2487)
                     /locus_tag="VGR29_07375"
     CDS             complement(1837..2487)
                     /locus_tag="VGR29_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073449.1"
                     /translation="MDFQAPGATQLTRAEWDNNQFRRDMVYRREANVDSRVWDALVER
                     QARMIEDQSFAFTTVQTVDEGERVVRYMALENRLNRRILGKRWLEACRIKGPDMAANI
                     RTLPHSANDWTTYVFLNVSNFQNEPDGVFRGKRRGLLQEMMLASLVDIPDSRIIIGLA
                     ADLGGSPESYDHAFLDVIENEGEQLRRDARSAWAKKRRVFGPPVGNRHNEWSIPRP"
     gene            complement(2510..3229)
                     /locus_tag="VGR29_07380"
     CDS             complement(2510..3229)
                     /locus_tag="VGR29_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073450.1"
                     /translation="MSEVPTSESPIRINDGNGLTPTEELLTQLGERSFLRFWSHANPH
                     INRKEELCDLLVVCGDFVITFSDKNNEFKSTEDQWVGWRRWYRSAVTKSVNQLNGATR
                     NLLRDRVPVFKDKECTVPLGIPIPDPEHERHYRVAVASRAKELNDPEAFVPIISIDSG
                     VVGEALQDTAVPFVIGDVVPDRQFVHVMDVAGLRMVLTELDTVSDLAEYLDARESFIR
                     GKPANSADSEWEPTGAIHAVL"
     gene            3699..3965
                     /locus_tag="VGR29_07385"
     CDS             3699..3965
                     /locus_tag="VGR29_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEV2073451.1"
                     /translation="MTTRVINVRAKGQMTLPADIREELGLQDGGVIYLERRGKEFVLT
                     PADAIEDPTAGIFKEFAYTHNPDIEEEKVWIARHIAETADTYEK"
     gene            3962..4381
                     /locus_tag="VGR29_07390"
     CDS             3962..4381
                     /locus_tag="VGR29_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV2073452.1"
                     /translation="MTDRTPIAIDSNILLRHLLQDHSDHSPRASQLMLSVRKGQQRIF
                     CPSTVIFEAIHILHGRSRIPKPPIATVLKELIQLPSFLVAEEHAVVDALGFWAGQPAL
                     DYADCYHLALTKHLGMTEIYSFDKKMDRYPGVERIEP"
     gene            complement(4790..5011)
                     /locus_tag="VGR29_07395"
     CDS             complement(4790..5011)
                     /locus_tag="VGR29_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073453.1"
                     /translation="MASLLIDAISFQVIGELKGDPGHLLLIGDDGGWYDFDIVQVTIE
                     PIELGDSWEVDIASHSVIPFQSTVQNLIA"
     gene            complement(5135..5311)
                     /locus_tag="VGR29_07400"
     CDS             complement(5135..5311)
                     /locus_tag="VGR29_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073454.1"
                     /translation="MVGESRANDQWLLVVGTDGEYYGYHLRRNRLVRVKLDENWVRYK
                     ETGEATDERSRMKG"
     gene            complement(5882..6295)
                     /locus_tag="VGR29_07405"
     CDS             complement(5882..6295)
                     /locus_tag="VGR29_07405"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV2073455.1"
                     /translation="MPSRPVTVLERLQHAMNVHDLEALVVCFAPDFRSEQPNHPNRAF
                     LGNDQVRKNWATMFRSMPDLQVEVLRSAQQDDTIWSEWHWLGMLHDGSRQEMRGVLVY
                     GVQDDLIAWGRLYMEDTEAGGADIDASLQRLRRPS"
BASE COUNT         1461 a         1999 c         1830 g         1410 t
ORIGIN      
        1 gtcggcgaca agttcggcgc cggcgagctg atcctgccgt tcgtgctgca atccgccgag
       61 acaatgaaga aggccgtcgc ccagctcgag acctaccttg agaaggtcga ggggtcgacc
      121 aagggtacgg tcgtcctcgc cacggtctac ggcgatgtcc atgacattgg caagaacctc
      181 gtcaacacga tccttaccaa caacggctac accgtgcacg acctcggcaa gcaggtgccg
      241 atcagcagga tcatcgacaa ggccgtggaa gtgaacgcga ccgccatcgg cctctccgcc
      301 ctcctcgtct cgaccagcaa gcagatgccg ctggccgtga aggagctgca ccatcaggga
      361 atcgccatcc ccgtcatgat cggcggcgcg gcgatcaatc cggcctacgc gcaccgagcc
      421 ctcttcgtgg agccggacac gagctacgag ccgggcgtct tctacgcgaa ggatgcgttc
      481 gaagggctct cgatcatgga ccgcatcgtc gtgccggaag aacggaccgc actgctggag
      541 acgacgctcg agcgagcgcg caagacgctg aacaagccga cgcgtgaatc attcctgaat
      601 gccgccgatc cgaacgatac caggcggtcg gagacgcgca tcctcgacga ggtcccgacg
      661 ccgccgttct ggggtgtgcg cgaaatgacc gacttcatgc tggacgatat ctggccacag
      721 atggacatga agacgctgtt ccgcctgcac tggggcggca agggcgtgaa gggcgaggaa
      781 tgggaggctc tcctcgagaa cgagttctac ccgcgcctga agcgaatgca ggagagtgcg
      841 aaggagaacg gctggcttca gccgcgcgtt cgctacggct tctttccgtg caatagcgag
      901 aataacgacc tgatcatcta cgacccggag gaccaggacc gcgagatcga gcggatgacg
      961 tatccccggc agcccgcgcg tgagcgcctc tgcctggcgg actacttcct gccggttgag
     1021 tcaggaaaga aggatgtcgt tcagttccag gtcgtgacga tgggctctgt ggcgaccgag
     1081 cagaccgacc ggctgcaggc cgccggcgac tatagcgaga gctacttcac gcacggtctc
     1141 agcgtgtcgg ccgccgaggg cctggcggag cttgtccacc agcgctcccg ggaaatgctc
     1201 gggatcggcg aggacggcgg caagcggtac tcgtggggct atccgtcctg tccggacctg
     1261 gagcatcacc tcatcgtcaa ccggctgctg gacatggaat cgatcggcgt cgaggtgacc
     1321 gagggttacc agttcaaccc ggagcagacg actgccgcga tcgttgtcca ccacccggac
     1381 gcgaagtatt ttgcgctcct tcgcaccggt ggaagcccgg aggcggttca ggagccagtc
     1441 gctgccgcgg gcgattagat cgcgcgcctc cagagggagg gctgtacacc catgtacagc
     1501 cctccctctt tttgcgtcca agactgccac atacatctat ttggccgctg cccagcagtg
     1561 cccgcatcat ctttatatct atccagaaga cgtcgatgaa ctgaaggatc tcttcaagtt
     1621 cctcggcgag gagttgatgg tcatgatcta gcaagcttgg cagacggcca agctatttca
     1681 gtgaattgag accgagtaat ggagcctagg cagcattgcg acgatcggcc gttacccatg
     1741 cacggctggc ctcgtccatc acgtagggga tgtagtcaaa agtcagaaga cgcaaaaggc
     1801 ttgagaacct gaaagagtct gaaaagcaat cgaccactag ggccgcggga tgctccactc
     1861 attgtgacgg ttgcctacag gtggtccaaa cactcgccgc ttctttgccc acgctgagcg
     1921 agcatcccga cgcaactgtt ctccctcgtt ctcgataacg tcgaggaagg cgtggtcata
     1981 actctcgggt gacccgccca ggtcagcagc aagaccaata atgatgcggg aatcaggtat
     2041 atctaccagt gacgcaagca tcatttcctg aaggagccct ctcctcttgc ctctgaacac
     2101 cccatccggt tcgttctgaa agttgctgac attgagaaag acgtaggttg tccagtcgtt
     2161 cgccgagtga ggcaatgtcc tgatgttggc cgccatgtcc ggacctttaa tccggcaggc
     2221 ttccagccat cgcttgccta gaatccgtct attcagtcgg ttctccagcg ccatgtatcg
     2281 aacgacgcgc tctccctcat cgactgtctg aacggtggta aaggcgaaac tctgatcctc
     2341 gatcattctc gcttgtcttt caacaagggc atcccagaca cgactgtcga cattcgcctc
     2401 ccgtctatag accatgtcgc gtcggaattg gttgttgtcc cattcggcac gggttagttg
     2461 agtggcaccg ggggcttgga aatccattgg ttgaggttcg ccttcgtcgt cataggacag
     2521 catgaatcgc gccagtaggc tcccattcac tgtcagccga attcgctggc ttgcctcgga
     2581 tgaaagattc tctagcgtca aggtactcgg ccaaatcgga taccgtgtcg agttcggtca
     2641 aaaccattct caaccctgca acgtccatca catgcacgaa ctgccgatcc ggcactacat
     2701 cacctataac gaaaggtact gcggtgtcct gtagcgcctc acctacgaca cctgaatcta
     2761 tcgagatgat cgggacaaac gcctcagggt cattgagctc tttcgcccta cttgcaactg
     2821 ctacccgata gtgccgctca tgctcaggat cgggaatagg aatgccaaga ggaacggtgc
     2881 actccttgtc cttgaaaacc ggtactcgat cgcgcagcag gttcctcgtg gcgccattaa
     2941 gttgattcac ggacttggtc acagcggatc tgtaccatcg cctccagcct acccactgat
     3001 cttccgtcga cttgaactca ttatttttgt cagagaaagt gatcacgaag tctccacaaa
     3061 ctaccaggag gtcacagagc tcttctttac gatttatgtg tgggttggca tggctccaga
     3121 accgcaggaa ggagcgctca ccgagctgcg tcagcagttc ttcagtcggc gtgagtccat
     3181 tcccgtcgtt gatgcgaatg ggggattcac tggttggcac ttcggacacc ggttgttcct
     3241 ccgttgttag tttcccgttc acgccttgcc atgatctgat attgcgtcca tatctgcaga
     3301 gtaaagcagc caaagacgat cccaataaaa cgagtgacag cgacctccct cggctcaaac
     3361 aagcatacgg ccagagtcgg agcttttgac cacttcaagt ccaccgctct ctacgcccgg
     3421 ttcatgtggc acctgtttcg cacccgcacc agtaacttcg aggcccacta cttctcctac
     3481 ggtcaggata cgtctggcta ccacatcgag aagatcaaga ggctgatcct gccgaacccc
     3541 tactttcccc ctacgccacg ggctttgccg ccatcattcg gaagcaataa tccctttcca
     3601 atgcgggtgg tagcacttgg gacgaccacg tgtatgacac atcgtgataa cgtcgcacat
     3661 gatacgatgt cgtcatacaa ccaaaggggg ggtcgcacat gacaactcga gtaatcaacg
     3721 tccgggcaaa gggtcaaatg acactccccg ctgatatacg cgaggaactt ggcctgcagg
     3781 atgggggagt catctatctg gaacgtcgcg gcaaggaatt cgtgttgacg ccagcggatg
     3841 cgattgaaga tccgacggcg ggcatcttca aggagttcgc atacacgcat aacccggaca
     3901 tcgaggagga gaaggtgtgg attgcgaggc atatagctga aacagcagat acttacgaga
     3961 aatgaccgac cgcacaccaa tcgcaattga cagcaacatc ttgctacgtc atttgttgca
     4021 agatcacagc gatcattcac cacgggcatc gcaattgatg ctgtcagtac gcaagggtca
     4081 acagaggatt ttctgtccat ccactgtgat attcgaagcc attcacatcc ttcacggacg
     4141 gtcgcgaatc cccaagcctc ccatcgcgac ggtcctcaaa gagcttatcc agctgccttc
     4201 cttcctggtg gcggaagagc atgccgttgt cgatgctctc ggcttctggg ccggtcagcc
     4261 tgcccttgac tacgctgact gttaccatct tgccctcacc aaacacctcg gtatgacgga
     4321 gatctactcc ttcgacaaga agatggatcg ctatcccggc gtcgagcgga tcgagccata
     4381 gctcggtcag ggagagggat ccaatcaaac cgctgccgat gcctgaggac accgacggct
     4441 accagctcga ccctgagcag ccgacagcgc ctatcctcat tcaacacccc gacatgaagt
     4501 cttttgcgct gttccgcacc ggaggaagcc cggacgcggt tcaggagcca gccgccgccg
     4561 cgggcgacga gttttgtgtc ccaggctgcc aaatacatct atttggccgc tggccatgtg
     4621 gcagcctgtg ggcggtgcaa gatggccaca ctgccaagtt ctacattcac gaggcatgtg
     4681 gccggataca tcattggtcc taccagcgac actgcatgcg actctgtgac gggagccgtg
     4741 cgcaaccact gagcggatag ctcaccatct atgtgcatct accagatcat tacgcgatta
     4801 gattctgcac tgtggactga aacggaatca ctgaatggct tgcaatatcg acttcccagg
     4861 agtcgccgag ctcgatcggc tcgatggtga cctggacgat atcgaagtcg taccagccac
     4921 cgtcatctcc tatgagcagc aagtgaccgg gatcaccctt gagctcaccg atcacctgaa
     4981 acgaaatggc atcaatcagc aggcttgcca tggtgtaact ccttgcgaga tcctccctcc
     5041 aaaggattca tccgctctca accaagccac cagccatggc aatatcgaat ggtcgatggc
     5101 cggtctgcgg ccggtgtcgt gatactggtg ccccctaccc cttcatgcgg gagcgctcgt
     5161 cggttgcttc ccctgtctct ttgtagcgca cccagttttc gtcgagcttg acgcgaacga
     5221 ggcggttccg acgtaggtgg tagccgtaat actcgccgtc tgtgccaacc accagcagcc
     5281 actggtcgtt ggctcgcgac tcgcctacca cagtgaattc ttacccatga cttgccctgt
     5341 ttgtgatctt ccggcaccat cctgccggtg catcaataca aacgaggcat ccacgtgatc
     5401 cgtttcagta gatgtcgaaa ctccctgaac cgccgcttga tgcaatggct agagaaccct
     5461 cacccctggt cttatgtgga cgcgcccata aacggcttgg cggcatcacg ggtgctggct
     5521 ggcacatcac gacgatggat tcggaggctc agctcacgac tgccctcgag aagcaaatcc
     5581 aggacatcga gcccgggaga atctcaaccc ccgaagctgt ttgaaccaat gctgtatgag
     5641 gtggtccagc attccggcat tgttggcgac gcctggggct aagatggggc caaggggccg
     5701 ctgccgcttt tcgcctgccc agcggcctga ccctgcaacc ctgcgggggc agccctacgc
     5761 ccttgacacg catttccatc aagctagggg acgacattgc ctgcgacaat gtcgtcccct
     5821 actccccgca accagttttt tcgatgtcag gaaggatcct gtgtcacgga tggagctccg
     5881 gtcaggatgg ccgcctcaat cgttgcagcg aagcgtcaat gtcagctcca cccgcttccg
     5941 tatcctccat atagagccgg ccccatgcaa tgaggtcatc ctgaacccca tagacgagga
     6001 ccccccgcat ctcctgccgg gaaccgtcat ggagcattcc aagccagtgc cattccgacc
     6061 agatggtgtc atcttgttgg gccgaacgca aaacctctac ttgaagatct ggcattgacc
     6121 ggaacatggt tgcccagttc tttcgtacct ggtcgttacc tagaaacgcg cggttgggat
     6181 ggtttggctg ttcgctcctg aagtcgggag caaagcacac gacgagtgcc tcgagatcgt
     6241 ggacattcat tgcatgttgc agccgctcca gcacagtgac cggacgtgat ggcacgatta
     6301 tgctcctacg ttacgatccc ctatagcttc ctccgcaacc attgaatggc tttaccaatc
     6361 aacactccct tgataattcg ccccaaacat cccatgactc accccctgct aaaactcttg
     6421 ccaggatcag cttccgtgca agacctgtgc ctattagcgg ccggttgatt cttgctgccc
     6481 cggttattga gccacctacg gtgatttgca atgagattga tgtcccaagt cggccaggtg
     6541 ggggtgtagc ggacgacgta taggtgggca cgtcgtgact gagcgagttc ggctcgcagt
     6601 acctttgatg tggaacccga agagttcgat cagctgcaga agctttcacg ttccagggca
     6661 aggaactggt gctggttgaa acaagaagcg tggggggggg
//
LOCUS       DASYWM010000179        15398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_532874, whole genome shotgun sequence.
ACCESSION   DASYWM010000179 DASYWM010000000
VERSION     DASYWM010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15398
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532874"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..608
                     /locus_tag="VGR29_07410"
     CDS             219..608
                     /locus_tag="VGR29_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073456.1"
                     /translation="MNEPQTPQGGDMGEQDKIVVLTTDLFFGMRVRTTVKQLGYSVTI
                     TKDEAGTIDALAQQTPVLVLVDFNQPVDWPALAPLETAGVPVIAFGSHTDVEGFRAAK
                     AASVSRVVSNGEFSRTLPALIAKYRNS"
     gene            796..1824
                     /locus_tag="VGR29_07415"
     CDS             796..1824
                     /locus_tag="VGR29_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV2073457.1"
                     /translation="MSIASPAGSPLTIETAVEVFFGDLRLAPRSKKTYRQGIRKFVEH
                     METHEGIDPKTAPVTAISADHVTGFASILVPPDIRTPQEVSQMRTAQNNLAAVRKFCS
                     WLSAYDHHPTLSTDRLKVRIAAMMPRFTPPPPDVILSDLDRIVEHVLTGPREEKPHLE
                     LRRLKVRAMILFMFRTGVRVSELCALRRRDINLQDGTASIYRAKGGKSRTVHFDEQTG
                     EVLVAYWRARGDEIRGSGSLPAFSGRDKTGVPGNAISPRTVEHIVADLCTTLGIESEV
                     TPHSFRHGLATELVRRRVRESTVQTILGHASPQTTRIYVHKSALEVAEEYQDAFGRYR
                     SPGSHEGD"
     gene            1868..2044
                     /locus_tag="VGR29_07420"
     CDS             1868..2044
                     /locus_tag="VGR29_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073458.1"
                     /translation="MGVGTALAGCADGTSREAARGREEDSSNESVVKDLQATETWNLV
                     NATTPPGTQVPDGE"
     gene            complement(2118..3305)
                     /locus_tag="VGR29_07425"
     CDS             complement(2118..3305)
                     /locus_tag="VGR29_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073459.1"
                     /translation="MSRITKSVLVLVLSATMLGSGLTALAQPTPGPSIPVDVETRAQL
                     AAMVPGAEDLPVGYQFVGETFLAADEVASGTLDAGALTSAGFITQYVSVYENPESRSR
                     IRAYVSAWNDASAAEAGFALVEDEAMVAPNAALQDSEAGVGEDPRETTIGTYTLEDGT
                     SVGSVDVTFRRDNLLAGVAVETADGSAADAEMATELATRLDERIETVKNGESPAHTNL
                     ELPSQVISLTNQGQVLQAGFLGPAEVENIYGVQGSVLSGIDVSWVESVMVGDPATNAS
                     IIAVGTTTFGTPEDAAAAVEQSAELFTPLANQQAVEDVSLEGADAVQAYRYSSENAEG
                     DALDSYRLVFSTGTSLSVIDVQGAGSDIAAAETATQLALAQIACQTGSTCETPNVSAG
                     LGN"
     gene            complement(3408..3956)
                     /locus_tag="VGR29_07430"
     CDS             complement(3408..3956)
                     /locus_tag="VGR29_07430"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEV2073460.1"
                     /translation="MFFHSFMELGRILLVGSLAYLSLIVMLRVTGNRTLSKMNAFDFI
                     VTIALGSVLATVLLDNSVSLSEGAVAFFVLIGMQFIVTWLSVRFPIVDRLVKSVPVLL
                     VYEGRILSRAMKAARVTEDEVLTAVRNQGIPALDNVQAVVLETDGTFAVVGRTKEPTT
                     TLRNVDRSGIDIGHDSWQLKRK"
     gene            complement(4079..5296)
                     /locus_tag="VGR29_07435"
     CDS             complement(4079..5296)
                     /locus_tag="VGR29_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2073461.1"
                     /translation="MTSIDRQGHRLRNRRLTRRDFVASAAPLALGLTACGNEPDEQLE
                     EILDTPTPEPTQLPPTPTQPPIASPVAGYQDPARWAGRTLTIATAGVGEYLDALAKAF
                     FRAFETDTGAIVRHEQFGRDGIENLISQVEAGDVLWDVMLIPTNEVLQLSAKGYLAAI
                     DYNVVDPAALYPELTMQHGVGAQLYSTTMVYSVSLVEGPEDWSAFWDLTRFDGTRALR
                     RTPVGTLEFALLADGVAMADLYPLDVERAFLSLEKLREATLFYEDSKQPVELVRTGQV
                     GLASAWSVRATLPDVASLVNTEWNGGMISADSWVTPRGSSNPDIAMSFINFATRAVPT
                     ANFSRLQPFGPANKDALILLRPDVAASTPNHEDHFDKQFFENWSYWRDHREMLTAQFE
                     DWLLNPIATPEEA"
     gene            5631..5879
                     /locus_tag="VGR29_07440"
     CDS             5631..5879
                     /locus_tag="VGR29_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073462.1"
                     /translation="METYNIYMDELPTGEEFDGDEMVEVEFRVVPGSDDDGDPESNAV
                     IAGLDLVDLINLRDALQQEIDNYALTALEAQAGSLAEG"
     gene            6158..7795
                     /locus_tag="VGR29_07445"
     CDS             6158..7795
                     /locus_tag="VGR29_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV2073463.1"
                     /translation="MFTVNDLSKRFGESFIFEHVSFSINPGDRIGLVGPNGSGKTTLL
                     RILVGEESQETGSLSVAPGVTLGYLRQGFSDIPDGTLGDLVDVPLRGLIASHRALDEI
                     MGRLADPNADPASVARIFERANDLFESLGGYAALDELEALLDRFGLGEMAMDRPLDQL
                     SGGQKTRAGLAALLASRPDLLVLDEPTNHLDADAMAWLAEFLDSWRGAMLIVSHDRGF
                     LDDVVTKIFALDPLTGALSIYAGDYSDYVEARRHEEDEAEAAWSRQQAEVTRIKRDIR
                     AAESKARSIEASSIDYAVLKKAAKIARPAVVRKRKLERMLDAEDAASMPVRRWGMSLN
                     FAENNGGARDAIRLTDVHVSLGNQVILKGINLLVRHGDRVALLGPNGSGKTTLMRTIS
                     GELEPESGTVRIGSGVQVGYFAQEQQTLDPGKTVLEQAYAAAQMPESELRTFLHKFLF
                     DGETVHRRVAELSYGERARLMLATLVLKETSVLLLDEPLNHLDIDAREEFEQALAGFN
                     GTTVMVLHDRYAIDRLANRVVHMLDGGLVEITSETIA"
     gene            complement(8006..10222)
                     /gene="ppk1"
                     /locus_tag="VGR29_07450"
     CDS             complement(8006..10222)
                     /gene="ppk1"
                     /locus_tag="VGR29_07450"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEV2073464.1"
                     /translation="MSSTLQSGTTKKRPSRSEGKKGWKLPPLKPDLYINRELSWLEFN
                     RRVLEEMRKTSTPLLERVKFAAIFSSNLDEFFMIRVAGAKRKVVAGITDRGPDGRTPV
                     QAHRAIHDLAQELLTQQVVAVKGELFPALKDAGIEIVPYDELKRSERDALGHVFEREV
                     FPVLTPQAVDRGRRFPHVSNDSVNLIVVLKSKGESRFARVKVPALLPRFVPVPASSPE
                     GESGKVLVGGLNDVKHETMRFTLLEQVIAAHMDRLFPGSEVVASYAFHLLRDSDVEPD
                     EEDDDRQDLLEIMRETLSQRPFGTVVRIDIDESMPKPVRRWLMDQVHAEEDELYVVGG
                     PVGLDDLFELSRIDRPDLKDPSFVPATHFPWKDTDGRHEPQDVFDAVRENDILLHLPY
                     QSFSGVVDFVRAASQDADVVAIKQTLYRVGRDSPLIPALIEARDDDTQVAVLVELKAR
                     FDEENNISWAQTLETHGVHVAYGLSGLKTHCKITMVVRREADGLRRYCHIATGNYNAN
                     TARTYEDIGLLTCRENIAADVTNLFNVLTGYAEQVSYETLWVAPEFIREKLIDAIRIE
                     TESHRAHGNGHIILKMNSLVDRETIRALYAASREGVKIDLIIRGICCLRPGVPGWSET
                     IRIVSVVGRFLEHSRIFYFENIGHPKLYIGSADAMERNFDRRVEVIAPVESPELIEHL
                     RHKVLESYLKDTVNARCLQSDGSWVRVEPETGDEPFDVQRWFLDQYKSMSPVGSPE"
     gene            10395..11840
                     /gene="pyk"
                     /locus_tag="VGR29_07455"
     CDS             10395..11840
                     /gene="pyk"
                     /locus_tag="VGR29_07455"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV2073465.1"
                     /translation="MHPLTKIVATIGPATSSREQLEALIDGGVSVIRLNFSHGSQDDH
                     EAVYAAVREISHAKGRPVAILQDLQGPKLRIGDLVDGGPVVLERGGIFRLCTAQVQGT
                     AIRASVSYADLGRDVRPGDTILLDDGHLELLVEDVERDTPYGDEVITTVVHGGKLKPQ
                     KGMNLPGTVVSSPSMTEKDLEDLEFGVRLGVDMIALSFVREVEDVRSAQEKIRSLGGS
                     QPLIAKIEKPQAVKNLEAIVREVEGVMVARGDLGVEMRPEMVPLIQKRLIALANREGI
                     PVITATQMLESMIVSPRPTRAEASDVANAVFDGTDAVMLSGETAVGAYPLEAVRIMGR
                     IAKTVEQDSTWRASLRHEAPEVPVSRDAVAAAKAATSLAESIGAAAIAVLTETGRTAQ
                     RISQARPGIPIAAFTEHEFVANRLALWHGVVPVIDPLDDTIEGLVTRVDRRMQAMEIA
                     NAGETIVVIGAVPRSNGAQSVFLEIHHMDEQ"
     gene            complement(11928..13982)
                     /gene="ftsH"
                     /locus_tag="VGR29_07460"
     CDS             complement(11928..13982)
                     /gene="ftsH"
                     /locus_tag="VGR29_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV2073466.1"
                     /translation="MSQQDPKKPQFPLMRPDQDRNRQQRQRQGGDDPNGSQPPRRTPK
                     IPTWVVATVIIALVGWYIWEFFGPQDDPNVTEVPYSVVTEQIEADNISRVVLSETEIR
                     VELTEPVKYDSEREVVDPNAPTTQASIEQASEIEATLPQGVENTELLANLEATGATIV
                     GKTTGGSIWTSVLFSFLPFLLFLGLIIFMSRQMGRGQQNVFGFGRSKARQNDPERPQV
                     TFADVAGEDEAKQELMEVVDFLRNPGKYHALGARLPRGVLLVGPPGTGKTLTARAVAG
                     EAGVPFFSVSASEFVEMFVGVGASRVRDLFEKAKTASPAIIFVDELDAVGRQRFAGLG
                     GSNDEREQTLNQLLVEMDGFETNQEVIVMAATNRPDVLDPALLRPGRFDRQVTVGLPD
                     RKGREAILGIHTRGVPLSPRVDLEHIARGTTGFAGADLANLVNEAALNAARHNRKEIL
                     PDDFDEALDKILLGVARSGLIDPKEREVVAYHEAGHALAAHYTPGSDPLRKVSIVPRG
                     SAGGVTIQMPEEDRGLYSRTYLLGRLVGMLGGRAAEVVVFDEITTGAENDLKQATSLA
                     KRMVGLWGMSPEIGPIYLGTGEEHVFLGREITQDKSFSDATAQRVDAAVREIVENALR
                     EAIELNRRHRDRLEALVAALLEKETLDGPAVREIFGPGTPEDHEAGMHRRTPGAVELA
                     ND"
     gene            complement(14168..15025)
                     /locus_tag="VGR29_07465"
     CDS             complement(14168..15025)
                     /locus_tag="VGR29_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEV2073467.1"
                     /translation="MKPISLPRESQAKPVPEASATLPPDRAAARPSRRLVRCAPEIVD
                     ELPEPCLVRIDVNGCLAGTVLALPDAPRELAAGWSFMHGFYELTDHLDRVTLHGNRIS
                     VMVESGQDIDVRRLEAVGWAPPRPLPDHPVGEHFRIDEPALTKLIEATWDASRRDGAA
                     DGYVHAAVASAESVHCIARDLTSGLAAAKVLGWTILDASERDVSVLLIRGVVERRLIE
                     AAARIGISVVVTSGVPAADAFRAATRLALTIVGMAMSRTPALLVDGGHIDELPPAEDP
                     EEVQAGKQT"
     gene            complement(15114..>15398)
                     /locus_tag="VGR29_07470"
     CDS             complement(15114..>15398)
                     /locus_tag="VGR29_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073468.1"
                     /translation="MLPFVEGLLRRIDGEEFTTNDFIGLMLQVPETAAAYEAGMRAWG
                     ENRRQSKMVLHGQIIPGALRKSDMVQWNGFAHGEADEFSVPAWWRLKAPS"
BASE COUNT         3218 a         4579 c         4520 g         3081 t
ORIGIN      
        1 gtcctccggt agaaaatttc gatgacatgg gagcggtttc tcctggcaaa gaaggagcgc
       61 ccgcggacac gatccatagc aataaaaatt ctattgcgcc gtgagagaga ttgcaaatca
      121 tttcacgaac actgttcgga agctgggcgg atcgaaaggc ctcgctgtat gtcgcggtat
      181 gctgcgcaca aggttcgcag cgataggaag aggaagccat gaatgaacca caaacgccac
      241 aaggtggtga tatgggcgaa caggacaaaa ttgtcgttct caccacggac ctctttttcg
      301 gcatgcgggt gcgcacgacg gtaaagcaac ttggatacag cgtcactatc accaaggacg
      361 aagccggaac tattgacgct ctcgcccaac agacgccggt cctcgtcctt gttgatttca
      421 atcaacctgt ggattggccg gcactggctc cactcgagac cgccggcgtg ccggtgatcg
      481 ccttcggttc ccatactgat gtcgaggggt ttcgggcggc taaggcagca agtgtcagcc
      541 gggtagtttc caacggggag ttcagcagga ccctgccggc tctgatcgcg aagtaccgga
      601 actcatgact ccctggctcc agttcaccgg cagccgttgg aggaagtcgt gcgcatgaac
      661 ctgcatgtgg aaatgcctgc ctcgctcctg tcgcaatgtt gtcgcggaag cgatctccct
      721 tgatcgctcg caagaaccac tctatgcgta taattgtgta cgtacggtct tgtaccaaag
      781 cgacaggata gtcccatgag catagcttct ccagcaggat caccgctcac gattgaaacg
      841 gccgtcgagg tctttttcgg cgatctgcgg ttggcccctc gctccaagaa aacctaccgt
      901 cagggaatcc gcaagttcgt cgagcacatg gagacgcacg aggggattga cccgaaaacg
      961 gctccagtga cggcgatcag tgccgaccat gtaacaggtt tcgcctcgat cctcgtaccc
     1021 ccagacatcc ggacgccgca agaggtttcg cagatgcgta cggcgcagaa taatctggcc
     1081 gccgtccgga agttctgctc ctggctctcc gcatacgacc atcaccctac cctgtccact
     1141 gaccggctca aagtccggat agcggcgatg atgccgcgtt ttacgcctcc tccaccggat
     1201 gtcatactct ccgacctgga tcggatcgtc gagcatgtcc tgactggccc aagggaggag
     1261 aaacctcacc ttgagctccg gcgtctaaag gtacgggcga tgatcctgtt catgttccgg
     1321 accggtgttc gggtctctga gctctgcgcg ctccggcggc gtgatatcaa cttgcaggac
     1381 ggcaccgcca gcatctaccg ggcaaaaggc ggcaagagcc ggaccgtaca cttcgatgaa
     1441 caaaccggcg aagtactggt cgcttactgg agggcgcgag gcgacgagat tcggggttcg
     1501 ggatcgttgc cggcgttcag tggccgggac aaaaccggcg tcccaggcaa cgcgatcagt
     1561 ccccggaccg ttgagcacat cgtggctgat ctctgcacaa ctcttggtat cgagagcgaa
     1621 gtcacgcccc actcctttcg ccatggattg gccacggagc tggtcaggcg gagggtgcgg
     1681 gaatcgacgg tccagaccat tctcggccac gcgtcacctc agaccacgcg aatttacgtg
     1741 cataaatcgg cccttgaggt cgcggaagag tatcaggatg catttggaag atatcgatcg
     1801 ccggggtcgc acgagggcga ctaggaaggc acttacacgt cggcatttgc ttttttgggg
     1861 aagcgcattg ggtgtcggaa cagcactcgc tggttgtgcg gatggcacca gccgagaggc
     1921 ggcacggggg cgcgaagagg acagcagcaa cgagtcagtc gtgaaggact tgcaggcgac
     1981 cgagacctgg aacctggtca atgcaaccac tccacccggg acacaggtac ccgatggaga
     2041 gtaggaagag tgatatcaca ggacccgttg gcgtatgctg acgggtcctg tgatatcact
     2101 ctatccctgc agggctacta attgccgagc ccggcggaaa cattcggcgt ctcgcacgtt
     2161 gaacctgtct ggcaggcaat ttgtgccaac gcgagctgtg ttgcagtttc ggcagcagcg
     2221 atgtcggagc ctgcaccctg cacgtcaata acactcaagc tggtccccgt agaaaagacc
     2281 aggcgatagc tgtccaacgc atcaccctct gcattttcgc ttgaataccg gtatgcctga
     2341 acagcatcgg ccccctcgag tgaaacatcc tcgacggcct gctggtttgc cagaggggtg
     2401 aagagctcgg cggattgttc gaccgcggcg gcggcatctt cgggtgtacc gaaggtcgtc
     2461 gtcccaaccg cgatgatcga ggcattcgtg gcgggatcac ccaccataac cgattccacc
     2521 caggaaacgt cgatgccgga gagaaccgat ccctggactc cgtagatatt ctcgacctcg
     2581 gcaggaccaa gaaatccggc ctgaagaacc tggccttgat tggtcagcga gatcacctgc
     2641 gacggcaatt ccaggttggt atgggcggga gattcaccat tcttgactgt ctcgatccgc
     2701 tcatccagtc gtgtcgccag ctccgtcgcc atttctgcat cggcggcgct accgtcggcg
     2761 gtctccacag cgaccccagc cagcaggttg tcacgccgga acgtcacgtc caccgatccg
     2821 acggaggtgc cgtcctccag cgtgtaggta ccgatcgttg tctcacgcgg atcctcgccg
     2881 actcccgcct cgctatcttg caacgcggcg ttcggtgcga ccatggcctc atcctccacc
     2941 agcgcaaacc cggcttcggc ggcggaggca tcgttccatg cggagacata cgcgcggatg
     3001 cgcgatctgc tctcggggtt ttcgtacaca ctgacatact gggtgatgaa gcctgcactc
     3061 gtgagggcgc cggcgtcgag cgtgccggag gcaacctcgt cggcggctag gaaggtttct
     3121 cccacgaact ggtatccaac gggaagatct tctgcgcctg gcaccatcgc ggcaagctgc
     3181 gctcgagtct caacatcgac cgggatcgag gggccagggg tcggttgcgc gagggccgtc
     3241 agtccggagc cgagcatcgt cgcgctcagt acgaggacca ggacggattt ggtgattcga
     3301 ctcattccat acctctttcg ggcggtatta ctgcaacgca catctcaccg acctgcggtc
     3361 aacatggcgt aaagtgcttt tcctacttta ctttagcgct cgttggctca tttccgtttc
     3421 agttgccatg aatcatgtcc gatgtcgatg cccgaacggt caacgttacg caacgttgtc
     3481 gtcggctctt tggtgcggcc cacgacggcg aacgtgccat ccgtttcaag gacgacagcc
     3541 tgcacattgt cgagcgccgg aataccctga ttacggacgg ccgtcaggac ttcatcttcc
     3601 gtaacccgtg cggctttcat tgcccgggag agaatccgac cctcatagac gagcaacaca
     3661 ggcactgatt taactagccg gtcgactata ggaaatcgaa ctgacagcca cgttacgatg
     3721 aactgcatgc cgatcaggac aaaaaaggcg acagcacctt cggacagcga gaccgagttg
     3781 tcgagcagga cggtggcaag cacggaaccg agtgcaatcg taacgatgaa gtcaaaggcg
     3841 ttcatcttgg agagagtgcg gttcccggtt accctgagca ttacgataag cgaaaggtag
     3901 gcgagactgc cgacaagaag aatccgaccc agctccatga aactgtgaaa aaacacaaat
     3961 acctccgaag tgcagcaaaa aggaatcctc actccactcc aaccgcagtt gatcgcgtgt
     4021 ggttgggtcc tcgtcgcacg ccatgtgccg gaagaagtat ttctgcagga aaaggggctc
     4081 aggcttcttc tggtgttgcg atgggattaa ggagccaatc ctcgaactgg gccgtgagca
     4141 tctcacgatg gtctcgccag tagctccagt tctcaaagaa ctgcttgtcg aaatggtcct
     4201 cgtggttcgg tgtacttgcg gccacatccg ggcgaagcag tatcagtgca tccttgttcg
     4261 cgggtccaaa tggctgcagc cgcgaaaagt tggcggttgg cacggcacgc gtggcgaagt
     4321 tgataaaact catcgcgatg tctgggttgc tggacccgcg cggcgtcacc caggagtcgg
     4381 ccgatatcat ccccccgttc cactccgtat tgacgaggga cgccacgtcc ggcaaggttg
     4441 cgcgcacgct ccaggcactg gcaaggccga cctgtcctgt ccggaccagt tccactggct
     4501 gcttgctgtc ttcgtagaag agtgtcgcct ctctcagttt ctcgagggag aggaatgctc
     4561 tctcgacatc aagcggataa aggtcggcca tggcaacgcc atctgcgagc agtgcgaatt
     4621 ccagcgtccc cacaggtgtc cggcggagcg cgcgagtgcc gtcgaatcgg gtgagatccc
     4681 aaaatgctga ccagtcctct ggcccctcaa ctaacgatac ggagtaaacc atcgtggtgg
     4741 aatacagctg tgcgccaaca ccatgttgca ttgtcagctc tggatagagc gctgccgggt
     4801 cgacaacgtt gtagtcgatc gcggctagat acccctttgc ggagagttgg agtacctcgt
     4861 tggttggaat gagcatcacg tcccagagaa cgtcgccagc ctcgacctgc gagatgaggt
     4921 tttcgatgcc gtcgcgtcca aactgctcat ggcgcacgat ggcaccagtg tcagtctcaa
     4981 acgcgcggaa gaacgctttc gccaacgcat cgagatattc acctacccct gccgtggcga
     5041 tggtcagggt gcgccctgcc catcgggcag ggtcctgata accggcgacg ggtgatgcga
     5101 tcggtggctg cgtcggcgtg ggcggcagct gggtgggttc cggggtgggc gtgtccagga
     5161 tttcctcgag ttgttcatct ggctcgttac cacatgcggt caggccaagg gcaagcggcg
     5221 cggcgctcgc gacaaagtca cgccgtgtga gccggcgatt cctgagtcta tgtccttgtc
     5281 gatcaatcga tgtcatggtt tggacatact cgccgggttc tcagggcttc acatggcgca
     5341 tcaaggcgtg tcgggcagcg gcgtcacggg tctgcaaaga ataagcgaaa agggttgtaa
     5401 tgcgcttctg acgtgcagac cccgtcccgt cgtgcagaaa tcttcacttc cggatatcct
     5461 agaaggagcc ccggaagacg actgtgaggg accctggttc tttactccgc gacccgggca
     5521 atacgacatc atcgtggccg gggacattcg tcgggatgcc cgatgtgtca ggatgcgccg
     5581 ggagtctggt gcgtgtatcg gattgatcgc actcgcgata cggaggcggc gtggaaacat
     5641 acaacatcta tatggatgag ctcccaacgg gagaggagtt cgacggcgac gaaatggtcg
     5701 aagtcgaatt tcgcgtggtt ccgggttccg acgacgatgg cgatcccgag agtaatgccg
     5761 tcattgccgg tcttgatctg gtcgatctta ttaacctccg tgatgccctc cagcaagaaa
     5821 tcgacaacta tgccctgacc gcgctggaag cccaggctgg cagcctcgcg gagggataga
     5881 gcaacacgcg gccggttgcg aaaccgtgtc cggtgcgcta ttgtcgtgcg cggagcgagc
     5941 ccatcgggct tgctccgcat ttgcgtttcg attgattccc cacaatgcga gtggagggat
     6001 cccctcagga gcacaggacg ttgacggact agcactctcg tgagcaggca gacgcctcct
     6061 cacctttccc ttcccttcgg gccagtacca tggtcgaatg cagtcccttc aggggcatgc
     6121 tgaccggtac gcggcggagc gacggacagg agtgcatatg tttaccgtca acgatttatc
     6181 aaagcgcttc ggcgaatcct tcatctttga acacgtttct ttctccatta atccgggtga
     6241 ccgtatcggt ctcgtcggtc ccaacggcag cgggaagaca actctcctgc gcatcctcgt
     6301 cggagaggag tctcaggaga ccggctctct gtcggttgct ccgggggtga ctctcggtta
     6361 tctcaggcag ggattctcgg atattcctga cggtacgctg ggcgaccttg tcgatgttcc
     6421 cttgcgcggg ctcattgcca gccaccgggc gctcgacgag attatgggac ggttagccga
     6481 cccgaacgct gatcctgcat cggtcgcacg gatattcgag cgagccaatg acctcttcga
     6541 gtcgctcggg gggtatgccg ccctcgacga gctcgaagcg ctgctcgaca gattcggact
     6601 gggtgaaatg gctatggacc gcccgctcga ccagctctcg ggcggacaaa agacccgggc
     6661 cggactcgcg gccctcctcg cgtcccgtcc cgatctgctg gtcctcgacg aaccgaccaa
     6721 ccatcttgat gccgacgcca tggcatggct tgccgaattc cttgattcat ggcggggcgc
     6781 catgttgatc gtgtcgcacg accggggctt tctcgatgat gttgtgacga agatctttgc
     6841 cctcgatccc cttacagggg cgctttctat ctacgctggc gactacagcg actacgtcga
     6901 agccaggcgc catgaggagg acgaagcgga ggccgcgtgg agccggcagc aggctgaagt
     6961 gacgaggatc aagcgggata tcagggcagc ggagagcaag gctcgctcta tagaggcgtc
     7021 atcaatcgac tatgccgtgc tcaagaaggc tgcgaagatt gcccgtccgg ctgtggtaag
     7081 aaagaggaag ctcgagcgta tgcttgatgc ggaagacgct gcctcaatgc cggtacgccg
     7141 gtggggcatg tcgctcaact tcgccgagaa taacggtggg gcaagggacg ctatccgttt
     7201 gaccgatgtc cacgtctctc ttggtaacca ggtcatcctc aagggaatca atctgcttgt
     7261 tcggcatggc gaccgggtgg cattgttggg accgaacggg agtggcaaga cgaccctgat
     7321 gcgtacgata agcggcgagc tggaacccga gtccggaact gttcggattg gctccggcgt
     7381 gcaggtggga tattttgccc aggagcagca aacgcttgat ccggggaaaa cggttctgga
     7441 gcaagcctat gccgccgcac agatgccgga aagtgaactc agaacgttcc tgcacaagtt
     7501 cctcttcgat ggcgagactg tccatcggcg agtcgccgaa ctctcctatg gtgaacgggc
     7561 taggctgatg cttgccacac tcgtcttgaa ggagacaagc gtcctgctgc tggacgaacc
     7621 gttgaatcat ctggacatcg acgcacggga agaatttgag caggctttgg cagggtttaa
     7681 cggtacgacg gtcatggttt tgcatgaccg gtacgcgatc gatcggctcg caaatcgggt
     7741 cgtccacatg cttgacggcg ggctcgttga gataacatca gagactatcg catgatactg
     7801 aacgagcaag acaggggttg gggactctag cccagttatc ggcgaatggt cgatggttgg
     7861 tgtaggagtg cgacatatcc cggaatagac aaccacatta cattaggttg gtgaacttaa
     7921 gggatatgtc gcctttcgct ggaccttaag agagtagccg ccccctcaca cggcatagat
     7981 ctgtgctcca ggctgtcgga ttcccctact ccggcgagcc cactggagac atgctcttgt
     8041 actgatcgag gaaccagcgt tgaacgtcga agggctcgtc tcccgtttcc ggttccaccc
     8101 gtacccatga tccatctgat tgcagacagc gagcattcac cgtgtccttc aggtaggatt
     8161 cgaggacctt gtgtcgcaga tgctcgatca gttcaggtga ctccacgggc gcgatgacct
     8221 ccacccgccg atcaaaattg cgctccatcg cgtctgcgct gccgatatag agtttgggat
     8281 gcccaatgtt ctcgaagtag aagatccggc tatgctccag gaaccgcccc acgacactca
     8341 ctatgcgaat ggtctcactc caccccggga cgcctggccg gaggcagcag ataccacgga
     8401 tgataaggtc gattttcacc ccttcccggg atgccgcata gagtgcccgg atcgtctcac
     8461 gatctaccag ggagttcatc ttcaagatga tatggccgtt gccatgggcg cggtgggact
     8521 cggtctctat ccggatggca tcgatcagct tttctcgtat gaactcagga gcgacccaga
     8581 gcgtctcgta gctcacctgc tcagcgtagc cagtcagcac attgaaaaga ttcgtcacgt
     8641 ccgccgcaat gttctcgcga cacgtgagga ggcctatatc ctcataggtt ctggcagtat
     8701 tggcgttgta gtttccagtc gcgatgtggc aataccggcg aagtccatcc gcctcccggc
     8761 ggacaaccat ggtgatcttg cagtgtgtct tcagacccga taacccatac gcgacgtgaa
     8821 cgccgtgagt ttctagagtc tgggcccagg agatgttgtt ctcctcgtca aaccgcgcct
     8881 tgagctccac caataccgca acctgcgtgt cgtcgtcccg ggcttcaatc agcgcgggga
     8941 tgagtggcga atccctgcca acccggtaga gggtctgttt gatcgccacc acgtcggcat
     9001 cctgggatgc cgcccgcacg aaatcaacga caccgctgaa cgattggtag ggaaggtgca
     9061 ggaggatatc gttctccctg accgcatcga atacgtcctg cggctcatgc ctcccgtcgg
     9121 tgtctttcca cgggaaatga gtagccggta caaaggatgg gtccttcagg tctggtcggt
     9181 cgatcctgga tagctcgaag aggtcgtcca ggccaacagg gccgccaacg acatacagct
     9241 cgtcctcttc agcatggacc tggtccatca accatcggcg cactggcttt ggcatcgact
     9301 cgtcgatgtc gattcgcacg acggtaccaa agggacgctg cgaaagcgtt tcccgcatta
     9361 tttcaagcaa atcctgccgg tcatcatcct cctcatcagg ttcgacatcc gaatcgcgca
     9421 gcagatgaaa cgcataggaa gcgacaacct cgctaccagg gaagaggcgg tccatgtgcg
     9481 ccgcaatcac ctgctcgagg agggtgaatc tcatcgtctc gtgcttgacg tcattcagcc
     9541 caccaacgag caccttgccc gattccccct ctggcgaaga cgctggaaca ggaacaaaac
     9601 gtggcagcaa cgccggcacc ttgacccggg caaagcgcga ctcgcccttg ctcttgagga
     9661 ccacgatcag attgacactg tcgttcgata catgaggaaa acggcgccct cgatcaacag
     9721 cttgaggtgt caaaacggga aagacctcgc gctcaaaaac atggccaagg gcgtcccgct
     9781 ctgatcgctt gagctcgtca taggggacaa tctctatacc ggcatccttc agcgccggga
     9841 agagttctcc cttaacagcc acgacctgct gggtcagcaa ctcctgcgca agatcgtgga
     9901 tcgcccgatg tgcctgaact ggtgttcgac catcaggacc acgatcggtg atacccgcga
     9961 cgaccttccg tttcgcgccg gcgacacgaa tcatgaaaaa ttcgtcgaga ttggaggaga
    10021 aaatcgccgc gaatttcacg cgctccaaca atggcgtaga ggtcttgcgc atctcttcaa
    10081 gcacgcggcg gttgaactca agccagctca gctcccggtt aatatagaga tcaggcttca
    10141 aaggggggag cttccacccc tttttccctt cggaccggga gggacgcttc ttcgtcgtcc
    10201 cggattgcag tgtcgagctc attccgtacc gcatcccttt ccgcagccca ctcgtctcac
    10261 agctcactcc ggaccaccgc atgccggatc ctcatcaagt atcggcgata accccctatc
    10321 cgtcgcgcat tccgaagtga gaacatagtg ccctacgcag acggcgcaaa tacatggagg
    10381 atcggagatt cactatgcac ccacttacca agattgtggc caccattggg cccgctacat
    10441 ccagccgtga acaactcgaa gctctcattg atggcggcgt gagcgtgatc cgtctcaact
    10501 tttcccacgg ctcccaagat gaccatgaag cagtgtacgc agccgtccgg gagatctccc
    10561 acgccaaggg ccgaccggta gcgatccttc aggatcttca gggcccaaag ctccgtatcg
    10621 gggacctcgt cgatggagga ccagtcgtgc tcgagcgcgg cgggatattc cgcctctgca
    10681 ctgcccaggt ccagggcacg gcaatacgcg cgtcggtcag ctatgcggat ctcgggaggg
    10741 atgtacgacc gggagatacg atcctccttg acgacggaca cctggagctc ctcgtggagg
    10801 acgtggagag ggacacgccc tacggcgatg aggtgatcac gaccgtcgtt cacggcggta
    10861 agctgaaacc acagaaggga atgaaccttc ctggcaccgt tgtctcctcc ccttccatga
    10921 ccgagaagga tctggaagac ctcgagtttg gcgttcgcct cggtgtcgac atgatcgccc
    10981 tctcatttgt ccgcgaggtg gaggatgtac ggtcggcaca ggaaaagatc cggtcgcttg
    11041 gcggttccca acccttgatt gccaagatcg aaaaaccgca agcagtgaag aacctggagg
    11101 cgatcgtccg cgaggtagaa ggcgtgatgg tcgcacgtgg ggatcttgga gtcgagatgc
    11161 gtccggagat ggtgccgctg atacagaagc gcctgattgc attggccaac cgcgaaggca
    11221 tccctgtcat caccgccacg cagatgctgg agtcgatgat cgtctcgcct cgtccaaccc
    11281 gcgccgaagc gagtgatgtt gccaacgcag tcttcgatgg caccgatgca gtgatgcttt
    11341 ctggtgaaac ggcggtcggc gcttacccgc tcgaagcggt acgcatcatg gggcgcatag
    11401 cgaaaacggt ggaacaggat tcgacgtgga gagcaagctt gcgacatgag gcgccggagg
    11461 tgcccgtttc ccgcgatgcg gtggcggcgg cgaaggccgc gacgtcactg gcagagtcaa
    11521 tcggggcagc agccattgcc gtccttacgg agactggccg caccgcccaa cgcatttccc
    11581 aggcacgtcc cggcataccg attgcggcat tcaccgagca cgagttcgtt gccaatcgcc
    11641 tggcactctg gcacggtgtg gtaccggtta tcgaccccct cgacgacacg atcgaaggac
    11701 tggtgacaag ggttgaccga cggatgcagg ccatggagat cgccaacgct ggagaaacga
    11761 tcgtcgtgat cggcgccgtc ccccgctcca acggcgcgca atcggtgttc ctggaaatcc
    11821 atcacatgga cgaacaatag ccacaggaat aaagaccggc cgacagaaag agcgggttcc
    11881 tccttggaac ccgctctttc acctatgctc ttcggccggt actgcgtcta atcgttcgcc
    11941 agctcgacag cacccggagt gcggcgatgc attcccgcct catgatcctc aggcgttcca
    12001 ggaccgaaga tctccctgac cgccggacca tccagcgtct ccttctccag gagcgccgcg
    12061 acaagggcct cgaggcgatc acgatgtcgc cggttcagct caatggcttc gcgcagcgcg
    12121 ttctcgacga tttcccgcac agccgcgtca acccgctgtg ccgtggcgtc cgaaaacgac
    12181 ttgtcctggg taatctcccg accgaggaag acatgctcct caccggtgcc aagatagatc
    12241 ggcccaattt cagggctcat tccccacagg ccaaccattc gcttggcaag gctcgtcgcc
    12301 tgcttgagat cgttctccgc gcctgtcgtg atctcgtcaa agaccaccac ttcggcggcg
    12361 cgaccgccaa gcatacccac gaggcggccc aagagatagg tgcgactgta gagaccacgg
    12421 tcctcttctg gcatctggat cgtcacgccg ccggccgaac cacgcggcac gatactgacc
    12481 ttgcgcaggg gatcggagcc aggcgtgtaa tgagcggcaa gcgcatgccc cgcttcgtgg
    12541 tacgccacaa cctcacgctc cttcggatcg ataaggccac tccgcgccac accgagaagg
    12601 atcttgtcga gtgcctcgtc aaagtcgtcg ggcaggatct ccttgcgatt gtgacgggcg
    12661 gcgtttaggg ccgcctcgtt tacaagattc gcgagatcgg cacccgcgaa gccggtagtg
    12721 ccgcgagcga tatgctcaag gtcgactcgc gggctcagcg gcacaccacg agtgtggatg
    12781 ccaagaatgg cctcgcgccc cttgcggtcc ggcaatccga ccgtgacctg gcgatcgaag
    12841 cgaccgggcc gaagcaacgc cgggtcaagg acatcaggac ggtttgtcgc cgccatgacg
    12901 atgacttcct ggttggtctc aaacccgtcc atctcgacga gcagctgatt gagtgtttgc
    12961 tcacgctcgt cattggaccc gccaagaccc gcgaaccgtt ggcggccaac cgcgtccagc
    13021 tcatcgacga agatgatggc cggcgacgcc gtcttggcct tctcgaagag atcgcggaca
    13081 cggctcgcgc ccacgccgac aaacatttca acgaactcgg atgcggagac gctaaagaac
    13141 ggcacgccgg cctcacccgc gacggcccga gcggtcaacg tcttgccggt acctggaggg
    13201 cccacgagga gcacgccgcg cggcaaacgg gcgcccaggg cgtgatactt gcccggattt
    13261 cgaaggaagt ccacgacctc catcagttcc tgtttcgcct catcctcgcc agcgacgtcc
    13321 gcgaacgtca cctgcggccg ctccgggtca ttctggcgag ccttgctccg gccaaagccg
    13381 aagacattct gctgtccacg ccccatctgc cggctcatga agatgatgag gccgaggaag
    13441 aggaggaagg gcaggaagct aaagagaacg cttgtccaga tcgagccgcc agtcgtcttt
    13501 cctacaatcg tggcaccagt cgcctcaagg ttggcgagaa gctcggtatt ctccacgccc
    13561 tggggcagcg tcgcctcaat ctcgctggcc tgctcgatcg aggcctgggt ggtcggagca
    13621 tttggatcca cgacctccct ttcggagtcg tacttgacag gctccgttag ctcaacccga
    13681 atttcggttt cagaaagtac aaccctactg atgttgtcag cctcgatttg ctcggtgacg
    13741 acgctatacg gaacttcagt aacattcgga tcgtcctggg gtccgaagaa ttcccatatg
    13801 taccagccga cgagggcgat gatgacggtc gctactaccc acgtaggaat cttgggtgtg
    13861 cgacgaggtg gctgcgaccc attcgggtcg tctcctccct gccgctgtcg ctgctgacgg
    13921 ttccgatcct gatcgggacg catcagcggg aactgcggtt tcttggggtc ctgctgactc
    13981 atgcgttctg tctctcttga ctagggaatg gtgatcacag cgaatgacag ctcacatgaa
    14041 gtgtcactgc ggcacgcgtg tatggaacga tacactcctg cagcgtcaac tttagactaa
    14101 atcggcatgg gattacacgg aaaccggccg gtctgcacca ggactgcacg agttcggcga
    14161 cgaaacgtca cgtctgcttc ccggcttgga cctcttcagg atcttccgca ggtgggagct
    14221 catcaatgtg gccaccgtcc acgagtagcg ccggggttct cgacatggcc atacccacga
    14281 tcgtgagggc aagcctggtg gccgctcgaa acgcatccgc ggcgggaacg ccgctggtca
    14341 ccaccaccga gatgccgatc cgagctgctg cctcgatcag gcgccgctcc acaacgccac
    14401 gaatcagcaa cactgacaca tcgcgctccg aagcatccag tattgtccag ccaaggactt
    14461 tggcagcggc cagacccgaa gtcaggtctc tcgcgataca gtgaacggat tctgccgacg
    14521 ccacggctgc gtgcacatag ccgtcggcgg cgccatcccg ccgcgatgca tcccaggtcg
    14581 cttcgataag cttggtcagc gctggctcat cgatgcgaaa atgctcgccg accgggtggt
    14641 ccgggagcgg tcgcggcggc gcccagccga cagcttcaag tcgccggaca tcgatgtcct
    14701 gaccactctc aaccatgacg gagatcctgt tcccatgcaa ggtaacccgg tccagatggt
    14761 cggtcaactc gtaaaagccg tgcataaacg accatcccgc cgctaactca cgtggcgcat
    14821 caggaagagc gaggacggtt ccggcgaggc aaccattgac gtctatgcgg accagacatg
    14881 gctcaggcag ctcatcaaca atttccgggg cgcagcgtac cagccgacgc gaaggtctgg
    14941 cggcagcacg gtctggaggc aaagtggcag aggcttcagg taccggcttt gcttgggatt
    15001 cccgaggaag tgagattggt ttcatacagc gagcgctttc cgtcggtgcg agcggaccat
    15061 ggccgtgcac ctagtgtact cgttgtacgt acatcaaagt ctctctatac cggttaggag
    15121 ggtgctttga ggcgccacca ggccggaact gagaattcgt cggcctcacc gtgcgcaaag
    15181 ccgttccact gcaccatatc tgatttgcgg agagcacccg ggataatctg tccatgcagg
    15241 accatcttgg actgccgccg gttctcgccc cacgcgcgca ttcccgcctc atacgcagca
    15301 gcggtttccg gtacttgcag catgagtccg ataaagtcgt tggtcgtgaa ctcctcgccg
    15361 tcaatccgtc gaagaagtcc ttccacaaag ggaagcat
//
LOCUS       DASYWM010000180         4401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_402129, whole genome shotgun sequence.
ACCESSION   DASYWM010000180 DASYWM010000000
VERSION     DASYWM010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402129"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..1715
                     /locus_tag="VGR29_07475"
     CDS             411..1715
                     /locus_tag="VGR29_07475"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073469.1"
                     /translation="MFTKFFRNRQRSALWRNHDFLNLWSAETIAQFGSQLSLVAVPLI
                     AALTLDASPLEMGILAASGSAAGLILGFIAGVWVDRLPRKPIMIAMDVGRALTFAAIP
                     IAALMDILTFQLLLVVAILGGCQTVFFNAAWSAIIPNLVDRKHLSDANGKLFASLSLA
                     QVIGPALAGSLIGWFSGPMVMGITSGTFAWSGWFLTRIRHPEPPRNRSTPGSRPVLRE
                     IREGLDELLGSKLVRPLTTSAAVLNFGGFIFLAVYVLYLTDDLGLSTSGVGLVFAAGG
                     IGSLVGSIVAPTLARTFGVGRTVLWSATIFGVGNMLVPLAILVPAYALPLIVASETIA
                     WMSLIVFNINRFSLRQALTPDHLQGRVSSSTIAIISGVQMLGSLMGGVIGQVFSVHTA
                     LIVGTVGMLVAAWWIWDSPIPGIRDMPEHPEEVFVEAGTVAA"
     gene            complement(1825..2139)
                     /locus_tag="VGR29_07480"
     CDS             complement(1825..2139)
                     /locus_tag="VGR29_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073470.1"
                     /translation="MAWFEVGDAVRVTMPHAKNKRGLMGVHVMYTTLPEAKFDGAVGT
                     ITEVDPDGPQGAPQYLVDFRGHDNKAAIPWTVQWFREEWIRHAENPAVAARDAKRPIE
                     ST"
     gene            2462..2896
                     /locus_tag="VGR29_07485"
     CDS             2462..2896
                     /locus_tag="VGR29_07485"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HEV2073471.1"
                     /translation="MNYVARPDSNQHPRHVVRGECPVCNALLHVTRLECGSCGTAIEG
                     HFLLDRLSRLSPDQMAFLETFIKNRGVIKDIEVDLGISYPTVKARLDEVVRSLGYSTS
                     DSSLRPSQVREERRSILEALQNKRISAEDAARRLAALGERKG"
     gene            2896..3297
                     /locus_tag="VGR29_07490"
     CDS             2896..3297
                     /locus_tag="VGR29_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073472.1"
                     /translation="MTEPSRDPGGISAERLEVLRMVESKTITPDEGARLLESLDRSDR
                     AQASSSQTPVGPRNVRIQIRESGKRNADIDIVLPLGLVEVGLKMAQRFAPGRVPDSRI
                     IRESIESGYVGKLVDLHDGEDHIVIAVEPRS"
     gene            3375..>4401
                     /locus_tag="VGR29_07495"
     CDS             3375..>4401
                     /locus_tag="VGR29_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEV2073473.1"
                     /translation="MTNDSLMTRDDSGTTRQQVRVLPDQPLVLSVSNTSGCISITTSS
                     RTDVEIITERTDSKRGDDETHMELEVDGNRISVHPNWQIANTVGDLAKKVRNHLKEGF
                     NPDEWDFKKLKFSTHAAFDIQIDLPRELPDGSSVAVRTASGDVQAADISARVSVTTAN
                     GDVRLDRINGKLSAHSASGDVSLANITGSIEANTANGDIHVEGGEAWTALRAVNGDIR
                     VDGLTMKNARVTTVSGDVHADVTLNNRTSYTFDTVSGDITLNTTLPMTGASLNFRAVS
                     GDTKVSGAWTPATGKRTWQLAAGTEGPEIRVKAVSGDLRATATTDPTVTMVKEAVPQD
                     AQKEKEGG"
BASE COUNT          879 a         1363 c         1270 g          889 t
ORIGIN      
        1 agtcgcagcc aatcacgggc accccggttg acgtgccggt tcccagaaat tccagttcaa
       61 tcacaaaggc ttgctttcac caagtgcggc aagaagaacg atgagaggga ccgccgcgtt
      121 cctcttcgga tgagtatacg accgggtttc gcgcctgata cactggcccg aaagacgtta
      181 cgggcgcatc gatgtgcaca tttcgactcc cgatgtctca cgtagaatgc ttccgatgca
      241 gattgtcggc acaggacgtc ccggatgcga tgcaactcgt gccaccgtga tggcatgcta
      301 accgactgga ttccgttcta cggataaccg ccgctgcctc ctcctttcgg cgcatcaggg
      361 cagcgggatc aggcgcctcc tgacctcccg cctcttcggg agtcctttct gtgttcacga
      421 aattctttcg caaccgccag cgctctgcgc tgtggcggaa ccacgatttt ctcaacctct
      481 ggtcggcaga aaccattgcc cagttcggtt cccagctcag cctggttgcc gttccactca
      541 ttgctgcact gacgttagat gcaagcccct tggagatggg tattctcgcg gccagcggca
      601 gcgccgcagg tttaatcctc ggcttcatcg ccggtgtctg ggtcgatcgg ctgccccgca
      661 agccgatcat gatcgcaatg gacgtcggac gggcgctcac tttcgccgcg atcccgatcg
      721 ccgcgctcat ggatatcctc acatttcagc tgctgcttgt ggtggccatc cttggtggct
      781 gccaaacggt cttcttcaac gcggcatggt cggcgatcat tccgaacctg gtcgaccgca
      841 agcacctaag cgacgcgaac gggaaactct tcgccagcct gtcgctggcc caggtcatcg
      901 gccctgccct tgccggctcg ctgatcggtt ggttcagtgg cccaatggtg atgggcatca
      961 cctcaggcac atttgcctgg tcaggctggt tcctgacccg catcagacat cctgaaccgc
     1021 cgaggaatcg atcgactcct ggatcccgtc ccgtgctccg ggagatccgg gagggcctag
     1081 acgaactctt gggatcgaag ctcgtccggc cgctcacgac gagcgcggcg gttctgaatt
     1141 tcggtggatt catcttcctg gccgtctacg tcctctacct aacagacgac ctcggtcttt
     1201 ccacaagcgg tgtcgggctg gtctttgcgg caggcggtat cggatcactg gtcggcagca
     1261 tagtggcccc aaccctggcc cgaacctttg gtgttggccg tacagttctc tggagcgcga
     1321 cgatctttgg cgtgggtaac atgctcgtac cgctggcgat tctcgtcccg gcgtatgcgc
     1381 tgcccctgat tgtggcatca gaaactatcg cctggatgtc cctgatcgtc ttcaacatca
     1441 accgcttcag cctgcgccag gcgctgacgc cagatcatct ccagggacgt gtctcgtcat
     1501 cgacgatcgc gattatcagc ggggtgcaga tgctcggttc actgatgggc ggggtcatcg
     1561 gccaagtatt ctcggtgcac actgcactta tcgttggcac ggtcggcatg ttggtcgccg
     1621 catggtggat ctgggattca cccatccctg gcatacggga catgccggag catccggagg
     1681 aagtgttcgt tgaggccggg acggtcgcag cataagggca tctatcaacg acgcccgcag
     1741 tggacaagga tttttcgctc cctgaccggc tccaacgaac aggaaaagag cgcctcaaag
     1801 gaggcgccct ttcaagacga cgacttatgt cgattcgatc ggacgcttgg cgtcgcgcgc
     1861 ggctacggca gggttctccg catgccggat ccattcctca cgaaaccact gcaccgtcca
     1921 gggaattgcg gccttgttgt cgtgtccccg gaaatcgacg agatactgcg gtgccccttg
     1981 tgggccatcg gggtcaacct cggtgatcgt cccgaccgcg ccatcgaact ttgcttccgg
     2041 caaggtcgtg tacatgacat ggacgcccat taacccgcgc ttgttcttcg catgcggcat
     2101 agtcacccga acggcatcgc caacctcaaa ccaggccatg actcgacctt tccttccctg
     2161 aattccgctc cattgcaccc ggattcgctg tatcgggggc tgcccgtgtc ctgcccgagt
     2221 atagccgctt cggtatggaa acgatcccct gtccgtctat cgcgggcgat gggcatcccg
     2281 attctgcttc gcggcgtgca atcaaggtcg atattaattt tatcaatatg cctattgaca
     2341 ttattgattc ctgcctttag gatgcatgca tcgggctggt cgctgacccc cactggcccg
     2401 gtgaaggaca tggtctggcc cggtgcgcat cgggccggac cacacacaag ggaaagatgc
     2461 aatgaactac gtagcacgtc cggatagcaa tcagcaccct cggcatgtcg ttcgcggcga
     2521 atgccctgtc tgcaatgcct tgctccatgt cacccggctg gagtgtggat cctgtgggac
     2581 cgccatcgaa gggcatttcc tcctcgatcg gctgtcgcgg ctgtcgcctg accagatggc
     2641 cttcctggaa acctttatca agaaccgggg tgttatcaag gacatcgagg ttgacctcgg
     2701 tatctcctat cccacggtga aggcgcggct ggacgaggtc gtccgctcgc tcggctactc
     2761 caccagtgat tcgtcgctgc gaccaagtca ggtgcgggag gagcgccggt ccattctgga
     2821 agcgctccag aacaagcgca tctccgctga agacgccgcg agacgcctcg ccgcactcgg
     2881 cgaaaggaag ggttgatgac agaaccctca cgggatcctg gcggtatctc ggccgaacgt
     2941 ctggaggtcc tccgcatggt ggaatcgaag acgatcaccc ccgatgaagg tgcccgcctg
     3001 cttgagtcgc tcgaccggtc cgaccgtgcg caggccagct cgagccagac acctgtcggt
     3061 ccgcgaaatg tgcgcatcca gatcagggaa tcgggaaagc gtaatgctga catcgacatc
     3121 gttcttccgc tcggcctggt cgaggtgggc ctcaagatgg cccagcgctt cgcgcccggt
     3181 cgcgtaccgg actcacgaat cattcgcgaa tccatcgaat ccggatacgt cggcaaactc
     3241 gtcgacctcc acgacggcga agaccacatc gtgatcgcgg tcgagccacg cagctgatct
     3301 tccttcaaac gggtgttccg cgggaccgcc ctacgaatcg ggacaagatg ttcactagaa
     3361 agggtaacaa caccatgacc aatgattcgc tcatgaccag ggacgactcg ggaaccacac
     3421 ggcagcaggt gcgtgtcctc cccgaccagc cactcgtgct atccgtcagc aacaccagcg
     3481 gttgcatctc aatcacaacc agcagtcgca ccgatgtgga gatcattacc gaacgcacgg
     3541 attcaaagcg tggtgatgac gagacgcaca tggaacttga ggtggatggc aacaggatct
     3601 cggtccaccc caactggcag atcgccaata ccgtgggtga tctcgccaag aaggtcagga
     3661 accatctcaa ggagggtttc aatccggacg agtgggactt caagaagctc aagttcagca
     3721 cgcacgctgc ctttgacatc cagatcgact tgccacgcga gctccctgac ggatcctcgg
     3781 tcgcggtaag aacggcaagc ggggatgtcc aggccgccga tatctcggca cgagtctcgg
     3841 taacgactgc aaatggcgat gttcgtctcg accgcatcaa cggcaagctc tcggcccata
     3901 gcgcaagcgg cgatgtcagt cttgccaata tcaccggcag catcgaagcc aataccgcca
     3961 acggcgacat ccatgtcgag ggcggtgagg cctggactgc ccttcgcgcg gtcaatggcg
     4021 atatccgtgt ggatggcctg accatgaaga acgcccgggt gaccaccgtg agtggcgatg
     4081 tccatgcaga tgtcacgctc aacaaccgca ccagctacac gttcgacacc gtgagcggcg
     4141 atatcacgct gaacacgacc ctgccaatga ccggagccag cctgaacttt cgggcagtga
     4201 gtggtgacac gaaagtttct ggtgcatgga cgcctgccac gggcaagcga acctggcagc
     4261 tcgcggcagg caccgagggt cccgagatcc gcgtcaaggc ggtcagcggc gatctcaggg
     4321 cgacggccac cacggatccg acagtcacga tggtcaagga agcagttccc caggatgccc
     4381 aaaaagagaa ggagggtgga g
//
LOCUS       DASYWM010000181        18928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_570851, whole genome shotgun sequence.
ACCESSION   DASYWM010000181 DASYWM010000000
VERSION     DASYWM010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 18928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18928
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570851"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="VGR29_07500"
     CDS             complement(<1..1249)
                     /locus_tag="VGR29_07500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2073474.1"
                     /translation="MIVDRIFTPGLAQVAYLVADEAAGEVAVIDPCRDADVYISWADQ
                     RHLRITAILEMHVHADFVSGARELEIRTGAPIYASRLGDQEFPHRPLDDGDQVKVGQL
                     RLQAYFTPGHTPEHMAYLLYEGDGAASVALFSGDALFVGDVGRPDLLGKDATERLSHQ
                     LFETVTRRLATLPDELIVYPGHTAGSACGKKIGEAPHTSIGQEKMDNYAFQEKERDAF
                     VNTVMNDMPIPPAYYPVLKRVNTQDAVLIDELMDPRAFDSNKVEELIGKGALVVDTRD
                     KADFGQGHIPGSLFAGFGPNFHTWMGWVAPYDRDIVLVTGDNGSVDEAVTALRQIGLD
                     RVVGYLDGGMKAWTGETETLEQMTVGELWQQLDKGLAVLDVRSDAEWRDDHIGGARHL
                     FAGEIAQGTVPPDSLGPDRRTAVI"
     gene            1780..1932
                     /locus_tag="VGR29_07505"
     CDS             1780..1932
                     /locus_tag="VGR29_07505"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEV2073475.1"
                     /translation="MAQILWIILVVIVAIWLIGLLADIAGSLIHLLLVVALIILIYNL
                     VVGRRA"
     gene            complement(2019..2258)
                     /locus_tag="VGR29_07510"
     CDS             complement(2019..2258)
                     /locus_tag="VGR29_07510"
                     /inference="COORDINATES: protein motif:HMM:NF022716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEV2073476.1"
                     /translation="MSDNNNEQDQNLQQNPVKDPEDWTTGDEPMTGPQASYLRTLLQQ
                     AGKSDADLDESMTKARASEMIDELQKETGRGNQQQ"
     gene            2768..2959
                     /locus_tag="VGR29_07515"
                     /pseudo
     CDS             2768..2959
                     /locus_tag="VGR29_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428384.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
     gene            3050..3247
                     /locus_tag="VGR29_07520"
                     /pseudo
     CDS             3050..3247
                     /locus_tag="VGR29_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
     gene            3567..4598
                     /locus_tag="VGR29_07525"
     CDS             3567..4598
                     /locus_tag="VGR29_07525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2073477.1"
                     /translation="MPRPLSVLDLTRVMEGESSSEAIGTTVAMAHLAEELGYTRIWFA
                     EHHNAPGLASGAPEILISHVASRTSHIRVGSGGVMLPNHAPLKIAEVFRLLEALHPGR
                     IDLGLGRAPGTDQKTAYALRRNPEAMSADDYPQNLAELLAYEDRSFPPNHLFATIQAI
                     PVDVPLPPIWLLGSSAFSGILAAQTGLGFSFASHINRPAAVQVMRAYREQFQPSVHFE
                     APNAILAASVVIGETMEQAEDLSTIVKVGIYRLVSGQMGPAPSLEDARKVEIPPHMQI
                     QMGSMLGNQFVGTPDSVARELKEFADQAGADELMLTTWIPMRENREYVLRELKRAWDS
                     VDDRGVTAN"
     gene            5130..6005
                     /locus_tag="VGR29_07530"
     CDS             5130..6005
                     /locus_tag="VGR29_07530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV2073478.1"
                     /translation="MLPSTADTFDASGGMHAAHGDPGATRSTGSIVGQYSLSQILGIW
                     AAAVVPMVLLAWVATPLVGDRLNLGVGDANREAFTRAGFITLGLVWQFVLVMIIVKRE
                     EGDLGWSTIRRRCWLNTPHDPHTGETRRKLWWWLAPFIVGVLAIQLIPLQAVWEWMFP
                     FLAEPDKYSFAELLQSEERKEALEGAWQVLGLFLVLGLFNTVIGEEFLFRGILLPKMH
                     GVFGKHDWIANGLLFGLYHLHQPWSILSSMVRGVLLYALPSRRFRSAWFGIIVHSMQT
                     VFFIFVALGLVLGLA"
     gene            complement(6186..7376)
                     /locus_tag="VGR29_07535"
     CDS             complement(6186..7376)
                     /locus_tag="VGR29_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEV2073479.1"
                     /translation="MTQWPRLNLASGPVDVTTETLRAMQQPVVYHYDPVFIETFARTS
                     ELLKQVFRTSNDVIILQAEAILGLEAAAASVIEPGTRVLNLVSGVFGKWYEDFIRRYG
                     GEPIELAVPYNAAIDPEDVRRLLQTQPDISVLSVVHSETPSGTINPVGEICRIAKEFG
                     VVTIVDTVSGLGSELFSPEEWGMDIAVAGPQKCLGGVPGLSLVAVSADAWAAMERKKE
                     PLRGSFLSMLDWKTTWLEQRRFPYTPSVSDIYALESVLGQALREGMETVVDRHQTSAK
                     AARAAVNALGLDLWPESPEIATSCCTAVRIPEGITDDALRETMRQRYGVMISGGYGEL
                     SGKLFRLGHMGMSAHPTTLVAQVGMLERALDDLGIKVNLGAGVGAALGAFKGWNDAEG
                     RFTV"
     gene            7527..8306
                     /locus_tag="VGR29_07540"
     CDS             7527..8306
                     /locus_tag="VGR29_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV2073480.1"
                     /translation="MPRIAANLTMLFSESPFLERFDRAAAAGFTAVEFQLPYEHDPND
                     IADRLSRNNLELVLFNLPAGDWGAGDRGVAAIYSRLAEFQEGVGKALEYAAVLHPPLL
                     NCLAGIAAPTPENDLALLQNVRFAAEQLEEHGIRLTLEPVNTHDVESFALPTSRSVIE
                     LIAELEHPNVGLQLDVYHSIRMGEDPFALITDLGNEIAHIQVADVPGRHQLGTGEVDF
                     RKLFDTIDASGYEGWVALEYIPEGPTEEGFGLLREIGLLPQ"
     gene            8357..9025
                     /gene="upp"
                     /locus_tag="VGR29_07545"
     CDS             8357..9025
                     /gene="upp"
                     /locus_tag="VGR29_07545"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV2073481.1"
                     /translation="MDAAQRLAEFPTLHVSNHPLIRHKQTLLSDERTDTRMFRELVRE
                     LTSLLVYEAMADVPLTTFRYRTPLEETTGFELEPRIGLVPIIRAGLGMVEAAADIIPH
                     SEVWHLGMYRDEQTHEPVSYYNKLPKTCPDDLIVVLDPMLATGGSARDAIRVLKAWGA
                     KWIKFVGIIAAPEGVHELQQSHPDVDVYVSVLDRELDENRFILPGLGDAGDRLFGTVR
                     QEQE"
     gene            9022..10020
                     /locus_tag="VGR29_07550"
     CDS             9022..10020
                     /locus_tag="VGR29_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV2073482.1"
                     /translation="MIHSLPLIVIITTGGTIGSRLDLTTGAVSAAETAEDLIALVPDL
                     PAIARIELKPWISVNSWNISPAMMFDLLQEVRATLDRPEVAGVVITHGTDTVEETGVM
                     AELLVESEKPVVFVAAMRNLSELGSEGPRNLLDAVSVAADPDSIGRGALLVANETIHA
                     ARYLVKTNTTNPASFLSPDYGPMGLIPATGLRYLHAAPKRRILNVPMLGGPVHVVKAV
                     SGSDSSLLDWLIDQGTKGIVLEGSGAGNVPAGMLPGVERAIDADIPVVLTTRVLWGFL
                     AATYGSGGARGGGFDLQQMGVIPAPHLPSPKARIVLMLALGAILSLDEIRDLLADP"
     gene            complement(10048..10311)
                     /locus_tag="VGR29_07555"
     CDS             complement(10048..10311)
                     /locus_tag="VGR29_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073483.1"
                     /translation="MPLLKRLAEQAMSDQAFRAEAGADLDGALERHGYDLNERELSLV
                     GRFRAALEEAGVDLFLKDEIDLDQIFAGDDGGHIERLLDSRGG"
     gene            complement(10450..11091)
                     /locus_tag="VGR29_07560"
     CDS             complement(10450..11091)
                     /locus_tag="VGR29_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV2073484.1"
                     /translation="MKRVLDQLSHPGVVAIVRLDDYSAAVEMVRALYEGGIRAVEFTY
                     TNPRAGNAISSVVDVMGDAVAVGAGTVLDPETARAAMLQGASFVVTPTTNIETIRMCQ
                     RYDVPTVIGAFTPTEMLTAWEGGATFVKLFPASAVGPTYIKDVHGPLPQIPIIPTGGV
                     SLENCAEFIKAGAAGVAIGSNLVHAKTVTDKDWSLLTERARALVDAVTQARSI"
     gene            complement(11210..13072)
                     /gene="pepF"
                     /locus_tag="VGR29_07565"
     CDS             complement(11210..13072)
                     /gene="pepF"
                     /locus_tag="VGR29_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV2073485.1"
                     /translation="MIDQTATDVLTRADVAIEDTWDLSQMFTSDEAWDAAAEEAPDKV
                     ARVASYRGRFGEGPTVIREALDALYEAQQRLQVLMVYAALRRDEDQTNPEAMARYERA
                     VALSIKAGEELAFVQPELVSLPGDALQVAIESPELVGYRHLLEDLERQRAHVLSAEIE
                     EILAQMADVTRAPSEAFSALDNADIEYGTVVDEDGNEVTLTKGRYGQLMESRDREVRR
                     KAAEKFAKPYLDHTYTLATLHGSSVRKDVVGARLRGYESAQQQALFDDNVPVTVYSNL
                     INTVRDSALPIVARYMELRRRALGVDELKRYDLRVPLAPESRKQYGYRDAVEIVLKGV
                     GALGEQYVSDLRQGFESRWVDVHETKGKRSGAYSWGAYGAPPVMLMNWNSTLSDVFTL
                     AHEAGHAMHSFYAEKAQPIQYAGYSIFLAEIASTVNEVLLSWHLLAQDEGRDPVQRFS
                     LLNRFADTYVGTVIRQTMFAEFEQMTHAVVEAGNALTPDVLSAFYSDLSGSYTPGVDI
                     DEGIRIEWGRIPHFYNAFYVYQYATGLSSAINIATAVRDEGDPARERYLAMLSAGSSD
                     YAMNVLNTAGVDLETPAPIQSGVAEFDRVVSELESLAEEGVLEKAAAALEAEQA"
     gene            13313..14104
                     /locus_tag="VGR29_07570"
     CDS             13313..14104
                     /locus_tag="VGR29_07570"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2073486.1"
                     /translation="MRIYFELALRAFRQLFAYQAAMLAGIFTNSVFGVMLSAVYLALF
                     RSHADGTSVEGFSARETVTYVWIGQALIAPVMIWGWWEIIKTIRTGAIVTDMLKPLSF
                     FGFWLSRDLGRAMGHLLLRGVPTLIIGALFYDLVYPGTPGRWLAFLVSIPFAVIVSFC
                     FRFIINLWGFWVLDHRGIAGIAIVIVGVASGHLLPIAWYPNAFRDVLNMLPFRAIVML
                     PVEIWLGQVGIVEGLGLQVFWILVMTAAAHWLQSVAERKVVVQGG"
     gene            14109..14918
                     /locus_tag="VGR29_07575"
     CDS             14109..14918
                     /locus_tag="VGR29_07575"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2073487.1"
                     /translation="MTLRQECRIAIRIAGIRLRGQMAYPASFWMQIFSSFVIHAAEIL
                     ALLAMFNRFNDMGGWTLGEVAFLHGISMTAFSIGDTIAVGLDSVPNQIREGEFDRVLI
                     RPLSSWIQTVVSEVSLRHLGQCAQGLVILGFALTWTDIQWTLQKALFMPVVIATGVAL
                     FVALFTLVAILSFWTVNSIEVVNAFTYGGSDLAQFPLHIFDRWMRNLFLFVIPVGFTA
                     YYPALYILGKDDPLGMPDTFRFLGPVVTAGFCLVVAWGWNQGIRHYRSTGS"
     gene            15028..16038
                     /locus_tag="VGR29_07580"
     CDS             15028..16038
                     /locus_tag="VGR29_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2073488.1"
                     /translation="MDGPIIEARNLEKRFTIRRHREGAFGAMRSLVDRGGNAVSAVKD
                     VSFSINRGEMVGYIGPNGAGKSTTIKMLTGILVPSSGEATVAGFTPWESRKQLAQQIG
                     VVFGQRTQLWWDLALTDSLELLRHMYRIPEKRFRENLDRARELLVLDDFMHTPVRQLS
                     LGQRMRGDLAAALLHDPKILYLDEPTIGLDIVAKARIREFLAALNRETHVTVLLTTHD
                     LADIEQLCRRIIVIDRGQVVYDGELGTLRNRFGGKRHVILDFDQDPGEAISTLAGNNV
                     EIVHHNGPRVRVAFDRETTSAPRVLALASHHGTVRDMSIEEPAIEDVIRQMYEGPDSH
                     HP"
     gene            16400..16768
                     /locus_tag="VGR29_07585"
     CDS             16400..16768
                     /locus_tag="VGR29_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HEV2073489.1"
                     /translation="MTASTQIICRDCNNAFIYTDAERDFRESRGLTRPELCPECRGKE
                     RARRNGDLISLYQRVDALDPFLISGETPRNGRNHRIRDTRQQYTTTCAACGSETRVPF
                     VPRGDRPVYCRSCYNARKGR"
     gene            complement(16881..18278)
                     /locus_tag="VGR29_07590"
     CDS             complement(16881..18278)
                     /locus_tag="VGR29_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV2073490.1"
                     /translation="MTGQPAEALMATQTQKHVTDKAKDHLWPHQLPWVDVAENEGLKV
                     FDRGEGSTLWDIEGREYLDGIAGLWVVNAGHGRGEIAEAMAKQAAKLSYVSAVQYTTE
                     PAAHLAHKLAEITPGDLSRSYFCSGGSEAVETAMKIAKQVQAMRGFPRRYKIIARRGS
                     YHGMTHGAMSLTAAREEKFFGPFMYGVSYAPPPDQYRSPFELEGEASDIMAAKFVEQE
                     IISQGPETVAAVIGEPISSAAGVHVPSPKYWQMLREICDRHGVLLIADEVINGFGRTG
                     TMFATEQFGFVPDIMTIAKGLTSGYMPMGAAIAGPKVFEVFESQPDVPMGHLLTFGGH
                     PVAAAAALRNLEIFEDEDLVSRGAEMGIYLRERLEELRNHPSVGDVRGVGLLQAIEVV
                     KNKEAKTPFSKNDKFCKVLGQKVLEKGLITRTWNIVHFAPPLVVTRDEIDRMVSIVDE
                     SLTEVEKEFASDIEG"
     gene            18464..>18928
                     /locus_tag="VGR29_07595"
     CDS             18464..>18928
                     /locus_tag="VGR29_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073491.1"
                     /translation="MTASPSSRLVIDYRNATVDAVGPMHGISDAELNELQPYVSEQHD
                     RIVDEHRNSVQRWQDLPDNAALADDVVQFANDARRTYRDFILIGIGGSSLGAIATIQA
                     LCHPYRNLLPDEERGGPRFFVLDNPDPEKVRATLETVDLSRTLVNVVSKSGQT"
BASE COUNT         3638 a         6001 c         5381 g         3908 t
ORIGIN      
        1 agatgacagc ggtcctgcga tccggtccga gagagtccgg aggcaccgtg ccctgcgcga
       61 tctcgccagc gaagagatgc cgcgcccctc cgatatgatc gtccctccac tcggcatcag
      121 agcggacatc gagcaccgcc aatcccttat caagctgctg ccaaagctcc ccgacggtca
      181 tttgctccag cgtctccgtc tcgccggtcc aggccttcat accgccgtcg aggtagccga
      241 cgacgcggtc aagcccaatc tgccgcaagg ccgtaacggc ttcatccacg cttccgttat
      301 caccggtgac gagcacgata tcgcggtcgt agggtgctac ccaacccatc catgtatgga
      361 agttgggacc aaaccccgcg aagagtgatc cggggatatg cccctgaccg aaatcggcct
      421 tgtctcgcgt gtcgaccacc agtgcgccct tgccgatcag ctcctcgacc ttgttggagt
      481 caaatgctcg cggatccata agctcgtcga tcagcacggc gtcctgcgtg ttgacccgct
      541 tcaggacggg gtaatacgcg ggcgggattg gcatatcgtt catcactgta ttgacgaagg
      601 catcccgctc cttttcctgg aacgcatagt tgtccatctt ctcctgcccg atggacgtat
      661 gcggcgcttc gccgatcttc ttgccacagg cggagcccgc ggtgtgcccg gggtagacaa
      721 tgagttcgtc gggcaatgtc gcgaggcgcc gcgttaccgt ttcaaacagc tgatgactaa
      781 gccgctccgt cgcgtccttt cccagcagat ccggccgccc cacgtccccc acaaagagcg
      841 catcaccgga gaagagagcc accgacgctg ctccatcccc ttcatacagc aggtatgcca
      901 tgtgctccgg tgtatggccg ggcgtgaagt aagcctgtag ccgaagttgt ccaaccttga
      961 cctggtctcc gtcatccagt ggccgatgcg ggaattcctg gtcgcctaac cggctggcat
     1021 agattggtgc gcctgtacgg atttccaact cccgcgcccc tgagacgaaa tcggcatgca
     1081 cgtgcatctc caggattgcg gtaatccgca gatgtcgctg atcggcccac gaaatataga
     1141 cgtcggcatc gcgacatgga tcgatgaccg ccacttcacc tgcagcctca tcggctacaa
     1201 gataggcaac ctgggcgaga ccgggggtaa aaatgcgatc gacgatcatc acagctcctg
     1261 tggaacatat ttggctgcgg ccctattgac gacacgaatg gagcatttca ccatgcgcgg
     1321 cagcctgccg cggcgacatt gtcgggcgtt tggagcaggg catcaacggc atggcgatat
     1381 ccgttccccg actaaccagc tcctccaaga attcggttta gctctaatac agatccactt
     1441 gctgccgttg tcccgtgcct cgctaccgaa gcctggaacc tttcatgaca caggcatgtt
     1501 cccccgggta caccacggaa actggaccag gaactccgac gccaattctt tggatctcag
     1561 cgggtgtttc atactggaat ggctccattt tgccctacgc aagtatccgg cacaagaggg
     1621 cgactgcccc aaaatttcat cgggaaatgg cgcttcaacg tgcggcacgt tcggtggaaa
     1681 ctggcggagt ataataaacg cgcatgaaca acttggtcac ggacagcgag caatcggcag
     1741 gtcttgccga tgctgccgaa cctgaaggag agggaaacca tggctcagat tctctggatc
     1801 atcctggtcg ttatcgtggc gatctggctg atcggactct tggcagacat tgcaggaagc
     1861 ctcattcacc tgctgcttgt tgtcgccttg atcatcctca tctacaacct ggtggtagga
     1921 cgccgggcgt agaggatcgc cgtggagcga gaacaacaga agggcgcggc ttatgccacg
     1981 cccctctgtt gtttgctacg ggtacgttcc ggatcctgtt actgttgctg gttgccccgg
     2041 ccagtctctt tctgcaattc atcgatcatc tcggaagccc gcgccttggt catcgactcg
     2101 tccaggtccg cgtcggactt gcccgcctgt tgcaggagcg tccgtagata cgacgcctgt
     2161 ggaccggtca ttggctcgtc accggttgtc cagtcctcag ggtccttcac gggattctgc
     2221 tgcagattct gatcctgctc gttgttgtta tcagacatga tgaccttgta ctccttgctg
     2281 cgtacgtgcc gtttgcctct cctgtcaggc aaaatccctg ccattacttt tgccgcaact
     2341 acgatgaggg caaggatgga gggaggtcaa cgattggatc ctcttgggac tttggagaac
     2401 cgcgccccat gggcaagtcc cggatcaacc atcgctgctt atccaggcca cggtgatcga
     2461 gaaattggcc acagatatac cgtggacctc atgtatcttg ggccatttct ggattgcgct
     2521 tttgaagacg ggcgatagcg gctggcaccg cccgttaccg tcgtgaacgg cctccatgcc
     2581 agggtgaact ccgcctgctg catcggcacc aatgcacctc attggcaaaa tgtcgtatac
     2641 ttgggttcag acgcacgttc atttcgatat gatcggctga tacggcgttt catcacgctt
     2701 gggtcaaagg acaccgggct tcgctatctg gcgcagagcg ccacagtaca agtacaggaa
     2761 acatcacatg ccaacaggaa cagtcaagtg gttcaacgcc gagaagggct tcggcttcat
     2821 cgcacgcgat gagggtggcg acgatctctt cgttcatcgc tccgttctcg gtctcgagcc
     2881 actaggcgaa ggcgatcgcg tcgagtttgt cgaaggaatg ggtccgaagg gtccgagcgc
     2941 cgcatccgta acggttcttg aggccaaccc gaatccgccg gctccgagcg agggttcttc
     3001 ccgcgcaccg gtcgatgtct ccaacctccc ggtcgcaacc ggtacggtcg atcgcttcga
     3061 cgaaatgcgt ggcttcggct tcattcgccc gaccgccggt ggtggcgacg tcttcttcca
     3121 ccaatcagcg gtcaacggcc ttcgcccacg agttggcgaa gaggtcgagt accgtctcgg
     3181 tgagggcccg aagggtcccc gtgccgagag cgtcagggcg ctgatcgatc aggacgagtt
     3241 ctactagcct ccgctggtgg cgcttgccag aacctgaaca accaccctgc gggtaaccct
     3301 gcagggtggt tttctattgc agccggacag tgcagggctt cttcattccg ggatgtcgat
     3361 acagtattgc gatgaattag aacgcatctc ccccacccca ccttcggcct ggcaccggtg
     3421 gacgaaacgg caatgcgcca cctcatatcg tcactggtat gcgacggact ccatcagctt
     3481 gttgccattg acgatcgtcc accatccgcc caggtcgtgc ggacccgtgg agcggcctaa
     3541 cctggacctg aataaggaat tcccacatgc cacgacccct ctctgtcctg gatctcacgc
     3601 gagtgatgga gggcgaatcc tcctcggagg ctatcggcac caccgttgcc atggcccatc
     3661 ttgccgagga actcggttac actcgcatct ggttcgccga gcatcacaac gcgcccggct
     3721 tggccagcgg tgcgcccgaa atcctgattt cgcatgttgc gagccggacc tcgcatatcc
     3781 gtgtggggtc gggcggcgtc atgctgccaa accacgcgcc gctcaagatc gctgaggtct
     3841 tccgtctgct ggaagcgctg cacccgggac ggatcgatct cgggctcggg cgtgcacctg
     3901 gcacggacca gaaaaccgcc tatgcactcc gccgtaaccc ggaggccatg tcggccgatg
     3961 actatcccca gaacttggcc gaactactgg cgtacgagga ccggagcttc ccgccgaatc
     4021 atctcttcgc cacgattcag gccatccccg tcgatgtccc tctgccaccg atctggttac
     4081 tgggatccag cgcattcagc ggcatcctcg ccgcgcagac cggtctcggc ttctcattcg
     4141 catcacacat caatcgcccg gccgccgtgc aggtgatgcg agcctatcgc gaacagttcc
     4201 agccatcggt tcatttcgaa gcgccgaacg ctatcctcgc ggccagcgtg gtcatcggcg
     4261 aaacaatgga gcaggcagag gacttgtcga ccatcgtgaa ggtaggcatt taccgattgg
     4321 tgtccggcca aatgggcccg gctccctctc ttgaggacgc ccgcaaggtc gagatcccgc
     4381 cgcacatgca aatccagatg ggcagcatgc tcggcaacca gtttgtcggc acacctgact
     4441 cagtggcacg tgagctaaag gagtttgccg accaagcggg cgcagacgag ctcatgctca
     4501 caacctggat ccccatgcgc gagaaccggg agtacgtcct tcgggaattg aaacgcgcat
     4561 gggatagtgt cgacgatcgc ggagtaacgg caaactgacg tgcgaagcac acctgatgtt
     4621 gtgcattgca cgaactgacc gggaagcagg ttaccaggta gtctccgcac ctgccgcccc
     4681 gaaatctttg agagaaccag gcggccaaag gccaccggtg cggcctgaat cgcaccctct
     4741 ggccgcgttc gattccacgc tccactttct ggacggcacg gtaggccggg acacgcgccc
     4801 gctcacgccc ctcggtggca gccggggcat ttcttgactc aggctaatct ggaaacactt
     4861 ccgcgcttca tcagagccgc ctgacacgag tactacagcg ttaagaggct gaccaatcaa
     4921 tacatgcgat atcggcgagt cgcatgaaca tgctggtcgt tcgtccattt gtcctccggg
     4981 tcctctgact ttggtctgat ttggaaatgc gcatttggac ggatgttcct ggctttcatg
     5041 aagggcacac tccagatacg ttcagggacc agagctaaga acagacgggc gttgctccta
     5101 cggcacgcac gcgtacgaaa ggacaggcca tgcttccctc gactgcagat acattcgacg
     5161 cgtccggggg catgcacgcc gctcacggag accccggagc gacacgatcg accgggtcaa
     5221 tcgtcggtca atactcgctg agccagatcc ttggaatatg ggcagctgcc gtagtgccaa
     5281 tggttcttct tgcttgggtg gcaacccccc ttgtcgggga ccgcctcaac ctgggtgtgg
     5341 gggatgcgaa ccgggaggcc ttcacacgtg caggctttat cacactcggc ctggtctggc
     5401 agttcgtgct ggtgatgatc atcgtcaagc gtgaagaagg cgatctgggc tggtccacaa
     5461 tccgcagacg gtgctggctc aacaccccgc acgaccccca cactggagaa acgcgccgga
     5521 agctgtggtg gtggttggcg ccattcattg tgggcgtgtt ggcgattcag ctcattccgc
     5581 tccaggcggt ttgggagtgg atgttcccct tcctcgccga gcccgacaag tacagctttg
     5641 ccgagctcct gcagtcggag gagcgaaaag aggcgctcga aggtgcctgg caggtgttag
     5701 ggctcttcct cgtgctgggg ctcttcaaca cggttatcgg tgaagagttc ctcttccgcg
     5761 ggatcctgct tccgaagatg catggcgtct ttgggaaaca cgactggatc gcgaacgggc
     5821 tgctcttcgg cctctaccac ctgcatcagc cgtggtcgat cctctcatcg atggtacggg
     5881 gcgtcttact ctatgccctg ccctccaggc ggttcaggag cgcctggttc ggcatcatcg
     5941 tccactcgat gcagacggtg ttcttcatct ttgtggcact tgggctcgtc ctcggtttgg
     6001 cataggcgcg aaaaaccggg ttacgtccca aggccggggg cgggtgggcc tggcacgggg
     6061 aggctcccct ccatttgccg gcaccgaact tcccttggct tgttatggaa ctgacacacg
     6121 cgccatagtc gtagacgttc agcccgtgca ggtgaaccag aggttcacct gcacgggcga
     6181 tgacgctaca cggtgaatct tccctctgcg tcattccagc ccttgaacgc ccccagcgcg
     6241 gcaccgaccc cggcaccaag gttcaccttt atccccagat catccagagc gcgctccagc
     6301 ataccgacct gggccaccag cgtcgttggg tgcgcggaca ttcccatatg cccgagacgg
     6361 aagagcttgc ccgacagctc cccgtagcca cccgagatca tcaccccata ccgctgtcgc
     6421 atcgtctcgc gcaaggcatc gtccgtgatc ccctccggaa tccgaaccgc cgtgcaacac
     6481 gatgtcgcga tctccggcga ctcgggccag aggtccagac caagtgcgtt cactgcggct
     6541 cgcgctgctt tcgcgctcgt ctggtgccgg tccacaaccg tctccattcc ctctcgtagc
     6601 gcttgcccca gcacagattc aagcgcatag atatcgctta cggatggcgt atatggaaac
     6661 cggcgctgct caagccaggt cgtcttccag tcgagcatcg agaggaagct cccgcgcagc
     6721 ggctccttct tccgttccat tgctgcccac gcatccgcgc tgaccgcgac aagggagagt
     6781 ccggggacac cgccgaggca cttttggggt ccggcaaccg cgatatccat cccccactct
     6841 tccggcgaga acagctccga tccgaggcct gagacagtgt ccacgatcgt gacgacgccg
     6901 aactccttcg caatcctgca gatctcaccc accggattga tcgtgcccga aggtgtctct
     6961 gaatgcacta ctgagagcac agagatatct ggctgggtct gcaggagacg acgaacgtcc
     7021 tcagggtcga tggcggcgtt gtacgggacg gccagctcaa ttggttcgcc gccataacgg
     7081 cggatgaaat cctcgtacca cttcccaaac acgccggaga cgagatttag gaccctcgta
     7141 cccggctcaa tcaccgatgc agcggccgct tcaaggccga gaatagcctc cgcctgtaag
     7201 atgatgacgt cgttcgacgt tcggaatacc tgcttcagga gctccgaagt ccgtgcgaat
     7261 gtttcgatga agacagggtc atagtggtag accaccggct gctgcatggc acggagtgtc
     7321 tccgttgtca catccacagg tccagaagca aggttcaaac gcggccattg ggtcatgaga
     7381 gttctccttc aagctcgaac cgagcatcgt ctgctggaag tatgatccac ggagcgattg
     7441 aaactggagc atcgccacaa gagcgtatct gtcaaaatag cgtggaagta cccgtctagc
     7501 ttttcaccac ggaaggaaac cgcctgatgc caaggattgc cgcgaacctc acgatgctct
     7561 tctccgaatc cccttttctg gagcgcttcg accgtgcggc cgcggcgggc ttcaccgctg
     7621 tcgagttcca attaccctac gaacatgatc cgaacgacat cgccgacagg ttgagccgaa
     7681 acaaccttga gctcgtgctc ttcaatcttc ctgctggcga ctggggtgcc ggtgatcgcg
     7741 gcgttgccgc gatctactcg cgactggctg aattccagga gggcgtcggc aaggcactcg
     7801 agtacgccgc cgtcctgcac cccccgcttc tcaactgcct ggccggcatc gccgctccca
     7861 cgcccgagaa cgacctcgcg ctcctccaga acgtccgctt cgcagccgaa cagctcgagg
     7921 agcatgggat ccgcctcacg ttggaacccg tcaataccca cgacgtcgag agctttgcac
     7981 tgcctaccag ccgctccgtg atcgagctca ttgccgagtt ggagcatccg aatgtcggtt
     8041 tgcaactgga cgtttaccat tcgatcagga tgggcgagga tccgtttgca ttgatcactg
     8101 acctcgggaa tgagattgcc cacatccagg ttgccgatgt ccctggacgc caccagcttg
     8161 ggactggcga ggtggacttc cgtaagctgt tcgacaccat cgatgcaagc ggctatgagg
     8221 gttgggtcgc cctggaatat atcccggaag gtcctaccga ggagggattc gggctgctgc
     8281 gtgagattgg gttgctgccg caataacgca gcacgccgcc gcgctgttac ggagccgcta
     8341 gacgagggat ccagacgtgg acgcagcaca acgattggcc gagtttccaa ccctgcacgt
     8401 cagcaaccat ccgctgatcc ggcacaagca gaccctcctt tcggacgagc ggaccgatac
     8461 gcgcatgttc cgggaactcg tgcgggagct cacgtcgctt ctcgtctacg aggcgatggc
     8521 cgatgttccg ctcaccacct tccgctatcg cacgccgctc gaggagacga ccggctttga
     8581 gctggagccc cgcattggtc tcgtgccgat catccgcgcc ggtctcggta tggtcgaagc
     8641 tgccgcggat atcatccctc actcagaggt ctggcacctt ggcatgtatc gggacgagca
     8701 aacgcacgag ccggtctctt actacaacaa gctccccaag acatgcccgg atgacctgat
     8761 cgtcgtgctt gatccgatgc tcgcgaccgg tggctccgct cgcgatgcca taagagtgct
     8821 caaggcatgg ggggcgaaat ggatcaagtt tgtcgggatc atcgctgcac cggagggcgt
     8881 tcacgagctg cagcaaagcc atcccgatgt cgatgtttat gtttccgtgc tggaccgcga
     8941 gctggatgag aatcgcttca ttcttcccgg cctcggcgat gccggtgacc ggctcttcgg
     9001 aacggtaagg caggagcagg agtgatccac tcacttccct tgatcgttat catcacgacc
     9061 ggcggcacca ttggtagccg gctcgacctg accaccggtg ctgtctcggc tgccgagaca
     9121 gcagaggatt tgattgccct ggtcccggat ttgccagcca tcgctcgcat tgaactcaag
     9181 ccgtggatct cggtcaatag ctggaacata tcgccggcaa tgatgttcga cctgttgcag
     9241 gaggtccgtg ccacactgga caggccagag gtcgccggtg tggttatcac gcatggcacg
     9301 gacaccgtcg aggagacggg cgtcatggcc gagttactgg tcgagtcgga aaaaccggtg
     9361 gtctttgtcg cggcgatgag gaatctctcc gagctgggtt cagagggtcc ccgcaatttg
     9421 ctcgacgccg tgtcggttgc agcagatccc gattcaatcg gtcgtggggc gctcctggtc
     9481 gcgaacgaaa ccatccatgc ggcgcgttac ctcgtcaaga cgaacaccac caaccctgcc
     9541 tcctttctct cgcccgacta cgggccgatg gggctgatcc cggcgaccgg ccttcgttac
     9601 ttacacgccg cgccaaagcg ccggatcctc aatgtaccaa tgctcggcgg gccagttcat
     9661 gtggttaaag ccgtctccgg ttctgactcg tcgcttctgg actggctgat cgatcagggg
     9721 acaaagggca tcgtgcttga gggctctgga gcggggaatg tgccggccgg gatgctgccg
     9781 ggcgttgagc gagcgattga tgcggacatt cccgtcgttc tcaccacacg tgtgctctgg
     9841 ggctttttgg cggcaacgta cggatcgggc ggagcacggg gaggtggatt tgaccttcag
     9901 cagatgggcg tcattccggc gccacatctt ccctcgccca aggcccgtat cgttctcatg
     9961 cttgcgctgg gcgccatact ctcgctggac gagatccggg atctgctggc ggatccctag
    10021 cgaagcgatt cgggccgatg cagtagctca accgccccgg ctatcgagca gtcgctcgat
    10081 atggccgcca tcgtccccgg caaagatctg gtccaggtcg atctcatcct tgaggaagag
    10141 atccacgcct gcctcctcca gcgctgcccg aaatcgacca acgagcgaga gctctctctc
    10201 gttcaggtca tacccgtggc gctccagcgc accatcgaga tcggcgcccg cctccgcccg
    10261 gaatgcctga tcgctcatag cctgctcagc caaccgcttt agcagcggca ttctaatctc
    10321 gtcgtgtgcc atctcgtgcc tttatcgtgt ttcagtcggg atccgcctcc aactggcgac
    10381 gggaccatcc ctgttcgtac agacacgcat cgcagggagt gtaccgcgca ccctgtgcgg
    10441 ggaaggtcgc tatatgctcc gggcctgagt aactgcatcg accagcgcac gcgcacgctc
    10501 cgtcagcagg gaccagtcct tgtcggtgac ggtctttgca tggactaaat tgcttccgat
    10561 ggcgacgcca gcagcacctg ccttgataaa ctctgcacag ttctcgagcg agacgccgcc
    10621 tgtgggtatg atcgggatct gcggcaacgg tccatgcaca tccttgatgt atgttggtcc
    10681 gacagcgctc gccgggaaaa gctttacgaa tgtcgcgcca ccctcccatg ccgtcagcat
    10741 ctccgtcgga gtaaacgcac cgatcaccgt cggcacatcg tagcgctggc acatccggat
    10801 cgtctcgatg ttagtcgttg gggtcacgac gaacgacgcg ccctgcagca tcgctgctct
    10861 cgccgtctcg ggatcgagca cagttcctgc ccccaccgcc acagcgtctc ccatcacatc
    10921 gacaaccgag gagatcgcat tacctgctcg cggattggta taggtgaact ccaccgcgcg
    10981 aatcccaccc tcgtacagcg cccggaccat ctccacagct gcggaatagt cgtccagacg
    11041 gacaatggcg accactccgg gatgactcag ctggtccaac actcgcttca tttgaatccc
    11101 ctcggcgata ggccaacccg ggctggtagg taggggccac tcccctgttg tctgaaatcc
    11161 tgagggaata ccatgccagt ctccctcagg atgtggtcga cacaaagcac tatgcctgtt
    11221 ctgcctcgag cgccgccgcc gctttctcga gcacaccttc ctccgcgagt gactcaagct
    11281 cggagacgac gcgatcgaac tcggctacac cagactggat tggcgcgggt gtctccagat
    11341 cgacgcccgc agtattaagc acattcatcg cgtaatccga cgatccggcc gacagcattg
    11401 ccaggtaacg ctcgcgggct ggatcgccct cgtcgcgcac ggctgtcgcg atattgatcg
    11461 ccgaagagag cccggtggcg tactgataaa cgtagaacgc gttatagaag tgaggaatcc
    11521 gtccccactc gatcctgatt ccctcgtcga tatcgacacc cggtgtatag ctcccactca
    11581 ggtcgctgta aaacgcgctg agcacgtcgg gtgtcagggc gttgcctgcc tccactaccg
    11641 cgtgcgtcat ctgctcgaac tcggcgaaca tcgtctgccg aatcactgtg ccaacatagg
    11701 tatcggcaaa cctgttgagc aggctgaacc gctgcaccgg gtcccgaccc tcgtcctgtg
    11761 cgagcagatg ccaagacagc aggacctcgt tcacagtcga cgcaatctcc gccaggaaga
    11821 tgctgtagcc cgcgtactga atgggctgtg ccttctccgc atagaaacta tgcattgcgt
    11881 gcccagcctc atgggcgagc gtaaagacat cagacagcgt tgagttccag ttcatcaaca
    11941 tcaccggcgg cgcaccgtac gcaccccagg agtacgcgcc cgaccgcttg ccctttgtct
    12001 cgtgaacatc gacccagcgg gattcgaacc cctgccgcag gtcgctcaca tactgctcac
    12061 cgagtgctcc gacacccttc agcacgattt ccaccgcatc ccggtagcca tactgcttgc
    12121 gggattccgg ggccaggggg acccgaagat cgtatcgctt gagttcatca acgcccagtg
    12181 cccgccgccg cagttccatg taacgggcga ctatcggaag tgcagagtca cgaacggtgt
    12241 tgatcaggtt ggagtaaacg gttaccggta cgttgtcatc gaacagcgcc tgctgctggg
    12301 cggactcgta gccacgaaga cgcgctccaa cgacatcctt acgcacggat gagccatgca
    12361 gcgtcgccag ggtatacgtg tggtccagat aaggcttggc gaacttctca gccgccttcc
    12421 gccgaacctc acggtcacgg ctctccatca gctgcccgta ccgacccttg gtcagcgtca
    12481 cctcgttgcc gtcctcgtcg acgacggtcc cgtattcgat atcggcatta tccaacgccg
    12541 agaacgcctc gcttggcgcc ctcgttacat ccgccatctg ggccaaaatt tcttcgatct
    12601 cggctgacag gacgtgcgcc cgttgtcgct ccagatcctc aagtaggtgc ctgtacccga
    12661 ccagctcagg gctctcgatc gctacctgca aggcgtctcc cggcaagctc accagctccg
    12721 gttggacgaa tgccagctcc tcgcctgcct tgatcgagag cgctactgcg cgctcatacc
    12781 gcgccatcgc ctcgggattc gtctggtcct catcccggcg gagcgccgcg tacaccatga
    12841 gcacctggag cctctgttgc gcctcataga gtgcgtccag cgcctcccgg atcaccgtgg
    12901 gaccctcgcc gaagcgccct cggtaggaag cgacgcgagc caccttgtcc ggagcttcct
    12961 ccgcggcggc atcccacgcc tcgtccgacg tgaacatctg cgacaaatcc caggtgtcct
    13021 cgatagcgac atccgcgcgt gtcagtacat cagtggcagt ttgatcgatc attcgtctcg
    13081 tcctttaagc gtgcatgctg gattccatcg aaccggtact gtatagtcat tcgcccgtat
    13141 ccacgacgtg cgacatcgtt gtgccacttc caaagtgggc cacggtcggg atcgacagcg
    13201 cgccgatgac tgcatgtgca ccctaccacg gggtaacacg catatgcagc attcccgcgt
    13261 gcgccgatta aggtccgttg cgtttcgcca ccgcgagggg gagggctctg acatgcggat
    13321 ttacttcgag cttgccctgc gtgccttccg acaactcttc gcgtaccagg ctgcaatgtt
    13381 ggccggcatc ttcaccaact cggttttcgg ggtcatgttg tctgccgtgt acctggcact
    13441 cttccgttcc catgctgatg ggacatcggt ggaaggattt tccgcgaggg agactgtgac
    13501 gtacgtctgg atcggccagg cgctcattgc ccctgtcatg atctggggtt ggtgggagat
    13561 catcaagacg atccgcaccg gtgctatcgt gacggatatg ctgaagcccc tgagcttctt
    13621 tggcttctgg cttagtcgtg acctgggccg cgcgatgggg cacctgcttc tgcgaggcgt
    13681 gcccacattg attatcggcg ctcttttcta tgatctcgtc taccccggta caccgggaag
    13741 atggctcgca ttcctcgtct caatcccatt cgccgtgatt gtcagcttct gcttccgctt
    13801 cataatcaat ctctggggtt tctgggtcct ggatcatcgc ggcatcgcag gaatcgcgat
    13861 agtcatcgtc ggcgttgcct caggacacct tctgcccatc gcgtggtacc cgaacgcctt
    13921 ccgtgatgtg ctcaacatgc tccccttccg ggccatcgtc atgctccccg ttgagatctg
    13981 gctgggccag gtaggtatcg tggagggtct gggcctgcag gtattctgga ttctcgtcat
    14041 gacagccgcc gcgcactggt tacagtccgt ggcagagcgg aaagtggtgg tacagggtgg
    14101 atagtatctt gacccttcgc caggaatgtc gcatcgccat acgcattgct ggcatacgac
    14161 tccgcggcca gatggcatat cctgcatcgt tctggatgca gatcttctca agcttcgtga
    14221 tacatgccgc ggagattttg gccctgttgg cgatgttcaa ccggttcaat gacatgggag
    14281 gatggaccct cggcgaggtc gcgtttctcc acggcatcag catgacggcc ttcagcatcg
    14341 gcgataccat cgctgtcggc ctggacagcg ttcccaacca gattcgtgag ggcgaattcg
    14401 accgcgtgct catccggccg ctctcctcat ggatccagac tgtcgtcagc gaagtctcgc
    14461 tccggcatct gggccagtgc gcccagggcc tcgtcatact tgggtttgcg ttgacctgga
    14521 cggacatcca gtggacgctc cagaaagcgc tcttcatgcc cgtggtcatc gccacagggg
    14581 tcgccctctt cgttgccctg tttacgctgg tggccatcct gagtttctgg acggtcaact
    14641 ccatcgaagt tgtcaacgcc ttcacctacg gtggatcgga tctcgcccag tttccgctcc
    14701 acatctttga ccgctggatg cggaacctct tcctctttgt gattccagtc ggattcactg
    14761 cctactaccc tgccctctac attctgggga aagacgaccc gctgggcatg ccagacacct
    14821 ttaggttcct tgggccggtt gtgaccgccg gcttttgcct ggtcgtcgcg tggggctgga
    14881 accagggaat ccgtcactat cgatcgaccg gctcctgaac caggaaggct tcaacgagga
    14941 atcaggagcc cgcggtcagt tcaccagcag tgctatccac gcaatagatc cattcaaagg
    15001 tcgtacagaa aggatgaggg catgcacatg gatggaccga taatcgaagc gagaaatctg
    15061 gagaagcgct tcacgatacg acggcaccgg gagggagcat ttggtgccat gcggagcctc
    15121 gtcgatcgcg gcggcaatgc ggtttccgcc gtcaaggacg tctccttctc aatcaatcgc
    15181 ggcgagatgg tggggtatat cggcccgaac ggcgccggca aatccacgac catcaagatg
    15241 ctcacgggca tcctcgtgcc ttcatctgga gaggccacag tcgccggctt cactccatgg
    15301 gagagccgaa agcagctcgc gcagcagatc ggtgtcgtct ttggccagcg tacgcaactc
    15361 tggtgggacc ttgcattgac cgactccctg gaactgctac gccacatgta ccggataccg
    15421 gagaagcggt tccgggaaaa cctggatcgt gcccgggagt tgctcgttct cgacgacttc
    15481 atgcatacac cggtccgtca gctctcgctc ggccagcgca tgcgaggcga ccttgccgcg
    15541 gcactccttc acgaccctaa gattctctat ctcgacgagc caacgattgg gctcgatatc
    15601 gtggccaagg cgcgtatccg ggagttcctc gcggcgctca accgcgagac acacgtaacc
    15661 gtgctcctga caactcatga tctcgcggac atcgagcagc tctgcaggcg gattatcgta
    15721 atcgaccgtg ggcaggtggt ctacgacggt gagctgggga ccctccggaa ccgcttcggg
    15781 ggcaagcgcc acgttatcct cgacttcgat caggatccgg gtgaggcaat ctcaaccctc
    15841 gccggaaaca acgtggagat cgtgcatcac aatggaccgc gggtacgtgt cgccttcgac
    15901 agggaaacga caagcgcccc gcgggtcctc gccctggcca gccaccacgg gaccgtgcgc
    15961 gacatgtcga tcgaggaacc tgcaatcgag gacgtcatcc gtcagatgta cgaagggcca
    16021 gacagccacc atccctaaag caatgtgcaa tgagattatt cccaatcccg ccacgcggaa
    16081 ggtgtagcgg acgacgcata ggcgattgcg gagcaattga gcgagagcga ccccccggcc
    16141 gagcccggag ggcgagaagc ggcagcgagc ctttgtcgca gcaatgtcgt cctgtgtgcc
    16201 ctgtcgcgaa gacattgctg cgtcgttgcc gcgaagaggc agcatgctcc acgcgttcca
    16261 tgagctagcc ctttggcgaa aagtgtctgc ggcccgctct cgcaggagag gagccccgat
    16321 agaatgcggc ggtctcatgc gacctcgtcg tctacactat tcttgacgat cgaaaccgcc
    16381 acgctgcccg ggatactcca tgaccgcttc cacccaaatc atctgccgcg actgcaacaa
    16441 cgcattcatc tacaccgacg ccgaacgcga cttccgggaa tcccgggggc tcacgcgccc
    16501 ggagctttgc ccggagtgcc ggggcaagga gcgtgctcgc aggaacgggg atctcatctc
    16561 gctctatcag cgtgtggatg ccctcgaccc attcctcatc agcggtgaaa caccacgtaa
    16621 cggccgcaat caccgcatcc gcgatacccg ccagcagtac acaaccacct gtgccgcctg
    16681 cggcagcgag acccgtgtgc ccttcgtccc gcggggcgac cggccggtct attgccggtc
    16741 ctgttacaac gcacgcaagg gtcgctagta ccgcagccag ccaggaacga atgaagaccg
    16801 ccgctccccg gtatgggagc ggcggtcttc attcgttgca gcgggtaacg ggcaaggatg
    16861 gaaaacgagc cctgtcggtc ctagccctcg atgtccgaag cgaactcctt ctcgacctcg
    16921 gtcaggctct cgtcgacgat cgaaaccatg cgatcgatct cgtccctcgt cacgaccagg
    16981 ggcggcgcga agtggacgat attccatgtg cgggtgatga gccccttctc cagcaccttc
    17041 tgaccgagca ccttgcagaa cttgtcgttc ttgctgaatg gcgtcttggc ttccttgttt
    17101 ttcacgacct cgatcgcctg cagcaggccc acaccgcgta cgtcacccac ggacggatga
    17161 ttgcgcaact cctcgaggcg ttctctcagg tagatcccca tctcggcgcc tcgggagacc
    17221 aaatcctcat cctcgaagat ttcaaggttc ctcagcgccg cagcggcagc tacaggatgg
    17281 cctccaaagg tgagcagatg gcccatcggc acatcgggct gggactcaaa gacctcgaac
    17341 accttcggcc ccgcgattgc cgcacccatt ggcatgtaac cggaggttaa acccttcgcg
    17401 atcgtcatga tatctggcac gaatccgaac tgttccgtgg cgaacatcgt ccccgtacgt
    17461 ccaaagccat tgatgacctc gtccgcgatc agcaggacgc cgtggcggtc gcagatctca
    17521 cgaagcatct gccaatattt gggtgacggc acatgcacgc ccgccgcgct ggagatcggc
    17581 tccccgatca ccgccgcgac cgtctccgga ccttggctga tgatctcctg ctcaacgaac
    17641 ttggcggcca tgatatcgct ggcctcgccc tccagctcga aagggctccg gtactggtcc
    17701 ggcggcggtg cgtaggaaac gccgtacatg aacggtccga agaacttttc ttcgcgcgcg
    17761 gccgtgaggc tcatcgcacc atgcgtcatc ccgtggtagg aaccacgccg ggcgatgatc
    17821 ttgtagcgtc gtgggaagcc gcgcattgcc tggacctgct tggcgatctt catcgccgtc
    17881 tcgacggctt cggagccgcc ggagcagaag taggaccgcg agaggtcgcc cggcgtgatc
    17941 tcggcgagct tgtgggccag gtgtgcggcc ggctcggtcg tgtactggac ggcggagaca
    18001 taggaaagct tcgcggcctg cttcgccatc gcctcagcga tttccccacg gccatgccct
    18061 gcattcacaa cccacaggcc ggcgatgccg tcgagatact ccctaccctc gatatcccag
    18121 agcgtggacc cctcgcctct atcgaagacc ttcagccctt cattctccgc gacatcgacc
    18181 catggcagtt ggtgcggcca gagatgatcc ttcgccttgt cggttacatg cttctgcgtc
    18241 tgagttgcca ttaatgcctc cgctggctgc cccgtcatga gtaccgtcct cctgatggac
    18301 gggtctgtca ggtccctgaa tacgacacct gcatcctacc gcacttccag tggcgcgcgt
    18361 acgatccgtc gctcctgccc gggtgctacc atcaatgctg cagatggcat cctttgtgca
    18421 tcttgccgtt gagtgaaatg tgagcgaatc gaaaggacac caggtgactg catccccgtc
    18481 ctcccgcctt gtcattgatt accggaatgc cactgttgat gctgttgggc cgatgcatgg
    18541 catctcggat gccgagctca acgagcttca gccgtatgtc agcgaacagc acgaccggat
    18601 cgtcgacgag cacaggaaca gcgtgcagcg ctggcaagac ctaccagaca acgctgccct
    18661 cgccgacgat gttgttcaat tcgccaatga tgctcgcagg acctaccgcg acttcattct
    18721 gatcggcatc ggcggctcct cactcggggc catcgcgacg atccaggcgc tctgccatcc
    18781 ctaccggaac ctgctcccgg atgaggagcg tggcggtcca cgattcttcg tgcttgacaa
    18841 cccggacccg gaaaaggtcc gtgcgaccct tgaaacagtc gacctctcca ggacgctggt
    18901 caatgttgtt tcgaagtcgg gacaaacg
//
LOCUS       DASYWM010000182         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_251986, whole genome shotgun sequence.
ACCESSION   DASYWM010000182 DASYWM010000000
VERSION     DASYWM010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251986"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VGR29_07600"
     CDS             <1..616
                     /locus_tag="VGR29_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2073492.1"
                     /translation="ETVRVVEASGRRAVAYPGDIQDEEFCKNLIERTVEDLGKIDILI
                     NNAAFQSTHERFTDITTELWQRAMRTNLDAMFYLSKYALKRMPPEGGAIINTTSIQAF
                     QSSPELTHYATTKGGIVSFTRALAKDAIQQGVRVNAVAPGPIWTPLIVSTMDPEKYTT
                     FGENTPMGRAGQPKELAPAYVFLASNDSSYITGEIIGVTGGNPL"
     gene            718..2358
                     /locus_tag="VGR29_07605"
     CDS             718..2358
                     /locus_tag="VGR29_07605"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEV2073493.1"
                     /translation="MAKMANPTREIRDVASDFLGLDHLRPGQRDAITSVLEGRDTLTV
                     MPTGSGKSAIYQVAGLILKGLTIVVSPLIALQRDQVTSLEDTGTITAAELNSTLTDKQ
                     REEVFAQLEDVDQMFLFLAPEQLANEETLERLGQAKPALFVVDEAHCISEWGHDFRPD
                     YLKLGAFIDKLGHPPILALTATAAGPVRDEIVEQLSMEGPNIFVSDFDRPNIFLNVDT
                     FHDSGTKHDALIEYASGQQGSGIIYTATRKHAEQIAGELSDRSITARAYHAGLSARER
                     ESTQEAFMSGEITVIAATIAFGMGIDKPDIRYVCHYSISESLDSYYQEVGRAGRDGDP
                     AEARLFYNPDDLGLRRFHSGVGDLDAENVRPVLATIRNAGEHVDPAEIREELDLRDTE
                     LIRVIGRLEDVDAIEVAPSGELTPINTDVSVSEVAEAAAEAQLQLRNYASTRLEMMRQ
                     YAEVPSCRREFLLNYFGDPYEGPCNNCDRCRENPEEAAHSEDRPYPLQSKVMHTSWGE
                     GTVMHYEDDRIVVLFGDAGYRTLSLNLVQENNLLEPLT"
BASE COUNT          588 a          971 c          784 g          465 t
ORIGIN      
        1 agaaaccgtt cgtgtcgtgg aagcctcggg gcgccgggcc gtcgcctacc cgggtgacat
       61 ccaggatgag gagttctgca agaacctcat cgagcgcacg gtcgaggatc tcggcaagat
      121 cgatatcctt atcaacaacg ccgccttcca gtcgacccac gagcgcttca cggatatcac
      181 gaccgagctg tggcagcgcg cgatgcgcac caatctggac gcgatgtttt atctcagcaa
      241 gtacgccctc aagcgcatgc cccccgaggg tggcgcgatc atcaacacga cctcgatcca
      301 ggccttccag agcagccccg agctgacgca ttacgcgacg accaagggcg gtatcgtcag
      361 tttcacccgg gcacttgcca aggacgccat ccagcaaggc gtccgcgtca atgctgtcgc
      421 tcccggaccg atctggaccc cgctgatcgt ctcgacgatg gacccggaaa aatacacgac
      481 attcggcgaa aacaccccga tgggacgcgc cggtcagccg aaggaactcg ccccggccta
      541 cgtcttcctc gcctccaacg actccagcta tatcaccggc gagatcatcg gtgtcacggg
      601 tggcaatccg ctctagacgc cctggtactc ccgtgaggac atcgccgaca gacacacgct
      661 tctgtcggcc gtgtcctctc attctgtccc attagccctg tagatgagat atcaacaatg
      721 gccaaaatgg caaacccgac gcgcgagatt cgcgatgtcg ccagtgactt cctcggcctc
      781 gatcaccttc gccccggaca gcgcgacgcc atcacatccg tgctggaggg ccgcgacaca
      841 ctgaccgtga tgccaaccgg ctccggcaag tccgcgatct accaggtcgc cgggctcatt
      901 ctcaagggcc tgaccatcgt cgtttccccc ctgatcgccc tccagcgtga ccaggtgaca
      961 tcacttgagg acaccggcac catcaccgct gcggagttga actccaccct taccgacaag
     1021 cagcgggagg aggtcttcgc ccagcttgag gacgttgacc agatgttcct cttcctcgcg
     1081 cccgagcagc tggccaacga ggaaacactt gagcgtctgg gccaggccaa acctgcactc
     1141 ttcgttgtgg acgaggcgca ttgcatcagc gagtgggggc acgatttccg accggactac
     1201 ctgaagctcg gtgctttcat cgacaagctg ggccacccac cgatccttgc gctcacggcc
     1261 accgcggccg gcccggtgcg cgacgaaatc gtcgagcagc tctcgatgga ggggcccaac
     1321 atcttcgtca gcgacttcga tcgccccaac atctttctta acgtcgatac cttccacgat
     1381 tccgggacca agcacgacgc gctgatcgag tacgcctcgg gccagcaggg gtcgggcatc
     1441 atctacactg ccactcgcaa gcatgccgaa cagattgccg gagagctgag tgaccgaagc
     1501 atcaccgccc gcgcctacca tgccggcctg agtgccaggg agcgggagag cacccaggaa
     1561 gcgttcatgt ccggcgagat aaccgtcatc gccgccacca tcgccttcgg catgggcatc
     1621 gacaagcctg atatccgcta cgtctgccac tacagcatca gcgaatccct ggacagctac
     1681 tatcaggaag tcggccgcgc cggcagggat ggggaccccg ccgaagcccg gctcttctac
     1741 aacccggatg atctcggcct gcggcggttc cactccggcg tcggcgatct cgatgccgag
     1801 aatgtccgcc ctgtgctggc aacaatccgg aatgccggag agcacgtcga cccagccgag
     1861 atccgggaag agctcgacct tcgcgatacc gagctgatac gcgttatcgg ccgcctggag
     1921 gatgtggatg ccatcgaagt cgccccttcc ggtgagctaa ctcctatcaa taccgacgtc
     1981 agcgtctcgg aagtcgccga agccgccgcc gaggcccaat tgcaattgag aaactacgcc
     2041 tccacccggc tggagatgat gcggcagtac gccgaagtcc cctcgtgccg ccgggaattc
     2101 ctgcttaact acttcggcga tccctatgag ggtccctgca acaactgcga tcgctgcagg
     2161 gagaacccgg aagaggcggc tcacagtgag gaccggccct accctctcca aagcaaggtg
     2221 atgcacacaa gctggggcga gggtaccgtc atgcattacg aggacgaccg tatcgtcgtg
     2281 ctcttcgggg acgctggata ccgcacgctg tccctgaatc tcgtgcagga gaacaatctc
     2341 ctcgagcccc tcacctgaca tatcaaaggg agcgttcagc cttcattgtc ctgcccgcgc
     2401 acggtctccg ccgccattat cctgagcgca gcgatgaatc ctgggtgaag ccaggggccc
     2461 aaagcccctc gcaaagcagc tgaccacccc gtcatcctga gcgcagcgaa ggatcccccg
     2521 gcgaagccgg tgccctccga gcgcccgtca aggcgcaaaa cgcccgatac cgaggagctc
     2581 cgcgaaacag cccctcacgc ccatcatcct gagcgcaggc aatggcagca ataccccgag
     2641 cggaatgccc caccaccccg gcatcccgag ggaagcaagg gaacgcaaag ccctgcgcga
     2701 cacggcccac cccccgtcat cctaagccgg agacgcgaac gtcgcttaga gccgtgtcga
     2761 aggagccccg cgcgaagcgc gttggtgctc gatgtcatac ccctccgc
//
LOCUS       DASYWM010000183         3997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_307771, whole genome shotgun sequence.
ACCESSION   DASYWM010000183 DASYWM010000000
VERSION     DASYWM010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307771"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="VGR29_07610"
     CDS             complement(<1..1331)
                     /locus_tag="VGR29_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV2073494.1"
                     /translation="MRNLLAPFRWFLHSGIVRSLRFRLILTVLLASLPTLLLLFLTAS
                     QQRAEALEFGQQEAERLARTAAADQGREMDRVQRELALLTRLPEVQSHDPSPCTGFFQ
                     GLVIDPDNAIYVDLRVLTEDGEVFCRSTGASPLRQNEDLGFIEDAFDGTEFTVGPYRV
                     NPANGNAIISFAAPVRDDDGTIERVVVATLDLGSQSTFMTQSTLPEGAVIQLVDEQGT
                     LLLQRPPQRDVLGASLRGTPVVDEAIGIAATPVPGGGDDGDDGDEVMVEDDYITATQP
                     ITITSTDSVAGNASVIVLVPEEAIVRGANDAFRDNLGKLGVATAVVIFAAWISADLFV
                     ARDGETRKSIVAELYHAYSSGAVEHLDGIVAADFTDHSPAPGQSKGIDGLKQSVAAFR
                     TAFPDGEIVPREMLADRDKVVARVSLTGTHLAEFQGVPPSGKRMIADGTET"
     gene            complement(1594..1995)
                     /locus_tag="VGR29_07615"
     CDS             complement(1594..1995)
                     /locus_tag="VGR29_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2073495.1"
                     /translation="MSQAQQRHHILCINNSKEVLELFRELLEEEGYRVTTQSYVDKDL
                     DAISALKPDLIVLDYMWDHEDSGWALLQMIKMSPQTENIPIVLCTGAVRQVNELEAHL
                     LDMQVVVVFKPFKLDHLVQTIRSRLNVAPES"
     gene            complement(2951..3442)
                     /locus_tag="VGR29_07620"
     CDS             complement(2951..3442)
                     /locus_tag="VGR29_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2073496.1"
                     /translation="MMEPSDQKHILAMNESDDVLKLFRELLEDEGYRVTMQPYLDKDL
                     GRIEVTAPDLILLDYMWAGDDAGWALLQMLKLHPATSQIPILLCTGAVKQVEGLKTHL
                     HDMQVRVIYKPFNIEELTREVAAALEAAAPEPEHLAGSPDVDSLSANTNHVHEHGDPV
                     VNA"
     gene            complement(3673..>3997)
                     /locus_tag="VGR29_07625"
     CDS             complement(3673..>3997)
                     /locus_tag="VGR29_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEV2073497.1"
                     /translation="SAIHVRTFYMTDRPSLEASDDSPPKPTQVRGTPLPDELLHQLGT
                     PLTIISGHTQLLQRRTRHMIGDDAATLDRSLRAIEGAVQRMVTVLMKGYTQDDSEKSV
                     DEDTN"
BASE COUNT          804 a         1217 c         1132 g          844 t
ORIGIN      
        1 aatgtctccg tgccatcggc aatcatccgc ttcccactcg gcggcacacc ctggaactcg
       61 gcaagatgcg ttccggtcag cgagacgcgc gccaccacct tgtcccgatc ggcaagcatc
      121 tccctcggca cgatttctcc atctggaaac gccgtgcgga aagcagccac gctctgcttc
      181 aggccgtcga tccccttcga ctgcccgggt gccgggctgt ggtccgtgaa atcagctgcg
      241 acgataccat cgagatgctc gaccgcaccg gaggaatagg cgtgatagag ctccgcgacg
      301 attgacttgc gcgtctcgcc gtcccgcgcc acaaagagat cggcgctgat ccacgcggca
      361 aagatgacta ccgcagtggc taccccgagc ttgccgaggt tatcccggaa ggcatcgttc
      421 gcgcctcgca cgattgcctc ctccggcacc agtacaatca cagaggcgtt gcccgccacc
      481 gaatcggtgc tcgtgatcgt gatcggctgg gtggcggtga tgtagtcatc ctcaaccatc
      541 acttcgtccc cgtcatcacc gtcatctcca ccaccgggca caggcgtcgc cgcgatgccg
      601 atcgcctcgt cgactaccgg cgtcccacga agggaagccc caaggacatc tcgctgcggg
      661 ggacgctgca gcagcagcgt accctgctca tcaacgagtt ggatgaccgc accctccggc
      721 agggtggatt gggtcatgaa tgtgctctgg ctcccgagat ccagtgtcgc cactaccacg
      781 cgctcgatcg tgccgtcatc atcccgaacc ggtgccgcga agctgataat cgcgttcccg
      841 ttcgccgggt tcacccggta gggacccacc gtgaactcgg tgccatcgaa cgcatcctcg
      901 atgaagccca gatcctcgtt ctgtctgagg ggacttgcac cggtgctgcg gcagaagact
      961 tcaccgtcct cggtcaaaac ccgaagatcg acatagatcg cattgtcggg atcgataact
     1021 aggccttgga aaaacccggt acagggggaa ggatcgtggc tctgcacctc cgggagccgt
     1081 gtcaacagtg caagctctcg ctgcacacga tccatctccc gtccctggtc cgccgcggct
     1141 gtcctggcca gacgctcagc ctcctgctga ccgaattcga gagcttcggc acgttgctgg
     1201 gacgcggtga ggaacagcag gagcagcgtg gggagcgacg caaggaggac ggtcaggatg
     1261 aggcgaaaac gcaagctccg gacaatgccc gaatgcagga accagcgaaa cggggccaac
     1321 aagtttcgca tggctctcct cctcgctcta tcctgcttga cgcaggtcct cgctccgcct
     1381 ccgggtggat gttaaccata ccgcagcggg gctgatgcag acagggggaa tcttcccgtc
     1441 acccggatga gatagacgga cccggccaat gcgcaccccc cgtgagccgg ggaccatcgg
     1501 tgtctctcac cgagctgtgc cggaggcata gaggggctgc cgccttggat gcatgccctg
     1561 cgaatcggct tatgaggatg tctggtcgga catttacgat tcaggtgcga cgttcaatct
     1621 gctcctgatc gtctgcacga gatgatccag cttgaatggc ttgaacacca ccaccacctg
     1681 catatcaagg agatgcgcct cgagctcgtt gacctgcctc acggctccgg tgcaaaggac
     1741 aatgggaatg ttctcggtct gcgggctcat cttgatcatt tgcagcaatg cccagccgga
     1801 atcctcatgg tcccacatat agtcaaggac gatcaaatcc ggcttgagcg cactaatggc
     1861 atccagatcc ttgtccacgt acgactgggt cgtgacgcga tagccctcct cttcgaggag
     1921 ttcgcgaaag agctcgagga cctctttgga attgttgatg caaaggatgt ggtgcctttg
     1981 ctgtgcttgt gacattggga tagccttctc ggcgaccgag tgctgaaact cggccgtcgc
     2041 gttgcaggcg catccctatt gcggccacag ggtactcatt ctgccagggt gcaggttgcc
     2101 agagacggca ggaaacgacg actgggcaat ttctcatggt gacgagacgg aggacagtgg
     2161 gctcacttct tccaatggcg tataccttag tatacacacc attctcaata gatcataact
     2221 gtcacaataa aaatacttaa tctattaaga atcagcagga gtatcgtttt tcactcccag
     2281 gcgtacgcgt attggcttct taataattgg aagtggcgac tagaagcatg gggccaacct
     2341 ccagttctcc gcctttcggc aggggaacgg cccgtctgtg catgggcctg gaacttgcat
     2401 acaacaaccg atccgagtcc ccaaacgaac gtggtcgctt cgttggctcc tgatcgggac
     2461 cggcggcccc atcggttccc ggtgtcgcgg tgggctcacc caccggcttg gccgggcgaa
     2521 cgctgtccgc aggtaattca ttcccagctc cggaggcgtc accgagccca tccacaacgc
     2581 atcgggtctg cgtctcttct gcatcgtcgt tggcaaatcg ctgtccaaga ggaacagcag
     2641 ggcgggcgta tcggcccatt cctgtagagg gggagtggag gccgcgccgc ccagtggttg
     2701 gggatgcggt cgtgaaccaa aggtgcacca tcagaacctc agatatcagc ggacaccacg
     2761 ccacaatggg cagcccatgt gctttcagac tgttcaggaa cccactcgca tgctagcagg
     2821 gaccggggcg atgcacaaga gccccagatt gatcgcccaa cactgatccg ccgcgtacct
     2881 ggtgggtctt cctgaaaggc aatcgcgcga cgccaacacc gccacgacgt gggatgctgc
     2941 cctgttgggc ttaggcgttg accaccggat caccatgctc gtgaacatgg ttggtgtttg
     3001 ctgagaggct gtccacatcg ggagagccag ctaaatgctc gggctcgggc gctgcggctt
     3061 caagcgccgc cgcgacttcc ctggtcagct cttcgatatt gaacggcttg tagatgaccc
     3121 gcacctgcat gtcgtgcagg tgggttttga gcccctctac ctgtttgacg gctcccgtgc
     3181 acagcaagat cgggatctgg ctggtggcag gatgcagctt cagcatctgc agcagcgccc
     3241 atcccgcatc gtctcccgcc cacatgtagt cgagaaggat caggtctggt gcggtcactt
     3301 cgattcgccc gagatccttg tccaaatagg gctgcattgt gacacgatac ccctcatctt
     3361 cgaggagttc gcgaaacaac ttgagaacat cgtctgattc attcattgcc aggatgtgct
     3421 tctggtccga tggttccatc atgacatcct tctagggaga tgtacgcagc tcggaccgag
     3481 cgaccggtca cgtcctctat ctctagtatg tgtatgcacg aataggcagg cctgatcgcg
     3541 cctccaaccg gtgctggaat catcaaaccg ctttcgggcc cgtcagcatt cccttaaatc
     3601 ccgtggtcac cgatttttcc cccatgggtt cttggagcca tgataccgta tatcccggta
     3661 tttcccctag tttcaattag tatcttcgtc tactgacttc tcgctatcgt cctgcgtata
     3721 acctttcatt aataccgtca ccatacgttg taccgccccc tcgattgccc tcagtgaccg
     3781 atccagcgtt gcggcgtcat cgccgatcat atgcctcgtc cgtcgctgga gaagctgggt
     3841 atgcccgctg atgatcgtaa gaggagttcc cagctggtga agcaattcat ccgggagggg
     3901 agtgccgcgg acttgtgtag gtttcggcgg cgaatcgtca gaggcctcca aagatggtcg
     3961 gtcggtcatg tagaaagtcc tgacgtgaat agcactc
//
LOCUS       DASYWM010000184         4232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_157909, whole genome shotgun sequence.
ACCESSION   DASYWM010000184 DASYWM010000000
VERSION     DASYWM010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157909"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VGR29_07630"
     CDS             <1..524
                     /locus_tag="VGR29_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073498.1"
                     /translation="IRTRDEVTVVASRRLFARARTPDALATMAPNKIDALIPAVTFHE
                     RKAVQIHDIAIRVRDDFAGELPCEPEVMQSFSGVGPKCANLALGIACNQQRISVDIHV
                     HRVTNRWGYVHTRTPEQTMAALEGVLTERYWIEINERLVPFGKHICTGVRPKCSTCPV
                     LEMCQQVGVTSHR"
     gene            complement(521..1348)
                     /locus_tag="VGR29_07635"
     CDS             complement(521..1348)
                     /locus_tag="VGR29_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2073499.1"
                     /translation="METVPVSTAQPWYDVRRAEPGIFIIEEPFHYEHVKSYLVEGDER
                     ALLLDTGTGAGDLRALVESLTDRPVFLVNSHAHWDHIGCNRQFDERWIHEAEADAATL
                     VDGVGNERLRHAFAPEGLTGPLPEGFDVETFVIPPAVPTGVMEDGHHFDLGGRGLEVL
                     HCPGHSPGGIALLDRANGALFSTDVAYAGALYVYEPANLLTYQASLQRLADLAPTLHA
                     VYPAHDASPIEPAMLPGMAQGVRDAMDGMEPSSRAGDMARYEFDGFVLQVWGMPEPA"
     gene            complement(1360..1482)
                     /locus_tag="VGR29_07640"
     CDS             complement(1360..1482)
                     /locus_tag="VGR29_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073500.1"
                     /translation="MWDEREGFVYRSARFDHRNRDGRYGFTSLIIDAVKPAMTG"
     gene            1604..1879
                     /locus_tag="VGR29_07645"
     CDS             1604..1879
                     /locus_tag="VGR29_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073501.1"
                     /translation="MIDRSWAADLHDPLVRLVREEPIRHGTPNEATGPEEIEADAGSH
                     PGFLKVEVLPDVLGEYPAEAAGPKPFLPVGSPRQIEFLFLGPDTPVR"
     gene            complement(2142..3227)
                     /locus_tag="VGR29_07650"
     CDS             complement(2142..3227)
                     /locus_tag="VGR29_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV2073502.1"
                     /translation="MEDVQAWLRDPRLGREWRRLLPPGEQPPEPEVNSFRDVADHFML
                     FRDPPDHTRLRSTANMAFTPRHVNKMRAPIERLALDLVEDMKRQDGPVEFIASFAYPL
                     PVLVIAGILGVPESDFSRFRDWSAVIAAAINLPVTGLQEFVQRADQTTSELVEYLGWI
                     VAERRKESKDDLISSLIAAEARQGKISEKELISTLILLLVAGHETTVNLIGNGTYALL
                     QHPDQWQALVGDPSLARNATEELLRYDSPVQMTTRTAMEDLEIAGTPVRQGTEVQFVL
                     GSANRDPRAFEAADRLDIGREVGRIMSFGMGIHFCLGSPLARMEGEIAFQTLTQEAPD
                     LRLGATQPEWRAGLVLRGLQTLPVILS"
     gene            3520..4200
                     /locus_tag="VGR29_07655"
     CDS             3520..4200
                     /locus_tag="VGR29_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEV2073503.1"
                     /translation="MSPLSSQQPTGFRDRVDAGARLAMPLLPRVATLAPDDIVVIGLA
                     RGGVIVAAEVARQLDVRLEAIVVRKLGAPDQRELAIGALAASGERVLNQPLINDIGLD
                     QEQVESISSQAQKAAMSLCREIGVPAVVPDIVGKTAILVDDGLATGATMRVAIEAVYH
                     QGARQVFVALPVAPASMVEPFREIADDVITIIAPENLRAVGQWYGYFPDVPSSAVRDA
                     LAANRASA"
BASE COUNT          832 a         1391 c         1227 g          782 t
ORIGIN      
        1 caattcgcac gagagacgag gtcaccgttg ttgcgtcccg tcgcctcttt gcgagggctc
       61 ggaccccgga tgcccttgca accatggccc caaacaagat cgatgccctc atccccgccg
      121 tgaccttcca cgagaggaaa gcggtccaga ttcacgacat cgcgatccgc gtccgcgacg
      181 acttcgcggg tgagctcccc tgcgagccgg aagtgatgca gtcgttctcc ggcgtgggac
      241 caaaatgcgc gaaccttgct ctcggaatcg cctgcaacca gcaacggatc agcgtcgata
      301 tccacgtcca ccgtgtcaca aaccggtggg gatatgtcca caccaggacg cctgaacaga
      361 cgatggccgc actggaggga gtgctgaccg aacggtactg gatcgaaatc aacgagcgcc
      421 ttgtcccttt tggaaagcac atctgcaccg gtgtccgccc caagtgctca acctgccccg
      481 tactcgagat gtgtcagcag gttggggtta ccagccaccg ctaggccggt tccggcatcc
      541 cccacacctg aagcacgaaa ccgtcgaact cgtagcgcgc catgtcgccc gcgcggctgg
      601 acggctccat accatccata gcgtccctga ccccctgcgc catgccgggg agcatcgccg
      661 gctcaatcgg gctggcatcg tgcgccgggt agactgcatg caatgtcggt gcgaggtccg
      721 cgaggcgttg cagtgaggcc tggtaggtca acaggttggc cggttcatag acgtagagag
      781 ctcccgcata cgccacatcg gtgctgaata gtgccccgtt cgccctgtcc agaagagcaa
      841 ttccgccggg ggagtggcca gggcaatgca gcacctcaag cccgcgccct ccaaggtcga
      901 agtgatgccc gtcctccata acgcccgtgg gaaccgccgg gggaattacg aaggtttcga
      961 catcgaaccc ttccggcaat ggaccagtca ggccttcagg cgcgaacgca tgccggagcc
     1021 gttcattacc tactccgtcc acaagcgttg ccgcgtcagc ctccgcttcg tgaatccatc
     1081 gctcatcgaa ctggcggtta caaccgatgt gatcccagtg ggcgtggctg ttgacgagga
     1141 acacgggcct gtcggtgagt gactcgacaa gagcgcgcag gtctccggca ccagtgccgg
     1201 tatccagcaa cagtgcgcgc tcatcccctt ctacgaggta ggacttcaca tgctcgtagt
     1261 gaaacggctc ttcaataatg aagatgccgg gttcggcgcg cctgacgtca taccatggct
     1321 gtgctgtcga tacgggtact gtttccattc cgctcctctt catccggtca tcgccggctt
     1381 aaccgcatca atgatcagcg aggtgaagcc gtaacggcca tcgcgattac ggtgatcaaa
     1441 gcgcgccgac cggtagacga acccctctcg ttcgtcccac atctctgcat gaaactcgcc
     1501 cttcgcgtcc cacccactcc agcagctgca cctcgaaccc ggcggccgca aacaatgcca
     1561 gcaacgagcg gtaagcgtag acgaccctgt ggccggcagc gggatgatcg acagatcctg
     1621 ggccgccgac ctgcacgatc cgctggtacg tctcgtccgg gaagagccca tccggcacgg
     1681 cacaccgaac gaagccaccg ggccagagga aatcgaagca gatgccggca gccaccctgg
     1741 cttcctcaag gtcgaggtgc tcccagacgt gctcggcgag tatccggctg aggctgctgg
     1801 gccgaaaccg ttcctcccag tcggatcgcc tcgtcagatc gagttcctct tcttgggtcc
     1861 ggatactcct gtgaggtagc accgacaatc acgcgcattt catcgtcgtt catcattgcg
     1921 tgcagctctc cgcattcctc gtcatcctgc cccccacaca ggatcccgcg gcaaatccaa
     1981 caccagcggc atgctctcca ggcaggtgcc accatcccta cgacggagcg accgtttatc
     2041 ccccaatgag gcgagactaa ggcgcgccgc ttatcttctt ccccaccatg agagcaagga
     2101 acgcagattc tccgccaaat tgagcgtcgt ccgcaacaac atcaggacaa tatcactggc
     2161 agggtctgga gaccgcgcag gacgagcccc gctcgccatt ccggttgcgt cgccccaagt
     2221 cgcaggtcgg gggcctcctg ggtgagcgtc tggaaggcga tctccccttc catcctggca
     2281 agtggggatc cgagacagaa gtggatcccc atgccgaacg acataatccg tccgacttcg
     2341 cggccgatat cgagcctgtc cgccgcctcg aacgccctcg gatcccggtt ggccgacccg
     2401 agcacaaatt ggacctctgt cccttgccga acaggcgttc cggcaatctc caggtcctcc
     2461 attgccgtgc gtgtcgtcat ctgcaccggg ctgtcgtacc gcagcaattc ctcggtggca
     2521 ttccgcgcca gtgacggatc acccacgaga gcctgccact gatcgggatg ttgcaggaga
     2581 gcataggtcc cgttccctat caggttgacc gtcgtctcgt gccctgccac cagcagcagg
     2641 atgagcgttg agatgagttc cttctcgctg atcttccctt gcctggcctc cgccgcgata
     2701 agcgaggaga tcaggtcatc cttcgactcc ttgcgccgct cggcgacaat ccatccgaga
     2761 tactcgacga gttcgctggt cgtctgatca gcgcgttgga cgaactcctg aagaccggtc
     2821 accggcagat tgatcgcggc ggcgatgacc gcggaccaat cccggaatcg cgaaaaatca
     2881 ctctcgggaa caccgaggat gccggcaatc accaggacgg gcagcgggta ggcaaaactt
     2941 gcgatgaact caacgggccc atcctgcctc ttcatgtcct caaccagatc gagcgcaagt
     3001 cgctcgattg gggcacgcat cttgttgaca tgtcgtgggg tgaacgccat gttcgccgtc
     3061 gagcggagcc tcgtatgatc gggcggatcg cgaaagagca tgaaatggtc ggcgacatca
     3121 cgaaaggaat tcacctcagg ttcaggtggc tgttctcccg gaggcaacaa gcgacgccat
     3181 tccctcccaa gccggggatc cctcagccac gcctgcacat cttccatgcg tagcagcacc
     3241 atggagggat ttgttaagcc agctgccctc gccccaggcg actcgccctg cctcccggaa
     3301 ccgctagtag tacgcataga tttcttccgg ccgtgcatcc cggtgaggcg cgaacacgct
     3361 gcttgcttcc atggaaacgt ccccatgtat catggataga ccaccattcg ttcattcctc
     3421 cggagtgata tcggaaatgt ccggtcccgg tatgtcgcgg tatacacgga agtgtacgca
     3481 ggcatacccg caacggtcat ccgagtggag ccaggaaaga tgtcccctct ctcatcacaa
     3541 caaccaaccg ggtttcgcga tcgcgtcgat gccggtgcgc ggcttgccat gccactcctc
     3601 ccaagggttg ccaccctggc gccggacgac atcgtggtta tcgggctcgc ccgcggcgga
     3661 gtgatcgtcg cagctgaggt cgcacgacag ttggacgtcc ggctggaagc gatcgtggtg
     3721 cgaaagctgg gcgcgccgga ccagcgcgag ctggccatcg gcgccctcgc ggcgagcggc
     3781 gagcgcgtgc tcaaccagcc cctcatcaac gacattggac tggatcagga acaggttgag
     3841 tcgatctcca gccaggcaca gaaggccgca atgtcgctct gccgggagat cggtgtgccc
     3901 gcggtggtcc ccgatattgt aggcaagacc gcgattctcg tggatgacgg cctcgcaact
     3961 ggcgcgacca tgcgagtggc gatcgaggcg gtctaccacc agggagcacg ccaggtcttt
     4021 gtcgccctcc ccgtggcccc ggcgtccatg gtagagccgt tcagggaaat cgccgacgat
     4081 gtcatcacga tcattgcccc ggagaatctt cgggccgtgg gacagtggta cggttacttc
     4141 cccgacgtcc cctcctctgc ggtccgcgat gcgctagcag cgaaccgggc gagcgcctga
     4201 cagagggccg gattgcgtga gcgttgctgg ac
//
LOCUS       DASYWM010000185        20133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_345420, whole genome shotgun sequence.
ACCESSION   DASYWM010000185 DASYWM010000000
VERSION     DASYWM010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 20133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20133
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345420"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1406)
                     /locus_tag="VGR29_07660"
     CDS             complement(18..1406)
                     /locus_tag="VGR29_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2073504.1"
                     /translation="MSNERGRITGNRLVTRRDALRGTAGIAGAATVGAALSRGVTAAP
                     RTNREYSSRAQNGEIIFLSSQLTPIEEAEKMRNSILSGFDGSVEFVPEDPGPFVDRVT
                     AEAQSGSGSVGVVGGLHGDFAQFVEQDLLEDLTDLADQLADRGFIEDYLELGRYGTES
                     VYYIPWMQATYIMCAHRDALEYLPEGLTEETLSTDLTYEQLTQWGESLNENIGPVLGF
                     PAGEDGLIHRFFQGYAYPSFTGGLNTTFSGPEAVAMWEWFRSAWAVTNPQALTYNNMD
                     VPLQSGEVLVAWDHTARLINALRETPDDFVTFPAPRGPEGLGYLPAIAGLAIPKSAPD
                     KESSRALIDYLTMPETQLTTLQEVAFFPVVDVELPEDLEAGIQAEADAIQATTSAENA
                     ITSLLPVGLGEQNGTYNKVFRDTFTAIAVDENDIQPVLDEQSQVLQEVLNTAEAECWA
                     PDPESDGVCQVG"
     gene            complement(1660..2604)
                     /locus_tag="VGR29_07665"
     CDS             complement(1660..2604)
                     /locus_tag="VGR29_07665"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEV2073505.1"
                     /translation="MWWIFLAVLGVSAALIKMFDRKVPGSLYKIVLLIWAIGPLVGTL
                     WAMATLWRDWITLEEFTLFLVLYFLTGMGITAGFHRLLTHSSFETPAPVRATFLILGC
                     MAAQGRPIDWASNHLKHHAFSDHEGDPHTPTEGLFHAHLGWFFTGEPADREKYGKRWE
                     NDRVIQFIDRTTVVWLVLGLVIPYLIAGWGGFLWAGLVRVAFLNHATFAVNSICHAFG
                     SRDFETKDESRNNWLVAALTFGEGWHNNHHAFPAMAYHGMSWRQFDATAVVIKFLEKV
                     KLAKNVKLPRPELVERRRGADKEVLAPATVAVAREMGD"
     gene            3012..7364
                     /locus_tag="VGR29_07670"
     CDS             3012..7364
                     /locus_tag="VGR29_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEV2073506.1"
                     /translation="MASFDSGSVFAQDKESTSPRPVSPTDISQFIRLDQCQRFLRLQL
                     HTRSRSDDFMRDYDVTLQAIPPILTRSGARFEELIEQDVIGRFPATRFGDEEGKATGA
                     PHHNAAVIARALALAPGEAHVLFQPRLRAEVSDWSIRGVMDILRLERDQDGLLHCLIA
                     DMKASTASKVEHRLQVAIYHEMLAAIFNQARIEHAPLELAILYRGPAEGTDAADRVDP
                     ELQHRQREDAIATFGTGHGYLQRIEDPGPYVGSVQDLLTGPESTARRVLKADFEAVPF
                     HLTYKCDGCLYNEFCMKRSAETDDLSLLPHLSDQDKSILRKYGISTVRQVAGLKELVS
                     PGDNGSYPQLIPAAGKEDLCRRLSVTWPVGQHLDELIHRAQRYLYWKKEGPPTLSYIP
                     NKGYGTLPYSGPDLHPNLVRVYIDAQHDYLNDRIYMVGARVVACNNGVEEMHRQRNIV
                     RITDVPPDSNAIEEQLFVDWITETLRAIVELAAPDAGGNHAAPIHLVFINRFAQQQLL
                     NGLGRYLTKILGATALYDFMTQMAIYDSPIATFLDQEIQEQKNYPMVCQSLQAVAAYL
                     KFDWNKDVPYREIFRTRMFDFWRKFDETIGEEQQVPGINGWYTSRARFNSQIPLEYAY
                     TAWGDVSAETTLEALSDYGEATTERMLGFQARRLEAMEHIAKDFRGNRQTTLTSFVLP
                     DLAAFEQCAPTLAHALDEFVSIERFVNLAAWKHQRLAPPEQRVLNGESLVVRYVEADQ
                     EPGIAQQNRENQVREELRQRYRAEWRAVNPNGKQIRLPKDQRAESDWNHVGTRFRLRI
                     ETEDLACGLDEALNLSTLKPGSRLVLYDRWSLDTRLPENERAPFTSTSKQILYGPRVE
                     LIQIKVERDHLDRALEAFAMVEILGGGGGDKRGFLFGSGSSDPLVPDRLYTLDPDPNN
                     INGFWAAKVTEGLIAGNHNALYRLLTGDPAPPSTPCPEAAAAQQRLMDGLVALHHAGQ
                     MVDFEPAKHAFISQHGDDPVLLVQGPPGTGKSFSTAYALLARIQGRMAVGMPCRVILS
                     CKTHAAIDVLLQNILEAREQLRGLFDTQPELMNQFFDRRLCDVPLFRQRPRADVAEGI
                     TPLSNEMKAKEVIASISAPQWSVTASTPGGIYSMVKDDLFGQAFIDYLVLDEASQMSI
                     PEAIMAALPLKEDGRIIVVGDHRQMPPIVSHDWSNETRRSFREFRSYESLFLALLALD
                     PPKINFERSFRLHADLAEFLRKEIYHRDGINYHSKRHDVLPVFPLPDSFIQAVLAPQH
                     PLTVVVHDEAESQHRNSYEQELIAPILRVLADEQRYNLSPEEGLGVVVPHRAQRAALQ
                     EGVPELIRIDPETNAVTVSAVDTVERFQGDERTVILIGSTESDREYLLATGNFLLDPR
                     RLTVALSRAKKKLVLVASRSVFEIFSADEEMFQNAQLWKNLLRGTCTVKLWEGDRHGH
                     HVEVWGNVPSEPQA"
     gene            complement(7549..8748)
                     /locus_tag="VGR29_07675"
     CDS             complement(7549..8748)
                     /locus_tag="VGR29_07675"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073507.1"
                     /translation="MSAGIEERDAATPAMGSTLTPWQLRSGYFWYYAAIGAFAPFIPL
                     YYQDLGMSGLQLGVLLALPAFWTALTGALWGAIADSLAIHRMLLRIVLLVAAVVAILV
                     SMPGRFITLLPLIAILALAIVPIPPLLDGYAVSVAERSKSSFGGLRVWGSIGFTAMVL
                     VLGRLLGGQLTDRFLYAYALCLLLTWLSIFGLPRLAERRPRPLLDGLQAVRRSRPFLL
                     LLFVAYLMSSGFAVINNLLSIHVRDMGGSTQMIGLVFAVASVSEFPILIFSGWLMTRI
                     GARGMVTVALAMWVVRFAILSFVSAPWLVVGAQSLHGVTFGAFLIASVTLAHRFVGQE
                     NAATAQAALATMSWGFGSITGAIAAGSLLDVAGTYTIFRGVTVLMVVTLAVFIVGNRV
                     ISIEGNG"
     gene            8955..9467
                     /locus_tag="VGR29_07680"
     CDS             8955..9467
                     /locus_tag="VGR29_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073508.1"
                     /translation="MSDDHSPGDARKPYSDRGGPEGRHAETHDVRREQLTNPQGNRAT
                     ADSDRPTPQEFAEDIAPQTPQRVHQEHLEADSAADDKRVGNMLPELSDDELSRLPILD
                     PGTPLEQGGVYFNLKDREKGPFKAIGGQEVGAKDKMIAKKMTDYELWNQIVGRDDTAE
                     IERPSQEQSR"
     gene            9478..9810
                     /locus_tag="VGR29_07685"
     CDS             9478..9810
                     /locus_tag="VGR29_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073509.1"
                     /translation="MSDSLSPSNRNPLPEQETEQETPQDEPESSDGVRHETLGNPEEG
                     LKPMEESFARDAGPVTPERAHGDQVVAEGASDDQEVGNPPLELSDKEDPWNRQTGATV
                     ISETQTPG"
     gene            complement(9904..10503)
                     /locus_tag="VGR29_07690"
     CDS             complement(9904..10503)
                     /locus_tag="VGR29_07690"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV2073510.1"
                     /translation="MVRLGHKEEDRRTARDAGVLLLRLTAGGLMAGHGAQKLFGWFGG
                     YGLEGTSGWLESMGMKPGRPWAIAAGGSEFGGGLLTALGLMHPLGPISMLGSMAVAAR
                     KVHWGKPIWVTEGGAELPVLNIAVGVALSMVDPGRFSADRAVGLRVPISLSMIAFAAV
                     VLGVLASEATPAPVFEEAADEAGNELQGESEETLLEETM"
     gene            complement(10630..11397)
                     /locus_tag="VGR29_07695"
     CDS             complement(10630..11397)
                     /locus_tag="VGR29_07695"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV2073511.1"
                     /translation="MRLEGKTALITGADSGIGRATALTFAREGADVAIHAFGDHRGAV
                     QAVEAIREHGRRVELFEADFADPSSAEGLVAQVVERMGKLDILVNNAGKGEGKDSALD
                     VDTKSFVEVLNVDLVAPFILAREAARHMAERGTGSIVNITSVHEEIPNEGSPAYCAAK
                     GGLRMVTRTMALELAPKGVRLNNIGPGMIKTPMTVSSLFDAGTTDQAEAKIPMGRAGE
                     QQEIANLALFLASDESSYVTGSSYFADGGLMLSIQGT"
     gene            complement(11506..11736)
                     /locus_tag="VGR29_07700"
     CDS             complement(11506..11736)
                     /locus_tag="VGR29_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073512.1"
                     /translation="MSQAKKQSESATGVSNPAYDAMTVLTNKLQSIAVLEQYKQDAQG
                     DQELLQCFEQIQERERADVDQLRGLVAQRLQQ"
     gene            complement(11825..12310)
                     /locus_tag="VGR29_07705"
     CDS             complement(11825..12310)
                     /locus_tag="VGR29_07705"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEV2073513.1"
                     /translation="MAHNEFLVAWLKDAYAMEKGIAESLERQVELAKDHPTVQSGIQR
                     HLDATNGHAETVKECLESLGESPSGVKSAMASVGGMVQGMMPGAAKDDLLKAAMQDYS
                     AEHMEIASYRSLILSATELGHADIVQKCQTILEDEQAMALWLEESMPMLTREAILQGQ
                     T"
     gene            12498..13760
                     /locus_tag="VGR29_07710"
     CDS             12498..13760
                     /locus_tag="VGR29_07710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073514.1"
                     /translation="MIGSAQEQRARAAVASARLGIQTPAGRGFALISGATVAVGFSMA
                     AQQNIVSNYFENSLGLSGSQFGYITAIREIPGFLLIFLTALFYRLSLPHLTAGALALL
                     AIGYGFFGVSWSFWSVTPWVIISSMGYHTWLQTQAALGMSLTTENKSGGILGRLAAIN
                     QGGALVAMTFVLIVFHYDWLDYKQTFIVCGALALLAALFIARFPHMHNGELQERAARR
                     DRMVLRREYRLYYLLSLLDGARQQIFFSFGLWVLVDHFSLDVPAISAIMLVTAAIAMM
                     AGPWIGRQIDAHGERTMLGIVNVAYIVALLGYALIDQVGVAVGCYVVYSFIFPLSAMG
                     ASVYLRKIAPSNDVAPSLAMGLTMQHAAAVIVPILTGYVLNYVGYQIPFLVASVFAAI
                     TVIITRQLDPANQKSPRRLQEDAARASA"
     gene            complement(13817..14671)
                     /locus_tag="VGR29_07715"
     CDS             complement(13817..14671)
                     /locus_tag="VGR29_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2073515.1"
                     /translation="MKKVRNIVVTGSSGKAGQAVTKHLAANGYAVTATDVAPSPNDLG
                     VPFIRADLTDYGQAVDVLHGADAVVHLANIPAPGIYTPSTTLNANITMNGNVFLAATQ
                     LGLSRVVWASSETTLGLPFDPPRYAPVDEDHFPYPASTYALSKVLSETMGEHISGWSG
                     IPFVGLRFSNIFRPEEYVRVPGYWANPHDRKWNLWGYIDVRDVAASCRNALEADISGA
                     HNVIIAAADTIMNRPSAELLQEVFPEVELRREVGEFETLLAIGRARELIGFSPQHSWR
                     AHVSENRS"
     gene            14862..15689
                     /locus_tag="VGR29_07720"
     CDS             14862..15689
                     /locus_tag="VGR29_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02587 family membrane protein"
                     /protein_id="HEV2073516.1"
                     /translation="MGAWAQERDDFIRAFSGAYIFGIPLLFTMEMWWIGQYADLWKLL
                     VFLGLAFAANLGLTYAAGFKRRSTFGTTVNQAVDAVAVGIVAATIMLLLLNQIRLTEP
                     LDSIVGKIVVQAVPLSIGASVANQIFGKRGEKSRQGESHGEPMGPWHSFFSDVGATII
                     GGIFIGFSIAPTDEVPLIASALDYHHLVAVIGFSLLVSYMIVFASGFDQASPQGLFQR
                     PFTETMLAYLVSLLVSFLALYLFDQIEMGDPVLSMLEKTLVLGVPATVGGAAGRLVI"
     gene            15686..16147
                     /locus_tag="VGR29_07725"
     CDS             15686..16147
                     /locus_tag="VGR29_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02588 family protein"
                     /protein_id="HEV2073517.1"
                     /translation="MNQKQTHDQAQEERNGSSSNQTSPSGDKDRSVAEWTTLGISIAI
                     LVFILGLTTYLYVRGGEKPAAILVEPQMQELRQDNGGYYLPVIVRNEGDPTVEEVNIE
                     AELDTGAGEPETAQFTVNFLAGGEQVEGTFVFQHDPAEGDLTTRVMSFQEP"
     gene            16389..17282
                     /locus_tag="VGR29_07730"
     CDS             16389..17282
                     /locus_tag="VGR29_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HEV2073518.1"
                     /translation="MAIKTVGLLSPGDMGHSIGHVLHSNGLRVLTCLEGRSERSRELA
                     GRAGIEDGGSLEDVVKEADLFISVLVPSSALSVAKSVATALHASGSTVVYADCNAVAP
                     NTVRGVADLITEAGSSVADVGIIGPPPRKPGTKFYVSGPGATRFSELGSHGLDIRVLD
                     GDIGQASALKMCYGALTKGLQALATELLVAARLAGIEEALREEQTETMGEVLGWLEGN
                     MPIMPPKAYRWVGEMEEIGSFFGSLGMTPDILNGAADVYRFVAETPIGKESPETRDPN
                     RELDGVIAALAEALSDRNNAS"
     gene            complement(17305..17760)
                     /locus_tag="VGR29_07735"
     CDS             complement(17305..17760)
                     /locus_tag="VGR29_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2073519.1"
                     /translation="MQPTIETVTDLFRSVHQQLREIVKDMDSEQLNWSPAPEANSVAV
                     LVTHTLASELDTLLLVRDLAGERDRDAEFRTVAPGASDLLVAIDRADELLTQHGESIT
                     GDELMAIRTRPNRNPQIALHWLINNYGHAREHLGHLQLTTQMYQQSTKR"
     gene            complement(17789..19462)
                     /locus_tag="VGR29_07740"
     CDS             complement(17789..19462)
                     /locus_tag="VGR29_07740"
                     /inference="COORDINATES: protein motif:HMM:NF006052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV2073520.1"
                     /translation="MMSERIRAGVAATRILEQRGVQFAFGVPGESFLGLLDALYETPN
                     IQLIGTRHEGGAAFMAEAVGKLTGIPGICMGTRGVGTANLTIGIHTAFQDSTPLLALV
                     GQVETFIRHREALQEVELAPFLSHITKWAVEVPTGERLPDIVSEAFRRSISGRPGPVA
                     LALRGDILDEEVSSELPPPSDPPTAGPDSDKVKSALALLARAERPLILAGGGVLRSRA
                     TADLVAFAERTGIPVVTAFRRHDAFPNDHSLSIGSLSIGAPAAVIERARNADVVLAIG
                     TRLGETTTFAYTVPGPNAVLIHVDVSPEVVGRTFPATIGIAADAAKTIQLFTREAANH
                     QWPDRSAANRHDREVFERETAIPATSSVRDLVDPALVIGALQKRLPDDAVITCDAGNF
                     YGWLSRYFRYRQPGTFLGPTSGAMGYAVPAAVAAALVRKNRVPSVAVAGDGGFLMTAN
                     ELAVAAQHGLNVKCVVFNNSMYGTIRLHQERAYPGRVAGTNLASPDFVRLAEAFGGMG
                     VRIERNTEIADGVRQVFEHPGIGILEVAVAEDTIAVGQSLENLRTAARP"
     gene            19621..>20133
                     /locus_tag="VGR29_07745"
     CDS             19621..>20133
                     /locus_tag="VGR29_07745"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV2073521.1"
                     /translation="MAEQIGLIGLGIMGKPMARNLVKAGYSLVVHNRSRGAVEELTSE
                     SDAITAAGSPREVAEKVSIVITILPDSPDVSAVVFGENGILEAMNGGSLLIDMSTIAP
                     ATAIQVNEALVSRGASALDAPVSGGDKGAIAGTLSIMVGGEKADFDRAMPFFQAMGKT
                     IVHVGASGAGQ"
BASE COUNT         4107 a         6318 c         5805 g         3903 t
ORIGIN      
        1 ctctctgcag cacggaacta tccgacctgg cagacgccat cgctctccgg gtcaggagcc
       61 caacactcag cctcagctgt gttgagaacc tcctgcagca cctgcgactg ctcgtccagt
      121 accggctgga tatcgttctc gtcgaccgcg atcgccgtaa acgtatcccg gaagaccttg
      181 ttgtaggttc cgttctgctc gcccagcccc accggcaaga gcgaggtaat tgcgttctcg
      241 gcgcttgtcg ttgcctgaat ggcgtcggct tcagcctgga tgccggcctc cagatcctcc
      301 ggaagttcca cgtcgacgac cgggaagaag gccacttcct gcagcgtcgt gagctgtgtc
      361 tcaggcatcg tgaggtaatc gatcagcgcc cggctgctct ccttgtcggg agcgctcttc
      421 gggatcgcca accccgcgat ggcaggcagg tagcccagtc cttccggacc gcgcggtgcc
      481 gggaaggtca cgaagtcgtc cggcgtctcc cggagcgcat tgatgaggcg agccgtgtga
      541 tcccacgcga ccaggacctc tccggattgc agcggcacat ccatattgtt gtaggtcagg
      601 gcctgaggat tggtcacagc ccatgcacta cggaaccact cccacatggc cacggcttcc
      661 ggaccgctga acgtggtgtt cagcccgccg gtgaagctcg gatacgcgta accctggaag
      721 aatcgatgga tcaaaccatc ttcgccggcc ggaaaaccga gaaccgggcc tatattctca
      781 ttcaggctct caccccactg ggtcagctgc tcgtaggtca ggtcggtcga cagcgtttct
      841 tctgtaaggc cctctggcag gtactcaagt gcatcccggt gcgcgcacat gatgtacgtc
      901 gcctgcatcc aggggatgta atagacactc tccgtgccat agcgtccgag ctccaggtaa
      961 tcctcaatga agccccggtc ggccagctga tccgccaggt cggtcaaatc ctccagcagg
     1021 tcctgctcga cgaactgggc aaaatcaccg tgaagtccac caacaacgcc aaccgagcca
     1081 cttcccgact gcgcctcagc tgtcacgcgg tcaacgaacg gacctggatc ctccggaacg
     1141 aactccacgc tcccgtcgaa tccgctgaga atcgagttcc gcatcttctc agcttcttca
     1201 attggtgtca gctgggacga aaggaagatg atctccccgt tttgtgcacg ggaactatat
     1261 tcccgattgg ttcgaggagc cgccgtcacc cctcgggata aagctgctcc cactgtggcc
     1321 gctccggcga tccctgcggt accgcgaagc gcgtctcgtc gggtcaccag acgattgcca
     1381 gtaatcctgc cgcgttcgtt cgacatgggt gcctcctctt cagatccgcg atcaatgcca
     1441 gccattgacc ggtcaaccga gtgttggaac cgatggatac gattgtgtgc ttaggttcga
     1501 tggtatcaca ttgagaaatg caggctttca cgccggctga atggtgacgc gcccgtttta
     1561 ccgtcaccat tcgcctgctt gccctaaggg tcttggacgc aaaaaggggc gggcaaaatg
     1621 cccgcccctt tccgtgtgtt cagagatcct gcgatccggt tagtcgccca tttcgcgggc
     1681 aacggccacg gttgcgggag caagcacttc cttgtcagct ccgcggcgac gctccaccag
     1741 ctccgggcgg ggtagcttca cgttcttcgc gagcttgacc ttctccagga acttgatgac
     1801 gacggccgtc gcatcgaact ggcgccagct cataccgtga tacgccatgg cagggaaggc
     1861 gtgatgattg ttgtgccaac cctcaccgaa cgtcagcgcg gcaaccagcc agttgttgcg
     1921 gctctcgtcc ttcgtctcga agtcgcggga gccaaacgca tggcagatcg agttcactgc
     1981 gaatgtcgcg tgattgagga aggcaacgcg gacgagaccg gcccagagga aaccacccca
     2041 gccggcgatc aggtagggaa tgaccagacc aaggaccagc cagacaaccg tggtgcggtc
     2101 gatgaactgg ataacccgat cgttctccca gcgcttgcca tacttctcgc gatcggcggg
     2161 ctctcccgtg aagaaccagc caagatgcgc gtgaaagagt ccctcagtcg gggtatgtgg
     2221 atctccctcg tgatcggaaa aggcatgatg cttgagatga ttcgacgccc aatcgatcgg
     2281 acgaccctgg gccgccatac atccaagaat caggaacgtg gcgcgaaccg gtgccggtgt
     2341 ctcgaagcta ctatgcgtca acaaccgatg gaacccggcg gtgatgccca taccggtcag
     2401 gaaatacagg acaaggaaga gtgtgaactc ctcgagcgtg atccagtccc gccacagcgt
     2461 cgccatggcc cacagcgtgc ccaccagagg gccgatcgcc caaatcaaca ggacgatctt
     2521 gtagagcgag ccgggcacct tgcgatcaaa catcttgatc agtgccgcgg acacaccaag
     2581 gaccgccaaa aaaatccacc acatggtccc gaaaactccc ggatgccctt ggacatccct
     2641 tgataacgtc agcccggata caagtggaca cgccaggagc gggataccat tcctgatcac
     2701 aagctgccca agggtttgtg actgactcac gaattgtacg cgggatggac ccgatccgtg
     2761 taacgttctc ccggcaatgc tcgcactttg cggtgagcat gcgcgcggtg aacctatgaa
     2821 gtccgccctg atgcgaaatc acgggtcatc actctgcaag atttcggcct cgccgctggc
     2881 ccgacccacc tcctggtggc cagcaccggg gactatctgc agtgccgatg gagcgccccg
     2941 cgatgtatcc ccgaagggtt acactttccc gtaaacacgg tcagaaacag gcagcgagat
     3001 gagggccagg catggcgagc tttgactccg gatcggtttt cgcgcaggac aaggaatcga
     3061 catctccaag gcccgtcagc cccaccgata tttcacagtt catccgcctc gaccagtgcc
     3121 agcggttcct ccgcctgcaa ctgcatacgc gctcacggag tgacgacttc atgcgcgact
     3181 acgacgttac cctgcaggcg attccaccga ttctcacccg atccggtgct cggtttgaag
     3241 aactgattga gcaggatgtg atcggacgct tccctgctac ccgctttggt gatgaagagg
     3301 gcaaggcgac tggagcacct caccacaacg ctgccgtcat cgcccgcgcg ctcgcgctgg
     3361 ctcccggcga agctcatgtc ctcttccagc cacgtcttcg tgcggaggta agcgactgga
     3421 gcatccgagg tgtgatggat attctccgtc tcgagcggga tcaggacggt ctgcttcact
     3481 gcctgatcgc cgacatgaag gcgagcacgg cttccaaagt agagcaccgg cttcaggtcg
     3541 ccatctacca tgagatgctc gccgccatct tcaatcaggc ccgcatcgag cacgcgccgc
     3601 tcgaactggc gatcctctat cgcggcccgg ctgagggaac cgatgcggct gatcgagttg
     3661 atccagaact gcaacaccgg cagcgcgagg acgctatcgc gaccttcggc accgggcacg
     3721 ggtatctcca gcgtattgag gatcctggcc cctacgtcgg atcggtccag gatctgctga
     3781 ccgggcccga atcaaccgct cgtcgtgtcc tcaaggcaga tttcgaggcg gtccccttcc
     3841 atctcaccta taaatgcgat ggctgtctct acaacgagtt ctgcatgaaa cgcagcgcgg
     3901 agacagacga cctctccctc ctgccccacc tttccgacca ggacaagagc atcctgcgca
     3961 agtacggcat ttcgaccgta cgccaggtag cagggctcaa ggaactcgtg tcgcccggag
     4021 acaacggctc ctatccgcag ttgattcccg ctgctggcaa ggaggatctg tgtcggcgcc
     4081 tatcggtcac atggcccgtc gggcagcatc tcgatgaatt gatccaccgg gcacaacgct
     4141 acctctattg gaagaaagag gggccaccaa ccctttcata tatcccgaac aagggctatg
     4201 gcacgcttcc ctacagtggg ccggatcttc atcccaatct cgtgcgtgtc tatatcgatg
     4261 cgcagcatga ctacctgaac gaccggatct acatggttgg tgcgcgcgta gtagcctgca
     4321 acaacggcgt tgaggaaatg catcgtcaac gaaacatcgt gcggatcacc gacgtcccgc
     4381 ccgactcgaa cgcgatcgag gagcagctct tcgttgactg gatcaccgag acgctgcggg
     4441 caatcgttga gcttgccgca cccgatgcgg ggggcaacca cgcagcgccg atccatcttg
     4501 tgtttatcaa tcgcttcgct caacagcagc tgctcaacgg tcttggtcgt tacctcacga
     4561 agatcctcgg cgcgactgcc ctgtatgact tcatgacgca gatggctata tacgactccc
     4621 ccatcgccac attcctcgac caggagatcc aggaacagaa gaactatccg atggtctgcc
     4681 agtcgctgca ggcggtcgcc gcctacctga agttcgactg gaacaaggac gttccctatc
     4741 gtgagatctt caggacgcgg atgtttgatt tctggcggaa gttcgacgaa accattggcg
     4801 aagaacagca agttccgggg atcaacggct ggtacaccag ccgcgcccgc ttcaacagcc
     4861 agatccccct ggaatacgcc tatacagcgt ggggagacgt ctccgcggaa accaccctgg
     4921 aggccctctc cgactacggt gaggcgacca ccgagcgcat gctcggcttc caggcccgcc
     4981 ggctggaggc gatggagcat atcgcgaagg acttccgggg caaccgccag acgacgctga
     5041 catcttttgt cctccccgat ctcgcagcct tcgagcagtg tgcaccgacg ctggcccatg
     5101 cccttgatga attcgtcagc atcgagcgct ttgttaatct cgccgcctgg aagcatcagc
     5161 ggctcgcgcc gcctgagcag cgcgtcctca acggcgaatc gttggttgtc cgctatgtcg
     5221 aagccgacca ggagccgggc atcgctcagc agaaccggga aaatcaggtg cgggaggaac
     5281 tccggcagcg ttatcgcgca gagtggcggg ccgtgaatcc caatgggaag cagatccgcc
     5341 ttccaaagga ccagagggcc gagtcggact ggaatcatgt cgggactcgt tttcgcctgc
     5401 gtatcgagac agaggacctg gcttgcggtc tggacgaggc actgaacctc tcgaccctca
     5461 agcctggttc ccgccttgtg ctctatgatc gctggtcctt ggatacacgc cttccagaga
     5521 atgagcgtgc accctttaca tcgacttcga agcagatcct gtatggtcca cgggtggagc
     5581 tgatccagat caaagtggag cgtgaccatc tcgatcgtgc cctcgaagca ttcgcgatgg
     5641 tggagatcct cggcggaggc ggaggtgaca agcggggatt cctcttcgga tcgggctcaa
     5701 gcgatccgct ggtgcccgat cgtctctaca cgctggatcc cgacccaaac aacatcaacg
     5761 gtttctgggc ggccaaggtg acagaaggcc tgattgccgg gaaccacaac gcgctttacc
     5821 gccttctgac cggagatccc gcacctccat cgacgccctg tccggaggcg gctgcagccc
     5881 agcagcgatt gatggacggc ctcgtagccc tgcaccatgc cgggcagatg gtggatttcg
     5941 aaccggccaa acacgccttc atcagccagc atggcgatga tccggtcctc cttgtgcagg
     6001 ggcctcccgg caccggcaag agtttctcca ctgcctatgc tttacttgcg cgcatccagg
     6061 gtcggatggc cgtggggatg ccttgtcggg taattcttag ctgcaagacg cacgcggcga
     6121 tagatgtcct gctccagaac atcctggagg cacgggagca gctccgcggc ctcttcgaca
     6181 cccagccgga gctgatgaat cagtttttcg atcgccgcct ctgcgacgtg ccgctctttc
     6241 gccagcggcc ccgcgctgat gtcgctgagg ggatcactcc actctccaac gagatgaagg
     6301 cgaaggaagt catcgcctcg atctctgctc cccagtggtc ggttactgcc tccactcccg
     6361 gcggcatcta ctcgatggtt aaggatgatc tattcggtca ggcctttatc gactacctgg
     6421 tgctcgacga ggcgtcacaa atgagtatcc ccgaagccat catggcggca ctacctctta
     6481 aggaagacgg tcgcatcatc gttgtgggag accaccggca gatgccaccg atcgtcagtc
     6541 acgactggtc taacgagacg cgtcgctcat tccgggagtt ccgttcctac gagtcgctct
     6601 tccttgccct gctggcgctc gatccgccga agatcaactt cgagcgcagc ttccgactcc
     6661 atgccgacct cgcggagttc ctgcgtaagg agatctatca ccgggacgga atcaactatc
     6721 actcaaagcg gcacgacgta ttgcccgttt ttcccctccc tgactccttt atccaggctg
     6781 tcctcgcgcc gcagcacccg ttgacggttg tcgtccacga tgaggctgag agccagcacc
     6841 gcaacagcta cgagcaagag ttgatcgcgc cgatcctgcg cgtgcttgcc gatgagcagc
     6901 gatacaacct gtcgcctgag gagggtctcg gggtggttgt gccccatcgc gcccagcgcg
     6961 cggccctgca ggagggtgtg ccagagctca ttcggataga tcctgagacg aatgcggtga
     7021 ccgtctccgc ggtcgacact gtggaacggt tccagggcga tgagcggaca gtgatcctga
     7081 ttggctccac ggagagtgac cgggagtacc tccttgcgac cggcaacttc ctgctcgatc
     7141 cgcgtcgact taccgtggcc cttagcaggg ccaagaagaa attggtcctg gttgcctccc
     7201 gctcggtctt cgaaatcttc agcgccgacg aggagatgtt ccagaacgcc cagctctgga
     7261 aaaacctgct ccgtggcacc tgtaccgtga agctgtggga gggcgacagg cacgggcacc
     7321 acgtggaagt ctggggcaat gttccttctg aaccgcaagc gtgaccgtgc tcccgttcag
     7381 ctcccggctt aggtgcgctc cctgtcgcat accagaagct ctccgagctg ggccaggttg
     7441 ctcacggtaa cgtcgggaag aggatcatcc atcgacaagc tcttccagag acgagcgctc
     7501 tgtagaattc cggcaggaga cctgccgggc tgatcatcgg gtgagaccct acccatttcc
     7561 ctcgatgcta atgacccggt tcccgacgat gaagaccgcg agcgtcacca ccatcaacac
     7621 cgtaacgcca cgaaagatcg tatatgtccc cgctacatca aggagcgaac cggcggcaat
     7681 tgcaccggtg atgctcccaa atccccatga catcgttgcc agcgccgcct gcgctgttgc
     7741 cgcattctcc tggcccacga accgatgggc cagcgtgacc gacgcgatca ggaacgcgcc
     7801 aaaggtgacg ccatgtaacg actgggcacc taccaccagc cacggcgcgg agacgaaact
     7861 caggattgca aagcggacca cccacattgc gagcgccacc gtgaccatgc cacgcgctcc
     7921 gattcgggtc atcaaccaac cactgaagat caggatcggg aactcgctca cggaggcgac
     7981 cgcgaagaca agaccgatca tctgcgtaga acctcccatg tcacggacat gaatactgag
     8041 gaggttattg atcactgcga agccgctcga catcaggtag gcaacaaaca gcagcaggag
     8101 aaatggacgg ctgcgccgca cagcctgcag accatccagc agtggtctcg ggcgacgctc
     8161 agcgagtcga ggcaggccga agatcgagag ccacgtcaac aggagacaga gcgcgtacgc
     8221 gtacaggaac cggtcggtca gctgcccgcc aagcaaccgg ccaagcacca gcaccatcgc
     8281 cgtaaagccg atcgagcccc aaacccgcaa tcccccgaag gaggattttg accgctcagc
     8341 gacgctcacg gcatacccat caagcaacgg tggtatcggc acgatagcca gcgcgagaat
     8401 cgcgatcaac ggcaacagtg taatgaatct gcctggcatt gacaccagaa tggcaaccac
     8461 ggccgcaacg agcaaaacga tgcggagcag catccggtga atcgccaacg agtctgcgat
     8521 ggctccccag agagcccctg taagcgctgt ccagaaggca ggcaacgcca gcagcacacc
     8581 aagctgcaga ccgctcatcc caagatcctg gtaatagagc gggatgaaag gcgcgaatgc
     8641 gccgatcgct gcgtaatacc agaaataccc ggagcgaagc tgccagggcg ttaatgtgct
     8701 ccccatggcc ggagtcgccg catcacgctc ctcgatccct gctgacatgg tcctctgctg
     8761 ccccaacggt ctccccttat cctgctctcc cggctggtac gccgggtccc cgactccttg
     8821 gcattttatc ggccaccgga gtcttcctct gtaagctcgt ggcgtggcgc atgaatgctg
     8881 gcacctcgca tgctcagaaa ccgggaagac cggccagaca tgccgatagt accggcaagg
     8941 aaaaggagta atcgatgtca gatgatcaca gtccaggaga cgcgagaaag ccctattcag
     9001 atcggggcgg ccctgaaggg cgccatgccg agacgcatga cgtccggcgc gagcaactga
     9061 cgaaccccca ggggaaccgt gcgacggccg acagcgaccg gccaacgccg caggaattcg
     9121 cggaagatat tgcgccgcag acgccacaac gggtccatca ggagcacctg gaagcagact
     9181 cggcggcgga tgacaagaga gttgggaaca tgctcccgga gctgtcggac gacgagctct
     9241 cgcgccttcc catcttggac cccggtactc cacttgaaca gggaggcgtc tacttcaacc
     9301 tcaaggaccg cgagaagggc ccgttcaagg caattggcgg ccaggaggtc ggcgccaaag
     9361 acaagatgat cgccaagaag atgactgact acgagctgtg gaaccagatc gtcggtcgcg
     9421 atgacactgc tgagatcgag cggccgtcac aggaacaatc aaggtaggga ggacaccatg
     9481 tcggacagtc ttagcccaag caaccggaat ccactacccg agcaagaaac cgagcaagaa
     9541 acgccgcaag acgagccgga gtcctcggat ggcgttcgcc atgaaacact aggaaacccg
     9601 gaagagggac tgaaaccaat ggaggaaagt ttcgccaggg atgccggacc cgtaacgccc
     9661 gaacgcgccc atggggacca ggtcgtggca gaaggcgcct cggacgacca ggaagtcggc
     9721 aacccgcccc tagagctcag cgacaaggag gatccctgga atcgtcaaac aggtgccacc
     9781 gtgatctccg agacccagac gcccggttag acaatttgca tgcgtaaaag aggagccctg
     9841 tcgatatgac agggctcctc tttttataac cgaatgccat tcgtgcagga ggatgtcccc
     9901 cacctacatg gtttcctcga gcaatgtctc ttctgactca ccttgaagct cattgcccgc
     9961 ctcatcggct gcctcctcga aaaccggtgc tggcgttgcc tcacttgcca ggacgccgag
    10021 cacgactgcc gcaaaagcga tcatcgagag gctaattggc acccggagac ccacggcgcg
    10081 atcggccgag aagcggccgg gatcaaccat cgagagtgcg acaccgaccg cgatattgag
    10141 gaccggtaac tcggcgccgc cttccgttac ccagatcggc tttccccagt gcactttcct
    10201 ggctgccacc gccatcgagc cgagcatgga tatcggacca agcggatgca tcaatcccag
    10261 cgccgtaagc aggccgcccc cgaattcgga gccacccgcc gcaatggccc agggacggcc
    10321 gggcttcatg cccatggatt ccagccatcc ggaggttcct tcgagtccat agccgccgaa
    10381 ccatccgaac aacttctggg ccccatggcc ggccatcaag cctcctgcag tcaaccgcag
    10441 caacagcacg ccggcgtcac gtgccgtcct cctgtcttcc tccttatggc ccaatctaac
    10501 cattgctttc atcccgtata cctccggagc atgaccgaaa cgacatcata aagatgtgca
    10561 gggcccatgc cactaagcag ccgggccacg acccttcgag ccggtgccag gagcccgagg
    10621 gactcgccgt catgttccct ggatcgagag catcagcccg ccgtccgcga agtaggaact
    10681 gcccgtcaca tagcttgact cgtcggacgc aaggaaaagt gccaggttgg cgatctcctg
    10741 ctgctcaccg gcacgtccca tggggatttt ggcctccgcc tggtccgtag tccccgcatc
    10801 aaacagcgac gacacggtca tcggcgtctt gatcatgccc ggaccaatgt tgtttaggcg
    10861 cacgcccttt ggcgcaagct cgagtgccat cgtcctcgtc accatccgca ggccgccctt
    10921 ggcagcacaa taggcgggag agccctcgtt cgggatctcc tcgtgcactg aggtgatatt
    10981 gacgatggat ccggtacccc gctcggccat gtgcctcgcc gcctcgcggg ccaggataaa
    11041 gggcgccacc aagtcgacat tgagcacttc gacaaaggac ttcgtatcga catcgagcgc
    11101 tgaatccttg ccctcgccct taccggcatt gttcaccagg atatcgagtt tgcccattcg
    11161 ctcgacaacc tgggccacaa ggccctcagc cgaggaagga tcggcgaaat ctgcctcgaa
    11221 gagctccacg cgtcgcccgt gctccctgat tgcctcaact gcctgcactg cgccgcgatg
    11281 gtcaccgaag gcgtggatag ccacgtcggc tccctcgcgt gcaaatgtca gcgcggtcgc
    11341 ccggccaatg ccactatcgg cgcctgtaat aagagccgtc tttccctcaa gtcgcatacg
    11401 tatcatccct tccgcggggg ccacccgcga tcttccctac acaacggggc gcccagtcgc
    11461 gactgggcgc cccgtgatgg ctgaatgaaa cccgaccgat cagggctatt gctgcagtcg
    11521 ctgggcgacg agaccgcgca actggtcaac atccgcgcgc tcgcgctcct ggatctgctc
    11581 gaagcactgc aggagctcct ggtccccttg ggcgtcctgc ttgtactgct caagcacagc
    11641 gatgctctgc agcttgttgg tcaacaccgt catggcgtca tacgccgggt tggaaacgcc
    11701 tgttgcggat tcggactgct tcttggcctg ggacatggtc tactccttta ctggttgaaa
    11761 actaattcgc gtgtcctgaa cgattcagtg accgatggcg ctctccggaa gcggagccag
    11821 tggatcaggt ctgcccctgc aggatcgctt ccctggtcag catcggcatg ctctcttcga
    11881 gccaaagcgc catcgcctgc tcatcctcga gaatggtctg gcacttctgt acgatatcgg
    11941 cgtgtccgag ctcggtggcg gagaggatca gcgaccgata ggaggcgatc tccatgtgct
    12001 cggcggaata gtcctgcatt gcagccttga gcaggtcgtc ctttgctgcg cccggcatca
    12061 tgccttggac catgccaccg acggaggcca tggcgctctt cacgccggaa ggactctcac
    12121 cgagcgattc caggcattcc ttgacagtct cggcgtgtcc gttcgtggcg tccagatggc
    12181 gctggatacc gctctggacc gtgggatggt cttttgcgag ttcaacctgt cgctccagcg
    12241 actcggcgat ccccttctcc atggcatatg cgtccttgag ccatgcaacc aaaaattcat
    12301 tgtgtgccat cgtagctata cctcctgttg aaagctccga tttccagccc ttacggacct
    12361 tgcatcaaca agggcaagat caatacctcg gctctccttc cggagaggcg gatggcggtt
    12421 ctcggacgga ggctggccgg atatactgcc tcccgttgga tacatcagcg ggcgccgtgc
    12481 ctcattcagg aaaagtgatg atcgggagtg cacaggagca acgagcgcga gccgcggtag
    12541 catccgcccg cttgggaata cagacccctg ccgggcgcgg attcgccctg atctcagggg
    12601 caaccgtggc cgtcggcttc tcaatggcag cccagcagaa tattgtctcc aattatttcg
    12661 agaacagtct cgggctctcc ggttcgcagt ttggttacat caccgcgatc cgcgagattc
    12721 ccggctttct gctcatcttc ctgaccgccc tcttctaccg gctctcgctg ccacatctca
    12781 cggctggcgc cttggcgctc ctggcgattg gatacggctt cttcggcgtg tcctggtcgt
    12841 tctggtccgt tacgccgtgg gtgatcatct cgtcgatggg atatcacact tggctccaga
    12901 cccaggctgc actcgggatg agcctcacca ctgagaacaa atcaggcggc atccttggcc
    12961 gcttggccgc catcaaccag ggtggcgccc tggtcgcgat gacgttcgtt ctgatcgtgt
    13021 tccattacga ctggctggac tacaagcaga cattcatcgt ctgtggtgcc ttggccctgc
    13081 tggcggcgct gttcatcgcc cgttttcccc acatgcataa tggcgagttg caggagcgcg
    13141 ctgccaggcg agaccggatg gtgctgcggc gagagtatcg cctctactac ctgctcagcc
    13201 tgcttgatgg tgcccgccag cagatcttct tctcctttgg cctgtgggtg ctcgtggacc
    13261 acttcagcct ggatgtcccg gctatttccg cgatcatgct ggtcacggcg gcgatcgcga
    13321 tgatggcggg tccctggatc ggtcgccaga tcgatgcaca cggggaacgg acgatgcttg
    13381 ggatcgtcaa tgttgcctac atcgtggcgc tgcttggtta tgccctcatt gatcaggtag
    13441 gcgtcgccgt cggctgctat gtcgtctact cgttcatctt cccactgtcc gcgatgggcg
    13501 cctcggtcta cctgcgcaag atcgcccctt caaacgatgt cgcaccctct cttgcaatgg
    13561 ggttgaccat gcagcatgcg gcagccgtga tcgttccaat tctgaccgga tacgtactga
    13621 actacgtcgg gtaccagatc ccgttcctgg tcgccagcgt cttcgcggca atcaccgtga
    13681 ttatcacccg gcaactcgac cctgcaaacc aaaagtcccc tcgccgcctg caggaagatg
    13741 ccgcccgcgc atcggcgtga tgcgcaggcg gcagcgatgt tttcggggca aattgtcgcg
    13801 ataccacgta cctttctcag gatctgttct cgctcacatg agcccgccag gaatgttgtg
    13861 ggctgaatcc gatcagctca cgggcccggc cgatcgccag cagtgtctcg aactccccga
    13921 cctcgcgccg gagttcgacc tccgggaaga cctcctggag tagttccgcc gatgggcggt
    13981 tcatgatcgt gtcggcggct gcgatgatga cattgtgcgc ccccgagata tctgcttcga
    14041 gtgcgtttcg acatgacgcc gcgacatccc gcacatcaat gtagccccac agattccact
    14101 ttcgatcgtg aggattcgcc cagtagcccg gaacacgcac atattcctcc ggcctgaaga
    14161 tgttggagaa tcgcaatccg acaaacggga tgcctgacca gccggagatg tgctcgccca
    14221 tcgtctcgct cagcaccttg gacaaggcat atgtcgacgc cggataggga aagtggtctt
    14281 catcgaccgg cgcatatcgc ggtggatcga atggcaatcc cagcgtggtc tcgctcgacg
    14341 cccagacgac ccggctcaag cccagctggg tggcagcaag gaagacattg ccgttcatcg
    14401 tgatgtttgc attcagtgtt gtcgacgggg tgtagattcc tggagccggg atatttgcca
    14461 gatggacgac cgcatcggct ccatgcagga catcgacggc ttgaccatag tcggtgagat
    14521 cagcacggat gaagggaact cccagatcgt ttggcgaggg cgcgacatct gttgctgtga
    14581 ctgcatagcc gttcgcagcc agatgcttcg tcaccgcctg gccggccttg ccactactac
    14641 cggtgacaac aatgttcctg actttcttca tgagcagatc tctctttcct tgtaatgctt
    14701 cggccgctta aagttagcag cgtgacacgc cttccgttag taaaatccgt gccagggacc
    14761 gatgtacggt tcaaccgcgc ccgccgcggg catgaacccc gcatgtcata atcctgtcac
    14821 gattgcttct gtcaccatgg caggatacgt ggagggttgc cttgggggct tgggcacaag
    14881 agcgcgacga cttcatccgt gccttttcgg gcgcctacat cttcggcatc ccgctcctat
    14941 ttacgatgga gatgtggtgg atcggccagt acgctgacct ctggaaactg ctcgtttttc
    15001 ttggccttgc attcgccgcc aaccttggcc tgacctacgc cgccggcttc aagcgcagga
    15061 gcacgttcgg cacaacggta aatcaggcgg tggatgcggt cgccgtggga atcgtggcgg
    15121 caacgatcat gttgcttctc ctgaatcaga tccgtctcac tgagcccctg gacagcattg
    15181 tggggaaaat cgtcgtacag gcagtaccgt tgagcattgg ggcctccgtt gctaaccaga
    15241 tattcggcaa gcgtggtgaa aagagccgcc agggggaatc gcatggtgag ccgatgggac
    15301 cgtggcactc attcttttcc gacgtcgggg caacaataat aggtggcatc ttcatcggct
    15361 tctccattgc accgacggac gaggttcccc tgatcgcgtc tgcgttggat taccaccatc
    15421 ttgttgccgt catcgggttt tcgctgctgg tgagttacat gatcgtcttc gccagcggct
    15481 ttgatcaggc ttctccccaa ggcctcttcc agcgaccctt tacggagacg atgcttgcct
    15541 accttgtctc gctgctcgtc tcgtttctgg ccctctactt gtttgaccag attgaaatgg
    15601 gggatcccgt cctctccatg ttggagaaaa ccctcgtcct cggtgtcccg gcaacagtcg
    15661 ggggcgccgc tggcaggctg gtgatatgaa tcaaaagcag acccatgacc aagcccagga
    15721 ggaaagaaac gggagcagca gcaaccagac atcaccctcg ggcgacaagg accgctctgt
    15781 cgccgagtgg acgacgctcg gcatcagcat cgccattctg gtcttcatcc tcgggctcac
    15841 gacgtactta tatgtacggg gcggtgaaaa gcccgcagcc attctcgtgg agccccaaat
    15901 gcaggagctc aggcaggata atggcgggta ctaccttccg gtcatagtcc gcaatgaggg
    15961 tgaccccacg gtggaggaag taaatatcga ggcggaactt gatactggcg ctggagaacc
    16021 ggagaccgcc cagttcacag tcaacttcct cgcgggtggt gagcaggtag agggaacctt
    16081 tgtcttccag catgatcctg cagagggcga cctgacaacc cgggtcatga gtttccagga
    16141 accgtgagcg acgcactcgg atccaccacc agggcaacaa gccccggttc agcttgacgg
    16201 gatctccatg cagactccgg ataaagacac tcgccgctgc gctccccctt gatgctcctg
    16261 gccgctcgcc ggtaatgggc tgccacagag acattcttta cggtcccgcc tcttgctctt
    16321 tccctccccg aacactacga tggctccctc acgagatgga caaatgatgt ggagaggaag
    16381 gatcgatcat ggcgatcaag actgttggcc tactgagccc aggcgatatg gggcattcca
    16441 tcgggcacgt gctgcactcg aacggactga gggtactcac gtgtctggag ggccgcagcg
    16501 agcgttcgcg tgaactggct ggccgcgcgg ggatcgaaga tggcggcagt cttgaagatg
    16561 ttgtcaagga agccgatctc ttcatcagtg tccttgtccc ctcctccgca ttgtcggtcg
    16621 ccaaatccgt ggcaactgcc ctgcatgcaa gcggatccac cgttgtctac gcggattgca
    16681 acgccgtcgc accaaacacg gtacgcggag tcgccgacct cattacggag gcaggcagct
    16741 ccgttgctga cgtcgggatc ataggtccac cacccagaaa gccaggcacg aagttctacg
    16801 tatctggccc gggcgccacc cggttcagcg aactggggtc acatggtctg gacatccgtg
    16861 tgctggacgg cgacatcggt caagcctcag ctcttaagat gtgctacggg gcgctgacga
    16921 agggtctgca ggcgttggcc accgaattac tggtcgcggc caggttggct ggcattgagg
    16981 aagcgttgcg cgaagagcaa accgagacca tgggggaagt ccttggctgg cttgaaggaa
    17041 atatgccaat catgccgccc aaggcctatc gctgggtcgg agagatggaa gagatcggtt
    17101 cattcttcgg tagtctgggc atgacgccag acatcctcaa cggtgctgcc gatgtctacc
    17161 gattcgtcgc ggaaacccct atcggcaagg aatcaccaga gacacgggac ccgaaccgcg
    17221 agctcgacgg cgtgatcgcc gcgctcgcgg aggccctcag cgacaggaac aacgcctcat
    17281 gaggatacgt ggccgcttat cagctcacct cttggtcgac tgctgataca tctgcgtcgt
    17341 cagctggaga tggcccaggt gctcacgggc atgcccgtag ttgttgatca gccagtgcag
    17401 cgcaatctgt ggattccggt tcggacgtgt ccggatcgcc atgagctcgt ccccagtgat
    17461 gctctcgcca tgttgtgtga gcaactcgtc cgctcggtcg atagccacaa gcagatcaga
    17521 agcgcccggg gccaccgtac ggaattccgc gtcgcgatcc cgctcacccg cgagatcccg
    17581 caccagcaga agtgtgtcga gctccgatgc gagcgtgtgc gtcacaagca cggccactga
    17641 gttggcttcg ggtgccggag accagttgag ctgctcgcta tccatgtctt tcacgatctc
    17701 acgaagctgc tgatgcacgg atcgaaacag gtcggtgacg gtttcaatgg tcggttgcat
    17761 ctgtgttctc ctcttgtacc cactgccgct atggacgggc agcggtccgt agattttcga
    17821 gtgattgacc cacggcaata gtgtcctcgg ccaccgcaac ctcaagaatc ccgatcccgg
    17881 gatgctcgaa tacctgccga accccgtcgg caatttctgt gttccgctcg atacggacgc
    17941 ccatacctcc gaacgcttcc gcgaggcgaa cgaaatccgg gctggcgagg ttggtgccgg
    18001 caacccgacc tggataggcc cgctcctgat ggaggcgaat cgtaccgtac atcgaattgt
    18061 tgaacaccac acacttcacg ttgagcccat gctgggccgc aaccgccagc tcgttcgccg
    18121 tcatcaggaa ccccccgtca cctgcgactg cgacggacgg aacgcggttc tttcgtacca
    18181 gggcagcggc caccgccgcc ggcacggcgt accccattgc cccggatgtt gggcccagga
    18241 acgttccggg ctgcctgtag cgaaaatacc tggagagcca accgtaaaag ttgccagcgt
    18301 cgcaggtaat caccgcatca tccgggagcc gcttctgcaa cgccccaatc accagggcag
    18361 ggtccacgag atcgcggacg gaagatgtcg caggtatcgc tgtctcgcgc tcgaacactt
    18421 cccgatcgtg cctgtttgcc gccgaccgat ccggccattg atggtttgca gcctcacgcg
    18481 tgaagagctg gatcgttttg gcggcatcgg cggcaatccc gatcgtcgcc gggaatgtcc
    18541 gccctacgac ctcaggcgag acatcgacgt ggatgagaac tgcgttgggc ccaggaacgg
    18601 tataggcgaa cgttgttgtc tcgccgagcc gcgtccctat ggcaagaact acgtcagcat
    18661 tgcgggcacg ctcgatcacg gcggcaggcg caccgatgga gagggagccg atggagagag
    18721 agtgatcgtt tgggaaggca tcatgccgcc gaaaggccgt gaccaccggg atgcctgttc
    18781 gctcggcgaa agcaacgaga tccgcagttg cccgactccg gagaaccccg cccccggcga
    18841 ggatcagggg acgctccgcg cgcgccagca gcgccaatgc cgatttcacc ttgtcactgt
    18901 cgggccctgc ggttggcggg tccgaagggg gcggaagctc cgaggaaacc tcctcgtcga
    18961 ggatatctcc cctgagtgcc agggccaccg gacctggccg cccgctgatg ctgcgtcgaa
    19021 atgcctcact gacgatatcc ggcagtcgct ccccggtggg cacttcgacg gcccatttag
    19081 tgatatggga gaggaacggt gcgagctcga cctcctgcaa cgcttcgcgg tgacgaataa
    19141 acgtctccac ttggccgacg agggcgagca agggcgtgga atcctggaaa gcagtgtgga
    19201 tgccaatcgt cagattggcg gtgccgaccc cgcgcgtgcc catgcagatc ccggggattc
    19261 cggtcagctt gccgacggcc tccgccatga aggcggcacc gccctcgtgc cgcgtgccga
    19321 taagctggat attcggcgtc tcatacaggg catcaagcag gccgagaaaa ctttctcctg
    19381 gcactccgaa ggcgaactgg acgccccgct gctccaggat cctcgttgcc gcgactcccg
    19441 cccggatccg ttccgacatc atccccttct cccaacgagt gagctgaccc gctcaacacg
    19501 acctcatgtt ccatatgcgc aattgaccat atcgaagacc ggcgcatagt atgcaggcac
    19561 aaagaaccgt cgatgtcgat catcgcgcag aaacgtaaga gaaggaagga ccccctccca
    19621 atggcagaac aaataggact tatcggtctc ggcatcatgg gcaaacccat ggcccgaaac
    19681 ctcgtcaagg ccgggtactc actcgttgtt cacaaccgct cacggggcgc cgtcgaagag
    19741 ctgaccagcg agtccgacgc gatcaccgcc gcaggcagtc cccgtgaggt tgccgagaag
    19801 gtgagcatcg tgatcacgat tctgcccgac tcccccgatg tcagtgccgt ggtctttggc
    19861 gaaaacggaa tcctcgaagc gatgaatggg ggcagtttgc tcatcgacat gagcaccatc
    19921 gcacctgcga cggcgattca ggtcaatgag gcgctcgttt cacgcggggc ctctgcgctg
    19981 gatgccccgg tgagcggcgg cgacaagggc gcgatagcgg ggacgctcag catcatggta
    20041 gggggtgaaa aagccgactt cgaccgcgcc atgccgttct tccaggcgat ggggaagacc
    20101 atcgtgcacg ttggcgcgtc gggtgccggg cag
//
LOCUS       DASYWM010000186         6660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_138987, whole genome shotgun sequence.
ACCESSION   DASYWM010000186 DASYWM010000000
VERSION     DASYWM010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138987"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VGR29_07750"
     CDS             <1..523
                     /locus_tag="VGR29_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV2073522.1"
                     /translation="VIGLRWEDVDFDQGTIAIRKQLQVINGQPTFTEPKSKMSRRTIP
                     LPPTVMDLLRQHRRQQLEERMRAGERWQAQWGLVFCRSTGTPLDNSNLRKHFKRKLEG
                     AGLPPMRFHDMRHSAASMLAARGVHPSVAQRILGHANINTTLAVYTHVEDAMMRQATD
                     LMGDLYETEMEAN"
     gene            complement(656..1093)
                     /locus_tag="VGR29_07755"
     CDS             complement(656..1093)
                     /locus_tag="VGR29_07755"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEV2073523.1"
                     /translation="MANTNTQSNNVGTLMRLSDTGLTVADPAEDVRGLKLLDRNGEEI
                     GTVDDLLVDDTENRVRFLRIAHGGFLGIGEEHFLVPVDAITRIDSEHVHIDRERSKLS
                     DAPGYSPDLAEAPDYYPGVYGWWGYSPYWSGGYTYPGYPVYPR"
     gene            1403..2035
                     /locus_tag="VGR29_07760"
     CDS             1403..2035
                     /locus_tag="VGR29_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073524.1"
                     /translation="MGKRVFAPVALVAAAFIFMMSFVLGGATSAQGTATPADGGVEVD
                     PSHPAHIHSGTCEELGDVVFPLNNVTPLGVDVSPATPVDMTASPPDAAGTAGTEGTPV
                     GAEAAGEGATAGESTTIVEASLDDILASEHAINVHESPENIQNYIACGDITGTPTNDE
                     LVIELEELNDSGFMGDALLADNGDGTTTVTITLIEMGLGVFGTPAASPAG"
     gene            2271..3869
                     /locus_tag="VGR29_07765"
     CDS             2271..3869
                     /locus_tag="VGR29_07765"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEV2073525.1"
                     /translation="MSTEPTATASPPATAELDGAVCSEFGALIAPSRPDSGGRSLGFV
                     LAILAVVSTTCWFLLYRWYGLFEHQDHAHFSFEKIPGGFDSPMMRQTAVIFLILAASY
                     VAAVRILRSAGTIVPFMKLGILLLIAGPVVANILLFPVGALDVFNYMIELKLAFHYDQ
                     NPYLVTFEDYRADSFAISAFLVNITLFYGPAWLLVSWIPTAIVGFYDVVETLLALKIF
                     NVLLLLLTSLLIAWSQEDRRWRWFTAAVFLANPLVLFEGVANAHNDVLMTAFLIGAMF
                     ALQRRSPLAGPLLALSALVKLYPVVLAPLFVVVVLRERWGWKRAGAAVLLGAITVVII
                     CAPYWEDGKLVDGLRGGLEQSQVMDHVSPFSLAQQYAQQREAQESGNPEFIRSRPSAE
                     VVPEETKRTLRNVSTGIFAAAALLIAASVWRGRPPAIAAAETLLLLLLLLTNLYGWYL
                     IPVFALLAVRLDRLSAMYISVATALGLLYYPMFIYGHFNTEWSRFQVHLFLAPFLTLP
                     ILIYLVARVLRPLITMRASKSTSE"
     gene            4219..5463
                     /locus_tag="VGR29_07770"
     CDS             4219..5463
                     /locus_tag="VGR29_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV2073526.1"
                     /translation="MHTINVTIACLAGLALLMTLFAHGLRLRWINEPLLALVLGVLIG
                     PKGLGWLDPAKYGEEREIIETVARFTLVIVLVAVGVELRGYVSAQWRPLSVLVLGGAA
                     LMWAVSTLLVWWIMGLDLLPSMLIGAVLAPTDPILSATVSTGRVARETLPERTRRLLS
                     AESSARHGLGLVFVLLPALLIMEPDREAWRHWLTDVLLWKGLVAVIVGGIVGYAVGRV
                     QAWSATQDYAETMTGPLSALFLALALGLASAVELMESDGALAVLVAGVVYARVRSKGA
                     AENKLDEEHRDYQHVIKQVLQVPIFALLGAALPWAQWQELGWKGPGLVVAVLLLRRIP
                     AILLLKPVVSQIRSWNEALFVGWFGPVGVGALFFAAVATKETHDEQIWTIATLIIAAT
                     IVAHDLTATPLSQWLGRRTSRE"
     gene            5706..>6660
                     /locus_tag="VGR29_07775"
     CDS             5706..>6660
                     /locus_tag="VGR29_07775"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEV2073527.1"
                     /translation="MDSVRPISTFPELQRLPTQVPGLDTILGGGLMVGDAYLVAGEPG
                     TGKTTLGNQISFAHAAAGGTVVFATLLTESHDRMLAHLHDFDFADRSLVGDRVHYISL
                     LGSLQEGGFEELMRLLVETLRTHRATLLVIDGAGAARMLADSDFDYARFMHGLQARTA
                     LLNCTMMLLAGEREADVAATHVDGLIQLFNRPTNARDTRWVRVAKLRGSNYLNGQHRF
                     AIGSNGITVFPRLESASAEAEAAWFQPHERLPFGVPGLDAMVAGGPQEGSATLAIGTA
                     GAGKTLLGLHFLAEGARRGEQGLIASFNETPRALAATADGAG"
BASE COUNT         1237 a         2086 c         1943 g         1394 t
ORIGIN      
        1 ggtaataggt cttcgatggg aggacgtaga tttcgaccaa ggcactatcg cgattcgcaa
       61 gcaacttcag gtgattaatg gacaaccaac gttcacagag ccgaagagca agatgagtcg
      121 ccgcaccatt ccccttcccc caaccgtcat ggatttgctc cgtcagcacc gccgacaaca
      181 gctagaggaa cgtatgcggg ccggtgaacg atggcaagca caatggggtc tcgtgttttg
      241 tagatcgacc ggtacaccac tcgataacag caacctccga aagcacttca agcggaagct
      301 cgaaggcgct ggcttgccgc ccatgcgttt ccatgacatg cgtcattcgg cagcgtcaat
      361 gctcgcagcc cgaggcgtgc atccatccgt ggcacagcga atactcgggc atgcgaacat
      421 caacacgaca ttggctgttt acacccacgt tgaagacgcg atgatgcgac aggcaactga
      481 tctcatggga gacctatacg agacagaaat ggaagcaaat tagcaggaat ggctgcaggt
      541 ttggctgcaa aggatttcgt agcggttatt ccaaaggggg agagataaac agaaatccct
      601 aatttatcag ggattcccag ggtcaagttg aaagaaactt gatgcctctg ccttcttatc
      661 gcggatagac ggggtatccc ggatacgtgt agccacccga ccagtacggc gagtagcccc
      721 accaaccata gacacccggg tagtagtcgg gagcctcggc gaggtccggg ctgtagccgg
      781 gggcatccga gagcttgctt cgttcacgat cgatgtgaac gtgctcgctg tcaatgcgag
      841 taattgcgtc gaccgggacc aggaagtgct cctcgccgat ccccaggaac ccaccgtgag
      901 cgattcgaag aaagcggacc cggttttcgg tgtcgtcaac cagcaggtcg tcgaccgtcc
      961 cgatttcctc gccgttccgg tccaggagct tcagtccccg cacgtcctcg gcaggatcag
     1021 ccactgtcag tccagtgtcg ctcagcctca taagggttcc aacgttattg ctttgggtat
     1081 tggtgtttgc catgatgtct ttcctttcct tgcgtcatgt ccctcctaca ggatcagtcg
     1141 ccgccaccgg gaaacgtcgt gtattcccgt acaactaggc gccgcaaacg ggatgccatg
     1201 gtcctcctcc aggcaaaggg tcaagacaat cctgtcgtat cccgctgcca tgtatatctc
     1261 gacctggata accgtgtgtc cctaccgaaa ccaccccggg aaagaaaaag agggcctcac
     1321 gtggcatacg tcgtgcttgt ttagcaagtc cggatggtgt catcacggaa cacaacaggt
     1381 ttatttaatg aggaggacgg caatgggcaa acgcgtgttt gcgcccgtgg cacttgtggc
     1441 cgcagcattt atcttcatga tgagcttcgt gctcggtgga gcgaccagtg cgcagggcac
     1501 cgctacccct gcagatggag gggtggaagt tgatccttct cacccggcgc acatccattc
     1561 gggcacctgt gaggaactcg gcgatgtcgt cttccctctc aacaacgtta cgccgctcgg
     1621 tgtcgacgtc tcccctgcta cgccggtcga catgaccgcg tccccgcctg atgcggccgg
     1681 aactgccggg acagagggca caccggtagg ggctgaggca gccggtgagg gcgcgacagc
     1741 tggggaaagc actacgatcg tggaagcgtc tcttgacgat atcctggcct ccgagcatgc
     1801 gatcaacgtg cacgagagcc cggagaacat ccagaactac atcgcctgtg gcgacatcac
     1861 tggcacaccg acgaacgacg agctcgtgat tgagctcgaa gagctcaatg actcgggctt
     1921 tatgggtgat gctcttctgg ccgacaatgg tgacggcacc acgaccgtga cgatcacgct
     1981 gattgagatg ggcttgggag tcttcggcac cccagccgcc tctcctgcag gctgacatcc
     2041 gaacgctgta ccgaagtcac agcaagcaat ggccacggaa agttccgtgg ccattgctgc
     2101 gcgcctttcc cctcttctcg cggcgccagg gaacattcac cccgtttact cgtgctcctt
     2161 gccttcctgc ccggcaacat ggcatacagt gtcggcattg agagcatgga cagtcgcttt
     2221 cgacggccct ttcgtgatgc ggtacagacg agacaaagat tgacgaggca atgtcgaccg
     2281 agccgactgc aaccgcatca cccccagcca ctgccgagct cgatggagct gtctgctcgg
     2341 agtttggtgc cctgattgct ccatctcggc cggattctgg gggtcggagc cttggcttcg
     2401 ttctggcaat cctggcggtt gtcagcacca cttgctggtt tctcctctac cgctggtacg
     2461 ggctgttcga gcaccaggac catgcgcatt tttcattcga gaagattccg ggcgggtttg
     2521 attcgcccat gatgcgccag accgccgtga tcttcctgat cctggccgcc tcctacgtcg
     2581 ccgcagtgag gatccttcga tccgctggaa ccatcgtccc gttcatgaag ctcggaatcc
     2641 tgctcttgat tgccgggccc gtcgttgcga acatcctgct ctttccagtc ggcgctctcg
     2701 atgtcttcaa ctacatgatc gagttgaaac tcgcctttca ttacgaccag aatccctatc
     2761 tcgtcacctt cgaagactac cgggccgact cattcgcgat ctccgcgttc ctcgtcaaca
     2821 tcacactctt ttacgggccg gcttggctgc tcgtctcgtg gatcccaaca gccatcgtgg
     2881 ggttctatga tgtcgtcgag acactcctgg ctctcaagat cttcaacgtt ctcctgctcc
     2941 tgctcacctc gctcctgatc gcgtggtctc aggaggaccg ccgctggcgt tggtttacgg
     3001 ctgccgtctt cctggccaac ccgctcgttc tctttgaggg tgtcgccaac gcccacaacg
     3061 atgtgctcat gacggccttc ctcatcggcg cgatgttcgc gctgcagcgg cgatcacccc
     3121 ttgccggacc actgctggcg ctctctgcac tcgtcaagct ctacccggtg gtgctggcgc
     3181 cgctctttgt cgttgtggtt ctcagggaac gctgggggtg gaagcgggct ggcgccgcag
     3241 tgcttctggg tgcgatcacg gtcgtcatca tctgcgctcc ctattgggag gatggcaagc
     3301 tggttgatgg cctcaggggc ggactcgagc aatcacaggt catggaccat gtctcgccct
     3361 tctcgctcgc ccagcaatac gcgcagcaac gtgaggcgca agagtcaggc aacccggaat
     3421 ttatccgcag ccggccaagc gcagaggtcg taccggaaga aacgaagcgc accttgcgca
     3481 atgtgtctac tgggatcttt gccgcagcgg cgctgctgat tgcagcatca gtctggaggg
     3541 gaagaccacc ggcaatcgcg gcagcggaaa cactactcct cctgctgcta ctcctgacga
     3601 acctctatgg ttggtatctc attcccgtgt tcgcgctcct ggcggtgcga ctcgatcgcc
     3661 tcagcgcaat gtacatctcg gtcgcgacag cgctgggcct cctctactat ccgatgttca
     3721 tctatggcca cttcaatact gaatggtccc gcttccaggt ccacctcttc ctggcgccct
     3781 tcctcaccct gccgatcttg atctatctgg tggcccgcgt gctccggccg ctcattacca
     3841 tgcgggcttc gaagagcacc agcgaataat cgatcctcca ccctcatccc gacgcgtccc
     3901 taccggggcg gaagccagcg cttcaccgct ggacgggcat cgtcgccact cggctcgcag
     3961 cctggactgg gacccgcttg gaacgtgtgt tcatgcgaag gcgtctagct cctgatctca
     4021 attgcctcgg cgcgtacgct gctttggctc ctcgccagcg cccgacgacc ggctgcccgc
     4081 tggcgaactg agactccctc aaaaacctcg tcgcacgtaa cttgcgcggg cgggtccaga
     4141 ggcatcactt tgcgagaagc gagccgactt cgcaagcgga gcgccaagca tggagaactc
     4201 aacaccgggg aggtggatgt gcataccatc aatgtgacga ttgcgtgcct cgcaggtctc
     4261 gcattgttga tgaccctgtt cgcgcatgga cttcggcttc gctggatcaa cgagcccctg
     4321 ctcgcgcttg tgctgggggt actgatcggg ccgaaggggt tggggtggct cgaccctgcc
     4381 aaatacggtg aggaacgcga aattatcgaa accgtggccc ggttcactct ggtgatcgtg
     4441 ctcgtggccg ttggcgtcga gctgcgcggt tacgtctcgg cccagtggcg tcccctcagt
     4501 gtcctggtcc tcggcggcgc ggccctcatg tgggccgtca gcacgttact cgtctggtgg
     4561 attatggggc ttgacctact cccctccatg ctcatcggcg ccgtgctggc accgaccgat
     4621 ccaatcctct ccgcgaccgt ctcgacaggc agggtcgccc gcgagacact ccccgagcgg
     4681 acgcgccgtc tgctgtctgc cgagtcatcg gcacgtcatg gcctgggtct cgtcttcgtg
     4741 cttctccccg ccctcctgat catggaaccg gaccgcgagg catggcgaca ttggttgacc
     4801 gatgtgctgc tctggaaagg gctggtcgcc gtgatagtcg gtgggatcgt tggctacgct
     4861 gtcggccgag ttcaggcctg gagcgctacg caagactatg ccgagacgat gaccggcccg
     4921 ctgtcggcgc tcttccttgc gctcgcgctt ggtcttgcct cagcggtgga gctgatggag
     4981 agcgacggcg cccttgccgt acttgtcgct ggtgttgtgt acgcgcgggt gcgctccaag
     5041 ggtgcggcag agaacaagct cgacgaggag caccgcgatt accagcatgt gatcaagcag
     5101 gtgcttcagg tcccgatctt cgcgttgctc ggagcggcgc taccgtgggc gcaatggcaa
     5161 gagttgggct ggaagggccc cggcctcgtg gtggcagtcc tgctcctgcg acgcatcccc
     5221 gccattctcc tcctgaagcc ggtcgttagc cagatacgga gttggaacga ggcactcttc
     5281 gtcggttggt tcggcccagt gggtgtgggc gcgctctttt tcgccgccgt ggccacgaaa
     5341 gagacacatg acgagcagat ctggaccatt gcgacactga ttattgccgc cacaatcgtc
     5401 gctcacgacc tgacggcaac acctctgagc cagtggctgg gccgccggac atcccgcgaa
     5461 tagactcaca gggacggaca agagacaaca tcgccaggcc cgccgtgatt caggaacctt
     5521 gagagcccta ccgcgccgag gcgagcgggg gacacggttg acagccctgt accctccatt
     5581 tatggcggcg cgcacaaccc ggttttcact gccaccatgg gcgaaacgta cagtacactg
     5641 tgaccatatg gtcggtccca gacgcaccgc aattcactat tgcaccaccc aggcaggaga
     5701 cgcccttgga ctccgttcgt cccatctcaa ccttcccgga gcttcagcgg ctccccactc
     5761 aggttcctgg cctggacacc attctcgggg gcggcttgat ggttggggat gcatatctcg
     5821 tagctggcga acccggcact ggcaagacga cacttggcaa tcagatatcc tttgcccatg
     5881 ccgctgccgg tggcacggtg gttttcgcca cactcctgac cgaatcgcac gatcggatgc
     5941 tggcacatct gcatgatttc gatttcgccg accgctcgct cgttggagac agggtgcatt
     6001 acatcagcct tctcggctcc ctgcaggaag ggggcttcga ggagctgatg cggctgctcg
     6061 tcgaaaccct gcgcactcac cgggcaaccc tccttgtcat tgacggcgcc ggagccgccc
     6121 gcatgctcgc cgactccgat ttcgactacg cgcggttcat gcacgggttg caggcgcgga
     6181 ctgccctcct caactgcacc atgatgctgc ttgccggtga gcgcgaggca gatgtcgccg
     6241 caacacacgt ggacggcttg attcagctct tcaacagacc gaccaatgcg cgtgacactc
     6301 gctgggtgcg agtagcgaag cttcgcggct ccaactacct caatgggcag caccggtttg
     6361 cgatcggcag caacggaatc accgtatttc cccgccttga atcagcgtcg gcggaggcgg
     6421 aggcagcatg gttccagcct catgagcgcc tgcccttcgg tgtccccggc ctcgacgcaa
     6481 tggtggcggg cggcccccag gagggcagtg ccaccctggc catagggact gcaggcgccg
     6541 gcaagacgtt actcggcctg catttcctgg cagagggtgc gcgccgcggt gagcagggcc
     6601 tcatagcgag cttcaacgaa accccacgcg cccttgccgc taccgcggat ggcgcgggaa
//
LOCUS       DASYWM010000187         9697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_232775, whole genome shotgun sequence.
ACCESSION   DASYWM010000187 DASYWM010000000
VERSION     DASYWM010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232775"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VGR29_07780"
     CDS             <1..495
                     /locus_tag="VGR29_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2073528.1"
                     /translation="IRIPVHMIETINKIMRTSRQMQQELGREPTHEELGKALELPPER
                     IREILKLSQDPVSLEMPIGEEEDSTLGDFIEDEKLPAPADAAGRQLLREQVEEVLHTL
                     SDREREVLYMRYGLDDGRSRTLEDVGKHIGVTRERIRQIEAKALRKLRHPSRANILRD
                     YLDE"
     gene            610..2001
                     /gene="obgE"
                     /locus_tag="VGR29_07785"
     CDS             610..2001
                     /gene="obgE"
                     /locus_tag="VGR29_07785"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV2073529.1"
                     /translation="MPLVDTARIFVQSGKGGNGSTHFRREKYVPKGGPDGGDGGRGGD
                     VILRVKDNLNTLLPFIYVNKYVAKDGGAGTGQKKFGKAGASVYVDVPPGTVVFLDETD
                     EQIADLTEPGEEFLIARGGKGGLGNVHFKSSTRQAPRIAELGEPGEEYWLRLELRLIA
                     DVGLIGLPNAGKSTLLASSTRARPKIADYPFTTLQPNLGVVEVGGQGGDTFVIADIPG
                     LIEGAAAGHGLGHEFLRHVRRTKLLVHVLDASGGLEGRDPLEDFRKINAELFEFDPEM
                     ARKPMIVALNKVDLVEARENLPRLQQVLGDEGHSIFEISAATGEGVTELQREMSSRLR
                     EIREAEAEEKRLQEEKPKRRVYTIGDVDERAWDITRVEQDRFLVTGVGIERFTKMTNF
                     DQWESVERFQRVLDRAGISDELNKMGIEEGDTVQIGDFEMTWGDEGEDNPWLAGEDER
                     LAKLLAEEGYDEA"
     gene            complement(2039..2785)
                     /locus_tag="VGR29_07790"
     CDS             complement(2039..2785)
                     /locus_tag="VGR29_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV2073530.1"
                     /translation="MTSRDEQQMDLRKGTMSMLTFRILRPTLYGLCKILVRLDVQGAR
                     NVPKGDGCIIVSNHLHNLDPVLISIACPRPLHYMAKIELMRVPVIGRILRWVGAFPIN
                     RGKMDRPAIKRAQATLQQDIALGMFPEGTRSTAMKIQRVLPGAGLLALRDGVKIVPTA
                     ITGTERLPFNGIKQQRDHASMPDPGHKGVRIVFGDPFVIPGEIDGKRTGAAGATDHMM
                     QRVAALLPDAYRGIYCTKQSEIIEVEDEIP"
     gene            complement(2782..3486)
                     /gene="cmk"
                     /locus_tag="VGR29_07795"
     CDS             complement(2782..3486)
                     /gene="cmk"
                     /locus_tag="VGR29_07795"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV2073531.1"
                     /translation="MTDHDHRRVIAIDGPAAAGKSTVARCLADQLGIMLFDTGALYRA
                     VTLAALRGDIDLADETALAQLAVDLHIDLRPASVEDDRLADVILDGEDVTWEIRSPAV
                     DANVSRVAAYPAVRDALLAVQRNIADGSEVVMVGRDIGTIVTPRAGTKIYLDASVEER
                     ARRRHQDLRKQGVATDFLSVMEDLRERDYKDSSRTTAPLRAANDAVRIVTDGLSVDEI
                     VLSIARLVRERWGEAA"
     gene            complement(3428..4549)
                     /locus_tag="VGR29_07800"
     CDS             complement(3428..4549)
                     /locus_tag="VGR29_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEV2073532.1"
                     /translation="MTPRAQQEPSEDQDQEKTGERLQRVLAARGVASRRASEALITAG
                     RVQVNGQTVTELGTRVHPTRDEIRVDGKPVRRQKPRFIVLNKPSGFITTVSDEKQRWT
                     VMDLIDVQERVYPVGRLDRETQGLLLLTNEGDVANRIMHPKFGLAKEYHVITGKKPND
                     RQLQRIRDGIEIQGRTIVPDECRLLREGPEGVIVKIVLHEGKYHVVRQIMDAVNIEVV
                     KLRRVRIGPLTLTGIPPGAWRDLTPGELSQLYEALGLPAEEADKMNARRPQQLLPVGG
                     FQHTPQQGNTKRSSSPSHEGGTGPRHQGSESTRQERGRRPGWGDGRPQERRGDRRSSS
                     PDGRPGDPRRTNSRTSPGRDHDGSRPPKGHRNRRPGGGR"
     gene            complement(4552..5772)
                     /locus_tag="VGR29_07805"
     CDS             complement(4552..5772)
                     /locus_tag="VGR29_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073533.1"
                     /translation="MPLPVPSLRLPAMRCLLPILIAFALLVSTASATPGLATQPGVTM
                     AISPPEVTARSVYVVDMTSGVELYAKDPYAHLQVGSIVKIATALVVINNVGLDEQVVI
                     QESDLVDVDEYSNMALVAGDTLTVRLLLYGLLIPSGNDAARALARHVGAKLSGSEDAR
                     IATEAFVKEMNAYAATLGLKDSRFTVPDGIDTPNSYSSAYDVTILARELMKNETLRSI
                     VREPAYRFVSVGPEARVYEKSTTNNRLGQSAVVGVKTGTTLMAGGNVVLAREVNGGAN
                     TVLITIIGAEHTYTTGNADTPDARWTDADTIMSAMDASFTWTAPDADDVLPGLSEELA
                     VWNLQFQDPPAIPIPTGGTAPLGYQLQVGAPADPGARSGALYLYYGEDRVGSIPIFQA
                     GEPAAWDPSKRIAA"
     gene            6029..6877
                     /locus_tag="VGR29_07810"
     CDS             6029..6877
                     /locus_tag="VGR29_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV2073534.1"
                     /translation="MTPNSIQPVRIAAVGDLHIRTHIPEELQEDLIALNDRVDLIVVA
                     GDITDGGRIPEVELAAEMFSPIRVPIVAVLGNHDRRGLRRTVMRRVLGASGLQLLDGD
                     AVLLQLADGRRVGVAGISGTGGGFYLDEADAVIGGRLRQAVAIKARREAARLEQALET
                     LEAGCPDVTIALTHFAPTITTLGEEPPLKYWMLGNSLLGRIIDGHPVDLVVHGHAHIG
                     NEVGATPGETPVRNVALPVTGGIVILEVGPGQQVRSVGGRRAPSTSHDLPRIPGANMM
                     DVLRII"
     gene            6944..7951
                     /locus_tag="VGR29_07815"
     CDS             6944..7951
                     /locus_tag="VGR29_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2073535.1"
                     /translation="MPSVSRPKTRIRLDVLTNPYGPTEQVFDAIASDDPGAVADSELA
                     DRLREQLGLLAGVPASWVVLANGIDDLHSMIARWRSEQGPLVVFPPGDLNLETWIDQY
                     GAQVERIPRLAGFRLPIEPDDSGLPKGATAIVMSPNDPSGTIITVQDVVRLVRQCSAV
                     VIDERHAAYSSRSILPLVREFETLIVVQTMETFAGLSSLPLAWAVAPPRMAAEIARRG
                     RPSGVGRLPLLAALATLEDYARVMRTVQWVMNEKSRLFRQIRKLNMISPPYPSWGNFL
                     LCRFERGSSAFFVPRLAERGIDVYRPPHPNLRNHVRISAVSADATNALKQALIDIALA
                     L"
     gene            complement(7966..8703)
                     /gene="rph"
                     /locus_tag="VGR29_07820"
     CDS             complement(7966..8703)
                     /gene="rph"
                     /locus_tag="VGR29_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEV2073536.1"
                     /translation="MTITIPMRRSYGRATDGLRPLSIAPNALPHAEGSAYIKIGDTHV
                     LCAATVEDNVPRWMKGTGKGWVTAEYALLPRATQERTQREAVKGKQGGRTVEIQRLIG
                     RSLRAVTDMEALGERQIILDCDVIRADGGTRCAAITGAYVALALACRRMMDEKRLRRY
                     PLKSAVGAISVGVVKGVPLLDLDYNEDSKADVDANIVMTDENAFVELQGTAEKNPFSP
                     AILQVMLDLASSGLEEIFVAQKKAIEG"
     gene            complement(8700..>9697)
                     /locus_tag="VGR29_07825"
     CDS             complement(8700..>9697)
                     /locus_tag="VGR29_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV2073537.1"
                     /translation="AQQDAILPHVEDGTVILIGATTENPSFEVNAPLLSRSRVMTLRS
                     LADEDVMAILLRALADKERGLGNDELMITDDGLELLANMANGDARFALNTLEFAATGV
                     GEDRQISADLVREAAQRRAATYDKGGNDHYDTISALHKTIRGSDADAALYWLARMLER
                     GDDPLYVARRLVRFASEDIGLADPAALELAMAAQQAVHFLGMPEGALALAELTVYLAL
                     APKSNAIYRAYGAVQRDVAESRNDPVPIHLRNAPTRFMKNEGFGKGYKYAHDFEGGIV
                     GQVNVPENIQGRRYYEPTDRGLEHEFGNRMKRIQDVYRRTQQQGHTPRPKDQESP"
BASE COUNT         1822 a         2869 c         3052 g         1954 t
ORIGIN      
        1 atccggatcc cggtgcatat gatcgagacg atcaacaaga tcatgcgcac gagccggcag
       61 atgcagcagg agctgggccg ggagccgacg catgaggagc tgggcaaggc gcttgagctg
      121 cccccggaac gaatccgcga gattctcaag ctgagccagg atccggtgag ccttgagatg
      181 ccgatcggcg aggaggaaga cagcacgctc ggcgacttta tcgaggacga gaagttgccg
      241 gccccagccg atgcagccgg acgccaattg ctgcgcgagc aggtagagga agtgctgcac
      301 accttgtcgg accgggaacg ggaagtcctc tacatgcggt acggcctgga cgatggacga
      361 agtcgcacgc tggaggacgt aggcaagcat atcggggtca cgcgagagcg aatccgtcag
      421 attgaggcga aggcgctccg gaagctccgt catccgagcc gcgcgaacat cctgcgagat
      481 tacctggacg aatagccccg gggatagggg cggatacacc ggtgaacccg ttgcgcagaa
      541 aatgaggcgg gtttattcgg cgtatcctgt atcagtacaa gcaatgatga ttctaagcga
      601 ggacagtaga tgccattagt cgatacagca cgaatctttg ttcagtcggg caagggaggc
      661 aacggctcca ctcacttccg ccgtgagaag tacgtgccca aaggcggacc ggacggtgga
      721 gacggagggc gcggcggtga cgtgatcctt cgggtcaagg acaacctgaa cacgctcctg
      781 ccatttatct atgtcaataa atacgttgcc aaggacgggg gagcaggtac cggacagaag
      841 aagttcggca aggcaggagc ttcggtctac gtcgatgttc cgccgggaac ggtcgtattt
      901 cttgacgaaa ccgatgagca gatcgccgat ctcacagagc caggtgagga attcctgatt
      961 gcccgcggcg gcaagggagg cctcggcaac gtccacttca agtcctcaac gcgccaggcc
     1021 ccgcgtatcg ctgagttggg cgagcctggt gaggagtatt ggcttcggct ggagctcagg
     1081 ctaatcgccg atgtcggtct gatcggcctg cctaatgccg gcaagtcgac actgctggcc
     1141 tcatccaccc gcgcgagacc caagattgct gattacccct tcaccacctt gcagccgaat
     1201 ctcggcgtgg tagaggtggg aggacagggt ggggatactt ttgtgatcgc ggatatccct
     1261 gggcttattg aaggagccgc agccggacat ggcctcggtc acgaattcct ccggcatgtc
     1321 cggcggacca agttgcttgt ccacgtgctc gatgcatccg gtggccttga gggacgagat
     1381 cctctggagg attttcggaa gatcaacgcc gagctcttcg agtttgaccc ggaaatggca
     1441 aggaaaccaa tgatcgtcgc gctcaacaag gttgacctgg ttgaggcgcg ggagaatttg
     1501 ccgcgacttc agcaggtgct cggagatgag ggccactcga tcttcgagat ttcggccgcc
     1561 accggtgagg gcgtcacgga gcttcaacgt gagatgtcat cccgcctgcg ggaaatccgc
     1621 gaggccgagg ctgaagagaa gcggctccag gaagagaagc cgaagcgccg cgtctacaca
     1681 atcggtgatg tcgacgagcg ggcatgggat atcacgcgcg tcgaacagga tcgcttcctg
     1741 gtgaccggtg ttggcatcga gcgcttcacc aagatgacca acttcgacca gtgggaatcc
     1801 gtagagcggt tccagcgcgt tctggaccgg gcgggtatct cggacgagct caacaagatg
     1861 ggcatcgaag agggtgacac ggtccagatc ggtgatttcg agatgacctg gggcgatgag
     1921 ggtgaggaca atccctggct tgccggggaa gacgaacgcc tggcgaagtt gctcgctgaa
     1981 gagggatatg acgaagcgta gatgccgtcg cctggatgac gtatcctgcg aacggcgatt
     2041 atggaatctc gtcctccacc tcgataatct ctgattgttt ggtgcagtat atgccgcggt
     2101 atgcatcggg aaggagcgcg gcaacccgct gcatcatgtg gtctgtcgca cctgcggcgc
     2161 cggtccgttt gccgtcaatt tctcccggga ttacaaacgg atcaccgaac acgatgcgaa
     2221 cgcccttgtg cccggggtcc ggcatagagg cgtggtcacg ttgctgcttg atgccgttga
     2281 aggggaggcg ctccgtcccg gtgatggccg tcggaacgat cttgacgcca tcccgtagtg
     2341 caagaagacc ggctcccgga agcacccgct gaatcttcat cgccgtggag cgcgttccct
     2401 cggggaacat tccgagtgca atgtcctgct gcagtgtggc ttgcgcgcgc ttgatagcag
     2461 ggcggtccat ttttccacga ttgatcggga aagcgccaac ccacctcagg atgcggccga
     2521 tgaccgggac tctcatcaac tcgatcttcg ccatgtagtg gagcgggcgg ggacaggcaa
     2581 tgctgatgag gactgggtcc aggttgtgca ggtggttgga gacgatgatg catccgtcac
     2641 ctttcgggac gtttctcgcg ccctgcacat ccagtcgcac caggatcttg cagagtccat
     2701 acagcgtggg ccggaggatg cggaacgtca gcatcgacat cgtgcccttg cgaagatcca
     2761 tctgctgctc atctcgtgat gtcatgcagc ctctccccag cgttcacgga ccagtcgcgc
     2821 aatcgacagc acgatctcgt cgacggacag tccatcggtg acgatacgca cggcgtcgtt
     2881 cgcggcgcgg aggggtgccg ttgtccggct cgaatccttg taatcgcgct cgcggaggtc
     2941 ttccatcacg gagagaaaat ctgtcgctac tccctgcttg cgaaggtcct ggtggcgtcg
     3001 ccgggcgcgt tcctcgacac tggcatccag gtagatcttc gtgccggccc gtggcgtgac
     3061 aatggttccg atatcccgtc ccaccatcac gacctccgag ccatccgcaa tattccgttg
     3121 gacggccagc agggcatcac gtaccgcagg ataggctgct acccgcgaga cgttcgcgtc
     3181 cactgccggg gagcggatct cccaggtcac atcctctcca tccagaatca catcggccag
     3241 tcggtcatcc tcaactgaag cgggcctcaa atcaatatgc aaatcgaccg cgagttgggc
     3301 cagggcggtc tcgtcggcga gatcaatatc cccccggagc gcggcgagcg tcacagcgcg
     3361 atagagtgca ccagtatcaa acagcatgat cccgagctgg tccgccaggc atcgggcaac
     3421 ggtcgattta ccggccgccg ccgggccgtc gattgcgatg acccttcggt ggtcgtgatc
     3481 cgtcatgatc cctcccgggt gatgttcgac tgttggtccg gcgtgggtcc ccagggcgcc
     3541 cgtccggcga ggaagatcgt cggtctccac gtcgctcctg tggccggcca tccccccacc
     3601 ctggccgccg gccacgctcc tgtcgggtag attcactacc ttggtgcctt ggccccgtgc
     3661 ctccctcgtg agacggggaa gaggatcgct tggtgttgcc ttgctgcggc gtatgctgga
     3721 agccaccgac aggcaagagc tgttgcgggc ggcgcgcgtt catcttgtcg gcttcctcgg
     3781 ccggtaggcc gagtgcctcg tagagttgcg aaagctcacc aggcgtcaaa tcccgccatg
     3841 caccgggagg gatcccggtg agcgtcagtg ggccgatgcg aacacggcga agcttgacca
     3901 cttcgatatt gacggcgtcc ataatctgcc ggacgacgtg gtacttcccc tcatgcagga
     3961 cgatcttgac aatgacccct tcagggccct cgcgtaggag ccgacattca tctgggacga
     4021 tcgtgcggcc ctggatctcg atgccatccc ggatccgctg gagctgtcga tcattcggct
     4081 tcttgcccgt gatgacgtga tattccttcg ccagaccaaa tttcggatgc atgatgcggt
     4141 tggcgacatc gccttcgttg gtcagcaaga ggaggccctg ggtctcccgg tcaaggcggc
     4201 ccaccggata gacgcgctcc tgcacgtcga tgagatccat caccgtccaa cgctgcttct
     4261 cgtcgctcac ggtggtaatg aacccacttg gtttattcag gacaatgaac cgcggcttct
     4321 gtcgtcggac cggcttgccg tcgacgcgta tctcgtccct ggtcggatgg actcgtgttc
     4381 cgagttccgt gaccgtctgg ccgttcacct gcacccggcc agctgtaatg agcgcctctg
     4441 atgcgcggcg ggaggccact ccgcgtgccg cgagaacacg ctgcagccgt tcccctgtct
     4501 tttcctgatc ctggtcttcc gatggttcct gctgtgcgcg tggtgtcatt gtcaggctgc
     4561 gattctcttt gaggggtccc aggcggcggg ttctccggcc tggaagatcg ggattgaccc
     4621 aaccctgtct tcaccataat agaggtaaag ggctccggag cgagccccgg gatcggcagg
     4681 tgcgcccact tgcagctgat atcccagtgg ggcagtgccg ccggtgggga tcggaatggc
     4741 cggtggatcc tggaactgga gattccacac cgccagttcc tcggaaagtc cgggcaggac
     4801 gtcatcggca tccggcgccg tccaggtgaa cgaggcgtcc atcgcgctca tgatcgtatc
     4861 ggcgtcagtc cagcgggcgt ccggtgtatc ggcattgccg gtcgtatacg tgtgctcagc
     4921 accaatgatc gtgatgagca ccgtgtttgc ccccccgttg acctcgcgtg ccaggaccac
     4981 gttccctccc gccatcagtg tcgtaccggt tttgacgcca acgacagcgg attggccaag
     5041 acgattgttg gttgtgctct tctcatagac gcgagcttcc ggaccgaccg agacaaagcg
     5101 gtacgcaggc tctctcacaa tgctgcgcaa ggtctcattc ttcatcagtt cccgggcaag
     5161 aatcgtgaca tcataggcag aggagtaact gttgggcgtg tcgatcccgt ccgggacggt
     5221 aaagcggctg tccttcaatc ccagtgtggc ggcataggcg ttcatctcct ttacgaatgc
     5281 ttcagttgca atacgggcat cttcgctgcc gctgagcttg gcgcccacat gtcgggcaag
     5341 ggcacgggca gcatcgttgc cagaaggaat caggagcccg tagagcagta ggcgaacggt
     5401 aagggtgtcg cccgctacga gcgccatatt cgagtactcg tcaacatcga ccaaatccga
     5461 ttcctggatg accacctgct catccaggcc gacgttattg atcaccacca atgccgtggc
     5521 gatcttgacg atacttccca cctggagatg ggcgtaggga tccttcgcat aaagctccac
     5581 gcccgaggtc atatccacca catagacgct cctggccgtc acctctggcg gagagattgc
     5641 catcgtgacg cccggctgcg tcgcgagccc gggagtcgcc gacgccgtcg acaccagcag
     5701 tgcaaaggca atgaggatgg gaagcaggca gcgcatcgcc gggagtcgta atgatggaac
     5761 aggcaagggc atgaaacgcg tctctttcgg tctctcgtca gggcaggagt ggaacgggct
     5821 ggcacacaga gaccctgctg tttgaatcgt acgaactcca gcacggtccc actggcttca
     5881 gtatatgagc cacgtacggt accggaccac ccgactgaac cgcctcgtcc gtggggtgcg
     5941 gtaaacttcg gggataggct ttgagtggtg accagtgacg tactttccgg caggcagggc
     6001 caaaacgaaa actcccaagg atgccgtgtt gacacccaac tcgatccagc cggtacgaat
     6061 tgccgctgtg ggcgatctcc atattcgtac ccatatcccc gaagaacttc aggaggacct
     6121 gatcgcgttg aatgatcggg tcgatctcat tgtggtcgcc ggcgatataa ccgatggtgg
     6181 gcggatacct gaggtggagc ttgccgcgga gatgttttca cccatccgcg tgccgatagt
     6241 ggcggtactc ggcaaccatg accggcgagg gctacggcga acagtgatgc gccgcgttct
     6301 cggagcgtca gggttgcaac tactcgatgg agatgccgta ctgctccagt tggccgatgg
     6361 tcgccgcgtc ggcgtggcgg gcatcagtgg tacgggtggt ggattctatc tggacgaggc
     6421 tgatgcggtg attgggggtc gcctccggca ggcagtcgcc atcaaggcac gccgggaggc
     6481 cgcacgtttg gagcaggcgc tggagaccct tgaggcgggc tgcccggatg tcaccattgc
     6541 gctgacccat tttgcgccga cgatcacgac gctcggcgag gagccgccgc tgaagtactg
     6601 gatgctgggc aactcgcttc tggggcgtat catcgacgga caccctgttg atctcgtcgt
     6661 gcacggtcat gcgcatatcg ggaacgaagt aggagcaaca cctggtgaaa cgccggtgcg
     6721 caacgttgcc ctgccagtga cgggcggaat cgtgatcctt gaggttggac cggggcagca
     6781 ggtacgctca gtgggcggta ggcgcgcgcc ttcgacctcg cacgatctcc ccaggatccc
     6841 cggtgccaat atgatggatg tacttcgcat catctgaggt ttcaggcacc gcacgatcta
     6901 cgctcttctc atattgcatt caccgtgggg tagccgcagt ttgatgccat ccgtttcacg
     6961 accaaagacg cgtatccgcc tcgatgtgct caccaacccg tacggtccca ccgagcaagt
     7021 tttcgatgcg atagcgtctg atgatcctgg tgctgttgcc gattccgagc tcgcggaccg
     7081 gctgcgcgaa cagctcggtc tgctcgccgg tgttcctgcg tcatgggtcg tactcgcgaa
     7141 cggcatcgac gatcttcatt caatgatcgc ccggtggcgc tcagagcaag gcccactggt
     7201 tgtcttccca cccggcgacc ttaatttgga gacctggatc gatcaatacg gtgctcaggt
     7261 ggagcgtatt ccccgcctcg ccgggtttcg tctcccgatc gagcccgacg attctggtct
     7321 tcccaaggga gcgaccgcga tcgtgatgtc gccaaatgac ccttccggga caatcattac
     7381 ggtgcaagat gtggtgcgac tggtgcgcca gtgctctgcc gtggtgatcg atgaacgcca
     7441 cgcggcgtac agctccaggt cgattctccc cctggtacgg gagttcgaga cattgatcgt
     7501 ggtgcaaacg atggagacgt ttgccgggct gtcgtcgttg ccactggcct gggccgtggc
     7561 gccccccaga atggcggccg agatcgctcg acgcggacgg ccctcgggtg ttggccgcct
     7621 gccacttctt gcagcgctgg cgacacttga ggattacgct cgggtgatgc gtactgtgca
     7681 gtgggtgatg aacgagaaga gccgcctctt tcgccagatc cggaagctga acatgattag
     7741 ccctccctat ccgtcgtggg ggaatttcct gctgtgccga ttcgagcgag gatcatcggc
     7801 cttctttgtg ccccggctgg ccgagcgtgg gatcgacgtc taccggcctc cacaccccaa
     7861 tctgcggaac cacgttcgca ttagcgccgt gtcggcggac gcgacgaacg ccctcaagca
     7921 ggcactcatc gacatcgcgc tcgcgttgta gggctgcgca gcgttctatc cctcaatcgc
     7981 tttcttctgc gcgacgaaga tctcttcgag ccccgaggaa gcaagatcga gcatcacctg
     8041 gaggatggcg ggggagaagg ggtttttctc cgcggtgcct tggagttcga cgaacgcgtt
     8101 ctcgtccgtc ataacgatgt tggcgtccac gtcggccttc gagtcctcgt tgtagtcaag
     8161 gtcaagcagg ggaacgcctt ttacgactcc cacgctgatg gctccaaccg ccgacttgag
     8221 cggatagcgt cgcagacgct tctcatccat catgcggcgg caagccaggg caagggcgac
     8281 ataggcgccc gtgatcgccg cgcagcgtgt gcccccgtcc gcgcggatga catcgcaatc
     8341 caggatgatc tggcgctcac ccaaggcttc catgtccgtg accgcgcgca gggagcggcc
     8401 gatcaggcgc tggatctcga cggtcctgcc cccctgcttg cccttcaccg cctcgcgttg
     8461 cgtgcgctcc tgtgttgcac gggggagaag ggcgtactcc gcggtgaccc aacccttgcc
     8521 ggtacctttc atccaccggg gcacgttgtc ctcgacggta gcggcgcaca gcacatgggt
     8581 gtcgccgatc ttgatgtagg ccgaaccttc ggcgtgcggc agcgcatttg gagcgatcga
     8641 gagcggtcgg agcccatcgg tagcacggcc gtaagatcgc cgcatcggga tggtgatggt
     8701 catggagatt cctgatcctt cggacgaggc gtatgcccct gttgttgtgt acgacgatag
     8761 acgtcttgaa tgcgcttcat acggttgccg aactcgtgct caaggccacg gtcggtgggt
     8821 tcgtagtagc gccgcccctg tatgttctcg gggacattaa cctgaccgac gatgccgccc
     8881 tcgaagtcgt gcgcgtactt gtatcccttg ccgaacccct cgtttttcat gaaccgggtg
     8941 ggggcgttgc gcaggtgtat tggaacggga tcgttgcggc tctccgcgac atccctctgg
     9001 acggccccat aggccctgta gatggcgttg ctcttcggag ccaacgccaa atagacggtg
     9061 agctccgcca gcgccaatgc gccctccggc atcccaagga agtggactgc ttgctgggcc
     9121 gccatggcca gctccagggc agcagggtcg gccaggccaa tatcctccga ggcaaacctg
     9181 accagcctgc gggcaacata gagtggatcg tcaccgcgtt cgagcatccg tgcaagccag
     9241 tagagggcgg catcggcatc ggagccacga atagtcttgt gcagtgcgga gatggtgtcg
     9301 tagtggtcat tcccgccttt gtcatacgtt gcggcacggc gctgggcggc ttcgcgcacg
     9361 agatcggcac tgatctgccg atcctcgcct acgccggttg cagcgaattc cagtgtattg
     9421 agcgcgaacc gggcatcgcc gttcgccatg ttcgcaagca gttccaggcc atcgtcggtg
     9481 atcatcagtt catcattgcc caggccccgc tccttgtccg ccaatgcccg gagcaggatg
     9541 gccatgacgt cttcgtcggc aagggagcgg agcgtcataa cgcgggagcg ggagagcagt
     9601 ggggcgttga cttcaaacga ggggttctcg gttgtggcgc cgatcaggat gaccgtgccg
     9661 tcctcaacgt gaggcaatat cgcgtcctgc tgggcct
//
LOCUS       DASYWM010000188         4527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_64397, whole genome shotgun sequence.
ACCESSION   DASYWM010000188 DASYWM010000000
VERSION     DASYWM010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64397"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VGR29_07830"
     CDS             complement(<1..273)
                     /locus_tag="VGR29_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2073538.1"
                     /translation="MAIPTRPFGGTGDQVSILGLGGAHIGKPSEDEAIRIVHEAIDAG
                     VTFLDNAWEYNERESERRMGKALAQGGYRARAFLMTKDCAHDRRAAH"
     gene            complement(668..1021)
                     /locus_tag="VGR29_07835"
     CDS             complement(668..1021)
                     /locus_tag="VGR29_07835"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2073539.1"
                     /translation="MVEVKLGSLAIDCPNPLELATFYSRLLGLEMNGEDAVLTPEGRE
                     IWFQKVDDYQAPTWPTQERGQQMHFDFTVRDIEEAQAYAIELGAKLVDDGQESFHVLL
                     DPAGHPFCLCKQRNE"
     gene            complement(1434..2171)
                     /locus_tag="VGR29_07840"
     CDS             complement(1434..2171)
                     /locus_tag="VGR29_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2073540.1"
                     /translation="MTITFLTIRQFLRSRSLLVVVAVSLLPILFALIPHVVSEPFAIR
                     ELRETMADIIYLPFTATTLLPLATLVLATSALGDEIDDKTLHYLALKPVSRFRIVFEK
                     WLAVMIVTLPIIWIGIMATWLVLSWGHIDDMTDMVWPLLASALVGVLGFGSIFMLLSL
                     YIQRALLFGVFYVFIWESTLSRFLPGIRSISISHYTRSLFVRLLDDRRIVIDGPSGTT
                     TIVITIACIVIASLLLSTWRLRTMAME"
     gene            complement(2202..3146)
                     /locus_tag="VGR29_07845"
     CDS             complement(2202..3146)
                     /locus_tag="VGR29_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2073541.1"
                     /translation="MVRPGLAGDAEATVALDQVSKWFGDIVAVSDVSFGVGPGVTGLL
                     GPNGAGKSTALKMMAGLLPPSSGDVTVMGQPARGRPESYRELGLVSEQEMIYPFLSGR
                     EFVSLNARLQKVPNVGEATERAIDLVNMHDAADRRVGGYSKGMRQRIKIAGALVHDPD
                     VILMDEPLNGTDPVQRAEIIQLIRRLGRAGKTVVVSSHVLVEVERFAQNIIVIVNGKL
                     AAAGDYRAIRDQIDSHDHEIRIRASDVRELASYLVMQPMVRAVRLDRQERLVVETNDV
                     RSFYQIVPAAAKRADVRLYEVQSADDSLTSVFSYLVEQ"
     gene            complement(3214..4125)
                     /locus_tag="VGR29_07850"
     CDS             complement(3214..4125)
                     /locus_tag="VGR29_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073542.1"
                     /translation="MSTNPVHTTPEAPQRYGEIFDRGYKHYDGPRRGRSGAITSLIGY
                     SMKRAMGIRKSWTAKVLPFLLYIAVTIPLIVMIGLTAIAPDLEFASYSGYVSGIFFIV
                     GIFVGTAAPEMICVDRHERTLPLYFSRAIDRVDYVVAKVIAMSLLTMTMTVIPSVLLW
                     LGRQLTAEGIGQAMRDNIGDLGRVVFVGVMIALVLSTLGLAISSFTNRKGVAITVIII
                     SFLVLTGLANAGLELLSEMEWSRYLIALHLPLLFVGLSSHLFGDVNDRVVEQVDFSFA
                     AYMAYLIGLILLSLVILRWRYAPRDDT"
     gene            complement(4115..>4527)
                     /locus_tag="VGR29_07855"
     CDS             complement(4115..>4527)
                     /locus_tag="VGR29_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073543.1"
                     /translation="HKTMGISVVFSSHILEDIERVCDYVVILDGGRLITSQPLGDVNA
                     LTGEVALQLESGADQFAEALAAQGVQARRFESETSSYDQLLIKVEDERVYDLVRDIAV
                     SQRSVIRGMRSQARSLEDIYLGNVGAAREVNRVD"
BASE COUNT          960 a         1383 c         1351 g          833 t
ORIGIN      
        1 gtgcgcggca cgccgatcat gcgcgcaatc cttggtcatc aggaaggcac gagcgcggta
       61 tccgccctgt gccaacgcct tgcccatccg acgctcactc tcccgctcgt tgtactccca
      121 ggcattgtcg agaaaggtga cgccagcatc gatcgcctcg tgcacgatgc ggatggcttc
      181 atcttcggat ggtttgccga tgtgggcgcc gccgagtccg aggatcgata cctggtcacc
      241 ggtcccgcca aacggccggg ttgggattgc catggctagc tcctgtggga tgcgaatatg
      301 cggccgatca tcgcatggcc gacgcccatc gccaagcccc ggcgatcaca tggcacagcc
      361 gtcgtggatc ctcttgggct cgcgtcagac caggcggggt acgagcacgg atttgaactg
      421 cagcttacag cgatttgcag gaaggttgag catgtggccc atcctgcagg ggagcaccca
      481 acaggcccat ccgcagggtg ggagcgggag cgtcccattg tgtactccct tgtcaggggg
      541 cgggaggaca cggggtcctc ccctacgcag gcgtgcggtc aaaggtcatg caaacggctg
      601 taatccccgc tgaaacgacc ggacggaacg gcgtcgatat tgacggcgtc ccgtacagca
      661 atcggcatta ttcatttcgc tgcttgcaga ggcaaaacgg gtgtcccgcc ggatcgagca
      721 ggacatggaa actctcctgc ccgtcatcca ccagcttcgc gccgagttcg atggcgtagg
      781 cctgcgcttc ctcgatgtcc cgcaccgtga aatcgaagtg catctgctga ccgcgttcct
      841 gcgtcggcca cgtcggcgcc tgatagtcat ccaccttctg gaaccagatc tcacggccct
      901 cgggcgtgag aacggcgtcc tcgccgttca tctccaggcc gagcagcctg gaatagaacg
      961 tcgccagttc aagcgggttc ggacaatcga tcgccagcga accaagtttg acctcaacca
     1021 tgtcatgctc cttcgtttcg aacgccaacg gcgagtgcgg acagtccgaa cgccactggc
     1081 cctgaccgga ttctgaagat gcgagtgtgg ccaacgcgct tcgcgtagga ttcttcgccc
     1141 ttcgggctct gaatgacgag gagggggtgc cgtacgggta ggctgccatc tcacgtgttg
     1201 gggcagaggg ccttgaccgc gccctcgggg tttgccaaac aggtggtgag cgcggtcaag
     1261 ccctccccta cggtcgcctc cccccattgg atgcctgagg cgccgccgca ggggttatcg
     1321 tagacttttt cacacaccct ccagagaatc tgcttcgaaa acgagcatgt atcgcattca
     1381 gcaatacacg ctctattgtt acgtgcaggg cggccagttt gtgtccgctt ttgctactcc
     1441 atcgccatgg tgcggaggcg ccaggtcgag aggagcaggg aggcgatgac gatgcacgcg
     1501 atcgtgatga cgattgtggt ggtccctgag ggcccgtcga tcacgatgcg ccggtcgtct
     1561 agcagccgca caaagaggga acgggtgtag tggctgatgc tgatcgagcg gatgcccggc
     1621 agaaatcgcg aaagggtgct ctcccagata aagacataga agacgccgaa gagcagtgct
     1681 cgctggatgt agaggctgag cagcataaag atcgagccaa agccgagcac tccgacaagc
     1741 gccgaagcca gcaacggcca gaccatatcg gtcatgtcat cgatatgtcc ccatgacagg
     1801 accaaccatg tggccatgat accgatccag atgatcggca gggtgacgat catcaccgcc
     1861 agccacttct cgaagacgat gcggaaccgg ctgaccggct tcaatgccag gtaatgcagc
     1921 gtcttgtcgt cgatctcgtc accgagcgca gatgttgcca ggaccagggt tgccagcggc
     1981 agcagggtcg tggcggtgaa cggcaggtag atgatgtccg ccatcgtctc ccgcagctcg
     2041 cgaatggcga atggctccga caccacatgc gggatcagcg cgaagaggat cggcaacagg
     2101 ctgaccgcca cgacgacaag cagtgatcgc gatcgcaaga actgccggat ggtgagaaac
     2161 gtaatggtca agttctggct ctcgctttcg actccacggt gctactgctc gacaaggtag
     2221 gagaagaccg acgtcaggga atcatcggct gactgcacct cgtagaggcg aacatcagca
     2281 cgcttggccg ctgctgggac gatctgatag aaggacctga cgtcgttcgt ctcgaccacc
     2341 agcctctcct gtcgatcgag cctgaccgcc cgaaccattg gctgcatcac caggtaggaa
     2401 gcaagctcac gcacgtccga ggcgcggatc cggatctcat gatcatggga atcgatctgg
     2461 tcccggatcg cgcggtagtc gcccgccgcc gccaacttcc cattgacgat gacgatgatg
     2521 ttctgggcga atcgctcgac ctcgaccagc acatgcgacg agacgaccac tgtcttgccg
     2581 gcacgaccga gccgccggat gagctggatg atctcggcgc gttgcaccgg gtctgtgccg
     2641 ttcagcggct cgtccatcag gatcacgtcc ggatcgtgca cgagggcgcc tgcgatcttg
     2701 attcgctgcc gcatgccttt ggagtaccca ccaacgcgtc gatccgccgc gtcatgcatg
     2761 ttcaccaaat cgatagctcg ctcggtggct tcccccacgt tgggcacctt ttgtagcctg
     2821 gcattgaggc tcacgaactc ccgaccactg aggaagggat agatcatctc ctgctcggac
     2881 acgagcccca gctcccggta agattccggc cggccacgcg caggttgtcc catcaccgtg
     2941 acgtcgccgg aggatggcgg aagcaagcct gccatcatct tgagcgccgt tgacttcccc
     3001 gcgccgttcg ggccgagcag gccggttact cccggtccga caccgaagga aacatcagag
     3061 acagcaacga tatctccgaa ccactttgag acctgatcga gcgccacggt cgcctcggcg
     3121 tctcccgcaa gccccgggcg aaccatcctc acgggaggct ccacggtcgc aactggaata
     3181 tcgcgcgact ttcgaaatgc cacagctcgc cccttatgtg tcgtcgcgag gcgcatagcg
     3241 ccagcggagg atgaccaggc tcaggagtat cagcccaatc agataggcca tgtaggcagc
     3301 gaacgagaaa tctacctgct ccacgaccct gtcatttaca tctccaaaga gatgagagct
     3361 caagccgaca aagagcaacg gaaggtgcag cgcgatgaga tagcggctcc attccatctc
     3421 gctcagcagc tccaacccgg cattggccag accagtgagg accaggaagc tgatgataat
     3481 gaccgtgatc gccacaccct tgcggttcgt gaaggacgag atagcgaggc caagcgtgct
     3541 gaggaccagc gcgatcatga caccgacgaa aacgacccga ccgagatcgc caatgttgtc
     3601 ccgcatggct tgaccgatgc cttcggccgt cagctgtcgt cccagccaca gcaggaccga
     3661 cggaatcacc gtcatcgtca tcgtgagcaa ggacatcgca atgacctttg cgaccacgta
     3721 atccacacgg tcgatcgcgc gcgaaaaata gagtggaagc gtgcgttcgt gccgatcgac
     3781 acagatcatc tccggtgcgg cggtgccaac gaagattccc acgataaaaa agatgccgga
     3841 gacatagccg gaataggacg cgaactcgag atcgggtgcg atcgctgtca agccgatcat
     3901 gacgatcaac gggatcgtga cggcgatgta gaggagaaat ggaaggactt tggccgtcca
     3961 gctcttgcga atgcccatcg cccgtttcat cgagtagccg atgagcgagg tgattgctcc
     4021 gctccggcca cgccgtgggc catcgtagtg cttgtacccg cgatcaaaga tctcaccgta
     4081 tcgctgcggt gcctccggtg tcgtgtgtac aggattagtc gacacggttc acctcccggg
     4141 cggccccgac gttaccgaga tagatgtcct caagcgatcg tgcctgggaa cgcatgccac
     4201 ggatcactga gcgctgggaa acggcaatat cacgaacgag gtcatacacc cgttcgtcct
     4261 cgaccttgat cagcagctgg tcataggagc tggtttcgga ttcgaatcgc ctggcctgga
     4321 cgccttgcgc cgccagggct tcggcgaact gatcggcacc gctttcgagc tgcagcgcaa
     4381 cttcaccggt cagggcattg acatccccga gcggctgtga ggtgatcagg cgcccgccat
     4441 ccagaatgac gacgtagtcg cagacacgct cgatatcctc aaggatatgg gaggagaaga
     4501 ccaccgaaat ccccatcgtc ttgtgga
//
LOCUS       DASYWM010000189        13780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_495580, whole genome shotgun sequence.
ACCESSION   DASYWM010000189 DASYWM010000000
VERSION     DASYWM010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495580"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VGR29_07860"
     CDS             <1..1302
                     /locus_tag="VGR29_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV2073544.1"
                     /translation="TVYQHLITEYESQAPGAQVIAQYTPGKEDYGRKITTSFSGNTAP
                     DVFLINYREYGQYAASGALEPLGPRLRESPVISESDFYELPLDAFRGADGELVCIPQN
                     ISSLVVYYNVELFAAAGLDLPTADWTLDDFVEAAQALTLDVDNDGISDVHGLVTDSSM
                     VRYAPFIWMHGGEIVDNIEHPTRLLLDSPEARQGIEWFMDLGAGGHKVVPTEAETLAE
                     DNLSRFMNGRAAMMLDSRRVVPTLREIDAFVWDVAALPRGEERASVLHSDAFCMSAEV
                     ADKEAAWRFIEYAVGLQGQEILAATGRTVPSLRSVAESTVFYGDPDRPGAVGETVNTA
                     SPASAHVFLDTIPTLRRLPSFATWPEVEDIFNAEFQKSLYDDFDIEKALENVNSNAHD
                     AIRRATDDDSSKSVVDPLWAWHEGTPQRSVGPKERQSRSGY"
     gene            1487..3097
                     /locus_tag="VGR29_07865"
     CDS             1487..3097
                     /locus_tag="VGR29_07865"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEV2073545.1"
                     /translation="MDVIPFPRSTTATADVSCTITGSSAVVTGERVLQPIAEWLATEA
                     ERLASIPLSATSGTGPPIELALDDRLTSGVTTSGVRADGAALDCERYRLDVFKGSIRI
                     VGATPEGVFRGATTLIHLLAQAAVDGLATLVGVSITDAPRFAWRGLSLDVVRTFHPVD
                     TVKKVIDLLALYKLNVLHLHLTDSEGWRFEVPAYPNLTAIGGQTARDGRSGGYYTHSD
                     YAEILAHAGARFVTVVPEFDSPGHTASVLRAYPDLGSPEMHAAPDAMQYLDPSMPGVW
                     DLVRAVYDEMARVHPNGRMHVGGDEAIAMDEATFTHYMETALPAARSTGKGIVAWQET
                     ARAGFTDGDLMQFWISPHLVERVRLAAEDPDNSWLVQAFPDPAVRDAFVNLFLQAPDD
                     LPKALEQGADVVISRADKLYLDTRYVEPSADPAQEDARARLGLPQAVYGSGTIEDAFA
                     WDPATIQPELPLGRIAGVEGAIWCETITDERDLMFQLLPRLAGVAEKGWSDTREWTDY
                     HPRLGAQRQLWDAMGVSSFVSSVVWPEG"
     gene            3935..4966
                     /locus_tag="VGR29_07870"
     CDS             3935..4966
                     /locus_tag="VGR29_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2073546.1"
                     /translation="MISSFKVGISGGGTIGHIHAKALALIDNTKMVAIAEPREGAGRS
                     FAEEHGGTWYASLDDMLNAADIDVVIVATPSGLHPDQVARSARAGKHVITEKPMAITA
                     DGASSMIEACADAGVHFAVIFQNRLSADVMKVRRAIEKGYLGRPVLANGAMYWFRGPD
                     YYAANGGWRGTWEFDGGGALMNQAIHTVDLLQWLMGGITSIQAHTTTLTHEIETEDVA
                     SASFRFANGALGSITATTSAAKSYPVRIEIIGSEGRATLENNVVTEWDAEQPLDDNLL
                     TAEDRELVDGWQPNEPFGAAHTRQLRTIFRRLADGVQPPIAGAEARKALDIILGVYES
                     NRSGTQSRP"
     gene            4993..5847
                     /locus_tag="VGR29_07875"
     CDS             4993..5847
                     /locus_tag="VGR29_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV2073547.1"
                     /translation="MKYAVFTVSAPSMTPEEVAHKLKELGYDGIEWRVIDEPPNPAGE
                     GFWHGNKATVPLRGFVENAAEIRKLAEDNELEMPALGTYVRASSLEDVETAMKGAVAL
                     GVKRLRVNVARYDGAEPFMPLWNADREQYKRVVELAEKHGVQALIELHHGSICPSASA
                     GRMYVEGMDPAHVGVIHDAGNMVHEGFENYRMGLEVLGEYLAHVHVKNARWAPVEHSD
                     DQSVMWEASWAPLHKGVADIQQLFKALRAVGYDGWITVEDFSTERPLADRLQENLAFL
                     KKVEAETI"
     gene            complement(7269..8582)
                     /locus_tag="VGR29_07880"
     CDS             complement(7269..8582)
                     /locus_tag="VGR29_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEV2073548.1"
                     /translation="MKITDMIVTPVAIADAPLRNSVGIHEPYVNRIVLELVGENGLSG
                     FGEASYSTRTFEDLVALRPDVIGLDTANLNAILKVVDEHLGSPDENNLDPVFKNFDLT
                     PRLTNSAVARTYSPIEVAALDLSGRAWGVPVSELLGGRTRDAVPFSAYLFYKHAGGGG
                     EGIDDVRKDLYGEAMTPDAIVAQARQMIDAYGFRSIKLKGGVHPPDVEIATIGALRDV
                     FGPDVPLRIDPNCAWTVETSVYVGTELATTLEYFEDPTPGINGMSAVRISLLERGINM
                     PLATNMCVTGFNDVPESIAKDAVQVILGDHHYWGGLRAVVELSRICSTFGIGISMHSN
                     SHLGISLMAMAHLAAAIPTMTYDADTHYPWLHEDDEITSGGKIGFVDGCVIVPMTPGL
                     GFEIDRDALQRGHERFQRCSYRDRDDVVYMQRTVDPNWEKLLPRW"
     gene            9365..11272
                     /locus_tag="VGR29_07885"
     CDS             9365..11272
                     /locus_tag="VGR29_07885"
                     /inference="COORDINATES: protein motif:HMM:NF021534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEV2073549.1"
                     /translation="MKADSLRLHDIWAMLVAGWADRLDPTGANLICDGVRNDREAFGS
                     YEGTTRMMWGLGGWLSCPARPSRVRFRNQEYDIRELTQQALLAGTNPDSRGYWGDPES
                     SGTTQPTVESAQVAYAIWQSRRTIWDSLPANQQDQIARWLRLCGRTSHYEWKNNWALF
                     WALNHTVRKALGLDFDQGIIIDVMYRYLDDVYCGNGWYDDGATRGANRFDDYNFWVFA
                     SHVLAWSEVDCNADPERVAELLDRVRQLMAHYPYFFAADGAYPEYGRSGAYKFARLGA
                     PIWAYRHGIWPHSVGLLKTIVYRHMMWYVSHGAIRADGTLRQSLTSTGSNAIREPYIS
                     TGAPYWAMQAFGGLWSLPDDDPFWSAKQEPLPIERESFSIVLPQPGWIVTGSKASGHV
                     HRYPAHGRCHASQKYAKFVYSTAAPYNAARAEGMFLPDAMIGIAHGASITHREAARSP
                     ELHEDGWIRYGHDHQFGAQTATFETIIIPDGDLQLRIHRLVAAFPDQQIATIEGAAAL
                     GYDDGEELLTVFEPGNRISAAAASNRVVGIRSWDARSAKHAKPFGEAGSGNIVFGRSV
                     IPHVAGSISINEYAIATVFLGNIRQVSSHEVSTLLSDSPHLDTIDPVRLLVNWRGRSW
                     SIPVSDSDIRP"
     gene            11380..13260
                     /locus_tag="VGR29_07890"
     CDS             11380..13260
                     /locus_tag="VGR29_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEV2073550.1"
                     /translation="MKLAERFTIDTVRDALVAPGHWHPYPTIADRRDWEGLSAEQKNF
                     LIEEGEALLGTSWPELKATLFMEFQRNGNRSRYQAESYARRKMLIRLVMAECVENQGR
                     FLDDIVNGIWAICEESFWDVPAHNRYTRYPESALPDTEFPIVALFSAETGSVLAFADY
                     LLGEHLENHVPIVKERVQRELRDRLLDPYMERDDWNWLGFDRPPGSRAPNNWNPWIHS
                     NLLTIELMIEQDEEQRAKFVHRALNGLDQFLSGYHEDGGCDEGISYWGRAGASIYECL
                     DVLDTASRGRIAVWDEPLIKEIARYVLRTHIGGDWYVNFADGTAKTIPDPNVVYHYGE
                     RIEDEQLQTHAAASQADLTNEHTEFRSFNRTLNPYFRPIPQPETPPTYPLIKQSWLDG
                     IQVLTAREAEGSSQGLFLAAKGGHNDESHNHNDVGSFIVGLDGQPVLIDAGVEEYTKK
                     TFSDRRYEIWTMQSAYHNLPTVNGVQQAPGAEYHATDVSADIADDRAVLHLDIASAWP
                     QEAGVQRWERTVQLLRGQDAKVTISDDYMLTSDPQSITLTLMSASHVDASTPGQLRLE
                     GPKRALLVDYDVDIWQVETERIPVNDQRMSPVWGDCVTRILLHAKARTRSGSFSVTAH
                     AG"
     gene            13557..>13780
                     /locus_tag="VGR29_07895"
     CDS             13557..>13780
                     /locus_tag="VGR29_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073551.1"
                     /translation="MSEKTSRMKQRHSRRRFLQVAGATGFLVTASGRGDYAVAQTPVT
                     IDSFSQAPSLDAQDLPPVAERVGEMPLVLQP"
BASE COUNT         2941 a         4053 c         4038 g         2748 t
ORIGIN      
        1 accgtatacc aacacctgat tactgaatat gaaagccaag cgcccggggc ccaggtgatc
       61 gctcagtaca ctcctggcaa agaggactac gggaggaaga tcaccacttc gttcagtggc
      121 aacacagctc ccgacgtttt cctcatcaat taccgagagt acggtcagta cgcagcctca
      181 ggggcgctcg aaccgttagg tccccgtctc cgggaaagcc cagtgatctc cgagagtgac
      241 ttctatgaat tgccgctgga tgcatttcgt ggcgctgacg gcgagcttgt ctgcatcccc
      301 cagaacatct ctagcttggt ggtctactac aatgtcgagc ttttcgcggc tgcaggtctc
      361 gatcttccta ctgccgattg gacactggac gatttcgttg aggcggccca ggcattgact
      421 ctcgacgttg acaacgacgg catttctgat gtccatgggc tggtcaccga ctcttccatg
      481 gtccgatacg caccatttat ctggatgcat gggggcgaga tcgtcgacaa catcgaacac
      541 ccaacgcgcc tgctccttga ttcaccagag gcaaggcaag gcatcgagtg gttcatggat
      601 ctcggtgcgg ggggacacaa ggttgtcccg acagaggcgg agaccttggc ggaagacaat
      661 ctaagccgat tcatgaatgg acgagcggcg atgatgctgg acagccgccg agtggtaccc
      721 accctgcgcg agatcgacgc ttttgtctgg gatgttgctg cactaccacg gggagaggaa
      781 cgcgccagcg tacttcacag cgacgcgttt tgcatgtcgg cagaggtagc agacaaggag
      841 gcagcatggc ggtttatcga gtacgcagtg ggtctccaag ggcaggaaat cctggcagcg
      901 acgggaagga cagttccgtc actcagatca gttgccgagt cgacggtatt ctatggagat
      961 ccagatcgac cgggagcggt aggggaaacc gtgaatacag cttctcctgc cagcgcccat
     1021 gtcttcctcg ataccatccc aacgttgcga cgcttgcctt cgtttgccac gtggcccgaa
     1081 gtcgaggaca tcttcaatgc tgagttccag aaatctcttt acgacgactt cgacatcgag
     1141 aaggccctgg aaaacgtgaa ctcgaatgcc cacgatgcta tccgcagagc caccgatgac
     1201 gattcgtcca agtccgttgt ggatccattg tgggcatggc atgaaggcac ccctcagaga
     1261 tcggtcgggc ccaaggaaag acaatcaaga agtgggtact gatccgcgcc gtccctccgt
     1321 tctgagacct atgcagctgc gggaagtcgt catgtcggga agcggctctg gtggatccag
     1381 cgatgtaacg gcatgggaac tatccagacg acacctcctc ggaagtgtga gtggttcact
     1441 gcttgcctgg cggctgggca cagcaacggc cgcggaagga tcttcgatgg acgtcatccc
     1501 gtttccccga tcgacgaccg cgactgccga tgtaagctgc acgattaccg gctcgtcggc
     1561 cgtggtgaca ggggagcgcg tgctccaacc aattgccgag tggcttgcca cagaggccga
     1621 acgactggca tcaatcccgt tatccgcgac gagtggaacg ggtcctccca tcgaactcgc
     1681 gctggatgat cgattgacat ccggtgtgac gacctcaggc gtacgcgcag atggagccgc
     1741 tcttgattgc gagcggtacc gtctggatgt cttcaagggg agtatccgga tcgtgggtgc
     1801 cacgccagaa ggggtgttcc gaggcgcgac caccctgatc cacctcctgg cgcaggctgc
     1861 ggtggatggc ctcgcgaccc tggtcggggt gtccatcact gatgcgcctc gttttgcctg
     1921 gcgcgggctg agcctggacg tcgttcggac gttccatccc gtcgatacgg tgaagaaggt
     1981 catcgacctg ctggccctgt acaagttgaa cgtgctgcac ctccacctta cggattcgga
     2041 ggggtggcgg ttcgaagtgc cggcgtatcc gaacctgacc gccatcggcg ggcaaacggc
     2101 gagggacggt cgctcaggcg gctactacac gcacagtgac tacgccgaaa tcctcgcgca
     2161 cgcgggggcg cggttcgtca ccgtagtacc ggagttcgac tcgcctggtc acacggccag
     2221 cgtcctccgc gcctatccgg atctcggtag tccggagatg cacgccgctc ccgatgcgat
     2281 gcagtatctg gacccttcga tgcccggcgt ctgggacctg gtacgcgcgg tctacgacga
     2341 gatggcgcgt gtccatccca atggacggat gcacgtcggt ggcgacgagg ccattgccat
     2401 ggatgaagcg acgttcaccc actatatgga gactgcgctt ccagccgcca ggtccaccgg
     2461 caagggcatc gtggcctggc aggagacggc ccgggccgga ttcaccgacg gcgacctgat
     2521 gcagttctgg atcagcccgc acctggtgga acgcgtgcgc ctggcggcag aggatcccga
     2581 caactcgtgg ttggtgcagg ctttccccga cccggcggta cgggatgcct ttgtcaacct
     2641 cttcctgcag gcaccggacg atctcccgaa agcgctcgag cagggtgcgg acgtggtgat
     2701 ctcgcgtgcc gacaagctct acctcgatac gcggtacgtt gagccgtcag ccgatccagc
     2761 acaggaagat gctcgcgccc gccttggcct gccgcaggca gtctacggca gcgggactat
     2821 tgaggatgcc ttcgcctggg atcctgccac gatccagcca gagctcccgc tcggccggat
     2881 tgcaggcgtc gagggtgcga tctggtgcga gacgatcacc gacgaacggg acctgatgtt
     2941 ccaactcctg ccacggctgg ctggagttgc cgagaagggc tggagtgaca cccgggagtg
     3001 gaccgattac catccgcgcc ttggagccca gcgccaactt tgggacgcga tgggagtgag
     3061 ctccttcgtc tcgtccgtcg tgtggcccga ggggtagccg gtcgtctgtc acgggtggta
     3121 ggtgactcgg cacgtgcctg agcagaacta ctcgcgcgcc gtacaggaga ccggcgtgcc
     3181 ccatgtcgtt cggtgaccgt cgctcaatca ggatttctga cggtgccccc ccgggattcg
     3241 aagccggaac caaggattaa acgtctgcaa gagccgcctc catgggaatt catgcgagtc
     3301 tactctctcc atagaatggg tcgatttttg aggggttcag tccaccgctg tcctcaacga
     3361 tccgttccct ttggagtcag gttggcgtca aaacgaccgt ttcatgacga gttcttcacc
     3421 cgcgtcccgc ggaaaggggt gagtgatgcg cgataaaagg ttttggattc gccccggagg
     3481 actcttacgt cgacatccct gctccaatgc aagcgacatc tggctccagg cctgttgtga
     3541 ggaattcgac gaaacctcgg tccttctgtg gaatagcgtg cgcaagcagt ttggcacgca
     3601 gacctcgctc ttccaaagcc ctccactcta aagtctgtcg tgcttggccc ggtgccgcca
     3661 tggcaacagc gcagagggac gaaacatcat cgtgtagggg caagatccat aatgatcgct
     3721 cctgtacaca ctgatctcgc ttccgcacca caacttttgt gcatctgctt gggcagcatg
     3781 gcacagcgac attcttatct tatcgggatt cctgccaacc ccgttgccct acttccagat
     3841 ccgtattgtg gtcgccgcat tcgccggata atatgtgccc aagcgatcac aggagcgtca
     3901 gcattccatt tgttcatgga aggaagaccc tatcatgatt tcgtcattca aggtcggcat
     3961 ctctggaggt ggcacgatcg gacacatcca cgcgaaagcg ctggcgttga tcgacaacac
     4021 gaagatggtc gccattgcgg aaccgcgtga gggcgccggc cgatccttcg ctgaggagca
     4081 cggagggacg tggtatgcct ccttggatga catgctgaac gcggcagaca tcgatgtggt
     4141 catcgtggcg acgccctcgg gcctccaccc tgatcaggtt gcccgtagtg cgcgcgcggg
     4201 taagcacgtg attaccgaga aacccatggc gatcacggcc gatggtgcga gcagcatgat
     4261 cgaggcatgc gcggatgccg gggtacattt cgctgtcatc ttccaaaacc gcctttcagc
     4321 cgacgtcatg aaggtgcggc gcgctatcga gaagggctat ctcggcagac cggtgctcgc
     4381 gaacggcgct atgtactggt ttcgcggccc cgattactat gcggcgaacg gtggttggcg
     4441 tggcacttgg gaatttgatg gcggtggtgc cttgatgaac caggcgatcc atacggtgga
     4501 tctgcttcaa tggctcatgg gcggcatcac gtccatccag gcccatacca caacgctgac
     4561 tcacgagatc gaaaccgagg atgtcgcctc agcgagtttc cgctttgcca acggcgcgct
     4621 cggcagcatc acggcgacta cttccgcggc gaagagctac ccggtgcgca tcgagatcat
     4681 cggctccgaa ggccgggcga cgctggagaa caacgtcgtc actgagtggg atgcggaaca
     4741 accgcttgac gacaacctcc tgacggccga ggaccgggag ttggtcgacg ggtggcagcc
     4801 gaacgagccc ttcggggcag cgcatacccg ccaactgaga acaattttcc gacgactcgc
     4861 agacggtgtc cagccaccga ttgccggagc cgaggcacgc aaggcgcttg atataattct
     4921 gggtgtttac gaatcaaacc gctctggtac gcaaagccgc ccatagtact agtgcatgga
     4981 ggacaataca taatgaagta tgccgttttc actgtttccg ctccttccat gacgccggag
     5041 gaagtcgccc acaagctcaa agagcttggg tacgatggaa tcgaatggcg cgtcatcgat
     5101 gagcccccga atccggcggg ggaggggttt tggcatggca acaaggcgac agtcccgctc
     5161 cgcgggttcg tggagaatgc cgctgagatc cgtaaactcg ccgaggacaa cgaactcgag
     5221 atgccggctc ttggcaccta cgtcagggct tcttcgctgg aggatgtaga aacagcaatg
     5281 aaaggcgccg tcgctctcgg ggtgaagcgt ctccgtgtaa acgtcgcccg ctacgatgga
     5341 gccgagccat tcatgccctt atggaacgcg gaccgtgagc agtacaagcg agtagtcgaa
     5401 cttgcggaga agcacggcgt tcaggcgctg atcgaactcc atcacggatc catttgtccc
     5461 tcagccagcg ccggccgcat gtatgtggaa ggcatggacc cagcgcatgt gggcgttatc
     5521 cacgacgcgg gcaacatggt gcacgagggt tttgagaact accggatggg gctcgaggta
     5581 ctcggcgaat atctggctca tgttcacgtc aagaatgcaa ggtgggcccc cgtggagcac
     5641 tcggacgacc agagtgtgat gtgggaagcc agctgggccc ccctgcacaa gggagtcgct
     5701 gacatacagc aactcttcaa agcgctgagg gccgttggct atgacggctg gatcaccgtc
     5761 gaggacttct cgacagagcg gcccctggca gatcgtcttc aggaaaatct ggccttcctg
     5821 aaaaaggtcg aagcggaaac gatatagcca gcatcgatgc ccttgctcct gcgccagacg
     5881 cggcttggaa agagtccctc ctggagacgc tcttgtcgaa agatgaggca gcgtctggtg
     5941 ctgcgttcgg tcttgacgcc cggtgtaccc gaaggaaacg ctcggctatc tatcgcatcc
     6001 atccgcttaa ccagctaggg gatacgaggt cgatgaggcg gtcgatctca tggatgttca
     6061 ggttgcttcg cggtgaaggc gggagagctt gcccttcaag ctactccgga actatgagcg
     6121 gacctcgcaa ctaccgactg ttgcgcacga atgatcgctc cgggagcatc ctgatggaga
     6181 atgcagcatc aagtgacgaa cggtcattgc aggtcgagca cctggcggtt cggatatttt
     6241 ggaccaagcg tcagtcggcg cagccgcggc tacggacgtt gcgaccgcaa tgaccgagac
     6301 aatcatgatc ggcgacaggc gcggatgacc tttgctgccg caccatccca gaacgagttc
     6361 ctcgccgcgc tagccgcgga gtcagacctc ccctggtcgc gggtctcagt atttcagctc
     6421 gaagagtacg tcggcctgcc ggaagacgct caccagttct tcggcacctt cttgcgtttg
     6481 cggctcgtcg atacgccaga actccggttg caccggcacc accacacgcc gcgcgccgtc
     6541 ccaaccgaga gtacaagtcc gcctagattc accagaaggc agcgtttgca accgctgaat
     6601 gtcttcgccc cgtcatcacc acgccccctt ttgcgctggt gcgagtcgac agactgtcca
     6661 gcataccggc cgttggtgct gagggtcgtc ccacgcggtg ccgtagaacg cggtgctcac
     6721 gagctcgcgg tgagcatgtt tcggtaggcg accaccggat ctggctttga ggacaagcgg
     6781 tccgaggatc accgactgag cacggctcgg gtcggtcggg taagcgcggc aggacacggt
     6841 gtacacccgc aaagagagga gcctgcacgt atcctcctgg ttctacgctc ctttcggtcg
     6901 ccctctcagg cgacttgcgg gataggagac aagcatccgc gaagcgtccg gtttctttag
     6961 cgatttcagc tgccgctcat attgatgttg gtaatggggc ggaattcgct tgaacggtgt
     7021 acgcggctcg agtatttggt ggcgtctcaa acaaagggcg cccccatgta aacgccatga
     7081 actggtggcg aagagtctct agtggtggca aggccgccga aacgccacac ccctcctcgg
     7141 gctgaactca accacgctcc aacccacgca acgcgagcac caggttctca ccaatagagt
     7201 cagcataagt gcagccccgg gtacaggcga aatcgaacca aagcaatatc aactgcgcac
     7261 cactccaatt accaccgcgg gagcagcttt tcccagttcg ggtcaacagt acgctgcatg
     7321 tagacgacgt cgtcacgatc ccgataggaa catcgctgga agcgctcatg accacgctgc
     7381 agggcgtcac gatcgatctc aaagccaagt ccaggtgtca tgggaacgat cacgcaccca
     7441 tcgacgaatc cgatcttgcc ccccgaagtt atctcatcat cctcatgcaa ccaaggataa
     7501 tgcgtatccg catcgtaggt catcgtggga atcgcggcgg cgagatgtgc catcgccatc
     7561 aacgagatgc ccagatgtga gttggaatgc atactgatgc cgatcccgaa ggtcgagcaa
     7621 atgcgactaa gctcaacgac cgcgcgtaaa ccaccccagt aatggtgatc gccgaggatg
     7681 acctgaacag cgtccttggc aatcgattca gggacgtcgt tgaatccggt aacacacata
     7741 ttcgtagcca gcggcatgtt gataccgcgt tccagcaaac tgatgcggac ggccgacatg
     7801 ccgttgatcc ccggcgtcgg atcctcgaaa tactccagtg tcgtcgcgag ttctgtccct
     7861 acgtagaccg atgtttcgac ggtccaggcg caattggggt cgattcggag cgggacatcg
     7921 ggcccaaaga catcccggag cgccccaatc gtcgcgattt ccacgtccgg cgggtgaacg
     7981 ccacccttca gtttgatgct cctgaagccg taggcatcga tcatctgccg cgcctgcgct
     8041 acaatcgcat ccggcgtcat cgcttcgcca taaaggtcct tacggacgtc gtcaatccct
     8101 tccccgcctc cgccggcatg tttgtaaaaa agatacgcgc tgaacggcac agcatcccgc
     8161 gtacggcccc cgagcagctc acttaccgga acaccccagg cgcgtccaga gaggtcaagg
     8221 gcagccacct cgatcgggct gtatgtgcgt gcgacggcac tgttcgtgag gcgcggggtg
     8281 agatcgaagt tcttgaacac cggatcgagg ttgttctcat cgggcgaacc caggtgctca
     8341 tcgactacct tcagaatcgc attcagattc gccgtatcaa gtccgataac gtcagggcga
     8401 agggccacaa gatcctcgaa ggttctggtt gaatacgagg cttcgccgaa acctgagagc
     8461 ccgttctcgc cgacgagctc taatacaatt cgattcacat agggctcatg aatgccgacc
     8521 gaattgcgaa gcggtgcatc cgctatggct acgggagtca cgatcatgtc cgtaatcttc
     8581 actcacccac ctccgtgaaa aggtgttgca gacgtgtacc gcaggaggcg tctgccatcg
     8641 tgctgcaact agcatctcat cgtcttgcat tatgaactgg tatacgggct ctatctgata
     8701 gagcaacgac cgataaccgt gtttaacagg cgaacgattc cctcgccccc ttcatcggtc
     8761 taggcttcag aaccgttacg atattgccgc agatcgaaaa atccgacgaa tctctgctgc
     8821 catcgtagct gactggttgt tgcgcagggt gaaggagtgt agtaatggga gtggccggca
     8881 gaccggcgtt agtctccccg ttctccagcg ctggcaaccc aacgtcgggg agatccgagt
     8941 aacatccccg cgagaagagc gcgtcgttga tccgagcatt cggaatactg gccacgtgtc
     9001 gctcgggctg gaattgattc agaacacaat gaaccgggat ggcacaaatg ttgagtttac
     9061 ggacaacctc accaatgacg acctgctagc cagccgagat ttgacatcgc gtgatcactg
     9121 agaggagcgc ctcaccaacc cattctgcct tggtgcggtg acattaaccg ttcttttgag
     9181 ccaattgggc taaggcgatc agcccccttg agaccgcacc gatagcggcc tatcacagca
     9241 acatgttttg ccataccggc gcgggcgatc tcgcgggcag ccatgcttca gcccgggatc
     9301 cgtatggtat atgttggtct aatcgctccg caccatcgtc catctttatt gcgaggatac
     9361 agctgtgaag gctgattcgc ttcgactcca cgatatctgg gcaatgctag tcgccgggtg
     9421 ggccgatcga ctcgatccga cgggcgccaa cctgatctgc gatggcgtgc gcaatgacag
     9481 ggaggcgttt ggttcctacg aaggcaccac acggatgatg tgggggttgg gggggtggct
     9541 ttcatgtccg gcccgcccat cgcgggttcg attccggaat caagagtacg acatccgtga
     9601 actgacccag caagccctgc tcgcggggac gaaccccgat tcacgtggat actggggtga
     9661 tccagagagc agtggcacca cccaacccac cgtcgagtct gcacaggtgg cctacgcaat
     9721 atggcagtcg cgtcgaacca tttgggattc cttaccagca aatcaacaag atcagatcgc
     9781 gaggtggctg cgtctttgcg ggcgaacatc tcattatgaa tggaaaaaca actgggcgct
     9841 cttctgggcg cttaaccaca ccgtccgaaa ggcactgggg ctggacttcg atcaagggat
     9901 catcatcgac gtgatgtacc ggtatctcga tgacgtgtat tgtggcaatg gctggtacga
     9961 tgacggagcg acacgagggg ctaatcgctt tgatgactac aacttttggg tgtttgcgtc
    10021 gcacgtttta gcttggtcag aggttgattg taacgctgat ccggagcgcg tggctgagct
    10081 cctcgatcgg gttcggcagt taatggctca ttacccttat ttcttcgccg ccgacggcgc
    10141 ctacccagaa tacggccgaa gcggagcgta caagttcgcg cgcttgggcg ccccgatttg
    10201 ggcatatcgt cacggcatct ggccgcatag cgttggtttg ctcaagacaa tcgtctatcg
    10261 ccacatgatg tggtacgtct cgcatggggc gatccgagcg gatggcacac ttcgccaatc
    10321 actgacatcg accggctcaa acgcaatccg ggagccatac atctctaccg gcgccccgta
    10381 ttgggcaatg caggcattcg gaggattgtg gagtctccct gatgatgatc cattctggag
    10441 tgcaaagcag gaaccgctcc ccatagagcg agagagcttc tctattgtgc tgccacagcc
    10501 cggctggatt gtcaccggaa gcaaagcatc cgggcatgtt caccgctatc cggcccatgg
    10561 ccgctgtcac gcctcccaaa aatacgcaaa attcgtctat tccaccgccg cgccgtataa
    10621 cgcggcacga gcagaaggca tgttcttacc cgacgcaatg atcgggattg cgcacggagc
    10681 gagcatcacg caccgagagg cagcgcgttc accggaactt cacgaagatg gctggattcg
    10741 ctatggacac gaccaccaat ttggtgctca gactgccacc ttcgaaacca tcataatccc
    10801 ggacggggat ctccaattgc gcatccatcg gctcgttgcg gccttcccgg accagcagat
    10861 tgcaaccatc gaaggggcag cggctctcgg ttacgacgac ggcgaagaac tgcttacggt
    10921 cttcgagcct ggtaaccgga tcagtgcggc ggcggcctcg aatcgagttg tcggcatccg
    10981 cagctgggat gcccgatcag cgaagcatgc gaagccgttc ggagaagcag gctccggcaa
    11041 catagtgttc ggacgaagcg ttatccctca tgtggccggc agcatctcca tcaatgagta
    11101 tgcaattgcg acagtcttcc taggcaatat ccgccaggta tctagccacg aggtatcgac
    11161 actgctcagc gatagtccgc acctcgatac gatcgatcct gtcagacttc tagtgaactg
    11221 gcgcggtcga tcctggtcga ttccggtcag cgacagtgac atacgtccgt agaaggcgtt
    11281 tacaggggat ctcatatgac gtacggtcct tgaagtcatg gaaacggcag tatagcccag
    11341 cgcttcttcc ttcacgggtg tcggctggga ggaggtccca tgaaactcgc agaacgattt
    11401 acgatcgata ccgtccgtga cgccctcgtt gctccgggac actggcaccc gtatcccaca
    11461 attgccgatc ggagggattg ggaagggctc tcggctgagc agaagaactt cctgattgag
    11521 gaaggggaag cgctgctcgg cacgtcatgg ccggaactca aggcgacgct attcatggag
    11581 ttccagcgga atggcaatcg ttcccgctat caggctgagt catatgcccg acggaagatg
    11641 ttaatccggc ttgtcatggc cgaatgtgtc gaaaaccagg gccgtttcct ggacgatatc
    11701 gtcaatggca tttgggcgat ctgtgaggaa agcttctggg atgttcccgc acacaatcgt
    11761 tacacacgtt acccggaatc ggcactcccg gataccgaat ttccgatcgt cgcgttgttc
    11821 tcggccgaaa cggggtcggt gctcgctttt gcagactacc tgctcggcga gcacctggaa
    11881 aatcatgttc ccatcgtcaa agagcgcgtt caacgggagc tccgcgacag actgctggat
    11941 ccctatatgg agcgcgatga ctggaactgg ttggggttcg accgaccacc tggcagccgc
    12001 gccccgaaca actggaaccc ttggattcac agcaatctac tcaccattga actgatgatc
    12061 gagcaggacg aagagcaacg ggccaaattt gtgcaccgcg cgctgaacgg tctcgatcaa
    12121 ttcctcagtg gttaccatga ggatggcgga tgtgacgaag gtatttcgta ctggggtcgc
    12181 gccggtgcca gcatctacga atgtctggac gtgctcgaca ccgcctcgag aggcaggatt
    12241 gcggtatggg atgagccgct aatcaaggaa atagcgcgct acgtcctccg aacgcatatc
    12301 ggtggggact ggtacgtcaa cttcgcagac ggtaccgcca agaccattcc tgacccgaac
    12361 gttgtctatc actatggtga gcgaatcgag gacgagcaac tccagaccca tgccgccgcg
    12421 tcacaggctg atctcacgaa tgaacatact gaattccggt cgttcaatcg caccctcaat
    12481 ccctacttcc gcccaatacc acaaccagaa acaccgccca cctacccgct gatcaaacag
    12541 tcatggctcg acggcatcca ggtgctgact gcacgggaag ctgagggatc aagccaaggg
    12601 ctcttcctgg ccgccaaggg aggtcacaac gacgagagcc acaaccacaa cgacgtcggg
    12661 agcttcatcg tcggactcga cggccagccg gtactcatcg atgccggcgt ggaggagtac
    12721 acgaagaaaa cgttcagtga tcgacgatac gagatctgga cgatgcagag cgcctaccat
    12781 aacctgccca cggtgaacgg ggtccagcag gcgccaggtg ccgagtacca cgctaccgat
    12841 gtatcggctg atatcgccga tgatcgcgcc gtgcttcacc tcgatattgc ctccgcgtgg
    12901 ccacaggagg ccggagtgca acggtgggag cgaacggttc aactcctccg cggccaggac
    12961 gcaaaggtga caatctcgga tgactacatg ctcaccagcg atccgcagag catcacgctc
    13021 acgctgatgt cagcatcaca cgttgatgca tccacaccgg ggcagttgcg tctggaaggg
    13081 ccgaagcgag cgttgcttgt cgactacgac gtcgacatat ggcaggtcga aaccgaacgg
    13141 atacccgtca acgaccagcg catgtcaccg gtgtggggag actgcgtcac gagaatcctt
    13201 ttgcacgcaa aagcaagaac gaggagcggg tcctttagcg tgacggccca tgccggctaa
    13261 ggcatggatc accgatctca gcccgtccag ccatatcagc acgttaccct cttcggccaa
    13321 gaactggaca ctggacagga aaaccgaaaa gagtgctttc gtgccgcacc ttgcacaggc
    13381 agggtagatg tcgtagcatg agcgcacgtg cacaatggaa tcgcgactcg tgagtctata
    13441 ccctggccgc aatgtctggt cgctgtcttt acaaaccaga gacttgtctc cggttccagg
    13501 ctgctacacc gtcgctcgct gcggatatcg catccgcaca tcaggaggac tcccttatgt
    13561 ccgagaagac ttcgcgcatg aagcagcgac actcccgccg ccgcttcctg caggtcgccg
    13621 gagccaccgg ctttctcgtc acagcctccg gtcgtggcga ctacgccgtc gcccagaccc
    13681 ccgtcaccat cgactccttc tcacaagccc cctcccttga tgctcaggac ctgccgcccg
    13741 tggccgagcg cgtcggtgag atgcccctgg tgctccagcc
//
LOCUS       DASYWM010000190         4666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_664637, whole genome shotgun sequence.
ACCESSION   DASYWM010000190 DASYWM010000000
VERSION     DASYWM010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664637"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..285)
                     /locus_tag="VGR29_07900"
     tRNA            complement(213..285)
                     /locus_tag="VGR29_07900"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Gly,seq:ccc)
     gene            complement(333..1130)
                     /locus_tag="VGR29_07905"
     CDS             complement(333..1130)
                     /locus_tag="VGR29_07905"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV2073552.1"
                     /translation="MAERNPVSLRVMTYNIRHGPGNDPEDPKLFDDYDLERVGEVIRH
                     VDPHIVALQEVDRLQERSLGVDQPQALGVLLEMERCFGANVLFKPGEYGVATLGTYPI
                     TSCENVSLPTGEGRENRGVLKASIHVPGYGSITVLNTHLQVGQPGSEGAAVEERREQA
                     SVIGDLVSNENGPVILMGDFNAEPGDPELAPLAFLSDPWRLAGEGGGGCTIPSHPALQ
                     PSLRIDAIYVNEGFQVISSRVVLDHPARFASDHLPVVANLEIAGWKG"
     gene            1257..1329
                     /locus_tag="VGR29_07910"
     tRNA            1257..1329
                     /locus_tag="VGR29_07910"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1290..1292,aa:Ala,seq:cgc)
     gene            complement(1388..2026)
                     /gene="hpt"
                     /locus_tag="VGR29_07915"
     CDS             complement(1388..2026)
                     /gene="hpt"
                     /locus_tag="VGR29_07915"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV2073553.1"
                     /translation="MDTSISSDDLQAIEVPDHKKARGVARVLISESEIRDAIHTMARE
                     LDEEYADDVPLVICVLRGAIAFMSDLVKQMSVPLEMDFMAISSYGSSTRSSGVVRILK
                     DLDEEVEGRRLLIVEDIVDSGLTLQYLLDYLERRNPRDVRVVALVKKDKPDAISVQVD
                     RVGFTVPDEFVVGYGLDYAGEYRNLPFIGVLDPSVYNGEGAQQAQSGDPAQT"
     gene            complement(2064..3149)
                     /gene="tilS"
                     /locus_tag="VGR29_07920"
     CDS             complement(2064..3149)
                     /gene="tilS"
                     /locus_tag="VGR29_07920"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV2073554.1"
                     /translation="MTYERTLSAARRESGLIQRVRASLLRGFPDPASPHILVACSGGR
                     DSVVLAHLVQQLVRMGLIRATIAHIDHALRPDSADAARMVQAFASRIGLPARVARLSA
                     DAVNRHPGMGLEEALRRERYLALAGIAGEIAADAVALAHHQGDQAETVLLHLVRGAGL
                     HGAAGMREWSLINVPWWVQSCGGVPLKLWRPLLPESGGQIASWHSDHNLPLYEDSTNQ
                     DLKIRRNAIRHIVLPHLEDIVPGATANLARFADLAAEDNEVLEGMAEERASVERDGSL
                     HRKVIVDAPRAIQRRIVRQWILGRMLDIELTANRIDAVCRLAKANRSGSRVEVGGGWA
                     VQIDRGVLSLVPALQAPCADGDVASAD"
     gene            complement(3175..3249)
                     /locus_tag="VGR29_07925"
     tRNA            complement(3175..3249)
                     /locus_tag="VGR29_07925"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3215..3217),aa:Met,seq:cat)
     gene            3452..>4666
                     /locus_tag="VGR29_07930"
     CDS             3452..>4666
                     /locus_tag="VGR29_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV2073555.1"
                     /translation="MTDLQSPLLAGLNPDQQQAVTTTEGAVLVVAGPGSGKTRVLTHR
                     IGYLIREQGVSPDQILAVTFTNKAAREMRDRIDRLIGEAATDGLVMGTFHSLGVRMLR
                     QNPLSVEAHLQLRRNFVIYDDGDQMEIARTAIRNAGLDPKQIVPRRMLSRISAAKSVL
                     MTPEEVANEAETYDDEIVVRVYKEYEKLLRGANAVDFDDLLGLPIRLFDHDPGVLERY
                     QQRFRYIMVDEYQDTNRVQYVLVSALADLHRNLFVVGDPDQSIYAWRQADIRNILDFE
                     KDYPAATQIHLEINYRSTQRIVEAADRVIRDNEQRIDRRIRTINEEGEKIQVRELSDQ
                     QHEAQFIVSEIRRMTARGVCRPEDVAVMYRTTAQSRALEEAFRTSEFRYRIVGGVRFY
                     ERKEVRDILAVLR"
BASE COUNT          936 a         1495 c         1348 g          887 t
ORIGIN      
        1 ggccaacatc ttctggtgcc ggacggtgtg aggttttggg ggtaatgggt acacggatgc
       61 ggttacgagt accgctcgta catcgcccag catggctcct aagagacccg gcggttttct
      121 ccggtacaca tttggtacac atttgcttac acacgagaaa cggcccgcca tggtgacgga
      181 ccgttttccc tgatttcatg ggcttttcgt agagcgggtg accggattcg aaccggcgac
      241 attttgcttg ggaagcaaac actctgccaa ctgagttaca cccgcatacg ctctcgcgcc
      301 atcgcattat atcatctgga aggagccgcg gatcagcctt tccaccccgc aatctcgagg
      361 ttcgccacca caggtaggtg gtcggaggcg aaccgtgccg ggtgatcgag aacgacccgt
      421 gacgagataa cctgaaaccc ctcgttcacg tagatcgcgt cgatacgcag ggagggctgg
      481 agcgcgggat gggaagggat ggtacagccg cccccgccct ctcctgcgag ccgccaggga
      541 tcgctcagga acgccagcgg cgctagctcc ggatcgcccg gctcggcgtt gaagtcgccc
      601 atcaggatca ccggcccatt ttcattgctg acgagatcgc caatgacgga tgcctgttct
      661 cgtctctcct cgacggcggc cccctcggag cccggttggc ccacctgcaa gtgcgtgttc
      721 aggacagtaa tgcttccata acccggaacg tgtattgacg ccttgagcac tcctcggttc
      781 tcccgccctt cccctgtagg cagggagaca ttctcgcaag aagtaatggg atacgtgccc
      841 agggtagcta cgccatattc gcccggcttg aacaggacgt ttgccccgaa gcaacgctcc
      901 atctccagca gtacgccgag ggcttggggc tgatccacgc cgagcgatcg ctcctgcagc
      961 cgatccacct cctggagcgc cacaatatgg ggatctacgt ggcggatgac ctcccctacc
     1021 cgctccaggt catagtcgtc gaaaagcttc gggtcctccg ggtcgttgcc cggcccatgc
     1081 cggatgttgt aggtcatcac gcgcaggctc accggattcc tttcagccat ttatggtccc
     1141 ttcctcgcat atggtcccat gggaccgtat gcgttcaacg ggctggaccg ccggtcgcac
     1201 cagttctcgt cacattgacc gcttcattcc cggtcgcgta tactcgcatt cgctctgggg
     1261 acgtagctca gttgggagag cgctacaatc gcactgtaga ggtcgggggt tcgagtcccc
     1321 ccgtctccac cccaggtaga cagacaaaag ccttcgcgat catgcgaagg cttttcgcta
     1381 tgcccggtta cgtctgggcc ggatcccccg actgagcctg ctgcgcgcct tcgccgttat
     1441 agaccgatgg gtcgagaaca ccgatgaacg gcaggttccg gtactcgccc gcgtagtcca
     1501 acccgtatcc caccacaaac tcgtcgggca ctgtgaagcc gactcgatcg acctggacgc
     1561 tgatcgcgtc aggcttgtct ttcttgacca gcgccacgac ccggacatcc ctcgggtttc
     1621 ggcgctccag atagtccagc agatactgaa gggtcaggcc gctatccacg atgtcctcga
     1681 cgatgagcag gcgccgaccc tccacttcct cgtctaggtc cttcaggatg cggacgacac
     1741 ccgaggacct cgtcgatgag ccgtagctgg agatcgccat aaagtccatc tccagtggca
     1801 ccgacatctg tttcaccaga tccgacatga aggcgatcgc ccccctcaac acgcagatga
     1861 caagcgggac gtcgtcggca tactcttcgt cgagttcccg cgccatggta tgtatcgcgt
     1921 cccgaatctc ggactccgaa ataagcacgc gagcgacacc ccgggccttc ttgtggtccg
     1981 gcacctcgat cgcctgaagg tcgtctgaag aaattgacgt atccatggaa tttgcccctg
     2041 tggtcatgcc tcttgacgct cgcttaatct gctgaagcca catccccgtc cgcgcatggt
     2101 gcctgcaggg caggaacgag cgatagtaca ccgcgatcaa tctgcacggc ccacccgccg
     2161 ccgacctcca cccgcgaccc gctccggttc gcctttgcca ggcgacagac agcatcgatt
     2221 cggttcgccg tcaattcaat atccagcatc cgccccaaaa tccactgtcg cacgatccgg
     2281 cgctggatcg ctcgaggggc gtcaacgata actttgcgat gcaggctgcc gtcgcgctca
     2341 accgatgcac gctcctcggc catgccttcg agcacctcat tgtcctcggc cgcgagatcg
     2401 gcgaatcggg caaggttggc ggttgcgccg ggaacaatat cctcgagatg gggaaggacg
     2461 atgtgacgta ttgcgttccg gcggatcttg aggtcttggt tggtactgtc ctcgtaaagg
     2521 ggaagattgt gatcactatg ccaggacgcg atctgacccc ccgattcagg cagcaggggg
     2581 cgccaaagtt ttagcggcac gccgccacag ctttgcaccc accatgggac gttgatgagc
     2641 gaccattccc gcatgccggc tgcaccatgc agtcccgcgc cccggacgag gtgcaacagc
     2701 acagtctcag cctgatcgcc ttgatgatgc gcgagcgcca cggcatcggc ggcgatttcg
     2761 ccagcgatcc cggccagtgc gaggtagcgc tcccggcgca gtgcctcctc gagccccatg
     2821 ccaggatgac gattcactgc atcagcgctc aaccgggcga cgcgagccgg caagccgatc
     2881 ctggaggcaa aagcttgcac cattcgtgcg gcatcggcgg aatccgggcg cagggcatgg
     2941 tcaatgtgcg cgatggtagc gcgtatcagg cccatcctga cgagttgctg gacgagatga
     3001 gcgagcacga ccgagtcccg cccgcccgag caggcaacca aaatgtgagg tgacgccggg
     3061 tcggggaaac cgcgcaacag tgaggcgcgc acccgctgaa tgaggccgga ctcccgccgc
     3121 gctgcgctca aggtccgctc atacgtcata tacatctgtc cgatcgaggg gaattggtgc
     3181 cgacgggtgg attcgaacca ccgaccgagg gcttatgagt ccctagctct gccactgagc
     3241 tacgtcgaca tttgacgcga aaatcctaac aaacgcccct acccatgtca aatgcagcac
     3301 cccctgatcg tgcggcgtcg cgacatccgg atgctacact caggaacgat agagggggtc
     3361 gtccgatccc cggacaccat ccattctcac cgcgttcatg tgccgttccg ggcctcggcc
     3421 tgccaccacc tgaatggaaa tcattcacgc tatgacggac cttcaatctc ctctccttgc
     3481 cggcctcaat cccgaccagc agcaagccgt cacgaccaca gaaggcgccg tcctggtggt
     3541 tgcgggtcca ggttccggca agacgcgagt gctcacccac cgcatcgggt acctgattcg
     3601 tgagcaggga gtcagcccgg atcagatcct cgcagtcacc tttacgaaca aggccgcgcg
     3661 ggagatgcgg gaccgtatcg accggctcat cggtgaggcc gccacagatg gtcttgtcat
     3721 ggggaccttc cactcgctcg gtgtaaggat gctgcgtcag aacccgctga gtgtcgaggc
     3781 acacttgcag ctgcggcgaa acttcgtcat ctacgacgac ggggaccaga tggagatcgc
     3841 aaggacggcg atccggaatg ctggccttga ccccaagcag attgtgcccc gccggatgct
     3901 ctcccgcatc tcggccgcga agagcgtgct catgaccccc gaggaggtgg cgaacgaggc
     3961 ggagacctac gacgacgaga ttgttgtccg cgtctacaag gaatatgaaa aactcctgcg
     4021 aggggccaac gccgtcgatt tcgacgacct tctcggcctg ccgattcgcc ttttcgacca
     4081 cgaccccggc gtcctcgaac gctaccagca acgattccgc tacatcatgg tcgacgagta
     4141 ccaggacact aaccgggtcc aatacgtcct tgtctccgcg ctggccgacc tccatcgcaa
     4201 tctttttgtg gtcggtgatc ctgaccagtc gatctatgca tggcggcagg cggatatccg
     4261 gaacattctc gatttcgaga aagattaccc ggctgcgacg cagatccacc tcgagattaa
     4321 ctaccgatcg acgcagcgga tcgtcgaggc tgcggatcgg gtgatccggg ataacgagca
     4381 acgcatcgac agaagaatcc gcaccattaa cgaggaaggc gagaaaatcc aggtccggga
     4441 gctctccgac cagcagcacg aggcccaatt catcgtgagt gagatacgac gcatgacggc
     4501 caggggagtc tgccgccccg aggacgtcgc tgtgatgtac cggaccacgg cgcagagccg
     4561 cgcgctggag gaagcgttcc gcaccagcga gttccgctac aggatcgtcg gcggagttcg
     4621 cttctacgag cgcaaggaag tgcgagacat ccttgcggtc ctgaga
//
LOCUS       DASYWM010000191         4033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_345602, whole genome shotgun sequence.
ACCESSION   DASYWM010000191 DASYWM010000000
VERSION     DASYWM010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345602"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..650)
                     /locus_tag="VGR29_07935"
     CDS             complement(69..650)
                     /locus_tag="VGR29_07935"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEV2073556.1"
                     /translation="MAQVQESESRTATPGDLSIVQSFLNSVDLEEGREDFGTPSGLVA
                     WIAGHGLAEPGYVPSEGDRLRAIEVREGIRDLLLPHGGDDVPKGTASRLNEAFGTVLL
                     TPVFAEDDGLRLQPAGTGLDAVFGRLLQIIYASSIEGTWQRLKVCRNDACRWAFYDAS
                     KNRSGVWCTMASCGSKMKARSYRRRVRSSSRSP"
     gene            764..1036
                     /locus_tag="VGR29_07940"
     CDS             764..1036
                     /locus_tag="VGR29_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073557.1"
                     /translation="MVNPSSFGRMQRTGIELAVGVLRHPGDAIGAAVDRVSALPYDSR
                     ESLRSEEQQLAGRGVSWTADAADDVELAQELAAARRRRAVRRSRDS"
     gene            1475..2203
                     /locus_tag="VGR29_07945"
     CDS             1475..2203
                     /locus_tag="VGR29_07945"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV2073558.1"
                     /translation="MSRHAASVSPQAHKPASCTLPAIRTGFRVTLPFAVGTMAIGATF
                     GALATSSGMSGITSLLMSIFVYSGTAQMLALDLMDSHAGVLVIWGGTLLVSLRYVLLG
                     FTMRGWFPRWPRWLVFSALYLSADQSWALTLGEREAGHRDTGFFLGSNLALFIGWTGG
                     TAIGALAGDHFGDPTAWGLHFAASVAFIGILAGVHRGRIDVLPWLISAAVAIGMERLV
                     GDHWHLIAGVVAGVAVGVSQGNVA"
     gene            2203..2454
                     /locus_tag="VGR29_07950"
     CDS             2203..2454
                     /locus_tag="VGR29_07950"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV2073559.1"
                     /translation="MDRQLLVTMLGMVTILYVTRISGYMLVGRMPASPALDRVLNQLP
                     GVTLVALVAPAVAQEALPGFSPLWSSGWLLFARTTSCCR"
     gene            2759..3967
                     /locus_tag="VGR29_07955"
     CDS             2759..3967
                     /locus_tag="VGR29_07955"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEV2073560.1"
                     /translation="MTGPKGIVAVLPFLVVLMLVSGSVVPQAASTAPANAFYNPPPDI
                     QDRHPGEIIRVEEMRSAFSSTRMWRMLYVSTNSADESVAVSALLVAPVAPPPDGGYPL
                     VAVGHGSVGIGQECAPSVDPYRPSGSGPSSYDLFVAPLVNAGYVVVMSDYQGLGAPGP
                     SSYLLGELEGRNILDSARAAFAFPGITLQPELLIWGQSQGGHAALFAGQLAPTYAPEL
                     SVLGVVAEAPAANLEAMFTHLIETNARGGIVSMPLMTVAAWAQEYPEIHIDALLTARG
                     KDILHNVVEDACLVPAFLVTLFDRPSSLIRPRALDRISTIVEANTPQTGPYAMPILIV
                     QGEADDAVPVETTRSFVDGMCRAGTNVMYRAYDGIGHIEVIDAAHADVLLWMEQVRNK
                     TIPIIPDYSC"
BASE COUNT          726 a         1219 c         1216 g          872 t
ORIGIN      
        1 gtcctgtgcc gcgctgatgc cacggcacag gacgcctcgc cacacccgag cagcgttcat
       61 tacggattct acggcgatcg agagctcgac cgcactcgcc tgcgatagga ccgcgctttc
      121 atcttgctcc cgcagcttgc catggtgcac cagacgcccg agcggttttt tgatgcatcg
      181 tagaaggccc atcggcacgc atcgttgcgg caaaccttca gccgctgcca ggtgccctcg
      241 atcgatgacg cgtagatgat ctgcagcagg cggccaaaca cggcatcgag acctgttccc
      301 gccggttgaa gcctcaaccc atcgtcctcg gcaaagaccg gggtgagcaa cacggtcccg
      361 aacgcctcat tcaagcgcga ggcggttccc ttgggcacgt cgtcaccgcc atgggggaga
      421 aggaggtcgc gtatgccctc acgcacctcg atcgctcgca accgatctcc ctctgacggc
      481 acgtaacccg gttccgccag gccgtgacct gcaatccacg ccacgagccc ggacggcgtg
      541 ccgaaatctt cccgtccctc ttccaggtca accgagttga ggaaagattg cacgatcgag
      601 agatctcccg gcgttgccgt gcgactttct gattcctgga cttgtgccat aaagtaacca
      661 ccaaagagac ttgacaggta acggcctgtc cgctactttg tgaataatac ctcgtcacca
      721 ccgtaatcgt ttatctggtg atatcaggaa aagaggcagc gccatggtca atccgtcttc
      781 gtttggcaga atgcagagaa ccggcatcga attggccgtt ggtgtcctgc ggcatccggg
      841 tgatgcaatc ggggctgcgg tcgaccgtgt aagcgccctt ccctacgaca gccgcgaaag
      901 cctgcgctcg gaggagcagc aactggcagg gcgtggtgtg agctggactg cggacgcagc
      961 ggatgatgtc gagcttgcac aggagttggc agccgcccgg cggcggcgtg ccgtccgtcg
     1021 ctcccgggac agctaggggc agaactcgct cccgggcctg ctgatgtaga tcttccgcct
     1081 gcgattggac cggatgctgg cattggagcc tgctgccgcc agtcgagcat gtcgtaaaag
     1141 ccgaccagga cgttgctcgt cttccgcacc aggaatatcg ttctcttgag gatcgcaagc
     1201 gtcgccgtac gaccttctgg tgcctgtgcc gagggcccaa agtaactgcc agaaagggtt
     1261 caccgggtat cgggcggacc gctacccggt gaacaaaaat tgtcaccact ctattgagtc
     1321 aggtggcaag tgcgctaaca aggggaccac tcgtggtcga ttagtcctca cccggtaaac
     1381 tgcaagcaat cctcacggca acagtaatcc ttacgcccga aatcatcctg tcaattgctg
     1441 tccctggcac gcaagtacct ggaatgtcag cgcgatgagc cggcacgcgg cttctgtttc
     1501 gcctcaagcg cataaacccg cctcatgtac gctcccagcg atccggacgg gattccgggt
     1561 cacgttgccc ttcgcggtgg gcacaatggc aattggcgct accttcgggg cgcttgctac
     1621 ctcgagcggc atgagtggca taacgtcgtt gctgatgtcg atcttcgtct actccggaac
     1681 ggcgcagatg cttgcactgg acctgatgga tagccatgcg ggcgtgcttg tgatttgggg
     1741 tggaacactg ctcgtgagcc tgcgctatgt gctgctcgga ttcaccatgc gcggctggtt
     1801 ccccagatgg ccacgctggc tggttttctc ggccctctac ctgagcgcgg accagagctg
     1861 ggcgttgacg ctcggtgaga gggaggcagg tcaccgggat acgggcttct ttctcggcag
     1921 caacctcgcc ctcttcatcg ggtggacggg tggcacggcg atcggtgcgc tggcggggga
     1981 tcactttgga gacccgaccg cgtggggcct gcacttcgcg gcctcggttg cattcatcgg
     2041 catcctcgct ggggtgcacc ggggccgcat cgatgtgctg ccatggctga tctcggcagc
     2101 cgtcgcgatc ggcatggagc gtctcgtcgg tgaccactgg catttgattg caggggttgt
     2161 cgcgggtgtc gccgtcgggg tgtcccaggg aaacgttgcg tgatggacag gcaacttctg
     2221 gtcaccatgt tgggcatggt gaccatcctc tatgtgaccc gtatcagtgg ctacatgctg
     2281 gtcggaagga tgccggcatc accggcgctg gatcgagtcc tcaaccaact gccgggcgtt
     2341 acgcttgtcg cgcttgtggc accggcggtg gcccaggagg cgttgccggg cttctcgccg
     2401 ctgtggtcgt ctggctggtt gttattcgca cgaacaacat cgtgctgtcg atgattactg
     2461 gcgtcggaat catcgcgctg ctcaggtgac cgtacatgag gcgggaagat gttgttgcca
     2521 gcaacgtcag gcagcccaag cattaccatc ggcctctcct gcgttctact ccatccggga
     2581 ttccagatgc cgcgtcgatg aacccgggct gcactgatgc ccaagtggcc accacacggc
     2641 acttgtatac tccgcatatc accgtcatgt cgatttatgt agcggatgcg gcatgtgatt
     2701 gaatcaatct ccttcatgcg tgctgcggat aatgctgcct gaaagaggct gccttacaat
     2761 gaccggtccc aagggcatcg tcgcggttct gccgttcctg gtcgtcctga tgcttgtgag
     2821 cggatctgtg gtccctcagg cagcgtctac ggccccagcc aatgcattct acaatcctcc
     2881 ccctgatatc caggatcgtc atcctggcga gatcattcgc gttgaagaga tgcgatcagc
     2941 gttttccagc acgcgaatgt ggcggatgct ctatgtctct acgaattccg ccgacgagag
     3001 tgttgctgtt tcggccctgc tggttgctcc cgtcgcacca cccccggacg gtggatatcc
     3061 cctggtcgcg gttggtcatg gctcagttgg gatcgggcag gaatgtgcgc cttcggttga
     3121 tccctaccga ccctccggga gtggtcccag cagctacgac ctgtttgtgg cgccactggt
     3181 caacgctggt tacgtcgtgg tgatgagcga ctatcagggc ctgggcgccc cgggcccttc
     3241 gtcgtatttg ctgggcgagc tggaaggacg gaacattctc gactctgcac gagcggcctt
     3301 tgcctttcca gggatcaccc tccagcccga gctcctgatc tggggacagt cccagggtgg
     3361 ccatgctgcc ctgtttgccg ggcaacttgc accgacctat gcgccggaac tctcggtgct
     3421 tggcgtcgtc gcggaggctc ccgccgccaa tctcgaggcg atgtttaccc atctcatcga
     3481 aacgaatgcc cggggaggta ttgtgtcgat gccgctgatg acggtcgcgg cgtgggcgca
     3541 ggagtatccg gagatccaca ttgacgcgct gctgactgcc cgtggcaagg acatactgca
     3601 caacgttgtc gaagacgcct gcctggtacc tgcgttcctg gtaacgctgt ttgatcggcc
     3661 gtcaagtttg atccgccccc gtgccctgga tcgaatcagc accattgtgg aagccaacac
     3721 gccccaaacc ggcccgtacg cgatgccgat cctcatcgtc cagggcgaag ctgatgatgc
     3781 cgtgcctgtg gagacgacaa ggagtttcgt cgatggcatg tgcagggccg gcaccaacgt
     3841 catgtatcgg gcttacgacg gcattggaca catcgaggtc atcgatgccg ctcatgccga
     3901 tgtgctcttg tggatggagc aggtgcgcaa caagactatt ccaatcattc ctgactattc
     3961 ctgttaattc catcccattc cggtttgctt cgagaagtcc ccataaatac gcgcgattcg
     4021 ccaggtgtcc atg
//
LOCUS       DASYWM010000192         7053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_232871, whole genome shotgun sequence.
ACCESSION   DASYWM010000192 DASYWM010000000
VERSION     DASYWM010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232871"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VGR29_07960"
     CDS             <1..726
                     /locus_tag="VGR29_07960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV2073561.1"
                     /translation="QRQVAMERDRRLFISAIAHDLRTPLFSLRGSLEGLEKGIATTPE
                     KRDRYIAVAQDKAAVLERLISDLFTFTRMEYMEEAPNRTLLDLDVFLPHLIEGMRPRA
                     ETNSIHIALNAASTPVKVEADSHLLTRAVENLLDNALRHTDVGGTVRVSWWESKSSAS
                     ISIQDTGPGIADVDLPHLFDPLYRGESSRNRKTGGAGLGLTIARRIVEAHGGTLVAGN
                     NMDGGAIFTMTLPKERSADHPAV"
     gene            complement(961..2166)
                     /locus_tag="VGR29_07965"
     CDS             complement(961..2166)
                     /locus_tag="VGR29_07965"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEV2073562.1"
                     /translation="MANETPTESTLLVGQSGGATAVINASLAGVVDAAWRSGEFERVL
                     GLRNGIEGLFQERFVDLTRQDQDLINRIGRTPSAALGTGRYKLRDEDLDRAVIAMRRL
                     GVTAFVYIGGNDSADTAHRLEAHAHTIGYELRVMSVPKTIDNDLPETDHCPGYPSIAR
                     YLGNAVRDATYDTLASPQLYPVKFVEVMGRDAGWVAAAGALGFSGAEIDLAPLVLLPE
                     RAPQSVDDVLAAIDADVRRRGFSIVVVPETMHTSSGRHFGGDEPEYVDGFGHPYFPST
                     GAAMTRLVSQKLQLRARYDKPGTAARMSIALASEVDLDEAYRLGAGAVDRMVDGASGF
                     MTAISRTSDDPYTYDIIAVPLEKIANQVRHLDDAFIGADGMSVTPAFHAYMRPLLGKE
                     PFPPYGRFG"
     gene            complement(2389..3039)
                     /locus_tag="VGR29_07970"
     CDS             complement(2389..3039)
                     /locus_tag="VGR29_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV2073563.1"
                     /translation="MARSPSPSPFRSLSASTSGSDVLVELLSSRVRAAVLGFLVAHGE
                     DRFSLTELARFLDLSISSVQHECYKLERLKVLKGRREGASRRYWLDHETPFVAQLIDL
                     VATVLGPECLLGHALADVQGLQGALLAMTPQPQLVLIGELGLEELEIVQGRAASLLGV
                     DQETIDIAFYDSASWVDHRAGDSALMSRLRQTEPIMLVGDGAKIWPGWPRMNTSSF"
     gene            3246..4658
                     /locus_tag="VGR29_07975"
     CDS             3246..4658
                     /locus_tag="VGR29_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV2073564.1"
                     /translation="MRRPLQAWEATISTTIGLVAVVLLVLANGFFVATEFALVSVRRT
                     RIQQLVSEGDRRATGVLDRLNHLDTYIAATQLGITISSLALGWIGEPAVATLMHPVID
                     QLPFEIGSRTTHAISFILAFSLVTALHIVIGELAPKSLALQRPDQTSLVVAGPIHTFF
                     VIFRPFIFSLNWIGNQVVKLIGIQPASGHELVQSAEELMLSIDASREAGLVNQTAHDI
                     VGRAFVFTDMQARHVMVPRTEVTALPLEATLEDVINIMAESAFTRLPVYEGDTDNIVG
                     IIKTKRLLPLFLQKLRDRQDALHSGETADPEIPFDVREYMFEPTVVPETLAAAEVLTL
                     MREKRVQIAVVIDEYGGTAGIVTLKDIVTQLVGRVQDEEDTSGEFGPDTNGVLYLDGL
                     MNLTELREEHMIDLQNEDYDVETLGGYVFSSLGRPGRVGDEVTSVGGVTFRVEEMDGL
                     RISRVSVQPAAAHRAVHAPA"
     gene            4737..5309
                     /locus_tag="VGR29_07980"
     CDS             4737..5309
                     /locus_tag="VGR29_07980"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2073565.1"
                     /translation="MNSAVSARKRDRLTPAEFTLLGMISLAPEDGHIHGYDLNRRMTE
                     GAIGAIIRLESGMLYHYLKKLTQRGLITSTTEHQEGRPTRHLHALTETGRATLGAWLD
                     TPVQATREMRLEFLLKLWFARHTGREEANILIRSQRAVIQSLIDSLEQQLRETPDATA
                     DDRFMRTVIGLRLAQNRAVASWLDELEAAP"
     gene            5309..6445
                     /locus_tag="VGR29_07985"
     CDS             5309..6445
                     /locus_tag="VGR29_07985"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HEV2073566.1"
                     /translation="MVPPPSDMVWNTFPRVIPEARKGAPPLPFHRLLPGYEPTPIVEL
                     TDLAREVGIGRITAKIETTRLGLPAFKILGASWAIYRELCRRLPGLEERWESLDDLRQ
                     QITPLGELTFVAATDGNHGRAVAHMARQLGQRAHILVPAGMAPARIDAIESEGAAVEV
                     IDGTYDDAIVASAALMDRSHLVISDTAWDGYQVVPRDVIAGYSTIFAEAAEQMDDAPT
                     QLFIQMGVGALGTAAILASRPWKAKVVSVEPKDAACILASIREKELTEVPGPHRSIMA
                     GLNCGIPSPIAWPLLQTGIDLALAIPDDAARSAMRMLADAGVVAGETGAAGVAGLMSL
                     ATSPNWPEIRKRLKMDASSHVLVIITEGATDPAAWEAITGRRLL"
     gene            6521..>7053
                     /locus_tag="VGR29_07990"
     CDS             6521..>7053
                     /locus_tag="VGR29_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV2073567.1"
                     /translation="MPGRHHSSRHVPMLLLLAMLPTLLALPFLPQDVSAEVVCDDEVV
                     SRATPAVTAAPVPDAAFPEDGGTLTILAAASLIDAFAEMEQRLEEAHPGLDIVIETAG
                     SQTLVTQLEQGARADVLATADMRSMEGASASGLITGGPVAFTTNRLVIVAPADNPAGV
                     DSLDHLAGEDLSLILASG"
BASE COUNT         1348 a         2251 c         2148 g         1306 t
ORIGIN      
        1 cagcgccagg ttgccatgga gcgcgaccgt cgcctcttca tcagcgcaat cgcgcatgat
       61 ctccgcacgc cgctcttctc gctccggggt tcactggaag ggctggagaa gggaatcgcc
      121 acgacacccg agaagcggga ccgctatatc gctgtcgccc aagataaggc tgccgtgctc
      181 gagcggctca tatctgacct tttcacgttc acgcgcatgg aatatatgga agaggcaccc
      241 aaccgcacgt tgctcgacct cgacgtattc ctgccccacc tgatcgaagg gatgcgacca
      301 cgggccgaaa cgaacagcat tcacatcgcg ctgaatgcag catccacacc ggtcaaggtg
      361 gaggccgaca gccacctgct cacgcgggct gtcgagaacc tgctcgacaa cgccctgcga
      421 cacacggatg tgggtggcac cgtacgcgtt agctggtggg aatcgaagtc gagtgcgagc
      481 atctcaattc aggataccgg tccagggatt gcggacgtgg acttgcctca tctcttcgac
      541 ccactttacc gtggtgaatc atcgcggaac cggaagaccg gcggtgccgg actcgggctg
      601 acgatcgccc ggcgtatcgt ggaggctcac ggcgggacac tcgtagctgg caacaatatg
      661 gatggagggg cgatcttcac aatgacattg ccaaaggagc gtagtgcgga tcatccggcg
      721 gtatagcagg gtaatctgca tatcgtggca tctccattcg gaggaccctt tgtcattccg
      781 atcgtcgcct tgcactgtag tttcgcgacc cagctcctcc gatgaccggc ttcgccgggg
      841 gattcttcgc ctgtgggccc agaatgacga tggagagcat ggttcctcga cacggccgag
      901 atgacaattt cttcgcgccg aatgcaggta acagcgaaac cgcttacctg cccagacact
      961 ttagccaaac cggccgtatg gcgggaaagg ctccttgccc agcagtggcc gcatgtatgc
     1021 gtggaacgcg ggtgtgaccg acatcccgtc cgcgccgata aaggcgtcat ccaggtggcg
     1081 cacctggttg gcgatcttct ccagcgggac ggcgatgatg tcgtacgtgt acggatcgtc
     1141 ggaggtgcgg gagatcgcgg tcatgaatcc cgacgcgccg tccaccattc gatccacggc
     1201 cccggcgcca agccgatacg cctcgtcgag atcgacctcc gaggcgagcg cgatcgacat
     1261 ccgcgctgcg gtgccgggct tgtcgtagcg ggcacggagc tggagctttt gggaaaccag
     1321 gcgtgtcatt gccgcgccgg tactcgggaa atagggatgc ccaaagccgt cgacgtactc
     1381 cggctcgtca cccccgaagt gccttccaga tgacgtgtgc atggtttccg gcacgacgac
     1441 gatcgagaaa ccgcgccgcc ggacgtcagc atcgatcgcc gcaaggacat cgtccaccga
     1501 ctgcggggcc cgctcgggaa gcagcaccag cggggcgaga tcgatctccg ccccggagaa
     1561 ccccagggca ccggcggcgg cgacccagcc ggcatcccgc cccatgacct ccacgaactt
     1621 gacgggatag agctgtggcg acgccagcgt gtcgtaggtc gcatccctca ccgcattgcc
     1681 aagatagcgg gcaatggaag ggtaaccggg acagtgatcg gtctccggca gatcgttgtc
     1741 gatcgtcttc ggcactgaca tcacgcgcag ctcgtagccg atagtgtggg cgtgggcttc
     1801 gaggcggtgg gcggtatcgg cggagtcgtt cccgccgatg tagacgaagg cagtgacgcc
     1861 gagccgccgc atcgcgatca cggcgcggtc gagatcctcg tcacgtagct tgtagcgtcc
     1921 agttcccagg gcagccgacg gtgtccgccc aatgcggttg atgagatcct ggtcctgccg
     1981 ggtgagatcg acgaatcgct cctgaaagag cccttcgatc ccgttgcgca ggcccagcac
     2041 ccgttcgaac tcaccactcc gccaggcggc atccacgacg ccggcaagac tggcgttgat
     2101 gaccgctgtc gcgccgccgg actgaccgac aagcagtgtg gattccgttg gggtctcatt
     2161 cgccatcgtg ctcacagaaa tactcttccc ttactcaact atgtcaccca ttcttgactg
     2221 cgggagcaca caacggaccg cgtccgccca attcctacgg aattgctaat ttgcccggag
     2281 ggagatgacc ttggcagagc caatgctgac agggtcattt ttccatgtgt cgccatcgtg
     2341 atagtcgcgt acaactaatg cgcttcgcgt aggattcttc gcatccgctc agaatgacga
     2401 ggtgttcatc cggggccaac ccggccagat cttggcgccg tcgccgacaa gcattatcgg
     2461 ctccgtttgg cgcagcctcg acatcagggc agaatctccc gcacgatggt cgacccagga
     2521 ggccgagtcg taaaaagcga tatcgatcgt ctcctgatcg acacccagga gggaggcggc
     2581 tcggccctgg acgatctcca gttcttccag cccaagctcc ccgatgagca ccagctgcgg
     2641 ctgcggcgtc attgccagca gcgctccctg gagcccctgg acatcggcca gcgcgtggcc
     2701 tagcaggcac tcgggcccga gcacggtcgc gacaagatcg atcagctgcg caacaaatgg
     2761 cgtctcatga tccagccagt agcggcggga ggcgccttca cgccggccct tgaggacctt
     2821 cagccgctcc agcttgtagc actcgtgctg cacgctgctg atcgaaagat cgagaaagcg
     2881 ggccagctcg gtgagcgaaa aacgatcctc gccatgtgca acaaggaaac cgagcaccgc
     2941 cgcccgaacc cgggatgaca gcaattcgac gagcacatcc gaaccagatg tggatgcgga
     3001 tagggatcgg aagggagacg gcgacgggga gcgtgccatg atacgcgggc tgccctgctt
     3061 tcagcgcgag aacaaacact ttcggtcatc gtagcacgtc tccacccgga cgcgaacgta
     3121 cacccttgaa cctggtggag tgcgggcgcg ctttcctcta ctattgaacg tgctcgtcag
     3181 cgacgcgccc gccccggcct tcatgccggt tctttcccat actccacatg ggtccgcggg
     3241 ccctcgtgcg tcgtccgctc caggcttggg aggccaccat tagtaccacc attggactcg
     3301 ttgccgtcgt tctcctcgtt ctggcgaacg gctttttcgt cgcaaccgag ttcgcacttg
     3361 tttccgttcg tcgtacccgt atccagcaac ttgtctccga gggagaccga cgtgcgaccg
     3421 gtgtgctgga ccggctcaac cacctcgaca cctacattgc cgcgacccag ctcggcatta
     3481 ctatctccag tctcgcgctc ggctggatcg gcgagcctgc cgtcgccacg ctcatgcacc
     3541 cggtcatcga tcaactgccc ttcgagatcg gcagtcgcac cactcatgca atctccttca
     3601 tcttggcatt ctcgctggtg accgcactcc atatcgtgat cggtgagctt gcgccgaaga
     3661 gcctggcgct gcagcgcccg gaccagacct cgctggtggt ggctggccca atccacacct
     3721 tctttgttat cttccgcccg ttcatcttca gcctgaactg gatcggaaac caggtggtta
     3781 agctcatcgg catccagccg gcctccgggc acgagctggt gcaatcggcc gaggagctga
     3841 tgctttcgat cgacgcgagc cgcgaggctg gcctcgtcaa ccagaccgcg cacgacattg
     3901 tcggtcgtgc cttcgtcttc acggacatgc aggcacgtca cgtgatggtg ccgcgaacag
     3961 aggtcacggc cctcccactt gaggcaacgc tggaggatgt gatcaacatc atggcggagt
     4021 ccgccttcac ccgcctcccg gtatacgagg gagacacgga taatatcgtc ggcattatca
     4081 agaccaaacg cctgctcccg ctcttcctgc agaagctccg ggatcgccag gacgcacttc
     4141 atagcggtga gacagccgat ccggagattc ccttcgacgt ccgggagtac atgttcgagc
     4201 caaccgtcgt accggaaacc ctggcagcgg cggaggtgct gacgctgatg cgcgagaagc
     4261 gcgtccagat cgcggtcgtc atcgacgagt acggaggtac cgccggcatc gttacgctga
     4321 aggacatcgt cacccagctt gtgggacggg tgcaggacga agaggacaca tcgggagagt
     4381 tcggcccgga caccaatggc gtgctctatc tcgatgggct gatgaacctg accgagctcc
     4441 gcgaggagca catgattgac ctccagaacg aggactatga tgtcgagacg ctgggtggat
     4501 acgtcttctc ctcgctgggt cgccctggca gggtcggtga cgaggtcacc tctgtcggtg
     4561 gcgttacctt tcgggtcgag gagatggacg ggctccgcat ctctcgtgtc agcgtccagc
     4621 ccgcagcagc acaccgggcg gtgcatgctc ccgcataact tcacattccg ggagactgat
     4681 cgaagacaga ggcgccgcac cggtgcgtag cgacgctcac gaaggaccag atgatcatga
     4741 actccgccgt cagcgcgcgc aaacgcgatc gcctcacccc ggcggagttc accctgcttg
     4801 gaatgatctc gctggcgccg gaggatggac acattcacgg gtacgacctg aaccgccgga
     4861 tgacagaggg ggcgatcggt gcaatcattc ggcttgaatc cgggatgctc tatcactacc
     4921 tgaagaagct gactcaacgc gggctaatca cctccaccac cgagcaccag gagggccgcc
     4981 cgacccgtca cctccatgcc ctgacagaaa caggccgcgc aacgctcggt gcatggcttg
     5041 acacacctgt ccaggcgaca agagagatgc ggttggagtt cctgctcaag ctgtggtttg
     5101 cccggcatac cgggcgtgag gaggccaata tcctgatccg tagccagcgt gctgttatcc
     5161 aatccctcat cgattcgctg gagcagcaac tccgggagac acccgatgcg acagccgatg
     5221 accgtttcat gcgcacggtg atcgggcttc gcctcgccca gaaccgcgcg gtggcttcct
     5281 ggctggatga actggaggca gcaccgtgat ggttccgcca cccagcgaca tggtctggaa
     5341 tacctttccc cgggtgattc cggaagcccg gaagggcgct cccccgctcc ctttccatcg
     5401 gctcctgccg gggtacgagc caacacccat cgtcgagctg accgatctcg cccgggaggt
     5461 gggtattggc cggattaccg ccaagatcga aacgacccgg ctcggcttgc ccgcgttcaa
     5521 gatcctcggg gccagctggg ccatctatcg agagctctgc cgtcgccttc ccggtcttga
     5581 ggagcgatgg gaatcccttg acgatctccg ccaacagata acgccgcttg gtgagctgac
     5641 cttcgtcgcg gcgacggacg gcaaccatgg gcgcgcggtt gcccacatgg ctcgtcaact
     5701 tggtcaacgg gcccacatcc tggtcccggc cgggatggcc ccggcgcgca tcgatgcgat
     5761 cgaaagcgaa ggggctgcgg tcgaggtcat cgatggcacc tacgacgatg cgatcgtggc
     5821 atcagcagcg ctcatggaca gaagccatct ggttatctct gatactgcct gggacggcta
     5881 tcaggtggtc ccgcgcgatg tgatcgccgg gtacagcacg atcttcgcgg aggccgctga
     5941 gcagatggac gatgcgccga cgcagctctt catccagatg ggggttggag cgctcgggac
     6001 cgcggccatc cttgcctcca gaccgtggaa agcaaaggta gtcagcgtgg agccgaaaga
     6061 cgcggcgtgc atcctggcgt ccatccgcga gaaggagctg acagaggtgc ccggccctca
     6121 ccggtcgatc atggcggggc tgaactgcgg catcccctct ccgattgcct ggccgctgct
     6181 ccaaaccggt attgaccttg ccctggcgat cccggatgac gccgcacgga gtgcgatgag
     6241 gatgcttgcc gacgcaggcg ttgtcgccgg tgagacgggt gcggcgggtg tggcggggct
     6301 gatgtcgctg gccacatccc ccaactggcc ggagatccgg aagcggctca agatggacgc
     6361 atccagtcac gtccttgtga tcatcaccga aggcgccacc gaccccgccg catgggaagc
     6421 gatcaccggc cgaagactcc tgtaatgccc cgttctacgt ttgaagaaac ctctgtatac
     6481 tgaggtccta ttcaatgatt gactaatcat ggagcggctc atgcccggaa gacatcactc
     6541 gtctcgtcac gttcccatgt tgttgctcct ggccatgttg cccaccctgc ttgcgctccc
     6601 gtttctccca caggatgtct cggccgaggt ggtttgtgac gacgaagtgg tttcccgagc
     6661 aactcccgcg gtgaccgctg cgccagtgcc cgatgctgcg ttcccggaag atggcggaac
     6721 cctgacgatc ctggcggctg catcactcat cgatgcattc gccgagatgg aacagcggct
     6781 ggaggaggcg catccgggtc tggatatcgt gatcgagacg gcaggctcgc agacgctcgt
     6841 tacccagctg gagcagggag cccgggcgga cgtgctcgcc accgccgaca tgcgatcgat
     6901 ggagggagcg tccgcatccg gtctcatcac aggtggcccc gttgccttca ccacaaaccg
     6961 gctggtcatc gtcgccccgg cggacaaccc ggccggggtc gactcactcg atcatctggc
     7021 tggcgaggat ctctcgctca tcctcgccag cgg
//
LOCUS       DASYWM010000193         4189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_533394, whole genome shotgun sequence.
ACCESSION   DASYWM010000193 DASYWM010000000
VERSION     DASYWM010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533394"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VGR29_07995"
     CDS             complement(<1..525)
                     /locus_tag="VGR29_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2073568.1"
                     /translation="MPRPLKIGVQLPEVEYEYTWPQLKEMAVIAEEIGLDSIWLGDHL
                     MYRYADPKRAPRGPFEAWSTLAALAAVTERVQLGPLVASALFHSPPMITKKAATIDQI
                     SNGRFVLGLGAGWHEPEYRGFGFPYDHRFSRFAEAFGIIRELMTTGESTFHGEFYDID
                     GALLFPKPVQPGGPP"
     gene            766..1791
                     /locus_tag="VGR29_08000"
     CDS             766..1791
                     /locus_tag="VGR29_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073569.1"
                     /translation="MFAISAARRNSRPQREIILTAGMLVVTKIATMFLAKDLLFALLH
                     AGEDGPEVARIGLFTAVIFILIYGNVVSLMTRIGYNLRRMRHSPPAFGDIVARHWDRA
                     EPIVALVPSYKEDARTIRQTLLSAALQHHPRKRVVLLLDDPPHPKDRESAELLVAARR
                     VLGEITTLLQEPKDLVGKAFRGFDARQGSGPVDAQTELRELLGVYNGLVAWMDRCVAE
                     GYPSDHTDHHFLAITFQEHKSLLIDSARRLVVDLHGRSTTEDVAREYQRLTDLFDVEI
                     TRLSSASATGTCRRRRTRRRTSTRTLGCSGRRCVSAGTLMESVWMSPTGLQADAASSR
                     SRMPRLS"
     gene            1809..2642
                     /locus_tag="VGR29_08005"
     CDS             1809..2642
                     /locus_tag="VGR29_08005"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2073570.1"
                     /translation="MLRPDYALRLSSVFAEPGNELVAVVQTPYTAIPNAPGVLERIAG
                     ATTDIQYIVHQGFTWCDATFWVGANALLRKRSLDDIRTEEEEGGYLVSKFIQDRTVIE
                     DTESTVDLAARGWRLHNYPERLAFSATPPDFGSLLIQRRRWANGGLLIIPKLVQNALT
                     RPFQAITIPSFLVRLHYLSSIATGSIGMLTLLFLPVDAELLSAWLPVLAAAYFSMYWR
                     DLLLNGYLPGDILRVSAFNIMLLPINLAGVLKSVQQGVTGTRTPFARTPKVEGRTAAP
                     A"
     gene            2718..2894
                     /locus_tag="VGR29_08010"
     CDS             2718..2894
                     /locus_tag="VGR29_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073571.1"
                     /translation="MYAAFSLVIAAGVGYAVLVFIGPKASWEDGTRSMRMGVGGLRQR
                     IENWASFGENVRSR"
     gene            complement(3064..>4189)
                     /locus_tag="VGR29_08015"
     CDS             complement(3064..>4189)
                     /locus_tag="VGR29_08015"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2073572.1"
                     /translation="LFYGHYDVQPADPLELWKSDPFEPEIRDGRIYARGVADNKAQHF
                     SHIAAIRAWMKATGSLPVNVRVLLEGEEEVGSPHLDETVRAHRDLLMADLIYTSDGPI
                     TDERYPEISFGVRGLLYVELRATGPNRDLHSGHWGGVAPNPIWQLVRLLNTMIDDQNR
                     ILIEGFYDNVLEPTPGARAAMEKLPFDTQETIGAIGITEMVPPVDLPYADRIMAQPTL
                     NIAGFTGGYGGPGSKTIIPSKATVKIDMRLVPDQTPAEIWEKIGAHVEKHGDGVEIVQ
                     LDGGMLPSFTPVEHPLADVVRDAVEAGFGRRPVDIPSVGGSLPDATWTKTLGLPSFLV
                     PYGAPEQANHSPNESYRVERLWQGITTSATLLSLLAERES"
BASE COUNT          746 a         1242 c         1362 g          839 t
ORIGIN      
        1 cggcgggccg ccgggttgga ccggcttcgg gaagagcaag gcgccgtcga tatcgtagaa
       61 ctcgccatgg aaggtggatt caccggttgt catcagttcg cggatgatgc cgaacgcttc
      121 ggcgaagcgg ctgaagcggt ggtcgtaggg gaagccgaag ccgcgatatt ccggctcgtg
      181 ccaccctgcg ccaagcccga gcacaaagcg gccgttgctg atctggtcga ttgtggcggc
      241 cttcttggtg atcatcggcg ggctgtggaa gagggcactg gcaacaagcg gaccgagttg
      301 aacgcgttct gtgactgcgg cgagggcggc aagcgtgctc cacgcctcga aggggccgcg
      361 cggggcacgt ttgggatccg cgtagcgata catcaggtgg tcaccgagcc agatcgagtc
      421 cagcccgatc tcctcggcaa tgacggccat ctctttcaac tgcggccagg tgtattcgta
      481 ctcaacttcg gggagctgaa ccccgatctt tagtgggcga ggcatgggag cacctccgtg
      541 caagagcgag cgacatctct ggcgccatgc tacccgatac aggtactcgg ggaatcgctc
      601 cctccctctg ggccagggtg gggaatgccg gcagcagtat acgaaagtgc ctttgacgat
      661 atacggatga tccactagca ttggcaactt cgctcgggcc tggctcgggc gacgttgttc
      721 ttgcgccaca gtcagatgtg ctgctcgtat gctggggacc cgtatatgtt cgcgatatct
      781 gctgcccgac ggaattcccg tccgcagcgg gagatcattc tcaccgccgg tatgctcgtc
      841 gtcacgaaga tcgccacgat gttcctagcg aaagatttac tcttcgccct cctccacgct
      901 ggtgaggatg gcccggaagt ggcaaggatc ggcctcttca ctgcggttat cttcatcctc
      961 atctacggca atgttgtctc tcttatgacg cgtattgggt acaacctgcg ccggatgcgg
     1021 catagccctc ctgccttcgg ggacatcgtt gccaggcact gggacagggc cgagccaatc
     1081 gtcgcgctgg tgccttccta taaggaggat gctcgcacga tccggcagac cttgctctcg
     1141 gctgcgctgc agcaccatcc gcgcaagcgt gtcgtgctcc tgctggatga cccgccgcat
     1201 cccaaggacc gcgagagcgc tgagctgctg gtggccgctc ggcgggtact cggcgagatc
     1261 accaccttgc ttcaggagcc aaaggacctg gttgggaagg catttcgagg gttcgatgcg
     1321 cgacaaggta gcggccccgt cgatgcccag actgagttaa gggagcttct cggtgtgtat
     1381 aacgggctgg ttgcatggat ggatcggtgc gtggccgagg gctatccgag cgatcacacg
     1441 gatcaccact tccttgccat cacgttccag gagcacaaat cactcctgat cgacagtgca
     1501 cgtcggctgg tggtggatct gcacgggaga tcgacgacgg aagatgttgc ccgcgagtat
     1561 cagcggctga cggatctctt tgatgtcgag atcacacgtc tttcgagcgc aagcgctacc
     1621 ggaacgtgtc gcaggaggcg aacaaggcgg cgaacctcaa cacgtacatt gggttgctcg
     1681 ggcagacggt gcgtgagcgc tggtaccctg atggaatccg tctggatgtc gccgacggga
     1741 ctccaggcgg acgcggcgtc ctcacgatcc cggatgccgc gtctgtcctg acgcttgatg
     1801 cggacagcat gctgaggcca gactacgcgc tccggctctc ttccgtcttt gccgaaccgg
     1861 gcaatgagct ggtcgcggtc gtgcaaacgc cctacaccgc catcccaaac gcgcccggcg
     1921 tcctggagcg gatcgcaggt gcgacgacgg acatccagta catcgtgcat cagggattca
     1981 catggtgcga cgcaactttc tgggttgggg cgaacgcgtt gctgcggaag cgatccttgg
     2041 atgacatccg gaccgaggag gaggaaggcg gatacctcgt ttccaagttc atccaggacc
     2101 gcacagtgat cgaagacaca gagtcgacgg ttgatctcgc agcacgtggt tggagactgc
     2161 ataactatcc cgaaaggctc gccttcagtg caactccacc agatttcgga tccttgctga
     2221 tccagcgacg gcgatgggcc aatggcggat tgttgatcat ccccaagttg gtccagaacg
     2281 cattgacgcg gcccttccag gcgatcacga tcccgtcgtt cctggtgcgg ctgcactacc
     2341 tctcatcgat cgccacaggc agcatcggta tgctgacgct gctcttcctg ccggttgatg
     2401 ccgagctcct ctcggcctgg ctgccggtgc tggccgccgc ctacttctcg atgtactggc
     2461 gagacctctt gctcaacggg tatttgccgg gcgatatcct gcgggtgtcg gccttcaata
     2521 tcatgctgtt gccgatcaat ctggccggtg tgctgaagtc ggttcagcag ggcgtgacag
     2581 ggacgcgaac gccattcgcc cggacaccga aggtggaggg acgtaccgcg gcgcccgcct
     2641 agtcggtcat ttcgctctgg ctgctgtggg gaggggcgtt cgtcgcggga gttctgcacc
     2701 tcgcgcggga gcgatggatg tatgcggcct tctcgctggt tattgccgcc ggggttggct
     2761 acgccgtgct ggtctttata gggccgaagg caagctggga ggatggtacg cgctcgatgc
     2821 ggatgggcgt cggcggtctt cggcagcgca tagaaaactg ggcgtccttc ggtgagaacg
     2881 tgcgttcccg ttagaaaccc actgccaaat ccacagctgc gcctacaccg gacttcactg
     2941 atcctctcgt tccgctcaac gatccatacg agtaggcgtc atgccagatc ggtcatccca
     3001 tccgctgaga gtattgtcgg cgttccatat ctcattggat cgcctccctc cgatctttgc
     3061 cactcaggac tcccgctccg ccagtaacga caacagcgtt gcggaggtgg tgatgccctg
     3121 ccagaggcgc tcgacgcggt agctctcgtt gggggagtgg ttggcctgct cgggtgcgcc
     3181 gtaggggaca aggaacgacg gtagcccgag tgtcttcgtc caggtcgcgt cgggcaggct
     3241 accgccgacc gatggaatgt cgaccgggcg gcggccgaaa cctgcctcga cggcatcgcg
     3301 cacgacatcg gcgagcggat gctcgacggg ggtgaacgag ggcagcatgc caccgtccaa
     3361 ctggacgatc tcgacaccgt caccgtgctt ctcgacgtgc gcgccgatct tctcccagat
     3421 ctcggccggg gtctggtcgg gcaccaggcg catgtcgatc ttcaccgtcg ccttcgacgg
     3481 aatgattgtc ttgctgccag gaccgccata gccaccggtg aacccggcga tgttcagcgt
     3541 cggctgggcc atgatccggt cggcgtaggg aagatcgacc ggggggacca tctcggtgat
     3601 gccgatggcg ccgatggtct cctgcgtatc gaagggcagc ttctccatcg ccgcccgggc
     3661 gccgggtgtg ggctccagca cgttgtcgta gaagccctcg atgaggatgc gattctggtc
     3721 gtcgatcatc gtgttcagga gccggacaag ctgccagatc gggttaggcg cgacacctcc
     3781 ccagtggccc gagtgaagat cgcgattggg accggtggcg cggagctcca catagagcag
     3841 gccacgcacg ccgaaggaaa tctccgggta gcgttcgtct gtgatcggac catctgaggt
     3901 atagatgaga tcggccatca agaggtcgcg gtgggcgcgg acggtctcgt cgagatgcgg
     3961 gctgccgacc tcctcctctc cctccagcag gaccctgaca ttgacgggga gtgagccggt
     4021 ggccttcatc caggcccgga tggcggcaat gtggctgaag tgctgcgcct tgttgtcggc
     4081 gacaccgcgg gcgtagatgc ggccgtcgcg gatctccggc tcaaagggat cggatttcca
     4141 gagctcgagc ggatctgccg gctggacatc atagtgcccg tagaagagg
//
LOCUS       DASYWM010000194         5639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_364545, whole genome shotgun sequence.
ACCESSION   DASYWM010000194 DASYWM010000000
VERSION     DASYWM010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364545"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..1602
                     /gene="nadA"
                     /locus_tag="VGR29_08020"
     CDS             319..1602
                     /gene="nadA"
                     /locus_tag="VGR29_08020"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEV2073573.1"
                     /translation="MVQQTAQSKTMWPASLDTNKGGGTAVAERPTTATMWFSEPQLEV
                     ASCTPEEGVGAYDRLKAYGVGSLRQERIPREYFKMQAEERDARIFALKEELGARLTIL
                     GHHYQRDEVIQFADFQGDSYKLSQEAAGVTDAGFTLFCGVHFMAESADILGGPNQHVI
                     LPNLEAGCSMADMAKPEDVEAAWSVLAGLGIDSVIPVTYMNSAASIKAFCGRNGGIVC
                     TSSNAMRVYDWAFERGERIMFFPDEHLGRNTAVKKGIPQDQMVVWDPFQPMGGNTEED
                     LLNARVILWKGYCSVHARFSVEQIEKARVDYPDVNVIVHPECRVEVVQAADCDGSTEY
                     IIDTIRNAPAGTTWAVGTEINLVRRLQSQMPDKKIFCLDPVICPCSTMYRIHPAYMLW
                     ALEHLVRGEIVNEIKVDPQTSADAKVALDRMLSVP"
     gene            1620..2078
                     /locus_tag="VGR29_08025"
     CDS             1620..2078
                     /locus_tag="VGR29_08025"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV2073574.1"
                     /translation="MPIEVGVDAPDFMLKTSTGEDFRLSDLQGMLRVMLIFYPKDFTP
                     VCTDQLIQIRKNLKHLRKAGIEPVGVNPDDAESHERFRKAHGLNFELLVDEEAEVARA
                     YGALKPDGEGILRSVIVVGRNGKIAYSKEGKPPWQLILNALRQVSDEPHP"
     gene            complement(2215..3300)
                     /locus_tag="VGR29_08030"
     CDS             complement(2215..3300)
                     /locus_tag="VGR29_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEV2073575.1"
                     /translation="MMLDSLIDQFEDEELHRSYTVMALFDDIIDAESSLMCLRRLKQP
                     ADQISIIFRERVLDPDSRTPYRAVLSEVVAKNALDVVGSWLQGLASLVLPDRATYLAA
                     GPIGAILATIRESRQHGEDDDSTVSPLRSLPTRQLTRTFQAFGFERNESTYVEQRVVA
                     GSPLIAVTSETIDILRTAHQIFTENSPVYIGFTQTESTISSQAAKLLATGPRGTGAVV
                     IADAVSPLRHLIWDPRLQRGPRDLRHRLVRSQYGEIIGRVEDLLFEPEPVNPPPELLY
                     RGISDDQLLLRYIVVRVGRGLGRQRLAIPSERISITPDGVTVAVTHEELASAPRYDSE
                     AQLSRQEEATIRRHYNEPFYWISGVDV"
     gene            complement(3297..4478)
                     /locus_tag="VGR29_08035"
     CDS             complement(3297..4478)
                     /locus_tag="VGR29_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2073576.1"
                     /translation="MTAQQPYLIRVTISTIAIIAIVASAWMIWQIRSILLLLAIGILF
                     AAVIEPLVFRLRRAGLRRAQAILVVYLVLFTILGVALYYLVPLLVKQLASFDAAVPEI
                     FDNLRQQALQNESSLVRTSGFRVLMRIEQGYNNIRNSPEVGRDQAFSVVNTVFGFTLT
                     TISMLIVTFYWMVEKVTIKRVVLGLFPFSRRTRVHAIWDEIEYRIGGWTRGQLLLMLT
                     VGMISGIIYYGIDLRFWLALAIFAGLTEAIPFIGPIIGGSAAALVALADSPQKALIVI
                     GAAFLMQQLEGAFLVPRVMKNAVGMTPLTVILAILVGTTLGGPAGALIAIPIGAAVQV
                     LVGSLLRSRDDFIASELRTLDVKPLSPGHFDTPFVPPGQGRFIVHSAARRAPAEGEVR
                     A"
     gene            complement(4571..>5639)
                     /locus_tag="VGR29_08040"
     CDS             complement(4571..>5639)
                     /locus_tag="VGR29_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073577.1"
                     /translation="RFFVLDNPDPEKVRATLETVDLSRTLVNVVSKSGQTAETAANFL
                     VVRDALIQAVGREQAARQIVATTDPETGILRQMADQEGYRTFPVPPGVDGRQTVLSAV
                     GLIPAALAGVDIHQLLAGAAAMRARASSSDIRDNPAYLLAALSVLADTNHDKSMLVTM
                     PYADGLFGLSDWFRQLWAESLGKKLSTDNEVVFAGQTPIKALGAIDQHSQIQLYTEGP
                     NDKLIQLIAVGAYRETVEIPSPPESATELSYFQGGELGQLLDRERLATAWALAQAERP
                     NITITTPTIDAFAVGEFFYLMQLQTVMAGALYHVNPFGQPGVEAGKNATYALMGREGY
                     EDLKKELLTEPEDAGKYVNTP"
BASE COUNT         1169 a         1596 c         1774 g         1100 t
ORIGIN      
        1 tcgaccaggc aaggacgtag gcgctacgac cacgaatccg cgattgaacc tagtacgtga
       61 cggtgctccg catctggcac ccgtggctct gaccgtgagt tcgccactca atccagcggt
      121 gcccttgcgc gggccttctc ccgtgcttga catgatgcac tgcggtgcgc acggagtact
      181 tagtgtcaca ttgacaatta ctcttcgttc gcctactatt tggcgcgttg acctgtatgt
      241 gatgacatcg tcatccaatc acaattcctc gccggaggcc gatgtagggc tccggaattc
      301 atgcgggagg caccgcacat ggtgcagcaa acagcacaat cgaaaacaat gtggccagca
      361 tcactggaca ctaacaaggg tggcggaaca gctgtcgccg aacgcccaac caccgcaacg
      421 atgtggttct ccgaaccgca gttggaggtc gcgtcctgca cacctgagga aggtgtcggc
      481 gcttatgacc gcttgaaggc gtacggcgta gggtcccttc gccaggaacg aattccccgc
      541 gagtacttca agatgcaggc ggaagagcgt gacgcacgaa tctttgcgct caaggaagag
      601 ctgggcgccc ggctgacgat cctggggcat cactaccagc gggacgaagt catccagttc
      661 gccgacttcc agggcgactc atacaaactc tcccaggagg cagcaggcgt caccgatgcc
      721 ggattcacgc tcttttgcgg cgtacatttc atggcagaaa gcgccgacat ccttggcggc
      781 ccgaaccagc atgtgatcct cccgaatctc gaggctgggt gttccatggc cgacatggcg
      841 aagccggagg atgtcgaggc ggcctggtcg gtgttggccg ggctcggtat cgacagtgtc
      901 atccctgtca cctacatgaa ctcggctgca tcgatcaagg cgttctgcgg gcgcaacggc
      961 ggcatcgtct gtacctcatc gaacgccatg cgcgtctacg actgggcatt cgagcgtggt
     1021 gagcgcatca tgttcttccc tgatgagcat ctgggccgga acaccgccgt gaagaagggt
     1081 atcccgcagg accagatggt ggtatgggat ccgttccagc ccatgggcgg aaataccgag
     1141 gaggatctcc tcaacgcgag ggtgattctc tggaagggct attgctccgt ccacgcgcgg
     1201 ttctcggtcg agcagatcga gaaggcaagg gtggactacc cggacgtcaa cgtgatcgtc
     1261 cacccggagt gccgtgttga ggtggtacag gcggccgatt gtgacggctc gaccgagtac
     1321 atcatcgata ccatccgcaa tgctcccgct ggaacgacct gggctgtcgg gaccgagatt
     1381 aatctcgtgc gcaggctgca aagccagatg cccgacaaga agatcttctg tctcgatccg
     1441 gtgatctgcc cttgctccac gatgtacagg atccatccgg cgtacatgct ctgggcgctg
     1501 gagcatctgg tacgcggcga gatcgtcaac gagatcaagg tcgatcccca gacgagcgcg
     1561 gacgcgaagg ttgcgctcga tcgcatgctg tctgtgccgt aattccgagg gaatccagga
     1621 tgccgatcga ggttggtgtc gatgcaccgg acttcatgct caagacaagc accggggagg
     1681 acttccggct gagtgacctg cagggaatgt tgcgggtgat gctcatcttc tatcccaagg
     1741 actttacccc cgtttgcacg gaccagctga tccagatacg aaagaacctc aagcatctcc
     1801 gaaaagccgg tatcgagccg gttggtgtca atcccgacga tgcggaaagc cacgagaggt
     1861 tccggaaagc gcatggactc aactttgagc ttctggtgga cgaggaggca gaggtcgcca
     1921 gagcctatgg tgccctgaag cctgacggtg agggcatcct gcggtcggtg atcgttgtgg
     1981 gcaggaatgg caagattgcc tattccaaag aggggaagcc gccctggcag ctcatcctca
     2041 acgcattgcg ccaggtgagc gacgaacccc atccatagcg gtatcgagca ggccccggag
     2101 atatgtctcc ggggcatttc gcttccatac gtccgtatca ctcttatcct tgtttggaca
     2161 actatgaatc gtggcaggcc gtcttgcggg tggcgaccga aagggccggg ctgtctacac
     2221 gtcgacgccc gagatccagt agaacggctc gttgtaatgc cggcggatgg tcgcttcttc
     2281 ctggcggctg agctgggctt cactgtcata gcgcggcgcg gatgccagtt cctcgtgagt
     2341 gaccgcgacc gttactccat cgggagtgat gctgatgcgt tcactgggga tcgcaagacg
     2401 ttggcgaccg aggcccctcc caacgcgaac cacgatatac cggagcagca gctgatcgtc
     2461 gctgataccc ctgtacagca actccggcgg tggattgacg ggctcaggtt cgaagagcaa
     2521 atcttctacg cgcccgataa tctcgccgta ttgagatctg acgagacgat gtcggaggtc
     2581 tcgggggccg cgttggagtc gtgggtccca aatcaggtgt cgaagcgggg agacggcgtc
     2641 ggcaatgacc acggcgcccg taccccgggg gccggtggcc aggagtttcg ccgcctgcga
     2701 cgagatcgta gattccgttt gggtgaatcc aatgtatacg ggcgaattct ccgtgaagat
     2761 ctggtgggcg gtgcgaagta tgtcgatggt ttccgaggtg accgcaatca ggggagaacc
     2821 cgcaacgacc cgctgttcaa cgtaggttga ttcatttcgc tcgaagccga acgcctggaa
     2881 ggtacgggtc agctgacgcg tcggaaggct gcgtagaggt gacacagtgc tgtcgtcgtc
     2941 ctcaccatgc tgacgggatt cgcgtatcgt cgccaggatg gcaccgattg gacccgccgc
     3001 gaggtaggtg gctcgatccg gcaggacgag tgaggcaaga ccttgcagcc agctgcccac
     3061 gacgtcgagc gcgttcttgg ccacgacctc ggagagcacg gcacggtagg gcgtgcgaga
     3121 atcgggatca agcacacgct cgcgaaagat gatcgagatc tggtccgccg gctgcttcag
     3181 tcgacggaga cacatcagtg agctctctgc atcaatgatg tcgtcgaaga gggccatgac
     3241 cgtataggac cgatggagct cctcgtcttc gaattgatcg ataagggaat cgagcatcat
     3301 gcgcggactt caccttcggc cggagcacgg cgtgcagcac tatgaacgat aaagcggcct
     3361 tgtccagggg ggacgaaggg tgtgtcgaag tggcccggtg aaagcggctt gacgtcgagc
     3421 gtacgaagct cggaagcaat gaagtcatcc cgcgatcgga gcaagctgcc cacaaggacc
     3481 tgtaccgcag cgccaatcgg gatcgcgatc agcgcgccag cgggtccccc gagcgtggta
     3541 ccgaccagaa tggcaagaat gaccgtcagt ggcgtcatgc caacggcatt cttcatgact
     3601 cgcgggacaa ggaaggcacc ttcaagttgc tgcatcagga aggccgcgcc aatgacgatc
     3661 agcgcctttt ggggggaatc cgccagcgcg acaagagccg cggcgcttcc accgatgatc
     3721 ggaccgatga acgggatcgc ctcggtgaga cctgcgaaga tcgccagcgc caaccagaag
     3781 cgcaggtcga ttccgtagta gatgatgcca gagatcatcc cgaccgtgag catcagcaag
     3841 agttggccgc gcgtccaccc accgatgcgg tactcgatct cgtcccagat agcatggaca
     3901 cgagtcctcc tggagaaggg gaagagaccg aggacgactc gcttgatcgt cactttctcg
     3961 accatccagt agaaggtgac gatgagcatc gagatggtgg tgagtgtaaa gccgaaaact
     4021 gtattgacta cgctgaatgc ctggtcccgg ccaacttcgg ggctgttccg gatgttgtta
     4081 tagccctgct cgattcgcat caggacacgg aagccggagg tccggacgag gctgctttcg
     4141 ttctgaaggg cctgctggcg caggttatcg aagatttccg ggaccgccgc atcgaacgaa
     4201 gccagttgct tgaccagtag gggcacgagg taatagaggg ccacgcccag gatggtaaag
     4261 aggacgagat agaccaccag gatagcctgg gcacgacgaa gcccggcgcg gcgcagccgg
     4321 aagaccagtg gttcgatgac tgcggcgaag aggatcccga tcgccaggag caacaggatc
     4381 gaacgaatct gccagatcat ccatgctgaa gccacgatcg caatgatggc aatcgtcgag
     4441 atggtcaccc ggatgaggta gggctgctgc gctgtcactg gaactttgcc ttgtcgcgat
     4501 ggctctattg ctgcggagcg tccggtacca atgagttacc ggacgcccat ggatgcgcac
     4561 ttggccggaa ctacggcgtg ttgacgtact ttccggcatc ctctggctcc gtcaggagct
     4621 ccttcttgag atcttcatag ccttcgcgtc ccatgagcgc ataggttgcg tttttcccgg
     4681 cttcaacacc tggttgcccg aatggattta catgatacag tgccccggcc atcaccgtct
     4741 ggagttgcat caagtaaaag aattcgccga ccgcaaaggc atcaattgtg ggtgtcgtga
     4801 ttgtgatatt tggtcgctcg gcctgcgcca gtgcccatgc ggtggcgagt cgctcacggt
     4861 cgagcagctg accgagctcc ccaccctgga agtatgagag ctctgtggct gattcgggtg
     4921 ggctggggat ctccactgtt tcccggtacg ctcccaccgc aatgagctgg atcaacttgt
     4981 cgttcgggcc ctcggtatag agctggatct gggagtgctg gtcgatggca ccgagagcct
     5041 tgattggcgt ctgtccagcg aagacgacct cattgtccgt cgagagcttc ttgcccaggc
     5101 tctcggccca gagctgacgg aaccagtccg acaggccgaa gaggccgtcg gcgtaaggca
     5161 tggtcaccag catcgacttg tcgtggtttg tgtccgcgag gaccgagagc gcggcaagga
     5221 gatacgctgg gttgtcacgg atatcactgc ttgaggcgcg ggcgcgcatg gcggcagccc
     5281 ccgcgagcaa ctggtggata tcaacgccgg caagcgcggc agggataagg cctacggcgc
     5341 tcagtaccgt ctgccgaccg tcgactccag gagggactgg aaacgttcgg tagccttcct
     5401 gatcggccat ctggcgcagg atgccggttt ccggatcggt tgtcgcaacg atctgcctcg
     5461 ccgcttgctc cctgcccaca gcctggatca gggcgtcccg gaccaccagg aaattggcgg
     5521 cagtctcggc cgtttgtccc gacttcgaaa caacattgac cagcgtcctg gagaggtcga
     5581 ctgtttcaag ggtcgcacgg accttttccg ggtccgggtt gtcaagcacg aagaatcgt
//
LOCUS       DASYWM010000195         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_514872, whole genome shotgun sequence.
ACCESSION   DASYWM010000195 DASYWM010000000
VERSION     DASYWM010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514872"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VGR29_08045"
     CDS             <1..845
                     /locus_tag="VGR29_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2073578.1"
                     /translation="PKWLLITTYFLLAVWVIIALFPLYWMVTLSFKNLRLITTGPFFF
                     PWIDFDPVTLGWTDIFQSGQRNRFLTAMRNSLICAIGSGLLSIVLGSMAGYGLTRFSY
                     HFGIWRNRDISFFFLSQLILPPAAVILPLYILFVESGLYDTLLGLTLLYTVVGLPIVI
                     WIMRDQFDSLPVELEQAALVDGHSVIGAFLRIAVPIAAPGMVAAFILVVIFSWNEYLF
                     SAVLTSKGAYTLPLLVATQVSSQGVKFWTMAAIATSTVAPLLVIAIMLERYIVRGLTA
                     GAVK"
     gene            complement(1328..1417)
                     /locus_tag="VGR29_08050"
                     /pseudo
     CDS             complement(1328..1417)
                     /locus_tag="VGR29_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1869..1949
                     /locus_tag="VGR29_08055"
     tRNA            1869..1949
                     /locus_tag="VGR29_08055"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1902..1904,aa:Leu,seq:taa)
     gene            complement(1967..>3512)
                     /gene="mqo"
                     /locus_tag="VGR29_08060"
     CDS             complement(1967..>3512)
                     /gene="mqo"
                     /locus_tag="VGR29_08060"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF003605.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HEV2073579.1"
                     /translation="CTVHAADGTTAHREGPMAETSAEIDVVLIGGGIMSATLGVLLNH
                     VRPDWSIAVYERLDRAAAESSDPWNNAGTGHAALCELNYTPQRADGSIDVTKAVRVNE
                     QFHQSRQFWASLIDQQLLPEPRRFISPVPHLSYVGGEDDVAFLRKRYEALVRIPLFAS
                     LRMSDDVSEIERWVPLMMEGRDLSVPLLFTRSDDGTDVNFGALTKYLIEALVAQGAEV
                     AMRHEVDNLVKQPDGRWSISVTDLSTGERKQVTARFVFIGGGGRAIHLLQRSGIPEAK
                     GYGGFPVGGQWLRSRNPALTDRHKAKVYGKSTVNAPPMAMPHLDLRMIDGKPGLLFGP
                     YAGFSPKFLKHGALTDLPRSIRRDNILTLLTVAKDELPLTLYLIRQALQSNVGKIETL
                     RNFVPTAQEDDWELVAAGQRVQTMKSTRGKRGVLEFGTEVVSTRDGSLAGLLGASPGA
                     STAVSIMLDVMERCFSAEYPGWQGRLRELIPSVGTAIAENPALLEEIQASTDRTLQLK
                     SAVVAT"
BASE COUNT          661 a         1059 c         1028 g          764 t
ORIGIN      
        1 caccgaaatg gctgctgatc accacttatt tcctgcttgc ggtatgggta atcatcgcgc
       61 tgtttccgct ctattggatg gtgaccctct cgttcaagaa tctgcggctg attaccacag
      121 ggccattctt ctttccctgg atagacttcg accccgtaac actggggtgg accgacattt
      181 ttcagagcgg tcagcgtaat cggttcctca ccgccatgcg gaacagcctt atttgtgcaa
      241 tcggctcggg gctgctgtcg atcgtgctcg gctcgatggc tggatatggg cttaccagat
      301 tcagctatca ttttggcatc tggcgaaacc gtgacatatc tttcttcttt ctttcccagt
      361 taatcctacc tccagccgct gtgatcctcc cgctttatat cctgttcgtc gagtccggcc
      421 tttacgacac tctgttgggg ctcacgttgc tgtatacagt tgtcggactc ccaatcgtga
      481 tctggatcat gcgtgaccag ttcgacagtt tgcccgtgga actcgagcaa gctgcactgg
      541 tggacgggca cagcgtgatt ggtgccttcc taaggatcgc cgtgccgatt gctgcaccgg
      601 gaatggtggc ggcgttcatc ctggtcgtga tcttcagttg gaacgagtac ctgttctccg
      661 cagtgttgac ctcgaaaggt gcgtacacgc tgccgctcct tgtcgcgacg caggttagta
      721 gtcaaggtgt gaaattctgg acgatggctg cgatagccac ctcgaccgtt gctccactgc
      781 tggtgattgc cattatgctc gaacgatata ttgtccgcgg tttgacggca ggagctgtga
      841 agtaggtgaa actaaacgga gcaatggtgg aatattcgac ccatatgcag gtgacacgcg
      901 aaacgggggc gcattcttgc gaatgcgccc ccggttacct catgcaggtc aaatattcca
      961 acgttatcgt atcagtgcgc ggaagccact gttcaatgtt gtgcagtccg tgactggata
     1021 atacacgagc aggtggccta ccgacttaga ttgcggagca gcgtagagaa taatcagata
     1081 ggaagtgtac aaactcgcgg ctcagaaatt atcccatcac tcgtttgacg ggataagtta
     1141 taggctgcgc ttttgtgcac ccccaaggcg atcgaactaa gaggacgttt caaaaccatt
     1201 cggtggcccg cgcgtcggcg ttctctgggg cgaggtctag ggtgtcggcg gcgccgcgcg
     1261 ggcggcctcg aggagcaggt cggcgagttc gtacggggcg gtgaccatcg cgtcgcgcca
     1321 tcccgccggg gcggccaccc catggcttgg gcggctcctt cggcaggagg ggggcgatgg
     1381 cttcccagag gtcgtcgggc acaagcggcg tactcatgaa cgaaataatg ccactcccgc
     1441 gaagttctga atcacgcttt ataattccca ttattgcggc agaatggact acctcatgga
     1501 gatggccggt tctctcgatc cgaatatagg gtaccgacgt gcgactcagc atagtcacga
     1561 gcaccgccgg caatgggtgc agaaagggca ggcattgaac ctgcccctgc gaatgcgtga
     1621 cagaaagcag tgcctgacgg atactcagcg gttcctgcga agttctttca gatcttcctg
     1681 ccaaggagaa ggtcgcgggt tcgaatcccg tcttccgctc cattttctgt ggcgacacgg
     1741 gaaacgcgca atgaagggta agtccggtgc ctattggtgc cgggcttgtt tgcatccttc
     1801 tcctcttggc agaggcgtag ccaccccggc tggccttcgc ggtatcatgt ctccgctgtg
     1861 gcggccaagc gggcgtggcg gaacggcaga cgctacggtc ttaaacaccg ttgaggaaac
     1921 tcgtgcgggt tcgaatcccg ccgcccgcac ctctggtcta accgcgttaa gtcgcgacga
     1981 ccgcgctctt cagctgcaac gtcctgtccg tggatgcctg gatttcctcc agcaatgccg
     2041 ggttctcggc aatcgcggtg cccaccgatg ggatgagttc ccgcaatctg ccctgccacc
     2101 ctgggtactc cgccgagaag cagcgctcca tcacgtccag catgatcgaa accgccgtcg
     2161 acgcgccggg tgatgcgcca agcagcccgg ccagtgatcc atcccgggtg ctgacgacct
     2221 ccgtaccgaa ctccagcact ccccgcttcc cgcgagtcga tttcatcgtc tggacgcgct
     2281 ggccggccgc tacgagctcc cagtcatcct cctgggcggt cggcacgaag ttgcgcagcg
     2341 tctcgatctt gccgacgttg gattgcagcg cctgccggat gagatacagg gtcagcggaa
     2401 gctcatcctt cgccactgtc aacagggtca ggatgttgtc ccgcctgatc gatcgaggca
     2461 gatcggtgag cgcaccatgc ttcaggaatt tcggtgagaa cccggcatac ggaccgaaca
     2521 gcaaaccggg tttgccatcg atcatccgga ggtcgaggtg aggcatcgcc atcggcggcg
     2581 cgttgaccgt tgacttgccg tagaccttcg ccttgtgcct gtccgtcagc gccgggttcc
     2641 tgctgcgcag ccactgtcct cctaccggga aaccgccgta gcccttcgcc tccgggatgc
     2701 ctgacctctg caacagatgg atggcccgcc ctccgccgcc gataaagaca aagcgggccg
     2761 tcacctgttt ccgttccccg gtcgaaagat cggtcacgct gatagaccag cgaccatcag
     2821 gctgcttcac gaggttgtct acctcgtggc gcatcgccac ctcggccccc tgggcaacga
     2881 gcgcctcgat caggtacttc gtcagcgcgc cgaagttcac gtccgtgccg tcatcgctgc
     2941 gggtgaagag cagtggaacg gagagatccc ggccctccat catcaacggc acccaccgct
     3001 cgatctcgct gacgtcgtcg gacatccgca ggctggcaaa gagcggtatc cggacaagcg
     3061 cctcgtagcg cttccggaga aacgccacat cgtcttctcc acctacatag ctgagatggg
     3121 gtaccggact tatgaaccgc cggggctccg gcaggagctg ttgatcgatg agggaggccc
     3181 agaactggcg tgactgatgg aactgctcgt tgacccgcac ggccttggta acatcgatgc
     3241 tgccgtccgc ccgctgcggg gtgtagttga gctcgcacaa tgcggcatga ccagttccgg
     3301 cattgttcca tggatccgag ctttcggccg ccgcccggtc caggcgctcg tataccgcaa
     3361 tcgaccaatc aggccggaca tggttgagca gcacgccgag cgtggcgctc atgatcccgc
     3421 cgccgatcag gacaacatcg atctccgcgg atgtctctgc catgggccct tctctgtgcg
     3481 cagtggtgcc gtcagccgca tgaaccgtgc at
//
LOCUS       DASYWM010000196         2842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_552394, whole genome shotgun sequence.
ACCESSION   DASYWM010000196 DASYWM010000000
VERSION     DASYWM010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552394"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..135
                     /locus_tag="VGR29_08065"
     tRNA            63..135
                     /locus_tag="VGR29_08065"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Ala,seq:ggc)
     gene            274..2784
                     /gene="leuS"
                     /locus_tag="VGR29_08070"
     CDS             274..2784
                     /gene="leuS"
                     /locus_tag="VGR29_08070"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV2073580.1"
                     /translation="MTTTSDSRPEQHVHGADAHRYDHREIEPRWRQAWQDKAIYKVND
                     DSERPKWFSLTMYPYPSGILHVGHWYAFAVPDVFARLQRMRGYNVMFPMGFDAFGLPA
                     ENAAIRNNIHPSTWNNTNIPQMKRQYDLMGAMIDWSREVNTSDPEYYQWNQWIFLKML
                     EKGLAYRKDGAVWWCPNDQTVLANEQVLEGNICERCGADVYKRDLEQWYFRITEYAEE
                     LLNELDNLDWPERVKTMQRNWIGRSEGARLQFELETGDALEVFTTRPDTVFGATFMVM
                     APEHPLVEKITTDEQREQVEAYVTKARNETEIERLSLDEAKKKTGVFTGAYAVNPVND
                     ERIPVWIADYVLMSYGTGAIMAVPAHDERDFAFARAFDLPIQVVIQPEDQKALQPAAM
                     DEAYVGPGRMVNSGQFDGTPVPESVDTVITWLDEQGRGKAEVTWRLRDWLISRQRYWG
                     TPIPIVYCDGCGTVPVPLEQLPVELPLDAEFKPTGQSPLMTHERFLNTTCPSCGGPVR
                     RETDTMDTFMDSSWYWFRYTSPHYMDAPFDPETAKKWTPVDLYCGGIEHAILHLLYAR
                     FFTKVLRDMGLVDHGEPFTRLRNQGMILSAEGVKMSKSRGTQIGPDDLVEEHGADALR
                     LHLMFLGPWEQGGPWNDRGITGMERFIRRAFQIVSGVGDARLDGDAPEADIRALRSLT
                     HRTIRRVTEDIDTFSFNTMIAALIEFTNELMRLRDQPVARTAAWREAAETLTLLMAPS
                     TPYVAEEMWSRLGNAFSVHQQAWPAFDEVLASVDTIEIPVQINGKVRDKIVVGAGLST
                     DDLLAAAKASDKIQELMVGKTIVKEISVPGRLVNIVVR"
BASE COUNT          600 a          821 c          915 g          506 t
ORIGIN      
        1 ggaatcctcc gttatcaggg agccaagcgg accaaaagac ggttcgtgat cagatgccac
       61 gcggggccgt agctcagctg ggagagcacc acactggcag tgtgggggtc aggggttcga
      121 gtcccctcgg ctccacccga atgcttctga atcacgagtg cccgacctgg tcaagcaggg
      181 tggtaccgcg gaagcgccaa gacgccttcg tccctgagag gggatggagg cgtctttgtc
      241 gttaatgagc gaaatcgaga aaaggacaag ctgatgacca cgacaagtga ttccaggcca
      301 gagcaacacg tccacggagc tgatgcacac agatacgacc acagggagat cgagccaagg
      361 tggcgtcagg catggcaaga caaggcaatc tacaaggtta acgacgacag cgaacggccg
      421 aaatggttct cactgacgat gtacccgtat ccctccggca tcctgcatgt cggccactgg
      481 tacgcgttcg ccgtgccaga cgtattcgcc cggctgcaac ggatgcgtgg atacaacgtc
      541 atgtttccga tgggcttcga cgcctttggc ctgcccgccg agaacgccgc aatcaggaat
      601 aatatccatc cctctacctg gaacaacacc aacatcccac agatgaagcg ccaatacgac
      661 ttgatgggcg cgatgatcga ctggtcgcgg gaggtgaata cctcggatcc ggaatactat
      721 cagtggaacc agtggatctt cctgaagatg ctggagaagg ggcttgccta ccgcaaggac
      781 ggcgcagtct ggtggtgccc gaatgaccag acggtgctcg ccaacgagca ggttctcgaa
      841 ggcaatatct gcgagcgatg cggcgcggac gtttacaagc gtgacctgga gcagtggtac
      901 ttccggatca ccgaatacgc cgaagagctg ttgaacgagc tggacaacct ggactggccc
      961 gagcgcgtga agacgatgca gcgcaactgg atcggccgct ccgagggcgc ccgcctgcag
     1021 ttcgagctcg aaacgggcga cgcactggag gtcttcacga cccggcccga cacagtcttt
     1081 ggcgccacct tcatggtcat ggcgccggag cacccactgg tggagaaaat caccaccgac
     1141 gagcagcgcg agcaagtcga ggcatacgtc acgaaggcac gaaacgagac ggagatcgag
     1201 cgcctgtcgc tggacgaggc gaagaagaag accggcgtct tcactggggc ctatgccgtc
     1261 aacccggtga acgatgagcg cataccggtg tggatcgctg attacgtgct gatgtcctac
     1321 gggacgggcg cgatcatggc ggtcccggcg cacgacgagc gcgactttgc gttcgccagg
     1381 gcgttcgatt tgccgattca ggtcgtcatc cagccagagg accagaaagc gctccagcca
     1441 gcggcgatgg acgaggcgta tgtgggtcca gggaggatgg tcaactccgg ccagttcgac
     1501 ggcacgccgg tgccggagag cgtcgacacg gtgatcacct ggctggacga gcagggccgc
     1561 ggtaaggcgg aggtcacctg gcgcctgcgc gactggctga tttcccgcca gcgctactgg
     1621 ggcacgccga ttcccattgt ctactgcgac ggatgcggca cggtccccgt tccgctggag
     1681 cagttgccgg tcgagcttcc gctcgacgcc gagttcaagc cgaccggaca gagtccgctg
     1741 atgacccatg aacggttcct gaacaccacg tgcccatcgt gcggcgggcc tgtccggcgt
     1801 gaaaccgaca cgatggatac cttcatggac tcctcgtggt attggttccg ctataccagc
     1861 ccgcattaca tggatgcgcc gttcgacccg gagaccgcga agaagtggac gccagtagac
     1921 ctgtactgcg gcgggatcga acatgcgatc ctgcatctgc tctacgcccg gttcttcacc
     1981 aaggtactgc gggatatggg gctggtggac cacggcgagc cgttcacccg tctgcgcaat
     2041 cagggcatga tcctctcggc cgaaggcgtc aagatgagca agagccgtgg cacccagatc
     2101 ggccctgatg atctcgtgga ggagcacggg gctgacgctc tgcgactgca cctgatgttc
     2161 ctgggtccct gggagcaagg tggcccctgg aatgatcgcg ggatcaccgg catggagcgg
     2221 ttcatccgcc gtgccttcca gattgtgtcg ggagtgggcg atgcgcgtct ggatggggac
     2281 gccccggagg ccgacattcg cgccctgcgg tccctgaccc atcgcacgat taggcgggtg
     2341 accgaggaca tcgacacgtt ctccttcaat accatgatcg cggcactgat tgagttcacc
     2401 aacgaactga tgaggctgcg ggatcagcca gtggcaagaa ccgccgcctg gcgcgaggcc
     2461 gcggagacgc tgacgctcct gatggcaccc agcactccgt atgtcgccga agagatgtgg
     2521 tcgcgtttgg ggaatgcgtt ctcggtgcat cagcaagcgt ggccggcgtt cgacgaagta
     2581 ctggcttcgg tggatacgat agagatcccg gtgcagatca acggcaaggt tcgtgacaag
     2641 atcgtcgtag gagcgggact ttcgacggac gacctgttgg ctgctgccaa ggcaagcgac
     2701 aagattcagg agttgatggt cggcaagacc atcgtcaagg agatctcggt cccgggtcgg
     2761 ttggtgaata tcgtcgtccg ataggattga ttccctctaa cagtcgcagt aatggccggc
     2821 ctccataggc cgtctattac tg
//
LOCUS       DASYWM010000197         4505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_590363, whole genome shotgun sequence.
ACCESSION   DASYWM010000197 DASYWM010000000
VERSION     DASYWM010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590363"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2087
                     /locus_tag="VGR29_08075"
     CDS             <1..2087
                     /locus_tag="VGR29_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073581.1"
                     /translation="ASRTLRVPLFPEIGEHWDMHYRAIAAEAPKDAKDVLPEAVDVVE
                     GGIRLGTIRYPAVLMEWIDGPTVLQGADRAARAGNTVVLRAFAEALKDLAGTLRRGRM
                     VHGDLAADNLLMRSTGDLVCVDLDGLSWLGSPAAVSREVSVAYRHPGRSTSSPQRDAF
                     ALLVQYVSLAVLADDPDLRRSYGDPVSTHGGAILFSSWDLRDPLRSRTFADVRDRVGP
                     TTAALVDDLQRACLGDASEAPSILSAVLQLPNMIQNVPVDAAEETTTWDLSRVIDRLK
                     THYPEQQETRRLPAVPAQPPASESQGFAVSGTVTEDRERLREAIAQGNDAEVIQWAVR
                     LADDPVAQLYKLDVERVLAVGYRSRIEQAAAQGQDETVVLLAEQAESRQLPLDATSRR
                     AVRNARERIEVRGKLEAALTSNDRAALADLAVSGELVVLGDADRASLQRVLQAIEWPS
                     LQRALETDEDSLILEAFDEELFVDGSGVPTHVRSRLMLARERVAWISDVRTALKRRNA
                     RELARLYASEPEGGLDRLRASERTRASRMLRQQAALDALQGAMQTGNDISIVEALHAI
                     ERTGARIEDRFAWTAVQGVMRRATLIESIVAAANADPADDRQLAHLLPAAKTMGLMHD
                     PLLQGELAWDRLQEVVVRGAALRRIRRAIAADDDRAIRKAAFPDVTAALQQLTTEERA
                     RVDAALQRRRAG"
     gene            complement(2102..2314)
                     /locus_tag="VGR29_08080"
     CDS             complement(2102..2314)
                     /locus_tag="VGR29_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073582.1"
                     /translation="MKMGVFTVPFAHRPFGEALDVIKAAGCEAVETGTGGAPGNAHCD
                     LDYLVENNQAPICMQPRANDARGCMC"
     gene            2532..4106
                     /locus_tag="VGR29_08085"
     CDS             2532..4106
                     /locus_tag="VGR29_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEV2073583.1"
                     /translation="MRSIDRDPGRGSRLIVANRYEIDVDNPLGRGGMAMVYVGHDLKT
                     RRKVAVKTLRPEYQRDPESRQRFRREARMMALVSHPNLVTIYDLHDETSGSWMVMEYV
                     EGQNLRDVLEDEGPLHPEAVMVILSQVAGALAHMHSRHLVHLDVKPQNLIRTPDGTIK
                     LIDFGLAQIAGVPQEMIGGAAFGTVAYLAPEQASGGMVDAATDVYSLGCVMYELLTGQ
                     TPFVADGPDHKRQLIDAHLNSRPPAPSMVRPELGLPSWIDDVLGWALAKNRTDRFHDV
                     ETFVQVFEAGLDGETIRHPAQQTLAFEPQADREVERTGIFRRRPGPETHVNHPVEVHG
                     NDGAATIPKVRPVNESTARQLYRRGGRIARRSRRFRRVLWRLAAILLVANLLLALILM
                     AKDGPAVLVERFLSFAPGTSTEVVTDVLNLREAPGTTSPVLLVLGQGEEVKITGLSEL
                     DEQGRWWPVEVDRGGETVTGWVWEGGLQPNAWTGRLSWMQDIVDGVNDTKNSIQNGVE
                     RVLDILPGVMIQPRFL"
BASE COUNT          821 a         1286 c         1534 g          864 t
ORIGIN      
        1 gggcatcccg taccctgcgt gtgcccctgt tccctgagat cggtgagcat tgggacatgc
       61 actaccgtgc cattgcagcc gaggctccga aggatgcaaa ggatgtgttg ccggaggccg
      121 tcgatgtggt ggagggtggt atcaggctcg gaaccatacg atacccggcg gtgttgatgg
      181 agtggatcga tggtcccact gttcttcagg gtgccgatcg cgcggcaagg gccgggaaca
      241 ctgttgtgct gcgcgcgttc gccgaagcgc tgaaggatct ggctggaact ctcaggcgag
      301 ggcggatggt gcatggggac ctcgccgccg acaaccttct tatgcgatcc actggtgatc
      361 tcgtctgtgt cgatttggac ggtttgtcct ggctgggttc gccagccgcc gtgtcgcggg
      421 aggtctcggt ggcctaccgg cacccggggc gaagcacctc cagcccgcag cgtgacgcct
      481 tcgcgctgct cgtgcagtac gtctcactgg ctgtgctggc agacgatcct gacctacggc
      541 gctcctacgg tgatccggtc tcaactcacg gcggtgcgat cctcttttcc tcctgggatc
      601 tcagggaccc gctgcgatcg cggacattcg ccgatgtgcg ggaccgcgtc ggcccgacaa
      661 cagctgcgct tgtcgacgat ctgcagcggg cctgcctggg tgacgcttca gaagcaccga
      721 gtatcctgag tgcggtcctc caacttccca acatgatcca gaatgttcct gttgatgctg
      781 ctgaggaaac gacgacgtgg gatttgtccc gggtaataga ccggctgaag acgcactacc
      841 ctgagcagca ggagacgcga cgactcccgg cggtcccggc acaaccaccc gcgtccgagt
      901 cgcaaggatt cgcggtatcc gggacggtga ccgaggacag ggagcggttg cgggaagcga
      961 ttgcgcaggg caatgatgcc gaggtgatcc aatgggcggt gcgactggca gatgatccgg
     1021 tggcgcaact ctacaagctg gatgtcgagc gcgtcctggc agtcgggtat cggagccgga
     1081 tcgaacaggc ggcggcacag gggcaggacg aaaccgttgt gctccttgcg gagcaagccg
     1141 agtcgcgcca gttgccactg gatgcgacat cgcgacgcgc ggtacggaac gcgagagaac
     1201 ggatcgaggt ccgcggcaag cttgaagcgg cactcaccag caacgaccgg gccgccttgg
     1261 cggatctggc ggtttccggt gaactggtcg tcctcggaga tgccgacagg gcatcgctgc
     1321 aacgggtgtt gcaggcaatc gaatggccga gcctgcaacg cgccctggag accgacgaag
     1381 attcactgat cctcgaagcc ttcgacgagg agttgtttgt ggatggatcg ggcgttccca
     1441 cccatgtccg atcgcgcctg atgctcgccc gcgaacgagt tgcctggatc tccgacgttc
     1501 ggacggcgct gaagcggcgc aatgcgcggg agctggcacg gttgtatgcg tccgagccgg
     1561 aaggcgggtt ggatcgtttg cgtgcatcgg agcgtacgcg ggcgagccgt atgctgcggc
     1621 agcaggcggc gctggacgcg ctccagggag caatgcagac ggggaacgat atctcgatcg
     1681 tggaggcgct tcatgcgatc gagcggactg gcgcacggat cgaggacagg tttgcctgga
     1741 cggcagtgca gggcgtgatg cggcgtgcaa cgctcatcga aagcatcgtg gcggccgcga
     1801 atgcggatcc ggctgatgac cgacagttgg cgcatctgct gccagccgcg aagacgatgg
     1861 ggttgatgca cgatccgttg ttgcagggcg aactggcgtg ggaccggctg caggaggtgg
     1921 tcgtgcgcgg tgccgcgctc aggcgtatcc ggcgcgcaat cgcggcggat gacgatcgcg
     1981 caatacggaa ggcggccttc cccgatgtga cagcagcgct gcagcaattg accaccgagg
     2041 aacgagcccg ggttgatgca gccctgcagc gacgcagggc gggatagcga ctgatcggag
     2101 atcagcacat gcaaccccgg gcatcgttgg cccggggttg catgcatatt ggggcctgat
     2161 tgttctcgac caggtaatcg aggtcgcagt gggcgttgcc cggggcacca ccggtgccag
     2221 tttcgacggc ctcgcagcca gccgccttga tgacatcgag cgcttcccca aaggggcggt
     2281 gtgcaaacgg gacggtaaag acgcccatct tcatggtgtg tctctccccc tgtcttgtgg
     2341 tcggacgatc cgtcgtcgcg atccattctt accggtcatt ttccgcttga tgccgccatc
     2401 cttgcggtct tagtacccca tccttccggg tggttggtgc cggtccttga gacggcgttt
     2461 ggtacattgg acggagcgtc acgacgatag agcgtccccc tagcctcgct tgagagagtc
     2521 cggattattc catgaggtcc attgatcgcg atccaggacg gggatcgcga ctgattgtcg
     2581 ccaaccggta tgagattgat gtcgataacc ctctgggtcg tggcggcatg gccatggtgt
     2641 atgtgggaca cgacctgaag acgcgccgga aggtcgccgt gaagacgttg cggcccgaat
     2701 atcagcgaga tccggaatca aggcagcgtt tccggagaga ggcccggatg atggcactgg
     2761 tgtcccatcc gaacctggtg accatctacg accttcacga cgagacgagc ggatcatgga
     2821 tggtcatgga gtacgtcgag gggcagaacc tgcgcgacgt gctggaggac gaaggcccgc
     2881 tgcacccgga agccgtgatg gtgatcctga gccaagtcgc cggtgcgctt gctcacatgc
     2941 attcccggca tctggtgcac ctcgatgtca agccccagaa tctcatacgc acgcctgacg
     3001 gaacgatcaa gctgatcgac ttcggtcttg cccagatcgc tggtgtgccg caggagatga
     3061 tcggcggtgc tgccttcggg accgtcgcct atcttgctcc cgagcaggca agtggcggca
     3121 tggttgacgc cgccactgat gtctattccc tggggtgcgt gatgtacgag ctgcttacgg
     3181 ggcaaacgcc tttcgtcgcg gatggccctg atcacaagcg gcagttgatc gacgcgcatc
     3241 tcaactcacg gccgccggca ccatcaatgg tccgcccaga gcttggtcta ccctcctgga
     3301 tcgacgatgt tctggggtgg gccctcgcga agaaccggac ggaccggttc cacgatgttg
     3361 agacattcgt gcaggtgttc gaggccgggt tggatggcga gaccatccgg catccggcac
     3421 agcagacgct ggcgttcgaa ccgcaagccg accgtgaagt ggaacgaacg ggaatctttc
     3481 gacgaagacc gggacctgaa actcatgtga atcacccggt tgaggtccat gggaacgatg
     3541 gggcagcgac gatccccaag gtgcggcccg tcaacgagag caccgcgagg cagctctatc
     3601 gcaggggagg tcggatcgcc cggcggtcac gccgcttccg gcgtgttctc tggaggttgg
     3661 ccgcgatact gttggttgcg aaccttctgc tggcgttgat cctgatggcg aaggatggcc
     3721 cggccgttct cgtcgagcgc ttcctctcgt tcgcgccggg taccagtacc gaggtagtca
     3781 ctgacgtgct gaacctgcgt gaggcccctg gtacgacatc gccggtcctc ctggttcttg
     3841 gccaagggga ggaagtgaag attaccggtc tctcggaact ggatgagcag gggcgttggt
     3901 ggccggtgga agtggaccgg ggcggcgaga ctgtgacggg ctgggtttgg gaaggtgggt
     3961 tgcaaccgaa cgcctggacc ggccggctct catggatgca ggacatcgtc gatggtgtga
     4021 acgacacgaa gaattccatc cagaatggcg ttgagcgggt gctcgatatt ctgcctggtg
     4081 tgatgatcca accgcgattc ctttgacacc ttttgacatc ccggtcccaa cggtaacgag
     4141 gccgctccca tgagattcct cggcggggca gcaagagcac cgcgtgattg ttaatagatt
     4201 ctgagggtcc ggggctctca gcaaggttgg cgatgtgcgg gctgttagga ggatccgttc
     4261 gacaggccca gggcaggcta gtgctgcgtt cgtggccgcg aacaggcagc atgcaccgca
     4321 agtcctcggg tgagccctca ggcgaatagc gggagcgacc ccttcgctac agacgacaga
     4381 ggcgacagac atgcaacctc tctgaaaacc gctcttgaga gcggcggata ctaccccacg
     4441 ggatgggtgt ttcttcgcgc cgattcctta ctatcgcgtc aggtcgtgtg ccggtggcca
     4501 atccg
//
LOCUS       DASYWM010000198        10552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_646331, whole genome shotgun sequence.
ACCESSION   DASYWM010000198 DASYWM010000000
VERSION     DASYWM010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646331"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VGR29_08090"
     CDS             <1..363
                     /locus_tag="VGR29_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2073584.1"
                     /translation="KGGSIMTMTYLGSDRVFPKYNVMGVAKAALESTMRYLAADLGEQ
                     GIRVNAISAGPIKTASARGIPGFSAMQEEAARRSPLGDIFDTEHVAGTAVFLASDASA
                     GITGDVIFVDGGYHAMGL"
     gene            complement(822..1736)
                     /locus_tag="VGR29_08095"
     CDS             complement(822..1736)
                     /locus_tag="VGR29_08095"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEV2073585.1"
                     /translation="MRNLPFDLPGRFYKGNLHSHSNNSDGALAPEDVVNTYRDHGYDF
                     ISITDHFMNRFNWPVTDTTHFRDEQFTTLLGAELHGGGMENGELWHVVANGLPEDFAD
                     QHPNEDGAEIAARAIGEGAFVTIGHPAWNGVTEKDVDKLHDFDAIEIYNHGHTNDCDR
                     GNGWMIADSLATRGLRFSTTAADDAHFKLRPDTFGGWVQVKSPSLDPDSILKALKDGR
                     YYASTGPEIHNVEFTESEILIECSPAIAVFVGGRGSIKAYERGDGLTRVRIPLSPFLN
                     SFCRVTVLDEKGFRAWTSPIWLDELNLT"
     gene            complement(1853..2668)
                     /locus_tag="VGR29_08100"
     CDS             complement(1853..2668)
                     /locus_tag="VGR29_08100"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2073586.1"
                     /translation="MPNASKKRVLVTGSGGLIGGLLINEWQKSDSYEPVGLGRKPGPH
                     ADIVANITDLDAVEAACRGMDAVVHLAATISLSSSWDAVLNSNLIGTYNVFEAARRAG
                     VDQVIFASSNHSIGTYETENVPELWDLDNGRTWDHTAEIRPDSLYGVSKVYGEGMARY
                     YVDHHGLRAACLRIGAVRSPQDPSHPSNLWKPERDDEENMRQQRRRMRAVWLSERDCV
                     QLIEKSLETDRDWVLVYGISDNPRKIWDINHAREVLGYAPQDSAPEEIFPGER"
     gene            complement(2772..3845)
                     /locus_tag="VGR29_08105"
     CDS             complement(2772..3845)
                     /locus_tag="VGR29_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2073587.1"
                     /translation="MERLRVAIIGTGGVAGSHMNAWDDCKDRLNRVAAVDIDEERVSA
                     FANKHGIPHSYADVGEMLREQQPDLVHIATPPSLHAQQILQCLEAGAHVLCEKPLVGS
                     LRQLDLIAEAEQRSGRFCCSVVQWRFGSAGQQIRRLIDSGTFGRHTVSICATTWYRDD
                     AYYAVPWRGTWASELGGVSMGQAIHLIDLMLWLNTPWRDVTAITETLDHDIEVEDASL
                     AMVRFEDRSVASVISSVVSPRQETRLRLDFQRATAEVVALYRHRNANWTITPVAGTEL
                     PAGTWPPETDLESNHGAQLAMMLDVLEAGRRPPAGTDDIRPTIEFLSALYKSGMTGAP
                     VERGSIGPGDPFYDHVSGRVNPA"
     gene            complement(3897..4640)
                     /locus_tag="VGR29_08110"
     CDS             complement(3897..4640)
                     /locus_tag="VGR29_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073588.1"
                     /translation="MAWQHLGLPQFPSAVGFTWLRVYDSVAPDGVRGGSPHVHLASTK
                     AYVTVAGHGEVHTLGPDGFGTLDLRPGAVVWFEPGIIHRLVNTSSDLEIFVVMQNNGL
                     PEAGDAVLTVPEVILTDPDTYRSAAGPEVASAVKWPVDAAVLRNLAVEGFTAMLDADD
                     TDRLARYERFLKNAVSLRRDPVSEWRQLWQERPLAEAQRTGERLDEIEVGAITALMQA
                     RVASYMPKAEPAVGMCGQLDTYLPEGWTH"
     gene            complement(4643..5491)
                     /locus_tag="VGR29_08115"
     CDS             complement(4643..5491)
                     /locus_tag="VGR29_08115"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HEV2073589.1"
                     /translation="MLISHDVGKTISCFPIDKRNSSWILDYTTAPKPFMHPVQTPAGH
                     LLSLLEPADHLWHRGLWFTIKFVNGDNFWEERPPFGTQCVVGIPEITHGSPEDVELRL
                     ALDWIAPNGGQRMIAERRTIRSRATDDAFVIDWGTVLTAERDITLDRTPYTTWGGYGG
                     LSFRGSRSWLVDRFLLPDGVADSRPAGQTGAWCEMSGPLDGGANLSAGLAMFDHPDNP
                     RYPAPWYGGGAGSGNFLNAALLFHKPMKLQKGEVLALRHRVLIHDGIWDAERLQAEHQ
                     RWLQDK"
     gene            complement(5815..6990)
                     /locus_tag="VGR29_08120"
     CDS             complement(5815..6990)
                     /locus_tag="VGR29_08120"
                     /inference="COORDINATES: protein motif:HMM:NF017951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV2073590.1"
                     /translation="MALASTSLNLPTVSGEVEAFSARPAADYGTGFVEPRTRVAFAAA
                     HVVPDPLADINPTTDVALDWEATLAYRRYLWSMGLRVAEAMDTAQRGMGLDWETTREL
                     IRRSVTEGKAVGGQLASGAGTDQLRLGPELTLDDVTAAYEEQFAYVEGLGGRIIMMAS
                     RALAACARSPDDYASVYGRILEQASEPVIIHWLGDMFDPALAGYWGNDNLDQAMDTAV
                     AIITQHADKVDGVKVSLLDDQREVDMRRRLPESVRMYTGDDFNYPDLIKGDEQGYSNA
                     LLGIFDPIAPIAGAAFAALDAGDMDRYDELFAPTVPLSRHIFKAPTRFYKTGVVFLAW
                     LNGHQDHFRMVNAQESCRSVVHLAELFRLADAARLFRDPERSTRRMRSFLEVAGVAQ"
     gene            7593..8579
                     /locus_tag="VGR29_08125"
     CDS             7593..8579
                     /locus_tag="VGR29_08125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2073591.1"
                     /translation="MKFGVFLPTFLFPGTDAGHAERLRRFARKAEELGFDSLWITDHI
                     VTARGLYAVSWLDSLTTLAHVSAITERARLGTSVLILPVRQPVVLAKEIATLQHLSGG
                     RYNFGVGTGWYGPEFESAGVHKSERGVRTDEVLDAVNQLLREPNVTFSGRYYHLNDVT
                     VEPLSTVPPVWVAGGRQLVHAASPEKSAMHPRVLKRIARSDAWIARPTCPPDLIAVDM
                     AEIRTARQEAGLDGEPFTMAHENFVYIVEEGSPEDIVREQQEKYFRLTGSARPWDYIE
                     AVYLNGTVDRIQSMVDARRSVGIEYMMLHTLTDDLSQLELIARHIVQPFANA"
     gene            8797..9888
                     /locus_tag="VGR29_08130"
     CDS             8797..9888
                     /locus_tag="VGR29_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2073592.1"
                     /translation="MRKSKLSFGILGCGVIGPHHAAAITALSDEAHLVAVADSEPERA
                     QQLAQQYGVPAYQSMQDLVARDDIDVISICTPSGMHAEHAVAAMEAGKHVVVEKPVDI
                     TLPAVDKLIAVQRETGRKATVISQHRFDPASQVVHEAAHDGRFGRLTMGNALVRWWRS
                     QAYYDSGAWRGTWAMDGGGVLMNQSIHSVDLLLWMMGPVAEVSAYTGLLAHERLEVED
                     TAVAVLQFASGALGIIEGTTAAYPGLSARLEISGDRGSATIDDDRLAYFHSAADGEQA
                     DSYGSRGEGNQADLVLPSEEAHTSAGSNPGNLSTRHREQISDFIEAIHQEREPLVTLE
                     EGRKAVAVILAIYESARTGRPTPVEGARV"
     gene            10085..>10552
                     /locus_tag="VGR29_08135"
     CDS             10085..>10552
                     /locus_tag="VGR29_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073593.1"
                     /translation="MSSNDQTNVRAFDRRTLVKGVAGTAIGASALPTAMAHASTTTRG
                     SSMNRIQDGEPITLVYFNARGGEAVERALVERYMEENPNITIEYLSTTAIGGPSDTDT
                     IANLIFNIQADTTVDVAKVEVQRTPLELMATGSALELTEIDAEAVRARSDELLN"
BASE COUNT         2164 a         3332 c         3073 g         1983 t
ORIGIN      
        1 aagggcggca gcatcatgac gatgacctac ctcggctccg accgcgtctt ccccaagtac
       61 aacgtgatgg gtgtcgccaa ggcggcgctg gaatcgacca tgcgctacct cgccgccgat
      121 ctcggggagc agggtattcg ggtcaatgcc atctccgccg gcccgatcaa gaccgcatcg
      181 gcacgcggca tcccaggctt ctccgccatg caggaggagg ccgccaggcg ctctcccctg
      241 ggtgatatct tcgacacgga gcatgtggcc ggcaccgccg tcttcctggc gagcgatgcc
      301 tcggcgggca tcaccgggga cgtcatcttc gtcgacggtg gctaccacgc gatggggctc
      361 tagtcgaccg taattgggca agggcacaag gggcagccga tcggctgccc cttgtgtatc
      421 tcctaactag ccagactgca gcactgatgc tttccccgtt tggatccagc cgccccgcaa
      481 caatcatcgg ccactcccgg gactgctccg gtcatcggcc ggccctcccg gcgccttggg
      541 gtttgtctct tgggccacaa cacctaaaac cgcaagcgcc atcactccca gcacagactc
      601 atacttgcca caagttgaac caatcgtttc atctcacttc tcccttcgag ccatcgtcca
      661 aacagccctt tgcatccagg agggtcgtgg ggtgcacgtt cgcaagtgtg gagaggtggc
      721 gaagagatgg gtatcgcatg aacgtgtgat tttgcgatcc caagtacatc aagagggggg
      781 cggactgata gcccgccccc ctcttcctgc cgatcgatcc cctaagtcag gttcaactca
      841 tcgagccaga tcggacttgt ccaggcccga aaaccctttt cgtccaacac cgtcacccgg
      901 cagaaggagt tcaggaaggg agagagcgga atccgcaccc tcgtcaggcc atcgccacgc
      961 tcgtacgcct taattgaacc gcgaccgcca acgaacacag caatcgcggg ggaacactcg
     1021 atgaggatct ccgactcggt gaactcgaca ttgtggatct ctggacccgt gctggcgtag
     1081 tatcgaccgt ccttgagcgc cttaagaatg gaatccggat caagagatgg cgacttcacc
     1141 tgcacccagc ccccgaacgt gtcaggccgg agcttgaagt gggcatcgtc cgccgcggtg
     1201 gtggagaagc gcaggccgcg ggtggcgagc gagtcggcga tcatccagcc gttgccacga
     1261 tcgcagtcgt tggtgtggcc gtggttgtag atttcgatgg catcaaaatc gtggagcttg
     1321 tcgacatcct tctcggtgac gccgttccac gccggatgcc cgatggtgac aaacgcgcct
     1381 tcaccgatcg cccgcgcggc gatctccgca ccatcctcgt tgggatgttg atctgcaaag
     1441 tcctccggca gcccgtttgc gacaacgtgc cagagctctc cgttctccat gccgccacca
     1501 tgaagctccg ctccgagcag tgttgtgaat tgctcgtcgc gaaaatgcgt ggtatccgtc
     1561 acaggccagt tgaaccggtt catgaaatga tcggtgatcg agatgaagtc gtacccgtgg
     1621 tcgcggtacg tattgacgac atcctccggt gcgagggcac cgtccgaatt gttggaatgc
     1681 gaatgcaggt tgcccttgta gaaccggcca ggaagatcga acgggaggtt acgcacgcgg
     1741 aaggtcccct tcgtgggtga tggatgcaga acgggcggac acgactgctc acgtccgccc
     1801 gttaccttac ccgatataag gcgagaaagg ggctggcatc ttggagaaac ttctaccgct
     1861 cgcccggaaa gatctcctcc ggcgcgctgt cctgcggtgc gtatcccagg acctcgcgcg
     1921 catgattgat gtcccagatt ttccttgggt tgtcagagat cccatacacc agcacccagt
     1981 cacggtctgt ctcaagcgac ttctcgatca actggacgca gtcccgctcg ctgagccaga
     2041 ccgcccgcat cctccggcgc tgctgccgca tgttttcctc gtcgtcgcgc tcgggcttcc
     2101 acaggtttga aggatgggaa ggatcctgcg gcgagcgaac cgcaccgatc cgcaagcaag
     2161 ctgccctgag accatgatga tcgacgtagt acctcgccat cccttccccg tacaccttgg
     2221 agacgccgta gagcgaatcc ggacgaatct cggcagtgtg atcccaggtc cggccgttat
     2281 ccagatccca aagctcggga acgttctccg tctcgtatgt tccgatggag tggttggacg
     2341 atgcgaagat cacctgatcg acaccggcgc gacgggcggc ttcgaagacg ttgtacgtac
     2401 cgatcaaatt gctgttgagc acggcatccc aggaggaact caacgaaatg gtcgcggcaa
     2461 gatgcacgac cgcgtccata cctcggcacg ccgcctcaac cgcgtcaaga tccgtgatgt
     2521 tcgccacgat atcggcgtgc ggtcccggct tccgccccag ccccactggc tcgtacgagt
     2581 cagatttctg ccattcattg atcagcaagc ccccgatcag cccacctgat ccggtcacca
     2641 acacacgctt tttcgatgca ttcggcatag tctgtcccgc tgtcagtcaa tcagcgaaat
     2701 ggcgacacgg tccaaaagaa ctggtttgag tcgggcacac ccgaaggtcg attcacaatc
     2761 cagagtctga gctaagcagg gttgacccgg cccgagacgt ggtcgtagaa cgggtccccc
     2821 ggcccgatcg aacctcgctc aaccggcgcc cccgtcatgc cggacttgta gagcgcgctc
     2881 aggaattcga tcgtcgggcg aatgtcgtct gttccggcag gcgggcgtcg ccccgcctca
     2941 agcacatcaa gcatcattgc aagctgtgcg ccgtgattag attccaggtc cgtttccggg
     3001 ggccatgtgc cagctggtag ctccgtgccc gctaccggcg tgatagtcca gttagcattt
     3061 cgatgacggt agagggcaac cacctccgcc gttgccctct ggaagtcgag ccgcagccgc
     3121 gtctcctgcc gcggcgacac gacgctactg attaccgacg ccaccgaacg gtcctcgaac
     3181 cgtaccattg caagggaagc atcctcgacc tcgatgtcgt gatccagcgt ctccgtaatc
     3241 gcggtgacat cacgccacgg cgtgttcagc caaagcatga ggtcgatcag gtggatcgcc
     3301 tgccccatgc tgacacctcc gagttcggat gcccatgtcc cgcgccacgg cacagcgtag
     3361 taggcatcgt cccgatacca ggtggtagcg cagatggaga cagtgtgtcg tccgaaggtg
     3421 ccggaatcga tcaagcgtcg gatctgctgt ccagccgagc cgaagcgcca ttgcacgacg
     3481 ctacagcaaa agcggccgct gcgctgctcc gcctctgcaa tgagatcaag ctgccgcaac
     3541 gatcccacga gcggcttctc gcagagcacg tgcgcacccg cctcgagaca ttgcagaatc
     3601 tgctgggcgt gcagggacgg tggtgtggcg atgtgtacta gatccggttg ctgctcgcgg
     3661 agcatctcgc cgacatcggc gtagctatgc ggaatgccgt gcttgttggc aaaggccgaa
     3721 actcgctcct cgtcaatatc gaccgctgcc acccgattca agcggtcctt acagtcatcc
     3781 caggcattca tgtgcgaacc ggcaacgcca cccgtcccaa tgatcgccac acgcagccgt
     3841 tccatcactt ccctctcctt caacatcgtc ccaaacccgc cgaacgatcc cggccgtcaa
     3901 tgtgtccatc cttcaggcag gtaggtgtcc agttgtccgc acatgcccac cgctggctcg
     3961 gccttcggca tgtaggaggc aacacgtgcc tgcatgagcg ctgtaatcgc ccctacctca
     4021 atctcgtcca gccgctcgcc ggtgcgttgc gcctccgcga gtggccgctc ctgccacagt
     4081 tgccgccact cggacaccgg atcccgccga agtgagacgg cattcttcag gaaccgctca
     4141 taccgggcca accggtcggt atcatcagcg tcgagcattg cagtgaatcc ctcgacagcc
     4201 agattgcgca gaaccgccgc gtcgacgggc cacttcactg cggacgcgac ctctgggccc
     4261 gccgccgacc ggtaagtatc agggtcggta aggataactt cgggaaccgt tagcacggca
     4321 tcacccgcct ctggtaatcc gttattctgc atgacgacga agatttccag atcgctgctg
     4381 gtgttgacaa gccgatggat gatgccgggc tcgaaccaga cgaccgcgcc gggccgcagg
     4441 tccaacgtcc cgaacccgtc aggacccagc gtatgcacct caccgtgccc ggcgaccgtg
     4501 acgtacgcct tcgtgctggc gaggtggaca tgcgggctgc cgccgcgtac gccatccggt
     4561 gcgacgctgt catagacccg caaccaggta aacccgacgg cgcttgggaa ctggggcagg
     4621 ccgaggtgct gccaagccat tgctacttat cctggagcca gcgctgatgt tcagcctgta
     4681 gtcgttcggc gtcccagatg ccatcgtgga tcaacacccg gtggcgaagc gccagcactt
     4741 cgcctttctg cagtttcatc ggcttgtgaa agagcagcgc ggcgttgagg aagttgcccg
     4801 accctgcccc accgccgtac cagggagccg ggtagcgggg gttatccggg tggtcaaaca
     4861 tcgccaaccc cgccgaaagg ttcgcaccgc cgtcgagcgg tccagacatc tcgcaccacg
     4921 cccccgtctg tccggccggg cggctgtcgg ctaccccgtc tggaagcagg aagcggtcta
     4981 ccagccagct acgcgacccg cggaacgaga gaccgccgta gccgccccaa gtggtatatg
     5041 gggtacggtc cagcgtgatg tcgcgttcgg cggtcaggac cgtcccccag tcgatgacga
     5101 aggcgtcatc agtggcacga gaccggatcg ttctgcgctc ggcgatcatt cgctggccgc
     5161 cgttcggcgc gatccagtcg agagcgaggc gcaactccac atcctccggt gaaccgtggg
     5221 taatctccgg gatgcccacg acgcactggg tgccgaaagg tggcctctcc tcccaaaagt
     5281 tgtcgccgtt cacgaatttg atggtgaacc agagccctcg gtgccagagg tggtcagccg
     5341 gctccagcag ggacaggaga tggccggcag gcgtctgcac tggatgcatg aatggcttgg
     5401 gcgcggtcgt gtaatccaat atccatgagg agtttcgctt gtcaatgggg aagcatgaga
     5461 tggtctttcc gacatcgtgc gagatgagca tgattcacct ttctcgtgcg aagcgtagcg
     5521 agaagtgggt acccagtcgt gcgatcaagt gagtagcacg atcctcgcgg cgtgatcttc
     5581 cggaatcgga ctggggaaga aaacgcctcg gcccagacgc gagaccatga gacgatattc
     5641 gtggaggagc cgggcggtct tcacaagccg aatgccttta atcttgttac accacagggt
     5701 aagctaggga atctcgggcc ggacgcagcc ctcaatcgcc tgtcggatta ttcaattgct
     5761 tcaccatctt ctgattcgga ctaagccggg agaaatcacc atgagcagag gaactcattg
     5821 ggccacgcct gccacctcga gaaaagagcg catccgccgt gttgaccgct ccggatccct
     5881 gaacaaccgc gccgcatccg ccagccggaa caattcagca agatgcacga cggagcgaca
     5941 actctcctgg gcgttcacca tgcggaaatg atcctgatgc ccattcaacc atgctaggaa
     6001 gaccacacct gtcttataga accgtgtggg cgccttgaag atatggcgcg acaacggcac
     6061 ggttggcgca aacaattcat catatcgatc catatctccg gcatccagag ctgcaaaggc
     6121 agctcccgcg atcggcgcaa tcggatcgaa gatgcccaag agggcgttcg agtagccctg
     6181 ctcgtcaccc ttgatgagat cgggatagtt gaaatcgtcg ccagtgtaca tccggacact
     6241 ttcgggaaga cggcggcgca tatcgacctc ccgctgatcg tcgagcaatg aaaccttgac
     6301 gccgtcaacc ttgtcagcgt gctgggtaat gatcgcaacg gcggtatcca ttgcctggtc
     6361 caggttgtcg ttaccccagt agccagcgag tgcagggtcg aacatgtctc ccaaccagtg
     6421 aatgatcacc ggctcactgg cctgctcgag gatacgcccg tagaccgacg cgtagtcatc
     6481 cgggctccgg gcacaggctg ccagtgccct gctcgccatc atgataatgc ggccgccgag
     6541 cccctcaaca taggcgaatt gctcctcgta agcagcagtc acatcatcaa gcgtaagttc
     6601 tggtcccagc ctcaactggt cggtaccggc accggaagcc agttgcccgc cgacagcttt
     6661 gccttccgtc actgagcgtc ggatgagctc cctggtcgtc tcccaatcga gccccatgcc
     6721 ccgctgcgcg gtatccatcg cctctgccac cctgagcccc atcgaccaga gatagcgacg
     6781 gtatgcgagg gtcgcctccc aatcgagcgc tacatcagtc gtcgggttga tgtcagccaa
     6841 agggtcgggt accacgtggg cggcagcaaa cgcgactcgt gtcctcggtt cgacaaagcc
     6901 ggtgccgtaa tccgcggcgg gacgcgccga gaaggcttca acttccccag agacagtcgg
     6961 caggttcagc gaggtcgacg ctagcgccat attcttatcc tttcagatgg ccaacaggat
     7021 ctggcgcgtc cagccgtcac cgttccttcc cccattgaag tctcaacccc gcaagccagc
     7081 tgaacgccct tgaccccttc aaacagatga tatggttgga gatcagcgac gacaatgtgg
     7141 cgcagaaact tttcccactg agccatgaag agttcttacg gtagaaagtc actgccaacg
     7201 aggagttgag gttcacaacg atgtcgtgat caatatcgaa ccgggaacgc gccgcgccac
     7261 cggctgcggc cgaatacggt tcgccctgtt catcaatgca tctcgagatt tgagtcgcgc
     7321 cgagcatctg tacagattcc gctgggccaa tcacattatc gagcatgtag cgccagtcgc
     7381 aaagtatgcg gaatggccgg ggctctaaca gcgccaatgc tgtttacgat agagcgacaa
     7441 ggaggggcgc aggacacttt cccgttgaaa catctttttc acgatttcct atatcgcaca
     7501 gaacgactcc tcatagcgct tcggaggttc ctaatgctaa cgtagatgca cacagtgact
     7561 tcatctcatc tcacaagggg agggataata gcatgaagtt tggagttttt cttcccacat
     7621 ttctgttccc aggcacagat gccggacacg ccgaacggct caggaggttc gcccgcaagg
     7681 cggaggaact tggcttcgac tcgctctgga tcaccgacca tatcgtcacc gcgcgcggcc
     7741 tttacgcggt cagctggctc gactccctga caaccctcgc ccatgtctcc gccatcacgg
     7801 agcgtgcgcg actaggaacc tctgtcctga tcctgcccgt gcgccagccg gtcgtcctgg
     7861 ccaaggagat cgccaccctg caacacctgt cgggcgggcg gtacaacttc ggcgttggta
     7921 caggctggta tggccccgag ttcgaatcgg ccggggtgca caagtcggaa cggggcgtcc
     7981 ggaccgatga agtgctcgat gccgtcaacc agcttctgcg agaaccaaac gtgaccttct
     8041 ccggtcgcta ctaccacctg aacgacgtga cggtggagcc cctctcgacc gtaccgcccg
     8101 tttgggtcgc cggcggtcgt caacttgtcc acgctgcctc tccggaaaaa tccgcgatgc
     8161 acccccgggt actcaagcgg atcgcccggt ccgacgcctg gatcgcgcgg ccgacctgcc
     8221 cgcccgactt gatcgcggtg gatatggccg agatccgcac cgcccgccaa gaggccggac
     8281 tggacggcga gccctttacc atggcgcacg agaacttcgt atacatcgtc gaagagggca
     8341 gcccagagga catcgtccgt gagcagcagg agaagtactt ccgcctgacc gggagtgccc
     8401 gcccatggga ctacattgag gccgtctacc tcaatggcac ggttgaccgc atccagtcaa
     8461 tggtcgatgc acggcggagc gtgggtatcg agtatatgat gctccataca ctcaccgatg
     8521 acctttcgca actggagttg atcgcccgcc atatcgtgca gccattcgcc aacgcataag
     8581 ctgcaagttg gccagctagc gagggatccg atgacggcct gtcccgaact gctgctctcc
     8641 gtggtgacac tggcagtgct caatgccgga gacgtcggac gacacgacgc catcctagct
     8701 cccgctcaat ccaagccatt ccttgacagt ctctgatagc gtcgttatga ttcgcccaca
     8761 gccacaatca actaagccag caaggaggga tactcagtgc ggaagagcaa actcagcttt
     8821 ggcatcctgg gctgcggtgt gatcggacca catcatgcag cggccatcac tgccctgagc
     8881 gatgaggcac acctcgtagc ggtggcggac tccgagccgg aacgggcgca gcagcttgca
     8941 caacagtatg gcgttcccgc ctaccaatcg atgcaggatc tggtagcacg ggacgacatt
     9001 gatgtgatca gcatctgcac tcctagcggc atgcacgccg agcatgcggt tgcggcaatg
     9061 gaggcgggga aacatgtggt cgtcgagaaa ccggtcgata tcaccctccc agcggttgac
     9121 aagctgatag cagtacagcg cgagaccggg cgaaaggcta cggtcatcag ccagcatcga
     9181 ttcgatcccg caagccaggt tgttcacgag gcagcccacg atggtcggtt cggacgcctg
     9241 acgatgggga acgcgttggt ccgctggtgg cgttcgcaag cgtattacga ctctggggcg
     9301 tggcgtggta cgtgggcgat ggatggcggc ggcgtgctga tgaaccagtc gatccacagc
     9361 gttgacctgt tgctgtggat gatgggtcca gtcgcggaag tgagcgcata caccggtctc
     9421 ctggcccacg aacgcctgga ggtcgaagac acagcggtgg ccgtcctcca gttcgcaagt
     9481 ggggcccttg ggatcatcga agggaccacc gccgcctatc cgggactcag tgcgcgtctg
     9541 gaaatctccg gtgaccgtgg gtccgcgact attgacgacg accgtctcgc gtactttcac
     9601 agtgccgccg atggcgagca ggccgacagc tatggaagcc gcggtgaagg caaccaagca
     9661 gacctggtgc tcccaagcga ggaggcgcac acgtctgccg gttcgaatcc cggcaatctg
     9721 tcgacgcgcc accgcgagca gatcagcgat tttatcgaag caattcacca ggagcgcgag
     9781 ccgcttgtca ccttggagga agggagaaag gccgtcgcag tcatcctcgc catctacgaa
     9841 tccgcccgga ccggacggcc cacacctgta gagggtgccc gcgtctagac ggatgcgtta
     9901 tcccattcat gcgatgccgc tccgtcgcga gcgaacaacc actgcagccc cggcggtacc
     9961 gaatacgtga aggggtgcca aacgcgaaag gaggtggcat gtgagttcga ccattgtcca
    10021 tcggggcaat ggagcgaccc ctcggagcat cattgcctga ggtcagcctg aagaggagtg
    10081 atttatgtcc agcaacgatc agacaaacgt ccgggcgttc gaccgccgca cactggttaa
    10141 gggcgtggct ggcacggcca tcggcgccag tgccttgccg accgccatgg cccacgcctc
    10201 aaccacgacg cgcggctcct ccatgaatcg gattcaagac ggtgagccca tcacgctcgt
    10261 ctatttcaac gcgcgggggg gggaagccgt cgaacgggcc ctggtcgagc gctacatgga
    10321 agagaacccc aacatcacga tcgagtacct gtcgactacc gcgattggcg ggccaagcga
    10381 cacggacacc atcgccaatc tcatcttcaa tatccaggcc gacaccaccg tcgatgtcgc
    10441 aaaagtggaa gtgcagcgga cgccccttga actgatggca acaggctccg cgctggaact
    10501 caccgagata gacgccgagg ccgtccgggc ccgatcagac gaactgttga ac
//
LOCUS       DASYWM010000199         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_383759, whole genome shotgun sequence.
ACCESSION   DASYWM010000199 DASYWM010000000
VERSION     DASYWM010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383759"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VGR29_08140"
     CDS             complement(<1..258)
                     /locus_tag="VGR29_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073594.1"
                     /translation="MNRAVAAIWSGMLIATTVGILPIVVRLLSRTLSAATNIERYTSE
                     ILAGGIGIAENTAKVAALKETIAIAPQLVAGAQSIERHAAAI"
     gene            complement(255..548)
                     /locus_tag="VGR29_08145"
     CDS             complement(255..548)
                     /locus_tag="VGR29_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073595.1"
                     /translation="MIVRFTILSAVEVLLFVGILIRFLGRIANTLERIGGSPTSSLAK
                     VSFGVRAIEKETSHLAPQVVALNDGLSALAAKLGVVDGHLEAVAERLTREEAS"
     gene            complement(568..882)
                     /locus_tag="VGR29_08150"
     CDS             complement(568..882)
                     /locus_tag="VGR29_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073596.1"
                     /translation="MPSPRDRSIPLHVIIRYWRVSLGMGLVVIGAVALLLDTLAKTTT
                     RIEAGAGEIWRVGKLIANNTVHVPLLVRTNQITADILPVVDGIASETMRIGRAVVGDA
                     NA"
     gene            complement(910..1221)
                     /locus_tag="VGR29_08155"
     CDS             complement(910..1221)
                     /locus_tag="VGR29_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073597.1"
                     /translation="MSEKQFVRYWRTGVSITVAVVATVAALLLAIIGTARSIHTHAQR
                     ALDLANEIVVNTRPIWDLEQTNVVATELREGALLIQRHAADVADALETPPRVTSDTVT
                     T"
     gene            complement(1248..2570)
                     /locus_tag="VGR29_08160"
     CDS             complement(1248..2570)
                     /locus_tag="VGR29_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HEV2073598.1"
                     /translation="MEEIALPVSPEERESVADRTKAPADWLTSSVEREMQRTPGSERV
                     GALLGPIKVVHAYWLAGMSCDGCTVAVTGATAPSVEDLLIGRLPGVPRVVLHHPVTSV
                     ESGEAFMRNYELAAEGKLDAPYVVIYEGSIADESLAAETGGYWCAMGSETVPKPIPTT
                     TWLKRLAPGAAAVIAIGTCATWGGIPAAYGNPTNSMSLMDFLGKDYLSALGLPVINVP
                     GCAPIGDNFTETIAAVLLFLNGLGPLPEFDELGRPAWLFNQTVHQRCVRAGFYEEGTF
                     AEHYGDPECLVEIGCWGPVVQCNITSRGAINHMGGCMNTGGVCIGCTMPGFPDRFAPF
                     YKRPPGTIISSTTARSVGSFVRPLRRMTMQFQNREPRWDKDGHVPSGWGNVPEPGPIK
                     KGVHYFYEKMQYFNAESPGRTKKVEQYPDGYEVAGVRSGETRPPEHLD"
     gene            complement(2620..3423)
                     /locus_tag="VGR29_08165"
     CDS             complement(2620..3423)
                     /locus_tag="VGR29_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2073599.1"
                     /translation="MKVAIAGKGGSGKTTIAGTLARVFAQDDHRVLAIDSDPNPNLAV
                     SLGIDAETAAHIDPVPTSLSHHSEDAEGCYSVGLSMSPEEISARYGSQAPDGVTLLLV
                     GRVEPHEAGVGCNCGAHRTVRGILEHLHQGDDFFIVTDMEAGLEHLKRGTLQHAKVLL
                     IIVEPYFKSLEAAGRIAELGRNLGVPALYAVANKVQSQRDEAAIRDFCRSHDLPVLAV
                     VPLDPDVSEAERQGKAILDVAPGAKAVLAVRELARDLLEEAAGRVNAAD"
     gene            complement(3420..3911)
                     /locus_tag="VGR29_08170"
     CDS             complement(3420..3911)
                     /locus_tag="VGR29_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV2073600.1"
                     /translation="MPNATSTVAQLAQLFGLSQPTVLEQVRRAVRDGLVVEVHLPESE
                     KRSAAERYYAPAVPVIRQPDHDLIESACHALAKDLSVALTENWADLQAAFAVTHLARE
                     GWTFDDLWPYLQETIVRLAMERIGGNPHAVPLRTHGLAWIQGLWNFEEMPVATVAMEE
                     GIA"
BASE COUNT          839 a         1307 c         1208 g          843 t
ORIGIN      
        1 gatggcggct gcatgccgct caatggactg ggctccagcc acgagctgtg gggcgatagc
       61 gatcgtctcc ttgagcgcgg cgactttcgc cgtattctcc gcgatcccga ttccaccggc
      121 gaggatttcg ctcgtgtacc gctcgatatt ggtggctgcc gacagcgttc gtgacaagag
      181 gcgaacgaca atcggcaaga ttccgacggt ggtggcgatc agcatgcctg accaaatagc
      241 agcaacggca cggttcatga tgcttcctcc ctcgtcaatc gctccgcgac tgcctctaag
      301 tgcccgtcga cgaccccgag cttggcagca agtgctgaga gtccatcatt gagcgcaacc
      361 acctgcggcg cgaggtggct tgtctccttc tcgatggcac gtacgccaaa gctcaccttg
      421 gcgagtgagc tggtgggaga gccgccaata cgctccagcg tgttagctat tcgccccaga
      481 aagcgaatca ggatccctac gaagagaagc acctcaacgg ccgagaggat agtgaaccgg
      541 acgatcatcg gtactccttc atggtgttca ggcgttcgca tcgccaacga ctgcacgacc
      601 gatacgcatg gtctcgcttg cgatgccatc aacaaccggg aggatatctg cggtgatctg
      661 gttggttcgc acgagcaatg ggacatgaac ggtattgttg gcgatcaact tgccgacgcg
      721 ccagatctcg cccgctcccg cttcaatccg agtcgtggtt ttggccagcg tatcgaggag
      781 caacgccacc gccccaatga cgaccagtcc catacccagg ctgacccgcc agtaacggat
      841 aatgacatgg agggggatgg accggtcgcg tggcgaaggc atggatctgt tctcccattg
      901 atggtcgctt caggtcgtca ccgtgtctga agttacgcgt ggtggtgttt cgagcgcatc
      961 ggccacgtct gcagcatgac gctggatgag cagcgctcct tcccggagct cggtcgcgac
     1021 cacgttcgtc tgctcgagat cccagatcgg tcgcgtattg acgacgatct cgttcgcgag
     1081 atccaatgca cgctgcgcgt gagtgtgaat actgcgcgcc gtgccaatga tcgcgagcag
     1141 aagtgccgca acagtggcga ccaccgcaac cgtgatcgag acaccggtgc gccaataccg
     1201 gacaaactgt ttctcgctca ttttcacctc acaatgatca atcgcatcta gtcgaggtgc
     1261 tcgggtggcc tggtctcgcc ggatcgaacc ccggctactt catacccgtc cgggtactgc
     1321 tcgaccttct tcgtccgacc aggtgattcc gcattgaagt actgcatctt ttcgtaaaag
     1381 tagtgaacgc ccttcttgat cgggcctggt tccggcacgt tgccccaccc acttggcaca
     1441 tgcccatcct tgtcccatcg tggctccctg ttctggaact gcatggtcat ccggcggagc
     1501 ggacggacga aggagcccac cgaccgcgca gtcgtcgagg agataatggt tcccgggggc
     1561 cgcttgtaaa atggagcgaa gcggtcaggg aaccccggca tcgtacaacc aatgcagacg
     1621 ccacccgtgt tcatacagcc ccccatatga ttgatggcac cccgggacgt gatattgcat
     1681 tggaccactg gcccccagca cccaatttcg accaggcatt cgggatcacc gtaatgctcg
     1741 gcaaatgtcc cttcctcgta gaagccggct cgaacgcagc gctggtgaac ggtctggttg
     1801 aacagccagg cgggccggcc cagctcgtcg aactccggca gtggccccag cccgttgaga
     1861 aagagcagca cagcggcaat tgtctcggtg aagttatccc caattggtgc gcagcccggg
     1921 acgtttatga cgggaagacc aagcgcgctc agataatcct tacccaggaa gtccattaaa
     1981 ctcatcgagt tcgtcggatt gccgtacgcg gctgggatgc caccccaggt agcacacgtg
     2041 ccgatcgcga tgacggcggc ggcaccaggt gcaagacgtt tgagccaggt ggtggtcgga
     2101 atcggttttg gcaccgtctc ggagcccatc gcgcaccagt aaccacccgt ctccgccgca
     2161 agagactcat cggcgatcga accctcgtag atcacgacat aaggggcgtc gagttttcct
     2221 tcggccgcga gctcgtagtt ccgcatgaac gcctcacccg actcgactga ggtgaccggg
     2281 tggtgcagga ccactctggg cacgccaggc agacggccga tcagcaggtc ttcgacactg
     2341 ggcgccgttg caccggtgac cgccaccgta cacccgtcgc agctcatgcc ggcgagccaa
     2401 tacgcatgca ctaccttgat tgggcccagg agggcaccca cgcgctcgga gccgggcgta
     2461 cgctgcatct cccgctcgac ggaagaagtc aaccaatccg cgggcgcctt ggtacgatca
     2521 gcgacggatt cgcgctcttc aggtgatact ggtagtgcaa tttcttccat cgcctccgct
     2581 cctcccgcca tacaaccgct tcatccactc ggatgctgtt caatcggcgg cattgactcg
     2641 gccggcggcc tcctcgagga gatcgcgtgc gagctcccgc accgctagaa ccgctttcgc
     2701 tccaggcgca acatcgagga tggcctttcc ttgccgttcc gcttcgctca cgtcggggtc
     2761 gagcggcacc acggccagga ccgggagatc gtgcgaccta cagaagtcgc gaatggccgc
     2821 ctcgtcccgc tgtgactgga ctttgttggc gacagcataa agggcgggca cgccaagatt
     2881 ccgtccgagc tcggcgattc gtcccgctgc ttcgagtgat ttgaagtagg gctccacgat
     2941 gatgagcagc accttggcgt gctggagcgt ccctcgcttg aggtgctcga ggccagcctc
     3001 catatcggtc acaatgaaaa agtcatcacc ctggtgaaga tgttcgagaa taccgcggac
     3061 cgttcgatgg gctccgcaat tgcagccaac gcccgcctca tgcggttcaa cccggcctac
     3121 gagcaagagc gttacaccgt ccggggcctg gctaccgtaa cgggccgaga tctcttcggg
     3181 agacatgctt aacccgacgg agtaacagcc ttcggcatcc tcagaatggt ggctgagcga
     3241 ggtcggcacc ggatcaatat gcgctgccgt ttccgcatca atgcccaggc tcacggccag
     3301 gtttgggttg gggtcggagt ctatggccag aacccggtgg tcatcttggg caaacacgcg
     3361 tgcaagcgta cccgctatcg tcgtcttgcc tgacccgcct tttccagcta tcgcgacttt
     3421 catgctattc cctcctccat cgctaccgtg gcgaccggca tctcttcaaa attccacaag
     3481 ccctggatcc aggcgaggcc gtgcgttcgg agcggaacgg catgcggatt accgcctatc
     3541 ctctccatgg cgagacgcac gatcgtctcc tgaaggtatg gccagagatc atcgaatgtc
     3601 caaccctccc gagcaagatg ggtaacggca aaggcagcct gaaggtctgc ccagttttcc
     3661 gtcaaagcga ctgaaaggtc cttcgcaaga gcgtgacagg cactctcaat cagatcatga
     3721 tcgggctgac gaatcactgg cacggctggc gcatagtagc gctctgcagc ggaccgcttc
     3781 tccgattcag ggagatggac ctcgacaacc agtccgtcac gtaccgctcg acgaacttgt
     3841 tccaataccg ttggttgcga taaaccgaac aattgtgcaa gttgcgctac cgtgctggtg
     3901 gcgttcggca agggcggcga ctagcctgtc gcgagctgcc agcggcataa ccggtggcga
     3961 agtctccccg ggcacctgga cctgtttgac ccaatgcaac tgtttcggcc gtacagaagc
     4021 catatattgt tccttaggca agtccctaag cgtgcgataa attgcttata gcatccggga
     4081 cgcagtgcgt caagcctgtc aaggaatgcg cagctgtctt gcagatcaac ttctcactcc
     4141 ttggggaccg atctgcagtc gtgccggatg gtgatatacc gcgcttcctc tcccccc
//
LOCUS       DASYWM010000200         5879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_177265, whole genome shotgun sequence.
ACCESSION   DASYWM010000200 DASYWM010000000
VERSION     DASYWM010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177265"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /gene="rsmH"
                     /locus_tag="VGR29_08175"
     CDS             complement(<1..880)
                     /gene="rsmH"
                     /locus_tag="VGR29_08175"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV2073601.1"
                     /translation="MEPQSQVPALLEGHVTVLLKEAVRALKPRSGGTYIDGTFGGGGH
                     SSLLLQRLNGDATLYAIDADPDAVTRAQRLADSPLGSGVRPVHANFRDLQQIAQDHEI
                     AEVDGILLDLGLSSFQLDQAERGFAFRVDGPLDMRFDPAQGPSAADLVNTLDTDDLAR
                     LLWHYGEESNSRRIARAIVARRETEPIETTTDLGALVERAVGGRRGKAIHPATKTFQA
                     LRIAVNDELTALEHVLEVAVTRLKPGGRLVVIAFHSLEDRLVKQFITRESATCICPPE
                     QPVCTCDHTPRLRRIGK"
     gene            complement(977..2752)
                     /locus_tag="VGR29_08180"
     CDS             complement(977..2752)
                     /locus_tag="VGR29_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEV2073602.1"
                     /translation="MTQRTINRILVANRGEIALRIMRTCHEMGIGTVAVYGDGEEHAQ
                     HVLYADDAWRIPDGQGLPYLRINALLEIAHKSGAEAVHPGYGFLAENADFARAVQNAG
                     LIFIGPPADAIAAMGDKVEARRIARSAGVEPVPGTTEPVETIDDALSWTETHGYPVAV
                     KASGGGGGRGFRVARSADEMKDAFEGSRGEAERYFSNPEVYLERYIEQPRHIEVQVFA
                     GAQGEVLAFPERECSIQRRHQKLIEETPSPAVDVQLRERLERATISLVEAVDYLGVGT
                     VEYLLDADGSFYFLEMNTRIQVEHTVTEMVTGIDIVQAQIQAAMGLPLGFTRDEVEPS
                     GWSIECRINAEDAARQFAPVTGMLTKYLPPDGFGVRVDSALVQGDRILPAYDSMIAKL
                     IVWGRDRDEAIARMYRALKDYQIEGVPTTIPFHKRMMEHEAFGAGETTTTFLANHPEV
                     LEGYSPADMPAQEEQTPGSPALALVVEVGGRRFDVAVSGLPTNGGSRRQSGRTHRPGP
                     PKSGSGGAGSGNDLISPIQGTVLRVAVEKGQTVARGDLVCVVEAMKMENEMTAHRDGI
                     ISALNVVAGATVQISGIIATIEDLE"
     gene            3012..4742
                     /locus_tag="VGR29_08185"
     CDS             3012..4742
                     /locus_tag="VGR29_08185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2073603.1"
                     /translation="MSHTRIRGSKSNAAAPDAPSHFSHGPIVLVFAVFLLAMFVIPTF
                     NNVPVGDDWVYTRSVEMLVEDGRLEILDLSVVTLVFQVLWGALFSAFEVSFASTRLST
                     IALVLLSTAALYALCRDMRVSPQRAALGAAVYLFNPLTFGLAFTFMTDPHFTGLLVIS
                     TWLYIRGLRPEQYSPRTVLFASAIAALAFLVRQQGILIPASVGLYLLFASRWKPNAAG
                     ARMAGIVGGIPALAVVLYYAWLFLIHGAPEQQGAFLDEMIDAGAHESRILASRMTYIA
                     LAYMGVFVLPIALGAVPALRSLCMPQSWAGRLIAGIWLIALIAGAVIFTQAGRHMPYI
                     PQYASPQGIGPTDLYGGRPWLLGGDVVSWLSWIAVTSSAAFILLIATRLFDRATPERA
                     GASLLCMIGFWQFIGALPPSFHFRNWIISVDRYLLPLVPISVVLLLWALNDRRLILPI
                     AWVTTALIAAFSIIGTRDFLVFQGATWEMAHEAVESGVPLVQLDAGASWDGYYLYDYS
                     IAHGIDQRTPGGPWWTDLFGPATDSTYVVSTRVLPEHTVVKQDAFSSWMDGDTRTMYL
                     LRKADSPRTP"
     gene            4806..5558
                     /locus_tag="VGR29_08190"
     CDS             4806..5558
                     /locus_tag="VGR29_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2073604.1"
                     /translation="MWNGRTVSVVLGTYAEKDSIQGVINGFFATGVVDEVVVVNNNAE
                     PGTREEVEQTEARQVFESKQGYGYSYRRGLAEATGDLLILAEPDGTFLPRDAVKLLTY
                     SDDCDAVFGTRTTREMIWSGANMGTFLKWGNWAVAKLVEVLFNTSHLSDVGCTYRLLT
                     RDTLEKIQPKFTVGGSHFGPELMLLVITSGARVVEVPVNYLPRVGESSVTGDLRKAVK
                     LGFQMIGFILKFRLRVARGGRVKAAPRMLDGD"
     gene            5562..>5879
                     /locus_tag="VGR29_08195"
     CDS             5562..>5879
                     /locus_tag="VGR29_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2073605.1"
                     /translation="MKRTLSPVAPTALRPKTTGENGQTDFDAVADEYDESLPVHVMEH
                     YIDKRTAFVREHVASGSKILDVGCGTGVLAERLLQQGYDVTGADPFAAMLHHMAKRDS
                     RLKT"
BASE COUNT         1127 a         1804 c         1678 g         1270 t
ORIGIN      
        1 gcttgccaat ccgtcgtagg cgaggagtat gatcgcacgt gcagaccggc tgctccgggg
       61 gacatatgca ggtggcagat tcgcgcgtga tgaactgctt gacgagccga tcttcaagcg
      121 agtggaaggc gatcacgacc agccgtcctc ccggcttcaa gcgtgtcact gcaacttcaa
      181 gcacgtgctc cagtgccgtc aactcatcat tgacagcgat gcggagcgcc tggaaggtct
      241 tcgtcgccgg atggatcgcc ttgccacggc gaccaccgac agctcgctcc accaacgccc
      301 cgagatccgt ggtggtctcg atcggctccg tctcccgcct ggcgacaatc gccctagcga
      361 tacgacggga gttcgattcc tcgccatagt gccaaagcaa gcgagccaga tcatctgtgt
      421 caagtgtatt cacgagatcc gcggcagagg gaccctgggc cgggtcgaac cgcatgtcga
      481 gcggtccatc gacgcgaaag gcgaatcccc gttcggcctg gtctagttgg aacgacgaca
      541 accctagatc aagcagaatg ccgtctacct cggcaatctc atggtcctgc gctatctgtt
      601 gcagatcgcg gaagttggca tgcaccggcc tgacaccgga acccagtggc gagtcggcca
      661 agcgttgggc tctggtgacg gcatccggat cggcgtcgat agcgtacagc gtcgcgtccc
      721 cgttcaggcg ctgaagcagc agtgatgaat gcccgccgcc gccaaacgtg ccatcgatat
      781 acgtgccgcc ggacctgggt ttcaaggctc tgaccgcttc cttcaggagg accgtgacat
      841 gtccctccag gagcgctggt acctgacttt gtggttccat aagtaactcc ggaacatgtc
      901 atgggctatg tcgcctaacc atggggatgc taccgcattc cgatgtatgg acgaggcgac
      961 cgtcggctca gcctggttat tccaaatcct cgatcgtcgc gattatcccg ctgatttgga
     1021 cagtcgcccc ggccaccacg ttgagtgcgg agatgatccc atcccgatgc gcggtcatct
     1081 cgttctccat cttcatcgcc tcaacgacgc agacaagatc accgcgagcg acagtctggc
     1141 ccttctccac ggccaccctc aacaccgtcc cctgaatcgg gctgatgaga tcattgccac
     1201 ttccggcacc tccacttccc gatttgggag gacccggtct atgcgtccga cccgactgcc
     1261 ttcttgaccc accgttggtc ggcaggccgg agacggcaac atcgaaccgc cgaccgccga
     1321 cctcgacgac aagcgccaac gcgggagagc ctggtgtttg ctcttcctgg gctggcatat
     1381 cggcaggcga gtagccctcc agcacttcag gatgattcgc aagaaaggtg gtggtggttt
     1441 ccccggcgcc aaaggcctcg tgctccatca ttcgcttgtg gaacgggatt gtcgtcggta
     1501 cgccttcgat ctggtagtcc ttcaatgccc ggtacatgcg ggctatcgcc tcgtcgcgat
     1561 cgcggcccca cacgataagc tttgcgatca tcgagtcgta cgccgggagg atccggtcgc
     1621 cttgcacaag ggcactgtcg acacggacgc cgaagccgtc gggagggagg tattttgtga
     1681 gcatgcccgt cacgggtgcg aattgcctcg ccgcatcctc ggcgttgata cggcactcga
     1741 tcgaccaacc actaggttca acctcatccc tggtaaagcc gagcggcaaa cccattgcag
     1801 cctggatctg cgcctgaaca atatcaatgc ccgtcaccat ctccgtcact gtgtgctcga
     1861 cctgaatcct cgtgttcatt tcgaggaagt aaaaggaacc atcggcatcc agcaaatact
     1921 caacggtgcc gacaccgaga tagtccacag cctcgaccag tgagatcgtc gcccgctcaa
     1981 ggcgttctcg tagctgcacg tctacggcgg gcgacggtgt ttcctcgatc agtttctgat
     2041 gccgccgctg aattgagcat tcgcgctccg gaaaggccag cacctcgccc tgcgcacccg
     2101 cgaatacctg tacctcgata tggcgcggct gctcaatata gcgctcgaga tagacctcag
     2161 ggttggagaa ataccgctct gcctcgcctc ggctcccctc gaacgcatcc ttcatttcgt
     2221 cggcgctgcg cgccacacgg aatccgcgtc ccccgcctcc gcccgacgct ttcaccgcca
     2281 ccggatagcc gtgcgtttca gtccatgaaa gggcatcatc aattgtctcg acgggttcag
     2341 ttgtgccagg tactggttcg acaccggccg agcgcgcgat tcggcgggct tcgaccttgt
     2401 cgcccatagc agcgatggca tctgcaggag ggccaataaa gatcaaaccc gcgttctgga
     2461 ccgcccgcgc gaaatcggca ttctccgcca ggaacccata tcccggatgc accgcctcag
     2521 cgccggactt gtgtgcgatc tccaggagtg cgttgattcg cagataggga agtccctgac
     2581 cgtcggggat tcgccaggcg tcgtcggcat agagcacgtg ctgcgcgtgt tcttcaccat
     2641 cgccatacac ggcaacagtg ccgatgccca tctcgtggca tgtgcgcatg atgcgcaacg
     2701 cgatctctcc ccgattcgca accagaatcc gattgatcgt gcgctgggtc attccacaat
     2761 tctctctcgc aggaagggtg cgacgcatca ccaccagaag gccaccggac tgataaatcc
     2821 acgcccggaa agatagcatg gtgacgctcc gtatccggtg cctatctcga ttaggtggta
     2881 agttgcttgt gcccggccca agtattgcgc agggcagcct gattgtcggc aacggtgtat
     2941 aggcctgatc cgcatagtga ttccgcatag tgatgaaggc cacctcgaag atcagagggc
     3001 aaccgggaca catgagccac acacgtatac gaggatcaaa gagcaacgca gccgctcccg
     3061 atgcgccctc tcatttttca cacggcccga ttgtcctggt attcgccgtc ttcctcctgg
     3121 cgatgttcgt catccccacg ttcaacaatg tgcctgtggg ggatgactgg gtttacacac
     3181 gttcagtcga gatgctagtg gaagatggac gactcgagat ccttgatctc tcggtcgtca
     3241 ctctcgtctt ccaggtactg tggggcgcac tcttttccgc tttcgaggtg tcgttcgctt
     3301 ctaccaggct ctcgacgatt gccctcgttc tgttaagcac cgccgcactg tacgcgctct
     3361 gcagggatat gcgggtctct ccgcaacgcg ccgcactcgg tgcggcggtc tacctcttta
     3421 accccctgac cttcggcctc gccttcacct tcatgactga cccacacttc accgggctgt
     3481 tggtgatttc gacctggctc tacattcgtg gattacgccc cgagcaatac agcccgcgca
     3541 cggtgctgtt cgcgtccgcg attgcggctc tcgccttcct ggtcaggcaa cagggcatcc
     3601 tcatcccggc gtctgtgggt ctctacctcc tcttcgcatc ccggtggaaa ccaaatgctg
     3661 caggggcaag gatggcaggg atcgtcggag gtatacctgc gcttgcggtc gttctctact
     3721 atgcatggct cttcctcatc catggggcgc cggagcaaca aggcgcgttt ctggatgaga
     3781 tgattgatgc cggtgcccac gagtcgcgca tcctggcaag tcgaatgact tacattgcac
     3841 tcgcctacat gggtgtcttt gttctcccga ttgcacttgg ggcagtacca gcgctgcgct
     3901 ccctgtgcat gccgcagtca tgggccgggc gcctgatcgc gggaatctgg ttgattgcat
     3961 tgatcgccgg cgcagtcata ttcacccagg cgggtcggca catgccctat atcccgcaat
     4021 acgccagtcc tcaagggatc ggacctacgg atctctacgg cggacggccc tggctactag
     4081 gtggtgacgt ggtgtcatgg ctcagttgga ttgccgtaac ctcctcagca gcgttcattc
     4141 tgctcatcgc cacccgcctc ttcgatcgtg ctacacctga gcgcgccggc gcctcattgc
     4201 tctgcatgat cgggttctgg cagttcatag gtgccctgcc gccgtccttc catttccgga
     4261 attggatcat ctcggtggat cgctacttgt tgcccctggt gccaatcagt gtcgtcctcc
     4321 tgctttgggc gctgaatgac cggcgtctca tcttgccaat cgcctgggtt acgacggcgc
     4381 tcattgcggc attctcgatc atcggcacgc gggatttcct tgttttccag ggtgcaactt
     4441 gggaaatggc acacgaagcc gtggagtcgg gcgttcccct cgtgcagttg gacgctggtg
     4501 cgagttggga cggctattat ctctacgact actccatcgc gcacggtatc gaccagcgaa
     4561 caccgggagg tccctggtgg actgatctct ttggtccggc aacggactca acgtatgtcg
     4621 tttcgacacg tgtgcttcct gagcacacgg tcgtaaaaca agatgcgttt tcatcctgga
     4681 tggatgggga tacgcgaacc atgtatcttc tgcgaaaggc agattcgcca aggacaccgt
     4741 gaagcccacg ttgttcccag atcaccaagg gtcgccatca cggtcatcag aaggacacct
     4801 gcttcatgtg gaacgggcgc acggtatctg tcgtactcgg cacgtatgcc gagaaggact
     4861 ccattcaggg tgtgatcaac ggcttcttcg ccacaggcgt ggtcgatgag gtggtcgttg
     4921 tcaataataa tgctgagcca ggtaccaggg aagaagtcga gcagaccgaa gcgcgtcagg
     4981 ttttcgaatc gaagcagggt tacggctatt cctatcgacg gggccttgcg gaagcgacag
     5041 gagatctcct gatccttgcg gagccggacg gcaccttcct gccccgggat gccgtcaagc
     5101 tcttgaccta ctccgatgat tgcgacgccg tcttcggcac ccgaaccaca cgcgagatga
     5161 tctggagcgg cgccaacatg ggcacgttcc tcaaatgggg aaactgggct gttgccaagc
     5221 tggttgaagt cctcttcaac accagtcatt tgagcgatgt cggttgcacc taccggctcc
     5281 tgacacgcga cacgctggaa aagattcagc cgaagttcac cgtcgggggt agtcactttg
     5341 gccctgagct gatgttgctg gttatcacgt ctggcgcccg cgtcgtcgaa gtgcccgtca
     5401 actacctccc acgcgtcggt gagtcctccg tcactggcga tcttcgcaag gccgtcaagc
     5461 ttggatttca gatgataggc ttcatcctga agttccggct aagagtagcg cgcggcggac
     5521 gcgttaaggc cgcccctagg atgcttgatg gagactgatc agtgaagaga accttgagtc
     5581 cggtggcacc aaccgcactg aggccaaaga cgaccggtga aaatgggcag actgactttg
     5641 acgccgtcgc cgatgaatat gacgagagcc tcccggttca tgtcatggag cattacatcg
     5701 acaagcggac ggcgttcgtg cgtgagcacg tcgcgagtgg tagcaagatt ctggatgtcg
     5761 gctgcgggac cggggtcttg gcggagcggt tgctccaaca gggatatgac gttaccgggg
     5821 cggatccctt cgccgccatg ctgcatcata tggcgaagcg agattcgaga cttaagacg
//
LOCUS       DASYWM010000201         4215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_83414, whole genome shotgun sequence.
ACCESSION   DASYWM010000201 DASYWM010000000
VERSION     DASYWM010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83414"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VGR29_08200"
     CDS             complement(<1..725)
                     /locus_tag="VGR29_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV2073606.1"
                     /translation="MQPAPARPRGMVPIPGGTFRMGNPRGDGYETDGEGPVHEVTVDP
                     FWMDATAVTNARFAQFVTTTGYITEAEAFGWSFVFVGLLPPTLQNMQRPVVAPWWRQV
                     FGADWRHPEGPGSDSARRLDHPVVHVSWNDAHAFCTWSGTRLPTEAEWEFAARSGREG
                     ARFPWGNELTPTGKHRMNVWQGTFPGRNTRADGHLGTAPVTSYQANAYGLSCMTGNVW
                     EWCADWFSPETYAVSDGTNPTGP"
     gene            complement(939..1478)
                     /locus_tag="VGR29_08205"
     CDS             complement(939..1478)
                     /locus_tag="VGR29_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2073607.1"
                     /translation="MSIPILTTDRLELRPFSEDDIDRVTELLQAPEIAATTLNIAYPY
                     SRDDAAGWIARHPEAAEAGTDLTWAVWLQDDDLLIGAIGIHIDVHNRRGTLGYWLGRP
                     YWNKGYMSEAARAVVEYGFSKLDLHRIEATRMPENIGSGRVMEKAGLTYEGTLRGYYV
                     KDGVFLDAAIHAIIRNEAE"
     gene            complement(1648..1977)
                     /locus_tag="VGR29_08210"
     CDS             complement(1648..1977)
                     /locus_tag="VGR29_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073608.1"
                     /translation="MSDQLRLRAGIRLLADRKGVPISELEALPVEKIEKDIRAARVTT
                     WDIARQVAIGLNLQRLYVQFTIPETMGISEAVEHGLISRGDALACYAVLPEEIQAIIR
                     EHHWVAA"
     gene            complement(2355..3764)
                     /locus_tag="VGR29_08215"
     CDS             complement(2355..3764)
                     /locus_tag="VGR29_08215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073609.1"
                     /translation="MGYTATGMRTKTPKDPPDPDNTGNPDSQQGIPNPLRAVRSVLLQ
                     GDRNLGRDAVGRFFRSPKGKISWRRNLYAIWAAQLLAIMGFSLRTPFLPLFFGDLGVD
                     TRAGQALWTGIILSVGAAMMAITSPIWGAIADRKGRRPMLLRAQFASFVTIGLTAFVT
                     APWQILGLRVVEGALAGTVTAATALIAVTMPKERLGFGLGMIQTAVFSGSAIGPLAGG
                     LLADHLGYRPTFVVASLMLLSAGFITLLLVQERFEPAEQTENDPKEASAWRLLLGPVL
                     LSLTFAMLAIRFASSAVQPVIPLFVAQLSDGSGSTSSLAGITLGVLGLTSAVSSVYFG
                     RLGDKRGHHLILIGAALGAGLIYLPMSAVQNAWQLIVLQAVFGVFAGGMIPAANALIA
                     SVTDPSRRGTVFGLMNTAASLGGFLGPLAGAGLAASIGFRATFLATGLVLLGMVAMLY
                     VTGKKHPMDPAEGATPAHA"
     gene            3958..>4215
                     /locus_tag="VGR29_08220"
     CDS             3958..>4215
                     /locus_tag="VGR29_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073610.1"
                     /translation="MRVFRTGFALLALVLAMMNIGMERAVAVQATGTVEVHARSCDQA
                     YTGDAWFTGCHESTAPDTSFEATNTETGEVVSGTTDASGNLV"
BASE COUNT          789 a         1331 c         1344 g          751 t
ORIGIN      
        1 tccggcccgg tgggattcgt tccatcgctc actgcatagg tctccggtga aaaccagtcg
       61 gcgcaccatt cccagacatt gccggtcatg caagagaggc cataggcgtt cgcctggtag
      121 ctcgtcaccg gtgccgtgcc caggtgccca tccgcgcgag tgtttctccc cgggaaggtg
      181 ccctgccaga cgttcatccg gtgcttgccc gttggcgtta gttcattgcc ccacgggaag
      241 cgcgctccct cccgaccact tcgcgcggca aactcccatt ccgcttccgt cggcaaccgg
      301 gttccagacc aggtacagaa ggcgtgggca tcgttccaac tgacgtggac caccgggtga
      361 tccagccgcc tggcactatc gctgcccggt ccctccggat gccgccagtc ggcaccaaag
      421 acctgtcgcc accagggagc gacaacggga cgctgcatgt tctgcaacgt gggtggcaag
      481 aggccgacaa agacaaacga ccagccgaag gcttcggcct cggtgatgta gccggtagtc
      541 gtgacgaact gggcgaagcg cgcattggtc accgccgtgg cgtccatcca gaagggatcg
      601 actgtgacct cgtgcaccgg tccctccccg tccgtttcgt agccatcgcc acggggattg
      661 cccatgcgaa acgtcccacc ggggatcggc accatgcctc tgggccgggc gggcgcaggt
      721 tgcacctcag gcgtctcccg ggttgtggct ctcgctcctc gggacgggct gcagcagccg
      781 ctcatgcgaa gcgctccatg acgctaagta gcgcgggcac cagggaatgg caaggaatga
      841 gcatgatgtc atctccagcg acgcgtctca ccacactgtc caggtgttct gccactggtc
      901 gcatgcgacc acagggaggt tgaggcaccg atcacccctc attccgcctc attccggatg
      961 atggcgtgta tcgcggcgtc gaggaagacg ccatccttca cgtaatatcc ccgcaaggtg
     1021 ccctcgtagg tcaggccggc cttctccatg acgcggccgg agccgatgtt ttccggcatc
     1081 ctggtcgcct cgatgcgatg gagatccagc ttcgagaatc cgtactccac cacggcgcga
     1141 gcggcctcgc tcatgtagcc cttgttccaa tatggtctgc cgagccagta tccgagcgtg
     1201 ccccgccggt tatggacatc gatgtggatg ccgatcgcgc cgatcagcag gtcgtcatcc
     1261 tgtaaccaaa ctgcccaggt caggtccgtg cccgcttccg ctgcctcagg atgcctcgct
     1321 atccatccgg cggcatcgtc ccgggagtag gggtaggcga tgttgagtgt ggtcgccgcg
     1381 atctccggtg cctggagcag ctccgtcacg cggtcgatgt cgtcctcgct gaaggggcgc
     1441 agctccaggc ggtcggtggt caggatgggg atactcatcg agtgctggcc tccttcgcga
     1501 ttccttgtgt gcctgccatg ccattctatc cctcgtgtcc cacgatcgca gcaggagacc
     1561 ggggagatga cgcaaggaac cccggctggt atggaaccaa ccggggctct tcggggagga
     1621 ttgcggagtg ttggacgatg cctgggatca ggcggcgacc caatggtgct cgcggataat
     1681 ggcctgaatc tcttccggca acacggcgta gcaggcgagg gcatcccccc gcgagatcag
     1741 gccgtgctcg acggcttcgc taatccccat cgtttccggg atggtgaact ggacgtagag
     1801 ccgctgcagg ttcaggccga tggcgacctg gcgggcaatg tcccaggtcg tgacgcgcgc
     1861 cgcgcggata tccttctcaa tcttctctac cggcagagcc tcaagctcag agatcggcac
     1921 gcccttgcgg tcggcaagca atcgaattcc ggctcggagt cgcagctgat cggacatatc
     1981 agaatgtacc aagttaatct gccctttcta cgaaatatcg tgaaggaaga agcggtttct
     2041 acatgtccgg atcgtcgctc ccatcatcgt tatcacagcg gttgcggtcg atgcggtgac
     2101 aagtgcaaag atatcggcaa ctggcccatc ccaaggggaa ggaccggctt gggggccagg
     2161 ctcacacgag aaaccctatt caactatcgg tattttacca taaatagata ttgtatgcaa
     2221 ttcgcggatc gttaaatgaa tgtgcaggaa agcatcaccg aatgaaggcg cggaatccgc
     2281 actcctgacc cacccggccc gcatgccatc atgccagagg gcggttgggg tgaggtgggg
     2341 aaggcgtctt cctcctaggc atgggctggc gtggcgccct cggctgggtc catggggtgc
     2401 ttcttcccgg tgacgtaaag catggccacc atcccaagga gcacgagtcc ggtagcgagg
     2461 aatgtggccc gaaagccaat gctggcagcc agtccggcgc cggcgagcgg gccgaggaac
     2521 ccgcccagcg atgccgccgt attcatcagc ccgaagacgg tcccccgacg gctggggtcg
     2581 gtcacactcg cgattagtgc gttggcggcg gggatcatcc cgccggcgaa gacgccgaag
     2641 acggcctgca gcacgatcag ctgccacgcg ttctgcaccg ccgacatcgg caggtagatc
     2701 agcccggcac cgagagcggc cccgatcagg atgaggtgat gccctcgctt gtcacccagc
     2761 ctcccgaaat agacggacga gacagcgctg gtcagcccca gcacaccgag cgtgatgccg
     2821 gcgagggagg acgtgctgcc ggagccatcg gacaattgcg ccacgaagag ggggatgacc
     2881 ggctgcacgg cggacgacgc aaaccggatc gccagcatcg caaaggtgag cgagagcagc
     2941 actggaccca gcaacagtcg ccaggcggag gcctccttgg ggtcattctc cgtctgttcc
     3001 gccggctcga accgctcctg caccaggagg agggtgatga acccggcgct cagcaacatc
     3061 agcgaggcga caacaaacgt tggccggtag ccgaggtggt cggccagcag cccccccgcc
     3121 agtgggccga tcgcggagcc ggagaagacg gccgtctgga tcatgccgag cccgaagccg
     3181 agccgctcct tgggcatcgt cacggcgatc agcgctgtcg ccgcggtgac ggttcccgcc
     3241 agtgccccct ctaccacgcg taagccgagg atctgccatg gcgcggtgac gaatgcggtg
     3301 aggccgatag tgacgaagga agcgaactgg gcccgcagca gcattggcct gcggcccttg
     3361 cggtcggcga tcgcacccca gataggcgag gtaatggcca tcatggcggc gccgaccgag
     3421 aggatgatgc ccgtccagag cgcctggccg gcccgtgtgt cgacaccgag atcgccgaag
     3481 aagagcggca ggaagggggt acggaggctg aaccccatga tcgccagcag ctgcgcggcc
     3541 cagatcgcat agagattgcg tcgccacgag attttgccct tcggcgagcg gaagaagcgg
     3601 ccgacggcat cccgcccaag gttccggtct ccctgaagca gcacggaacg gacggccctg
     3661 agcgggttcg ggatgccctg ctggctgtcg ggattcccgg tattatcggg gtcgggtggg
     3721 tctttcggcg tcttcgtcct cataccggtt gcagtgtatc ccactggccc ccttgccgga
     3781 acaggtggcc cgctccatgc tgtataacgt gcgaccgccc gccggttgct gctctggtcg
     3841 tgcgattcgc tcggaggcac agaataccga gccgatatcg cctatgcagc cgtggcatgc
     3901 aggtctgccc caagagcggc acatcatatg tccgatttat attggaggag gtgtcgaatg
     3961 cgagtgttcc gaacaggttt cgccttgctg gccctggtgc tggcaatgat gaatattggt
     4021 atggaacgcg ccgtcgccgt ccaggctacc ggcacggtgg aggtacacgc gcgaagctgc
     4081 gaccaggcct acaccggtga cgcctggttt actggctgtc acgagagcac cgcgcccgat
     4141 acctctttcg aggccaccaa taccgagacc ggcgaagtcg tctccggcac caccgatgca
     4201 agcggcaatc tcgtc
//
LOCUS       DASYWM010000202         7570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_308481, whole genome shotgun sequence.
ACCESSION   DASYWM010000202 DASYWM010000000
VERSION     DASYWM010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308481"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VGR29_08225"
     CDS             <1..1261
                     /locus_tag="VGR29_08225"
                     /inference="COORDINATES: protein motif:HMM:NF027305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV2073611.1"
                     /translation="FLSMNSQERNGLIRLVMEGHLRGITGQLTVEAIVKEPEMVADRM
                     RANVAEDMAKMGLEVVSFTIREVKDSNEYILNMGKPDIANVRRVADIATAEADRDTAI
                     RRAQASREARIAEAAAEQEKVTAESMSQTKQAEAVRDLELKRAEFDRSVQEQRAQADK
                     AYEIQASVQQQRIMAEQVRIEQVRKEGEIAVQEAEIIRREKELIATVLRAAEVEKKRI
                     ETLAEADRQCQVLRAQAVAEQTRLEGEAQAEIVRIQGQAEADVIAAKGQSEAHAMQIK
                     AAAFQEYNQAAVLDKLISQMPEVVRAISEPLNKVDKITIVSTGGDGQNGTTGVNRLAG
                     DMTSLVAQVPALLESLTGVKLEDLLKHVPETGDTYHRSGENASPARKTDRRDSPMLVV
                     DAEPMKAENGKARRPSKDGQGSQESPE"
     gene            1341..1868
                     /locus_tag="VGR29_08230"
     CDS             1341..1868
                     /locus_tag="VGR29_08230"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEV2073612.1"
                     /translation="MEPSAPGGDAQLLQVEALGEPGHRRFRIVAVIESETYILWMEKQ
                     QVQALGLALEQLIEQVPGSPGAGGVGNAFAIVNEETRNQFRVGRIEIAFDDLIEQILI
                     SAHDIEQDTPDAPPTLAIRISREQARELSTEAADLVARGRPICPMCGSPMEREGHVCP
                     QQNGHLPLAFGDELT"
     gene            2471..4240
                     /gene="ilvB"
                     /locus_tag="VGR29_08235"
     CDS             2471..4240
                     /gene="ilvB"
                     /locus_tag="VGR29_08235"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEV2073613.1"
                     /translation="MMVAVESRSLTGEVPAQSPIQTRPEILSGAQIVCRSLEEEGVTT
                     VFGYPGGAVIPLYDALPPTNLHHVLTRFEQWAGMAAIGYARVTGEVGVCIATSGPGAT
                     NLVTALADAMLDSVPLVAITGQVMQGLIGRDGFQEVDITGITLPVTKHNYLIRRPEDI
                     ASTLKEAFYLARSGRPGPVLVDIPKDVFIAKAPYTYPETTGRKSYQPNLVPNMRQVRL
                     AAEMINSAKRPLIMAGHGIILSGAEAAFRDFTEKAGIPVIFTLLGLGALDEGHPLSLG
                     MMGMHGHRAANRALDECDLLVNFGARFDDRATGNLSGFAPNAKVIHVDIDPAEIGKNV
                     PTDVPVVGDAGEALKLLTPEVKSRDHTGWRNWIEGQHNRVLRAALEDRPEWPEPFSII
                     KTISEVTDHEAVITTDVGQHQMWAAQHFGVRHGHRWITSGGLGTMGFGLPSAIGAKMG
                     RPDLEVWTICGDGGFQMSGNEMATAVQEGLDINIAIINNGYLGMVRQWQDLFHARNYS
                     EVEISAPNFVKFAEAFGATGIRVTRHEEIRPAIERARSIKGPVLIDFVVEPEANVYPM
                     VAPGASNSDMIQDPQWSPSPEDV"
     gene            4243..4782
                     /gene="ilvN"
                     /locus_tag="VGR29_08240"
     CDS             4243..4782
                     /gene="ilvN"
                     /locus_tag="VGR29_08240"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV2073614.1"
                     /translation="MEQSHTLVLLVEDHPGVLNRVVSLMRRRSFNIDSITVGHSEQPG
                     ISRMTIQLRGEAADVEQAGKQLYKLLEVIKVVDLSPNEAVIRELVLVKVAAKGAVRAE
                     VVQTASIYGAKVLDATPSSLMIEMVGREDEIESFLAMMRVYGIREMVRSGRVALARVA
                     PAKRGHVDRITDTPYAVAD"
     gene            4913..5947
                     /gene="ilvC"
                     /locus_tag="VGR29_08245"
     CDS             4913..5947
                     /gene="ilvC"
                     /locus_tag="VGR29_08245"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV2073615.1"
                     /translation="MPVTIYYENDATLAPLQDKTVAIIGYGAQGHAHALNLRDSGLSV
                     VVGLHEGSKSRAKAEAEGLRVESVEDAVNESDVVMVLVPDHLQKSIYEASIEPNLTAG
                     KMLMFAHGFAIHFGAITPSPDVDVSMIAPKAPGSRVRELYQEGIGVPALVAVHQDPSG
                     NAKETALAYARGLGSLNAGVLETTFKEETETDLFGEQAVLCGGVSALMQAGFETLVEA
                     GYAPESAYFECVHEMKLIVDLIYEGGLTYMRQVVSDTAEYGDYKAGPKIITDQTKAAM
                     KELLSEIQDGSFAREWIAENEEGRENFLALRQANEGHEVEQVGAELRGMMPWLKTGKA
                     AREATTAKRG"
     gene            6019..>7570
                     /locus_tag="VGR29_08250"
     CDS             6019..>7570
                     /locus_tag="VGR29_08250"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV2073616.1"
                     /translation="MSIREGNVADGRDRVIVFDTTLRDGEQSPGATLTWEEKLEVADA
                     LLALRVDVIEAGFPAASPGDFEAVREIARRSRGSGVTIAGLARASRGDIERAAQAVQE
                     ADLPRIHTFIATSDIHLRHKLRMTREQVLDRVRDSVTFARSFVEDVEFSCEDATRSDR
                     EFMAEVFNVAVEAGATTLNIPDTVGYTTPFEYYELITYLKENIARSDEVILSVHCHDD
                     LGMATANTLMAVKAGARQVEVTMNGIGERAGNTSLEEVVMALSTRSEEFGGVQTRIEK
                     QRLVPTSRLVSMLTGIEVQVNKAIVGSNAFAHEAGIHQDGMLKERTTYEIMNPADVGW
                     EGTSLVLGKHSGRAGFRNALKDLGIKLEEAQVQIVYERFLELADRKKKITGADLEALV
                     ADQVSSKSGTLRLVRWSANIGTGGSATASAVIGRGDQEISGDAEGNGPVDALMKAVDA
                     ALGVENELTYFHVEAVTPGEDAQGQVHVRIKHEGQSYSGHGLATDIVEASAKAYIAAL
                     SDVAAHHPD"
BASE COUNT         1598 a         2079 c         2502 g         1391 t
ORIGIN      
        1 gttcctcagc atgaacagcc aggagcgcaa tggattgatc cgtctggtca tggaaggtca
       61 cctgcgtggc atcactggcc agctcacggt tgaggcgatc gtgaaagagc cggagatggt
      121 tgctgaccgg atgcgagcta acgtcgcaga ggatatggca aagatggggc tcgaggtggt
      181 gtcgttcacc atccgcgagg tcaaggattc caatgaatat attctgaaca tgggcaaacc
      241 ggacatcgct aatgtgaggc gcgtggcgga tatcgcgacg gcagaagccg accgcgatac
      301 agcgattcgg cgggcacagg cgagccgtga ggcccggatt gccgaagccg ccgccgagca
      361 ggagaaggtg acggctgaat cgatgagcca gaccaagcag gccgaggccg tccgcgatct
      421 cgagctgaag cgggcggagt tcgaccgctc ggtacaggag cagcgggcgc aggccgacaa
      481 ggcttacgag atccaggcga gtgtgcagca gcagaggatc atggctgagc aggtccgcat
      541 cgagcaggtc cgaaaggaag gcgagatcgc ggttcaggaa gcggagatca ttcgtcggga
      601 gaaggaattg atcgcgacgg tgctccgggc cgccgaggtc gagaagaagc gaatcgagac
      661 cttggctgag gcagaccggc agtgtcaggt tttgcgagca caagcggtgg ccgagcagac
      721 ccggctggag ggagaggcgc aagccgagat cgtccgcatc cagggtcaag ccgaggcgga
      781 tgtgattgcg gcgaagggcc agtccgaagc gcatgccatg cagatcaagg cggcggcgtt
      841 ccaggagtac aaccaggccg ctgtcctgga caagttgatc tcccagatgc cggaggtcgt
      901 gcgcgccatc tccgagccgt tgaacaaggt ggacaagatc acgatcgttt ccactggggg
      961 tgacgggcag aacggcacga ccggagtaaa ccgtctggcc ggagatatga cgtcgcttgt
     1021 ggcacaggtg ccagcattgc ttgagtcgct gacgggagtc aaactggagg acctcctgaa
     1081 gcacgtgccg gaaacaggtg atacttatca ccggagcggc gagaacgctt caccggcacg
     1141 gaagaccgac aggagggatt ccccgatgtt ggtggtcgac gccgagccca tgaaagccga
     1201 gaatggcaag gcccggcgac cgtcgaaaga cgggcaaggg tcgcaagaga gtccggaata
     1261 gggtcgcgta tcctttccat ggatatccgt gcaccggtta cgacccctga gcagcgttag
     1321 cgaggaagat ggcggacaac atggaaccga gcgccccggg tggcgacgcg cagttgcttc
     1381 aggtagaggc tctcggtgag ccgggtcacc ggcgctttcg catcgttgcg gtgatcgaaa
     1441 gtgagaccta tatcctctgg atggaaaagc agcaggtgca ggcgcttggc ctcgcgctag
     1501 agcagctcat cgagcaagtg cctggatccc ctggagctgg tggggtcggg aacgcctttg
     1561 ccatcgtcaa tgaggagaca cgaaaccagt ttcgggtagg tcggatagag attgcttttg
     1621 acgatctcat tgagcaaatc ctcatctcgg cgcacgacat tgagcaggat acgccggacg
     1681 ctccgcccac gctcgcgatc cgtatctcac gcgagcaggc acgggaactg agcaccgagg
     1741 cggcggatct ggtagcgaga ggccggccca tttgcccaat gtgtggatcc ccaatggagc
     1801 gagagggcca cgtctgcccc cagcagaacg gtcatttacc gctcgccttt ggcgatgagt
     1861 tgacgtaaca atctacacag gtggcaattg ctcagatcgt tgctacctgg cggtcggctt
     1921 ttgcggcggg tcaatcagga caacacgttc gccacgcacg gcggtggatc aagttgagcg
     1981 acatgccgtc gcaagattcg ctgaaaggtg ttcccggccc atgcgcgttt gcctggggtt
     2041 gacaccttcc gcagggacac gtaccataaa agaggcgcgg gatgaccgcg tgaagaattg
     2101 cacgaagtga aaccatgcag acgtctatct tctcgcttcc cattattagc ctcgtactta
     2161 ttgagctccc agtagggggc gatttgtgtg ggcgagggca gcgggccgaa taaggcaaga
     2221 gaacgagacg atacgcatgc tggcctcccc caatgggagg cttttttgat gctcctgacc
     2281 aacgacgcgc gggaagcatc cgattcctcc ccatcagtcc tgacgcaggg acccgggggc
     2341 agcagctcag ttcgacggcg acgttgtttg ggatgctgaa gactgcaacg atagcggtcg
     2401 aatatcggag tgcagattcg tgtcgctgac gccgttaacc atagccggtg acggagggaa
     2461 aaggagcaca atgatggtag ccgtggaatc ccggtccctg accggggaag tacctgccca
     2521 atctccgatc cagaccaggc ccgagattct ctccggggcg cagattgtct gccgcagcct
     2581 cgaagaggag ggggtcacga cggtattcgg ttacccgggc ggagcggtca ttccactgta
     2641 tgatgctctc cctcccacga acctccacca tgtcctgacc cggtttgagc aatgggcggg
     2701 tatggccgct atcggatacg ctcgcgttac tggagaggtc ggcgtctgta ttgccacctc
     2761 gggccccggc gccacgaatc tggtcacggc tctcgccgac gcgatgctcg actctgtgcc
     2821 gcttgtcgcc attaccgggc aggtcatgca gggcctgatc ggacgtgatg gcttccagga
     2881 agtggatatc accggcatca cgctcccggt taccaagcac aactacctca ttcgccgacc
     2941 ggaggatatc gcctcaaccc tgaaggaagc cttctacctg gcccgctccg ggcgcccggg
     3001 accggtgctt gtcgacatcc cgaaggatgt cttcatcgcc aaggccccct atacctatcc
     3061 ggagacgacc gggcgcaaga gctatcaacc gaacctcgtc ccaaacatgc ggcaggtccg
     3121 gttggcggcg gagatgatca attcagccaa gcgaccgctc atcatggcag gtcacggaat
     3181 tatcctgagc ggtgcggaag cggcgttccg ggatttcacg gagaaggccg gcattccggt
     3241 cattttcacc ctgctgggac tgggcgccct ggatgaggga catccactct ctctgggcat
     3301 gatgggcatg catggccacc gcgcggcgaa ccgggcgctt gatgaatgcg acctgctcgt
     3361 gaacttcggc gcgcgctttg atgaccgtgc gacaggaaac ctcagcggat ttgccccgaa
     3421 cgcgaaggtc atccacgtcg acattgatcc ggccgagatt ggcaagaacg tgccgacgga
     3481 tgtgcctgtg gttggcgatg ccggcgaagc gctgaaactt ctcacccccg aggtgaagtc
     3541 tcgcgatcac accggttgga gaaactggat cgagggacaa cacaaccgtg tgctgcgagc
     3601 ggcactggag gatcggccgg agtggccgga gccgttctcc attatcaaga cgatctcgga
     3661 ggtgacagat cacgaagcgg tgatcacgac ggatgtagga cagcaccaga tgtgggcggc
     3721 acaacacttc ggcgtgcggc atggacaccg ctggatcacc tccggtgggc tcggcacgat
     3781 gggatttgga ttgccatcag ccattggtgc aaagatgggc cggccggacc ttgaggtctg
     3841 gacgatctgc ggcgatggcg gttttcaaat gtccggtaac gagatggcca cggcagtgca
     3901 ggaaggcctg gatatcaaca tcgccatcat caataacggc tatctcggca tggtccgcca
     3961 atggcaggac ctcttccacg cccgcaacta ctccgaggtg gagatcagcg cgccaaactt
     4021 cgtgaagttt gccgaagcct ttggcgcaac gggcatccgc gtgacgcgcc acgaggagat
     4081 ccggcccgcc atcgagcggg cacggtcgat caagggtccc gtgttgatcg acttcgtggt
     4141 tgagcccgaa gcaaacgtct accccatggt ggcacccgga gcgagcaatt ccgacatgat
     4201 tcaggacccg caatggtcgc cgtcgccgga ggatgtctag tcatggaaca gtcacacaca
     4261 cttgtcctgt tggtggagga ccatcccggt gtcctgaacc gcgtggtttc gctaatgcgg
     4321 cgacggtcgt tcaatatcga ctcgatcacg gtaggacact cggagcagcc ggggatctcg
     4381 cgcatgacca ttcaactgcg cggtgaagcc gccgatgtcg agcaggctgg caagcagctc
     4441 tacaagctgc tcgaggtgat taaggtcgtc gatctctccc ccaacgaagc cgttattcga
     4501 gagctagtgc ttgtgaaggt ggccgcgaag ggtgcagtga gggccgaggt cgtgcagacg
     4561 gcgtcgatct acggggccaa ggtgctcgac gcgacgccaa gcagcctgat gatcgagatg
     4621 gttggacgcg aggacgagat tgaatcgttc ctggcaatga tgcgcgtcta tggcattcgt
     4681 gagatggtac gaagcggcag ggtcgcgttg gctcgggttg cccctgccaa gcgcggtcat
     4741 gtcgatcgaa ttaccgacac gccgtatgca gtggcggact agcaaatcgg ggaagggagc
     4801 gcttacacgc tcccttcctt tctgcaatgg attcttgctt gagtgacgac cagggtggcg
     4861 tcacatgata gagatgggcg accttgcggt cgcggtacgg aggaaacggt agatgccggt
     4921 aacgatctat tacgaaaacg atgcaacact cgcgccgctc caggacaaga ccgttgccat
     4981 tatcggctat ggagcacagg gacatgcgca cgccctgaac ctgcgcgatt ccggccttag
     5041 cgtggtggtt gggcttcacg agggaagcaa gagccgtgcg aaggctgagg ccgaaggctt
     5101 gcgcgtggag tcggtcgagg acgcggtaaa cgagtcggac gtcgttatgg tgctcgtccc
     5161 tgatcacctg cagaaatcga tctacgaggc atcgatcgag ccaaatctca ctgccggcaa
     5221 gatgctgatg ttcgcgcacg gtttcgcgat ccatttcggt gcaattacac cgtctcctga
     5281 tgttgacgtg tcaatgatcg cgcccaaggc accgggttcc agagttcgcg agctttacca
     5341 ggagggcatt ggtgtaccgg cacttgttgc ggtgcatcaa gatccgtccg ggaacgccaa
     5401 ggagacggcg ctggcatacg cgcgtgggct gggttccctg aacgccggtg tgctggagac
     5461 gaccttcaag gaagagaccg aaactgatct ctttggcgaa caggccgtgc tgtgcggtgg
     5521 cgtttcggcg ttgatgcaag ccggtttcga gaccttggtc gaggctggat acgctccaga
     5581 gagcgcgtat ttcgagtgcg tacatgagat gaagctgatc gtggacctca tttatgaggg
     5641 cggcctcacc tacatgcgac aggtcgtctc cgacacggcg gagtatggtg actacaaggc
     5701 gggcccgaag atcatcaccg accagaccaa ggcggcgatg aaggagctgc tttcggagat
     5761 ccaggatgga tcgttcgctc gcgagtggat cgccgagaac gaggagggcc gcgagaactt
     5821 cctagcgctc cggcaggcga acgagggaca cgaagtcgag caggttggtg cagagctacg
     5881 cggcatgatg ccgtggctaa agaccggcaa ggcggcccgc gaggccacca cggcgaagcg
     5941 cggataggcg cgataaaccg cacgtgacgg atgctcgtcg cttttggtcg acgagtagct
     6001 gtcggagaga gtagggcaat gagtattcga gaaggcaacg tagctgacgg gcgggatcgc
     6061 gtcatcgttt tcgatacgac gctgcgcgac ggggagcaat ctcctggcgc aacgcttacc
     6121 tgggaagaga agctcgaagt cgcggatgcg ctccttgccc tgcgcgtcga tgtcatcgag
     6181 gccggattcc ccgccgcgtc tcctggtgat ttcgaggcgg tgcgcgagat cgcgcgtcga
     6241 tccaggggat ccggcgtgac aatcgcgggg ctcgcccggg cgtcacgtgg agacatcgag
     6301 cgtgccgcac aagcggtgca agaagccgac ttaccgcgca ttcatacgtt tatcgcgacg
     6361 tccgatatcc atctcaggca caagcttcgg atgacccggg agcaggtgct ggaccgggtg
     6421 cgtgactctg tcaccttcgc caggagcttc gttgaggatg tcgagttctc ctgcgaggat
     6481 gcgactcgat ccgacaggga gttcatggct gaggtcttca acgtcgcggt tgaagctggc
     6541 gcaaccacgc ttaacattcc tgacactgtc gggtacacca ccccgtttga gtactacgag
     6601 ctgattacct acctcaagga gaacatcgcc cgtagcgatg aagtcatcct ctcggtgcac
     6661 tgccacgacg acctcggtat ggcgactgcc aatacgctga tggctgtgaa ggcgggagcg
     6721 cggcaggtcg aggtgacgat gaatggcatc ggtgaacggg ccgggaatac gtcacttgag
     6781 gaagtcgtga tggctctctc cacccgctcc gaagagtttg gtggcgtgca gacccgcatc
     6841 gaaaaacagc ggctggtgcc gaccagccgt cttgtgagca tgctcaccgg catcgaagtg
     6901 caggtgaaca aggccatcgt tggttcgaat gcgttcgcgc acgaggctgg tatccatcag
     6961 gatggcatgc tgaaggagcg gacgacgtac gagattatga atcctgccga tgttgggtgg
     7021 gagggcacca gtctggtgct tggcaagcac tccgggcggg ccggattcag gaacgccttg
     7081 aaagatctcg ggatcaagct cgaggaggct caggtccaga tcgtttacga gcgcttcctg
     7141 gagctggctg accggaagaa gaagatcacg ggagcggacc ttgaggcgct ggtagctgac
     7201 caggtttcct cgaagtccgg tacgctccgg ctcgtgcgct ggagcgcgaa tatcggtacg
     7261 ggcggctccg ccactgcctc agccgttatc gggcgcggcg atcaggaaat ctccggggat
     7321 gctgagggca acggtccagt cgacgctttg atgaaggcgg ttgacgcggc gctcggcgtc
     7381 gagaacgagc tgacgtactt ccatgtcgaa gcggtgactc ccggtgaaga tgcccaggga
     7441 caggtgcatg tgcgcatcaa gcacgagggc cagtcctact cggggcacgg cttggcaacc
     7501 gatatcgtgg aagcgagcgc gaaggcgtac atcgcggcac tgagtgacgt tgcggcgcac
     7561 catccggaca
//
LOCUS       DASYWM010000203         6172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_552674, whole genome shotgun sequence.
ACCESSION   DASYWM010000203 DASYWM010000000
VERSION     DASYWM010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552674"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..770)
                     /locus_tag="VGR29_08255"
     CDS             complement(285..770)
                     /locus_tag="VGR29_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073617.1"
                     /translation="MRTTPIRTPRPQRIEAPAGCRECGVVLEDRTRHYCDECLPEYRE
                     AQVTSYAEAGRAKPQEMREAGGDPSQTGEAATKRRDTMTQRRREEAAWDEAHPDVKVD
                     EAVFRSKILPGLQGVPLSTLVTVTGLSQQYCSLARRGLKTPHPRHWKRFSSVTVSSMH
                     D"
     gene            complement(821..1354)
                     /gene="cas1"
                     /locus_tag="VGR29_08260"
     CDS             complement(821..1354)
                     /gene="cas1"
                     /locus_tag="VGR29_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HEV2073618.1"
                     /translation="MRSVEDHAAAAYWSAWTPATIRFAKRDQAKVPEHWKTFGTRSSP
                     ISGATRNASNPLNALLNYVYAILETEVRLAILTMGLDPGMGILHADLKSRDSFVFDVI
                     EPLRSEVDGYLLTMLEERTFAAREFFETRQGCVGSCRRCRRSSPRWHRVWPSLWLRWW
                     SRWRSGSPKDRERPRDP"
     gene            complement(1610..2095)
                     /locus_tag="VGR29_08265"
     CDS             complement(1610..2095)
                     /locus_tag="VGR29_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073619.1"
                     /translation="MQQKQAALRGLVAGIAGVAAMTAAEKLEQQFTHRPNSYVPAHTL
                     ARLVRRKLKPDEQHHGLNWTMHWGQGILLGAVRGLMARRGIRGPVGSFLFLNLRLLND
                     QSLENATGVGAPPWTWPVDEQVIDLAHKAVYAFATGLVADRLIGGPKRVPGPRRPWRQ
                     R"
     gene            2471..3538
                     /locus_tag="VGR29_08270"
     CDS             2471..3538
                     /locus_tag="VGR29_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV2073620.1"
                     /translation="MLSSPDLPHLGPLRLPERSPVPALVRRILLALGLVLLVVAVIWF
                     DREGLRDHAHEGRPLGITDVLYFTVVSLTTLGYGDISPVTTEARLLNALLLTPIRIFL
                     WVLFLGTAYEMSVLRLQLREERHMRELHERLKDHVVICGYGVKGRAIVSELVAHDHER
                     ENIVIIDPSEEAVAAAAREGLVALRGDASSESLLQAAAIERAAYVLAAPDRDDACVLI
                     CLTVRNLAPTVQLVAAAREEENIKLLYGAGANLVVAPSVSGGRLMASAVRQQAVPHFL
                     EDVLAFGEGLAVAEREVRSDERGLLAHDLPDLATSLILGVARGTERCPFYQLSDYVLQ
                     PGDIVVYLAGDPNCRSQGRPA"
     gene            3630..4160
                     /locus_tag="VGR29_08275"
     CDS             3630..4160
                     /locus_tag="VGR29_08275"
                     /inference="COORDINATES: protein motif:HMM:NF020022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2073621.1"
                     /translation="MAEVILTRGLPASGKSTWAKAQLDAHPGQFKRVNKDDLRAMLDN
                     GRWSKQNEKLVLQVRDDIIRTSLGRGCHVIVDDTNLSPRHERRIRQLVEEFNKEHGES
                     HEVCIRDFTDVRLETCIERDAKRDQPVGEKVIRGMYRQFLAPVAEPAHQVEVAVAASG
                     NELEAHGPGSATEVEQ"
     gene            complement(4291..4914)
                     /locus_tag="VGR29_08280"
     CDS             complement(4291..4914)
                     /locus_tag="VGR29_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073622.1"
                     /translation="MTKRFFAPVTLLIAAFVLALGVAASLSAASAQGTATPAGQEDVD
                     PVAPYPAHIHSGTCEELGDVVFPLSDLTRIGADVPPGGTPVEGVPTVAVDLDATPVPG
                     AVDETTIVSGSTTIVEASLDDILAEEHAINVHQSPENIDVYIACGDLTGTPTDGELQI
                     ELQELNESGYVGEAQLQDNEDGTTTVTVVLMLGAEDGTGTPEASPAG"
     gene            5264..>6172
                     /locus_tag="VGR29_08285"
     CDS             5264..>6172
                     /locus_tag="VGR29_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV2073623.1"
                     /translation="MTDTSQREAMEDSSSILRAGRREQRSGVALCLSGGGFRAALFHL
                     GALRRLNELGILSDVDTISAVSGGSILAAHLVERVPDWPNRGEIVQDWEERVAEPFRV
                     VTSKNLRTGPVLRRLLPWNWGRPQSGVQALARRYQTEITRARLGDLPDRPRYLFCATD
                     MVYGRFWVFEKRGAGRWDADLSPRPKWPIARAVAASSCFPPIFAPMEVGLSPQQDGGD
                     GSGHARPPKRHVDQSHLTDGGTYDNTGVEAVWEDHATILVSDGGGTFDTRWRNSWLWR
                     LRRYPSIASARVRVVQKRWLFASFRAD"
BASE COUNT         1092 a         1887 c         2015 g         1178 t
ORIGIN      
        1 gcgtcaacag tcgctgtcgc gctctatcga ggcgtggagt tctaccagta tctctacggt
       61 gccgacagcc tcgatggcga cgagtgggac cccagggatt tgatggaggg ccatgacgaa
      121 gcgggcggcg ccatcgtcga cgtcgcgttc gaagtcgcta agcagctggc gtagaaggct
      181 gttctctttg aggagtcagc gttcggtatg gagtgggtac tgcggataat ttacgttgtt
      241 agaagcgtca ttggttcatt gagagctctc gcttccagcc agcctcaatc atgcatggac
      301 gagacagtca cagagctgaa acgcttccaa tgccggggat ggggggtttt cagaccacgc
      361 cgggcaagtg agcagtattg ttgagataat ccggtcacgg tcacgagggt cgaaagcggc
      421 accccttgga gccccggcag gatcttgctc cggaagaccg cctcatccac cttcacgtca
      481 ggatgcgcct catcccacgc cgcttcctcc cgccgtcgct gcgtcatcgt gtcgcgacgc
      541 tttgtagcgg cttcccccgt ttgcgacgga tcaccccccg cttcccgcat ttcctggggc
      601 ttcgcccgtc ccgcctcggc ataagaggtc acctgcgcct cccgatactc cggcaggcac
      661 tcgtcacagt agtggcgcgt tcgatcctcg agcaccacgc cacactcacg gcacccggcc
      721 ggtgcctcga tccgctgcgg tcggggagtc cgtatcggtg tcgttctcac tcgatcgcgc
      781 cccttactgc gattgctttc ggtcaagagc gttggcaccg tcaagggtct cgcggccgtt
      841 ccctgtcctt cggagagccg ctgcgccacc tgctccacca ccggagccac aagcttggcc
      901 agacgcggtg ccatctcggc gaggacctgc ggcagcggcg gcatgagccg acacacccct
      961 gccgggtctc gaagaactcc cgggcagcaa aagtacgctc ctcgagcatc gtcagcagat
     1021 acccatccac ctccgatcgc agcggttcga tcacatcaaa gacaaaggaa tcccggctct
     1081 tgagatcggc atggaggatc cccatgccgg ggtctagccc catggtcaga atcgccaggc
     1141 ggacttccgt ctcgaggatg gcatagacgt aattcagcaa ggcattgagc ggattactcg
     1201 cgttgcgggt ggcaccggag atcggggagg agcgggtgcc aaaggtcttc cagtgctctg
     1261 gcaccttcgc ttgatcccgt ttggcaaacc ggatggtcgc cggagtccat gccgaccagt
     1321 aggcagcggc cgcatggtcc tcgactgagc gcagatcccg atgttggcgt catgatgcct
     1381 gggggccgca ccgaggatgt cagcgtcccg aggcggggga acgtcagcac cccggtggcg
     1441 tagcacgacg atatttcggc aggagggttt gtggcgcacc atcctgcttg tcgagtgatt
     1501 ttcggaggcc aactaagagg tcagcctcgg gttctcaggg ctgcgctgcc gctccaacat
     1561 gggaaacatg cgcacctggc cctggtgttg agttagaggc ggggcgaggt taccgctggc
     1621 gccatggtcg ccgtggtccc ggcactcgct ttggtccgcc tatgagcctg tcggctacca
     1681 ggccggtggc gaaggcgtag accgccttgt gggcgaggtc gatcacctgc tcgtcaacgg
     1741 gccaggtcca tggcggtgca ccgacacccg tcgcattctc cagactctgg tcgttcagca
     1801 gccggaggtt aaggaagagg aaggagccaa ccggtccccg tatccccctc cgggccatca
     1861 gtccccggac ggcaccaagc aggatcccct gtccccaatg catcgtccaa ttgagaccgt
     1921 ggtgctgctc gtccggtttc agtttccggc ggacaagacg cgcgagcgtg tgggccggca
     1981 cataggagtt cggccgatgc gtgaactgtt gctccagttt ctcagccgca gtcattgcag
     2041 caacgccggc gatgccagcc acaagtcctc gcagtgccgc ctgtttctgt tgcatcgctg
     2101 catccttttg atgaagaggc aagagcgggt taatgccccg tgaaccttct ggcagttacg
     2161 gacggtgccc aggggtcggg agtgacgtat acggaatatc cggcgtgacc ccggtgtgtc
     2221 gccgaggtag gcaagaactg ggccaggtcg ggtgtgcggc gctctgtcgt ggagatgcgg
     2281 cgcgaggcgg atgccggggt caatactgga gacttcggct atggactgaa ctctatcggg
     2341 attcgaggcc aatcggtaca cggcggagcc cggcagcgtg catgtattct ttccaagggg
     2401 agagggggaa gccgtcgcgg catcgcccgc ccagtttccc gcctgaggta ttcgaggatc
     2461 tgcgccgcgc atgttgtctt cacccgacct tccccatctc ggcccgttgc ggctgccaga
     2521 gagatcgcct gtgcccgcgc tcgtgcggcg catcctgctg gccctgggcc tggttctgct
     2581 cgtggttgcg gtcatctggt tcgaccgtga aggcctgcgc gaccacgcgc acgaggggcg
     2641 gccgctcgga attaccgatg ttctctactt cacggtcgtt tccctcacca cccttggcta
     2701 tggcgatatc tcaccggtga caaccgaagc gcgcctgttg aatgccctgc tgctcacgcc
     2761 gatccggatc tttctctggg tgctctttct cggaacggcg tatgagatga gcgtccttcg
     2821 cctgcaactt cgagaggagc gtcacatgag agagctgcac gagcgcctca aggaccacgt
     2881 cgtgatctgt ggttacggcg tcaaggggcg cgcaattgtc tctgagctgg tggctcacga
     2941 ccatgagcgg gaaaacatcg tgatcatcga tccgagcgaa gaagctgtcg ctgcggcggc
     3001 acgggagggg ctggtcgcgc tgcgtgggga tgcctcaagt gagtcgcttc tgcaagcggc
     3061 ggcgatagag cgggcggcgt acgtcctggc tgcgcccgac cgggatgatg cctgcgtgtt
     3121 gatctgtctc acggttcgca acctggcgcc gacggtccag cttgtcgccg ccgcgaggga
     3181 ggaagaaaac atcaagctcc tctacggggc aggagcgaat ctcgtggtgg ctccatcggt
     3241 gtcgggagga cggttgatgg cctcggccgt gcgccagcag gccgttccgc acttcctgga
     3301 ggatgtactg gcattcggtg aaggactcgc cgttgccgag cgcgaagtac ggtcggacga
     3361 gcgtggtctc ctggcacatg acctcccgga tctggcaact tcgctgatcc tcggggtggc
     3421 acgggggacg gaacgttgtc ccttctacca gctctcggac tatgtgctgc agccgggcga
     3481 catcgtcgtc tatctcgccg gcgatccgaa ctgccggagc cagggcaggc ccgcctgacc
     3541 ccgcgctccc tgccatgctg gcgggctgtc ccatcccggg acatcggttg cattcgccgg
     3601 ggccaaaagg ccaggtaagg aagaggacca tggcagaagt cattctcacc aggggattgc
     3661 ctgccagtgg caaatccaca tgggcgaagg cgcagctcga tgctcacccc gggcagttca
     3721 agcgtgtcaa caaggacgac ttgcgggcaa tgctcgacaa cgggcgatgg agcaagcaga
     3781 acgagaagct ggtgctccag gtcagggacg acatcatccg gacgtccctg ggacgcgggt
     3841 gtcacgtcat cgtggatgac acgaacctct cgccgaggca tgagcggcgg atccgccaac
     3901 tcgtcgagga gttcaacaag gagcatggcg aaagccatga ggtctgcatc agggatttta
     3961 ccgacgttcg gctggagacc tgcattgagc gcgatgcgaa acgcgaccaa ccggtaggag
     4021 agaaggtcat ccgggggatg tacaggcagt tcctggctcc ggtggccgag cctgctcacc
     4081 aggtcgaggt ggccgtggca gcgagcggta acgaactgga ggcgcacggg ccgggctcgg
     4141 cgaccgaggt ggagcaatag ctgcggtacc gcgatctggc cgcgggaatg tcggctcgcc
     4201 gctgtcagtc ctgatggaca gggcaaagag caacggccaa aggttgcccc atgaccgtta
     4261 ctcctgatga tccttttaag acacgtaatc ctagcccgcc ggagaagcct ctggtgttcc
     4321 ggtgccgtcc tccgcgccga gcatgagcac gactgtcacc gtcgtagtgc cgtcttcgtt
     4381 gtcctgaagc tgagcctcgc caacgtagcc ggactcgttg agctcctgca gctcgatctg
     4441 gagctcaccg tccgtcgggg tacccgtgag atcgccgcag gcgatgtaga cgtcgatgtt
     4501 ctcagggctc tggtgcacgt tgatcgcgtg ctcctcggca aggatgtcgt ccagggatgc
     4561 ttccacgatt gtggtgctcc cggacacaat cgtcgtttca tcgactgctc ccggtaccgg
     4621 tgtggcgtcc aagtcaaccg ccacagtagg cactccctcg accggcgtgc cgcccggagg
     4681 gacatcagca cctatccggg tgagatcgct cagcgggaag acgacatcgc cgagctcctc
     4741 acaggttcct gagtgaatat gcgcggggta gggtgcaaca ggatcaacgt cctcctggcc
     4801 ggcaggggtc gctgtcccct gggcacttgc cgccgagagg cttgccgcaa cacctagcgc
     4861 caggacgaat gccgcgataa gaagcgtcac tggtgcgaag aagcgtttgg tcataaccga
     4921 tcctccttct ctataagagt ggactcgaaa ccgaatggct ccatccggaa ttaccaatgc
     4981 tgcacgagct gtgccacatg cgatcgacgg gcgggccgat tcatcgagcg tcggtcgcgg
     5041 atagactgtc aggttccggg gccggcggac tggtccgcaa aggagcggcc ggagcgtggc
     5101 ggcaaagtat tccccgtcgc ccctgcccga gatggaggag atacgccggg gtgcaacggg
     5161 cagggcaggg cagggcagga acggatcagg gcggggtggc accacgcttg cgcgcttcgg
     5221 gacagcagat gacgcaaagc caaagctatg ggaggagggc agagtgacag acacgagcca
     5281 gcgggaggcg atggaagatt cttcgtccat cttgcgtgca gggcgacgag aacagcgtag
     5341 tggggttgcc ctttgcctct ctggtggtgg ttttcgtgcc gcgctcttcc atctcggcgc
     5401 tctgcgccgt ctgaacgagc tcggcattct ctcagatgtt gacaccatat cagccgtctc
     5461 gggcggcagc attctcgccg ctcatctcgt cgagcgcgtt cccgactggc cgaacagggg
     5521 tgagatcgtg caggactggg aggagcgggt ggccgagccc ttccgggtcg tcacgagcaa
     5581 gaatcttcgc accggccctg ttctccgccg actgcttccc tggaattggg gtcgcccgca
     5641 gtccggggtt caggcgctcg cccgccgcta ccaaactgag atcacccgag cgcggctcgg
     5701 cgatctgcca gaccgcccgc gctacctctt ctgcgcaacc gatatggtct atgggcgctt
     5761 ttgggttttc gagaaacgcg gcgctggccg gtgggatgcc gatctttcgc cgcgtcccaa
     5821 atggccgatc gcgcgggctg tcgccgcctc ctcatgcttc ccgccgatct ttgccccgat
     5881 ggaagtgggc ctgagcccgc aacaggatgg cggagacggg tcgggacatg cccgtccccc
     5941 gaagcgtcac gtcgaccaga gccacctgac cgatgggggt acctacgata acaccggggt
     6001 tgaggcggtc tgggaggacc acgcgaccat tctcgtctcc gacggcggcg ggaccttcga
     6061 cacccggtgg cgtaactcct ggttgtggcg cctccgccgg tacccatcga ttgcgagtgc
     6121 tcgagtgcgg gtggtccaga aacgatggct cttcgccagc ttccgcgccg at
//
LOCUS       DASYWM010000204        13273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_346030, whole genome shotgun sequence.
ACCESSION   DASYWM010000204 DASYWM010000000
VERSION     DASYWM010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13273
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346030"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="VGR29_08290"
     CDS             <1..1495
                     /locus_tag="VGR29_08290"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2073624.1"
                     /translation="PTTVDDHVWYQLEGEAGFGWVAASTFGPSNAASPAGKFKFGEEL
                     VMNTDVINIRETPSLKGLISATLPYMAKAVVVSGPMPANGYKWYQVTTDYGAGWSVEQ
                     YLAREGEVTPPTRFVVGDAVQVGDPEGIRLRSGSSSQSTPIASLSPGVRGVVIEGPTA
                     ADGFVWLKIQTSQGSGWCAEEFLDEAPSADPPAARFGLGDRVLVDTDSLNLRESPGAT
                     APIVVSLGTGVSANVVGGPETANNMNWYQLETDYGTGWSVEQFLTKEDAASTSRVFSP
                     GSRVYVNTDSLNLRSSPGIDSEVVAVLVTNDRGEIAGGPVEKDGFNWYQVRSDGGTGW
                     GAAQYLGKGTADPFSEKTFSAGDTVYVDTDLLNIRAEASLSATIVTTLGIDESATVSD
                     RPVEADGFGWVNVTSPKGAGWCVADYLAKVSGNGPRIGDTARVFDGELNLRVATSVGS
                     DVIALIPDAAYVEVLEGPVGAESYDWYRVSTSRFGTGWCAGQWLAVV"
     gene            1646..2161
                     /gene="rplI"
                     /locus_tag="VGR29_08295"
     CDS             1646..2161
                     /gene="rplI"
                     /locus_tag="VGR29_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV2073625.1"
                     /translation="MKVVLRQDVPKLGEAGTVQNVSGGYARNYLVPNGLAVYATEGEL
                     KMAAHNLAVKDRKVARQEDQLRSLAEKIEGQSLVFTARAGEAGRLFGSVTAGDVAQQL
                     SEKIGEEIDRRKIVLDEPLRSLGTSRVAVHLVGRLRPEVTITISREDVPEETAPAESE
                     VTEEADVESEV"
     gene            2315..3661
                     /gene="dnaB"
                     /locus_tag="VGR29_08300"
     CDS             2315..3661
                     /gene="dnaB"
                     /locus_tag="VGR29_08300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV2073626.1"
                     /translation="METTVVERLPPHNVEAEQSVLGSLLIDRDAIIKVAAYLKSDDFY
                     IHANGSIYQAVLDLYNKREPTDFITLSDELDRRGLLDDIGGVTYLSSLLNTVPTAVHV
                     EYYGRIVERQATRRRLIDAGTRIVGIGFQDEVETEDALDAAERELFDVSQKRQTKDFQ
                     SISDVIDRYFDQIDYLQQHRGDVVGVPTDYKDLDQLTGGLQRSDLLILAARPSMGKTA
                     FALGVAYGAAVQHGRSVGIFSLEMSADQLVQRLLATETGVDSHRLRLGMIDDTEWDRI
                     SRAFGRLSEANIFIDDSANASIMDVRSKARRLQAEKGLDLLIIDYLQLMSGRRSENRV
                     QEISEISRGLKGLARELNIPVVALSQLSRAVETRSDHRPMLSDLRESGSIEQDADIVM
                     FIYREEKYDENSDKKGIAELIVAKHRNGPVGSINLRFFEQTARFADLELYREPDAS"
     gene            3658..4338
                     /locus_tag="VGR29_08305"
     CDS             3658..4338
                     /locus_tag="VGR29_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEV2073627.1"
                     /translation="MTRSSSSLTAITSSRLPVSIPHAFLTETVINITCMAELQVSLAM
                     FRLVAVLEGEETPIAERMILRDEALNRAMRRDGIVPEPAERILKGLELAVGRGTILRF
                     LATQPSHQAYWYYLDTTVNRATVASMERGALPPPAIIWEGKTAPAISLDPPNAYRLYE
                     QNIGPLTPLIADQITRAVTDYPGDWIEDAITEAVSYNRRSWRYILRILENWQASGRQE
                     QEHYSGNA"
     gene            4377..5180
                     /locus_tag="VGR29_08310"
     CDS             4377..5180
                     /locus_tag="VGR29_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2073628.1"
                     /translation="MKPIGDVIKNIPVSRSGKSPNGSAIRITRKDDVRCPICQDAGYL
                     RADVPPGHPSFGRLFPCQCKRNELDERRAEDLRRLSNLDAFTGYTFDDFDPSTSGTEE
                     AFEAALAFAQEPDHRWLFLSGPCGVGKTHLAVAIATYAMQWHHMSVYFAVVPDLLDHL
                     RATFDPNSGSAYDERFTSIRNAPLLVLDDLGTENATPWAREKLYQIINHRYSEQMPTV
                     ITSNVELRKVDERIVSRMLDHRLTQYVQIDAEDFRRPGDARRVRRTPRR"
     gene            5454..6380
                     /gene="cdaA"
                     /locus_tag="VGR29_08315"
     CDS             5454..6380
                     /gene="cdaA"
                     /locus_tag="VGR29_08315"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEV2073629.1"
                     /translation="MSDLTWLFERLFDVRAILDILVVAFLIYWLLWVAQGTRALSLIR
                     GIITLFLFLYLLGDGLQLTTLNRILSVIWPALIIAIPVIFQPELRRAFEQLGHAGSWL
                     RSPFPAQDDQAAESMIDEIVRASHQLSRLRYGALIVIERETGLQDFVSKGVQLNADIS
                     RQLLINIFFPNSPLHDAAVVIGDNRILAASVVLPLTDNISASSQLGTRHRAAIGITEE
                     SDSLAVVVSEETGQMSVAHSGRLIRNLDQDRLRRVLRTLMRVDRPVQNGRLDGRFGRL
                     RALIPAASDSRLADGSAGGTRRERRQESIHQP"
     gene            6377..7675
                     /locus_tag="VGR29_08320"
     CDS             6377..7675
                     /locus_tag="VGR29_08320"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEV2073630.1"
                     /translation="MRDHAPSPASPIGALAFRPTRVQVVRFVFSLLIATILWGWVTEL
                     NDPFITVRYRELDVTAGPLDNSLRVVTTLPRATVTLRGPESRVEDISRDEVSVVLDTS
                     EVTEAGEYRLELDVVAPEGPSSKSVNPEELPVTIEQEISEVMPLEVRNAVPRDDPREV
                     TSTVPETTQVTVSGPSSAVGRVEEVILPVSLDNHQSSFEEAFTPYAVDSEEQRVSEVR
                     ILPEEIMTSVDIRSRGKTVSVIPVIEGVPEEGFSVQQRAALPDSIVVEGPDEALESLL
                     FVNTEPVSIAGATQSISSEVGLSNLPPGVTVIEPESGKVEVRVAIEDTTSTAQTLTGS
                     RVEPINLEPGLSAVIEPEGIDVTVDGPGSTLSKMTPSDVKTRVDVSGLRPGTYEVSPE
                     ITVPQGVTWVGNYPQTVQVTIFAVQETPGASPIPQASPGT"
     gene            complement(7837..8496)
                     /locus_tag="VGR29_08325"
     CDS             complement(7837..8496)
                     /locus_tag="VGR29_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2073631.1"
                     /translation="MSRAPQVSTHWRIIIADDESLIRLDLREMLTHLGYEVIGEAGDG
                     RTAVELAKRLQPDLLVMDIKMPDLDGISAAEELTRERIAPVVLLTAYSDQELVERARE
                     AGVVGYVVKPFREAELMPVIELSRARFEEFRTLEREVGGLKDALETRKIVERAKGVLM
                     ETHGLRESEAFHRIRKTSMDARKSMREVAEAILLAHEMDFSIASGAGDTFDGDTSSNN
                     E"
     gene            complement(8718..8794)
                     /locus_tag="VGR29_08330"
     tRNA            complement(8718..8794)
                     /locus_tag="VGR29_08330"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8758..8760),aa:Pro,seq:ggg)
     gene            complement(8914..9300)
                     /gene="rplL"
                     /locus_tag="VGR29_08335"
     CDS             complement(8914..9300)
                     /gene="rplL"
                     /locus_tag="VGR29_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV2073632.1"
                     /translation="MADQAQLIDTLEGLTVLELNELVKALEEKWGVSAAAPVAVAGAA
                     GAAAGGASAAEEEEKTEFDVVLTDIGANKIQVIKAVRELTSLGLKEAKDLVDSAPKAV
                     REGVTKDEAEAARAKLEEVGAKVDVK"
     gene            complement(9381..9923)
                     /gene="rplJ"
                     /locus_tag="VGR29_08340"
     CDS             complement(9381..9923)
                     /gene="rplJ"
                     /locus_tag="VGR29_08340"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV2073633.1"
                     /translation="MPTLKKGAEIDLLVDRLERSQLTIVADYRGLTVSQLQGFRATLR
                     STESEFRVAKNTLVRIAAERVGIEGLESHLDGPTAVLFAFGDIVAPAKAVSDFARTSR
                     ILQVKVGVMNGQVVGPSDIEAISTLPPREELLGKLVGMLVSPMARTVGVLSGPARSVA
                     YLANARIEQLGGATEAQAAD"
     gene            complement(10126..10848)
                     /gene="rplA"
                     /locus_tag="VGR29_08345"
     CDS             complement(10126..10848)
                     /gene="rplA"
                     /locus_tag="VGR29_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV2073634.1"
                     /translation="MGHGKKYLEAAKLVDEEQQYTVEEASELLKRVAFANFDESVELH
                     ARLGIDPRQADQAVRSTVTLPHGSGRQVRVLVFAAGEAERAALEAGADHAGSDELVQR
                     IQGGWLEFDATIAMADQMGKVGGLGRILGRRGLMPNPRSGTVVREVADLSGVVRELKG
                     GRVEFRNDRTGNVHVTVGRKSFSEDQIQANILAVVDALNRNKPSGLKGIYCRTLAIAT
                     SMSPGIRLDVNDTLSKSSNVAS"
     gene            complement(10914..11339)
                     /gene="rplK"
                     /locus_tag="VGR29_08350"
     CDS             complement(10914..11339)
                     /gene="rplK"
                     /locus_tag="VGR29_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV2073635.1"
                     /translation="MAKKIRGYVKLQLPAGKATPAPPVGPALGQHGIALMNFCKEYNE
                     RTQAMAGQIIPVVITVYEDRSFTFVTKTPPAADLLRKAAGIQKGAGNPKTTKVGSVSQ
                     DQVREIAELKLKDLNAVDIEGAIKQVEGTARSMGIEVAS"
     gene            complement(11423..12022)
                     /gene="nusG"
                     /locus_tag="VGR29_08355"
     CDS             complement(11423..12022)
                     /gene="nusG"
                     /locus_tag="VGR29_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV2073636.1"
                     /translation="MERPTLRERLAAKRAEEAGDDGSITQGQWYVLHTYSGYENKVMK
                     TIESRVEAFDLGDRVYEVVVPTQDEIEIKDGQRKTVQRKVFPGYVLVRMEMDEDTWYQ
                     LRNTPGVTGFVSQDNKPVPLKGDAAQVVIQGMEAEAPKVTMSFALGDTVRIIDGPFAD
                     FRGEIDEINNEKGKIKVLVSFFGRETPVELDFLQAEREV"
     gene            complement(12060..12599)
                     /gene="secE"
                     /locus_tag="VGR29_08360"
     CDS             complement(12060..12599)
                     /gene="secE"
                     /locus_tag="VGR29_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV2073637.1"
                     /translation="MSTQDTTAPKRRSSAANRRRKRQADLRQGQAPASQASSVDQLEH
                     APSSMVTEESVRLKPVEESRKESRADRKASAREAVEPGDASELQKEKHLRRKEERREA
                     KSRKPVKEGRRQFVNTERFGGLQRFYEDAASEIRKVQWPDRETTRNLVIVVIALSFVL
                     GVLLGGIDYVLFQLFEALT"
     gene            complement(12755..12828)
                     /locus_tag="VGR29_08365"
     tRNA            complement(12755..12828)
                     /locus_tag="VGR29_08365"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12792..12794),aa:Trp,seq:cca)
     gene            complement(12850..13020)
                     /gene="rpmG"
                     /locus_tag="VGR29_08370"
     CDS             complement(12850..13020)
                     /gene="rpmG"
                     /locus_tag="VGR29_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV2073638.1"
                     /translation="MAKKSKVDRPVVTMECTVCRERNYTTEKNRRNDPGRLELKKFCP
                     RCRCHQLHRETR"
     gene            complement(13132..>13273)
                     /gene="tuf"
                     /locus_tag="VGR29_08375"
     CDS             complement(13132..>13273)
                     /gene="tuf"
                     /locus_tag="VGR29_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV2073639.1"
                     /translation="EMVMPGDNIQMGVELITPVAIEEGLRFAIREGGRTVGAGAVTSI
                     TE"
BASE COUNT         2704 a         4049 c         3722 g         2798 t
ORIGIN      
        1 ccccacaaca gtcgacgacc atgtctggta tcaacttgag ggagaggccg gctttggctg
       61 ggtcgccgcg tccaccttcg gcccgagcaa tgcagcatca ccagccggga agtttaagtt
      121 cggcgaggaa ctggtcatga atacggacgt tatcaatatt cgtgagaccc cgtcgctcaa
      181 aggcctcatc tccgcgaccc ttccctatat ggcgaaggcc gttgttgtga gtgggcccat
      241 gccagccaat ggctacaagt ggtatcaggt caccaccgac tacggtgccg gctggtcagt
      301 ggaacaatac ctggcgcgag agggcgaggt gacgccgccc actcgttttg tcgtcggcga
      361 cgccgttcag gtcggggatc cggaaggaat taggctccgc tccgggtcaa gttcgcaatc
      421 aacaccgatt gccagcctct ccccgggagt tcgtggagta gtaatcgagg gaccgacagc
      481 agccgacgga ttcgtgtggc tgaagatcca gacatcacaa ggctccggct ggtgtgccga
      541 ggagtttctg gatgaggcac cgtcggctga tcctccagcg gccaggttcg gccttgggga
      601 ccgcgtgttg gtggatactg actcgcttaa cctcagggag tcgccgggag caacagcacc
      661 aattgtggtc tcactcggta caggcgtgag cgcgaatgtc gttggaggcc ctgaaacggc
      721 caacaacatg aactggtacc agcttgaaac cgactacggc acgggctgga gcgttgagca
      781 gttcctcacg aaggaggatg ctgcttctac cagccgcgtc ttctcaccag gcagccgcgt
      841 ctatgtaaac accgattcgc ttaacctcag gagttcgcca gggatcgaca gcgaggtcgt
      901 tgcagtgctc gtgaccaacg accgtggcga aattgctggc ggccctgttg agaaggacgg
      961 gttcaactgg tatcaggtca ggagcgacgg cggtacagga tggggagcgg cgcaatatct
     1021 cggcaagggc actgctgatc cattctctga gaaaaccttt tcggccggag acacggtcta
     1081 tgtggatacc gaccttctga atatcagggc ggaagcatct ctctcggcca ccatcgtgac
     1141 cacccttggg atcgacgaaa gcgcgactgt gtcggatcgg ccggttgagg ccgacggatt
     1201 cggctgggtc aacgttacat cgcccaaagg agcgggctgg tgcgtcgcgg attacctggc
     1261 caaagtaagc ggcaacggac cacgcatcgg tgacacggca agggttttcg acggtgaact
     1321 gaacctccgg gttgcgacga gcgtgggtag cgacgtaatc gccctcattc ccgatgccgc
     1381 ctacgtcgag gttcttgagg gcccggtcgg tgcagagtcc tatgactggt accgcgtaag
     1441 tacctccaga ttcgggacgg gctggtgtgc cgggcagtgg cttgccgtcg tgtaagtccc
     1501 tcggcgttgc aggggcgaca gcaaacccgg tagaatgggt tcgacttgca acacgtccgc
     1561 accggttggg tcatcattca aaccggtgtg gtcttttgtg ctgattgcaa actcgttacc
     1621 cgcgatggtt ggaaggtatt agtcgatgaa ggtcgtactg cgacaggatg tgccgaagct
     1681 cggtgaggct ggcacagttc agaatgtttc cggtggatat gcgcgtaact atttggttcc
     1741 gaatggtctc gcggtatatg ccaccgaggg tgagctgaag atggccgctc ataatctcgc
     1801 tgtgaaggat cgcaaggtcg cccggcagga ggaccagctt cgctccctcg ccgagaaaat
     1861 cgagggtcag agcctcgtct ttaccgcccg agctggagaa gcaggtcggc tgtttggatc
     1921 cgtcactgca ggtgacgtcg cccagcaact gtctgagaag atcggcgaag agatcgatcg
     1981 gcgaaaaatc gtgctggacg agcctcttcg ctcactgggc acctcaaggg ttgctgtcca
     2041 tctggttggg cgcttgcgac cagaagtaac cattaccatt agtcgtgaag acgttccgga
     2101 agaaactgcc cctgcagaat ccgaggttac cgaagaggcg gacgtagagt ccgaggtcta
     2161 aaccgaacaa agttgctcat ccccgcacac gagccggccc cgcgttgttt acgacgcgcc
     2221 cggctcgtgg tgtctgtgct gtatcctatg tacgtatatg ttcgtacagt tcgccggacc
     2281 ccgtcagatc gaatgccttt gaggaccgtc ccgtatggaa actaccgtcg ttgaacgcct
     2341 accaccccat aacgtcgaag ccgagcagtc ggtgctcggc agcttgctta tcgaccgtga
     2401 cgcgattatc aaggtcgcgg catacctcaa gtcggacgat ttctacattc acgcgaacgg
     2461 ttcgatctac caggccgtcc tcgacctgta caacaagcgg gaacccaccg acttcatcac
     2521 gctctctgac gagctcgatc ggcggggatt gctcgacgac attggcggag taacctatct
     2581 ctcctccctc ctcaacacgg ttccaaccgc cgttcacgtc gaatattacg gccgcattgt
     2641 cgagcgacag gccacccgcc gccggctgat tgatgcggga acgcgaattg tcggcatcgg
     2701 gttccaagat gaggtcgaga ccgaggacgc gctcgacgcc gcagaacggg aactcttcga
     2761 tgtatcccaa aagcgacaaa ccaaggactt ccagtcgatc tcggacgtga tcgaccgata
     2821 tttcgaccaa atcgattacc tgcagcagca ccgtggtgac gttgtcggcg tccctacgga
     2881 ctacaaggat ctcgaccaac tgacaggagg cctgcagcgc tcggatctcc tcatcctcgc
     2941 cgcccgcccc agcatgggca agactgcgtt cgccctcggc gtcgcttacg gcgccgcagt
     3001 ccaacatggg cgctcagtcg gcatcttcag cctggagatg tccgccgacc agctcgtgca
     3061 gcggctcctt gcgacggaga ccggtgtcga ctcacatcgg ctccggctgg gcatgatcga
     3121 cgacacagaa tgggatcgga tctcgcgtgc atttggcagg ctctccgaag ccaacatctt
     3181 catcgacgat tccgcgaacg ccagcatcat ggatgttcgt tccaaggcgc gccgcctgca
     3241 ggctgaaaaa gggctggacc tcctgatcat cgactacctg cagctcatgt ctggtcgccg
     3301 ttccgaaaac cgcgttcagg aaatttcgga aatttcccga ggtctcaagg ggctcgcgcg
     3361 cgaactgaac atccctgtcg tagccctctc gcagctatcc cgtgccgtag aaacacgttc
     3421 cgaccaccgg ccgatgctct ccgatctccg cgaatccgga tcgatcgagc aggacgctga
     3481 tatcgtgatg ttcatttatc gggaggaaaa gtatgacgag aacagcgaca agaagggcat
     3541 tgcggagttg atcgttgcca aacaccggaa tggtcccgta ggatcgatca acctgcgctt
     3601 cttcgagcag actgcgagat tcgctgatct cgagctctac cgcgagccgg atgcctcatg
     3661 acccggtctt cgtcttccct taccgccatt acttcttccc ggctcccggt cagcattccg
     3721 catgcattcc tgacagagac ggtcatcaac atcacctgca tggcggagct ccaggtcagc
     3781 ctcgcgatgt tccggctggt tgccgttctg gagggcgagg aaacgccgat tgcagaacgg
     3841 atgatcctga gggatgaagc cctcaaccgg gcgatgcggc gtgacggcat cgtacctgag
     3901 cctgccgaac gtatcctcaa gggattggag cttgccgtag gtcggggaac catcctgcgt
     3961 tttcttgcca cgcagccgag ccatcaagct tactggtact acctggacac cacagtcaac
     4021 cgcgcgaccg tcgcatcaat ggagcgcgga gcgttgccac ctcccgctat catttgggaa
     4081 ggcaaaacag cgccagcgat ctccctcgac ccgcctaacg cctatcgact ctatgagcaa
     4141 aacatcggac cgctcactcc gctgatagca gaccaaatca cgcgggccgt tacggattat
     4201 cccggcgact ggatcgagga cgctatcacc gaggcagtga gctacaaccg tcgcagttgg
     4261 cgttatatcc tgcgaatcct tgaaaattgg caagcttccg gccgtcagga gcaggaacac
     4321 tacagcggca atgcttgatt ttcaacgacc ctgccggatg tatagaggaa tagatcatga
     4381 aacccattgg tgacgttatc aaaaacatcc cggtgtcgag gtccggcaaa agtcccaacg
     4441 gcagcgccat tcgcattacg aggaaggatg acgtccgatg cccgatctgc caggacgcgg
     4501 ggtatctccg cgcggacgtg cctcccggtc acccaagctt cggtcgactc tttccctgcc
     4561 aatgtaagag aaacgagctt gatgagcgac gggcggaaga tcttcgccgc ctttccaacc
     4621 ttgacgcttt taccgggtat acgtttgacg atttcgaccc ctcgacatcc ggtacagagg
     4681 aagccttcga ggccgccctt gcttttgcac aggagcccga tcaccgctgg ctcttccttt
     4741 caggtccctg tggcgttggg aagactcatc ttgccgttgc gatcgcgacc tatgcgatgc
     4801 agtggcacca catgagcgtc tatttcgccg ttgtccctga tctgctggat cacctccggg
     4861 caacgttcga ccccaactcc ggctcggcat atgatgagcg cttcacctct attcgtaacg
     4921 ctccactcct cgtgctggac gatcttggca cggagaatgc cactccttgg gcacgggaga
     4981 agctgtacca aatcatcaat caccgttata gcgagcagat gccaacggtt atcacatcca
     5041 atgtggaact cagaaaggtg gatgaacgca tcgtttcacg gatgttggat catcgtctca
     5101 cccaatacgt ccagatcgac gccgaggatt tcaggagacc cggcgatgcg aggcgggttc
     5161 gccggacacc acggcgctag tgcaaggggc tttcttccga tcctttgaac cgcggacctg
     5221 tgaggaatgc atgctggcga ggaagaactg ctgtcagcga ttatcagtgt agaatttccg
     5281 gtgtcaactc tccccggatc gaacgagtaa agaggctact gttccggtaa ggtcggacga
     5341 catgttgcac cgttcgaccg ggttgtcgcc actgcgagtt acaggagatt cgtcatttcc
     5401 ccccgtcagc ttcgacggcg tagcgtcgag atcatcgaaa tatggcaaat tcgatgagcg
     5461 acctcacatg gttgtttgag cggctgttcg acgtacgagc gatcctcgat atcctggttg
     5521 tagcgttcct catctattgg ctcttgtggg tggcacaggg aactcgcgcc ctgagcctta
     5581 tccgcggcat catcacgctc ttcctcttcc tctatttgct cggcgatggc ctccagttaa
     5641 cgaccctcaa ccggatactc tcggtcatct ggccggcact tattatcgcg atccccgtca
     5701 tttttcagcc ggagttgcgg cgcgcattcg agcagttggg ccatgcagga tcctggcttc
     5761 gaagtccatt tcctgcccag gatgatcagg ccgcggagtc gatgatcgac gagatcgtga
     5821 gagcgtccca ccaactctcg cgcttgcgtt acggtgcact catcgtgatc gagcgtgaga
     5881 caggcctaca ggacttcgtc agtaagggcg ttcaactcaa cgccgacatc tcacgtcagt
     5941 tgctgatcaa catcttcttt cccaactctc cccttcatga cgcagcagtc gttatcgggg
     6001 ataaccgcat tttggctgcg agtgtcgtcc tgcctctgac ggataatata tccgcgtcga
     6061 gtcagctcgg gacccggcac cgtgccgcaa tcggcatcac cgaggaatct gactcactgg
     6121 cggtggtagt ctctgaggag acggggcaaa tgtcggtggc gcactcgggg cggttgatcc
     6181 gaaatctcga ccaggatcgg ctccggcggg tgctgaggac gcttatgcgt gtcgacaggc
     6241 cagtgcagaa cggacggctc gatggccgtt tcggcagatt gagggctctc attcccgcag
     6301 cttcagactc ccgactcgcg gacggttccg caggaggtac tcggcgcgaa cgacgacaag
     6361 aatcgataca ccaaccgtga gagaccacgc accttcgccc gcatccccca ttggggccct
     6421 cgcgtttcga ccgacccggg tccaagttgt ccggttcgta ttttcgctgc tgattgccac
     6481 catcctctgg ggctgggtga ccgagctgaa cgatccattc atcacggtta ggtaccgtga
     6541 gctagacgtg actgcggggc cattggacaa ctccctgcgg gtcgtaacaa ctcttcctcg
     6601 tgccaccgtc acactgcggg gacctgaatc cagagtcgaa gacatctccc gtgacgaggt
     6661 ctccgtggtg ctggacacga gcgaggtcac cgaggcagga gaatatcgcc tggaactgga
     6721 tgttgtagcc ccggagggcc cgagttcgaa gtcagtaaat ccggaagagc tccccgtgac
     6781 gatcgagcag gaaatcagcg aggtcatgcc gctggaagtt cgaaacgcag tcccacgaga
     6841 cgacccccgt gaggttacat cgaccgtgcc tgagacgaca caagtgacgg tctctggccc
     6901 ctcatcagcc gtgggccggg ttgaggaagt gatccttcct gtctcgctcg ataatcatca
     6961 gtctagcttt gaggaagcct tcaccccgta cgccgtggat tcggaagaac agcgagtgag
     7021 cgaagtccgg atattaccag aagaaatcat gacgagcgtc gatatccgct ctcgaggtaa
     7081 gacagtatcc gtcatcccag tgatcgaagg agttcccgaa gagggtttct ccgttcagca
     7141 gcgcgccgcg ctgcccgact cgattgttgt cgaaggtccg gacgaggcgc tggaatcact
     7201 cctattcgtc aacactgagc cagtcagcat cgcaggagca acgcagtcga tcagcagtga
     7261 ggtcgggctt tcgaacctgc cacccggcgt gaccgtcatc gagccagaga gcggcaaggt
     7321 agaagtacgt gtggcaatag aggacaccac gagcaccgcc cagacgctca ccggctcgcg
     7381 cgttgaaccc atcaatctgg agcccggtct ctccgccgtc atcgaacctg agggcattga
     7441 cgtgacagtc gacggaccgg gtagcacgct gtcaaagatg acgccgtcgg atgtaaagac
     7501 tcgggtagac gtgtcgggtc tcaggcccgg aacatacgag gtttcccctg aaatcactgt
     7561 tcctcaaggg gttacgtggg tcggtaatta tccccagacg gtccaggtga caatcttcgc
     7621 agttcaggag acaccagggg cttctccgat tccgcaggct tcaccgggca cctagcgact
     7681 agcgtgttgt ggccctttcc tcaccatgaa acccctccat gggcatccgc ccggtgtccc
     7741 atcgagtggt gaacgctccc tgagcaccgc aacgaaagag agagccgggg tgaaagatca
     7801 tcccggctct ctcgttggcc acatgcaaga aaaagatcac tcgttgttac tggaagtatc
     7861 accgtcgaag gtatcaccgg caccagacgc gatcgagaaa tccatttcgt gagccaagag
     7921 gatcgcctcc gcgacctccc tcatcgactt ccgggcgtcc atgctcgtct tgcggatgcg
     7981 atggaatgct tccgactccc gcaatccatg ggtttccatg agaacgcctt tggcccgctc
     8041 gacgattttc ctcgtctcca gcgcatcctt gagcccgccg acttcccgtt cgagcgtccg
     8101 aaactcctcg aatcgcgctc gggaaagctc gatgacaggc atcagttctg cctcccggaa
     8161 gggcttcacg acgtagccca cgacgccggc ctctcgggcg cgttccacga gttcctgatc
     8221 cgaatatgct gtcaagagca cgaccggagc tatccgctcc ctcgtcaact cctccgccgc
     8281 cgagataccg tccagatcgg gcatcttgat gtccataacg aggaggtccg gctgtaatcg
     8341 cttcgccagt tcaactgccg tgcggccgtc acctgcttca ccgatcacct cataaccaag
     8401 gtgcgtgagc atctcccgca agtcaagccg gatgagtgat tcgtcatcgg caatgatgat
     8461 acgccagtgt gttgagactt gaggagcgcg gctcattctt ggcaaacctc acggcgtgga
     8521 tgatagcgaa accgccaacc ggacagggag ccaatacgct ggtgaggaat gcggtggcga
     8581 gacaaaggag tcatcgatgg atcgtcatcg ttcgggggaa caggtgctgc aagccggcaa
     8641 ggagatcctg cctgcctcgt tttcatgcaa ggtccatgac aacccgttcc cgtgtggcgg
     8701 tgttccgaat aatactctgg tcggggcgag aggattcgaa cctccgacca ccggtacccc
     8761 ataccggtgc gctaccggac tgcgccacgc cccgaaaaga acaccgccgc tgcaaaagta
     8821 taagtcagga ccccgctccg tacaattaca tgtaggagcg gggatgtcca ttccggttct
     8881 ggaaggactt tccctcccgg agggagcctg cttttacttg acgtcgacct tggctccgac
     8941 ttcttcgagc ttcgctcggg ctgcctcggc ctcgtccttc gtgacaccct cgcggaccgc
     9001 cttgggtgcg gaatcaacga gatccttggc ctccttgaga ccgaggcttg tcagctcgcg
     9061 gacggccttg atgacctgga tcttattggc gccgatatcc gtcagcacca cgtcgaactc
     9121 ggtcttctcc tcttcttcgg cggcgctagc accaccggca gctgcaccag cagcgcccgc
     9181 aacggcaacc ggagcagcgg cggatacgcc ccacttctct tcgagcgcct tcacgagctc
     9241 gttgagctca aggacggtaa gaccttcaag agtgtcaata agctgagcct gatcggccat
     9301 gtttttgttc tccatcgggc ggacctcatg cccgcctagt gttgtgaaag gtgtcaaata
     9361 caaacaaaca gcgaaacgct ttaatcagcc gcctgcgctt cagtggcgcc acccagctgc
     9421 tcgatccgtg cgttagcaag ataggcaacc gagcgtgccg gtccactgag gacgcccacc
     9481 gtacgggcca tcggcgatac cagcattccg acaagcttgc cgagcagttc ctcgcgtggc
     9541 ggaagggtgg agatcgcctc gatatccgag gggcctacaa cctggccgtt catgacgccg
     9601 accttgacct gcaaaatacg tgaggtccgg gcgaaatctg aaacggcctt cgccggtgcg
     9661 acgatatcgc cgaaggcgaa cagcaccgcg gtcgggccat cgagatggct ctccagcccc
     9721 tcgataccga cgcgctccgc tgcgatccgg accaaggtat tcttggcgac ccggaactcg
     9781 gactcggtcg agcgaagtgt ggcgcgaaag ccctgcaact gacttaccgt gagaccacgg
     9841 taatccgcaa cgatcgtgag ctgcgatcgc tcaagccgat cgaccaacag gtcaatttct
     9901 gctccctttt taagagttgg catttgtttc acctccttcc tgtcatggaa tatttcgaga
     9961 ggtcttgccc ctctccctgg caaagaaagc gccctaggca gaaacagccc agggcgcaga
    10021 ccatgtgtgg tcggtgcgtt tcctgagcct cggtcgggga ttaagtgagt ctcctcacac
    10081 cgactgtctt cggccagtac acagtccttg ttgggttaga aagaattaac tcgcgacgtt
    10141 ggatgacttg gacagcgtgt cgttgacatc aagccggata cccgggctca tcgacgtcgc
    10201 gatcgcaagt gtacggcaat agataccctt caggccactc ggcttgtttc ggttgagggc
    10261 gtcgactact gccaggatgt ttgcctggat ttgatcttcg ctaaagctct tgcggccgac
    10321 cgtcacgtga acattgcccg tccggtcgtt tcggaattcg acacggccgc ctttgagctc
    10381 ccgaacgacg ccggagagat cagcgacctc gcggaccaca gtaccggagc gtgggttcgg
    10441 catgagccct cggcggccaa ggatccggcc gagtcctccg accttgccca tctggtctgc
    10501 cattgcgatc gtggcgtcga actcgagcca gccgccctga attcgctgca ccagttcgtc
    10561 ggatcctgcg tggtctgctc ccgcctcaag agcggcacgt tctgcctcac cggcagcgaa
    10621 cacgagaaca cgaacctggc gtcccgaccc gtgcgggaga gtcacagtgg accgaacagc
    10681 ctggtcagcc tggcggggat ctatgccaag ccgcgcatgc aattcgacgg actcgtcgaa
    10741 gttcgcgaat gcaaccctct ttagcagctc ggatgcttcc tcgactgtgt actgttgttc
    10801 ctcatccacg agcttcgcag cctcgagata cttcttaccg tgtcccatgt ttacctcccg
    10861 gggttcaagc ggggctgtgc ccctccccca tcattctgcc tgttcgccct caattagctg
    10921 gcgacttcga tacccatact gcgagcggtg ccttcgacct gcttgattgc accctcgatg
    10981 tccaccgcgt tcagatcctt gagcttgagc tctgcaatct cccggacctg atcctggctc
    11041 acgctgccga ccttggtcgt ctttggattg ccagcaccct tctggatacc ggccgccttc
    11101 cgaagaagat ccgccgccgg cggtgtcttc gtcacaaagg tgaaggatcg gtcctcgtaa
    11161 acggtgatga cgaccggaat gatctggccc gccatcgcct gtgttcgctc gttgtactcc
    11221 ttacagaagt tcatcagggc gataccatgc tgacccagag ctgggcccac cggaggtgcc
    11281 ggcgtcgcct ttcctgcagg cagctgcagc ttcacatatc cacgaatttt cttagccaac
    11341 gaacgttcct ttctggctgt cgtacacgtc tcgccgtcgc actagttgca cggcgagtct
    11401 gattctccca atacagacta agtcaaacct cgcgttccgc ctgaaggaaa tcgagttcca
    11461 ccggcgtctc gcgaccgaag aaggagacca gaactttgat cttgcccttc tcgttattga
    11521 tctcatcgat ctcgccccga aagtcggcga acggaccatc gataatccga accgtatccc
    11581 cgagagcaaa ggacattgta acctttggtg cttcggcttc catgccttgg atgacaacct
    11641 gcgcagcgtc acccttcagt ggcactggct tgttgtcctg gctcacgaac ccggtgacac
    11701 ccggcgtgtt ccggagctgg taccacgtat cctcgtccat ctccattcgg acaaggacgt
    11761 atccaggaaa gaccttgcgc tggaccgtct tacgttgacc gtccttgatc tcaatctcat
    11821 cctgagtggg aaccaccacc tcgtagaccc ggtcgccgag gtcgaacgcc tcgacgcggc
    11881 tctcgatggt cttcatcacc ttgttctcgt acccggaata ggtgtgaaga acgtaccact
    11941 ggccctgggt tatcgagccg tcgtcgcccg cttcttctgc ccgcttggct gcgagacgct
    12001 cacggagcgt ggggcgttcc attcggaacc tccttcaatc ttcttgttgg tctctcatcc
    12061 taggttagag cctcgaaaag ctggaacaac acgtagtcga tccctcccag cagcaccccc
    12121 aggacaaacg acagcgcaat cacgacaatg accaggttgc gagtcgtttc tcgatccggc
    12181 cattgcacct ttcggatctc ggatgcggca tcttcataga agcgctgcaa gccaccgaac
    12241 cgttccgtgt tcacgaattg ccggcggccc tccttcacag gctttcggct cttcgcctcc
    12301 cgccgctcct ctttccgccg caggtgcttc tctttctgaa gctcgctggc gtctccaggc
    12361 tcgacggctt cccgtgcgga ggctttccga tccgctcgcg attcctttcg cgattcttca
    12421 accggcttta agcgtaccga ttcctcggtc accatggagg agggtgcgtg ctcaagctgg
    12481 tcgaccgacg acgcctgtga cgccggagcc tgcccttgac ggagatcggc ctggcgcttg
    12541 cgacgccgat tcgccgcaga ggatcttctc tttggagcgg tagtatcttg cgtgctcaaa
    12601 tctcggatcc ttcacaaaaa gaacgcggtt tggttcccag gcggaatcgt cgaactgaaa
    12661 cggcgggaac tttccgcatt atatcggcaa atcttgcaca gaaatggggc gtgccggctg
    12721 gctccgcccc acctccactc ggattatcac cgggcagggc cggcaggaat cgaacccgca
    12781 acctgcggtt ttggagaccg ctgctctacc taattgagct acgaccctac gaccacccta
    12841 agagcggtgt tatcgcgtct cgcgatgaag ctggtggcag cggcaacgcg gacagaactt
    12901 cttgagctca agccgacccg gatcgttacg acgattcttc tcggtggtgt aatttcgctc
    12961 acggcaaacc gtgcactcca tcgtaaccac cggccggtca accttcgact tcttggccat
    13021 tgtactacat ccttatctgc gtctcacaac gcccgcaaca tattgaccga acaattatca
    13081 gcgtatggcg ccgccggtat caaccaggca ccgccaaaac gaaattgtgc tctactccgt
    13141 gatggaggtg acagccccgg cgccgacggt ccggccgccc tcgcgaatcg caaagcgcag
    13201 cccctcctcg atcgccaccg gcgtgatcag ctccaccccc atctggatgt tgtcgccggg
    13261 catcaccatc tcc
//
LOCUS       DASYWM010000205         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_102466, whole genome shotgun sequence.
ACCESSION   DASYWM010000205 DASYWM010000000
VERSION     DASYWM010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102466"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VGR29_08380"
     CDS             <1..724
                     /locus_tag="VGR29_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV2073640.1"
                     /translation="VKIDPEAVPATVPFGSSAELQPGQDVLAIGSPLGAFSNSVTAGI
                     VSALGRNQLSSDNATCQNYSNLIQHDAAINPGNSGGPLFNLQGEVVGVNTLGIPIDES
                     GQPVQGLFFAVPSSTVKTAVQQLIETGTVTQAWVGIAFHTIDPSFANQYDLPVDHGIL
                     VTDVEAGSPADQADLQPGDIITAIEGTEITPTRALADIMFQHRPGETVTLSIIRDGEQ
                     SEVELTLGETPLNFEDCTLPGE"
     gene            975..1727
                     /locus_tag="VGR29_08385"
     CDS             975..1727
                     /locus_tag="VGR29_08385"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV2073641.1"
                     /translation="MNQPNVYRDYFRRLYALFEEVLDSQASRIESVAAWMADSIAAEG
                     IVHLFGSGHSHMVAEEVFHRAGSLMPLNPILDPNLTLFGMVNATLLERTSGYARVVIG
                     THDIRNGEVVIVISNSGVNPVPIEAVLEAKARGARTVAMTSDRHYASAPSRHDSGQRL
                     ADIADITIDTCVPRGDALVEIPGVEFSVGGASSVVGTALINAIVVETTHRLHMMGVTP
                     PVIPTMNLPGGDERMHELVRAYDKRLPLLRNA"
     gene            1916..>2626
                     /locus_tag="VGR29_08390"
     CDS             1916..>2626
                     /locus_tag="VGR29_08390"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEV2073642.1"
                     /translation="MDRDMNQFIPQNGMTVVGADGEKVGDIDGVEQDYFIVRKGFFFP
                     QDHYIPMTAISSYDDSQVYLNVTKDEALEQEWSNPPAAGTTYAETTTVGDQATIVDDR
                     DAYGDTDLLTDRDGVVVEDSGRVGAVGEPDVATYDDDRDTLAGVETGVDRGVDVGVTR
                     GVDDSVIEVHEEELGAATREVERGEVRIDKNVVQEQQTLEVPVTEEEVEVTRRRVDRP
                     VTADDHAFEEGTIEVPLRG"
BASE COUNT          551 a          792 c          777 g          506 t
ORIGIN      
        1 ggtaaaaatc gacccggaag ctgtccccgc aacggtccca tttggaagct ccgctgaact
       61 ccagcccggg caggatgtat tggccatcgg ttcaccgctt ggagccttct ccaactcggt
      121 gaccgctggc attgtgagtg cgctcggccg caaccagcta tccagtgata acgccacctg
      181 ccagaactac agcaacctga tccagcatga cgcagcgatc aaccctggca attccggggg
      241 accgctgttc aacctgcaag gggaagtcgt cggcgtcaat acactcggta tccccatcga
      301 tgagagcggc cagccggtcc aggggctttt cttcgccgtg ccaagcagca cggtgaagac
      361 ggcagtccag caacttatcg agacgggcac cgtcacgcag gcatgggtag gcatcgcttt
      421 ccacacaatt gatccgtcgt tcgcgaacca gtatgacctg ccggtcgatc acggcatcct
      481 cgtcactgac gtcgaagccg ggagcccggc cgaccaggcc gatctgcagc ctggagacat
      541 tatcaccgcc atcgaaggca cagagattac cccgacccgg gcactggcgg atatcatgtt
      601 ccagcatcga ccaggcgaga ccgtcacact aagcatcatt cgggatggtg agcaaagcga
      661 agtggaactt accctcggtg aaacaccgct caacttcgaa gattgcacct tgccaggcga
      721 gtagcagccg caggctcgtc tctccccaat gtcaccttga ggcctggagc accgagcgcc
      781 ctgtcggggg cgttcagtgt ttcaaaactg aatcacttct cctccatgca gcgccaggag
      841 ctgcatcttc cataatatct gcagggtgcc acggtattcg tcgctgtcgg ccacccacca
      901 acagaaacat cagcccgatc ttcactgcga cccgcatggt tccgcagcaa ttggcgagta
      961 cgcacgagga gccaatgaac cagccgaatg tctaccgcga ctatttccgc cgcctctatg
     1021 ctctcttcga agaggtgctg gattcccagg cgtccaggat cgagagcgta gctgcatgga
     1081 tggctgactc catcgccgcc gaaggcattg tccatctctt tggtagtggg cactcgcaca
     1141 tggttgcgga agaggtcttt caccgggcgg gcagcttgat gccgctcaat ccgatactgg
     1201 acccgaacct gaccctattc gggatggtca atgcgacact gctggagcgc acttccggct
     1261 atgcccgggt cgtgatcggc acccacgaca tccgcaacgg cgaggtggtg atcgtgatca
     1321 gcaattccgg ggttaacccg gtcccgatcg aggcagtgct cgaagcgaag gcccgcggcg
     1381 cccgcacggt ggccatgacc tccgacaggc actacgcgag tgctccatcc cggcatgatt
     1441 ccggtcagcg cctcgccgat atcgcagata tcacgatcga cacctgcgtt ccgcgtggcg
     1501 acgctttggt ggagatcccc ggagttgagt tttccgtcgg cggtgcctca tccgtcgtcg
     1561 gcacagctct catcaacgcc atcgtggtcg agacgacgca tcggctccac atgatgggcg
     1621 tcacaccgcc agtgattccg acaatgaatc tgccaggcgg ggatgagcgt atgcacgagc
     1681 ttgtccgggc ttacgataag cgtctcccgc tgctccgaaa cgcctgatcg ttggtcccgg
     1741 ccggtcctct cgccgcgttc tggcgcaccc aggatgtggt catcaacaag tgtggcacgc
     1801 cccgtgcgag ccttgtcaac aggagagtga cgcggcggta atgactgccg aaacgtttct
     1861 cgacatcgag gtggatgcac aaacacaagc atcagtatct tcaggaggta caacaatgga
     1921 ccgtgacatg aatcagttca tcccacagaa cggcatgaca gtcgtcggtg ctgacggcga
     1981 aaaagtgggt gatattgacg gcgttgagca ggattacttc atcgtgcgga agggtttctt
     2041 cttcccgcag gatcactaca tcccgatgac ggccatctcg tcctacgatg acagccaggt
     2101 ttacctgaat gtcaccaagg atgaagcgct tgagcaggag tggagcaatc cgccggctgc
     2161 cggcacgacc tatgcggaga ccacgaccgt gggcgaccag gcgacgatcg ttgatgaccg
     2221 cgatgcctat ggcgataccg atctccttac ggacagggac ggtgttgttg tagaggattc
     2281 cggtcgcgtt ggtgcggtcg gcgagccgga tgtcgcgacc tacgatgatg accgcgatac
     2341 cctcgcgggc gtcgagaccg gtgtggaccg cggcgtcgac gtaggcgtca cccgtggcgt
     2401 tgatgactct gtcattgagg tgcacgagga agagcttggt gcggccacac gagaggtcga
     2461 gcgcggcgag gtccgtatcg acaaaaatgt ggtccaggag cagcagacgc tcgaggttcc
     2521 ggtgacggaa gaagaagtcg aagttacccg acgtcgtgtc gatcgtcctg taaccgccga
     2581 cgaccacgca ttcgaagagg gtaccatcga ggttccgctt cgtggt
//
LOCUS       DASYWM010000206         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_196485, whole genome shotgun sequence.
ACCESSION   DASYWM010000206 DASYWM010000000
VERSION     DASYWM010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196485"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VGR29_08395"
     CDS             complement(<1..489)
                     /locus_tag="VGR29_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2073643.1"
                     /translation="MSRTFPMYLAGYWKENKHTVEVRNPFDDSVVGTTFLAQPKDLEL
                     ALTSALGIFPEMKAAPTYTRAQWLHDLSAAMAEERNTLIELIAMECGKPVKEATTEVD
                     RGIFTVENAAEEAKRIEGEVIPLDLLPSSKSRLGIVRRFPIGPIAGISPFNFPLNLAL
                     HKA"
     gene            complement(583..1089)
                     /locus_tag="VGR29_08400"
     CDS             complement(583..1089)
                     /locus_tag="VGR29_08400"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEV2073644.1"
                     /translation="MLDEVKGFSVTLKRLFAPKVTQQYPDEKRAMMPRYRGLPALRSD
                     PDTGEALCVACGLCARICPTSCLDMNVVPSEEGDRELGEFILKSGRCLFCGFCAQVCP
                     VDAITMSQEYELTVMERDGLVYTKTELATIGGQSPIWWESGVGPENQQTNAPSYYDPF
                     RDKGAELY"
     gene            complement(1107..>2752)
                     /locus_tag="VGR29_08405"
     CDS             complement(1107..>2752)
                     /locus_tag="VGR29_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2073645.1"
                     /translation="RWGYEKIQSTDRIFYPRSRDADGRTYEMGWDEAVDLVAEGLAHY
                     QGETFGALASGENTNEEAYVLQQFSRAVMQSNNVNRLMTQNHAAVERVTRAALGDDVA
                     HTNNMQELFSAVHNLLVVGPSIIDYAPIASYWINHSRLYRETRIVVVATEHYPLCDRA
                     AVWLQPNPNTTSLVLEAVAAEVIRLGLGGSELGLKPDVDLEEVAAKSGVPAAMIREAA
                     IVFVTGQHEMPQERPEAGFPPAAIFHTVAHQTPIDHVVDYRIEDRSVDTDYSDAGRIA
                     AACINLAILTGNIGRVGGGVASPRGPANHQGTTDMGLVPDLLPGGLRVTDPAAREKLE
                     ALWLGRWADKASTSNGFVPVKRLPTEPGVAYEELADAIKSGRIKAMWVEGGQWTRQIV
                     ANQPLYEALQKLELLIVVDGYDSPLAQIAHVVLPLALNLEKDGTFTNFDRTVQRVRAA
                     VPPMGEARSEIDILSAVSRRMGYGLQADHPSRIMAEISRVVPWYASVTYARLERGGIV
                     TPVTSLGEQGATILTSNSDSPRPLQPRLISMSSAPVSAD"
BASE COUNT          530 a          839 c          841 g          542 t
ORIGIN      
        1 ggccttatgg agggcaaggt tgagcggaaa gttgaacggt gaaatgccgg cgatgggccc
       61 gatgggaaag cggcgcacaa tgcctaatcg gctctttgaa gacggcagca ggtcgagcgg
      121 gatgacctcg ccctcgatcc gttttgcctc ctcggccgca ttctcgacgg tgaagattcc
      181 tcgatcgacc tcggttgtcg cttccttgac cggcttgccg cattccatgg cgatgagctc
      241 aatcagggtg ttccgttcct ccgccatggc cgcggagagg tcgtgcagcc actgtgccct
      301 tgtgtacgta ggagcggctt tcatctcggg gaagatccca agcgcggacg tcagagcgag
      361 ctcgaggtcc tttggctgcg ccaggaaggt tgtccccaca acactgtcgt cgaaggggtt
      421 cctgacttcg accgtgtgct tgttctcttt ccagtaaccg gccaggtaca tgggaaatgt
      481 gcgactcaag gttgctacct cctggaaatg tgatgcccga ccagaagatg gtctcctctg
      541 ccgggcatcg attccttgct ttcattcaag ctttgtggtg ccttagtaaa gctcggcgcc
      601 cttgtcccgg aacgggtcgt aatacgatgg cgcgttcgtc tgctggttct ccggcccgac
      661 gcccgattcc caccagatgg gggactgtcc accgatggtg gccaactccg tcttggtata
      721 caccagcccg tcgcgctcca tcacggtaag ctcgtattcc tggctcatcg tgatcgcgtc
      781 gaccgggcag acctgggcgc agaagccaca gaagaggcac cgaccggact tgagaatgaa
      841 ctcgcccagc tcgcggtctc cctcctcaga ggggacgacg ttcatatcga ggcaggaggt
      901 ggggcagatg cgcgcacaaa gcccacaggc gacacataga gcctctcccg tatccggatc
      961 ggaccggaga gccgggagcc cgcggtaccg aggcatcatg gctcgcttct catccgggta
     1021 ctgctgcgtg accttcggcg cgaagagtcg tttgagcgtc actgagaagc ccttgacttc
     1081 gtccagcatg tgttgaggtc cctttcctaa tcggctgaca ctggcgcaga actcatggaa
     1141 atgagtcgtg gctgcagcgg ccggggtgaa tcggagttcg aagtcaggat cgtggcgccc
     1201 tgctcaccca gggaagtcac cggggtgaca atgccgccgc gctccagacg ggcgtaggtg
     1261 acacttgcat accacgggac gacccgggag atctccgcca taatccggct tgggtggtcg
     1321 gcctggagcc cgtaacccat gcgtcgtgat acggcgctca ggatatcgat ctcggaacga
     1381 gcctcaccca tcggcgggac agcggcacgg acgcgctgga ccgtgcgatc gaagttggtg
     1441 aacgtgccat ccttctcaag attgagggcc agtggcaaga caacatgcgc gatctgggca
     1501 agaggcgaat cgtagccatc gacgacgata agcagttcca gcttctgaag cgcctcgtag
     1561 agcggctggt tcgctacaat ctggcgcgtc cattggccgc cctcaaccca catcgccttg
     1621 atgcggccac tcttgatagc atctgcaagt tcttcgtagg cgacaccggg ttccgttgga
     1681 agccgtttga ccgggacgaa gccgttcgat gtcgatgcct tgtcggccca acgtccgagc
     1741 cagagcgcct cgagcttttc acgcgcagcc gggtcagtca cccgcagacc accgggaagc
     1801 agatccggga caagccccat atccgtcgtc ccttgatgat ttgccggacc cctgggcgag
     1861 gcaactccgc cgccaacccg accaatgttg ccggtgagga tggcaaggtt gatacaggcg
     1921 gcggcgatgc ggccggcgtc gctgtaatcg gtgtcaaccg agcgatcctc aatcctgtag
     1981 tcaacaacat ggtcgatcgg cgtctggtgg gcaaccgtgt ggaagatcgc cgcgggcggg
     2041 aatcccgctt ccggccgctc ctgcggcatc tcatgctggc ctgtcacgaa gacaatcgcc
     2101 gcctcgcgga tcatagctgc cggcacaccg gacttcgccg caacctcttc gagatcgaca
     2161 tccggcttca gacccaattc gctgccgccc aaaccgaggc ggatcacttc agctgccacc
     2221 gcctcaagga caagtgacgt ggtgttcggg tttggttgga gccacacggc ggcgcggtcg
     2281 cagaggggat agtgctccgt agcaaccacg acgatccgcg tctcacggta gaggcgggag
     2341 tgattgatcc agtaggacgc aatcggggcg tagtcaatga tcgaaggccc gaccaccagg
     2401 aggttatgca ccgcggagaa gagctcctgc atgttgttcg tgtgtgcaac atcgtcgccc
     2461 aatgccgccc gtgtaacacg ctcaacagcc gcatgattct gggtcatcag gcgattgacg
     2521 ttgttcgact gcataacggc acgagagaac tgctggagaa catacgcttc ctcattggtg
     2581 ttctcgccag aggccagagc gccgaacgtc tcgccctggt aatgggccag tccctcggcg
     2641 acgaggtcaa ccgcctcgtc ccagcccatc tcatacgtcc ggccatcggc atcgcgcgag
     2701 cgcgggtaaa agatcctgtc ggtggactgg atcttctcgt atccccatcg cc
//
LOCUS       DASYWM010000207         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_496227, whole genome shotgun sequence.
ACCESSION   DASYWM010000207 DASYWM010000000
VERSION     DASYWM010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496227"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VGR29_08410"
     CDS             complement(<1..1155)
                     /locus_tag="VGR29_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV2073646.1"
                     /translation="MIALWLLLVGLVLPFVPNIDGPLKVGGFSSDESEGARATQILEE
                     ELGFSPSQLAIIFTSDTLKANDSSFLDQVDASLNEVKRLDFVDDVITPSLDPTLIASS
                     GRLAYAVVGLGVPVEEAQAEARAVQEALIEQPDLDVLVAGGPSFYADVEVASQRDLQR
                     AELIAFPVALIALIIVFGSLVAAAVPLLVGSAGVAVALLGLYWTAHIVDMSIFVLNLT
                     TMLGLGLAVDYSLFVTSRYREELARRGGDVDESVVTAMSTAGRAVFFSGLSVLVGLSG
                     LSLFNLMFLRSVGIAGVIVVAISTSAALTLLPAILKIIGLRINALPMGRLARRHATEQ
                     VTTGFWYRLAKRVEQHPGLVTAGVLVILVLLGSPFLRANISSPDATMLPED"
     gene            complement(1231..1653)
                     /locus_tag="VGR29_08415"
     CDS             complement(1231..1653)
                     /locus_tag="VGR29_08415"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEV2073647.1"
                     /translation="MSRLSAPMGIRSVRERSLRYAGSTECREKGVTYHLVPSEVWDRQ
                     SDAPTYLPEGYDREGFIHCTNGLDELVAVGNRYYKADERQFRVLILDLSKVESPVRYD
                     DPDEVFPHIYGPLNTNSVVGLLLARRQEDGTFVSYTRE"
     gene            1751..>2532
                     /locus_tag="VGR29_08420"
     CDS             1751..>2532
                     /locus_tag="VGR29_08420"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV2073648.1"
                     /translation="MRIGIIINPGSGRRSASRHRSMVEQIGADVAADLSLVVYDGSER
                     IESLVSRMVSTIDILGIVGGDGTLNGAINGVMRSENPEVPVFFLPAGRGGDTARTLPA
                     LPPNYANVRSFSYRTCKIDLGLLSTQGGESRYFINESSIGLGAFAARSASRLPRKIGS
                     TAYLIGTIAGLFRSPTTDLRLVIDGTGTVELPGCHHLTVANGRYFGAGLQIAPSASAV
                     DGLLDIIAVADAGAFGIARALPRLLRSTHLSHPIVRHWKSTGV"
BASE COUNT          557 a          774 c          710 g          491 t
ORIGIN      
        1 gtcctcgggc aacatcgtgg cgtccggcga cgagatgttt gccctgagga agggagaccc
       61 caacaggacc aaaatcacta gcacaccggc cgtgacgaga cccgggtgct gctccacccg
      121 tttcgcaagt cggtaccaga agccggtcgt cacctgttcc gttgcatgcc ttcgcgcaag
      181 tcgtcccatc gggagagcgt tgatgcgaag accgatgatc ttcaggatgg ccgggagcag
      241 cgtgagagcc gccgaggtcg agattgcgac cacgatcacg ccggcgatgc ccaccgacct
      301 caagaacatg agattgaaca acgataatcc ggataggccg acgaggacac tgagtccgga
      361 aaagaaaacg gcgcgccccg cggtggacat cgccgtcacg acggactcat caacatcgcc
      421 gccgcggcgc gccaattctt cccggtaccg gctcgtgaca aagaggctgt agtcgacagc
      481 gagtcccagg cccagcatcg tcgtcaggtt gagaacaaag atgctcatgt cgacgatgtg
      541 ggctgtccag tacagtccga gcaacgcgac agcgaccccg gcactgccca cgagcaacgg
      601 tacagccgcc gcaaccagcg acccgaagac gatgatcaat gcaataaggg caaccggaaa
      661 cgcgatcaac tctgcccgct gcaggtcccg ttggctggca acctccacat cggcatagaa
      721 cgacggtcct ccggccacga ggacatccag atcgggttgc tcgatcaacg cctcttgcac
      781 cgcccgtgct tccgcctggg cctcctccac tggcacgccc aggcctacca ccgcatatgc
      841 aagtcggccg cttgaagcaa tgagcgtggg atcgagtgat ggggtgatca cgtcatcgac
      901 gaagtccagg cgtttgacct cattcagtga agcatcgact tggtcgagga agctggagtc
      961 gttggccttg agcgtgtcac tcgtgaagat gattgcaagc tgtgaggggc tgaaacccaa
     1021 ctcctcctcg agaatttgcg ttgcccttgc gccctccgat tcgtcagagg agaatcctcc
     1081 caccttcagc ggaccgtcaa tgtttggcac aaaaggcagt acgagcccga caaggagcag
     1141 ccagagagct atcaccaacc acggtcgcct gcaggcagac cgtgcgagat attcgaacac
     1201 taaaccaccg tcccggcttc gactctcacg ttactccctg gtgtaggaca cgaacgtgcc
     1261 gtcctcctgg cgccttgcaa ggagaagccc gacgacggag ttggtattca gcggaccata
     1321 gatatggggg aaaacttcat ctggatcgtc atatcgaacc ggcgattcaa ccttggatag
     1381 atcaaggatg agcacacgga actgtcgttc atcggctttg tagtatcggt tgccaactgc
     1441 aaccagctca tcgagaccgt tggtacagtg gatgaagccc tcccggtcat agccctcagg
     1501 gagatacgtg ggcgcgtcgc tctgacgatc ccatacctcg ctggggacca ggtgataggt
     1561 gacacccttt tcacggcatt ccgtgctgcc agcatatctc aacgatcgct ccctcaccga
     1621 ccggatgccc atcggtgccg atagccgcga catccatcac ccctgattgt atgatgcgct
     1681 tgcacgattc ccaatcggtc ccgctccagt tcctactgga aatcgaacaa tgtgtctcag
     1741 tgagggtgac atgcgcatcg gtattatcat caatccggga tcaggtcgcc ggagcgcatc
     1801 tcgccacaga tctatggtcg agcagatcgg tgcggacgtt gcagccgatc tttcccttgt
     1861 cgtgtacgac gggtcagaac ggatcgagtc gctggtatcc cgcatggtaa gcacgatcga
     1921 tatactcggt attgtaggcg gggacggcac gctcaacggc gccatcaacg gagtcatgcg
     1981 ttcggaaaat ccagaagtgc cggtcttttt tcttcccgct ggccgtggcg gcgacactgc
     2041 cagaaccctc cccgcgctcc cgccaaatta cgcgaacgtc cggtcgttct cctaccgaac
     2101 ttgcaaaatc gatctcgggc tcctctccac gcagggagga gaatcaaggt actttatcaa
     2161 cgagagcagt attggcttgg gagcgtttgc ggcaaggtca gcgagcagac taccgcgcaa
     2221 gattggcagt accgcatatc tcattggaac gatcgctggc ctcttccgat ccccgacaac
     2281 cgatcttcgt ctggtcatcg acggcacagg cactgtcgaa cttccaggct gtcatcacct
     2341 gaccgttgcc aatggacgat actttggggc aggacttcaa atcgctccta gcgccagcgc
     2401 tgtagacgga ctcctcgata ttatcgctgt agcggacgct ggagcgtttg gtatcgcgcg
     2461 tgccctgccg cggctcctga gatccaccca tctcagtcac ccgatcgtgc ggcattggaa
     2521 gagcaccggt gt
//
LOCUS       DASYWM010000208         3401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_665276, whole genome shotgun sequence.
ACCESSION   DASYWM010000208 DASYWM010000000
VERSION     DASYWM010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665276"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(428..1036)
                     /gene="nadD"
                     /locus_tag="VGR29_08425"
     CDS             complement(428..1036)
                     /gene="nadD"
                     /locus_tag="VGR29_08425"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV2073649.1"
                     /translation="MPNRLGILGGTFDPIHIGHLIIASYASEAIGLGRVLFMPAQTPP
                     HKSRGDISPAEHRVAMVNLAIAGDDRLELSDFDLRSDGPSFTSDLLARVHREHPSEEL
                     FFIAGADSLRDFPTWNEPQVILKHAHLAIASRPGTVVTDAMLAAVPDLRNRSTLFESP
                     LIEVSSSGIRDRVRKGAAIRYLVPDEVQRYIRDRRLYLDAGT"
     gene            complement(1039..1815)
                     /locus_tag="VGR29_08430"
     CDS             complement(1039..1815)
                     /locus_tag="VGR29_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEV2073650.1"
                     /translation="MNDAILAILGEIAREALVWGKTLLRIALIVAVAWSVSWVARRQI
                     EPMLTRRSFGRNGALLIGRILSIAAFVGAFLAILGALGASWTGLLAVLSAFTVAIGLS
                     LQDVMKNFFAGILLLVERPFLVGDRVKVKDVEGEIQGIDIRTTLIRNKDGALVLVPNS
                     LMFTEILTNRSHFRTRRLDLAITSVSMPVDQIERRIADALSDVEGVRKPMPAPQIRNS
                     STEGTRMDISLLIDAKPEVEQSVMRLLIAQLGDSKIEIKE"
     gene            2251..>3401
                     /locus_tag="VGR29_08435"
     CDS             2251..>3401
                     /locus_tag="VGR29_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073651.1"
                     /translation="MSSRHPAAEEFPSSGMPDLPADLYGDDDAQYAPPRRPLSRKQRQ
                     GPAESPDSRSPRRQRPLRTQPDVVFEEIPRGDQRRGYRPKDTPDVTDGRTQSRERQAQ
                     RATRASQISAEGSIERDEVARNYVPSQERRRQRRQPLPADNRPQRRSHSTGTPPRYDR
                     VIEQPRMQAQGAAADVITSIRPVHGERSVPRRRHRNPAWTWKPIVMPQPRQILAQALP
                     NLLMATTAVVILALLPYMPFGLPLWAPLLMMPMLALIYFADETLHPTWARAAVVNLAT
                     VGAFFPAVIVRQSVLRVPFVEWGNGTLAMPILATIGAVVLLAALAAGSAVLSEEDPEY
                     AGIIFLPAALMVPFFAGATEITNLVTAFMVMAAVYAFSAVLTVVASMLPG"
BASE COUNT          719 a         1028 c          996 g          658 t
ORIGIN      
        1 gcggtcccct ggtcacgcga gaggacatct cttgcgagcc agactgcaaa gccgatcgcc
       61 accagtccga agaccacgac cggcaaggca taaagaaaca actcgttcat gcgtccctcc
      121 ctggaacccc acatgagtga aatccggtta cgtcccaccc gcagcgtcgc cgcgcaggac
      181 attcctcgaa tcatcccata aaaccaccaa tgacggctcg ccctgtggtc agttgttatg
      241 agagctcaat tgccatctcc ggaatatgtc cagagcgctg cggggtcgtc gtcaattcgg
      301 acgcgagtcc gaggcgctgg aacgttattc gcggttctat tgcaaggagt tcggcgttca
      361 ggatcccgag acagcgatcc tcgaggagcg tctgggcggg tacatccgtc catggatgat
      421 ggatgcgcta tgtaccggca tccaaataga gccgtcggtc tcgaatgtac cgctggacct
      481 cgtcaggcac caaatagcgg atggcggcgc ctttcctgac acgatctcgt atcccagatg
      541 aggagacctc aatcaacggg gattcgaaga gcgttgaccg atttcgtaga tcgggcacag
      601 cggccagcat cgcgtcggta acaacggtgc cgggccgcga ggcgatcgca aggtgagcgt
      661 gtttgagaat cacctgaggt tcgttccagg tcgggaagtc cctgagggag tcggccccgg
      721 cgataaagaa gagttcctca ctcggatgtt cccggtgcac acgcgcgagg aggtcactgg
      781 tgaatgacgg gccgtctgag cgaagatcga aatcgctgag ttcgagccga tcgtctcctg
      841 caattgccag attcaccatc gccacacgat gttctgccgg gctgatgtcc cccctgcttt
      901 tatgtggtgg agtctgagcg ggcataaaaa gcacgcgccc caaaccaatt gcttccgagg
      961 catagctggc aatgatgaga tgaccgatat ggatggggtc aaaggtaccc cccaatatcc
     1021 caagccggtt cggcatcgtc actccttgat ctcgattttc gaatctccca gctgggcaat
     1081 gagcaatcgc atgacacttt gctctacctc cggcttggca tcgataagca aggatatgtc
     1141 catgcgggtg ccctcggtcg atgagttcct gatttgcggt gccggcatcg gcttgcgcac
     1201 tccctcaacg tcagaaaggg cgtcggcgat gcgtcgctca atctggtcga caggcatcga
     1261 cacggacgtg atcgcaaggt cgaggcgtcg ggtccggaag tgcgagcgat tggtaaggat
     1321 ttcggtaaac atgagggagt ttggaaccag caccagcgcc ccgtccttat tccggatcaa
     1381 cgtagtacgg atgtcgattc cctggatttc gccctcgaca tccttgacct tcacgcggtc
     1441 gcctacaagg aagggtcgct ccaccaggag caggatcccg gcaaagaaat tcttcatcac
     1501 atcctgaagt gaaaggccga ttgcgacggt gaaggccgaa agaaccgcga ggagaccggt
     1561 ccagcttgcc cccaaggctc ccagaatagc gaggaatgcg ccgacaaatg ccgcaatgga
     1621 gaggatgcgt cctatcaaca gggctccgtt tcgcccgaag gagcggcggg tgagcatagg
     1681 ctcgatctga cgcctcgcca cccatgacac ggaccaagcc acggcgacga taagagcaat
     1741 tcgcaggagc gtcttccccc atacgagggc ttctcgcgca atttccccaa gaattgccag
     1801 aatcgcatcg ttcatgttcg gaatcggctc ccgcgtgtac gtctatccgt ccgtacgcac
     1861 actcttatca tatggcatgc tacgtgcacc acaccgaccc ccgcaggatc gccaaaagag
     1921 ccgatatcgg cgctggaaat tggcggagcc cgcgcctaca ctgagccccg gagggggtaa
     1981 tggcgatcgg agaccggctt gctgcaatgc ggccgcggtc aatgatgcat catctgaggg
     2041 agcatccgaa ccagcccttc caacgtcaac gcgcagcaac aggtggtgtc gacaacaggt
     2101 cttcctgccc tgtagcctcg gatctgtgag atcgtgttcg ccgtcggtgc gaaaaccgat
     2161 cggcccggac gaagcatcgt cttaccgaac gtatcagcgt ggacaacccc cgattggccg
     2221 ctgatgagca atacaaggag ccagtgctca atgtcatctc gccaccccgc ggcagaggag
     2281 ttcccaagtt ccggtatgcc ggatttgcca gcggatctct acggtgatga tgacgctcaa
     2341 tatgctcctc cacgacgacc cctttcccgc aagcagcggc aaggtcccgc tgaatcaccc
     2401 gacagtcgtt caccgcgccg ccaacgcccg ctacgtacgc aaccagatgt ggtctttgaa
     2461 gaaattccac ggggcgatca aaggcgtggt tatcgtccca aagacacacc cgacgttact
     2521 gatgggcgga cgcagtcgcg tgagcgacag gcgcaacgtg ctacgcgggc atcacaaatc
     2581 tcggcagagg ggagtatcga gcgtgacgaa gtggcacgca actatgtgcc gagccaggag
     2641 cgcaggcgac agcggaggca acctctccct gcagataaca ggccccagcg ccgatcgcac
     2701 agcacgggca ccccgcctcg ttacgacagg gtgatcgagc agcccaggat gcaggcccag
     2761 ggtgcagcgg cagatgtcat caccagcatt cgaccggttc acggtgagcg atccgtgccg
     2821 cgtcgacgcc accggaatcc cgcctggacg tggaaaccga tcgtcatgcc acaaccccgc
     2881 cagatactgg ctcaggcgct ccctaacctg cttatggcca ctacggcggt cgtcattctc
     2941 gcactcttgc cgtatatgcc ctttgggcta ccgctctggg cgcctctgtt gatgatgccg
     3001 atgctcgcgc tcatttactt cgccgacgag accctgcatc cgacgtgggc gcgggccgca
     3061 gtcgtcaacc tggcaacggt gggagctttc tttccagcgg tgatcgtgcg tcagagcgtg
     3121 ctgagagtgc cgtttgtgga gtggggcaac gggaccttgg caatgccgat tttggcaacc
     3181 atcggcgcgg ttgttctcct ggcggccctc gcggcgggaa gcgcagtcct gagcgaagag
     3241 gatcccgagt acgccgggat tatcttcctt cctgccgcgc tcatggtgcc attcttcgcg
     3301 ggcgcgacgg agattaccaa tctcgtgacc gcgtttatgg tcatggccgc cgtctatgct
     3361 tttagtgcgg tcctgacagt cgtcgcatcg atgctgcctg g
//
LOCUS       DASYWM010000209         3065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_590786, whole genome shotgun sequence.
ACCESSION   DASYWM010000209 DASYWM010000000
VERSION     DASYWM010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590786"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VGR29_08440"
     CDS             <1..674
                     /locus_tag="VGR29_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2073652.1"
                     /translation="YAVVSVPLKLAVALGLALILNLGVRGISVYRLLYYLPALVPPVA
                     ATIIFTLMFTPNAGPINVFLEGIGIQAPLWLRDPPWAKPVLIMLSLWPLGIETLVFLA
                     GLKEIPQDYLDAAALDGAGPWQRFRSITLPLLSPVMLFNLVIGVIYSFQVFTQALVIG
                     GTTGEPLESTLMFMVLIYRSAFRYFSMGYAAALSVILFLAVMGLTLLIFRTTRYWVHY
                     EGGHQ"
     gene            671..1594
                     /locus_tag="VGR29_08445"
     CDS             671..1594
                     /locus_tag="VGR29_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2073653.1"
                     /translation="MSEIAQQPVTRTDQPALTHEVTNVRPAHGARFRRRMAPFAKHAL
                     LITASLIFLLPFYWMLVSALKTNSQIFARPMQWWPDPVQWSNLPDTINYPAFPYFRLL
                     WNSIYYSGAVTIGTVLSCTAVGYGFARLRFPGRNLLFAITLGTLMIPPIVTFIPTYVL
                     FAKAGLTGTYAPLILPYFLGNAFYIFMMRQFFMGVPWELSDAARLDGAGEFRIFWQIM
                     LPLVKPAVMVVAVFSILYTWHDFFGPLIYLQDRSTFPLSLGLFAFKAQRTSEWGLIMM
                     GSILTTLPLIIIFLFTQRYFLQGVRMTGIKG"
     gene            1645..2802
                     /locus_tag="VGR29_08450"
     CDS             1645..2802
                     /locus_tag="VGR29_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2073654.1"
                     /translation="MSDRIRIGLIGCGSLSQIGILPQLAEPDAREQIEFVAVCDTVAA
                     RAEETARAYDVPHAFTDPDDVINHNDIDLILVITPIGLHFPLAMQALRAGKHVYVQKT
                     MTTTYEEAKQLVATARVRNLTLVSAPGQMLAPAMQAMRTLVTSGALGDVYWAWGSTGG
                     WQHHLEANRQGDDPRSSADPSWYYSKSGGPLRDVTVYVLHSLTGILGPAKRVTAMSGI
                     RTTQHTWRDKVIPVEIDDNTLLMLDFGDVTFAVLGGHSAKTGRLIQWGGMGIYGSHGS
                     VEALEVEPLSGHPVRLSINGDADLPGWERTSEDTYTLLTHMPHVNDRHAAIPEPHVYA
                     DIMHCVDCIRTGEQPIASGEHAAHVIEIIEKAYLAAETGQAQDLESTFSLH"
     gene            2868..>3065
                     /locus_tag="VGR29_08455"
     CDS             2868..>3065
                     /locus_tag="VGR29_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2073655.1"
                     /translation="MAGRLRLALLGCGDVAQRDYLPELHRLADRVELVAVCGRTEARA
                     RQVANQYGVPGWYTDYARMLAE"
BASE COUNT          584 a          961 c          844 g          676 t
ORIGIN      
        1 tctatgcggt ggtctcggtc cctctcaagc tcgcggtggc gctggggctg gccctgatcc
       61 tgaatctcgg cgtacgggga atatccgttt atcgccttct ctattacctg cccgcgctcg
      121 ttcctcccgt cgccgccacg atcatcttta cacttatgtt tacgccaaac gcggggccaa
      181 tcaacgtctt cctcgagggg ataggtatcc aggcgcctct ctggttacgc gatccaccct
      241 gggcaaaacc cgtcctgatc atgctaagcc tgtggcccct ggggatcgaa acgctcgtct
      301 tcctggccgg actgaaagag attccgcagg actatctcga cgcagcggcg ctggacggtg
      361 cgggaccgtg gcaacggttt cgaagcatca ccttgcccct cctctcaccg gtcatgctct
      421 tcaacctcgt catcggcgtg atctactcgt ttcaggtctt cacccaggcg ctggttatcg
      481 gcggcacaac gggcgaacct ttggaatcca cgctcatgtt tatggtgctc atctatcgca
      541 gtgcattccg gtacttctcg atgggctatg cagcggcgct ttcggtcatc ctgttcctcg
      601 cggtaatggg tttgactctc ctaattttcc gtacgacccg ttattgggtg cattacgaag
      661 ggggccatca atgagcgaga tcgctcagca acctgtcacc cggacagacc aaccggctct
      721 cacgcacgag gtcacaaacg ttcgtcctgc ccatggcgcc cgctttcgcc ggcggatggc
      781 gccattcgcc aaacacgcat tgctcatcac agcgtccctg atcttcctcc tgcctttcta
      841 ctggatgctg gtttccgcgc tgaaaaccaa ctcccagatc ttcgcccggc caatgcagtg
      901 gtggcccgac cctgtccagt ggagcaactt gccagacacc attaattacc cggcatttcc
      961 ctacttccgg ttgctctgga acagcatcta ttactccggg gccgtgacca tcggcaccgt
     1021 attgtcatgt accgccgtcg ggtacgggtt cgcgcggctg cggtttccag gccgcaattt
     1081 gctgtttgcg attaccctgg gcacgctgat gattccgccg atcgtgacct tcattccgac
     1141 gtacgtttta ttcgcgaagg ccgggctcac aggtacctac gcgccgctca tcctgcccta
     1201 cttccttgga aatgcctttt acatctttat gatgcgccag ttctttatgg gtgttccctg
     1261 ggaactctcc gacgcagccc gcctggatgg cgccggcgag tttcgcatct tctggcagat
     1321 catgctgccg ctggttaaac cggcggtaat ggtcgtcgcc gtattttcca tcctgtatac
     1381 gtggcacgat tttttcggcc cactgattta cctgcaggac cgcagcacct ttccattgag
     1441 ccttggactt ttcgccttca aggcgcagcg cacctccgaa tgggggctca tcatgatggg
     1501 atcaatcttg actaccttgc cgctgatcat catcttcctc ttcactcagc gatatttcct
     1561 gcaaggtgtc aggatgaccg gcattaaggg atagaactgc ataatcggac gctcggctct
     1621 cgtctcaaga gaaggacagg ctccatgtcg gatcggatac gtatcgggct tatcgggtgc
     1681 ggaagcctct cacaaatcgg cattctgccc cagttggccg agccggacgc gcgtgagcag
     1741 atcgagttcg tcgctgtgtg cgacacggtg gcggcacggg cagaagaaac ggcgcgagcc
     1801 tacgatgtcc ctcacgcctt caccgatcct gacgatgtga tcaaccacaa cgatatcgac
     1861 ctcatactgg tcattacccc gataggtctc catttccccc ttgcgatgca ggcactgcgt
     1921 gctggaaagc atgtctacgt ccagaaaaca atgacgacga cgtacgagga agccaaacaa
     1981 ctagttgcca ctgcgcgcgt acgaaacctc accctggtct ccgcgcccgg ccagatgctt
     2041 gctccggcca tgcaggccat gcggacgctg gtgacctcgg gggctcttgg cgatgtctac
     2101 tgggcatggg gcagcacggg cggatggcaa caccaccttg aagccaaccg acaaggagat
     2161 gacccgcgaa gctcggccga tccctcctgg tattactcca agtcaggcgg gccgctccgg
     2221 gatgtcacag tgtacgtgct tcatagcctg accggcatct tgggacccgc gaaacgggta
     2281 accgccatgt cgggcatccg cacgacccaa cacacgtggc gggacaaggt cattccggtg
     2341 gagattgatg acaataccct gctcatgctg gattttggtg acgtgacctt cgccgtgctg
     2401 ggaggacaca gcgcaaagac cggtcgcctg atccagtggg gaggtatggg tatctacgga
     2461 tcccatggct cggtcgaggc gctcgaggtc gagccactct ccgggcatcc tgttcgcctc
     2521 tcgatcaacg gagacgctga cctgccaggg tgggaacgta ccagcgagga cacctacacg
     2581 ctgttgacac atatgcccca tgtcaacgac cgccacgcgg ccatcccgga accccacgtc
     2641 tatgccgaca tcatgcactg cgtcgactgc attcgaacgg gtgagcagcc tatcgcatcc
     2701 ggcgagcacg cggcccacgt gatcgagatc atcgaaaagg cctacctcgc tgccgagacg
     2761 ggccaggcac aagatctgga gagcaccttc tcgttgcatt gagcggccct atgctaacgc
     2821 gacatgtgac atcaccgaaa tgagttccat ctggaaaggg agtgcgtgtg gccggacgac
     2881 taagacttgc cctgctcggc tgtggcgatg tagcccaacg agattacctg ccggaacttc
     2941 atcgcctggc cgaccgggtg gaactcgttg ccgtctgcgg ccgcaccgaa gctcgcgccc
     3001 gccaggtggc gaaccagtat ggagttcccg gctggtatac cgactatgcg cggatgctgg
     3061 ccgag
//
LOCUS       DASYWM010000210         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_121371, whole genome shotgun sequence.
ACCESSION   DASYWM010000210 DASYWM010000000
VERSION     DASYWM010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121371"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(544..1617)
                     /locus_tag="VGR29_08460"
     CDS             complement(544..1617)
                     /locus_tag="VGR29_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2073656.1"
                     /translation="MTDTPSSSDVVIVGAGIAGLSAARALTRVGVQVIVLEARDRVGG
                     RMHSLNLSRGAIDLGATWFWPNEPLVQLLLNEYELQAFPQEAAGDTLYIQDSGRTYRL
                     PRDVGGSTSYRFTAGAQALPMAMASELPDGCLRLNSPVHRIMTDADSLTVESASGTVT
                     TRNVIVAIPPALALEAIAFVPPLPDALDSIARQTAVWMGDMVKAVAIYNRPYWREQGL
                     SGFVFSQSGPFRELHDHSTVGDRAAAIFGFSPAESLVPGDSSTIREAFVRQLVSIFGG
                     EAADPLDVIVTNWAAERYTSPRQWRTSKTTATYGHPAFREEGRNVQILWASTETADAF
                     AGHMEGAIRAGIHAAAAVRQASD"
     gene            1827..2507
                     /locus_tag="VGR29_08465"
     CDS             1827..2507
                     /locus_tag="VGR29_08465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2073657.1"
                     /translation="MHDAREQQQQHWEQTYTDMPQMYGDAPSAPALIAAERFREEEVV
                     TILELGGGHGRDSLYFARQGFRVTMLDYAQPGVDALVDQARELDLSANVTARQHDVRR
                     HLPFPDASFDACYSHMLFSMAISTAEQTALAQEVWRVLRPGALHIYTVRHKGDPHFQV
                     GTHHGEDRYETGGFEVHFFDRAKVLSLATGFDLLDIDTFEEGSLPRRLFRVTLRKLDD
                     QCGSGAGR"
     gene            complement(2636..3001)
                     /locus_tag="VGR29_08470"
     CDS             complement(2636..3001)
                     /locus_tag="VGR29_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2073658.1"
                     /translation="MTTPEPTSPAMASQGEMISARLAEIGMSVSHFARALGVRSSFIH
                     AVRTGKRRLTKPATVERAAAVLGISADRLYVAGGHLPPDAWTIIKRRPQLVRALRVLD
                     AKLDAREGQFVSRPRMNTR"
BASE COUNT          603 a          932 c          915 g          612 t
ORIGIN      
        1 ctttccgcgt gcgtggagac gttgcgcacg tctagctggc cacgacctgt caccaaggtg
       61 gctgtcggcg tttttgacgc cgttcacgac atggttctcc gtgcgggtgc gtggaaatga
      121 tcgccccgcg tccggtcccc gcgcctacac gatgacgatc aatgaggaga atgcgcgttc
      181 cttgtatagg gaggcatacc aggggtagtt gtctcacttc ccgcctaccg atgcacctag
      241 tgagacagag attctgagac gggttctgca ccctgaatag gacggtatat cggctcccag
      301 tcagccccgt acaaggtgtc gcagaaacga ccgtttctgc gactgtcaac catcctgtcg
      361 ttaatccctg aggcaacggc gagggccata atcactggac ttgcaccaag cagtcgtgtc
      421 ccttgcgtgt gcttgaagct tcctcgaagg acaaagccat gtgatgaacc tttgacgaga
      481 aaccgttgcg tcgacgagaa ggggctggtc gccttaaccc ggtggggtta cggggcggtg
      541 acattaatca ctggcctgac ggactgcggc ggcggcatga attcctgccc gaatcgcccc
      601 ctccatatgg ccggcaaacg cgtcggccgt ctcggtcgag gcccagagaa tttgtacgtt
      661 tcgtccctct tcgcggaagg ccgggtgacc atatgttgcc gtcgtcttgg atgtgcgcca
      721 ttggcggggt gacgtgtagc gctcagccgc ccagttcgtg acaatgacat ccagcgggtc
      781 ggccgcttcg ccgccaaata tgctgaccag ctgacgaacg aatgcttccc ggatcgttga
      841 gctgtcacca ggcaccaacg attcagctgg ggagaaccca aagattgctg ccgcgcggtc
      901 gccgaccgta ctgtgatcat ggagctcccg gaacggacca gactggctga agacaaatcc
      961 ggacaacccc tgttcacgcc agtacggtct gttatagatc gccacggctt tgaccatgtc
     1021 tcccatccac accgccgtct gccgggcgat gctgtcgagc gcgtctggta atggagggac
     1081 gaaggcaatc gcctcgagtg ccagagcggg tggtatcgca acgatgacgt ttcgagtcgt
     1141 gactgtcccg gacgccgact cgacggtgag gctatcggcg tcggtcataa tccgatgcac
     1201 ggggctgttg agccggagac atccgtccgg gagttccgat gccatcgcca tggggagcgc
     1261 ctgtgcgccg gctgtaaagc gataagacgt gctcccaccg acgtctcggg gcaggcggta
     1321 ggtacgcccg ctgtcctgga tatagagcgt gtcccctgcg gcttcttgtg gaaacgcctg
     1381 gagctcgtat tcgttcagaa gcaactgcac gagcggttca tttggccaga accacgtcgc
     1441 tcccaggtcg attgcgcctc tgctgaggtt gagactgtgc attcgcccac ccacacggtc
     1501 acgtgcctcg aggacaataa cctgcactcc gacacgagtc agcgctcgcg cggcactcaa
     1561 tcctgcgatg ccggcgccga cgatgaccac gtcgctcgaa gatggagtgt cagtcaccag
     1621 cggcccccgg gacaagaaca aaatcggaac tgggcatccc cgtcacgaag tcgcgcaata
     1681 gcctcattca cttgtgcgag cgggaggcgc tcgacccatg ggcgaattct ggcggctgta
     1741 cgacctgttc taccaccatt acggcatgcg acaactacgc agctgcattg accatcgcgg
     1801 tcgcacatgt ctcagtcggg aggcctatgc acgacgctcg tgaacaacaa cagcagcact
     1861 gggagcaaac ctacacggac atgccccaga tgtatggcga tgctcccagc gcccctgcgc
     1921 tgatcgccgc cgaacgattc cgggaagaag aggtggtgac catcctcgaa ctcggtggtg
     1981 gccacggtcg ggactccctg tattttgccc gccaagggtt tcgtgtcacc atgctggact
     2041 acgcccagcc cggtgtcgat gccctggttg atcaagctcg tgaactcgac ctttccgcga
     2101 acgtaacggc acgtcagcac gacgttcgac gccaccttcc ctttcctgat gcatcatttg
     2161 acgcctgcta ttcccacatg ctcttcagta tggcgatcag caccgccgaa caaacagccc
     2221 ttgcccagga agtgtggagg gtccttcgac ccggggcgct ccatatttat actgtccggc
     2281 acaagggcga tccacacttc caagtgggca cccatcatgg agaagatcgt tacgaaacgg
     2341 gcggctttga agttcacttc ttcgatcgag caaaggtgct gtccttagcc actgggttcg
     2401 acctcctgga cattgatacg tttgaggagg ggagccttcc acgtcgtctc ttccgcgtga
     2461 cgctgcgaaa gctggatgac cagtgcggca gcggggccgg ccggtgaggc cgaagacgat
     2521 gcaggagtag gtgtacggca agttcaagcg tttcgaggga tgagtggtgt gcggataatt
     2581 taccttcgcc gcagcgcacc ccaaccagat gaagagttgc gaccctggct atcgcttacc
     2641 gagtattcat ccttggccgc gacacgaact gcccctcccg ggcatcgagc ttggcgtcga
     2701 ggacgcgaag ggctcgcacc agctggggac gacgcttgat gatcgtccag gcgtcgggcg
     2761 gcaagtgccc gccggcgaca tatagccggt cagcactgat gcccaacacc gcggcggctc
     2821 gctccacggt cgctggcttg gtaagccggc gtttgccggt ccgcaccgca tggataaagg
     2881 acgacctgac tccaagcgcc cgggcaaagt gggacacgct catcccgatc tcagctagcc
     2941 gcgcactgat catctctccc tgggatgcca tggcgggaga tgtcggctca ggtgtggtca
     3001 ttggtggcct cctcatcaag caacgtgcgt cgtccatatt cgtcaatggc gtaccggctc
     3061 ac
//
LOCUS       DASYWM010000211        28761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_403271, whole genome shotgun sequence.
ACCESSION   DASYWM010000211 DASYWM010000000
VERSION     DASYWM010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 28761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28761
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403271"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VGR29_08475"
     CDS             <1..465
                     /locus_tag="VGR29_08475"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEV2073659.1"
                     /translation="GKIVQLGTPMEVYERPATEFVGGFLGSPPMNFLKVQLENSDGQP
                     RAALGGHSLPVPRALAERGERDLLMGIRAENIEATPVNGQTEADALVSRTLVVELLGS
                     QLLVTGQVGDQPIKVLVRADAPIKPGDDLQLRPEEDKVRWFDTTTRQEIVLS"
     gene            613..1707
                     /locus_tag="VGR29_08480"
     CDS             613..1707
                     /locus_tag="VGR29_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073660.1"
                     /translation="MTSSLFNGNVRGNPYRIRDPHNSILTEIWLVSAIVSVLLIRLYL
                     YLTGYPQVGGETLHIAHMLWGGLGMVVSFALVILFAHPVWKPIAALVGGVGFGAFIDE
                     LGKFITKDNDYFYEPTVALMYGIFIILFFLARYFDRKREPTEADHLYLAVQGIQWQAI
                     GKLDRHRQEAALGHLEASGNRSALAGHLRQILQNAEIAEDAGRSRIVALREKGAELYW
                     SVVGHKWMRRVIVGLFIIRALQILGAVIIGMVSDTFIFTDGLSISEWLAMAAGGVSGG
                     LALYGVVRIFQRRRVDAIQALAGSALVSLLFGQFFAFASVQFVALGGLVLDLGALGAL
                     RLALAAESERHEREDGEAEDDPRQGLGQFL"
     gene            1738..2598
                     /locus_tag="VGR29_08485"
     CDS             1738..2598
                     /locus_tag="VGR29_08485"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEV2073661.1"
                     /translation="MNQSAMPPVDVMDLFPEVREGGVRILQSLSEQEWRLPTACAGWS
                     VHDVALHIGGGLLANVSRRRDQHVGNFREFEPPNNATLDEHERLIHTLNGWNEMWVLA
                     GRRISPALLIEIIDSAGCQLEEYFRRLDFRVLGDPVGWAGLQPAPVWLDVAREYTELW
                     THLAQIREATGRAVVDAPHLLAPVLATFAHGIPHALRSIDRPMGATLTVVVHGEAGGQ
                     WWVKRAASGWELHGEVTGSADAVIDLDQVTAWRLATKGMSRDDARREVNIKGDIELGE
                     GFLNLVAILA"
     gene            complement(2614..3546)
                     /locus_tag="VGR29_08490"
     CDS             complement(2614..3546)
                     /locus_tag="VGR29_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2073662.1"
                     /translation="MTASTPRHEAQATFTEVMNSHFSRLGGTVGVMVHEFVSGRELTY
                     NHTEIFPTASTLKVPLLYELYRQADEGVIDLTERVTLRHSDRVPGSGVLQDLDEGLQP
                     TIRDLAELMIIVSDNWATDMIYARIGKENMARTLTNLGLHQTHIPMNINQLFCTLAGV
                     EWDDPKVDYHFLKEYLKDYTPAVNNVGYAADERNDVSSPADMVRLLVQIHEGHGLTRE
                     SQEGVIRILKDQNFNTIIPARLPQDEHIETAHKTGSLRGIRNDVGIAYSPKVNYAVAF
                     MSKGQDDTAEAVDRMARASRWIWDYLVGVHDPKV"
     gene            3723..4790
                     /locus_tag="VGR29_08495"
     CDS             3723..4790
                     /locus_tag="VGR29_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2073663.1"
                     /translation="MGNESTLYKWLKIGGGIGAALGVREAIGRAREVDLSGQVALVTG
                     SSSGLGLLMARELAGEGCRLVICARSEPELEQAGRDLESRGAEVLAVRCDVADKDDVQ
                     RMIDAAVDAFGGIDLLICNAGVIQVGQFQSMELKDFHEAMDIMYFGTLYPILAALPRM
                     RAKGRGRIALVTSIGGKISVPYLLPYNAAKFAAVGLGEGLRAELGDEGIIVTTIIPGL
                     MRTGSYLNAKFSGEVSGRESAYRLFSALSSVPLLTGNAESAARAYIRAIKRGDAYVMY
                     PPQYNLIARIHGVAPATTMAAMGLADRLMPKTGGDTGTVPGASIDSRLGSGGIWRTLT
                     TLGRKAASRMQPEPGPPDSGS"
     gene            complement(4803..5846)
                     /locus_tag="VGR29_08500"
     CDS             complement(4803..5846)
                     /locus_tag="VGR29_08500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2073664.1"
                     /translation="MEIGIYSFADVTEDSSTGTMITPQERLRNLIEEIELADQVGLDV
                     YGLGEHHRPDYASSAPAVVLAAAATRTKRIRLTSAVSVLSSDDPVRVFQQFSTLDLLS
                     GGRAEIMVGRGSFIESFPLFGYDLNDYNELFSEKLDLLLRIREEEHVVWSGRTREALT
                     GQGVYPRPVQDPLPVWIAVGGTPQSAVRAGTLGVPMAVAIIGGETRKFVPLVNLYREA
                     LRRGGHDPLTVPVSINSHGYVAESMQQAIADSFPAHKVMMDRIGRERGWGELSREQFA
                     AGAFLGGALVAGSPEDIAEKILYQHRLFNHQRFLLQISVGTMPHNKVLRSIELLGTKV
                     VPLVREALKQQGE"
     gene            6031..7167
                     /locus_tag="VGR29_08505"
     CDS             6031..7167
                     /locus_tag="VGR29_08505"
                     /inference="COORDINATES: protein motif:HMM:NF037012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEV2073665.1"
                     /translation="MSPKNPERSTLRQIRNALQVLHKALLEAERLEYERSFGRIQSDF
                     YLLTLAAEDPQFAWLRALTNLMLEVDMHLSGETQVSPEDLRLTGDRVRALLIPDDAPT
                     PFQARYQHAMQQHPAVIMAHSRAIRSLPPASRTPLLKGGAPTDTQHFGDLRVQLHHPG
                     ELVPGHGDHGYGPLAVVAESSMAPGWVVPMHLHRNDEIVSWVPEGVMRHDDLAHGKLV
                     TDGDHLLVMNAGRRFSHAERTLETDSSLRMLQIFVRPRAVDLEPRLQHGALPVAEENA
                     WRLIVGPEGEDALFSVRNEVQILDARMVQGVYISLPQREGWLTWLFVYRGAVEIDGVR
                     LGQSESVLVGSAGPVEIGAVEETLLIVFVINLTAKITRSGTVRR"
     gene            complement(7232..8158)
                     /locus_tag="VGR29_08510"
     CDS             complement(7232..8158)
                     /locus_tag="VGR29_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV2073666.1"
                     /translation="MAKGSKKVVLAALGANAAIASGKLAVGLLTGSAAMLAEAAHSAA
                     DTVNQVFLLVSITLSDNPADEEHPYGHGKERFFWAFLAAIFIFVAGAFFSIYHGIEKL
                     MAGGEHHGAYWPSYLVLGIAFVFDLIVLILALRTAQSEAEAVGMGTRDYLRETTDVTL
                     KTALYEDFAATTGVVIAAGGLFLHQITGNAIYDGIASILIGIILVAVAIMLGKETRNL
                     LLGSSASPRARKAIRGAIEEFPEVTHVVNLLTMQLGLDSILVTGEITIEDRLTTDEIE
                     RLLRRISARIHEAAPAVRNIYLEPHSTPRGAS"
     gene            8551..10842
                     /locus_tag="VGR29_08515"
     CDS             8551..10842
                     /locus_tag="VGR29_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073667.1"
                     /translation="MKEKSFFERVVMLLGVLLSLLVGVAKLLFSLFNGLRKVFTSEQG
                     PGAGTDISRVGDQAKQFVSGAAEKGQGLVEDVKQRAGDLLSRESESESEGQDGPVTEP
                     DVTSGEDTVVAGFGEPNEELSEHQHEDIGGVTNGVDVNTSAQSGGFVTSRGSDVSDQT
                     VSAAEPSDYGSGINEGVPGGLYEEDLNRQDEPRLNEGIPNLGEPVGVEGEDFGTYEEE
                     MVEEVVAEGDEDDDTDNLFASNQPFIVSDVAGQADIPAASRGLREPGEEIGDELDIED
                     ISDTDNIDPYEDFAPSGAGGEVDLENDESPSWDESEMTIVESPDDPVSADEIGAEDDD
                     TPTLGLRDSESLTIAGTNDDVSGAPMPEFRGVETRDDDHLGVTGDFDQADATETIVAA
                     PGDTDTVHSTHADPREDFVTGDAADLAISDVAGDEDFDSAPTGGEYGETDAFGMPETT
                     DVSTGDVGSDDVVASDAFDRFITGHAAEVPDELSTDDDHLGISGNFGEADRTETIPAA
                     PADPETAAEEEEPQQETFVTGEDGDADTGSSGEGGADPLPSGATGQGSGPSAAWSTSA
                     GFLSTELTQPSDVNIPGGDEGGSGGSEELPTGEAGGGGAGQGDGAMDVDVASSGAAGE
                     SPAAAINQHLDVVQDNLEESLADDAADVASASTGDLGTTTGPDVSATAGTARQGGDTT
                     PDSGSSRKSRKQRAQDSDWVPNGAVKGDGSHTCPAEYPIKGNANSRIYHRPEDSSYDS
                     TIAEYCFASEQDATAAGFRARKG"
     gene            complement(10928..11167)
                     /locus_tag="VGR29_08520"
     CDS             complement(10928..11167)
                     /locus_tag="VGR29_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073668.1"
                     /translation="MSDRNTSNDVDRTTEPLEGEFQPVEQFPPTIATDGEPEDELGPE
                     LDPNGSDMESNVTGRSKPASDGVAEEERDERLSNG"
     gene            complement(11359..11607)
                     /locus_tag="VGR29_08525"
     CDS             complement(11359..11607)
                     /locus_tag="VGR29_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073669.1"
                     /translation="MLVDVVISAARLAFLQEGRWLPGTKDLFAELDVLDPPLGLQVRN
                     AVCTGSTVEYLALVSQIVDHVVDATGFVQWKSDRQPAE"
     gene            complement(12093..13079)
                     /locus_tag="VGR29_08530"
     CDS             complement(12093..13079)
                     /locus_tag="VGR29_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073670.1"
                     /translation="MASAPLSRRAFVAATAVIVCGSPLPHMLSGQERDVHGDPMVAAE
                     EISKLEVISHIPALYEFYALMHPDAQAIVPHHVVIRYFREVFQDRDPKPAVATGVTYK
                     NWTWGVNGVTYPGTAEVSFRQEYGDGSVEQDVVRLVHHDNTRKWFFGRDRAWVDQQIQ
                     CFTQTLNVPQEGTVPYGLENAVLSDDLLNRLPKSLGDGPMKGTLAHLEPHETRLPDWA
                     GGEVSLRYESSEHYPVGYVQLLSLKPETNIPEAIRGEIETYETAPPFTLHGWNLLPGN
                     AIPFAHYEIFGSDAVGNALSVMWGRAGGDRIAVISHVDEATLEAIAPALLDA"
     gene            complement(13126..14634)
                     /locus_tag="VGR29_08535"
     CDS             complement(13126..14634)
                     /locus_tag="VGR29_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEV2073671.1"
                     /translation="MDSIASLMDGFSIALTPQNLLWALVGVFLGTFVGVLPGIGPALT
                     VALLLPVTYALDPIPAFIMFAGIYYGGMYGGSTTSILLNTPGESASIITALEGNRMAA
                     KGRGAAALATAAIGSFVAGTLATIGLTLVAPAMARIALRFGPAEYLALMVLAFTTVAT
                     LLGSSMVRGLVSLFLGLAIGLIGIDALTGQPRFTFGNPHLLNGVDVVTVAVGLFAVGE
                     TLWVASHMRREKQKTQEISGSKWMTKEDWKRSWRPWLRGAGLGFPIGALPAGGAEIPT
                     FLSYSIEKRLSKHPEEFGKGAIEGVAGPEAANNASAAGVLVPLLTLGLPTSATAAIML
                     AAFQRYNLNPGPQLFDRNPELVWGLIASLYIGNLMLLVLNLPLVGLWVKLLSIPRPWL
                     YSGILVFAGLGTWAQNQSVTGLIVLYIIGVVGYVMRRFDIPVGPAVVAVILGPEAETQ
                     FRRALQISQGDYSVFVTRPIAATIFAIALLALIGPTLWRTTRRSGNVDATAT"
     gene            complement(14846..15403)
                     /locus_tag="VGR29_08540"
     CDS             complement(14846..15403)
                     /locus_tag="VGR29_08540"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEV2073672.1"
                     /translation="MVIEPDTGPGPSPADDNVDVPTGLARWSEILIPVVLVIAGIIIL
                     VQTQDIRVIRSMSQVSPRAIPNLVGGGLIVVGVWYAIDIIRKPHVLTAGEDAEDVDID
                     APTDWGVLAIIGIGLAIFALLIRPAGFILASAILFAIASTAMGSRRIIANVLIGLILA
                     SAVFLLFDTWLGVRLPAGILEFVLE"
     gene            complement(15475..16548)
                     /locus_tag="VGR29_08545"
     CDS             complement(15475..16548)
                     /locus_tag="VGR29_08545"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEV2073673.1"
                     /translation="MSTSQTSWHPLRALGLLVIAAVLALGAMVPGMAFAQGDAVDCTE
                     WSSETLQIMAPAAPGGGWDSTARELARALQEEGIVENVEVFNVEGAGGTIGLAELVNT
                     HAGDGHMAMVMGLVMIGGIGLNESPVTLEQVTPLARLTTEFEVIVVPADSPYETIDDL
                     MAAFQEDPGAVSWAGGSAGGTDHLLVGLLAQSQGIDPTLINYVPFSGGGEALSALLGG
                     QTTAGVSGLGEWQAQIESGDLRALALSGRADSGDATPVAGGATPEAFGADIPTLQESG
                     IDIELANWRGIVAPPEISEEDQACLNQAIEAAVNSETWQNTLRSMGWTGFYLGGEEYV
                     AFIQSEIERVNTILTDLQLIGGE"
     gene            complement(16828..17043)
                     /locus_tag="VGR29_08550"
     CDS             complement(16828..17043)
                     /locus_tag="VGR29_08550"
                     /inference="COORDINATES: protein motif:HMM:NF017917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HEV2073674.1"
                     /translation="MPYKNRGDLPESVKDNLPKHAEEIYKEAFNSAWDEYGHDEARAH
                     RVAWGAVKKKYHKNDEGDWVEGPSEDD"
     gene            complement(17101..17913)
                     /locus_tag="VGR29_08555"
     CDS             complement(17101..17913)
                     /locus_tag="VGR29_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2073675.1"
                     /translation="MPRQFDHFVIISADLKTAMDNAQRAGFTVVPGGTHGDGRTHNAL
                     IAFADGAYIELITPTSATPEGEHRWFPRLFAAGGLVDFCLLGDQLEDEIAAIRDQGVE
                     YPQPFSMGRNRPDGKRIDWLLSVPPGPVGQTGWPFLIEDVTPRELRVPRAPDEVRHPN
                     GVLGAAGVTILVHDLDASVRDYTAILGTAGHELTAPFSDDRLGVVFPVGRERDQWIML
                     VEPKAHEAAEHLNRHGQGPYRLTLRTHDGAIGPGEGDFVDPALFNGARILLA"
     gene            18090..18959
                     /locus_tag="VGR29_08560"
     CDS             18090..18959
                     /locus_tag="VGR29_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEV2073676.1"
                     /translation="MTPHNRHLHSTSNLSRRHFLTGAALGVASLALPQSPHAVSAQSM
                     DPFTFITAMKTTTDVGQVVGQAASEIERVSADGWYWDAYIQVPVKEGQDFHFTCEFDS
                     AWIILKAYGFDLTLEEQMAIVGVDNAIEPWYEETPTGIIVWGGDIDEHYCGHYDTNVL
                     TRARCNAMRKVFESVGLGVTFTPERRLIEEALLRGEPVFFKSTVDFLPWRAATWKTPD
                     GDDFPVVLTNDHALTVMGFNADEVIIRDPLGPTTTNYKRPWQYRVSWGRFLEVIAAQG
                     NDAIAIAPSPPLT"
     gene            19037..20461
                     /locus_tag="VGR29_08565"
     CDS             19037..20461
                     /locus_tag="VGR29_08565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2073677.1"
                     /translation="MSRRPARHYDLIVIGGGASGSLVASGAVDEGLSVALIEEWKLGG
                     TCLNAGCDPTKAMVRTAEVLHLARTASRFGISVPKARLEWEALRTRVDDLIDEIRGGD
                     GPANVRAQGIDLYKMHGKFLSENEVLAGDSLLTGDRVLISTGQTARVPQVTGLEQTGY
                     LTNVDAVSLDELPESLLIIGGGAVAVEFAQMFSRYGAEVTIVGTQEHVLPKEDEELSG
                     ELTRILRDEGITLQMRSRATGIRRSPDGRVILTCSRDDGPPVELAADEVLVATGRVPN
                     IAGLDLDVAGVRFGERGILVDAQMRTNVPHIFSVGDVTGIYPFTHVADYQARIALHNI
                     VNEDGAMKADYRVVPWVTFSDPELARVGLTEAEARAGGYSVVTSTVPFAEMTRAMTSD
                     QREGLVKLVVDRATHRVLGGHILGAGAGELIGEIAIVMQYRLPVSALSDTIHPYPTMS
                     EAIFKAAHDLMQGALAGTSPVNVG"
     gene            complement(20476..21486)
                     /locus_tag="VGR29_08570"
     CDS             complement(20476..21486)
                     /locus_tag="VGR29_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073678.1"
                     /translation="MDFGAFSIISLIYVVIGVRVVAQLVRSWRATWDHHFTPTDRHLV
                     DQAAFFVFIPISVALHELGHAVAVWSFGREVVEFGFYGFAGYVAYIPFGLSDVQQTII
                     AAAGSLVNLMLCVIGLAIVLLKKPPMRAAFNELLIQFVLISGLNAFIVYPVLDMLSGL
                     NGDWRQMYDSGVPWLSGIIVAVQILILAAGYWLLTNPGMRARIARLTDIPPGYQRGLL
                     GGVQVGKVHPKAYSPAEQTLHEAVDRVASGWPSRVTTSVQRFPAGSAITLQWNDGRQQ
                     HVVAARVFTSGITEIIRVPMPSAPGGAQSARMLHRWTDLPGTDELTIGLRIAMETTQQ
                     QG"
     gene            complement(21704..23044)
                     /locus_tag="VGR29_08575"
     CDS             complement(21704..23044)
                     /locus_tag="VGR29_08575"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073679.1"
                     /translation="MSSKNSQSSRTGGRDENVTAPDARSGGLSRGFAALKYRNYRLFF
                     TGQLISVTGTWMQSLAQSWLVVDRLDASAFQLGLVNVFQFGPVLLLGIPAGVVADKIP
                     KRNLMVVTQVIFGILATALSYLVFTGQVELWHVYLLAGFFGVTNAFDMPARQAFVSEM
                     VDKEALMNAIALNSAMFNTGRILGPAVAGVILAAFGPAVCFAVNATSYLAVIAGLLMM
                     RITKVIQATTGSAGEQLREGIRFVRNTADIMRPITLVAFVGTFGMAYNVWLPLLVTDS
                     FKADAGIFGLLFSSMGAGSLIGALSIAFSGNKPSRVRMIVAAIALGIVILALAVTASI
                     PLPVGVAMLLLASSGFAASNAMAMANTIVQTTAPDELRGRVMAVYSTVFMGSVPIGAL
                     IAGIVSDRFSVITSMTFGGTIVTLVAITLFWLQRSPRARAAAPQTMTTSPVPKR"
     gene            complement(23344..23811)
                     /locus_tag="VGR29_08580"
     CDS             complement(23344..23811)
                     /locus_tag="VGR29_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2073680.1"
                     /translation="MYNRVIVPLDGSALAEEALSQAWKLATTMNIPLHIVRVVDTYRA
                     QTVPATGMALDYSMLAELAEEEFEDARSYIEDQIGKLSEEGLTVTGEVLHGPIAQQIV
                     NSADEGDVIVMSSHGRTGIKRWFLGSVAEEVMRRATCPVLLHRASAARKQGDQ"
     gene            23975..24805
                     /locus_tag="VGR29_08585"
     CDS             23975..24805
                     /locus_tag="VGR29_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEV2073681.1"
                     /translation="MSDIHRQSDQMPQSQHWEAAEPILLANLTSPQTGQLLQSIEMVL
                     IPVGAHEQHGPALPVSTDALTAQVLCALTGALLAPRVAVLPVIPWGVSWHHQGRPGTV
                     TLREETLISLVLDQIDSLHRQGVKRIVLVNTHGGNNPALAIAAERAKRELGVPLVVSV
                     YAYTLLANAAREILGDDAIGHGGGDEAAAVLAIRPDLVDGDRLGPQQLNSAIQRQKTI
                     VSAVGGTLPVMMHKLTESGATGNSINATTEAGHAILGQAASGLRAVCEELLEADLAQL
                     "
     gene            24931..26970
                     /locus_tag="VGR29_08590"
     CDS             24931..26970
                     /locus_tag="VGR29_08590"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEV2073682.1"
                     /translation="MPPKSQDDHQDNTVSRRTYSAKPGDSGENAKPSPSEGGGGDRTD
                     FGAFVSRSVRSSADRFKGVAQDADAGGPTEQQGTTPLPRSSPRRERPSRYWRDSLREN
                     TGEDVTAAGPSNRRFVGRPGDEGGRVPSAGDGGDDGGNGAWWQGLTGSGAGNGPGRSL
                     LALVIVGVLVLLLLIFLLTRLLGGDDQQQVDEPVNTPVPTQEIVPGVDIGGTPAGPTP
                     TEGSVEEPTETSEIRRGGDNQLGGEDQEGTPGAQVEEDGDVAESPLARECSGQCLIRV
                     QADNLSRLMEETGNRPSFAGDEIAWVVATPEQVSLLDADAEIELVQDERETLDLYVVT
                     VPEGEDPALVNGYGDIIDESGPYRLIEFDEIPARVATLANSGYNVTKVAPAPPTEEDG
                     NDELPPLSDASADSLMGQVSSSNITEIIADLQRSGAPDGSNLGTRYYTYPGNQIAADY
                     LYRQLESYGLKVWYEDFLTPEGILLVNVVAEVPGEDDSSVYAVMSHFDSINNSDPSIA
                     PGADDNSSGMAVNLEIARILANYELEHPVRFIFVNAEEVGILGANVWARTANTEGVEV
                     KGVFNIDSVGAERQGRYMIINSDAGSRWMKNLLIETNDAYGLGQIIQSEESTEIVADD
                     NMVRDQGIDAVMIARELYGWTPIHHSVDDVAGKVSIDNVQSMTYLVLLSLVQLVQ"
     gene            complement(27069..28205)
                     /locus_tag="VGR29_08595"
     CDS             complement(27069..28205)
                     /locus_tag="VGR29_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEV2073683.1"
                     /translation="MTLFPHQNPDQNNSDDELIENFWAVIPAGGSGTRLWPLSRSARP
                     KFLLPLLGDESLLQQTFARLKRLTAPSRIMVVCGPAHVAAIARQLPDLPTANIIVEPS
                     PNGTGPALALASALIQRQNPRAVMGSFAADHGVIDVDAFCRAVRTSIAAAETGDLVTV
                     GLQPTRPETGYGYIERTDDVRLETVTGTAYRAERFVEKPPFELAQRYVESGKFLWNAA
                     MFVWRVDALTAELRRLQPEIHAGVRKIAAAWESRDQEQVTARVWATLPNVTIDNGVME
                     NASRVAVVPAEMGWSDVGDWHGLGELIDHDDLGNSVRGDLVQIDTTNSVVWSESNRMV
                     ATVGMDNVIVVDTEDALLVIDRSRSQEVRKIVDLLKAATHAKLT"
     gene            28363..>28761
                     /locus_tag="VGR29_08600"
     CDS             28363..>28761
                     /locus_tag="VGR29_08600"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEV2073684.1"
                     /translation="MVPHLDPKPWGGRKLSNYGLELPPDERIGEALVTSGEAIVSAGD
                     GKGLNLQEIVDRDPPGRLGDRAHEAVGGRNIFPLLVKLIDAAENLSIQVHPDDDGARP
                     LDRLGKTEAWYVLGATPGGKLYLGLADDVGL"
BASE COUNT         5620 a         8344 c         9249 g         5548 t
ORIGIN      
        1 ggcaagatcg tgcagctcgg gacgccaatg gaggtgtacg aacgacctgc aaccgagttc
       61 gttggcggat tcctgggcag tccaccgatg aacttcctca aagtccagct tgagaacagc
      121 gatggtcaac cccgggccgc cctggggggt catagccttc ctgttccacg cgccctcgcc
      181 gagcgtggag agcgggatct cctgatgggg attcgcgccg agaatatcga ggcgacaccc
      241 gtgaacggcc agaccgaagc cgatgcgctg gtttcccgaa cccttgtcgt ggagctgctg
      301 gggtcacagc ttctggtgac aggtcaggta ggagatcagc caatcaaggt gcttgtgcgc
      361 gccgacgcac cgatcaaacc cggtgatgat ctgcagctgc gcccggaaga agacaaggtg
      421 cgttggttcg acacgacgac ccgccaggag atcgtcctct catagaggtt ccggagtgtt
      481 gtgaatgagg atcaaagaag gcgagcctcg gggccgcttg cctctctgtg cagagggaac
      541 gacgtagaat gatcggcact gtgcatagtt tgggtcgagc cgtgctcggc ctcctatctc
      601 tggaaggttt gtgtgacctc atccctgttc aacggcaatg tccggggtaa cccataccgg
      661 attcgtgatc cccacaactc aatcctcaca gagatctggc ttgtctcggc gatcgtttcc
      721 gtactcctga ttcgcctcta tctctatctc accggatatc cacaagtagg aggtgagacg
      781 ctgcacatcg cccacatgct ctggggtggg ctgggcatgg tggtcagctt cgctctggtg
      841 atcctgttcg cgcatcccgt atggaagcca atcgctgcgc ttgttggtgg agtcggcttt
      901 ggtgcgttca ttgatgaact tggcaagttc atcacaaagg ataatgacta cttctacgaa
      961 ccgacagtcg cactgatgta cggcattttc atcatccttt tctttctggc gcggtacttt
     1021 gatcggaagc gagagccgac ggaagccgat cacctctatc tagcggttca ggggattcaa
     1081 tggcaagcga tcggcaagtt ggacagacac cgccaggaag cagcgctcgg tcatctggag
     1141 gcaagcggga ataggtcggc gcttgccgga catctccgcc agatacttca gaatgccgaa
     1201 atcgcagagg acgcagggcg atcccggatt gtggccctgc gcgagaaggg cgctgaactt
     1261 tactggagtg tcgtcggtca taagtggatg cggcgcgtga ttgtaggact ctttatcatc
     1321 cgcgcattgc agatcctcgg cgccgtgatc atcggtatgg tgagcgatac cttcatcttt
     1381 acagatggtt tgtctatctc tgaatggttg gccatggcag cgggcggggt gagtggcggg
     1441 ctcgcactct atggcgttgt ccggatcttc cagcgtcgca gagttgatgc gatccaggcg
     1501 cttgctggtt cagcactggt ctccctgctg ttcgggcagt tctttgcgtt tgcctccgtg
     1561 cagttcgtcg cgcttggagg gctggtgcta gatcttggcg cgcttggtgc gctccggttg
     1621 gccctcgccg ccgaaagcga gcgtcatgag cgagaagatg gagaagctga ggatgatccc
     1681 cggcagggtc tggggcagtt cctgtagggc ttgttggacg acgagcggga atgcgcagtg
     1741 aaccaatcag cgatgccccc ggtcgatgta atggatctct ttcctgaggt cagggaaggc
     1801 ggtgtcagga tcctgcagag cctctcggag caggaatgga ggctgccgac tgcgtgtgcc
     1861 ggctggagcg tgcatgacgt cgcacttcac atcgggggcg gattgctggc gaacgtctcc
     1921 cgacgacggg accagcatgt ggggaacttc cgggagttcg agccccctaa caacgcgacg
     1981 ctggatgagc atgagcgact catccacacg ctcaacggct ggaatgagat gtgggtactg
     2041 gccggtcgcc ggatcagccc ggccttgctc atcgagatca tcgattcggc aggatgccag
     2101 ctggaagagt atttcaggag gctggatttc agggtgcttg gcgatcctgt gggttgggcg
     2161 ggcctgcaac cggcaccggt gtggctcgat gtcgcgcgcg agtacaccga gctgtggacg
     2221 catctcgcac agatccggga ggccacagga cgcgccgtcg tggatgcgcc acatctcctt
     2281 gcaccggtcc tggcgacttt tgcccatggc atccctcacg ccttgaggtc gatcgacaga
     2341 ccgatgggag cgacactcac tgtggtcgtt catggagagg ctggtgggca gtggtgggtg
     2401 aagcgcgcgg catcaggctg ggaactgcat ggagaggtga cgggatcggc cgatgcagtt
     2461 attgacctcg accaagtcac ggcgtggcgc ctggcgacga aggggatgag ccgcgacgac
     2521 gccagacgcg aagtcaacat caagggcgat atcgagctgg gtgagggctt cctcaatctt
     2581 gtggcgatcc tggcgtagtt tgcccagata cttctacact ttcggatcgt gcacaccgac
     2641 caggtagtcc cagatccagc gcgaggcacg agccatccga tcaaccgcct ccgcggtgtc
     2701 gtcctgaccc ttgctcatga aggcgacggc gtagttcacc ttcggggagt aggcaatacc
     2761 gacatcgttc ctgatgccgc gcaagcttcc ggtcttgtgg gcggtctcga tgtgctcgtc
     2821 ctgaggcagg cgtgccggga tgatggtgtt gaaattctgg tccttcagga ttcggatcac
     2881 accttcctgg ctctcgcgcg tgagcccgtg tccctcgtgg atctgcacca gcaaccgaac
     2941 catatccgcg ggggagctga catcgttccg ctcatcggcc gcgtatccta cgttgttaac
     3001 cgcgggtgtg tagtccttga gatactcctt aaggaagtgg taatcgacct tgggatcgtc
     3061 ccattcgaca cccgcgagtg tgcagaagag ctgattgata ttcattggaa tgtgtgtctg
     3121 gtgaagcccg aggttggtga gcgtccgggc catgttctcc ttgccgatac gcgcgtagat
     3181 catgtcggtc gcccaattgt cggagacgat gatcatgagc tcggcgaggt cgcggatagt
     3241 cggctgcagg ccctcgtcga gatcctgcag gacgccggag cccggtacgc gatcgctgtg
     3301 acggagggtg acgcgctcgg tcaggtcgat cacgccttca tcggcctgcc ggtaaagctc
     3361 gtagagaagc ggaaccttca gtgtcgacgc ggtagggaag atctccgtat ggttgtaggt
     3421 cagctcccgg ccgctcacaa attcatggac catgacaccg actgtcccac ccaaccggga
     3481 gaaatggctg ttcatcactt cagtgaaagt cgcctgggcc tcatgtcgcg gagtgcttgc
     3541 ggtcacgtct gcggttaccc ttctgttacc ctcgttgttc caaaatccat ccgcgcgatg
     3601 ctagcaatgc catatcatgg cgtcaacggg gcgaggtgac ccgacgacgc agtctgaagc
     3661 ggcatctgta ttgcatcggt agtggacagc gatgatgcaa gcgatccgaa gggagggcgg
     3721 tcatgggcaa cgaatcgacg ctgtacaaat ggctgaagat cggtggaggc atcggtgccg
     3781 cgctaggggt tagggaggcg atcggacggg cgcgggaggt cgatctctcc gggcaggtgg
     3841 cgctggtcac cggatcgtcc agtgggctcg gtctgcttat ggcgcgggaa ttggccgggg
     3901 agggctgccg gcttgtgatc tgcgcccgca gtgagcccga gctggagcag gccggacggg
     3961 atctggagtc gcgcggtgcc gaggtgctgg cggtgcgttg cgatgtcgcc gacaaggacg
     4021 atgtccagcg catgatcgac gcggcggtcg acgccttcgg cgggatcgat ctactgatct
     4081 gcaacgccgg tgtgatccag gtcgggcagt tccagagcat ggaactgaag gatttccatg
     4141 aggcgatgga catcatgtac ttcggtacgc tttatcccat cctggccgcg ttgccgcgca
     4201 tgcgagcaaa ggggagggga cgcattgccc tcgtcacctc tattggcggc aagatcagcg
     4261 tgccttatct gctgccatac aatgccgcga agttcgccgc ggttgggctg ggcgaggggc
     4321 tccgggcaga gctcggcgac gagggcatta tcgtcacgac gatcatcccg ggactcatgc
     4381 gcaccggctc ctacctgaac gcgaagttca gtggcgaggt ctccggccgg gaatccgcct
     4441 accggctctt ctccgcgctg tcgagcgtgc cgctgctgac cgggaacgcc gaaagtgcgg
     4501 cccgtgccta tatccgggcg atcaagcggg gcgatgccta cgtcatgtac ccgccccagt
     4561 acaacttgat cgcccgcatc catggtgtcg ccccggccac gacgatggct gcgatggggc
     4621 tggccgaccg gctgatgccc aagacggggg gcgacaccgg aacggtgcca ggggcgtcga
     4681 tcgattcccg gcttggatcg gggggcatct ggcggacgct gaccacactc ggccgcaagg
     4741 cagcgagcag gatgcaaccg gagcccggcc ctccggactc cggctcgtag gccgagcgag
     4801 cgctactccc cttgctgctt cagagcctca cgcacgagcg gtacaacctt cgtcccgagc
     4861 agttcgatcg atctcagcac cttattgtgc ggcatcgtgc cgacgctgat ctggagcagg
     4921 aagcgctggt ggttgaagag ccgatgctgg tagaggattt tttcggcgat atcctccggg
     4981 ctgccggcca ccagggcgcc gccaagaaag gcacctgcgg cgaactgctc ccggctgagc
     5041 tcgccccagc ctcgctcgcg gccgatccga tccatcatga ccttgtgggc ggggaaactg
     5101 tcggcgatcg cctgctgcat agactccgcg acgtagccat gggagttgat cgagactggt
     5161 acggtgagcg ggtcatgacc gccgcgtcgc agcgcctcgc ggtagaggtt gacgagcggc
     5221 acgaacttcc gggtctcgcc gccgatgatc gcaaccgcca ttggcacacc gagcgttccg
     5281 gcccggaccg ccgactgcgg agtgccgccg acggcgatcc agacggggag tgggtcctgc
     5341 accgggcgcg gatagacacc ctgcccggtc aatgcctccc gtgtgcggcc cgaccaaacg
     5401 acatgctcct cctctcggat cctgagcagc aggtccagct tctccgagaa gagctcgttg
     5461 tagtcgttga ggtcatagcc gaagaggggg aaggactcga tgaacgatcc gcgcccgacc
     5521 atgatctcgg cgcggccgcc ggagagaaga tccagcgtgc tgaactgttg gaagacacgc
     5581 acaggatcgt cagagctcaa cacactgacg gcgctggtca gccggatcct cttcgtgcgt
     5641 gtcgccgccg cggcgaggac caccgccggc gccgaggatg cgtagtcggg gcgatgatgc
     5701 tcgccgagcc cgtagacatc gagaccaacc tggtcggcga gctcaatctc ttcgatgagg
     5761 ttccgcagtc gctcctgcgg agtgatcata gtgccggtgg acgagtcttc cgtcacatcg
     5821 gcgaaactgt agattccgat ctccatgcgc gtcgtctgtc cttgtctgaa ccagtatcgt
     5881 atcgccccaa gcttcatgct tacatatggt aagtagtata gactatcggg catcattggg
     5941 tgcgacgtgg caggatcggt gcaacactgt gcaactcgag agtcgcacgt actgggccca
     6001 ccgcgaatcc gccaccaaca ggaacacgac atgagcccca agaatccaga acgctccact
     6061 ttgcgccaga tacgaaatgc tctccaggtc ctccacaagg cactgctgga agccgagcgg
     6121 cttgagtacg agcgctcgtt tggtcgtatc cagagcgatt tctacctgtt gacgctggca
     6181 gcggaggatc cccagttcgc ctggctgcgg gcgctgacaa acctgatgct tgaggtcgat
     6241 atgcacctgt caggcgaaac gcaggtgtca ccggaagacc tgaggctcac gggcgaccgg
     6301 gtgcgagcgt tgctgatccc cgacgacgcg ccgaccccgt tccaggcgcg ataccagcac
     6361 gcgatgcagc agcacccggc cgtcatcatg gcccatagca gggcgatccg gtcgcttcca
     6421 ccggcctccc ggacccccct gctcaaggga ggcgcgccga cggacaccca gcactttggc
     6481 gacctgcggg tgcagctgca tcatccgggg gagctggttc cgggtcatgg cgaccacggg
     6541 tatgggccgt tggcggtcgt ggcggaatcc tccatggcac ctggctgggt ggtgccgatg
     6601 cacctgcacc ggaacgacga gatcgtgtcc tgggtgccgg agggtgtgat gcgccacgac
     6661 gacctggcgc acgggaagct ggtgacggat ggcgatcacc tgctcgtgat gaatgccggc
     6721 aggcgattct cccatgcgga gcggacgctg gagaccgact cgtcgctgcg gatgctgcag
     6781 atcttcgtcc ggccccgtgc ggtcgatctg gagccgaggc tccagcatgg ggcactgccc
     6841 gtcgccgagg agaacgcctg gaggctcatc gtcgggccgg agggggagga tgcgctgttc
     6901 agcgtgcgca acgaggtcca gatccttgat gctcgaatgg ttcagggtgt ttacatttcc
     6961 ctgccgcagc gggagggctg gctcacctgg ctcttcgtct atcgcggggc ggtggagatt
     7021 gacggcgtgc ggttagggca gtcggagagc gtgctggtgg ggtcggctgg gccagtcgag
     7081 atcggggcag ttgaggagac gctgctcatc gtcttcgtca tcaatcttac ggccaagatc
     7141 acccggtcgg gaacggtccg tagatagagg atgtccaccg gttgccgccc gatttcgtgg
     7201 agcgtccctt caattattcc gtcacctcgg cttaggatgc gccgcgaggc gtggagtggg
     7261 gctccaggta gatgttcctg accgccgggg cggcttcgtg aatccgggca cttatgcggc
     7321 ggagtaggcg ctcaatctcg tccgttgtga ggcgatcctc gatggtgatc tcgccggtga
     7381 cgaggatgct gtccaggcca agctgcatcg tcaacagatt cacaacgtgg gtgacttcgg
     7441 ggaactcctc aatggcgccc cggatcgctt tcctcgcacg cggcgatgcc gatgagccca
     7501 ggagcaagtt ccgcgtttcc ttgccgagca tgatggcaac cgccaccaga atgataccga
     7561 tcaggattga ggcaatgccg tcgtagatgg cgttgcctgt gatctgatgc aggaacaggc
     7621 ctcctgctgc gatgaccaca ccggtggtcg ccgcgaagtc ttcataaagg gcggtcttga
     7681 gggtgacatc cgtcgtctcg cgcaggtagt cacgcgttcc catcccgacg gcttcggcct
     7741 cggactgggc agtccggagg gcgaggatca gcacgatgag atcgaagaca aaggcgatcc
     7801 ccagcaccag gtagctcggc cagtaggctc cgtgatgctc gccaccggcc atgagcttct
     7861 cgataccgtg atagatggag aaaaacgcgc cggcgacgaa gatgaagatg gctgcgagaa
     7921 acgcccagaa aaaccgctcc ttgccatggc cgtaggggtg ctcctcgtct gcggggttgt
     7981 cgctgagggt gatactgacg agcaggaaga cctgattcac cgtgtcggcg gcagagtgag
     8041 ccgcctcggc cagcatggcg gcgctgcccg tgagcagccc gaccgccaat ttcccgctag
     8101 cgatcgcagc gttggcccca agggctgcca gcacgacctt cttgctaccc tttgccacgg
     8161 agccccgctt tctctatttc ggaatttctc gtgcatgtcg ccggcataga gcgcggcagt
     8221 gcacatttcg cctgcaccga tgcccgactg gtcctgcatc ttcaagggac gctcgttgcg
     8281 gctcgcaccg caagccgggg tccacgccct cttcaagaca agtcagtaag atatgtttgt
     8341 tgacccctcc agcgcagtgc gagccggaaa tgatgagcgg tcattctaca cggggtattc
     8401 tcagggcgaa atgggaaggc acatatggta catctcttgc atggcattgg gcatacatca
     8461 tgctgacggc tctcatcccc tgcgcagggt gtggaggacc gtcggggaca cggctgcatt
     8521 cgcgggccgg aatcaagaat tgaggcagca atgaaagaga agtcgttctt cgaacgcgta
     8581 gttatgctcc tgggagtcct cctgtcactc cttgtgggcg tggcaaaatt gttgttctcc
     8641 ctgttcaacg gcttgcgcaa ggtcttcacg agcgagcaag ggcccggcgc cggaaccgac
     8701 atctcgaggg tgggcgacca ggcgaagcag tttgtgagtg gtgcggccga aaagggtcag
     8761 gggctcgtcg aagatgtgaa gcagcgcgcc ggcgacctgc tgagccgtga aagtgagagt
     8821 gagagcgaag gccaggatgg gccggtgacg gagccggatg tcacctccgg tgaggacacg
     8881 gtcgtcgccg ggttcggcga gccgaacgag gaactgagcg agcatcagca cgaggatatc
     8941 ggtggcgtca ctaacggcgt ggatgtcaac acctccgccc agtcgggcgg gtttgtcacc
     9001 tcccgcgggt cggacgtcag cgaccagacc gtttccgccg ccgaaccatc ggactatggc
     9061 tccggcatca acgaaggcgt gccgggcggc ctttatgagg aagacctcaa tcgtcaggac
     9121 gagccgcgtc tcaatgaggg gatcccgaac ctgggcgagc ccgtcggcgt cgagggggag
     9181 gacttcggga cctacgagga ggaaatggtc gaggaggtcg tggcagaggg cgacgaggac
     9241 gatgataccg acaacctttt cgccagtaac cagcccttca ttgtcagcga cgttgccggt
     9301 caggccgata tcccggccgc cagccggggc ttgcgcgagc ccggtgaaga gatcggcgat
     9361 gagcttgata tcgaggatat ctccgatacc gacaatatcg atccctatga ggacttcgcc
     9421 ccctccggcg cgggaggaga ggtggatctc gaaaacgacg agtcaccttc atgggatgag
     9481 tcagagatga cgatcgttga atctccggac gatcccgtgt cggccgacga aattggcgcg
     9541 gaggacgacg atacaccgac cctcggactg cgcgattcgg agtccctcac tatcgccgga
     9601 acgaacgacg atgtatccgg tgcaccgatg ccggagttcc gcggggtgga gacgcgcgac
     9661 gacgatcatc ttggcgtgac cggcgatttt gatcaggcgg atgcgaccga gacgatcgtg
     9721 gctgcccccg gcgataccga caccgtgcac tcgacgcatg ctgatccccg ggaggacttc
     9781 gtcaccggtg acgcagcaga cctggcgatc agtgatgtcg cgggtgacga agactttgac
     9841 agcgctccaa cgggtggtga gtacggcgaa acggatgcct tcggcatgcc tgagacgacc
     9901 gatgtctcta ccggtgacgt aggaagtgat gatgttgtcg ccagcgacgc ctttgaccgt
     9961 ttcattaccg gccatgcggc ggaagtgccg gacgagttgt cgaccgatga cgaccatctc
    10021 ggaatctcgg gcaacttcgg tgaggccgac cggacagaga cgattcccgc tgctcctgcg
    10081 gacccggaga ctgcggctga ggaggaagag ccgcagcagg agacattcgt cacaggcgag
    10141 gatggtgacg ccgataccgg tagctcgggc gagggaggcg cggatccgtt accttctgga
    10201 gccacgggcc agggatccgg tccatctgcc gcctggagca cgtcggccgg cttcctgagc
    10261 acggaactca cccagccatc agacgtcaac ataccgggcg gcgatgaggg tggcagcgga
    10321 gggtcggagg aacttccgac tggtgaggct ggtggcggtg gcgcaggcca gggcgatgga
    10381 gccatggatg tggatgttgc ctccagcggt gctgccggcg agtctcctgc cgcggcgatc
    10441 aaccagcatc tcgacgtagt gcaggataac cttgaggagt cgcttgcgga cgatgcagcc
    10501 gacgtggcct cagcgtcgac cggcgatctc ggcactacca cgggcccgga tgtttcggcc
    10561 actgcgggca cggcgcgcca gggtggcgat acaacgccgg attctggttc ctcgaggaag
    10621 agccggaagc agcgggccca ggatagtgac tgggttccga atggggcagt gaagggcgac
    10681 ggtagccata cctgcccggc ggagtacccc atcaagggca atgccaactc acgaatctac
    10741 catcgcccgg aggattcatc gtacgactcg acgatcgcgg agtattgctt tgccagtgaa
    10801 caggatgcta cggcagcagg cttccgcgca cggaagggat agcttcccta aagtacggaa
    10861 catcgcgaaa ccccttctgg cgagagccag aaggggtttt agcttgtcag gatagacgtg
    10921 ggtaaggtta gccgttgctg agccgctcgt cccgttcttc ctcagccacc ccgtcggacg
    10981 cgggcttgga gcgcccggtc acgttggact ccatatcgct gccattggga tcgagctcag
    11041 ggcccagctc gtcctcgggc tcgccgtccg tcgcgatcgt gggagggaac tgctcgacgg
    11101 gctgaaactc gccttcaagc ggctcagtgg ttctgtcgac atcattcgac gtgttgcggt
    11161 cgctcatgat tcctggtcct ttcgcatctt cgcatgaatt catgtggagc tcgctggtct
    11221 tcttgtgtca tggggtatgc aagagacgtt ccagcttcgg tccaggctca ttggacatgg
    11281 agtttggagg cggatcctgc agggagcgcc cggatacctc acatcgtatt gccgaagtgc
    11341 gccagctccc aatgccattt attcggccgg ttggcggtcg gatttccact ggacaaagcc
    11401 tgtggcgtca acgacatggt cgacgatctg actcacgaga gcaaggtatt ccaccgttga
    11461 acccgtgcaa accgcattgc gtacctggag gccaaggggt ggatcgagca cgtcgagctc
    11521 ggcgaaaagg tccttcgttc ctggtagcca gcggccctcc tgaaggaatg caaggcgcgc
    11581 ggcactgatg accacgtcga cgagcatggc cctcgcgggc tcgggatctc gctctatgat
    11641 gtcgatggca tcctcgaacg acgtggcaat agcgtagcgt tgccagacga ggtcctgcgg
    11701 gattacctac ggaccgcccg ccaggttctc ccgggacttc ggctcggagc gtctccatga
    11761 tccctcaggt gtcttggatg acggtgccgg tcgccatcat gtgcgccatg acggcacgtc
    11821 cggacgctgc ttcctgttga tacgagcgcc ggattccctt gggcggattg acgaagattt
    11881 cggccggcac gccgtcgagg aacctctgca aacgctgacg ccatgggggt agtggacgac
    11941 gtcaatgtcc cgatcgttgg caggatccgg gttgccgctc acaacgattc catccgggtt
    12001 gaactgcatc tccatgaacc ggagtgcagc atcggaggcg tggcgatacc gtcccgcaag
    12061 cgcctctcga aacggctcct cgctgcactc catcaggcat cgagaagtgc tggtgcaatc
    12121 gcctcgagtg ttgcctcatc cacgtgagag atcacggcga tccggtcacc gccagctcga
    12181 ccccacatca cactcagcgc gttgccgaca gcatcgctgc cgaagatctc gtagtgagcg
    12241 aagggaatcg cgttgccggg aagaaggttc cacccatgga gggtgaaggg tggggccgtc
    12301 tcgtatgtct cgatctcgcc ccggatagcc tcgggaatgt tcgtctccgg ttttagcgag
    12361 agaagttgca catagccgac cgggtaatgc tcggaagatt cgtatcgcag ggacacttcg
    12421 ccgccggccc aatccggaag ccgggtttca tggggctcaa ggtgcgcgag tgttcccttc
    12481 atggggccgt cccctaggga tttcggcagt cggttgagca gatcgtcgct gaggacggcg
    12541 ttctcgagcc cgtagggcac tgtgccttcc tgtgggacat tgagtgtctg ggtgaagcac
    12601 tggatctgct gatcaaccca ggcgcggtcc cgtccgaaga accacttccg ggtattgtcg
    12661 tggtggacaa gacggacgac atcctgctcg acactgccat ctccgtattc ctgccggaag
    12721 gagacttccg ccgtgccagg ataggtgacg ccgttcacgc cccaggtcca gttcttgtag
    12781 gtgacgccgg tggcaaccgc cggtttgggg tcccggtcct ggaacacctc gcggaaatag
    12841 cggatgacga cgtggtgcgg cacaatcgcc tgggcatcgg gatgcatcag cgcgtagaac
    12901 tcgtagaggg cggggatgtg cgagatgacc tccaattttg agatctcttc tgccgcaacc
    12961 atcggatccc cgtggacatc ccgttcctgc cccgagagca tgtggggaag gggggaacca
    13021 cacacgatca ccgctgtggc tgccacgaaa gctcgtcgcg agagtggagc cgatgccatg
    13081 agtattctcc ctgcacgccg ggttggctcc gtatcccctg ggacttcagg tcgcggtggc
    13141 gtccacgtta cctgatcgtc gcgttgtcct ccacagtgtt gggccgataa gagcaagcag
    13201 ggcgatcgcg aagatcgtag cggcaatggg cctggtgacg aagacggagt aatcgccctg
    13261 gctgatctgc aatgcccgac ggaactgcgt ctcagcctcc ggaccgagaa tgaccgcgac
    13321 gaccgcgggg ccgaccggga tgtcaaagcg ccgcatgacg tagccgacga caccaatgat
    13381 gtagagcacg atgaggccgg tgaccgactg gttctgcgcc caggtgccga gtccggcgaa
    13441 gacgaggatg ccgctataga gccaggggcg aggaatcgag agcagcttga cccataatcc
    13501 gaccagtggc agattgagca ccagcaacat gaggttgccg atatagaggc tggcgatgag
    13561 accccagacg agctccgggt tgcggtcaaa gagctgtggt ccagggttca ggttgtatcg
    13621 ctggaaggct gcaagcatga tcgccgcggt ggccgatgtc gggagcccaa gcgtgagcag
    13681 tggcacgagt acccccgcgg cggaggcgtt gttcgcagcc tcggggccag caaccccttc
    13741 gatggctccc tttccaaact cctccgggtg cttcgacaag cgcttttcaa tggagtagga
    13801 gaggaatgtc ggaatctcgg ctccgcccgc cggcagggca ccgatgggga agcccaaccc
    13861 tgctcctcga agccacggcc tccacgaccg cttccagtcc tcctttgtca tccatttgga
    13921 cccggatatc tcctgggttt tctgcttctc gcgtctcatg tgcgatgcca cccagagcgt
    13981 ctctccgacg gcaaagagac ccactgcgac ggtgaccaca tcgacgccgt tcaggaggtg
    14041 gggattgccg aaggtgaatc gtggttggcc cgtcagggcg tcgatcccga tgaggccgat
    14101 ggcgagcccc aggaagaggc tgaccaggcc gcgcaccatc gacgagccga gcagggtagc
    14161 aacggtggtg aatgcaagga ccatcagggc caggtattcg gcggggccga accggagggc
    14221 gatccgcgcc atggccggcg cgacaagggt gaggccaatc gtcgccaggg tgcccgccac
    14281 gaaggagccg atggcggccg tcgcaagagc agcggcacca cgccccttcg ccgccatcct
    14341 gttgccttca agggcggtga tgatggatgc gctttcaccg ggagtattca gcaggatcga
    14401 tgttgtcgat ccgccataca tcccgccgta gtagatgccg gcgaacatga taaatgcggg
    14461 gatgggatcg agggcatagg tcacgggaag caggagcgcc accgtgagcg cggggccgat
    14521 gcccggcagc acaccgacga atgtgccaag aaaaacgccg accagcgccc agagcaggtt
    14581 ctggggcgtg agggcgatgc tgaagccgtc cattagagaa gctatcgagt ccataagctg
    14641 tccttcagca tctcgtgagt gcatcacttg gatggtgtga cgcgttctcc gacgacaacg
    14701 gcgcgtatat cagctttggt agtactgctg caagatcagc tcgtgaggac tccgggctgc
    14761 tacctgattg actctaagat atgaatcgtc catgggagct gttcctccca tcgggaagca
    14821 atgacaagca actctccccg tgctgtcatt cgagtacgaa ttccagaatg ccagctggaa
    14881 ggcggacgcc aagccaggtg tcaaagagga gaaagaccgc cgacgccagg atcaggccga
    14941 tcagtacgtt tgcaataatc ctgcggctgc ccatggcggt tgatgcgatg gcgaagagga
    15001 tggcactggc gaggataaac ccggcggggc ggatcaggag cgcgaagatc gccaatccaa
    15061 tgccgatgat cgccagtacg ccccaatccg tcggcgcatc gatgtcgacg tcttcggcgt
    15121 cttcaccggc cgtcagcacg tggggcttac ggatgatatc gatcgcatac caaacaccga
    15181 ccacaatcag tccgccacca acaagatttg ggatggcacg agggctgacc tgggacatcg
    15241 agcgaatgac gcggatgtct tgagtctgca ccagtatgat gatgccggcg atgacgagca
    15301 ccacggggat gaggatctcg ctccaccgcg ccaggccagt gggaacgtcg acgttgtcat
    15361 ctgcaggcga tgggcccggg cctgtatcgg gttcgatcac catgggtggg gctccaggcc
    15421 gatgccgggt ttccttgttg agaaacccgg catcggcacg attgatcaga gaggctattc
    15481 gccgccgatc agctggagat cggtcaggat cgtgttgacc cgctcgatct cgctctggat
    15541 gaaggcgacg tattcctcgc cacccagata gaaaccggtc cagcccatgc tccggagcgt
    15601 gttctgccac gtctcggaat tgaccgccgc ctcgatcgcc tggttcaggc aggcctgatc
    15661 ctcttcggag atttcgggcg gagccacgat accgcgccag ttggcgagct cgatgtcgat
    15721 gccggattcc tggagggtcg ggatgtcagc gccgaatgct tccggcgttg cgccgccggc
    15781 aacgggggtg gcatcgccgc tgtcagcgcg gccggagagg gccagggccc ggaggtcacc
    15841 gctctcgatc tgggcctgcc actcgccgag tccggaaaca cctgccgtcg tctgcccacc
    15901 gagaagggcg gagagggcct cgccgccgcc agagaacggg acgtagttga tgagggtcgg
    15961 gtcaatgcct tgcgattgcg caagcaggcc gaccagcagg tgatcggtgc caccggcgga
    16021 accacccgcc caactgacgg cacccggatc ctcctggaag gccgccatca ggtcgtcgat
    16081 cgtctcgtac ggagaatccg ccggaacgac gatgacttcg aattccgtgg tcagtcgtgc
    16141 gaggggggtg acctgctcga gtgttaccgg agattcgttg agcccgatgc cgccgatcat
    16201 cacgagcccc atgaccatcg ccatgtgacc atctcccgca tgagtgttca ccaactcggc
    16261 caggccaatc gtgccgcccg cgccctcgac gttgaagacc tcgacgttct caacgatgcc
    16321 ttcctcttgg agggcccgcg caagctcgcg tgccgtggag tcccatccac cgcctggagc
    16381 agcgggcgcc atgatctgga gcgtctcgga actccactcg gtgcaatcga ctgcatcgcc
    16441 ctgggcgaat gccatgcccg gcaccatcgc gccaagggcc agaaccgcgg caatgaccag
    16501 gagccccagg gctcgaagtg gatgccacga cgtttgggat gtggacacag acgttctcct
    16561 tttcccgccg gaggcgggca ttacgacgtc cggtacggga ccggtccgat cgacgctcac
    16621 ccaactcccc cgacggtcac gatgacggtg tgggcggctc ggcccatgag gtccgggtgc
    16681 ctggagttgt ctcgtgtgcg ttgcgcgaag cgtaacaaat ttcagaaaac cgcgcggggc
    16741 tcctggtaat ggttttaggc atgtacgaca aagccacctc tctggagaag aggcggctta
    16801 ggcaatacag cgcaacggtg aaatggatca atcgtcctcg ctggggccct cgacccaatc
    16861 accctcatcg tttttgtgat atttcttctt gaccgcaccc caggcgaccc gatgggctcg
    16921 cgcctcgtcg tgaccgtatt catcccaggc gctattgaac gcttccttgt agatttcctc
    16981 agcatgcttt gggaggttgt ctttcaccga ttcgggcagg tcgccccggt tcttgtatgg
    17041 catgggtcgc tcctttctct tgaacaaggc gtgcaggctc catgccgtca ctgcaccgaa
    17101 ttaggcaagc aggattcttg cgccgttgaa gagggcagga tcgacgaagt caccttcgcc
    17161 cgggccaatg gcgccgtcat gggtgcgaag cgtaagccgg tagggtccct ggccgtgcct
    17221 gttcaggtgc tcggcagcct catgggcctt gggctcaacc agcatgatcc actggtcccg
    17281 ctctctgcct accgggaaga ccactcccag gcggtcatcg gagaaggggg ctgtaagctc
    17341 gtgccccgct gtgccgagga tggccgtgta gtcgcgcacg gatgcatcga gatcatgcac
    17401 caggatggtg acgcccgcgg cgccgaggac cccattcggg tggcgcacct cgtccggcgc
    17461 gcgtggcacg cgcagctcac gcggtgtaac gtcctcgatc aggaagggcc agccggtttg
    17521 gccgaccgga cctggaggga cgctcaggag ccaatcgatg cgcttgccgt caggtcgatt
    17581 ccgtcccatc gaaaatggct gcgggtattc gacaccctga tcccggatag ccgcgatttc
    17641 atcctccagt tgatcgccga ggagacagaa atcgacgagc cctccggcgg caaagaggcg
    17701 tggaaaccag cggtgctctc cctcaggagt tgcgctggtg ggagtgatta gctcgatata
    17761 ggcaccatcg gcgaacgcga tcagcgcgtt atgcgtgcgg ccatcgccat gggtgccgcc
    17821 gggcacgacc gtgaatccgg ccctttgcgc attatccata gcggtcttga gatcggcgga
    17881 gatgatgacg aaatggtcga attggcgcgg catgcgggtc tccttgcacg gtacgggcgc
    17941 gagcggtact ccggctttca ctataggggc attgtgcgaa cttgcagata ccctctacca
    18001 ttaccagggg agtgggaggg acaggatcgc cgtcaatcta tcaccatgcc ggaagcggga
    18061 taccgcctcc aagctaccag gaatgctcca tgactcctca caatcgccac ttgcattcga
    18121 cttcgaatct ttcccgccgt catttcctca ccggcgccgc gctgggcgtg gccagcctcg
    18181 cgctgccgca gtcgccacac gctgtttcgg cgcagagcat ggatcctttt accttcatca
    18241 ctgccatgaa gacgaccacc gatgtcggtc aggtcgtcgg ccaggcggcc tccgagatcg
    18301 aacgtgtgag tgccgatgga tggtactggg acgcctacat ccaggttccg gtgaaggagg
    18361 ggcaggactt tcacttcacc tgcgagttcg attctgcctg gatcatcctg aaggcttacg
    18421 gcttcgacct cacacttgag gagcagatgg cgatcgtggg cgtcgataac gcgatcgagc
    18481 cctggtacga ggagacaccc acagggatca tcgtctgggg cggggacatc gatgagcact
    18541 actgcggcca ttacgacacg aatgtgctca cccgcgcccg ctgcaacgcg atgcgcaagg
    18601 tctttgaatc agtcgggctt ggggtgacct tcacgccgga gcgcaggctg attgaggaag
    18661 ccctgctgcg cggtgagccg gtcttcttca aatcgactgt cgattttctt ccatggcggg
    18721 cagccacctg gaaaactccg gacggcgacg attttcccgt tgtgctgacg aacgaccacg
    18781 ccttaacggt gatgggcttc aatgccgatg aggtcatcat ccgggacccg cttggcccga
    18841 ccaccacgaa ctacaagcga ccatggcaat accgggtctc ttggggacgc ttccttgagg
    18901 taatcgccgc gcaggggaac gatgcgattg cgatcgcccc gtctcctcct ctgacctgat
    18961 tgtccgaagt accgaaccga ttagtaccga ctggtgccga tctgcatcgc tgttacgaga
    19021 agagttgagg tgagcgttgt cccggagacc tgcgcggcat tacgacctga tcgtgattgg
    19081 tggtggggcc agtggaagct tggttgcctc cggtgcggtt gatgagggcc tgagtgtcgc
    19141 gctgatcgag gaatggaagc tcgggggcac ctgcctgaat gccggatgtg acccgaccaa
    19201 ggccatggta cgcaccgccg aggtgcttca cctggcaagg acagcgagcc gattcggaat
    19261 ctctgtcccc aaggcccgtc ttgagtggga agcactccgc acgcgggtcg atgacctgat
    19321 cgacgaaatt cgtggcggcg acggaccggc caatgtgcga gcccagggaa tcgacctgta
    19381 caagatgcac ggcaaattcc tctccgagaa cgaggtgctc gccggggata gcctgctcac
    19441 gggtgaccgg gtcctcatct ccacggggca gacagccaga gtgccccaag tcactgggct
    19501 tgagcaaacg ggctacctca ccaatgtcga tgccgtttcc ctcgacgagc taccagagtc
    19561 gctgttgatc atcggcggtg gggctgtggc agttgagttt gcacagatgt tctcgcgtta
    19621 tggtgccgag gtcacgattg ttggaacgca ggaacacgtg ctcccgaagg aagatgagga
    19681 gctctcgggt gaattgacca gaatccttag ggatgaaggt atcaccttgc agatgaggtc
    19741 ccgggcaacg ggtatacggc gatcgccgga tggccgggtg atcctcacct gctcgcgcga
    19801 tgatggtccg cccgtcgaac ttgctgccga tgaggttctg gtcgcgacgg ggcgagtgcc
    19861 gaacatcgcc ggattggacc tcgatgtggc cggtgtccgg tttggagaac ggggcatcct
    19921 ggttgacgcg cagatgagga cgaatgttcc ccacatcttc tccgtcggcg acgtcacggg
    19981 catttacccc ttcacccatg tggctgacta ccaggcccgg atcgcgttgc acaatattgt
    20041 gaacgaggac ggggcaatga aagcggatta ccgggtcgtc ccgtgggtga cgttcagcga
    20101 tcccgagctc gcccgtgtag ggctcaccga ggcagaagca cgggcgggag gatattccgt
    20161 cgtgacatcc actgttccct tcgctgaaat gacccgggct atgacctcgg atcagcgcga
    20221 gggtctcgtc aagctggttg tcgatcgtgc tacgcaccgg gtgctgggtg gtcacatttt
    20281 gggagccgga gcaggagagc tgatcggcga gatcgcgatt gtcatgcagt atcgactgcc
    20341 ggtttccgcc ctcagtgaca cgattcatcc ctatccaaca atgtccgagg cgatcttcaa
    20401 agcggcccac gacctgatgc agggcgcgct tgcgggaacc tcgccggtga acgtcgggta
    20461 ggagcaggcc gcgttttatc cctgctgctg cgtcgtctcc atcgcgattc gcaaccctat
    20521 cgtcagttca tcggtgcccg gaagatccgt ccatcgatgc agcatccttg cagattgcgc
    20581 tcccccgggt gctgatggca ttggcacccg aatgatctcc gtgatgccgg acgtgaaaac
    20641 tcttgcggcc accacgtgtt gctggcggcc atcgttccac tggagtgtga ttgctgagcc
    20701 cgcggggaat cgctgcacgc tggttgtcac gcgggagggc cagccactgg caacccggtc
    20761 gaccgcctcg tggagcgttt gctcagccgg actgtacgcc ttggggtgga ccttgcccac
    20821 ctgcacacct cccaacaagc ccctctggta tcccggtggg atgtcggtaa gccgggcaat
    20881 gcgggccctc ataccaggat ttgtcaggag ccagtaacca gcggcaagga tgagaatctg
    20941 aaccgcgacg atgatgccgg atagccaagg cacgccgctg tcatacatct gacgccagtc
    21001 gccgttgaga ccggagagca tgtccaggac cggatagacg atgaaggcgt tgaggccgga
    21061 gatcaggaca aactggatca gcagctcatt gaaggccgca cgcatcggtg gcttcttgag
    21121 cagtacgata gccagcccga tgacacagag catcaggttc acgagcgacc cggcagcggc
    21181 aatgatggtt tgctggacat cggagaggcc gaaggggatg taagcgacat atcccgcaaa
    21241 accataaaaa ccgaactcga cgacctccct gccgaagctc cagactgcaa ccgcgtgacc
    21301 cagctcgtgg agcgccacag agatcgggat gaagacgaaa aacgctgcct ggtcgaccag
    21361 atggcggtcg gtcggggtga agtgatggtc ccaggtagcg cgccaactgc ggaccagctg
    21421 ggcgacgaca cggactccga tgaccacgta gatgaggctg atgattgaaa aggcgccgaa
    21481 gtccataggg ctcctggata gatgacgaac acgcgggcga gcagacgccc cgtgtcagtg
    21541 tgcgtgtgcg ggcgccagag tgcaacggag agggcagcct gcgcgtcata acgcctggca
    21601 aaaatcacca agacgtacgc caggtgtact cctattggct ctccagtcac cggtctaaga
    21661 atagctctag gggcggaaac cgaatgccat gcttccactg gcactatcgc ttgggtactg
    21721 gcgacgtggt cattgtctga ggggctgctg ctcgtgctct cggggaacgc tgtagccaga
    21781 agagggtgat cgcgacgaga gtcacgattg tgccgccaaa cgtcattgag gtgatgacac
    21841 tgaaccggtc cgagactatg ccggcgatca acgcaccgat tgggacactg cccatgaaga
    21901 cggtggaata gaccgccatc acgcggccac gaagctcatc gggtgccgtt gtttgcacga
    21961 tcgtattcgc catagccatg gcgttgctgg cggcgaagcc tgagctggcg agcagcaaca
    22021 tggcgacgcc cacgggaagc gggattgagg cggtgacggc aagcgccagg atcacgatgc
    22081 caagcgcaat ggcagcgacg atcatgcgga cgcgactagg tttgttcccg gaaaatgcga
    22141 ttgagagggc accgatcagt gatccggccc ccatggagga aaagagcagt ccgaagatcc
    22201 ctgcatccgc cttgaaactg tcggtcacga ggagcggcag ccagacgtta tacgccatgc
    22261 caaaggtgcc cacgaaggca acgagcgtga ttggccgcat gatatccgcc gtgtttcgaa
    22321 cgaatcggat gccctcgcgc agctgttcgc cagccgatcc ggtggttgcc tgaatgacct
    22381 tcgtgatcct catcatcaac aggccggcga tgaccgccag gtaggaggtg gcattcacgg
    22441 cgaagcagac ggcggggcca aaggccgcga ggatcacccc ggcaacagcc gggccgagga
    22501 ttcgtcccgt gttgaacatc gccgagttga gcgcaatggc gttcatcagc gcttccttgt
    22561 caaccatctc ggagacgaag gcctggcggg cgggcatatc aaaggcgttg gtgaccccga
    22621 agaagccggc gagaagatag acatgccaca actcaacctg tccggtgaag acgagatagg
    22681 agagagccgt cgccaggatg ccgaagatca cctgagtgac caccatcagg tttcgcttcg
    22741 ggatcttgtc ggcaacgacg ccggcaggaa taccaaggag caggaccggc ccgaactgga
    22801 agacattgac cagaccgagt tggaaagcgg atgcgtccag tcggtccacc acgagccagg
    22861 actgggcgag ggattgcatc caggttccag tgacggagat caactggccc gtgaagaaga
    22921 gccggtagtt gcggtatttg agtgcagcaa atcctcgcga gagcccacct gatctggcgt
    22981 cgggagcagt gacgttctcg tctctgccac cagttctgga agattgactg tttttgctcg
    23041 acatgtggtc ccggcgctgt ttgcgcattc tctcaacggc ccgcgatgtt tcacaggcga
    23101 aggcgagtga gagtcggatt ctgcgacctc ccatccctga tgccaccagg ggtgaaaggc
    23161 atcattgggc cgatcgtgct cgacgcctta ctcttcgcat acccaccgcc atccgtcaat
    23221 agatgtgtga aggatgcatt ggggacaacc gggacgcctt tatctcctga agcaattgag
    23281 tagacgcacc cggacaccgg ccgagcttcc tggccgcagg tccgctgcac ccacacatgc
    23341 ctgctactga tcgccctgct tgcgtgcggc gctggcgcga tgcaggagca ccgggcaggt
    23401 ggcgcggcgc atgacttcct cggcgacgct gcccaggaac cagcgcttga tgccggttcg
    23461 gccatgagac gacatgacga tcacatctcc ctcatcggcc gaattgacga tctgctgcgc
    23521 gataggtcca tgcagaacct ctccggtgac ggtcaggccc tcctccgaaa gcttcccgat
    23581 ctgatcctca atataggagc gagcgtcctc gaactcctcc tccgccaatt ccgcaagcat
    23641 cgagtagtcg agtgccatgc ccgttgccgg taccgtctgt gcccggtatg tatcgacgac
    23701 ccgaacgata tggaggggga tattcatcgt cgtggcaagt ttccatgcct gggacaacgc
    23761 ctcctcagcg agggcggagc catcgagcgg gacaataacg cggttataca tatccctttc
    23821 cttccaggtg atttcgaccg ccactctcgc ggccagtatc tgccattttt attatgctcc
    23881 ggtgcctgtt gcagacgggt ggataatcta cttagcccgt tacagatcca tgacactgga
    23941 aatccggaga catcacagcg cacgaggaat aaccatgtcc gacatccatc gacagagtga
    24001 ccaaatgcca cagtcgcaac actgggaggc agcggaaccg atcttgctcg cgaacctgac
    24061 atcaccacag acaggtcaac tccttcaatc catagagatg gtgctgattc cggttggcgc
    24121 gcacgagcag catggccccg cattgccagt gtcaacggac gcgctgacgg cgcaggtgtt
    24181 atgtgcattg acaggagcgt tgctcgctcc tcgggtcgcc gtattgccag tgatcccgtg
    24241 gggagtgtcc tggcatcacc aggggcgacc tggcacggtg acgctccgcg aagagacgct
    24301 tatctcgctc gtcctggatc agattgactc tctgcaccgg cagggcgtaa aacggatcgt
    24361 cctcgtcaat acgcacggag gtaataatcc ggcactggcc atcgccgctg agcgagccaa
    24421 gcgggagctt ggtgtgccgc tggttgtatc tgtgtacgcc tacacgctgc tggccaatgc
    24481 cgcccgggag attctgggcg acgacgcgat cggtcacggt ggaggggatg aagctgcggc
    24541 agtccttgcc atccggccgg atctggtcga tggtgaccgt cttggtcccc agcaactgaa
    24601 ctccgcgatc cagcggcaaa agacgattgt ctcggcggtg ggaggaacat tgccggtaat
    24661 gatgcacaaa ctgacggaga gcggcgcgac cgggaactcc atcaacgcga caacggaagc
    24721 cggacacgcg attctcgggc aggcagcgag tgggcttcga gcggtctgcg aggaattgct
    24781 ggaggctgat cttgctcaac tgtgatcctg gcagatagcg cgtatcgggc gaacgaatat
    24841 ctcgccacgc aatgttccgg tttgctaggc ttggcaatac agggatgtca ttcgtgacca
    24901 gctcccttct gcgtgtgtga ggaactaccg gtgccaccga aatcccagga cgatcatcag
    24961 gacaataccg tttcgcggag gacctattcg gcgaagcccg gcgactcggg ggagaacgcg
    25021 aaaccttccc cctccgaggg gggtggaggc gatcgaacag attttggtgc ttttgtctcc
    25081 cgctctgtgc gctcctccgc tgaccgtttc aagggagtgg cccaggatgc agatgcgggc
    25141 ggtcccacgg aacagcaagg cacgaccccg ctccctcgat cttcaccccg gcgcgagcga
    25201 ccctcccgct actggcgcga cagtcttcgg gaaaatacag gcgaggacgt cactgctgca
    25261 ggtccatcga accggaggtt tgttggacgg ccgggtgatg aaggcgggcg agtaccctct
    25321 gctggtgacg gcggcgatga tggtggcaat ggtgcctggt ggcaggggct gacgggctcc
    25381 ggcgcgggca atgggcccgg caggtcgctg ctggccctgg ttatcgtcgg tgtgctggtc
    25441 ttgttgctcc tgatcttcct gcttacccgc ctgctcgggg gtgatgacca acagcaggtc
    25501 gacgagccag tcaatactcc ggttcccacg caggaaattg tgcccggtgt ggacatcggt
    25561 ggcactccgg cggggccgac accaactgag ggatccgttg aagagccgac agagacgtcg
    25621 gagattcgtc gcggcggtga caaccagctc ggcggcgagg atcaggaagg cacaccgggt
    25681 gcgcaggtgg aagaggacgg tgatgtggcg gagagtcctc tcgcccggga gtgttcgggc
    25741 caatgcctga tacgggtcca ggctgacaat ctgtcaaggc tcatggaaga gacgggcaat
    25801 cgcccgtctt ttgctggaga cgagatcgcc tgggtggttg cgactccaga gcaggtgtcg
    25861 ttgctggatg cagatgcgga aatcgagctc gtacaggacg agcgcgagac gctcgatctc
    25921 tacgtggtga cagtgccgga gggtgaggac cctgcactgg tgaatgggta tggcgacatc
    25981 attgatgagt ctggtccgta ccgtctgatc gagttcgatg agatccccgc ccgggtcgcg
    26041 acgctggcaa acagtggtta caacgtcact aaggtagcgc cggcgccccc cacggaggaa
    26101 gacgggaacg acgaactccc gcccctgtct gacgcgagtg ctgattcact gatggggcag
    26161 gtgtcgagtt ccaatatcac ggagatcatc gccgacctcc aacgatccgg tgcaccggac
    26221 ggatcgaatc tcggcacgcg ctactacacc tatccgggca accagattgc cgccgactat
    26281 ctctatcgac agctggaatc ctatggactc aaggtctggt acgaggactt cctcacaccg
    26341 gaagggattc ttctggttaa tgtggtggct gaagtgcctg gcgaggatga cagttccgtc
    26401 tatgcagtga tgtcgcactt cgactccatc aataatagtg acccttcgat tgcccctgga
    26461 gcagatgaca actcaagcgg tatggcggtg aacctggaga tcgccaggat tctggccaat
    26521 tatgagctgg aacatcccgt ccggttcatt ttcgtcaatg cagaagaggt tggaatcctg
    26581 ggtgccaacg tctgggcgcg gaccgcgaac accgaaggtg ttgaggtcaa aggggtgttc
    26641 aacatcgact ccgttggcgc tgagcgacaa gggcgataca tgatcattaa ttcggatgct
    26701 ggttcgcgat ggatgaaaaa tctccttatc gagaccaatg acgcctatgg gctcgggcag
    26761 attattcaat cggaggagtc gactgagatc gttgcggacg acaacatggt ccgcgatcag
    26821 ggaatcgacg cggtgatgat cgcccgggaa ctttatggat ggacgccgat tcatcacagt
    26881 gtggacgacg tcgccgggaa agtctcgatc gataacgttc agtccatgac ttaccttgtg
    26941 ttgttatcgc tggtgcagtt ggtgcagtag gtcaggttgg atagcgccgg cgtgggcatc
    27001 ttggatatct gcccaatggg ctccgtgcca gctcgatttg ccaccacagg ctccgatagc
    27061 gacccttgtc acgtcagctt ggcatgcgtc gctgccttca ggagatccac gatcttccga
    27121 acctcctgcg atcggctgcg gtcgatgacc agcagggcgt cctcggtatc caccacgatg
    27181 acgttgtcca taccgacggt tgccaccatc ctgttggatt cggaccagac aacactgttc
    27241 gtcgtgtcga tctggaccag gtcaccacgc acagagttgc cgaggtcgtc gtgatcgatc
    27301 agctcaccga gcccatgcca gtccccgaca tccgaccatc ccatctccgc gggaacaaca
    27361 gcgacccggg aggcgttttc cataacaccg ttgtcaatgg tgacgtttgg cagggttgcc
    27421 cagacgcggg cggtcacctg ttcctggtca cgggactccc aggcggcggc aatctttctt
    27481 accccggcat ggatttccgg ttgtagtcga cggagctcgg ccgtcaacgc gtcaaccctc
    27541 catacgaaca tggccgcgtt ccagaggaat ttgcccgatt cgacgtatcg ttgggccagt
    27601 tcgaacggag gcttctccac gaaccgctcc gcgcggtacg cggtgccagt gacagtctcc
    27661 aggcgaacgt cgtcggttcg ctcaatgtaa ccgtagcctg tttccggtcg cgtcggctga
    27721 agaccaaccg tcacgaggtc gcccgtctcg gctgccgcaa tggaggtgcg aaccgcccgg
    27781 cagaatgcat caacgtcaat gaccccgtga tccgctgcaa agctgcccat gacagcgcgg
    27841 ggattttgcc gctggatcag ggccgaagcg agggcaaggg cagggccagt gccgttgggg
    27901 gagggctcca cgatgatgtt ggcggtagga agatccggaa gctgtcgtgc aatcgccgcc
    27961 acgtgtgcgg ggccgcagac gaccatgatc ctggacggtg cagtcaggcg cttcaggcgc
    28021 gcgaatgtct gttgcaacag tgactcgtct ccgagcaacg gcagcaggaa tttgggccgc
    28081 gcggaccggc tcagtggcca caagcgcgta cctgaccccc cagcgggaat gacagcccag
    28141 aagttctcaa tcaattcatc gtcactatta ttctggtcag gattttgatg cgggaacagc
    28201 gtcatgactg agtcagtcct tgcagtggtc tggctggggc tggcatgctc ggaacctcgt
    28261 ccatggttcg atctacgcta gcatgtttac aggaacgatc atgactgatg ctgggtccgg
    28321 gagcaaacaa ttgaaatgac gcatcgcgca agagtctcgc aggtggtacc acatcttgac
    28381 cccaagccct ggggcggcag gaagctgtcg aactacggac tcgagctgcc gccggacgaa
    28441 cgaattggtg aagcactggt cacatccggc gaggcgattg tctcggcggg tgatggcaag
    28501 ggcttgaacc tccaggagat agtcgaccgg gatccgccag gtcggctcgg tgaccgggcc
    28561 cacgaagcgg ttggcggtcg gaatatcttt cctttgctgg tgaagttgat cgacgccgcc
    28621 gagaatcttt ccattcaggt gcatcctgat gatgacggcg ctcgaccctt ggaccggctg
    28681 ggcaagacag aagcctggta tgtgctgggc gccaccccgg gagggaagct ctacctcggt
    28741 ctggcggacg atgtcggtct g
//
LOCUS       DASYWM010000212        13375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_571910, whole genome shotgun sequence.
ACCESSION   DASYWM010000212 DASYWM010000000
VERSION     DASYWM010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13375
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571910"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VGR29_08605"
     CDS             complement(<1..337)
                     /locus_tag="VGR29_08605"
                     /inference="COORDINATES: protein motif:HMM:NF021489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV2073685.1"
                     /translation="MDQGTRDEKIAKGCLNTLIFLLPILVVLFLNATSSDSGSGDDRT
                     RILWQSYDVHIVVSEDGNLHVTERQNVRFYGAFSEGYARIPLDRVGSIDNVQVMTENA
                     PEDRDNNGFL"
     gene            complement(513..2492)
                     /locus_tag="VGR29_08610"
     CDS             complement(513..2492)
                     /locus_tag="VGR29_08610"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV2073686.1"
                     /translation="MTMTLPGSRHRCWLRAFATLALITLFFGHMGVPLTTAQSGNRVV
                     WDEYNVDFVVNDDGTVRVIETQVVEFDGRFSTGFANIPLTGTEAITNVTVSIADSASE
                     EPQIAEQVRFFNEEPGTYAVEENAGTLEIDYAYEPTSPLGSAADNTRVIVIEYELIGA
                     IRVYEDLEPANQQLRWVAIETDVTEIAPIRSSTVTVTLPEPVDTAQTVARPKGVETDG
                     QTFTWRQSNLDNGDRFEVNLQFPPITAAAKPAWQDRFDQIREQQITAAGQSAAAGTLF
                     LGAGLLLLVGGSLLLAGLWYTRGRDPDVRLVADIVAEPPDDLRPGAAGTLIDEETHTR
                     DVIATMLDLARRGIIHLDEKQTEGFMGFGRQTRYILTLQEHSNELMGYEQVLLDAIFG
                     PNAEVGSSVPMEQVQQTFAARADTIHNGFYDELVEHGYFDASPEQTRQHARMLGCAGP
                     ILAAVVIFLVIFFTGASSGFIVLPILAAVVLFAIGRKVATNMPRKSLKGAEAAAKWRA
                     FKRYLQEIEQHENLEESKAIFDRYLPYATAFGLEDSWIRKFSQVSTPMPGWFGGGFGG
                     PVVLGQPGGYGRRSSPFGGGMWVFPSGGGSFGGGSVGGGSRGGDASGGGFDMPGMQDL
                     SDSAGGSLQSGSDSIFDMLGTASEIFGGGGGRGFG"
     gene            2694..3203
                     /locus_tag="VGR29_08615"
     CDS             2694..3203
                     /locus_tag="VGR29_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEV2073687.1"
                     /translation="METHEHHIHQTESLALSRPEVERVLGEHGYRLTAPRSAIVDAVL
                     GHSRPFSAEQLVTELSETPGGPRIGRATVYRTLEVLAAIDVLTRIVQPDGHPSYIWGT
                     PGHRHHLLCESCGTTVTFTACPLDDLVKDLTRNTDFEIRNHVLEVFGMCPACRRGDRT
                     TSPTLTSAY"
     gene            3343..4140
                     /locus_tag="VGR29_08620"
     CDS             3343..4140
                     /locus_tag="VGR29_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV2073688.1"
                     /translation="MVIPGPHQPDREALLTARELSVHYEERSALRDITLDIYPGRTVS
                     LLGPNGAGKSTLLKVFAGMIPPSHGSVEFAGTPLSGAHESVTYVPQRTGADWTFPISV
                     LEATLLGLSRSRPRWRPFSRRDRDQAMQALQEVKMDHLSSAQIGALSGGQQQRVFLAR
                     ALLACGQVLLLDEPFTGVDIPTQEMLVELFERLSNEGTAVVYATHDLQHAASTSDEVV
                     LLNGHIVAQGSPANVMSEKILQTTFGGQVIVIHTGNTSAPIPDEAHI"
     gene            4140..5036
                     /locus_tag="VGR29_08625"
     CDS             4140..5036
                     /locus_tag="VGR29_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV2073689.1"
                     /translation="MMAWLTDPWQHTFMQHAFIAITLVGIICGAMGVFVTLRGLAFLG
                     DALAHAIFPGVVIAFILGGNFLIGAIIAAIIVSLGIGAISQSGRINNDTAVGVLFVGA
                     FSLGIALMSTQSGYVRDLNAFLFGSILGVTRSDLALTIAVGMIVLAALLLFRRELTMI
                     SFDRTFSKSNGLNLWLYDQLFLVLLAMAIVISLQTVGNILVLAMLVTPAATARLLTND
                     LRTMVVLSCLTGSFAGVTGLYVSYHQGVPSGAAVVLVSTTIFAIVFLFEPRTGAISNR
                     IRRRLHHPHPEKDDFPASRPAA"
     gene            complement(5090..6031)
                     /locus_tag="VGR29_08630"
     CDS             complement(5090..6031)
                     /locus_tag="VGR29_08630"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2073690.1"
                     /translation="MKRERRQQQGPWGTMAEALLVTTLAFAVLWQIQGQVGSSPSSGS
                     VPEETARTSETRNFVTTADVNLRTGPGISHSVLSVLPPQSQIDVIGVERDGFALVQSG
                     GQEAWISVEFIAPETEVLAATVNGPEGIEPTAAPAQIPTEAPTEAVVPDAAVVGGDIA
                     AASTDAETYKATQQEPEAEVANVPAGERWVEVDRSTATVTLHEGYRTVAVFQGKIGKD
                     PATDGYYSTAVGTFHVFSMNQELTQTPFAPGVYLTDWVGFDPDRSNGFHSPVRDADGN
                     VVETGGTTTLGCVRLGEDEARQMFDFAFIGMRVEIHD"
     gene            complement(6110..6880)
                     /locus_tag="VGR29_08635"
     CDS             complement(6110..6880)
                     /locus_tag="VGR29_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV2073691.1"
                     /translation="MGPFAQAGAVGQIGSLSTGGRLVIRVVMALMLAMTLLGALAGLS
                     AKDASAGQAGTINTDGVGLMAGFTDQSIIEWMYAGERVDVLWGPEDGKYEVRYYGVDG
                     WVWAEYLTVDGVGGGSVTYDAPAEVAYEEHWIDINRTQGSVTLYIGDTPQATYWASMG
                     WDTSDYGYYSTAVGTYYVNWMDSSLQYTPFADAYITHWIGFDWERSNGFHSYTKDANG
                     DIVPNGAGKTGGCVALAPGDIDAVYNFAFPGMRVEIHN"
     gene            7688..8161
                     /gene="greA"
                     /locus_tag="VGR29_08640"
     CDS             7688..8161
                     /gene="greA"
                     /locus_tag="VGR29_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV2073692.1"
                     /translation="MASTRGVQLTRQGKERLEEELRQLLKEKLPQLTQAMQYANEEGD
                     TNEHSEYEDLKEERVHIDNRIREIEYTLEHAILIEEGSKDGTIALGSHLSVDTDDGEP
                     LEHWVLVSAEEADPLHGRISDDSPVGQQLLGKKVGDTFKVETPGGEVTYTVVEVK"
     gene            8270..9799
                     /gene="lysS"
                     /locus_tag="VGR29_08645"
     CDS             8270..9799
                     /gene="lysS"
                     /locus_tag="VGR29_08645"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV2073693.1"
                     /translation="MELSDLQEIRRAKAEALCDRGVSPYPTRAERTHTTKAALELFTS
                     EEEAGTLHDGHTGETVILTGRVVSFRHMGKTVFAHIRDGFGELQLYIRKDVVGEEAYA
                     DILKLFDLGDFVQATGDLFRTKMGEISLRARSVTMLTKALNAPPEKWHGLQDVELRYR
                     QRYADLLANAETRKVFATRSRIVSAIRHFLDERGYLEVETPVLQPLYGGAAARPFTTH
                     HNTLDQTFYLRIADELYLKRLLVGGFERVYEISKDFRNEGIDRNHSPEFTMLEFYQAY
                     GDYEQMMEMVEQLLSAVVKEVYGGSTFVYQGNEIDVTPPWPRRTLREAIMERTGIDYT
                     EHADADDLLAAARKAGADIETGTVWPRIVDELLKQFVRPHLLQPIFLIDYPLELSPLA
                     KRKPDDPTHVERFQPYIGGGEVGNAFTELNDPMDQLQRFIEQQKDREAGDEEAMPVDE
                     DFVNALMYGMPPTGGVGIGIDRLVMLLTDQATLRDVILFPAMRRLPASSTDAGAGDDD
                     Q"
     gene            9905..10048
                     /locus_tag="VGR29_08650"
     CDS             9905..10048
                     /locus_tag="VGR29_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073694.1"
                     /translation="MGIKDTIQDKTGIYKLGFDPASFAGILLPLLFVVIVGGALAALV
                     LLR"
     gene            10346..12736
                     /locus_tag="VGR29_08655"
     CDS             10346..12736
                     /locus_tag="VGR29_08655"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2073695.1"
                     /translation="MSVPVPPDRLGRDPVAVLPFQGRPPPGIGTVPLPLTPLIGREGE
                     LAAVVALLHHQDVRLVTLLGPGGVGKTCLALWATVHLGPAFPDSIVFIDLAPVSAPAL
                     VAPAIAQALNVIQSGNAPLVERLASHIGDRRLLLVLDNFEQVVEAAPTIAALLGACPA
                     LTVLATSRVPLRLTGEHEHVVPPLALPGEHDASSIERIAASEAVRLFVARAQDVREAF
                     ELTAESAEAVAAICRRLDGLPLAIELAAARVKVLPPAALLSRLERRLPLLTGGQRNRP
                     DRQRTMRDTIAWSHDLLSPAEQGLFQRLAVFVGGFSLEAAEAVAGRSVEVLEGVASLL
                     DSSLLDRVAGVGEDPRFTMLETIREFALEHLTTSGEEGAVRDRHATYFAELGEQTEIH
                     LLREVESVWLDRLEADHDNLRSALHWTLVAEANPASAELGLRLAGALWLFWYYHSHLT
                     EGRWWLEQGLSAAGSVPDAVRAKALVGLGTLAHAQGDESRSLAWLSEGLLLLRAIGDT
                     WGLAFALTVRGNLAEDAGQFDEAEIYFTEANALFADADDGVNVAVTLYHLGVVAFGQN
                     QLELAESRCTRALQLSRALHDPWGTAISLAYLGLVQNLRGDVEQAATSLGEALTLFVQ
                     IGGVERIADMLSRIAVLAEARGKHVVAVRLFAAAEEQREHIGAVQTLPERAVYEQALT
                     TLQGQIPADDFAAAWVAGKALPLDQAVAEARAAIGNQATESVASKDMPVSFAALTPRE
                     VEVLHLLTQGRSNREIAQELFISPRTAAGHVTSILEKLDLPSRAAAAAFAVRHGLT"
     gene            12966..>13375
                     /locus_tag="VGR29_08660"
     CDS             12966..>13375
                     /locus_tag="VGR29_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2073696.1"
                     /translation="MVQTELSRRRLLKQGGSAIAGLSVLRLSGPTYAFQTPVTGDVIP
                     WLDQPEPNPAPEAIVRQLEWEQLDSWITPVDQFFVIKHYNEPALTEADYRLEVSGLVE
                     NPLTLTLADLKGRERQEVTFTMECSGNSAFPFNKG"
BASE COUNT         2603 a         4255 c         3868 g         2649 t
ORIGIN      
        1 tgagaaagcc gttattgtct ctgtcctccg gagcgttttc ggtcatcacc tgcacgttgt
       61 cgatcgagcc cacccggtcc agcggaattc gggcatatcc ctcgctgaaa gcaccataga
      121 accgcacgtt ctgccgctcg gtgacatgga ggttgccatc ctcgctgacg acgatgtgga
      181 catcgtagga ctgccagaga attcgcgtgc ggtcatcacc gctcccggaa tcggaggaag
      241 tagcgttcag gaacaagacg accaggattg gcagcaggaa tatcagggtg ttcagacacc
      301 ccttggcaat tttttcgtct cgtgttccct gatccatcac cgtctcctgc cgcgcctaca
      361 acctgggtgt catgcccgtc cactgcatag acaactgact caggagttct tcggttccct
      421 ttcaacgtcc cgtaagccca aaaacatcct ggccagcgct ccgtaaagcg ccaaccagga
      481 tgtcgttagc catttgcact atggtgaagc gattatccga aaccccggcc cccgcctccg
      541 ccgaagatct ccgacgcggt gcccagcata tcgaagatac tatcggaccc gctctgcagg
      601 gaaccacccg ccgagtcact caggtcctgc atgccgggca tgtcgaaccc gccaccggat
      661 gcgtccccac cgcgactccc accgccaacc gagccacctc cgaaggatcc gcctccggac
      721 ggaaacaccc acattccacc gccgaatgga gaactgcgtc gaccgtaccc accaggctgc
      781 ccaaggacca ccggaccacc gaagccacct ccgaaccaac ccggcatcgg tgtgctgact
      841 tgcgagaact tgcggatcca ggaatcctca agaccgaatg ccgtcgcata ggggaggtag
      901 cgatcaaaga tcgccttcga ctcctcgagg ttctcgtgct gttcaatctc ctgcaagtag
      961 cgcttgaagg cgcgccattt cgcagcggcc tcggcgccct tgagggattt ccggggcatg
     1021 ttggtcgcaa cctttcgacc gatagcaaag aggaccaccg cagccagaat tggcagtacg
     1081 atgaagccgc tgctggcccc ggtgaagaag atgacgagga agataacaac agccgccagg
     1141 atcgggccgg cgcatcccag catccgcgcg tgctggcgag tctgctcagg tgacgcgtcg
     1201 aaatacccgt gttccaccag ctcatcatag aagccgttgt ggatagtatc ggcacgggcc
     1261 gcgaaggttt gctgcacctg ttccatagga acgctgctgc ctacctcagc atttggaccg
     1321 aagatggcgt cgagcagaac ctgctcatac cccatcagct cgttggagtg ctcctggagg
     1381 gtgaggatgt atcgcgtttg ccggccgaac cccatgaagc cttcagtttg cttttcatcg
     1441 aggtgaataa tgccgcgtct cgcgagatcc aacatcgtgg cgatcacatc acgggtatgc
     1501 gtctcctcat cgatcagcgt acccgccgca cccgggcgga gatcatcagg cggctcggcg
     1561 acaatgtcgg ccaccagtct gacgtccgga tctcgaccgc gggtgtacca gagtcctgcg
     1621 agcagtaagg atccgccaac aagcagcaac agtccagcac caagaaagag cgtaccagcc
     1681 gcagccgact ggcccgcggc agtgatctgc tgctctcgaa tctggtcaaa gcggtcctgc
     1741 catgccggct tggcagcggc ggttatcggc ggaaactgga gattgacctc gaagcgatca
     1801 ccgttatcga ggttactctg cctccaggta aacgtctggc catcagtttc cacgccttta
     1861 ggccgtgcta ctgtctgtgc agtgtcgacc ggctccggga gggtgacggt aaccgtcgag
     1921 ctccggatcg gagcaatctc ggtgacatcc gtctcaatcg ccacccagcg taactgctgg
     1981 tttgccggct cgaggtcctc gtacacgcga atggcaccga taagttcgta ttcgatgacg
     2041 attacgcgcg tgttgtccgc ggcggaccca agaggactcg tcggctcata ggcgtaatcg
     2101 atctccaggg tgccggcgtt ttcctcaact gcatacgtgc cgggttcctc gttgaagaac
     2161 cgcacctgtt ccgcaatttg cggctcctca ctggcactgt cggcgatgct gacggtgaca
     2221 ttggtgatag cttcggttcc ggtcaatggg atgtttgcaa aacctgtact gaaacggcca
     2281 tcaaactcga ccacctgggt ctcgatgaca cgcactgtgc cgtcgtcatt gacaacaaag
     2341 tcgacgttgt actcatccca gacaacgcga ttaccagact gcgcggtcgt gaggggcact
     2401 cccatatgcc cgaagaaaag cgtaatgagc gccagcgtgg cgaacgcacg gagccaacac
     2461 cggtgtcgac tcccggggag cgtcatcgtc atcgtggtca agtcctgtcc tcgtcaaaga
     2521 ttcaacggat cttatgcttc aagtatcgca tttcgccgga gcggcatcgg tgccatatcc
     2581 tactccacga atggtgcaat tgatcccatt cgcacgatca tcgccatgct ggcgcttgaa
     2641 gtccttgttc ccagatgaga cagagtccca tctggacgag tatcatagat ggcatggaaa
     2701 cacacgagca ccatatccac cagaccgagt ccctggcact ctcccgacca gaggtcgagc
     2761 gtgtgctggg cgagcatggt taccgcctga cggcaccgcg gtccgcgatt gtcgatgcgg
     2821 ttcttggcca ctcccggccc ttttccgccg agcagcttgt cactgaactg agcgagacgc
     2881 cgggtggacc ccgtatcggg cgcgcgacag tctaccggac cttggaggtc cttgccgcta
     2941 tcgacgtgct gacccgtatc gttcagccag acggtcatcc gagctacatc tggggcacgc
     3001 ccgggcatcg gcatcacctt ctctgcgaga gctgtggtac caccgtgacg tttactgcat
     3061 gcccgctcga cgatctggtg aaggacctca cgcggaacac cgacttcgag atacgaaatc
     3121 atgtgctcga agtctttggc atgtgcccgg cgtgcagacg cggcgatcgg actaccagcc
     3181 caacgcttac atccgcttat tgacacttcg tatcaataga ggtattctcg ggaacaacga
     3241 cacctcgcct cgcgacgtac cccggggaca tccctgttca tggcgcaccc tgtttccggt
     3301 ccgtagcggc acacaatact tccaggtcag gtagatctcc tcatggtcat tccaggaccc
     3361 catcaaccag accgcgaagc actcctgaca gcaagggaac tctcggttca ctacgaggag
     3421 aggtcggcgc tccgtgatat caccctggat atctatcccg ggcgcacggt cagcctgctg
     3481 ggaccaaatg gtgccggaaa gagcacgttg ctcaaggtct tcgctggaat gatcccgccc
     3541 tctcacggtt ccgtcgagtt tgcaggtact ccattgagcg gcgcccatga gagcgtcacc
     3601 tacgttcctc agcgcacggg agcagactgg accttcccca tctccgtcct tgaggcgacg
     3661 ctccttggcc tctctcgctc ccgcccccgc tggcgcccct tcagccggcg tgatcgcgac
     3721 caggcgatgc aggcattgca ggaagtgaaa atggaccacc tgtcgtccgc gcagatcggc
     3781 gcactctcag gcgggcagca gcaacgcgtt tttcttgcgc gggcactcct tgcctgcggc
     3841 caagtcttgc tgctcgacga gcccttcacc ggtgtggaca tccccacgca ggagatgctc
     3901 gtcgagcttt ttgagcgcct cagcaacgaa ggcacagccg tcgtttacgc gacgcatgat
     3961 ctccagcatg cggcgagcac ctccgatgag gtggtcctgc ttaacgggca tattgtcgcg
     4021 cagggttcac ccgccaacgt gatgagcgag aagatcctcc agacgacgtt cggtgggcag
     4081 gtgatcgtga tccataccgg taacaccagc gccccgatcc ctgacgaggc acatatctga
     4141 tgatggcgtg gctcacggat ccctggcagc acaccttcat gcagcacgcc ttcatcgcca
     4201 ttacgctagt gggcatcatt tgcggcgcga tgggtgtctt cgttacctta cgtggtctcg
     4261 cctttctcgg tgacgctctc gcccatgcaa tctttccggg ggtcgtgatt gccttcatcc
     4321 tgggcggcaa tttcctgatc ggtgccatta ttgccgccat catcgtctcg ctcggaattg
     4381 gagcgatcag ccagagcggg cgcatcaaca acgacacggc cgtaggtgtg ctcttcgttg
     4441 gcgccttttc actcgggatc gcactgatgt cgacccaaag cggttatgtc cgggatctca
     4501 acgccttcct ctttggctcg atcctcggtg tcacccggag cgatctcgcc ctcacgattg
     4561 ccgtcggcat gatcgtgctc gccgcgttgt tgctcttccg gcgggagctg acgatgatct
     4621 cgtttgaccg caccttcagc aaatccaatg gcctcaacct ctggctctac gatcaactct
     4681 tcctcgtcct gctcgctatg gccatcgtta tttccttgca gacggtgggg aatatcctcg
     4741 tgctggcgat gcttgtgacg cccgccgcca ctgcccgcct gctgaccaat gacctgcgca
     4801 cgatggtcgt cctctcttgc ctgaccggct cctttgccgg tgtcacgggc ctttatgtct
     4861 cctatcatca gggcgtccca tcgggcgcgg ccgtcgtgct cgtctccacg acgatcttcg
     4921 cgatcgtctt cctgttcgag cctcgaaccg gcgcgatcag caatcggatc cgacgccgac
     4981 tccatcatcc ccatcctgaa aaagacgatt tcccggcatc ccgaccggcg gcgtagtgtc
     5041 gcaaggaagc agaccctgcc ggctgccggc taccaacggg gcatctctgc tagtcatgga
     5101 tctcgacgcg catgccgatg aaggcgaaat cgaacatctg ccgggcttca tcctcgccga
     5161 gccggacgca cccgagcgtg gtcgtgccgc cagtctcgac gacgtttccg tccgcgtcgc
     5221 gcaccggtga gtggaagcca ttgctccgat ccggatcgaa ccctacccag tcggtcagat
     5281 agacaccggg tgcgaaaggc gtttgggtca gttcctggtt catggaaaag acgtggaacg
     5341 tcccgacagc ggtggagtaa tagccatccg tggcggggtc cttgccgatc ttgccctgga
     5401 atacagccac ggtcctgtag ccttcatgca gggttaccgt cgccgttgac cgatcaacct
     5461 caacccaacg ttccccggca gggacattag ccacctccgc ttctggttcc tgctgggttg
     5521 ccttataggt ttctgcatct gttgatgcag cagcaatgtc cccgcctaca acggccgcat
     5581 ccggaacaac agcttccgtg ggtgcctcag tcggaatctg ggccggggca gcagtcggct
     5641 cgatgccctc cggcccgttg accgttgcgg ccaggacttc agtctcgggt gcgatgaact
     5701 ccacggagat ccacgcttct tgtcccccgg attgcaccag agcgaaaccg tcccgttcca
     5761 ccccgatgac atcgatctga ctctgcggcg gcaatacgga aagcacgctg tgtgatatgc
     5821 cgggccctgt ccgcaggttt acgtccgctg tcgtcacgaa gttgcgcgtc tctgatgttc
     5881 gggcagtttc ctccggtacg ctcccgcttg agggactgct ccccacctgc ccctggattt
     5941 gccacagcac ggcaaaggcg agagtggtga caagcagagc ctctgccatg gttccccagg
     6001 ggccctgttg ctgccttctc tcgcgcttca ccgtacgttc ctcgtcttgg ccttgggagc
     6061 gggaacccct cctgatgtct tccatccgaa aggagtcccc acgccgctgc tagttatgga
     6121 tctcgacccg catgccgggg aaggcaaagt tgtacaccgc gtcaatatcg cctggcgcca
     6181 atgcaacgca accaccagtc ttgcccgcac cgttgggcac gatatctccg ttcgcgtcct
     6241 tggtgtagga gtgaaagccg ttggatcgtt cccagtcaaa cccgatccag tgcgtgatgt
     6301 atgcatccgc aaagggcgtg tactgaagtg aactgtccat ccagttgacg tagtaggtgc
     6361 cgacagccgt ggagtagtat ccataatcgg atgtgtccca ccccatcgac gcccaatacg
     6421 tcgcctgagg cgtgtcgccg atatagagtg tgaccgatcc ctgggtgcga ttgatatcga
     6481 tccagtgctc ttcataggcg acctcggcgg gcgcgtcgta ggtgaccgac ccgccaccaa
     6541 cgccatcgac tgtcaggtac tcggcccaga cccatccatc tacaccgtag tagcgaacct
     6601 catactttcc gtcttccgga ccccagagga cgtcaacccg ctcacccgcg tacatccact
     6661 cgatgatcga ttgatccgtg aagccagcca tcaagccaac gccatcggta ttgattgtcc
     6721 ccgcctgtcc ggccgacgca tcctttgctg ataatccggc caaggccccg agcaacgtca
     6781 tggcgagcat caacgccatg accacgcgga tgaccaaacg tcctcccgtg gatagactgc
     6841 ctatctgtcc aactgcacca gcctgcgcaa acggtcccat agctactccc cctccggagc
     6901 taattgcggg taggttcgaa tcagaacgat cgcctgcggg aacgacggtc cagccctgtc
     6961 cctttgccag ccgcaaagag tgtacgtacg acctgtacgt atagtagcga ttacttcccc
     7021 gcggtgcaac cccttatttg tgcttccttg agcggcatca ttgctcgtca aagtagccgc
     7081 ctgacgagga accccttatt gtgactgatt gtcacaataa ggggtcggac gagaatgagg
     7141 ctcgatgaga tttgataaac acgaatactg ggaggtcaag tgttgggcct tgtacaaaga
     7201 atgcctgagc atagggatct aaactatatt gtgggaatcg ttggagcggc ccatcccttt
     7261 actgcttgtg ccaacaggca cacctggcaa taccttgatc cacttcgacg ggctcgacac
     7321 tatggggaac ggccagaacg taggttcccc attgcgtttg ctggcaaagc gcctatactt
     7381 gaacgcaact agggaaggcc gggggcatga tcctccggct ttgtcctata cgccaaatac
     7441 cagcgtgagt gtgggttcgt cgccatcgat acaaatcagg ggcgacctcg aacgatcagt
     7501 tcggggtcta tgtacacatc ctggccagtc gtcttcggag ttggttgggg tccccactat
     7561 cgttcgaacg atcccgctct cgacggcatt cggaatgcga tgttcaccgc gaacgaccag
     7621 tcaaccacca tatgtagtcg cctgtccgtg cattggtaag gggcgggtgt aaggaaagga
     7681 ccttctcatg gcatcaacac ggggagtgca gctgacccgg cagggcaagg agcgtctcga
     7741 agaggagctc cgacaactcc tgaaggaaaa gctgccccag cttacccagg cgatgcaata
     7801 cgccaatgag gagggcgata cgaacgagca cagtgagtac gaggatctca aggaggagcg
     7861 cgtccacatt gataaccgca tccgcgagat cgagtacacg cttgagcacg cgatcctgat
     7921 cgaggaaggc tccaaggacg gcaccattgc ccttgggtct cacctttcgg tcgacaccga
     7981 cgacggcgag ccgctcgagc actgggtcct ggtcagtgct gaagaggccg atccactcca
     8041 cggccgcatc tccgacgact ccccggtcgg ccagcaactc ctcggcaaga aggtcggcga
     8101 cacattcaag gttgaaacgc ccggcggcga ggtgacctac acggtggtgg aagtaaaata
     8161 accatcgagt gcacacttcc ggcaggcaac gcggcgggca cttcccgccg cgttttccat
     8221 gcctgccagc aaacatcgat catgccaagc ccttgtaacg gagcacctga tggagctaag
     8281 tgacctgcag gagatacgcc gtgcgaaggc cgaagccctt tgcgatcgtg gtgtctcccc
     8341 ctacccgacc cgcgccgaac gcacccacac gacaaaggcg gccctcgagc tcttcacatc
     8401 cgaagaggaa gctgggacgc tccacgacgg acacaccggt gagaccgtca tcctcacggg
     8461 ccgtgtcgtg agctttcgtc atatgggcaa gacggtcttc gcccatatcc gcgacggttt
     8521 cggcgagctc cagctttata tccgcaagga tgtggtcggg gaggaggcgt atgccgacat
     8581 cctcaagctc tttgacctgg gcgatttcgt gcaggcgact ggcgacctct ttcgaacgaa
     8641 gatgggggag atttccctgc gagcccgctc agtcaccatg ctaacgaaag cgctgaacgc
     8701 tccgccggag aagtggcacg ggcttcagga tgtcgagctg cgctatcggc aacgctacgc
     8761 ggatcttctc gcgaatgcgg agacacgcaa ggtcttcgcc acacgctcca ggattgtctc
     8821 cgcaatccgg catttcctcg acgagcgcgg ctatcttgag gtggaaacac cggtgctgca
     8881 accgctctat ggtggagccg cggcacgtcc ctttacaacg catcacaata cgcttgatca
     8941 gaccttctac ctccggatcg cggatgagct ctatctcaag cggctcctcg tcggcggctt
     9001 cgagcgcgtc tacgaaatat ccaaggactt ccggaacgag ggtatcgatc gcaatcactc
     9061 gcccgaattc acaatgctcg agttctacca ggcctacggt gactacgagc agatgatgga
     9121 gatggtggag caactcctct cagcggtggt gaaggaggtc tatggcgggt cgaccttcgt
     9181 gtatcaagga aacgagatcg atgtcactcc gccctggcca cgcaggaccc tgcgggaggc
     9241 gatcatggag cggacaggga tcgactacac ggaacacgcc gatgcagacg atctcctcgc
     9301 cgcggcgcgg aaagccggcg cggacatcga gacggggacc gtctggccaa ggattgtcga
     9361 cgaattgctc aagcagtttg tccgtcccca tcttctccag ccaatcttcc tgatcgatta
     9421 cccgctagaa ctctcaccgc ttgccaagcg caagccggac gatccgaccc acgttgagcg
     9481 gttccagcca tacatcggtg gcggtgaagt cggcaacgcc tttaccgagc tgaacgaccc
     9541 gatggatcaa ctccaacgct tcatagagca gcaaaaggac cgcgaggcgg gcgatgagga
     9601 ggccatgccg gtcgacgagg acttcgtcaa tgcactgatg tatggcatgc ccccgacagg
     9661 aggcgtcggt attggcatcg accggctggt catgctcctc acggaccagg caacacttcg
     9721 cgacgtcatc cttttccctg cgatgcgccg tttgcccgca tcgtccacgg atgcaggcgc
     9781 cggggacgac gatcagtagc atgtctgcca ggctccatat atgctgatat cgtcaggatg
     9841 tgctctattg ggagaacgca acgatgctgc ggcacttccc cggaggcaag cagaggagtc
     9901 gaacatgggt atcaaggaca ccattcagga caagaccgga atctacaagc tgggcttcga
     9961 ccctgcgtcg tttgcgggta tcctcctgcc gctgctattc gtcgtcatcg tgggtggcgc
    10021 tctcgccgct ctggtgcttt tgcgataaac aacctcctcc atacagaggc cgggaatcgt
    10081 gctgatctcc caatcagcac ggttttcttt gttactcgct tcgtcttgct acgggggtcg
    10141 ctcccgcatc ctcggtgtag ggcatagctg aatcggggcg gacgggaccg gccgccgata
    10201 cccatgcccc accggcggcg gctcccgctc gcaataaccc ggcgcattgc acccctgctg
    10261 cgccgggggc tgcagttgac atctcggcac ctgacaggga tgatggcagt gtcgacccaa
    10321 ctcaccctca gcacgaggcg gtcacatgtc tgtgccggta ccacccgacc gcttgggacg
    10381 cgatcccgta gcggtccttc ccttccaggg ccgtccccca ccagggatag ggaccgttcc
    10441 cctcccgctg accccgctca tcggccggga gggagagctt gcggccgtcg tcgccttgct
    10501 tcaccatcag gacgtccgcc tggtgaccct cctcggtccc ggcggggtgg gcaagacctg
    10561 ccttgccctc tgggcgacgg tgcatctcgg tcctgccttt cccgacagca tcgtcttcat
    10621 cgacctggcg ccggtctccg ccccggcgct ggtcgccccc gcgattgcac aggccctcaa
    10681 cgtcatccag agcggcaatg caccactcgt cgagcgcctg gcctcgcaca tcggtgaccg
    10741 gcgcctgctg ctcgtactgg acaacttcga gcaggtggtc gaggcagcac cgaccattgc
    10801 cgccctgctg ggcgcctgcc cggccctgac cgtcctggca accagccggg tgccccttcg
    10861 cctcaccggt gagcacgagc atgtcgtccc gccactcgcc ctgcccggcg agcacgacgc
    10921 gtcctccatc gagcgcatcg ctgcgtccga ggcggtgcgc ctctttgtgg cacgggcgca
    10981 ggacgtgcgg gaggcattcg agctcacggc cgagagtgca gaagccgtgg ccgccatctg
    11041 ccgtcgcctg gacggcttgc cgctggcgat cgagctcgcg gcggcccggg tcaaggtgct
    11101 tccgcccgcg gcgctgctct cccgcctgga acgccggcta cccctgctga cgggggggca
    11161 gcgcaaccgg ccggaccggc agcgcaccat gcgcgatacg atcgcctgga gccacgatct
    11221 cttgtccccg gcggaacaag ggctcttcca gcgcctggcc gtcttcgtcg gcgggttctc
    11281 gctcgaggcg gcggaggcgg tggcgggcag gagcgtggag gtgctggagg gggtggcatc
    11341 gcttctggac agcagcctgc tggaccgggt ggcgggcgtg ggggaagacc cgcgcttcac
    11401 gatgctggaa acgattcgcg agttcgccct cgagcacctg accacgagcg gcgaggaagg
    11461 ggcagtccgc gaccggcatg ccacctattt tgcggagctg ggcgagcaaa cggagatcca
    11521 cctgctccgg gaggtcgaat ctgtttggct ggatcggctg gaagccgatc atgacaatct
    11581 acgaagcgca ttgcactgga cacttgtggc ggaagccaat ccagcttcgg cggagcttgg
    11641 gctgcgactc gccggcgcgc tgtggctctt ctggtactac catagccatc tgaccgaagg
    11701 tcggtggtgg ctcgagcagg ggctgtcggc tgcagggtcg gtgccagatg ccgtgcgagc
    11761 gaaggcactt gtgggcctgg ggacgctcgc tcacgcccag ggcgacgagt cccgctcgct
    11821 cgcgtggctt tcggaaggtc tcttgctcct gcgtgcgatc ggggatacat gggggttggc
    11881 cttcgcactc accgtgcgcg gtaacctggc tgaggacgcg ggccagttcg acgaggctga
    11941 aatctatttc accgaggcga acgcactgtt tgccgatgca gatgatggag tcaatgtcgc
    12001 tgtcacgctc taccacctcg gagtcgtcgc cttcgggcag aaccaactgg aactggccga
    12061 gagtcggtgc acccgcgcgc tccaacttag ccgggcactg cacgatccat ggggcactgc
    12121 tatctcgctc gcctacctcg ggcttgtaca gaacctccgt ggcgacgtag agcaggcggc
    12181 gacatcgctg ggcgaagcgc tcacactctt tgtccagatt ggaggggttg agcgtatcgc
    12241 cgatatgctg agccgcatcg cggtactggc cgaggcgcgc gggaagcatg tagtggccgt
    12301 ccgccttttc gccgccgctg aagaacagag ggagcacatc ggagccgtgc aaacgttacc
    12361 cgagcgtgcc gtctatgagc aggcgttgac tacgttgcag gggcagattc ccgcggacga
    12421 cttcgctgcc gcctgggttg cagggaaggc tttgccattg gaccaagccg tcgccgaagc
    12481 tagggcagcg atcgggaacc aggcgacaga gagcgttgcc agcaaggaca tgccagtgtc
    12541 gttcgcagcc cttacgccta gggaagttga ggtgttacac ctcctgaccc aaggacgctc
    12601 caaccgcgag attgcccagg aactcttcat cagcccccgc actgccgccg gtcacgtcac
    12661 cagcatcctc gaaaaactgg atcttccgtc ccgtgcggcc gctgctgcct tcgccgtccg
    12721 ccacgggctc acctagccca gccacctttt gcaggaacta cctacacccg aagacgcatc
    12781 tccatcacca aatacgtagt tcttacgatg cgatgcggac cgctgtgaaa caccatggaa
    12841 cgtgtcacgg tgcctaaaac cggttccacc atcgtgttgg tgtgaccccg cgaccacgca
    12901 ggtgccgtca gggggctcgt atcagatggc ttcggtcacc tgccacacga gcaaggagac
    12961 gaatcatggt gcaaacggaa ctatcccgca gacgactgtt gaagcagggc gggtcggcga
    13021 ttgccggcct cagcgtgcta cgactgtcgg gaccaacata cgcgttccag accccggtga
    13081 cgggggacgt catcccctgg ctggaccaac cggagcccaa ccccgcgccg gaggcgatcg
    13141 ttcgccagct ggaatgggag cagctcgatt cctggattac tccagtggac cagttcttcg
    13201 ttatcaagca ctataacgaa cctgcactga cggaagccga ctatcgcctg gaagtcagtg
    13261 gcctggtcga gaacccactg acgctgaccc tggctgacct gaagggacgt gagcgccagg
    13321 aagtgacctt cacgatggag tgttccggca actcggcgtt ccccttcaat aaagg
//
LOCUS       DASYWM010000213        11386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_139974, whole genome shotgun sequence.
ACCESSION   DASYWM010000213 DASYWM010000000
VERSION     DASYWM010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11386
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139974"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VGR29_08665"
     CDS             <1..705
                     /locus_tag="VGR29_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073697.1"
                     /translation="SIASQTWHTTDMGNQQEMLELALPSGEHVEVTFAEASPDDSLAG
                     EGQDRTGKMEELMQRALEFSEGNPPHHPGTLPRFPVPSASYRDTIAVPMTVLAVDDAG
                     ARGLYAPPRQVGISIKDGSLVGAGEFPGFDPEHWPPARLGDWPPPQLAGIPHVQLQAM
                     IARFSACWSRVLDAWFAKVAEADDILRQDVREGLHRRSILDLMEMLQYYDRLNPVFAK
                     WLNDMVGEDQHDEETR"
     gene            complement(747..1697)
                     /locus_tag="VGR29_08670"
     CDS             complement(747..1697)
                     /locus_tag="VGR29_08670"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2073698.1"
                     /translation="MTPELWIRLAIGVGLVVVSVYTNRSFLIWGLIALLAVIFVPTGR
                     ARSFVASIVPYATVWFVFTFLRSFADETILAKTLNTKAVEFERWLFGGELPTIRLQAA
                     YYDPGSIHWYDYYFTFIHWSYFIVPHAVVVWLWWKNQRRFQHYLSALTMLLMLGLLIY
                     FIIPSNPPWMAPESIDSPGAPVVLRIMEPIARELGGGLYQAGYKIVGESNPIAAMPSI
                     HFAVTFLLVFAAKGRGRGWELGSWLYALSMGAALVYMGEHYVIDIAVGGVVTAYSWAV
                     TGAWLRRQDHIISPTHFGPPHEASEPVTPHREDATQPARV"
     gene            complement(1901..2422)
                     /locus_tag="VGR29_08675"
     CDS             complement(1901..2422)
                     /locus_tag="VGR29_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEV2073699.1"
                     /translation="MYWTERHVLVCTGSHCIQKGANQVLTRFRFELMRKRLNTRIHLN
                     TCGTIDLCDIGPNFVIYPDNIVYSNVSENDIPDIIRFLEGGPVVERLVTGPTAPAEQN
                     RQRFYAALKERDNAWTENDVNALAADHSLDERWIAEQLRRGFMAKKPDPDSAENRFSM
                     TSKALHRYRLESR"
     gene            complement(2820..3936)
                     /gene="prfB"
                     /locus_tag="VGR29_08680"
     CDS             complement(join(2820..3860,3862..3936))
                     /gene="prfB"
                     /locus_tag="VGR29_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922581.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV2073700.1"
                     /translation="MASRDDLVKSLSELATRLDSLRGRLDLANDEREIEDLERRSSDP
                     EYWNDPTTAQNEMRRLGEIKARIDNWTQAEQSVADMLDLLEMAEDEPELIEEISAERD
                     SLLQKVDSMELQSALNEPHDRSDAILIIHSGEGGVDAQDWTFMLSRMYLRWADKRGFK
                     ADLLDTTEGDEAGIKNATIEVRGSNAYGLLKGEGGSHRLVRLSPFDSAHRRHTAFALV
                     EVLPEIENDHEIDLKPDDVRVDTYRSSGAGGQHVNKTDSAVRLTHLPTGVVVTCQNER
                     SQTQNRESAYKILRSRLLEMKLKEEAAQRAELKGDNIAAGFGSRIRSYVLHPYTQVTD
                     HRTETSVGDASAVLNGGLDPFIDSYLQYLITPAEATG"
     gene            complement(4176..5114)
                     /locus_tag="VGR29_08685"
     CDS             complement(4176..5114)
                     /locus_tag="VGR29_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV2073701.1"
                     /translation="MSNGTPENKASARTRQRRGGLGRGLESLIPTANPSTPAAASSGA
                     SDGTPREVPIEAISPNPWQPRAKMDPHKLEDLAQSIRTHGVMQPLVVTSSDEPDRYTL
                     IAGERRWRASGIAGLKTVPIIIKDVAPQAMLELAIVENVVRADLSPLEEAGAYRQLIE
                     DFGLTQADVAERVGRSRVSVTNTLRLLNAPDRVQAALANEQISEGHSRALLGLGSAAD
                     QLAVLDIVLEKGLSVRQTEDLVRRWLSGTPAEREKSAEHHPEDMRLESRLRDALGTKV
                     AVKRGTGKGGGTITIQYYSDEQLQALYDRLVGEDFW"
     gene            5302..6249
                     /locus_tag="VGR29_08690"
     CDS             5302..6249
                     /locus_tag="VGR29_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEV2073702.1"
                     /translation="MLNAIVGIIVLIVVLAATFGLINWAKRADEGDKAATMGLYLLFG
                     IPGALLTVAGSALLVNGKEGGWLALGLGLGLGFPLIRPFRKGLAQVTPMDPTSPIDFS
                     GLAIMLSVFAFLVYSLINAGGGEAAVPELSTGATISSLIINFLAFVGLAYIAVGYRNY
                     RNGAEATERLGLRTPTLMEVRIGLAAVVPAFIFSMIGSLLTSVFQPDAVDRLTETIDN
                     MTKGVQNPFGALLLGLCTGIGEEILFRGAIQPRFGILLTTLFWTALHIQYEVTWVIVG
                     LILMGIMLGLIRKYYGTTAAIITHAVYNALVVLIQMAFT"
     gene            6359..7993
                     /locus_tag="VGR29_08695"
     CDS             6359..7993
                     /locus_tag="VGR29_08695"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV2073703.1"
                     /translation="MKICEIRDLDGPNLFLWKPAIKLELEAEEGAIQVSRAASELAAG
                     EPVSVAELEPAEPERLMVLVVEVVNRLHDLCDQPRPDVAYRRMEEEGHYAVAYSWWHR
                     RFAMALAKLALQFILGETRDIDNGVASLKQILASPPEDDDAPQMVASRDRKTPIIAIT
                     GTNGKTTTSRLISFILRHAGKKVGMTSSAGVYIDTEPVLQGDYSGLGGARRVLESDVD
                     VAVLETARGGMLLRGLGYEECDVSVVTNVSADHLGLHGVLTVEGLAEVKSLVPRATRE
                     GGYAVLNADDPFVFAMREQIRARPFFISRRAISPEVRQHIENGGWALTLDDGQVHWWH
                     DGTSDVVTSLNGIPITFGGRAPHMVENALCAAAGCLGLGLGIDQVRTGLAEFRSSSRD
                     NHGRLNVYKLNGATVVIDFAHNEAGLRQLLEFAGHFRTGEGKMTAIVGTAGDRDEEAF
                     RAIGRHAALQADHVIKKDTTKYLRGRQPGDMLRLLQRGIDDAGTDVPVEDAPSEREAC
                     LRAFERVQSGDVIAVMCVEDYDFLLGYLDERGTPVN"
     gene            8135..9166
                     /gene="moaA"
                     /locus_tag="VGR29_08700"
     CDS             8135..9166
                     /gene="moaA"
                     /locus_tag="VGR29_08700"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEV2073704.1"
                     /translation="MATVSPLTLHPKKTAVSGPRDAYGRTMNYLRISLTDRCNFRCMY
                     CMPAVGMQFQPREELLTDDELVRLVGLFAETGVTKLRLTGGEPTIRPHLVDLVRQFKS
                     FPNIRELSMTTNALLLGRIAGDLKDAGLDRINVSIDTLDPVRFRFMTRGGRFDMVWAG
                     IEEADRVGLGPIKLNSVVLRGQNDHEVAEMAKLTIDRPWQVRFLEIMPMEGVGLVYDE
                     GLVTSEETRLELEKHFGPLEQLPAHPSDPARMWKIPGAQGTIGFISPMSEPFCDTCNR
                     VRLTADGNLRLCLLHPDELNLRDRLRSGATDAEIVATIRGGIWRKPWGHHVHERQMHT
                     VRGMSQIGG"
     gene            9295..10713
                     /locus_tag="VGR29_08705"
     CDS             9295..10713
                     /locus_tag="VGR29_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV2073705.1"
                     /translation="MAANAAPGFLEKQFKLSRLGTNVQTEILAGLTTFMVMSYIIAVN
                     PLTLTFNGELTVTQVATVTCLVAGVMSIIMGLYTNRAIAIAPGLGINAIVAFTLVGSM
                     GLSFPEAMGVVVTEGIIITILVLTGVRQFVLEVVPLILKKAISVGIGFFILLLGLRGA
                     GVLAFHSGDFESEVGTYTGRLDLAPLNSWPILVSMIGLLVIIVLMARNVRAAIFWGII
                     VSTVTAFLVPGDITSLPSDPLSGPDFSLVGQFSFGYWGELGILTSILVVLSLMLTDFF
                     DTMGTLIGVGAQANYLDENGELPDAEKPLLVDSVAAVAGGFVSSSSATSYIESAAGVS
                     VGGRSGLVVVVTGLLFLLALPFVSLVEAIPGAATAPALIVVGLLMMSVLSEDEGGVDR
                     TGTRRGIDFGNMEDSFPVALTMLVMPFTFNITYGIGAGFVSFVLIKVARGKASQVHPA
                     MYAIALLFLLYFLRWSLFGAEF"
     gene            10852..>11386
                     /locus_tag="VGR29_08710"
     CDS             10852..>11386
                     /locus_tag="VGR29_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2073706.1"
                     /translation="MIDRPELRKIVFSHQPKKGLLEAVRSAVPNASVVVAKGEELRSA
                     IPGAQLVIGGKIPEDGLRDAAELHWQHVPFAGVESVITPALIERGILLTNSRGVSAPN
                     MAEHLIAMMLAFARALPFFVKAQQERRWKPFDPEPTFFELTGQTVLLLGTGAIGKATA
                     ERLRPFGCTIIGARRTPQ"
BASE COUNT         2200 a         3216 c         3510 g         2460 t
ORIGIN      
        1 tccatcgcat cgcaaacatg gcacaccacc gatatgggca accagcagga aatgcttgaa
       61 ctcgcattgc cctccggtga gcacgttgag gtgaccttcg ccgaggcttc tcccgacgat
      121 tcgctggccg gggagggcca ggaccgcacc ggcaagatgg aagagctaat gcagcgggcg
      181 ctggagttct cggagggtaa tccgccgcac catccaggaa cgctaccgcg gttccctgtg
      241 ccatcagcct catatcgtga cacgattgcg gtgccaatga ctgtcctggc tgtggatgat
      301 gccggagctc gcggcctata tgcaccaccg cgtcaggtgg ggatcagcat aaaggacggg
      361 agtctcgttg gcgcggggga gtttcctgga tttgatccag agcattggcc cccggcgcgg
      421 ctcggtgact ggccgccgcc acagctcgcc ggaattcccc atgtgcaatt gcaagcgatg
      481 atcgcgcgtt tcagtgcctg ctggagtcgt gtgcttgatg cgtggttcgc caaggttgcc
      541 gaagctgacg atattcttcg ccaggatgtg cgagaaggat tacatcgtcg atcgattctt
      601 gacctgatgg agatgctcca atattacgac cgtctcaatc ctgtgtttgc gaaatggctg
      661 aacgacatgg ttggtgaaga tcaacatgat gaagagacgc ggtagcgatt cggttgggtt
      721 ttccttgttg acaatgttgc tacgcgttat accctggccg gctgagttgc gtcttcgcgg
      781 tgtggagtaa ccggttctga agcttcgtgc ggcggcccga agtgggtggg gctgatgatg
      841 tgatcctggc gacgtagcca ggcccccgta accgcccagc tatatgcggt gacgaccccg
      901 cccaccgcga tatcgatcac atagtgttcg cccatgtaga caagcgctgc tcccatcgaa
      961 agcgcataga gccaggatcc cagctcccat ccccgacctc ggcctttggc ggcgaagacc
     1021 agcaggaacg tcaccgcgaa gtggatcgaa ggcatcgccg cgatcgggtt cgactctcca
     1081 acgattttgt agcctgcctg gtaaagccct ccacccagct ccctggcgat cggctccatg
     1141 atccggagga ccacgggcgc tccgggagag tcaatcgatt ctggcgccat ccagggtgga
     1201 ttggacggga taatgaaata gatgagcagt cccagcatca ggagcatcgt cagcgcggat
     1261 aggtagtgct ggaaacgtcg ctggttcttc caccagagcc agacaacgac agcatgggga
     1321 acgatgaagt aggaccagtg gatgaaggtg aaataatagt cataccaatg gatggaaccg
     1381 ggatcatagt acgcggcctg gaggcgaatc gtcggtagct cgcccccgaa cagccacctt
     1441 tcgaattcga ccgccttggt attcaacgtc ttcgccagga tggtttcatc tgcaaatgaa
     1501 cggagaaagg tgaagacgaa ccagactgtc gcgtaaggca cgatgctggc gacgaaggaa
     1561 cgagcccttc cagtcggaac aaagatgact gcgagcaggg ctatcaggcc ccagatcagg
     1621 aaggatcggt tggtatacac ggagacgacg acgaggccga cgcctatggc aaggcggatc
     1681 cagagttccg gtgtcatacg gatccggaac cgtgatgtct tcgaactgtg ctctatgtgg
     1741 acagggacgg ggacgtcgtc ctgctctgtg ggtggatcag cctgcctggt cacgcgcgga
     1801 aaacggctcc ttcgttaggg ctgtcctgat ggtcaaaatc cgttggttct gcgcagagta
     1861 tacgggatgc acaggagccg cttaacccgc cgggagttgt ttagcggctc tccagccggt
     1921 accggtggag agcttttgac gtcatcgaaa agcgattctc ggccgaatca ggatctggtt
     1981 ttttcgccat gaatccccgg cggagctgct cggcaatcca tcgttcatcc agactatgat
     2041 cagccgccag tgcgttgaca tcattctcgg tccaggcgtt gtcgcgctcc ttgagggcgg
     2101 catagaagcg ctgacgattc tgctcggcag gggcggttgg accggtcacc aaacgctcga
     2161 caacgggtcc tccttcgagg aagcggatga tgtccgggat gtcgttctcg gatacgttgc
     2221 tgtagacgat gttgtcggga taaatgacga agtttgggcc gatgtcgcag agatcaattg
     2281 tgccgcacgt attgaggtgg atcctggtgt tcaggcgctt gcgcatgagc tcgaaccgga
     2341 agcgcgtcag tacctggttc gcccccttct gaatgcagtg acttcccgtg cagacgagca
     2401 catgacgttc ggtccagtac atgcgggtgc gtccttcctg aggcaacaac agtcttcatg
     2461 gccgcagtct atcaaaggag cgatgcccgt ctcgcgtcct cgactgacca ttgcggcggc
     2521 ttcgagaggc aaggctgtgc acggggcagc ggtagacaga tagccggagc agcgacgctc
     2581 ttggcctctg gacgacatat ccctttagct cttaaccaac aaagataacc gcgaacctaa
     2641 gcgatggaga ctgtgaatgg gcctcggatt gagcgatcgc ggtgcggtag ggtctacgct
     2701 actgagaact cttaggaact ttcctgagtg ggactatggc aagcgaatcg gccacgcccc
     2761 tccccgacag catcaggatg agcgttttct aaccgaggag cttcgctagt ctgtccgcgt
     2821 tatcccgttg cttcggcggg tgtgattaga tactgcaggt aggagtcgat gaacggatca
     2881 agaccgccgt ttagcacagc gctagcatcc ccaactgatg tctcagtgcg atgatcggtg
     2941 acctgcgtat atggatgcag cacgtaggag cggatccggc tgccaaaccc ggcggcgatg
     3001 ttgtcgccct tgagctcggc gcgctgggca gcctcctcct tcagcttcat ctcgaggagt
     3061 ctcgaacgca ggatcttata cgccgattcc cggttctggg tctggctgcg ctcgttttga
     3121 caggtcacca cgacgcccgt cggcaagtga gtcaaccgca cagccgaatc cgtcttgttc
     3181 acatgctgtc caccggcacc ggacgatcgg taggtgtcga cacggacatc gtccggtttg
     3241 agatcgattt catggtcgtt ctcgatctca ggcaagacct caaccagcgc gaaggcggta
     3301 tggcggcgat gggctgaatc gaacggagag aggcggacga gacgatgaga tccgccttcg
     3361 cccttgagca ggccgtacgc gttcgatccg cggacctcga tcgttgcgtt cttgatgccc
     3421 gcctcgtcac cttctgttgt gtcgagtagg tcagccttga acccccgctt gtccgcccag
     3481 cgcaggtaca tgcgagagag catgaaggtc cagtcttggg cgtcgacgcc gccttctcct
     3541 gagtgaatga tcaggatcgc gtcggaccgg tcgtgcggtt cattcaatgc cgactgcaac
     3601 tccatactgt cgactttttg caggaggctg tcacgctccg ctgagatttc ctcgatcagt
     3661 tcgggctcat cttccgccat ctcaagaaga tcgagcatgt ccgccactga ttgctccgcc
     3721 tgggtccagt tgtcgatgcg cgccttaatc tcaccgaggc gacgcatctc gttttgagcc
     3781 gtggtggggt cgttccagta ctccggatcg gagctccggc gttcgagatc ttcgatctct
     3841 cgctcatcgt tagcgaggtc aaagacgccc ccggagcgaa tcgagtcgtg tcgcgagttc
     3901 ggagagggat tttacgaggt catcgcgcga ggccatggat accaccttct gttgtttacg
     3961 tcatttcaag tgccggagac acatgagttc cagccgaatc tatccaatta tacgagggag
     4021 tttaggtgga caatgcaatg acagagaagg gagcgagggg tgatgcaggt cgggcgactc
     4081 gacggagcga tgagggaagg gaatgaaccc ttacgagcga gaactttgat agatgtcgtc
     4141 gcgcgtcccc gaacattggt tctcggaagg accttttacc agaagtcctc gccgaccagg
     4201 cggtcgtaga gcgcctgcag ttgctcatct gagtagtatt ggatggttat tgtgccacca
     4261 cctttacccg ttccccgttt gacggcgacc tttgtcccca gcgcatccct caggcgcgat
     4321 tcaagccgca tatcctctgg atgatgctcc gcgcttttct cccgctccgc tggcgtgccc
     4381 gaaagccatc ggcgaacgag gtcctcggtc tggcgcacgc tgagcccctt ctcaagtacg
     4441 atgtcgagca ccgcgagttg gtccgccgcc gagccaagtc cgagcagggc ccgcgagtga
     4501 ccttcggaga tttgctcgtt cgccaacgcg gcctgcacgc ggtccggtgc gttcaacaaa
     4561 cgcagcgtat tggtgacact cacacgtgag cgcccgactc gctcggccac atccgcctgg
     4621 gtgaggccaa aatcctcgat cagctgcctg tacgcgcccg cttcctccag agggctgaga
     4681 tcggcacgga cgacgttctc cacgatcgcg agctcgagca tcgcctgtgg cgcgacgtct
     4741 ttgataatga ttggtacggt cttcagccct gcaatgccgg atgcccgcca gcgcctctca
     4801 cccgcgatca acgtgtatct gtccggttcg tccgacgatg tcaccacaag tggctgcata
     4861 acgccatggg tgcgaatgga ctgtgccaaa tcctcgagtt tgtgcggatc catcttggcg
     4921 cggggctgcc agggattggg cgaaattgct tcgatcggca cttcgcgggg cgtcccgtcc
     4981 gaagcaccgg aactcgcggc ggcaggcgtg ctcgggttgg ctgtgggaat gagtgactcc
     5041 aggccgcggc ccaatccgcc gcgacgctgg cgggtgcgtg ccgatgcctt gttttcaggc
     5101 gtgccgttgc tcacttggat agggctcctc gctgcggcat gccgcggtcc tcgggcagga
     5161 ttctcacgcc ttgcaaccgt gccgcatgtg cggaaagtct agcattgccg cccggtatac
     5221 ttcctgccag cactggacat cccggccacg acgtggctga cgtcgtttcc caaagcccta
     5281 tctgaggtca tatacgcatc tatgctcaac gcaattgtcg gaatcattgt gctcatcgtg
     5341 gtactggcgg ccacgtttgg actgatcaac tgggcgaagc gggcagatga gggggacaag
     5401 gccgccacca tggggctcta cctgctcttc ggtatcccgg gggctttgct gactgttgct
     5461 ggttccgcgc tgctcgtgaa tggaaaggaa gggggttggc tggcgcttgg gcttggcctt
     5521 ggccttggat tccccctgat ccggccgttc cgtaagggcc tcgcccaagt gacgccaatg
     5581 gatccgacat cgcccatcga cttttccggg ctggcgatca tgctttccgt attcgccttc
     5641 ctcgtctatt ccctcatcaa tgctggtggg ggcgaagccg ccgtaccgga gctgagtacc
     5701 ggtgcgacta tctcttccct gatcatcaat tttctggcct tcgtcggact tgcgtatatc
     5761 gccgtgggat accggaatta ccggaacggt gcagaggcca ctgaacggct cggcttgcgg
     5821 acaccgacac taatggaggt gaggataggg cttgccgcag ttgtccctgc attcatcttc
     5881 agcatgattg gttcgctgct gacctcggtg ttccagcccg atgctgtaga ccgactgacc
     5941 gagaccatcg ataatatgac caagggggtg cagaaccctt tcggcgccct tctgctcggg
     6001 ctctgcaccg gtattggcga ggagatactt ttccgtggtg ctatccagcc gcggtttggt
     6061 attttgctca cgacgctgtt ctggaccgca ctccatatcc agtacgaggt cacatgggtc
     6121 atcgtcgggt tgatcctgat ggggatcatg cttggactca taaggaaata ttacgggaca
     6181 actgctgcca tcattacgca tgccgtgtat aacgctcttg tcgtgttaat ccagatggcg
     6241 ttcacctgaa tgcctgattc gcggcccccg tgttgcgtag cagacatact catgatccgg
     6301 cgttcgtgat gccctggaat gacccgtact cgcggagcaa gatgaccaga ggaagccgat
     6361 gaagatatgc gagattcgcg accttgatgg accgaacctg ttcctgtgga agcccgcgat
     6421 caaactggag cttgaagcgg aagaaggcgc aattcaggta agccgtgccg cgagcgagct
     6481 cgccgcgggg gaaccagtga gtgtcgcaga gctcgaaccg gccgagcccg agcgattgat
     6541 ggtgcttgtt gtagaggttg tgaaccggct ccacgatctt tgcgaccagc cccgccctga
     6601 cgtggcatac cgcaggatgg aagaggaggg ccactatgca gtggcctata gctggtggca
     6661 tcgccgcttt gccatggccc tcgctaagct ggcactccag ttcatcctcg gcgagacccg
     6721 ggacatcgac aatggggttg ccagtttgaa gcagattctt gccagtcctc ccgaggacga
     6781 tgacgcgcct caaatggtgg cgagccggga ccggaagacg ccgatcattg cgatcaccgg
     6841 aacgaacggg aagaccacca cctcccggct tatcagcttc atcttgaggc atgccgggaa
     6901 gaaggtggga atgacgtcgt ctgccggtgt ctatatcgat accgagccag tattgcaggg
     6961 cgactattcc ggactcggag gggcgcgacg tgtgcttgag tcggatgtcg atgtcgcggt
     7021 gctggagacg gcccgtggtg gcatgttgct acgcggcctc ggatacgagg agtgcgacgt
     7081 cagcgtcgtg acaaatgtca gcgccgatca tctggggctc cacggtgtac tgactgttga
     7141 aggtcttgcg gaggtcaaga gtctcgtacc gcgagcgacg cgggaaggcg gttatgcggt
     7201 cctcaacgcg gatgatccgt ttgtgttcgc catgcgcgag cagattcggg cccgaccgtt
     7261 ttttatcagt cggagagcaa tctccccgga ggtgcgccag cacatcgaga acggtggatg
     7321 ggccctcacg cttgatgacg gtcaggtgca ctggtggcat gacgggacgt cggatgtagt
     7381 gacatcgttg aacggtattc cgatcacctt cggcggtcgt gcgccgcata tggtggagaa
     7441 cgccctttgt gcggcggcgg gatgcctcgg gctcggcctg ggaattgatc aggtgcgcac
     7501 cggtctcgca gagtttcgca gcagttcccg ggataatcac ggtcgcctca atgtctacaa
     7561 gctgaatggg gcaacagtgg tgattgactt cgcccacaac gaggcaggtc tcaggcagct
     7621 tcttgagttc gccgggcact tccgtaccgg cgaggggaag atgaccgcta ttgtcggcac
     7681 cgcgggagac cgcgacgagg aagccttccg cgccatcggc cgccatgctg cactgcaggc
     7741 ggatcacgtc atcaaaaagg acacaaccaa gtatctgcgg ggtcgtcaac ctggtgacat
     7801 gttgaggttg ctgcagaggg gaatcgatga cgccgggact gatgtaccgg tcgaggatgc
     7861 cccctcggaa agggaagcgt gtctgcgcgc tttcgagcgg gtgcaatccg gcgatgtgat
     7921 cgctgtgatg tgcgtcgaag actatgattt cctgctcggt tacctggacg agcggggaac
     7981 gcccgtcaac tagcaactcc agcatccatg cgccgaagca ccggccttgt tgacgtgtgc
     8041 ttcggtactt gctaccctgc tttataccta gtcaaaggtc gaatagctac gcaaccggcg
     8101 tagcgggcac ctatgagtta aggaaacaac ccggatggcg actgtcagcc cgcttacact
     8161 ccaccccaag aagaccgcgg tttccggccc acgcgacgcg tatggccgaa caatgaacta
     8221 cttgcggatc tccctgacag atcgctgcaa ctttcgctgc atgtactgca tgccagcggt
     8281 cggaatgcag ttccagcccc gggaggaatt gctgaccgac gatgaattgg tccgtttggt
     8341 cggcctgttc gccgaaaccg gcgtcaccaa gcttcgcctg accggtggag agccgacgat
     8401 ccgtccgcat ctggtggatc tcgtcaggca gttcaaatcg ttcccgaaca tacgggaact
     8461 ttcgatgacg acgaacgcct tgctcctcgg ccgtatcgcc ggtgatctca aggacgcggg
     8521 tttggaccgc atcaacgtca gcatcgacac cctggaccct gtacggttca ggttcatgac
     8581 cagaggcggt cgcttcgata tggtctgggc tggaatcgag gaggcggacc gcgttgggct
     8641 tgggccaatc aagctgaaca gcgttgtcct caggggtcag aacgatcacg aggtcgccga
     8701 gatggcgaag ctgacaatcg atcgcccatg gcaggttcgg ttccttgaga tcatgccaat
     8761 ggaaggcgtg ggcctggtgt acgacgaagg actcgtgact tccgaagaga cacgcctgga
     8821 gttggagaag cacttcggcc cgcttgagca gctgcccgcg cacccgtcag atcctgcgcg
     8881 aatgtggaag ataccggggg cgcagggaac gattggtttc atatctccaa tgtcggagcc
     8941 attctgcgac acgtgcaacc gggtccgcct gacggcggat ggaaacctgc gtctttgctt
     9001 gttgcatccg gacgagctga acctgcgaga tcgcctccga tcgggcgcta cagacgccga
     9061 gatcgtcgca acaattcggg gcggaatctg gcggaagcca tgggggcatc atgtgcatga
     9121 acggcaaatg cacacagtgc ggggcatgtc ccagataggc ggctagcgct caacatgcaa
     9181 taacccgaaa accccttgta cgttcgcggc tttaccatca caatgaactc gggtggggcg
     9241 cgccgggcca ccggtgcgct cttcgttttc tctgaacagg ggaggggaac gtagatggcg
     9301 gcgaatgctg ctcctggctt tctggagaag cagttcaagc tctccaggct cgggacgaat
     9361 gttcagacag agatcctggc cggcttgacc acgttcatgg tcatgtcata catcattgcc
     9421 gtgaacccgt tgacgctcac cttcaatggc gaactcacgg tgacacaggt agcgacggta
     9481 acctgtctgg ttgccggcgt gatgtcgatc atcatgggcc tctatacgaa ccgcgcgatt
     9541 gcgattgcgc cgggactcgg catcaatgcg atcgtggcgt tcacgcttgt gggaagtatg
     9601 gggctctcct tccccgaggc catgggcgta gtggtcacgg aaggaatcat catcaccatc
     9661 ctggtgctga ccggcgtgcg tcagttcgtg cttgaggtcg tgccactgat cttgaagaag
     9721 gcgatcagcg ttgggatcgg cttcttcatt ctcctgctgg gactccgcgg cgcgggcgtc
     9781 cttgccttcc acagcgggga cttcgagtcg gaagtcggca cctacaccgg acgtctggac
     9841 cttgccccgc tcaacagttg gccgatcttg gtttcaatga ttggcctcct tgttatcatc
     9901 gtcctgatgg cgcggaacgt tagggccgct atcttctggg gcattatcgt ctcgactgtg
     9961 actgctttcc ttgtacccgg ggatatcaca tcgttgccca gcgatccctt gagcggtccc
    10021 gacttcagcc tggtcggaca gttcagcttc ggttattggg gcgagctcgg gatcctgacc
    10081 tcaatcctcg tcgttctcag tctgatgctc accgacttct tcgatacgat gggcaccctt
    10141 atcggcgttg gtgcccaggc caactatctg gatgaaaatg gcgagctgcc ggatgcagag
    10201 aagccgctcc tggttgactc cgtcgccgcg gttgctggag gttttgtgag ctcgtcctcg
    10261 gccacctcgt atattgaatc ggcagccggc gtgagtgtcg gtggtcgaag cggtcttgtg
    10321 gttgtcgtca ctggtcttct cttcctgctg gcgcttccct ttgtgtcgct cgttgaggcg
    10381 atacctggtg ctgccacggc accggcgctc attgtcgttg gtctcctgat gatgagcgtg
    10441 ctctccgaag atgagggcgg ggtggacaga accggtactc gccgcgggat cgatttcggg
    10501 aatatggagg atagcttccc ggtagcgctg acgatgctgg taatgccgtt caccttcaac
    10561 atcacctatg gcataggtgc cggcttcgtg agcttcgtgc tgatcaaggt ggcacgtggc
    10621 aaggcatcac aggtgcatcc ggcgatgtac gcaatcgcat tgctcttcct gctctacttc
    10681 ctccgctggt cacttttcgg tgcagaattc taggatccgc cggaaactgg cgaccaaacc
    10741 gacaagagat gaggcacgat tggtccgcct ccgcgcccgg gggcgagcgc catccacgct
    10801 gacgtccacc cagttcgctt acgccatctg ccgctggagg actatagatt catgattgat
    10861 cgaccggaac tgaggaagat cgtcttctcc caccagccga agaagggttt gcttgaggca
    10921 gtgcggtcgg ccgttccgaa tgcgtcagtg gtcgttgcga agggggaaga actgcgatca
    10981 gcaatcccgg gcgcgcagct cgtgatcggt ggcaagatcc cggaggacgg gctccgggac
    11041 gctgctgagc ttcattggca gcatgtgcca tttgcgggcg ttgagagcgt gattacaccg
    11101 gccctgatcg agcgggggat cttgctcacg aacagcaggg gagtaagtgc gccgaacatg
    11161 gccgagcatc tcatcgcgat gatgctcgcg tttgcgcggg cactgccgtt ctttgtcaag
    11221 gcacagcagg aacgccggtg gaaacccttc gaccctgagc cgacattctt cgagctcaca
    11281 ggccagacag tcctgttgct cggcacgggc gccatcggga aggcgacggc ggagcgcttg
    11341 cgtcccttcg gatgcacgat cattggtgcg cgacgaactc ctcaag
//
LOCUS       DASYWM010000214         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_365210, whole genome shotgun sequence.
ACCESSION   DASYWM010000214 DASYWM010000000
VERSION     DASYWM010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365210"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1605
                     /locus_tag="VGR29_08715"
     CDS             217..1605
                     /locus_tag="VGR29_08715"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV2073707.1"
                     /translation="MEDREAIKSALKYAPRFLEFQQPYMPFVGAQVAVRHDGELLLNH
                     AAGFADLETKTPLTVDHLFRIASHSKTFTGVACMQLVEQGKLRLDDEAKAHVPELAES
                     PMGGVTVRELLGHGSGMTRDGEDSTFWSLDRTFPDKEELLDTIRQHGKVLGPNEHFKY
                     SNIGYSLLGLIIESASGQSYREYVTRNIVDKLGLKDTGPDLNLDRAEEYAKGYSSRAH
                     GAERIEIEHIDTYAESSATGFFSTAMELTDYFQAHLDGDDRLLTDGSKRRMRQVQWTI
                     SDDTKYGLGLSISVVNGRTYYGHGGGYPGHITMSKLDLDRKLSISVFTNSNDGPALTL
                     CTGILRLIDLALEEKAKTEPKEEQTLETFEGRFATLWGITDVVNLGGRLYALNPALDN
                     PASEANELEVVSDTELKVVGDGGFGAYGETMRYTFNEDGSVDYIRGGGGAKLVPAERF
                     QLPEKVKRPTPA"
     gene            complement(1765..2175)
                     /locus_tag="VGR29_08720"
     CDS             complement(1765..2175)
                     /locus_tag="VGR29_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073708.1"
                     /translation="MKKWSVPAALVLVLIAAVFAYALPGSSSAAQNCDDWGNDTLDRI
                     EIGRQLLYPRERQEESTGSLQGDAQALFDIAQEQANSNPPDEAFNLNGDLVEGFSAGA
                     NALSGSGAAEAQIALAKGIIYNADLRVAYLLDGC"
     gene            complement(2191..>2523)
                     /locus_tag="VGR29_08725"
     CDS             complement(2191..>2523)
                     /locus_tag="VGR29_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073709.1"
                     /translation="QRAQFAQYLQQQAGGQVGGRMSHDPREMAGMVSQIQRQNPGGLA
                     SLFGGGGGGGGIGDALGGLLGGGGGGSFPGGGLGKVALGGIAAYAMKEMMGGGDDDDN
                     RNRRKRSI"
BASE COUNT          547 a          738 c          748 g          490 t
ORIGIN      
        1 gcatagacgc gtgtaccgcg ggggttggcc atggacggtc ccaggcccat gcaccgaacg
       61 agaacatctt cgtcgaggat tttgtcctcg gtatcaagca catcgccgcg atcatggacc
      121 gctttgcgag aagatagatc tgacaggttg aaccgacccg gatctttcag ggtccagagt
      181 cgatgatgac caaatcaagt ggaagggtcg aggaagttgg aagataggga agcaatcaag
      241 agcgcgctca agtatgctcc gcggtttctg gagttccaac agccttatat gcccttcgtg
      301 ggcgcgcagg tggcagtgcg gcatgacgga gagcttctcc tcaaccatgc agccggtttc
      361 gcggatctgg aaaccaagac gcccctgacg gtggatcacc tgttccggat cgcatctcac
      421 tccaagacct tcaccggtgt ggcctgcatg cagctggtgg aacaggggaa gctgcggctg
      481 gacgacgagg ccaaggcgca tgtgcctgag ctcgcagagt cgccgatggg cggggtgaca
      541 gtgagggagt tgctggggca tggctccggg atgacgagag acggagagga cagcaccttc
      601 tggtcgctcg atcgaacgtt ccctgacaag gaggagttgc tcgacacgat ccggcagcat
      661 gggaaggtgt tgggcccgaa tgaacatttc aaatactcga atatcgggta ttcgcttctc
      721 ggtctcatca tcgagagcgc atccgggcag tcgtaccgtg agtacgtcac tcgcaacatc
      781 gttgacaagc ttggtttgaa ggacaccggt cctgatctga acctggacag ggccgaagag
      841 tatgcaaagg gctattcaag ccgcgcacac ggagccgagc gcatcgagat cgagcatatc
      901 gacacctatg ccgagagttc cgcgaccggg tttttctcaa cggccatgga gctgacagac
      961 tactttcagg cccatctgga tggcgacgat cggttgctta cagatggatc aaagcggcgc
     1021 atgcgccagg tccagtggac gatctcggac gacacgaagt acggactcgg gctaagcatc
     1081 tccgtggtca atggccggac ctactacggt cacggtggcg gttatccagg ccatatcacc
     1141 atgtcgaagc tggacctcga ccgaaagctc tcgatatcgg ttttcaccaa cagcaacgac
     1201 ggtcccgctt tgacgctctg cacgggaatc ctgcgcctta tcgaccttgc cctggaggag
     1261 aaggcgaaga ccgagccgaa agaggagcag acactcgaaa ctttcgaggg acgcttcgcg
     1321 acgctgtggg gaatcacgga tgttgtgaac cttggtgggc gcctgtatgc cctgaatccc
     1381 gcgcttgata atccggcttc ggaagcgaat gaactggaag tggtgtccga taccgagctg
     1441 aaagtggtag gggatggagg ttttggagcg tatggggaaa ccatgcgcta tacattcaat
     1501 gaggatggca gcgtcgacta catcaggggt ggaggcggcg ccaagttggt tcctgccgaa
     1561 cgattccagc tcccggagaa ggttaagcgg cctacacccg cataaccaag taacgtctat
     1621 gccggcgtaa tgtccatctc acctggcgag atgatatgaa agagccgatc ccgcaatggg
     1681 accggctctt tcttacgatg tgctgctgca ttgtagagaa agaacggccc aaggctattt
     1741 ggttatattc gccgggcgcg ctccctagca gccatccaaa aggtatgcga cacgcaagtc
     1801 tgcgttatag atgatgccct tggcgagcgc gatctgtgct tcagccgcgc cactgccgga
     1861 aagtgcattc gcgcccgcgg agaagccttc aaccagatca ccgttcaggt tgaacgcctc
     1921 atccggtgga ttgctgtttg cctgctcttg ggcaatgtcg aagagagcct gggcatcacc
     1981 ctggagcgac cctgtcgact cctcctggcg ctcccgcggg taaagaagct gcctgccaat
     2041 ctcgatccga tcgagggtgt cattacccca atcatcgcaa ttctgggcgg cacttgagct
     2101 acccggcaac gcatatgcaa acacagcagc aatgaggacc aaaacgagcg ctgccggaac
     2161 cgaccacttc ttcatcaacg tacccttgtg ctaaatcgag cgtttacgtc ggttcctatt
     2221 gtcgtcatcg tccccgccac ccatcatttc cttcatggcg taggccgcga taccgccaag
     2281 ggccaccttg ccgagccccc cacccgggaa gctcccgcca ccgccgccac caagcagacc
     2341 gcccagcgca tcaccgatgc cgcctccgcc tccgccgccc ccaaagagcg aggccaaccc
     2401 gcccgggttc tggcgctgga tctgcgacac catgcccgcc atctcccgtg ggtcatggct
     2461 catccggccg ccgacctggc cgcccgcctg ctgctgcagg tactgggcga actgggcccg
     2521 ctg
//
LOCUS       DASYWM010000215         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_215672, whole genome shotgun sequence.
ACCESSION   DASYWM010000215 DASYWM010000000
VERSION     DASYWM010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215672"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VGR29_08730"
     CDS             <1..865
                     /locus_tag="VGR29_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073710.1"
                     /translation="GGTVTLYDAGDRTIVEFAVEGAGEEHPTHIHEGACGDLEPEPAF
                     LLDNINEDGESTTVVDISLGDLLAEDYAIDMHLASNQLGTLIACVDIDGEPELPAGAT
                     PSASPEASPAATPEATPSDGTGGIIAGQAPTPTEDATDGTGGATETQPVTQIPAATEV
                     PVKTETPVPTPAKTPVPTPTTAPAVATAPADDSDGTGGATTTVPQQAPAAVGDGTAGV
                     SGKGTPVETTTLPQQAGVGAALDWPDSPATAAMWASAVGSVILAGSAWVIRRGERQSI
                     DTPSRWTRVGI"
     gene            990..1628
                     /locus_tag="VGR29_08735"
     CDS             990..1628
                     /locus_tag="VGR29_08735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2073711.1"
                     /translation="MRLLLIRHGQSQGNVDAIIQGADDPLTDFGRSQAHAVGRFLHQR
                     HEVTRLYASPLARARETAEIIGSYIDLAPALEPGLAEINTGEAAGLTWTEWTLANPEL
                     AERLSARDPSFEESWVGGESGREFVDRIFVAYDRIVTQHLGTDDIVAVVSHGGPLAWI
                     SAHVHGDPKEQWPHDRAVFVNCSISELVIDEKGVHTIGAWNQTSHLEMIREA"
     gene            1691..2437
                     /locus_tag="VGR29_08740"
     CDS             1691..2437
                     /locus_tag="VGR29_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV2073712.1"
                     /translation="MRLQVLAVSDEVDQRIYSSSIRERMGDIDMIIGCGDLPASYLEF
                     LTDALHRPVYYVLGNHAEEITRTGVRGETRHPEGCTDVGFKVERDPLSGLIIAGLPGS
                     PRYSDQDPVQFTEFQMKMHMFRMAPRLIWNKLKYGRALDILVTHSPPRNVGDREDVAH
                     QGFQSMHRFVELTKPRYQLHGHIHLYDRTESNTHQLYDTEVINVFPYQRLDLEFDALG
                     EPMKTVTMSSDALRVEVDDERAEVNMRHDH"
     gene            2427..3395
                     /locus_tag="VGR29_08745"
     CDS             2427..3395
                     /locus_tag="VGR29_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073713.1"
                     /translation="MITDQINSDFDRARNKALVNDLVSVLSGRENRLLPFHEIRRRLN
                     PEGESYRGIQTVPISQIVGSMDRYQDFDRAFLPRKKITQGRWKNVDRAYYEDVDLPPV
                     QLYKVGDVYFVKDGNHRVSVARERGVEFIDAEVIEGHVRVPLSSQMSTMELLLQAEYA
                     EFLRHTDLDKLQPDHDIRPTQLGRYDEIWHHIELHRQWLSAIWHREATVHEAVNDWYT
                     YIYMPIVQVVRDRGVLREFPHLTEADIYLWVMKHRHVLEDEKGQDVGPVASAQDYADM
                     MTRPLSVGDRMRSLIAGSDTRRSPDAEEERERRRRRLRRWAKPRAR"
     gene            3628..4080
                     /locus_tag="VGR29_08750"
     CDS             3628..4080
                     /locus_tag="VGR29_08750"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV2073714.1"
                     /translation="MSSISASAEREIAAPDERVYRTLADYQQHHPRILPDAFSGFRVE
                     QGGIGTGTVIHFEVTVGGRTESYHQRIEETVPGRVLREVDFDGSRATTFTVTPAGDRC
                     HVRIETNRHTGGIRGLVERLVAPRLRRPLYEDELDRLDRYAREHSDEA"
BASE COUNT          845 a         1246 c         1310 g          786 t
ORIGIN      
        1 cggcggcacc gtgacgctct atgatgctgg cgacaggacc atcgtcgagt tcgctgtcga
       61 gggcgccggc gaagaacatc ccacgcatat tcatgagggc gcgtgcggtg acctggagcc
      121 ggagccggcc ttccttctgg ataacatcaa cgaggacggc gaaagcacca cggtggtcga
      181 tatctcgctg ggtgacttgc tggcggagga ttacgccatc gatatgcacc tggcatcgaa
      241 tcagttggga acgctgatcg cctgtgtaga tatcgatggt gagccagagc tacccgcggg
      301 tgccacacct tccgcatcgc ctgaggcatc gccagctgcc acaccggagg caacgccttc
      361 agatggcacc ggcggtatca tcgccggcca ggcaccaacg ccgaccgagg atgccactga
      421 cggtaccggg ggggcgacgg aaacccaacc tgtcacccaa atccctgccg cgacagaggt
      481 tcccgtcaag actgagacgc cggtacccac acctgcaaag acgccggtgc ccaccccgac
      541 gacggcaccc gccgtcgcta ccgcgccggc cgacgatagc gacggcactg gtggcgcgac
      601 gacgaccgtt ccgcaacagg cccctgcggc agtgggcgat ggcaccgccg gcgtctctgg
      661 taaggggacg ccggtggaga ccaccacgct gccgcagcag gccggtgtcg gcgcggcact
      721 tgattggcca gacagcccgg cgacggcggc catgtgggcc agcgcagtcg gctctgtcat
      781 cctcgctggc agcgcatggg ttatccggcg cggcgagcga caatctatcg acaccccttc
      841 ccgctggaca cgcgtgggca tctaactggc cagcccgcca gacccttccg tgaacagtga
      901 ttgatacaga aaacgggccc ggtggcaagc cgggcccgtt ttctgcagta ttggtatgaa
      961 tactgtctgc gtacacactg gagatcctta tgcgtttgct gcttattcgt catggtcaat
     1021 cccaggggaa cgtcgatgcc attatccagg gcgctgatga tccactaact gattttggtc
     1081 gctcccaggc gcatgctgtt ggccgatttc tccaccaacg tcacgaggtg acacgcctgt
     1141 acgccagccc actcgcgcgg gcgcgggaaa ccgcggagat catcggcagc tatatcgatc
     1201 ttgctccggc cctggagcca ggtctggcgg agatcaacac cggggaggct gccggcctaa
     1261 cgtggactga gtggaccctc gcaaacccgg aattggcgga gcgcctttcg gccagggatc
     1321 cttcatttga agagagctgg gtgggtggtg aaagtgggcg ggagttcgtc gaccgtatct
     1381 tcgtggccta cgaccggatc gttacccagc atctcggtac ggatgacatt gttgcggttg
     1441 tctcgcatgg cggtccgctt gcctggatct cggcacatgt ccacggcgat ccaaaggaac
     1501 agtggccaca cgatcgtgct gtgttcgtca actgctcaat cagtgagctg gtgatcgatg
     1561 agaagggggt ccacacgatc ggtgcctgga accagacgtc gcatctggag atgatccggg
     1621 aagcctgagg catcagctgc cgtatcatat tggtcaatga cgagtggcgt gacgaacgga
     1681 tagctccagc atgaggctcc aggtactcgc ggtttccgac gaggtcgatc aacgcattta
     1741 cagctcgtcg atccgtgagc ggatgggcga tatcgatatg atcatcggat gcggggacct
     1801 ccctgcgtcc taccttgaat tcctgacgga cgcgctgcat cgtccggtct attacgtgct
     1861 cggcaaccac gccgaggaga ttacccgcac cggtgttcgc ggtgagacac gtcatcccga
     1921 gggatgcaca gatgtgggct ttaaggtgga gcgcgaccct ttgagtggtc ttattattgc
     1981 cggacttcca gggtctcccc ggtattcgga tcaggatcca gtgcagttca ccgagttcca
     2041 gatgaagatg cacatgttca ggatggcccc ccgcctcatc tggaacaagt tgaagtacgg
     2101 acgggcactc gatatcctcg tcacgcattc gccgccgcgc aatgtcggtg accgtgagga
     2161 tgtcgcgcac caggggttcc agagcatgca ccggttcgtc gagctcacca aaccccgcta
     2221 ccagttacat gggcatatcc atctctacga tcgaaccgag agcaataccc atcagcttta
     2281 cgacaccgag gtcatcaacg tctttcccta ccaacgtctc gacctcgagt tcgatgccct
     2341 tggtgagccc atgaaaacgg tcaccatgtc ctccgatgcg ctgcgcgtcg aggtggacga
     2401 cgagagggcg gaggtgaaca tgcgccatga tcactgacca gatcaattcc gatttcgatc
     2461 gcgcccgcaa caaggcgttg gtcaacgatc ttgtttccgt cctcagtggc cgggaaaatc
     2521 gcctcctgcc attccatgag attcgcaggc gcctgaaccc ggaaggggag agctatcgag
     2581 ggatccagac ggttccaatc tcccagatcg tgggaagtat ggaccggtac caggatttcg
     2641 atcgcgcctt cctgccccgc aagaagatca cccaggggcg gtggaagaat gtcgaccgtg
     2701 cctactacga ggatgtggac ctgccaccgg ttcagctcta caaggtgggc gatgtctact
     2761 tcgtgaagga cgggaaccat cgcgtttccg tggcccggga gcgaggtgtc gaattcattg
     2821 atgccgaggt gatcgagggg catgtccggg tgccgctctc ctcccagatg tcgaccatgg
     2881 agctgttgct ccaggcagag tatgcggagt tcttgcggca caccgatctc gacaagctgc
     2941 agccggatca cgatatccgg ccgacccaac tggggcgcta cgacgagatc tggcatcata
     3001 ttgagttgca ccggcaatgg ctctcggcga tctggcatcg tgaagccacg gtgcatgagg
     3061 ccgtcaacga ttggtacacc tacatctata tgccgatcgt gcaggtcgtg cgcgaccggg
     3121 gagtgctacg ggagttcccg catctcaccg aggccgatat ctatctctgg gtcatgaagc
     3181 accggcatgt gctggaggat gagaagggcc aggatgtcgg cccggttgcc tcggcccagg
     3241 actacgccga catgatgacg cggcccctga gtgtcggcga ccgcatgcgg tcgctcatcg
     3301 ccggcagtga cacccggcgg tcaccggacg ccgaagaaga gcgggaacgc cgacgtcgcc
     3361 ggctgcgtcg ctgggccaag ccacgggcac gataagagaa tcgcgggaga tgccggatgc
     3421 gaccatacat cccctggttc cccaccgctc gcccgaatag cgtcatcagc ctgagcatcg
     3481 tgccagcgtc aatgaatgct gcagtgtcct ctgtatagga ctcaggtgat tgatcacgaa
     3541 tcggagcgtc acggttcagt ggcagtccct agcaaggttc cgagaacgcc ggataagaca
     3601 accagagtca gggccgagga gggattcatg agcagtattt cggcctccgc cgagagggag
     3661 atcgccgcac ccgacgagcg tgtctaccgt accctcgccg actatcagca gcaccatcct
     3721 cgcatcctgc cggacgcctt ttccggtttc agggttgagc agggagggat cggtacgggc
     3781 acggtcatcc atttcgaggt cacagtcggc ggacggacgg agtcctatca ccagcggatc
     3841 gaggagacgg taccaggccg ggtgctgcgc gaagtcgatt tcgacgggag cagggccacg
     3901 acctttactg tgacgcctgc cggagaccgg tgccacgtgc ggatcgaaac gaacaggcac
     3961 accggtggta tccggggact ggtggagcgg cttgtggcgc cgcggctgcg ccgaccgctc
     4021 tatgaggatg agcttgaccg actcgaccgg tatgctcgtg agcatagcga tgaggcgtga
     4081 cccggaccag gatacgtgtg gtcagaattg atatcgatta gagggagggc ccgagggcca
     4141 aattccgtcc gcaaaatgga gcaaaccgtc cccggttttt cggggac
//
LOCUS       DASYWM010000216         6184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_421918, whole genome shotgun sequence.
ACCESSION   DASYWM010000216 DASYWM010000000
VERSION     DASYWM010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421918"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1840
                     /gene="carB"
                     /locus_tag="VGR29_08755"
     CDS             <1..1840
                     /gene="carB"
                     /locus_tag="VGR29_08755"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV2073715.1"
                     /translation="VGMECTLGSEEITPEVLWSAKQMGFSDRQIALCAGKGTDVIRNL
                     RRDLGMSPVYKMVDTCAAEFEAQTPYFYSTYEIEDEAPPIEGDRAIVVGSGPIRIGQG
                     IEFDYCSVQAAHALQDAGFASIMVNSNPETVSTDFDASDRLYFEPLDAESVEEIMRHE
                     SGGDDANMPPMIVQFGGQTAINLAHPLSDAGAPILGSSLDSIDLAEDRERFEAFLRGL
                     DIPQPPGATVTSLNDAEAVADRIGYPVLVRPSYVLGGRAMEVVYGSDHLARYVRNQTH
                     LTPEHPILVDKYLVGKEVEVDAICDGEAVLIPGIMEHIERAGVHSGDSFAVYPGINLF
                     PAEVERIVEYTTRIAIGLEARGLINIQYVIHAGHLYVLEVNPRSSRTVPFLSKATGVP
                     MVKLATNIMLGKTLKEQGYEGGLWPRQPLTAVKAPVFSMAKLRGVEVHLGPEMKSTGE
                     VMGIDRTFASAFFKALIASGLALPDNGSILISLADEDKVEALPMISQLARMGFKLYAT
                     EGTAAMIERAGVPVQLVTKRIGRGSPDMLDVILNGTVDGVINTPGPANKEMLDGLEIR
                     RAAVERGIPCITSVDTARTVVDAMEKASSVYTVEPIQMYRRQSFGY"
     gene            1910..2692
                     /locus_tag="VGR29_08760"
     CDS             1910..2692
                     /locus_tag="VGR29_08760"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV2073716.1"
                     /translation="MREFQGIITKKHPVMGDSWLMTFTAPTEVLHAARAGQFVNVLPR
                     ERAVYDPLLRRPFSVYGVAPDEGLMTILVRPYGRGGGWIVDRNVGSEIDVMGLLGNTF
                     DITPRSTHLLMVGGGVGAAPLLMLAREAVARKLSVTYLLGAANAGALLEASEIPGEVE
                     YVVATEDGSAGYHGFVTDLVPDYLQWADQVFACGPGPMFESLRSVVLANRMGKRPSVQ
                     VSVERTMACGVGACLGCMVETKRGMKTSCVDGPVFDMDVVQW"
     gene            2686..3669
                     /locus_tag="VGR29_08765"
     CDS             2686..3669
                     /locus_tag="VGR29_08765"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV2073717.1"
                     /translation="MVTVEKQQYMADLTVDLAPSNPRELVLRNPVIPASGCFGYGLEY
                     SPMIDVQRLGAFVCKGITPKPRSGNPMPRITETPAGMLNAIGLQNPGIDGFIRKYPPT
                     WEHWRVPAIVNISAESVEDYAMMARKLDDVAGIAGIEVNISCPNIEAGGFCFGWDARM
                     SEAVTRAVREATTLPVIVKLSPGALDIMEIASAVEGAGADAVSLINTLVGMTIDPRTR
                     RSKIANLTGGLSGPAVKPVAVRMVYQVASVVKIPVVGIGGISGVQDALEFFMAGASAV
                     QVGTAVFSTPSMLTRLIDELGAWLDSNGCTSLHDIVGAANPASGAKGESTF"
     gene            3666..4265
                     /gene="pyrE"
                     /locus_tag="VGR29_08770"
     CDS             3666..4265
                     /gene="pyrE"
                     /locus_tag="VGR29_08770"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV2073718.1"
                     /translation="MNQPDLLQQLEASGAFKKGHFLYASGLHGDTYVEKFNLLRDPGA
                     TSEVCRHFTDRFRDERIDVVVGPTTGGILLAFETARQLGVAAAYAERVSGGESGREIR
                     RGTTFTPGSRVLVVDDIMTTGGSVRETLAGLENHPVDVVAVGVLVDRSAGETTFADVP
                     LIAIASRKFDAWPADACPLCKGGVALEKPGTTAQPGKTA"
     gene            complement(4278..4493)
                     /locus_tag="VGR29_08775"
     CDS             complement(4278..4493)
                     /locus_tag="VGR29_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073719.1"
                     /translation="MAGFSLGSHGDDPVQIVRTIARISQMLIELRDEYIDQQREDALD
                     QIERRLQELHELGVQLHARRERDDNLE"
     gene            complement(5291..>6184)
                     /locus_tag="VGR29_08780"
     CDS             complement(5291..>6184)
                     /locus_tag="VGR29_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV2073720.1"
                     /translation="IPQIVKVMEDRRTGDERPFHMPETCPSCGTPTHREPGVAMRYCT
                     NAACPAQLKERIHHFVSRAAMDIEGLGEKLADRFVDLGWIHDVSDIYHLDWEQIASLE
                     GLGRKSTDNLRESVEKSKEQPLWRLINGLGIRHVGERTATLLADRFGSLDALMSSSAG
                     EIGSIFGIGDIVAASVYDFAQEERNQALMHRLKEAGLKVAGLRDESNGVRPLEGYTLV
                     LTGRLDTMTRPQAEERLRQAGANVTSSVSKKTSAVIAGADAGSKADKAIQLKVPVLPE
                     SAIEALLSGIVPEEIQQRRLS"
BASE COUNT         1149 a         1808 c         2012 g         1215 t
ORIGIN      
        1 cgtggggatg gagtgcacgc tgggcagcga ggagattact cccgaggtcc tctggtccgc
       61 caagcaaatg ggattctccg accggcagat cgccttgtgt gcagggaagg gcaccgatgt
      121 gatccggaac ctgcgtcgcg atctgggcat gtcgccggtg tacaagatgg tcgacacctg
      181 cgccgctgag ttcgaggcac agacgccgta cttctactcg acctatgaga tagaggatga
      241 ggcgccgccg atcgaaggcg accgcgccat cgtcgttggg tcagggccga tccggattgg
      301 acaaggcatc gagttcgact actgttcggt gcaggctgca catgcgttgc aggatgcggg
      361 tttcgcctcg attatggtca actccaatcc ggagacggtc tccaccgact tcgatgcatc
      421 cgaccggctc tatttcgagc ccctggacgc ggagagcgtc gaggagatca tgcgccacga
      481 gtcaggtggg gatgacgcaa acatgccgcc gatgatcgtg cagttcggcg ggcagaccgc
      541 gattaatctt gcccatcccc tgagcgacgc cggtgcgccg atccttggaa gctcgctcga
      601 ttccattgat ctcgcagagg atcgggagcg tttcgaagcc ttcctccgtg gactggacat
      661 accgcagccg ccaggggcaa cggtgacgtc gctgaatgac gcggaagcgg tcgcagatcg
      721 catcggttac ccggtgctcg tccggccgag ctacgtgctc ggtggtcgtg cgatggaggt
      781 cgtctacggt tccgatcacc tggcacgata tgtccgtaac cagacgcatc tcaccccgga
      841 acacccaatc ctggtcgaca agtatctggt cggcaaggag gtcgaggtcg atgccatctg
      901 cgacggcgag gcggtgctga tcccagggat catggagcat atcgagcgcg cgggagtcca
      961 ctccggcgac tcctttgctg tctatcccgg aatcaatctc tttccggcgg aggtggagcg
     1021 gattgtcgag tacacgacgc ggatcgcgat tggtctcgag gcacggggac tgatcaacat
     1081 ccagtacgtc atccacgccg gtcatctcta cgtactggag gtgaaccccc gatcatcccg
     1141 gacagtccca ttcctgagta aggccacggg tgtgccgatg gtgaagctcg ccaccaatat
     1201 catgctgggg aagacgctga aggagcaggg gtatgagggc gggctctggc cgcggcagcc
     1261 gctcacggca gtgaaggcgc ccgtcttctc gatggccaag ctgcgcggtg tggaagtcca
     1321 cctcggaccg gagatgaaat cgaccggaga ggtcatgggc atcgaccgca cctttgcctc
     1381 agccttcttc aaggccctga tcgcgtccgg actggcgttg cccgataacg gatcgatcct
     1441 gatctccctg gcggatgagg acaaggtcga ggcgctgccg atgatctccc aactggcgcg
     1501 gatgggcttc aagctctatg cgaccgaggg caccgcggcg atgatcgagc gcgctggtgt
     1561 gccggttcag ctcgtaacga agcgtattgg ccgcggttcg ccggatatgc tcgatgtgat
     1621 cctgaacggt acggtggacg gggtcatcaa tacccctggc ccggccaaca aggagatgct
     1681 tgacgggctg gagatccggc gggccgcggt ggagcgcggt ataccctgca tcacctccgt
     1741 ggacaccgcg aggacggtcg tggacgcgat ggagaaggcc tcctccgtct acacggtcga
     1801 gccgatccag atgtaccgcc ggcaaagctt tgggtactag catgccgcag ctggcgagcg
     1861 ggagaagatg atgtctgcca tccgggaagg tcacgctgga ggagcgcgga tgcgcgagtt
     1921 ccagggaatc atcaccaaaa aacatccggt tatgggtgat tcctggctga tgacgttcac
     1981 cgcgcccact gaggttctgc acgcggctcg ggctgggcag tttgtcaatg ttctgccacg
     2041 ggaacgtgcc gtctacgacc ctctattgcg acgacccttt tctgtctacg gtgtcgcgcc
     2101 cgatgaaggg ctcatgacga tcctcgtgcg gccctatggc cgcgggggag gttggatcgt
     2161 cgaccgcaac gtcggatccg agatcgatgt gatgggactg cttggcaata cgttcgacat
     2221 tacccccagg agcacccatc tgctgatggt cggaggcggg gtgggagcgg cgccgctcct
     2281 gatgctggcg cgtgaggcgg ttgcccgaaa gttatccgtg acctatctgc tcggagccgc
     2341 aaacgccggc gcattgctgg aggcttcgga gattcctggc gaagtcgagt atgtcgtggc
     2401 gacggaggac ggtagcgccg ggtatcacgg attcgtgacg gatcttgtac ctgactatct
     2461 ccagtgggcc gatcaggtct tcgcgtgcgg gcccggtccg atgtttgagt cactccggag
     2521 cgtggtgctg gcgaaccgta tggggaagcg tccctcggtc caagtgagcg tggaacggac
     2581 catggcatgc ggggttggtg cgtgcctggg gtgcatggtg gagacgaagc ggggaatgaa
     2641 gacctcctgc gtcgacgggc ccgtgttcga catggatgtg gtgcaatggt gacggtcgag
     2701 aagcagcagt atatggcgga cctcaccgtt gatctcgcgc cgagcaatcc tcgtgagctt
     2761 gtcctccgga accctgtgat tcctgcatca ggctgcttcg ggtatgggtt ggaatacagc
     2821 cccatgatcg acgtgcagcg gctcggcgcc tttgtctgca agggaatcac cccgaagccg
     2881 cgttcgggga accccatgcc gcgtatcacg gagacgccgg cgggcatgtt gaacgccatc
     2941 gggctgcaga atcccggcat cgatgggttc atcaggaaat acccaccgac atgggagcac
     3001 tggcgcgtgc cggcgatcgt caacatctcc gcggagtccg ttgaggatta cgcgatgatg
     3061 gcgcggaagc tggatgatgt ggcaggcatc gcagggattg aagtgaacat ctcctgcccg
     3121 aacatcgagg cgggcgggtt ctgctttggc tgggacgcga ggatgtccga ggcagtcacg
     3181 cgagcggttc gagaggcaac cacgctacct gtgatcgtca agctcagtcc tggcgccctc
     3241 gacatcatgg agatcgcctc cgcggtggaa ggtgcgggtg ccgacgccgt ctccctgatc
     3301 aatacccttg tcgggatgac gattgacccg cgcacgcgac gctcgaaaat cgccaatctt
     3361 accggcggtc tctctggccc ggcggtgaag ccggtagcgg tccggatggt gtaccaggtc
     3421 gcatcggtgg tgaaaatccc ggtcgtgggc atcggcggga tctctggcgt gcaggatgcg
     3481 ctggaattct tcatggccgg cgcatcagcg gtacaggtcg gcaccgcggt attctcgaca
     3541 ccctcgatgt tgacgcggct cattgacgag cttggagcat ggctagacag caacggctgc
     3601 accagcctgc atgacattgt gggagcggcg aatccggcat ccggggccaa aggagaatcg
     3661 acattttgaa tcaaccggac ctgctgcaac aactcgaggc cagcggcgca ttcaagaaag
     3721 gacattttct ctacgcgtcg ggtctgcacg gggatactta cgtcgagaaa ttcaacctgc
     3781 tccgggatcc aggggcgaca tccgaagtgt gccggcattt caccgaccgg tttcgcgacg
     3841 agaggatcga tgtcgtggtc ggcccgacga ccggtggaat cctgctcgcc tttgagaccg
     3901 ctcgacaact tggagtggcc gccgcctatg cggagagggt ttccggcggg gagtccggca
     3961 gggagatccg gcgggggacg acattcaccc ctggttcaag agtactggtg gtcgatgaca
     4021 tcatgaccac tggtggctct gtacgggaga ccttggctgg acttgagaac catccggtgg
     4081 atgtggtcgc ggtcggagta ctggtggatc gtagcgccgg cgagacgacg tttgccgatg
     4141 tgccactaat cgccatcgca tccaggaagt ttgatgcctg gccagccgac gcctgtcccc
     4201 tctgcaaggg tggcgtggcg ctggagaaac ccggcacgac cgcccagccc gggaagaccg
     4261 cctgagcttt cgacaggtta ttccaggttg tcgtcacgct cacggcgggc gtggagctga
     4321 acgcccagtt cgtgaagttc ttgcaggcgc cgctcaatct ggtcgagcgc gtcttcccgc
     4381 tgctggtcga tgtactcgtc ccggagttcg atcagcatct gggagatgcg ggcgatcgtg
     4441 cgcacgatct ggacggggtc gtccccgtgg gaccccagag aaaaaccagc catccttata
     4501 cccttcaaac agcccgcaat atccgggagc gccccatcct aacctgcatc cgtcggtggt
     4561 gtacgtccat ttacaacaga gtaacacggc ttcacgcggt tggatcgctt ttgattctcc
     4621 gatcacaacc attgatattc ggcgcgatgc cagagggtgt gcccgaatgg ctggttctcc
     4681 cggctgcggc gagcgcccag ccgagccgac aggcggctgg ccgacattca aggatgccag
     4741 gcctccctat acacgaaacg tggccacctt ggcagccttg agctgttttc agagaggtgg
     4801 catgtctatc gcgtctgtcg tctgtagcga agggctcgct gccgcccggc tgaggtggta
     4861 tcaccaccga aaagcgatag cgggcttttt tcgccttgca ggctcagcca ggggggtcgc
     4921 tcccgctatt cgcctgaggg ctcacctctg ggacttgcgg cgcatgcaac gtgttcgtga
     4981 ccgcgatgca gcaatagcct gccctgagcc tgtcgaatgg gtcctcctga catgccacaa
     5041 ggggtgactt ggcatagcca atctcgcccc tacaccctcc gcctggcaga cctgcgtatc
     5101 aaccgcattg caaaccgctt caacggcgag ttcactccga tatcaggctg ccaagatggc
     5161 cagcggccaa attgttttgc gtggccatgt aaggggtgcc ctcgcgcgag gagaagaacc
     5221 tctctggaga ggagcttcac agttccgacg ggagcttccc ccatagcagg cgcaacgcct
     5281 gcaattaact ctacgagaga cggcgctgct gtatctcctc cgggacgatg ccggagagaa
     5341 gcgcctcgat agcggactct ggaagaacgg gaaccttcaa ctggatggcc ttgtctgcct
     5401 tgctcccggc gtccgccccg gcgatcacgg cggaggtctt cttggagacc gatgaggtga
     5461 cgttggcccc ggcctgccgg agcctctcct cggcctgcgg gcgggtcatg gtatccagtc
     5521 ttccggtcaa gacgagcgtg tagccctcca gtgggcgcac accgttgctc tcgtctcgta
     5581 gcccggcgac cttgagcccg gcttccttga ggcgatgcat gagcgcctgg ttacgctctt
     5641 cctgggcgaa gtcgtagaca ctggcggcga cgatatcgcc gataccgaag atactgccaa
     5701 tttcaccggc gctagaggac atcagggcat ccagcgagcc gaagcggtcc gcaagcagtg
     5761 tcgcggtgcg ttcaccaacg tgccgaattc ccagaccatt gatgaggcgc cacagcggct
     5821 gttccttgct cttctcgacg gattcacgca ggttgtccgt gctcttccga ccaagccctt
     5881 caaggctggc gatctgctcc caatcgaggt gatagatatc gctgacgtca tggatccagc
     5941 caagatcgac gaagcggtcg gcgagctttt caccgaggcc ctcgatgtcc atcgcagcgc
     6001 ggctgacgaa gtggtggatc cgttccttca gttgcgcggg gcaagctgca ttggtgcagt
     6061 aacgcatggc aacgcccggc tcccggtgtg tcggcgtgcc acaggaggga caggtctcgg
     6121 gcatgtggaa gggccgctcg tcgccggtgc ggcgatcctc catcaccttc acaatctggg
     6181 ggat
//
LOCUS       DASYWM010000217         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_46160, whole genome shotgun sequence.
ACCESSION   DASYWM010000217 DASYWM010000000
VERSION     DASYWM010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46160"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VGR29_08785"
     CDS             <1..878
                     /locus_tag="VGR29_08785"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEV2073721.1"
                     /translation="HDFAAVGTLLAGTKPAADLAILNSYDDRWAIHTHEHHKDFNYRE
                     HLLHYYRPLAGGNVTTDIISTDASLDGYKLVIAPTLHLLNEERVARLLEFVERGGHLV
                     LTVRSGLKDDLNALLPSRPPGPLTEAAGVEVEDYYALIDTVPVSGALFTGTSSIWAER
                     LRVRDEVDTEIIARFGPSNGWLDHQPAITAHAWGSGKVYVIGAWLDDDSQHALLARIV
                     KEAGVEPVMQTSSGVEARKRVDGQGKEVYIVVNHERTEQILGLPWPAHNHLTDREEVE
                     DMLLAPYGVAVLTRS"
     gene            1028..2200
                     /locus_tag="VGR29_08790"
     CDS             1028..2200
                     /locus_tag="VGR29_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEV2073722.1"
                     /translation="MKITEIKTHVLSTAWRNLTFVRVMTDEGLEGVGEVRMLNHTDAL
                     LGYLAEAGPNHILGHDPSDIEALVRKMYLNDYARAGEIAMSAIATIEIACWDIVGQAL
                     GQPVYKLLGGAVRDRIKAYANGWYTVERTPAEFHAAAKRVIDKGYRALKFDPFGAGFY
                     ELEHAERIQVLELVEAVRDAVGPETEVLIEMHGRFNPVTAIEMARELERFKPSWVEEP
                     VPPENLAALKKAADKISIPVATGERIHTRYDYREIFELQAADIIQPDITHFGGLLETK
                     KLAAWADAYYVLVAPHNVGGPVSIAAGLHFAASTPNFKIQEHFNDFAEDWVKTVAPGN
                     PEVIDGYFALPQGPGLGVKLDEDVLQEHPRQHVYFNLYAEDWHRRQVVAPAETEQR"
     gene            2514..>3119
                     /locus_tag="VGR29_08795"
     CDS             2514..>3119
                     /locus_tag="VGR29_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073723.1"
                     /translation="MAKIAMIGAGGVVFPMRLLGDLLSFPALQNTTFSLMDIDIERAQ
                     RTATFARELSEHYGFSATFDVTDDRRDALTGANYVIVTFQVGGLEAFAHDVDIPRKYG
                     VDQTVGDTLGPGGVFRFLRSAPAYAEIARDMHELCPDALLINYANPMAMACWYLSELG
                     VKTVGLCHSVQGTSRMLARQLQVPYEDVSFRCAGINHQAWFL"
BASE COUNT          644 a          941 c          950 g          584 t
ORIGIN      
        1 gccacgactt cgccgccgtg ggaaccctgc tcgctggcac caagccggct gcagacctcg
       61 ctatcctcaa ctcctacgac gatcgttggg caatccatac ccatgagcac cataaggact
      121 tcaactaccg cgagcacctg ctccactatt accgcccact tgctggagga aacgtcacca
      181 ctgacatcat ctcaacggac gcgtcgctcg acggctacaa actggtcatc gcgccaaccc
      241 tgcacctgct gaacgaggag cgggttgcac ggctgctgga atttgtcgaa cgaggcggcc
      301 acctcgtgct caccgtacgc tcgggcctaa aggacgacct caacgcactc ctcccctctc
      361 gccccccggg tcccctcacc gaggcggccg gcgtcgaggt tgaggactac tacgccctga
      421 ttgacacggt acctgtgtcc ggagcattgt tcactggcac ctcttccatc tgggccgagc
      481 ggctcagggt acgggatgag gtcgacaccg aaatcatcgc ccggtttggt ccatcaaacg
      541 gttggctgga tcaccagcct gcgatcacgg ctcacgcatg gggcagtggc aaggtctatg
      601 tcattggcgc ctggcttgac gatgattcgc agcatgcatt gctggcccgg atcgtgaagg
      661 aagccggtgt cgagcctgtc atgcagacgt cctccggtgt ggaagcccgg aagcgagtgg
      721 acggacaagg gaaagaggtc tatatcgtcg tgaaccatga gcgtacagag cagattttgg
      781 gactgccgtg gccggcccat aaccacctga ccgatagaga agaggttgag gacatgctcc
      841 tcgcaccgta cggagtcgcc gttctcacgc gctcctaacc ataacgagta gtggccaata
      901 cgccgcttcc gggacgagcg agacattcgc atggtgatgg aggaaggata tccctcgatc
      961 tgccagttgg gggcctgccg aaacagctgg ctttgcccgg gccagtgaat agtatatgga
     1021 ggagagaatg aagatcaccg aaatcaagac gcatgtattg agcacagcat ggcggaacct
     1081 gacgttcgtt cgggtgatga ctgacgaagg gctggagggc gtcggcgagg tgcggatgct
     1141 caaccatacc gatgcgctgc tgggctatct ggccgaggca ggcccaaacc atatcctggg
     1201 ccacgacccg tcggatatcg aagccctcgt gcggaagatg tatctcaacg attacgcccg
     1261 ggccggtgag atcgccatgt cggccatcgc gacgatcgag atcgcctgct gggacattgt
     1321 cggccaggca ctgggacaac cggtctacaa gcttctcggt ggcgcagtac gcgaccggat
     1381 caaggcctat gcgaacggct ggtacacggt ggagcgcacg cccgcggagt tccatgccgc
     1441 agccaaacgc gtgattgaca agggctaccg cgcactcaag ttcgatccct ttggcgcggg
     1501 cttctacgag ctcgagcacg cggagcgaat ccaggtgctt gaactggtcg aggccgtccg
     1561 cgatgccgtc gggcctgaga ccgaggtcct gatcgagatg cacggtcgct ttaacccggt
     1621 tacggcaatc gaaatggcgc gcgaactcga gcgattcaag ccctcgtggg tcgaggagcc
     1681 ggtgcccccg gaaaacctcg cggccctgaa gaaggccgcg gacaagatct ccatccccgt
     1741 tgcaaccggg gagcgcatcc atacccgcta tgactaccgg gagatctttg agctgcaggc
     1801 cgcagacatc atccagccgg acatcaccca cttcggtggc ctgttggaaa cgaagaagct
     1861 cgcggcctgg gcggatgcct actacgtgct ggtggcgccc cacaatgtcg gtgggccggt
     1921 ttccatcgcg gcagggctcc acttcgcggc ctccacgccc aactttaaga tccaggagca
     1981 cttcaatgac ttcgcggaag actgggtgaa gaccgtcgca cccggcaacc cggaggtcat
     2041 cgacggatat ttcgcgctgc cacaaggacc aggactcgga gtgaagctcg acgaggacgt
     2101 gctccaggag catccacgac agcacgtcta tttcaacctg tacgccgagg attggcatcg
     2161 ccgccaggtc gtggcacctg cggagaccga acagcgataa gcgctgacga aagcgcgtgt
     2221 atagacactc tgagcggcat gctgaccaag acgtcacatg ccgctccact attccgcagt
     2281 tgaggtagct gctgaaagca acggctgccc ggcatggtgg actggtcggc gagccgtgtt
     2341 gatgctgcat tttccggctc gcgtatctct gcagccagtg ctatctcatc tctggattct
     2401 cggctggagc aacggagggc cagatggacg catgacgcat attgataact gggaaacgtg
     2461 tccgctcggc aagcgcctga gatagaggcc aatagtaggg gagaggatcg atcatggcga
     2521 agatcgcaat gataggagcg ggcggggtcg tctttcccat gcgccttctg ggcgacctgc
     2581 tcagttttcc agcgctgcag aacacgacct tctcgctgat ggatatcgac atcgagcgcg
     2641 cacagcggac cgccaccttc gcacgcgaac tctctgagca ctatggattt tcagcaacct
     2701 tcgacgtgac cgacgatcgc cgggatgccc tcacaggtgc caattatgtg atcgtcacct
     2761 tccaggtcgg tggcctggag gcctttgccc atgacgtcga cattcccagg aagtacgggg
     2821 tggatcagac ggtgggagac acgctcggac caggcggcgt cttccgcttc ctgcgctcag
     2881 cgccggcgta cgccgagatc gcgcgggaca tgcacgagct gtgtccggat gcattgctga
     2941 tcaactatgc gaatcccatg gcgatggctt gctggtatct cagcgagctc ggtgtcaaga
     3001 cggtcgggct ctgccacagc gtccagggaa catcgcgcat gcttgcgcgc caacttcagg
     3061 ttccgtacga ggatgtcagc ttccgctgcg ccggcatcaa tcatcaggcg tggttcctt
//
LOCUS       DASYWM010000218         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_215760, whole genome shotgun sequence.
ACCESSION   DASYWM010000218 DASYWM010000000
VERSION     DASYWM010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215760"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VGR29_08800"
     CDS             complement(<1..968)
                     /locus_tag="VGR29_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073724.1"
                     /translation="MRQSISTRFGILVALMLVVLPLFGVVPVAAQDSGLQDDNTYESP
                     QFGYSVTWGEDWSVRAGDVSSDNGGYDEITLRGDDGTLWIQGEADEVTPADAVETRIS
                     LEGDEEDIVAQDLEADVPMTEMLVGRDKVLIEGYTLDDAVVVVVLSAREHDFDAALAA
                     VQDQVLLNGEQVLTDEEIEGSGEATTNEETDEPADDVATGEESEESTDEEVDETRNST
                     VTGGSYTGQLYDYALEFDEEIWEVTGEIDSDESDGLFLENETGRLTIWTWNGYGDDPV
                     DCLDGEAAYYAEDPTVEDWIPAEDANGDPIRNESGDYAYGVFTLTY"
     gene            complement(1093..1575)
                     /locus_tag="VGR29_08805"
     CDS             complement(1093..1575)
                     /locus_tag="VGR29_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2073725.1"
                     /translation="MSVQSRTEHSREQPFPPKGERQHPLVLVVNDTQEILDLFREILE
                     EEGYDVVLSSYGFQEVAGIQEIDPDLAILDFIMGGEEQGWQLLQKIRMNRETRDLPII
                     VCTGAIRLAKELEGHLTAKGVGLVLKPFDIDDLLVEVGKSLSRFVKSEQVNSMPIRGT
                     "
     gene            1848..3707
                     /locus_tag="VGR29_08810"
     CDS             1848..3707
                     /locus_tag="VGR29_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEV2073726.1"
                     /translation="MRGVFRFRGRTDDGRDDNQKIILSDRQHELLSEEQAILERLATA
                     LDAFPATEEDRSSLTHAVQQLGELFLLVIVGEFNAGKSAFINALVGAEVMPEGVTPTT
                     AVINLLRFGERQTETMLPDGVIQRTFPADFLDEITIVDTPGTNAIIREHEALTQSFVP
                     RSDLVLFVTSADRPFTESEREFMAEIREWGKKIVIILNKIDLLRDDQSRQQVIQFVRE
                     SVQRFLGFTPEIFPVSALLAQQAKSVGDRNPGERDRLWEASRFEALEQYVVETLDEEG
                     RIRLKLLNPLGISEHLTARYLKATDDRLAVLADDLTTIDNIERQLNLYREDMHRQFAY
                     HLTRVENIIHRMNDRGDEFFDETIRIGRVFDLMNSDRIKNEFDRKVVGDTERAIDQGI
                     DELIDWMVEQDLRVWEAVNEYIDRRRLNKYADELIGEVGGQFRYDRRALLESVSKRAQ
                     EEVDRYDSDAHARELSLSVRNAVATVALAEAGAVGLGALVVAAASTAVVDITGILAAS
                     ILAGVGLFVLPVKRRNAREDFRKRSAELEERLMSVMREQFEHELNRSVGRITDAIAPY
                     TRFVRSSQDRFEGLGSDLKAIDDDLRSLRHRIGGDNDRPNLPQNPEPETATAP"
BASE COUNT          727 a         1235 c         1020 g          732 t
ORIGIN      
        1 atataggtca gcgtgaagac gccatacgca tagtccccag actcgtttcg gatcggatca
       61 ccattggcgt cctcagccgg aatccagtcc tcgacggtgg gatcctcggc gtaataggcc
      121 gcctcgccat caaggcagtc aacaggatca tccccgtaac cgttccatgt ccagatggtc
      181 aaccttcccg tctcattctc cagaaagagc ccatcactct cgtcggaatc tatctccccg
      241 gtgacctccc agatctcctc gtcgaactca agcgcgtagt cgtagagctg accagtgtaa
      301 ctgccaccag tgaccgttga attcctggtc tcgtccactt cctcgtcagt tgactcttca
      361 ctctcttcgc cggttgcgac atcgtcggct ggctcgtctg tctcctcatt ggttgtggcc
      421 tcgccgctac cttcaatctc ctcatcggtc aggacctgct cgccgttcaa gagcacctgg
      481 tcctgcacgg ctgccagggc agcatcaaag tcgtgctccc gcgccgagag gaccaccacg
      541 acgactgcgt catcgagcgt gtatccctcg atgagcacct tgtcccgacc gaccagcatt
      601 tcggtcatgg gaacgtcggc ctccaggtcc tgcgcgacaa tatcctcctc gtcgccttcg
      661 agactgatcc gggtctcgac agcgtccgcg ggggtgacct catcggcctc accctggatc
      721 cagagcgtgc catcatcacc gcggagggta atctcgtcat agccaccatt gtcggaggac
      781 acgtccccgg cccggacact ccagtcctct ccccatgtca ccgagtagcc aaactgggga
      841 ctctcgtagg tgttatcgtc ctgcagccca ctgtcctggg cggccaccgg tacgacgcca
      901 aagagcggca gcacaaccag catgagggct accaggatcc cgaatcgcgt ggagatactc
      961 tgtcgcatgg tctgtctttc tttttctgtt tacagcccga aggcatgaga aacgcgtccc
     1021 gggaagcaag tgactttcat cccgttgcac actccaccga tgctctacca ggaaaaacga
     1081 ggttggtccg gttcaggttc ccctgatggg catgctgttc acctgttcgc tctttacgaa
     1141 ccggctcaag gacttgccga cctcaaccag gaggtcgtcg atgtcgaatg gcttcaacac
     1201 caaccccacc cccttggctg tcaggtgccc ttccagctcc ttggccagcc ggatcgcccc
     1261 cgtgcagaca atgatcggaa gatcccgggt ttcccggttc atcctgatct tttgcagcaa
     1321 ttgccacccc tgctcctcgc cgcccatgat gaaatcgaga atcgccagat ccggatcgat
     1381 ctcctggatc cccgcgacct cctgaaagcc ataggaggac aggacgacgt cgtacccctc
     1441 ttcctccaaa atctcccgga agaggtcaag gatctcctgc gtatcgttga caaccagaac
     1501 cagcgggtgc tggcgctcac ccttcggggg gaagggttgc tctcgagagt gttcggttcg
     1561 cgactgcacg ctcatggagg cgcccttcgt gcgagcgatt gagcagggac ctgaagcacg
     1621 ctcccgtgca acgaggcatc atggagccca ttctacccga agatcttctg acaacctgac
     1681 ctgcccgtga caccggtctg gctaccccat tttccttgta actggtggct tgcccggtta
     1741 aatctggcgg agaatagtac tcttgcagaa tgcagagcca cgtccggcca ggcgcatcgg
     1801 aacgatgcgg accgaagccc ggcaacgggc aacaggagta gacctagatg cgtggtgtct
     1861 tccgttttcg gggccgcacc gatgatggcc gcgacgacaa ccagaaaatc attctcagcg
     1921 atcgccagca cgagcttctc tccgaggagc aggcgattct tgagcgcctc gcgaccgcgc
     1981 tggatgcatt ccccgccacc gaggaggacc ggtcatcgct gacgcatgcc gtccagcaac
     2041 tcggcgagct gttcctgctg gtgatcgttg gcgagttcaa cgccggcaaa tcggccttca
     2101 tcaacgccct cgttggtgcc gaagtcatgc cggaaggtgt gacgcccacc acggccgtca
     2161 tcaacctgct ccgtttcggt gaacgccaga ccgagacgat gctgcccgac ggggtgattc
     2221 agcgcacctt cccggccgat ttcctggacg agatcaccat tgtcgatacg cccggcacca
     2281 acgcgatcat ccgggagcac gaagctctca cccagagctt cgtgcctcgc tccgaccttg
     2341 tcctcttcgt gacctccgcc gaccgccctt tcaccgagag cgagcgggag ttcatggcgg
     2401 agatccgcga atggggcaag aagatcgtca tcatcctgaa caagatcgat ctcctgcgcg
     2461 acgaccagtc gcggcagcag gtgatccagt tcgtccggga aagcgtccag cgcttcctgg
     2521 gcttcacgcc agagatcttt cccgtctccg cactgcttgc ccagcaggcg aaatccgtgg
     2581 gagaccgcaa ccccggcgaa cgagaccgac tctgggaggc gagccgcttc gaggcgctgg
     2641 agcaatacgt cgtcgagacg ctggacgaag agggtcggat ccggctcaag ctactcaacc
     2701 cgcttggcat ctccgaacac ctgaccgcgc gttacctcaa ggccacagac gaccgcctgg
     2761 ctgtgctcgc cgatgacctg accacgatcg acaatatcga gcgtcagctc aatctttacc
     2821 gcgaggatat gcaccgtcag ttcgcctatc acctgacgcg cgtcgagaac atcattcacc
     2881 gcatgaatga tcgaggtgac gagttctttg atgagacgat ccgtatcggc cgtgtcttcg
     2941 atctcatgaa ctcggaccgg atcaagaatg aattcgaccg caaggtcgtt ggcgacaccg
     3001 agcgcgcgat cgaccagggt atcgacgaac tcatcgactg gatggtggag caggatctcc
     3061 gcgtctggga agcggtcaac gaatacatcg accgacgccg gctcaacaag tacgccgacg
     3121 agctgattgg cgaggtaggc ggccagttcc gctacgatcg ccgggccctg ctggaatcgg
     3181 tctccaagcg ggcgcaagaa gaagtcgatc gctatgactc cgatgcccac gcccgtgagc
     3241 tctcgctctc tgtccgcaac gctgtcgcaa cggtcgccct cgccgaggcg ggcgcggttg
     3301 gcctcggggc ccttgttgtc gcagctgcca gtactgccgt cgtggacatc accggcatcc
     3361 tcgccgccag tatcctggcc ggtgtcggcc tcttcgtgct cccggtcaag cgacgcaatg
     3421 cccgggagga tttccgcaag cgctccgccg aactggagga gcgactgatg tccgtgatgc
     3481 gcgagcaatt cgagcacgag ctcaatcgct ccgttggccg cattaccgat gcgatcgcgc
     3541 cctacacccg cttcgtacgc tcctcgcagg accgctttga gggccttggc agtgacctga
     3601 aggccatcga cgacgatctc cgctcgctcc gccaccgtat cggcggcgac aacgatcgcc
     3661 caaaccttcc ccaaaacccg gagccagaga ccgctaccgc gccatagtgc ccgt
//
LOCUS       DASYWM010000219         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_534235, whole genome shotgun sequence.
ACCESSION   DASYWM010000219 DASYWM010000000
VERSION     DASYWM010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534235"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..160)
                     /locus_tag="VGR29_08815"
     CDS             complement(26..160)
                     /locus_tag="VGR29_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073727.1"
                     /translation="MMYIEAFNNYSMGYGAAIATILAGISIVFIFAYLRRMLKDELEY
                     "
     gene            159..275
                     /locus_tag="VGR29_08820"
     CDS             159..275
                     /locus_tag="VGR29_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2073728.1"
                     /translation="MMGQDVTSLDGKRGWANESAYCASKFGLTGLTQSLGES"
     gene            312..2576
                     /locus_tag="VGR29_08825"
     CDS             312..2576
                     /locus_tag="VGR29_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073729.1"
                     /translation="MSSDGGRISERRPKVRGEQSKLLRLSSRGDEHTLSRVPALGFRF
                     RRRPPFDTSLSPPRRSHFSFFALIATVLVYAAVRVLDIQRVVTIDEPVFLSITANFAN
                     AIARGDFAKTSQFLYPAVTVMWAGAIGFVTELPNYISDYPSQTDGLRNIHKPIREVGG
                     NPLQALVAARIAKIAFQAFYFGIALWLMNRLFGLAVTAVAAAFIIFDPFLVAHDQLLH
                     VDGVTGITAFVTMLAVAAADRRRTGWGLWVLAGVTAALCWLTRLTGLVLLPIMLLVIA
                     DGALVQYRQGTVSAKAALAKAAKTAAVVTGASLVTTILVWPALWVDPLVALSDTIEGW
                     RAGIESPHPWGLYFAGETVTGDPGLLFYVFVFLYKITPFTLVGLGVISLAFLFRFGSL
                     MPNRSWRPVVILAAFLIVYSLGMAAGARKFDRYILPNFLIFDLFAAIGVVGVARLLWT
                     ARHTFWKGISVLAVVGLVAGQIVSVQAQRPYMLDYYNPLLGGTEAAADMLMLGWGEGM
                     DQVAEVILAQPGGESSLVRLPIPATSLIYFLPETTRVGGLQHLERQGQPGLLAWANTD
                     YAVTYILQWKRNTFTDIGPYLADFDPISTVRIDGVDFARVYDLRRIPPPPWMIEPNDC
                     RWQFDGKLTFAAYGQHRLREGETLGAREHMIELVFRTDVASPVTAAYPLEATLHPKVE
                     GADPVSFTASLVPNPQSGLLAQAVATVQLPEGRTLGDYWLEVTVIDPASGRPLPAVQL
                     RTFRSSMSAGLPMC"
     gene            2886..3008
                     /locus_tag="VGR29_08830"
     CDS             2886..3008
                     /locus_tag="VGR29_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073730.1"
                     /translation="MMTGLGLTPEEMTRIVKRMRQILGLAEASRLKFDEVVRQP"
     gene            complement(3064..>3351)
                     /locus_tag="VGR29_08835"
     CDS             complement(3064..>3351)
                     /locus_tag="VGR29_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073731.1"
                     /translation="GKEGNELEAHRAGVISAAERQLEYHNSEFEDLVAKLQQIPTKVI
                     ADTTGYNPRTVRRLKQGEFRPSANRLSQILTLASRHGIIPRYDGIQSVSGN"
BASE COUNT          596 a         1006 c         1004 g          745 t
ORIGIN      
        1 gcccagttga cctcccgttg tcgccctagt attccagctc gtccttgagc atgcggcgca
       61 ggtaagcaaa gatgaagacg atgctgatgc ctgccaggat ggtcgcgatc gccgccccat
      121 accccatgct gtaattgttg aaggcctcga tatacatcat gatgggtcag gatgtcacga
      181 gtttggacgg caagcgtggg tgggccaacg agagcgccta ctgcgcctca aagttcgggc
      241 tgaccgggtt gacgcaatcc ctgggtgaga gctagcctgt cgtcaaacga cgttgccttg
      301 gggaattcga tatgagtagt gatggcgggc gcatcagcga gcgacgtccc aaggtgcgtg
      361 gtgagcagag taaactgctt cgtctctcca gtcgtggaga tgaacacaca ctgtcacggg
      421 tccccgcact cggctttcgg tttaggcgtc gtcccccctt cgacacgtcg ctctctcccc
      481 ctcgccgctc tcatttctcg tttttcgccc tgatcgctac cgtgctggtc tatgccgcgg
      541 ttcgagtgct cgatatccag cgtgttgtca caatcgatga acctgttttt ctttcgatca
      601 ccgccaactt tgccaatgca atagcccgcg gtgacttcgc caagacaagc caattcctct
      661 accccgccgt gacagttatg tgggctggcg cgattggttt cgtcacggag ctaccaaact
      721 acatcagcga ctatccaagt cagacagacg gcctccgtaa cattcacaag ccgatccgcg
      781 aggtcggtgg caatcccctt caggcgcttg tcgctgcaag gattgccaag atcgcgttcc
      841 aggcgttcta ctttgggatc gcgctatggc tcatgaaccg gctatttggt cttgcggtca
      901 ccgcggtggc ggccgcgttc atcatcttcg atccgttcct ggtcgctcac gatcagttgc
      961 tgcacgtgga cggcgtgacc ggcatcaccg cgtttgtcac catgctcgcg gtggcggccg
     1021 cggaccggcg ccgcaccggc tgggggctat gggtacttgc cggggtgacg gcggcgctgt
     1081 gttggctcac ccgcctcacg ggactcgtgc tgctcccgat catgctgttg gtgatcgccg
     1141 acggtgcgct cgtgcaatac cggcagggca cagtgagcgc gaaagcggca ttggcgaagg
     1201 cagcgaagac cgctgctgtc gttaccggtg cgagcctggt cacaacaatc ctggtctggc
     1261 cggcactctg ggtcgatcca cttgtcgccc tcagtgacac aatcgagggg tggcgtgcgg
     1321 ggatcgaatc cccacatcca tggggcctct acttcgcggg ggagacggtg accggagatc
     1381 ctggcctgct cttctacgta ttcgtgtttc tctacaagat cacgcctttt acgctggtcg
     1441 gcctcggcgt catctccttg gcatttctct tccgtttcgg atcactcatg ccgaatcgct
     1501 cgtggcgacc agtcgtgatc cttgctgcgt ttctcatcgt ctattcactt gggatggcgg
     1561 caggcgcgag gaagttcgac cggtacattc ttccaaactt cctcatcttt gatctcttcg
     1621 ccgcgatcgg tgtggttggg gttgcgcgtt tgttgtggac agcccgccat accttctgga
     1681 agggcatcag cgtactcgca gtcgtggggt tagtagctgg acagatcgtt tccgtgcagg
     1741 cgcaacgccc ttatatgctt gactactaca atccgctgct cgggggcact gaagccgctg
     1801 ccgatatgct gatgctgggt tggggggaag gtatggacca agtagcagag gtcatcctgg
     1861 cgcaaccagg aggggagagc tccttggtgc ggctgccaat cccggcgaca tcactgatat
     1921 atttcctacc ggagaccacc agagtgggcg ggctccaaca cctggaacga cagggccagc
     1981 caggcctcct tgcctgggcc aatacggatt acgcggtcac ctacattctc cagtggaagc
     2041 gcaacacgtt caccgacatc ggtccctacc tcgcggactt cgacccgatc tccacggtgc
     2101 ggatcgatgg tgtcgacttc gctcgagtct acgatcttcg ccggattccc ccacccccgt
     2161 ggatgattga gccaaatgac tgcaggtggc agttcgatgg caagctgacc ttcgccgcgt
     2221 atgggcaaca ccggctacgc gagggcgaaa cgctgggagc cagggagcat atgatcgaac
     2281 tcgtcttccg gacggatgtc gcgagcccgg tcaccgccgc ctaccccctg gaggcgacgc
     2341 ttcatccgaa agtcgagggg gccgatccgg tctcgtttac cgcctccctt gtgcccaacc
     2401 cacagtcagg attgctagct caggcagtcg cgaccgttca gctacccgag ggaaggacgc
     2461 tcggcgatta ctggctcgag gtcacggtga tcgatcctgc atcaggccgc ccgctcccgg
     2521 cggtccaact tcgaacgttc cggagcagca tgtctgctgg tctgccaatg tgctagatgg
     2581 gaggccagat atcgtgcggc tcatggatcg ctactcctcg ctgagcgcgc cagggaacgg
     2641 gatccatccg caacattctg cgtattggtc tcaatagctg cgattgaccg acgctcactc
     2701 cgggcggaac ggggtgtctt cccgacgtgc gccatcccct gggcctcgac gaagcggtcg
     2761 cctgcgccgg gctgttggca cggtcggtcg cggctgtcct ccacgtccgg gtcgctgggc
     2821 gcggtgtatg gggtctcaac gaaaaaggat tagtgcatcg gcctggttag gaggaggtga
     2881 acagcgtgat gaccgggcta gggctgacac ccgaggaaat gacgcggata gtgaaacgga
     2941 tgcgacaaat ccttggctta gcagaagcga gcaggctgaa gttcgacgag gtcgtccgcc
     3001 agccgtgacg gccgttacag gtattggttc actcagtttt gcgggtgctt atcgttcact
     3061 taatcaattc ccggaaactg actgaatgcc atcataacgc ggtatgatcc cgtgccgact
     3121 tgctagcgtg agtatctggg acagccgatt agcacttggc cggaattcgc cttgcttcaa
     3181 tcgcctgacg gtccgtgggt tgtaccccgt cgtgtcagca atcaccttcg ttggaatctg
     3241 ctgcagcttg gcaacgagat cctcgaactc cgagttgtgg tactccagtt gccgctcggc
     3301 cgcggagatc acgcccgccc ggtgcgcctc gagctcgttg ccctccttgc c
//
LOCUS       DASYWM010000220        20211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_121700, whole genome shotgun sequence.
ACCESSION   DASYWM010000220 DASYWM010000000
VERSION     DASYWM010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 20211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20211
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121700"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VGR29_08840"
     CDS             <1..268
                     /locus_tag="VGR29_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073732.1"
                     /translation="GGALEEGQTQVTPRDMATRLEETGPIQLAAHSDFGHTSVWTEPV
                     DGNTRCTFAPHGSMEPFPLIPPGESRQILVEIGMELAQGTAPAK"
     gene            complement(376..1248)
                     /locus_tag="VGR29_08845"
     CDS             complement(376..1248)
                     /locus_tag="VGR29_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2073733.1"
                     /translation="MSTRSERDGGAPRHRSPGRFVGSALVQLVLVAYGIIVVYPLLIM
                     LASSLKTTGEIFANPLSLIPETVRLVNYVDAWNQANFGTYFRNSIFVCAVSVALILFF
                     GSMAAYVLARFSFPGNRAIYLLFLAGFMIPIRLAIVPLFIMMRDLRLLDTLWSLILVY
                     VAGGMPFTIFLMVNFFRHIPRDLEDAAVLDGAGPFQVYYQIMLPLVRPALATVGLFHF
                     LSVWNDFFFPLIFIRSENLRTVPLGVSTFFGEYTNDWALMFAALSISIMPVIAVYLLA
                     SKQFIAGLTSGAIK"
     gene            complement(1238..2233)
                     /locus_tag="VGR29_08850"
     CDS             complement(1238..2233)
                     /locus_tag="VGR29_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2073734.1"
                     /translation="MRVFRFLRRPSVAADADTSLPGRRGRAVAIKQHTYLLMLVPGLA
                     MYLAFIIWPFANTIRVSFYNWSGLGPLDDFVGLGNYTNLFNRAPINRQFSNALSNTAQ
                     VFFLSTVLSTFVGLGFALILSRKIPGSRFYQALYFMPNTLSVVVVGFLWNLLLNPQFG
                     AVNAVLRSIGLDSLARPWLGDPDFALPSIVAVSAWANMGFPVLIFLAAILGIPSDLLE
                     AARLDGASELRVVATIVIPLLWPTLITLIVLNFIASVNTFELVFAMEGSEGGPFFATD
                     VLGTLFYRMAFGGVGGVYAGTGLGGAAALATLMLVIVLPVSVFVIIYQRKVSHEY"
     gene            complement(2299..3657)
                     /locus_tag="VGR29_08855"
     CDS             complement(2299..3657)
                     /locus_tag="VGR29_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2073735.1"
                     /translation="MSDIQKLLSQLHNNELSRRQFITRAVGLGLSASAAVSLATGGRA
                     AAAIQATPAADREPVTVTIWSWREDDVLVWQAAAEDLQETWPGLEVEVEVSDATEFGT
                     KVSTAMQGGQGPDIITTRAGEGYFRPYAEANMFVPLTDKVPGLAEIPEGTLGQVSFGD
                     EVLAVPFASQVWVFYYNTQIFEEHGLTPPETWSDLMSTFQTLKDAGVTPLFVPAREGW
                     VLSGYVDCIGSTYLGDEFAGQLIAGEKQFTDEKFVSLLSRIKDLQPFFQEGYVGNNVP
                     DMDAAFQSGQAAMILYGGWAANTYIEQAPDLEFDFFLSPPDEAGEEPSSYVFVDGGYA
                     VNAASEVQEAALELVKYSATVDYGQTFSDITQEMSSIPGVTATEENKYLQRQIEFAEH
                     AIQNLYRIRSPFDQGDPGISTLLHPLMQGLLSDQVTPEEVAQQLQEGLAQWYEPFQNG
                     QG"
     gene            complement(4181..6004)
                     /locus_tag="VGR29_08860"
     CDS             complement(4181..6004)
                     /locus_tag="VGR29_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2073736.1"
                     /translation="MTTASTYTSFKGRLRMFALAGILAVSAMFPAVSGTLASGDDVVT
                     PTAVVSTDDQFDISGADGLIGLTARVVADGDGANLRAAAEHDAEVVATVPDGTIVDLR
                     VDAVDTVYDPDGVTRWWPVSFNGLDGWIVGFYLTDPDTPVPSDGTAGTTSTTSAPSRT
                     PYEYTGSMTAEISADGDGLVLRVEPDSNSAKVASLSEGTIVELRIDELDTVYDEQGTR
                     WWPVAVDGLEGWVSGFYLIDPGTAGTQPAPTVTIPTTLDPPSADPTDGVFLAGEWAEI
                     RTSDGSGVNLRAGASANADQSGFAPNQALVEILGGPENGWYQVRWDRQVGYVDGSLLI
                     PANPPMLASEARASDTEAASVETAAIDAPDTVGTLTEGDFAMVDAGSDVGINIREEPT
                     QESERAGFLTEGSVVTVTEGPLADAEGVAWYRVTDGGQSGWARGDLLVPTEAPAQVED
                     VTAPAETDTEGETATEDAATGFILPLSRFTFTQDYGCSSLGFYTYNPTFGCSVHDGVD
                     LAAPSGTPIQAAEGGTVVASGWCDCGLGYYVEIDHGSGLHSVYGHMASQPYVAVGQTV
                     AQGDVIGPVGSTGLSTGPHMHFMVRQDGVTQDPKNYLPPLS"
     gene            6834..8312
                     /gene="tig"
                     /locus_tag="VGR29_08865"
     CDS             6834..8312
                     /gene="tig"
                     /locus_tag="VGR29_08865"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV2073737.1"
                     /translation="MKLTTERKPGSLVELNITAEDKEFQEALDKAISRQARNLQVPGF
                     RKGKAPRRMVESYYGREAFLRDAADEVMDRLYRQALEQEDIRPVGEPDVEIVDLEPVN
                     FVVTVPVYPSFELGDYTAVRQEPIDATVTDEDVDEVLKRLQTQQSSWAELSEERTPRE
                     GDQITIDYSVKEGDQDFQEPVTDAVWVLGETNLLQQLRDKIEEMHVGDTEEFDLVFEE
                     DDETADPQIRGKELKYSVTLKGLKERQLSEIDDEFAKTVADAESVEDLKQQIREDIHQ
                     GKTNDARNEVLNAIVDQIAEQTDLDLPEAMIDEEVEHQLGHRKQEMQQQGMQWEQVLR
                     MSNTTEEAVKADMRPDATRRLRNSMLLQEIAKRENIGVSEQDLDAEIDRVAGADLNPD
                     VDDEEAKARAARLREVYNSDYFRNVLRNDMFEKKLTDRIIEIATEGRGAVLNGWTAPD
                     PASTSGTDETAEPASETPSNEAAPDEVVTTAEASTANEISDQ"
     gene            8423..9622
                     /locus_tag="VGR29_08870"
     CDS             8423..9622
                     /locus_tag="VGR29_08870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2073738.1"
                     /translation="MSANRTPDDLARYMWLLSPEGQDLLDRIREMTAKGTSDLRIAEH
                     LRSSYPADRVALAMTQHDLRLKARDKFAQADRLFFTREGLEQATSDRIAAHRADRFAS
                     TGRVIDLCSGVGGDLMALAGLPGIHHLAAVDLDPVHLLLAGANVAAVAPESEPRLIHA
                     DVRQVDLSAFDGVFIDPARRSTSGRFGGMGSEPPLEWALCLTDHVPAVGIKTAPGIPH
                     DLVPEGWEIETISLGPDIKEAVLWSPALASEPATATVIAGDAVHQLRYILGSVVPMRR
                     PEAGQWLLDPNPAVTRAGLVEDLARLLHAARIDKEIAFLISDSPVETPFARSLPVIDA
                     LPWHEKRIRERVRELDGGPVDIRRRGLAGDVETITRRLRGKGSRRLVIAMTRVKDEPW
                     AVICDIA"
     gene            9781..10425
                     /locus_tag="VGR29_08875"
     CDS             9781..10425
                     /locus_tag="VGR29_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEV2073739.1"
                     /translation="MEYPDIHNLVPMVVESTNRGERAFDIYSRLLKDRIIFLGTPVES
                     QMANLIIAQLLFLDHDDPEADISIYINSPGGEVYSGLAIYDTMQMIRPDIQTYCVGMG
                     ASMAAVLLAGGTAGKRFALPNSRIMIHQGSGGFRGNIPDLEVQAKETFSVINKLTEIL
                     AKHSGQPFDKVKADTQRDYYMTGEDAMNYGLVDKVLTPAAIDLSKIGKNGSSED"
     gene            10526..11773
                     /gene="clpX"
                     /locus_tag="VGR29_08880"
     CDS             10526..11773
                     /gene="clpX"
                     /locus_tag="VGR29_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV2073740.1"
                     /translation="MHYRCSFCNKGQEEVRRLIAGPNQVYICDECVQLCREIIEEEEP
                     STPKVEPVATNIPTPKALYEHLNNYIIGQDRAKKILSVAVYNHYKRINAGVQEADGVE
                     LQKSNVLLVGPTGSGKTYLAQTLARILDVPFSIADATALTEAGYVGEDVENILLRLIQ
                     NAGDVARAQRGIIYIDEIDKIARKSDNPSITRDVSGEGVQQALLKIIEGTVANVPPQS
                     GRKHPHQEYIQIDTKNILFICGGAFEGLEEIVSKRIGKQSLMGFGHEAEEAAAKIDNP
                     LSQMTHDDLMKYGLIPEFVGRLPVSVSLDHLTRKELMQILVEPKNAVVKQYQKFFALD
                     DVELMFTEDALEAAAAQAEARKTGARGLRTTIEEVLLEVMYEIPSLENVRKCIVDANV
                     ITNRSRPLLLSVNEQPIEYAQTA"
     gene            complement(11826..12506)
                     /locus_tag="VGR29_08885"
                     /pseudo
     CDS             complement(11826..12506)
                     /locus_tag="VGR29_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024626.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
     gene            complement(12519..12986)
                     /locus_tag="VGR29_08890"
     CDS             complement(12519..12986)
                     /locus_tag="VGR29_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073741.1"
                     /translation="MKRDDKAPARGKPRFVPGMELSRALFDEVIQPSLTRTMPGLRYA
                     AGLFGVGSEALGYDTARSMDHDWGPRCVLVLDKEDELVFGDRTREAIADAMPPVFRGF
                     PALFQDASDEPGAVVPADPARDAPTRHLVDVTTMDALHARVDYAPGDEADPVF"
     gene            13128..13400
                     /locus_tag="VGR29_08895"
     CDS             13128..13400
                     /locus_tag="VGR29_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV2073742.1"
                     /translation="MTNEPVRDVQIRVTGHVQGVGFRVGARHRARELGVHLHAENQPD
                     GSVLLEAKGNADAVERLVVWAKQGPRNARVDHVEVTNRDAGADGFA"
     gene            13493..14434
                     /locus_tag="VGR29_08900"
     CDS             13493..14434
                     /locus_tag="VGR29_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2073743.1"
                     /translation="MNPDPLARRPFLDYDVPPIAVGCAPLGDMPDTFDYSVPPEIAQA
                     TVRAALASPLNWLDTAASYGDGESERRIGKVLRELGGLPDGAFLDTKVGASPDGDYSA
                     ETVKRRFARSQELLGMERLEMVFLHDPEKVSYEEIAAPDGPLAVLQGYREEGLIRHLG
                     VAGGPIDLMIRYVETGIFDAVITHNRYTLLDQSADPLLDLCHVRNIPVLNAAPYGSGM
                     LAKGPSLYPRYAYSRATDDMIDRGRQLEAICSRHNVPLAAVALQFSMRDPRVTATIVG
                     LSRPERIEQTIDLATGEIPDTCWDEVLEVATGSRSTG"
     gene            complement(14495..15001)
                     /locus_tag="VGR29_08905"
     CDS             complement(14495..15001)
                     /locus_tag="VGR29_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2073744.1"
                     /translation="MVLENQGLFVPPESGRSVSLGGLEAIFKVPGAQTAGAYAVVEHV
                     LLPGQLGSPPHTHANEDEVSILLEGEIRVLIGEDVFTAGAGAYVVKPRGLPHAFWNAT
                     SVPARFVEIVSPPGFEQYFVEVADLLSAAGPPDMGGLARIAGRYGLTMHLERLPELME
                     RFNVRLGP"
     gene            15481..16923
                     /locus_tag="VGR29_08910"
     CDS             15481..16923
                     /locus_tag="VGR29_08910"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV2073745.1"
                     /translation="MRVLKFGGTSVGNARRVRESARIALNTPGTNVIVVSASGGVTNL
                     LIDAARAAARHDRDAVASAVAEIHARHENVLSGIGEDQLRATASGAVNQIHGSLTSTL
                     DEVFTQGQLSPKLSDKVVSHGEKAMALMMAAMVRDMGTPAEPVFTDTVIATDGRHSAA
                     RPDREQTRAAAASKILPLLQSGTTAVATGFIGFGPDGATTTLGRSGSDYSATLLGAAL
                     DAEEVQIWTDVPGVLSADPRKVPAARVISEISYDEAQELAHFGAKVLHPRTIRPAVAQ
                     NIPVRILSTFQPTNSGTVVTSRALAQGIKATTALKGLTLLTLDVPELEDLAPAASVIF
                     RVLHEQRVEILTVSQSSSRRRMSFIVDTVGGGCEMLTAQITAALDDIEADISCSDNVA
                     VVAAVGDGAANLPSSLARMLAVLHRAGIEVLATNQQNSNAAMVVVVPEDVSDHAVQVV
                     HDAFIGPSRRSNRSASRRRSDLLGESLRVG"
     gene            16955..18016
                     /gene="asd"
                     /locus_tag="VGR29_08915"
     CDS             16955..18016
                     /gene="asd"
                     /locus_tag="VGR29_08915"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV2073746.1"
                     /translation="MTDGRIPVAILGATGNVGQRFVQLLQDHPWFRVAELVASERSAG
                     KSYRDATDWRLGACMPEGVTELTVQDYNADLQSPLAFSGLPGEVAGDIEQRLAKNGLA
                     VLSNTSTHRMEPDVPLLIPEVNADHVAAIEVQKANRGWSGFIATNPNCSTIHLVLALK
                     PLHDAFGLDSVLVTTLQAVSGAGYPGVPSLDMIDNIVPYIGGEEEKIETEAMKLLGGY
                     EPDQGFIPDSFTLSAHCNRVPVRDGHTECASVGLTQPSTPAEAIEVFESFRGVPQELG
                     LPSAPERPVIVRYEDNRPQPALDRDDSNGMASVVGRVRECPVLGLKFVVLGHNTVRGA
                     AGASILNAELFCEKGILPI"
     gene            complement(18680..18883)
                     /locus_tag="VGR29_08920"
     CDS             complement(18680..18883)
                     /locus_tag="VGR29_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073747.1"
                     /translation="MNVRHEIDELASALLPPYEQRKDWPADAQATTDRLFAIIVGMYM
                     ANQLTSDQLGKLVHQLDGQERSS"
     gene            complement(19028..19486)
                     /locus_tag="VGR29_08925"
     CDS             complement(19028..19486)
                     /locus_tag="VGR29_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2073748.1"
                     /translation="MTQDRSASFEARASVVYGTDYQPHILALDDSPDILSLYQEVLEG
                     EQYHVSTGTFANHDVDQIAMMRPDLVILDFIQGGDQVPLRAVEALRRDSRIGGIPVIL
                     CTGALRQVAPIRPQLKALGVQVISKPFNIDALLEAVTSLLAPVPSAGQHS"
BASE COUNT         4233 a         6347 c         5931 g         3700 t
ORIGIN      
        1 gggaggagca ctggaggagg gccagaccca agttaccccc cgggacatgg ctacaagact
       61 ggaggaaacc ggccctatcc agcttgcagc gcactccgat ttcgggcaca cctcagtatg
      121 gacggagccg gtcgacggaa acacgagatg cacgttcgcg cctcacggat cgatggagcc
      181 cttcccgctc attcctcccg gcgagtcccg gcagatactc gtggagatcg gcatggaact
      241 cgctcaaggc accgcgcctg caaaatgact cccactctgt tccgcaccga ctgcgccggt
      301 cattcccgat tcaggttcgt agtgcttcgc cccgcaaacc gacctcccca acaaatggac
      361 cccactgctt cccgatcact tgatcgcacc ggaggtgagc ccggcaatga actgcttcga
      421 cgccaggagg tagaccgcaa tcaccggcat gatcgatatg ctcagtgcgg cgaacatcag
      481 cgcccaatcg ttggtgtatt cgccgaagaa ggtactcacg ccaagcggga cggtccggag
      541 attctctgac cggatgaaga tgagcgggaa gaagaaatcg ttccagacgc tgaggaaatg
      601 aaacagccca accgtcgcca gtgccggacg caccaggggc aacatgatct ggtagtacac
      661 ctgaaagggc ccggctccat cgagcaccgc ggcgtcctca agatccctgg gtatgtgcct
      721 gaaaaagttg accatcagga agatcgtgaa cggcatgccg cccgcgacgt agaccaggat
      781 tagcgaccac agcgtatcca gcagccgaag gtcgcgcatc atgatgaaga gcggcacgat
      841 tgcaagccgg atcgggatca tgaaccccgc caggaagaga agatagattg cccggttccc
      901 gggaaaggag aagcgcgcga ggacgtaggc ggccatggag ccgaagaaca ggatcagtgc
      961 gaccgagaca gcgcagacga agatgctgtt cctgaagtat gtcccgaagt tggcctggtt
     1021 ccatgcatcc acgtagttga cgaggcgaac cgtctcgggg atcaggctca aggggttcgc
     1081 aaagatctcc cccgtcgtct tcagcgatga cgccagcatg atcaagagcg gatagacaac
     1141 gatgatcccg tacgcaacaa ggaccagttg gacgagggca cttcccacga atctccccgg
     1201 tgagcggtga cggggcgccc cgccgtctcg ctccgatcta gtactcatgg gaaacctttc
     1261 gctggtagat gatgacgaag accgaaacag gcaagacgat cacgagcatc aacgtcgcga
     1321 gcgccgccgc cccgccgaga cctgtcccgg cgtagactcc acctactccc ccgaaggcca
     1381 ttctgtagaa caacgtgcca agaacgtcgg tggcgaaaaa gggcccgcct tccgagcctt
     1441 ccatcgcaaa gacaagctcg aaggtattca cggaggcgat gaaattcaga acgatgagcg
     1501 tgatgagcgt cggccacagc aagggaatca caatcgtcgc gaccacccgc agctcgctcg
     1561 ccccgtcgag gcgcgcggct tcaagaagat ctgacgggat acccaggatt gccgcgagga
     1621 agatcaatac cgggaatccc atgttcgccc aggcacttac tgcgacaatc gatggcaagg
     1681 caaaatcggg atcaccaagc cagggtcggg caagggaatc gagcccaata ctccgcagca
     1741 cggcattgac cgccccaaac tgggggttga gcaacaggtt ccagagaaaa cccacgacca
     1801 ccaccgatag cgtgttgggc atgaagtaga gcgcttggta gaaccgcgaa cccgggattt
     1861 tccggctgag gatcagcgcg aaccccaagc cgacaaaggt ggacaggacg gtgctcaaaa
     1921 agaacacctg tgcggtgttg ctcaacgcat ttgaaaactg gcggttgatc ggtgcgcgat
     1981 tgaaaaggtt tgtgtaattg cccaagccga cgaagtcatc aaggggacca agcccgctcc
     2041 agttgtaaaa gctgacccgg atcgtattgg caaacggcca gatgatgaac gcgagataca
     2101 tcgccaggcc aggcacgagc atcaggaggt aggtgtgctg cttgatggca actgcacgcc
     2161 cacgcctgcc ggggagtgac gtatcagcgt cggcggcaac cgacgggcga cgcaggaacc
     2221 ggaatacgcg catggagcgt ccctcttcgt agagcgaggg tggtagccgg ggccgaatcg
     2281 acggccccgg ccggtccact agccttggcc gttctggaac ggctcatacc attgagccag
     2341 gccctcctgg agttgctggg caacctcctc cggagtcacc tggtcgctca acaacccctg
     2401 cataagcgga tgcagcagcg tggagatacc gggatcgccc tggtcgaacg gagaccggat
     2461 gcggtacagg ttctggatcg cgtgctccgc gaactcgatc tgccgctgca agtatttgtt
     2521 ctcctccgta gccgtcacgc ccggaatcga tgacatctcc tgcgtgatgt cggagaaggt
     2581 ctggccgtag tcaacggtcg cggagtactt cacgagctca agtgctgcct cttgcacctc
     2641 agaggccgca ttgacggcgt agcctccgtc cacaaagaca tagctcgaag gctcctcgcc
     2701 tgcctcatcc ggcgggctca ggaagaagtc gaactccagg tccggcgcct gctcgatgta
     2761 ggtgttggcc gcccagcctc cgtacaggat catggccgcc tgtcctgact ggaacgcggc
     2821 atccatatct ggaacgttgt tcccgacgta gccctcctgg aagaagggtt gcagatcctt
     2881 gatccgggag agcaggctga cgaatttctc gtccgtgaac tgcttttctc ccgcgatcag
     2941 ctggccggcg aactcgtccc caagataggt tgagccgatg cagtcgacat agcccgagag
     3001 cacccagccc tcccgcgcgg ggacgaacaa cggggtgaca ccggcatcct tcagggtttg
     3061 gaaagttgac atgaggtcgc tccacgtctc cggtggcgtg agcccatgct cctcgaagat
     3121 ctgggtgttg tagtagaaga cccacacctg gctggcgaat ggaaccgcca ggacctcgtc
     3181 cccgaagctg acctgcccga gcgtcccttc cggaatctcc gcgagacctg gcaccttgtc
     3241 cgtcagcggc acgaacatgt tcgcctcggc atacggccgg aagtagccct cgccggcgcg
     3301 agtcgtaatg atgtctggcc cctgcccgcc ctgcattgcg gtggagactt ttgtcccgaa
     3361 ctccgtagcg tcgctgacct caacttcgac ctcaagacct ggccatgtct cttgcaggtc
     3421 ttccgccgct gcctgccaca cgaggacatc atcctcgcgc catgaccaga tggtgacggt
     3481 tacgggctcg cgatcggcgg caggggtcgc ctggatcgcg gccgcggcac gaccaccagt
     3541 agccagggaa acggcagcag acgcagatag cccaagaccc acggccctgg taatgaactg
     3601 acgtcgagag agctcattat tgtgcagctg tgacagaagt ttctgtatgt cgctcattgc
     3661 tggctccttg taagtgattg gtatttcgcg aaggcagaaa tcggccactg actccggcgc
     3721 tgctgcggcc ctatcccgcg aaaggctgaa cgaactccct tatgtcccgg catcacctcc
     3781 cgtcctgata tatagatagc ttccaggcga aacgacaacg caatgcatgg tgtggggtag
     3841 ccgtaccgtg aaacatccgc caaccacgtc gatgccgatg taccaggcaa ccttgatccg
     3901 ggagagcact cttcatggcc aatgccgtcg aagcgcacgc actgcgcctt tgctcgatat
     3961 cgttatcctc ccggaccaga ttcctggcat agtcattcag ggacatattg ctgtcaagcc
     4021 ccggaggggt ccacgcgatc caatcccgtt caccagccgc attgggtagc aatttgccgg
     4081 caccctaacc ccccaccgga aatcgggaaa gggaagaggg aggagcgctt tctggctcct
     4141 ccctcttcgc gtgagctcct gttggtctac ccactctccc ttagctcaat ggcggcaggt
     4201 agttcttcgg gtcctgcgtg accccgtcct gtcgcaccat gaagtgcatg tgaggtccgg
     4261 tcgaaagacc cgtgctaccc accgggccaa tcacgtcacc ttgggcgacc gtttgcccta
     4321 ctgcgacata tggctgggaa gccatgtggc catacaccga gtgaagcccg gaaccatgat
     4381 cgatctcgac gtagtacccg agaccgcaat cgcaccaccc cgaagccacc acggtgccac
     4441 cctccgctgc ctggatcggc gtccctgaag gagccgccag atcgacgccg tcatgcaccg
     4501 agcatccaaa ggtgggattg taggtgtaga aaccaagcga gctgcagccg tagtcttggg
     4561 tgaaggtgaa ccggctcagc ggcaggatga agcccgtggc cgcatcttcg gtagctgttt
     4621 caccttcggt atcggtttcc gcgggtgcgg tgacgtcttc aacctgtgcc ggtgcctcag
     4681 ttggcaccag caaatcaccc cgtgcccatc cactctggcc accgtcagta acccgatacc
     4741 atgccacgcc ttcggcatca gccaaaggac cctcggtcac agttaccact gatccctcag
     4801 tgaggaatcc cgcccgctcg gactcctgcg tcggctcctc ccggatgttg atcccgacat
     4861 cactgccggc atctaccatg gcgaagtcgc cctcggtcaa ggttccgacc gtatcaggcg
     4921 cgtcgatcgc ggcggtttcg accgacgccg cctcagtgtc cgaagcccgc gcttcactcg
     4981 ccagcatggg aggatttgcg ggaatcagga gcgaaccatc gacgtatcca acctggcgat
     5041 cccagcgaac ctgataccag ccattctcag ggccgcccag gatttcgacc aacgcctgat
     5101 tgggcgcgaa gccagactga tccgcattcg cgctggcccc ggcacgcagg ttcacaccgc
     5161 tgccgtcact ggtgcggatt tccgcccact caccggccaa aaagacgccg tccgtgggat
     5221 ctgccgatgg agggtcaagc gtcgtgggaa tcgtgaccgt aggagcaggc tgtgttccag
     5281 ccgtgcctgg atcgatcagg tagaagccgg atacccagcc ttccagcccg tcgacagcca
     5341 caggccacca ccgggtgccc tgctcgtcgt agacggtgtc cagttcatcg atccgcagct
     5401 ctacgatggt gccttccgac agggaagcaa ccttggcact gttgctgtcc ggctcgaccc
     5461 gcagcacgag cccgtcacca tcggcggaga tctccgcagt catcgagccg gtgtactcat
     5521 acggcgtcct gctcggtgct gaggtcgtag acgttgtgcc tgcggtgcca tccgaaggca
     5581 caggggtatc gggatccgtc aggtagaagc caacgatcca tccatccaga ccattaaagg
     5641 aaaccggcca ccagcgagtg accccatcgg ggtcgtagac cgtgtcgacc gcgtccacgc
     5701 gaagatccac gatggtgcca tcgggcaccg ttgccactac ctccgcgtcg tgttcggccg
     5761 cggcgcggag attggccccg tcgccatcag ccacgacgcg tgcggtcagg ccgatcagcc
     5821 catcggcgcc gctgatatca aactggtcgt ccgtggagac cacggctgtc ggcgtgacga
     5881 catcgtcgcc cgacgccagt gttcccgaca ccgcgggaaa catggcacta accgccaata
     5941 ttcccgcgag cgcgaacatg cgaagacgcc ccttgaagga cgtatatgtc gaagcggtgg
     6001 tcaccgcgcc ttccctcctg aaatgaccga aacgaaagcg ggaccggggc cgatgtacac
     6061 agacgagaga gaccgattca tgccgtggga atacctcgat gtgccggcca gagacctctc
     6121 cgaccaacac catcgatcca tccggttccc cacgtgctcg cgctctccgt ttgtggacat
     6181 catgtgaccc acagttcccc actcgcgctg tagttccgta ccagcagatt ccactgctcc
     6241 gggcatgatc gatgggtcgg tgctgccgga tgtaaagatc gtccggacat gttcagcgcc
     6301 gactgcctcc cgtcatgccg aaggagggaa gatcattgcc tgaatggacg ataacaccca
     6361 cgccagcaat gatcaatgtg tacgaacgct ttgttcgtcc ggaaatgtca ttactggacg
     6421 ttcacacgtt acggacacgt ctccaaacac cgcaagaggg cccgaacgga gcaccagacg
     6481 aggagacgga accagcctga ctctcaatat gatcaatacc ccggctgggc agcgggcacg
     6541 aggggatccc tgacgaaagg gtttgtgagc ctaagcgacc cagtcgaatc ggcagtcatc
     6601 gtgtgggcgc tccccaccgc tggaagcaat cctgctatac ttcggcgctg ttgatgtgac
     6661 cgtcatggcg gtcattttgc tgtgctccgg gcaggcttgc acccggagac acctattggc
     6721 caagcacggt gcgcagctgc ggaagcgtca gcgcaaccat gaacgccggc acccggcaat
     6781 tccggcatcc cgtgcagcca cgccatcgag aaaagagaag ggacgcgaca agcgtgaaac
     6841 tcactactga acggaagccc ggaagccttg tcgaactgaa catcaccgcc gaggacaagg
     6901 agttccagga agcgctcgac aaggccattt cccggcaggc ccgtaatctt caggttcccg
     6961 gtttccgcaa gggcaaggca ccgcgtcgaa tggtcgagag ctattacggc cgggaagcct
     7021 tccttcgtga tgccgccgac gaggttatgg accgccttta ccgccaggcc ctcgagcagg
     7081 aagatatccg cccggtgggc gagccggatg tcgagatcgt tgacctggaa ccggtcaatt
     7141 tcgtcgtaac cgtgccggtc tacccgagct tcgagctcgg cgactacacc gccgtccgcc
     7201 aggagccgat cgacgccacg gtgactgacg aggacgtgga tgaagtcctg aagcgcctgc
     7261 agacccagca gtcgtcgtgg gccgagttaa gcgaggagcg tacacctcgc gaaggcgacc
     7321 agatcaccat cgattactcg gtcaaggaag gcgaccagga cttccaggag ccggtgaccg
     7381 atgccgtctg ggtgcttggt gagaccaacc tgttgcagca gctccgcgac aagatcgagg
     7441 agatgcatgt cggtgacacc gaagaattcg atctcgtatt cgaggaagat gatgagactg
     7501 ccgacccgca gattcgcggc aaggagctca agtactctgt caccctcaag gggctgaaag
     7561 agcgccagct cagcgagatc gacgacgaat tcgccaagac ggtcgccgat gcggagtcag
     7621 tcgaggattt gaagcagcag attcgcgagg acatccatca gggcaagacc aatgacgccc
     7681 gcaatgaggt cctcaatgcg attgtcgacc agatagccga gcagacagat ctcgacctcc
     7741 ccgaggcaat gatcgacgag gaggtcgagc accagctcgg ccaccgcaag caggagatgc
     7801 agcagcaagg catgcagtgg gagcaggtcc tgcgaatgtc gaacacgacc gaagaggctg
     7861 tgaaggctga catgcgtccc gacgccaccc gccgcctgcg caactcgatg ctcctccagg
     7921 agattgcaaa gcgcgagaat atcggggtaa gcgagcagga tctggatgcg gagatcgacc
     7981 gcgttgccgg tgcggacctg aacccggacg tggatgacga ggaagccaag gctcgtgccg
     8041 cgcggctgcg cgaggtctac aacagcgatt acttccggaa tgtcctccgc aacgacatgt
     8101 tcgagaagaa gctgaccgac cgcatcatcg agatcgccac cgagggcagg ggcgccgtcc
     8161 tcaatggctg gacggcacca gaccctgctt caacctccgg tacggacgag acggctgagc
     8221 cagcgagcga gacgccttca aatgaagcgg cccccgacga ggtcgtgacg accgctgaag
     8281 cgtccacggc gaacgaaatc agcgaccagt aatcgccggt tccaacgcac tatatgatcc
     8341 tgttatatga tcctgagaag cacgagcatc tgccgcatgg cgggcgctcg tgcttccatt
     8401 tccggagtcc ggagcatttc ctgtgagcgc gaaccgcacg ccggatgacc tcgccagata
     8461 catgtggctc ctctcccccg aggggcagga tcttctcgac cgcatccgcg agatgacggc
     8521 caaaggcacg agcgacctca ggatcgcgga gcatctccgc tcctcatatc ccgctgatcg
     8581 ggtggccctc gcgatgaccc agcacgacct tcgcctgaaa gcgcgggaca agttcgcaca
     8641 ggctgatcgc ctcttcttca ctcgggaagg gctcgagcag gcaacatcgg accggatcgc
     8701 ggcccaccgt gcagatcgct tcgcctccac cggtcgggtc atcgatctct gctctggcgt
     8761 cggtggtgac ctcatggcgc ttgcaggctt gccaggcatc catcacctcg cggcagttga
     8821 cctcgatccc gtgcatctgc tgcttgcagg agccaatgtt gccgccgtcg cgcccgaatc
     8881 tgagcccagg ttgatccacg ccgatgttcg ccaggtggat ctttctgcct tcgacggcgt
     8941 cttcatcgat cccgccagga ggagcaccag cggccggttc ggcggcatgg gcagtgaacc
     9001 gccgctcgag tgggccttgt gcttaacgga tcacgtaccc gctgtcggga tcaagaccgc
     9061 gcccggtatc cctcacgatc tcgtgccaga gggctgggag atcgagacta tctcgcttgg
     9121 gcctgatatc aaggaggccg tactctggtc accggccctc gcatctgagc cagcaaccgc
     9181 cacggtgatc gcaggagacg ccgtgcacca gctgcgttac atccttggat ctgtcgttcc
     9241 gatgcgacga cctgaagcgg gccagtggct actggacccc aaccctgccg tgacccgcgc
     9301 cggactggtc gaagatctcg cccgactgct ccatgctgcc aggatcgata aggagatcgc
     9361 cttccttatt agcgactcgc cggttgagac tccctttgcg cggtcgctgc cggtcatcga
     9421 cgcattgccg tggcacgaaa agcgaatccg tgaacgggtc cgggaactcg atggtggtcc
     9481 cgtcgatatc cgccggcggg ggcttgccgg cgatgtggag acgattacca ggcggttgcg
     9541 aggtaagggc agccgccgtc tcgtcatcgc aatgactcgc gtaaaggatg agccatgggc
     9601 cgtgatctgc gatatcgcat gaaatgcgtc cgttacggta tggtgtccgt gcaccgctaa
     9661 cgtggcgttc tcgacaatgg gcggtatcat attaatacgg ccccgttccg caccacgccg
     9721 tcgctcaatg cttgagtggg acactgcaac agagagacca ggaagagagt cgacgcctcc
     9781 atggaatacc cggacatcca caatctcgtg ccaatggtgg tcgagagcac gaatcgcggc
     9841 gagcgcgcat tcgacatcta ttcgcgtctc ttgaaggacc gcatcatctt tctcggcacc
     9901 cctgtcgaga gccagatggc gaatctcatc atcgcccagc tgctgttcct cgatcacgac
     9961 gatcctgagg cagacatcag catctacatc aactcccccg gcggcgaggt ctattccggt
    10021 ctcgcgatct acgacaccat gcagatgatc cgcccggaca tccagaccta ttgcgttggt
    10081 atgggtgcca gcatggctgc cgtcctcctt gccggtggca cagcaggcaa gcgcttcgcg
    10141 ctgccgaaca gccgcatcat gatccaccag ggatcggggg gtttccgggg caatatcccg
    10201 gatcttgagg ttcaggccaa ggagaccttt tccgttatca acaaactgac cgagattctc
    10261 gcaaagcact ccggccagcc gtttgacaag gtcaaggccg acacccagcg cgactactac
    10321 atgaccggtg aggacgcgat gaactacggc ctggtcgaca aggtcctgac cccggccgcg
    10381 atcgatctct cgaagatcgg aaagaacggc tccagcgagg actagtcgcg tcgaatcttt
    10441 gatcctgtag cacccggtgc ttctccatcg gatacctccc ttctccgggt gcaacaggat
    10501 tcgtgaccac agatgaggtg gatacgtgca ttaccggtgt tcattctgta acaagggcca
    10561 ggaagaggtt cgccgcctga tcgccgggcc gaaccaggtt tatatctgcg acgagtgcgt
    10621 gcagctctgt cgcgagatca tcgaggaaga agagccgtcg accccgaaag tcgagccggt
    10681 ggcgaccaat atccccacgc ccaaggccct gtatgagcac ttgaataact acatcatcgg
    10741 tcaggaccgt gcgaagaaga tcctctccgt cgcggtctac aatcattaca agcgcatcaa
    10801 tgcgggcgtc caggaggccg atggcgtcga gctgcagaag agcaatgtgc tcctcgttgg
    10861 tccaacgggt tccgggaaga cctacctggc ccagaccctc gcgcgcatcc tcgatgttcc
    10921 cttctcgatc gccgatgcca cggccctgac tgaggccggt tatgtcggtg aggatgtgga
    10981 gaacatcctg cttcgcctga tccagaacgc cggtgacgtt gcccgcgccc agcggggcat
    11041 catctacatc gacgagatcg acaagatcgc ccgcaagtcg gataacccga gcatcacccg
    11101 cgacgtctcc ggcgaaggtg tgcagcaggc gctccttaag atcatcgagg gcaccgttgc
    11161 caatgtgccc ccgcagagcg gccgcaagca cccgcaccag gaatacatcc agatcgatac
    11221 gaagaacatc ctcttcatct gcggtggtgc cttcgagggg ctggaggaga tcgtctccaa
    11281 gcgcatcggc aagcagagcc tgatgggctt cggtcacgag gccgaagaag ccgccgcaaa
    11341 aatcgacaac ccactgagcc agatgactca tgacgacctg atgaagtacg ggctcattcc
    11401 ggaatttgtc ggccgtctcc cggtctcggt ctcgctggat cacctcaccc gcaaggagct
    11461 gatgcagatc ctcgtcgagc cgaagaacgc cgtggtcaag cagtaccaga agttcttcgc
    11521 cctggacgat gtcgagctca tgttcaccga ggatgcgctc gaagccgccg ctgcccaggc
    11581 ggaagcccgc aagaccggcg cgcgcggcct tcggaccacc atcgaggagg tcctgctcga
    11641 ggtgatgtac gagattccgt cgcttgagaa cgtccgcaag tgcattgtcg acgcgaacgt
    11701 gatcaccaac cgctctcgac cgctgctgct ctcggtcaac gaacaaccga ttgagtacgc
    11761 gcagacggcg tagaggcgat caccacatca gcaaccagaa ggccggccag gcggtcggcc
    11821 ttctgtcatg cctggggcgg gtgctgccgc cccctcagct gatcccgtat cgcctcgcga
    11881 accgccagac gcaaccatcg actgttcaac ccatccgtcg agtcgatgaa ctggtcgatc
    11941 cctccaacgc cgagcggcag cgcctccagg actgttccgc gcaatgtctc gcgcaaggca
    12001 tctgccactc gggcggcgtc aatcactgtg aacggccgag aatggaacgg gcgcggcgcg
    12061 ggatcgatct ccgtcgtcaa ccccagggca ttgtgctccc ggaccagggc ggacatcgcc
    12121 gcgacgatcc ctgcctcccg cttacgccag tcccccgcct gccggaccac atccagatgt
    12181 ggccgtaaca tgttggagac gggaagccgt gtgaaagcgg tgccaagcca cttcgaatac
    12241 ggcgcgtagt gccgcgtctg cgacagtgcc agccgcatga tgtcctcgac caatcgcccg
    12301 ccaatcacga acgacccggt ctcgtcacca acctccccag tccgcccgat aaatggctcg
    12361 atctgcgaaa tgcgcatcca cagcgccgcc atccggtacc tccacacgtc atccggataa
    12421 aatgacaacg ctgatctcaa cctgcttaac tccccggtat catcccggaa caaatcaccc
    12481 gccgtgagtt ccagcaaccg ctggtcagtg accgagagtc agaacaccgg gtcagcctcg
    12541 tcgcccggcg cgtaatcgac tcgcgcgtgt aacgcgtcca ttgtggtcac gtcgaccaga
    12601 tgccgcgttg gcgcgtcccg ggcagggtcg gcgggaacga cagcgcccgg ctcatccgaa
    12661 gcatcctgga acaacgccgg gaagccgcga aaaactggcg gcatggcgtc tgcaatcgcc
    12721 tcccgggtgc gatcgccaaa caccagctca tcctctttgt ccagcaccag cacgcaccgg
    12781 ggaccccagt cgtgatccat cgatcgtgca gtgtcatacc ccaacgcctc ggagccgact
    12841 ccgaacaaac cggcggcata ccgcagcccc ggcattgtcc ttgtcagcga cggctggatg
    12901 acctcatcaa agagcgcacg cgaaagctcc attcccggta cgaaccgggg tttgcctctt
    12961 gctggtgcct tatcgtcgcg cttcatgaaa ctctttaggc tctttctgtt tcgttgcgca
    13021 gtaaattggt gccaggcccg atcataccag ccgcccgaca aaggatccgc ggcgtggcgt
    13081 ttgcgattat tcaggaacga tcggctggtt gcaggagacc caacacgatg accaacgaac
    13141 ccgttcgcga tgtccagatt cgcgttaccg gccatgtgca gggcgtcggt ttccgcgtgg
    13201 gcgcccgcca ccgggcgcga gaactcggag tccacctcca tgctgagaac cagccagatg
    13261 gctcagtcct gctggaggcg aagggcaacg ccgacgccgt cgagcggctc gtggtctggg
    13321 cgaagcaagg cccccgcaat gcgcgcgtcg atcatgtcga agtcacgaac cgcgacgccg
    13381 gggcggatgg ctttgcctga tctggcacta ccactgcgat gacccttccg ttacggtaag
    13441 gccaggttcc gatcggaacc cggcgttcgc ctatcccgaa cggagagccc aaatgaatcc
    13501 tgatcctctc gcccgccgtc cctttctcga ttacgacgtt ccgcctatcg ccgtcggttg
    13561 cgcaccgctt ggcgacatgc ccgatacgtt tgattactcc gttccgcctg aaatcgcaca
    13621 agcgacggta cgcgccgcgc ttgccagtcc actgaattgg ctggataccg ctgcatccta
    13681 cggagacggt gaaagcgaac gcaggatcgg gaaggtgctc cgggagttgg gaggcttgcc
    13741 ggacggtgcc tttctcgata caaaagtggg tgccagcccc gacggcgact acagcgctga
    13801 aaccgtgaaa cgccgcttcg cgcgaagcca ggaactgctc ggcatggagc ggctagagat
    13861 ggtcttcctc catgacccgg aaaaggtctc ctatgaggag atcgcggcgc cggacggtcc
    13921 cctggcagtg cttcagggct accgggagga ggggctgatc cggcacctcg gtgttgcggg
    13981 cggtccgatc gacctcatga tccgctacgt cgagaccggc atcttcgacg cggtcatcac
    14041 ccacaatcgt tacacgttac tcgatcagtc ggctgatccc ctactcgacc tctgccacgt
    14101 caggaatatt ccagttctca acgccgcccc gtacggaagc ggcatgctcg ccaaggggcc
    14161 atccctctac ccgaggtacg cctattcacg ggcgacagac gacatgatcg accgcgggcg
    14221 gcagctggaa gccatctgtt ctcgtcacaa tgttcctctc gccgccgtgg cacttcagtt
    14281 ctcgatgcgt gatccccgcg ttaccgcgac gatcgtcggg ctttcccggc cggagcgtat
    14341 cgagcagacg atcgatctcg ccacagggga aatcccggat acctgctggg atgaagtcct
    14401 ggaagtagcc accggctcgc gcagtacggg gtaaagagaa tacccggagg caacggctgc
    14461 tgaccgtccg ttctcagacc atgcacgatg tccactaggg accgagacgc acgttgaagc
    14521 gctccattag ttcgggaagg cgctcaaggt gcatcgtcag cccataccgt ccggcaatcc
    14581 gcgccaaccc acccatatcc ggtgggcctg ccgccgagag caaatcggcc acttcgacaa
    14641 aatactgctc gaagccggga ggcgacacga tctcgacaaa cctggctggc acactggttg
    14701 cgttccagaa cgcatgggga agaccccgtg gcttgacaac ataggcgcct gctcccgcgg
    14761 tgaagacatc ctccccgatc aacacgcgga tctccccttc gagcaagatg gacacttcgt
    14821 cttcgttcgc atgcgtgtgc ggtggagagc cgagttgccc tggcagcagg acgtgctcaa
    14881 caacggcata ggcgcctgct gtttgcgcac cgggtacctt gaagatagct tccagccccc
    14941 caagcgatac agacctgccg ctctccggtg gcacaaagag cccttgattc tcgagaacca
    15001 tggtctgctc ccgcattcac gttcaacaaa tgcctgctgt gtcccggtcg ccggagtata
    15061 gcatctaacc ctcgttttct cggtagtata gggaaaatcc ggatcgttgc ccttgcgcga
    15121 atcgggtgcg agcggtatag tcacagttat gaatacgatt gtgcgtgaca ccaccggcac
    15181 ccatatgcag acgatcagct cgatgactac cattgagatg attatttgct ggcctgccgg
    15241 gccgggtgtt tgctgacgca cgacgtcgag tcacaaacat gaaaccggaa cggcaggcgg
    15301 gagcacacac attgctcccg cctctttgtg tgcatgggca ccaacgacag gacaggctca
    15361 cggcgagcaa cgatgcttct ccgggaaccg ggacaccgag tgggacaagg gtccgtctca
    15421 acgtccaaac gtgcagcggc cacgggccgg cacatcagtg cctggtgagg agattgatca
    15481 atgcgagtcc taaaattcgg aggaacatcc gttgggaatg cccgtcgcgt ccgcgaatct
    15541 gcgcgaatcg cgctgaatac tcccggcacc aacgttattg ttgtttccgc gagtggaggc
    15601 gtcaccaatc tgctcattga tgccgcccgt gccgcggccc gtcatgatcg cgatgccgtc
    15661 gcaagcgctg tcgcggagat tcacgcccgc cacgagaacg tgctctctgg tatcggggag
    15721 gaccagctca gggccaccgc ctctggtgcc gtcaaccaga tccatggctc cctcacttca
    15781 accctcgacg aggtgttcac ccaaggtcag ctttcgccga agctctctga caaggtcgtc
    15841 tcccatggcg agaaggcaat ggccctgatg atggcggcca tggtccgcga catgggaaca
    15901 ccggccgaac ccgtattcac cgataccgtg atcgccacag atggccgtca ttccgccgcc
    15961 cggccggacc gtgagcagac gagagccgct gccgccagca agatcctccc cttgctccag
    16021 tcgggaacca cagctgtggc aacaggcttc atcggcttcg gaccggatgg cgcgacaacg
    16081 acccttgggc gaagcggctc cgactacagt gccacgctgc tcggcgccgc gctcgatgct
    16141 gaagaggtcc agatctggac agatgttccg ggcgttctct cggccgatcc gcggaaggta
    16201 cctgcggcac gcgtcatctc cgagatcagt tatgacgagg cgcaggagct cgcccatttt
    16261 ggggcaaagg tgctccaccc gcgaacgatc cgcccggctg tcgcccagaa catccccgtc
    16321 cgcatcctct cgaccttcca gccaaccaac tccggcacgg ttgtcacctc ccgggccctg
    16381 gcccagggta tcaaggccac caccgcgctg aaaggcctta cgctgctgac gctcgacgtc
    16441 ccggaactcg aggatcttgc gccggccgcg tccgtcatct ttcgtgtcct ccatgagcaa
    16501 cgggtggaga tcctcaccgt ctcccaatct tcctcgcggc ggcgcatgag cttcattgtc
    16561 gataccgtcg ggggcggctg cgagatgctc actgcccaga tcactgctgc tctggacgat
    16621 atcgaggccg atatctcctg cagcgataac gttgccgttg tggccgcggt cggtgatggt
    16681 gcagcaaact tgccatcatc gctcgcgcgg atgctcgcag tactccaccg ggcaggtatc
    16741 gaggttcttg caacgaacca gcagaattcg aatgcggcaa tggtcgtcgt tgtgccggag
    16801 gacgtctcgg atcacgcggt ccaggtggtg cacgatgcct ttatcggccc atcacggcgt
    16861 agcaaccgct cggcatcacg tcgccgcagt gatctccttg gcgaatcgct acgggtcggt
    16921 taatctgaag aatcaacgaa agggtcaggt atcaatgacc gatggacgaa ttcccgttgc
    16981 gattctcggc gccacaggaa atgtcgggca gcgttttgtc cagttgttgc aagatcatcc
    17041 atggtttcgt gttgcggaac tggttgcttc ggagcgctcc gcgggcaaga gctaccgtga
    17101 tgccactgac tggcgcctcg gggcatgcat gccggaaggt gtcacggagc tgacggtgca
    17161 ggattacaac gccgatctgc agagccccct ggccttctcc gggctgccgg gtgaggtcgc
    17221 cggtgatatc gagcagcgtt tggcgaagaa tgggcttgcc gtcctttcga atacctcaac
    17281 gcaccgtatg gagccagacg tgccgctcct gatcccggag gtcaacgccg accacgttgc
    17341 tgcaatcgag gtccaaaagg ccaaccgggg ctggtccggt ttcatcgcca ccaacccaaa
    17401 ctgctccacg atccacctcg tgcttgcact gaagccactc catgatgcat tcgggctcga
    17461 cagcgtcttg gtgacaaccc tccaggccgt ctcgggcgcg ggctaccccg gtgtaccgtc
    17521 gctcgacatg atcgacaaca tcgttcccta catcggcggt gaggaagaga agatcgagac
    17581 cgaagcaatg aagctccttg gtggttacga acccgatcag ggttttatcc ccgactcgtt
    17641 cacgctttcc gcccactgca atcgcgttcc cgtgcgcgac ggtcacacag agtgcgcctc
    17701 agtcgggctc acgcagccgt cgacaccggc cgaagcgatc gaggtgttcg agtccttccg
    17761 gggtgtgccg caggagcttg ggctgccaag cgcgccggag cggccggtta tcgtccgtta
    17821 cgaggacaac cggccccagc cggccctcga ccgtgatgac tcgaacggca tggcgtcggt
    17881 cgtcggccgc gtgcgcgagt gtccagtact cgggctgaaa tttgtcgtcc ttggccacaa
    17941 caccgtccgt ggggccgcgg gcgcatcgat cctcaacgca gagttgttct gcgaaaaagg
    18001 cattcttcca atttagtgtg aaccagacca gaagaccccg gcccgtgggg ccggggtctt
    18061 gttgttgctt acgcatctcc gccaggcacc agcagaagtt cctacctgcc ccagaacgcg
    18121 aaaacgccgg acagaggggg tgtccggcgt cttcctgtgg gtggaggaag ggggaataga
    18181 ttgtcgtccc tgtcgtactc cggcaacgga gtctctcctc gacaggacat agaccgcgcg
    18241 catcaggatc cagcaccaca caggatcatg gtgatgtcac gtccggatat ctgggaggga
    18301 cgctgctggc accacggtgg ctgggtccgt gttcggtgcc gggtcgcctc cccgtctccg
    18361 gaagggtcac ccatagtctg cagggtttgc agaggggggt catcagtaga aatacgtatt
    18421 tcggctggga ccaattacgt aggttctccg acatggagat cggagccggt gtctcgtgca
    18481 cacagagaac gacttctcct gtgattccat gccagtcatc agaacgagtc gtccctggca
    18541 cccctagcat atcgtcacgg ctatggggtc gatgagcaaa aaaggacatc cggcaacgta
    18601 agggcactga ggccgacgaa gcaagtaggc gctcactctt ttctcgctta gccatgcatg
    18661 gcgcgggctc ggatcccgcc tacgatgagc gctcttgacc gtccagctgg tgcaccaact
    18721 tcccgagctg gtcgcttgtc agctggttgg ccatgtacat cccgacgatg atggcaaaga
    18781 gacggtccgt ggtggcctgg gcatcagctg gccagtcctt gcgttgctca tagggcggca
    18841 ggagcgcgga agcaagttcg tcgatctcgt gtctgacgtt catgaaggtc cttgaagatg
    18901 tgcgaaaggc gagcatggtg gttcaccaca atgccgcact tctcgtgcgc cagtagttcg
    18961 ttcggtaatc gcctgatggt tgcagtgacc gtgccaggaa taccgatcct gtttactggc
    19021 ggtcgagtca ggagtgctga cctgcgctgg gtaccggcgc aaggagagat gttactgctt
    19081 cgaggagtgc atcgatattg aagggtttgg agatcacctg gacacccaat gccttcagct
    19141 gtggtcgaat cggcgcgacc tgacgcagtg cgccggtgca gaggatgacc ggtatccccc
    19201 cgatgcgaga gtccctcctg agtgcctcaa ctgctctcaa gggaacctga tccccaccct
    19261 gtataaagtc gagaataaca aggtcaggac gcatcatcgc aatctggtca acatcatggt
    19321 tcgcgaaggt cccggtagac acatgatact gctcaccctc gagcacctcc tggtagaggg
    19381 agagaatatc cggagaatca tcaagggcaa ggatatgggg ttgataatcg gtgccgtaca
    19441 cgacgcttgc tctcgcttca aatgaggcgg aacggtcctg agtcactcag gactcctgtt
    19501 gcacaactta tacatgaagt ataggttcat ggttcggttc acgcaaggcg gatagccatt
    19561 gggaagagac agcgaggccc gcgcagggaa agtccaggcg catattggat tgatccatca
    19621 gaagtgggcg ggccacacca tcactacgtc cgccgaagcg ccatgaagta acccgggctt
    19681 aacaattcca ctatctcgat gcgctaagat cagaaacatc gtggttacac cagatacgtc
    19741 cagttgtcgg acccgatcca ggcaccgccc ccatgcattc cgcgatcgca tgcgatgcgt
    19801 gcgcggcttc acccactgcc gcacccaatg agccgtgatt cgggtgcgtc ccagccatac
    19861 ggcagcgagc cgtaacgcca tggaccgtgc gccataaaaa agaagggaag tcggataccg
    19921 atgacactcc agcaccaggg gacccagtgg tcgacccttc cctatgatga atggcaagca
    19981 acccaggcaa ccctccatcg atggatgcag atcgtgggca aggtgacgct cgagggcaca
    20041 ccgttcctcc acgagtggtg gaacgtcggg cccacggtga cgtccccggg gcatgaccac
    20101 ccccttgatc ccgtatggcc aggaggcgtt caccatcgcc tttgacttca tcgatcacac
    20161 gctctccatc gatgtaaccg atgggcggac cgggagacta ccgctgcgtc c
//
LOCUS       DASYWM010000221        27322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_65462, whole genome shotgun sequence.
ACCESSION   DASYWM010000221 DASYWM010000000
VERSION     DASYWM010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 27322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27322
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65462"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VGR29_08930"
     CDS             <1..364
                     /locus_tag="VGR29_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2073749.1"
                     /translation="VLTFLEKHFDWEWHREASDIFNSLYNGDGRNVGMLIVTQGDEIL
                     GYAQHRGERFGPFGVRPDLRSKGIGRVLLTHTLRQMRASGFHVAWFLWTGDNAARLYA
                     RYGFHEVRRFAVLRKTLT"
     gene            462..1190
                     /locus_tag="VGR29_08935"
     CDS             462..1190
                     /locus_tag="VGR29_08935"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV2073750.1"
                     /translation="MASNSDNCIMVVGGHAADAEVMGGASVLKHVDAGWRAVNLHLTG
                     GEKGHKSLSPEEYLEIKKGEAAEAAKRLGAESIFLSYKDGELPVDAEVQMAIADVIRE
                     YKPTIILTHWKGSIHRDHTNTYENVLASLFFAGLRTFERKYPPHWVSHIYFAENWEDE
                     EGFVPEVYLDTTDVFDHYIHAIHAYSLFRGEAAKFPYEQWYRGASMMRGAEVGYEQAV
                     ALMNHRTYYGSRKKAGLLGPEDKR"
     gene            1187..2344
                     /locus_tag="VGR29_08940"
     CDS             1187..2344
                     /locus_tag="VGR29_08940"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEV2073751.1"
                     /translation="MSAHVTTGLEVLLDEGHPLVEGRRIGLVTNHTAIDRDLRSVIDL
                     LHTDSRFELAHLYGPEHGVRGDVQAGVHVGRAIDGETGLPVDSLYGETRKPTPEMLDG
                     VDILVFDIQDAGARFYTYISTMVLSMEGASEAGIPYVVLDRPNPITGVHVEGPMLDPQ
                     FESFVGIHPIPTRHGLTVGELARLVAADRGWPEPTVIRMQGWQRDMWYDETGLPFVQA
                     SPNLPTMDSLTVYTGTCIFEATTLSEGRGTTRPFEYIGAPWINATELVAELRWRDLAG
                     VGFRPASFVPMFSKHEGETCSGVQLHILDREAFRPVEVGVHLVHAIKHLNGSQFAWRP
                     GSATGMSANLLYGSEVLHAMIDDDASPEEIMATWDDGQDAFREKAGAAYLY"
     gene            2686..3255
                     /locus_tag="VGR29_08945"
     CDS             2686..3255
                     /locus_tag="VGR29_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073752.1"
                     /translation="MNDPAPPQMSDDAVQRGSGKTWDEWRAILDAWGATNKPHDEIAQ
                     YLVEDHGIDGWWAQGVTVGYERLIGRRAVGQRADGTFSTSASRTVLAGIEEHFVAWTD
                     DTARDAWLTPGILSLRTAQEHRSARFDDNEYGGIVALYFSSKGEGKSSVNIQIEKLPS
                     PDAIPERKSIWKARLDDLATYLENARSTD"
     gene            complement(3276..4007)
                     /locus_tag="VGR29_08950"
     CDS             complement(3276..4007)
                     /locus_tag="VGR29_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2073753.1"
                     /translation="MPEGWLAVDGVELWVADHGSGYPVLLCPGGPGCGDYLAPVSGLI
                     DDMVRVVWWEPRGCGRSGSTGPYDLATTLADMDAIREAMGVERWIVGGHSAGAFLALA
                     YALEFPERVGGIIYLAGTDIQNDRAWHVAYSDARDTVGELVPEFDIEFNPDVNREATE
                     SARAYCRDPFLLKRLSQLQAPMLAVSGREDIRPVWPVEQLVHVMPSAGIVTLPEAGHY
                     IWLTHAESLKAVLRGFLQPLIQEEL"
     gene            complement(4178..4987)
                     /locus_tag="VGR29_08955"
     CDS             complement(4178..4987)
                     /locus_tag="VGR29_08955"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV2073754.1"
                     /translation="MGKKKDAKKKDTKKKEKRKEKKGEVAQPAALSIVTDEGQHGQQE
                     APVASASQNGHAEPQVMPNGQNAHVDAPAASIVPVAEAHAPTTPSLRERQSQLKLKYK
                     VRPDSALITSSVSSVTEEGQDPRQVRIAIDGPSGVVLDIGAHAAVGGFDELPCSGDIF
                     LASLAACQELTIRLVAAALNLPIHKLNVRVEGDWDVRGTLGVLREAPVGFTDLRMNID
                     LETDGSPERVERLLKSAERFCVVSETLKNPPALVINANVTNPQAEAVQAGN"
     gene            complement(5006..5830)
                     /locus_tag="VGR29_08960"
     CDS             complement(5006..5830)
                     /locus_tag="VGR29_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073755.1"
                     /translation="MSAITTHTTAARTGSQPRSSRRVPVPRAAIGLIVPLLLLGLWQW
                     LVEREVYTRGQLPAPLDVWRAAEQLQGADRLWENVQVSLVRVGWGFGIGAVVAIAFGL
                     LIGLSPLADSLLSPTIHALRAIPSLAWVPLLILWMGIGEEPKLTLIAIGAFFPVFTAL
                     VGGIRQIDRKLIEVGYAYGLRGIRLARSIVLPAALPSLLTGLRIGLAQAWLFLVAAEL
                     MGASTGLGFLLTDGQNSGRADIILLSIIILALIGKLTDLLLQRGERWLLRWSDSYR"
     gene            complement(5863..6909)
                     /locus_tag="VGR29_08965"
     CDS             complement(5863..6909)
                     /locus_tag="VGR29_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV2073756.1"
                     /translation="MNSINRSSTQALSTPSLDRRRFLGIALGASAAVALPGVVRHAGA
                     QDGEKPETVRVDYAYYNPSSLVLRRQGWLEEDLSADDVAVEWTLSAGSNKANEFLRSD
                     AIDFGSTAGSAALLAKANGSEIKTVYIYSQPEWTALVVAKDSDITSVDQLKGKKIAAT
                     KGTDPYFFLLQSLHEAGLKESDVEVVNIQHADGKTALERGDVDAWAGLDPHMAQTELE
                     QGSKLIYRNIDFNTYGFLNARIEFIESYPSYVERVIAQYERARKWILENPEEAAQILS
                     EEASLSLEVAQKELEERTNLENEVIPGDVHRAALEPVIPIFEAEAQLQPGADANEALD
                     ELFAPEFAAAVVEQ"
     gene            complement(6959..7765)
                     /locus_tag="VGR29_08970"
     CDS             complement(6959..7765)
                     /locus_tag="VGR29_08970"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2073757.1"
                     /translation="MAQAVSINHVTKSFFSGRAHLPVLESLSLTAQPGEIVALLGPSG
                     CGKSTILRLVGGLEAPTGGRVEIGTRPVTTTDPRCSIVFQEPRLLPWRSVADNVALGA
                     ERRRDSQVERGREVVDRLLARVGLGDCAGLYSHQLSGGMAQRVALARGLAAEPEVLLL
                     DEPFAALDALTRLSMQDLLIEAWSDRKPTVLLVTHDVDEAIYLADRIVVLGGSRPASV
                     IAQIGVPAERPRDHADPAFLAIRAQILGHLGFTHRGAAEDRSPAAVHAAD"
     regulatory      complement(7897..8005)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8118..9323)
                     /locus_tag="VGR29_08975"
     CDS             complement(8118..9323)
                     /locus_tag="VGR29_08975"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEV2073758.1"
                     /translation="MAEQLAIDGGAPVRTTPFPSWPIHGVREEELLGEVLRGGEWGEI
                     TGKKVHAFARQFASFQGARYGVCVPNGTLALELAFEALGVGHGDEIITSAYTFIATAG
                     AALTMGAKPVFVDIDPETNLIDPARIEAVTTPKTKALVPVHVGGQPADLDAVLEIGRR
                     HGIPVLEDACQAWGAAWRGTPVGAIGDAGTFSFQESKNITAGEGGILVTNDRHLFDLA
                     WRIHDCGRPPEGSSFEHEMPGRNLRMTEWQAAILLAQLERLSEAMSTRTRNAARLTAS
                     LTEISGLVAMKVDDRITSHGWHLYQMRFDPTAFGGRSRADFLTALRAEGIPCGAGYVP
                     LPYQKAIRNALRSRFGEEAVTGQPDVPRAAEAGEHTVLFVQTMLLGTDEDMDEIATAC
                     RKIQRAWSS"
     gene            complement(9639..9992)
                     /locus_tag="VGR29_08980"
     CDS             complement(9639..9992)
                     /locus_tag="VGR29_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073759.1"
                     /translation="MVTVIFGEPPCYGLYAGTGVDLYYADMKQDPVGTAASGRIYLHA
                     SRVAESVSYPVDVAVVTILEELVHVWMNVKDEDIAKFATAKLHGEIVSRDGRYLTKEQ
                     FWSELAKDAGADGER"
     gene            10485..12875
                     /locus_tag="VGR29_08985"
     CDS             10485..12875
                     /locus_tag="VGR29_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV2073760.1"
                     /translation="MSMATRTDTQAPALHPADSHDMIRVQGARENNLKDVSVEIPKRR
                     LTVFTGVSGSGKSSLVFSTIAAESQRMINETYSAFVQGFMPTLPRPEVDVLDGLTTAI
                     IVDQERLGANPRSTVGTVTDANAMLRILFSRLGQPHIGPPIAYSFNVPARRASGSMTV
                     DKGKGERIVVSDAVYHGGMCPRCEGMGSVSDIDLTQLYDDSKSLDEGALTAPGYSMDG
                     WYGRIFRGCGFFDPDKPIRKYTKRELHDLLYKEPTKIKVDGINLTYEGLIPRIQKSML
                     SKDREAMQPHIRAFVDRAITFATCPECGGTRLSEAARSSRIKGINIADACAMQISDLA
                     KWVRGLDEPSVAPLLTTLEHTLDSFVEIGLAYLSLDRSSGTLSGGEAQRTKLIRHLGS
                     ALTDVTYVFDEPTIGLHPHDIQRMNDLLLQLRDKGNTVLVVEHKPETIAIADHVVDLG
                     PGAGTSGGEVVFEGTVDGLRTSGTVTGSHFDDRASLKPSVRTLSGVLEVRGASTHNLR
                     DVDVDIPLGVLCVITGVAGSGKSSLIHGSVSGQDGVVAVDQTPIKGSRRSNPATYTGL
                     LEPIRKAFAKENGVKPALFSANSEGACPTCNGAGVIYTDLAMMAGVATVCEECGGKRF
                     QAEVLTYLLGGKDISEVLAMSVTEATEFFGSGPAKTPAAHKILGRMADVGLGYLSLGQ
                     PLTTLSGGERQRLKLATHMGEKGGIYVLDEPTTGLHLADVEQLLGLLDRLVGSGKSVI
                     IIEHHQAVMAHADWIIDLGPGAGHDGGRIVFEGTPADLVAVRSTLTGEHLAAYVDT"
     gene            complement(12994..13752)
                     /locus_tag="VGR29_08990"
     CDS             complement(12994..13752)
                     /locus_tag="VGR29_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2073761.1"
                     /translation="MVTSVLALNDISKQFGSGEARVDALMHITMRVDPGELVALIGPS
                     GSGKSTLLSIAGALLTATSGQLLLDGEDIASMSPAQRTRIRLERIGFIFQGSNLVSYL
                     TGREQLMFMAKLIKLPSSEANERVGRLLDALGMTKRAGNYPEEMSGGERQRIAIARAL
                     MNDPRIILADEPTASLDSARGRQVVEMLADEVHASDRAGILVTHDERLIDLCDRVIRI
                     EDGEITTDAPASSAAADGKHEQESPALLGKILRR"
     gene            complement(13785..14864)
                     /locus_tag="VGR29_08995"
     CDS             complement(13785..14864)
                     /locus_tag="VGR29_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV2073762.1"
                     /translation="MFLALREIRHSKLRFGLITGVIVMVSSLVFILSGLANGLATGQS
                     AALDGLPIDAMVISSGSDYQLDRSSLSSGTVETIRDQPDVTDASPFGATAANVKLEGT
                     DSVLGVSLIGVEPGSFAAPDPDEGEPAGTGDNGVVIDETLASEGVAIGDTLVTDPDGT
                     RLTVVGTVDGRSYRLAPTLFMPIGLWQETQTQADGGDASGINAVLVQGEPEALAGTIV
                     AGKSEIIENLPGYTEQALTLLLIQVFLVVIAAGIVASFFFIITLQKMPELGVMKAIGT
                     KTGYLARSLIVQVALLGLVGVILGTAIAWGVSLLIGSAVPFDLSMRQFVVFGAILLVV
                     ALLGTLLSLLRVARVDSLDAISKAG"
     gene            15415..16050
                     /locus_tag="VGR29_09000"
     CDS             15415..16050
                     /locus_tag="VGR29_09000"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEV2073763.1"
                     /translation="MSSLLGDFASWATDVIEALGYWGLAFLVALENVFPPIPSEVILP
                     MAGFLTGDGRMNFFLAVFAATVGSVAGALILYGVGYWFGERRVRSIIRRWGKWLGFKE
                     SDVDKANGWFDRHGGLAVMLCRVVPIVRSLISIPAGLRKMPIARFILYTALGSLVWNS
                     VLIGAGWILGDNWEEVEQYVGILQYLVIIVALIAAAWWIWNRIIKPRMRTA"
     gene            complement(16274..17677)
                     /locus_tag="VGR29_09005"
     CDS             complement(16274..17677)
                     /locus_tag="VGR29_09005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073764.1"
                     /translation="MPRTTTRQAPHRASARFILPVLVFVTLVMSMVATLGTPMVPTIA
                     RQMGVSLEAAQWLLTVTLLVGAVVTPITGRLADGPHRKRVVLGALLAVCVGASISALI
                     PFFAPFIFGRALQGFGLALVPMTIAVARDTLPREQARNGVALLSVTTAVGTGLGYPLT
                     GLLGERFSYQVAFGFAAVISLVGVGLVWRVLPVHAPQESHRLDLMGAVLLSLALVCLL
                     LVISQGGGWGWSSWRIIALATGSVVCGTLWVVQELRVAHPLVELRLSLNPLVMSANVT
                     ALLMGMGLFAVSSLVSRFVQAPEGAGYGFGAGLLVAGMMLMPLSAGSLASTWFSRWLN
                     DRLGPFLVLPIGSSIVAVSGMLLAFARSEVWEILLAVALLGLGISTTFAAIPALIIRA
                     VPPHETGSATGLNSVLRSVGGAIGSAASIALLSSYTPAGGRLPTDYGYTVAFLAGAVA
                     CLGSALASFLLTPRDLE"
     gene            complement(18450..19676)
                     /locus_tag="VGR29_09010"
     CDS             complement(18450..19676)
                     /locus_tag="VGR29_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073765.1"
                     /translation="MARRRTPFAPILALLSLLAVILATLISPVPSSAQTDQQPFATLN
                     ITFKGCPPGGDTSGPPAGCVETVDAPESAMVTAAPDWVQPVREAQRNADGSYTIPYVS
                     SGAPGAESLGLVNFFPQDFNYYTFDGVDILTRWYGGVDLVQGETREVTVYYWNGPGGL
                     IVPAENALVVNVSTCGDGIDPNVDPSGCVPATGDMPGVYVGTAPLRDIHMEDYLIREG
                     GTFTYSGLPAYTQAQVVVDGPLAGYAEVLVTGQAEVIEDDSATAFLLRNERRVIDVYL
                     YAPDDAGRSFTLTRETPEPGTGTLRLLLLSCPPGVIPHDDPARCTEAITDDGSATVTF
                     PESGEAVPLTGFEQDESGAYLITGVQSSVTIGGITPRDRERIASDADEISGQEIVYNV
                     EPGETRDGRLYYFDET"
     gene            complement(19821..20645)
                     /locus_tag="VGR29_09015"
     CDS             complement(19821..20645)
                     /locus_tag="VGR29_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV2073766.1"
                     /translation="MNPSTSLSGTVAFLFTDIEGSTRRWQTHRDEMGETVACHDALLR
                     REIEVRQGYVFKTVGDAFCATFATAPLALAAAIAIQHGLASTDWDDTGLIRVRMAIHV
                     GAAEEREGDYFGPAVNRVARLLSAGYGGQLLLSLPAAELVRDSLPDEVGLRDMGEHRL
                     KDLARPERIFQIVSPDLPADFPPLKTVNNRPNNLPQQLTPFVGRAGDVAEVVTRLGDQ
                     HVRLLTLTGPDGTGKTRLSLQVTLASVGTITCGRSGAEANIAPAPATLMQRSTCRG"
     gene            20909..21253
                     /locus_tag="VGR29_09020"
     CDS             20909..21253
                     /locus_tag="VGR29_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073767.1"
                     /translation="MAKNRKGGGRIGAVRGRTQFQLPNGHYAKRDTTTGVILSVKADL
                     KPYKGIVKEKQLQQSLPDTTVASPVARGRRAAFPPLRAWEWSIRRHTHGDRFAALPET
                     DSARSTGHLHHG"
     gene            complement(21287..21913)
                     /locus_tag="VGR29_09025"
     CDS             complement(21287..21913)
                     /locus_tag="VGR29_09025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV2073768.1"
                     /translation="MTTRDQNMKFEGKPRLMVVIASIREGRVGKAVGEWFTGFATEHG
                     SFDITVADLKEIDLPPMTEPNHPRLKQYTQEKTKEWSAMVDAADAFVFVMPEYNYFAT
                     APLISAIDYLVQEWAHKPVGLVSYGGVSGGLRAAQSIKPLITSMNMMPLKDGVAIQSV
                     SSQINGETGKFVPTDSHESSATTMLNALVQWEKAMHQFRVPSTVAAQV"
     gene            complement(22098..23348)
                     /locus_tag="VGR29_09030"
     CDS             complement(22098..23348)
                     /locus_tag="VGR29_09030"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEV2073769.1"
                     /translation="MSVFDESMSRRAFGKATVAAAAGVALAAPSLKAALAQDSATPAA
                     ADEGMGPPPLPEGATVVAEGLFNPRFLAFGDDGTLYITENGVGGDEVLPGPGGTNEGE
                     GEATVVATPMGTPVEAPPQPPSTRGYTGQITAVAPDGTQSVIASGLASYSDGAGPVGI
                     ALGAKAVYFSIGGIAVGAGMPPLQEENTVNVLDLASGEVSVIAELGPYEEANNPDGTD
                     VNPNLYELAVNPDGALLVTDAGGNAVYSVNSETGEFALLAVVPDLTELTGAEPETDAG
                     PRQPVPTGIAVADDGTTYVGLLNEFWPEGSPAVLTLEEDGTFAPVGGPLSFNVGITVG
                     PDGMIYASELFTLVEGSEEPGPGRVVRMSTDGEIEVVLEGVMMPHGIAFDADGNLYVA
                     INSLISGPGMPAGQVIRIDGVAAA"
     gene            23748..24596
                     /locus_tag="VGR29_09035"
     CDS             23748..24596
                     /locus_tag="VGR29_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073770.1"
                     /translation="MGNAMLRAAMLLTFLLGLASALVGLAQAQGDGVPMSEMPVQVLY
                     CEEDPGPVNPGGGRAISPDTLRNEFDCRPGAGVALTFSNQEMGWFARCDMDAEGECTV
                     ETPVGPEVELDVAIHMATVEPGYAPEQPIGTAVNYTEFAGYGVVLLPVGETTPAPNAE
                     RQTLAVNVATCKDGSNAAGCERQPAGALVQASAGEATAKGYPWLATNDEGWVSFDRAG
                     LKGDSIDLMLQTGTEPRFACTDVESGKRLEAEWIEGREGNFIRLTPTSAGEITCDVTL
                     LGEVGS"
     gene            complement(24665..25222)
                     /locus_tag="VGR29_09040"
     CDS             complement(24665..25222)
                     /locus_tag="VGR29_09040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2073771.1"
                     /translation="MQPYAAEVLRSLGYEGTGETHYRLNAQALTGDLSTPILPDGVSV
                     RSMRDDTGDFAARVEVHRSVWAPSKLTLEGYERLRTKPLYRGDLDLVVETAEGDLAAY
                     CIVWWDPVTKAGEFEPVGTVDCFRGRGFGKAVLIEGLRRLRDLGAGYATVISGMGPES
                     EPARRLYASAGFEPVFTFDQWKKPV"
     gene            complement(25259..25591)
                     /locus_tag="VGR29_09045"
     CDS             complement(25259..25591)
                     /locus_tag="VGR29_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073772.1"
                     /translation="MSLTWRRFVDPDDTVALITLMQRMTPWNEQGVGWLHPGDVVWRL
                     YQNLSTKPEDEVRIVANTSGTPVALVEMITPATYCIHMPAEADNPEDVIRFATAQAEQ
                     ELRAGFVR"
     gene            complement(25766..26740)
                     /locus_tag="VGR29_09050"
     CDS             complement(25766..26740)
                     /locus_tag="VGR29_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2073773.1"
                     /translation="MDRTPGTQGTEMDLLIRPLRTDELAAADVVRRLAFGTFMGLPDP
                     LSFRGDANPVRTRYLADPAGGFAAEMCGDLVGTNFVVDWGSVGFFGPVSVRPDLWDRG
                     IARRLVSAVLDEFARRGTRHIGLFTFGHSPKHVALYQRFGFWPRFPTAIMRKPVGSAS
                     TQTEWSAFSELPAGRQPACLEECRKLADAVYPGLDLSRDMVATARHGFGDTVLLLDGG
                     TLVGFAVCHCGPETEAGSGICYVKFGAVRPGSAAASTFDRLLTACEALAVAQGLARLE
                     AGVNTARREAYRRMLESGFRTNVLGLAMDRPDKPGYDRPDVYVIDDWR"
     gene            complement(27057..>27322)
                     /locus_tag="VGR29_09055"
     CDS             complement(27057..>27322)
                     /locus_tag="VGR29_09055"
                     /inference="COORDINATES: protein motif:HMM:NF015551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEV2073774.1"
                     /translation="NVAGAIPSAVPLSLLFAVAGLALISVLSNALTEMVKGPLRLGPL
                     FAFVVASSELSLGGFGSAFWALVIGMVVTLLLEHDEWRSVRSA"
BASE COUNT         5242 a         8827 c         8442 g         4811 t
ORIGIN      
        1 ggtgttgacc ttccttgaaa agcacttcga ctgggaatgg catcgcgagg cgtcggatat
       61 cttcaattcg ctctataacg gcgatggccg caacgtcggc atgctgatcg tgacgcaggg
      121 ggacgagatc cttggatacg cgcagcaccg gggcgagcgc ttcgggccat tcggtgtgcg
      181 ccccgatctc cggagcaagg ggattggccg cgtgctgctc acgcatacgc tgcggcagat
      241 gcgcgccagt ggcttccatg ttgcctggtt cctctggacc ggcgacaatg ccgcccggct
      301 ctatgcccgg tatggattcc acgaggtccg tcgcttcgcg gtcttgcgca agacattgac
      361 gtagaacgcg acgaccactc cgtacggtcg ataaaggcca aaggtcgaca tggtgccggc
      421 ccctacgagc taccaattga tcccgaaagg aacacgttgc catggcgagc aattctgaca
      481 actgcatcat ggtcgtcggc gggcatgccg ccgatgcaga ggtaatgggc ggcgcatcag
      541 tcctcaagca tgtcgacgcc ggctggcgcg ccgtcaacct ccacctcact gggggcgaga
      601 agggccacaa gagcctctcg ccggaggaat acctggagat caagaaggga gaggctgcgg
      661 aagccgccaa gcgactcggg gcagaatcga tcttcctttc ttacaaggat ggggaactgc
      721 ctgtcgacgc ggaggtgcag atggcaatcg ccgacgtcat ccgcgagtac aagccaacga
      781 tcatcctcac ccactggaag ggcagcattc accgcgatca tacgaatacc tacgagaacg
      841 tcctggcgtc gcttttcttc gccgggcttc gtaccttcga gcggaagtac ccgccgcatt
      901 gggtgagcca tatctatttc gcggagaact gggaagatga ggaaggcttc gtgccggagg
      961 tctacctcga caccaccgat gtctttgacc actacatcca cgcgatccac gcctacagcc
     1021 tcttcagggg cgaggcggcg aagttcccct atgagcaatg gtaccggggc gcgagcatga
     1081 tgcgcggcgc ggaggtcggc tacgagcagg ccgtggcact gatgaaccac cgaacctatt
     1141 acggctcccg gaagaaggcc ggacttctcg gcccggagga caagcgatga gtgcccacgt
     1201 cacgaccggt cttgaggtac tgctcgacga gggacatccc ctggtcgaag gcaggcgtat
     1261 cgggctggtc accaaccaca cggcgattga ccgggacctg cgatcggtga ttgacctgct
     1321 ccataccgat tcccgcttcg aacttgccca tctctacggg ccggagcacg gggtccgtgg
     1381 cgacgtccag gccggggtcc atgtcggcag ggctatcgac ggggagaccg gtctccccgt
     1441 cgatagcctc tacggcgaga cgagaaagcc gacgccggag atgctcgacg gcgtggacat
     1501 ccttgtcttt gatatccagg atgccggtgc ccgcttctat acctatatct cgacgatggt
     1561 cctgtcgatg gagggggcga gcgaggccgg tatcccctac gtcgtgctcg atcgcccgaa
     1621 cccgatcacc ggtgtccacg tcgaagggcc aatgctggac ccgcaattcg agtcatttgt
     1681 cgggattcac ccgatcccaa cccggcacgg gctgacggta ggggagctgg cgcgacttgt
     1741 cgccgcggat cgtggctggc ctgagccaac cgtgatccgg atgcaggggt ggcagcgcga
     1801 catgtggtac gacgagacgg gcttgccctt cgtccaggcg tcgccgaacc tgccgacgat
     1861 ggactcactg acggtctaca caggcacctg tatcttcgag gcgacgaccc tctcggaagg
     1921 acgcggcacg acccgtccct ttgagtacat cggcgcgccg tggatcaacg cgaccgagct
     1981 ggtcgcggag ttgcgatggc gcgacctcgc tggagtgggg ttccgccccg cctcgttcgt
     2041 gcccatgttc tcgaaacacg agggcgagac gtgctcgggc gtgcagctcc acatcctgga
     2101 ccgtgaggcc ttccggcccg tcgaggttgg agttcatctc gtgcacgcca tcaagcacct
     2161 gaacggctcg cagttcgcgt ggcgtcctgg tagtgcgacc ggtatgtcgg cgaatcttct
     2221 gtatggcagc gaggttctcc acgcgatgat cgacgacgat gcctcaccgg aggagatcat
     2281 ggcgacgtgg gacgacggac aggacgcgtt cagggagaaa gctggagcgg cgtacctcta
     2341 ttgatgccag agcacgggga gtcgttgcgg caggtggaac gacaaagcca cgaccatctg
     2401 gttgcgtccc gtcattcaga gcccgcaggg cgatgaatcc caggcgaagc catcgttctg
     2461 gttggctcgg cttctatgtt tccttgccca gggagcttcg tgcggcacac gcgcttcgcg
     2521 caggattctg taggctgagg ctgcaaggca aacagcgcca gcgagcttat ggccgagccc
     2581 tcagagcgag cctttgcttc gctctgaatg acgagacgca agcggcgtgg gcgctcttca
     2641 cctaccatgg tcggctatga ggacatccag tgaaacaggg attccatgaa cgatcctgcc
     2701 cctccccaga tgagcgacga tgcggtccag cgtggttctg gaaagacgtg ggacgagtgg
     2761 cgcgcgatcc tcgatgcatg gggcgcgaca aacaagccgc atgacgagat cgcccaatac
     2821 ctggtggagg atcacggtat cgatggctgg tgggcccagg gcgtcaccgt ggggtacgag
     2881 cggttgatcg gccgcagggc tgttgggcag cgagcagacg gcacgttctc caccagcgcg
     2941 agcaggaccg tcctggccgg gatagaggag cacttcgtcg cgtggaccga cgatacggcc
     3001 cgggacgcat ggctcactcc cggcatcctc agcctccgga cggcccagga acatcgctcc
     3061 gcccgcttcg acgacaacga atatggcggc attgtcgcgc tctacttctc gagcaagggc
     3121 gaaggcaaat cctcggtgaa catccagatc gagaagctcc cctccccgga cgccatcccc
     3181 gagcgcaagt caatctggaa agcacgtctg gacgatctcg cgacgtatct ggagaatgcg
     3241 cgctcaaccg attgagaagg ggaagccggg gtgaactata gctcttcctg gatcagtggc
     3301 tgcaagaatc cccggagcac cgccttgagg ctttcagcat gggtcagcca gatgtagtgg
     3361 ccggcttcag ggagcgtgac gatgccggcg ctcggcatca cgtggacgag ctgctcgacc
     3421 ggccacaccg ggcggatgtc ctcccttccg gagacggcaa gcatcggggc ctggagttgc
     3481 gaaagtcgct tcagcagaaa ggggtcgcgg caatacgcac gtgcgctctc ggtggcctca
     3541 cggttcacat ccgggttgaa ctcgatgtcg aactccggca ccagctcacc taccgtatcc
     3601 cgggcatcgg aatacgcgac gtgccacgcg cggtcgttct ggatgtcggt accagcgagg
     3661 tagatgatcc caccgacgcg ttccgggaac tccaacgcat acgcaagggc cagaaaggcg
     3721 ccggccgagt ggccgccgac gatccagcgc tcaacaccca tcgcttcgcg tatcgcgtcc
     3781 atatcggcga gggtcgtcgc aagatcatac ggaccggtgc tgccggatcg cccgcagccg
     3841 cgcggctccc accagacgac gcgaaccatg tcgtcgatca gccccgagac cggagcaagg
     3901 tagtcgccgc agccgggacc accagggcag agcaggactg gatagccgga tccgtggtcc
     3961 gccacccaga gctccacccc gtccacagct agccaaccct ctggcatcgc tccctcctca
     4021 ccctccgtgg gacgatagca cgatcaaggg ccagtcatga agcgagtggt ggagacaacc
     4081 gaagcccatc gcaactcgat ccatgagaaa gcggcaaaag aataagcagg gcaggcatta
     4141 tgcccaccct gctctcactg cctccgccta aagaccgtta gttgcccgcc tgcacggcct
     4201 cggcctgggg attggtaacg ttcgcattga tgacgagtgc cggcgggttc ttcagcgttt
     4261 cgctgacgac acagaagcgc tccgctgact tcagcagccg ctcgacccgc tctggcgagc
     4321 catcggtctc cagatcgatg ttcatgcgca gatccgtgaa cccgaccggc gcctcgcgga
     4381 gcacccccag ggtgccgcgc acgtcccaat cgccctcaac ccgcacattg agcttgtgga
     4441 tcggcaggtt gagcgccgcc gcaaccagac ggatcgtcag ctcctggcag gccgccagtg
     4501 aggcgaggaa gatgtctccc gagcagggaa gctcgtcaaa gccgcccacg gcagcatgcg
     4561 cgccgatgtc cagcacaacg ccgctggggc catcgatcgc gattctgacc tgtcggggat
     4621 cctgcccctc ctccgttacg ctggaaaccg agctggtaat cagggcggaa tccgggcgca
     4681 ccttgtactt cagcttgagc tggctttgcc gctcacgcag tgagggggtt gttggcgcat
     4741 gcgcctcggc aaccgggacg atggacgccg ccggtgcatc gacatgagcg ttctggccat
     4801 tgggcatcac ctgaggctca gcgtggccat tctggctggc cgacgccacg ggcgcttcct
     4861 gctggccatg ctgcccctca tcggtgacga tggacagcgc ggctggctgg gcgacctcgc
     4921 ccttcttctc cttgcgcttc tcctttttct tggtgtcctt cttcttcgcg tctttcttct
     4981 tgcccatcag gtttccccca aaacgttaac gatacgaatc cgaccatcgc agcagccagc
     5041 gctcaccccg ctgcagcagc agatcggtga gcttcccgat cagcgcgagg atgataatgc
     5101 tcagcaaaat gatatcggcg cggcccgagt tctggccatc agtgagcagg aaaccgagcc
     5161 ccgtcgatgc gcccatcagc tccgcggcga cgaggaagag ccacgcttgc gcgaggccta
     5221 tccgcagacc cgtcagcagc gacggcagcg ccgccggcag gacgatggat cgcgcaagcc
     5281 ggatgccgcg caaaccatac gcgtagccaa cctcaatcag cttgcgatcg atctggcgaa
     5341 tgccgccgac aagcgcggtg aagaccggaa aaaatgctcc gatcgcgatc aaggtcagct
     5401 tcggctcctc accaatgccc atccagagga tcagcaacgg cacccatgcc aatgagggga
     5461 tcgcccgcag cgcgtggatc gttggcgaga gcagggaatc ggccagtggt gacaaaccga
     5521 tcaggaggcc gaaagcgatc gcgaccaccg cgccaatccc gaacccccaa ccgacgcgta
     5581 ccaaactgac ctggacgttc tcccagagtc gatcggcgcc ctgcagctgc tctgctgctc
     5641 gccagacatc aaggggcgca gggagctgtc cccgggtgta gacctcgcgc tccacgagcc
     5701 actgccagag gcccagcagg agcaacggga cgatgagccc gattgcggcg cgcggcaccg
     5761 ggacgcggcg ggacgaacgg ggttgggacc cggttcgcgc cgcggttgtg tgggtagtga
     5821 ttgcgctcat ctggattcct ccctctgcgg gtatggggga tgctactgct cgaccacagc
     5881 ggccgcgaac tcgggcgcaa agagctcgtc cagcgcttca ttcgcgtcag cacccggctg
     5941 cagctgtgct tccgcctcga agatcgggat gaccggctcc agtgcggcgc ggtggacgtc
     6001 gcccgggatt acctcgttct ccaggttcgt ccgctcctcc agctccttct gcgccacctc
     6061 aagcgagagg cttgcctcct cgctcaagat ctgcgcagcc tcctccggat tctccaggat
     6121 ccacttccgt gcccgctcgt actgcgcgat cacacgctcg acgtaggagg gatagctctc
     6181 gataaactcg atccgggcat tgagaaagcc gtaggtattg aaatcgatgt tgcggtagat
     6241 cagcttcgag ccctgttcga gctcggtctg ggccatgtgt ggatccagac cggcccaggc
     6301 gtcgacatca ccgcgctcca gggcagtctt accgtcggcg tgctggatgt tgacgacctc
     6361 gacatcggat tccttcaggc cggcttcgtg cagggactgg agcaggaaga agtacgggtc
     6421 ggtgcccttg gtggcggcga tcttcttgcc cttcagctga tcgacggacg taatatcgga
     6481 atccttggcg acgacaagcg ccgtccattc cggctgggaa tagatgtaga ccgtcttgat
     6541 ctcggagccg ttggcctttg ccagcagggc cgctgagccg gcggtcgagc cgaaatcgat
     6601 ggcatccgag cgaaggaact cgttggcctt gttgctgcca gcactgagcg tccactccac
     6661 ggcgacatcg tccgccgaga gatcctcttc cagccagccc tgacggcgaa gcacaagact
     6721 gctcgggttg tagtaggcgt agtcgacgcg aaccgtttcc ggcttctcgc catcctgcgc
     6781 cccggcgtga cggacgacac ccggcagggc cacggcggct gacgcaccaa gcgcaatgcc
     6841 gaggaaacga cgtcggtcaa gggacggtgt actgagagcc tgggtgctgg agcgattgat
     6901 ggagttcatg ggtgattgtt tccttttcta ataaaacctg tctaacaaaa cgacgtggtt
     6961 agtcggcggc atgcaccgcc gccggggacc gatcttccgc tgcgccccgg tgagtgaaac
     7021 cgaggtggcc gaggatctgg gcgcggatgg ccaggaaggc tgggtccgca tggtcgcggg
     7081 ggcgctcggc agggacaccg atctgggcga tcaccgacgc tggccgcgag ccgccgagaa
     7141 cgacgatccg gtcagcgagg tagatcgcct catcgacatc atgggtgacg agcaacactg
     7201 ttggcttccg gtcgctccag gcttcgatca gcagatcctg catgctcagc cgggtcaggg
     7261 catcgagcgc ggcaaagggc tcgtccagca gcagcacctc cggctcggcg gcaagaccac
     7321 gtgccagggc aacgcgctgc gccatgccac cggagagctg atgcgagtag aggcccgcac
     7381 agtcgcccag cccgacccgg gcgagtaacc gatcgacgac ctccctgccc cgctcgacct
     7441 ggctatcacg gcgccgttcg gcaccgagcg cgacattgtc ggcgacggac cgccatggca
     7501 ggagccgcgg ctcctggaag acgatcgagc agcgtgggtc tgtggtcgtc accggacgcg
     7561 tgccgatctc gacccggccc ccggtgggtg cctccaggcc accaaccagg cgcaggatcg
     7621 tcgacttgcc acagccgctg gggccgagca gggccacgat ctcgccgggt tgggcggtga
     7681 gcgacagact ctccagcacc gggagatgag cccggcccga aaagaacgac ttggtaacgt
     7741 ggttgatgga aactgcttgt gccatgcgct tgccccgtgt ttgccttcgt caccggtgct
     7801 tcatttcgga agctggcaac gaaaaacgcc tcctgatcgt cggtggacga tggggagacg
     7861 tggacaacgc gcgacatgca ggcgtggggc caaatcacct catcgtccag catgcttgct
     7921 gtcggcatta gcacctgccc ggcgactctg ccgggtggtt gctggagctt cacagggccg
     7981 atccctctgc tcctcttgat gagtcgtgat tcacattcag ttgttgtacc ggacaatagc
     8041 ggaagctgct atctcctgtc aagttgcgtg aaatgtctac cggttctcac aacaatcccc
     8101 cgggcagccg cgctctttca ggatgaccat gcccgctgga tcttccggca cgccgtggca
     8161 atctcgtcca tgtcttcatc ggtcccaagc agcatcgtct gcacgaacag gactgtatgc
     8221 tcaccggcct ctgctgcgcg agggacatcc ggctgaccgg tcactgcctc ctcaccaaaa
     8281 cgcgaccgca acgcattccg gatcgccttc tgatacggta atggcacgta cccagccccg
     8341 caggggatac cttctgccct gagcgccgtg aggaagtcgg cacggctccg acctccgaac
     8401 gcggttggat caaaccgcat ctggtagaga tgccagccgt ggctggtaat tcgatcatcc
     8461 accttcatcg cgaccaaccc ggaaatctcc gtgaggctag cggtcagccg tgcagcattg
     8521 cgcgtccgtg tgctcatcgc ttccgacaat cgctcaagct gggcgagcag gattgctgcc
     8581 tgccattcgg tcatgcggag gttgcgccca ggcatctcgt gctcgaagct gctgccctcg
     8641 ggcggacgac cacagtcgtg gatccgccac gcaagatcga aaaggtgacg gtcgttcgtg
     8701 acgaggatgc cgccctcccc tgccgtgatg ttcttgctct cctggaagct gaacgtcccg
     8761 gcgtccccga tcgccccgac gggcgtcccc ctccaggccg ctccccatgc ctgacacgca
     8821 tcttccaaca ccggaatgcc atgccgtcgg ccgatctcca gcaccgcgtc cagatcagcc
     8881 ggctgaccgc cgacgtgaac cgggacaagc gcttttgtct tcggcgttgt cactgcttcg
     8941 atccgcgccg gatcgatgag gttggtctcc gggtcgatat cgacaaagac gggtttcgcg
     9001 cccatcgtca atgctgcgcc cgccgtcgca atgaaggtgt aggccgaggt gatgatctcg
     9061 tcgccatggc ccacgccgag cgcctcgaat gcaagctcca gcgccagcgt gccgttcggg
     9121 acacagacac cgtatctggc accctgaaac gacgcgaact gccgggcgaa ggcgtgcacc
     9181 ttcttcccgg tgatctcacc ccactctcct ccccgaagca cctcaccgag aagctcttcc
     9241 tcccggacac catgaatcgg ccacgacgga aacggcgtcg tccggaccgg cgctccccca
     9301 tcgatcgcaa gctgttccgc catgaacgtc tctcctggtc gtcatcgtcc cgttattcat
     9361 gccgggcagc gtctcactac ccccgaagga cgagagcagg gctttcttgc tgatgctgtg
     9421 cgtgacgcgc tgagccgaat tacccgggcc ttgggcacct aacatatgac ctacacgctg
     9481 attgcgacca agttagtcga actgatccag gggatcagtg gctttgccgc ggtctatgag
     9541 cacgacgccg atgagctaaa ggcgtctggg agattaccca gccgacgagt gggatcttcc
     9601 tcaaggttaa gcgcaaagtg ccaatcgaga tggtcgaact aacgctctcc atccgctccc
     9661 gcgtccttcg ctaactcact ccagaactgt tccttcgtga ggtaacgccc atcacgggag
     9721 acgatctcac catgtagttt tgccgttgca aacttggcaa tgtcctcgtc ctttacgttc
     9781 atccatacat ggacgagctc ttcgaggata gtgacaactg cgacatcgac ggggtagctg
     9841 acagactcgg caacccggct ggcatggaga tagattcgcc cactcgccgc cgttcccact
     9901 ggatcctgct tcatatcggc atagtagaga tcgaccccag taccggcata gagcccatag
     9961 caaggcggct caccgaagat cactgtcacc atccagaagt gctcagtgcc ggggcgatca
    10021 aattaacagc gtccgccaat gcttaagcca gtcgctcttt gacgtctggc tcaagttcga
    10081 tgctgtaggg gatgatcagc ccccgtaggt ctggatgaac atacttacgt agctccatat
    10141 cagattgtga aagttggaat gattgctcag tcatatctct tcctcccacg atggataacg
    10201 gctccccgct caattcctag aataggagtg gcggctacct atcgctacac ctacacgggt
    10261 gaagggtaaa tacacctgcc cctgcacggg atcatcaccg atccagccaa caggggtgtc
    10321 aggttcatag atccgacatc gctgtcagac accccgactc tacacccgcc gggaaggagg
    10381 agaaggaggc caaagcgaag tatcgttaac aactcgatat ggcagcaggc gttctttctc
    10441 aaatcagcgt tcgcaccgaa tttatttcga taggaagaga cacgatgagc atggccacga
    10501 gaacggacac gcaggcgcct gcgctgcacc ctgccgacag ccacgacatg atccgcgtgc
    10561 agggcgcgcg agagaacaac ctcaaggatg tcagtgtcga gatcccgaag cgacggctga
    10621 cggtgttcac cggcgtctcc ggatcgggca agagctcgct ggtgttcagc acgatcgccg
    10681 cggagtcgca gcggatgatc aacgagacgt acagcgcttt cgtgcagggt ttcatgccga
    10741 cgctgccgcg acccgaggtc gacgtgctag atgggttgac gaccgcgatc atcgtcgacc
    10801 aggagcggtt gggagccaat ccccgctcca cagtcggcac ggtcaccgat gccaacgcga
    10861 tgctgcggat cctgttcagc cggctcgggc agccccacat cgggccgccg attgcctact
    10921 cgttcaacgt cccggcccgg agagcgagcg ggtcgatgac cgtcgacaag ggcaaggggg
    10981 agcggatcgt ggtgagcgac gccgtctacc acggcggtat gtgcccgcgc tgtgagggca
    11041 tgggcagcgt gtccgacatc gacctgacgc agctgtacga cgacagcaag tcgctcgacg
    11101 agggtgcgct cacggctccc ggctacagca tggacggctg gtacggccgc atcttcaggg
    11161 gctgcggctt cttcgacccg gacaagccga tccgcaagta cacgaagcgc gagctccacg
    11221 acttgctcta caaggagccg accaagatca aggtcgacgg catcaacctc acctacgagg
    11281 ggctgatccc caggatccag aagtcgatgc tctccaagga cagggaggcg atgcagccgc
    11341 acatccgggc gttcgtggac cgggcgatca ccttcgccac ctgccccgaa tgcggtggca
    11401 cccggctgag cgaggcggcc aggtcctcca ggatcaaggg gatcaacatt gccgacgcct
    11461 gcgcgatgca gatcagtgac ctggccaaat gggtccgcgg tcttgacgag ccgtcggtgg
    11521 caccgctgct gaccacgctg gagcacaccc tggactcgtt cgtggagatc gggctggcct
    11581 acctctccct tgaccggtcc tcgggcaccc tgtccggcgg cgaggcgcag cgcacgaaac
    11641 tgatccggca cctcggctcc gcgctcaccg acgtcaccta cgtcttcgac gagcccacca
    11701 tcggcctgca ccctcatgac atccagcgga tgaacgacct gctgctgcaa ctgcgggaca
    11761 agggcaacac ggtcctcgtt gtcgagcaca agccggagac gatcgcgatc gccgaccacg
    11821 tcgtcgacct cggacccggc gccgggacgt ccggtggcga ggtggtgttc gagggcaccg
    11881 tcgacgggct gcggaccagc ggcaccgtca ccgggagcca cttcgatgac cgcgcttcct
    11941 tgaagccgtc ggtgcggacg ctgtcaggag tgctggaagt gcgcggcgcc agcacccaca
    12001 acctccggga cgtcgatgtc gacatcccgc ttggggtgct ttgtgtgatt accggcgtgg
    12061 cgggctcggg caagagttcg ctgatccacg gctcggtgtc gggccaggac ggggtcgtgg
    12121 cggttgatca gacccctatc aagggctcac gacggagcaa cccggcaacg tacaccgggc
    12181 tcctggagcc gatccgcaag gcattcgcga aggagaacgg cgtcaagccg gccctcttca
    12241 gtgccaactc cgagggcgcc tgccctacct gcaacggcgc cggcgtcatc tacaccgacc
    12301 tggcgatgat ggccggcgtc gcgacggtgt gcgaggagtg cggcggcaag cggttccagg
    12361 ccgaggtgct cacctacctg ctcggcggca aggacatcag cgaggttctt gcgatgtcag
    12421 tgaccgaggc gacggagttc ttcgggtccg gcccggccaa gacgccggct gcgcacaaga
    12481 tcctggggcg catggccgat gtcgggctcg gctatctctc cctgggccag ccgcttacca
    12541 cgctgtccgg cggcgagcga cagcggctca agctcgccac ccacatgggc gagaagggcg
    12601 gaatctacgt gctcgacgag ccaaccaccg gcctccacct cgccgatgtc gagcagcttc
    12661 tcggcttgct cgaccggctg gttggctccg gcaagtcggt catcatcatc gagcaccacc
    12721 aggccgtgat ggcccacgcc gactggatca tcgacctcgg ccccggcgcc ggccacgacg
    12781 gcggccggat cgtcttcgag ggcacacccg ccgacctcgt cgccgtccgc tccaccctca
    12841 ccggcgagca cctcgcagcc tacgtcgaca cctgacgggg gccgtcgcag acacgctccc
    12901 atgacccact cgaccaccga cgcatcggtt caaggaatgc ctcatgccgg gcgccccatg
    12961 agttgagact atcaacgtgg acgtggagca ggatcagcgg cggagtatct tgcccaggag
    13021 tgccggtgac tcctgctcgt gcttcccgtc ggcggcagcg gaactcgcgg gagcgtccgt
    13081 cgtgatttcg ccgtcctcga tgcggatgac ccgatcgcag agatcgatca gccgctcatc
    13141 gtgggtgacg agaatgccgg cccggtcgct cgcgtggacc tcgtcggcca gcatctccac
    13201 gacctgccgc ccgcgagcgg agtcgaggct tgccgttggc tcgtctgcga ggatgatgcg
    13261 cggatcgttc atcaatgccc gggcgatggc gatccgctgg cgctcgccac cagacatctc
    13321 ttccggatag ttgccggcgc gtttcgtcat accgagcgca tcgagcagcc gcccgacccg
    13381 ctcgttggcc tcggacgagg gcagcttgat cagcttggcc atgaacatca actgctcgcg
    13441 ccctgtcagg taggagacca ggttggaccc ctggaagatg aatccgatgc gctcaagacg
    13501 gatccgcgtg cgctgcgccg ggctcatgct ggcgatgtcc tccccgtcga gcagcagctg
    13561 gcccgatgtt gcggtaagga gagccccggc gatcgagagc agcgtcgact tgcccgagcc
    13621 cgagggcccg atcagcgcca cgagctctcc cggatccacc cgcatggtga tgtgcatgag
    13681 cgcgtccacc cgggcttcgc cggagccgaa ctgcttgctg atgtcgttga gcgcgaggac
    13741 gctggtgacc atggatgccg ctcctgtgtg ctgcctgtgt tccgctagcc ggccttgctg
    13801 atcgcatcaa gcgaatcaac ccgtgctaca cggagcaatg agagaagggt tccgagcaag
    13861 gcaacaacca gcaggatggc gccaaagacg acgaactgcc tcatcgacag atcgaagggc
    13921 accgcgctcc cgatcagcag cgagacaccc caggcgattg ccgtcccgag gatcacgccg
    13981 accaggccca gcagggcgac ctggacgatc aggctgcggg caaggtatcc ggttttcgta
    14041 ccgatcgcct tcattacccc aagttcgggc atcttttgca aggtgatgat gaagaagaac
    14101 gaggcgacga taccggccgc gatgacgacc aggaagacct ggatcaggag cagcgtcagc
    14161 gcttgctcgg tgtatcccgg gagattctcg atgatctccg acttgccggc gacgatcgtg
    14221 cctgcgagtg cctccggctc gccctgcacc agcaccgcgt tgatcccgga tgcatcgccg
    14281 ccatctgcct gggtctgcgt ctcctgccag agaccgatcg gcatgaagag cgtcggggcc
    14341 aggcggtatg atcgaccgtc aaccgtccca accacggtga gcctggtgcc gtccggatca
    14401 gtcacaaggg tgtcgccgat ggcaaccccc tcgctggcga gtgtctcgtc gatgaccacg
    14461 ccgttatcgc ccgtgccagc cggctcccct tcgtccgggt ccggtgcggc aaagctcccg
    14521 ggctcgacac cgatcaggga gacacccagc acgctgtccg ttccctcaag cttcacgttc
    14581 gccgccgtcg cgccgaaggg agaggcgtcc gtgacatccg gttggtcacg aatcgtttca
    14641 accgtccccg acgacaggct tgagcggtca agctggtaat cactgccgga gctgatcacc
    14701 atcgcgtcga ttggcagtcc gtcgagcgct gcggactgac ccgtcgcaag cccatttgcc
    14761 agaccggaga gaataaagac caggctggag accatgacga tgacgccggt gatcaggcca
    14821 aaccgaagct tcgagtgtcg gatttcacgg agtgccagga acatctgtgt atcaactttc
    14881 cgggtatcag acgcccctcg cctcgaacag cctgcatcag cgaatgtgca tacacagtca
    14941 gtgtctggta acctcaacta caagtcaagg ttttacaggg gcaacaatgg tccagggacc
    15001 ggggatggac gacatgcgct gtagagcaag aggagactgc ttttgccctg atacagggga
    15061 atggggcggt agggtcaccc caacgattcc aggaacaatg gcgaccagca cctgatgggg
    15121 ccgcgctcgt ctcccggaca tcaccaggat gccggccctt cgcccgtgcc atccactcct
    15181 cgcacgaaag cggagtacca ttagcgcggc cacccggatg tcccgcaatt gggcttccct
    15241 gcgtcagata gacccgccat cgcagttcca gtgctctgca tacggaccgc gtttgctccg
    15301 gcttgatccg gccacacaag acgctcgatt gaacgcacga gcggcgtcat gttctgtgac
    15361 gcccgctctt tcaggccgcg cgcctggacc gaaccttaca ggagaaccaa ctcgatgagc
    15421 agcctgctcg gggatttcgc aagctgggcg acagatgtga tcgaagcgct gggctactgg
    15481 ggacttgcct tcctggttgc gctcgagaac gtcttcccgc caattccgtc ggaagtcatc
    15541 ctgcccatgg cggggttcct gaccggtgac gggcgcatga atttcttcct tgccgtcttc
    15601 gccgccacgg ttggatcagt cgccggcgcc ctcatcctgt acggtgtcgg gtactggttt
    15661 ggtgaacgga gggtgcgctc gatcatccgg cgctggggta aatggctcgg cttcaaggag
    15721 tcggatgtcg acaaggccaa tggctggttc gaccgtcacg ggggcctcgc ggtgatgctg
    15781 tgccgggtcg tcccgatcgt ccgcagcctg atctcgattc cagcgggtct gcgcaagatg
    15841 ccgatcgcga ggttcatcct ctatacggcg ttgggcagtc tggtctggaa cagcgtcctc
    15901 atcggtgccg gctggatctt gggcgataac tgggaggaag tcgagcagta cgtcggcatc
    15961 ctgcagtatc tcgtgattat cgtggcactc atcgccgccg cctggtggat ctggaaccgt
    16021 atcatcaagc cgcggatgcg tacggcctag cgccgcatcg agccgccgga tgtggctcag
    16081 ccacgcttgc tccctgctcg ccttcattca gagcgcagca agggctcgct ggcgcccggc
    16141 cgagcctgca aggtgagaag cggagcgagc ctttttcgcc ctgagggccc ggccatgaga
    16201 atcctgcgcg aagcggtgca ggcaaccagg tgttcccaga agcatccccc tcatcctgac
    16261 tcctccggcg tcctcactcc agatcccggg gggtgagcag aaagctcgcg agggcactcc
    16321 ccaggcaggc aaccgcacca gcgaggaaag caaccgtata gccatagtcg gtggggagcc
    16381 gccctcccgc gggagtgtag ctgctgagca acgcgatgct tgccgcactg ccgatcgccc
    16441 caccgacaga gcgcagcacc gagttgagcc ccgtcgcgct ccccgtctca tgcggcggca
    16501 ccgcgcgaat gatgagggcc ggtatcgccg cgaaggtcgt gctgatcccg agcccgagca
    16561 gcgccaccgc aagcaggatc tcccacacct cgctccgcgc aaaggccagc agcatccccg
    16621 acacagcgac gatcgatgac ccgatgggca gcaccaggaa gggtccgagg cggtcattca
    16681 gccagcggga gaaccaggtc gaggccaggc tcccggccga cagcggcatc agcatcatcc
    16741 ccgccaccag cagcccggca ccgaagccgt agcccgcccc ttccggcgcc tgcacgaagc
    16801 gactcaccag cgaggagacg gcgaaaaggc ccatccccat cagcagggcg gtgacattcg
    16861 cgctcatcac caggggattc agcgagagcc gcaactccac cagcggatgc gctacccgta
    16921 actcctgcac cacccagagg gtgccgcaaa ccaccgagcc ggttgctagc gcaataatcc
    16981 gccacgagct ccatccccaa ccgccgccct gactgatcac cagcagcagg cagaccagtg
    17041 ccagcgagag cagcaccgcg cccatcagat ccaaccggtg gctctcctgc ggagcgtgta
    17101 cgggtagcac ccgccagacg agccccaccc cgacgagact gatgaccgcc gcgaagccga
    17161 acgcaacctg gtagctgaat cgctcgccga gcagccctgt cagcggatag cccagcccgg
    17221 tgccgacagc ggtggtgacc gagaggaggg cgacgccgtt acgcgcctgc tcgcggggca
    17281 gcgtatcccg cgcgacggcg atcgtcatcg gcaccagggc gaggccaaac ccctgcaggg
    17341 cccgcccgaa gataaaggga gcgaagaacg ggatcagggc ggagatcgag gcgccaacgc
    17401 agacagccaa gagcgctcca aggacaaccc gcttgcgatg cggcccatcc gccaggcgac
    17461 ccgtgatcgg cgtcaccacc gcgccgacga ggagcgtgac cgtaagcagc cattgggccg
    17521 cctccagcga gacacccatc tgccgggcga tcgtcggcac cattggcgtg ccgagggtcg
    17581 cgaccatcga catcacgagc gtcacaaaaa cgagcaccgg caggataaac cgtgccgatg
    17641 ccctgtgcgg cgcctgtctt gtcgtcgtcc gaggcattcc ggcggtgtcc ctgcacgcgc
    17701 tctcgcgctc atacccggaa gctatcgtac cgcgatggcc tgatggcgca acattgatac
    17761 gctgaaccgc catcgctgtc cgcccaggtt gcactgccag atcccacttc acttgaggaa
    17821 tatgcctgtt acgccactcg gcatcggctt cgccgggaat tctcacccct tcggcacggc
    17881 tcagggtctg gacaggctct tccctgcggt cggggtaagc atcggtggta gctttcgcag
    17941 cgctcctcta ccgccacccc ctcgtcattc tgagcggatg cgaagaatcc ccgtgcgaag
    18001 cacttccaga cttgtgcgac gcacgttcca tttcatgccg acgatgatcc tccgcgagat
    18061 ccgtctcgtc gaaccaatcc caagccaagt ctttccagcg cgggttcagc cctttaatca
    18121 gcgcaatctt cttcgcccgt aatttcgggc ggttaccatc acccaccctg ccctcctgca
    18181 tgccctgcgg gcatcggctt cgccgggggg atcctcaata ggcgattgcg taacaattga
    18241 gcggatgcag cccattcctt cggtcaggat gaaggttgag tgatgtgcca cgtgatggtc
    18301 ccatccaatg gagcccgtac ctctcgttct gcatccttgc tcatccactg ttcgtcattc
    18361 tgagcggatg cgaagaatcc ccgtgcgaag cacttaacta caggacgtcg caaacatcta
    18421 cacccatcgg ggtgctgccg tccctacggc taggtctcgt cgaagtagta gagacgaccg
    18481 tccctcgtct cgccgggctc gacgttgtaa acgatctcct ggccactgat ctcgtcggca
    18541 tcactggcga ttcgctcccg atcgcgaggg gtgatgccgc cgatggtgac cgaactctgg
    18601 acgcccgtga tgaggtaagc accgctttcg tcctgctcga acccggtcag gggcacggcc
    18661 tcaccactct ccggaaatgt cactgtcgcc gatccgtcgt ccgtgatcgc ctcggtgcat
    18721 cgtgcgggat cgtcatgggg aatgacgcca ggcgggcagg acaacaacag caggcgaagc
    18781 gtgccggttc ccggctcggg tgtctccctg gtaagcgtga atgacctccc ggcatcatcg
    18841 ggtgcgtaga ggtagacatc gatgactcgt cgctcgttcc ggagcaggaa cgccgtcgcc
    18901 gagtcatcct cgatcacctc cgcctggccg gtcacgagca cctccgcata tccggccaat
    18961 ggcccgtcca cgacaacctg tgcctgcgtg taggccggca gccctgaata ggtaaacgtg
    19021 ccaccctcac ggatgaggta gtcctccata tggatatctc gcaggggcgc ggtcccgacg
    19081 tagacccctg gcatatcgcc ggtagccgga acgcagccgg acgggtccac attgggatcg
    19141 ataccgtcgc cgcatgtcga gacattgacc acaagcgcat tttcggcggg cacgataaga
    19201 ccacccggcc cgttccagta atagacagtg acctcgcgtg tttcgccctg gaccagatcc
    19261 acaccaccat accaacgggt caagatatcc accccgtcaa aggtgtagta gttgaagtcc
    19321 tgcgggaaga agttcaccag ccccaggctc tccgcaccgg gggctcccga ggagacgtag
    19381 gggatcgtgt aggagccatc cgcgttgcgc tgggcctcgc ggacaggctg cacccagtcg
    19441 ggcgcggcgg tcaccatcgc gctctccggc gcatcgaccg tctcaacgca cccggcggga
    19501 ggcccgctcg tatcgccgcc tggtgggcag cccttgaacg tgatgttcag cgtcgcaaat
    19561 ggctgctggt ccgtctgggc agaggacggt acagggctga tcaacgtggc gaggatgacg
    19621 gcgagcaacg acagcagggc gagaatcggt gcgaatgggg tccgtcgacg tgccatgatc
    19681 agctcctcca gcgggccagg tcatccgcga actatcgggt tttgcatcga cgctaagacc
    19741 tgagccggat aatccagtcc ggggatgcag ccacgatggc aagatcgggg ccagtggagg
    19801 gactacgcga gggcacgagg ctatccccga cacgtcgaac gctgcatcaa cgtcgcaggg
    19861 gcaggcgcga tgttcgcctc tgcgccagac ctgccgcatg tgatcgttcc aaccgacgcg
    19921 agggtgacct gaagtgagag gcgcgtcttg cccgtcccgt ctggcccggt cagggtcagc
    19981 agtcgcacat gttggtctcc gagccgtgtc acgacctcgg ccacatcccc ggcacgccca
    20041 acgaacgggg tcagctgttg tggcaagttg ttgggacgat tgttgaccgt cttcagtggc
    20101 ggaaagtcgg ccgggaggtc cgggctgacg atctggaaga ttcgttccgg gcgggccagg
    20161 tccttgagac ggtgctcccc catgtcccgc agcccaacct catcgggaag gctgtcccgg
    20221 accaattctg cggcagggag ggagagcagg agctggcctc cgtacccggc ggagagcagg
    20281 cgagccaccc ggttgaccgc aggaccgaaa tagtccccct cccgctcctc ggcagcccct
    20341 acatggatgg ccatccgcac ccggatcagt cccgtatcat cccaatctgt cgaggccagg
    20401 ccatgctgga tagcgatagc cgcggccagg gcaaggggtg ccgtggcgaa cgtcgcgcaa
    20461 aaggcatcgc ccaccgtctt gaagacgtag ccctggcgga cctcgatctc tcggcgcagc
    20521 agggcatcgt gacaggctac ggtctcgccc atctcatccc gatgggtctg ccagcgcctg
    20581 gtcgacccct cgatgtcggt aaagaggaag gccaccgttc cgctgagcga cgttgatggg
    20641 ttcatagcgt gtcccctgga aaggccagat tcgttgtgtg ccccttgtgg agaggatggt
    20701 acagcaagga gagccggcca ggcagacacc cccacacgac atcgacgagc acgctcgcac
    20761 ggggcctccg ggcatcaggg accgtgagac tatccaccct atttaccttg attatgttcg
    20821 ccagttctcg tacaatttat ctacctacgt aaagtaagta ttggtgcata tatcaataat
    20881 gtgcggggcc tggaaggaca gcattgggat ggcaaagaat cggaagggtg gcggccgcat
    20941 cggtgcggtc cggggacgga cgcagttcca actgccgaac gggcattacg ccaagcgaga
    21001 tacgacgacg ggcgtgatcc tcagcgtgaa ggccgatctc aagccctaca aggggatcgt
    21061 caaggaaaag cagctgcagc agtcgttacc ggataccacc gtagccagcc ctgttgcgag
    21121 gggcaggcgc gcggccttcc cgccgcttcg tgcgtgggag tggagcatca gacgacatac
    21181 gcacggggac cgatttgcgg cgctgccgga gacggattcg gcgcgtagca ctggccacct
    21241 gcatcacggc taacgaccag agtcgggtgg tatagctgac ggaaaatcac acctgcgctg
    21301 ccaccgtgga gggtacgcgg aactggtgca tggccttctc ccactgcacc agcgcattga
    21361 gcatggtggt ggcggacgac tcatgcgagt cggtcggcac gaacttgccc gtctcgccat
    21421 tgatttggct cgacacagac tggatggcga cgccatcctt gagcggcatc atgttcatcg
    21481 aggtgatcag cggcttgatc gactgtgccg cccgcaggcc accggagaca cccccgtagg
    21541 agacgagccc gaccggcttg tgcgcccact cctgcaccag gtaatcaatc gcgctgatca
    21601 acggtgcggt cgcgaaatag ttgtactcgg gcatcacgaa gacaaacgcg tcggcggcat
    21661 ccaccatcgc gctccactcc ttcgtcttct cctgcgtgta ctgtttcagg cgcgggtgat
    21721 tgggctcggt catcgggggg aggtcgatct ccttgaggtc ggcaacggtg atgtcgaacg
    21781 atccgtgctc cgttgcgaag ccggtgaacc actcccccac ggccttgcca actcgcccct
    21841 cgcgaatact ggcgataacc accatcaggc gcggcttgcc ctcgaacttc atgttctggt
    21901 cacgtgtcgt cattgatctc tccatcggcg gtgcctgcgg cagccaaacg acttccggct
    21961 acgggcaata gtgtcaacac gctcccgcaa gatgaggtcc ggcacctgca agagaccgct
    22021 ttgggaacac acagaggccc gggaaccatg ttcccaggcc tctggtcttc gtcttctcct
    22081 tgtcgcgctg tctctctcta ggcagcggcg acaccgtcga tgcggataac ctggccggca
    22141 ggcatgcccg ggccggagat caggctgttg atggcgacat agagattgcc gtcggcgtcg
    22201 aaggcgatac catgcggcat catcacgccc tccagcacaa cctcgatctc gccgtcggtc
    22261 gacatgcgca cgacgcgtcc aggtcccggc tcctcggagc cttcaaccag ggtgaagagc
    22321 tcgctggcgt agatcatgcc atccgggccc accgtgatac cgacgttgaa cgagagcggc
    22381 ccaccgaccg gggcgaaggt gccatcctct tccaatgtga gaacggcggg tgatccttcc
    22441 ggccaaaact cgttcagcag cccgacgtag gttgtaccgt cgtcggcgac cgcgataccc
    22501 gtcggcacgg gctggcgtgg gccagcatcc gtctccggct ccgcaccggt cagctccgtc
    22561 aggtccggaa cgactgcgag aagcgcgaac tcaccggttt ctgaattgac cgagtaaacg
    22621 gcgttgccac cggcatccgt caccagcaat gcaccatccg gattgaccgc cagctcgtaa
    22681 aggttcggat tgacgtctgt gccgtccggg ttgttggctt cttcgtaggg accgagttcg
    22741 gcgatgacgg aaacctcgcc cgaggcaagg tcaagaacgt tgacggtgtt ctcttcctgc
    22801 agcggcggca taccggcacc aacggcgatg ccgccgatgc tgaagtagac cgccttggcc
    22861 ccaagggcga taccgaccgg gccggcgcca tcactgtagg aggcgaggcc gcttgcgatg
    22921 accgactggg tgccatcggg cgcgactgcg gtgatctgcc cggtgtagcc tcgggtggac
    22981 ggcggctgag gcggtgcctc gaccggcgta cccatgggcg ttgcgacaac ggtcgcctct
    23041 ccctctccct cgttcgtgcc acccggcccc gggaggacct cgtcgccacc gacaccattc
    23101 tcggtgatgt agagtgtgcc atcgtcacca aaggcgagga accgcggatt gaagaggcct
    23161 tccgccacca cggtcgcacc ctccggcagc gggggaggac ccatgccctc atcggctgcc
    23221 gcaggggtcg cggaatcctg cgcgagcgct gccttcaggc tcggcgccgc cagggcaaca
    23281 cccgctgccg ccgctacggt cgccttgccg aacgctcgac gtgacatcga ctcatcgaaa
    23341 actgacatcc cttgctcctg tcacgatcca catggatcga tcggtaccta tccggaagca
    23401 ccagtcgcga aaaacgaacc cttcgcccgc gaggtactga ccggtacgac atctagatgg
    23461 cctcgtggga gactggcctc ccgagcaagc aaattgcttt gcggacgcgc cgggaacgtt
    23521 gcgccatatg tcaggattcc aggtcccatt accatcctac ggataaaacg acatgtgtga
    23581 atatttggtt ccacatagtt ctgtggtttt cggccacttc ctccgtcttc cccacttatc
    23641 gtcatcattt ggacttcccg gggtgccggg cgaagccgtt ccggcttgtg ccattgccgt
    23701 atatagttaa gcaagtgctg ttgtacggac acatgaggga cgagaggatg ggcaatgcaa
    23761 tgctgagggc ggcgatgctg ctgaccttcc tgttgggact tgcatccgcg cttgtgggcc
    23821 tggcccaggc acagggtgac ggcgtgccaa tgagcgagat gccggtgcaa gtcctctact
    23881 gcgaggaaga ccccggcccg gtcaacccgg gcggcggccg cgcaatctca ccggacaccc
    23941 tccggaacga gttcgattgc cggcccggag caggcgtcgc ccttaccttc tcgaatcagg
    24001 agatgggctg gttcgcccgg tgtgacatgg acgccgaggg tgaatgcacg gtggaaacgc
    24061 cagtggggcc ggaggtggaa ctggatgtgg cgatacatat ggcaactgtc gagccgggct
    24121 acgccccgga gcagcccatc ggtaccgcgg tgaactacac cgagttcgcg ggctacggcg
    24181 tcgtgctgct gcccgtcggg gagaccacgc cagcacccaa tgccgagcga cagacacttg
    24241 ccgtgaatgt cgccacctgc aaggatggct cgaatgctgc cgggtgcgag cgacagccgg
    24301 ccggggcgct cgtgcaggcg tccgcagggg aagccaccgc caagggatac ccgtggctgg
    24361 cgaccaacga tgagggctgg gtcagctttg accgcgcggg gctcaagggt gactccatcg
    24421 acctgatgtt gcagaccggg acggagccgc gcttcgcctg cactgacgtc gagtcgggca
    24481 agcggctgga ggccgagtgg attgaaggcc gcgaaggcaa cttcattcgc ctcaccccaa
    24541 catccgctgg cgagatcacc tgcgacgtca cgctgctcgg tgaggtcggt tcctgacaca
    24601 tgggtggagg gagtctacct tcgatgcctc aggatgaccc cgatagccgg aagggaagtc
    24661 atcttcaaac aggcttcttc cactggtcga acgtaaacac cggctcgaac ccggccgagg
    24721 catagagccg gcgtgcgggt tcactctccg gccccatgcc ggagataacg gtggcgtacc
    24781 cggctcccag atcacgcaac cggcgaagcc cctcgattag gacagccttg ccgaagccgc
    24841 gtccccgaaa gcagtccact gtgcccaccg gctcgaactc cccggctttg gtgaccggat
    24901 cccaccagac aatgcagtac gcggcgagat cgccctccgc ggtctcgaca acgaggtcga
    24961 gatcgccgcg atagagcggc ttcgtccgca agcgctcgta cccctcaagg gtgagtttcg
    25021 atggcgccca gaccgagcga tggacctcga cacgtgcagc aaaatcgccg gtgtcatccc
    25081 gcatggaccg gacgctgaca ccatcaggaa ggatcggcgt cgatagatcg ccggtcaggg
    25141 cctgcgcgtt gaggcgatag tgcgtctcgc cagtgccctc atacccgagc gaccggagga
    25201 cctccgccgc gtagggctgc acggacagta cctcagtctc gaaatgagct ggagcttcct
    25261 agcgcacgaa cccggcacgc agctcctgct cggcctgcgc tgtcgcgaac cggataacgt
    25321 cctctggatt atcggcctct gccggcatgt gaatgcagta ggtggccggt gtaatcatct
    25381 cgaccaacgc caccggggta cctgatgtgt tggccacaat ccgcacctcg tcttcgggct
    25441 tggtcgacag attctggtag agccgccaga cgacatctcc gggatgcagc cagccaacac
    25501 cctgctcgtt ccacggtgtc atccgttgca tgagcgtgat cagggcgaca gtatcatcgg
    25561 gatctacaaa cctgcgccat gtgagcgaca tcaccctgca ttccttcttc cgcaaaactc
    25621 cgggtgctct tccggctcga cggtggtggc ctgctccacg aggtagttga gcaggttggc
    25681 ccttacgtcg gctctcatcg gcccggttcg cccctgcctt cagtcgtgtg tcaacccgtc
    25741 ctctgacagg tggcactggc ggccgttacc gccaatcgtc gatgacatat acgtcgggcc
    25801 ggtcataacc gggcttgtcc ggccggtcca tcgccaggcc aagcacgttg gtgcggaacc
    25861 cactctcaag catgcggcgg taggcctcac gtcgggcagt gttgaccccg gcctccagcc
    25921 gggccaggcc ctgcgccacg gccagcgctt cgcaggctgt cagcagtcgg tcgaacgtgg
    25981 acgcagcggc cgacccgggc cgcactgccc caaacttgac gtagcagatg ccactaccgg
    26041 cctcggtttc cggtccgcag tgacaaacag cgaagccaac gagggtgccc ccgtccagca
    26101 gcagcacagt gtcaccgaag ccatgccggg cggttgcgac catgtctcgg ctcagatcga
    26161 gtccagggta tacagcgtcg gccagcttgc ggcattcctc cagacacgcc ggctgccgac
    26221 cggccggcag ttccgagaag gcggaccatt cggtttgggt gctcgccgac cccaccggtt
    26281 ttctcataat ggcagtgggg aagcgcggcc agaagccgaa cctctggtag agtgcaacgt
    26341 gctttgggct gtgaccaaac gtgaacagcc cgatgtggcg tgtcccccga cgggcgaatt
    26401 cgtcgaggac cgccgagacc aagcgccggg cgatgccgcg gtcccagagg tccgggcgga
    26461 cggaaaccgg gccgaagaac ccgacgctgc cccagtcgac cacgaagttc gtgccgacca
    26521 ggtcaccgca catctcggcc gcgaagccgc cggccggatc ggccaggtac cgggtccgga
    26581 cggggttcgc gtccccccgg aagctcagtg gatctggcag acccatgaag gtaccaaaag
    26641 cgagtcgtcg gaccacatcg gccgcagcaa gctcgtccgt tcgcaacggg cggataagca
    26701 ggtccatctc ggtcccctgg gtgcccggcg tcctatccat ctcgctacct cctcggctga
    26761 tgtgtgttcc ttccgatcaa cgccatcgcg gttagcccgg catccacctc tggagagatc
    26821 tcccaccgga aagaaggaga tcggaggctg atgaccaagc catttgcatc gcctcggtgg
    26881 accaagtggg cctattctgc agcaggcgcc gagccgcctt accgtaatgg gattgcacat
    26941 tgtgacatcg agcataaggc acagcgacag cgacggggag cgggttcctg aataacgtca
    27001 gattcctgga gaaagccaag actatcttgg cagatcagtc agttgctgat tagttatcac
    27061 gccgaccgta ccgagcgcca ctcgtcgtgt tcgaggagca gcgtcaccac catcccgatc
    27121 acgagggccc aaaacgctga gccaaaccca cccagagaca actcggatga ggcgaccacg
    27181 aaggcaaaga gaggaccgag tcggagcggc ccctttacca tttccgtcaa tgcattactt
    27241 aggacagaaa taagagccag ccctgcaacc gcgaagagca gtgacagggg gacagcagag
    27301 ggtatcgccc cggcgacgtt gg
//
LOCUS       DASYWM010000222         6593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_272012, whole genome shotgun sequence.
ACCESSION   DASYWM010000222 DASYWM010000000
VERSION     DASYWM010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272012"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /gene="metE"
                     /locus_tag="VGR29_09060"
     CDS             <1..944
                     /gene="metE"
                     /locus_tag="VGR29_09060"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV2073775.1"
                     /translation="RRAFEKGDLSQEEYETFIEGQIKAVIEHQEELGIDVLVHGEPER
                     NDMVQYFGEQLEGFAFTRNGWVQSYGSRYVRPPVIFGDVSRPEPITVHWATYAQSLSE
                     KPVKGMLTGPVTILNWSFVRDDQPRSLTCKQIALAIRDEVADLDAAGIRMIQIDEAAL
                     REGLPLRREDWQEYLDWAIECFRITASGARPETQVHTHMCYSEFGDIFGAISDMDADV
                     ISIENARSGLELLETFKREGYDKGVGPGVYDIHSPRVPPVEEIAGNLRATLTVLDAGQ
                     VWVNPDCGLKTRKDPETTAALRNMVEATKQVRAALVS"
     gene            1173..1526
                     /locus_tag="VGR29_09065"
     CDS             1173..1526
                     /locus_tag="VGR29_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV2073776.1"
                     /translation="MLKVQRKSISANAAKQRWGSLMGAVEKDGDRVVVESHGKPKVAV
                     VPVHDLERLEALDEREHREEALRRLRALEKKATDRNQELTEEDIEELSVQVGREINRR
                     ASEKQREMSRHPQQR"
     gene            1529..1957
                     /locus_tag="VGR29_09070"
     CDS             1529..1957
                     /locus_tag="VGR29_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEV2073777.1"
                     /translation="MSIDTNVFVSYYLDQRADRNPARVIQAAFDGHFELVLSETLLQE
                     LTATIESKPYLRSRVSENELNLFIQRIRGSTRITPEILGEIPSLTRDSTDDYIIVHSL
                     LDNVDVLVTGDKNLLVLGRIDNLHIVTPAAFIFLLQQGEE"
     gene            2186..3706
                     /locus_tag="VGR29_09075"
     CDS             2186..3706
                     /locus_tag="VGR29_09075"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HEV2073778.1"
                     /translation="MRDGTVVVSTSDYLNWLTCPGFSWAVMHQPELTPPEDATSRRKH
                     IAEDTVETLARTLFPDGLPIDTGDAEEDAARTRDAMTAGADIIFQATVITGCGLHARA
                     DVLIREETGWHLIEIKSSAADPAKPNGAVKKHLPEVAFQTVAFEEAGVPISHSSLLHV
                     NKHYRRNSDVRPEDVLALTDISTFVRKARSGTEARIAAALACLQDGSQVAACDCDRKT
                     RANRCELFAHFHPNIPAQDTIYHITGIHQSMLLPAIDRGIIHLVDWPDDLPLSPKQKR
                     QVELSRTGRQVIQRDRIEALFRTLRYPLYFLDYETFQQPIPLWEGFTPQQQVPFQYSL
                     HVVEEDGAKTHYEHMCTERGENPIPELVLNLRNHVGDTGSVIVWNKAFEESRNREMAA
                     LLPEHAPFLDHINRRMVDLADVVSKGWWVHPEFGGRWSLKAVLPVAAPDLGYDELEIG
                     NGGAASERWTQCMVDDFDTVPEQEREEVIEALRTYCHLDTLAMIRIWEHVLDLITA"
     gene            3772..4722
                     /locus_tag="VGR29_09080"
     CDS             3772..4722
                     /locus_tag="VGR29_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2073779.1"
                     /translation="MVSILSLKCPVCGAPFSPGDDRCGFCGSILVLQTDHPRIDPRAL
                     NKAVIDDQIREYRKDLRSDPYDVTAHYGLGAAYYNLGLTDAAIQSLKDACQLTPENPN
                     IHTQLAVAYRELAKAGDPDAAREKSEHIQYALRLDPQNVEAILLRSDEELERHDYDQA
                     VAEVTRAYGIEPNRVRPKLVETLVAWIRWKDQQGEDTEADWQRLEEINPAVAHEERQR
                     AGKTAAAPLPAPPRADSPEIHRTTLTIWDKARSAFKGFMVGVGIGLISIIALAIIGGL
                     LERDSGPYNVVIALFALSFVIPIVLAIRGWYTAGRKDTSS"
     gene            4719..5234
                     /locus_tag="VGR29_09085"
     CDS             4719..5234
                     /locus_tag="VGR29_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEV2073780.1"
                     /translation="MTQASSNPKIQSLLDNLVQAARERSARGNATAADWRSIAEIDEE
                     RARRELSRYLRTHASGANVEANPYEGNRRSVGTVLGFVLGALGMVVMWLWGVPYLQDA
                     WAGITYGPGFATALTFVSTTLVPLGAWAGSRWDKRFQRSETPPSHTDYTFDEMLQRFM
                     RTQNRSRTRVY"
     gene            complement(5338..5835)
                     /locus_tag="VGR29_09090"
     CDS             complement(5338..5835)
                     /locus_tag="VGR29_09090"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV2073781.1"
                     /translation="MSEKQAMNETIITAEPNSPYLTITREFDAPRERVFQAFVDPALV
                     VQWLGPRGYEMEAVKYDATTGGSYHYIHTGQDGEAHQFRGSFHSVDGPNSIVQTFEYL
                     GYPGPVNLEWMTFEDTGNGRTRLVQHAVAQSVENRDALIQSGMEQGVREGYERLDELL
                     RESED"
     gene            complement(5832..6170)
                     /locus_tag="VGR29_09095"
     CDS             complement(5832..6170)
                     /locus_tag="VGR29_09095"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2073782.1"
                     /translation="MAENQLDRVFSALADPVRRDIVERLSHGEATVNELAEPFSISLQ
                     AVSKHLKVLEGAGLITRSRDAQRRPCRLNTEALDLSAQWIERYQRRKEEQFERLDALL
                     REHPEEEKSS"
BASE COUNT         1369 a         1994 c         1980 g         1250 t
ORIGIN      
        1 cccgtcgcgc gttcgagaag ggagatctct cgcaagagga gtacgagacc ttcatcgagg
       61 ggcagatcaa ggcggtcatc gagcatcagg aagaactcgg tatcgatgtg ctggtccatg
      121 gcgaaccgga acggaacgat atggtgcagt acttcggcga gcaactggag ggctttgcct
      181 ttactcggaa cggctgggtg cagagctatg gctcacgcta tgtccgcccg ccggtgatct
      241 ttggcgatgt ctcgcgtccc gagccgataa cagtccactg ggcgacgtac gcacagtcgc
      301 tgagcgagaa gccggtaaag ggcatgctga ccgggccggt gacgatcctg aactggtcct
      361 tcgtgcggga tgaccaacca cgctcgctca cttgcaagca gatcgcattg gcgatccgtg
      421 atgaggtcgc ggaccttgat gcggccggga tcaggatgat ccagatcgac gaggccgccc
      481 tgcgcgaggg tctcccgcta cgccgcgagg actggcagga gtatctcgac tgggcaatcg
      541 agtgcttccg gattactgcc tcgggtgccc gcccggaaac tcaggtgcac acgcacatgt
      601 gttactcaga gtttggcgac atcttcgggg cgatcagcga tatggatgcg gatgtcatct
      661 ccatcgagaa cgctcgctcc gggctggagt tgcttgagac gttcaaacgc gagggctacg
      721 acaagggagt cggtcccggg gtctatgaca tccacagccc ccgcgtgccc ccggtcgagg
      781 agatcgccgg gaacctgagg gccacgctga ccgtactcga tgccggacag gtgtgggtaa
      841 acccggactg cgggctgaag acccggaagg acccggagac aaccgccgcc ctcaggaaca
      901 tggtggaagc gacgaagcag gttcgcgccg cgctcgtgag ttagtcaccc tcacgcaaaa
      961 cccaacaccg gaaacgcctc cgaggtgact gtcatcccgg aggcgttcct tattgtgctg
     1021 gacacgggcg tcctccagta tgagatggga tgcgtcgccg acctccgggt agttcgctct
     1081 cacgctggag ggtcaatgta ccgatacgcg gttgcatccg ccttgcggga gctttcgtct
     1141 gatatattgg atgaattttc ttggaggtcc acatgctcaa ggtgcagcga aaatcaatct
     1201 cagccaatgc ggccaaacag cgatggggat cgctcatggg tgcagtggaa aaggatggtg
     1261 atcgggttgt cgtggagtcg cacggtaaac ccaaagtcgc ggtggttccc gtacacgatc
     1321 tggagcggct ggaggcgctc gatgagcggg aacatcgcga ggaagcgctg cgcaggctcc
     1381 gtgcgttaga gaagaaagcc actgacagga accaggagtt gactgaggag gatatcgaag
     1441 agctctctgt ccaagtggga cgtgagataa accggagagc ttccgaaaag caacgtgaaa
     1501 tgtcgcgaca tccgcagcag cgatgagggt gagcatcgat accaacgtct ttgtcagcta
     1561 ctacctcgat cagagggctg atcggaaccc ggcccgagtc attcaggcgg cctttgatgg
     1621 ccattttgag ttagtcctga gtgagacact cctgcaagag ctcaccgcga ccatcgaatc
     1681 aaaaccatac ctgcggtcgc gggtcagcga aaacgagctc aacctgttca tccaacgcat
     1741 tcgaggcagc acgagaatca caccggaaat cctcggagaa atcccgagcc taactcgtga
     1801 ttccaccgac gattacatca tcgtgcatag cctgctcgat aatgtcgacg tgctcgtcac
     1861 tggcgacaag aacctgcttg tccttggtcg tatcgacaat ctccatatcg ttaccccagc
     1921 cgcattcatc tttcttctgc agcagggcga ggaatgagac tccggagatt tctccacccc
     1981 ctgctacagc tacaccacga cgagctcccg gtggaattca tcgctggtca gcgcgcaact
     2041 gcccagctgc gactcgagac ggaaatctcc atcaccagcg acagagcgat gggcgatcgc
     2101 ggcatcccaa cctcgttgta cagacggtct atactttccc aggaacgcat tgggacgcgg
     2161 cagtaagcag gtgacaggac acagggtgcg cgacggtacg gtagtggttt cgacatcgga
     2221 ttatctcaat tggctgacat gtcctggctt ttcctgggcg gtcatgcacc agccggaact
     2281 gactcctccc gaggacgcga cctcccgccg caagcacatc gccgaagaca cggtcgagac
     2341 attagccagg actctcttcc cggacggtct cccgatcgac acaggagacg ctgaggagga
     2401 tgcagcccgc acccgggatg cgatgacggc cggcgccgac atcatcttcc aggcgactgt
     2461 catcaccggg tgcggcctcc atgcgcgggc cgatgtgctg atccgcgagg agaccggctg
     2521 gcacctgatc gagatcaaga gctccgccgc cgatcccgca aaacccaatg gtgccgtcaa
     2581 gaaacatctc cccgaggtgg cgttccagac agtggcgttc gaggaagcgg gcgtccctat
     2641 ctcgcacagc tcattgctcc atgtcaacaa gcactaccgg cgcaacagtg atgtgcggcc
     2701 agaggacgtc ctcgcgctga cggatatctc cacattcgtg cggaaggccc gaagtgggac
     2761 ggaggcaagg atcgccgccg ctcttgcctg cctgcaggac ggatcacagg tggcggcctg
     2821 cgactgcgac cgaaagactc gcgccaaccg ctgcgagctc tttgcccact tccaccccaa
     2881 tatcccggca caggacacga tctatcacat cacggggata caccaatcca tgctgctgcc
     2941 ggcgatcgac cgtggaatca tccacctggt cgactggccc gacgacttgc cgctctcgcc
     3001 gaagcaaaag cgccaggtgg agctctcaag gaccggtcgc caggtgatcc agagagatcg
     3061 catcgaagcc ctgtttcgga cactccgcta cccgctctac tttctcgatt acgagacgtt
     3121 tcagcagccg atacccctgt gggaaggttt caccccgcag caacaggtac ctttccagta
     3181 ttccctgcac gtcgtcgagg aagatggtgc caagacccac tatgagcata tgtgtacgga
     3241 gcgtggggag aatccaattc ccgagctggt gctcaatctc cggaaccatg tgggtgacac
     3301 cggcagcgtg attgtctgga acaaggcgtt cgaggagagc cgtaaccggg agatggccgc
     3361 gctgctcccc gagcacgcgc cattcctcga ccacatcaac cggcggatgg tcgacctcgc
     3421 ggacgtcgtc agcaagggat ggtgggtgca cccggaattt ggcggccgct ggagcctgaa
     3481 ggcggtgctg ccggtcgccg cgccggacct tggttacgat gagctggaga tcggcaatgg
     3541 cggcgctgcg tcggagcgct ggacccagtg catggtggac gatttcgata ccgtcccgga
     3601 gcaggagcgc gaggaagtca tcgaggccct ccgcacctac tgccatctgg atacgctggc
     3661 aatgatccgc atctgggaac acgtgctgga cttgattacc gcttgaccca ggttgccggg
     3721 gagggccatg caccccggta tagtcgtcag ggacgttgtg aggtctgttg agtggtttcg
     3781 attctttccc ttaaatgccc ggtgtgtggc gcaccctttt ctcctggtga tgaccggtgc
     3841 ggcttctgtg gctccatcct tgtgctccag accgatcatc cgcgtatcga cccccgcgcc
     3901 ctcaacaagg cggttattga cgaccagatt cgcgaatacc gaaaggacct gcggagcgac
     3961 ccttacgacg tcaccgcaca ctatggattg ggtgcggcct attacaacct tggcctcacc
     4021 gacgccgcca tccagtctct caaggacgcg tgccagctga caccggagaa cccgaatatc
     4081 cacacgcagc tcgccgtggc ctaccgggag ttggccaagg ccggggaccc tgatgccgcc
     4141 agggagaagt cggagcatat ccagtacgca ttgcgactcg acccgcaaaa cgtcgaggcg
     4201 attctgctgc gcagtgacga ggaactggag cgacatgatt acgatcaggc cgtcgccgag
     4261 gtgacacgcg catatggaat cgagccaaat cgggtcaggc caaagctggt ggagacactg
     4321 gtagcctgga tccggtggaa agaccagcag ggtgaagaca cagaagcgga ctggcagcgc
     4381 ctcgaggaaa tcaatccagc cgttgcacat gaagagcggc agcgagcggg caagacggcc
     4441 gccgcaccgt tgccggcacc gcccagggcc gattctccgg aaatccatcg gacgaccctg
     4501 acgatatggg acaaagccag gagcgccttc aagggtttca tggtcggcgt aggcattggg
     4561 ctgatctcca ttattgccct ggctatcatc ggggggctgc tggagcgcga ctcgggtcct
     4621 tacaacgtcg tcatcgcgct gttcgcgctc tccttcgtta taccgatcgt gctggccata
     4681 cgaggttggt acaccgccgg aagaaaagac acatcctcat gacgcaggca tcctccaatc
     4741 caaagatcca atccctgctt gataacctcg tgcaagccgc ccgcgagcgc tcggccaggg
     4801 gaaatgccac cgccgccgac tggcgttcca ttgccgagat cgacgaggag cgggcgcgcc
     4861 gtgagctctc tcgctacctc cggactcacg cttcgggtgc caatgtggag gcgaatccct
     4921 atgaagggaa tcggcgctcg gtggggacgg tcctcggctt cgtcctgggc gcgctgggca
     4981 tggtggtgat gtggttgtgg ggggtgccat acctgcagga tgcgtgggcc gggatcacct
     5041 acggccctgg cttcgccacg gccctgacat tcgtatcgac tacgttggtc ccgcttggcg
     5101 cgtgggcagg atcccgatgg gacaagagat ttcagcggtc ggagactcca ccctcccata
     5161 ccgactacac atttgatgaa atgctccagc gcttcatgcg cacgcagaat cgcagccgga
     5221 cgcgggtcta ctagccgcct ccactagggg acacggacag ggccaccgat cggtggccct
     5281 gtctggcgca tacctttttc ttatccaggg aggatgcgcc acaagggctt ttcctgccta
     5341 gtcttcagat tcgcgcaata gctcatcgag tcgctcgtat ccctcccgga caccctgttc
     5401 cataccgctc tgaatcaatg catcgcggtt ctcaacactt tgggcgacgg catgctgaac
     5461 cagccgcgtc cgtccgttcc cggtatcctc aaaggtcatc cattcgaggt tgaccggtcc
     5521 gggatacccg agatattcga atgtctgcac gatggaattc ggcccgtcca cggaatggaa
     5581 cgacccgcgg aactggtgag cttcaccatc ctggccggtg tggatgtagt gataggagcc
     5641 acctgtggtg gcatcgtact tgaccgcctc catctcgtag ccacggggcc caagccactg
     5701 cacgaccagc gcaggatcga caaacgcctg aaagacccgt tcacgagggg catcgaactc
     5761 ccgagtgatg gtgagatagg gagaattggg ttcggcggtg ataatcgtct cgttcatggc
     5821 ctgcttctca ctcacgatga cttctcctct tccgggtgct cgcgcaacag ggcgtcaagt
     5881 cgctcaaact gctcttcctt gcgccgctgg taacgctcga tccattgggc cgacaggtcg
     5941 agggcttcgg tgttcaatcg gcagggacgt cgttgggcgt ctcggctccg ggtgatcaga
     6001 ccggcgcctt cgagcacctt cagatgcttc gacacggctt gcaagctaat ggagaagggc
     6061 tccgccagct cattcaccgt tgcctcgcca tgggagagcc gctccacgat atcccggcgc
     6121 accgggtccg caagcgcgga gaagactctg tccaactggt tctccgccaa tgcaccacca
     6181 cttcctctat actcaaccaa tcggttgaat acgcaagggg tcaggtttcc gggtttgcaa
     6241 attcctcgcg ccggcataag gaaccgacac cagatccaga gccagtgcat gggtgaactt
     6301 cgaggcaaac agccttccga agcgtacctg gagcccaagc acgaacaggc ttcagcaccc
     6361 ggcgaatatg tcggtccggc cggatcatgc atcgcctgtt gtaatgaagg ctctacggct
     6421 cgcctacgcc tgcgactgcg cttgctccac agctcgttca atggcgctcg ggaatcgcgt
     6481 ttcgaagtcg aggataccga cccattccgg ggtgatgaag acgcgggaca tccgcgggca
     6541 gatcggacgc atgttctcca gccaggcccg cgcctgctcc tcgcccatga ccc
//
LOCUS       DASYWM010000223         4747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_253643, whole genome shotgun sequence.
ACCESSION   DASYWM010000223 DASYWM010000000
VERSION     DASYWM010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253643"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VGR29_09100"
     CDS             complement(<1..819)
                     /locus_tag="VGR29_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV2073783.1"
                     /translation="MAEVIMPKMGDAMEEGTLLKWLKAVGDEVAEGDALAEIETDKVT
                     LELEAQEDGFLTNTLVEEGAVVPIGESVAIIGTQDEVGQSAPAPAAAPEEMQEASAEA
                     EQAPASPAADGATEAAAGQPAEKRGESAQQVDGRKPGERVRASPLVKRLAQEHGIDLS
                     QVAGTGPGGRIINADIQPYVSGEKSAPQAAVPDAPMEAAAPSPSPAPLAAEGKGVKVD
                     MPKIKRTTGKRMSESKLGIPHYYVTSNVDMEAALAFRKQVNVSLGEDGGRVSVND"
     gene            complement(826..1809)
                     /locus_tag="VGR29_09105"
     CDS             complement(826..1809)
                     /locus_tag="VGR29_09105"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV2073784.1"
                     /translation="MPVMTYRDALRSAMAEEMERDESVVLLGEDIGIYGGTHLVTNGL
                     IDQFGPKRVIDTPIAEGGFTGAAIGMAMAGLRPVVEMMTWNFSFLAADQIIQNAAKLR
                     YFSGGQVNVPLVIRGPNGGGVQLSAQHTHSLEGFYGHFPGLKVVSPATPADVKGMMIH
                     AIRDPDPVIFLEAGALYGTKGEVAEGDAEVEFGKARVAREGTDLTLISYGRQVPMLLK
                     VADQLAEDDIDAEVIDIRSIRPFDSDTIVQSVRKTHRAVVVQEQWRYFSVASEVAAVI
                     QEQAFDDLDAPVERVSGAEVPAPYARNLEIAAFPSVDQVVSAARRVLYLEA"
     gene            complement(1889..2938)
                     /locus_tag="VGR29_09110"
     CDS             complement(1889..2938)
                     /locus_tag="VGR29_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV2073785.1"
                     /translation="MKPEQPAADQAQEGQAEVHFDAEALVGFYQKMVEIRLFEDGASR
                     GFRQGKIGGYLHVYSGQEAVGTGFINYLEDGDRTITAYRDHAHVILQGGDPNAVMAEL
                     YGKGTGIVKGKGGSMHLFHVEGGMYGGYGIVGGHIPLGVGMAYGLRYRNEPGIVQLYL
                     GDGSIHNGAFHEAANLAGLWGREGLCPCLFVLENNQYGMGTSVERATAMTDLGRKFES
                     YGIEWEQVDGMDLEAVLGVAERATKYVRETGKPYAVEALSYRLVPHGAADFLEKYRTK
                     EEVRKWREKDPITTLYQRLLEMGVEESRLEEIRAEAKEKADAAVKFAEESPEPDLSEL
                     TTDVYAPDVAVATAG"
     gene            complement(3590..4033)
                     /locus_tag="VGR29_09115"
     CDS             complement(3590..4033)
                     /locus_tag="VGR29_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073786.1"
                     /translation="MAFAKRKFALFGKRVSDGRFFAPEAPGAVRLRHNPGEVPDRSMV
                     PEEGELPVPPPDEGTRRPLTEYGTAQQIMEVYDKNGLDPTDPNRFVQAPPSYEEQRKQ
                     FAGALKTPLRPQPGYALTAGSRLPSEQLAALSAEAQANQPFDPTE"
     gene            complement(4154..4612)
                     /locus_tag="VGR29_09120"
     CDS             complement(4154..4612)
                     /locus_tag="VGR29_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073787.1"
                     /translation="MSIDPPKSDKGIPGPIIVLGFVSLLLLVVVAIFGMLAFGIELPG
                     TMEEESAAIDPADVLVSSGFTVIDMATGGQVATLDVEFVVRNTSDERLENVQVLVQCE
                     DGGYASAITMIPEVAAASEQTIRMQLSGTGEPACREPIIAFSSQRESSTE"
BASE COUNT          880 a         1555 c         1352 g          960 t
ORIGIN      
        1 gtcgttgacc gaaaccctgc cgccgtcctc cccaagcgac acgtttacct gtttgcggaa
       61 cgccagggcc gcctccatgt cgacattcga ggtgacataa tagtgcggaa taccaagctt
      121 cgactcggac atccgcttgc cggtcgttcg cttaatcttc ggcatgtcga ccttgacgcc
      181 cttaccttcc gctgccagtg gggcagggga gggagaagga gcggcagcct ccataggagc
      241 atccggaacc gccgcttgcg gcgctgactt ctcgccggaa acatacggct ggatatccgc
      301 attaattatc cggccaccgg gacccgtgcc tgccacctgg gacaaatcga tcccatgctc
      361 ctgggcaagc cgcttcacca gcggtgaggc acgtacccgc tctccgggct tgcgaccgtc
      421 tacctgctgg gcgctttcac cgcgcttctc agcaggctgc cccgcggctg cctcggttgc
      481 cccatccgcc gcaggggaag cgggcgcctg ctccgcttct gccgaagcct cctgcatttc
      541 ctcaggcgcg gcggccggcg caggagcact ttggccaacc tcgtcctgcg taccgatgat
      601 ggcgacgctc tcaccgatcg gtacgaccgc accttcttcc accagggtat tcgtcaggaa
      661 gccgtcctcc tgggcctcga gctccagcgt gaccttgtcg gtttcgattt ccgccagcgc
      721 atctccctca gcgacctcgt ctccgacagc cttgagccac ttgaggagcg tgccctcttc
      781 catggcatcg cccattttgg gcatgattac ttcggccatg aactcttatg cctccagata
      841 gagcacgcgg cgggcggcac tgaccacctg gtccacgctt gggaatgcgg ctatttcaag
      901 attgcgcgcg tatggagccg gaacttcggc accactcaca cgctcgaccg gtgcatcaag
      961 atcgtcgaac gcctgttcct ggatgaccgc cgcgacttcg gaggcaacgc tgaaatagcg
     1021 ccattgctcc tggacaacga ctgcgcgatg cgtcttgcgg accgactgga cgatggtgtc
     1081 gctatcgaag ggacgaatcg aacggatatc gatcacctct gcatcgatgt cgtcctcggc
     1141 caactggtca gcgaccttca ggagcatcgg gacctgccgg ccgtagctga tgagggtaag
     1201 gtcggtgccc tcgcgagcca cccgcgcctt cccgaactcg acctccgcat cgccttcggc
     1261 tacctcaccc ttggtgccat aaagagcgcc cgcctcaagg aagatcaccg ggtcgggatc
     1321 acggatcgca tgaatcatca tgcccttgac gtcagccggg gtcgccgggg aaaccacctt
     1381 gagtcccggg aagtggccgt agaacccctc aagcgagtgg gtgtgctgcg cagaaagctg
     1441 aacgccaccg ccatttggac cgcggataac cagcggcaca ttgacctgtc caccagagaa
     1501 ataccgcagt ttcgcggcat tctggatgat ttggtcggcg gcgaggaacg agaagttcca
     1561 tgtcatcatc tcgacgaccg gtctcaaccc cgccattgcc atcccgatcg cagcgccggt
     1621 gaagccaccc tcggcgatgg gagtgtcgat cacacgcttc ggcccaaact ggtcgatcag
     1681 cccattggtc acgaggtgag tgccgccgta gatgccgata tcctcaccca acagcacgac
     1741 cgactcgtcg cgctccatct cttccgccat cgcgctgcga agtgcatcgc gataggtcat
     1801 gactggcatc agccgaatcc ctttctcaag tactggggca aaaaggcgag cgtcgctgcc
     1861 cgagcgttac cccatctgca ggagtgtcct aaccagcggt tgctaccgcg acatccggtg
     1921 catacacatc ggtcgtcagc tccgagagat ccggctccgg gctttcctcg gcgaacttca
     1981 ccgccgcatc ggccttttcc ttcgcctctg cccggatctc ctccaagcgt gattcctcaa
     2041 cgcccatctc cagcaatcgc tgataaagcg tcgtgatcgg gtccttctcg cgccacttcc
     2101 gaacctcttc cttggtgcgg tatttctcaa ggaagtcagc ggcgccgtgc ggcaccagcc
     2161 ggtaggagag agcctcgacc gcatagggct tgccggtctc gcgcacatac ttcgtggcgc
     2221 gctccgcaac cccaaggacc gcctccaggt ccatgccgtc gacttgctcc cattcgatcc
     2281 cgtaggattc gaacttcctg ccgagatcgg tcatggcggt ggcgcgctca acactggtgc
     2341 ccatgccgta ctggttattc tccagtacga agaggcaagg gcagagcccc tcgcggcccc
     2401 agaggcccgc caggttggct gcctcgtgga acgcaccatt gtggatggag ccatcgccca
     2461 ggtagagctg cacaatgcct ggttcgttgc ggtagcgaag accgtaggcc ataccgaccc
     2521 ccagcgggat gtggccaccg acgatgccgt accctccgta catccccccc tcgacatgga
     2581 agaggtgcat ggatccgccc ttgcccttga cgatgccggt gcccttgcca tagagctcgg
     2641 ccattaccgc gttgggatcg cccccctgaa ggatgacgtg ggcgtgatcg cgataggcgg
     2701 tgatagtgcg gtcgccatct tcgaggtagt tgatgaagcc ggttcctacc gcctcctgac
     2761 cgctgtacac atggagatag ccgccgattt ttccttgccg gaatccgcgt gacgccccat
     2821 cttcaaacag gcggatctcc accatcttct ggtagaaccc gaccagggct tcagcatcga
     2881 aatgcacctc agcctgtcct tcctgagcct gatcagcagc aggttgctct ggcttcatcg
     2941 cctgctcctg ctgattctct ttactgcggg tggttgccat atggaggcct ctcgaactgc
     3001 tatcgaacac tgcgtcaacc gcactgctca agcgattccg gttacaggaa gcagggatct
     3061 caagatccct cctgccttgc agataccggt cccctgccat gtcacacgcg aaaacggaga
     3121 gtggtaattc tccgaccgac gggctgcggc tgccccgata ttgcagaacg cgggccacag
     3181 catggccccg gcgtaccggg tacgtccgcc gaattgccgg aattgtaccc cacaacgtac
     3241 gccggtagac ctcccgcaga cgcctgctga cgaatgctga catcacaagc accagtacca
     3301 gccggcacca gtggttcgtc atacctggtc cgtctgcgaa cgacgggtgt cctgttggac
     3361 ggacgggcag gggagggagc cgaaggagca aaagacacag caattgcccg gattcgggcg
     3421 tagacgggtc ttgcacttcg ggcattcata gaagaactgg cacgagtcgg tcggcatcgt
     3481 cgcgaccgac tcgtgatggc agtgcgggca tgtgatgggg gatacccact caatcggggc
     3541 catgctgcac ctcggccata aaggcggatg ccatcctcag gagtggcacc tattccgttg
     3601 ggtcaaacgg ctggttcgcc tgcgcctctg cactcagcgc tgccagctgt tcgcttggca
     3661 agcggctgcc ggcggtaagg gcatagcccg gctgggggcg aagcggtgtc ttcagcgcgc
     3721 ccgcgaactg tttgcgctgc tcttcgtagc tcgggggtgc ctggacgaag cggttgggat
     3781 cagtggggtc gaggccgttc ttgtcgtaga cctccatgat ctgctgggcg gttccatatt
     3841 ccgtcagggg tcgtcgcgtt ccctcgtccg gcgggggcac cggaagctcg ccctcctctg
     3901 gcaccatcga acgatccgga acctcccccg gattgtgacg cagcctcact gcaccgggag
     3961 cttcaggggc gaaaaagcgc ccgtcgctca ccctcttgcc gaaaagcgcg aatttgcgtt
     4021 tggcgaaggc catggttgat cccgtctcct gcacaccagc atcgggcgtg cgacatcctt
     4081 tatcctggtt acctgcaacg caagtatcgc gcccggtccg cgctattcat cctctttata
     4141 gtagcagcgg cgtttactca gtgctgctct cccgctgtga ggaaaaggcg atgatcggct
     4201 cccggcaggc cggctcacct gtgccgctca actgcatccg gatcgtctgc tcactggcgg
     4261 cggccacttc ggggatcatc gttatcgccg aggcgtagcc gccatcctca cactgcacaa
     4321 gcacctggac attttccaga cgctcatcgc tggtattgcg cacgacgaac tcgacgtcga
     4381 gcgtggcgac ctgcccgccg gtcgccatat caatgaccgt gaatccggag gagacaagga
     4441 catcggcagg gtctatcgct gctgattctt cttccatggt gccgggaagc tcgataccga
     4501 aggccagcat gccgaagatg gccacgacca cgagcaacag cagggagaca aacccgagga
     4561 cgatgatcgg gcccggtatc cccttgtcac tcttcggcgg atcaatgctc acgaatgtat
     4621 tcctctcgtg cgtctcgtgg ctttctggat gggactatcg cagccattcc tgcctattcc
     4681 tgctaatacc attccattcc agcctgtttc gataaaccct tacaacgatg gtcgatttac
     4741 gatatgc
//
LOCUS       DASYWM010000224         4603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_65672, whole genome shotgun sequence.
ACCESSION   DASYWM010000224 DASYWM010000000
VERSION     DASYWM010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65672"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VGR29_09125"
     CDS             complement(<1..187)
                     /locus_tag="VGR29_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073788.1"
                     /translation="MSAYRLVITERVGTLGDVTDASLRRTALYDRHRALGARIIPFAG
                     WEMPVQYEGIIAEHRAVR"
     gene            complement(196..1638)
                     /gene="purF"
                     /locus_tag="VGR29_09130"
     CDS             complement(196..1638)
                     /gene="purF"
                     /locus_tag="VGR29_09130"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV2073789.1"
                     /translation="MISVQPSSTPPVLADFDDKPHEECGVFGIYAPGEDVARVTFFGL
                     YALQHRGQESAGIAASDGASISLRRNLGLVSQAFDEEDIRSLPGFAAIGHTRYSTAGS
                     TLQCNAGPMYADTDLGPMAIAHNGNLTNAVQLRKELISRGEEFMTTTDSEVLTRMIAV
                     AEGDTVHARIRNAMPRMIGAFSLAILTPEAVYAVRDPMGVRPLCIGKLDTGFVVASES
                     CALMTVGATFVREVEPGEVVRIDTDGVNSLGLGLPHRHAMCLLELIYFARPDSEIMGD
                     RLHMVRQKMGEELAREAPVDADVVVGLPDSATPAAIGYARASGIPYSEALIKNRYIGR
                     TFIQPNQKMRQTQVKLKFNVLPEVVRGKRVVLVDDTIVRGTTSRPIVDLVRQAGAREV
                     HMRVHAPPIMWPCYLGVDLAAREELIAARMSVQEIGRHIGADSIDYLTLDGLFKAIGK
                     GDKRFCTGCMTGHYPVPTFDQIDKMALEHA"
     gene            complement(1673..4015)
                     /gene="purL"
                     /locus_tag="VGR29_09135"
     CDS             complement(1673..4015)
                     /gene="purL"
                     /locus_tag="VGR29_09135"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEV2073790.1"
                     /translation="MISDKLREIEVGSSIGIPRNSSPLDRNEAISGGSYAFHTFTRRI
                     GLTIAEGQWREVALSDSEYARICELLEREPSEVELGMFGAMWSEHCGYKHSRPLLRNL
                     PTDAPWVVQGPGENAGAVDLGNGYAAVFKVESHNHPSAVEPYEGAATGVGGIVRDIFT
                     MGARPIALLDGLRFGPLDQPRNAYLYSHVVAGIGGYGNCLGVPTVGGEVFFHESYNGN
                     PLVNALCVGIAPIDGIVRAAASGVGNMIMLIGAETGRDGLHGATFSSVDDPEASHRGV
                     IQVGNPFLEKQLMEACLELLAQGSVVAMQDLGAAGLTSSIVECASRGEVGVDIDVAMV
                     PRRETGMTAYEVMLSESQERMVLVATPGRVDEITEVLNRWSLSAAVIGTVTDDGLIRV
                     RDGNETCVEVPVRFLIDECPVYPVEAEEPAYLQQTRHFDVDTLSDISAEAIPEKLKTL
                     LGSPNIGTRRSIWEQYDHTILTNTVLGPGQSDAAVLRQKGTSGGIAVSMDCNSRYVYL
                     DPFEGARHAVAESARNVACAGARPLGLTNCLNFGSPERSPANYQLVRAVEGLTDACRA
                     LNIPVVSGNVSLYNETDRQPVFPTPIVACVGVLGNIDHRMSLSWHEGDDIWLIGGGKP
                     SLGGSEYLAYLHNLVSGVPPALDLEAEARMLQVLQALAAGAGDVAAHDVSMGGLAVTI
                     AEMALVTGVGAIIDGAVDGSRTDETWFGESATRVVVALSPVHARVLLSLGEEWETPVI
                     HLGRAQGSALQFPGANSIQLDELMSASESALDVGVEAEMA"
     gene            complement(4032..>4603)
                     /locus_tag="VGR29_09140"
     CDS             complement(4032..>4603)
                     /locus_tag="VGR29_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2073791.1"
                     /translation="ATVLLIDMSRSMFYNGCWDAAKRSALALDTLLRQQFQRDDLELA
                     GFSERAERLSLMQLPSLEWNEYSHGTNLEDGLRLARELLRPYRGKTRQIILITDGEPT
                     AYNEEGTVVFENPPTERTFDATLREVIRCAREDITINAFLLDRTAGMTEFIETVARLN
                     RGRVIHATAGDLGSYLIRDFLSHRTILIG"
BASE COUNT          944 a         1444 c         1295 g          920 t
ORIGIN      
        1 tcctgactgc ccggtgttcc gcaatgatgc cttcatactg aacgggcatc tcccaaccgg
       61 cgaacgggat aatccgggcg ccgagcgccc ggtgccgatc gtacagagcg gtgcgcctaa
      121 gtgatgcatc agtcacgtcg cccaaagtcc ctaccctttc cgtgatgact agacggtatg
      181 ctgacactcc gccagttatg cgtgctcgag ggccattttg tcgatctgat cgaaggtagg
      241 aacgggataa tggccggtca tgcaaccggt gcaaaaccgc ttgtcaccct tgccaatggc
      301 cttgaacaga ccgtcgagtg tgaggtagtc gatgctgtct gccccgatat ggcgaccgat
      361 ttcctgcaca gacattctcg ccgcgatcag ctcctcccgg gccgccaggt caacgccgag
      421 gtagcatggc cacatgatcg gcggtgcatg taccctcata tgcacttccc ttgctccggc
      481 ctggcgcaca agatccacta tcggcctgga agttgtaccc cggacaatcg tgtcatcaac
      541 cagtacgacc cgctttccac ggaccacctc cggcaggacg ttaaacttga gcttgacctg
      601 tgtctgacgc atcttttggt taggctggat gaatgtgcgg ccaatgtagc ggttcttgat
      661 cagggcctca gaatagggta tgccggaggc gcgcgcatac cctattgccg ctggcgtggc
      721 ggaatcgggc aacccgacga ccacgtcagc atcgacaggc gcctcgcgtg ccagctcttc
      781 ccccatcttc tggcgaacca tatgaagacg atcgcccatg atctcgctat ccgggcgcgc
      841 gaaatagatt agctccaaca agcacatggc gtgacgatgt gggaggccga gtccaaggga
      901 attgacgccg tccgtgtcga tacgaacgac ctcgcctggc tccacttccc gaacaaacgt
      961 tgcgccgaca gtcatcagtg cgcaggattc cgaggcaacc acgaacccgg tgtcgagctt
     1021 gccgatgcaa agcggacgca ctcccatcgg gtctcgcacc gcatagaccg cttccggggt
     1081 cagaatcgcg aggctaaacg ctccaatcat gcggggcatc gcattacgaa tgcgggcgtg
     1141 gacggtatcg ccctccgcga cggcgatcat ccgtgtcagg acctcagagt ccgtcgtagt
     1201 catgaactcc tcgccacggc ttatcagctc cttacgaagc tgtaccgcgt tcgtcaggtt
     1261 gccgttatgc gcgatcgcca tcggacctag atcggtatcg gcgtacattg gaccagcgtt
     1321 gcattgaagc gtggatcccg cagtggagta ccgggtgtga ccgatcgctg cgaagccggg
     1381 caaggatcga atgtcttcct cgtcaaaagc ctgcgatacg agtcccaggt ttcggcgcag
     1441 ggaaatgctc gctccatcgc tggcggcaat gccggcgctc tcctggcccc ggtgttggag
     1501 ggcatagagt ccaaagaatg tgacgcgggc gacatcctcg ccgggcgcgt agattccgaa
     1561 caccccacat tcctcatgcg gcttgtcatc aaaatcggcc agtaccggag gagtactgga
     1621 aggttggacc gatatcattg aggagtctcc gatgtgtcct gggttaccgc tctcacgcca
     1681 tttccgcttc aacgccgacg tcgagtgccg attcactcgc tgacatcaac tcgtccaact
     1741 ggatgctgtt tgccccggga aactggagcg ctgagccctg cgcgcgccca aggtggatga
     1801 ccggcgtctc ccactcctct cccaacgata gaagcacgcg ggcgtgcact ggcgagagcg
     1861 ctacgacaac ccgagttgcg ctttcaccga accaggtctc gtcagtccgg cttccgtcaa
     1921 cggcaccatc aattattgcg cccacgccag ttacgagcgc catttcagcg atagtgaccg
     1981 ccaggccacc catcgagacg tcgtgagcag cgacgtcccc cgcacccgct gccagcgctt
     2041 gcaacacctg tagcatcctc gcttcagcct cgaggtccag ggcaggagga actccagaaa
     2101 cgaggttgtg caggtaggca agatattcgc tcccaccaag cgacggtttt ccgccaccga
     2161 tgagccagat atcgtcccct tcatgccatg agagcgacat ccggtgatcg atattgccca
     2221 gcacgcctac acaggcgaca atcggcgtgg gaaaaacagg ctggcggtcc gtctcgttat
     2281 agagactcac attgccgctc acgaccggga tattgagtgc acgacatgca tccgtcaggc
     2341 cttccaccgc tctcacgagt tggtagttgg caggggagcg ttcagggctg ccgaaattga
     2401 ggcaattggt gagacccaac ggccgggcgc cagcgcaggc gacattccgc gcagactccg
     2461 caactgcgtg ccgagcgcct tcgaacgggt caaggtaaac atagcgggag ttgcagtcca
     2521 tcgacacggc aatcccaccc gatgtcccct tctgccgcaa aacggcagcg tccgattgac
     2581 cgggcccgag caccgtattg gtaaggatcg tgtgatcgta ctgctcccaa atactgcgac
     2641 gcgtcccgat gttcggtgat ccaagaagtg tcttcaattt ttcaggaatc gcttctgccg
     2701 aaatgtcgct caatgtgtcg acgtcgaaat gacgggtctg ctgcaggtac gctggctcct
     2761 cggcctccac cggatacacc gggcactcat ctatcaggaa tctcaccggc acctcgacac
     2821 aggtctcgtt gccgtcacgg acccttatca gtccatcatc cgtcacggta ccaatcaccg
     2881 ccgcgctcaa cgaccaccgg ttcaagacct cggtgatttc gtcgacgcgg cccggtgtgg
     2941 cgacgaggac catgcgctcc tgactctcgc tgagcatgac ctcataggcg gtcatgccgg
     3001 tctcccgtcg tggcaccatc gcaacatcta tatcgacgcc aacttctccc cggctggcac
     3061 actcgacgat cgaacttgtc aggcccgctg cgccgaggtc ctgcatggcg acaactgacc
     3121 cctgagccag gagctccagg caagcttcca tcagctgctt ctcaaggaac ggattgccga
     3181 cctggatgac accccggtgt gatgcttccg gatcgtcaac agaagagaag gtagccccat
     3241 gaagtccgtc tcgaccggtc tccgcgccga tcagcatgat catgttgccc accccgctgg
     3301 cggccgcgcg gacgatgccg tcgatggggg cgattcccac acacaacgca ttgactagtg
     3361 gattgccgtt gtaactctca tggaagaaca cttcaccccc gaccgtcggt accccgaggc
     3421 agttcccata gcctccgata ccggcaacga cgtgggaata caggtatgca ttgcgaggct
     3481 ggtccaatgg gccaaaccgc aggccgtcca gaagcgcgat cggcctggcg cccattgtga
     3541 aaatgtcgcg aacgattccg ccaacgccgg tagctgcccc ctcgtatggt tcaactgcgc
     3601 tcggatggtt atgggactcg accttgaata cagccgcgta cccatttccc agatcgactg
     3661 ctccagcgtt ctctcccggg ccctggacaa cccagggcgc gtcggtgggc aaattccgca
     3721 ggagcggtcg cgaatgcttg tatccacagt gctcgctcca catcgcgcca aacattccga
     3781 gctccacctc ggatggttcg cgttcaagca actcgcagat gcgcgcatat tcgctatcgc
     3841 tcagcgcgac ctcacgccac tgtccctcgg cgatagtcaa accgattctc ctcgtaaagg
     3901 tatggaaggc atacgaaccg ccggaaatcg cttcatttcg gtccagcggg gatgagttgc
     3961 gggggatacc gatagaggag ccaacctcga tctccctaag tttatcacta atcaccggtt
     4021 agtggctgct cttatccaat gaggattgtt cgatgcgaga ggaagtcgcg aatcaggtag
     4081 cttccaaggt cgccggccgt cgcgtggatg acgcgtccgc ggtttaggcg ggcgacggtt
     4141 tcgatgaatt ccgtcatccc ggcagtgcgg tcgagcagga atgcgttgat cgtgatgtcc
     4201 tcgcgcgcgc atcgaataac ttcacgaaga gtagcgtcga aggtcctctc agtaggcgga
     4261 ttttcgaaca ccaccgtccc ctcctcgttg tacgccgtag gttctccgtc agtgatcagg
     4321 atgatttgac gggtcttccc tctgtatggt cggaggagct cgcgtgcaag cctcagaccg
     4381 tcttccaggt ttgtgccgtg gctgtactcg ttccactcca gtgaggggag ctgcatgaga
     4441 gagagccgct ccgctcgctc ggaaaacccc gcaagctcca gatcgtctcg ctggaactgc
     4501 tgccgaagaa gggtgtccag cgccagggcg gaacgcttgg cagcatccca gcagccgttg
     4561 tagaacatcg accgactcat atcgatgaga agaacagtcg ccg
//
LOCUS       DASYWM010000225         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_346922, whole genome shotgun sequence.
ACCESSION   DASYWM010000225 DASYWM010000000
VERSION     DASYWM010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346922"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VGR29_09145"
     CDS             <1..577
                     /locus_tag="VGR29_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HEV2073792.1"
                     /translation="EGVLYFIGFIASIWFANWLLTHWGTIRFPGGPWLIPVWPGDLTP
                     SGDPIYAPSGVIAIGLSFTLRDLVQRRLGVGVALVAVVIGAALSAALDPTLALASGVA
                     FLLAESLDLFVYTPLQRRNLVGAVVASNVVGLVVDSIVFLSIAFGSLALLEGQVIGKA
                     WMTLVAIPLIYGIREWDSKRGMMPTGVKVQA"
     gene            complement(580..1554)
                     /locus_tag="VGR29_09150"
     CDS             complement(580..1554)
                     /locus_tag="VGR29_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073793.1"
                     /translation="MSLQTNATVESAGIPPSSLGQQQRGRSSDARLYLRALLHDKVTL
                     AAVIFLLVLLFCALFAPLVAPFDPEAQSLMLRNQPPMTEDPNGGSLPHLLGTDQLGRD
                     LLSRLFYGARISLAVGIAGAAFSGTVGVVLGLIAGYYRGVVDDIIMRLVDTQMGFPSL
                     MTALLVLYVLGPGMWKVILVLAVTRWMVFARVTRSLVLSTREEVFVEAARTVGCTDRR
                     ILFRHILPNLLSPLLVLVTLEVATMILTEASLSFLGLGIQPPQSSWGLMLAQGRAYVT
                     SAWWLVTFPGLIILFTALSLNLVATWARAVSDPVTRWRYLGGSKVAAR"
     gene            complement(1551..2456)
                     /locus_tag="VGR29_09155"
     CDS             complement(1551..2456)
                     /locus_tag="VGR29_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2073794.1"
                     /translation="MIRRFVHSLVILLSLVIVVFFVTHMLGDPARLMLRPEATEEQVQ
                     ALRDTLGLNDPLHVQFGRYMTDLSQGDFGESIWQRVPALPIVLDRIPATLYLAGVTLL
                     IAFPLALVLGIISAVRPRSIADRIVTIVSLGGVSTADFWLGLMLILLFAVQLGWVPTS
                     GYGGLQYVILPALALAMRPIGRISQVVRSAMIDEMSKPYMTTARAKGLRERTVVFSHA
                     LKNGFVPVLTLAGDEASVMLNGAVVIETLFGWPGVGILLIQAIERRDLPLIEASVITI
                     AVMIVTVNLFVDLMYTLLDPRVRYT"
BASE COUNT          554 a          750 c          809 g          488 t
ORIGIN      
        1 cgaaggcgtg ctctatttca tcgggttcat tgcctcgatc tggtttgcga actggctcct
       61 gacccattgg ggaacgatcc gttttccggg cgggccgtgg ctgattccgg tctggcccgg
      121 tgatctcacc ccctcgggtg atccgatcta cgctccatca ggtgtcatcg cgatcgggct
      181 cagcttcacc ctgcgtgacc tcgtacagcg acggcttggt gttggcgtgg cgctagttgc
      241 cgtcgtcatc ggtgcggccc tctcggcggc actcgaccca acgctggcgc ttgcctcggg
      301 tgtcgcgttc ctgctggcgg agtcgctcga cttgttcgtc tacacgccgt tgcaacgtcg
      361 caatctggtt ggcgccgttg tggcgtccaa tgtcgtcggt ttggtggtcg acagcatcgt
      421 tttcctctcg atagcgttcg ggtcactggc gcttctcgaa ggccaggtca tcggcaaggc
      481 atggatgacg cttgtggcga taccccttat ctacggaatc cgcgaatggg acagcaagcg
      541 tggaatgatg ccgactggtg tcaaggtgca ggcttaaggc tagcgggcgg ccaccttcga
      601 tccgccaaga tagcgccagc gagtgaccgg atcgctgacc gcacgggccc aggttgccac
      661 cagattaagg gatagcgcgg tgaacaggat gatcagcccg gggaaggtca cgagccacca
      721 ggcggaggtt acgtatgccc gcccctgggc aagcatgagt ccccatgacg attgcggcgg
      781 ctgaatcccg agaccaagga aactgagcga cgcctcggtg aggatcatgg tcgcgacctc
      841 gagcgtgacc agtaccagaa ggggcgatag gagattcggc aggatatgcc ggaagagaat
      901 cctccggtcc gtgcagccaa ccgttcgtgc cgcttcgacg aacacttctt ctcgcgtcga
      961 cagcaccagg ctgcgggtga cacgggcaaa gaccatccat cgggtgacgg cgaggacgag
     1021 gatgaccttc cacatcccgg gacctagaac gtagagcacg aggagggccg tcatcagtga
     1081 cgggaatccc atctgcgtgt cgaccaagcg catgatgatg tcgtcaacga cgcctcggta
     1141 gtaccccgcg atgagcccca ggaccactcc cacagtcccc gagaaggcgg ctccggcgat
     1201 accgaccgcc agcgaaatgc gagctccgta gaacaagcga gagagtaggt cgcgtccaag
     1261 ctggtcggtg cccaggaggt gggggagcga tcccccgttc gggtcttccg tcattggagg
     1321 ctggttacgg agcatgaggc tttgcgcctc cgggtcaaag ggtgccacca gtggggcaaa
     1381 gagggcgcag aacaggagaa ccaggaggaa tatgacagct gccagcgtta ccttgtcgtg
     1441 aagcagggca cgcaagtaga gtcgtgcatc gctgctgcgg ccacgctgtt gctggccaag
     1501 tgatgaaggt gggatccctg cgctctccac ggtggcattg gtttgcaggc tcatgtgtat
     1561 cgcacccgtg gatcgagtag tgtgtacatg aggtcaacga acaggtttac ggtcacgatc
     1621 atcaccgcaa tggtgatgac cgatgcttcg attaacggca aatcacgtcg ctcgatggcc
     1681 tggatcagca agatgccaac gcccggccaa ccgaagagcg tctcgatcac cactgcgcca
     1741 ttgagcatga ccgacgcctc gtcacctgcc aacgtaagaa cgggaacaaa gccgttcttt
     1801 agtgcatgcg aaaagacgac ggtacgctcc ctcaggccct ttgcacgcgc cgtcgtcata
     1861 tatggcttcg acatttcgtc gatcatcgcg ctgcgaacaa cctggctgat tcggcctatt
     1921 ggacgcatcg caagtgccaa agcgggcaag atgacatatt gcaggccgcc atatcctgat
     1981 gtaggtaccc agccgagttg caccgcgaag agcaggatca gcatgaggcc gagccagaag
     2041 tcggccgtgg agactccgcc gagcgagacg atggtcacga ttctgtcggc aatcgaacgc
     2101 ggccgcaccg ccgagatgat ccccagaaca agcgccagag ggaaggcgat caggagcgtg
     2161 acacctgcca gatagagcgt agccggaata cgatcgagca cgattggcag ggccggtacg
     2221 cgttgccaga tggattcacc gaaatcgccc tgggagagat cggtcatgta gcgaccaaac
     2281 tggacgtgca ggggatcatt caggccaagg gtatcccgta acgcctgaac ctgctcttca
     2341 gtcgcttccg gccggagcat gaggcgcgcc ggatcgccca gcatgtgcgt cacaaagaaa
     2401 acgacgatga ccaggctcaa caggatcacg agtgaatgta caaatcgtcg gatcatgtat
     2461 cgagcgatga caacatcctc ctgagtgaat cccactgctg accggtgtgg tccccgggat
     2521 gatgagtctc atccagaaaa ccacaccggt gctgaggtgc gccttctcgc gactacaaga
     2581 gcttcatttc cttcaggagg a
//
LOCUS       DASYWM010000226         4749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_628702, whole genome shotgun sequence.
ACCESSION   DASYWM010000226 DASYWM010000000
VERSION     DASYWM010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628702"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VGR29_09160"
     CDS             complement(<1..816)
                     /locus_tag="VGR29_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2073795.1"
                     /translation="MTRSGIGSSTGSPPTEKNSFSRRRLLGAGGALLLSNQSRRHSAG
                     AQTPVATPLPPARLRVAVSALPGSFDPAAAQTLEHIWLNTLVYDSLARWNAEGEVIPA
                     LGLNWFITAYDRIIDLSLRPNAFFFGGKSLTADDVRWTLERLRNSEGTDDAWRLEHVW
                     RIESLDPRTVRVILNAPDASLVSSLASPVLAVLPDATNLSQVQAGTGPYAINFHSSEL
                     VTFNRNPLFWEMGRPNFQSLRVRRVRDDTERSTTLVTGAVDLIPGAPLLDIPTL"
     gene            complement(813..1994)
                     /locus_tag="VGR29_09165"
     CDS             complement(813..1994)
                     /locus_tag="VGR29_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV2073796.1"
                     /translation="MAETMTTSALMELDKGKQIPLYAKRDIVLVRGEGVYLYDSEGNQ
                     YLDAMSNYGVAVLGHAHPRFTAAVTGQLQTLTTCHQSFGNDARAELLAELDTIAPEGL
                     TRSFLSNSGAEAIEAALKFAQVTTGRQRIVAAKRGYHGRTLGALSATAEKKYRAPFEP
                     LPLPSEHIAYNDGDALEAAVNSETAAIILEPVQGEGGIHPADDAFLQRAREIATANGA
                     LLIMDEIQSGFRTGAPFATTVAGVSPDIVCTAKALANGLPIGLTMVTEAVSDQMPGGA
                     HGSTFGGNPLVARGAVETLRIMRDDDLYARAERVGAYTVRAIEALSHPRIRAVRGRGL
                     MMGVELKQKATPVLRGLQENGVLALLAGNLVIRFLPPMTWEEAHADELVDSLARVLGP
                     A"
     gene            complement(2064..2705)
                     /locus_tag="VGR29_09170"
     CDS             complement(2064..2705)
                     /locus_tag="VGR29_09170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2073797.1"
                     /translation="MSSQRIQAIVFDLGETLENETRFWSSVARYAGVPELTLHGVLGG
                     LIERRENHRSIFNVMQIESVDPKGVGYEIELKDLYPDVVPVLKQLCHAGYRLGVTGNQ
                     PEGAIEQFQALGLPLDFYASSTNWGVSKPDPVFFQRLIEELGLEPGEIMYVGDRLDND
                     VIPAQELGMHAVFIRRGPWGYLHAAWPEAEQVRYHIDALTELPKLLETINGDE"
     gene            complement(2702..3526)
                     /locus_tag="VGR29_09175"
     CDS             complement(2702..3526)
                     /locus_tag="VGR29_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HEV2073798.1"
                     /translation="MLVVKIGGGAGIGADVYTRFAQDIASIEEPVLVVHGGNAEFSQL
                     CDALGMPPRMITSKSGRVSRYTDAETMDAMLMAYCGKVNKHAVADFRAEGLNAVGLSA
                     IDGGIAYGQRKAVIRGTEADGKMKVLRDDHAGTVETFDPSLIRTLMGAGYVVLISPPG
                     LSEGGVPINFDGDKLALGLAEAMGASGLLFFSDTPGLLQDRYDESTLIREIDASDPEQ
                     ALAAAAGRMVVKVEQAIKAVEKGVGKVIFADARIERPVTRALAGEGTVVRTSDSKQ"
     gene            complement(3535..3834)
                     /locus_tag="VGR29_09180"
     CDS             complement(3535..3834)
                     /locus_tag="VGR29_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV2073799.1"
                     /translation="MQKQFYVYILASYTQRLYIGVTSDLERRVWQHKNKAFEGHSAKY
                     NIDRLVYVELFGHADDAIAREKQPKNWSRIKKVNLITRDNPNWRDLSIELFGWPP"
     gene            complement(4203..>4749)
                     /locus_tag="VGR29_09185"
     CDS             complement(4203..>4749)
                     /locus_tag="VGR29_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEV2073800.1"
                     /translation="DMPVVTEAKTGSSGSGGSPSPASHHSERSGVVRSFKATGHRHTA
                     EIIQELTFDGAKPSMAFSATAIEAVRGILTTSHVFLKDDLQDKDLWKIFRGAYKEEPF
                     MRLVKENAGVHRYPEPKLLSGSNWCDVGFEKDPHSKRVVVMAATDNLMKGASGQAVQS
                     FNIRMGFPEETAIDFPGLHPI"
BASE COUNT          939 a         1522 c         1367 g          921 t
ORIGIN      
        1 cagggtcggg atatccagga gcggtgcgcc ggggatgaga tcgacggcac ccgtcaccag
       61 ggtcgtggac cgttccgtat cgtcccgaac ccttcggaca cggagggact ggaagttcgg
      121 cctgcccatc tcccagaaga ggggattacg gttaaaggtc acaagctccg acgagtggaa
      181 gttgatggcg tagggaccgg ttccagcctg cacctgtgac agatttgtgg catcagggag
      241 cacggccaga accggcgacg ccagggaact tacgagagat gcgtccggcg cattgaggat
      301 aactcgtacc gtgcgcggat caagcgattc gatgcgccac acgtgctcca gccgccaggc
      361 gtcgtcggta ccctcactgt tccgcagacg ctccaacgtc catcgcacgt catccgccgt
      421 cagggacttg ccaccgaaga agaaggcatt cggccggagg gagagatcga taatccgatc
      481 gtaggcggtg atgaaccagt tcaaccccag tgcaggaatg acctcgccct cggcgttcca
      541 ccgtgccagc gaatcgtaga cgagcgtgtt gagccagata tgctccaatg tctgggccgc
      601 cgccggatca aacgatccag gcaaggcact gacagccaca cgaagccttg ccggagggag
      661 cggagtagcc accggtgtct gggcgccggc ggaatggcgg cgagactggt ttgacagcag
      721 caatgctccc cctgccccga gcaaccggcg gcgtgagaac gagttttttt cagttggggg
      781 agagcctgtc gacgagccga tgcctgatcg ggtcatgcgg gcccaagcac gcgcgccagc
      841 gaatcgacca actcatccgc atgcgcttct tcccacgtca tcggcggcag gaaccggatc
      901 accaggttcc cggcaagcag ggccagcacc ccgttctcct gcaggccgcg cagcaccggg
      961 gtcgccttct gcttcagctc cacgcccatc atcaggccac ggccacgcac ggcgcgaatc
     1021 ctgggatggg agagcgcctc gatcgcccgg accgtgtagg cccccacccg ctctgcccgc
     1081 gcatagagat catcgtcccg catgattcgt aaggtctcga cagcgccacg tgccaccagt
     1141 ggattaccac caaacgttga gccgtgcgca cctcccggca tctggtcgct aaccgcctca
     1201 gtcaccatcg tcaggccgat gggcagaccg ttcgccaggg ccttggcggt gcagacgatg
     1261 tctggcgaga cgccagcaac ggttgtggcg aagggcgcgc ctgtgcggaa gccggactgg
     1321 atctcgtcca taattagcag ggcaccgttg gcggtcgcga tctcccgtgc gcgctgcagg
     1381 aatgcgtcgt ccgcggggtg gattccacct tctccctgaa ccggctccag aataattgcc
     1441 gccgtctcgc tgttcaccgc tgcctccaac gcgtcaccat cgttgtacgc gatgtgctcg
     1501 gacggcaggg gcaggggttc gaagggcgcg cggtacttct tctcggcggt cgcggacagc
     1561 gcgccgagcg tccggccatg atagccgcgc ttcgccgcca caatccgctg gcgacccgtc
     1621 gtgacctggg caaacttcag cgcagcctcg atcgcctccg caccggaatt gctcagaaac
     1681 gagcgtgtca gcccttccgg ggcgatggtg tccaactctg ccagcagttc ggcacgggcg
     1741 tcattaccaa agctctggtg acatgtggtc agtgtctgca actgcccggt gaccgcggcg
     1801 gtgaaccgcg gatgcgcatg accgaggact gccacgccat aattgctcat ggcatcaaga
     1861 tattgattac cttcgctgtc atagaggtag accccctcgc ctcgcacaag cacgatatcg
     1921 cgcttggcgt acagcggaat ttgcttgccc ttgtcgagct ccatcagggc gctggtagtc
     1981 atcgtctctg ccattggatc ggttccttgt cctacctgcc gccccgggag gatcgtcagg
     2041 cctttatgtg ccattccgtc gattcactcg tcgccgttga tcgtctcgag gagtttagga
     2101 agctcggtga gtgcatcaat gtgataacgc acctgctccg cctcgggcca cgctgcatgg
     2161 agataacccc agggaccgcg gcgtatgaag acggcatgca ttcccagctc ctgggcagga
     2221 ataacatcgt tgtccagacg gtcaccgacg tacataatct cccctggctc cagtccaagc
     2281 tcctcgatca ggcgctggaa aaagaccgga tcaggcttgg aaacgcccca attcgtcgag
     2341 gaagcgtaga agtccagcgg caaccccagc gcctggaact gctcgatcgc accttccggc
     2401 tggttgccag tcactccaag ccggtagccg gcatggcaaa gctgcttgag cacgggtacg
     2461 acgtccgggt aaagatcctt cagctcaatc tcgtagccaa cacccttcgg atccacggac
     2521 tcaatctgca ttacgttgaa aatgctgcga tggttctcgc gtcgctcgat gaggccgccc
     2581 aggacaccat gcagggtcaa ctcggggact cccgcataac gggcgaccga cgaccagaag
     2641 cgcgtctcgt tttccagcgt ttctcccaaa tcgaagacaa tggcctggat gcgttgggag
     2701 ctcattgttt gctatctgat gtgcgcacga cagtgccttc tcctgcgagc gctcgagtga
     2761 caggacgctc gatccgggca tccgcgaaga tcaccttccc aacgcccttc tctaccgcct
     2821 tgatcgcctg ctcgaccttc acaaccattc gtcccgccgc tgcggcaagc gcctgctccg
     2881 gatcggaagc gtcgatctca cggatcaacg tgctctcatc ataacggtcc tgcaacaaac
     2941 cgggcgtatc ggagaagaag agcaggccag acgcgcccat cgcctcggca aggccaaggg
     3001 caagcttgtc gccgtcaaag ttgatcggca ccccgccttc ggaaagcccc ggtggcgaga
     3061 tcagcaccac atacccggca cccatcagcg tccggatcag ggagggatcg aatgtttcga
     3121 ccgtgcccgc gtggtcgtca cgcaggacct tcatcttccc atcagcctcc gtccccctga
     3181 tcaccgcctt gcgttgcccg taggcgatgc cgccatcgat cgcgctgagc ccgacggcat
     3241 tgaggccctc cgcgcgaaaa tcagcgacgg catgcttgtt caccttgcca cagtacgcca
     3301 tcagcatcgc gtccatcgtc tcggcatcgg tatagcgact gacgcgcccg cttttggagg
     3361 tgatcatccg cggcggcatg ccgagagcgt cgcagagctg gctgaactcg gcattcccac
     3421 cgtggacgac caagacgggc tcctcgattg aggcgatgtc ctgcgcgaac cgtgtgtaga
     3481 cgtccgcacc gatgccggct cctccgccga tcttgacgac aagcatgtgt gctcctatgg
     3541 cggccaaccg aacaactcga tagagaggtc cctccaattg ggattatctc tcgtgatcag
     3601 attgaccttc ttgatacgtg accagttctt aggttgcttt tcccgagcga tcgcatcgtc
     3661 cgcatgaccg aagagctcaa cgtacacaag ccgatcgatg ttgtacttcg cactgtggcc
     3721 ttcgaatgcc ttgttcttgt gctgccacac gcggcgttcg agatcgcttg tgacaccgat
     3781 gtacaggcgt tgggtatagc tggcgaggat atacacgtag aactgctttt gcatttaccc
     3841 ggttctctac cataacccag taattccgac gcatcgctcc gccaccaagc ccgtcatccc
     3901 gagccgatga gccgcgcgca gcggggcgaa tcgtgtcgag ggatctttgc ttctgcctga
     3961 cggctagacc gcctgccaca aagattcctc gacttcgctc agaatgacga ttaagttggc
     4021 ctgagcacct caaaaacgcc ctctcgaaga accgcccctc gtcatcctga acggatgaaa
     4081 cgaccccggc cagtcgaatc ccatcgaagg ctcttgacgc tacccagcca agaccttcgc
     4141 caagagcaaa gattcctcgg cttcgctcgg aatgacgagg tattacagtc ggggatatct
     4201 cgctaaatcg ggtgcaggcc tgggaagtcg attgccgttt cttccgggaa gcccatccgg
     4261 atattgaacg actgcaccgc ctggcccgag gcacctttca tcaggttgtc cgtcgcagcc
     4321 atgacgacca cacgtttgga gtgcgggtcc ttctcgaacc caacatcgca ccagttcgag
     4381 ccactcagca gcttcggttc cggatagcga tggacacccg cgttctcctt caccagccgc
     4441 atgaagggtt cttccttgta cgctccgcgg aagatcttcc agaggtcctt gtcctgcagg
     4501 tcgtccttca ggaagacatg cgatgtcgtg agaattccgc gcacggcctc gattgcggtg
     4561 gctgagaagg ccatggacgg cttcgcccca tcgaaggtca actcctggat gatctcggcc
     4621 gtatgtcgat gaccagtcgc tttgaacgag cggacgacac cactgcgctc ggaatggtga
     4681 gatgcgggac tcggagagcc gccagaaccc gaagatccgg tctttgcttc ggtcaccacc
     4741 ggcatatcg
//
LOCUS       DASYWM010000227         9630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_272342, whole genome shotgun sequence.
ACCESSION   DASYWM010000227 DASYWM010000000
VERSION     DASYWM010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272342"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VGR29_09190"
     CDS             complement(<1..314)
                     /locus_tag="VGR29_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073801.1"
                     /translation="MTYRASRRQFIVGVLGISAAPATLPHGASAQIATPSNSNTNSAP
                     EFVVSIGSCGENDLAFERAEFGIVGIYDGDWFEQPGFPHLLDNLAASPQAFHGVRYFG
                     AFT"
     gene            533..988
                     /locus_tag="VGR29_09195"
     CDS             533..988
                     /locus_tag="VGR29_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073802.1"
                     /translation="MSTHHPRTSFTHEEIIDHAKGNGAVLAMALVARAREQGEAPETV
                     ARWLGRILAPGWEQLRGQGARIAAENAALNTVSLGASLQDLSGDERHSEATVADWPGE
                     AMLGMFRLSQDDADVMFAVFEPIAEHIGLSYRWQRQGDSVTMTFEQGGA"
     gene            1174..2184
                     /locus_tag="VGR29_09200"
     CDS             1174..2184
                     /locus_tag="VGR29_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2073803.1"
                     /translation="MPLIRAEHLVKEYRRPKQHSGSFSGVRTLFTRESVVTRAVEDIS
                     FTIDEGEVVGYLGPNGAGKSTTIKMLAGILVPTSGSCEVAGIVPWENRSANAMNIGVV
                     FGQRSQLWWDLPLDESLHLIGKMYRMEPERFRRQREMLVDLLDMKPFLQTPVRQLSLG
                     QRMRGDLAAAVLYEPPVLFLDEPTVGLDVIAKERMRSFVEQINRDNRTTVLLTTHDLD
                     DVERLCKRIIIIDEGRVLFDGRAADLKRRYAPHRLLDVVVAGDATERLETVANTLPQG
                     VQSVSRDGMRATFEIDPEIIRVADVIADVSSRVPVIDLGVREPPLEGIIREIYEAREV
                     TR"
     gene            2181..2996
                     /locus_tag="VGR29_09205"
     CDS             2181..2996
                     /locus_tag="VGR29_09205"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2073804.1"
                     /translation="MIAPVSWRLHPGLIACTMAAIRRTWAYRTRFVLWVVRMIAQLYL
                     LRMIWMALYAGQDMVEGIPANMMIVYLTVAMLQEFLIQPLIVFEIEQRIMRGTVASDI
                     VRPVGFMHQMLAYDFGGILGRAPVLLVAIPLSMVVGSLKLPLTPAAAAGYAVSLILAY
                     LISLMIWLVVGMGGFWMTSTHGLRFLLSTIQSFLAGAVVPLWFLPDILRAVLQLLPFQ
                     GMAFLPLSIFVGQATGIDILVALGIQLFWVIVLALMAALVWRRAQWKLVVQGG"
     gene            3083..3886
                     /locus_tag="VGR29_09210"
     CDS             3083..3886
                     /locus_tag="VGR29_09210"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2073805.1"
                     /translation="MSLTHTLSICWSLIRASIRGQMQYRASFLMEVASGLAYQSMGFV
                     FIWAVLSSFQSLGGWSLREIALLYGMRLTAHGLYMLCFSNVVRIDDFVREGGYDRFLV
                     RPIPPLLQLMFSSIRVTVVGDVLGGIVILMAALTQVDIAWSAANVALLVLALIGGALI
                     DGAFQIGPAALTFRFLESWTLRIIFDDIFSRFGSYPTSILDRPIRSVLTFIVPMAFMA
                     YLPAAALLGRETYLPLWMGWLSPLVGVIVFGLALWLFVRASRGYQSSGH"
     gene            complement(4056..4610)
                     /locus_tag="VGR29_09215"
     CDS             complement(4056..4610)
                     /locus_tag="VGR29_09215"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2073806.1"
                     /translation="MITIKMVAHMDAGDRRLWTEDQDLRPLSELGRRQTARMAEVLSN
                     GPVDGLYSSPALRCRQTLEPLAARFGLPIVTIPELRETNGFLPPPGWDGLPPDMAPAM
                     GGAYAAGRASSALRQIMIGLPEGRVIACSHGDVVPALIAFLISANGLSVPPTYVRRGG
                     WYTIRADEANVSAEHHDVLLDFPI"
     gene            4844..4969
                     /locus_tag="VGR29_09220"
     CDS             4844..4969
                     /locus_tag="VGR29_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073807.1"
                     /translation="MARTRVLRRLHDTMVLAQRLCSGPVMTMMDEFHKGAYATLE"
     gene            5082..5663
                     /locus_tag="VGR29_09225"
     CDS             5082..5663
                     /locus_tag="VGR29_09225"
                     /inference="COORDINATES: protein motif:HMM:NF020022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2073808.1"
                     /translation="MTPPSRTQRPPLIIVNGPPASGKSTLGKEIAAELGIPLLSKDAI
                     KEEMYDSLGKIERKISRRLGETSMRLMYTVARKILDAGLGVVIEANFYHGISEKDLSK
                     LIAVSDAVMVHCTAPEEILKQRYIERAQSGERHPVHDDANRVDELGKDLDDGIYDPLN
                     LGIPLIQVDSSDGFDPSVAEIVSRLQDGPVRAA"
     gene            complement(5669..6049)
                     /locus_tag="VGR29_09230"
     CDS             complement(5669..6049)
                     /locus_tag="VGR29_09230"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2073809.1"
                     /translation="MDQPRMQLSATVLDSRDPQALGDFYARLLWWTVVTNEPDWVMVQ
                     PPAGRPGLSFQREEKYVPPVWPGEAGEQLMMMHLDILVDDLETAVAWAIETGARQADF
                     QPQDEVRVMHDPAGHPFCLFTDTA"
     gene            complement(6240..6611)
                     /locus_tag="VGR29_09235"
     CDS             complement(6240..6611)
                     /locus_tag="VGR29_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV2073810.1"
                     /translation="MRIRESARLVLLNDDNRIFLFEHNSPVTMGRKEPHILRYWVAPG
                     GGVEEGETWEEAARREMWEETGIDDVELGPCIWSREGEVQYSKGIVSREPYYLVRCGN
                     REISNARQRASSLPTASMVVA"
     gene            complement(6648..7232)
                     /locus_tag="VGR29_09240"
     CDS             complement(6648..7232)
                     /locus_tag="VGR29_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073811.1"
                     /translation="MIVEFLRQEFASTDRYMEKISACLDAERVSPGIILNADISNPNE
                     NAQRRRVFARYRGYGTGSPSYLTGFPDHGVSGQWATLTPQEILDVRHIRYCYWTALSA
                     GTRSPVIAARRIRQGIEVYGVSNQRFLQLASLVAQGVAMPPLILVSVPVPIAFVVLEG
                     HTRLTAYALAADHLAPETRVMIGIAPAITRWDEY"
     gene            complement(7305..9179)
                     /locus_tag="VGR29_09245"
     CDS             complement(7305..9179)
                     /locus_tag="VGR29_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV2073812.1"
                     /translation="MANDHTPEGPAPDSTPLEPEQSRRKARKSSDSSSVTPQQRPHYL
                     TRKRPSQSTKASSAQNEGFRPPRRAQERYRTYGEGHRDRRTLGPSASPALAGALAAEF
                     RQAEREQQLVQKRPGNRLRRATRQKWFWPAVGVPGIALLVALIVLAPVVYSAFFAYQD
                     VQVDSVERIQSAFVPQLNRAGTPELVAATSNKSGSTWTGEERITILLLGVDKSDDGPS
                     RTDTLILVNIDPVAKTANMLSIPRDLKTVIPGYGVDKINAAFALGEFNQVQGGGAGLT
                     IRTIEANLGIPINAFVQIDFNGFINMVDTVGGITVDVPYPIKDDTYPADNYQYQRIYF
                     PAGWQQMDGEKALQYARTRHQDGDSRRSARQQQVILSLRDQAIDLDLIPQLPTLIGQF
                     GDSVRTDISIGDAVKLARLGTEIPRGSITQTSLLPALWEEQVPDGPYYLNADWDAVGD
                     VLSGFAGTEISPPGAALVNPDYGAPILIENGTNNEGLAGRIGDILEANGFWNIEVVTA
                     EDAGSHEKTMIQDRENNLGTSSLITQLIGVGADTITLDDGATDVGDGTGPTDNGNYAI
                     VITLGNDAPDPAGDEWNLEDYQRESGNDGAAIEEDPFADDGALEEESSQEFPADDTGN
                     "
BASE COUNT         1716 a         2823 c         3053 g         2038 t
ORIGIN      
        1 gtgaaggcac caaagtaccg gaccccatga aacgcctgtg gagacgccgc caaattgtcc
       61 agcagatgtg ggaagcccgg ttgctcgaac cagtcgccat cgtagatgcc aacgatgcca
      121 aactccgcac gctcaaatgc aagatcgttc tcaccgcatg acccgatcga cacaacgaac
      181 tcaggggcgg aatttgtatt ggaattggag ggagtagcga tctgggccga tgccccatgt
      241 ggcagggtgg ccggggctgc gctgatcccc agaacgccca cgatgaactg gcgccgggat
      301 gctcggtacg tcatgtcgtg ctcatgtcca ttgatgaaaa accggattat ccggttccac
      361 ccagcctcgc cggtagcctg tcatgctcgc cgaatatggc tttcgcgtga gcatggatag
      421 taatctcgtc gctgccgcct gcaaagggcc gattccctag cttaacgatg taccatgatg
      481 tcgggtctct accgtcgaat atcgtcatca tcccatacaa gggagcagca ctatgtccac
      541 gcatcatcct cgaaccagtt tcacccacga ggagattatc gaccatgcca agggcaacgg
      601 agcggtgctg gccatggcgc tggttgcccg tgcacgtgag cagggggaag caccggagac
      661 ggtagctcgc tggctcggga ggatccttgc tcccggatgg gagcagttgc ggggtcaggg
      721 cgcacgcatc gcggcagaga acgctgcgct gaacaccgtc tcactcggcg catccctgca
      781 agacctttca ggagatgaac gtcacagcga ggctacggtg gcagattggc cgggagaggc
      841 gatgctcggc atgttcaggt tgagccagga tgatgccgat gtcatgttcg ccgtcttcga
      901 gcctatcgcg gaacacattg gcctcagtta ccgctggcag cgccagggag attcagtgac
      961 catgaccttc gaacagggcg gagcctgatc actactgcag cagcaggatg catctgcggg
     1021 atcgcccgct acatatccgt gcgtgtggtc cctgtgggct gatcttgttg gtcggtaagg
     1081 agcaatccgg cactggattg aagaagccgt tcccctgccc tgccctcgtg ccggagtggc
     1141 tcttcatttc cctgtcgtca tcggagccaa gtgatgcccc tgatccgagc cgagcatctt
     1201 gtcaaggaat accggcgacc caagcagcac tccggaagct tctccggcgt tcgtacactc
     1261 tttacacgcg aatccgtagt gacgcgggct gttgaggata tctccttcac catcgatgag
     1321 ggtgaggtcg tcggctatct cgggccgaat ggcgccggga agtcgacgac gatcaagatg
     1381 cttgcaggta tcctcgttcc gaccagtgga tcctgcgagg tcgcgggaat cgtgccgtgg
     1441 gagaatcgct ccgccaatgc gatgaacatc ggcgtcgtgt tcggccagcg gagccagctc
     1501 tggtgggatc tgccactgga cgaatccctt catctcatcg gcaagatgta ccggatggag
     1561 ccggagcggt tccgccgtca gcgggagatg ctggtcgatc tgttggatat gaagccattc
     1621 ctgcagacgc cggtgcggca gctcagcctc ggccagcgga tgcgcggtga cctcgcggcg
     1681 gcggtcctct atgagccacc ggtgctcttt ctcgatgagc cgacggttgg gctggacgtg
     1741 atcgccaagg agcggatgcg ctccttcgtt gagcagatca accgtgacaa tcgcaccacc
     1801 gtgctgctca ccacccatga cctggacgat gtcgagcgcc tctgcaagcg gatcatcatc
     1861 atcgacgagg gtcgcgttct cttcgacggc cgtgcggccg acctgaagcg gcgttatgca
     1921 cctcaccggt tgctggatgt cgtggttgcc ggcgatgcga cggaacgcct cgagaccgtc
     1981 gcgaataccc ttccccaagg ggtccaatct gtctcacgcg acggcatgag ggcaacgttc
     2041 gagatcgatc cggagattat tcgggtcgca gatgtcatcg ccgatgtctc gtcccgggtg
     2101 ccggtgatcg atcttggcgt gcgtgagccc ccgctggagg ggatcatccg cgagatctac
     2161 gaagcgcggg aggtcactcg atgatcgcgc cggtctcctg gcgcctgcat cctgggctga
     2221 tcgcctgcac gatggcggcg atccggcgta cctgggccta tcgcacccgg tttgtcctct
     2281 gggtcgtgcg gatgatcgcg cagctctacc tgctgcggat gatctggatg gcgctgtacg
     2341 ccggtcagga tatggtcgag ggcatccccg ccaacatgat gatcgtctac ctcaccgtag
     2401 caatgctgca ggagttcctg atccagcccc ttatcgtctt tgagattgag cagcggatca
     2461 tgcgcggtac ggttgcgtcc gacatcgtgc ggccggttgg ctttatgcac cagatgcttg
     2521 cctatgactt cggtggcatt ctcggccggg cgccagtgct gctggtcgcg attcccctct
     2581 cgatggttgt cgggtcgctc aagttgccac tgacaccggc agcggcggca gggtacgcgg
     2641 tcagcctgat ccttgcctac ctcatctcgc tgatgatctg gctcgtggtt ggcatgggcg
     2701 gtttctggat gacgagcacg catggcttgc gttttctgct atcgacaatc cagagctttc
     2761 tggcgggtgc cgttgtgcca ctctggttcc tcccggacat cctgcgagcg gtactccagc
     2821 tgctgccatt ccagggaatg gcctttctcc cgctctcgat cttcgttggg caagcgacgg
     2881 gcatcgacat cctcgtcgcg cttggtatcc agctcttctg ggtcattgtg ctggccctga
     2941 tggccgcgtt ggtttggcga cgggcgcaat ggaagctcgt cgtgcaggga gggtgatccg
     3001 gagtggccgc ttgggacaca tttgcgcaag ccctgaaatg ccatgccact gaagtggttc
     3061 aagggaggaa caggcggcat tcatgtcatt gacacacacg ctctcgattt gctggagcct
     3121 catccgcgcc tctatccgcg gtcagatgca ataccgtgcc agctttctga tggaggtggc
     3181 gtccggtctg gcctatcaat caatggggtt cgtctttatc tgggccgtcc tcagcagctt
     3241 ccagtcactt ggcggatgga gcctgcgcga gattgcgttg ctttatggca tgcgcctgac
     3301 cgcgcacggg ctctatatgc tctgctttag caacgtcgtc cgcattgacg attttgtgcg
     3361 cgagggcggc tacgatcggt ttctggtgcg gccgatccct cccctgctcc agctcatgtt
     3421 ctcctcaata cgagtcacgg tcgtcggtga tgttctcggt gggatcgtga ttctcatggc
     3481 ggcactgaca caggttgaca ttgcctggtc cgctgcgaat gtggcgctgc tcgtccttgc
     3541 cttgatcggt ggggcgctga tcgatggtgc cttccagatc ggtcccgcgg cgctgacgtt
     3601 ccggttcctg gagtcatgga ccctgcgaat catcttcgat gatatcttct cgcgcttcgg
     3661 gagctacccc acctcgattc tggaccggcc cattcgcagc gtgctgacgt ttatcgtgcc
     3721 gatggcgttc atggcctatc tccctgcggc ggcactgctg ggtcgggaaa cctatctccc
     3781 gctgtggatg ggttggctct cgccactcgt gggcgtgatc gtgtttgggt tggcactgtg
     3841 gctcttcgtg cgcgcctcac gggggtatca gagctccggg cattagagcc agttatggac
     3901 ttggtcgcgt cagcgccacg atggggaagg cggtccggtt gcaacctgca ctgcctcggt
     3961 caaggtgccg atctggccgc gcttctggac gttggcgggg gtcacgggca gcgccattct
     4021 gcgccgcctc tatcaggagg aggactgacg caccactaga tggggaaatc caggagcacg
     4081 tcgtggtgct cggcgctcac gttcgcctcg tcggctctga tggtgtacca gccgccgcgg
     4141 cggacgtagg ttggcggcac cgacagaccg ttggcgctga tcaggaatgc gatcaaggcc
     4201 gggacgacgt caccgtgcga gcaggcgatc acccgcccct ccggcagacc aatcatgatc
     4261 tggcgtaggg ccgaagaggc ccgacccgcg gcgtaggcgc cccccatggc cggggccatg
     4321 tcaggcggta ggccgtccca gcccggaggt ggcaggaagc cgttggtctc ccgcagttcc
     4381 ggaattgtca caatcggcag cccgaatcgc gcggcaagtg gctccagggt ctgccggcag
     4441 cggagggccg ggctgctgta gagcccatcg accggaccat ttgagagaac ctcggccatc
     4501 cgcgccgtct gccgccgtcc gagctcactc agcggccgga ggtcctgatc ctcggtccag
     4561 agacggcgat ccccggcgtc catgtgcgcg accatcttaa tcgtgatcat cgtcctcgag
     4621 ctccttacga ccgggcgatc gtcgcgcgat ccgcaggtgc gcgtcagaaa gtctggagcc
     4681 cattatgggc tttgcgcgac gacggagagc aatcagtgga gcatcaggca ggcgaaggcg
     4741 atacacgttg catggccatt ccacaatcgg agaagatgac gacaagcacc ttcccttctg
     4801 gtgacatcgt ctcgattcag acatgaatca ggagaacgca acgatggcca ggacacgagt
     4861 cctacggcgg ctgcatgaca cgatggtgct ggcgcagcgt ctctgctcgg gcccggtgat
     4921 gacgatgatg gacgagttcc acaagggcgc ctacgcgacg ctggagtaac gtggaatcgc
     4981 ggtgtgggtg ataatcaccg ggattctggc gcagtttctg cgtgcaggaa gcgaccgtgc
     5041 gaggcacgac ctgacgttgc aacccatgcc gaaggagccg tatgacacca ccgtctcgta
     5101 ctcaacgccc gccgctcatc atcgtcaacg gcccacctgc ttccggaaaa tcgaccctgg
     5161 ggaaggagat cgcagccgaa ctggggattc ccctgctctc caaggacgcg atcaaggagg
     5221 agatgtacga cagcctcggc aagatcgagc gaaagatctc gcggcgcctt ggcgagacat
     5281 cgatgcggct gatgtatacg gttgccagga agatcctcga tgcaggccta ggtgtcgtca
     5341 tcgaggcgaa cttctaccat ggcatctccg agaaggatct ctcgaagttg atcgccgtgt
     5401 cagatgcggt gatggtccat tgcacagcgc cggaagagat tttgaagcag cgctacatcg
     5461 agcgggcgca atccggcgaa cgacatccgg tgcacgacga tgccaaccgg gtcgacgagc
     5521 tgggcaagga cctggacgat ggaatctacg atcctctcaa cctggggatc ccgttgatcc
     5581 aggtggattc cagcgatggc ttcgaccctt ccgtagcaga gattgtcagc cgcctgcagg
     5641 atggaccggt ccgggcggcc tgacgacctc aggcggtatc ggtgaagaga cagaacgggt
     5701 gccctgcggg atcgtgcatg acccgcactt catcctgtgg ctggaaatcg gcctggcggg
     5761 ctcccgtctc aatcgcccag gcgacagcag tctcgaggtc gtcgacgagg atgtcgaggt
     5821 gcatcatcat tagttgctca cccgcttccc cgggccagac cggggggacg tacttctctt
     5881 cacgttggaa cgagagcccc ggacgtcctg ccggtggttg caccatgacc cagtcaggct
     5941 cgttcgtgac gaccgtccac cacaggagcc tggcgtagaa atcaccgagg gcttgggggt
     6001 ctcgcgaatc gagcacggtg gctgagagct gcatccgggg ttggtccatg taattcctcc
     6061 ttgatccggg gcaaggcggg tccaggatag tgtccctgca tactccgtgc ccggtgtacg
     6121 aggattgatg tcattccagc aaccgaattg gctgcgtcgg aatctgccct tcgatcaggg
     6181 gtacgagcag atcggcgaga ccgaccgggt agaccacctc tgccgtcact cggagatcgt
     6241 caagcgacca ccatcgatgc cgttggtaga ctcgacgctc gctgtcgagc gttgctgatc
     6301 tcacggttgc cgcatcggac aaggtaatag ggttcgcggc taacgatgcc tttgctgtac
     6361 tggacctcgc cttcgcggga ccagatgcac gggccgagct cgacatcgtc gataccggtc
     6421 tcctcccaca tctcacgacg tgcggcctct tcccaggtct caccctcctc taccccacca
     6481 ccaggtgcca cccagtaacg caggatatgc ggctcttttc ggcccatcgt cactgggctg
     6541 ttgtgctcga agaggaagat ccgattgtcg tcattgagga gcaccaggcg agcactttcc
     6601 cgaattctca ttatccctct cctccccttc acgttccctc gcgcatatca atattcgtcc
     6661 caccgggtga tggcgggggc aatgccgatc atcacccggg tctctggcgc caggtgatct
     6721 gcggcgaggg cgtaggcggt gagacgggta tgcccttcaa ggacgacgaa ggcaatcgga
     6781 accgggacgc tgacgaggat caaggggggc atggcaacac cttgggcgac cagcgaggca
     6841 agctggagga aacgctggtt gctgacgccg tagacctcga tcccttgccg gattcgccgg
     6901 gcggcgatca cgggagaacg cgtgccggcg gagagtgcgg tccagtagca atagcgaata
     6961 tggcgaacgt cgagaatttc ctggggcgtc aaggtcgccc attgcccact cactccgtga
     7021 tccgggaagc cggtcaggta cgatgggctg cctgttccat aaccacggta gcgggcgaag
     7081 acgcgacgac gttgtgcgtt ctcattcggg ttcgaaatgt ctgcgttgag gatgataccg
     7141 ggcgataccc gctcagcgtc caggcacgct gagatctttt ccatatacct gtcggtcgac
     7201 gcgaattcct gccggaggaa ctctacgatc atttccgatt ccgacgacac tcctgttatg
     7261 cgcattgcag ggcagtcttt gctgcgggat tgagatgcgc cctcctagtt cccggtgtca
     7321 tcggcgggga attcctgaga gctctcctcc tcgagagcac cgtcgtcggc aaaggggtct
     7381 tcctcaatgg cggcgccgtc gttgccggac tctcgctggt agtcctcaag gttccactcg
     7441 tcaccggcag ggtcaggagc gtcattaccc agggtgatga cgatggcgta gttcccgttg
     7501 tccgtcgggc cagtgccatc gccaacgtcg gtcgcaccgt cgtcgagcgt gatggtgtcg
     7561 gctcccacgc cgataagctg cgtgatcagg gaggaggtgc cgaggttgtt ctcgcgatcc
     7621 tggatcatcg ttttctcatg gctgcctgca tcttcggcgg tcaccacctc gatgttccag
     7681 aagccattcg cttctaggat atccccgata cgtccggcga gcccttcgtt gttggtgccg
     7741 ttctcaatca ggatgggcgc tccatagtcg ggattgacaa gcgctgcgcc cggcgggctg
     7801 atctccgtgc ctgcaaagcc ggagagtacg tctcccacgg cgtcccagtc cgcgttcagg
     7861 tagtagggac cgtcgggcac ctgttcctcc cagagcgccg gaagcaggga cgtctgggta
     7921 atcgagccgc gtggaatctc tgtgcccagc cgtgcaagct tgacggcatc accgattgag
     7981 atatcggttc ggaccgagtc gccgaactgg ccgatgaggg tgggcagctg aggaatcagg
     8041 tcgaggtcga tggcctggtc acgcaggctg aggatgactt gttgctgccg ggcagaacgg
     8101 cgggagtcac cgtcctgatg ccgggtccgc gcgtattgca gcgctttctc gccgtccatc
     8161 tgttgccatc cagccgggaa atagatccgc tggtactgat agttgtccgc cgggtaggtg
     8221 tcgtccttga tcggataggg cacatccacg gtaataccgc cgacggtgtc caccatgttg
     8281 ataaagccat tgaagtcgat ctggacgaag gcgttgatgg ggataccgag gttcgcctcg
     8341 atcgtgcgta tggtgagtcc cgctccccca ccctgtacct ggttgaactc gccgagggcg
     8401 aaggcggcat tgatcttgtc gacgccatag ccagggatga cggtcttcag gtcacgcgga
     8461 atcgaaagca tgtttgcggt cttcgcgacc ggatcaatgt tgaccagaat caaggtgtcg
     8521 gtgcgtgagg gaccgtcgtc tgatttgtcg acaccgagca gcaggatggt gatccgctcc
     8581 tctccggtcc aggtggagcc agatttgtta gaggtggcag caaccagttc cggggtaccg
     8641 gcccggttga gctgcggcac gaacgcggat tggatgcgct caaccgagtc gacctggacg
     8701 tcctggtagg caaagaacgc cgagtagacg accggggcga ggacgatgag cgcaacgagc
     8761 agcgcgatcc ccggtacgcc taccgccggc cagaaccact tttgccttgt ggctctccgg
     8821 agccggtttc cgggtcgctt ctggacgagt tgttgctccc gttccgcctg ccgaaattcg
     8881 gcggccaagg cgccagcgag ggccggcgag gcggatggcc cgagggtgcg tcgatctcgg
     8941 tggccttcgc cgtaggtacg gtaccgttcc tgtgctcgtc gcggcggccg gaatccctca
     9001 ttttgcgcgc tggaagcttt ggtggactga gaaggacgtt ttcgggtcag atagtgcggt
     9061 cgctgctgcg gcgtgactga cgaactgtct gatgacttcc tcgccttgcg ccgggactgc
     9121 tctggttcga gtggcgtgga gtcgggcgcg ggtccctcgg gagtgtgatc gtttgccatg
     9181 gaatccatag gtggaggacg gagccctgcg gaggggtcgg ttatgcgttt gcctggtgcc
     9241 attggcgggc ttgggtctgt tggtttcctg agcaaagtct accatgtccg cgtgtctatc
     9301 tgatcattat tatgtcaacc gatggatcat gcgggatgga cctcggtacc ctcggctttc
     9361 ggtgtgtcat gcgataatgg gcaactgatg tgcccgctcg aattgcggcc actcgcgttg
     9421 tacgtccggt tgtgggagat ggtgaacctc catgatattg tccgaccgcg atattgtccg
     9481 tgctatggat gagggccgga tcaggattga tccctctccg gatctcgaga aacaactggg
     9541 ttctgtctcc gtagattttc ggctgggcac gaccttcatg gtcttcgagc acagccgttt
     9601 cagctacatc gatccgcgtc atccgcagtc
//
LOCUS       DASYWM010000228        12897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_328791, whole genome shotgun sequence.
ACCESSION   DASYWM010000228 DASYWM010000000
VERSION     DASYWM010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12897
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328791"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VGR29_09250"
     CDS             complement(<1..1193)
                     /locus_tag="VGR29_09250"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEV2073813.1"
                     /translation="MSQQLHLTTRFEPRDERTFRHVPFQVPEGIDQVHISITYNNRIG
                     SSPMVTGGNTLDIGLFDEEGIESGGPGLRGWSGSERTEITIDSAWATPPYRAGSIGSG
                     TWNLLFGAYKVDQHGLDVSVTVSFDTGIEPPIVPPWPDLADLHRSSLPPAAEPGWYRG
                     DLHMHTVYSDGDSYPHEVAAIAYAIGLHFYGITDHNRARSPIDLVPQGNGWPVLVPGV
                     EVTTYAGHFNVWGTDGWYDFRNPTAAGIQAAVDAARAEGGLVSLNHPKPYGPPWEFPE
                     VSGFDCIEPWNGWWGRLNNLSTAFWADALHQSTPNSWPVGIGGSDTHKNQQRGQAANP
                     LSQATMGFPTLWIQTDETLSPQSIIEAIRGGHCFISESPAGPQLYVRREGDEMVVRIV
                     GGEGDA"
     gene            complement(1198..2124)
                     /locus_tag="VGR29_09255"
     CDS             complement(1198..2124)
                     /locus_tag="VGR29_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2073814.1"
                     /translation="MNDASQLEVPLGDKVLDRDTSGSAQREGGSDRLFQYGVIAFLII
                     LMMMMIVPFWNVVAVAFSGPIASMTRGIILWPREFSAEGFRIVWVNLNLQQAFINSVI
                     VTTVGTVLHVALSCFAAYVLIQRNLPGKTIMLVFILATMAIPGELIMIPLYIVNKQLG
                     LLNTLWALIFSGMISGFSILLLRNFFAGVPYDLAESAKIDGASDFRIFRSIYLPLSQA
                     GIATIALFELVSRWNQFLPVILYINDSSKYTLQVALNALVTENLGTSGTSMVTPNVRM
                     AGIVIAILPLVILYPFMQKYFVKGITLGSVKE"
     gene            complement(2126..3016)
                     /locus_tag="VGR29_09260"
     CDS             complement(2126..3016)
                     /locus_tag="VGR29_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2073815.1"
                     /translation="MLRHTTRTMRSHAWLYVMILPVIAYFLIFVYYPLYQGAASSFQE
                     FKLLGTAEWIGTENYLEVWKDGTFWQVVKNTLLIGGGILVFGFFPPIMVALALNEAAT
                     LLFKKLAQTTVYLPHLFSWVVIGGMWIFLLSPGGGLVNELLALINVGPVRFLTNTTWA
                     RATMIGLPIWKDMGYAAIIYLASITSINPSLYEAARIDGASRFQQMYQITLPLLKPTM
                     KVVFMLNILGLLRIFDQIFVMRNPVVQGGIDVIMTYVYDMGIQRYELGFASAISVMVL
                     LTTLLLTAIVWRVTRFGRDD"
     gene            complement(3187..4695)
                     /locus_tag="VGR29_09265"
     CDS             complement(3187..4695)
                     /locus_tag="VGR29_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2073816.1"
                     /translation="MSRKRPFSSSLSRRSLVQRGSALGAGAAVGKVALGSAQTPAGSP
                     AASPVPLTEVDQDFLAAQEAVIAEIQEMGEGKTLHILSAVVGGKTPEEDQLFEEEIKR
                     LTGIQVELVHPTAEYDEKMLADLAAGVEYDLIYMNKNRMDVLVDQEVLTDLTEQINNS
                     AILSNPTVIPPEEWELIKYEGDTFYSVFNKFEGARMLTVRQDWLDQLGLEAPRTLDEI
                     YDVMVAFRDQDPAGNGEGVTLGLSTAGTYDIQPFMSSEGIRPGYVEVDGKRTIPYATE
                     AAIPVYEWLAKLYSEGLYDPSFATAETADMRNLFMTDKVGFVTYWDTWVGLFNALVEQ
                     ENPGSDFQAKGLAAAEGPEGVIISRGQPSVWAIPVNAPNAELAFKFLEWWHTFPGIIL
                     GSLGILDHDYTVEDETYELTEVGAEHAMDHGVPTPYNTNWNNPVGELPGLAEAQEISV
                     EYGYLAQLGPQWDAEVVPILDENIIKAILGDISAADAVAAMQERLTSEGLID"
     gene            5147..6511
                     /locus_tag="VGR29_09270"
     CDS             5147..6511
                     /locus_tag="VGR29_09270"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEV2073817.1"
                     /translation="MTEGVLREPGSSEGNRLTIGVPREAVPGETRVGLIPDTVGRLVA
                     SNVDVLVQSGAGEGSSISDDEYVTAGATVVPDAGSVYERADLIVKVQAPVREGVAGDE
                     LASLRPGQLLVSFLAPLVNHDLVRALADQGVTALAVDAVPRITRAQSMDALSAMSTIA
                     GYKAVLLAADHLPKFFPLLMTAAGTIAPAKVLVIGAGVAGLQAIATARRLGAVVEAYD
                     ARPVVKEQVESLGARFVEIDTGSRDAEAAGGYARAATEVELRLQQEGLNTHVAKSDVV
                     ITTALVPGRPAPRLVPASAVISMRTGSVIVDLAGEAGGNCELSVPGETVVREGVTILA
                     PLNMPSTMPVHASQMYSRVIQNFLGLLIKDGEVNLDLNDAIIKGMCITHGGDVIQEQT
                     RQLMNLGTIEAPVPRNPAQPEPHVEQDSAPAEDTDESESEAESVVMGEAAVEGGDTRE
                     GRPV"
     gene            6575..6892
                     /locus_tag="VGR29_09275"
     CDS             6575..6892
                     /locus_tag="VGR29_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEV2073818.1"
                     /translation="MDSDFMLGIYILTLAIFLGAVLIMRVPSTLHTPLMSATNMISGI
                     VLIGAMLVVGSVDSWWLQVLGFLAIAFSAANVVGGYAVTDRMLKMFKQRAPAAGTSGK
                     GER"
     gene            6894..8303
                     /locus_tag="VGR29_09280"
     CDS             6894..8303
                     /locus_tag="VGR29_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEV2073819.1"
                     /translation="MIDNWRDAFIDLAYFISAVLLIISLNQLGSPKTARVGNQLAVGG
                     LLLAIVATFFNRDIAGGDYWVILLGIAVGGAGGYVMAQRVQMTAMPQMIAIFNGMGGA
                     TSALIAVSEFLGKSDLGRGTVLSIALGCIIGSIALTGSLVAFAKLQGLVPSRTALPGN
                     RQLITAGLLGGMALLGAITVILQDGALAQTVLILTFLLALAAGVLLVMPIGGADMPVV
                     IAILNAFSGVAAVLSGFVVNNQLMIIGGTLVGASGVFLSLLMAKAMNRSIGNVIFGAF
                     GATPSAGGAGTGGGESLPVREVTPDDAAVSLAYADRVIVVPGYGLAVAQAQHSVQELA
                     SLLQERGVDVRYAIHPVAGRMPGHMNVLLAEANVPYDDLYEMERINGDFAKTDVVLVI
                     GANDVTNPAARDDPNSPIYGMPVLRVDEAERVIVLKRGMSTGFAGVENALFHNPKTSM
                     LFGDAKASIGKLIAEVRAA"
     gene            8660..10051
                     /locus_tag="VGR29_09285"
     CDS             8660..10051
                     /locus_tag="VGR29_09285"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2073820.1"
                     /translation="MVIKSQESRVQDALARDGSNINGVMVEQFVSVGDAVTPTLDVNG
                     RPHVVVIGAGFGGLSTVQALRNVDVDVTLVDRRNHHLFVPLLYQVATAQLSADHIAQP
                     IRRILKKQDNAKVVYDEATDIDVDGKSVVLASGKTLTYDFLVIAVGAKDSYFGHDEWA
                     KHAPGLKSLEDAERIRQQILLAFERAEKERDPVKRKALLTFVIVGGGPTGVEMAGAIA
                     EIRNHTLQREFSNFNPRDARVVLLEGMPRVFPMFKEKLSARAQKDLEKLGVEVRTGSL
                     VTDIDEQGVVIGGEERIETETIIWAAGIQAASLTSSLNDVTQLDRSKRVAVTRELTVP
                     GHPEIFVIGDAAIIKSAEDKPWGAVAPVAIQSGKHAAKNIQRFMRGEAFTPFVYHDQG
                     NMATIGRNHGVADIYNMQITGFAAWLLWALVHIMQLVTFRNRVAVAIQWAIDYFGYNR
                     NARVILDHSGDTK"
     gene            complement(10207..10788)
                     /locus_tag="VGR29_09290"
     CDS             complement(10207..10788)
                     /locus_tag="VGR29_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2073821.1"
                     /translation="MSRHNEKRDESEFNAVIPFPPRWVASNIALPDGISDERNDLPGY
                     VVRMLHAEGATQRKVFTSWEARSTSPGTTHVLVMDDNLEILDILGQLLGDEAYDVTLS
                     SSPLGLEEIQDLQPDIIMLDATFNRLTTGPDLMDILREETRTKGIPLISTTILPHIAQ
                     DLADAGYTALLKPFDLDDVLDAVTVAIDQQRRI"
     gene            10975..11424
                     /locus_tag="VGR29_09295"
     CDS             10975..11424
                     /locus_tag="VGR29_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2073822.1"
                     /translation="MLGQFIRERRVELGLTQEQLAERVGDNVRQAEISRLERDRVSLP
                     RRARMERLAKALEVSLGTLLLRTGWMDEEQSIRDALVHMPAAIRPPAPYLEPVIGETY
                     EPNRSGEEIILLNAALSRAREVARRTEDVLRESTMTVDHARRTRRQP"
     gene            11549..12537
                     /locus_tag="VGR29_09300"
                     /pseudo
     CDS             11549..12537
                     /locus_tag="VGR29_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
BASE COUNT         2601 a         3619 c         4071 g         2606 t
ORIGIN      
        1 gcgtctccct ctcctccaac aatgcggacg accatctcgt cgccttcacg tcgcacatag
       61 agctgcggtc ccgcgggtga ttcactgatg aagcaatggc caccgcggat tgcctcgatg
      121 atgctctgcg gcgagagcgt ctcatcggtc tggatccaga gcgtggggaa gcccatggtt
      181 gcctggctaa gggggttcgc tgcctggcca cgttgctggt tcttgtgcgt atcgcttccg
      241 ccaatcccca cgggccatga gttgggggtg ctctggtgta gggcatcagc ccagaaggcg
      301 gtggagagat tgttgaggcg cccccaccag ccgttccagg gctcaatgca gtcgaagcca
      361 ctcacctccg ggaactccca gggtgggcca tagggtttcg ggtggttcag ggagacaagg
      421 ccgccctcgg cgcgggctgc atcaacggcc gcctggatgc ctgctgcggt cggattccga
      481 aagtcgtacc agccgtcagt gccccagaca ttgaagtgac ctgcatatgt tgtgacctcg
      541 acgccgggca cgaggactgg ccagccgttt ccctggggca cgagatcgat gggggaacgg
      601 gcgcggttgt gatccgtgat accgtagaag tggagtccga tggcataggc gattgcggcc
      661 acctcgtgtg gatagctgtc accatcggag tagacggtgt gcatgtgcag gtcaccccga
      721 taccaccccg gctcggcagc aggtggcagg gacgaccggt gcagatcagc gaggtccggc
      781 cagggcggaa cgatcggcgg ttcgatgccg gtatcgaagg agacagtgac gctcacatcc
      841 aagccatgct ggtcgacctt gtatgcgccg aagaggaggt tccaggtgcc gctcccgatg
      901 cttcccgctc tgtagggcgg cgttgcccag gcgctgtcga tcgtgatctc cgtgcgctcg
      961 ctgccgctcc agccacggag gccaggacct ccgctttcga taccctcttc gtcgaagagc
     1021 ccgatatcca gcgtgttacc cccggtcacc atcgggctgg agccgatccg gttgttgtag
     1081 gtaatggaaa tgtggacctg gtcgatgcct tctggtacct gaaaaggcac atggcggaat
     1141 gtccgctcgt cacgcggctc gaatcgggtg gtcagatgga gctgctggga caacgaatta
     1201 ctccttgacc gatccaagtg tgatgccctt gacaaagtat ttctgcatga aggggtagag
     1261 gataaccagg ggcaggatcg cgatgacgat gcccgccata cgtacgttag gcgtgaccat
     1321 cgacgtacct gaggttccaa ggttctccgt caccagcgcg ttgagcgcca cctgcaacgt
     1381 gtacttgctg ctgtcgttga tgtagaggat gacggggagg aactgattcc agcggctaac
     1441 aagctcgaag agcgcgatcg tcgcgatccc ggcttgtgag agtggcaggt agatgctccg
     1501 gaagatgcgg aaatcgctcg ctccatcgat cttcgctgat tccgccaggt catagggaac
     1561 gccagcgaag aagttgcgca ggaggaggat gctgaagccc gagatcatgc cggagaagat
     1621 tagcgcccac agcgtgttca gcagcccaag ctgcttgttg acgatgtaga gcgggatcat
     1681 gatcagctcg cccgggatgg ccattgttgc gaggatgaag accagcatga tggttttgcc
     1741 gggaagattc ctctggatca ggacgtaggc ggcaaagcag ctcagcgcga cgtggaggac
     1801 ggtgccgacc gtggtcacga tgacgctgtt gatgaatgcc tgctgcaggt tgaggttgac
     1861 ccaaacgatc ctgaacccct ccgcgctgaa ctcccgtggc cagaggatga tgccgcgggt
     1921 cattgatgcg atgggtccgg aaaaggcgac ggcaaccacg ttccagaatg gaacgatcat
     1981 catcatcatc aggatgatga ggaacgcgat cacgccgtac tggaagagcc tgtcgctgcc
     2041 gccctcacgc tgcgcggacc cgctcgtgtc ccgatcgagg accttgtcgc cgagaggtac
     2101 ttccagctgc gaagcgtcat tcatctcaat cgtccctccc aaacctggtg acccgccaaa
     2161 cgatggccgt aagcagcaac gttgtcagca gcaccatgac cgagatggcg ctggcgaagc
     2221 cgagttcgta acgctggata cccatgtcgt agacgtaggt catgatcacg tcgatgccgc
     2281 cttggacaac gggattacgc atgacaaaaa tctgatcgaa gatccgcaac aacccaagga
     2341 tattcaacat gaagaccact ttcatggtcg gtttcagcag gggcagcgta atctggtaca
     2401 tctgctggaa acggctggcg ccgtcgatcc tggctgcctc atagagcgag ggattgatgc
     2461 tggtgataga tgccagatag atgatcgccg cgtaccccat gtccttccag atcggcagac
     2521 ctatcatcgt cgcccgggcc caggtggtat tggtcaggaa ccgtactggt ccgacgttga
     2581 tgagggcgag aagttcgttg actaaccctc cgccgggcga gagcaggaag atccacatgc
     2641 cgccgatcac gacccaggag aagaggtgag gcagatagac ggtcgtctgg gcgagcttct
     2701 tgaacagcag agtggcggcc tcattcagcg caagtgccac catgatgggt ggaaagaacc
     2761 cgaagacgag aataccgcca ccgatcaaca gcgtgttctt gacaacttgc cagaacgtgc
     2821 cgtctttcca gacctcgaga tagttctcgg tgccgatcca ttccgcggtg ccgagcagct
     2881 tgaactcctg aaagctcgac gctgctccct gatagagcgg gtaatagaca aagatcaaaa
     2941 aatacgcgat gaccggaagg atcatcacgt agagccaggc atggcttctc attgtgcggg
     3001 tcgtgtgccg tagcatccgc aggtccttgc ataggtggca ttcgtgtgcc tgagacgaga
     3061 ggcggtgccc gagtcggaat ccgtccatcg tacaccggtg cccctccggt tggaatcacc
     3121 agaaggggca cctgcgctga tgcggcttgg cgcgtcgtga tctcacgaca cgccgggcgg
     3181 atgaatctag tcgatcaggc cctcgctggt aagtcgctcc tgcatcgccg caacagcgtc
     3241 ggcagctgag atatcgccga gaatcgcctt gatgatgttc tcatccagga tcggaacgac
     3301 ctcggcgtcc cactgtggac ctagctgggc aagatagccg tactccacgg agatctcctg
     3361 cgcttcggca aggccgggaa gctcgccgac cggattgttc cagttggtgt tgtacggagt
     3421 cggaacgcca tgatccatgg catgctccgc gccaacctca gtcaactcgt aggtttcgtc
     3481 ttcgacggtg tagtcgtgat cgaggatacc gagggagcca aggatgatgc ccgggaaggt
     3541 atgccaccat tcaaggaact tgaacgcgag ttccgcgttt ggcgcgttca ccgggatagc
     3601 ccagacgctc ggctggccac gggagataat gacgccttcc ggtccctcag cggcggcaag
     3661 acccttggcc tggaaatccg atcccgggtt ttcctgttcc acgagcgcgt tgaacagacc
     3721 aacccacgta tcccagtagg tgacgaagcc gaccttgtcg gtcatgaaga gattacgcat
     3781 gtcggcggtc tctgcggtcg cgaaactggg atcgtagagg ccctcggaat acagcttagc
     3841 gagccactca tagaccggga tcgccgcctc ggtggcgtag ggaatggtcc gcttaccatc
     3901 gacctcaaca tagcctggac ggataccttc gctgctcatg aagggctgga tatcgtaggt
     3961 gcccgcggtc gagagaccga gtgtgactcc ctcaccgttg ccagctgggt cctggtcacg
     4021 gaaagcaacc atgacgtcgt agatctcgtc gagggtcctc ggtgcctcga gtcccaactg
     4081 atccagccaa tcctggcgga ccgtcagcat acgggcgccc tcgaatttgt tgaagaccga
     4141 gtagaacgtg tctccctcgt acttgatcaa ctcccactct tcgggaggaa tgaccgttgg
     4201 gttgctgagg atggcggagt tgttgatctg ctcggtgagg tcagtgagca cctcctggtc
     4261 aaccagaacg tccatccggt tcttgttcat atagatgagg tcgtattcca cgccagcggc
     4321 aaggtcggcc agcatctttt catcgtattc cgcggtcggg tgaaccagct cgacctgaat
     4381 accggtgagg cgcttgatct cctcttcgaa gagctgatcc tcctctgggg tcttgccgcc
     4441 gacaacggcg ctgaggatgt ggagggtctt gccctcgccc atctcctgga tctcggcaat
     4501 aaccgcttcc tgggcagcga ggaaatcctg atcgacttct gtaagtggaa ccggtgacgc
     4561 cgcgggggag ccggccggcg tttgggcact accgagggca accttaccaa ccgctgcgcc
     4621 ggcaccaaga gccgatccac gttggacgag cgaacgtcgc gaaagtgatg aggaaaacgg
     4681 acgcttcctt gacatcaggt gatgcttcct ttcgatagag cttgatccat ccagctggcg
     4741 acagcgtccc tggcatattc cgtttcggtt cctcaaccct ggcgggccgc aggaaaactg
     4801 gcaggaatat atctcgtggg cactccccag atggtcaaga aatcgtcgaa cggtacaagt
     4861 gattgattgt cgccatggcg tcattgtacc ggtaacgctg ggttttgaca tggcattatc
     4921 ttggttgttc tcccctgata gagaggttcc cgttgagtcg catcaccgac ccctcgatga
     4981 acgcctaagg cacacatgat cgtggtccta aaagagactg gacaaatacc gagatacgcc
     5041 tgaacatgaa agcttcaact cgggccgcga tgtcggtccc acgccaaata ccatgacgac
     5101 gacaccgtag agtgagttgg actcaaaggg aagggaacag gattgtatga ctgaaggtgt
     5161 attgcgggag cctggatcat cggaaggcaa ccgcctcact attggcgtac ccagagaggc
     5221 agttcccggt gagacgcgtg tcggcttaat ccctgacacc gtgggcaggc ttgtcgcgag
     5281 caatgtcgat gtgctggttc agtcaggcgc tggtgaaggg tcctccatca gcgacgatga
     5341 gtacgtgacg gcgggtgcaa ctgttgttcc cgacgccgga tctgtttacg agcgcgctga
     5401 tctcatcgtt aaggtccagg caccggtccg agagggagtg gcgggagacg agctggcgtc
     5461 gctccgaccg ggccagttac ttgtcagctt ccttgcgccg ctggtgaacc atgatctcgt
     5521 ccgcgcgctt gcggaccagg gggtaaccgc gctggcagtg gatgccgttc cccggatcac
     5581 ccgggcccaa tctatggacg ccctgtcggc catgagcacg atcgccggat acaaggctgt
     5641 cctcctggcg gctgaccacc tgccaaagtt cttcccattg ctgatgacgg cagcgggcac
     5701 gatcgcgcca gcgaaagtgc tggtgatcgg cgcgggtgtc gcgggcttgc aggcaatcgc
     5761 aactgcgcgg aggcttggag cggtggtcga agcctacgac gcacgccccg tagtgaagga
     5821 acaggtcgag agccttggcg cacgattcgt tgagatcgat accgggtccc gggatgccga
     5881 ggctgccgga gggtatgcga gggcagcgac tgaagtagag cttcggctcc agcaagaggg
     5941 cctcaataca cacgtcgcca agtccgacgt cgtcatcacc acagcgctcg tgccgggcag
     6001 gccagcaccg cgattggtgc ctgcgtcggc agtgatatcc atgcgcacgg gcagcgtcat
     6061 cgttgacctg gccggtgagg caggcggcaa ctgtgaattg tccgtgccgg gagagacggt
     6121 ggtgcgtgaa ggagtaacga tccttgctcc actcaacatg ccgagcacca tgcccgtaca
     6181 cgcgagccag atgtactcgc gggtgatcca gaacttcctc ggactcctga tcaaggacgg
     6241 agaagtcaac ctcgatctca acgatgccat catcaagggc atgtgcatta cccatggcgg
     6301 ggatgtgatc caggagcaaa cgcggcagtt gatgaatctc ggcacgattg aagccccggt
     6361 gcccaggaac ccggcgcaac ccgagcctca cgttgagcag gacagcgccc cggccgaaga
     6421 taccgacgaa tccgagagtg aggcggaatc ggtcgtcatg ggggaggccg ccgtcgaggg
     6481 gggcgatacg agagagggcc gcccggtttg agtgcgttac cggggccagg caggggaaaa
     6541 ctgggacagg ctttgatcaa gggagacacc gatcgtggac agtgacttca tgctgggcat
     6601 ctatatattg accctcgcga tttttctggg ggccgtgctg atcatgcggg tgccatccac
     6661 cttgcacaca ccgctcatgt cggcaacgaa catgatctcc ggaattgtgt tgatcggtgc
     6721 gatgctggtc gtcggatcgg tcgatagctg gtggctgcag gttctcgggt ttctcgcgat
     6781 cgcctttagc gccgccaatg ttgttggtgg atatgcggtc acggaccgga tgttgaagat
     6841 gttcaagcag cgtgccccgg cagctggcac gagtgggaag ggggagcgat agcatgatcg
     6901 ataactggcg cgatgcgttc atcgacctcg cgtatttcat ctcggcggtt ttgctgatta
     6961 tttccctgaa ccagcttggc tctccaaaga ccgcccgtgt cggtaaccag cttgctgttg
     7021 gcggcctgct gttggccatc gtagcgacgt tcttcaaccg ggacatagcc ggcggcgact
     7081 actgggtcat cctcctcggc atcgccgtcg gtggcgctgg cggctatgtg atggcccaac
     7141 gtgtccagat gaccgcaatg ccgcagatga ttgccatctt caacggcatg ggtggggcca
     7201 cgtcggcact gatcgccgta agtgagtttc tcggcaagag cgatctcggc cgcggcaccg
     7261 ttctctcgat tgcgcttggc tgcatcatcg ggtcaatcgc cctcacggga agtctcgtgg
     7321 ccttcgcgaa acttcaggga ctggtgccga gccggacagc cctgcccgga aatcgccagc
     7381 tcatcaccgc cggtttgctc ggcggcatgg cgcttctcgg ggcaatcacc gtcatcctcc
     7441 aggatggtgc gctggcgcag acggtcctga ttctcacctt cctgcttgcc cttgcggctg
     7501 gagtcctgct cgtcatgccg atcggtgggg ccgacatgcc ggtcgtcatc gcgatactca
     7561 atgccttttc cggtgttgcg gcggtgctat ccggctttgt cgtgaataac cagctgatga
     7621 tcattggcgg aacacttgtc ggtgcgtccg gcgtgttcct gtcactcctg atggcgaagg
     7681 caatgaaccg ctcgataggc aacgtgatct ttggggcctt cggagcgaca ccctccgccg
     7741 gaggtgccgg tacgggtggc ggcgagagct tgcctgtccg cgaggttacc cccgacgatg
     7801 cggcggtctc tcttgcgtat gccgatcgtg tcatcgtcgt cccgggttac ggcctggcgg
     7861 tggcccaggc gcagcactca gtgcaggagc tcgcgtcgct gcttcaggag cgaggcgtcg
     7921 atgtcaggta tgcgatccat cctgtcgctg gccgcatgcc gggacacatg aacgtgcttc
     7981 tggcggaagc caatgttccc tacgatgact tgtatgagat ggagcggatc aacggtgact
     8041 ttgcaaaaac cgacgttgtg ctcgtgatcg gtgcaaatga cgtcaccaac ccggcggcga
     8101 gggatgatcc aaacagcccg atctacggca tgccggtcct gcgggtcgac gaggcagagc
     8161 gcgtgatcgt cctcaagcgg gggatgagca cggggttcgc cggtgtcgag aacgcgctct
     8221 tccacaaccc caagacatcc atgctcttcg gggatgccaa ggcgtccatc ggcaagctca
     8281 tagccgaggt ccgggcggcc tgacgcactc gtctgtcgtt caaagcaaag caccggtcgt
     8341 cacatatagt gacgaccggt gctttgtatt tcatggcatc ccgactgcgt tgacgagaaa
     8401 gttcgcaggg gtcattccct tgatatcagc gcagttctcc gatggaaaat gagatgaaag
     8461 cggagtatgg ttcgtgtcgg gacaggagtg ggggaaaggc ttgctgcatc aggagcgatg
     8521 cctacaccgg ctcttccggg attcccgctt tggtcgcccg ccaaggagtg gccaatagaa
     8581 tcgataaatc ctcagatacg acaagagccg cgtcagcaac cccgatgcgg cttcagtaac
     8641 ctacgagacg gagcacaatg tggtcatcaa gtcccaggag tctcgtgtcc aagatgcact
     8701 ggcacgcgac ggatccaata tcaacggtgt gatggttgag caatttgtgt cagtgggcga
     8761 cgcggtaacg ccgacgctcg atgtcaatgg gcgtccacat gtggtcgtga tcggcgcggg
     8821 gttcggtggg ctttctaccg ttcaggccct caggaacgtg gatgtggatg ttaccctcgt
     8881 cgatcgacgt aatcatcatc tttttgtgcc gttgctctat caggttgcaa ccgcccagct
     8941 ctcggcggac catatcgcac agccaatccg caggattctg aagaaacagg ataacgcgaa
     9001 ggtcgtttat gacgaggcga cagatattga tgtggacgga aagtcggtcg tcctcgccag
     9061 cggcaagacc ctgacctacg acttcctagt gattgcagtc ggtgcaaagg actcctactt
     9121 cgggcatgac gagtgggcca agcatgcccc gggcctgaag agcctggagg atgcagagcg
     9181 catccgtcag cagatcctgc ttgcattcga gcgcgcagag aaagaacggg acccggtgaa
     9241 gcgcaaggcg ctactcactt tcgtcatcgt tggtggcgga ccgaccggtg tcgagatggc
     9301 cggtgcgatc gccgagattc gaaaccatac tctccagcgg gaattcagta acttcaatcc
     9361 acgtgacgcc cgcgtggtcc tgctggaggg catgccgcgc gttttcccga tgttcaagga
     9421 gaagctgtcg gctcgggccc agaaggatct tgagaagctt ggagtcgagg tccgaaccgg
     9481 ctcgcttgtc accgacattg atgaacaggg tgtcgtgatc ggtggcgaag agcgcatcga
     9541 gaccgagacg atcatctggg ccgccggcat tcaagccgcc tcgctgacct cgtccctgaa
     9601 tgacgtgacc cagttagatc gctccaaacg tgtggcggtt acccgcgagc tgacggttcc
     9661 cggtcacccg gaaatcttcg tgatcggtga cgcagcgatc attaagtctg ctgaagacaa
     9721 gccttggggt gccgtggcgc ccgtggcaat tcagagtggc aagcatgccg cgaagaatat
     9781 tcagcgcttc atgcgtggtg aagcgttcac gccgttcgtt taccacgatc agggtaatat
     9841 ggccacaatc gggcgcaatc atggtgtagc agatatctat aatatgcaga tcaccgggtt
     9901 cgctgcatgg ctgctgtggg cgcttgtcca catcatgcag ttggtgacgt tccggaacag
     9961 ggtggcagtt gctatccaat gggcaataga ttacttcggg tacaaccgga acgcgcgcgt
    10021 tatcctcgac cactcggggg ataccaagta ggtatcgtct cagacggctc cttggtgcgc
    10081 tttggtgcgg ggtaaaggcc atgcgcgtgt ggcgggacat cgtgtgtcac tggtaacaga
    10141 atcgggttgg aggaaaagcg aggagcaaac actcctcgct tttcccggct ttgttgcctt
    10201 atcccgtcag atgcgacgct gctgatcgat cgcgaccgtc accgcatcca ggacatcatc
    10261 caaatcaaac ggcttgagga gggcggtgta gccggcatcg gcgaggtctt gggcaatgtg
    10321 cggcaggatc gtcgtgctaa tcaggggaat gcccttggta cgagtctctt ccctaagaat
    10381 atccatgaga tcggggccag tcgtcaagcg attaaacgtg gcatccagca tgatgatgtc
    10441 cggctggagg tcctggatct cctcaaggcc aagtgggctg gaggagagtg tcacatcata
    10501 cgcttcgtcg ccaagtagtt gcccaaggat gtcaaggatc tcgagattat cgtccataac
    10561 caggacgtgc gtcgtcccgg gagaggtcga acgggcctcc cagctagtga agacttttcg
    10621 ctgcgtcgct ccttcagcgt gaagcatccg cacaacgtaa cccggaagat cattccgctc
    10681 atcagatatc ccgtcaggga gtgctatatt ggacgcaacc catcgaggag gaaagggaat
    10741 aacggcgttg aactccgatt cgtctcgctt ttcattatgt cggctcatac ggaactccat
    10801 gtagtcgtcg ctgcggtgga acccgtccag gacgagagcc aacaacgcga acctaaatcc
    10861 tgaatgcagg gtcggattgc gtccatagcg cattgtgtct atgcatgaaa tgcatattgc
    10921 ctgttttgaa gcggcggtgt ggtgatggtg gagagccagg atagagaaaa ggtcatgttg
    10981 ggccagttca tccgggaacg aagggtagag ctgggcctca cgcaggaaca acttgccgaa
    11041 cgtgtgggag ataacgtccg tcaggcggaa atctcgcggc tggagcggga ccgcgtctcg
    11101 ctgccacggc gggcaaggat ggagcggctg gccaaggccc ttgaggtctc gcttgggaca
    11161 ttactgttac gaacgggctg gatggatgag gaacagagca tccgcgatgc actggtccat
    11221 atgcccgcgg ccatacgccc tcctgcccct tacctggagc cggtcatcgg cgaaacctac
    11281 gagccaaaca ggtctggtga ggaaattatc ctcctcaacg cagccctctc tcgagcccgg
    11341 gaggtggccc gccgaactga ggatgtcctc cgcgagtcca cgatgacagt ggatcatgcg
    11401 cggcgtacgc ggcgacaacc gtaatcgctg gtctgtccca tgctagggac tcctgtagtg
    11461 aatcgacgca ccggcgaggc tgcccggcaa gacccggacg agtgattcag gggcctgcgt
    11521 gctggcggag cgatctcagg agtgtggtat ggagcaaggc ggatcgccga tcgtcgttat
    11581 cggtgcctcc gcagggggaa tccaggcgct gctcgcgctc gttgcggaac ttccggcgga
    11641 ttttaatgca ccaatctttg tcgccgtaca tatcggacgc tcgcggagcg tattgccctc
    11701 cctactggag cgtgggggag tgctggccgc gtctcacgcg aaggatggtg aggtgtttcg
    11761 gcccggccac atctatgtcg gtccgccaga ttggcatctg ctcgtccgcg accactccat
    11821 acagttctcc catggcccgc gtgagaatca cagccggcct gccatcgacc caatgttccg
    11881 gtcggcggca cacgcccatg ggccgcacac gtcggtgtca ttctctcagg tgcactgagc
    11941 gacggcacgg ccggtctcat gatgatcaag gctcacggcg gcaccgcaat cgtacagcat
    12001 ccggaagagg caattatcgg cggtatgccg gaaagcgcgc tgcgagcggt gcaggcggac
    12061 cacgtcttgg ccgcgcggga aatcggccag ctgctcgcat cagagcccgc acaggtaggt
    12121 tcacgacagg agacggcaca gatggcactg gaggaggata ccgtcgagga agtgatccag
    12181 cgggatatgc gggcgcagga aaacgaccag cgagcaggcc agctcacgat gttcatctgc
    12241 cccgattgcg gtggagcact gtggcaggct gagtccactc cgtggacgag gttccggtgc
    12301 catgttggcc acgcatgggg cgtcgagtcc ctgcttggta acaagtcgga ggagctcgaa
    12361 gctgccctgt ggtccggggt gcgattgctg gaggagcgcg caacattgag ccgtcaactg
    12421 gcggctcagg tacgccagat gagccgcgat gaacatcggg cgaaggggat tgaggagcag
    12481 gccgcactgg atcaagggcg ggcgaacgct atcagggagc ttctgcatgc cccctgaatc
    12541 tcgccttgca gatggtggaa tccgcagggt cgctcggcga ggactagcgg agataccggg
    12601 ctggtggtga tgtcccggcg tggagcaggc ttcttgtcat ggacaggcag gacgagtcgg
    12661 tggaacccat tgggcattct gggccgcaag ccccaggcct cgttgtcatt ggtgcttcgg
    12721 cgggcggtgt agaaagcctc tcagcggtcg tttcgacatt acccgcggat ttcccggccc
    12781 cggtcgttgt cggcctccat ttgagcccaa gtcgggtttc ctccttgggc gatattctcg
    12841 ctcgccgggg accactgccg gtgcagacga tcagcgaccg cgtcgctctg gaaactg
//
LOCUS       DASYWM010000229         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_272422, whole genome shotgun sequence.
ACCESSION   DASYWM010000229 DASYWM010000000
VERSION     DASYWM010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272422"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VGR29_09305"
     CDS             <1..1180
                     /locus_tag="VGR29_09305"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2073823.1"
                     /translation="QDSAFAPTIVPFAQPVDAGPWRLTIVDGQLGEAAYSTIMATNDG
                     NEQAPEGLAWALAYITAENTSDQTRVINLSNFAATGTDGVLRRPPAMDCPEPALQATV
                     EPGGIAEGWIPFHVNDTGNVILWFSSPFLGGSWSQAWMALTDGAEISTFDPAPGDSGL
                     GQTPDAPAAFGETVRSGNFDVTLLEQAFGQTVYDMAEFGLRALAGSSGYDTWMAVKVR
                     ATNVSDRPAFFSYTALQLAAFNGEAWDNIMALTAPPPDAAKELMPGATREGWAAFELT
                     PWSALELVRIQPSLVADEPRFVSFGATPAAASSDTPVQEQQFSPGDQVVLTDDRINMR
                     TAASTSGDIVAELPANTALTITGEAVEADGYTWYPVEVQETGQAGYVVTDYLASAGNE
                     "
     gene            1291..1713
                     /locus_tag="VGR29_09310"
     CDS             1291..1713
                     /locus_tag="VGR29_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEV2073824.1"
                     /translation="MNFQTLALATLIVAFGVTQYTLVVQAIRDLIRRPRVRGGNKMSW
                     ALLIVCVPIVGAFLYSWMGPTSFLRHGAATATRQAAGPVLRRADELHGPRPQNVTPLR
                     PRPESSVRGRTPQQRPGLTRSKAHNTGTSVHRMRRTGS"
     gene            1725..2684
                     /locus_tag="VGR29_09315"
     CDS             1725..2684
                     /locus_tag="VGR29_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEV2073825.1"
                     /translation="MKPDSARTRVYRHRAFTGHDTTPHPENPSRVIAIDAELQRRGLL
                     HDRPAPVWEPASDEQILRVHDARLLSSLKQLVASGGGAIDPDTIVFPDSLDVARLAAG
                     AGIHAVEAIAAGEATTAAVLGRPPGHHATKVRSMGFCLLNTVAITAAHALATGFQRVA
                     VIDWDVHHGNGTQDIFYDRADVLFCSSHRYGRYFYPGTGAASERGTGAGTGFTVNIPL
                     TMGDGDAAITAALEQRILPAVRSYQPDLILISAGFDAHIEDPLGGLRVTDEGFRNLAL
                     RTRDLSRELTGGRLIAMLEGGYHPVASARCVADMIEVLDTPSP"
     gene            2858..>4147
                     /locus_tag="VGR29_09320"
     CDS             2858..>4147
                     /locus_tag="VGR29_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073826.1"
                     /translation="MVKNLFGQPRKQDSRTEQPQRQELRRHREQPQDFPSENSPSENE
                     EDRVQEVTVARPESYRRQDDRKRTEVQSQTLQGQLDDLRLVMRELSVRQQAEQETIKR
                     QAATIAQLNIALEKSRQEAQQSAQARALDENRTRQQIEDLETRLDDSTRPIRSLQAHV
                     TDLLEQSRRKVDDSTQSADRYNELMTTMEHLTAMSDRTSAIAHGLRDNIDSVRGEIDE
                     LRRDVIRSDDAVKIVDQEARRRTAAVTENIEGFTARVDELRSDLAHTYDALEDTRRGL
                     VHVDPTLDELRAGEINLRNDLSRLQSQVDERHDQLVVMQDDVRQETDARFDQLRHALE
                     ERIERLNERLEETNEIFRETTYKISEINGQIEDLRQVDASMHRDIWYLHEQRVRVRLE
                     QVQEELDVATAQRRDAEGEARVGLGNRVRRRRSSTDSS"
BASE COUNT          802 a         1337 c         1260 g          748 t
ORIGIN      
        1 gcaggattcc gcttttgctc ctaccatcgt tccctttgcg cagccggtgg atgccggtcc
       61 ctggcgattg accatcgtcg acggtcaact cggcgaggcg gcttactcca ccatcatggc
      121 gacgaatgat gggaatgagc aggcgccaga ggggctcgcc tgggccctgg cctacatcac
      181 cgccgagaac acgagcgatc agacccgcgt gatcaacctc tcgaatttcg cggcaacggg
      241 gaccgatggc gtcttgcgcc gcccgcccgc gatggactgc ccggaaccgg ccttgcaggc
      301 gacagtcgag ccgggcggca tcgccgaagg gtggatcccg tttcatgtca acgacaccgg
      361 taatgtcatc ctctggttct cgagtccctt tctgggtgga agctggagcc aggcgtggat
      421 ggcgctgacc gatggtgcgg agatctcgac ctttgacccg gctccgggag attctggtct
      481 tgggcagaca ccagatgcgc ccgccgcctt tggcgagaca gtacgctccg gcaattttga
      541 tgtcaccctg ctggagcagg cgttcgggca aacggtctac gacatggccg aattcgggct
      601 gcgagcactg gccggatcca gcggctacga cacttggatg gcggtgaagg tgcgggcgac
      661 caatgtcagt gatcgccccg ccttcttttc atatacggcc ctgcagctgg ccgcgttcaa
      721 cggggaggca tgggacaaca tcatggcact caccgcgccc ccccctgatg ccgcaaaaga
      781 gctcatgccc ggagcgacac gggagggttg ggcggccttt gagttgacgc catggtcggc
      841 gttggagctg gttcgtattc aaccatcgct ggttgccgac gagccccgtt tcgtctcctt
      901 cggcgccaca ccggctgctg catcgtcgga cacgccggta caggaacagc agttctcccc
      961 gggtgaccag gtcgtcctga cggatgaccg tatcaacatg cggactgcag catcgaccag
     1021 tggagacatt gtggcggagc ttcctgccaa tacggccctc actatcacgg gcgaggcggt
     1081 cgaggcggac ggttacacct ggtatccggt ggaggtccag gagacaggcc aggccgggta
     1141 cgttgtcacc gactatctgg cctccgccgg aaatgagtaa aaagtcccct gaacgtacta
     1201 tcgctattgc gttcagcagg ctctacactg tgagggcacg gtccgtgcaa tacgttggca
     1261 gcgaatcagg ctcatcggtc ggggaccttg atgaacttcc agaccttggc cctcgccacg
     1321 ctcatcgtgg cattcggggt cacccagtac acactcgtgg ttcaggcgat cagggatctg
     1381 atccgccgtc ctcgtgtgcg tggtgggaac aagatgtcct gggcgctcct gattgtttgc
     1441 gttccgattg ttggggcgtt cctgtatagc tggatggggc cgacaagctt tctccggcat
     1501 ggagctgcca cggcaacccg acaggctgcc ggtccggtat tgcgccgtgc cgatgagctg
     1561 catggtcccc gcccgcagaa cgtcacccct ctccgcccac gtcccgagtc ttctgtccgc
     1621 ggccgtacgc cccaacagcg ccccgggcta acgcgcagta aggcacataa cacgggaacc
     1681 tccgtccacc gcatgcgccg caccgggtcc tgaatccata tcccatgaaa cccgatagcg
     1741 ctcgaacccg cgtctatcgt catcgggcct tcaccggcca tgacaccacg ccccaccctg
     1801 aaaacccgtc tcgcgtcatc gccatcgatg ccgagttgca gcgccgggga ttgctccatg
     1861 accgtccggc gccggtatgg gaaccggcaa gcgacgagca gatcctgcgc gttcacgatg
     1921 cacggctcct ttcctcactg aagcaactgg tggcatcggg aggcggcgcg attgaccccg
     1981 acactatcgt cttcccggac tcgctcgatg ttgcccgcct tgcggcgggt gccggcattc
     2041 acgccgtcga ggccatagcc gctggggaag cgaccactgc ggccgtgctg ggacggccac
     2101 ccgggcacca cgcaacgaaa gtccggtcca tgggcttttg cctgctcaac acggtcgcca
     2161 tcacagccgc tcatgcgctt gcgacgggat tccagcgtgt cgcggtcatc gactgggatg
     2221 tccatcacgg gaatggcacg caggacatct tctacgatcg ggccgatgtc ctcttctgct
     2281 cgtcacaccg gtacggtcgc tatttctatc cgggtacagg agcagcctca gagcgcggta
     2341 ctggtgcagg taccggcttc acggtgaaca tcccgctgac aatgggtgat ggcgatgccg
     2401 ccatcacggc tgccctagag caacggatcc tgcccgctgt gcggagctat caaccggatc
     2461 tcatcctcat ctcggccgga ttcgacgccc atatcgaaga cccactgggt ggactccggg
     2521 tgaccgacga aggctttcgc aaccttgccc tgcgtacccg cgatctctcg cgggaactga
     2581 ccggcggccg gctcatcgcg atgctcgagg gcgggtatca cccggtggcc agcgcccgct
     2641 gtgtggcaga catgatcgag gttctcgata cgccttctcc gtaacccttg cggcagctca
     2701 tggacacggt acccccttcg tctcgtatcc tgtacgcgtg cacggttagg catcagtcgg
     2761 gtaccgtccc aagtgcgaca acatctccgg cacggtcggc accacttcat gcatcgcctt
     2821 gggcacgcat cccgatacga caacaaaggc agtagcaatg gtcaagaacc tcttcggtca
     2881 accccggaag caggattccc gcacggaaca gccacagcgg caggagctcc gacgtcatcg
     2941 ggaacaaccg caggatttcc cctctgagaa ctctcctagc gagaacgagg aagatcgcgt
     3001 acaggaggtc accgtcgctc gacctgagag ctatcgaagg caggacgacc gaaagcggac
     3061 ggaggtccag tcacagaccc tccagggcca gcttgacgat cttcggctgg tcatgcgtga
     3121 gctcagcgtg cgacagcaag cggagcagga gacgatcaag cggcaggcgg ctaccatagc
     3181 gcagctcaat atcgccctgg agaaatctcg tcaggaggct cagcagagcg cccaggcacg
     3241 cgcattggac gaaaaccgca cccggcagca gatcgaggat cttgagacgc gcctggacga
     3301 cagcacgcgc cccatccgct cacttcaggc ccatgttacc gatctgctgg agcagtcacg
     3361 ccgcaaggtc gatgacagca cccagagcgc ggatcgttac aatgagctga tgaccacgat
     3421 ggagcacttg accgcgatga gcgatcgtac cagtgcgatt gcccatggtc ttcgcgataa
     3481 catcgatagt gtccggggcg agatcgatga gctgcgacgt gacgtcattc gatccgatga
     3541 tgccgtcaag atcgttgatc aggaagcccg ccgtcgcacc gctgctgtca cggagaacat
     3601 cgaggggttt accgcacgcg tcgacgagct gcgatccgac ctcgcacaca cctatgacgc
     3661 tctggaggac acgcgccgcg gcctcgtgca cgttgacccg acgctggacg agctccgcgc
     3721 cggtgagatc aacctccgca acgacctctc caggcttcaa tcccaggtcg atgagcgcca
     3781 cgaccagctc gtcgtcatgc aggatgacgt gcgccaggag accgatgccc gcttcgacca
     3841 gctccgccat gcccttgagg agcgcatcga gcgcctgaac gagcgcctgg aggagaccaa
     3901 cgaaatcttc cgcgagacaa cctacaagat ctcggagatc aatggacaga tcgaggatct
     3961 tcgtcaggtc gacgcctcga tgcaccgcga catctggtac ctgcacgagc agcgtgtccg
     4021 ggtacgcctg gagcaggttc aggaagaact ggacgtggcc actgcccagc gccgggatgc
     4081 tgagggtgag gcaagggtcg gcctcgggaa tcgcgtccgg cggcgacgat cgtccaccga
     4141 ctcgtcg
//
LOCUS       DASYWM010000230         7758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_140906, whole genome shotgun sequence.
ACCESSION   DASYWM010000230 DASYWM010000000
VERSION     DASYWM010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140906"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /gene="gpmI"
                     /locus_tag="VGR29_09325"
     CDS             <1..1303
                     /gene="gpmI"
                     /locus_tag="VGR29_09325"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEV2073827.1"
                     /translation="RTFEHTPAFVSAFERVKANGGTLHMLGLIGTGGVHAHTRHLIAI
                     LDAAEVYDVRRILVHAFTDGRDAPPTSGSEFIIPIETRLATMPEARIATISGRYYAMD
                     RDHRWERTKKAFDAVVQAQGPSAGSARDAILASYAAGVTDEFILPTVIEDGHGSAHEI
                     GESDEFIFFNFRSDRGRQLTEALSQENFSGFDRGRYIPAQHHTTTMTEYDKDLPVTVA
                     FAPEDVVYPLARVISDAGRSQFHTAETEKYPHVTFFLNGGREEPFPGEVRRMVPSPKI
                     ATYDLQPEMSAPEVCKGVIEAIRSHDYDFVIVNFANGDMVGHTGVIPAVVKAIETVDR
                     CVGEIVTALLEVGGLGLITSDHGNAEQEIDPETGGPLTSHTTNPVPVILVAPDDHPLR
                     DATLRDDGVLSALAPTVLDLMGIPVPATMTQPSLIVREPGQ"
     gene            complement(1360..2643)
                     /gene="eno"
                     /locus_tag="VGR29_09330"
     CDS             complement(1360..2643)
                     /gene="eno"
                     /locus_tag="VGR29_09330"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV2073828.1"
                     /translation="MDCVIEAIYGREILDSRGNPTLEVEVFLGSGSVGRAAVPSGAST
                     GAHEAIELRDGDQERYGGKGVLQAVSNINEELAEVVVGLDAFDQVGIDNLMREVDGTD
                     NKARIGANAMLGVSLAVARAAAEEAGLPLYRYLGGPNARTLPVPMMNILNGGQHAIGS
                     SVDMQEFMVVPVGARSFTEGLRWGAEIFHTLKKILASRGLNTNVGDEGGYAPSLDSNE
                     DAVKLVTEAIEKAGYAPGEQVMIALDPASTEFYEDGAYNLKGEGRTLSSDEMVNYWAD
                     LVQRYPIVSIEDGLAEDDWGGWTSLTAMIGERTQLVGDDLYVTNTKRLRRGIDEEAGN
                     AILVKLNQIGTLTETLEAIEVAHRHGFHAIISHRSGETADTFIADLAVATNAGQIKTG
                     APSRVDRVAKYNQLLRIEDELGDISIYPGRSALRG"
     gene            3063..4124
                     /gene="pheS"
                     /locus_tag="VGR29_09335"
     CDS             3063..4124
                     /gene="pheS"
                     /locus_tag="VGR29_09335"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV2073829.1"
                     /translation="MTETQHGLAQDIENLRRRAMTEMEVLSDAAAASDWEKEYLGGKG
                     AVTALLRGLGGLPAEERPAAGRATQALRNELIESFAPIRGRLDAQALQQRLGSDAVDV
                     TLPGRVPTIGGAHPISSMIDELVEIFALLGFQAVFGPEVETGRYNFDLLNIPADHPAR
                     DVWDTIIVDSPSEMVLRTHTSPMQARTMERTEPPVRVIVPGRAFRFEAQDASHEWMFT
                     QLEGLAVDKHITLADLKGVLQEMARQLFGPERQVRFRCDFFPFVEPGVDFAVDCAICN
                     GKGCRVCKHTGWLEIGGAGMVHPKVLSGVGYDPEQYTGFAFGLGIERLIMMKYGVDDA
                     RAFAGNDLRFLRRFASTGL"
     gene            4141..6588
                     /gene="pheT"
                     /locus_tag="VGR29_09340"
     CDS             4141..6588
                     /gene="pheT"
                     /locus_tag="VGR29_09340"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV2073830.1"
                     /translation="MNVPYRWLQDLVPLDLSPEDIARRMTMAGLEAEKIQRIGAEWDK
                     VYVAEVTKVTPHPDADRLVLAEVDAVDHQLTVVTGAPNIAEGQKVALALAGARLVDAY
                     ADELKYKTLKPGTIRGVRSEGMVCSEKELGISEEHEGILVLEPEAPKGMPLQEYLGDA
                     VIEFEITPNLVHAFSILGIAREAAAIVSTRVHQPEVADISSIEPSTNLVRVDAPDLCA
                     RYSAIVMDGVNVAPSPAWMTRRLTAAGLRPVNNLVDITNYVMLELGQPLHAFDLRNVS
                     GEKIIVRRAYDSEVIETLDHVKRTLGPGDLLITDAEKAIALAGVMGGVNSEVTDDTTS
                     ILLESANFDMVSVRQTARRLKLRTDASARFERGLDPELAWQANVRAAQMVMDLCPGAT
                     VRTWDDEYPRPVEPWTVSLPLSKIALVLGMEIPKETVLDVLSRLGFAPTIDSHDTLTV
                     QVPTWRSDVTIAEDVIEEVARIVGYEALPATLITGETPAVERDPMFMLERDIRKTLIG
                     AGGYEGRTYVTVSERDIERWSLKPTGGLTYAVTGDNLLRLKNPINAEQPLLRTSLVPA
                     LISAVADNLKHQKTVRLFETGHVFIGTGADTLPSEPTLLGLAWAGFREPFDRFNAHPD
                     ANDQIDFFDVKGAIEAVLDRTGIAEAVWEAGNHSALHPGRTARVTINDETLGIVGEVR
                     PDLARGLGVSDYRLVIAELDLSTVLPLRQDASSPSIKVDRFLPVEQDFAVIVDKGTTA
                     AAVEDALRGNAGPLLTRIVLFDVFEGEQIGEGKRSLAYRLTFTAPDRAMSDAELGKVR
                     KKIERGLKSQVNGSLRT"
     gene            6599..>7758
                     /gene="hisD"
                     /locus_tag="VGR29_09345"
     CDS             6599..>7758
                     /gene="hisD"
                     /locus_tag="VGR29_09345"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEV2073831.1"
                     /translation="MAQPIKIARLSGPGTQKQLLRRGEQPEPALSRSIQERNAEIFGR
                     PITAHEVVDQIVRDVQSEGDTAVRRYTEIFDQRTLEELEVPRTERNRALAALGPELRD
                     ALKIAAGRIRSFHERQVRQSWFEAEELGVFGQIVRPLERVGIYTPGGSAALPSSLLMT
                     AIPARVAGVEEIIVAAPPRGENGISNVILAAAAIAGVDRVFAIGGAQAIAALAFGTES
                     VPQVDKIYGPGNIFVALAKQRVSGTVAIDQIAGPTETLLIADETADIEFVAADLIAQA
                     EHDPLASAILITTSPGLADSIPAEISRQAAELEREKIIGASLENNGGIVLVDSLEEAI
                     DCANGYAPEHLCLLVKDPWALVPRVRNAGGVFIGESSPEALGDYTAGPSHVMP"
BASE COUNT         1546 a         2331 c         2357 g         1524 t
ORIGIN      
        1 gagaacgttt gaacacactc ctgctttcgt cagtgcgttc gagcgggtga aggcgaatgg
       61 aggcaccctc catatgctgg ggctcatcgg cactggagga gtgcatgcac atacgcggca
      121 cctgattgcg atccttgacg cggccgaagt ttacgacgtt cgtcgcatcc tcgttcacgc
      181 atttacggac ggccgggacg caccgcccac cagtgggagt gagttcatca tccctatcga
      241 gacacggctg gcgacgatgc ccgaggcgcg cattgcaacc atttccggcc gttactatgc
      301 gatggaccgc gatcaccgct gggaacggac gaagaaagcc ttcgatgccg tcgtgcaggc
      361 gcaggggccc tccgcaggat cggccaggga cgcaatactg gcgtcttacg cagcgggtgt
      421 cacagatgaa ttcattcttc ccacagttat cgaggatggg catggatcgg cacatgaaat
      481 tggtgaaagc gatgagttta tctttttcaa tttccgctct gatcggggcc ggcaactgac
      541 ggaggccctg tcgcaggaaa acttcagtgg tttcgatcgt ggtcgatata tcccggccca
      601 acatcacacc acgacgatga ctgaatatga caaggaccta cccgtcaccg tcgcgttcgc
      661 tccggaggat gtggtctatc cgctggcacg ggttatcagc gatgccggca ggtcgcagtt
      721 ccacacggcc gagaccgaga agtacccaca cgtcacgttc ttcctcaacg gcggacggga
      781 ggagcccttt ccgggtgagg tgcgccggat ggtcccctcg ccaaagatcg ccacatatga
      841 cctccaacca gagatgagtg ccccggaggt ctgcaagggt gtcatcgaag ccattcgctc
      901 tcacgactac gacttcgtga tcgtgaattt tgccaacggc gacatggtcg gccacactgg
      961 agtgattccc gcagtggtga aggcgattga gacggtagac aggtgtgtcg gtgagatagt
     1021 gactgcactg ttagaggtcg ggggcctcgg tctcatcacg tccgatcacg gcaacgctga
     1081 gcaggagatc gacccggaga cgggcggtcc cctgacctcg cataccacca accccgtccc
     1141 cgtcattctc gtggcaccgg acgatcaccc actccgggat gcaacccttc gggatgacgg
     1201 ggtactctcg gccctcgcgc cgacggtgct cgatctcatg gggattcctg tacccgcaac
     1261 aatgacgcaa ccaagcttga tcgtgcggga gcccggtcag taagaggcgg agaaccctga
     1321 cgatctcgtc cgggctcccc aacgtgcgcc cagtcgactc tagccacgga gcgccgagcg
     1381 ccctggatag atcgaaatgt caccgagctc atcttcaatc cggaggagct ggttatactt
     1441 ggcgactcga tcgacgcggg agggtgcccc cgtcttgatt tgacccgcat tggtggcaac
     1501 ggcgagatcc gcgatgaacg tgtccgccgt ctctccggaa cggtgcgaga tgatcgcgtg
     1561 aaagccgtgg cggtgtgcga cctctatcgc ttccagtgtc tcggtcaggg tcccgatctg
     1621 attgagcttg accaggatcg cgtttcctgc ctcctcgtcg atgccccttc gaaggcgctt
     1681 ggtattcgtc acatagagat catcacccac aagctgggtg cgctccccga tcatcgcggt
     1741 taaagaagtc cagccacccc agtcgtcctc agccagtcca tcctcgatcg aaacgatggg
     1801 atatcgctgg acgagatccg cccagtaatt gaccatctcg tcggagctga gcgttcgccc
     1861 ctcccccttc aagttgtagg caccatcctc gtagaactcc gtcgaggccg gatcgagcgc
     1921 gatcatcact tgttccccgg gagcgtatcc cgccttctcg atggcttcgg tgacgagttt
     1981 tacggcgtcc tcgttcgagt cgagactggg agcatagcca ccctcatccc ctacattggt
     2041 attgaggccg cggctcgcga ggatcttttt cagcgtgtgg aagatctccg ccccccaacg
     2101 gagcccctct gtgaagcttc gtgcaccgac gggcaccacc atgaactcct gcatgtccac
     2161 gctggagcca atggcatgct gaccgccatt gaggatgttc atcatcggaa ccggaagggt
     2221 gcgtgcattc ggtccgccca aataacggta caggggaaga cccgcttcct cggcagcggc
     2281 acgcgcgaca gctagggaca caccgagcat ggcattagca ccgatccgcg ccttgttgtc
     2341 cgtgccatcg acctcacgca tcaagttgtc gatgcctacc tgatcaaatg cgtcgagccc
     2401 gacaacgact tccgccagct cctcgttgat gttggagacg gcctgaagca cgcccttgcc
     2461 gccgtacctc tcctgatcac catcccgtag ctctatagct tcgtgggctc ccgttgaggc
     2521 gccagaggga accgcggcac gcccgaccga gccgctgcca aggaagacct cgacctcaag
     2581 cgtgggattg ccacgggagt cgaggatttc tcgtccgtaa atcgcctcaa tcacacaatc
     2641 catcgttccc tccatagcca catcccgcga agaataagtc ttgcggcctc cagtataggc
     2701 cgcgttgtct cgaaggatgc ttcgctttgc tacaatcgtc agcaaatgaa gaatgtttgg
     2761 cgtcgacgga gacagcacgt accggttccc cgtccagaga ggtttggcga tcagctgaga
     2821 gccatcccgg atcccggtcg atgattccct cctgagctgt gtggtgaacg gatcttccca
     2881 ggtagtcaca cgggttgctc ccccgtagca gagccgagtc cgcgagtagc ggatcgaaca
     2941 agcgcgaccg ggccctggtc tggttgaagc agggtggtac cgcgtggatg cccctcgtcc
     3001 ctgccggggg gctttgctgt gcctctggtg aaccactatt gaaatcgagg gcaatcggac
     3061 ccatgactga aacacaacac ggattggctc aggatatcga gaacctgcgc cggcgcgcga
     3121 tgacggagat ggaggtactg tcagacgctg cagcggcctc ggattgggag aaggaatatc
     3181 tcggcggcaa gggtgccgtg acagcgcttc ttcgcggtct gggaggacta ccggcagagg
     3241 agcggccagc ggctggacgc gccacgcagg ctcttcggaa tgagcttatt gaatccttcg
     3301 ccccgatcag gggccggctg gacgcgcaag cgcttcagca acggctgggg agcgatgcgg
     3361 tagatgtgac gcttcctgga cgagtgccga cgattggtgg tgcgcatccg atctcgagca
     3421 tgatcgacga gctcgttgag atctttgcgc tcctgggatt ccaggcggtc ttcgggcctg
     3481 aagtggagac cggccggtac aacttcgacc tgctcaatat cccggctgat cacccggcgc
     3541 gcgatgtttg ggacacgatc atcgtcgact cgccatcgga gatggtgctt cgcacgcaca
     3601 cctcgccgat gcaggcgcgc acgatggaga ggaccgagcc gccggtgcgc gtgatcgttc
     3661 caggccgtgc attccgcttc gaggcgcagg acgcctccca cgagtggatg ttcacgcagc
     3721 ttgaaggtct tgctgtcgac aagcacatca cactggccga cctcaagggc gtgcttcagg
     3781 agatggcacg acagctcttc ggtccggagc ggcaggtacg ctttcgctgc gacttcttcc
     3841 ccttcgtcga gcctggggtc gacttcgcgg tcgactgcgc gatctgcaat ggcaagggct
     3901 gccgcgtttg caagcacacc ggttggctcg aaataggtgg ggccggcatg gtgcatccga
     3961 aggttctttc cggcgtgggt tacgaccctg aacagtacac agggtttgcc ttcggcctgg
     4021 ggatcgagcg gctgatcatg atgaaatacg gcgtcgacga cgcccgggcc tttgccggca
     4081 acgatcttcg attcctccga cgattcgcgt ccaccggact ctagaaggac cagacacgta
     4141 atgaacgttc cttatcgctg gcttcaggat ctggtgcccc tcgatctttc acccgaagat
     4201 atcgcgcggc gcatgacgat ggcggggctg gaagcagaga agatccagcg tatcggtgcc
     4261 gaatgggaca aggtgtatgt tgcggaggtg acaaaggtca caccccatcc cgatgctgac
     4321 cgccttgttc ttgctgaagt agatgcggtc gatcaccagc ttaccgtcgt tacgggtgcg
     4381 ccgaatatcg ccgagggcca gaaggtggcc ctcgcgctcg ccggtgcgcg gctcgtcgat
     4441 gcctacgccg acgagctcaa atacaagacg ctgaaaccag gcaccattcg gggtgtgcgg
     4501 tccgaaggaa tggtctgttc cgagaaggaa ctgggcatct cggaggagca tgaggggatt
     4561 ctggtcctgg agccggaggc gcccaagggc atgcctctcc aggaatatct gggcgatgcg
     4621 gtgattgagt tcgaaatcac gccgaacctc gtgcatgcat tttccattct tgggatcgcg
     4681 agggaggcgg cagcgatcgt cagcacgaga gtccaccagc cggaggtcgc ggatatctcc
     4741 tccatcgagc catcaacgaa tttggtccgt gtcgacgcac ccgatctctg tgctcgctac
     4801 tcggcgatcg tcatggacgg tgtgaacgtc gcgccgtcgc cggcctggat gacacggcgg
     4861 ctgacggccg cagggctacg cccggtcaac aatctcgtcg atatcacgaa ctacgtgatg
     4921 cttgagcttg gccaaccgct gcacgccttc gatctcagga acgtcagcgg cgagaagatc
     4981 attgtgcgcc gtgcctacga cagcgaggtc atcgaaacgc tcgaccacgt gaaacggacg
     5041 ctgggtccgg gtgacctgct cattactgac gcggaaaaag caattgctct cgctggggtt
     5101 atgggaggtg tcaatagcga ggttactgac gacaccacgt cgattctgct cgagtcagcg
     5161 aactttgaca tggtttccgt ccgacagacg gctcgccgtc tcaagctgcg gacagacgct
     5221 tcggcgcgct ttgagcgcgg cctcgatccg gaactcgctt ggcaagcgaa cgtccgtgcg
     5281 gctcagatgg tgatggacct ctgtccgggc gcaacagtcc gcacctggga cgatgagtat
     5341 cctcgtcccg tcgaaccctg gacagtatct ctacccttga gcaaaatcgc tcttgtactc
     5401 gggatggaga taccgaagga aacagtgctt gatgtcctgt cgcggctcgg ctttgcgccc
     5461 acgatcgact cccacgatac gctgaccgtc caggtaccga cctggcggtc ggacgtgacc
     5521 atcgcagagg acgtgatcga ggaagttgcg cggatcgtcg gttacgaggc tctcccagca
     5581 accctgatca ccggcgagac gccggccgtt gagcgcgatc cgatgttcat gcttgagcga
     5641 gatatcagaa agacgctcat aggcgccggc gggtatgagg ggcgcacata tgtcaccgtg
     5701 tcggaacgag atatcgaacg ttggtcgctg aagccaactg gcgggctgac atacgctgtg
     5761 acaggcgaca acttgttgcg cctcaagaac cctattaacg ccgagcaacc cctcctgcgc
     5821 acgagccttg ttcccgcgct catttccgcc gttgcagaca acctgaaaca tcagaagacg
     5881 gttcgtctct ttgaaacggg tcatgtcttc atcggtaccg gggcggatac gcttccaagc
     5941 gagccgacac tgctgggcct cgcatgggct ggcttccggg aaccgttcga tcgcttcaac
     6001 gcccatcccg atgccaatga ccagatcgac ttcttcgacg tgaaaggcgc tatcgaggca
     6061 gttcttgatc ggactggaat cgcggaggct gtctgggagg cgggcaatca cagcgccttg
     6121 catcccggac gaaccgcccg ggtcaccatt aacgatgaga ccttggggat cgttggagag
     6181 gtgcgaccgg acctggcacg cggtctgggc gtcagcgatt atcgtctcgt catcgccgag
     6241 cttgatcttt cgacggtgct tccactgcga caagacgcca gcagcccctc tatcaaggtg
     6301 gatcgcttcc tcccggtcga gcaggacttc gccgtcatcg ttgacaaggg cacgacggcc
     6361 gcggctgtgg aagacgccct gcgagggaac gccggcccgc ttctcacccg gatcgtgctc
     6421 ttcgatgtct tcgaaggcga gcagatcgga gagggaaaaa ggagccttgc atatcgactc
     6481 accttcactg cgcctgaccg ggcgatgagc gatgcggagt tgggcaaggt acgcaagaaa
     6541 atcgagcggg ggctcaagag tcaggtgaac ggctcgctac ggacgtaagg aggccacaat
     6601 ggcccaaccc atcaagatcg ctcggctatc gggtcccggg acacaaaaac aactcctgcg
     6661 ccggggagaa cagccggaac cggcattgtc gcggtccatc caggaacgca atgccgaaat
     6721 tttcggacgg ccgattacag ctcacgaagt cgtcgaccag atcgtgcgag atgtgcagtc
     6781 tgaaggggac actgctgtcc gacgctacac cgagatcttc gaccagcgaa cactggaaga
     6841 gctggaggtg ccccgaacgg agaggaaccg ggcactggcg gccctgggtc ctgagctccg
     6901 cgacgcgctg aagatcgcag cggggaggat acgctcgttt cacgagcgac aggtccggca
     6961 atcgtggttc gaggcagagg agctgggagt ctttggccag atcgtccgac cgctcgagcg
     7021 ggtcggcatc tatacgcccg gcgggtctgc cgccctcccc tcctcgctcc tgatgacagc
     7081 gattccggcg cgtgtagcgg gcgtggaaga gatcatcgtt gccgcccctc cccgtggaga
     7141 gaacggcatc tcaaacgtga ttctcgccgc cgctgcaatc gccggtgtcg accgtgtttt
     7201 cgccattgga ggcgcccaag ccattgccgc gcttgcattt ggcactgaat cagtgccgca
     7261 ggtggacaaa atttacggac cgggcaacat ctttgtcgcc cttgccaagc agcgggtttc
     7321 aggcaccgtc gcgatcgacc agatcgctgg tccgaccgaa acgctgctta tcgctgacga
     7381 aaccgccgac atcgagtttg ttgcggccga tctcatcgcc caggcggaac acgaccctct
     7441 cgcgtctgcg atcctcatta caacgagccc cgggctcgcg gattctatcc cggctgagat
     7501 ttcccgccag gcggctgagc tagaacgaga aaaaattatc ggtgcttccc tggaaaataa
     7561 tgggggcata gtgcttgttg attcgctcga agaagcgatc gactgtgcga atggttacgc
     7621 gccggagcat ctctgtctgc tcgtaaagga cccctgggca ttggttcccc gggtcagaaa
     7681 tgcgggaggt gtcttcattg gcgagtccag ccccgaagca cttggcgact acaccgccgg
     7741 ccccagccac gtgatgcc
//
LOCUS       DASYWM010000231         6702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_103815, whole genome shotgun sequence.
ACCESSION   DASYWM010000231 DASYWM010000000
VERSION     DASYWM010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103815"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VGR29_09350"
     CDS             <1..971
                     /locus_tag="VGR29_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073832.1"
                     /translation="AYGPGQWAYLSEGNVYRLTLGNPAAREWMVEKLVEVVDETKIDY
                     FLTDHFLWGQVNPDVQSMHATDDYLTVAEGFDIILERLHERRPQVLFEHCDNGLGLPT
                     FKMVAQHVTSIGPDAVGSQYERVGTWRLSRVLPPRYLDHYVTERLAPQIPFTGPFGDY
                     EYRSQIFGGPMILMTDIMALEQGSNDWNALVRTIDLFKRIRRRVVEGKVLHLLEPQPY
                     ERVGDGWDGWDAIGSYHPETDSAVILAFRLGGDLDTRVIPVHGLDPDSRYLITFEDRA
                     DRAERSGQEIMDRGIELSLPGPRQRRNVDGLGFVRASEVIHLEAIR"
     gene            1005..3158
                     /locus_tag="VGR29_09355"
     CDS             1005..3158
                     /locus_tag="VGR29_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HEV2073833.1"
                     /translation="MSTAGISTASLGTAYARSGTHEWHIGNTRISMTLTADPGNPLRL
                     VRITLDDHRQWHASGTESLFRLATIGDQPVASDVFRPGPSRVWTEAGAVRLAIELQGD
                     ESTVAAHFQCHEDQSVIRQWLEIVPTTPLAIGRVEPFRLAIHSPELTTVHTISGVQRQ
                     GGWRADQGAYRSFMLEHQALEAPVHRASGIRSTWDDTPWMVLTGDDAATGGILMALEY
                     GGQWEFKATRDEGSPGTIAAFAPVGTVPDVSPGERWISPSVWIGAFPGDLDSAAAVMH
                     HYLRDIVLPPTDDAFPWVQYNTWFSYYCDFDAETLLAEADVAAAMGVEVFYVDAGWWI
                     GNPRRRDRFSSGLGNWTENREKFPDGLRAFADGIRSRGMHFGLWIEPERVDLRTATTG
                     SWNPDWIARRNADQYVRADWPSDTETAWLCFGHEATQAWAEQWIGDLVQDIGIRWLKW
                     DSNYWGVCTSPGHGHGTGDGEAAQLEGVYHVMERLRRRFPDLVIENCAGGATRMDFAL
                     ARQTPVAWLNDASEPAHRSRFHNAGASYLFPPGMLNAWVTESEHENVNGQDLPDPVWR
                     AVIRSRMLGAIGFSCQLVTWSEGIRAIAREEINRYKAELRPLLRSGLMYHLLPQPEIP
                     SDRLPTPDVWEAYQFSSADDGDHVILGFRNVSPRGSMPVPVNGLQQDHWYVVTADGEA
                     PSHHQGKDLMGTGVSLTSSLLASTWVTITRDGTRA"
     gene            3155..4141
                     /locus_tag="VGR29_09360"
     CDS             3155..4141
                     /locus_tag="VGR29_09360"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HEV2073834.1"
                     /translation="MTVRFSDGWRLQGMQALVLENRQLRVTVLPELGGKIWSIVSKRH
                     DREMLWHHPRMVPRLAHYGATYDNWFSGGWDEVFPNDYPVTIDGEPYPDHGEVWSLPT
                     SWHVLEATDDSVSVALEHRGITIPTRVRKILTLEADEPHLRLRYEIANEGHQPLKAHW
                     KMHPALPITAGARLHLPVGRVVVDEDFSGPFAQASFRWPHAPMQGGGNQDMRQLPDPD
                     AGDTWFLYGLELHAGYCAVSYPGEQVGFGVSFDPKVLTSVWIFGTFGGWRNLSTIILE
                     PCTGYPARLDQAIQQGSVLTLEPEATLSTTVVASVLDGEDEMSAFEDQGGAR"
     gene            complement(4183..5367)
                     /locus_tag="VGR29_09365"
     CDS             complement(4183..5367)
                     /locus_tag="VGR29_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEV2073835.1"
                     /translation="MKIDAVDLFYLSMPEVLDIGDGSQDLLLVRIVAGEYIGWGECEA
                     SPLVSIASFVTPMSHSACKPVRDSVLGQPVDSVGDIRRIGDRVRTNSLDLLQADHTLS
                     GIDMALWDVLGKLHQVPVYTLLGSPIAHPKRPYASQLFGDTAAETFDLARQVRQDGYT
                     AAKFGWGPFGLGEVADDVEQMSAAREGLGPDAALMVDVGTVWADDVARAAERLDVLKR
                     CDVTWLEEPFVTGAFDAYRTLAVQARPVALAAGEGCHNTWQARHLIDYASIGFVQIDA
                     GRIGGITPAADVARYAEARGVAYVNHTFTSHLALSASLQPFAGMANDEICEYPVTPKS
                     LALDLTREHLDRDRNGMIHVPDRPGLGMSVDIDAIRPYLVDVDIRVNGQSLYRTPDLV
                     DS"
BASE COUNT         1320 a         2013 c         2072 g         1297 t
ORIGIN      
        1 gggcctatgg tccgggtcag tgggcatacc tgagtgaggg caacgtctac cggctcacct
       61 tgggtaaccc tgccgcgcgc gagtggatgg tcgaaaaact ggtcgaggtc gttgacgaga
      121 cgaagatcga ctatttcctc accgatcatt tcctttgggg tcaggtaaac ccggacgtcc
      181 agtccatgca tgccactgac gactatctga ccgtcgccga agggttcgac atcatcctgg
      241 agcgtttgca tgagcgacga ccccaggtgc tgttcgagca ctgcgacaat ggtcttggcc
      301 tgccgacatt caagatggtt gcccagcacg tcacgtcgat cggaccagat gcggtcggat
      361 cacagtacga gcgagtcggg acctggcgcc tttcccgggt cttgccacct cgctatctcg
      421 atcattacgt taccgagcga ctggcaccgc agattccgtt cacgggaccc tttggtgact
      481 acgaatatcg gagccagatc ttcggcggac cgatgatcct gatgaccgac atcatggcgc
      541 tcgagcaggg ttcgaacgat tggaacgcgt tggtcaggac aatcgacttg ttcaaacgga
      601 tccgccgtcg ggtcgtcgaa gggaaggtgc tgcacctgct tgagccacag ccatatgagc
      661 gggtaggcga cggctgggat ggttgggatg cgatcggctc ctatcatccc gagaccgatt
      721 cggccgtgat cctcgccttt cgcctgggag gcgacctcga tacgcgagtg atcccggtgc
      781 atggacttga tccggactcc cgatacttga tcacctttga ggaccgtgcc gaccgggccg
      841 aacgatccgg gcaagagatc atggaccgtg gtatcgagct ttcattgcca ggcccaaggc
      901 agcgccggaa cgtggatgga ctcggctttg tccgggcctc ggaggtgatc catctggaag
      961 cgatccgttg acggcacagt gcgagcgaaa tgagggcaat atcagtgtcg acagcaggaa
     1021 tctccaccgc aagcctgggc actgcctatg cccgaagcgg cacccacgag tggcatatcg
     1081 ggaacacccg gatttccatg acattgacgg cggatccggg caatccgttg cggctcgttc
     1141 gcatcaccct cgacgatcac cgccaatggc acgcgtcggg cacggaatcg ctgttccgcc
     1201 tggcaacaat cggcgatcaa ccggtcgctt cggacgtctt tcgccctggt cccagccgcg
     1261 tctggacgga ggccggtgcg gtgcgcctgg cgattgaact gcagggagat gagagcaccg
     1321 ttgccgccca cttccagtgt cacgaggatc agtcggtaat ccggcagtgg ctcgagatcg
     1381 tgccgacgac cccgctcgcg attgggcgtg tcgagccctt ccgtctagcc atccactctc
     1441 ctgagctaac gaccgtgcat acgatttccg gagtgcagcg acagggtgga tggcgcgccg
     1501 atcagggagc gtatcggtcg ttcatgctcg agcaccaggc gctggaggct ccggtccacc
     1561 gagcgtcggg aattcgatcc acgtgggacg acacaccgtg gatggtcctc accggagacg
     1621 acgcggcgac cggcggcatc ctgatggcgc tggagtatgg cggacaatgg gagttcaaag
     1681 ccacacgaga cgagggctca cccggaacga tagctgcgtt tgctccggtc ggcacggtgc
     1741 cggacgtgtc accgggagaa cgttggatct cgccctcggt ctggatcggc gcctttccgg
     1801 gcgatctcga ttccgctgcc gcggtcatgc atcattatct gcgggacatt gtccttcccc
     1861 ccaccgacga tgcctttccg tgggtgcagt acaacacgtg gttttcctac tattgcgatt
     1921 tcgacgccga gaccctgctc gccgaggcgg atgtcgccgc agcgatgggg gtggaggtct
     1981 tctacgtcga tgcgggatgg tggatcggga acccgcggcg gcgcgaccgg ttctcgtcgg
     2041 ggctcgggaa ctggaccgag aaccgggaga agtttccgga tggactgcgc gccttcgccg
     2101 atgggatccg atcccgggga atgcatttcg gcttgtggat cgaacctgag cgtgtcgatc
     2161 tccgcacggc gacaaccggg tcctggaatc ccgactggat cgcccgtcgg aacgccgacc
     2221 agtatgtccg ggccgattgg ccatccgata ccgagacggc ctggctctgc ttcgggcatg
     2281 aggccacgca ggcatgggcc gagcagtgga tcggagacct ggtacaggac atcgggatcc
     2341 gctggctgaa gtgggacagt aattactggg gagtgtgcac cagtcccggt cacggacacg
     2401 gcacgggtga cggcgaggcg gcccagctcg agggcgtcta ccacgtgatg gagcgattgc
     2461 ggcgtcggtt ccctgacctg gtcatcgaga actgcgcagg cggcgccacc cggatggact
     2521 tcgcgctcgc tcgacagacc cccgtagcgt ggctcaacga cgccagtgag ccggcccacc
     2581 ggtctcgctt tcacaacgcg ggcgccagtt atctcttccc gcccgggatg ttgaacgcct
     2641 gggtgacaga atccgaacac gagaacgtga acggccagga cctgcccgat cccgtatggc
     2701 gggcggtcat tcgcagtcgc atgctgggag cgatcggctt ctcgtgtcag ctcgtgacat
     2761 ggagcgaagg catcagggcg atcgctcgcg aggagatcaa ccggtacaag gcagaattgc
     2821 gaccgctgct gcggtccggc cttatgtatc acctgttgcc acagccggag atcccctccg
     2881 atcgcttgcc aacaccggac gtgtgggagg cataccagtt ctcgtcagcc gacgatggcg
     2941 atcacgtaat cctcggtttc cggaatgtct ctccccgggg ttcgatgccg gtgccggtca
     3001 acggtctcca gcaggatcac tggtatgtgg ttaccgctga tggtgaagca ccgtcacatc
     3061 atcagggcaa ggacctgatg gggacgggcg tgtcactcac ctcttcattg cttgcctcga
     3121 cctgggtgac gatcacgcgc gatgggacgc gggcatgact gtccgtttca gcgatggctg
     3181 gcgcctgcag gggatgcagg cgctggtcct ggagaaccgt cagctcaggg tcaccgtcct
     3241 gccggaactg ggcgggaaaa tttggtcgat cgtctccaag cggcatgacc gggagatgct
     3301 gtggcatcac cctcggatgg ttccgcgact cgcgcactac ggtgccacct acgacaactg
     3361 gttcagcggg gggtgggacg aggtgttccc gaacgattac cccgtgacga tcgacggcga
     3421 accctacccc gaccacgggg aagtgtggtc gctgccaacg agctggcacg tgctcgaagc
     3481 gaccgacgat agcgtgtccg ttgcgctcga gcatcgaggc atcaccatcc cgacccgcgt
     3541 tcgcaagatc ctcacgctag aagccgatga gccgcacctg cggctaaggt acgagatcgc
     3601 gaacgagggt caccaaccgc tgaaggcgca ctggaagatg cacccggcgt tgccgatcac
     3661 cgcaggtgcg cggttgcatc tccctgttgg gcgggtcgtg gtcgacgagg acttcagtgg
     3721 ccccttcgcc caagcctcct ttcgctggcc acacgcgccg atgcagggtg gcgggaacca
     3781 ggacatgcgg cagctgcccg atcccgatgc gggcgacacc tggttcctct acgggcttga
     3841 gctccacgcc ggatactgtg cggtcagcta tcccggggag caggtcggat ttggcgtctc
     3901 gttcgatccg aaggtgctga cgtcagtctg gatctttgga acgttcggtg gctggcgcaa
     3961 tctctcgacg attattctcg aaccctgcac ggggtacccg gcccggctcg atcaggctat
     4021 ccagcaagga tcggtgctaa cgcttgagcc tgaggcgacc ctctccacca ccgtggtagc
     4081 atcggtcctc gatggggagg atgagatgtc cgcattcgag gaccagggag gagcccggtg
     4141 aatcacgagg acaccctccc gtctatgtga attgcctcgg gatcaggaat cgaccaggtc
     4201 cggcgtccgg tacagcgact gtccattgac ccggatatcc acatcgacca aatacggtct
     4261 gatcgcgtcg atatcgacgg acatgcctag ccccggtcga tccggtacgt ggatcatccc
     4321 gttgcgatcc cgatccagat gttcccgggt gaggtcgagc gccagcgatt tcggcgttac
     4381 gggatattca cagatctcgt cattcgccat ccccgcgaat ggttgtagcg aggccgacag
     4441 cgccaggtga gatgtgaacg tgtgattcac gtaggccacg ccacgcgcct cggcgtaacg
     4501 ggcgacatca gccgccgggg tgatgccacc aatcctgccg gcgtcaatct ggacgaaacc
     4561 gatgctggcg taatcgatca ggtggcgggc ctgccaagta ttatggcagc cctcgccggc
     4621 ggccagggcg actggtcgcg cctgcaccgc cagcgtccgg taggcatcga acgcgccggt
     4681 cacaaagggt tcctcgagcc aggtgacgtc gcatcgcttg agcacatcca gccgctccgc
     4741 ggcacgggcg acatcgtctg cccaaaccgt ccccacgtcg accatcaggg cggcatccgg
     4801 ccccagtcct tcacgggcgg ctgacatctg ctctacgtcg tcagccacct cacccaatcc
     4861 gaacggaccc cacccgaact tggcggctgt gtacccgtcc tggcgaacct gccgcgccaa
     4921 atcaaaggtc tcggcggccg tatcgccaaa cagttgggat gcatatggac gtttcggatg
     4981 cgcgatcggt gaaccgagca atgtgtagac tggcacctgg tggagcttgc ccagcacatc
     5041 ccaaagcgcc atatcgatgc ccgacaaggt gtgatccgcc tgcagaagat cgaggctgtt
     5101 ggtcctcacg cggtcgccga ttcggcggat atccccgacc gaatcgacag gctgtcccag
     5161 cacagaatcg cggacgggct tgcacgcact atgcgacatg ggggtgacaa agctggcgat
     5221 cgataccagg ggagatgcct cgcactcacc ccagccgatg tattcgcctg ccacgattcg
     5281 caccagcagg agatcctggc tgccatcgcc gatatccaat acttccggca tcgagaggta
     5341 gaagagatca acagcatcga tcttcacagt cagtcccccg atcaacattt caactgcaac
     5401 gcggcgccag tatatggaga ccatgcgaca tggaacagat gctggagagc acagatctcg
     5461 aggccgtggg tcacctgaac atggcaccaa attagaaaca cttgccatcg ccacgatggt
     5521 agctaatcgt agatagccgt gtgcgcacgt ttcttttgtc ccggcaagct gatgggccca
     5581 catcatgctc tctagagtgg cgcggtggaa gcttgcaaca gctcacgtga tggcttcaag
     5641 cccgcatgct ttatttagcg agaatcgctc catctcggcc ttcccccctg gcctggaaca
     5701 agcgtaggag gttctcctcg gcatacgggt acttgttggc ttctttatga tagagcgtca
     5761 cgccaagccc cgggccgtta ggtacctcga tgtagccctc aacaatctcg acgtgtccgg
     5821 acatcacttc gcggatccat ggaaccagaa aatcgtcaaa gtcgcagcga tcagcgtgga
     5881 aaagcggata aattgccgca tgattccttc gcagaggtgg ctcccaagct ctgcgaggcc
     5941 gcaaaccgga tatccttgcg gcttgggtgt catcagagct cggcatacat gccgttcaaa
     6001 tcggtctgta cccgtaaccg ccttttgtca caatgttgtc acggatgcaa gcgaatagat
     6061 ccgcacagca ctacatgtgc cgggctcatg ccgcgcccac tgctgcatac gtactcgtga
     6121 tattgcagag cgggacactg aatcgcatgg tgttcccggg atcccgcatt ccgcttcatc
     6181 tgaaccgcag aacgacgctc aaagccgcgc tcgggcttct caactgagca cagcaaacgc
     6241 tcggctatta ctgccaaatg gctcaacaga cacgcaattg agccgtgagg accgttaggc
     6301 tgcacagcgg gccagttacg gtaacgaccg ttttgctctg actcatctcg atcggtggcc
     6361 gagcatcacg agaggggtgc gaggctggat tggtgaaggc tgggaaggct tgatctattg
     6421 gcggtcactg caaggcagcg tcaccactcc atctgccgag ttggaggagg cactgcagag
     6481 ggccagtcgg aaaggatgcg gtcttgatgc tgcccgcgat tcaggccgca aggatattga
     6541 cacggagcgg gaagagaata gaataaaacc gaagcatgga tcaggttcac tgccaccgtt
     6601 gtttagttcg gacgtgttcg gattgaataa tggaggtgat ggtttatgat gcgtaacccc
     6661 ctcttcaatg acctcttgag tctgcgcaac actgtcgatc ag
//
LOCUS       DASYWM010000232         4800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_122489, whole genome shotgun sequence.
ACCESSION   DASYWM010000232 DASYWM010000000
VERSION     DASYWM010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122489"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VGR29_09370"
     CDS             <1..988
                     /locus_tag="VGR29_09370"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEV2073836.1"
                     /translation="GDREIFVVAEDGRHEITRARAIDEGGEGLDAIWADDFHHDVRVH
                     LTNAHENYYQYYKGSTTDIATAINKGFGPLTISKKDVAPVSHEDPASAFVFCIQNHDQ
                     VGNRPFGDRLHHDINADRYAVASTLMLFAPETPMLFMGQEFSVSTPFYYFTDHPEELG
                     KLVTEGRRQEFSGFSIFDDPALTQSIPDPQAESTFYGSKLKLEERQRNAGIYALYRTL
                     LALRHDDDVLKHNDRQKSHASAISAEMVAVHRWWGTEQRLLLANFGPEVRLTVKDVEA
                     FSPMFSRPWNLMLTTTDAQFGGSGEVPAITGLLGERCVTVPARSALIFAVGD"
     gene            996..1295
                     /locus_tag="VGR29_09375"
     CDS             996..1295
                     /locus_tag="VGR29_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073837.1"
                     /translation="MHRENIGRCGGSELTKAFEHRTVDDLGRLDAVLLTGDDQEFLRI
                     RFGHLNTPGTSRNRLDGYKECAGRNPPGALPFVEENCKRDTVITFPPHGKSYGND"
     gene            complement(1355..1564)
                     /locus_tag="VGR29_09380"
     CDS             complement(1355..1564)
                     /locus_tag="VGR29_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073838.1"
                     /translation="MPKQWGDLSKEEQEKFQNMDISGEEGGDTGPSGYIDDGEQAEKG
                     DTRRENPKHSGPTRNGPAGNEKQNS"
     gene            complement(1923..2774)
                     /locus_tag="VGR29_09385"
     CDS             complement(1923..2774)
                     /locus_tag="VGR29_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV2073839.1"
                     /translation="MWSTGFIGARYGVPHAEPATFLALRFAGVLVVMVPVVLCLRLPF
                     PRQVKAGHLVIAGLLIQGGYLLGVFEAVHRGMPAGVAALIVGLQPILTAVLGGLVGER
                     VTRLQWLGMALGLSGVVLVVAERLTASGLTMASVAADLGALIAITLGTLYQKRFVPAF
                     DLRTGSVIQFAAGLLLVVPVAFLAESRKIEWAPELIGLLAWSIVALSIGATSLLFLLI
                     RRGAATRVASLMYLTPAVTAVMARILFGEQLSAIMVLGIGLTALGVAIMVRAPGRHSV
                     RARAHED"
     gene            3325..4056
                     /locus_tag="VGR29_09390"
     CDS             3325..4056
                     /locus_tag="VGR29_09390"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2073840.1"
                     /translation="MASKRRLPFIALVEKSGPQPEERYVPPGKMGRGEEALMDPRQVQ
                     SVRERVFALDIEEGTIKVGVDEESSYKEPVAHIVVGFIGSRKTTFARKLEKETGAVRF
                     TKDEWMVRLFGNTPPKDKFEEYDSRMASLATDMALRCLKAGIDVVIDEGFWVKEQRDE
                     IRQRVKSVGATPKLYYLRVPLEVMKARALRRTEQPPIDSFTIDEESFNHYWRFFQPPD
                     KDEECTLIGEVIDHSGRGRSADKSE"
     gene            complement(4549..>4800)
                     /locus_tag="VGR29_09395"
     CDS             complement(4549..>4800)
                     /locus_tag="VGR29_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073841.1"
                     /translation="VPRAHHFPEFESHHTETPTYLNPMGAKGIGEAATIGATPATANA
                     VIDALEPWGITHLDIPCTPQRVWQAIRDAEKEPGASAAD"
BASE COUNT         1032 a         1354 c         1482 g          932 t
ORIGIN      
        1 cggtgaccgg gagatcttcg ttgtcgccga ggacggcagg catgagatca cccgagcccg
       61 tgcaatcgat gagggcggtg agggactgga cgcgatctgg gccgacgact tccatcatga
      121 cgttcgtgtt cacctgacga acgcgcacga gaactactac cagtactaca agggctcgac
      181 cacggacatt gcgacagcga tcaacaaggg gttcgggcca ctgacgatca gcaagaagga
      241 cgtggcgccg gtcagccatg aagatccggc atcggccttc gtcttctgca tccagaatca
      301 cgatcaggtg ggcaaccgtc cgtttggcga ccgcctgcac cacgacatca atgctgaccg
      361 gtacgccgtg gcatccacgc tgatgctctt tgcaccggag acgccgatgc tcttcatggg
      421 gcaggaattc tcggtctcga cgccgttcta ttatttcacg gaccacccgg aagagcttgg
      481 aaaactggtg acggaggggc gccgccagga gttctccggg ttcagcatct tcgatgatcc
      541 ggcgctgacg cagtcgatcc ccgacccgca ggccgagtcc acgttttatg gctcaaaact
      601 caagctggaa gagcgtcagc ggaacgcggg catctacgcg ctgtacagga cgctgctggc
      661 gctccgccac gacgatgacg tcctgaagca caacgaccgc cagaagtccc atgcaagcgc
      721 gatctcggct gagatggtgg ccgtccatcg gtggtggggg accgagcagc ggttgctact
      781 ggccaacttt ggtccggagg tccgcctcac agtcaaggat gttgaggcgt tttcgccgat
      841 gttctcacga ccgtggaacc tgatgctcac gacgacggac gcacagttcg gggggagcgg
      901 cgaggttcct gccatcactg gtttgctcgg tgagcgttgc gtcaccgttc cggcgcggtc
      961 ggccctgatc ttcgcggtag gggactagcg cgatattgca ccgggagaac atcggtcggt
     1021 gtggcgggag cgaactgaca aaagcctttg agcaccgcac tgtcgatgat ctgggcaggc
     1081 ttgatgctgt tctgctcacc ggtgatgatc aggagtttct gcgaatccgc tttgggcacc
     1141 tcaacacgcc gggaacgagc cggaaccggc tggatggata caaggaatgc gccgggagga
     1201 accctcccgg cgcgttgccc tttgttgagg agaattgcaa gcgcgataca gtgatcacgt
     1261 ttccgccgca cgggaagtcg tacggtaacg attgatcaga atcgtcatgc tgcgcgtgcc
     1321 ctcaggagtt tggacacctt cgacacatca ggggtcagga attctgcttc tcgttcccgg
     1381 cgggcccgtt gcgcgtgggt ccgctgtgtt tggggttctc gcgacgggta tcgcctttct
     1441 cggcttgctc gccgtcgtca atgtatcccg aggggccggt atctccaccc tcttcgccgc
     1501 tgatgtccat attctggaac ttctcctgct cttccttgct caggtcgccc cattgttttg
     1561 gcatggttgt cctccttgta gtcagcgcag gacgcgctcg cgattgccat gcaggttccg
     1621 ttccggggtt caccaaccga tgcgagagct agaagaagga tctcccggcg cgccggtaaa
     1681 aaccatggcg catccgactg cggcgagagc atcgttccga atgttgggga tttggtccct
     1741 gctggagacg acggcataga cagtgagcaa ttcggaggtc ccaaaacctg tattgggtca
     1801 caggcaatgc cccccttgat gatgagatgc gttgtgatgc gtacataggg gccaaagagg
     1861 gctgcctatg gcagaggagg ggatatcaga aaccaccgtt tgtgagacaa gcaacggctg
     1921 gttcaatctt catgtgcacg ggctcggacg gaatgccttc cgggcgcccg taccatgatc
     1981 gccaccccga gcgcggtgag cccgatgccc agtaccatga tggcgctcag ctgctccccg
     2041 aacaggatgc gcgccatgac ggcggtcacg gccggggtga ggtacatcag gcttgccacg
     2101 cgggtggcgg caccccgccg gatcagcagg aacaggagcg acgtcgcccc aatcgagagg
     2161 gcaacgatcg accaggccag cagcccgatc agctccggcg cccattcgat cttgcgtgac
     2221 tcagcgagga acgcgacagg caccaccagc agcaggccgg ctgcaaactg gatcacgctc
     2281 ccagtgcgga ggtcgaaggc cggcacgaac cgtttctggt agagggtgcc gagtgtaatt
     2341 gcgattagcg ccccgagatc ggcagccacg ctggccatcg tgagaccgga ggccgtgagg
     2401 cgctcggcga ccaccagcac gacgccggac aatcccagag ccatccccag ccattgcagc
     2461 cgcgtgactc gctcccccac gaggccgccg aggacggctg taaggatcgg ctggaggccg
     2521 acgatcagag cggctacccc ggccggcatc ccccggtgga cggcctcgaa cactcccagc
     2581 aggtagccac cttggatcag caggccggcg atgacaagat gcccggcctt cacttgcctc
     2641 gggaatggca ggcgcaggca gaggaccacc ggaaccatta cgaccaggac gccagcgaac
     2701 cggagcgcaa ggaatgttgc gggttcggcg tgtggcaccc cgtagcgggc gccgatgaag
     2761 ccagtgctcc acatcagcac gaacaccggg gcgatcgacc tgcccctctc cccgcagtga
     2821 agacctcagc aaggctcaac ccgagagaac gtcgcctgcc ccacctccac gcctatgagc
     2881 aagcgacacg cgactacgtg gactccttct acgaagaggt cgtcgcgcgc atgacgtccg
     2941 aacttgatac caaggatgac cacatcggct cggcgctgcc gtcacccagg ggcgcctata
     3001 tgtgtcgcca atggggcgtt acaccggagc agtttgacga gccaagcagc cctcgcccaa
     3061 caagggggtc accacctgac gcgtcggtga tagccttggc gcatccgacc gggcgagggc
     3121 gtccctctga aaagtgagga tggggtctcc gttggagacg cgagcatgac cgatacgcac
     3181 gatccaaatg tctcagaacc tgcttgggga aacaagaaac cggacgtaga ttgtgggaca
     3241 agtttcgata cgggattatg gtggttattg gacagtttca cggagccacg aacgagttcc
     3301 agaaacggat gtttaggagg tgacatggca tcaaaaagga gactgccctt catagctttg
     3361 gtcgagaaat caggaccaca acctgaagag cgatatgttc cccctggcaa aatgggtcgg
     3421 ggagaggagg cactgatgga tcctcggcaa gtacagagtg tgcgggagcg agtgttcgcg
     3481 ctagacatcg aggaggggac gatcaaggtc ggagtcgacg aggaatcctc gtataaagag
     3541 ccggtggcac acattgttgt tggattcatc ggttctcgaa aaacaacatt tgcgagaaaa
     3601 ctcgaaaaag aaacgggagc tgtcaggttc accaaagacg aatggatggt tcgactcttt
     3661 ggcaacactc ctcccaaaga taagttcgaa gaatatgaca gtagaatggc ttcccttgca
     3721 acagatatgg cactcagatg cttgaaggca gggatagatg tcgttattga tgaaggcttt
     3781 tgggtgaagg agcaacgaga tgaaatcagg caacgggtaa aaagcgttgg tgcgactcca
     3841 aaactatact atctaagagt gccccttgag gtaatgaagg caagagcact aagaagaacc
     3901 gagcaaccgc ctatcgattc ttttactatc gatgaggagt cgttcaatca ttattggcga
     3961 ttttttcaac cccctgacaa ggatgaagag tgtacgttga taggggaggt gatcgaccac
     4021 tctggccggg gccgctcggc tgacaaatca gaatgatgag cgggttgtgc cagatctacc
     4081 cattgtttgc ggacaatctt tcgtgcgcaa aacgtgtggg acgtttattg atggatacga
     4141 ctggtagaac gctgagtcag ccgactcaag ctgtatgttg aactttcatc gagcatcgag
     4201 gctcactgcg tcggcatacc tgcgtttcga ttgcctggtc atcgtcgaca accagccagg
     4261 cgtcctgttg taagggcagg gaggacaagt ccgctcggtc tcgccctcct tcgctatctc
     4321 cagggtgatc catggtaggt atgccccgaa agaccttgct gtcgaaccag gtccctcttc
     4381 gggccgatgt ccgattggtc aacccgttag gtggccacct cccctatagt gcgaaatatg
     4441 gccagcggcc aaattgtttt atgtggccaa gtgagccgct ccatgtcaac ctgcaggaga
     4501 cagcggtgtt gaggttggct caccgttggt acaatgatcg gataccgctt agtccgcggc
     4561 tgatgcacca ggttccttct ctgcatcccg gattgcctgc cagacgcgct ggggcgtgca
     4621 ggggatgtcc agatgggtga tgccccacgg ctcaagtgca tcgatcacgg cgttagccgt
     4681 cgcgggggtg gcgccgatcg tcgccgcctc accgataccc ttggccccca ttgggttgag
     4741 gtaggtcggg gtctcggtgt gatgcgactc aaattccgga aagtgatgcg cacgcggaac
//
LOCUS       DASYWM010000233         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_366333, whole genome shotgun sequence.
ACCESSION   DASYWM010000233 DASYWM010000000
VERSION     DASYWM010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366333"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            790..1143
                     /locus_tag="VGR29_09400"
     CDS             790..1143
                     /locus_tag="VGR29_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2073842.1"
                     /translation="MQTKTPPVNISICPKFHKAVELIGRRWTGAILSALLKGATRFTD
                     IIHAVPGLSDRLLSERLKELEAEGIVQRIVHPETPVRIEYQLTEKGHELNAVIATLTD
                     WAAEWVGDGDVCVTS"
     gene            complement(1187..2623)
                     /locus_tag="VGR29_09405"
     CDS             complement(1187..2623)
                     /locus_tag="VGR29_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV2073843.1"
                     /translation="MDFPDEAQGRVEQDRHRPRYHFLPPANWMNDPNGLIHYQGQYHL
                     FYQYNPKGAFWGTMHWGHALSEDLVHWRHMPVALTPAIGGPDEDGCYSGAAVVHDGVP
                     TLVYTGVRGTDQLPCLAMSEDQDLGKWRTFPGNPVIDETPSDVQTTIFRDHTLWREDG
                     AWMMGIGAGIEGSGGAVLLYCSHDLIQWEYLHPLAIEDPTLNPGGRVLSTGWECPDFF
                     FFGDRPALIACEWDGEPIAVSYWTGQYLRRRFTAHYKGAVDAGSSFYGPQSFMDESGR
                     RIMFGWLREVRSESAQRDAGWSGAMTLPRIITMGEDGALAFAVVPEVEALRGRHYRLD
                     VLNSDVDLRGVPGNSLEIQARFGAHGGGPVGLQVCCSPDGGEATTVLWDIETGILSLD
                     TRQSSRDQEVTGELASAFLGLTEGEPLSLRVFVDRSVIEVYANQHIALSGRIYPSRQD
                     SLGVRVVGPDALSGDIDLYEMKPMTTGL"
BASE COUNT          662 a          890 c          821 g          625 t
ORIGIN      
        1 gttgcagctc gtgcagcatc ttgaccgtct tcaggaaacg cgtgtgcgcc cgttccgcca
       61 accttgaact tctctcaatc gattcggcta tcacactgtg gggcgggtgc cagcgcccct
      121 cccccaccac tcccatgaca atggtattcc gagtacgtcg agtgtccgct gctcagcgaa
      181 ctcattgcag ctggttttca tcagtcgact acaaaggtga ttgggccaaa gcgattgatt
      241 tgcaaggact tcattgagac gagtaagaag gcggtagcac ggcgctcctt ctggacattc
      301 gtactgatct ggagtgagtc cggtgcgtac tgcaatctcg agttcctcgt ccgaatcatg
      361 ggataaactg tccatgtgac cgctcctggt cagtgggtgc cacggcctcg acccggatgc
      421 gaaccggtcg aggctttttt gttcaaatcg ttaccttcga ccatatcagc acgcgcgaca
      481 gggccgaatg acgctacggc ggttgtcttg ggccacgcag ggaatgatca gcgcctaggt
      541 gcaaattgag cgcaacagat ccacgctgtc gcggtgtact taatccctgg aaattgagga
      601 ggataagaga aacagcggtg tagccatttt gtgtagcaaa ctgatgtacc aagagtggcg
      661 catgtggtaa gtgttgtggg gaatattggt gcgtgaagct gctgacaagg ggttgcctgc
      721 caacctataa tgagcagtac tgggaccagc atgtacgtca ccatagaatt cagaggcaag
      781 aattcagcaa tgcaaaccaa gacgccaccc gtcaacatct ccatctgccc caaattccac
      841 aaagccgtgg agcttatcgg taggcggtgg accggtgcaa tcctgagtgc tctcctcaag
      901 ggcgctaccc gctttaccga tatcatccac gcggttccgg ggttgagtga tcgccttctt
      961 tccgagcggc tgaaggaact tgaggccgag ggtatcgtgc aacggatcgt gcatcctgaa
     1021 acaccggtgc gcatcgagta ccagctgacc gaaaagggac acgagctcaa tgcagttatt
     1081 gctaccttga ccgactgggc tgcggaatgg gtaggcgacg gtgatgtttg tgtaacctcg
     1141 tgaagcccat tttccggttc agcccctcag gtgccttgtc tcctctctac aagcctgtag
     1201 tcatcggttt catttcgtaa aggtcaatgt ctccgctgag ggcatcgggg ccaacgacgc
     1261 gtacaccgag ggaatcctgt cgcgaggggt aaatgcgccc cgagagagcg atgtgctggt
     1321 tggcataaac ctcgatcacc gaacgatcca cgaaaactcg cagtgagaga ggttcgcctt
     1381 cggtgaggcc cagaaacgcc gacgccaact cccctgtgac ctcctgatcc ctgctggact
     1441 ggcgggtatc gagcgagagg ataccggtct cgatatccca aagtacggtt gtcgcttcac
     1501 caccatcggg cgagcaacac acctgcaggc ccactggccc accgccgtga gcaccgaacc
     1561 ttgcctggat ttccagggaa ttgcccggca ctccacgaag atcaacatca ctgttcaaga
     1621 catcgagcct gtagtggcgt cctcgaagcg cctccacctc cgggacaacg gcaaacgcga
     1681 gtgcgccatc ctcgcccatg gtaataatcc gcggcagggt catcgcacca gaccaaccag
     1741 catcgcgctg ggcggactcg cttcgaactt cccggagcca tccaaacatg atccgccttc
     1801 cagactcgtc catgaaactt tgcggcccat agaaggacga gccggcatcg accgcgcctt
     1861 tgtaatgtgc tgtgaaccgc cttctgagat actgccccgt ccagtaactg accgcgatcg
     1921 gctcaccgtc ccattcgcag gcgatcaatg ccggacgatc gccgaaaaag aagaaatcgg
     1981 ggcattccca tcccgtggag aggacacgac ccccgggatt tagcgtcgga tcctctatgg
     2041 ccagcggatg tagatactcc cactggatga gatcgtgaga gcaatagagc aacacagctc
     2101 ccccgcttcc ctcgatccct gccccaatgc ccatcatcca ggcgccgtct tcacgccaca
     2161 gggtgtgatc tcgaaaaatg gtcgtctgca catcggatgg cgtctcgtcg atcaccgggt
     2221 tgccggggaa cgtccgccat ttgccgaggt cctgatcctc gctcatcgcc aggcacggga
     2281 gttggtccgt accgcgaacg ccggtataca caagcgtcgg gactccatcg tggacgactg
     2341 ccgcacctga atagcaaccg tcctcatccg ggccaccaat tgcgggagtc aacgcgacgg
     2401 gcatgtgcct ccaatgcaca aggtcctcgc tcaacgcgtg tccccaatgc atcgttcccc
     2461 agaacgcccc cttcggattg tattggtaaa agagatggta ctggccctga tagtggatca
     2521 agccgttggg atcgttcatc cagtttgcgg gcggcaggaa gtgataacgg ggccgatgcc
     2581 gatcctgctc gacacgtcct tgagcctcat caggaaagtc catgttgcct gacctcacct
     2641 taaaactgct cctatctcga atggctcttt cacccagcgc gagcgttgcc ggacccgaca
     2701 aacgcgcatc aacttctgca gtgggtctta aggcagatgc gtcgaaagcc atatggcagt
     2761 ggctctccgc tggaacggca aaccgactgc tctcgtgcta ttcagacggt ctgctatctc
     2821 cgtattcggt agcttgactg acgaccgctg ccagggactc ttcgtatctt gggccggcct
     2881 taggtgcacg tcaacagaca attacggaat caggtaaatc ctgtgggaag cacgtctgac
     2941 acagtgatca ccaccaaggc cgccgccgta cggaaaacga aagcggtgcc tgctgtct
//
LOCUS       DASYWM010000234         4435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_347548, whole genome shotgun sequence.
ACCESSION   DASYWM010000234 DASYWM010000000
VERSION     DASYWM010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347548"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="VGR29_09410"
     CDS             <1..1761
                     /locus_tag="VGR29_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEV2073844.1"
                     /translation="AYDDANIGLDEGWQTRTDVFDREITVPFPPESRSSGIGAPAFHP
                     YVWYRRTFDVPAEDRSGRVLLHFGAVDYRAQVWVNGQFVAMHEGGHTPFSADITSALV
                     EGEQVVVLRAEDQPQDLSQPRGKQDWEPQPHKIWYHRTTGIWQPVWLEPVPAISIRNL
                     RWTPDLDRGILALHVGLSHQLSSPYQLRVRLSLRGAELAHHTSTFAHQDIRRDLSLES
                     ADITMSRERTLWSPSFPNLIDAEIEVLDGEEVIDAVTSYVGLRSCGFADGRFMLNGRP
                     TYLRLVLGQNYWPESHLAAPTEDALRREVELIKELGFNGVRIHQKVEDPRFLYWCDRL
                     GLMVWGEMANAYTFSADAVDRFTREWLEVIERDHSHPCIVAWVPFNESWGVPNMERDE
                     AQRNFVRALYYLTKAIDPSRPAIGNDGWEHMVGDVWGVHDYALDGDTLRERYGSFSAL
                     EQTMQEVRPHYRSIAFGDARRQGEPVMLTEFGGISYTPVAGERWHGYGTVGSQDEFLA
                     KYEELVGAILDSPAIAGFCYTQLTDTEQETNGLLTDDRLPKLDPAAIRAINKRPSKAV
                     PGEVISQIQYAAEVTTFEGP"
     gene            complement(1995..2987)
                     /locus_tag="VGR29_09415"
     CDS             complement(1995..2987)
                     /locus_tag="VGR29_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV2073845.1"
                     /translation="MIETNPFATLTEIAGGYCLPRSLHAVANLGVADALDEMPRTATD
                     LAASVSAHPGALGRVLRLLASHGVFEMQGDTVRHSPASRLLRTDHPQSMRAFAQMFGL
                     PIFWTTFGAMEHSVRTGLPAASEVFPEGFWTYLAGRPEEGQVFNSAMAAKAHGAIAGI
                     LTAYDFSGFGVIGDIGGGNGHLLHAILETVPNAKGVLFELPQVIEESASMASDRLVLQ
                     AGDFFRDALPSCDAYLLMEIIHDWGDEESIAILQAVRQASPPQAKLLVIETIVPDDPG
                     PDWSTMLDIHMLTLLGGKQRTQQEYEALFAQSGFRFQREIDTGAGISILEAEAI"
     gene            3075..3536
                     /locus_tag="VGR29_09420"
     CDS             3075..3536
                     /locus_tag="VGR29_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV2073846.1"
                     /translation="MANSPHDLPSSALLRTAYNILAARIFTSVVSQTDFNDLRPSHGN
                     AMEQLELEDGLRLTDLAGRAGITAQSMGELVDDLETKGYLERRPDPDDRRAKRIYLTD
                     RGRKNARAAKRATTDVEHDLAELLGEQRYTDLRRTLEDIISGFRSDDEGLI"
     gene            complement(3673..4374)
                     /locus_tag="VGR29_09425"
     CDS             complement(3673..4374)
                     /locus_tag="VGR29_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEV2073847.1"
                     /translation="MSATGSRWTGRMNMRVAIVSDVVCPWCRIGKANLMQAAEQWTRE
                     TGDQVGVALLPFLLDPVEPGVKEDFRERFTKRKEIPESQMEAMFERVTQVGAQQGLRF
                     NFDKVKVAVDTVPAHELMELTPREKQFGLMDAMMSAYFEEGRDIGEIDVLLSIAKDAG
                     LTDEELKEIEPALRSRKMEGQVRSTIAQMQQAGVQGVPFFILNDAVALSGAQPVEVFL
                     QAFEQAKDAQPAGAR"
BASE COUNT          863 a         1458 c         1285 g          829 t
ORIGIN      
        1 gcctatgacg acgccaacat cgggctagac gaaggctggc agacccgtac cgatgtcttc
       61 gaccgcgaga tcaccgtgcc attcccaccg gaatcccgct ccagcgggat cggcgccccc
      121 gccttccatc cctacgtctg gtaccgccgc accttcgatg tccccgccga ggaccgctcc
      181 ggtagggtgt tgctacactt cggcgcggtc gactaccggg cacaggtgtg ggtcaacggc
      241 cagttcgtgg ccatgcatga gggagggcat accccgttca gcgccgacat cacctcggct
      301 ctcgtcgagg gggagcaggt cgtcgtcctc cgcgccgaag accagccgca ggatctctcc
      361 cagccacgcg gcaagcagga ctgggaaccg cagccacaca agatctggta ccaccggaca
      421 accggtatct ggcagcccgt ctggctggaa ccggttcccg ccatatcgat ccgcaacctt
      481 cgctggacgc ccgatctcga ccggggtatt ctggcgctcc acgtcggcct cagccaccaa
      541 ctgtcgagcc cataccagct ccgtgtgcgg ctgtcgctgc ggggggctga gcttgcgcac
      601 catacatcca cctttgccca ccaggacatt cgccgcgacc tctccctgga atcggcggat
      661 atcaccatga gccgggagcg caccctgtgg tcgcccagct tcccgaacct gatcgatgcc
      721 gagatcgagg tgctggatgg cgaggaagtc atcgatgccg tgacatccta cgtcggcctt
      781 cgctcgtgcg ggtttgccga tggccggttc atgctcaacg gccgccccac ctatctccgg
      841 ctggtgctcg gccagaacta ctggccggag tcccatctcg cggcaccaac tgaggatgcc
      901 ctgcgacgag aggttgagct gatcaaggag ttgggcttca acggcgtgcg tatccaccag
      961 aaggtcgagg atccgcgctt cctctattgg tgcgaccgcc tcggcctgat ggtctggggg
     1021 gagatggcta atgcctacac attctcggcg gacgcggtcg accggttcac gcgcgaatgg
     1081 ctggaggtga tcgaacgcga ccacagccac ccctgcatcg tcgcctgggt gccgttcaac
     1141 gagagctggg gcgtcccgaa catggagcgg gacgaggccc agcgaaactt tgtgcgggcc
     1201 ctctattacc tgacgaaggc aattgatccc tcgcgtcctg cgatcggcaa cgacggctgg
     1261 gagcacatgg tcggggacgt ttggggagtc cacgactatg ccctcgacgg cgacactctt
     1321 cgcgagcggt acggatcgtt cagcgccctt gagcaaacga tgcaggaagt ccgcccccat
     1381 taccggtcga tcgccttcgg cgacgccagg cgacagggtg agccggtcat gctcaccgag
     1441 ttcggcggta tctcctacac gccagtcgcc ggtgagcgct ggcacgggta cggcaccgtc
     1501 ggctcgcagg atgagttcct ggcgaaatat gaggagcttg tcggggcgat cctcgactca
     1561 ccggccatcg ctggtttttg ctacacccag ctcaccgata ccgagcagga aaccaacggc
     1621 ctgctgaccg acgaccggct tcccaagctg gacccggccg ccattcgtgc gatcaacaag
     1681 cgaccgtcca aggcggtccc gggtgaggtc attagccaga tccagtacgc cgccgaagtg
     1741 acgacgttcg agggcccttg atgccgggct ggcggcaaac aagtggtgga tgattgaaca
     1801 caccgtcttc ttctgcgcgt tacctgcaac ctgccggtgt ggtgcaccgg gaagacgagg
     1861 aacgaccctc cccttctcgg ctgccgtccc cgaattggtc gcaggactgg cacgactcat
     1921 ccgagatgcc ttgaagttcg atccgtttga aacaccgcgc ccgcctggcc ggacattggc
     1981 aatcgacgac aaccctagat agcctccgcc tccaggatcg agatcccggc gccggtatcg
     2041 atctcacgct ggaaccgaaa gcccgactgg gcgaagagcg cttcatactc ctgctgcgta
     2101 cgttgtttgc cgccaagtag cgtaagcatg tgaatgtcca gcatcgtcga ccagtcggga
     2161 ccaggatcat cggggacgat cgtctcgatc accaacaact ttgcttgggg cggggaggcc
     2221 tgcctcaccg cctggagaat ggcgatggac tcttcatccc cccagtcatg gatgatttcc
     2281 atgagcagat acgcatcgca gctcggcagc gcatcccgga agaaatcgcc tgcctgaagg
     2341 accagtcggt cggacgccat gctggcagac tcctcgatca cctgtggcag ctcaaacagg
     2401 acacctttgg cattcggtac agtttcgagg attgcgtgca gcagatgacc attaccgccc
     2461 ccgatatcgc cgatgacacc gaatcccgag aaatcgtacg cggtcagaat tcctgcgatt
     2521 gcgccgtgcg ccttcgcagc cattgccgag ttgaacacct gaccctcttc cggccgtccg
     2581 gcaaggtagg tccagaaccc ctcagggaat acttcggatg ccgcgggcag accggtccgc
     2641 acggaatgct ccatggcccc aaaggttgtc cagaagatgg gcaggccgaa catctgggcg
     2701 aaggcgcgca tggactgtgg gtggtcggtc cgcaggagcc gcgatgcggg tgaatggcgc
     2761 accgtatcac cttgcatttc gaacacaccg tgtgacgcaa ggaggcgcag gacgcggcca
     2821 agcgcaccgg ggtgggcgct cacggacgcg gcaaggtccg tggcggttcg gggcatctcg
     2881 tccagtgcgt ccgcgacgcc caggttggcc accgcgtgaa gacttcgcgg caggcaatag
     2941 ccccctgcta tctcagtcaa cgtcgcgaac ggattagttt caatcacgat cacccctcct
     3001 tgcgtgctat actcgaaaaa tattcaggct tcctttatat acggtatcct tagtacttgc
     3061 aggtgtcaac ccccatggca aactctcctc atgaccttcc ctcaagcgcg ctgcttcgca
     3121 ccgcctataa catattggcg gcccgtatct tcacgtctgt cgtgtctcaa accgacttca
     3181 atgacctgcg cccgtcccac ggcaatgcta tggaacaact tgaacttgag gatgggcttc
     3241 gtctgaccga cctggctggg cgggcgggca tcaccgcgca gtcaatggga gaactcgtcg
     3301 acgacctgga gaccaagggc tatcttgagc gtcgaccaga cccggacgac cgccgcgcca
     3361 aacggatcta cttgaccgat agaggacgaa agaacgctcg tgcagccaag cgagcaacaa
     3421 ctgatgtgga gcatgacctg gcagagcttt tgggagagca acgttacaca gatttgcggc
     3481 gcacccttga ggacatcatc tccggctttc gcagcgatga cgaaggatta atttaaggaa
     3541 gagcccccgg taacagacaa gtgacgtgct ccttcgtctg caccggggga atgttcagtt
     3601 cgttgtgacg ttgcgagatg tagcgggctc tcaacctcat ggttcaaggt tggaggatcc
     3661 tgccatcaga cactacctgg cccccgcggg ctgtgcgtcc ttcgcctgct cgaacgcctg
     3721 caggaagacc tcgaccggct gggcgccgga aagcgccacc gcatcgttga ggatgaaaaa
     3781 gggcacgccc tgcacgcccg cctgctgcat ctgcgcgatc gtcgagcgca cctgaccctc
     3841 catcttccgg ctgcgtaagg cgggctcgat ctctttcagc tcctcgtccg tcagcccggc
     3901 gtccttcgcg atcgagagca gcacatcgat ctcgccaatg tccctgccct cctcgaaata
     3961 ggccgacatc atcgcgtcca tcagaccaaa ctgcttctca cgcggggtca gctccatcag
     4021 ctcgtgcgcc gggacggtgt caaccgccac cttgaccttg tcgaagttga agcgaaggcc
     4081 ctgctgcgcg cccacctggg tgacccgctc gaacatcgcc tccatctgtg actccggaat
     4141 ttccttccgc ttcgtgaacc gctcgcggaa atcttccttc acacccggct ccaccgggtc
     4201 cagcaggaac ggaagcagcg cgaccccgac ctggtcgcca gtctcccgcg tccattgctc
     4261 ggccgcctgc atcaggttcg ccttcccgat ccggcaccat gggcacacca catctgacac
     4321 aatcgcaaca cgcatattca ttcgtcctgt ccaccgcgac cctgtcgcgg acattccata
     4381 acccgggtgg tctaccactc agcagatcgc tttccaaagg gaagtatcgg ccacc
//
LOCUS       DASYWM010000235        14100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_479426, whole genome shotgun sequence.
ACCESSION   DASYWM010000235 DASYWM010000000
VERSION     DASYWM010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 14100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14100
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479426"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VGR29_09430"
     CDS             complement(<1..180)
                     /locus_tag="VGR29_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073848.1"
                     /translation="MSSVGQTLTSRAELALNPIFRKGILGATNNTIVARTVRRYGMKL
                     GASRFVAGETLDECLP"
     gene            complement(311..463)
                     /locus_tag="VGR29_09435"
     CDS             complement(311..463)
                     /locus_tag="VGR29_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073849.1"
                     /translation="MMLAQQHSREALATFERKRFAALSRELTKAGLGDVTVLRELMTD
                     GSAPVL"
     gene            complement(526..2007)
                     /locus_tag="VGR29_09440"
     CDS             complement(526..2007)
                     /locus_tag="VGR29_09440"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEV2073850.1"
                     /translation="MLSRLSFSIYDLRDSLWYRPALMTLGAIVVAFVMIWIDEQVLQG
                     SNLRTWWLFSGGVEGARGVLSAIAATMITVVTTAFSITMVTLQLSSSQYSPRILRGFT
                     GDPGNQLVLGIFIATFVYCLLVLRSVRSELEDQRTFVPALAISLALVLALVCIGSLIY
                     FLHHASRTIQASVILDKSANDTFGLIEDHFNVGNSERMSDRTRSLLDNLPVVTEVISE
                     RPGYLRGMDDSTLVALARKYDLLLTLHPHVGNYIFTGSPLATVQRFTTRELNEDDEHS
                     EEESVFIRLKERIAEVLSPNSADADKKEKDGNGRSEVEKISDAIRGTFTTGLERTLNE
                     DVLFGFQQLADIGLRALSPGVNDPTTAMFCIDWLGEGLIRVQDTNDRPTVAVDEDGTP
                     RVVYPPIPFEQFLTISFRHIRHYAAGDPFVSRHIIEVLESVYRSSTNEQARAAVAEGA
                     RRTVEAHAASDPLPTELERVRMAASWAYTEDSVPLEYEHTAQT"
     gene            2516..2686
                     /gene="rpmH"
                     /locus_tag="VGR29_09445"
     CDS             2516..2686
                     /gene="rpmH"
                     /locus_tag="VGR29_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV2073851.1"
                     /translation="MSTKRTYQPKRLKRIRKFGFMSRMSTKGGRHVLNARRRKGRHAL
                     TVAEEKKFTQNK"
     gene            2793..3242
                     /gene="rnpA"
                     /locus_tag="VGR29_09450"
     CDS             2793..3242
                     /gene="rnpA"
                     /locus_tag="VGR29_09450"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV2073852.1"
                     /translation="MVARKYRLREESDVGRVRSRGRAVAHGPLVVRFMPNALNPAQNR
                     YTVIAGKRCGKAVQRNRLKRLVREALRGYHPYLEPGHDIVVICRGTVEEMPALLDAQK
                     ALKNILDRARIFRPGSDVPEPGSAITTGWSEEDTGATKTTAPDEVSV"
     gene            3239..3448
                     /gene="yidD"
                     /locus_tag="VGR29_09455"
     CDS             3239..3448
                     /gene="yidD"
                     /locus_tag="VGR29_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV2073853.1"
                     /translation="MKRSALGMIRFYKSRVSPLLPPSCRFHPTCSEYAYEAVEKYGIL
                     KGGRLAIWRILRCNPFGGSGWDPVP"
     gene            3687..4880
                     /locus_tag="VGR29_09460"
     CDS             3687..4880
                     /locus_tag="VGR29_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEV2073854.1"
                     /translation="MLFDLPGLVALSADHMAQMVSTLVPGIILWDQYVDFVEWVLAQL
                     GSVFNSGGLAIIAFTIIIKTLLLPLTVKSIRSSKAMQELAPKIKELQKKHGADRQKAS
                     AETMALYQQYGVNPMAGCLPMLIQMPIFFGVYFGIREMSGSGAEEWANGFLWLDSLKE
                     ADPLHLLPILAGFFQFIQTRMMRPANQKITDPQQQIMNTMMTFMPLTVVLFGWGFDSG
                     PVLYWVVQAIYSVVQQWLITGWGSMGEWFPWLPELPEHRRLGYATPRDPNDLVVMSGE
                     GAAAQRSGFQGWLAKRMEEAQQNAQARQESLREQGKANGGKKAPANGTSDEVIETTAT
                     PTKQSSKRSGYQARVDAASKYRAAGSSKVVESSTASADAARTRVAGAKTTNPARPRKK
                     KKQRA"
     gene            4983..5822
                     /gene="jag"
                     /locus_tag="VGR29_09465"
     CDS             4983..5822
                     /gene="jag"
                     /locus_tag="VGR29_09465"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEV2073855.1"
                     /translation="MPKRTSVEIQAISVDEAVRLALEQLELTRDDVDIEILSEEGPAE
                     DAEALVRVTAKGQASQPVESSRPTREREERGRNRRRGGSGGNREQLGGRTSPGDRPSR
                     RGTPPPAAGVTRERVDAEHEQVAKEIVRDLLVRMGVEGDVVAVDNPSSVPVGPDEPPT
                     IFIDVLGRDLGMLIGRRGEHLSHLQYLVNTIVNRRLGDWNRVILDVEGYRSRREESLV
                     GLAERVARQVARSGRPIVLEPMPPNERRIIHLTLLSHPGVATQSSGEGSQRRITVEPA
                     RQG"
     gene            5985..7055
                     /locus_tag="VGR29_09470"
     CDS             5985..7055
                     /locus_tag="VGR29_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV2073856.1"
                     /translation="MTQDDRDRRDPQAPTRDTPASDATSGEKPGTASRRRDKVRAPFR
                     QQAERSPLQSSRTPDYLLIGHITADLMDNGEVVLGGTALYSAITAARLGARVAVITRG
                     VFGREVQGMNIPSLDEFAGDFQIIVQDADVPTTFVNEYLATRRVQTIKHWAGRIDLRG
                     LPPHWRNSKIVHLGPVADEIDPRHVPGMTTGFLGVTPQGWMRDWPRERGGRVREVPLR
                     LPADLIGRIDCAIVSDEEIFQAREVIERVGERRLSVVTRGPAGARIYYSNRSEDNERI
                     RPGASQFKTLEIPGVDVKVKSLTGAGDVFAAAFFMKAADKSVSAKDAGMYANAVAALS
                     LREIGAGTLPSPAEVEAILRDS"
     gene            complement(7237..7626)
                     /locus_tag="VGR29_09475"
     CDS             complement(7237..7626)
                     /locus_tag="VGR29_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2073857.1"
                     /translation="MFATSRSRPDSRGTPDGAVVLIVDDERHIVDLLEDLFHDEGYQV
                     RRAYDGMAALAAIDREMPDLVVADVMMPRLDGLSLYHRIRERSNSLPVILMSAAVTPR
                     AVDATFIPKPFDIDTLLDVVEEKLRGH"
     gene            7904..8533
                     /locus_tag="VGR29_09480"
     CDS             7904..8533
                     /locus_tag="VGR29_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2073858.1"
                     /translation="MPLTIRRVVGGPLETNAFLVTDTSSGDAIVIDAPPGVTEEVTST
                     ANSLGATVHQIVVTHGHWDHIQDLNALNQALDAPIFGHPDLRERIESPDPATSPEPIM
                     PVTMDGELDEGDRVTVGGHVFTVMHMPGHDPAHIILYSEDDGLVFGGDVLFPGGHGRT
                     DIPGSDQSAMNRTLRRFIDLPGEVRVFTGHGEETSLEAEREWIMRVPAE"
     gene            complement(9131..9346)
                     /locus_tag="VGR29_09485"
     CDS             complement(9131..9346)
                     /locus_tag="VGR29_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073859.1"
                     /translation="MEVQGTLEGRQDYAIHGQRYARLFFSLPDNPDAIQQCQLPDDAF
                     DADLKAGDRIVITFLLKTVMEIHKAPA"
     gene            complement(9352..9999)
                     /locus_tag="VGR29_09490"
     CDS             complement(9352..9999)
                     /locus_tag="VGR29_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2073860.1"
                     /translation="MTTPPDTNQSGPEEGRGPLIFCGMCGALNPATNFYCAACGTTLV
                     DAFHATEGLRVFERPDSASRLIEIVPSGTELEVADDPDAPEDFVRVRLQHGRLGYIRM
                     QDVDALANLSGAVHRTTPAGRPMPDINTNATGCISSTAALGSVVLVAMLGLLMIVIAM
                     RANTYDQGTLALVFCFGIGPLMIMTVAFYLYAKSREDRLADEDDEAALHQADIRE"
     gene            10150..11157
                     /locus_tag="VGR29_09495"
     CDS             10150..11157
                     /locus_tag="VGR29_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073861.1"
                     /translation="MTESTITRQCGNCGQAVSEGDVTCPHCDVLLAAYEAPAGATSGT
                     ASATTPVSMPAEPASAPIPPVTQSSSTYQRPVYTSPTTDTAGDLNANRPTPLSPTPAF
                     DRPRSSPVADAVERTRAAIDETAEAGDKPGTPNTETASTSTVARRERELLGMPATPVD
                     LSQGTQEALDRSHDARETPPASSTTTIRPRRHQSQERPASPAPQSTPMPGTPQQEVIP
                     TPTLEWPRPTPEERAATRQLPVIPSNLKNNENQRHQERYRAQPSTTGGRPFPNIRSVV
                     GFVIMLIIVMRFLSVAPFSGLIFIPIVIMFLVWLMTAIARTSGRKTTAMPKAGKHDRH
                     R"
     gene            complement(11187..11612)
                     /locus_tag="VGR29_09500"
     CDS             complement(11187..11612)
                     /locus_tag="VGR29_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV2073862.1"
                     /translation="MGKMTTGEIYTFLKNYAYTGHLATVREDGRPHVAAIWIVVDGTD
                     ILFTTFHSSVKGRNLQRTGYAALSVDDSTPPFTAVQLEGPVEMIRDQEEVRRWAGIIG
                     GRYMGEDRAEEFAARNGVEGEWLCRMRPLKLSGIAAITE"
     gene            11701..13098
                     /gene="mnmE"
                     /locus_tag="VGR29_09505"
     CDS             11701..13098
                     /gene="mnmE"
                     /locus_tag="VGR29_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV2073863.1"
                     /translation="MDNATIAAIATPTGEGGIGVVRISGSDAADVASRIFYRSSSSRT
                     IDLKSVPSHRLLYGSIVDPSTSNVIDEVLLAWMKAPNTYTREDIVEISCHGGPIPLRE
                     TLRVALAAGARHAERGEFTLRAYLNGRLDLTQAEAVLNVIGARTAESLRLAVDDLGGD
                     LSRRLEPANTALIRLLAYLDASADFPDDEIDLADIENGLTKAEGALAEVAAGSRAGML
                     YREGVQIALVGRPNVGKSSLLNALLRFDRSIVTPIAGTTRDVIAETVNLHGIPATLLD
                     TAGIVETDDPVEQIGVERSRKALGTAAAVALIVDGSLPITQGDLDIARALADRPPDEQ
                     TPVVLVVNKRDLLEQHAPDAVTRLLPDAAMTYVSATTGEGLSDLEQMLAQAVSQDVTG
                     GARPALINARQRAAIDRALEHIRDARSAHAAGIPQDLLATSVRAALHAVGEVTGENVD
                     EAVLTEIFSSFCIGK"
     gene            complement(13073..>14100)
                     /locus_tag="VGR29_09510"
     CDS             complement(13073..>14100)
                     /locus_tag="VGR29_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HEV2073864.1"
                     /translation="IGRTPLRLFRPEKDDPFVLRMGDQVRFERIGVEEFHRAEKGVGE
                     QEVGSATGDVEVLAPGMQTTVQDLGRYGYGRFGISPNGAADRTSLVAANRRIGNPDSA
                     AGLEITLTGPTLRFQRRTEIVLTGADLGAHLNGLPLPPGRTQGLMPGDELSFAGVQGR
                     GARAYLAVAGGIEVPVVMGSCSTDLTAGIGGHGGRALRAGDHLHIGSQRRSGPIGSTV
                     STPVDQLSRFDVMPGPQAERFDERAWESLLGHPFVVSTSSNRVGLRLEGPEIHPIDSA
                     DIISEGIVTGAIQITGEGQPIVMLPGHATIGGYTKIATVVEEDLDRLGQLRPGDEICF
                     TCRYKTS"
BASE COUNT         2849 a         4447 c         4235 g         2569 t
ORIGIN      
        1 cggcaggcac tcatcaagtg tttcgcctgc aacaaatcga gaggcaccga gcttcatgcc
       61 atatcgacgc acggttcggg cgacaattgt attgttcgtc gcaccgagaa tcccctttcg
      121 gaagatcgga ttcagcgcga gctcggcgcg ggaggtcagc gtttgaccaa cgctggacac
      181 gtccaatccg gtggtgctca tagacgttcg ccaatcgctt tggcctcaac gaaattgtgc
      241 aggtattctg gcccccctgc cttggagttc gtcccactca acttgatgcc ggcaaaggga
      301 tgtactcccc tcatagcacc ggtgcacttc cgtcggtcat gagttcccgc agtaccgtca
      361 cgtccccaag acccgctttg gtgagctccc ggctgagcgc agcgaaccgc ttccgttcga
      421 aggtcgccaa cgcttcccgg gaatgctgct gggccaacat catctacggc ctcgtcatga
      481 ataggggaga gccttctggc attcaggacc cagatgacgc gatcgttacg tttgcgccgt
      541 atgctcgtat tctaacggca ctgagtcctc cgtgtaagcc catgaggccg ccatgcggac
      601 ccgctcaagc tcggtcggca gcgggtcgct ggcggcatgc gcctcgaccg tcctccgcgc
      661 tccctctgca accgctgccc gggcctgctc gttcgtggag ctcctatata ccgactccag
      721 cacttcgatg atatgacgac tgacgaaggg atcgcccgca gcgtagtgac ggatgtggcg
      781 gaaggagatc gtcaggaatt gctcgaacgg gatcggggga tagaccacgc gcggcgttcc
      841 atcctcgtcg accgcaaccg tcgggcgatc attggtatcc tgcacccgga tcagtccctc
      901 accaagccag tcgatgcaga acatggccgt agtcgggtcg ttcacccccg gcgagagcgc
      961 ccgcagcccg atatccgcca gttgctggaa accgaagagc acatcctcgt tcagtgtccg
     1021 ctcaagcccg gtagtaaacg tgccacgtat cgcatcggag atcttttcca cctcgctccg
     1081 gccgttgcca tctttctcct tcttgtcggc atcggcagaa tttggggaaa gaacctccgc
     1141 aatccgctcc ttcaggcgaa tgaagacgct ctcctcctcc gaatgctcgt catcctcgtt
     1201 caactcgcgt gtcgtgaacc gctggaccgt ggccagtggc gacccggtga agatgtagtt
     1261 cccaacatgg ggatgcaacg tcagcagcag gtcgtacttc cgggccagtg cgaccagcgt
     1321 ggaatcgtcc ataccgcgca ggtaaccggg ccgctcggaa attacctccg tcaccacagg
     1381 caaattgtcc aacagtgagc gggtacgatc cgacatccgc tcactgttcc ccacgttaaa
     1441 gtgatcctca attagcccaa aggtatcgtt cgcggatttg tcgaggatga cggatgcctg
     1501 gatggtccgg ctggcatgat gcaggaaata gatgagggag ccaatgcaga caagcgccaa
     1561 cacgagcgcc agcgagatcg ccagggcggg aacaaaggtc cgctgatcct ccaactcgga
     1621 ccgcacggag cgcaggacca acaggcagta gacgaaggtg gcgataaaga taccgagaac
     1681 gagctggttt ccggggtctc cagtaaaccc gcgcaggatt cgcggggaat actggctgga
     1741 gctcagctgc aaggtgacca tcgtgatcga gaaggctgtc gtcaccacgg ttatcatcgt
     1801 tgcggcaatc gcggaaagga cgccgcgggc cccctccaca ccgccgctga agagccacca
     1861 agtgcgcagg ttcgagccct gcaggacctg ctcatcgatc caaatcatca cgaaggccac
     1921 cacaatcgct ccaagcgtca tgagcgccgg gcgataccag aggctatccc tgaggtcata
     1981 gatggagaac gagagacggg acagcatgga cggccctccg gcgtcatggt tggtgcacag
     2041 ggttccccgg tgacggcaag atcagacccg atgatacaag aacatgatca aaacccacgg
     2101 tgagaagtgc cacaccttgg cgccaaggtg tagcgcgtgt tcgcctgttt ccgacaacgc
     2161 cagcgtgcga ccttgcggca gattcacctg aatatcccat agaaacgctc gcctccgacc
     2221 ggtcccgtat tccgtgatcc ctcgtggacg ctcacctgta tgcacccgga tagtggttga
     2281 cggggcaacg gggcgcggcc tatactcaac cgtctcgcac accgtggaac ggtgatggcg
     2341 tgccatcggc gtgacacaat gcgcgtcttc ttcggcccgg tacatcgcta ccgcttcccg
     2401 acccaggagt gcatcctcta gcaacacgcg cgtggctttc gttgcctgtc gacggccgcg
     2461 cgatgcgcca ggaccgaggt ccggcgccag acacaacgat ttaaggagta tttgcgtgtc
     2521 caccaagcgc acctatcagc caaagcgcct caagcgcatt cgcaagttcg gtttcatgag
     2581 ccgcatgtcc accaagggcg gccgccatgt gctcaacgct cgccgccgca agggacgtca
     2641 cgcgctgacc gttgccgaag agaagaagtt tacccaaaac aagtaatcgt taccccgatg
     2701 gtaacgaggc gaggacggca acgacgcaga tgcgccatgc cgatcctcgc ttttttcttg
     2761 tacggcctcg tgcaaggtga ggacatgctt ccttggtagc gcgcaagtac cggctccgtg
     2821 aagaaagcga tgtagggcga gtgcgctcac gtggacgagc ggtcgcgcac ggcccgcttg
     2881 tggtccgctt catgcccaat gcgctcaatc cggcccaaaa ccgctacacg gtgattgctg
     2941 gaaagcgctg cggcaaggcc gtccaacgca accggctcaa acgcctggtg cgagaggccc
     3001 tgcgcgggta tcacccgtac ctggagccgg gacacgacat tgtggtcatc tgccggggta
     3061 cggtggagga gatgccggcg ctcctcgatg cccagaaggc cctgaaaaac atccttgacc
     3121 gcgcgaggat cttccgtccc ggcagtgacg taccagagcc gggttccgcc attaccaccg
     3181 ggtggtcgga ggaggacacg ggggcaacga aaacgacggc acccgacgag gtatcggtat
     3241 gaagcgctct gcccttggca tgattcgctt ctacaaaagc cgcgtctcgc cgctgcttcc
     3301 gccctcctgc cggtttcacc cgacctgttc ggaatatgcc tacgaggccg tcgagaagta
     3361 cggtattctt aagggcggtc ggctggctat ctggcgcatt ctgcgctgca atccgtttgg
     3421 cggaagtggc tgggatccgg tcccgtgacc ctgtattgac gcacgttcac gcccgcaggg
     3481 aagcacagct gttcgtgata gactgccgcc gggtcaaacg caaaagcgtt tggcatatcg
     3541 gcaagagtgg aaccggggcg atgtccgacc gtatacatac actggcatgt cgcaaccgtc
     3601 gcgtaacctg aacaacgtat caatcccgca acgctgcaca atgcagcgct cggaacccgc
     3661 ccgcttgcgg gaggaggccc atcctcgtgc tcttcgatct ccccggtctc gttgccctct
     3721 ccgccgatca catggcgcag atggtatcaa cccttgttcc cggcatcatt ctttgggacc
     3781 agtacgttga cttcgtggaa tgggtgctcg cccagctggg cagtgtgttc aacagcggcg
     3841 gcctcgcgat catcgccttc accatcatca tcaagacgct cctcctgcca ctaacggtga
     3901 agtcgatccg ctccagcaag gcaatgcagg agctggctcc gaagatcaag gagcttcaga
     3961 aaaagcacgg tgcggaccgg cagaaagcct ccgcggaaac aatggcgctc taccagcaat
     4021 atggcgtcaa ccccatggcg ggctgtttgc cgatgctgat ccagatgccg atcttctttg
     4081 gcgtctactt cggtatccgg gagatgtccg gaagcggtgc cgaagagtgg gccaatggct
     4141 tcctctggct cgactccctc aaggaggccg atccgcttca tctgctcccg attctcgccg
     4201 gattcttcca gttcatccag acgcgcatga tgcgcccggc caaccagaag atcaccgatc
     4261 cgcagcagca gatcatgaac acgatgatga cgttcatgcc gctgaccgtg gtgctcttcg
     4321 gttggggatt cgactccggc ccggtgctct actgggtcgt ccaggcgatc tactcggtcg
     4381 tgcagcagtg gctgatcacc ggttggggca gcatgggcga atggttccca tggttgccgg
     4441 aactgccaga gcatcgacgg ctcggctacg caactccgcg tgaccccaac gaccttgtgg
     4501 tgatgtcggg tgagggtgca gcagcacagc ggtccgggtt ccagggatgg ctcgcgaaga
     4561 gaatggagga ggcccagcag aacgcccagg cccggcagga gtcgctccgc gagcagggca
     4621 aggccaatgg tggcaagaag gctccggcaa acggtacctc cgatgaggtc atcgagacca
     4681 cagccacgcc gacaaaacag agctccaaac gatccggata ccaggctcgc gtggacgcag
     4741 cctcgaaata tcgcgcagcc ggctcaagca aggtcgtgga atcatccacc gcgagcgccg
     4801 atgccgcccg cacccgtgtc gccggagcga agacgaccaa cccggcccgg ccacggaaaa
     4861 agaagaagca acgcgcctga tcgcctgccg caggggacat cgccgccggt gatcggccca
     4921 ctccaaaccg gaaacttgct atcgcaacgc cgcatctcgc gaatcgccac aaggaagaga
     4981 cgatgccgaa acgcacaagt gttgagatcc aggccatctc ggtggacgag gccgttcgtc
     5041 tggccctgga gcaactcgag ctcacccggg acgatgtcga tatcgagatc ctctccgagg
     5101 agggcccggc tgaggacgcg gaagcgctgg tgcgcgtgac cgcgaagggg caggcgtccc
     5161 aacccgtcga gagcagcagg cctacccggg agcgggagga gcgcggtcgc aatcgtcgcc
     5221 gtggaggcag tggtggcaac agggagcagc tgggtgggcg gacctcgcct ggcgatcgcc
     5281 cctcccgtcg cggcacgccg ccgcctgcag ccggggttac tcgagaacgg gtcgatgccg
     5341 agcacgagca ggttgccaag gagatcgtcc gggacctgtt ggtgcgcatg ggtgtcgagg
     5401 gcgatgtggt ggcggtcgac aatccatcat ccgtaccggt tggtcccgat gagccgccga
     5461 cgatattcat cgacgtgctc ggccgcgacc tcggtatgct gatcggccgc cgtggggagc
     5521 acctctccca cctgcaatac ctcgtcaata cgatcgtcaa ccgcaggctg ggcgactgga
     5581 accgggtgat cctcgatgtt gagggctacc gttcccggcg cgaggaatcg ctggtcgggc
     5641 tggcagaacg tgtagcccgg caggtagccc ggagcggtcg gcccatcgtg ctggagccga
     5701 tgccgccgaa cgagcgccgg atcatccacc tcaccctcct ttcgcatcca ggtgtggcca
     5761 cccagagcag cggtgaaggt tcccaacggc gcatcaccgt tgaaccagcg cgccaggggt
     5821 aacatcgcac tgccttgacc gaatgcaccc gggcggcaca agagttgacg gggcacaggt
     5881 ggcgcagccc gtcacgccat agactcctgt ccgagcgaga cattgcattg aaggggcttg
     5941 ggcggtatgc ccaggcccat tccatcagag aaacggacaa cttcatgacg caggacgatc
     6001 gggatcggcg cgatccacaa gcaccgacac gcgacacgcc ggcatccgat gcgacctcag
     6061 gggagaagcc gggcaccgcg tcccggcgtc gggacaaggt ccgcgcaccc tttcgccagc
     6121 aggcggaacg atccccgcta caaagctccc ggacaccgga ttacctgttg atcggtcata
     6181 ttacggctga cctgatggac aacggcgagg tggtgctggg cggcacggcg ctctactccg
     6241 ccatcaccgc ggcccggctg ggcgcacgcg tggcggtgat cacccgtggt gtcttcggtc
     6301 gcgaggtgca gggcatgaat atccccagcc tcgacgagtt cgccggcgat ttccagatca
     6361 tcgtccagga tgccgatgtc ccgaccacct tcgtgaacga gtacctggca acgcgtcgcg
     6421 tccagacgat caagcactgg gcaggtcgta tcgacctgcg gggacttcca ccccactggc
     6481 gcaactcgaa aatcgtccat cttgggccgg tcgctgatga gatcgatccg cggcatgtcc
     6541 ccgggatgac gaccggcttt ctcggcgtca cgccacaggg gtggatgcgc gactggccac
     6601 gcgagcgtgg cggcagggtg cgggaggtcc cccttcggct gccagcggac ctgattggcc
     6661 gcatcgactg cgcgatcgtg agcgacgaag agatcttcca ggcgcgtgag gtgatcgagc
     6721 gggtggggga gcgacggttg agcgtcgtca cccggggtcc cgccggcgcc cgcatctatt
     6781 acagcaaccg gtctgaggac aacgagcgca ttcgccctgg cgcctcccag ttcaaaaccc
     6841 tggagattcc gggcgtcgac gtgaaggtga agagcctcac cggtgccggc gatgtcttcg
     6901 cggcggcatt cttcatgaag gcggcagaca agtcggtctc ggcgaaggat gcggggatgt
     6961 acgccaacgc ggttgcggca ttgagccttc gtgaaatcgg agcgggtacc ctcccttccc
     7021 ctgctgaggt cgaggcgatc ctgcgcgact cctgagctcg ctgtaccagc tcacgacccg
     7081 gcgggtgact gctcattgcc gggagcgggc caaagcgcgc cgtatcaggc gatgcagcgc
     7141 gactccccct cgtcgatggg tcttggtcct ggcctttgaa gacgtgacct tgccgtagtg
     7201 gtcttccggc tacgttgcca gcctggcctc cggaggtcag tgtcctcgca gtttctcttc
     7261 aaccacgtcg agcagtgtat cgatatcaaa gggctttggg atgaacgtgg cgtccaccgc
     7321 gcgaggggtg accgcggcgc tcatcaggat tactgggagg ctgttactgc gctcccggat
     7381 ccgatggtag agagacaggc catcgagccg gggcatcatg acatccgcga ccacaagatc
     7441 gggcatttcc cgatcgatcg ccgccaatgc cgccatacca tcgtaggcgc gtcgaacctg
     7501 atagccttcg tcgtggaaca aatcttccaa tagatcgaca atatgccgct cgtcgtccac
     7561 gatcaggacg accgcgccat cgggtgtgcc tcgggagtct ggtcgtgagc gtgatgttgc
     7621 gaacatggtc atatcattca acatggatag cggttccgga tcgttttcaa cagagcaggg
     7681 agatacaaga atcactccct caacaactgt tcgggagtaa gcatcctgtc acgaacggat
     7741 ggtccgttcg cctgctgtct cgctgctagc acattccgta ccgtacccgt cccggatcga
     7801 taccatcgaa gagtttttgg ctccagatag ggagaagatg agccgggaca gtatcccgct
     7861 cagagcgctt tttggcacaa gccaatatgt agggaggatt tgcatgcctt tgactattcg
     7921 ccgtgtcgtc ggcggaccgc ttgaaaccaa tgcctttttg gtcacggata cgtcgagtgg
     7981 cgacgccatt gtcattgatg cccctcctgg agtgacggaa gaggtcacca gcacggccaa
     8041 ctcactcggt gccacggtgc atcagatcgt tgtgacccat ggccactggg accacatcca
     8101 agatctcaac gccttgaacc aggcactgga tgcgccgatc ttcggccatc ctgatctacg
     8161 ggagaggatc gagtctccgg accccgccac gagtccggaa ccgatcatgc ccgtgacgat
     8221 ggatggagag ctcgatgaag gcgatcgtgt caccgttggt ggccacgtct ttacggtgat
     8281 gcatatgccc ggtcatgacc ctgcccacat cattctctac agtgaggacg acggcctcgt
     8341 tttcggcggg gatgtcctgt tcccgggtgg acatgggcgg accgatatcc cgggttccga
     8401 ccagagcgcc atgaaccgca cactgcggcg cttcatcgac ctgccaggag aggtgcgcgt
     8461 tttcaccggg cacggcgagg agacatcgct cgaggctgag cgcgaatgga ttatgcgggt
     8521 gccggcagag tagcgagact aaaaagtacg gaagcagact atcgtgcgtt cggctaaatg
     8581 acaggggtac aactccagca cagttgccac tgtggtcacc cacgttgtgc aattgctgac
     8641 cgatgccatt ctctttcggg acggcgtcaa ggatttgcca acgaaaaagt ctgcgtatcg
     8701 gtaggaatcg gggaagggga gattacggcc ttcgctgatt cctgccgctg ctcctggttc
     8761 atggagactc ttccatcagg tctgaggcac ccggagggcg tggccaggaa tcgaaaaccc
     8821 aatcgttcgc cgcccaggcg gagattgacc agccctaata gcaatggatg acggacctgc
     8881 gtaggaggtc aggctccctg tactagacca ggctggagcg aatgctgtct gttaagtgag
     8941 agtcgtgaaa gataaaaccc ggcgcactgg ctctttcacg ctatcgacca tatatcgctt
     9001 catccgggcc tctccagact gagcggtaac tatggggagc agggcgccgt gcactcaccg
     9061 agacaatcaa ctgaattact gggagtctgg attgtccgga ttcgtatcgt cttgacgttc
     9121 tggcagcctg ctatgcgggt gccttgtgga tctccatcac ggtctttagg aggaaggtga
     9181 taacaatccg atcgccggcc ttcaggtcgg cgtcaaaggc atcatccggc agctggcact
     9241 gctggatcgc atccgggttg tccggcaacg aaaagaagag ccgtgcatag cgctgcccgt
     9301 gaattgcgta atcctggcgt ccttccagcg ttccctgaac ctccatgatc gctactccct
     9361 gatatccgcc tgatgcagcg ccgcctcgtc atcctcatcg gccaggcggt cctcccgact
     9421 cttcgcgtag aggtagaacg ctaccgtcat gatcatcagg ggaccgatgc caaagcagaa
     9481 gacgagcgca agcgtgccct ggtcataggt gttggcccgc atggcaatga cgatcatcag
     9541 cagacccagc atcgccacaa gcacaacgga accaagcgca gccgtcgagg agatacaccc
     9601 ggtagcattt gtgttgatat ccggcatcgg ccgtccggcg ggcgtcgttc gatgcaccgc
     9661 gccggagaga ttcgcgagtg catcgacatc ctgcatgcgg atatacccca ggcgtccatg
     9721 ctggaggcga acacgcacga agtcctctgg cgcgtccggg tcgtcagcca cctccagctc
     9781 ggtccctgag ggaacgatct cgatcaggcg cgaggcggaa tcgggccgct cgaagacgcg
     9841 aagaccctcg gtcgcgtgga aggcatctac caatgtggtg ccgcatgcgg cacagtagaa
     9901 gttcgtcgcc gggttgagcg cgccgcacat gccacaaaaa atcagcggcc cgcggccttc
     9961 ctcaggcccg gattgattcg tgtcgggagg agttgtcacg caagaagtcc tgtaccgcgg
    10021 ccgatggggt tcatcgtcag gaagctggtc aatggaaccg gaaccgaaag cacccggccg
    10081 tcgtcattcc gtcacatgca tctgtatatc ataggtgcag gcgaccggcg gtcgattcag
    10141 gatgaagcca tgacggagag cacgatcacc aggcaatgcg gcaactgcgg tcaggcagta
    10201 agtgaagggg acgtcacctg cccgcactgc gatgtgctgc tggccgccta tgaggctccc
    10261 gctggcgcca catccggaac tgcgtccgca accaccccgg tatcaatgcc agcagaaccg
    10321 gcatccgccc ccatccctcc ggtaacccaa tcttcctcaa catatcaacg accggtgtac
    10381 acgagcccca ccactgatac ggcgggtgac ctgaatgcga accggccgac accactctcc
    10441 ccgacaccgg cattcgatcg tccccggtcc agcccggtgg ctgacgctgt ggagcggacc
    10501 cgggcagcga ttgatgagac agctgaggcg ggtgacaagc ctggtactcc gaacaccgaa
    10561 accgcgtcga catccacggt cgcccgaaga gaacgagagc ttctcggcat gccggcaacg
    10621 ccggtcgatc tctcccaggg aactcaggaa gccctggacc ggtctcacga tgcgagggaa
    10681 acacccccgg cctccagcac gaccacgatc cggccaaggc gccaccagtc ccaggagagg
    10741 ccagcatcac ctgcgcctca gtcaacccca atgccgggca cgccgcaaca agaagtgatc
    10801 ccgacgccaa cgcttgagtg gccccggccg actcccgaag agcgagctgc aacgcgacaa
    10861 ctccctgtca tcccctccaa tctgaagaac aatgagaacc aacggcatca agaaaggtat
    10921 agggcccagc catccacaac aggcggcagg ccatttccca acatcaggtc ggtcgtcggt
    10981 ttcgtgatca tgctcatcat tgtgatgcga ttcctgtccg tagctccctt ctcgggactg
    11041 attttcattc ccatcgtcat catgttcctc gtctggctca tgaccgcaat cgcgagaacc
    11101 agcgggcgca agacgaccgc gatgccaaag gcagggaagc acgatcgcca caggtagtag
    11161 cggaaaccgg gatgccggag accggcctac tccgtgatcg cggcaatgcc cgagagcttc
    11221 agcgggcgca tccggcagag ccattcgccc tccactccgt tacgcgccgc gaactcttcc
    11281 gcacgatcct cacccatgta gcggccgcca atgatcccgg cccaccggcg tacctcctcc
    11341 tggtccctga tcatctcaac cggaccttcc agctgaaccg cggtaaacgg cggcgtgctg
    11401 tcatcgacgg agagcgcagc gtatccggtc cgttgcagat tcctgccctt gaccgagcta
    11461 tggaaggtcg taaagagaat atcggtgccg tcaacgacga tccagatcgc cgcgacgtgg
    11521 ggccgcccat cctctcgaac cgtcgccagg tgccccgtgt atgcataatt cttcaggaac
    11581 gtgtagatct ccccggttgt cattttgccc atcacgcttc accctttccg gggacgccca
    11641 cggcggcgcc ctcattgata tcgattcccc ctcgtcgatc accgcaggat acgcttctct
    11701 atggataacg ctaccatcgc cgccatcgcg accccgacgg gagaaggggg tattggcgtc
    11761 gtccgcatca gcggcagtga tgccgccgat gtcgcctcgc gcatctttta ccgttcctcc
    11821 agcagccgca ccatcgatct caagagcgtt ccttcccatc gccttcttta cggcagcatc
    11881 gtagacccct ctaccagcaa tgttatcgat gaggtgctgc tcgcgtggat gaaggcgccc
    11941 aatacataca cgcgggagga tattgtcgag atctcctgcc acggcggccc gattcccctg
    12001 agggaaacgc tccgtgtggc cctggcggcc ggtgcgcggc acgccgagcg gggggagttc
    12061 acccttcggg cctatctcaa cggtcggctg gacctcaccc aagcagaagc ggtgctcaac
    12121 gtgatcggcg cgaggaccgc cgaaagtctc cgcctcgccg tggacgacct tggcggtgat
    12181 ctctcccggc gccttgagcc agcaaacacg gcacttatcc gcctgctcgc atatctcgat
    12241 gcatcagctg acttccccga cgacgagatc gacctggctg atatcgagaa tgggctgacc
    12301 aaggcggaag gggcacttgc cgaggtcgcc gcaggatcaa gggccggtat gctctatcgg
    12361 gaaggtgtcc agatcgccct cgtagggcgg cccaacgttg gcaagagcag cctgctcaac
    12421 gccctcctgc ggttcgaccg ctcaatcgtt actccgatcg caggaaccac gcgcgacgtg
    12481 attgccgaaa cggtcaactt acacggtatc cctgccacac tgctcgatac tgcggggatt
    12541 gtcgaaaccg atgatcccgt cgaacagatt ggggtggagc gaagccgtaa ggcgcttggg
    12601 accgcggcgg cagtggccct catcgtcgac gggtcactcc cgatcaccca gggcgacctg
    12661 gatatcgcgc gagccctcgc ggacaggcca cccgacgagc agacacccgt ggtcctggtt
    12721 gtcaacaagc gggatctact ggagcagcac gcccccgatg ctgtgacccg gctcttgccc
    12781 gatgccgcta tgacctatgt ctccgcgacg acaggcgagg ggctctcgga tctggagcaa
    12841 atgctggccc aggcggtctc acaggatgtc accggagggg cccggccagc attgatcaac
    12901 gcgcgtcagc gagcggcgat cgaccgggca ctggagcata ttcgggatgc aagatcagct
    12961 catgcggcgg gtatccccca ggacctcctt gcgacctccg tacgggcagc actgcatgcg
    13021 gtcggggagg tcacaggcga gaatgtcgac gaggcggtac tgacggaaat cttcagctcg
    13081 ttttgtatcg gcaagtaaag cagatctcat cacccggcct caattgcccg agccgatcga
    13141 gatcctcctc gaccacggtg gcgatcttcg tatacccgcc gatggtggcg tgcccaggga
    13201 gcatgacgat tggctgtcct tccccagtga tctggattgc accggtgacg atcccttcgg
    13261 agatgatgtc agccgaatcg atgggatgta tctcgggccc ctccaaccgg aggccaaccc
    13321 ggttcgagct ggtagagaca acaaagggat gtccaaggag cgactcccat gccctctcat
    13381 cgaagcgctc tgcctgggga ccgggcatca catcgaagcg gctcagctgg tctaccggtg
    13441 tcgacacagt gcttccgatc ggcccgcttc gccgctgtga tccaatgtgc aagtggtctc
    13501 cggcgcgtag tgcgcgacca ccatgtcccc cgatacctgc ggtcaggtcg gtcgagcagc
    13561 ttcccatgac gaccggcacc tcgattccgc ccgcgacagc aagataggcg cgtgcgcctc
    13621 ggccctggac tcctgcgaaa ctgagctcat cacccggcat caggccctgg gtgcggcccg
    13681 ggggaagcgg aaggccgttc aggtgcgcac cgagatcggc accggtaagc acgatctccg
    13741 tgcgccgctg gaaacggagc gttggaccgg tcagcgtgat ctccagtccg gcggcactat
    13801 ccggattccc gatccggcgg ttggcggcaa ccaggctcgt gcggtccgcc gcgccgtttg
    13861 gagagatacc aaacctgccg taaccgtacc ggccaagatc ctgcacggtg gtctgcatgc
    13921 cgggtgcgag aacctcgacg tcgccggtcg cgctcccgac ctcctgctcg cctacaccct
    13981 tctccgcccg gtgaaactct tctaccccga tacgctcgaa tcgcacctga tcgcccatac
    14041 gaaggacgaa gggatcatcc ttctccgggc gaaacaaccg taggggtgtt cgcccgatga
//
LOCUS       DASYWM010000236        42797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_366911, whole genome shotgun sequence.
ACCESSION   DASYWM010000236 DASYWM010000000
VERSION     DASYWM010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 42797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42797
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366911"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..986)
                     /locus_tag="VGR29_09515"
     CDS             complement(78..986)
                     /locus_tag="VGR29_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2073865.1"
                     /translation="MSNNSPSSPSTNSAPLVLNERPDIGAVPKLLTAFVGRDRELTAV
                     VDLLRRPDVRLVTVTGTGGVGKTRLALRAAEDSHDTFDRVVFIPLAAVRDPVMLLPTV
                     AHYLNVPDTPDTPLLSRPRALLSNQQLLLVLDVVEHLLDAVPAVAELLAACPRLTILC
                     TCRTNLNVSGEHLLPLAPLPGEEARALFIERVRALVPGFTATDEVADVIDAICAQLDG
                     LPLAIELAASRVPMLPPRALLARLEHRLDLLTGGPRDAPPRLHAMRDAISWSHDLLPG
                     AAGALSTPRGLHWRVHPASSRGCGGQ"
     gene            complement(1242..2360)
                     /locus_tag="VGR29_09520"
     CDS             complement(1242..2360)
                     /locus_tag="VGR29_09520"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEV2073866.1"
                     /translation="MMTANTATLSTESTGRRRWVRWLALLLLLAVILLIVLHADLRAL
                     AVATWREMLSIPPAYLVLIVTFKVMQAALSSLSWRNALNWTWPHANLSYRFVVGIDQG
                     QDIVNAVVPSRAGTWAMLGIIGLAIPGARAPKLLTIWGVQNLAFILFASVSYTLVAVG
                     LPQQPQERQGPIERVSSFASAQPVLTGVVAGFIVILLIVAVIYGRRKLDQVRQQVREG
                     LAILGSPARYVRLMFLPSLASYLFRCGAYVVMLSAFGIPVTIWTVALALGSNALAGTV
                     RFTPGGLGTTQAIDIVALSAYAAPEVITAYSLSEIAITALVNTAVSIVALLSVNGWHG
                     NRAVFGHLRRGEFTTGLHSVGARQRALRARPLRQRRRP"
     gene            complement(2760..3317)
                     /locus_tag="VGR29_09525"
     CDS             complement(2760..3317)
                     /locus_tag="VGR29_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073867.1"
                     /translation="MTIHRSTTPLSRRSAVTGLATGAILLTGVATPSHAAQEASPVAE
                     RAPWHGHPVVGTWQWASNPVHPSSAVIADDGAYVEYDPLLGVGIGLWRATGEQTADVV
                     VTFQQVARAWGVFASDAVPEAPAFRSDRVVDLVTMRVMIEVDRAGNALTATGSITAHD
                     ANGKVVSTLDQYTGSAERLQADTST"
     gene            3316..3618
                     /locus_tag="VGR29_09530"
     CDS             3316..3618
                     /locus_tag="VGR29_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073868.1"
                     /translation="MADTVETRCTIGPGGFLDNGVTIARNDASVTSIRGRTVDQPYSA
                     RFALDADRVALLFTRYRALTRRNGGPGHAIVGNVPGRAYPDRRHILVGAANVDPHL"
     gene            3953..4735
                     /locus_tag="VGR29_09535"
     CDS             3953..4735
                     /locus_tag="VGR29_09535"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEV2073869.1"
                     /translation="MTQENHLQQHIETIASWMIEARHIVAFTGAGISTDSGIPDYRGP
                     GGVWTRRDAGLPPPQWRVAPNQVQPNASHLAFVDLQRLGKLQFLITQNTDNLHRQSGI
                     RPALLAELHGNGQLMRCLDCDRLHPRQDVGWDTRRWGPGYRTQNPVTGQPACPVCGGR
                     LISSVVNFGDPLPRHELTLADHHARSCDLLLVLGSSLVVEPAASLVGRAIRSRARVVL
                     VNRGETPYDEAVKLRLWSGISEVVPPAVERVKGTLGEQPERS"
     gene            4919..5944
                     /gene="pdxA"
                     /locus_tag="VGR29_09540"
     CDS             4919..5944
                     /gene="pdxA"
                     /locus_tag="VGR29_09540"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEV2073870.1"
                     /translation="MPGTTRPLLAITMGDPAGIGPEVVLKALAHPEVFARARPLVVGD
                     RSTLERAAHWVEATDLRYDVVTRVEDGQYGPGVVTLMDLQTSPSGGYAVGQVSADAGR
                     AAVDFVFRACDLAMEGKVDAVVTAPLNKAAMHAAGFTYPGHTELLAERTGAERVSMLL
                     VGPSLRVVHVSTHVALTEAIRRVTRQRVEDVIDIARDSCRALGIEHPRIAVAGLNPHA
                     SEGGLFGDEESREVIPAIEAARARGLNVSDPQPPDTVFLRATKGEWDIVVAMYHDQGH
                     IPMKLLAFDSGVNVSIGLPILRTSVDHGTAFDIAGLGQASENSMLAAIDVAVQMAGSR
                     SASGLAT"
     gene            5966..7189
                     /locus_tag="VGR29_09545"
     CDS             5966..7189
                     /locus_tag="VGR29_09545"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEV2073871.1"
                     /translation="MTQLAVLADDLTGGADAGAAFASVGLATSIAFASDADDCDVLVV
                     CTNSRGIDRTTAADLNRRAASAFITRPVSRRPRWVYKKLDSALRGHPRDELLAVMDGL
                     GESRALVAPALPAEARTTVDGRQFLDGSRLEATSLGSARNSSDLASLFGSGGDVPVHA
                     LDLKTIRGGQGEIERVLGQMGPGLVIADAETDADLMLIARASLGSDLRLLAGSAGLAG
                     QLARVLAPAPVSHNEITHRPANPILVVAGSLHQATAAQVEILQGAGLPIVRPVDVLLD
                     ESVRAIERTVDEVAMHLLAGRSVVVATAGLPSSQPGSTSVVSRLADIVAAPTVCRTIG
                     GLVLTGGETAAGILARLEAIELRLMGEIRPAMPWGILRSSHIRAVPIATKAGSFGDDD
                     ALLACVEHLTGTRLR"
     gene            7745..7954
                     /locus_tag="VGR29_09550"
     CDS             7745..7954
                     /locus_tag="VGR29_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073872.1"
                     /translation="MQAVGIVILLTVFALAVIPLTIAYYLWLARTVLPRVAAALPPRS
                     ARMVLAMCIALPWFVVALAVWWFRG"
     gene            8120..8449
                     /locus_tag="VGR29_09555"
     CDS             8120..8449
                     /locus_tag="VGR29_09555"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEV2073873.1"
                     /translation="MLFYCEFTWFPGTTRMQAARRVVQQHDAGANHPERIRGWYNLAG
                     GGAGFLIVEYDNIKELTAFLQPYMDLMSFDVRAIGELNYEERIEQLRGLGGQPDTEEP
                     PARMDYA"
     gene            complement(9608..9859)
                     /locus_tag="VGR29_09560"
     CDS             complement(9608..9859)
                     /locus_tag="VGR29_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073874.1"
                     /translation="MTGQRQFSESERMEIEEGIERAFVFVQDVLDDPSVLDQLPERVT
                     IALTPMAEKDASKSYVTETCHFTVSVDQPDSAHRHTHTA"
     gene            complement(10227..11333)
                     /locus_tag="VGR29_09565"
     CDS             complement(10227..11333)
                     /locus_tag="VGR29_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV2073875.1"
                     /translation="MTSGTVILLTGVLCAMACALLGTFLILRRMAMMTDAISHAILPG
                     LVAGYWLANSPNLLVGALGATGAAILTVSLVEALQRTRRIEGGEAMGIVFPAMFALGT
                     VLVSRFFANVHLDTDAILYGNIEFSFFDHLVIGGRDLGPQSLWIMGTLLVINLGFLFL
                     FYKELKLTTFDPGLAATIGFSPVLVHYVLMLVLSITTVGAFTAVGAILVVALVIVPAA
                     TAYLLTERLIEMIAYSAMVGALSAVSGYWLAIWIDGSVSGGIVTMTGVSFALALLFSP
                     SQGLVAKARQNRLNRQRFALDMLLMHLSTHERTEEEASESAVGHISAALHWTPEATTR
                     TLRRAIDRDLVIRYDGNLVLTSQGREAVKGLLER"
     gene            complement(11330..12259)
                     /locus_tag="VGR29_09570"
     CDS             complement(11330..12259)
                     /locus_tag="VGR29_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV2073876.1"
                     /translation="MSLLHDLIFDYTLRNVALGAMLLGIVSGVLGSFAVLRRQGLLGD
                     TLSHAALPGICVAFMLTNSRSPIILLIGAGIAGWLGTMLLLRVVRDTKLTEDTTLGVI
                     LSVFFGVGILLLTYIQQQNNANQAGLDRYLFGQAASLVARNVVTFAILGALALAIVVA
                     CYKEFKLLSFDPLFAASIGLPTRGLDILLTSLLVVAVMIGLQTVGVVLMAAMVIGPGA
                     AARQWTNHLHIMLVLAACFGAVAGVSGAVISVQASRLPTGPMIVLALTIIVLISILFA
                     PTHGLVPDRVRRRRQRRNLHANGLPAVAPEAQG"
     gene            complement(12256..13053)
                     /locus_tag="VGR29_09575"
     CDS             complement(12256..13053)
                     /locus_tag="VGR29_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV2073877.1"
                     /translation="MNLTLSRPSPPVIRDETAAVTVHDLTVAYRDAPVLWDVDLTIPT
                     GTLTAVIGPNGAGKSTMLKAILGLVPVAAGTVEIFGHTSAKQRQLVGYVPQRGSVDWN
                     FPTSALDVVTMGTYGQLGWFRRPGKAEHERAMHSLEQVGMADFAHRQISQLSGGQQQR
                     VFLARALVQDAELYFMDEPFVGVDAQTERAIVTLLQTLRSQGKTVICVHHDLESAPEY
                     FDWLVLLNVRKIASGPFREIFTADNLAKTYGGNTNLLKLREHEATAQ"
     gene            complement(13050..14051)
                     /locus_tag="VGR29_09580"
     CDS             complement(13050..14051)
                     /locus_tag="VGR29_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEV2073878.1"
                     /translation="MSLNTRTPISRRNLIASALITVGAVAAGVGFTNNPAASGDGKRP
                     MVTATIGMITDVARNIGGEHVRVNGLMGPGIDPHLYKPSAGDIIQLGNADLILYGGLH
                     LEGRMSETFEKISDSGRKPTVAVSESIPEERLIVVGEGPTWDPHVWFDVSLWRIVAET
                     IAESLIEMFPAHADAFAENAERYIAKLDEVNAWVFEQVERLPESQRVLVTAHDAFSYF
                     GRRYGFEVRGLQGLSTASEAGAGDVQELADFLAEREIRAMFVESSVPPATIEAVQAAS
                     RARGWDVAIGGELYADAMGEEGTADGTYIGMVRHNVTTIVTALLGDGETLAASMEIV"
     gene            14474..15334
                     /locus_tag="VGR29_09585"
     CDS             14474..15334
                     /locus_tag="VGR29_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2073879.1"
                     /translation="MGQRQMATTRFAQNDLARLSYEVAGPAHGPVVVLLHATLGDRIS
                     LRPLRERLEGQAKVILADARGHGASSGLAGRTLSSTDLANDVVAILEAEEVTDAVHLV
                     GHGQGAISALELAHWSPDRIASMVLIEPDALSILEGEADDAVSEAREGARKANREAAD
                     HSYKGLADKALNRYLDRRWGQGWQDRLTKPRLAAVRRSVQALSASLDAEERFRILPEH
                     LEALASPVLVVTGEDSPEAEQHIADRIASWIPGSDRLVVESLPGGAPFSPSDGGAAGP
                     IADWVLARFG"
     gene            complement(15577..16170)
                     /locus_tag="VGR29_09590"
     CDS             complement(15577..16170)
                     /locus_tag="VGR29_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2073880.1"
                     /translation="MTEVSAATPTGARERILTESFRRFSELGFAGVSMQQIADAAGVT
                     KATLYHHFQDKEDLFVEVMQSGFARSEAGLAETVNTGQSLGERLTAFATYLFSSERAD
                     LNRLFGDFHQHVSPERQAAFWKTYQRPWTYLEGPIKDAIASGEIAPGNPNLIARVCFT
                     AFVGQMQIARFEPDAPPPDAALARQIVDMLLNGLRPQ"
     gene            16341..17969
                     /locus_tag="VGR29_09595"
     CDS             16341..17969
                     /locus_tag="VGR29_09595"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEV2073881.1"
                     /translation="MKQQPATGMPASAASGPDHGPQYLSHSQIVGVMIGIMSGMFLAA
                     LDQSIVGVALPRITSDLGGLEHLAWVVTAYLLTSTASTPLWGKISDLYGRKRIFQTAI
                     VIFLVGSVLCGLAQNMPQLIIFRAIQGIGGGGLMSIALAIIGDVVPPRERGRYQGYFG
                     AVFGVSSVAGPLAGGWLTDAINWRWIFYINVPIGIAALVITSISLKMPVMRRDHRIDY
                     LGAGLVVSAVSLLLLYVNWAGDHYGWGDPRALALLIASIVLTLAFIVAENRAVEPILP
                     MRLFGNQIFSVGNAFGFLSGVAMFGGVIFLPLYFQGVMGMSPTRSGLAMVPMVAGILT
                     MSIVSGLLVTKTGRYKIFPILGAFLIIVGLLLLSQLSVDTPYWQIAVYAFVFGLGLGF
                     TLQTITVAVQNAVPFRDMGVATSSLTFTRSLGSAIGTAIFGAILNSRLATHLVDAMGE
                     AGVQPGQLSSEATGDIQAIQNLQEPVRGLVLGAYTDALTDLFLYAVPFVIVALVVALF
                     LKEIPLQSGAAPGPGEPTGNPRSAEETIPVMGGH"
     gene            complement(18471..19415)
                     /locus_tag="VGR29_09600"
     CDS             complement(18471..19415)
                     /locus_tag="VGR29_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEV2073882.1"
                     /translation="MSFNDKPFRFGVQITRTRTGDEWRETARRAEALGYDVFLMADHF
                     GNQFSIGPALASVAEVTSTIRIGTLVWQNDLRHPALLGMEAATLDVLSGGRMEFGIGA
                     GGSFPPEYPWTGIPLDPPGTRVSRLAEALPVFKRIWSNEPFSFEGQHYTFQEYTGHPK
                     PVQQPHPPIMMGAGGNRMLKLSAEHGDIIGLLPSMLAEGGFADNEISMEAFAAKVETI
                     RAHAGERFPEIELNILVQMFGVTDDRDAEIERRRVQMELKDEDMGWFDSPMVYIGSVE
                     EIVARMQEVRERVGVSYFVVFEPMMEEFAPIVKALAGK"
     gene            20033..22690
                     /gene="alaS"
                     /locus_tag="VGR29_09605"
     CDS             20033..22690
                     /gene="alaS"
                     /locus_tag="VGR29_09605"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV2073883.1"
                     /translation="MTPVNDLGIPWQSGIDIRRKFVKFFESKQHLHVPSSSLVPPAED
                     QSVLLTTAGMQQMTPYFLGLEQPPAVRMCSVQKCFRTVDIDEVGDESHSTFFFMLGNF
                     SVRDYFKEESLAWSWEFLTEWMKIPADKLYPTVHPEDEEAYLIWRDQIGVPEERISKL
                     EDNWWSAGPVGPNGPDSEIYVDRGEQWGCGQPDCGPGCDCPRYLETWNNVFMQYNTTA
                     DGVRTPLSKPCVDTGMGLERLTLLVQGARTMYETDFYQPIIAKASALAGVTYDADPQI
                     DKSLRIVADHVRGSVFLIADGVLPGNEGRAYVLRRVLRKAIRHGRLLGIEQAFMADLA
                     EVVIEQFREAYPELTDRRSQILKVIAYEEASFGKTLASGMNRLQVLTGELRQRGQRVI
                     PGDEVFRLYDTYGFPYDLTAELASEEELTIDEEGFRLAMERQRQTSRSGAAFRREGRE
                     RADLYASIQPRTEFLGYEATRADARIVAILGPDGNLEQAEAGEQVEIVLDRTPFYGES
                     GGQVGDTGEIRTETGLVNIDDTRRPAPDLFVHRGIVEEGFIRVGESATAQVDSERRLH
                     IRRNHTATHLLHKALRLVIGDEAHQAGSLVAPDRLRFDFTALEAVEPEQVRSIVEIVN
                     NEVTQDIPVQTTVMDQKEALAGGATALFGEKYGEKVRVVEIGDFSKELCGGTHVDRTG
                     EIGPFVITSEGSVASGIRRIEALTGNAAVERMLGQQRLIEELGRDLKVSWSEVPLSVK
                     ALQERTRAQEREIEKLRGQLAGAKSGDLLQNAVEVNGVKILSSRVDVTDKGGLRQLGD
                     RLRDSLQSGVIVLGAVVDERPSLLAMVTPDVVSQGVRAGDIIRQIAPHVDGRGGGRPE
                     LAEAGGKDPEGIDAALAAVAGIVTGQTGA"
     gene            22690..24417
                     /locus_tag="VGR29_09610"
     CDS             22690..24417
                     /locus_tag="VGR29_09610"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEV2073884.1"
                     /translation="MSTDRQQPPVRITIDPNDSLPVILQQLREHGGKSVVIDIPDHCP
                     VLLTATEFRTMKELAERERISLSLRTDDRLRHQLSSMFGIKNVGDAGAQSPNDGWRPP
                     PTMLGSPRAFGTWNKNDDEDDGIVELEPGTRSRRRRRGEVIETNTYTKRSDDDEGISS
                     LEYLNEPKELLTAKNIGRIVGVLLVVALLLAAAGWYYMPAVTVDATLRRQDVSNQLLY
                     SVAAPGAQLPSDIAFTADATQGSATVNFTITIPTTGVERTPEGTASGSALLRNPGEAP
                     VTVPAGTELSVYAGPTFTTNEAVEVPPAANGVAGEASVGVTATAPGSAGNVEAGLLTG
                     RLADLGVFYSNRETPIEGGTDIEVMVVAQEDIDRLREEVATNLDRAAAEGWIAQLPEG
                     QSVVVPSVDTGEPTYEITAQVGEKLDEITATGTVDATGLVFDAATVEQQTLEFFRTAL
                     QEEVPEGYALDPESVTLSEPAVIAEAPDNVQYQVEASGLAHAVYDESVLEDLSEDLAG
                     ESWDGAESTLAGVEAFESWEMSTSPGWWARRMPQTGDRITINVEQAPAASDAGEPAPS
                     PSPGEGDGS"
     gene            24420..24854
                     /gene="ruvX"
                     /locus_tag="VGR29_09615"
     CDS             24420..24854
                     /gene="ruvX"
                     /locus_tag="VGR29_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV2073885.1"
                     /translation="MGERLLGLDIGGRRIGVAVSDEMGMIASPVAMIARQSDVAGQLK
                     ELVARYNAAKLIAGLPVGLSGREGPQAAEVRAYTDALALEVGVPVEYWDERLSTSIAE
                     QSLIAQGTRRNKRKERVDAVAAAVILQSYLENQRWKLSTGRR"
     gene            25139..25426
                     /locus_tag="VGR29_09620"
     CDS             25139..25426
                     /locus_tag="VGR29_09620"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEV2073886.1"
                     /translation="MKKLIIAVVQNEDADAVVDALLEEEFRATRLASTGGFLRRGNTT
                     LMIGADEEQVDAVLDLIRRHAHATGDAQSAGQSQPAAATVFVLDLEEYQRL"
     gene            complement(25685..27019)
                     /gene="aroA"
                     /locus_tag="VGR29_09625"
     CDS             complement(25685..27019)
                     /gene="aroA"
                     /locus_tag="VGR29_09625"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV2073887.1"
                     /translation="MSMFYSHSSFRANVLELAPVSRPVVGTANIPGSKSITNRALLIA
                     ALANGESILTGALFSDDTRYMAAALNQLGIRITGDQQAESFTVVGCGGKIPVHEADLF
                     VGNSGTSMRFLTAAVALGHGTYRLDGVPRMRERPIAPLLDALDQLGVNAASEEGTGCP
                     PVRIVAEGLPGGAVTLPGDKSSQYFTGLLMAAPYAQQGMTIKVDGDLVSKPYIEITVD
                     VMAAFGAEAEIDDETWQTFRVEPGQAYGGRLFHIEPDASNASYFFAAAAITGGEVRVE
                     GLGTDSLQGDLDFVDVLEAMGAEVEMRESETIVRGPTDGALKGGDFDMTEISDTAQTL
                     AAIAPFADGPVTIRGVAHNRIKETDRISAVATELRKLGQEVEEFEDGMTITPRPVTPA
                     DIETYDDHRMAMSFGVTSLRAHGIRILDPECTAKTFPDYFERLISLTGAERM"
     gene            27224..28495
                     /locus_tag="VGR29_09630"
     CDS             27224..28495
                     /locus_tag="VGR29_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073888.1"
                     /translation="MSGLFGSTILEVVLGLAFIYTLLSLLCSSINELLAGFLKLRARN
                     LERAIQNILCDEDLVREVLGHPLIKAMGSTRAETTGVKVGAGQGTRKDFAGKPSYIPS
                     RTFAVALFDTITPPTGRSITVGDVQRAAEEMAGAVEKRAADLTPRETIGRALSSLINE
                     SRDPQQLFLTVDQVKEVVNSLADPSLDDETRQKLSAAATLDDLRRLIERFPDDTIRGR
                     LIDMLAESQTGLDRARLSIERWFDDAMDRASGVYKRRTQWWLLMIAAAVTLCIGADMF
                     RLYDSLATNPGLRTALVAQAEQQAAAPASDGVLPSETNDGEVSDTPSTDSAEGSLPAP
                     SVGEIFDELDQTSLPFGYSDRPGMDGEEADARDWFRWLLTKISGLLVTMFAIALGAPF
                     WYDTLNRVSNIRAAGKPPASTDASAAPSRST"
     gene            complement(28632..29597)
                     /locus_tag="VGR29_09635"
     CDS             complement(28632..29597)
                     /locus_tag="VGR29_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEV2073889.1"
                     /translation="MSQSRILVGMPLLRRASVAIILALLFGLLALSSPTSAADGDPIK
                     VVASSSILADWVSNVTGDSADVTSIVPAGGDAHTFDPDPATVASIADADIIFAIGSGF
                     EPWLADMIEASGTEAAYVEVTRDMTLLGADHEDEENHDEDEGDPHIWGDVSNAISSVE
                     LIGTALAEVDPDNADTYRANAEAYIEQLKELDGAIREQVATIPEANRKLVTSHDTFAY
                     YAKAYGFDIIGTALGSSSSESGDPSAKEITTLVEDIQEADVPAIFAENVVNPSLMQSI
                     ADEAGVELAPTLYTDALGEEGSDGSSYIGMMTYNTNTIVSALGDS"
     gene            29873..30997
                     /locus_tag="VGR29_09640"
     CDS             29873..30997
                     /locus_tag="VGR29_09640"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEV2073890.1"
                     /translation="MLTSLRKAVLATLALWLIVGGTFGASPASARQSEDEEEEVVRTV
                     IDLLRLEGQGDLDRLYDAMAPESRGIIPRQAFVNWYSDEDRTVPADEPEIESITFDDW
                     ESDATGDEYEDVAVVEYSVEVEAGRDEDIRSAEMILWNDGQVWRWFFADMEVDAEDVA
                     EEDEWTVDYETPYQTEMYQNLDMFWAQIFDNAGLAYHPPVDMVGVLVEPTRTGCGVEN
                     DITQMAVYYCTLDETIYYDPAFRDAMVENIGDYAWEHVIAHEWGHHVQNLLGLTTAMD
                     PELEGGQYTIEHELQADCLAGMFGQDARARGLIRSRDLNEAIDITGIAGDAAGTSWDD
                     PSAHGSAEQREESFWIGYNDGFRGCHIRLDVEGGDGNNRW"
     gene            31142..32455
                     /locus_tag="VGR29_09645"
     CDS             31142..32455
                     /locus_tag="VGR29_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEV2073891.1"
                     /translation="MTMAHTQTENPLDVFTDVTLPRWALVEQRLDDTSVGDFGEAIAR
                     EFARPEIRSTIGPGQKVALTAGSRGIDRIAEVLRAVVDQVRGLGAEPFIITAMGSHGG
                     ATAEGQREVIAHYGVTEETMGAPIRASMETVHLGTIEGNVPVWFDRIAYEEADVVIPI
                     GRVKPHTSFRGPTESGLMKMLAIGLGKQHGASWFHSHGIGTFGDLIPRVAQHTLSQVN
                     IPFGIALVENGLSKMALIEAVPREGMYERECELLEIARGMLAVLPPVAKVDVLIVDEI
                     GKDISGDGADPNVVHRGVANEIDFSEDRPSIQRAVVRGLTEDTEGNASGIGMFDFALR
                     RAVEAMDPIPTYMNMLTAKAPQGAKIPITVENDRQALSLAIASALRVEEGTAKILRIR
                     STKHLTQMLLSEPLLAELEGMDGIVRIGEPMDIGFDAEGMFNAEL"
     gene            complement(32850..33506)
                     /gene="cofC"
                     /locus_tag="VGR29_09650"
     CDS             complement(32850..33506)
                     /gene="cofC"
                     /locus_tag="VGR29_09650"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEV2073892.1"
                     /translation="MRTVAVVPIRGAHGAKTRLSPIFAEHERASLAWSMLTHVLDVLD
                     ACPGIDTTLIVTRESDETLRQIGPDPRHTVLFQPVEHTGLNAALDLGRDWALTHSYDA
                     MLVLPADLPILAPADVESMVRPDAPAVIAPDRHGSGTNALFLRLDSAHPQQFAFAFGS
                     QSYHRHHAEADRLGIQMKTARAPGLECDLDSPEDWRDLPIAIRQRLLDCVQRAESMAR
                     "
     gene            complement(33506..34453)
                     /gene="cofD"
                     /locus_tag="VGR29_09655"
     CDS             complement(33506..34453)
                     /gene="cofD"
                     /locus_tag="VGR29_09655"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEV2073893.1"
                     /translation="MPEYGNVVALAGGVGGAKLVQGLAAVLPKDQLSAIINTTDDFRL
                     YGLYISPDVDTVMYTLAGLANPTTGWGIAGDTRATLDAIARYGEDPWFTLGDQDFATH
                     ILRSHYLQEGATHTTTTARLAAALGIEATLLPMSDDPVATEILTADGWLGFQDYFVAR
                     RHDDEVLDVRFKGIEAATPTSEVLEAIASATLIIFCPSNPVVSIGPILALPGIREALI
                     DAEAPIVAVSPIIGGRALKGPADRMLASRGVDVSPLGVAEIYRGIIDGIVIDHADAAL
                     ETPLAEQGLQVHVGQTIMGDTSDRGRLAQEILDVEWTRA"
     gene            34738..35736
                     /locus_tag="VGR29_09660"
     CDS             34738..35736
                     /locus_tag="VGR29_09660"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2073894.1"
                     /translation="MFQDLHIIDVHAHFPIRGDASQGGSRTRETGRIDNPAAVERQEL
                     RTQQLERMRHQWRMAFDFPEPETEPRSSEEQADRWVAELEKYGIERIGFVTGRDNDEL
                     ARVVKMYPDRFIGFAHHDITRPDAGEELERAVGELGLKALKLMAPRIPILINDASLYP
                     VWETCERLGIPVLCHFGMLGAAGGISYHPNISPAVLEPVARDFPTVDFIIPHFGIQHV
                     TDLLFLCWSCPNVNVDSSGSNQWVRWMPYKLTLDDLFQRFYETVGPERILFGSDSSWF
                     PRGFALRYLQDQLRICRFMNMPHEHLQLIFGGNAARILNVPLGDIDARKAAEGQAG"
     gene            37168..38550
                     /locus_tag="VGR29_09665"
     CDS             37168..38550
                     /locus_tag="VGR29_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2073895.1"
                     /translation="MGSSEFDPKSVLRRAADRRSVLKYGAASAGAFGASRWLGASAQD
                     ASPAASPVDGPADFGTGSLELSGDVTIQYWQYELAAKTQLVEELIPEFEEANPGITVE
                     HVNFPYDDFRQQVAAAVQAGEGPDVLNVYYGWIPAYVQQQFLVPLPQDIFPAEVIRDA
                     YFPTVQVAEVEGEYYALPTAVRTLALFYNKDILEAAGVEPPSNWEELVEVAKATVQKE
                     GDNFEVVGFTWDIGGQGHNWWREALNRQNGQIPYTDDRRELLWTEPECIEAFEYAVSF
                     LTEHGVTQAGFQTDGATAFAAGKAALHIDGSYRLGSLAIDAPDLNYGVVPLPEHKEKA
                     SFASFWANGITRNAAEGDKLIASAKWIDFLSSAEVQRRWTPAIGELPARQALASDPAL
                     LENEKLVAFIESLPYSYATFFVNESTLRQAVIDAFDQVVLNGVDAETAVAEAEAQVQE
                     ELDEYFAQLD"
     gene            38621..39511
                     /locus_tag="VGR29_09670"
     CDS             38621..39511
                     /locus_tag="VGR29_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2073896.1"
                     /translation="MSLSLQRRRWLWGYAFIAIPLFFFLFVRIGPTIYAFWMSLHEWD
                     PIALDRPYIGLENFRTMADDETFWKSLRNTWIYVGVGVPAGLIISLLIALGLQRMIRY
                     TGFFRVLYFLPYITSLVAVGWVWRWMYMPNGLFNDILGWVGLGPYKWLGSPDMAIYSI
                     IATTVWHGLGFQVVIFLAGLESIPEDYLAAASVDGASGWQKFRDIVVPQINPTLVFLI
                     VTGVISNLQVFTQIRAMSSGGTGGPLDSTISMVLYVYQEAFQSLPSHMGYASAMTVVL
                     FLMILAITIFQLKVLQRDTE"
     gene            39508..40437
                     /locus_tag="VGR29_09675"
     CDS             39508..40437
                     /locus_tag="VGR29_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2073897.1"
                     /translation="MTTKTYFPDEPGVPLAGVTNDDAAASRRTAMARVRRDQRRRRVA
                     RIALLLILLLGGLAMIFPFVWMVVSSLKKAQDIYTLSLIPPDPTLGNYTEVLQRTSYI
                     RWFLNSIIVAGITTLSVAFFDSLAGYTLAKFRFPGSTAIFLLILSTLMVPTEMLVIPW
                     YMMSIEFNWTDSYWGIMFPGVISAFGVFLMRQFFMGLPNELIDAARLDGFSEFRIFWK
                     IGLPLVKPAVAALCIFTFLGNWNAYIWPLIVIRSDTMRTLPVGIAFFSSEAQSSFHLI
                     MAAASLATVPVIIIFLIFQRHIIKGIALAGLKG"
     gene            40541..42034
                     /gene="argH"
                     /locus_tag="VGR29_09680"
     CDS             40541..42034
                     /gene="argH"
                     /locus_tag="VGR29_09680"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV2073898.1"
                     /translation="MSNDTAGTFPHPAYAQNVLEPAFEESKEHLFAHMIAANEAHAIM
                     LAECGVLDPAQASALLTALGAVKQQGAEAFRYSPEIEDLFFAVEGRLIELVGPDAGGN
                     LQIARSRNDLAAAMSRMMLRERLVGARQQVLHLRSVLLDMIEQHIETLMPGITHTQPA
                     QPTTLAHYLLGVLGPLERDSDRLSCAWRRTNRSSLGVAAFTTTSFSIGRELTADLLGF
                     DGYVENGYDAVGANDHLLESVQALVNCVGSLSRFVYDLLVWARKDVDIIRIDDAFIQM
                     SSIMPQKRNPVVLEHVRVRIGWVYGEASAVETIIHSAAFGDTNDVEDPMFVPLNRAFD
                     AAEMVLRLLAAVLSTAAFNVSGMEQRAAEGNTTTTALADALVQEHGIPFRGAHTIVSR
                     IVSRTMSDDVPITAELVNEVSSEVLGRSLDVTQEFVANALDPWAFVNARTIPGGSAPE
                     TTRQALESARLHLDEDRGVLEADNAAVNHASAERQRRIDALMAGASS"
     gene            complement(42187..>42797)
                     /locus_tag="VGR29_09685"
     CDS             complement(42187..>42797)
                     /locus_tag="VGR29_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2073899.1"
                     /translation="FRTLEHLKRTKRTGWIDRGVPREQVESVADHTMMTALIAWFLAH
                     EDGTLDADRVLKLAIIHDMAEALVGDRPPYEADEIPPPGDLDALRAFFSVRHLRSPEN
                     RAAKRQDEERAAAHLIALMPESIGEEMRSLWSEYEEQSSPEARFVKEVDRLEAFLQSR
                     TYLRGHPDIPVQGFTDMAQKEIDHPLLTAIRDAALNVEDTPG"
BASE COUNT         7790 a        13062 c        14079 g         7866 t
ORIGIN      
        1 tcctaagcgt gttccgacgc ccgggacgac gaagcgcggc ggcgaccagg gcagacaagc
       61 cagcaaacgt gtcgacatca ctgcccgcca cagcctctgc tgctggcagg gtgaacccgc
      121 caatgaagac cccgaggcgt cgaaagagca cctgctgctc ctggcaggag gtcgtgactc
      181 caactgatgg cgtcccgcat ggcgtgaagt cgtggcggtg cgtcgcgtgg accaccggtc
      241 agcagatcga ggcggtgctc aagccgggcg aggagtgccc ggggtggcag catggggact
      301 cttgacgcgg ccaactcgat agccagcggc aatccatcca gctgggcaca aatggcatcg
      361 atgacatcag ccacctcgtc ggtggccgtg aaacctggca ccagcgcccg tactcgctca
      421 atgaaaagcg ctctcgcctc ttcgccgggt aatggggcaa ggggcagcag gtgctccccc
      481 gatacgttca ggttcgttct gcaggtgcac aagatggtaa gccgagggca cgctgcaagc
      541 agttcggcca cagccggcac tgcatcaaga aggtgctcga caacgtcgag cacgagcaga
      601 agttgctggt tcgacaggag tgctcgcggt cgcgatagca acggcgtgtc gggggtgtcc
      661 gggacgttga ggtaatgtgc cacggtgggc agcagcatga ccgggtcacg gacagcggcg
      721 aggggtatga agaccacgcg gtcgaaggtg tcgtggctat cttcggcggc acgcagcgcg
      781 aggcgcgtct tgccgacccc gccggtgccg gtcaccgtga cgaggcgaac atcgggtcgg
      841 cgtaagagat ccaccacggc ggtgagctcc cggtcccggc cgacaaacgc ggtcaacagc
      901 ttcggaaccg caccgatgtc gggccgctcg ttgagaacga gcggggcgga attcgtggag
      961 ggactggagg gcgagttgtt tgacatcgtc cgctcccctg atatcgatgt aacgagatcg
     1021 cttcatcgct atcatacgct tcgatattgc ggagggcaat cgcctcaacg tctgtcgccg
     1081 ccactgtggt agcaccatca ccggcgcatt caatgcggcc gctcactgtt gttgaccgcg
     1141 tatataagcc tgctcgcttc tcaatacccc tcgggattgg ggcgaggtga ggggcttcag
     1201 gcaactgagc gacgaagggg atggtcacgc agcccttggc gtcagggacg tcgtctctgc
     1261 cgcagcgggc gtgcccggag ggcgcgctgc cgtgcgccaa cgctgtgcag accggtggta
     1321 aactcgccac gacgcagatg gccgaacacc gcccggttcc catgccagcc gttgaccgag
     1381 agaagggcga cgatggacac ggcagtattg accagggcgg tgatggcgat ctcggagagg
     1441 gaataggcgg tgatgacttc gggagccgcg taggcactga gagcgacaat atctatggcc
     1501 tgggtcgtgc ccaggccccc gggggtgaag cggactgtcc cggcaagagc gttcgaacca
     1561 agcgcaagcg cgaccgtcca gatggtgaca gggatcccga acgcggagag catcacgaca
     1621 taggcgccgc aacggaacag atatgaggcc agcgacggga ggaacattag ccggacatac
     1681 cgtgccggac ttccgaggat cgccagcccc tcccgaacct gctgtcgcac ctgatccagc
     1741 tttcggcgtc catagatgac tgccacaatc aggaggatca cgatgaagcc cgcaacaacc
     1801 cctgtcaaca caggctgtgc cgacgcaaag ctcgataccc gttcgatcgg gccttggcgc
     1861 tcctggggtt gctggggaag cccgacagca accagggtgt agctcacgga cgcgaacagg
     1921 ataaaggcga gattctgcac gccccagatg gtaaggagtt tcggggcacg tgcaccggga
     1981 attgccaggc cgatgattcc caacattgcc catgtcccgg cccggctcgg cactacggcg
     2041 ttgacgatgt cctggccctg gtcaatcccg accacgaagc ggtaggacag attggcgtgg
     2101 ggccatgtcc agttgagtgc gttccgccag ctcagcgatg acaaggcagc ctgcatcact
     2161 ttgaaggtga cgatcaacac cagatacgcg ggtgggatcg agagcatctc gcgccaggtg
     2221 gcaaccgcca atgcacgtag gtccgcatgc aagacgatga gcaagatgac agcaaggagc
     2281 aacaggaggg cgagccacct cacccagcgt cgccggccgg tggactcggt tgaaagggtt
     2341 gcagtgttag cggtcatcat gcaggccgac gttggcccac tcctcgttca tgttattggt
     2401 cgggtcaccg ggcggcggtg tgggtgtctg tgatccctcg tcgacgcagg gggagtgcat
     2461 tcggtgagtc ttacaccatt cggaccgcct tcttgcggtc agctgatgac gcaattccca
     2521 tgcacacccg gggcacgact cggcactccg aaggagcatg agcaactgag ccttgaggat
     2581 gtcgccaaac tggcagtcgc atgcggggaa tctggcgatc gcagggaggc gctggcgtcc
     2641 agtgtccgcg gcaccaaagc gcagatatcc ctggtccgtc cacggtttct tttggcaagg
     2701 aacgtcgact gccttccacg acgcgtatct cccgaggaga tgaaccggcc ctcctgggct
     2761 catgtggagg tatcagcctg caggcgttct gcgctccccg tgtactggtc aagagtggag
     2821 acgaccttcc cgttggcatc gtgcgcggtg atgctcccgg tggccgtcaa ggcattgccc
     2881 gccctgtcca cctcaatcat cacccgcatg gtaaccaggt caacaacgcg gtccgacctg
     2941 aacgcgggtg cctccggaac agcgtccgac gcgaagactc cccacgcgcg tgcgacctgc
     3001 tggaaggtca ccacgacgtc ggcggtctgc tccccggtgg cccgccagag gccaatccca
     3061 accccgagga gcggatcgta ctcaacatag gcgccatcgt cggcgatgac agcggacgag
     3121 gggtgaacgg ggttgctcgc ccactgccat gttccaacca ctgggtggcc gtgccatgga
     3181 gcgcgttcgg cgacgggaga tgcctcctgg gcagcatgag atggcgtcgc taccccggtc
     3241 aggagaatcg ctcccgtggc gagaccggtt acggcgctgc gacgcgacaa cggcgtggta
     3301 gatcggtgga tcgtcatggc tgacaccgtg gaaacgcgat gcacgatagg gccgggtgga
     3361 ttcctggata atggtgtcac gatagcacgc aacgatgcct ctgtcacgtc catccgtggc
     3421 cgcaccgtgg accagcccta cagcgcacgt ttcgccttgg acgccgatcg cgtcgcgctg
     3481 ctcttcacgc gctatcgggc gttgacgagg aggaatggtg gtcccgggca tgccatcgta
     3541 gggaacgtac ctggccgcgc ataccctgat cgcagacaca tcctggtcgg cgcggccaat
     3601 gtcgacccgc atctgtaaac tcgttcccac aagggaagca tcgtgccggt gatccatcgc
     3661 ttcgctccgg atgaggatcg gtggtagagg tgagctcgga gggccaccag ctaccttcat
     3721 catgaccgaa gggaaggagc ccccggcgaa gctgctgcct gcagggcaga ttgtgcggcc
     3781 gagcacctcc tgaaaggaat ggcgtcggtt tttcggcaac atatatcgag aacagctggt
     3841 ctcacaccgt cgtctggcca gtagcatgcc ttgggccaag tgatacgcgt gtgccggcaa
     3901 gatcgctcga tacactctct tcgctcagat ctgtctcgaa cggagaagaa tcatgaccca
     3961 ggaaaatcac ctccagcagc acattgagac catcgccagc tggatgatcg aggccaggca
     4021 cattgtcgcc ttcacgggcg ctggtatcag caccgactcc ggcatccccg actatcgcgg
     4081 tcccggcggg gtgtggacga gacgcgatgc cggcttgcct ccaccccagt ggcgggtcgc
     4141 gccgaatcag gtgcagccga acgcgtccca cctggccttc gtcgatcttc agcgacttgg
     4201 caagctccag ttccttatca cgcagaacac cgataacctt catcgccagt ctggcatccg
     4261 ccctgcgctc ctggcggagc tgcacggcaa cggacagctc atgcggtgcc tggactgcga
     4321 ccggctgcac cctcggcagg acgtagggtg ggatacgcgc cggtgggggc cgggctaccg
     4381 tacccagaac ccagtgacgg ggcagccggc ctgccccgtc tgcggtggcc ggcttatctc
     4441 gtcggtcgtg aactttggtg atcccctgcc gcggcacgag ttgaccctcg ccgaccacca
     4501 tgcccgctct tgcgatcttc tgctcgtcct cggctcctcg ctcgtcgtcg agccagcagc
     4561 ttccctggtt gggcgggcga tcaggtcccg agcccgcgtg gtcctcgtga atcgaggtga
     4621 aaccccctat gatgaggccg tgaagctgcg cctgtggagc ggcatcagtg aggtggtccc
     4681 accggcggtg gagcgggtga agggcacgct cggggagcag cctgagagat cgtgagtgga
     4741 cgatgtcgct ggtccaatgc cgtcaacata aggggctggc ccatagagcg agggcgaccg
     4801 gccagataag gacgactggc atccgcttcc cgggacaccc ggattggggt aaacatacgc
     4861 aagagcacgc tctcctggtc ctccgcgttt caatctcatg aatgtgggaa ggtgcaccat
     4921 gccaggcaca actcgaccgc tccttgcgat tacgatgggc gacccggctg gaatcgggcc
     4981 ggaggtggtc ctcaaggcgc ttgcccatcc cgaagtgttt gcgcgggcgc ggccgctggt
     5041 cgttggcgac cgttccacgc tcgaaagggc agcccactgg gtcgaggcaa cggacctccg
     5101 gtatgacgtg gtcaccaggg ttgaggacgg ccagtacgga ccaggggtcg tcacactgat
     5161 ggatctgcaa acatcacctt ctggcggata tgcagtcggt caggtaagcg cagatgccgg
     5221 tcgagccgcg gtggactttg tcttccgggc atgtgatttg gcaatggagg gcaaggttga
     5281 tgcggttgtg accgcgccct tgaataaggc cgccatgcac gcggcgggct tcacctatcc
     5341 gggccacacg gagcttctcg cggagcgaac cggggcggaa cgggtcagta tgctcctcgt
     5401 tggaccgtcg ctccgtgtcg ttcatgtgag tacgcatgtg gcgttgaccg aggcaatcag
     5461 gcgcgtcacc cggcagcgcg tcgaagatgt catcgacatc gcccgggatt cctgccgggc
     5521 gctcggtatc gagcaccctc gcatcgccgt ggcaggcctc aaccctcacg caagtgaggg
     5581 cggccttttt ggcgacgagg aatcgcgtga agtcataccc gccatcgagg cagcacgggc
     5641 gcgaggtttg aatgtcagtg accctcaacc tccggacacg gtcttcctgc gcgcaaccaa
     5701 aggggagtgg gacatcgtcg tcgcgatgta tcacgaccag ggacatattc ccatgaaact
     5761 gctggcattc gattcgggtg tgaacgtcag catcgggttg ccgatcctgc gcacgtcagt
     5821 agatcacggc acagcgttcg acatcgccgg cctcgggcag gccagcgaga acagcatgtt
     5881 ggcggcgatc gatgtcgcgg tccaaatggc cggttcgcgg tcagcgtctg gtcttgccac
     5941 gtgaccaagc gacaacgagg ctagcatgac acagctggcg gtgcttgctg acgatcttac
     6001 cggcggcgct gacgcgggcg cagcctttgc cagtgtgggg ctggcgacgt cgattgcctt
     6061 cgcgagcgac gctgatgact gcgacgttct cgtcgtctgc acgaacagtc gtgggattga
     6121 taggaccacg gcggcggacc tgaaccgacg tgcggcaagc gcatttatca cccggccggt
     6181 gtcgcgccgc ccgcgatggg tgtacaagaa gttggactcc gcgctccgtg ggcatcctcg
     6241 cgacgagtta ctggccgtga tggacggtct gggcgagtcc agggcgcttg tcgcacctgc
     6301 cctcccggct gaggcacgca ccacggtgga tggcaggcag ttcctggacg gaagtcggct
     6361 cgaggcaacc tccctcggtt cagcccggaa ttcttccgac ctggcctccc tcttcgggag
     6421 cggaggcgat gtgccggtgc acgctctgga tctcaagacg atccgcggcg ggcaagggga
     6481 aatcgagcgc gtcctggggc agatggggcc ggggttggtg attgctgatg ccgagacgga
     6541 cgccgaccta atgctgattg cccgggcctc cctgggcagc gatcttcgtc tgctggccgg
     6601 ttcggccggt cttgcagggc aactcgcgcg ggtactggcg ccggctcctg tctcgcacaa
     6661 cgagataacg catcgtcctg cgaatccgat cctggtcgtc gccggaagcc tgcatcaggc
     6721 caccgccgcg caggtcgaga tcctgcaggg agccggcctg ccgatcgtgc gtccggtgga
     6781 cgtcctgctc gatgaatcag tgcgagcgat agaacggacg gtggatgagg tggcgatgca
     6841 cctcttagct ggtcgatctg tggtcgtggc aacggcaggg cttccgtctt cgcagcccgg
     6901 ctcaacatct gttgtctcgc gcctggcgga tattgtcgcc gcaccaacgg tttgccgtac
     6961 cataggtgga cttgtattga cgggaggcga gacggccgcc ggcatacttg cccgacttga
     7021 agcaatcgag ctgcgcctca tgggagagat ccgtcctgcc atgccgtggg ggatactgcg
     7081 ctcgtcccac atacgggcgg tacccatcgc gaccaaggcg gggagtttcg gcgacgacga
     7141 cgccctgctc gcatgcgttg aacacctgac cgggacgaga ctccggtaga ctggtctgcc
     7201 gataaaggga tcagccgggg agaagcggtc tcgcccggga cggggagcgt tcgatgcctc
     7261 gccagtggca gcatcccccc acctggcatc tacctgctct cctcacgtca gatcatcccg
     7321 ggcatcctcc tcgcgggtgt caaaggtggt cggcctcaac cgaatcccat gctgtgctga
     7381 cagtgtctct cgtgagagcc tcggagtctc tgcccccacg cacgacgatg aggtccatgg
     7441 cctctcggta gttttggggc aggtctaagt gaccgcctat acggttgatg gtcaaggtgg
     7501 atcatcgatg acgggactcg ggtacttttg gtaggggcga agaccttcaa tcgacggtcc
     7561 ggcaagccat aacaccctct ggtatacggg aagattttgg caacctggtt gaccgatctg
     7621 gtcaccgccc ggctcaaacg gttgaacgcg tccgacatag gtccgtcaca gagtggggga
     7681 gtcccgggga agcggatcag catgtatggg ttagtggaac gacgtagaag cggaggcgaa
     7741 tggcatgcaa gcagtcggta tcgtcatcct gctgaccgtg ttcgcccttg cggtgatccc
     7801 gctgaccatc gcgtactacc tgtggctggc aagaaccgtc ctcccgaggg tagcggctgc
     7861 cctgccacca cggagcgcac gtatggtcct cgcgatgtgc atcgcattgc cctggttcgt
     7921 agtcgccctc gccgtctggt ggttccgggg atgactctcg gcggtggtat gtgggcttgg
     7981 cctggccgtc catgaccatg ctctacccag tgtcatcctt cactaattga ccaaaagtgt
     8041 agcgtcggta tgttggcacc tatattgccg gaaagacatc agaccgtggc acatcgctct
     8101 tcgcggggag actcctggca tgcttttcta ttgcgagttc acctggtttc ctggcaccac
     8161 ccgcatgcag gcggcacgac gcgtcgtcca gcagcatgat gccggggcca accatcccga
     8221 acggatcagg ggctggtaca acctggcagg tggcggtgct ggtttcctca tcgtcgagta
     8281 tgacaatatc aaggagttga ccgccttcct ccagccctac atggacctga tgagcttcga
     8341 tgtgcgagcc atcggcgaac tcaactatga ggagcggatt gagcagttgc gaggcttggg
     8401 cgggcagccg gacacggaag aaccacccgc gaggatggat tacgcctagc ggaccactga
     8461 agctattttt attccctggc ccgcgtcagc cgctcatcaa gcggtatcag gcgctgacgg
     8521 aggagtaatg ccggtcgcag gtatgccatt gtaggggacg tacttggctg agcccgcagg
     8581 gcgaaaagca gagcgagccc ttggccgcgc cgaccgggat gtgcctgcga tccgggtatg
     8641 cgcggccaat gtcgtcccgt atgcgtagac ggtacgcgac gccattggat gcactactgc
     8701 ccgagggcat gatgcgtatc tacgcgatca ctgcccaaat cacgagccca ttattattgc
     8761 taatgtcttt ccgacagcga cgatgtttca gcaggcggtc atagcgaatc acgtactaag
     8821 atgctcaata tcgatgtctt tctcgcggac gttctgtcta ttaacaccca atcctcaagg
     8881 tgcctaccgg gcagccggcc atgggcgcgg atcggccgcg ccctggggga ctcggcaggc
     8941 cggcagtgcc tggcatgccg acagggtggg gcttggtgtt ccgggtcggc tggcctaatg
     9001 gctactgccc aatgccgagg cgccgctcac cgtggatgta aaggggcgct cattgttccg
     9061 ctgattattc cagcgcgtgc cagctggccc cgcccgtctt gagacacaga taaagtgcgg
     9121 agtctagaat tgttgcctgc agtgaccaga agcgcgatta gcgggtgccg tctcggaccg
     9181 ccttacgcac ggtctcatgg gagacaccta ggtcccgtgc tatgtcccgc aaactgtcat
     9241 tcttggctcg tgccgccacc tcaccccatc ggttcgctgg aatcaggcta gccctcgctc
     9301 ctaccgcgaa gcgtctagcc ccacggcgct gagctaattt caaatggtgt tctgggtata
     9361 aaatcggcac cagcggtggt gctatcacgt ctatctcgcg taagctaccc ccgtcggttt
     9421 cgccagaaat cgtgcagttc ggatctgcct cttgccccgg agtagggcca actcctgtct
     9481 cccgctccat gtctggattg cacgggaaaa gcactatttt taagatccct cgacttcgct
     9541 cgggatgaca cagtgcagtc aggatgctct catgaatttg gagtgtggca ttaggtgtaa
     9601 ccgggcatca ggcagtgtgc gtgtgccggt gggctgagtc aggttgatcg acggagacgg
     9661 tgaagtggca ggtctcggtc acataagatt tcgacgcgtc cttttctgcc attggcgtca
     9721 aggcaatggt gacccgttcc ggaagctgat cgagaaccga cggatcatcg agcacatcct
     9781 gcacgaacac aaaggcacgc tcgatgccct cctcaatttc catccgctcg ctctcggaaa
     9841 actgccgctg tcctgtcatt gccgtccatc ctccacgaat cgtgagaggt gcccctgcca
     9901 atggttcctg atatcgctca tgccttcggt cagcacatcg tttcactcta gcattcgccg
     9961 aggggccagg atacgcaacg tcctcctccc ctcgatgacg taccatgggg cgtccatccg
    10021 atccttgttt accatggcgc aggcccgtgt cctcgatgcg tatacctatt aatagctctt
    10081 cggagcacgc acctgacatg gcatcgccgc tgggcattct catcgaaccc gatcaacacg
    10141 cttgatggga aatgaccatg atcatgatcg tctggggcgc ccatcccgac cgcaggggcg
    10201 gacccgggaa gggccgtccg ctcggattac cgctccagta atcccttcac cgcctcccgg
    10261 ccctgcgagg taaggacgag gttgccgtcg tagcggatca caagatctcg gtcgatcgcg
    10321 cggcgaaggg tgcgggtagt cgcctccggc gtccagtgga gcgcggcact gatgtgcccg
    10381 accgcgctct ccgacgcttc ctcctcggtg cgctcgtggg tcgagaggtg catcagcagc
    10441 atgtcgagcg cgaaccgctg gcgattcagc cggttctggc gagccttggc gacgagtccc
    10501 tggctggggg agaagagcag cgcgagcgcg aaggagacac cggtcatcgt cacgataccg
    10561 ccggagaccg acccatcgat ccagatcgcg agccagtagc cgcttacagc cgaaagcgcg
    10621 cccaccatcg ccgagtaggc gatcatctcg atcagccgct cggtcaggag ataggccgtc
    10681 gccgccggca cgatcaccag ggcaacaacg aggatcgccc cgaccgcggt aaacgcccca
    10741 accgtcgtga tcgagagcac cagcatcagc acgtaatgca cgagcaccgg tgagaagccg
    10801 atcgtggcgg cgaggccggg gtcgaacgtg gtgagcttga gttccttgta gaagaggaag
    10861 aggaagccca ggttgatgac cagcagggtg cccatgatcc agagcgactg cgggccgagg
    10921 tcgcgcccgc cgatgaccag gtggtcgaag aacgagaact cgatattgcc gtagaggatc
    10981 gcgtcggtat cgagatggac gttggcgaag aagcgcgaga cgagcaccgt cccaagcgcg
    11041 aacatcgccg ggaagacaat ccccatcgcc tcgccaccct cgatacgacg ggtccgctgc
    11101 agggcttcga cgagggagac ggtcaggatc gcggcgccgg tcgcgccgag ggctccgacg
    11161 agcaggttcg ggctgttggc gagccagtac cccgcgacaa gcccaggcag gatcgcgtgg
    11221 ctgatcgcat cggtcatcat cgccatcctg cgcaggatga ggaacgtgcc gagcagggcg
    11281 cacgccatgg cgcacagcac accggtcagc aggatgacgg tgccggacgt catccctgtg
    11341 cctccggcgc cacggccggg agaccgtttg catggaggtt cctgcgctga cggcgccggc
    11401 gaacccgatc cggaacgagg ccgtgtgtcg gcgcgaagag gatggagatg agcacgatga
    11461 ttgtcagcgc aaggacgatc atcgggccgg tgggaagccg gctcgcctgc acgctgatga
    11521 ccgcgccgct cacgccggca acagcgccga agcaggcggc aagcaccagc atgatgtgca
    11581 ggtggttcgt ccattgccgg gctgccgcac cgggaccaat gaccatggcc gccatcagga
    11641 cgaccccgac cgtctgcaag ccgatcatca ccgcgacgac gagcagggag gtgagcagga
    11701 tgtccaggcc gcgtgtcggc aggccgatgc tggcggcgaa gagggggtcg aacgagagaa
    11761 gcttgaattc cttgtagcag gcgacgacga tggccagcgc gagggcgccg aggatggcga
    11821 aggtgacgac attcctggcg acgagcgatg cggcctggcc gaagagatac cggtccaggc
    11881 ccgcctggtt cgcattgttc tgctgctgga tataggtaag cagcaggatc cccacaccga
    11941 agaagacgct gaggatcacg ccgagagtgg tgtcctcggt gagcttggtg tcacggacga
    12001 cgcgcaggag cagcatggtc ccgagccaac cggcgatccc ggcaccaatc agcaggatga
    12061 tcggcgagcg ggagttggtc agcataaagg cgacgcagat gccgggtagg gcagcgtggg
    12121 agagcgtatc cccgagcagt ccctggcggc ggagcacggc gaaggagccc agcaccccgc
    12181 tcacgatacc gagaagcatc gccccgagcg cgacgttccg cagggtgtaa tcgaagatca
    12241 ggtcgtgcag caggctcatt gtgccgtggc ctcgtgctcc cgcagcttca gcaggttcgt
    12301 gttgccgccg taggtcttcg ccaggttgtc cgccgtgaag atctcgcgga agggccccga
    12361 ggcgatcttg cgcacgttca gcagtaccag ccagtcgaag tactccggcg cggattccag
    12421 atcgtggtgg acacagataa cggtcttgcc ctggctccgg agggtctgca gcagggtgac
    12481 gatggcgcgc tcggtctggg catcgacgcc gacgaaaggc tcatccatga agtagagctc
    12541 ggcgtcctgc acgagcgccc gggccaggaa gacgcgctgc tgctggccgc cggagagctg
    12601 gctgatctgc cggtgcgcga aatcggccat cccgacctgc tccagactgt gcattgcccg
    12661 ctcgtgctcc gccttgccag gccgcctgaa ccagccaagc tggccgtagg tgcccatggt
    12721 cacgacatcc agcgcgctgg tcgggaagtt ccagtcgacg cttccccgct ggggcacata
    12781 gccgacgagc tggcgctgct tcgcggatgt gtgaccgaag atctcgacgg tcccggcggc
    12841 aaccggcacc agcccgagaa tcgccttcag catggtgctc ttgcctgcgc cattcgggcc
    12901 aatgaccgcg gtcaaggtgc cggtcgggat tgtcaggtcg acatcccaga ggaccggtgc
    12961 atcgcggtag gcgacggtca ggtcgtggac cgtgacagcg gcggtctcat cgcggatgac
    13021 aggaggggac ggccgtgaaa gggtgaggtt catacgatct ccattgatgc tgcaagtgtc
    13081 tcgccgtcgc caagcagcgc ggtgacgatc gtggtgacgt tatggcgcac catgccgatg
    13141 taggtgccgt cggcggtgcc ctcttcgccc atcgcatcgg cgtagagttc gccgccaatg
    13201 gcgacatccc agccgcgggc gcgggaggca gcctgcacgg cctcgatggt tgcgggcggg
    13261 acggatgact cgacgaacat cgcccggatc tctcgctcag caagaaaatc cgccagctcc
    13321 tggacatccc ccgcgcccgc ttccgatgcg gtgctcaacc cttggagacc gcgcacttcg
    13381 aacccgtagc ggcgtccaaa atagctgaaa gcatcatggg ctgtcaccag cacacgttgc
    13441 gattccggaa ggcgctcgac ctgctcgaag acccacgcgt tgacctcgtc gagcttggcg
    13501 atatagcgtt ccgcattctc ggcaaaggca tccgcatggg ccgggaacat ctcgatgagg
    13561 gactcggcga tcgtctccgc gacgatccgc cagagcgaca catcgaacca gacgtgcgga
    13621 tcccaggtgg ggccttcgcc caccacgatg agccgctcct cgggaatgct ctccgagaca
    13681 gcgacggtgg gctttctgcc ggaatcgctg atcttctcga aggtctcact catgcggccc
    13741 tcgagatgca gccctccata cagaatgaga tcggcattcc cgagctggat gatgtcgccg
    13801 gcactcggct tgtagaggtg agggtcgatt cccggtccca tcaggccatt gacacggaca
    13861 tgctcgccac cgatgttgcg ggcgacatcg gtgatcatac cgatcgtcgc cgtgaccatt
    13921 ggtcgcttgc cgtcaccgga ggcagcagga ttgttggtga agccgacgcc ggcagcaacc
    13981 gcacccacgg tgatgagagc gctggcgatc aggttgcggc gagagatggg cgtgcgagtg
    14041 ttcagggaca tggtccgttt cgattgtctg gtcagcgtca ttcgacgagt cggcgcaagg
    14101 tttagccttg gctaaaccct atggtacaac gattgtcctc gtttgtcgcg tggagggtcg
    14161 gcaagcattt ttagttcgtc gcatcgtcgt catcctgagg ctttagctga agcattcccc
    14221 ggcggaaccg atcctcaggg catcgtggcg acagcgtttg cccctgcggc aatgcatccc
    14281 ggctcccgcc cattgggtcg gcgcggaatg atgcgcgtgg ggtagggaga gccttgtgtc
    14341 tgccccgttt tctgtcatta atgatgggtt gccgtttcgg gtcgacacga aggaagttgc
    14401 ctccataaaa aatccatcgt gcctctcacc gcctgcggta cgatctctta aagaaacgaa
    14461 gacactctcc acgatgggac aacgacagat ggcaacaacc cgctttgcgc agaatgacct
    14521 cgctcgcctg agttacgagg tcgccggtcc ggcccacggt ccggtcgtgg tgctgctcca
    14581 cgcgacgctc ggggaccgta tctccctgcg ccccctgcgg gagcgactgg aggggcaggc
    14641 gaaggtcatc ctcgcggatg cgcgcggcca cggtgccagc tccggcctgg ccgggaggac
    14701 gctctcgagc accgatttgg cgaacgatgt cgtggcgatc ctggaagcag aagaggtgac
    14761 cgatgcggtg catctggtcg ggcatgggca gggagcgatc tcggcactgg agctcgccca
    14821 ctggagtccg gaccggattg cctccatggt cctgatcgag ccggatgcgc tctcgatcct
    14881 cgaaggcgag gccgatgacg cggtgagcga ggcacgagaa ggagcgagga aggccaaccg
    14941 cgaggccgcc gatcactctt acaagggact cgcggacaag gcattgaacc ggtatctgga
    15001 tcgccgctgg ggccaggggt ggcaggatcg gctgacgaag ccgcgcctgg cggcggtgcg
    15061 acgcagtgtc caggcgctga gtgcctccct cgacgctgaa gaacgattcc ggatcctgcc
    15121 ggagcatctc gaggccctgg catccccggt tctggtggtg acaggagagg attcgccgga
    15181 ggccgagcag cacatcgcgg accggatcgc gtcgtggatt cccgggtcag atcggctggt
    15241 ggtcgagtct ctccccggcg gcgcaccgtt cagcccgagc gatggcggcg cggccggccc
    15301 gatcgcggat tgggtgctcg cccggttcgg gtgacaatgc gcctgtaagg gtcgactcca
    15361 tgtcgaccct tacggaactt ctcgtcattt aggggacgca gtccgatgaa acccatgcga
    15421 agcgacgtaa cgctaactga cgttgaacct ctggagaggg atttcggggt cgaatgactc
    15481 gcgcgaaggc catgcttgcg agcatcgctt cgcgccggat ccacaccggg ggaaccgtat
    15541 gggcgagttg catattcgcc caattgaccc caagtcttac tgtgggcgca gcccgttcag
    15601 cagcatatcg acgatctgcc tggcgagggc ggcgtccggt ggcggagcat caggctcgaa
    15661 gcgggcaatc tgcatctgtc cgacaaaggc ggtgaagcag acccgggcaa tgaggttggg
    15721 gttgccgggc gcgatctccc cggacgcgat ggcgtccttg atcggcccct caagatacgt
    15781 ccatggccgc tggtaggtct tccagaaggc ggcctggcgc tccgggctca cgtgctggtg
    15841 gaaatccccg aagagacggt tgagatcggc ccgctccgag ctgaagaggt aggtcgcgaa
    15901 ggcggtaagt cgctccccga gtgactggcc ggtattcacc gtctctgcga ggcccgcctc
    15961 cgagcgggcg aaaccgctct gcattacctc gacaaagagg tcttccttat cctgaaagtg
    16021 gtgatagagc gtcgccttgg tcaccccggc cgcatccgcg atctgctgca tcgagacgcc
    16081 cgcgaacccg agctccgaga acctccggaa cgattcggtc aggatgcgct ctcgtgcccc
    16141 cgtaggcgtt gctgccgaaa cctctgtcac ggataccctt cctgaagtcc cacattgacg
    16201 ttagtagctc tcgttagtat agttggtttc tgcctcccta ccaaccggtt ggtaggttcc
    16261 ctaacagaaa aggatcgacc tcagttcatg ctcgatctac tcagaacacc acacgtctca
    16321 acacttcagg agatcaccgc gtgaagcagc aacctgcgac agggatgccg gcatcggccg
    16381 cgtcgggccc cgatcatggg ccccagtatc tctcccactc ccagatcgtc ggcgtgatga
    16441 tcggcatcat gtccggcatg ttcctcgccg cgctcgacca gagtatcgtc ggtgtcgcac
    16501 tgccgcgcat caccagcgac ctcggtggcc tggagcatct cgcgtgggtg gtgacggcct
    16561 acctgctgac ttcgacagcc agcaccccgc tgtggggcaa gatctccgat ctctacggcc
    16621 gcaagaggat cttccagacg gcgatcgtga tcttccttgt gggatcggtc ctctgtggct
    16681 tggcgcagaa tatgcctcag ctcatcatct tccgtgcgat ccagggaatc ggcggcggtg
    16741 gcctgatgtc gatcgcgctg gcgatcatcg gcgatgtcgt tccgccgcgc gaacgggggc
    16801 ggtatcaggg ttatttcggc gccgtcttcg gtgtctcgag cgttgccggg ccgctggccg
    16861 gtggctggct gacggatgcg atcaactggc gctggatctt ctatatcaac gtcccgatcg
    16921 gcatcgccgc gctcgtcatc acctccatct cgctcaagat gcccgtcatg cgccgcgacc
    16981 acaggatcga ttacctcggc gccggcctgg ttgtctcggc ggtctccttg ctgctgctct
    17041 acgtgaactg ggccggcgat cactacggat ggggcgaccc gcgcgccctg gcactgctga
    17101 tcgcgtcgat cgtgctcacg ctggccttta tcgttgccga gaaccgagcg gtcgagccga
    17161 tcctgccgat gcggctcttc ggcaaccaga tcttctcggt ggggaatgcc ttcggcttcc
    17221 tctccggtgt cgcgatgttc gggggcgtga tcttcctgcc gctctacttc cagggcgtga
    17281 tggggatgtc gccgacgcgc tctggactgg cgatggtgcc gatggtggcc ggtatcctca
    17341 cgatgtcgat cgtctccggg ctgctggtta cgaagactgg ccggtacaag attttcccga
    17401 tcctcggcgc cttcctgatc atcgttgggc tgctgctgct cagccagctc tcggtggaca
    17461 cgccctactg gcagatcgcg gtctacgcct tcgtcttcgg gctggggctc ggcttcacgc
    17521 tgcagacgat cacggtggcg gtgcagaacg cggtgccgtt ccgggacatg ggcgtcgcca
    17581 cgagctcgct cacattcacg cggtcgctcg gttctgccat cggcacggcg atcttcggcg
    17641 cgatcctgaa cagccggctg gccactcacc tggtcgacgc gatgggggag gcgggtgtcc
    17701 agccgggtca gctcagctcg gaagcgaccg gcgacatcca ggccatccag aacctgcagg
    17761 agcccgttcg tggcctggtt ctcggtgcct acaccgatgc gctgaccgat ctctttcttt
    17821 acgccgtgcc gttcgtcatc gtggcgctgg tcgtcgcgct cttcctgaag gagatcccgc
    17881 tccagagtgg cgcggcaccc ggccccggcg agcccaccgg caatcctcgc agcgccgaag
    17941 agacgatccc cgtgatgggc ggtcactgac ctaagccatc ggtagccccg gcccggaaac
    18001 ccgtcattca gagcggagca aaggctcgct cacgccaagc caagttggct gagccgccaa
    18061 gaagtgggag caagcctttt tcgccctgag ggctcagcca acagaatcct acgcgaagcg
    18121 cataggtcgc aggaatactc cactgctcgg tgggtactcg caatcgtgtt cctgtaaccg
    18181 tcgcgccctt tcacaacaga tccgtcattc agagcgaagc gatgaatccc aggcgaagcc
    18241 aacgtcgacc agccccagcc gtgagtgaac cgcgctaggg gcagtcagtg tgtgttgggc
    18301 accgcttcgc gtaggattct tcgacacggc tccgctcact acgttcgcgt ttccggcgcg
    18361 tatgcccgca ttcgatatcg acttgcgggc aatcacccgt acgggtcgac tccatgtcga
    18421 ccccatattg ttcctagggg cgacccactc gctcaaccga cccccatccg ctacttcccc
    18481 gccaaggcct tcacaatcgg cgcgaactcc tccatcatcg gctcgaagac cacgaagtag
    18541 gagacaccca cgcgctcccg cacctcctgc atccgcgcca cgatctcctc gaccgacccg
    18601 atgtagacca tcggcgagtc gaaccagccc atgtcctcgt ccttcagctc catctggaca
    18661 cgacgccgct cgatctccgc gtcccggtcg tcggtgacgc cgaacatctg gaccagaatg
    18721 ttgagctcga tctccgggaa ccgttcccct gcatgggcgc ggatggtctc aaccttggcg
    18781 gcgaaggcct ccatgctgat ctcgttgtcc gcgaacccgc cttccgccag catgctgggg
    18841 aggagcccga tgatgtcccc gtgctctgcg ctcagcttca gcatcctgtt gccaccggcg
    18901 cccatcatga tcggcgggtg gggctgctgc accggcttcg ggtggccggt gtactcctga
    18961 aaggtgtagt gctggccctc gaacgagaag ggctcgttgc tccagatgcg cttgaagacc
    19021 ggcagcgcct ccgcgaggcg gctcacccgc gtgccgggag gatcgagcgg gatgccggtc
    19081 cacgggtact ccggcgggaa cgatccgccc gcaccaatgc caaactccat gcggccaccg
    19141 gagaggacgt cgagtgtcgc ggcctccatg cccagcaggg cgggatgccg caggtcgttc
    19201 tgccagacca gggtgccgat gcggatcgtg ctcgtcacct cggcgacgga ggccagcgcc
    19261 ggcccgatcg agaactggtt gccaaagtga tcggccatca ggaagacgtc atagcccagg
    19321 gcctcggcac gccgcgcggt ctctcgccac tcgtcgccgg tgcgcgttcg ggtgatctgc
    19381 acaccgaagc ggaaaggttt gtcattaaag gacatgagtg attctctcct ggttcattgg
    19441 caggacgtat cagggaaaga acgatagggg agggacgggt gttctggcaa ttcagcatct
    19501 tcataggggc cgtacctgcg agggttgcat gataagccct cggtgacggc cagaagcgaa
    19561 gcgaccccat ggccgagttg gcagcaccac cgagaagcgt aagcgagcct tttttcgcct
    19621 tacgggctcg tccgggaatc cccgtgcgaa gcacgtccaa tcgagcacca tggggccatt
    19681 aattgccgca cctcgtcatg gtttggcaca atggtcgagc acccggcatc ggcccgggat
    19741 cattgtcatg ggcgcggcac atcatcgccc tggcgaatga cgcgctgctt cgctgctgga
    19801 gatcacggtt tcatgtcctc atggcaggtg tcctcaccgt cccacaccac atccacatcc
    19861 tatggcggcg aggcggaggc taccgcggca gcgatcactg actcatccgc caacgcaaac
    19921 gcggctcacc ccctggctcg cgatccccga ggatcgtcgt tgccgcgcta gcccgcgttt
    19981 ggagaatccg ggcggtgtcg ggagacatga caccccctct ggagccacga ccatgacccc
    20041 tgtgaacgac ctgggtatcc cctggcagtc gggtatcgat atccggcgca agttcgttaa
    20101 gttctttgaa tccaagcagc atcttcatgt cccgagctcg tcgctcgtgc cgccggccga
    20161 agaccagtcg gtgctgctga cgacggcggg catgcagcag atgaccccct acttcctcgg
    20221 gctggagcag cctccggcgg tccgcatgtg ctcagtccag aagtgcttcc gcaccgtcga
    20281 tatcgacgag gtcggcgacg agagccacag caccttcttc ttcatgctcg gcaacttctc
    20341 cgtgcgcgac tactttaagg aagaatccct cgcgtggtcg tgggagttcc tcaccgagtg
    20401 gatgaagatc cctgccgaca agctctaccc gaccgtgcac ccggaggacg aggaggccta
    20461 cctgatctgg cgcgaccaga tcggcgtgcc agaggagcgg atctccaaac tggaggacaa
    20521 ctggtggtcc gccggtcctg tcgggccgaa tggcccggat tcggagatct acgttgaccg
    20581 tggcgagcag tggggctgcg ggcagccgga ctgcggaccg gggtgcgatt gcccccgcta
    20641 cctcgagacg tggaacaacg tctttatgca atacaacacc accgcggatg gcgtccgcac
    20701 gcccctgtca aagccctgcg tcgataccgg tatgggcctg gagcggctga cgctgctcgt
    20761 acagggcgcg cggacgatgt atgagacgga cttctaccag ccgatcatcg ccaaggccag
    20821 cgcgctcgcg ggcgtgacct acgacgcgga cccgcagatc gacaagtcgc tccggatcgt
    20881 cgccgaccat gtgcgtggct ccgtcttcct catcgccgat ggcgtgctgc ccggcaacga
    20941 ggggcgcgcc tacgtgctgc gccgcgtact gcgcaaggcg atccgccacg ggcgcctgct
    21001 cggcatcgaa caggccttca tggccgatct cgccgaggtg gtgatcgagc agttcaggga
    21061 ggcctacccg gaactgaccg accgccggag ccagatcctc aaggtcatcg cctacgagga
    21121 ggcgagcttc ggcaagaccc tcgcctcggg catgaaccgc ctgcaggtgc tcacgggcga
    21181 gctccggcaa cgtgggcagc gcgtgatccc gggcgacgag gtctttcgcc tctacgatac
    21241 ctacggcttc ccctacgatc tgacggcgga gcttgcctcg gaagaggagc tgaccatcga
    21301 cgaggagggc ttccgcctgg cgatggagcg ccagcggcag acgagccgca gtggcgccgc
    21361 attcaggagg gagggccgcg agcgggcgga tctctatgcc tcgatccagc cgcgcacgga
    21421 gttcctcggc tacgaagcga cgcgggccga cgcgaggatc gtcgcgatcc tcggcccgga
    21481 cgggaatctg gagcaggcgg aagcgggcga gcaggtcgag atcgtgctcg accgcacgcc
    21541 cttctacggc gagtccggcg gtcaggtcgg cgatactggc gagatccgga cggaaaccgg
    21601 cctggttaat atcgacgaca ctcgcaggcc cgcccccgac ctcttcgtgc atcggggcat
    21661 cgttgaggag ggtttcatcc gtgtcggcga gtccgcgacg gcgcaggtcg actccgagcg
    21721 ccgcctgcac atccgccgca accacacagc gacccacctg ctgcacaagg ccctgcggct
    21781 ggtgatcggc gacgaggccc accaggccgg ctcgctcgtg gcgcccgacc gcctgcgctt
    21841 cgacttcacg gcgctggagg cggtcgagcc ggagcaggtt cgctccatcg tcgagatcgt
    21901 caacaacgag gtcacccagg acatccccgt ccagaccacc gtgatggacc agaaggaggc
    21961 cctcgccggg ggcgcgacgg cgctcttcgg cgagaagtac ggcgagaagg tccgcgtcgt
    22021 ggagatcggt gatttctcga aggagctctg tggcggcacg cacgtggacc gtactgggga
    22081 gatcgggccc ttcgttatca cctcagaggg cagcgtcgcc tccggtatcc gccggattga
    22141 ggcgctgact ggcaatgccg cggtcgagcg catgctcggg cagcagcgat tgatcgagga
    22201 actcgggcgt gacctgaagg tgtcgtggtc cgaggttccg ctcagcgtca aggcgctgca
    22261 ggagcgcacc cgggcccagg agcgggagat cgagaagctc cgcggccagc tggcgggcgc
    22321 gaaatccggc gacctgctcc agaacgcggt ggaggtcaac ggggtcaaga tcttgtcgag
    22381 tcgcgtcgat gtgaccgaca agggcggcct ccgccagttg ggtgaccggc tgcgcgattc
    22441 cctacaatcg ggcgttatcg tgctcggtgc cgtcgtggat gagcggccga gcctgctcgc
    22501 gatggttacc ccggatgtgg tatcacaggg cgtgagggcc ggagacatca tccgccagat
    22561 cgcgccgcac gtcgacgggc gcggcggtgg ccggccagag ctcgcggagg cgggcggcaa
    22621 ggacccggag ggcatcgatg ccgccctggc ggcggtcgcc gggatcgtga ccgggcagac
    22681 gggcgcttga tgagcacgga tcgccagcaa ccgccggtca ggatcaccat cgacccgaac
    22741 gattcactcc ccgtcatcct gcagcagctg cgggagcacg gcgggaagtc ggtggtgatc
    22801 gatatcccgg accattgccc ggtgttgctg accgcaaccg agtttcggac catgaaggag
    22861 ctggccgagc gggagcgcat ctcgctctcg ctgcgcacgg acgaccggct ccgtcatcag
    22921 ctgtcatcga tgttcggcat caagaatgtg ggagatgcgg gtgcgcagtc gccaaacgac
    22981 ggctggcgcc cgcccccgac gatgctggga tcgccccggg ccttcggcac ctggaacaag
    23041 aacgacgatg aggacgacgg gatagtcgag cttgagccag gtacgcgttc ccgtcggcga
    23101 cggcgtggcg aggtcatcga gaccaacacg tacacgaaac gcagcgacga tgatgagggg
    23161 atcagctcgc tcgaatacct gaacgagccg aaggagctcc tcaccgcgaa gaacatcggc
    23221 cgcatcgttg gggtgctcct ggtcgtggca ctgctcctgg ccgccgcggg ctggtactac
    23281 atgcccgctg ttacggtcga tgcaacgctc cgccgtcagg acgtttccaa tcagctcctc
    23341 tacagcgtgg ccgcccccgg cgcgcagctg ccgagcgaca tcgccttcac cgcggacgcc
    23401 acccagggct ccgcgacggt caatttcacg atcaccatcc cgaccaccgg tgttgagcgg
    23461 acgccggagg ggacggcttc cggcagcgct ctgctccgga acccgggaga ggcaccggtc
    23521 accgtccctg cgggaacaga gctttccgtc tacgccgggc ccacgtttac caccaacgaa
    23581 gcggtcgagg ttccacccgc cgcgaacggc gtggcggggg aggcctccgt gggcgtcacc
    23641 gccacggcac cgggatctgc gggcaatgtc gaggctggcc tgctcaccgg ccgcctggcc
    23701 gatctcggtg tcttctacag caaccgcgag accccgatcg agggcggcac cgatatcgag
    23761 gtcatggtgg tggcacagga ggacatcgat cggctccggg aggaggtggc aaccaatctg
    23821 gaccgcgcgg cggcagaggg ctggatcgcc cagctgccgg aagggcagtc ggtggtcgtg
    23881 ccatcggtgg ataccggcga gccaacctac gagattaccg cccaggttgg cgagaagctg
    23941 gacgagatca ccgccactgg cacggtcgac gcgaccggcc tcgtctttga cgcggcgacg
    24001 gtcgagcagc agacgctgga gttcttccgg actgccctcc aggaagaggt cccggagggg
    24061 tacgcgctcg atcccgagag cgtgacgcta agcgagcccg cggtgatcgc cgaggccccg
    24121 gataacgtcc agtaccaggt cgaggcatca ggcttggccc atgccgtcta cgacgagtcc
    24181 gtgcttgagg acctgtcaga ggacctcgcc ggggaatcgt gggatggggc ggaatcgacg
    24241 ctcgctgggg tcgaggcgtt cgagtcgtgg gagatgtcga cgagccccgg ctggtgggcg
    24301 cgacgaatgc cgcagacggg ggatcgcatc acgatcaatg tcgagcaagc gccggctgca
    24361 tcggatgccg gggagccggc accgagcccc tcacccggcg aaggagacgg atcgtgaaga
    24421 tgggtgagcg cctgctgggg ctggatatcg gtgggcgccg gatcggcgtg gcggtcagcg
    24481 atgagatggg catgatcgcg agccctgtcg cgatgattgc gcggcagtcc gacgttgccg
    24541 ggcaactgaa ggagctggtc gctcgctaca atgcggccaa gctcattgcc gggttgccgg
    24601 tcgggctttc tggccgggag gggccgcaag cggctgaggt ccgcgcgtac accgatgcgc
    24661 tcgcgctgga ggttggggtg ccggtggaat actgggacga gcggctatcc acctcaattg
    24721 ccgaacagtc gctgatcgca caggggacac ggcgcaacaa gcgcaaggaa cgggtggatg
    24781 cggtcgccgc ggcagtgatt ctccagagct atctggaaaa ccagcgctgg aagctctcga
    24841 ccgggcgtcg gtgatccggg agaagagcgc gtgtcatgaa acaggggttt cgaccggcaa
    24901 acgggaccaa cgtgcagggg gataacatgc gttccctctg gctcccaccc gcctcacaga
    24961 cccgttgaga tgcaccgtct tgcatcgtga tacagtgtgg gacagcatgg acgacattgt
    25021 agcgaagggg agtccttcga tggggcaaat caaccgatcg tcctgagcac gcatagtcgg
    25081 cccaggatga cgcgaccgac cggatagcag gccaatcgtg gtcgtgcggg agagagacat
    25141 gaagaagctg atcattgctg tggtccagaa cgaggatgcc gacgctgtcg tcgatgcgct
    25201 gctcgaggag gagttccgcg cgacccgcct ggcaagcacc ggaggtttcc tgcgccgcgg
    25261 caacaccacg ctgatgatcg gcgcggacga ggagcaggtc gacgccgtgc ttgacctgat
    25321 ccggcggcac gcgcacgcga ccggggatgc ccagagcgcc gggcagtcac agcccgccgc
    25381 cgcgaccgtg tttgtcctcg acctcgagga gtaccagcgg ctctagtgtc cgccgggaaa
    25441 agcgatgcta acgaaagacc ccgggcaaaa cgcccggggt cttgtttgcg tggtgtatta
    25501 cgtagacgca ttcacgagcc gggcaggact gtatcccgaa gggtgtcaac tcgctgtcgt
    25561 gaagcagcac gccatgcgcg tcaccaacgg ttctcgcaca acaagggctc gcttcgcttc
    25621 tcgcgcccca tgctcggcca gggtgcgggg ctacaggaag ggggcgttga gtccgtgacg
    25681 ctggctacat ccgctcggct ccggtgaggg agatcaggcg ctcgaagtaa tcggggaaag
    25741 tcttggcagt gcattcgggg tcgaggatgc ggatgccgtg ggcccggagc gacgtgacac
    25801 cgaagctcat cgccatccgg tgatcgtcgt aggtctcgat gtcggcaggg gtgacgggac
    25861 gcggggtgat cgtcatcccg tcctcgaact cctcgacctc ctgcccgagc tttcgcagct
    25921 cggtggcgac ggcactgatg cgatcggtct ccttgatgcg gttgtgggca acgccccgga
    25981 tcgtcaccgg tccgtcggca aagggcgcga ttgctgccag agtctgggcg gtgtccgaga
    26041 tctcggtcat gtcgaagtcg ccgcccttca gcgcgccatc ggtcggccca cgtacgatcg
    26101 tctccgactc cctcatctca acctcggcac ccatcgcctc gaggacatcg acgaaatcga
    26161 gatcgccctg cagcgagtcg gtgcccaggc cttcgacgcg gacctccccg ccagtgatcg
    26221 ccgccgcggc aaagaagtag gaggcgttgc tggcgtccgg ctctatatgg aagaggcgtc
    26281 ccccataggc ctgtcccggt tccacccgga atgtctgcca ggtctcgtcg tcgatctccg
    26341 cctcggcccc gaaggctgcc atcacatcga ccgtgatctc gatatagggc ttcgagacga
    26401 ggtcgccatc gaccttgatg gtcataccct gctgggcgta gggcgcggcc atcagaagac
    26461 cggtgaagta ctggctgctc ttgtcgccgg ggagggtgac cgcgccgccc gggaggccct
    26521 ccgcgacgat cctcaccggc gggcagccgg tgccttcctc actggcggcg ttgacgccga
    26581 gttgatcaag cgcgtcgagc aggggagcga tcggtcgctc gcgcatgcgg ggaacgccgt
    26641 cgagacgata ggtaccgtga ccgagcgcga cggcggcggt cagaaagcgc atggacgtgc
    26701 cggagttgcc tacgaagagg tccgcttcat ggacggggat cttgccgccg catcccacga
    26761 ccgtgaatga ctctgcctgc tggtctcccg taatccggat cccaagctgg ttgagggcgg
    26821 cggccatgta gcgggtgtcg tcgctgaaga gtgcgccagt gaggattgat tcgccgttgg
    26881 ccagtgccgc gatcaggagt gcacggttcg tgatgctctt ggagcccggg atgttggcgg
    26941 tgccgacgac cggccggctg acaggggcga gctcgagcac gttggcgcga aaagacgagt
    27001 gggaatagaa catcgacatg cggtagcact ccggggatgg gacagtggcc ggtcaggcag
    27061 aggatctgcc cgcaagatgg gtcaactgtg gcggaaccac tgccccgcgt caatgtggtg
    27121 cttgacagtg ttgcatcctg ggagcatgat ggggctaccg gggccctctc atccgcaatt
    27181 catcctgtcg tacggcatcc tctagcatga agcgagggcg gccatgtcgg ggctctttgg
    27241 ttcgaccatc cttgaggtgg tgctgggact cgcattcatc tataccctcc tgagcctgct
    27301 gtgttcgagc atcaatgagc tcctcgcggg tttcctcaag ctgcgagcca ggaacctgga
    27361 gagggcgatc cagaatatcc tgtgcgacga agacctggta cgggaagttc tcggccaccc
    27421 gctgatcaag gccatgggga gcacacgcgc cgagacgact ggcgttaagg taggtgcagg
    27481 ccaggggacc cggaaggatt ttgcaggcaa gcccagctac atcccgtccc ggacattcgc
    27541 tgtcgccctc ttcgatacca tcactccccc caccggtcgc tcgatcacgg tcggcgatgt
    27601 ccagcgggca gcggaagaga tggctggagc ggtcgagaaa cgggctgcgg acctgacgcc
    27661 cagggagacg atcgggcgag cactgtcgag cctgatcaat gaaagccgcg acccgcagca
    27721 gctcttcctg acggtcgacc aagtcaagga ggtcgtcaac agcctggcag atccctcgct
    27781 tgacgacgaa accaggcaga agttatccgc ggcagcaacg ctggacgatc tccgccgcct
    27841 gattgagcga ttccccgacg acaccatacg tgggcgactg atcgatatgc tcgccgaaag
    27901 ccagacgggc ctggaccggg cgcggctcag catagagcgc tggttcgacg acgccatgga
    27961 tcgcgcttca ggtgtataca aacggcgcac gcagtggtgg ctactcatga tcgccgctgc
    28021 ggtcaccctg tgtatcggag ccgacatgtt ccggctctat gacagcctcg cgaccaatcc
    28081 cggcttgcgg accgcgctcg ttgcccaagc ggagcagcaa gccgcggctc ccgcgagtga
    28141 cggggtgttg ccgtcagaga caaacgatgg tgaggtgagc gataccccgt ccaccgacag
    28201 cgcggagggc tccctgcccg cgccgagcgt aggtgagata ttcgacgaac tcgatcagac
    28261 gagcttgccg tttggttaca gtgaccggcc aggcatggat ggtgaggagg cggacgcacg
    28321 tgactggttt cgctggctcc tcacgaagat ctcagggctt ctcgtgacga tgttcgccat
    28381 cgctcttggc gcaccgttct ggtacgacac actcaaccgg gtctccaaca tccgcgccgc
    28441 cggcaaacct ccggcttcaa cggacgcttc tgctgcgcct tcccgctcga cgtaacagag
    28501 taggcgtcag cgccaaactc cggtcagcag atgacgttga cctgatgtgt tcattgaatg
    28561 gaaacactcg cggcgatgta tgaacctgag tgagcctcgg tccatcgttg ccggggtctt
    28621 tcttggatca cttaactgtc accgagggca gagacgatgg tattggtgtt gtaggtcatc
    28681 atgccgatgt agctgctgcc gtcggaacct tcctcgccga gcgcgtccgt atagagcgtg
    28741 ggggcgagct cgaccccggc ctcgtcggcg atcgactgca tcagggacgg gttgaccacg
    28801 ttctctgcga agattgccgg tacgtccgcc tcctggatat cctcgacgag tgtcgtgatc
    28861 tctttcgctg acgggtctcc ggattcgctc gaggatgagc caagcgccgt gccgatgatg
    28921 tcgaaaccgt atgccttggc gtaataggcg aaggtatcgt ggctggtgac gagctttcgg
    28981 ttcgcttccg ggatagtggc cacctgctcg cggatggcgc cgtcaagctc cttcagttgc
    29041 tcgatatagg cctccgcatt ggccctgtag gtatcggcgt tgtccgggtc cacctcggca
    29101 agggccgtgc cgattagctc aacggatgaa atagcattgc tgacatcgcc ccagatgtga
    29161 gggtcaccct cgtcctcatc atgattctcc tcgtcttcgt ggtcggcacc gagaagcgtc
    29221 atatcccggg tcacctcgac gtaggcagct tcagtgccgg atgcctcgat catgtcggcg
    29281 agccagggct cgaacccgga accgatcgcg aagatgatgt ccgcgtcggc aatacttgcc
    29341 accgtagctg ggtcgggatc gaacgtatgg gcgtcgccac cagccgggac gattgacgta
    29401 acgtcagccg agtcgccggt gacattgctg acccagtccg ccaggatgct ggacgaggcg
    29461 acaaccttga tcggatcgcc atcagcggca ctggtcggtg acgaaagggc gaggagaccg
    29521 aacagaagcg cgaggatgat tgccacggag gccctgcgga gcaggggcat ccctaccaat
    29581 attcgggact gggacataaa acggcgaatc cttcactggg gcagtgccgg ccagcatgag
    29641 cccgacggca agatacaagg caaatggggc acacaaacag gaacaagatc ctccaagatg
    29701 ataccgagta tcagtatatg gacgctgatc accctcgtca acgaggcgcg gtcctgctca
    29761 aaccgggaac ccgtcgacgg gttcccgtcg ttgtaccaat ggaacatatg aaccggcgcg
    29821 agcatcccgg agggtagatg cttgccacgc tgaccaggcc aggggatcaa tcatgctgac
    29881 atcacttagg aaagccgtgc ttgccacgct ggcactgtgg ctcatagttg gcggcacctt
    29941 cggtgcttca ccggcaagcg cgaggcaatc ggaggacgaa gaagaggagg tggtgcgcac
    30001 cgtcatcgac ctcctgcggc tcgaaggtca aggtgacctg gaccgcctct acgatgcgat
    30061 ggcgccggaa tcccgcggga tcatcccgcg ccaggccttc gtgaactggt actcagatga
    30121 ggaccggacg gtgcccgccg atgagccgga gatcgagtcg atcacctttg acgactggga
    30181 atcggatgcc accggcgacg agtacgaaga tgtcgcagtc gtggagtatt ccgtcgaggt
    30241 tgaggctggc cgcgacgaag acatccgctc cgcggagatg attctctgga acgatgggca
    30301 ggtctggcgc tggttttttg ccgacatgga ggtggacgcc gaggacgttg cggaggagga
    30361 cgagtggacg gtagactacg agactccgta ccaaaccgag atgtaccaga acctcgatat
    30421 gttctgggcc cagatcttcg acaatgccgg tctcgcgtac catccaccgg tggatatggt
    30481 aggcgtgctg gtcgagccga cgcgcactgg ctgcggcgtc gagaacgaca tcacgcagat
    30541 ggccgtctac tactgcaccc tggatgagac gatctattac gaccccgctt tccgcgatgc
    30601 gatggtcgag aacatcggtg actatgcctg ggagcatgtg atcgcccacg agtgggggca
    30661 ccacgtccag aatctgctgg gtctcacgac cgcgatggac cccgagcttg agggcgggca
    30721 gtacacgatc gagcatgagc ttcaggccga ctgcctggcc gggatgttcg ggcaggacgc
    30781 tcgagcccgt gggcttatca gaagccggga cctgaacgag gcgatcgata tcacgggcat
    30841 cgccggagat gccgccggta cctcgtggga tgatcccagc gcgcatggca gcgccgagca
    30901 gcgggaggag tcattctgga ttggttacaa tgacggcttc cggggatgcc acatcaggct
    30961 cgatgtcgaa ggtggcgacg ggaacaaccg atggtgagcc ggaatttccc agggatgaga
    31021 ggcggccact tcggccgcct ctcattgttt gcccagtgct ccgatatcac cagggggcgt
    31081 gcggatcttg cgcgattccg tacaatgtag caaacacgca tcttcgccca aggagcccct
    31141 catgacgatg gcacacaccc agaccgaaaa tcccctggat gttttcactg atgtcacctt
    31201 gccacgttgg gcgctggtcg agcaacggct cgatgacaca tcggtggggg atttcgggga
    31261 ggccattgcc cgggagtttg cgcggcccga gatccggtcg acgattgggc cgggccagaa
    31321 ggtcgcgctg acggcaggta gccgcggtat cgaccggatt gccgaggtgc tccgcgccgt
    31381 cgtcgatcaa gtgagagggc tcggggcgga gcccttcatc atcacggcga tggggagcca
    31441 tggtggcgcg accgccgagg ggcagcggga ggtgattgcc cattacggtg ttaccgaaga
    31501 gaccatgggc gcgccgatcc gtgccagcat ggagacggtg cacctgggca ccatcgaggg
    31561 gaacgtgcct gtctggttcg accggatcgc ctacgaggag gcggacgtcg ttatcccgat
    31621 cgggcgcgtg aagccgcata cctcctttcg cggcccgacc gagtccggcc tgatgaagat
    31681 gctggcgatc gggctgggca agcagcacgg cgcaagctgg ttccatagcc atgggatcgg
    31741 gacgtttggt gacctgatcc cccgggttgc gcagcacacg ttgtcccagg tgaacatccc
    31801 attcgggatc gccctggtcg agaacgggct gagcaagatg gccctgatcg aggcggtgcc
    31861 gcgggagggg atgtatgagc gggaatgcga gttgctggaa attgcccgcg gcatgctggc
    31921 ggtcttgccg ccggtcgcga aggtcgatgt cctgatcgtg gatgagatcg gcaaggacat
    31981 cagcggtgac ggggcagacc ccaatgttgt ccaccgcggc gttgcgaacg agatcgactt
    32041 ttccgaggat cgtccctcta tccagcgagc ggttgtgcgc gggttgacgg aggacaccga
    32101 gggtaacgca tccggcatcg gcatgtttga cttcgccctg cggcgcgcgg tcgaggcgat
    32161 ggatccgatt cccacctaca tgaacatgct gacggccaag gcgccgcaag gcgcgaagat
    32221 accgatcacg gttgagaacg accgccaggc actctccctc gcgatcgctt cggcattgag
    32281 ggtggaggag ggcaccgcga agattctccg catccgtagc acgaagcacc tgacccagat
    32341 gctgctctcc gagccgctgc tggcggagct ggaggggatg gacggtatcg tgagaatcgg
    32401 ggagccgatg gatatcgggt tcgacgccga gggcatgttc aacgcggagt tgtagcgccg
    32461 cagcatcagg aacacctgat acgcaatccg gatgaacaat cccgcacgtg tcgcaaggta
    32521 tcaccccggg gttgacccat tcggcacgct cggggggttg gtgctgcttc gccgatcggg
    32581 tcaacatgga gttgccccct acacgtccat gcattgatcg actgaattcc gtatgatgcc
    32641 cacttctact gccgcccggg catcggcttc gccgggggga ttcttcgcgt tcgcccagaa
    32701 tgacgattgt gtgagggcgt tcagcggccc ggcccaagat cctgagtttg ctgcaggtca
    32761 accgtgtacc tgatggcttc gccggagatc cttcccggtg gtcagaatga tggatgtggt
    32821 ggcgtgtgcc ccgcacaccg gattccctgc taccgcgcca tcgattccgc gcgttggacg
    32881 caatccagca ggcgctggcg gatcgcgatc ggcagatccc gccaatcctc ggggctgtcg
    32941 aggtcgcact caagaccagg agcccttgcg gttttcatct ggataccgag ccggtctgct
    33001 tctgcgtggt gccggtggta gctctggctc ccgaacgcaa aggcgaactg ttgcgggtgt
    33061 gcggaatcga gccggaggaa cagggcattg gtcccacttc catggcgatc cggcgcgatc
    33121 acggcaggag catcgggccg aaccatgctc tccacatcgg cgggggccag tatcgggaga
    33181 tccgccggca ggaccagcat cgcgtcatag ctatgtgtga gggcccagtc gcggccgaga
    33241 tcgagcgcgg cgttgagccc ggtgtgctcg accggctgaa acaggaccgt gtgccgggga
    33301 tccgggccaa tctggcgcaa cgtctcgtcg gactcgcggg tgacgatcag ggttgtgtcg
    33361 atacccgggc atgcgtccag cacgtccagc acatgggtga gcatcgacca ggcgagcgag
    33421 gcgcgctcat gctccgcgaa tatcggggat aatcgcgtct ttgccccgtg cgccccgcga
    33481 atcggaacga cagcgaccgt gcgcatcagg cgcgcgtcca ttccacgtca agaatctcct
    33541 gtgccagccg tccccggtcg gacgtgtctc ccatgatggt ctggcccacg tgcacctgca
    33601 acccctgctc ggccaggggt gtttcgagcg ctgcatcggc atggtcgatg acgatgccgt
    33661 cgataatgcc cctgtagatc tccgcgacgc cgagcggcga cacgtcgacg ccccgggatg
    33721 ccagcatgcg gtcggctggg cccttgagcg cccgtccacc gatgatcggg ctgacggcca
    33781 cgatgggcgc ttcggcgtcg atcagggcct cgcggattcc cggcagcgcc aggatgggcc
    33841 caatgctgac gaccgggttc gaggggcaga agatgatgag agtggcggac gcgatggctt
    33901 ccagaacctc gctcgtcggg gttgccgcct cgatgccctt gaaccggaca tcaagcactt
    33961 cgtcgtcatg gcgccgggcc acgaagtagt cctggaaccc cagccacccg tcggcggtaa
    34021 ggatctcggt cgctaccggg tcgtcggaca tggggagtag tgtcgcctcg atgccgagag
    34081 ctgccgccag ccgggccgtc gtggtggtgt gcgtcgcgcc ctcctgaaga tagtggctac
    34141 ggaggatgtg cgtggcgaaa tcctgatccc cgagcgtgaa ccagggatcc tcaccgtatc
    34201 gagcgatggc gtcgagggtt gcccgggtat cccccgcaat gccccagcct gttgtggggt
    34261 tcgcgagccc ggcgagcgtg tacatgacgg tgtcgacatc ggggctgatg tacaggccgt
    34321 acaggcgaaa atcatcggtc gtgttgatga tcgcgctaag ctgatccttg ggaaggacgg
    34381 cggcgagccc ctgcaccagc tttgcaccac cgacaccgcc cgcgagggcg accacattgc
    34441 catattcggg cacgaaaacc tcatctcgtt acgtggcgac gaagggccgg catgatgctg
    34501 acgatgatac gtgacaggga cgtgatgagg gtgtccctca gccccgttgg gtggttcaaa
    34561 gcggaggcgg ccattgcccg cgggcaccgg tggcatggtg caggagggca gcccgttcac
    34621 ggttcggagg cggggcctct tctcttccgg gtgcctgtcc tggctggtac gatgcgcgtc
    34681 taacggattt ggggcgggcg tggcgacatg ccggtcagga tcagatggag gcggttggtg
    34741 tttcaggatc tgcatatcat cgatgttcac gcgcatttcc cgatcagggg cgatgccagc
    34801 cagggcggga gccgcacccg ggagacggga aggatcgata atccggcggc tgtcgagcgg
    34861 caggagcttc gcacgcagca gctggagcgg atgcgccacc agtggcggat ggcgttcgac
    34921 ttccccgagc cggagacgga gccgcgcagc agcgaggagc aggctgaccg ctgggttgcc
    34981 gagctggaga agtacggcat cgagcgcatt ggtttcgtga ccggccggga caacgatgag
    35041 ctggcgcggg tcgtgaagat gtatcctgac cgcttcatcg gcttcgccca tcacgacatt
    35101 acccggccgg atgccgggga ggagctggag cgggccgtgg gcgagctggg cctgaaggcg
    35161 ctgaagctga tggcgcccag gatcccgatc ctgatcaacg acgcgtcgct ctacccggtc
    35221 tgggagacgt gcgagcggct ggggatcccg gtgctctgtc acttcgggat gttgggcgcg
    35281 gctggcggga tctcgtatca cccgaatatc tcgccggcgg tgctggagcc ggtcgcgcgg
    35341 gatttcccga cggtcgattt catcatcccg cacttcggca tccagcatgt gaccgacctg
    35401 ctcttcctct gctggtcgtg cccgaacgtg aacgtggact cctccgggtc caaccagtgg
    35461 gtgcgatgga tgccctacaa gctgacgctg gacgatttgt tccagcgctt ctacgagacg
    35521 gtcgggccgg agcggatcct cttcggcagt gattcgagct ggttcccgcg cgggtttgcg
    35581 ctgcggtacc tgcaggacca gctgcgcatc tgccggttca tgaacatgcc gcacgagcac
    35641 ctgcagctga tcttcggcgg caacgcggcg cggatcctga acgtgcccct gggcgatatc
    35701 gacgcgagga aggcggctga ggggcaggct ggatagccgt ttaggctgcc aaatgaaaca
    35761 atttggccgg tggcagccta agggagatcg ggggctgatt ttccctgcgg tgacggggat
    35821 ttcttcaggc tgccacatta tccggatcgt ggggtgaaat gtggcagcct gggcggaatg
    35881 tgcttgattg cacgtgcttc gcacggggat tcttccctcc ggtcagaatg acggtgttgg
    35941 cggggaagca cggcgcccaa gatgatcacg atcatttgca ccatgcaagg atgatcgcca
    36001 tggtggtgcg aagcgacggg ccctacttag tatacgtatt gaggacgccg aattgcgcca
    36061 cggcaggacg gattggacgc gtgtcagggt agggacagca ccagtaggca tcgaggacat
    36121 cggcggacac cgggcgaagg gtcgctaccg cttctcgcct gctgtgcagg ctcacccatg
    36181 ggggtcgcta cgcggcattg ggccgaccca ttcgactccg ctcagggcag gctattgctt
    36241 cgcaagtacg gccgctacgg aagggaatcg ggcgaacatg gagttcgccc ctacaccagc
    36301 gaaaaactgc ggcgttttcc accatcattg agaccgcagc gatgggccgc agccggtcaa
    36361 ctgcctatgg gagcgcgccg ggcttctcca gcgctgggtc gttggcagca gtactgcata
    36421 ggacccgacc atttcgccgg gcaagaacag caggaagaag cggggggatc gcggggaatc
    36481 ggcgtcctgt aaatgaccgg gaccgcagcc ctgcaaaaag aaaggggtgc ggtcccggta
    36541 gtgccgccgg ttgtgggtgg cgacatcctt tcggccctca gttagtccag agacggcttg
    36601 agggtgtatc tcggcctcct agaagatgag gatcagaata atgatgatca ggatcagtgc
    36661 tccgataccg atatacatgc atctctcctt cctacttcac ggcgtgtcgt ctcgatttcc
    36721 gtcctgtcag ggacaggttc tggtatgccc ctcatcacga ctcgtgccac cgcctcaccg
    36781 ggtttctcta tactcgcacc ccatacacct acacccaagg ggtcggtggc aatcccattg
    36841 cagattgctg caaagcttat tgccccaacg cttcgctccg cgcgggctat tgcttcgcag
    36901 atgtggcacc tacacgttca cccccagtga tcgaccggat tcgtagaagc cacgttcgag
    36961 gggctggatc ggccagggtg ggggcgggat gtgctcgttc tcttgcatgg tggggcatgg
    37021 tggacgatga taaatgcaag ctttcattac gtctggctgg cagtcgcgac cgcgtcatgt
    37081 ccacagggtt cgcggggcga tggcccagtt gatccgcact tacgtgtcgc cggttgaatc
    37141 cggcggcgaa cacacaggag ttgcgcaatg ggttcttcag agtttgatcc aaaatcagtg
    37201 ttgcgccggg cggccgatcg tcgttcggta ttgaagtatg gtgccgcctc cgctggtgcc
    37261 ttcggcgcct cccgctggct gggcgccagc gcgcaggacg cgtcaccggc ggcctcaccg
    37321 gttgatggcc ctgctgattt cggtaccggg tcgctggagc tcagcggcga cgtgacgatc
    37381 cagtactggc agtacgagct ggccgccaag acccagctgg tcgaagagtt gatcccggag
    37441 ttcgaggaag cgaacccggg catcacggtc gagcatgtga acttcccgta cgacgatttc
    37501 cgccagcagg tcgccgccgc cgtccaagcg ggcgaggggc cggacgtgct caacgtctac
    37561 tacggctgga tccccgccta cgtgcagcag cagttcctcg tgccgctgcc gcaggacatc
    37621 ttcccggccg aggtaatccg ggatgcgtat ttccccaccg ttcaggtggc cgaggtggag
    37681 ggagagtact acgcgctccc gacggcggtg cgcacgctgg cgctcttcta taacaaggac
    37741 atcctcgagg ccgccggtgt ggagccgccg agcaactggg aagagctggt cgaggttgcc
    37801 aaggcaacgg tccagaagga aggcgacaac ttcgaggtgg tcggcttcac ctgggatatc
    37861 ggcggccagg gccacaactg gtggcgcgag gccctcaacc ggcagaacgg ccagatcccc
    37921 tataccgatg accggcgtga gctgctctgg acagagccgg agtgcatcga ggcgttcgag
    37981 tacgcggtct ccttcctgac ggagcacggc gtgacgcagg ccggtttcca gacggacggc
    38041 gccacggcct tcgcggccgg caaggcggcg ctccacatcg acggctcgta tcgcctcggg
    38101 tccctggcca tcgatgcgcc ggacctcaac tacggcgtcg tgccgctgcc ggagcacaag
    38161 gagaaggcca gctttgcctc cttctgggcc aatggcatca cccgcaacgc ggcggagggc
    38221 gacaagctta tcgcctcggc gaagtggatc gacttcctct cctcggccga ggttcagcgc
    38281 cgctggacac cggcgatcgg tgagttgccg gcgcgtcagg cgcttgccag cgacccggcc
    38341 ctgctggaga acgagaagct ggtggcgttc atcgagtcgc tgccgtactc ctacgcgacc
    38401 ttcttcgtca acgaatccac cctgcggcag gcagtcatcg acgccttcga tcaggttgtg
    38461 ctgaacggcg tggacgccga gacggcggtc gcggaggcag aggcacaggt gcaagaggag
    38521 cttgacgagt actttgcaca gctcgactag tcaccgaggg tgaccgtggc gcgtcccgtt
    38581 cgggggcgcg ccattgcctg tactccctgg acggcaatcc atgagtttga gtctgcagcg
    38641 acgacgatgg ctttggggct acgcgttcat cgcgatcccg ctcttcttct tcctctttgt
    38701 ccgcatcggg ccgaccatct acgccttctg gatgagcctt catgagtggg atcccatcgc
    38761 tctcgatcgt ccctatatcg ggctcgagaa cttccggacc atggcggacg acgagacctt
    38821 ctggaaatcg ttgcgcaaca cctggatcta tgttggtgtc ggggttccgg ccgggttgat
    38881 catcagcctg ctgatcgcgc tggggctgca gcggatgatc cggtataccg gtttcttccg
    38941 ggtgctctat ttcctgccct acatcacgtc gctggttgcc gtgggctggg tatggcgctg
    39001 gatgtacatg cccaacggcc tgttcaacga cattctgggc tgggtggggc ttgggccata
    39061 caagtggctc ggtagccccg atatggcgat ctactcgatc atcgcgacaa cggtctggca
    39121 cggccttggt tttcaggtcg tgatctttct cgcggggctg gagagcatcc ccgaagacta
    39181 ccttgccgcg gcctcggttg acggcgcctc cgggtggcag aagttccggg atattgtggt
    39241 gccccagatc aacccgaccc tggtcttcct gatcgtgaca ggtgtgatca gcaacctgca
    39301 ggtctttacg cagatccggg cgatgagcag cgggggcacg ggcgggccgc tcgacagcac
    39361 catcagtatg gtgctctacg tctaccagga ggcgttccag tcgttgccct cccacatggg
    39421 ctatgcctcc gccatgacgg tcgtgctctt cctgatgatc ctggcgatca cgatcttcca
    39481 gctcaaggtg ctacagcgag ataccgaatg acgacaaaga cctacttccc ggatgaaccc
    39541 ggcgttcccc tggcaggggt aacgaatgac gacgcagcag cttcgagacg gactgcgatg
    39601 gctcgtgtgc gacgggacca gcggcgtcgc cgcgtggccc ggattgcgct gttgctgatc
    39661 ctcctgctcg gcgggctggc gatgatcttc ccctttgtct ggatggtggt gtcgtcgctc
    39721 aagaaggcgc aggacatcta cacgctctcg ctgatcccgc cagacccgac gctggggaac
    39781 tacacggagg tgttgcagcg caccagctac atcaggtggt tcctgaacag catcatcgtg
    39841 gcgggaatca cgacgctgag cgtcgccttc ttcgactcgc tggccgggta caccctggcg
    39901 aagttcaggt ttcccgggtc gaccgccatc ttcctgctca tcctgagcac gctgatggtg
    39961 ccgaccgaga tgctggtgat tccctggtac atgatgtcga tcgagttcaa ctggacggac
    40021 tcctactggg gcatcatgtt cccgggcgtt atcagcgcgt tcggtgtctt cctgatgcgc
    40081 cagttcttca tgggcctgcc caacgagctg atcgacgccg cccggctcga cggcttctcc
    40141 gagttccgga tcttctggaa gatcgggttg cctctcgtga agccggctgt cgcggcgctc
    40201 tgtatcttca cgtttctcgg gaactggaac gcctatatct ggccgctgat cgtgatcagg
    40261 tccgatacga tgcggacgct gccggttggg atcgcgttct tttcgagcga ggcgcagagc
    40321 tcattccacc tgatcatggc ggcggcatcg ctggcgaccg tgccggtaat catcatcttc
    40381 ctgatcttcc agcggcatat catcaagggc attgccctgg ccgggttgaa ggggtaggga
    40441 ggacggttgc tgcccagcag ggcggacatg gagtccgccc gtacaggtcc gtgtcggctc
    40501 ctggttatga atgatcacga ctgtccttga aggagaacct atgtccaacg acacggcggg
    40561 cacgtttccg catcccgcgt atgcccaaaa cgtgctggag ccggcctttg aggagagtaa
    40621 ggagcatctc tttgcgcaca tgatcgcagc caatgaggcg catgcgatca tgctggcgga
    40681 gtgcggcgtg ctcgatccgg cccaggcgtc ggcgctgctt accgccttgg gtgcggtgaa
    40741 gcaacagggt gctgaggcgt ttcgctactc gcccgagatc gaggatctct tcttcgcggt
    40801 ggagggtcgg ctgatcgagc tggtgggacc cgatgcgggg ggcaatctgc agattgcccg
    40861 gtcgcggaac gatctcgccg ccgcgatgag caggatgatg ctgcgtgagc gcctggttgg
    40921 cgcacggcag caggtgctcc atctgcggtc cgtgctgctg gacatgatcg agcagcatat
    40981 cgagacgctg atgccgggca tcacgcatac acagcccgcg caacccacaa cgctcgcgca
    41041 ctacctgctt ggggtgctcg gtccgctcga gcgcgacagc gatcggctct cgtgcgcctg
    41101 gaggcggacg aaccggagct cgctcggcgt tgctgcattt acgacgacca gtttttctat
    41161 tggccgtgag ttaacggcgg atctgctggg gtttgacggc tatgtcgaga atgggtacga
    41221 cgcagtaggg gcgaacgatc atctcctgga gtcggtccaa gcgctggtca actgtgtcgg
    41281 ttcgctctcc cgcttcgtct acgacctact ggtctgggcg cggaaggacg tcgatatcat
    41341 ccggatcgac gatgccttca tccagatgag ctcgatcatg ccgcagaagc gcaacccggt
    41401 ggtgctggag catgtgcgcg tccggatcgg gtgggtctat ggcgaggcgt cggccgtgga
    41461 aacgattatc cactcggcgg cgttcggcga caccaacgat gttgaggacc cgatgttcgt
    41521 cccgctgaac cgggcgttcg acgccgcgga gatggtcctc cggctcctgg ccgccgtgct
    41581 gtcgacggcg gcgttcaacg tatccggcat ggagcagcgt gcggcggagg gcaatacgac
    41641 gacgacggcg ctggcggatg cgctggtgca ggagcacggt atcccgttcc ggggagccca
    41701 caccatcgtc tcccggatcg tgagccgtac gatgagcgac gatgtgccga tcactgccga
    41761 acttgtgaac gaggtcagca gcgaagtgct gggcagatcg ctggatgtga cgcaggaatt
    41821 cgtcgcgaat gcgcttgatc cgtgggcctt cgtcaacgcg cgcacgatcc ctggtggctc
    41881 ggctccggag acaacccggc aggcgctgga gtcggcgcga ttgcacctgg acgaggatcg
    41941 gggagtcctc gaagccgaca atgccgccgt caaccatgcg agcgccgagc ggcagcgccg
    42001 gatagatgcc ttgatggctg gggccagttc gtagagtggc gtgagatgcc ggctcgcagg
    42061 gcaagccggg ggagcaggca ggctgccacg agggcggccc ctgccgtaca tcgcggtcag
    42121 ttcgtcccgg tcttctttag cgctctggac gacggtgttc cttctcactg agatcgagaa
    42181 agagagtcac ccgggcgtgt cctcgacatt gagggccgca tcgcggatgg cggtcaggag
    42241 gggatggtcg atctctttct gcgccatgtc ggtgaacccc tggaccggga tatcgggatg
    42301 cccgcgcagg taggtgcggg attggagaaa cgcctccagc cggtcgactt ccttgacgaa
    42361 gcgtgcctcg ggcgaactct gctcttcata ctctgaccag agcgacctca tctcctcgcc
    42421 aatgctctcc ggcatgagcg cgatcagatg cgcggctgcc cgctcctcgt cctggcgctt
    42481 ggcggccctg ttctccggtg atctcaggtg gcggacgctg aagaatgccc gcagtgcgtc
    42541 caggtcgccg ggtgggggaa tctcgtctgc ctcgtatggt gggcgatctc ccaccagcgc
    42601 ttccgccatg tcatgaatga tcgcgagctt caacacccga tcggcatcca gcgtcccgtc
    42661 ctcatgggcg aggaaccacg cgatcagcgc ggtcatcatc gtatggtccg cgaccgactc
    42721 gacctgttcc cgggggacgc ccctgtcgat ccagcccgtg cgtttggtgc gcttcaggtg
    42781 ctccagcgtg cggaaga
//
LOCUS       DASYWM010000237         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_217500, whole genome shotgun sequence.
ACCESSION   DASYWM010000237 DASYWM010000000
VERSION     DASYWM010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217500"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VGR29_09690"
     CDS             <1..795
                     /locus_tag="VGR29_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEV2073900.1"
                     /translation="VKNTIAVASGKGGVGKSTVAVNLAVALAQAGAKVGLLDADVYGP
                     STPLMMGVNERPLMRDNKIVPLEAHGVRVMSIGFILDPAKALIWRGPLVAQLINQFLN
                     DVHWEDLDYLVLDLPPGTGDVQLTLVQKIPISGAVIVTTPQEVALADAVKGLKMFQEV
                     KTPILGIVENMSWFTSPESGTKYPIFGEGGGQRTAEANNVELLAQIPIEMGIREGGDA
                     GEPLTVTHPQSETGQAFAHLAERVATQLAAQAVAKPRKPTIMLKTV"
     gene            complement(2393..>2575)
                     /locus_tag="VGR29_09695"
     CDS             complement(2393..>2575)
                     /locus_tag="VGR29_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073901.1"
                     /translation="VYRIAMERRGYALGPTAFARSYTIAARYTGLRYLWWTLTAWLED
                     QMMDEWVMHYVNMILR"
BASE COUNT          506 a          705 c          810 g          554 t
ORIGIN      
        1 gtgaagaaca cgattgccgt tgcttccggc aagggcgggg ttggcaagtc gaccgtcgcc
       61 gtcaacctcg cggtggcact tgcacaggca ggtgcgaagg tcggcttgct ggatgctgat
      121 gtttacggtc caagcacgcc actcatgatg ggcgtcaacg agcgaccgct gatgcgtgac
      181 aacaagattg tgcccctgga agcgcatggt gttcgggtga tgagcattgg cttcattctg
      241 gatccggcca aggcgctcat ctggcgtggc ccgctggtag cgcagctcat caaccaattc
      301 ctgaatgatg tgcactggga ggatcttgat tacctggtgc tcgatctgcc gccgggaacg
      361 ggtgatgtgc agctgacgct cgtccagaag atcccgatct ccggcgcggt tatcgtcacg
      421 acgccgcagg aagttgcgct ggccgatgcc gtgaaggggc tgaagatgtt ccaggaggtg
      481 aagacgccga tcctgggcat cgtcgagaac atgagctggt tcaccagccc cgagagcggc
      541 acgaaatatc ccatcttcgg tgaaggtggt ggccaacgca cggcagaggc aaacaacgtc
      601 gaactgttgg cgcagattcc gatcgagatg ggaatccgcg agggaggcga tgccggcgaa
      661 ccgctgaccg tcacgcatcc gcagtccgag accggtcagg catttgcaca tctggcggaa
      721 cgggtcgcga cgcagcttgc ggcgcaagcg gttgccaaac cacgcaagcc aacaatcatg
      781 ctgaagacgg tatagctgag accatcgcaa ggtgtatatc tggaaacccc ggtccctatg
      841 accggggttt ttcttgccaa actgcacgct accggcgcct ggagttgagc tcgctcaact
      901 cagcgggcgg atattcttcg gacgtctccc gatgtccgaa ggtatatccg tcgtgcgtcg
      961 atggtcttag tacgcgccgg agattccaat gcgcttcgtg caggattctc aggggtgagc
     1021 ccgtcagcga gcctttgctg cgctcagaat gacggggttt ggtcacggcg gacagtcacg
     1081 ccgtttggga accatctgcg acgaggggtt ccttgggaag ccaagaatgt tgggaccgcc
     1141 ccgggtggtc attcccgggc tgtctgcaag atatcgatct caatgcacgt tgtctacagt
     1201 ggagaagtta gggtgcaatg caatgagccg gtctggataa ttcaatcctc cggctcatgc
     1261 acatgattct ctttggctgt ctgtcgcgtg cagggcgttc acgtcacctt gccgcgattt
     1321 gccaccggag ccgttgttga cgacgcggtg gcgccaaggc ctgtcgagag cgtcgatacg
     1381 caaactctcc ctgctcctgg ttgtactgca tccacctggg ccgacaggtg tttcatgcct
     1441 atcgatcgga aagggtcgtt gcgggtcggc ggcatcatga aagttgatgg actatcgtgc
     1501 gcctgttgac ctctcctgaa ctcctctcgt agatgttcca cccatgagca tgaaggcgga
     1561 ttccgccagc aaactctcac cggactacga tgaagcgaac gatcaggaca gtcgacatgc
     1621 tccacaggaa cggcgtgtcg aggaccgtgt gttccgtggc tcgtcagtcc gatcttgcac
     1681 tcgagggtgc attccgtgtc gatgtcctct gacgccgacg ttgtttgcgg ctgacgcctg
     1741 gttcacgaga gatagcgata aggtggactc gcacatccat ccagcatcgg ctatcacggt
     1801 cgccaacatc gcggtggagc ttccgccgct gctggtcctt cgccgggcat ctggtaccgg
     1861 cgaatcaatg gaagcgacgc gtgcgctgga ttcatcgggt gaggatcggg agacttcgcg
     1921 accgcggagc cgcaggatac tcgccgtttg atccgcgcct gcggaccttc cggccggttc
     1981 tcgctttcgc atcaatcgga gtttcgaggt gatccgccgc gggtcgtctt gcggcctgcc
     2041 atgcgaagcc tcggtgactt ccgggtgttg cgaatacaga gatccggcaa ctatcgaatc
     2101 atcagtcaat cctggcctgg ttcgcgttgt atggagggtc atgtcccgcc tgtcgcgttg
     2161 gccggcgagt gatgtttcga tacctcgatc ctagcagaga gcaggggttt gtcaagaggt
     2221 caaagtgagg acgtgatcaa gtcgaaggac aaaacccggg agtaacggga aatgtgatga
     2281 cgtggatttc ctgggacagg tggtgtaata cgcgttcggt ttttatcaat tgctcagtgg
     2341 ggagcccgga ctgtcgcaca taccttcgtt tcgaacatgg cggggacagt ggtcagcgga
     2401 ggatcatgtt tacgtagtgc atcacccatt cgtccatcat ctgatcttct agccaggccg
     2461 tgagtgtcca ccagagatac cgcagtcccg tgtaacgcgc cgcgatggtg taagagcggg
     2521 cgaacgccgt agggccaagg gcatagccgc gtcgctccat ggcaatgcgg tacac
//
LOCUS       DASYWM010000238         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_461643, whole genome shotgun sequence.
ACCESSION   DASYWM010000238 DASYWM010000000
VERSION     DASYWM010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461643"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VGR29_09700"
     CDS             <1..1167
                     /locus_tag="VGR29_09700"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEV2073902.1"
                     /translation="ALEVAEGRWDDEFPIDLFQTGSGTSTNMNANEVISSRARQLMPD
                     ATPPHPNDHVNMGQSSNDVIPTTIHVAAYIAISEHLLPALEHLEQTIGERSKELRDVV
                     KTGRTHLMDATPVRLGQTVGAWETQVRLARERIENTLPHLARLAIGGTAVGSGLNAHV
                     EFGARVAANLSERTGHPFVETDDHFESQASQDTAAEVSGALKAYATGLIKIANDLRHM
                     NSGPIAGLGEIVLPALQPGSSIMPGKINPVIAEASMMVAAQVIGNDAAITVGAQHGNF
                     ELNVMLPMIAHNLLESIELLSTMSLVLADKAIAGFTVNTERITELVDRNPIMVTSLNP
                     IIGYDNAAKIAKQAYAEGRRVKEVAAELTDLSEAELDRLLDPMELADPGIKGGA"
     gene            complement(1300..2958)
                     /locus_tag="VGR29_09705"
     CDS             complement(1300..2958)
                     /locus_tag="VGR29_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2073903.1"
                     /translation="MIPVEQETQAAPASTTSRVPPEELPGSFIRRRVPYLFSIRFGLT
                     AWYAGVLILTLAISGVALRAMLVRSLEQDAEARLIEAARDIRGMTSETGLITDPRGDT
                     GEMSLILLPDFDAESILATGLSITIIDTSASGPLFEKGPLSNRWPQPQDYRAYLKLRE
                     PTYFIHEISDQSFRGLAYPILSRQAPDPETGQRSVIGVIFTSESIESDNRVVARLNQV
                     LLATGLAGVVVATFGGWMLAGRALAPVNRIITSADDIAKGKGEVSLSRRLDVPQTGDE
                     IAQLAETFNDMLDRIEEAFAAQHRFVGDASHELRTPLTAIKGNIDVLRRQIASGRGMD
                     PADTSEALADVSRETDRMGRLVDDLLSLARTDAEGFGSIVNLDAVSLDTLAREAIRTA
                     EVLVNGQELVLNAPVPVMIHGDGDRLVQVMLILLDNALRHTPPGGRVTLSISTAIDPH
                     DHVTCACIDVEDTGKGIPAEHLPHLFERFYRAEGSRSRLDGGTGLGLSIALSIVRSHQ
                     GWIDVESAPGHGTHVTVWLPMLDEDTDTDGPASRRLPRIPRRRS"
     gene            complement(2955..3704)
                     /locus_tag="VGR29_09710"
     CDS             complement(2955..3704)
                     /locus_tag="VGR29_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2073904.1"
                     /translation="MVTPFPSAEPESSDAPKRILLVEDEPAISGFVRRGLIFEGYDVT
                     VVTDGRDALEAIRDRPPDILILDLMLPNLDGINVTQRIRAAENADAGERLPILMLTAR
                     DSVADRVAGLNAGADDYLVKPFDFEELVARVRALLRRTRPPAAAQANEILQFADITLD
                     LTSRTLTRGEEEIELTPREFDLLALFLRHPNQVLTRSTLMQRVWGEDFYGESNVLEVV
                     IGNLRRALEISDHPRVIQTVRGVGYVLRQRA"
BASE COUNT          782 a         1143 c         1293 g          697 t
ORIGIN      
        1 gcgttggagg tcgccgaggg caggtgggac gatgagttcc cgattgatct cttccagacc
       61 ggctccggta cctcgaccaa tatgaatgcg aatgaggtca tctcgtcccg cgcaaggcag
      121 ttgatgccgg atgcgacgcc accgcacccc aacgatcacg tcaatatggg ccagagcagc
      181 aacgatgtga ttccgacaac gatccacgtt gccgcctata tcgcgatcag tgagcatctg
      241 ctgccggcac tcgaacacct cgagcagacc atcggcgagc gctcgaagga gttgcgcgat
      301 gtcgtgaaga ccggtcgtac ccacctgatg gatgccacac cggtgcgcct cggtcagacg
      361 gtcggcgcat gggagacgca ggtgcggctg gcccgcgagc gaatcgagaa cacgttgccg
      421 cacctggcac gtctcgccat cggcggcacg gcggtcggca gcggcttgaa tgcccatgtc
      481 gagtttggcg cgcgcgtggc ggcgaatctc tccgaacgga cgggacaccc gttcgtggag
      541 acagacgatc actttgagtc gcaggccagc caggatacgg cagctgaggt gagtggcgca
      601 ctgaaggcct acgcgaccgg gctgatcaag atcgccaacg acctccggca catgaacagc
      661 gggccgatcg cgggcctcgg ggagatagtg ctgcccgcgc tgcagccggg ttcctccatc
      721 atgcccggca agatcaaccc ggtcattgcc gaggcgtcga tgatggtcgc tgcccaggtt
      781 atcgggaatg acgcggcgat cacggtgggc gcgcagcacg gcaacttcga gctaaatgtg
      841 atgctgccga tgattgcgca caacctgctg gaatccatcg agttgctctc gacgatgtcc
      901 ttggtgctgg cagacaaggc gatcgccggg tttaccgtga acaccgagcg gatcacggag
      961 ctggtcgaca ggaacccgat catggtgaca tcgctcaacc caatcattgg ctatgacaac
     1021 gcagcgaaga tcgccaagca ggcgtatgcc gaggggcgac gtgtcaagga ggttgccgcc
     1081 gagctgacgg atctcagcga agctgagctg gacagattgc ttgatcccat ggaactggcc
     1141 gatcctggta tcaaaggtgg cgcatagcgc ggaacgaggc ggccctgacg acggcgtgcg
     1201 ggcgaaccaa aggcatttcg cggcaatcga gcctgtacgg cccttttggg ttcgccttcg
     1261 cttcaaccgg gcgaacatag agtccgcccc tacgccaatc taggagcgcc ggcgtgggat
     1321 gcggggcagc cggcggctgg ctggcccgtc agtgtcggta tcttcgtcga gcatggggag
     1381 ccagacagtg acgtgagtcc cgtgaccggg agcgctctca acgtcgatcc acccctggtg
     1441 cgagcggacg attgacagcg cgatcgagag gccgagtccc gtgccgccgt cgagccggct
     1501 acgggagcct tcggcgcggt agaagcgctc gaagagatgg gggagatgtt ccgccgggat
     1561 gcccttgccg gtgtcttcaa catcgatgca ggcacacgtg acgtgatcat gcgggtcgat
     1621 cgccgtcgag atcgagagcg ttacccttcc accgggaggc gtatgccgaa gggcgttgtc
     1681 gagcagaatc agcatgacct ggaccaatcg gtccccgtct ccatgaatca ttaccggcac
     1741 cggcgcgttg agaaccagct cctggccatt gaccagtact tcggcggtgc gaatggcttc
     1801 cctggcgagg gtgtcaaggg acacagcatc gagattcaca atcgagccga acccctcagc
     1861 gtcggtgcgg gcgagggaga ggagatcatc taccaggcgg cccatcctgt ctgtctcccg
     1921 gctgacatcg gcaagggcct ccgaggtatc ggcggggtcc attccacgtc cggatgctat
     1981 ctgccggcgg aggacgtcga tgttcccctt gatcgctgtc aacggtgttc gtagctcgtg
     2041 ggaggcgtct cccacaaagc gatgctgggc cgcgaaggct tcctcgatcc ggtcaagcat
     2101 gtcgttgaag gtttcggcaa gctgggcaat ctcgtcgccg gtctggggaa cgtcgaggcg
     2161 ccgggacaat gagacctcgc ccttgccttt cgcgatgtca tcagcagacg tgatgatgcg
     2221 gttgacgggg gcaagggccc gcccggccag catccagcca ccgaatgtcg caacgacgac
     2281 tccggcgaga ccggtcgcca gcaagacctg gttcagccgg gcgacaacgc ggttatcgga
     2341 ttcaatggac tcacttgtaa atatcactcc aatcactgac cgctgccctg tttcaggatc
     2401 cggcgcctgt cggctcagga tcgggtaggc aagcccgcga aaggattggt ccgatatctc
     2461 atggatgaaa taggtcggct cccggagctt taggtaggcc cggtagtcct gcggctgtgg
     2521 ccaacggttc gagagtgggc ccttttcgaa gagagggccg ctggcgcttg tatcaatgat
     2581 ggtgatcgaa agccctgtgg cgaggattga ctcggcgtcg aagtccggca gcaggatgag
     2641 agacatctcg cctgtgtctc cccgcggatc ggtgatgaga cccgtctccg acgtcattcc
     2701 ccggatgtcc cgcgcggcct cgatgagccg ggcctcggca tcctgctcga gggagcgaac
     2761 gagcatcgcg cgcagcgcga cgccggagat cgcgagtgtg aggatgagca ccccagcata
     2821 ccaggcggtg aggccgaagc ggatcgagaa gagataggga acccggcgcc gaatgaacga
     2881 tcccggcagc tcttccgggg gaacccggga agttgtgctg gcgggagcgg cctgcgtttc
     2941 ctgctctacc gggatcatgc gcgctgccgg aggacataac ctacgccgcg aacggtctgg
     3001 atcacccttg gatgatcgct gatttccagg gcacggcgaa gattcccaat gacgacctca
     3061 agcacattgg actcgccgta gaaatcctcg ccccagacgc gctgcatcag ggtcgaccga
     3121 gtcagcacct gattggggtg acgcaggaag agcgcaagga gatcgaactc ccgaggggtg
     3181 agctctatct cttcttcgcc acgagtgagt gtccggctgg tgagatcgag cgtgatatcg
     3241 gcgaactgca ggatctcgtt cgcctgggcc gcggcaggtg ggcgcgtgcg cctcagcagt
     3301 gcacgcacgc gggcgacgag ctcctcaaaa tcgaacggtt tgaccaggta gtcgtcagcg
     3361 ccagcattca ggccggcaac gcggtcggca acggaatcgc gtgctgtaag catcagaata
     3421 gggagccgct cacctgcatc agcgttctcc gcggcgcgaa tgcgctgcgt gacgttgatg
     3481 ccatcgaggt taggcagcat gaggtcgagg atgagaatat cgggaggtcg atcgcggatg
     3541 gcttcaaggg cgtcacgccc atcggtcacg acggtgacgt cgtagccttc aaagatcagc
     3601 ccgcgacgta cgaacccgga aatcgccggc tcgtcctcca ccagcaggat gcgcttcggc
     3661 gcgtcactgg attccggctc tgctgacgga aacggcgtaa ccatgaggtt cctggtccct
     3721 tggaatcggc gagtaatcca gcgagcgagg aagtctcgca tggcgcacga tatgacatgt
     3781 atcttaagca tatcgcaccg tagacgcctc ctgagaacgc aggaacggcg ggtcattggg
     3841 gaatgacagc cgccgttcac gcgtggggtg gggaggagag aatcgaaaag ctcttcgcgt
     3901 atgtttgtaa tcagg
//
LOCUS       DASYWM010000239         9234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_648939, whole genome shotgun sequence.
ACCESSION   DASYWM010000239 DASYWM010000000
VERSION     DASYWM010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9234
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648939"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..697)
                     /locus_tag="VGR29_09715"
     CDS             complement(155..697)
                     /locus_tag="VGR29_09715"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEV2073905.1"
                     /translation="MSLATPRELLIHELSDTMSAEHIVHKVLGQMASETENTEARKAM
                     EHHQKETEQQIKNLEKVFNMLGEQPEQTTCHAAEGLKKEHDALTEEKPQGHVKELGLL
                     AGAGKTEHYEIASYTMLHRMAKDLGEKEVAELLKENLDQEKEMARTVESLAKEVGKEM
                     KVEMKEQAKQEKEAEKAAAS"
     gene            1386..2069
                     /locus_tag="VGR29_09720"
     CDS             1386..2069
                     /locus_tag="VGR29_09720"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV2073906.1"
                     /translation="MVFRRDNKVDQFQRQMSALRHQIGGEAGSEQDEMDKDVPDIADD
                     RFPNTRFNDSEQYDRQDAGGYSFGSYPAAPQDQDEDDLIDGALAVPDMPGVDSQISVV
                     AQGALWKGDVEADGSIHVFGRVEGTLTAKEDIWLAEGSDVHAVVTARQVIVAGQVNGT
                     VRALGRFEALPQGVVEADVQAPSFVVHEGATINGKLTMSSNDNTVSSGRAERAGSTPI
                     VQRRMRPGS"
     gene            2128..2745
                     /locus_tag="VGR29_09725"
     CDS             2128..2745
                     /locus_tag="VGR29_09725"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV2073907.1"
                     /translation="MSSRAADQDGFRGYVPQTTAENQESISVIDQYSNFDGTYNSTRD
                     LRIEGQVKGTIECRGTLHIAQGANVNASVEAENISVAGELDGEITCRGKLLLLPSGRV
                     RGKINTRTLIIHEGAFYEGELEMNGESGRPTGRSARNRSQAPVGQGSSSALGPREPAS
                     DEDAEPQPAAQTGSPSTFIRRFGGQEQQWDGARAGDNETRENVDT"
     gene            3186..4013
                     /locus_tag="VGR29_09730"
     CDS             3186..4013
                     /locus_tag="VGR29_09730"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2073908.1"
                     /translation="MKLPKFSSPLKLFSPAPPRPALGLAGIGGNMMFASGGGGGDDYD
                     PDDFDEYDDEPVEPERRPRASRTSRASGDTRDTVVGRPRTAQYQPEERYWTDYLRIAL
                     PVIGLLLMIGLLWFWADRLINDDPDVTEPTSREEIGLVTTAAETDPPAQIISTPPPAD
                     TTAPGGVTEMTPAAGADTSGETGDTQAADETDPPEPPADTGGNTYTDGDTVTTTESIN
                     LRPDPSTSGDPIAVLEAGDTLTINGGPEEGETYVWWEVVTEDGTTGWVVEDFIQPAQ"
     gene            4283..5098
                     /gene="rpsB"
                     /locus_tag="VGR29_09735"
     CDS             4283..5098
                     /gene="rpsB"
                     /locus_tag="VGR29_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV2073909.1"
                     /translation="MATTDTTRRNPRVSLKTLLEAGVHFGHQKNRWNPKMKPYIFTER
                     NGIHILDLQQTVPMLERAHETVSEITSKGGHILFVGTKKQATEIVKHEAERSGQFYIN
                     RRWLGGTLTNFVTIKNRLRVLKNLQSEKERGEWDYLPKQEAARKELQLDKLERTLGGM
                     RDMTQLPGAIFIIDPKREHLAVHEATRLGIPTIAMVDSNSDPTPIIYPLPANDDAIRS
                     LRLITAGIANAAIAGRTERESTHVEDEGFSDAVAAQDAAAGAAEKERDSGGDE"
     gene            5149..5763
                     /gene="tsf"
                     /locus_tag="VGR29_09740"
     CDS             5149..5763
                     /gene="tsf"
                     /locus_tag="VGR29_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEV2073910.1"
                     /translation="MENNNFHCTQQLEGAPMAITTDMIKTLRTKTGAGIMDSKRALEE
                     TDGNIDKAVDFLRQQGLAKAGKKADRAARQGLIDTYIHAQGRIGAMVEVNCETDFVAR
                     TEDFKRLAYDISMQVAAMAPRYATVNEIKEEDYAALEREFGDRDAAIKAVVLSEQTFI
                     KDAKKTISDMVKENISKLGENIVIRRFARFELGETSSDESTGDA"
     gene            5862..6596
                     /gene="pyrH"
                     /locus_tag="VGR29_09745"
     CDS             5862..6596
                     /gene="pyrH"
                     /locus_tag="VGR29_09745"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV2073911.1"
                     /translation="MKDAAKTRRVLLKVSGEALMGEAPFGIDPSIAVRMAQEVQEAHL
                     QGIQVGIVVGGGNIWRGLSASASGMDRATADYMGMVATVLNGLALQDALENEGVQSRV
                     MTAIAMHEVAEPYIRRRAIRHMEKGRVVILVAGTGNPYFTTDTAAALRALELDVDMVL
                     MAKNKVDGVYSADPHTNPDATRYDHITHQEALERNLKVMDASAMALCRDNDLPILVFD
                     VSTRRAITRALLGDSIGTLVSSTSSQ"
     gene            6661..7221
                     /gene="frr"
                     /locus_tag="VGR29_09750"
     CDS             6661..7221
                     /gene="frr"
                     /locus_tag="VGR29_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV2073912.1"
                     /translation="MTVADVIKDTETRMGKAMDSLHHHLNSVRTGRAAPSLVEDVKVD
                     YYGASMPLNQLASISAPESRLLVIQPWDKGSMGPIEKAIQTSGLGLTPNNDGQLIRIS
                     IPALTEERRKQMVKMVGGHVEDAKVAVRHIRRDAIQSVKTLLNNKEIGEDDERRGITE
                     LESVTKKYVEQADSIGKAKEQEVLEV"
     gene            7223..8002
                     /gene="uppS"
                     /locus_tag="VGR29_09755"
     CDS             7223..8002
                     /gene="uppS"
                     /locus_tag="VGR29_09755"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEV2073913.1"
                     /translation="MAEQASGHLPVATHASPEDHPGIANDRVPEHVAIIMDGNGRWAS
                     RRGLSRIEGHEAGTENIRRITHRAGQLGIKYLTLWAFSTENWRRPKEEVEGLMRILGN
                     VIESETQELHQQGARLRHIGDLDALQPDLRQSVLDAMELTRNNDKLVLTLAFNYGGRQ
                     EILHAVKKMIEDGVAPEDVSEEAFAERLYMPGLPDADLIIRTSGEWRLSNFLLWQGAY
                     AELFFTPKLWPDFQPDDLTEAVVDYSQRERRFGAIAPPSNG"
     gene            8007..8861
                     /locus_tag="VGR29_09760"
     CDS             8007..8861
                     /locus_tag="VGR29_09760"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV2073914.1"
                     /translation="MRERSISALGIVILGLVPALVGGVIFAFAFTVVTVIAYRELLPL
                     LHIRQRRLEVVGYLLIGLAGFLAWLYPDGRYLPLVVACCMLVPLTSILHPRSDTRDTG
                     DWATCTGAVLYLMLPTFAAIALRGTEGSVDRQWFQSLADAMPSSGATAEGLGLFLTAL
                     LITWLSDTAAYLVGKSVGRTKLIPRVSPNKTVEGAIGGLIAAGTTSVVCVVGFGLDVH
                     PLTALIVGILIGATGMVGDLSESMLKRRAGVKDSGNLIPGHGGMLDRIDALIFVVTVT
                     WALLPALR"
     gene            8895..>9234
                     /locus_tag="VGR29_09765"
     CDS             8895..>9234
                     /locus_tag="VGR29_09765"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEV2073915.1"
                     /translation="MTGIALLGSTGSIGEQTLDVIATMEDRFRVVTMAAGLRHDLLSR
                     QVARFHPDMVVSAHESPDGNRPSPQQLIEAATHPDVDIVVVATSGHDAIPAVIAALEA
                     GKAIALANKEA"
BASE COUNT         1977 a         2731 c         2695 g         1831 t
ORIGIN      
        1 gaaatgaagg tcgagatgaa ggagcaggcc aagcaggaga aagaggccga gaaggccgct
       61 gcatcctgac tttaacagct gtcagatgat caacaaggcg gggcggcact ttcggtgtcg
      121 ccccgccttg ttgatcatat gaccgctgtt aaggtcagga tgcagcggcc ttctcggcct
      181 ctttctcctg cttggcctgc tccttcatct cgaccttcat ttccttgccg acctctttgg
      241 caagcgactc aactgtccgc gccatctctt tttcctggtc gaggttttcc ttcaacagtt
      301 cggcaacctc tttttcaccg aggtccttcg ccatccggtg gagcattgtg taactggcaa
      361 tttcgtagtg ttcggtcttg ccagcgccgg ccaggaggcc caactccttg acatgaccct
      421 gaggcttttc ttccgtcagg gcatcatgtt ctttcttcag accctcggca gcatggcagg
      481 tggtctgctc gggctgctct cccaacatat tgaaaacctt ctccagattc ttgatctgtt
      541 gctcggtctc cttctgatgg tgctccatag cctttcgcgc ttcggtattc tcggtttcac
      601 tggccatctg accaagtacc ttgtgcacga tgtgctcggc gctcatcgta tccgacagct
      661 catggatcaa cagctctcgt ggagtcgcaa gtgacattgt gatcgctcct cgtcacgtcc
      721 cgaacatctg gctccggggc cgtgcgctac aaaaagtatg ggtcttctcg ccggacattt
      781 ccggcgctga attggtctat tgcattcgcc atgccaagcg tccgtacacc cattatcggc
      841 agacgacgta gcgctgtgag acacgcctat tccgctatac accctttcgt gcaggcctgc
      901 cgggattcca gcatccacgt cggatcagca agaaagggtc tcgcgcatac gacctaaaag
      961 gaggaaatat cgtttttgcg ccacgccggc atacaactgg agcaatctgg tgtgagacaa
     1021 gtaccagcat ctaatgtggt ccccatgttg tcgtcaacta cacggttcgt cagtgttgta
     1081 cgtgcgttcc gtccatggct ttcctccaca ctctccccac tacccgtcac caccacctgg
     1141 ccatttggac aaacactgga caaacgcgcg tagtgtacat acacttgcag caatcgtgga
     1201 tagtagtacg caacacgccg tgtcgtactc gttgtcgcag gcttcttcct tcggcagttc
     1261 cgcttcccgc aaccgtgcgg gaatctgact acatgccgca ctggcccaac ggtcggcgct
     1321 cggtgcatgc gatcatcact ccgcgagcag tgcaaccgct tgttacgggg tgaggaggtg
     1381 gagacgtggt cttccgacgt gacaataaag tcgaccagtt ccaacgccag atgagcgccc
     1441 tccgccacca gatcggcggt gaagctggct cggagcagga tgagatggac aaggacgttc
     1501 ccgacattgc ggacgaccgg tttcccaaca cccggttcaa cgattcggaa caatatgaca
     1561 ggcaggacgc tggaggttac tccttcggat cttaccctgc cgcgccgcag gatcaggatg
     1621 aggacgactt gattgatggt gctctggcag tgcccgacat gccgggagtg gacagccaga
     1681 tcagtgtcgt tgctcagggt gcgctctgga aaggcgacgt cgaagccgat ggaagcatcc
     1741 atgtgttcgg ccgggtcgag ggcaccctaa ccgcaaagga agacatctgg ctggccgaag
     1801 gatcggatgt tcacgctgtt gtcactgccc gacaagttat cgtggccggg caagtcaatg
     1861 gcactgttcg ggcgctcggt cgcttcgagg ccctgcctca gggagtcgtc gaggcagacg
     1921 ttcaggcgcc aagctttgtc gtccatgagg gggccacgat taacggcaaa ctgaccatgt
     1981 cctcgaacga caatacggta agttctggac gagctgagcg ggcaggatct accccgatag
     2041 tacagcgccg gatgcggcca ggatcctagg tccgttagat cccgcacggt gggatcttcc
     2101 tggcccagtc cttcggagag gtgaacgatg tccagcagag cagccgatca ggatggtttc
     2161 cggggatatg tcccccagac cactgccgag aaccaggaat cgatcagcgt gatcgatcaa
     2221 tattccaatt tcgacggaac atacaattcg actcgtgacc ttcggatcga gggtcaggtc
     2281 aagggaacca tcgagtgccg aggaacgctg catattgcgc agggtgccaa cgtcaatgcc
     2341 agtgttgagg ccgaaaacat ctccgtcgcc ggggagctcg atggggagat tacctgtcgg
     2401 ggcaaactcc tgctgcttcc gagcgggagg gtccgtggca agatcaacac cagaactctc
     2461 attatccacg agggagcatt ctacgagggt gagcttgaga tgaacggtga gagcggccgt
     2521 cccacaggca ggtcggcccg gaaccgctcc caagctccgg ttggacaagg atcctcatcc
     2581 gcgttgggcc cacgtgaacc agccagcgat gaagatgcag aacctcagcc ggctgctcag
     2641 accggatccc cgagcacctt catccgccgg ttcggtggac aggaacagca gtgggacggt
     2701 gctcgcgccg gcgacaacga gactcgggaa aacgtcgaca cttgattttg ctcccaatcc
     2761 gttgtcacgc tggaggcggc gccgagatga cacaatattc ggcgctgccg cgtttaagcg
     2821 agtgtctgac gccgggacgg cggccttgcg gtactatgtg ttggagaggg atgcacctcg
     2881 tgaggacatc ccgggacgca tcgaaaagct tgaattactg gctcgaccag agttgaaatc
     2941 agttgtcgga aggattcaaa caccgcatgt cgggagcaac ggaccgtcgg tgtgggacat
     3001 gtcgacattt cgagccggcg ggctcatggc ggcgcggatg gtgccgaaat acgctgttgt
     3061 tcgcaccggg acagagttat caggtacaga gcgaggaact cgattgcagc cgcggttcag
     3121 gtgatttctg ggagccgccc agggattcat ccggagaatc gctagatgat gcaggtaagc
     3181 cgaacgtgaa actgcctaaa ttctcctccc cactgaagct cttttcacca gctcctcccc
     3241 gaccggcact tggattggcc ggtatcggcg gcaacatgat gtttgccagc ggtggcggtg
     3301 gaggcgatga ctatgacccg gatgatttcg acgagtatga cgatgagccg gtcgagccgg
     3361 agcgtcggcc ccgtgcctcc cgaacttcca gagcatccgg agacacgcgt gataccgtcg
     3421 tgggccgccc tcgaaccgcc caataccagc cagaagagag gtattggacc gattatctgc
     3481 gcatcgcctt gccggtgatc ggcctcctgc ttatgatcgg cctgctctgg ttctgggcag
     3541 atcgcttgat caatgacgat ccggatgtga cagagccaac ttcccgtgaa gagatcggac
     3601 ttgtgacaac tgctgctgaa acggatcctc ccgcgcagat catcagcacg cctccacccg
     3661 ccgacaccac tgcaccaggc ggggtgacgg agatgacgcc tgccgctgga gccgacactt
     3721 ccggagagac gggggacacc caggccgcgg acgaaaccga tcctccagag ccgccagcag
     3781 ataccggcgg caatacctac accgacggcg acacggtcac cacaacggag tcgattaact
     3841 tgcggcccga ccccagtacg tcaggggacc cgattgctgt ccttgaggcc ggcgacacct
     3901 tgaccatcaa tggtggacca gaagagggtg aaacgtacgt ctggtgggag gtcgtcactg
     3961 aggacgggac cacgggttgg gtcgtcgagg actttatcca gccggcgcag tgaattcccc
     4021 aaggattact ggatgaggaa cgaagggagg gatgctttac tgcatccctc ccttcgttcc
     4081 tgcgccacct acctgtccgg ggagtgacgc tacggtggtc ccggctagcc agggtgctat
     4141 actttggggt ggctcggcca acgctgagcc tattcaatga cacacgtgtc gtgaccgggt
     4201 gtggtgtgcc gaacggggag ttcctgtacg gtcctcactc gggttgaccg gcgcggaaga
     4261 ccaacaccca aaggaaagcc acatggcaac cacagacacc acgcgtcgta acccgcgtgt
     4321 cagcctcaaa acgctcctcg aagcaggcgt gcacttcggc caccagaaaa accgctggaa
     4381 cccgaagatg aaaccctaca tcttcaccga gcgcaacggt atccacattc tcgatcttca
     4441 gcagacggtt cctatgctgg agagagcgca tgaaaccgtt agcgagatta cctccaaggg
     4501 tgggcacatc ctctttgtcg gaacaaagaa gcaggccacg gagatcgtca agcacgaggc
     4561 ggagcgcagc ggccagttct acatcaaccg gcgctggctc ggtggaacac tcaccaactt
     4621 tgtcacgatc aagaaccggc ttcgcgttct gaagaacctg cagtccgaaa aggagcgtgg
     4681 agagtgggac tacctgccga agcaggaggc cgctcgcaag gaactccagc tggataagct
     4741 ggagcggact cttggcggta tgcgtgacat gacccagctt cccggggcta tcttcatcat
     4801 tgaccccaag cgcgagcatc ttgccgtaca tgaagctacc cgtctcggta tccccacgat
     4861 tgccatggtc gattccaaca gcgaccctac acccatcatc tacccgctgc cggccaatga
     4921 cgacgccatc cgctcgcttc gcctgatcac tgctggcatc gccaatgcgg ccatagcagg
     4981 ccgcacggaa cgtgagagca ctcacgtgga ggatgaaggc ttctccgacg cagtcgcagc
     5041 ccaggatgcc gctgctggtg ctgccgagaa ggagcgagac tccgggggcg acgagtaatt
     5101 ccatcgggca agtgtggcca ggaaatcggc ccggtccaca cctgcccgat ggaaaacaat
     5161 aatttccact gcacacaaca attggaagga gcacccatgg cgattaccac cgacatgatc
     5221 aagaccctgc gcaccaagac cggcgccggg atcatggata gcaaacgcgc gcttgaagag
     5281 accgacggca acatcgataa ggcggtcgac ttcctgcgcc agcagggcct tgcaaaggcg
     5341 ggcaagaaag ccgaccgcgc ggcccggcag ggcctcattg acacgtacat ccacgctcaa
     5401 ggacgtatcg gcgcgatggt tgaggtcaac tgcgagaccg acttcgtcgc ccgcactgaa
     5461 gatttcaagc ggctggcgta cgacatttcc atgcaggtcg ccgcgatggc gcctcgctat
     5521 gccactgtca atgagatcaa ggaagaggac tacgcggccc tcgagcgcga gttcggcgac
     5581 cgtgatgctg ctatcaaggc agtcgtcctc tcagagcaaa cgttcatcaa ggatgccaag
     5641 aagacaattt cggacatggt gaaggaaaac atttccaaac tgggggagaa catcgtcatc
     5701 cgccgttttg cccgctttga attgggtgag acgtcctcgg acgagagcac aggcgacgcg
     5761 taagtcgcac accgaaaggg aacgccgtct cctaccaaca ggggacggcg ttttttccga
     5821 gccgcgatca gggttgaccc acatcaggaa aggtgccgcc tgtgaaagat gccgcaaaga
     5881 ctcgacgagt cctgctgaag gtgagtggcg aagcgctcat gggagaagca ccctttggca
     5941 ttgaccccag catcgctgtc aggatggcac aggaggtcca agaagcccat ctccagggga
     6001 ttcaagtggg cattgtcgtg ggtggcggca acatctggcg cggcctttcc gcaagtgcat
     6061 ccggcatgga tcgggcaacg gcagattaca tgggtatggt cgcgacagtg cttaatgggc
     6121 ttgccttgca ggacgcactg gagaacgagg gcgtccagtc tcgcgtgatg accgctattg
     6181 cgatgcatga agtggccgag ccctatatcc gtcgccgggc catccgccac atggaaaagg
     6241 gccgtgtcgt cattcttgtc gctggcaccg ggaaccccta cttcaccacc gacactgccg
     6301 ccgcactacg cgcgctcgag ctcgatgtgg atatggtgct catggccaag aacaaggttg
     6361 acggcgttta ctcagctgac ccacatacca acccggacgc gacccgctac gaccacatca
     6421 cccatcagga agcactggaa cgcaatctga aggtgatgga tgcttcagcg atggcgcttt
     6481 gccgtgataa tgatctgccg atcctggtct tcgatgtatc aacgcgacgc gcaatcacac
     6541 gcgccctctt gggtgattct attgggactc tggtatcctc cacgtcatca cagtaagccg
     6601 acatcgagcg gctcgggtgc cgtctccggt gcgcagggac cccactgaaa ggacggaaga
     6661 atgaccgttg cggatgtcat caaggatacc gagacccgaa tgggcaaggc gatggactcg
     6721 ctccaccacc atctcaattc cgtacggacc ggtcgtgccg ctccctctct ggtggaggat
     6781 gtgaaggtcg actactacgg ggcttccatg ccactgaatc aactcgccag catttctgcc
     6841 ccagagagcc gcctgcttgt gatccagccg tgggataagg gcagtatggg accgattgag
     6901 aaagctatcc agacgtccgg gctcggcctg accccaaaca atgatggtca gttgatcagg
     6961 atctccattc ccgccctcac cgaggaacgc cgcaagcaga tggtcaagat ggtcggcggt
     7021 catgtggagg acgcgaaggt cgcggtccgg cacatccggc gcgatgccat acagtccgtc
     7081 aagaccctcc ttaataacaa ggagataggt gaggacgacg aacgacgggg gatcacggag
     7141 cttgagtctg tcacaaagaa atacgtcgag caggccgaca gcattggcaa ggccaaggag
     7201 caagaggtcc tcgaggtcta gcatggcgga gcaggcttcc ggacatctcc cagtcgcaac
     7261 tcacgcatca ccggaagatc acccggggat tgctaacgac cgtgtgcctg agcatgtggc
     7321 aatcattatg gacggtaacg gacgctgggc gtctcgccgc ggattgtcac gcatcgaggg
     7381 ccatgaggcg ggcaccgaga atatccgccg catcacgcat cgtgccggcc aactcggcat
     7441 caagtacctg acgctctggg cgttctcaac cgagaactgg cgacgcccga aggaagaggt
     7501 tgagggactg atgcggatac tcggcaacgt gatcgaatcc gagactcaag agctgcatca
     7561 acagggcgcc aggctccgtc atatcggaga tctggatgca ctgcaaccgg atctccgcca
     7621 gtccgtgctt gacgccatgg agttgaccag gaacaacgac aagcttgtgc tcacactagc
     7681 gttcaactac ggaggccgcc aggagatcct tcacgcggtg aagaaaatga ttgaggatgg
     7741 cgttgcaccg gaagatgtct ctgaggaggc gtttgccgag cgactctaca tgcccggcct
     7801 acccgacgcc gacctcatca ttcgcacctc gggcgagtgg cgcctgagca actttctgct
     7861 ttggcaggga gcgtatgccg aactcttctt cactccgaag ctttggccag acttccagcc
     7921 agacgacctg actgaagctg tcgtcgacta cagccagcga gagcgtcggt tcggagcaat
     7981 cgcgcctccg tccaacggat agcgccgtgc gcgagcgatc gatttccgcg ctcggtattg
     8041 taatcctggg gcttgtcccc gcgctcgttg gcggagtgat cttcgcgttc gcattcaccg
     8101 tagttactgt tatcgcctac cgtgaactcc tgcccctgct ccatatcaga cagcgtcggc
     8161 ttgaagtggt cggctatctg ctcatcggac tggccggctt tctggcctgg ctctatcccg
     8221 acggccgtta tctgcccctc gtggttgcat gctgcatgct tgttccactc acctccatcc
     8281 tccatcctcg ttcggatact cgtgacactg gggattgggc cacctgcacc ggagcggtgc
     8341 tatacctgat gcttcccact tttgcggcga tcgcacttcg aggaactgaa ggttccgttg
     8401 accgtcagtg gttccaatcg ctcgctgacg cgatgccgag ttcaggtgcg acggctgagg
     8461 gccttgggct atttctcacc gccttactca tcacctggct ctccgacacc gctgcatatc
     8521 tggtcggcaa atcagtcggc cgcacgaaac tcatccctcg cgtgagcccg aacaagacgg
     8581 ttgaaggcgc catcggcggg ctgattgcag ccggaaccac gtcagtggtg tgtgtggtgg
     8641 ggtttggact tgatgttcac ccactcaccg ccctcatcgt cggcatcctt atcggcgcga
     8701 caggcatggt cggcgatcta agcgaatcga tgctcaagcg ccgcgcgggc gtaaaagaca
     8761 gtggcaattt gatccccggt catggtggca tgctcgaccg cattgatgcg ctcatctttg
     8821 tcgtcacggt cacctgggcg ttgctcccgg cgttgcgcta gctacattgg gtcgatcacc
     8881 acggaggatt cgtcatgacc ggtatcgcgc tgctaggctc cacaggctct attggtgagc
     8941 agacgctgga cgtcattgca acgatggaag accgcttccg ggtggtaacc atggccgccg
     9001 ggctgaggca cgacctactg tcacgtcagg tcgcccgatt tcacccggac atggtcgtct
     9061 ccgcccacga atcaccggat ggaaaccgac cctcaccgca gcaattgatc gaggcagcga
     9121 ctcaccctga tgttgacatt gttgttgttg ctacgtcagg ccacgacgct atccctgccg
     9181 tcatagcggc actcgaggcc ggcaaagcca tcgctctggc caacaaggaa gcaa
//
LOCUS       DASYWM010000240         5195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_235447, whole genome shotgun sequence.
ACCESSION   DASYWM010000240 DASYWM010000000
VERSION     DASYWM010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235447"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VGR29_09770"
     CDS             complement(<1..504)
                     /locus_tag="VGR29_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073916.1"
                     /translation="MSTSNSSRGDDAVIYIDSSVEQEAWLIATFGPTDQWSIASQTWH
                     TTDMGNQQEMLELALPSGEHVEVTFAEASPDELLAGEGQDRTGKIEELMQRALEFSEA
                     NPPHHPGTLPRFPVPSASYRDTIAVPMTVLAVDDAGARGLYAPPRQVGISNKDGSLVG
                     AGEFPGFD"
     gene            complement(501..1178)
                     /locus_tag="VGR29_09775"
     CDS             complement(501..1178)
                     /locus_tag="VGR29_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073917.1"
                     /translation="MGPVEPAPIRRDIAETNLGTTVNPYVLTGLVMCIVIIIALIGTS
                     YLAVYFTRRAKVDLEQLLTPLAELLDGEVEIEEAEVKGRWRGYLVMARMANAAAGTVR
                     IFQIDMIDPAGGDGWNFVYSRPRKGHPEPEIDFQSTNQSIRAALAGLEAFRLAPLEPG
                     KRDWLQLEYSPDAGYVRLARPMHGRNEIPAPLDFQVDLDYLVQLCDENRALQELNRGG
                     GEEGVKS"
     gene            1199..2482
                     /locus_tag="VGR29_09780"
     CDS             1199..2482
                     /locus_tag="VGR29_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2073918.1"
                     /translation="MVVILYNAVQPGVRSGLNILTVGHLMRLTWHFGLGRELGLAFWA
                     LTFFEASYGSYASVWPLWIERLGAPITTVGFVLGAAGVVRPFVVGGGSWLTDHIDTKK
                     LLIGARSLAILGLVIAAFARSWELLLLTVVTNAVGELVFPVIHSHVTEHAGNNAARAF
                     NMVITIGPASALIVTPFIAGAIIAVFGIPGAILFGALMSALGTLCVAIIGFQRHPPST
                     DASAPASYRVVLQHTGARNIVILHGGTVLALAMGSALIPNFLKDERGLSPEVISILSA
                     GAAVGTVTFGFISMRNRKLRSAPVLGAAIATGLTATGYFLFAILDVFPAIALAYVLRG
                     GLFSTWALFLAGMGEIAPRPLRTRAFALVEILGGSAISFGPVIASVLYRVEPPLFLIV
                     SGIASYGMCAVLLWKHGALARARKAEQDAMLAEIV"
     gene            2616..4094
                     /gene="gcvPB"
                     /locus_tag="VGR29_09785"
     CDS             2616..4094
                     /gene="gcvPB"
                     /locus_tag="VGR29_09785"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEV2073919.1"
                     /translation="MAVEPLIYELGKPGRRAIRFPAPDVPETDLPEELVRDELAWPEV
                     SEIDVIRHFTRLSQKNAAIDLNMYPLGSCTMKYNPKINEAVARYPGFARLHPFQPEQT
                     VQGALELMYELQEMLGEISGFNAVTLQPAAGAHGELTGCLIIHAYHRERGDDMRTKML
                     IPDSAHGTNPATARMAGFSVITVPSDERGNCDIDKVRELVGPDTAGIMITNPNTLGLW
                     DEHIHEVIDVVHEAGGLVYNDGANFNAILGIARPGDLGVDIMHYNLHKTFSTPHGGGG
                     PGSGPVGVRADLIEYLPGPHIRKEGEDTDAVYSWYQPEKSIGRLHSFNGNFGMHVRAY
                     TYIRMHGAEGLRQISEDAVLNANYLLALLGDRFDLHYERTCMHEFVLSGTRQKKQGVR
                     TVDMAKRMLDFGVHPPTTYFPLIVPEALMIEPTETESKESVDYFANVMLRIADEAENT
                     PEVVTSAPHTTEYKRLDEVGANRTLNLRWEPERARAEPVAAD"
     gene            complement(4238..4912)
                     /locus_tag="VGR29_09790"
     CDS             complement(4238..4912)
                     /locus_tag="VGR29_09790"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEV2073920.1"
                     /translation="MAMTLTPQQFDALRRIDSPTIANAIETLKIRPRVTGYVGYDIRC
                     IFPDLPPTVGYAVTCTVDSTTEGREGIGFNRLYEVLAKAPKPAIVVMQDVGQDRLHSC
                     HAGEVMSTRMKGLGAVGILTDGGLRDVREVGALGGFQYFCAGLVVSHGNPICVSVGDE
                     VSISGMRVQPRDLLHGDVNGVVHVPDEVAGDVVEAAYRVWEQEGETMRSLARSAAPAG
                     SEELKH"
BASE COUNT         1018 a         1623 c         1481 g         1073 t
ORIGIN      
        1 gtcaaatcca ggaaactccc ccgcgccaac gagactcccg tccttattgc tgatccccac
       61 ctgacggggt ggtgcataaa ggccgcgagc tccggcatca tccacagcca ggacagtcat
      121 cggcaccgca atcgtgtcac ggtatgacgc tgatggcaca gggaaccgcg gtagcgttcc
      181 tggatggtgc ggcggattag cctccgagaa ctccagtgcc cgctgcatca gctcttcgat
      241 cttgccggtg cggtcctggc cctccccggc cagcaattca tcgggagagg cctcggcgaa
      301 ggtcacctca acgtgctcac cggagggcaa tgcgagttca agcatttcct gctggttgcc
      361 catatcggtg gtgtgccatg tttgcgatgc gatggaccac tgatcggtag ggccaaaggt
      421 agcgatcagc cacgcctcct gctcgaccga tgaatcaata tagatgactg catcgtctcc
      481 gcgggatgag ttcgaggtac tcacgatttg actccttctt ccccgccacc ccggttaagc
      541 tcctgcagcg ctcgattctc gtcacataac tggacgaggt aatcgagatc cacttggaaa
      601 tccagtgggg ctggaatctc gttgcgtcca tgcatcggac gagcaaggcg gacatatccg
      661 gcatccgggc tgtactccag ctgtaaccag tcccgcttgc caggctctag tggtgccaac
      721 cggaaagcct caagacccgc gagtgcagcg cgaatggact ggttggtgga ctggaaatct
      781 atctctggct caggatgacc cttgcgcggt ctcgaataga cgaagttcca gccatcgccg
      841 ccggccgggt cgatcatatc gatctggaat atccgcacgg tgcctgcggc tgcattcgcc
      901 atcctggcca tcaccagata ccctctccag cgtcccttca cttccgcttc ctcgatctcg
      961 acctcaccat ccaaaagttc tgccaggggc gtcagcagtt gttcaagatc aaccttggcc
     1021 ctgcgcgtga aatagacagc gagataggag gtgccgatga gcgcaatgat gatgacgatg
     1081 cacatcacca gaccggtaag gacatatgga ttcacagtgg ttcccagatt cgtttcggca
     1141 atatcccggc gaataggcgc cggctcaacc ggtcccattc tacgggatgc gcgtcgtcat
     1201 ggtcgtcatt ctgtacaatg ccgttcagcc cggcgtccgg tcgggattga acatcctgac
     1261 cgttggacat ttgatgagac tcacgtggca cttcggcctc gggagggagc ttggactcgc
     1321 cttctgggca ctgacttttt tcgaggcgag ttacggttcg tacgcgagcg tatggcccct
     1381 ctggatcgag cgcctcggcg cgccgataac tacggtcggc ttcgtgcttg gtgccgcagg
     1441 cgtggttcgc cccttcgttg tagggggcgg ctcctggttg accgaccaca ttgacacgaa
     1501 aaagctcctc atcggagccc gatccctcgc catactggga ctcgtcatcg ccgccttcgc
     1561 ccgctcatgg gaactgctgc tgttgacggt ggtcaccaac gcagtcggcg agctcgtctt
     1621 ccccgttatc cacagtcacg tcaccgaaca tgcgggaaac aacgcggccc gggcgtttaa
     1681 tatggtgatt acaatagggc cagcgtcggc attgatcgtg actccattta ttgctggcgc
     1741 catcatcgcg gtctttggca tacccggtgc cattttgttc ggcgccctga tgagcgccct
     1801 cggtacattg tgcgtggcca tcatcggttt ccagcgacat cctccttcta ccgacgccag
     1861 tgcgccggca agttaccggg tggtcctgca gcatacaggg gcccgaaaca tcgtcatcct
     1921 gcatggaggc acggtgctcg cgcttgcaat gggatcggcg ctcatcccca atttcctgaa
     1981 ggacgagcgc ggcctatcgc ccgaagtgat ctccatcctt agcgccggtg cagccgttgg
     2041 cacagtgacg ttcggtttca ttagcatgcg caaccggaag ctgcgctccg cgccggtcct
     2101 tggcgccgca atcgccacgg gtttgaccgc aacaggatat ttcctgttcg cgatactgga
     2161 tgtcttcccc gccatcgctc tggcttatgt gctgcgcggc ggacttttct ccacgtgggc
     2221 gctctttctc gccgggatgg gcgagatcgc tcctcgtcca ctcagaaccc gcgcctttgc
     2281 tctcgtcgag atccttggag gcagtgcaat ctcgttcgga cccgtgatcg catccgtctt
     2341 gtaccgcgtc gagcctcctc tcttcctcat cgtctccggc atcgcgagct acgggatgtg
     2401 cgctgttctt ctgtggaaac atggcgctct cgcccgagcg cgaaaagcgg aacaggacgc
     2461 catgttggcg gagattgtgt gagccccgtt gtacggaccg atcgtgctac catctcatgt
     2521 ggtcggcgcc acggtgttgc gctggacccg tcaggctccg atcattcatg accatgtttg
     2581 aaactcacca gtttgcaccg acgagggaac cttccatggc ggttgagcca ctaatctacg
     2641 aacttggcaa gcccggacgc cgggccattc gattccccgc ccccgacgtt ccggagaccg
     2701 acctcccgga agaactcgtg cgtgacgaac tcgcttggcc cgaggtgagc gagatcgacg
     2761 tcatccgcca tttcacccgg ctcagtcaga agaacgcagc gatcgacctc aacatgtatc
     2821 cactcgggtc gtgcaccatg aagtacaacc cgaagatcaa tgaggcggtc gctcgctacc
     2881 ctggctttgc acgtctccac ccctttcagc cggagcaaac agtgcagggc gcgctggagc
     2941 tgatgtacga gctccaggaa atgctcggcg agatttcggg gttcaacgcg gtcaccttgc
     3001 aaccggcagc tggtgctcac ggagagctca caggctgtct catcatccac gcttaccaca
     3061 gggagcgtgg ggatgatatg cgcacgaaga tgctgatccc cgattccgca cacggtacca
     3121 acccggccac cgcacggatg gccgggttct ccgtcattac cgtgccctcc gatgaacgcg
     3181 gtaactgcga catcgacaag gtcagggagc tggtaggtcc ggatacagcc gggatcatga
     3241 tcaccaaccc caacacgctt ggtctttggg atgagcacat ccatgaggtt atcgatgtcg
     3301 tccatgaggc cggtgggctc gtgtacaacg acggcgcgaa ctttaacgca atccttggga
     3361 tcgcccggcc cggcgacctc ggcgtcgaca tcatgcacta caacctccac aagacgttct
     3421 cgactccgca tggtggcggt gggccgggat ctggaccagt cggcgtccgt gccgacctga
     3481 ttgagtactt gccgggaccg catatccgca aggaaggcga agacaccgat gctgtctact
     3541 cctggtatca gcctgagaaa agcattggtc ggctgcacag cttcaatggc aatttcggaa
     3601 tgcacgttcg cgcctacacc tacatccgga tgcatggtgc cgaggggctg cgacagatca
     3661 gcgaggatgc cgtcctcaac gcgaactact tgctggcact gttgggcgac cgtttcgatc
     3721 tccattacga acggacctgc atgcacgagt tcgtgctgag tggaacgcga cagaagaagc
     3781 agggggtgcg cactgtggac atggccaagc gaatgctcga cttcggcgtc catcccccga
     3841 caacctactt cccgctcatc gtgccggaag cgctcatgat cgagccgacg gaaacagagt
     3901 ccaaggagtc cgtcgactac ttcgccaacg tcatgttgcg gatcgccgat gaggcggaga
     3961 acacgccgga agtcgtgacg tcggcaccac acaccaccga gtacaagcgc ctggacgagg
     4021 tcggtgccaa ccgcacgctt aacttgcgct gggagccgga gcgagcgcgg gcggagcctg
     4081 ttgccgctga ctagacgtta cgtccagaac acgatagaag cagaaaggac tccggagaat
     4141 ctcgtctccg gagtcctttc tggttctgaa gggtacgcac gtgcgcgaga acgaccgagc
     4201 taaccctttg cttcgctaat ttcgcctgga actcggctta gtgtttcagt tcctctgatc
     4261 ctgcaggggc agcagaccgg gcgaggctcc gcatcgtttc gccttcttgc tcccaaaccc
     4321 gatacgcagc ctccaccacg tcacctgcga cctcgtccgg cacatgaacg acgccgttga
     4381 catcgccgtg gaggagatcc ctcggctgaa cccgcatacc ggagatggac acctcgtcac
     4441 cgacgctgac gcagatcgga tttccatggg acaccaccaa cccggcgcag aagtactgga
     4501 agccaccgag ggctcccacc tcacgcacgt cccgcaggcc gccatcggtc aggatcccga
     4561 ccgcacccag tcccttcatg cgtgtggaca tcacttcgcc agcgtggcaa gagtggagcc
     4621 gatcctgacc tacgtcctgc atcacgacga ttgccggctt cggtgccttc gcaaggacct
     4681 cgtacagcct gttgaaaccg atgccctctc tcccctcggt tgtgctgtcg accgtgcacg
     4741 tgaccgcata accaacggtg ggaggcaaat cgggaaagat gcagcgaatg tcgtagccaa
     4801 cgtagccagt cacccgaggg cggatcttga gtgtctcaat ggcgttcgcg attgtcggac
     4861 tatcgatccg gcgcagtgcg tcaaactgct gcggtgtgag cgtcatggcc atctctcgtt
     4921 cccttcaatg actttccaca tggtcgccag cggccatttt ccaggcaatc tgctccgccg
     4981 tcaatcaccg gagcctgctc cgagtgctct tcatcattag cgtattgcgg cttgtgtgac
     5041 tgggagtgct ggcaacggtc cgcaagataa ggcaacaagc ggcggctcaa catcagttgg
     5101 ggtccgccaa ccgcgacgcg ggaacatcag ggcccctcta acagtcgcag taatggacgg
     5161 tctatggtgg ccgtccatta ctgcgactgt tagag
//
LOCUS       DASYWM010000241         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_461868, whole genome shotgun sequence.
ACCESSION   DASYWM010000241 DASYWM010000000
VERSION     DASYWM010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461868"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(590..859)
                     /locus_tag="VGR29_09795"
     CDS             complement(590..859)
                     /locus_tag="VGR29_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073921.1"
                     /translation="MSQDDVQRTPPWMKKQASDEPGLDDRSDVTHVTASRAEDEAPAA
                     GAPSTSDNDPSETVGTGTSIALGCVAGTILLIVFGLIFLALLALL"
     gene            1365..2171
                     /locus_tag="VGR29_09800"
     CDS             1365..2171
                     /locus_tag="VGR29_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV2073922.1"
                     /translation="MQLMTRERIEDLLRITSDEWRSRIEEWSTRSEIVLLDEVELLLC
                     RAVCAWAGVPLNEHDVQTRTWEFSAMITGAGGVGPTTWKALFLRQRTERWVQSVIREI
                     REGKREPPESSAAYVIATYRDLSGELLRADVAAVELINILRPTVAVARFIAFAAHALY
                     HYPESRRFIDSAADEETELFAQEVRRFYPFFPFIGGCVREPFDWQGHHFESGTWVTLD
                     VYGTHRDPRLWENPESFQPDRFRGWDGSAFNFIPQGGGITSQRTAARASG"
     gene            2168..2320
                     /locus_tag="VGR29_09805"
     CDS             2168..2320
                     /locus_tag="VGR29_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073923.1"
                     /translation="MTIALLKQAAAYLANAMTYTVPEQDLSINLRIMPARPQSGFIMK
                     HVRPVA"
     gene            complement(2474..3016)
                     /locus_tag="VGR29_09810"
     CDS             complement(2474..3016)
                     /locus_tag="VGR29_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073924.1"
                     /translation="MRRRGCLWGLTGVIGLLALCCVLAFFVGLPRLQDSIRDEVAEQL
                     STQVARQLDAQVSGGVAVGAGEYRLSLSDIEQQIAADADPSTVETLDLRADGNELVLS
                     IATDWETIEYRGIPTAGPTGELEMADMRSDGGGIDYLLPPDQVGGAIERGVNSYLQAR
                     GLQLQDIQLDRNDLILNLVE"
BASE COUNT          658 a         1055 c          944 g          645 t
ORIGIN      
        1 ctactggtct tccgtgaccc atcagcattg gtcaaccgtc tcgcacctgg tgcacggacc
       61 tgacgtgaat cccgtctgct tccagaaggc gctgctgggt cgctcccgct cacgtccacg
      121 cgccctgccg gtcgtcgttg ccgggttctc catctcgggc atcaggttcc ggcgtcgcca
      181 ccggactggt tcccgcgggc gagccacact cgctacgaac atgcatgcaa gcgccacgcc
      241 agagattccg ggctacgaag ccgggcgcgt tcgtctcatc gcctgcgata ccgtgttctc
      301 cggaattcca ttacatgtgg gcgcatcggg atggacgctg ttgcaggctc acgtctcggc
      361 cgtaagaacg gcagtggacc ggaacacttc ccagcggttc catgtcggcc gtcaaccgtc
      421 aggttgctgg cacacggtca ttgctgcagg atcagcaccc aggccccagt tcttgcacgg
      481 ccatcccgct gcgcgcctgg gcgcaggacc agcaagaaca cgaagtcaaa ggaagtgtat
      541 cagcaaacgg acatgggaat gatcccgacg tccaccaact acttcctggc tagagcaacg
      601 ccagcaacgc aagaaagatg aggccaaaga caatcagcag gatcgtccct gcgacacacc
      661 caagcgctat cgaggtaccc gtgcccacgg tttcgctagg atcattgtct gatgtagatg
      721 gagctcctgc cgccggagcc tcgtcttcgg cgcgcgacgc cgttacgtgg gtgacgtccg
      781 accgatcatc aagccccggc tcgtcggaag cctgcttttt catccagggc ggtgtccgct
      841 gtacgtcgtc ctgactcacg tataccctcg tatcaccggc cacgggtgcg accgatcggg
      901 gcgcatcccg gaattcactg ctccggcaag gatagcatcc ggccggtgct gctccactcc
      961 caaaccgtcc ttccattggt acaaagcttg cagcgatgcc aataacaaga gacttggatc
     1021 gcccggtgaa gcattcgcct actggtgctg cctcatccta ccggaaggcc tgcgatgaac
     1081 atcccccgcg ataagaccgc cgacagttca ctcatgctcc tcttacaggg ataccgcttc
     1141 ttgccaagcc ggtttcaaag gcaccgatcg gatatcttca agacacggct catgttgcag
     1201 cgggtgatag gtgtccgggg ggagacgcgg cggagatgtt ttaccagcca ggtcgtttca
     1261 ctcgacacgg tgcactacca ccccagacgc tgcggctcct gcaagacacg ggtagcgtcg
     1321 cgatgtagaa cggcaaagcc caccgccatc gaaaggcaat gtttatgcag ctcatgacgc
     1381 gagagcgcat cgaggacctg ctgcgaatta cgtcggacga atggcgatcg cggatcgagg
     1441 agtggtccac gaggagcgag atcgtgctgc tcgatgaagt cgaactgctg ctctgccggg
     1501 cggtctgtgc ctgggcagga gtgccgctca atgagcatga tgtccagacg cgtacgtggg
     1561 agttctccgc catgatcaca ggcgccggtg gcgttggtcc tacgacttgg aaggcgctct
     1621 tccttcggca acgaacggag cggtgggtgc agagcgtgat cagggagatc cgcgagggta
     1681 agcgcgagcc gcccgaatcc tccgccgcct acgtcattgc cacataccgc gacctgagcg
     1741 gagagttgct ccgggcagac gtagccgccg tcgaacttat caacatcttg cgccccaccg
     1801 tcgccgttgc ccggttcatc gcctttgcag cgcacgctct ctatcactat cccgaaagca
     1861 ggcggttcat cgattcggca gcggatgagg aaactgagct gttcgctcag gaggtgcggc
     1921 gattttaccc gttctttccc ttcatcggtg ggtgcgtgcg tgaacccttc gattggcagg
     1981 gccaccactt cgaatcaggc acctgggtca cgctggacgt gtatggaacg caccgcgacc
     2041 cgcgactctg ggagaacccc gagtcctttc agcctgatcg cttccgcggc tgggacggca
     2101 gcgccttcaa cttcatccct cagggagggg ggatcacttc acaacgcacc gctgcgcggg
     2161 cgagtggatg actattgccc tgctcaagca ggcggcggca tacctcgcga acgctatgac
     2221 atacacggtg ccggagcaag acctcagcat caatctgcgg atcatgccgg cccggccgca
     2281 gagcggcttc atcatgaagc acgtgcgacc ggtggcctag cgggtctggg tcggggaccc
     2341 agacggagga aaatcgacga gatcattgcc acagtgcgac gtgcgtccag tccaaacgct
     2401 tttatggttc gaatcgaagc cttgcagtgt gccttatacg gggaaggcag gacggcaggt
     2461 tgtgtcgcga cacctattcg acaaggttaa gtatcaaatc gttccgatca agctggatat
     2521 cctgcagctg cagtccccgc gcctgcagat agctgttgac gcccctctcg atcgcgccac
     2581 caacctggtc cggtggcaag aggtaatcga ttccgccacc atcgctccgc atgtccgcca
     2641 tctccagctc ccccgttggg cctgctgtgg gaataccacg atattcgatg gtctcccagt
     2701 ccgtagcaat cgagagcacc agttcattcc catcagcacg cagatcgagc gtctcgaccg
     2761 tcgacggatc ggcgtctgcg gctatctgtt gttcgatatc gctgagggag agccgatatt
     2821 cccccgctcc caccgccaca ccccctgaaa cctgggcgtc gagctggcgg gctacctgcg
     2881 ttgagagctg ttcggcaact tcgtcgcgta tggagtcctg gagacgcggc agccccacga
     2941 agaatgccag tacgcaacac agggccagaa gaccaatcac accggtcaat ccccacagac
     3001 agcctcgtcg acgcattcca tctacctttc acttacacgc tccgatttca cgcgcaaggc
     3061 aaccccaagc ctatgcaggc tctaacgtct gagaaacgtg gactggccga tagtgagcaa
     3121 tcagtcgctc actatcggcc agcctgtcac gctgttctgc cccctctgga gtcgttctac
     3181 tcggtgcggt tgaagaccat caggtcctcc cgtctcggat caaggcccac cagctgctcc
     3241 ggagcatctt cacccgcagg accgtacaat gtcacccatc catttgtcgt ggtgccatag
     3301 gg
//
LOCUS       DASYWM010000242         7062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_536396, whole genome shotgun sequence.
ACCESSION   DASYWM010000242 DASYWM010000000
VERSION     DASYWM010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536396"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VGR29_09815"
     CDS             complement(<1..383)
                     /locus_tag="VGR29_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073925.1"
                     /translation="MEFDTIIRNGMVVDGTGSTARQTDVGILGDRIEAVDELDEAAAA
                     NIIDATGQVVSPGFIDVHVHSEVALANPQDQRRYGSVLQGVTTHLTAPDGFGWAPLDH
                     EEAASLWRSTLFSHGEANLSPGWSTV"
     gene            complement(427..1557)
                     /locus_tag="VGR29_09820"
     CDS             complement(427..1557)
                     /locus_tag="VGR29_09820"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV2073926.1"
                     /translation="MARSPCHLENVLVITALIGQSLDVLDTPALLVDRDALNHNIHVM
                     VSAVQQHEVGWRPHSKAHKSPAVAHLQLAAGAHGITCAKTSEAEVYAASGVRDILIAN
                     QVVGPIKTRRIAHLAHHADIAVAVDSFDNVLEHEAAASEAGTSPRLVIEINSGMNRAG
                     IEPGEPSLNLARRIAALKHVRFGGIMTWEGHTLSIKEPERRQEAILSALAPVLQTVTA
                     IRAAGIEVPIVSCGGTGTFQVAAGIVGVTEVQAGGGIFGDRFYRDLGVPVYPALSLLV
                     IVTSRPTPYRLIIDAGRKSVDPSNVIPEVIGLQNVTSLAFSAEHGTVEMSAPNATVKV
                     GDRLRLNIGYSDQAVHLHEQLIVLSGETIDAVWPTYARGRLQ"
     gene            1773..3614
                     /locus_tag="VGR29_09825"
     CDS             1773..3614
                     /locus_tag="VGR29_09825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEV2073927.1"
                     /translation="MVEGSGVDARVTVVMPTFNQAMFLRRAVDSLIAQTFQSWRLVIV
                     DDGSTDETSSIVEPYGANPRIDVIHLTGNGGLGRALNVGLDRASGDLIAYLPSDDVYH
                     AEHLATLVACLDRRPEAMLAFSGVRHHYNRVSSGLIPGEPLQLVQVLHRRTSDRWMER
                     HELVTDDLDRMLWSRLHQQGEFSETGEDTCEWVDHPGQLHKLLREPTGGINPYRDTFG
                     VREPMRFHTTVGNRIDEISRYQRFRNRPATPMAPDGLKILLVGELAYNADRVLALEEL
                     GHRLYGLWSPAKDIDWFNTVGPVPFGHVEDIPRYGWQDAVRSIKPDVIYALLNWQAVP
                     FAHQVLTENPGIPFVWHFKEGPFICLERGTWPQLVDLTEQADGLIYSTPEMGEWFASI
                     VPGIGDHRSMILDGDLPKRDWFEGQRAERLSGKDGEIHTVVPGRPIGLHPETVAELAR
                     HRIHLHFYGDYTHGQWRAWIDRTNTMASGYLHLHHHVDQEHWLPEFSRYDAGWLHSFS
                     STNRGDIRRANWDDLNLPARVATLAVAGLPMIQADNTGSIVATQALARQRQLGPLYSS
                     IDDLAAQLHDEPRMKELGESVWNQRQDFVFDTHAERLVAFFREVIDS"
     gene            complement(3708..4811)
                     /locus_tag="VGR29_09830"
     CDS             complement(3708..4811)
                     /locus_tag="VGR29_09830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEV2073928.1"
                     /translation="MQSYGGRIPDVRRIAVLRANAIGDFLFTLPALDALRAAYPDAEI
                     VLLGQRWHEAFLAGRPGPVDRVIAIPRCRGVGEPEDFVNDTDALDQFFARMVEERFDL
                     AIQLHGGGRYSNPFVHRLQSRFTVGLKSAEAAPLDRWMPYVYFQHEIPRYLEVVAMVG
                     AGMVTLEPRVTLTDGDIREAAIVVPLDDAPLVVLHPGAGDPRRRWPPEKFAAVGDALA
                     AAGARVAVVGAREDERQVVEAVVDTMRASAENLWEKLSLGGLAGLLDRCAVVVSNDSG
                     PLHLAAAVDTPTVGIYWCFNLVNAGSLSRTLHRPQLSWRLECPVCGINCLADHCDHDA
                     SFVADLPVEQVIASALELLSGQAQSSDGVNAVP"
     gene            5093..6592
                     /gene="rfaE2"
                     /locus_tag="VGR29_09835"
     CDS             5093..6592
                     /gene="rfaE2"
                     /locus_tag="VGR29_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEV2073929.1"
                     /translation="MDDLDRSPAEIVRSFPGLRVLVIGDAMLDTYLEGTATRLCREAP
                     VPVVVSQSEQRCPGGAANTAANVRALGAEVDFVGIIGNDPASARLRTTLNDAGVSDRS
                     LVVDDNVTTVHKSRIIADDQYLVRFDEGDLTTCSSTARQAVLDRVEDCYSLCDLVILC
                     DYACGTVNDEIVALLRRLRAERPRILAIDSREVHRFAQAGATILTPNLQEARAAVSLD
                     QTLKGQEPDEIARQLRHVLDAEHIVVTMAGDGVLLIDTNGQTNRLPAYPVARAGDVGA
                     GDSFTAATALALATGAGIAQAAQIGIDAASIACTKRRTAVVTNRELLQRVSINSVAST
                     CGPLSLKEVATRLDAERYAGRRIVFTNGVFDILHAGHVRLLQQARQLGDVLVVGINSD
                     ASVRRLKGEQRPINGEQDRLSLIAALDAVDYTVIFTEDTPAEVIQALRPDIHVKGGDY
                     TPETLPEIDAVREVGARVEILPLVDGLSTTNVIGRIASMASRDPAEQAI"
     gene            6856..>7062
                     /locus_tag="VGR29_09840"
     CDS             6856..>7062
                     /locus_tag="VGR29_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073930.1"
                     /translation="MRRLPALDRLEYFQGYPGIPEVAHGREPGSAFLADAQESRYDLA
                     IQMHGDGKLSNGFIAGLGAKVTVGY"
BASE COUNT         1424 a         2178 c         2117 g         1343 t
ORIGIN      
        1 acggtagacc agcctggcga tagattcgct tctccgtgcg agaagagtgt ggaccgccag
       61 agacttgcgg cttcctcgtg gtcgaggggc gcccagccaa acccgtccgg cgccgtcagg
      121 tgagtcgtca ctccctgcag cacgctgccg tagcgacgct gatcctgcgg attcgccagg
      181 gccacctctg agtgcacatg gacatcgatg aagccgggcg agacgacctg gcccgttgca
      241 tcgatgatgt tggccgccgc tgcctcgtcg agttcatcga ccgcctcgat ccggtcgccc
      301 aggatcccaa catccgtttg gcgcgcagtc gaacccgtgc cgtccaccac catgccgttt
      361 cgaatgatcg tgtcgaactc catcgtatca ccctcgctat ctggttccaa ttcgaagagc
      421 ggatggctat tgcaggcggc cacgcgcata tgtcggccag acagcgtcga tcgtctcgcc
      481 gctcaggaca atcaactgct cgtggaggtg gacggcctga tcgctgtaac cgatgttcaa
      541 ccgcaaacgg tcccccacct tgacggtcgc gttcggagcg ctcatctcga cggttccatg
      601 ctccgcagaa aatgcaagac tggtgacatt ttgaagaccg atcacctcgg gaatgacatt
      661 ggaggggtcc accgactttc gccccgcgtc gatgatgagg cgatacggtg tcggacgact
      721 cgtcacgata accagcaacg acagcgccgg gtacaccggt acgccaagat ctcgatagaa
      781 gcgatcgccg aaaatgccgc cccccgcctg cacttcggta accccgacta tgcccgcggc
      841 gacttggaat gtcccggtcc ctccacagga aacgatgggc acctcaatgc cagccgcgcg
      901 gatggcggtg acggtctgga gtacgggcgc gagcgccgac aggatggcct cttgccgcct
      961 ttcgggttcc ttgatcgaga gcgtgtgccc ctcccatgtc atgatcccgc cgaaccgaac
     1021 gtgtttgagc gcggcaattc gcctggcgag attgagcgag ggctctccag gctcgatacc
     1081 cgcccggttc atcccgctgt tgatctcaat caccagccgc ggcgacgttc cggcttctga
     1141 cgcagccgcc tcgtgctcga gcacgttgtc gaatgaatcg accgcgacag cgatgtcagc
     1201 gtgatgggca aggtgggcga ttcggcgggt cttgattggc cccacgacct gattggcgat
     1261 caggatgtcg cgaacaccgc tagcggcgta aacctccgcc tcactggtct tcgcacaggt
     1321 gatcccgtga gcacctgctg ccaactgcag atgtgccaca gcaggtgatt tgtgcgcctt
     1381 gctgtgtgga cgccatccga cttcatgctg ctgcactgcg gaaaccatca catgaatgtt
     1441 gtggttgagg gcatccctgt cgacgagcag cgccggagta tcgagcacat cgaggctttg
     1501 tccgataagt gcggtgatca cgaggacatt ctccaggtgg catggggaac gggccatcgt
     1561 ttctcgacgg cacctctgcc gagcattgta ccgacgatct cctttggagc aattgcccca
     1621 gcagcggctt acctcggctg agggtggaac tgggcccaaa tgcggctggt acactcgcgg
     1681 cagcagaagt cggaccggct ccgttggtac ggtgccggtc ctggtcggca gggccagttc
     1741 gagagagatc ggccagaaag gaagtcgcag gaatggtgga aggatcaggc gtggatgccc
     1801 gcgtcactgt agtcatgccg accttcaacc aggcaatgtt cctgcggcgt gcggtcgata
     1861 gcctcattgc gcagaccttc cagtcttggc gtcttgtcat cgtcgacgat ggctcgacag
     1921 atgagaccag cagcattgtc gagccctatg gggctaatcc ccgaatcgac gtcatccacc
     1981 tgacgggaaa tggcgggcta ggccgcgccc tcaacgtggg actggataga gcatcgggag
     2041 acttaatcgc ctacctgcca tcggacgatg tctatcacgc ggagcacctc gcgacgctcg
     2101 tcgcctgcct cgaccgccgc ccggaggcta tgttggcctt ctccggcgtc cgacatcatt
     2161 acaaccgggt ttcctccggg ctaattccag gcgagccgct ccagctcgta caagtgctcc
     2221 accggcgaac cagtgaccgc tggatggagc gtcatgagct agttacggac gatttggacc
     2281 gaatgttgtg gtcacggctc caccagcagg gagaattttc ggagactggc gaggacactt
     2341 gcgaatgggt ggatcaccct ggccagcttc acaaactgct ccgggaacca acgggtggaa
     2401 ttaatcccta tcgcgatacc ttcggggtgc gtgagccaat gcggttccac actacagttg
     2461 gtaacaggat cgatgagatt tcgcgatacc aacgattccg gaatcgcccc gctaccccga
     2521 tggcacctga cggcctcaaa atcctgctcg tgggggagct cgcatacaac gcggatcggg
     2581 ttcttgccct ggaggagctc ggtcaccgtc tctatggcct ctggtcccca gcgaaggata
     2641 tcgactggtt caacactgtc ggcccggttc cctttgggca tgtcgaagac attccccgtt
     2701 acggatggca ggatgccgtc cgaagtatca agccggatgt gatatacgcc ctcctcaact
     2761 ggcaagccgt tccttttgcg catcaggtcc tgacagaaaa ccccggcatc ccctttgtct
     2821 ggcactttaa ggaggggcca ttcatttgcc tggagagagg gacgtggccg caacttgtag
     2881 atcttacgga gcaggctgac ggcctgatct actctactcc tgagatgggc gagtggttcg
     2941 cgagtatcgt tccgggaatc ggtgatcaca ggagcatgat cctcgatggt gatcttccca
     3001 agagggactg gttcgaggga caacgtgccg agcgtctatc agggaaggac ggtgaaatcc
     3061 acacggtggt acctggacgg cccataggtc tgcatcccga aaccgtggcg gagttggctc
     3121 ggcaccggat ccacctgcac ttctatggcg attacacaca cggacaatgg cgggcatgga
     3181 tcgatcgtac gaacaccatg gcctccggct atcttcatct ccaccaccat gttgaccagg
     3241 agcattggct tcccgaattc tcacgctacg atgccgggtg gctccattca ttctccagca
     3301 ccaaccgtgg ggatattcga cgggcgaact gggatgacct caacctgcca gcgcgcgtgg
     3361 caacgcttgc agtcgccggg ctacccatga tccaggccga caacacaggc tccatcgtcg
     3421 ctacacaagc ccttgccagg cagaggcaac tgggcccgct gtatagctca atcgacgatc
     3481 tggcggcaca attgcacgat gagccgcgaa tgaaggaact gggggagagt gtctggaatc
     3541 agcgtcagga cttcgtgttc gacactcacg ctgaacggct tgttgccttt ttccgggaag
     3601 tgatagacag ctagtcgatc tccgtatccg gtcgcaaacg cctctccttt aggaagagag
     3661 ccggagacgc atggccgggc cacgacacga tccatctggt ttcgtgctca gggaacggcg
     3721 ttgacgccat ccgaggattg agcttgcccc gaaaggagct caagggcaga ggctatcacc
     3781 tgctccaccg ggagatcggc gacaaaagag gcgtcgtgat cgcaatgatc ggcaaggcag
     3841 ttgatcccac acaccggaca ctccagtcgc catgacaatt gcggccggtg aagcgtgcgg
     3901 ctaagtgagc cggcattgac aaggttgaaa caccagtaga tgcccacggt cggggtatcg
     3961 acggcagcgg caagatgcaa cggccccgaa tcattcgaga caacaaccgc gcatcggtcc
     4021 aggagcccgg ccaaaccgcc gagtgagagt ttctcccaga gattctccgc gctggcccgc
     4081 atcgtatcga cgaccgcctc aaccacttga cgctcatcct cgcgggcacc aaccaccgcg
     4141 acccgtgcgc cagccgcagc caacgcatcg ccaacggccg caaacttttc cggcggccac
     4201 cgccgcctcg gatcgccagc gccgggatgg aggacgacca acggcgcgtc atcaagcgga
     4261 acgacgattg ccgcctccct tatgtcgcca tcggtcaatg tcacgcgggg ctccagcgtg
     4321 accatgcccg cgcccaccat ggcgacaacc tcgagatagc gaggaatctc atgctggaag
     4381 tagacgtagg gcatccaacg gtcgaggggc gctgcctcag cgcttttgag tccgaccgta
     4441 aatcgggact ggagccggtg cacgaaggga ttggagtacc gccccccgcc atgaagctgg
     4501 atagcgagat caaaccgttc ctctaccatt cgcgcgaaga actggtcgag ggcatctgta
     4561 tcattgacga aatcctcggg ctcccctacg cctcggcacc ttgggatagc gattacccgg
     4621 tccaccggac ccgggcgccc ggccaggaac gcttcgtgcc agcgttgccc gagaagcacg
     4681 atttccgcgt caggataggc agcgcgcaga gcatcgagtg ctggcagcgt gaagaggaag
     4741 tcaccgatcg cgtttgcccg caagactgcg atgcgacgaa catcgggaat acgaccgccg
     4801 tagctctgca tcaattgccc tttccctcgt aacatttccc gccgttcctt gtggcaagac
     4861 gctctacgca cgtccggcgc cccggtttgg agcggccggc aataacgacg tggagatgct
     4921 catggatgct tgagaagaag tggtattgta gttgcacggt tgccctcatt gagctggagc
     4981 atacgcttcc ggtgcttcat caacacgcat ctcccaccct ccgcgttgat atgcggcaca
     5041 gggggagaca cgagggagcc ggcgccaaca ggcgatagga gatgccattc tgatggacga
     5101 tctggaccgg agtcccgccg aaatcgtgcg gagctttccc gggttgcgtg ttctggtcat
     5161 cggcgatgca atgctggaca cgtacctgga gggaacggca acccggctct gcagggaagc
     5221 cccggttccc gtggtcgtga gccaatccga gcaacgatgc cccggcggcg cggcgaacac
     5281 cgctgccaat gtacgtgcgc ttggggccga ggtcgacttc gtcgggatta tcggcaatga
     5341 cccggcttcg gccaggctgc gaacgacctt gaacgatgcc ggggtcagcg atcgttcact
     5401 cgtggtcgat gacaacgtga cgacggtgca caagtcacgc ataatcgcag atgaccagta
     5461 tctcgttcga ttcgacgaag gtgacctcac gacttgctca tccactgcgc ggcaagcggt
     5521 cctcgaccgg gttgaggact gctattcact ttgtgatctg gtcatcctct gcgactacgc
     5581 gtgtggcacc gtgaacgacg agatcgtggc gctgttgcgc cgcctgcgcg ccgagaggcc
     5641 gcgcatcctg gccatcgact ctcgggaggt gcaccgcttc gcacaagccg gagcgacaat
     5701 cctcacgcca aacctgcagg aagcgcgggc ggcagtgtcc ctcgaccaga cgctcaaggg
     5761 tcaggagcca gacgaaattg cccgccagct acgacacgtc ctcgatgccg agcatattgt
     5821 cgtcaccatg gcgggagacg gggtcctcct gattgacacc aatgggcaga cgaaccgact
     5881 gcctgcatac cccgttgccc gggctggcga tgtaggcgct ggggattcat tcactgcggc
     5941 gactgcactc gcccttgcaa ccggtgccgg catagcccag gcagcacaga tcgggattga
     6001 cgctgccagc atcgcgtgta ccaaacgacg aacggccgta gtcacgaacc gtgagctgtt
     6061 acagcgagta agcatcaact cggtggcatc aacctgcggt cccctttcgc tcaaggaggt
     6121 cgcgacccgg ctggatgcgg agcgatacgc aggccgccgt atcgtcttca caaatggggt
     6181 cttcgatatc ctgcatgccg gccacgttcg gctcctccag caggcgaggc agctcggcga
     6241 tgtactggtg gtgggcatca atagcgacgc aagtgtccgg cgactgaagg gtgagcagcg
     6301 tccaatcaac ggggagcagg accggctctc cctcattgcc gcgctcgatg ccgtcgacta
     6361 caccgtcatc ttcaccgagg acactccggc agaggtaatc caggccctga ggcccgatat
     6421 ccatgtcaag ggcggcgatt atacgcctga gaccttaccg gaaatcgacg ccgtccgaga
     6481 agttggtgcg cgtgtcgaga tcctgccgct tgtcgatgga ttgagcacga ccaatgtcat
     6541 cggtcgaatt gcctctatgg ccagccgaga ccctgcagag caggccatat gatgttcccg
     6601 gatgggccat cacctgctcc agagcatcca cgtacccctg agtctcattc tcccgaggca
     6661 gaccaaaggt cttcaccgtc gtcccactca ggtgagcctt ggacgaacga cgattggaag
     6721 ccaaagcgaa tcgccgtttt tagggcattg catctcggcg atctcctctg cgccaccccc
     6781 gctttgaggt cgcttcatcg gcggttcatg gacgcggaaa tcaccctgat tggactccct
     6841 tgggccgagg agctcgtgcg gcgcctgcct gcgcttgacc ggctggaata cttccaggga
     6901 tacccgggaa ttccagaggt cgcgcacggc cgagagccgg gatcggcatt ccttgccgat
     6961 gcccaagaga gtcgctacga tctcgccatc cagatgcacg gcgacggcaa gctgagcaat
     7021 ggcttcatcg ctgggctggg cgccaaggtg accgtgggct ac
//
LOCUS       DASYWM010000243         8234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_217977, whole genome shotgun sequence.
ACCESSION   DASYWM010000243 DASYWM010000000
VERSION     DASYWM010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8234
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217977"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /gene="pxpB"
                     /locus_tag="VGR29_09845"
     CDS             complement(<1..700)
                     /gene="pxpB"
                     /locus_tag="VGR29_09845"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEV2073931.1"
                     /translation="MTSPTFFPLSPSAMLMTVGNRIDVNLTYRIARFVELLDKESVPS
                     VLDVIPSYSTILVIYDQTVISCEPLERLLTELWNLAEDGPEARHSRTVAIPVVYGDDF
                     GEDLEDVSAHTGLLPEEVIERHLAGHYTVGALGFAPGFAYLIGLAPELATPRRARPRT
                     HVPAGSIGIGGEQTGTYALSTPGGWNLIGRTPLRLFRPEKDDPFVLRMGDQVRFERIG
                     VEEFHRAEKGVGEQE"
     gene            complement(700..1467)
                     /locus_tag="VGR29_09850"
     CDS             complement(700..1467)
                     /locus_tag="VGR29_09850"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEV2073932.1"
                     /translation="MSTDTLALDINSDVGESYGAFRIGADVELFPLISSANVACGFHG
                     GDPQTMDQTVRALAGLGVAVGAHPSYHDLRGFGRRNIPATPEEIRTDLLYQIGALHAF
                     CQVAGVPLHHVKPHGALYNTATNDRIIADAIVNAVLDLAPDVIFYALPGSELEAAAVA
                     QGLRVAREAFADRAYMSDGTLAPRSLAGAVLEDPASVADRVLRMIGGEEVPTLDGGHI
                     TIAADTICIHSDTPGAVPIASALREALMDAGITIRAL"
     gene            1684..2817
                     /gene="rlmN"
                     /locus_tag="VGR29_09855"
     CDS             1684..2817
                     /gene="rlmN"
                     /locus_tag="VGR29_09855"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV2073933.1"
                     /translation="MTDIIETNLPTSVAVSPESRVVPRRKRLIGFHDLPFDELKVRLV
                     EMGAPAYRAQQMFDWAYGKLVTSYDEMANVPAPIRDSFGETIPLQTMKLVRELVTDNG
                     DTYKLLYQTFDGQLVETVLMLYPDRATVCVSCQVGCAVGCAFCATGLGGLTRNLTAGE
                     IVQQTVDATRRVRQLDRQLTNLVMMGMGEPLQNYAETMKFINIIHDPHGLNFGARRIT
                     ISTSGIIPRIDALADEPYQVNLAVSLHAPNNELRSQLVPINGRYPVEDLLAACDRFTQ
                     KTGRRISFEYALMNGINNSDEIARELGQLLRGRLCHVNVIPFNPVDVLKFERPNAEMI
                     ERFAEIVREYGIPTTVRYSRGVEIAAACGQLRARHQQMEKTAS"
     gene            3000..3977
                     /locus_tag="VGR29_09860"
     CDS             3000..3977
                     /locus_tag="VGR29_09860"
                     /inference="COORDINATES: protein motif:HMM:NF021832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HEV2073934.1"
                     /translation="MTSAHDLRIDMVAVHAVADRLARQEVAPEWDAALHYRATGLDAD
                     ERTAMWLLVLDALNFCFWGQGADPSVRWRVEWKDELTDGYMALVAALMRGVEECQELH
                     SASWLATVKGEDVAHLLRPAEGHPEIPLFEARVHNLRELGRGLLALDSETPATALITL
                     ANGSAVSLVREVVRRFPSFKDIAIWTRTDTGLPGHEVRFYKRAQILVGDLAGGLAPSP
                     LGQFHDLDKLTAFADYKVPQVLRQLGVLRYSDSLAGRITRREHLLPGSADEIEIRAGT
                     IWGCELVRQALDERGRTVSAHELDWLLWTMGQSLPTESEPYHLTPTIFY"
     gene            4854..6098
                     /locus_tag="VGR29_09865"
     CDS             4854..6098
                     /locus_tag="VGR29_09865"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF004852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV2073935.1"
                     /translation="MGSLPVRAIDQHIEVHYSYQVCFTRDIFDLKNDIVRDICSGEDQ
                     DRQRKVLVVLDEGVASANPGLVDQITGYFAAQDARLHLTRSPMLVDGGEQVKNDLVML
                     NAIHQEIDQGGIDRHSYVVAIGGGAVLDMVGFAAATAHRGIRLIRVPTTILSQNDSGV
                     GVKNSVNAFGKKNFLGTFAPPYAVINDASLIESLPDREWLGGISEAIKVALLKDPEFF
                     SFIEDHALALVARDGDAMSWLIYRCAELHLQHIATSGDPFEFGSSRPLDFGHWSAHKL
                     EQLSDYRWSHGEAVAIGICLDSTYACLQGMLTEPEWRRIIDLFKAVGLPVYAPEMIAS
                     LEHQETSQSILSGLEEFREHLGGELTIMLLRGIGDGYEVHDMDESVLIECMHLLEAEH
                     AREPLSHAAVADRQPAITAGSD"
     gene            6049..6345
                     /locus_tag="VGR29_09870"
     CDS             6049..6345
                     /locus_tag="VGR29_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073936.1"
                     /translation="MQQSPIVSPLSQQDLIDEYFMEQRARIMDVAAFLDRLDRAEEQN
                     GNDDFRLVAMRKAMHELVGNEPGRVERVQMIFSDQNTDLLDELDRKAAYGASVR"
     gene            6368..7198
                     /locus_tag="VGR29_09875"
     CDS             6368..7198
                     /locus_tag="VGR29_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV2073937.1"
                     /translation="MEYIDLHAHMVSRTTDDYERMALSGCVALTEPAFWAGYDRRSAD
                     VFADYFTHLTEFEPTRAAQYGIQHFCWLCLNPKEAEDRELTRQVLDIIPEFLDRPTVL
                     GIGEIGLNRVTRNELESFKDHVELALEHNQLIHIHTPHLEDKYKGTKVIVETLAANGR
                     IDPGRVMIDHAEEHTLPMITENGFWTGFTLYPVTKTSPGRAVDMFERYGTERICVASA
                     CDWGPSDPTAIPRFALEMRRRGHNDDLIREIVFENPKRFLGQSPKFHLKRGVDARVAV
                     "
     gene            7364..>8234
                     /gene="eboE"
                     /locus_tag="VGR29_09880"
     CDS             7364..>8234
                     /gene="eboE"
                     /locus_tag="VGR29_09880"
                     /inference="COORDINATES: protein motif:HMM:NF035939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HEV2073938.1"
                     /translation="MDEHSRHLTYCMNVHPGLTLDDVITNLKTWLPPLKERFSPDQPF
                     GVGLRLAGEASREVIEDDRLRELSSVLEEHGLYVFTMNGFPYGPFHGEAVKGNVHAPD
                     WQDEERIAYTIRLIDILAALLPDDVADGSISTSPLTYKPWIDPDLPALWAVFVTNIVR
                     IAEHLVRVHQETGKLLHIDLEPEPDGLLGDGAELIRFFEEWLLPNGAPLLAERIGTDH
                     STARDLLLQHIRVCFDTCHVGVSYEQPRELFDRFDEIGIKVGKVQISSALKVSLPQDV
                     AARATIAKELEAFD"
BASE COUNT         1662 a         2439 c         2463 g         1670 t
ORIGIN      
        1 cctcctgctc gcctacaccc ttctccgccc ggtgaaactc ttctaccccg atacgctcga
       61 atcgcacctg atcgcccata cgaaggacga agggatcatc cttctccggg cgaaacaacc
      121 gtaggggtgt tcgcccgatg agattccagc cgccgggtgt cgagagggca tacgtccccg
      181 tctgctcgcc cccgatgcca atcgagccgg ccggcacatg cgtgcggggg cgtgctctcc
      241 gcggcgtggc aagctcgggg gcaagaccga tcaggtaggc aaagccggga gcaaacccta
      301 gtgcgccaac ggtgtaatga cccgccagat gccgctcgat cacctcttcc ggtaggagcc
      361 cggtatgcgc ggaaacatcc tccaggtctt ccccaaaatc atccccataa acgacaggta
      421 tcgcgacagt ccggctatgg cgggcttcag gaccgtcctc agcgaggttc cacagttcag
      481 tcaggagccg ctccagcggt tcacaagata ttaccgtctg atcgtagatc acgagaatgg
      541 tggagtagga ggggatgaca tcgaggactg atggcaccga ctccttgtca agcaactcca
      601 caaagcgtgc gatccggtag gtaaggttga catcgatgcg attgcccaca gtcatcagca
      661 tggccgaggg ggagagagga aagaatgtcg gcgaggtcat cagagtgccc ggatcgtgat
      721 gccggcgtcc ataagggcct cgcgtaaggc ggaggcaatc ggcacggctc ccggcgtatc
      781 ggaatggatg cagatggtgt cggccgcgat cgtgatgtgc ccaccatcca gcgtcgggac
      841 ctcctcgccc ccgatcatcc ggaggacccg gtcggctacc gatgccggat cctccaggac
      901 ggcgcccgcc agggaacgag gcgccagtgt gccatcgctc atgtaggcac ggtcggcgaa
      961 ggcttctcgc gctacgcgaa ggccctgtgc aaccgccgcg gcctccagct cactccccgg
     1021 cagcgcatag aaaatgacgt cgggcgccag atcgagcacc gcgttgacga tggcgtcggc
     1081 aatgatgcga tcgttcgtgg cggtgttgta cagcgctccg tggggcttca catgatgcaa
     1141 cggaacgcct gccacctggc agaaggcgtg cagggcaccg atctggtaga ggaggtccgt
     1201 ccggatttcc tccggcgttg ccgggatgtt cctccgcccg aatcctcgaa ggtcgtggta
     1261 tgaggggtgc gcaccgaccg caacgcccaa cccggcaagt gcgcggaccg tctggtccat
     1321 ggtctgcgga tctccgccat ggaaaccaca ggcaacgttc gcgctggaga tcagcgggaa
     1381 gagttcgaca tcggctccga tgcgaaaggc accgtagctc tctccaacat cgctgttgat
     1441 gtccagggcg agggtatcgg tactcatgac atgtcttccc ttccttggct gtcaatcttt
     1501 tacggtcgag tcatagagac gtgtcagata catgcggcgg acaggtgtaa agggggaggc
     1561 gaggataacg gaccggcgcg tctcctggtc gcccataaat ggtagaatgg atgtaacgat
     1621 gccggaaccc cttaaaccca tcgtcctttc ccgccgccat ccggctttcc cctgcgtgac
     1681 cacatgactg acatcattga aaccaatctt ccgacctctg ttgccgtttc tcccgagtca
     1741 cgtgtcgtgc cacggcgcaa gcggctcatc ggctttcatg accttccatt tgatgagctg
     1801 aaagtgcggc tcgtcgaaat gggggctccg gcctatcgtg cccagcagat gttcgactgg
     1861 gcctatggaa agctggtcac ctcctacgac gagatggcaa acgtcccggc acccattcgc
     1921 gacagctttg gcgagacaat accgctccag acgatgaagc tggtccgcga gttggtgacc
     1981 gataacggcg atacctacaa gctgctctac cagacctttg atggtcagct ggtggagacg
     2041 gtgctgatgc tctacccgga ccgggcgacc gtctgcgtct cctgtcaggt tggctgtgct
     2101 gtgggctgtg ccttctgcgc cactggcctg ggcggcctca cccgtaacct caccgctggc
     2161 gaaattgtcc agcagacggt cgacgccacg cgccgcgtac gccaactcga tcgccagctg
     2221 accaacctcg tgatgatggg gatgggcgag ccgctgcaga actacgcgga gacgatgaag
     2281 ttcatcaaca tcatccatga tccgcacggc ctcaactttg gcgcgcgccg gatcacgatc
     2341 tcgacctcag gcattatccc gcgcatcgat gcattggccg atgagccata ccaggtcaat
     2401 ctcgcggtct cgctccacgc gccgaacaac gagcttcgct cgcagctggt gccgatcaac
     2461 gggcgctacc cggtcgagga cctgctggcc gcctgcgatc gcttcacgca gaagacgggt
     2521 cgtcgtatct cgttcgagta cgcgctgatg aacggcatca acaacagcga cgagatcgcc
     2581 cgcgagctcg gccagcttct tcgcggccgg ctctgccacg tcaacgtgat cccgttcaac
     2641 ccggtcgatg tgctgaagtt cgaacgcccc aacgccgaga tgatcgagcg cttcgccgag
     2701 atcgtccgtg agtacggcat cccaacgacc gtccgctact cgcgtggtgt cgagattgcc
     2761 gcagcctgtg gccagctacg ggctcgccac cagcagatgg agaagactgc atcctagacg
     2821 cagtcttcta cggggctttc ggttaatccg gattcggtgt accttaatct ctccctcagt
     2881 ttactggtga gacgcgtcat ccgatgagtt ccagacacat tcagcacccg gactacgacc
     2941 aagccctcaa agtaggaggg cctgatccgt tgtcggtgtt gagctcgacc gcagctgtca
     3001 tgacctccgc acacgatctc aggatcgata tggtggccgt gcatgccgtt gccgatcggc
     3061 tcgccaggca ggaagtggcg ccggaatggg atgcggcgct ccattaccgg gcgacgggtc
     3121 tggatgccga tgagcgcacg gcgatgtggc tgctcgtgtt ggacgcgttg aacttttgct
     3181 tttgggggca gggagccgat ccatcagtcc gttggcgtgt cgaatggaag gacgaactca
     3241 cagacggtta catggccctg gtcgccgcac tcatgcgtgg ggttgaggag tgccaggaac
     3301 tccacagcgc gtcgtggctg gccactgtga agggtgaaga cgttgcgcat ctcctgcggc
     3361 ccgctgaggg gcatcccgag atcccgctct tcgaggcgcg cgtccacaac cttcgcgagc
     3421 tcggccgggg gcttctcgcg ctcgatagcg aaacaccggc gaccgcactc atcacattgg
     3481 caaacggatc tgctgtctcg ctggttcgag aggtggtgcg ccgcttcccc tcattcaagg
     3541 acattgcgat ctggacgcgt actgataccg ggctgcctgg tcacgaggtg cgcttctaca
     3601 agcgagcgca aatcctcgtt ggcgacctcg ccggcggcct ggccccttca ccactggggc
     3661 agtttcacga tctcgacaag ctgacagcct tcgccgacta caaggtgccc caggtgcttc
     3721 gtcaactggg cgtcctccgc tattcagatt ctctcgctgg ccgcatcacg cgtcgcgagc
     3781 atctcctgcc cggttccgcg gatgaaatcg agattcgcgc cgggacaatc tggggctgcg
     3841 agctggtgcg gcaggcactt gacgagcggg gacggaccgt gtccgcgcac gagctggact
     3901 ggctgctgtg gaccatggga cagtcgttgc ccacggaatc agagccgtac catctcacgc
     3961 cgacgatttt ctactaggta caaatgagta acgatccttc gcgactgtgc gagacagtag
     4021 ggtgcatgtt caccggtcga atgtccactt gacgctcggg ccccacgaag tttcggcaac
     4081 cgcgcagcag taaggagcca tcacagcgtt ttcgatccgg cacaagaccg ccccaactga
     4141 tccagggtga cgcaatacat tggtcgtccc actcagaatg actcttgcta tgagaaaggg
     4201 tagaagaaaa tactgtgcgg gaacagctac acgagaaccc tgcatggtgc tgggaaacgg
     4261 cgaaatgcct ctcacggggt gagactgtga caaggatctg gaatcgctcc tgagctgtgc
     4321 cagcgttcgt cttaacgtta tttattaaga tagagaaacc gttaccgtaa taggggatgt
     4381 ggatactgtg gaaaagtccc gaaaccggcg ccacggtgcg gaactatgcc ggtggacaag
     4441 cgggattccc catccacacc tccactggcg agatctttca cacctgtgac aatccccatg
     4501 cccctcccaa tgctgtcaaa cgatgcacac tgtacgtaca caaattggcc ataatacggg
     4561 ggcttttgtc cacaatcccc accacttatc cacatagaca agcaagttat ccacaaccgt
     4621 cctgcagcct gtgacaggca ggcaacatgg tgtcacagtc gtggcgtaat gcttgctcat
     4681 cacaaattca ctatcgctgg ttattcgata tgtcggtatg attgacgcat aggagctggg
     4741 tggatagggg aggaatgtgg gaatgccttc cagggcctca cacaatggtg cctgcctgac
     4801 aggataccgg atgcatcgtc gctctcaccg ctcgccttca aggacggata tagatgggtt
     4861 ctcttccagt tcgcgcaatt gaccagcata ttgaggttca ttattcatat caggtctgct
     4921 tcaccaggga tatcttcgat ctaaagaacg acatagttcg cgacatttgc agcggagaag
     4981 atcaggatcg acaacgcaag gttctcgtgg tgttggacga gggtgtcgct agcgctaatc
     5041 ccgggctggt cgaccagatt accgggtatt tcgctgctca ggatgccagg ctgcatctga
     5101 cacgctcgcc aatgctggtc gacggcggtg agcaggtgaa aaacgacctg gtaatgctga
     5161 atgcgatcca tcaggagatc gaccagggtg gcatcgatcg ccactcctat gtcgtggcca
     5221 tcggtggtgg tgcggtgctc gatatggtcg gcttcgccgc cgccaccgcc catcggggga
     5281 tccggctgat cagggtcccc accaccatcc tgtcccagaa tgactccggg gtcggcgtca
     5341 agaacagcgt gaacgcattc ggcaagaaga acttcttggg aacatttgca ccgccttatg
     5401 ccgttatcaa tgatgcctcg ctgatcgaga gccttccgga cagggagtgg ctcgggggca
     5461 tctcagaggc aatcaaggtc gctttgctga aggacccgga gttcttttcg ttcattgagg
     5521 accatgcgct ggcgctcgtc gcgcgcgatg gggacgcgat gagctggctc atctatcgat
     5581 gtgccgagct tcaccttcag cacatcgcga ccagcggaga tccctttgag tttgggtcat
     5641 cccgtccact cgattttggt cactggtcgg ctcacaagct ggagcaactc tctgattacc
     5701 gttggagtca tggtgaggct gtcgcgatcg ggatctgtct cgattcgacc tatgcctgtc
     5761 ttcagggaat gctgaccgag cccgagtgga ggcgcatcat cgacctcttc aaggcggtcg
     5821 gtctgcctgt ttatgcgccg gagatgatcg cctcactgga gcatcaggag acatcccaat
     5881 cgatcctgag cgggctcgag gagtttcggg agcatctggg cggtgagttg accattatgt
     5941 tgttacgcgg aatcggagat gggtacgaag tccacgatat ggatgagtcc gtcctcatag
     6001 aatgcatgca tctgctggag gcggagcatg ccagggagcc attgagtcat gcagcagtcg
     6061 ccgatcgtca gcccgctatc acagcaggat ctgattgacg aatattttat ggagcagcgc
     6121 gcccggatca tggatgttgc cgcgttcctg gatcgcctgg accgcgccga agagcagaat
     6181 gggaacgacg atttccgcct ggtcgcgatg cgcaaggcaa tgcacgagtt ggtcggcaat
     6241 gagcccggtc gtgtcgagcg ggtccagatg atcttcagcg accagaacac cgatcttctc
     6301 gacgagctgg accgcaaggc cgcctacggc gcatcggtcc gttagggtag aaaggcagca
     6361 tcacactatg gaatacatcg atcttcacgc gcacatggtc tcccggacaa cagacgatta
     6421 tgagcggatg gccctttcgg ggtgcgttgc cctcaccgag ccagcgttct gggccggata
     6481 tgatcgtcga tcggccgatg tcttcgctga ttacttcact cacctgacag agttcgagcc
     6541 cacccgcgcc gcccagtacg gcattcagca cttttgctgg ttgtgcctca atcccaagga
     6601 agccgaggac cgggagctta ctcggcaggt gctggacatc atccctgagt tcctggaccg
     6661 tccgacggtg ctcggaatcg gcgagatcgg gctcaatcgc gtgacacgca acgagctcga
     6721 gtccttcaag gatcatgtcg agctcgcgct tgagcacaac cagttgatcc acatccacac
     6781 cccccatctt gaggacaagt acaagggcac gaaggtgatt gtggagacgc tggccgccaa
     6841 tggcaggatc gatcccggac gagtgatgat cgatcatgcg gaagagcaca ccctgccgat
     6901 gatcacggag aatgggttct ggactgggtt cacgctctat ccagtcacca agacctcgcc
     6961 gggacgcgcc gtggacatgt tcgagcgcta cggcaccgaa cggatctgcg tcgcatcggc
     7021 atgtgactgg ggcccgagcg atcctacggc gatcccgcgt ttcgcccttg agatgcgccg
     7081 ccgtgggcac aatgacgacc tgatccggga gatcgtcttc gagaatccga agcgattcct
     7141 tggtcagtca ccgaagttcc atttgaagcg gggtgtggac gcaagggtag ccgtctgacg
     7201 ctggcagttc ctctgcatga gggaggggca tccagataga ctcgcccctc ccattggcgc
     7261 gattgaacgt tgacgcctaa cgggatcatt ggtcccgggc gaccgatcgc ggtcggtaca
     7321 aaaccatcgg cacaaacccg aaaggaaatc tgtcttgcgg ctcgtggacg aacattcacg
     7381 ccatcttacc tattgcatga acgtccatcc gggcttgacg ctggacgatg tcatcaccaa
     7441 cctgaagacc tggttgccgc cgttgaagga gcgtttctct ccggatcagc ccttcggtgt
     7501 cggtcttcgc ctcgccggcg aggccagccg ggaggtgatc gaggatgatc gcctgcggga
     7561 gctcagctcg gtgctcgagg agcatggcct ctacgtcttc accatgaacg gatttcccta
     7621 cgggccgttc catggcgagg cggtcaaggg caatgtccac gcgccggatt ggcaggatga
     7681 ggaacgcatc gcttatacca tccggctgat cgatatcctg gccgcgctcc tgccggatga
     7741 tgtcgcggac ggtagtatct cgacgagccc gttgacctac aagccctgga tcgatccaga
     7801 tcttcctgcc ctgtgggcgg tcttcgtcac caacatcgtc cgtattgccg aacaccttgt
     7861 gagggtgcat caggagaccg gcaagctgct ccacatcgat ctcgagccag agccggatgg
     7921 gcttctaggc gacggtgccg agctgattcg cttttttgag gagtggttgt tacccaacgg
     7981 cgcgccgctg ctcgcggagc ggatcggcac ggaccattcg acggcacgcg atctcctgct
     8041 gcagcacatc cgggtttgtt tcgacacctg ccatgtcggt gtcagctacg aacaaccacg
     8101 tgagctgttc gatcgcttcg acgagattgg gatcaaggtc ggcaaggtcc agatcagctc
     8161 cgccctgaag gtttccttgc cacaggatgt cgcggcgcgt gcgacgatcg cgaaggagct
     8221 ggaagccttc gacg
//
LOCUS       DASYWM010000244         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_86118, whole genome shotgun sequence.
ACCESSION   DASYWM010000244 DASYWM010000000
VERSION     DASYWM010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86118"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VGR29_09885"
     CDS             <1..334
                     /locus_tag="VGR29_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2073939.1"
                     /translation="CPIMDRSSSDRDLVSVADASRLLKRSTEQVRRYLREGRLPGRRV
                     GGQWFIERSAVDAFADSGRKEDRFTRRLKPASRSRPLDDVIGIGAGPGSNISSGKLAY
                     RAAGFRQR"
     gene            363..818
                     /locus_tag="VGR29_09890"
     CDS             363..818
                     /locus_tag="VGR29_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV2073940.1"
                     /translation="MGTPSSSVAEELVATDARKDDAVDLVAFVDSSAIVALVDRNDAT
                     HEAAVAAYKDLLAQGYKLFTTNHVIAETVSLLSEGAGAGVARQWLRDHRLAVYHADEQ
                     DEQRARALVIGSHSERGLSYVDAISLVVMERLGIADAFAVDTHFLSETN"
     gene            839..1294
                     /locus_tag="VGR29_09895"
     CDS             839..1294
                     /locus_tag="VGR29_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073941.1"
                     /translation="MNLNTSSGARICPECGTRNSGLSLFCAECGASLSSAGSVSADDN
                     GQTTTTFTPRSGDARQQAPEGIEATGAHTTQEFRPWTSLPEASSYNAATGWEEPRGVG
                     TYGRSPPESRRGFVLGLLAAILIALVIGFFLWSTVASQGFRDAVTGLFG"
     gene            1319..2962
                     /gene="ggt"
                     /locus_tag="VGR29_09900"
     CDS             1319..2962
                     /gene="ggt"
                     /locus_tag="VGR29_09900"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEV2073942.1"
                     /translation="MLGFPTSSSSRNYARRRPYPCYAERGMVAAAHPYVVETGLAVLR
                     QGGNAVDAAVAAGLAAAVVMPEMCGLGGELFAVLSVGAEPIAVQASGKSARGASLDLM
                     REIGGDHMPYTGAHSIAVPGMVDAYFKLLENYGTMTFARVAEPAVQLARNGFPLQPLG
                     AEAIATHADLLARDEAAADIFLANGPTPSAGDRLVQTDLAETLEQLGRQGAESFYQGD
                     LAERMTRYLQHAGGCLSVEDFAHFRTVIDAPYATIYRGHTVYQTRLPSQGVILLETLN
                     IVENAELGDLQSADGIHTLVEAKKLAYADRLGHLADPDFHQAPVEPLLSKEWAKQRFD
                     RIDLSQAATDVLYGEMHDGDTTYLCVIDSEGRMVSLIQSVSSSFGSGVVAGDTGVLMN
                     NRVGRGFSLVEGHPNIYAPGKKTMHTLNCFLIADGSGRPVLVGGTPGGDGQPQWNLQL
                     IAGMMDGGMDVQETIEQPRWTSWPGTDPSVIDNPFELRVENRLPDDVISTLEKRGHAV
                     RLQDAWGGGGAAQIIARNPDTGLLIGGTDARVEGSVLGL"
     gene            complement(3063..>3551)
                     /locus_tag="VGR29_09905"
     CDS             complement(3063..>3551)
                     /locus_tag="VGR29_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073943.1"
                     /translation="VPRLKEAAELLVKLGGNFTTDTPLAEVVAAAHEGGGICIVAHPG
                     RADLGPALTEEMLDQMLAEVPVDGIECHYRTYTDKETAYYRRLVETRGLLIGTGSDSH
                     GPGVPVDPRPFHASWSADLLGRLGLEVDSPEGPVWEAGMDPNAVPEPEPESAQEQVPA
                     QG"
BASE COUNT          677 a         1114 c         1086 g          674 t
ORIGIN      
        1 gtgtccgatt atggatcgta gcagttccga cagggatctc gtgagcgtgg cggatgcctc
       61 ccgcttgttg aaacgctcca ccgagcaggt gcgccgctac cttcgggaag ggcgtctccc
      121 tggccgccgc gtgggaggcc aatggttcat cgaacgatcg gctgtggatg ctttcgccga
      181 cagtggtcgg aaagaggatc gtttcaccag gcggctcaaa cccgcctccc gctcgcgacc
      241 gctcgacgat gtgatcggca ttggcgcagg gccgggatcg aacataagca gtggcaaact
      301 ggcataccgg gcggcaggat tccggcaacg ttagagcgga tacgcaaatc gggagtcatt
      361 tcatgggtac cccctcatct tcggtcgccg aagaactcgt cgccacggac gcaaggaagg
      421 atgatgccgt cgatctcgtc gcattcgtcg actcttcggc gatcgtcgcg ctggttgatc
      481 gcaatgacgc gacgcatgag gcagccgttg ccgcctacaa ggatctcctt gcgcagggat
      541 acaaactctt caccaccaac cacgtgatag ctgaaacggt gagcctgctt tccgaggggg
      601 cgggtgctgg cgtcgcccgg caatggctcc gtgaccatcg gcttgctgtc tatcacgccg
      661 atgagcaaga cgagcagcgg gctcgtgccc tggtgatcgg atcgcatagt gagcggggcc
      721 tttcgtatgt cgacgcgatc agcctggtgg tgatggaacg cctgggcatc gcagatgcct
      781 tcgccgtcga cacgcacttc ctcagcgaga caaactgacg cagccggagc agcactccat
      841 gaatttgaac acctcctccg gagcgcgtat ttgtcccgaa tgcggcaccc gcaacagtgg
      901 gctcagcctc ttctgcgcag agtgcggcgc aagcttgagt agcgccggaa gcgtgagcgc
      961 agacgacaac gggcagacca cgacaacctt caccccaaga agcggggatg caaggcagca
     1021 ggcgcccgaa gggattgagg cgacgggagc tcacaccaca caggagtttc gaccctggac
     1081 ctcgctgcct gaagcgtcct cttacaatgc tgccacgggg tgggaagagc cgcgaggcgt
     1141 cggaacatac ggacgctcac cgccagaaag ccgtcgaggg tttgtccttg ggcttcttgc
     1201 tgctatcttg atcgctcttg tgatcggctt tttcctctgg tccacggtgg cgagtcaagg
     1261 ctttcgcgac gcagtcaccg gtctgttcgg ctaatcttcc cgtcaagggg gtcccgtcat
     1321 gctcggattc ccgacctcat cgtcctcccg aaactacgct cgcaggcgcc cctacccctg
     1381 ctacgccgaa cggggaatgg ttgccgctgc tcacccgtac gtcgtcgaga cgggactggc
     1441 cgtgctccgc caggggggca atgctgtcga tgccgcagtc gccgccggct tggccgcggc
     1501 tgtcgtcatg ccggagatgt gcggccttgg aggtgagctc tttgccgtcc tgagcgtggg
     1561 tgccgaaccc attgctgtcc aggccagcgg caagtccgcc cggggcgcct cgctcgacct
     1621 tatgcgggag atcggcggtg accatatgcc ctataccggg gcccactcga tcgccgtacc
     1681 cggcatggtc gacgcctatt tcaaactcct cgagaactat ggcaccatga ccttcgcacg
     1741 ggttgcggag ccggccgtcc aactggcccg caatgggttt ccgctccagc cacttggagc
     1801 cgaagcgatt gccacacatg ctgacctact tgctcgcgat gaagcagcgg cggatatttt
     1861 tctggcaaac gggcctactc catcggccgg ggaccgactg gtccaaactg acctcgcgga
     1921 aacgctggag cagcttggtc gtcaaggtgc ggagtctttc tatcaaggtg acctggcgga
     1981 acgcatgacc cgctatctac agcatgcggg cggctgctta tccgtcgaag actttgcgca
     2041 cttcaggacg gtcatcgacg ctccctatgc cacaatctat cgcggccata ccgtttatca
     2101 aacccgtctt ccgtctcaag gggtgatctt gctcgagaca ttgaacatcg ttgagaacgc
     2161 cgagctgggc gatctgcaat cggcagacgg catccatacg cttgtcgagg cgaagaaact
     2221 ggcctacgcc gaccggctcg gccatctcgc cgaccccgac ttccaccagg cacccgtcga
     2281 gccactgctg agcaaggaat gggcgaaaca gcgcttcgac cggattgatc tgtcacaggc
     2341 agcgacggat gtactctacg gtgagatgca cgacggcgac acgacctacc tctgcgtcat
     2401 cgacagtgaa ggccgcatgg tttcacttat ccagtccgtc tccagctcgt tcggctccgg
     2461 tgttgttgca ggcgatacag gagtattgat gaataaccgg gtcggccggg gcttctcact
     2521 cgtcgagggg catccgaaca tctatgcgcc cggcaagaaa acgatgcaca cgctcaactg
     2581 cttcctgatc gcggacggtt ctggcagacc ggtgctcgtt ggcggcacac cgggaggcga
     2641 tgggcaacca caatggaatc tccaactcat cgccggtatg atggatggtg gcatggatgt
     2701 tcaggagacg atcgagcagc cgcgctggac gagctggcca gggactgacc cgagcgtcat
     2761 cgacaatccc ttcgagcttc gagttgagaa tcgcctgccg gacgacgtca tctccaccct
     2821 cgaaaagcgg gggcacgctg ttcgtctcca ggatgcctgg ggcggtggcg gcgcggcaca
     2881 gatcattgcc cgaaacccgg ataccggcct tctcatcggt ggcaccgatg cacgtgtcga
     2941 gggcagcgtt ctcgggcttt gacgcatcag gatcacgctc tatgtgatgc gcaaccccgg
     3001 cacggcgggt gtagacggga gaacggggct cccataggga agatgtgaag gtccagccat
     3061 aattagccct gtgctggaac ctgttcctgg gccgactctg gctccggttc cggcaccgca
     3121 ttcggatcca tgccggcttc ccacactggt ccctctggcg agtccacctc aagcccgagc
     3181 cgcccgagga gatcagccga ccaggatgca tggaagggac gaggatctac cggcacgccg
     3241 ggcccgtggg aatccgatcc tgtcccgatc agcaggcccc gcgtctcgac gagacgccga
     3301 taataagcgg tctccttgtc ggtgtaggtg cggtagtggc actcgattcc gtcaaccggc
     3361 acctcggcca gcatctggtc cagcatctct tccgtcagtg cgggaccgag atccgcacgc
     3421 ccgggatgag ccacgataca gatgccgcca ccctcgtgtg cagccgcaac cacctcagcg
     3481 agaggcgtgt ccgtcgtgaa attgcccccc agcttgacca gcaactcggc ggcctccttc
     3541 aatcgcggca c
//
LOCUS       DASYWM010000245         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_536474, whole genome shotgun sequence.
ACCESSION   DASYWM010000245 DASYWM010000000
VERSION     DASYWM010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536474"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /gene="tal"
                     /locus_tag="VGR29_09910"
     CDS             <1..1068
                     /gene="tal"
                     /locus_tag="VGR29_09910"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEV2073944.1"
                     /translation="ISRQMIQDGELQRSIEEIGIRGVTSNPTIFQKAIAAGDVYDEEI
                     TNLLKQGLQPAEVFQTVAVKDIQDACDIFRPLYDETGGEDGYVSIEVLPSLARDAEGT
                     MENARVLWSAIERPNLMVKVPGTDEGVSAIRTLLTEGININITLLFSLANYERVARAY
                     IEALARRHENGLHIESLASVASFFVSRVDTLADKLLDEKLSSGNGDPELIRSLKGKVA
                     VANAVMAYEKFEELFDSDEFKPLADAGAMVQRPLWASTGTKNPEYSDVLYVDSLIGPH
                     TVNTAPPKTIAAFLDHGTVKRTVDSDYAAAHEVLDDLHKVGLDIDEITKQLEEEGIET
                     FMSSYDELLAGVEGKRSLIEA"
     gene            1198..2646
                     /gene="gndA"
                     /locus_tag="VGR29_09915"
     CDS             1198..2646
                     /gene="gndA"
                     /locus_tag="VGR29_09915"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV2073945.1"
                     /translation="MSTVESGERHVGVVGLAVMGENLALNIERNGFAPAVYNRTAART
                     EEFLAKRAVGTDILGTFTIEEFVGALERPRRILLMVKAGSPVDAVIAELAPYLEPGDI
                     LIDGGNSLFTDTERRSREVADKGFHFIGMGVSGGEEGALWGPSLMPGGPKEAWVELEP
                     MLTAIAAKSASGPCVTHIGPGGSGHYVKMIHNGIEYGDMQLIAETYDVMRRSLGMTAA
                     EIGEEFDRWNEGKLESFLVEITGKVLKYVDPLTNEPLVDVIADQAAQKGTGRWTSQNS
                     YELGTPIPVIDAAVIARSLSSFKSRRLEASKVIKGPGSEGMPFPPIPDKQQLIDALED
                     ALYVAKITSYAQGMALLATASETYDWDLNLSEIARIWTAGCSIRAELLNPIMQAFKTN
                     PHLSNLMLAPYFVEQVNSGSPQLREVIRTAIGFGIPSSAMNAAISYIDTYRQPLLPAN
                     LIQGLRDYFGAHTYERTDREGIFHTEWMQVGV"
BASE COUNT          573 a          829 c          834 g          504 t
ORIGIN      
        1 atcagccgcc agatgatcca ggatggcgag ctccagagga gcattgagga gatcgggatt
       61 cgcggcgtca cctcgaaccc cacaatcttc cagaaagcaa tcgctgctgg tgatgtctac
      121 gacgaggaga tcacgaacct cctcaagcag ggcctgcagc cggcggaagt cttccagaca
      181 gtcgcggtga aggacatcca ggacgcctgc gacatcttcc gcccgctcta tgacgaaacc
      241 ggcggagagg atgggtatgt atctatcgaa gtgctaccga gccttgcccg cgatgccgaa
      301 ggcacaatgg agaatgcccg ggtcctctgg agcgcgatcg agcggcccaa tctcatggtg
      361 aaggtgccgg gcacggatga aggcgtttcg gcgatccgta ccttgctgac cgagggcatc
      421 aacatcaaca tcactctcct cttctcgctc gcaaactacg agcgcgtggc tcgcgcctat
      481 atcgaggctt tggccaggcg tcatgagaac ggtcttcata tcgagagtct tgcctccgtc
      541 gcgtccttct tcgtcagccg cgtcgacacg cttgcagaca agttgctgga cgagaagctg
      601 tcctcaggta acggcgaccc tgagttgatc cgaagcctga agggcaaggt tgccgtggca
      661 aacgccgtca tggcatacga gaagttcgag gagctcttcg actccgatga gttcaagcca
      721 ctggcggatg ccggcgccat ggtgcaacgg ccgctgtggg cttccaccgg caccaagaac
      781 ccggagtaca gcgacgtcct gtacgtcgat tccctgatcg gcccgcacac cgtcaatact
      841 gcgccgccga agaccatcgc ggccttcctg gatcacggga cggtgaagcg cacggtcgat
      901 tccgactatg ccgcggcgca cgaggtgctc gatgaccttc acaaggtcgg cctcgacatc
      961 gacgagatta ccaagcagct cgaggaggag ggtatcgaga ccttcatgtc ctcctatgac
     1021 gaactccttg cgggcgttga agggaaacga agcctgatcg aggcataggg caggaccggg
     1081 taccggcagg atcgccggtg cccggtgttc gtcgggagcg ttccggagct cccccagtct
     1141 ctccggtcca gtcgatccgc gggcatcaac acagcagaca gcgagggcaa gggagcaatg
     1201 agcacagtgg aatctggcga acgccatgtc ggcgtggtgg gtttggccgt catgggcgaa
     1261 aacctcgcac tcaacatcga gcgcaacgga ttcgctccag cggtctacaa ccggaccgcg
     1321 gcacgtaccg aggagttcct cgccaagcgt gccgtgggga ccgacatcct cggcacattc
     1381 accattgagg agttcgtcgg cgcgcttgag cgtcctcgtc ggatcctgct catggtgaag
     1441 gcggggtcgc cggtggatgc cgtcatcgcc gagcttgccc cgtacctcga acctggtgac
     1501 atcctgatcg acggcggcaa ctcgctcttt accgataccg aacggcgctc ccgtgaggta
     1561 gctgacaagg gcttccattt cattggcatg ggggtctccg gtggtgagga aggggccctc
     1621 tggggcccga gcctgatgcc cggtggaccg aaggaagcct gggttgaact cgagccgatg
     1681 ctcactgcca tcgcggcgaa atccgcaagc gggccgtgtg tcacccacat cgggccaggt
     1741 ggctccggcc actacgtgaa gatgatccac aacggcatcg aatacggcga catgcagttg
     1801 atcgccgaga cgtacgatgt gatgcgccgc tcgttgggga tgacggccgc tgaaatcggt
     1861 gaggagttcg accgctggaa cgagggcaaa ctggaatcct tcctcgtgga gatcaccggc
     1921 aaggtgctca agtacgttga cccgctcacg aacgagccgc tggtggatgt catcgccgac
     1981 caggctgccc aaaagggcac cggccggtgg acgagccaga actcctatga acttggcact
     2041 cccatccctg ttatcgacgc cgctgtcatc gcccgcagtc tctcgtcgtt caagagccgc
     2101 cggctggaag caagcaaggt catcaagggt cccgggtcgg agggcatgcc ttttccgcct
     2161 ataccggaca agcaacaact catcgacgca ctggaagacg cactctacgt agcgaagatc
     2221 acatcgtatg cccagggcat ggcgttgctg gccacggcat cggagaccta tgactgggat
     2281 ctaaacctct cggagatcgc aaggatctgg acggcaggct gcagcatccg tgccgagctg
     2341 ctcaatccca tcatgcaggc gttcaagacg aacccacatc tctcgaatct gatgctggcg
     2401 ccctattttg ttgaacaggt gaactccggc agtccacaat tacgtgaggt gatccgcaca
     2461 gcgatcggct tcggcatccc ctcctcggca atgaatgcgg caatcagcta tatcgacaca
     2521 taccggcaac ctttgctgcc cgccaacctg attcagggtc tccgcgatta cttcggcgca
     2581 cacacgtacg agcgcaccga ccgtgaaggc atcttccata cagaatggat gcaagtcgga
     2641 gtctgaagcg gcggcacatt gttcgcgctg catggatgtc ggccgggagg ccgttgatgt
     2701 tggccactcg ggccaatcca gtcacgccag gcgggtgact
//
LOCUS       DASYWM010000246        21810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_593276, whole genome shotgun sequence.
ACCESSION   DASYWM010000246 DASYWM010000000
VERSION     DASYWM010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 21810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21810
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593276"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..950
                     /locus_tag="VGR29_09920"
     CDS             195..950
                     /locus_tag="VGR29_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2073946.1"
                     /translation="MSWQVRSGEHWVVLGPNGAGKTTMLSVVAAERHPSEGDVAILGE
                     RFGRTDMRELRRRIGRVDPALRMLDWLTGREAVLTGLRNTIWPRWEDWNDPEYARARE
                     ALALVGSTEFAEREIATLSHGERQRVRIARALIQEPELLLLDEPATGLDFPAREALLS
                     SLDGLAAQRPYLPTVMVSHHLEDLPMSVTHALLLRQGEVLASGDVREMLTSELVTECY
                     GFPIEVHEFDGRWAARAAAGWTVQDRAEREVAQ"
     gene            947..2236
                     /locus_tag="VGR29_09925"
     CDS             947..2236
                     /locus_tag="VGR29_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEV2073947.1"
                     /translation="MSDDLHQYDVVIAGGHVIDPASGLSDQRDIAIHDGVIAAVESNI
                     AAGSARRMIDARGQIVTPGLVDLHTHIYWGATYWGVEADPIAARSGVTTWIDVGSAGG
                     YSFPGFREYIALRSSVRVYCFLHLSSIGLVAPTWELSNPDYWDVDLAAKTVEQNRDII
                     LGIKIRMDSRTTRGVGIEPMKRARELADRVDLPLMTHIGDGPPSLEDLLAYLRPGDIL
                     THCFTGRSMGICGPDGHVLPEIRSLQEQGLILDIGHGTGAFSFDVAEKMLADGILPDA
                     ISTDIHQTAVQGPAFDMPTTMTKFLMLAMPLEEIVKRTTVNPARAVHLEGAGSLAVGG
                     RADIALFRIEDGSYAYYDVFMNERRTNRRIVSTLTLVNGHELPRTEERPVHFFAELPE
                     AQRPILNVEGSGHLVPEAKAVNQDEFRRDVHEVQGGL"
     gene            2236..2886
                     /locus_tag="VGR29_09930"
     CDS             2236..2886
                     /locus_tag="VGR29_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEV2073948.1"
                     /translation="MLRDHIEGNPDINYGWYLRPSYAMSRAQAEMHDLLRRQFGLIGG
                     GAFMPHATIKGFFRSDATIPELIAAFDTAVEGHEEFVVYNKGPVPYGRTSVVLDINET
                     PDGSVNAPLLALHQSAWNAIAPLVHPDCPVTPREPAMDRFHAHLTLAMADLREDFFDE
                     VVAFISEAAPIGPKSFTAQFCHLFAFRSKNWAGDWWNTLEWQMLHSWKLGSRTKDA"
     gene            2888..3817
                     /gene="murI"
                     /locus_tag="VGR29_09935"
     CDS             2888..3817
                     /gene="murI"
                     /locus_tag="VGR29_09935"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV2073949.1"
                     /translation="MHEGTQPIGMFDSGLGGLSVYREVRKYLPAEDVIYYADSAYCPY
                     GTRSPEEIRQRCHMISRLLIEQGSKIIVVACNTASSMAIDFLRFSFPHISFVGLEPAV
                     KPAAEITRTGKVGVLATPRTVAGDRLRWLIETHASGVEVRTTPGNGLVELVEAGTLRG
                     EMVRETFGPMLAPMLEAGVDVVVLGCTHYPFLRDEIQDYVGPEVEIIDSGAAIARRII
                     NVLRAQNLLGESNQQGSFRLLTSAPAEETGRVASLLLGMPISAEEMRCQSHAERKEAV
                     SLPGPPGLTTSGRDCPEASPSVVEPVPASAVRQ"
     gene            complement(3838..4644)
                     /locus_tag="VGR29_09940"
     CDS             complement(3838..4644)
                     /locus_tag="VGR29_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073950.1"
                     /translation="MNSTLQVIVDYYSAHPEVTAIALGGSHASRQADERSDNDVYLFT
                     TAPVGHQTRRDLALRFDESPEIGNSWWGDDDAWSDGTAGYEVMYWDSADFEQGLRRVI
                     EEHQPSLGYSTSFWFTMRNALPLYDRDDWLSRMKSLAETPYPEELRRAIVDFNAPLLR
                     RIHASYRHQIALAVGRNDPVSVNHRIAALLASVFDIVFALSRTLHPGEKRQLAWIATL
                     GEQVPATLDRRVRGLLAAACDTAGHEIMPAVDALCGDIEGMIVEAGFPAR"
     gene            complement(4886..7342)
                     /gene="lon"
                     /locus_tag="VGR29_09945"
     CDS             complement(4886..7342)
                     /gene="lon"
                     /locus_tag="VGR29_09945"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV2073951.1"
                     /translation="MRQHRVADPRAEHDTTDEVPLLPFRNIVVFPRNVVTLQVGRPRS
                     IHAVEEAMITGRMIAITAHRDQDIDDPRPGDLFDVATLGSILTVDRQPGGNLQVVLEG
                     IGRIRLLDIQNKRPFFMATVEEIEEPPVDADEAAAMVRHIQELTLRYSETNSELSTEV
                     LDMVQRASNPGHLADLLATQVIKEVPRRQELLEIADPLHRLERLGALFASELEIAELD
                     RKIKDRIHKEMDDTQRQYYLTKQLEIIQNELGGDESNEYQQLRTKIQESGIPAYAQEK
                     LQRELTRLERMPSVSAESTVVRTYIETMLALPWGEQTEDRIDLGEAEEILDQDHYGLE
                     EVKERIVEFLAVRSLTQEHGTFTAAQILCLAGPPGVGKTSLGRSIAEAMGRKFVRVSL
                     GGVRDEAEIRGHRRTYIGAMPGRIINAMKTAESMNPVILLDEIDKLASDYRGDPTAAM
                     LEVLDPEQNSHFTDHYLDMPYNLSQVLFITTANYVNQIPRPLRDRMEMLEVTGYTEDE
                     KIEIARRHLLRRQLEASGLPESAVEIAPPIWRQLIRDYTREAGVRGLDREVARLCRKI
                     ATLIVRGKTDRDGKITITRERLEEFLGPKKYGFEQDLGGSQVGLAIGLGTTEIGGEII
                     PVEVATMPGRGNLTITGQAGDVMQESARAALSYARSRAEELKIDKDFQEKLDLHIHLA
                     EGATPKDGPSAGITMATALISALTQRPIRNDTAMTGEITLRGRVLGIGGLRDKALAAH
                     RHGLKRLIAPIDNQRDMREIPQNVREEMEFIFASSMDDVIQAAIMLDVEQADHLQTMQ
                     DRVFTTVPKIQPDAVDALGR"
     gene            complement(7604..8422)
                     /locus_tag="VGR29_09950"
     CDS             complement(7604..8422)
                     /locus_tag="VGR29_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEV2073952.1"
                     /translation="MPSRNDHNPGQPVDVPIQPERRDRAHGRAIADQNLLSWSRDDRE
                     KAAAFTHSDPWRVLRIMGEFVAGFDALAEIGPGVTIFGSARIAEGDPFYEQARELGRR
                     LASAGVTVITGGGPGIMEATNRGAFEADGESVGAGIELPFEMGLNSYVNVGMEFRYFF
                     VRKVMFVKYASGFVFFPGGFGTLDELFEVLTLIQTGRLDRVPVVLFGSNHWRGLLSWF
                     DDVLERDGRISPNDKSIFTITDNIEETARVLIEATLRSNGIHDAEPTSIPALPR"
     gene            8603..9121
                     /locus_tag="VGR29_09955"
     CDS             8603..9121
                     /locus_tag="VGR29_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073953.1"
                     /translation="MTTDWGSEEKGRCLVLALMAASVLVLAACGGSDDDGAGQERTIA
                     AIDAVATPPAAAELLGVTWTTAVDPETRVPKNEVTSFPNDAPAIIAAVEVGEVQAGTE
                     LTATWSIDGVEVPKASMRVTAQQDLQEGWATFQFTRDPARLFPLGELEVRVTAPGDDA
                     VTATVDIVLPEN"
     gene            complement(9312..9851)
                     /locus_tag="VGR29_09960"
     CDS             complement(9312..9851)
                     /locus_tag="VGR29_09960"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEV2073954.1"
                     /translation="MSTDERRLSGFRERKDTFFREQEQSPLTQEQKVAFTGLSYFPTN
                     PDLSLILELDKSGDGIGEEITVGTMTGEPKQYIRIGRIRFEVDGEEATLSVFQDAKTG
                     KLFLPFRDTTADDETYSVGRYLDPKVRPDGRLVVDFNMAYNPHCAYNSGWSCPIPPFE
                     NRLKVPIRAGELIPNIPHE"
     gene            complement(9965..10567)
                     /locus_tag="VGR29_09965"
     CDS             complement(9965..10567)
                     /locus_tag="VGR29_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEV2073955.1"
                     /translation="MNEKTTATASDTVANVHAEAQEVAEETREETVLAFIEIPRGSRN
                     KYEFDEESGTFFLDRVLYSSVHYPTDYGFIPDTLADDGDHLDVCVLMTEPSFPGCTIV
                     ARPLGGLDMRDEKGEDFKLLAVPVNDPRFAHYKSLDQVGKHWLREIETFFATYKLLEN
                     KETNILGWYGLDFARQQIEASRENYRNSRAADRIEKNEKI"
     gene            complement(10883..11770)
                     /locus_tag="VGR29_09970"
     CDS             complement(10883..11770)
                     /locus_tag="VGR29_09970"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEV2073956.1"
                     /translation="MDRFVRWATAQPAIEAAMVVGSRARTDHPADAWSDLDIVIFATD
                     PEILLENDAWLHNIGDPVITFLEATAVGAWRERRVLFDGARDVDFSVVPADLLNELNA
                     MHPGDLLHAEVGATIARGYRVLIDRNERLTPILRRITTITECEPLPPTYEQFDETIND
                     FWYHCVWIAKKLRRGDLIVAHECLDGHQRRLIMHLIRWHPERKGTLWHGARFMEEWAP
                     QEVTMRLATTWAEHDTESIRCALTNMMDLVAWLTKEIANAYSLEETPVAPEVAARRWV
                     TEISMSPPPGEVHSGPRAG"
     gene            complement(11815..12672)
                     /locus_tag="VGR29_09975"
     CDS             complement(11815..12672)
                     /locus_tag="VGR29_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV2073957.1"
                     /translation="MTRLGVITDEISEDLDTALGVCTDLGIRDIELRSIWNTSIVEHD
                     GASIRVIADTIRAREFSVCGIASPFLKCHISGDGAAAGATHSAQATTRREQWDILERS
                     LDVAALLNAPMVRAFSFWRVDDPVSVREEILGILQEATVRTRSAGRLLGLENEYSCNI
                     ATGKEAAWYLERIPDPTFGLIWDPGNEAALGHAPIPEGYEAVRERIHHIHLKDAVRPG
                     EPVEFTVIGDGIIGYEEQFRLLKRDGYEDVLSLETHFDLKGQREGATRACAHAVRSIA
                     AQAGLPVGP"
     gene            complement(12717..13331)
                     /locus_tag="VGR29_09980"
     CDS             complement(12717..13331)
                     /locus_tag="VGR29_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV2073958.1"
                     /translation="MIQSHEHSPVADPDLAARFPALARGVRWGNTALTGCHLITGKPD
                     PARLQSVNMVPFVGDRMIVIALADGHVMLPGGTRERGETLLQTISREMREETGYALES
                     CYPFATLKCISFDEKPWRDYLVHPEFERLVCFGEVHRIGVPENPHGAEQITRIDVLDV
                     NEAIAFLQDADRPELADLYRLAAQIRQTNHGLFDLAIDVGQHAP"
     gene            complement(13468..14991)
                     /gene="dnaA"
                     /locus_tag="VGR29_09985"
     CDS             complement(13468..14991)
                     /gene="dnaA"
                     /locus_tag="VGR29_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV2073959.1"
                     /translation="MSIWYAGDPGVSAPREHNPSDTTGASDASINVNQLWQTVLFDLE
                     SRISRSAFDNWFRQTSLAGVDNDIAVVHAPNAFSASTLQGRYASQVERALSDVIGRRI
                     SVEFTVNHEMADRPGRARAATTARAADSAPGSPPPKPVRRPERVADRSLTGLGNRQLV
                     LESSAHGLVPRLTFETYVVGSSNRLAHAAAQAIAERPGAQYNPFFVHGGVGLGKTHLL
                     HAVGHRALELSPDLKIMYVTSETFTNDVINAIRSQRMEDFRGRYRSIDILMVDDIQFI
                     AGKESTQEEFFHTFNALYQNGKQVIVSSDQPPRSIAALEERMRSRFAGGLIADVQSPD
                     FEMRTAILRTKAEELGVNMPPDVIEYIAHRDQTNIRELEGALNKILMMAQLYNRRLNL
                     TLAMEALTDASNTQRRAKTTASDVVDAVVRHFKVQEKDLKGRQRTRDIVFPRQVAMYL
                     LREETDISLEEIGRSMGGRDHTTVLHGIKKIESALDSDVQLRASLMAIREDLLTGQE"
     gene            15314..16528
                     /gene="mnmA"
                     /locus_tag="VGR29_09990"
     CDS             15314..16528
                     /gene="mnmA"
                     /locus_tag="VGR29_09990"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV2073960.1"
                     /translation="MSETFETTLPAADLADVADLLSEGKIGPRDGAGKTVVVAMSGGV
                     DSAVTALIMRERGYNVVGMNLRLFSPEDEDHRANPCCGIPAMDDARATCATIGVPFYA
                     INMEIEFGGAVIQKFIDEYAAGRTPNPCLECNRHVKFRHLVQRAKLLGAGCLATGHYA
                     RIERGEPASGIPHRLYRALDDRKDQSYVLYTLNQEQLDYIQFPLGGLTKPETRRLARA
                     YGLPVADKAESQEICFVGNRSYADFVAERRPDVTKPGEIITKAGEVIGQHNGLVNHTV
                     GQRRGIRVAAPKPYFVLHLDTDANQLVVGDREDAEAKALRVDGVAMASGGWPHEPFDA
                     EIVVRYRGVPSAARVQLESSGQRTAHITLLGDRGPIASPGQAVVFYRGDEVLGGGTIC
                     DVERRGNEELSA"
     gene            16646..18415
                     /locus_tag="VGR29_09995"
     CDS             16646..18415
                     /locus_tag="VGR29_09995"
                     /inference="COORDINATES: protein motif:HMM:NF008183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV2073961.1"
                     /translation="MSGRSESPGQSTVSHDVDVWWKHAVVYQIYPRSFTDSNGDGIGD
                     VPGIIEKLDYLAELGVDVVWLSPIYRSPQDDNGYDISDYQDIDPMFGTLEDVDRLLQE
                     AHRRNIKILMDLVVNHTSDEHPWFIESRSSIDNPKRDWYWWRPAHEGMTAGAPGAEPS
                     NWRSLFSGSAWELDETTGEYYLHLFSRRQPDLNWENPDVREAIYEMMNWWLDRGIDGF
                     RMDVINMISKETSLPDGHVTGPGFYGDGSPFFMNGPRSHEFLQEMHREVFAGREARLL
                     TVGEMPGATVDEALLYTDPERNELDMIFQFEHMELDHVTSRYDTKPLNLTDLKASLGR
                     WQVGLADVGWNSLYWNNHDQPRIVSRFGDDGEYRVESAKMLGTVLHMHRGTPYVYQGE
                     EIGMTNMSFTSRADFQDIATINFYQEALKHGTVTDDLFEKLAAGTRDNARTPMQWDSS
                     EKGGFTTGVPWFPVNPNTSEINVDAAMADEHSVFHHYRKLIALRHDLPVVALGNFNML
                     LEWDPHIYAFTRSLNGVELLVLGNFSAKELPVDLPDGDAWAGAELLLGNYNEVSADAP
                     SIVLQPWEARIYRREQGETGNAS"
     gene            18542..18967
                     /locus_tag="VGR29_10000"
     CDS             18542..18967
                     /locus_tag="VGR29_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073962.1"
                     /translation="MKQKAIIGSVVAAVVFIAIIVGLYLLGGEDQSALERLRDIAIIF
                     IVLLSLVTVILLAGITAALAFLAFQIKDRVIPLLEEVTGTVNRVRNTTNYMTEEAVKP
                     LISVASNFSKIRQMSKTVTGKRKRPPKPPTYRSAEEASR"
     gene            18980..19375
                     /locus_tag="VGR29_10005"
     CDS             18980..19375
                     /locus_tag="VGR29_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV2073963.1"
                     /translation="MEEFLKNARKQLEELDLQGQAKDIDLGKLKQLTLREQQQREEEA
                     ASEGFLGGMLLGIVVGALLALIFAPKSGSETREMVAGTASELKHKAEGLVGQSKNGST
                     DQAASTLSDEPAIEREIGDTSTRSTPGVI"
     gene            complement(20437..21246)
                     /locus_tag="VGR29_10010"
     CDS             complement(20437..21246)
                     /locus_tag="VGR29_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEV2073964.1"
                     /translation="MPDSNDNCAAYNPGQEDTDSDGTADACDSTPTGDTDGGGVDNAV
                     DLTPNGDDDGDGVDNNLDVCAAGDDGVDTDTDDIPDACDSTPTGDTDGDGVADTSDNC
                     PTTANPGQEDTDSDGTGDACDSVDNGDTDLDGIQNYADNCRSVANSDQADTDGDGIGN
                     ACDSMPNGVVVPSITYDVTQWYINPVTKERECGVHVTVREVYQPYRVILTYSNGEIVD
                     YPVTEYFVLQDGLFEWTFGGGAALPYGETVVSVRATGGGVNLSAMFEQTCR"
     gene            complement(21220..21600)
                     /locus_tag="VGR29_10015"
                     /pseudo
     CDS             complement(21220..21600)
                     /locus_tag="VGR29_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4243 a         6336 c         6798 g         4433 t
ORIGIN      
        1 acataactgc ggggccggtg tcaatgtgat tccatggagg aattgcatac agtggtcaat
       61 gcgtcaattg atatggttcc ataaccggag agagttgctc ctgtcatttg accgaaaggg
      121 cttatgtgac cgacgtcatc cagcttgtga atgtctccat ttggacaccc gaaaagaagc
      181 ggatcctaaa cgacgtgagc tggcaggtcc ggagcggcga gcattgggtt gttctcgggc
      241 cgaacggggc ggggaaaacc acgatgctgt ccgtggttgc ggcggagcgt caccccagtg
      301 agggagatgt ggctatcctc ggtgagcggt tcggcaggac ggatatgcgg gagcttcggc
      361 gacggatcgg tcgggtcgat cctgccctcc gcatgctgga ctggttgacc ggccgtgagg
      421 ccgtcctgac gggcctgagg aacacaatct ggccgcgctg ggaagactgg aacgatccgg
      481 aatatgcacg ggctcgtgag gccctggcgc tcgttggcag cactgagttc gccgaacggg
      541 agatcgccac gctctcccat ggtgagcgac aacgggtgcg cattgcccga gcattgatcc
      601 aggagccgga gctcttgctg ctcgatgagc cagcgaccgg cctggatttt ccggcccggg
      661 aggcacttct ttcgtcactt gacggactgg cggcccagcg gccttatctg ccgacggtca
      721 tggtctcgca tcacctcgag gatttgccaa tgagcgtgac gcatgcgctc cttctgcggc
      781 agggcgaagt gctggcctcg ggagatgtcc gcgagatgtt gacgagcgag ctggtgactg
      841 aatgttacgg gttccccatt gaggttcacg agttcgacgg tcgctgggca gcacgggcag
      901 cggccggctg gacggtgcag gaccgggcag aaagagaggt cgcgcagtga gtgatgatct
      961 gcaccagtac gatgttgtga tagcgggagg gcatgtaatc gatcctgcat ccggtctctc
     1021 agatcagcgg gacatcgcca ttcacgatgg cgtgatcgcc gcggtggaat cgaatatcgc
     1081 cgcaggctcc gcgagacgaa tgatcgatgc ccgcggccag atcgttacac ccggtttggt
     1141 tgacctgcac acacacatct actggggtgc aacgtactgg ggcgtcgagg ccgatcccat
     1201 cgcggcgcga tccggtgtta cgacatggat cgacgtgggc agcgcgggcg gctacagctt
     1261 ccccggcttc cgcgaataca tcgctctccg ctcctcagtg cgtgtctatt gcttccttca
     1321 cctttcctcc atcggtttgg tggcgccaac atgggaactc tccaatccag attactggga
     1381 tgtagacctc gccgccaaga cagtcgaaca aaatcgcgac attatcctgg ggatcaagat
     1441 ccgcatggat agccggacca cgcggggcgt tggtatcgaa ccgatgaagc gggcgcggga
     1501 gctggctgat cgggttgacc tgccactgat gacgcatatt ggcgatggcc cgccctcgct
     1561 ggaagatctg ctggcgtacc tgcggcccgg cgatatcctg acgcactgct ttacgggtcg
     1621 aagcatggga atttgcgggc cggatggtca cgtcctgccc gagatccgga gccttcagga
     1681 gcaggggctg atcctcgata tcgggcacgg tacgggcgca ttcagcttcg atgttgccga
     1741 gaaaatgctc gccgacggca tcctgccaga tgccatctcg accgacattc accagactgc
     1801 agtgcagggc ccggccttcg atatgccgac gacgatgaca aagtttctca tgcttgccat
     1861 gccactggag gaaatcgtta agcgaacgac cgtcaacccc gcgcgagcgg tccaccttga
     1921 aggcgcggga agcctcgcag tgggcggccg tgcagacatt gcgctcttcc ggatcgagga
     1981 tggatcgtat gcatactacg acgtcttcat gaacgagcgc aggacgaacc gtcggatcgt
     2041 gagcaccttg acgctggtca atggccacga gctcccacga acagaggagc ggccagtaca
     2101 cttctttgcg gagttgcccg aagcgcagcg gcctatcctc aacgtcgaag gttcaggcca
     2161 tcttgttcct gaagccaaag ccgtgaacca ggacgagttt agacgagatg tgcatgaggt
     2221 ccagggaggg ctctgatgct ccgtgaccac atcgagggca atccggacat caactatggg
     2281 tggtaccttc gtccctcgta cgccatgagc cgggcacagg ccgagatgca cgatttactg
     2341 cgacggcagt tcggtctgat cggcggggga gcgtttatgc ctcatgccac aatcaagggg
     2401 ttcttccgat cggatgcgac cataccggaa ttgatcgcag ccttcgacac tgccgtcgag
     2461 gggcacgagg agttcgtggt ctataacaag ggccctgttc cctatggccg taccagcgtt
     2521 gtgctggaca tcaatgagac gccagatggc tcggtaaatg cacccttgct cgcgctgcat
     2581 cagtcggcgt ggaatgcaat tgctcccctg gttcatccgg attgtccggt aacccctcgc
     2641 gaaccggcga tggatcggtt tcacgctcac ctgacgcttg ccatggcgga cctgcgggag
     2701 gatttctttg atgaggtcgt tgccttcatc agcgaggctg cccccatcgg gcccaaatct
     2761 ttcaccgctc agttctgtca tctcttcgct ttccggagca agaactgggc tggtgactgg
     2821 tggaacaccc tggagtggca gatgctccac agctggaagc tcggctcccg gacgaaggat
     2881 gcctgagatg cacgagggga cgcagcccat cgggatgttc gactctggtc tgggcggatt
     2941 aagtgtctat cgcgaggtcc gcaagtatct tccggccgag gacgtgattt attacgccga
     3001 cagcgcgtat tgcccgtacg ggacgagatc cccggaggag attcgccaga gatgtcacat
     3061 gatctcccgt ctgctcatcg agcaaggtag caagatcatc gttgtcgcat gcaataccgc
     3121 cagctccatg gccatcgatt tcctgcgatt cagttttcct catatctcct tcgtcggtct
     3181 tgagcctgcg gtgaagccag ccgccgagat cacccgcacg gggaaggtcg gagtcctcgc
     3241 aacgccacgc actgtggccg gagaccggct ccgctggctg atcgagacgc acgcgagtgg
     3301 cgtcgaggtc cggacaacgc cggggaacgg gctggttgag ctcgttgagg ccgggacgtt
     3361 gcgtggcgaa atggtacgag agacgtttgg gccgatgctg gccccgatgt tggaagctgg
     3421 ggtcgatgtg gtcgtgctcg gctgtaccca ctacccattc ctgcgagatg agattcagga
     3481 ttacgttggc ccagaagtcg agatcatcga tagcggtgcc gccattgccc gccggatcat
     3541 caacgtgctt cgggcccaga acctgttggg cgagagtaat cagcaaggca gctttcgctt
     3601 gctgaccagt gcaccggcgg aggaaacggg ccgggtagct agcctgttgc ttggcatgcc
     3661 gatcagtgca gaagagatgc gttgccagag ccacgcagaa cggaaggaag ccgtttcact
     3721 cccgggcccg cctggtttga ctacttctgg ccgggattgc cccgaagctt caccatccgt
     3781 agtggagccg gttcctgctt cagcagtacg gcaataggtg gagcaccata ggaggggtca
     3841 ccgtgctgga aatcccgcct caacaatcat gccctcaatg tcgccacaca gtgcatcgac
     3901 agccggcatg atctcgtgac cagcggtatc gcaggcggcg gccagcaatc cgcggacgcg
     3961 gcggtcgagc gttgccggca cctgttcccc aagggtggcg atccaggcga gctggcgttt
     4021 ttctccagga tgcaaagtcc gggataacgc gaatacgata tcgaacacgc tggcaagcaa
     4081 cgcggcaatg cggtgattca cgctcaccgg atcgttgcgc ccgacggcaa gggcgatctg
     4141 atgacggtag gaggcatgga tgcgtcgaag aagtggtgca ttgaagtcca cgatcgcgcg
     4201 gcgtagctcc tcgggatagg gtgtctccgc cagcgacttc atccgcgata gccaatcgtc
     4261 ccggtcgtag aggggcaacg cattcctcat ggtgaaccag aacgatgtcg agtagccgag
     4321 cgacggttgg tgctcctcga tgacccgacg aagcccctgc tcgaaatctg cggaatccca
     4381 gtacatcact tcatatcctg cggtgccgtc gctccacgcg tcatcgtcac cccaccacga
     4441 attgccaatc tctggagact catcgaagcg aagagcgaga tcacgacgcg tctgatggcc
     4501 taccggcgca gtggtgaaga gatagacatc attgtcagat cgctcgtccg cttgccggga
     4561 agcgtgtgag ccgccgaggg cgatcgcggt aacctcgggg tgggcagagt agtaatcgac
     4621 aatcacctgc agcgtgctat tcatttgcgt ccttaggaaa atgcgtaagt caccatcact
     4681 atttgtaatg caattctcgc cggtatcacg gagtagggaa actcacgcgg actggtcgaa
     4741 atgccggagg actactctac tctgcctgtg gagtggtcca ggattgcagt tcggcagtgc
     4801 tgacctcaat cagcggagat cggttccagc cacagtcgaa cctcacccat aaggtgggga
     4861 ggttcgacga gacactcctg attcgtcagc ggccgagggc gtcgacggca tcgggctgga
     4921 tcttggggac ggtagtaaag acacggtcct gcatcgtctg caggtgatcg gcctgctcga
     4981 catccagcat aatcgccgcc tggatgacgt catccatgct ggaggcaaag atgaactcca
     5041 tctcctcccg gacgttttgc ggtatctccc tcatatcgcg ctgattgtcg atcggcgcga
     5101 tgagccgctt cagcccgtgg cggtgggcgg caagtgcctt gtcccgcaaa ccaccgatgc
     5161 cgagcacccg tccgcgcagg gtgatctcac cggtcattgc agtgtcgttg cggatcggtc
     5221 gctgggtaag ggcggaaatt agcgcggttg ccatggtgat tccagcggac ggaccatcct
     5281 ttggtgtggc accttctgcc aggtggatgt gcagatccag cttctcctgg aagtccttgt
     5341 cgattttgag ctcctcggcg cgggatcgtg cgtaggacag cgcggcgcgg gcggactcct
     5401 gcatgacgtc gccagcttgg ccagtgatgg tcaggttgcc gcgtccgggc attgtcgcaa
     5461 cttcaacagg aatgatctcg ccgccgattt cggtcgttcc gagcccgatc gcgaggccaa
     5521 cctgactccc ccccaggtcc tgttcgaacc cgtacttctt gggtcccagg aactcctcca
     5581 gtcgctccct ggtgatcgtg atcttgccat cgcgatccgt cttgccccgg acgatcagcg
     5641 tggcgatctt gcggcaaagg cgggcgacct cgcggtcaag gccacggaca ccggcctccc
     5701 gtgtgtagtc gcggatcaac tgtcgccaga tcggcggcgc aatctcgacc gcgctctccg
     5761 gcagcccact cgcttcaagc tggcgccgca aaagatgacg acgggcgatc tcgatctttt
     5821 cgtcttcggt gtagccggtg acctccagca tctccatgcg gtcccggagt ggccgtggga
     5881 tctggttgac gtagttggcg gtcgtgataa agagcacttg cgagaggtta tagggcatat
     5941 cgaggtagtg atccgtgaaa tgggaattct gttccggatc gagtacctcg agcatggccg
     6001 ccgtcggatc gccacgataa tcgctcgcaa gcttgtcgat ctcgtccagc aggataaccg
     6061 ggttcatcga ctcggccgtc ttcatcgcgt tgatgatgcg accgggcata gcaccgatat
     6121 aggtgcgccg gtgtccccgg atctctgctt catcccggac tccaccgagg ctgacccgga
     6181 cgaacttccg acccatcgct tcggcgatcg agcgtccgag gctcgtcttg cccacgcctg
     6241 gaggaccagc aaggcacagg atctgggcag cggtgaacgt gccatgctcc tgggtcagtg
     6301 agcgcacggc caggaattcg acgatgcgct ccttgacctc ttccagtccg taatgatcct
     6361 ggtccaggat ctcttcagcc tcgccgagat caatgcgatc ttccgtctgc tcgccccacg
     6421 gtagggcaag catcgtctcg atgtaggtac gaacgacggt ggactcggcc gagacgctcg
     6481 gcatgcgttc cagacgggtg agctcccgtt ggagcttttc ctgagcatac gcgggtatgc
     6541 cactttcctg gatcttggtc cggagctgct ggtactcatt gctttcgtca ccaccgagct
     6601 cattctggat gatctccagc tgcttggtca ggtaatactg ccgctgcgtg tcatccattt
     6661 ccttgtggat gcggtccttg atcttgcggt caagctccgc tatctccaac tcgctggcga
     6721 agagggcacc gaggcgctca aggcgatgca gcggatccgc gatttcgagc aactcctggc
     6781 gtctcggaac ctccttgatt acctgggttg ccaggaggtc tgcaaggtgg ccggggttac
     6841 tcgcgcgctg caccatatcg agcacttcag tggagagttc actgtttgtc tccgagtaac
     6901 ggagagtcag ctcctggata tgccgcacca tggccgccgc ttcgtcggcg tcaactggtg
     6961 gttcctcgat ctcctccact gtcgccatga agaaggggcg cttgttctgg atatcgagca
     7021 ggcgaatacg gccgatgcct tcaaggacaa cctgaaggtt gccgccaggc tggcggtcga
     7081 cggtgaggat actgccaagt gtcgcgacat cgaaaagatc acccggccgg gggtcgtcga
     7141 tatcctgatc gcgatgcgcg gtgatcgcaa tcatgcggcc ggtgatcatc gcttcctcga
     7201 cggcatggat tgagcgcgga cggccgactt gcagcgtcac aacatttcgc gggaagacaa
     7261 caatgttccg gaacggaagc agcggtacct cgtcggttgt atcgtgttcg gcccggggat
     7321 ccgccactcg gtgttgtcgc atgcgacatt atctccatag ggatgtgaac aggtacagga
     7381 tgttgcgctg tgaggacaca gggtcacgca gcctacatga ccaacttatc tcgcacctgc
     7441 gccgtcatag taccgcaggc ggggatgttc tgctaccgaa gttcgacccg cccgaaggcg
     7501 agccgtggac aagaaaaggc tttaaccacg cgaaatttcg atgcagatcc caccaccagg
     7561 gagtcgcttg aatcgaggtt tcagtggcca gtcgtgccga gttctaacgt ggtagggccg
     7621 ggattgatgt cggctcggcg tcatggatgc cgttggacct cagtgtcgcc tcaatcaata
     7681 cccgggccgt ctcttcaatg ttgtccgtga tcgtaaagat gctcttgtcg ttgggcgaga
     7741 tccttccgtc gcgctcaagg acgtcatcaa accaggagag cagtccgcgc caatgattgc
     7801 tgccgaagag cacgacgggt acccgatcaa gcctgccggt ctggatgagc gtgagcacct
     7861 cgaagagttc atcgagcgtg ccgaacccgc cggggaagaa gacgaagccg gaggcgtact
     7921 tcacgaacat gaccttgcgc acgaagaagt accggaactc catcccgaca ttgacgtagc
     7981 tgttcagccc catctcgaac ggaagctcga tacccgcccc tacggactct ccatcagctt
     8041 caaatgcgcc gcgattggtc gcttccatga tgcccgggcc accacctgtg atgaccgtga
     8101 caccggccga tgcgagacgc ctgccgagct cgcgtgcctg ctcgtaaaaa ggatcaccct
     8161 cggcaatccg ggccgagccg aagatggtta cgccagggcc gatctctgcg agcgcatcaa
     8221 acccggcgac aaactccccc atgatgcgca ggactctcca tgggtcgcta tgggtgaagg
     8281 cggccgcctt ctctcggtca tccctggacc acgagagcag gttctggtcg gcgatagctc
     8341 gtccgtgtgc gcgatcacgg cgctccggct ggatcggcac atctaccggt tgcccgggat
     8401 tgtgatcatt tctggaaggc atggtgagtt cctgtgaatg ccggaggcgt cctccgggaa
     8461 agtacaatcg tcgcggcaat gtgtagggcg ttacgactgg catgccagcg tggacatcgt
     8521 tgataggcgg tgagaacggt atgacgggcg agcgcacctt cggtttgcgc tgctcgatca
     8581 ttttttcata tcggggggat tggtgacgac tgactgggga tctgaggaga aggggagatg
     8641 cctggtcctg gcgcttatgg ccgccagcgt gctggtcctc gccgcttgtg gcggttctga
     8701 tgatgatggc gctgggcagg agcgaacgat cgcagccata gatgccgttg ccactcctcc
     8761 ggctgccgcc gaactgctcg gtgtcacatg gacaaccgcc gtcgacccgg agacgcgggt
     8821 cccgaagaac gaagtgacat cctttccaaa cgacgcacct gcgatcatcg ccgcggtgga
     8881 agtcggggag gtgcaggcgg gcacggagct gacagcgacg tggtcgatcg acggcgtaga
     8941 ggttcccaag gcgtcaatgc gagtcacggc acagcaggat ctgcaggagg ggtgggcaac
     9001 cttccagttc acccgagatc cggcccgtct cttcccgcta ggtgagcttg aggtacgggt
     9061 aacggctcct ggtgacgatg cggtcacggc aacggtggac attgtgctcc cggagaacta
     9121 atccggaagc gaccacggtg tttgggggga cgggaacgga cgcttcaacc agaccgatgg
     9181 tacgagaggc ttggcatgcg gctcgactgc tcccaaagag tagtcttctc aatgatcagc
     9241 gcggcccggg ccagtacccc gcatgcatac gaagaccccg ggcagtaccg tccggggtct
     9301 ttgcatgatt gttactcgtg aggaatgttc gggatgagct ccccggcgcg gatcggtacc
     9361 ttcaggcgat tctcaaacgg cgggatcggg cacgaccaac cactgttgta tgcacagtgg
     9421 gggttgtagg ccatgttgaa atcgaccacc agtcgcccat cgggccggac cttgggatca
     9481 aggtagcgcc cgacagaata ggtctcgtca tcggccgtgg tgtcgcggaa tggcaggaac
     9541 agtttgccgg tcttcgcatc ctggaaaact gaaagcgtcg cctcctcacc atccacctcg
     9601 aacctgatgc gcccgatgcg gatgtactgt tttggctcac cagtcatcgt gccgaccgta
     9661 atctcctcgc cgatgccgtc accgctttta tccagttcca ggatcagcga gagatcgggg
     9721 ttggtcggga agtaggaaag gccggtgaat gctactttct gctcctgcgt aaggggagac
     9781 tgttcctgct ccctgaaaaa agtgtccttg cgctcgcgga agccgctgag gcgccgctcg
     9841 tccgtactca tggatgaatg tctccgtgac caatgcagag ggtgtactcc acgctccgca
     9901 atctgatact ttctaaaagg aagtatagcc tgcacgtcaa taccggtcag ggcgtcaccg
     9961 aggatcagat cttctcgttc ttctcgattc ggtcggccgc cctggagttg cgatagttct
    10021 cgcggcttgc ctcgatctgc tgacgggcga aatccagacc gtaccagcca aggatgttcg
    10081 tttccttgtt ctcaagcagc ttgtaggtag cgaagaaggt ttcgatctcg cgcagccagt
    10141 gcttgccaac ctggtcgagc gacttgtagt gggcgaagcg gggatcgttg accggaactg
    10201 cgaggagctt aaaatcctcg cccttttcat ccctcatatc cagccctccg agggggcgcg
    10261 ccacgatggt acaacccggg aacgacggct cggtcatgag cacgcagacg tcgaggtggt
    10321 cgccatcgtc cgcgagagtg tccggaatga agccgtaatc ggtcgggtag tggacagagg
    10381 agtagaggac acgatcaagg aagaatgtgc cgctctcctc gtcgaattcg tacttgttac
    10441 ggctgccccg tggaatctcg ataaaggcta ggacagtttc ctcgcgggtt tcctcggcaa
    10501 cttcctgcgc ctcggcgtgg acattggcga cggtatcgct cgctgtcgca gtggtcttct
    10561 cgttcatgaa ccgtgactac tccacagtaa cgtctggcgt ggatgtgatc tcgatgcggc
    10621 tcctcgccgg ggcatagcaa ggtgatcagc atcatagaca gacgcattag gtcgaagctc
    10681 tggcggcgat tcggccgtgg gagccaggac acatcaattg ggtcgaatat cggtatagac
    10741 cgtatggtca ggccatggcg aagacctgcg atgtccagtc tggacggcga tggccagctg
    10801 gctgcgcgcc gtaatcaatg ggcccgcata ccagtcgata tagtaaccgt ttcaccgaaa
    10861 tggggcgccg gcagggtgtc cctcaccccg cgcgaggtcc ggagtgcacc tccccgggag
    10921 gcggcgacat cgagatctcg gtcacccatc gtcgggcggc aacctcggga gcgaccggcg
    10981 tctcctccag ggaataggcg ttggcgatct ccttcgtcaa ccacgcgacc aaatccatca
    11041 tgttcgtgag ggcgcaccgg atgctctcgg tgtcatgctc cgcccaggtc gtcgccaatc
    11101 tcatcgttac ctcctgaggt gcccactctt ccatgaatcg ggcgccgtgc cagagcgttc
    11161 cttttcgctc cggatgccag cggatgaggt gcattatcaa gcgacgctga tgcccgtcga
    11221 gacattcgtg agcgacgatc aaatcaccac ggcggagctt ctttgcgatc cagacgcagt
    11281 gataccagaa atcattgatc gtctcgtcga actgttcgta ggtgggtggg agaggttcac
    11341 actcggtgat ggttgtgatt cgtcgaagga tgggcgttag ccgttcgttt ctgtcgatca
    11401 gcacgcggta accacgggcg atggttgctc cgacctccgc gtggaggaga tcgcccggat
    11461 gcatcgcgtt cagttcgttg agcaggtctg caggtacgac ggagaagtcg acatcacggg
    11521 ctccgtcgaa gagcacgcgt cgttcgcgcc aggcgccaac tgccgtggcc tcaagaaagg
    11581 taatcaccgg atcgccgatg ttatgcagcc aggcatcatt ttctagcagg atttcaggat
    11641 cggtagcgaa gatcacaata tccagatcgg accacgcgtc agcagggtgg tcggtgcgcg
    11701 cccgcgagcc aaccaccatc gcggcctcga tagcaggttg cgccgtggcc catcggacga
    11761 agcgatccac gatgcgtccg tacgattcaa catggtctcc ggccatctcg cacgtcacgg
    11821 accgaccggt aaccccgcct gagccgcgat actgcgcacc gcgtgtgcac aggcacgcgt
    11881 tgcgccttca cgttgaccct tgagatcgaa gtgcgtttcg agcgagagta cgtcttcgta
    11941 gccgtcacgt ttgagcagcc ggaactgctc ctcatacccg atgattccgt cgccgatgac
    12001 cgtgaattcc actggttcac cagggcgaac cgcgtccttg agatggatgt gatggatccg
    12061 ctcccggacc gcctcatacc cttccgggat cggagcatga cctagggcgg cctcattgcc
    12121 gggatcccag atcaaaccga aggtcggatc cgggatgcgt tccagatacc aggcggcttc
    12181 cttcccggtg gcgatattgc atgagtattc gttctccagc ccgagcaggc ggccggcgct
    12241 ccgtgtgcgc acggtcgcct cctgcaggat cccgaggatc tcctcacgga cagacaccgg
    12301 gtcgtcaact ctccagaagc tgaaggcgcg gaccatgggc gcattaagga gagcggcgac
    12361 atcaagggac cgctccagga tgtcccactg ctcccttcgg gttgtggctt gcgcagagtg
    12421 ggttgcgcct gctgcagcgc cgtcaccaga gatgtggcac ttgagaaagg gcgaggcaat
    12481 gccgcagacc gaaaactctc gtgcacggat ggtatcggcg atgaccctga tcgatgcgcc
    12541 atcatgctcg acgatgctcg tattccagat gctgcgaagt tcaatgtccc tgatgccgag
    12601 gtcggtgcag acaccgaggg cggtatcaag atcctcgctg atctcatccg tgatgacgcc
    12661 gagccgggtc atggatcgtc ctttcgatta tccctgctga agatgtcgcc agcatcttag
    12721 ggagcgtgct gaccaacgtc aatcgccagg tcaaatagcc catggttggt ttgccggatt
    12781 tgcgcggcga gccggtacag gtcggcaagc tcaggacggt cggcatcctg taggaatgcg
    12841 atggcttcgt tgacatccag gacgtcgatt cgggtgatct gctcggcgcc gtggggattc
    12901 tccggcactc ctatgcgatg cacctcgccg aagcagacga ggcgctcgaa ttcggggtgt
    12961 acgaggtagt cgcgccacgg cttttcgtcg aaagagatgc acttgagggt ggcgaaggga
    13021 tagcaggact ccagtgcgta gccggtttcc tcccgcatct cccgcgagat agtctgcagc
    13081 agggtttcgc cgcgctcgcg tgtgccgcca gggagcatga cgtggccgtc tgccagggcg
    13141 ataacaatca tgcggtcacc gacaaaggga accatattga cgctctggag tcgggccgga
    13201 tccggttttc ctgttatcag gtggcagcca gtgagggcgg tgttgcccca gcgtacgccc
    13261 ctggcgagag ccggaaatcg tgcggcgagg tcaggatcgg ccactggtga gtgctcgtgg
    13321 gattggatca tgttgacgcc cttcgccagc cactcacgac gaacgcagga catgagcgga
    13381 gcaatgggac gactggatac gctgccagtc cctcaggatg accgtcccta gagcgtgaat
    13441 cgcccggtac gttgcaccgc cttccgccta ttcctgcccc gtgaggagat cctcccggat
    13501 cgccatgagg gaggctcgta gctggacatc gctgtctagc gccgactcga tcttcttgat
    13561 tccgtgaagc accgtggtgt gatcgcggcc gcccatcgag cgtccgatct cttccagtga
    13621 gatatcggtc tcctcgcgaa gcaggtacat ggcgacctgg cgcgggaaga cgatatctcg
    13681 cgttcgctgg cggcccttga ggtctttttc ctgaaccttg aaatgacgca ccacggcatc
    13741 gaccacgtcc gaggcggtgg tcttcgctcg acgctgggtg ttcgatgcgt cggtcagggc
    13801 ctccatggcg agggttaggt tcagacgccg gttgtagagc tgggccatca tcaggatctt
    13861 attcagcgcg ccctcgagct cgcggatgtt cgtctggtcg cgatgggcga tgtactcgat
    13921 gacatccgga ggcatgttaa caccaagctc ttcggcctta gtgcgaagaa tggccgtgcg
    13981 catctcaaag tcaggcgact gtacatccgc gatcagccca ccggcgaacc gggagcgcat
    14041 tcgctcttcc agcgctgcga tggagcgagg gggttggtcg gaactgacta tcacctgttt
    14101 gccgttctgg tagagcgcgt tgaaggtgtg aaagaactcc tcctgcgtgc tctccttgcc
    14161 ggcgatgaac tgaatgtcgt ccaccatcag tatgtcgatc gagcgataac ggccgcggaa
    14221 gtcctccatg cgctggctac ggatagcgtt gatcacgtcg ttcgtgaagg tttctgacgt
    14281 gacgtacatg atcttgagat cggggctgag ctccagggca cgatggccca cggcatggag
    14341 aaggtgcgtc ttgccaagac caacgccgcc gtgaacaaag aacgggttgt attgggctcc
    14401 tggccgctcc gcgatcgcct gggcggccgc gtgcgccaac cggttcgatg agccgacgac
    14461 atatgtctcg aaggtcaagc gtggaaccag gccgtgggcg gaggattcca gcaccagttg
    14521 tcgattaccg agccctgtaa ggctcctgtc ggcaacccgc tcgggccggc gtaccggttt
    14581 cggaggaggg gaaccaggag cgctgtcggc cgctcgtgcc gttgtcgccg cccgggcgcg
    14641 gcccggacga tcagccatct catgattcac ggtgaattcg accgaaatgc ggcggccaat
    14701 gacatcggaa agcgcccgct cgacctggga tgcgtagcgg ccctgtagcg ttgaggcgct
    14761 gaaggcgttg ggggcgtgga cgaccgcgat gtcgttgtcc accccggcaa ggcttgtctg
    14821 ccgaaaccag ttatcgaagg cgctccggga tatccgggac tcaagatcga agagcacggt
    14881 ctgccagagc tgattgacat tgatgctggc gtcgctggcg ccagtagtgt cagatgggtt
    14941 gtgctctctc ggagccgaca cgccgggatc tcccgcatac cagatgctca tgctgtcgtt
    15001 cctctcccca ccgattgcaa acgcaagggc ccggagagaa gagtgtacgt acactcggtg
    15061 cgtctgtcca gcccctcggc gattttgtct cggttgacag tgccgggagc atccattcga
    15121 gtatcccata atacgatgat gtgggggtga cactctcctt gctgcccagc cagagcttgg
    15181 gttctccgga cgatattgat acactctggg gttgaacccc gcggacagcc gtgggtagcc
    15241 tgttcatccc ttcggcgact tgccatcccg cattcccgca tactcccagg tcagacccgc
    15301 tatgagcacc ttcatgtccg agacttttga gacgaccctg ccagccgccg atctggccga
    15361 tgttgccgac ctcctcagcg aagggaagat cggtcctcgc gacggcgcgg gcaaaaccgt
    15421 ggttgtggcg atgagcggtg gcgtcgacag cgcagtgacg gcgctcatca tgcgcgagcg
    15481 cggctacaac gtcgtcggca tgaacttgcg gctcttctct ccagaggatg aggatcaccg
    15541 ggcgaatcca tgctgcggca tacctgcgat ggatgacgcc cgagccacct gcgcgacgat
    15601 cggcgtaccg ttctacgcca tcaatatgga gatcgagttc ggcggggcag tcatccagaa
    15661 attcatcgat gaatatgccg cgggccggac accgaaccca tgccttgagt gcaaccgcca
    15721 tgtgaagttc cgccatctgg ttcagcgcgc gaagttgttg ggtgcgggct gtctcgcgac
    15781 cgggcactac gcccgcatcg aacgcggaga gccggcatct gggattccac accgccttta
    15841 tcgcgcactg gatgatcgga aggaccaaag ttacgttctc tacacgctca accaggagca
    15901 gcttgactat atccagttcc cgcttggtgg cctgaccaag ccggagacgc gtcgcttggc
    15961 gcgtgcctat ggtctccctg tggccgacaa ggccgagagc caggagatct gcttcgtcgg
    16021 gaaccgctcc tatgccgatt ttgtcgcgga gcggcgcccg gatgtgacga agccgggtga
    16081 gatcatcacg aaagccggcg aggtgatcgg ccagcacaat gggttggtga accacacggt
    16141 cggccagcgc cggggtatac gcgttgccgc gccgaagccc tacttcgtgc tgcaccttga
    16201 tacggatgca aaccagcttg tcgttggcga tcgcgaggat gccgaggcaa aggcgctacg
    16261 agtcgatggc gtggcaatgg catccggtgg ctggccgcat gagccgttcg acgcggaaat
    16321 cgtcgtgcgc tatcggggcg tgccaagcgc tgctcgagtt cagcttgaat cgtccggaca
    16381 gcgtacggcc catatcaccc tgctggggga tcgcggtcca atcgcttctc ctggccaggc
    16441 cgtcgtcttc tatcgcggcg atgaagtctt gggtggcgga acgatctgcg atgtcgagcg
    16501 acgcggcaac gaggagctca gcgcctgatc ccatggtttc tgctgatacg gtctaaaggg
    16561 cggggcccga gcaccctttt cgcaggcgcc accgtacctt gtgtccccgg tggagcaccg
    16621 cgctggttga aagagaggta tctccttgag tggtcgtagc gagtctccag gccaatcaac
    16681 tgtgtcccac gatgttgacg tctggtggaa acacgccgtg gtttaccaga tctatccacg
    16741 gagcttcacg gattcgaacg gcgacggcat aggcgatgtc ccgggcatca tcgaaaagct
    16801 ggattacctg gccgagctcg gtgtcgatgt cgtatggctc tccccgatct atcgctcccc
    16861 gcaagacgac aatggctacg acatcagcga ttaccaggat atcgatccga tgttcgggac
    16921 gctggaggat gtcgacaggc tgctgcagga ggctcaccgg cgaaacatca agatcttgat
    16981 ggacttggtt gtcaaccaca catcagacga gcatccttgg ttcatcgagt cccgatccag
    17041 catcgacaat cccaagcggg actggtactg gtggcggcct gcacacgagg gcatgaccgc
    17101 tggtgcaccg ggcgccgaac cgagcaactg gcgatcgctc ttcagcgggt cggcatggga
    17161 gttggacgag acgaccggcg aatattacct gcatctcttc tcccgccgtc agcccgacct
    17221 gaactgggag aacccagatg tccgggaggc catctacgag atgatgaact ggtggctgga
    17281 tcgcgggatc gacggttttc gcatggacgt cattaacatg atctccaagg agacatcgct
    17341 gcctgacggt catgtgaccg ggccggggtt ctatggtgat ggatctccct tctttatgaa
    17401 tggtccccgt agtcacgaat ttctgcagga gatgcatcgc gaggtgtttg ccggtcgcga
    17461 ggcccggctc ctaacagtgg gcgagatgcc gggtgcgacg gtcgatgaag cgctcctcta
    17521 taccgacccg gaacggaacg aacttgatat gatcttccag ttcgagcaca tggaattgga
    17581 tcatgtcacc agtcgctacg ataccaagcc actgaatctg acagatctaa aggcgtcact
    17641 tggtcggtgg caagttggcc tcgctgatgt aggctggaac agcctctact ggaacaacca
    17701 cgaccagccc cgcatagtct ctcgcttcgg tgatgacggc gaataccggg ttgagtccgc
    17761 gaagatgctc gggacggtcc tgcacatgca ccgcgggacg ccctacgtct atcagggcga
    17821 ggagatcggg atgaccaaca tgtcattcac cagccgcgcc gacttccagg acatcgcaac
    17881 gatcaacttc taccaagaag cgcttaagca cggcactgtc accgatgatc tgtttgagaa
    17941 gctggcagcc ggaacgcgcg acaatgcccg gacgccgatg cagtgggata gctcagagaa
    18001 gggtggtttc acgactggtg tgccgtggtt cccagtcaac ccaaatacct cagagatcaa
    18061 tgtcgacgcg gcgatggctg atgagcactc cgtcttccac cattaccgca agctcattgc
    18121 gctacgccac gatcttcccg tggtcgcgct gggcaatttc aacatgctgc tggagtggga
    18181 cccccatatc tatgcgttta cccgctcgct gaatggcgta gagttgctgg tccttggcaa
    18241 cttctccgct aaggagctgc cggttgatct gcccgatggg gacgcatggg cgggcgccga
    18301 gctgttgctc gggaactaca atgaggtgag cgccgacgca cccagcatcg ttctgcaacc
    18361 gtgggaggca aggatctacc ggcgggagca aggtgagacg ggcaacgctt cctgattcac
    18421 tcatggaaca gttgttgcgt ccgtagggca tccagaagag tggcatgctg ccagaggtct
    18481 gcctcggccg gtattgtatc ggccgtgcaa ggggcaggga gcaggaaagg gagtttggga
    18541 gttgaagcaa aaggccatca tcggcagcgt tgttgccgcg gtcgtattca tcgccatcat
    18601 cgtggggttg tacctgctgg gtggggaaga tcagtccgcg cttgagcgcc tccgggacat
    18661 cgcaatcatc tttattgtgt tgctctcgct ggttacggtg atcctgcttg cgggcatcac
    18721 ggccgcgttg gcgttcctgg ccttccagat caaggatcgg gtgatcccgc tgcttgagga
    18781 agtcactgga accgtgaacc gggttcgcaa tactacgaat tacatgacag aggaagcggt
    18841 caagccgcta atttcggttg ccagcaactt ctcgaagatt cggcagatga gcaagacggt
    18901 gacaggcaag cgcaagcggc caccaaagcc gccgacttat cgctccgctg aggaggcatc
    18961 aaggtaagga ggtcgagtaa tggaagagtt tttgaagaat gcccggaagc agcttgaaga
    19021 gctggatctt cagggccagg cgaaagatat cgacctgggt aagctcaagc agctgacgct
    19081 gcgcgagcag cagcagcggg aagaggaggc cgcttccgaa ggtttcttgg gcggcatgtt
    19141 gctcggcatc gttgtcggag cgctcctcgc gttgatcttc gccccaaagt cgggaagtga
    19201 gacccgtgag atggtagccg ggaccgccag tgagctgaag cacaaggccg aggggctggt
    19261 gggccaatcg aaaaacggga gcaccgatca ggcggcttcg acgctcagcg acgaaccagc
    19321 gatcgagcgc gagatcggcg atacttctac gcgatcgacc cccggcgtca tatagtctag
    19381 gcatactctg taggacaaga cgaacaacgc cccggatatg ccgtatccgg ggcgttcttg
    19441 tatgtgtgca tactggtctc gattagcagc attctttggc aaaaataccg aatcaggtgg
    19501 agcagcgcgc ctgcaggcgg tcgcaagagg tcgcctcatg gggggaccaa gtggtgccca
    19561 agatgatcgt gatcatgctc acgtgcaaag aggggcgcgg atcgggttgg gcgaatgcag
    19621 ggacacgccg aatggcgcca tggccggaac ccttggtcat cagctgtgag aaactcggga
    19681 gggccggtca tacggaatcc ggataacgag tgcgtttccg gcggagggat cggtccccgg
    19741 atgcgtggcg cgatactctg cggcgcggcg tcgggtcgac atagacggac cgcatcggca
    19801 gttaggcaaa gccgcaggca tcgagtgtga gcggcccatt gatgcctccg gcatcgcctg
    19861 tgggtcgacc cctagacgta cacccgtgca tgagccggat tccgtattat ctatacgcag
    19921 tgagggaagc gacatgcgcc atggtgggag tcgatctccg gcaacggacg atgcagaggc
    19981 aattggggag cacttgacgg gccgctcccg cgccgcggca tcctgtgcca catgatccgg
    20041 gtatcgacgc gtgcggcgtc gattgggccg acattctctc ggaagtgcga cccttacgcc
    20101 gggtaataca tcctttagcc gaagtcggta acagacctct tagagcccca gtttatctca
    20161 gcagtgttgc tccccatccg ccacatctcc gccacaacgc gtttgccgga gcatcggtat
    20221 ggagcccgac gagagggcac gggcaaagta accggggagc acttcaccta tgcacagcgt
    20281 gaggattggc gtactcatca tctctgagga ggattcagca atcgcatggt aacgggatga
    20341 acgagatgaa cgcccccacc acgatgctga gggcgttcat ctcgttcatt gatccaggga
    20401 aggacttggg gcaaggtcac cgtgacggac gctctgtcac ctgcaggttt gttcaaacat
    20461 cgccgagaga ttcactcctc cgccggtcgc acggacgctc accaccgtct cgccataagg
    20521 aagggcagca ccaccgccga aagtccactc aaaaagccca tcttgcagca cgaaatactc
    20581 ggtaaccggg tagtctacga tttcaccatt gctgtacgtc aaaatgacgc gataaggctg
    20641 gtagacctcc cgcaccgtga cgtgaacacc gcactctctt tcctttgtca ccgggttgat
    20701 gtaccactgg gtcacgtcgt aggtaattga cgggacgacg acgccattgg gcatgctgtc
    20761 gcaggcgttc ccgatgccgt ccccgtcggt atccgcctga tcggagttag cgacgctgcg
    20821 gcagttgtcg gcgtagttct ggataccatc gaggtcggtg tcgccattgt ccacgctatc
    20881 acaggcgtcg ccggtaccat cgctgtcggt atcttcctgg ccggggttgg cagtagtcgg
    20941 gcagttgtcg gacgtatcgg ccacgccatc gccgtcggta tcaccggtcg gtgtgctgtc
    21001 gcaggcatca gggatgtcgt cggtatcggt gtcaacacca tcatcgccag ccgcacagac
    21061 atcgaggttg ttgtcgaccc cgtcaccatc gtcatcgccg ttgggcgtca gatccacggc
    21121 gttgtccacg ccgccgccat cggtgtcccc ggtcggcgtg ctgtcacagg cgtcggcggt
    21181 gccatcgctg tcggtatcct cctggccggg gttgtaggcg gcacagttat cgttgctgtc
    21241 cggcacaccg tccccgtcgc cgtccccgtc aagcaccccg ccagctgcga cgtagctggc
    21301 acactcgccg ggattggtga accgcgagcc atcttcgatg cgcaccatgc tggcgtgccc
    21361 accacctgaa cacgcacggg ctgcctcgct atcgccctgc gctgatccgg ccaggggcag
    21421 tgatgccgcg accagcagca gcagcaggac catcgacagg atctgccgca cccgtcgggt
    21481 ccgcgttcgc tcgcgaggac cgcccaccgt gatggtgccg cgggagaccc gatggccctc
    21541 gacctcatcc tggggctgct ccacggcctt gctgctcggg gtcgttgctt catacatcat
    21601 cgtgctgctc cttgcctgcg tgttgtggaa gatcgtctcc cgacctccct tccaaggtag
    21661 ggagcctgga ggggatgggc agccgcctgc gacacacctc tcctgtgtcc tgcgacacac
    21721 ccgcaacggg cacgacaacg gccctcgaag gtatcgagag ccgtgtcgat agctgctgtt
    21781 gcatgtagga gatcccgacg cgctctacgc
//
LOCUS       DASYWM010000247        16574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_123893, whole genome shotgun sequence.
ACCESSION   DASYWM010000247 DASYWM010000000
VERSION     DASYWM010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 16574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16574
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123893"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..166
                     /locus_tag="VGR29_10020"
     CDS             <1..166
                     /locus_tag="VGR29_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV2073965.1"
                     /translation="IIHLPEGTEMVMPGDNIQMGVELITPVAIEEGLRFAIREGGRTV
                     GAGAVTSITE"
     gene            243..566
                     /gene="rpsJ"
                     /locus_tag="VGR29_10025"
     CDS             243..566
                     /gene="rpsJ"
                     /locus_tag="VGR29_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV2073966.1"
                     /translation="MVAARKTAQKIRIRLKAYDHKILDQSAGRIVETAESTGAKVAGP
                     VPLPTRIEKFTVMRSPFIDKDSQEHFEIRTHKRLIDVLEPSGNTIRALMRLNLPAGVD
                     IEIKL"
     gene            691..1320
                     /gene="rplC"
                     /locus_tag="VGR29_10030"
     CDS             691..1320
                     /gene="rplC"
                     /locus_tag="VGR29_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV2073967.1"
                     /translation="MVHGLIGRKLGMTQIFDEQGLARPVTVIEAGPCVVTQIRSEEKD
                     GYEAVQLGYGIARKLNKPEQGHVKASGHMVKTLREVKADNYGDIEVGQVFKADTFAEG
                     EVVDITGTSKGRGFQGGMKRHGFSGAQKTHGQSDRTRAPGSIGSSATPGRVFKGMRMA
                     GHMGHARVTVQNLKVLRVDTERNLLLVEGSVPGPNKGTLLIRRAVKGRK"
     gene            1358..2092
                     /gene="rplD"
                     /locus_tag="VGR29_10035"
     CDS             1358..2092
                     /gene="rplD"
                     /locus_tag="VGR29_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV2073968.1"
                     /translation="MTAMGARTTMQIDVVNTRGEVVGQTALDETVWGIEPHMAVMHQA
                     LVRQQANARLGTADTKTRGEVSGGGRKPWRQKGTGRARQGSIRSPQWKGGGVVFGPHP
                     RSYTQAMPRQMRRLAIRSVLSAKLRDDRLTVVQGLGDIEGRTKVMKSVLEAMPASRSV
                     LIVLPENVDTIVRAAANLPQTKTIIAPMLNVRDVLKFDRLIVTEEAIEVIEKLWALPE
                     EKRQPSTWKLERQAASELPDSAEEVA"
     gene            2095..2406
                     /gene="rplW"
                     /locus_tag="VGR29_10040"
     CDS             2095..2406
                     /gene="rplW"
                     /locus_tag="VGR29_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV2073969.1"
                     /translation="MAELRLEDVIRRPVITEKNSWLMDKDQYTFEVHPDSTKIQIKSA
                     VQDAFGVRVKAVNTLNVKPKAKSRMIRRGAGRISGMGRGWKKAIVTLHPGERIDIFEQ
                     F"
     gene            2432..3265
                     /gene="rplB"
                     /locus_tag="VGR29_10045"
     CDS             2432..3265
                     /gene="rplB"
                     /locus_tag="VGR29_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV2073970.1"
                     /translation="MPVRKYKPTSPARRSMSVSTFEEITKKRPEKSLIEALKKHSGRN
                     NRGRITTRHRGGGNKRFYRIIDFKRNKLDVPARVAAIEYDPNRSARIALLFYADGEKR
                     YILAPLGVRVGDTIVAGENAPIRTGNALPLRNIPTGTQIHNIELYPGKGGQLCRSAGT
                     SAQLMAKVNNYAQLRMPSGEVRLVHLTCMATLGQVGNVDHENIEIGKAGRSRHMGRRP
                     TVRGSVMNPSDHPHGGGEGKAPIGGQPKTKWGKPAFQRTRNNKRTESMIVRRRPANKR
                     R"
     gene            3343..3624
                     /gene="rpsS"
                     /locus_tag="VGR29_10050"
     CDS             3343..3624
                     /gene="rpsS"
                     /locus_tag="VGR29_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV2073971.1"
                     /translation="MSRSSKKGPYIDPKLQKKVDVLNQSNQRTVIRTWARACTIFPGM
                     VGHTIAVHNGRQHVPIYVTEPMVGHKLGEFAPTRTYRGHGKDADRRGRR"
     gene            3656..3997
                     /gene="rplV"
                     /locus_tag="VGR29_10055"
     CDS             3656..3997
                     /gene="rplV"
                     /locus_tag="VGR29_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV2073972.1"
                     /translation="MEVRASAQRVRISPRKVRLVVDMVRGKKVSDAIQMMKFVPNRAA
                     VDVEKLLKSVSANAENNYDLDPDNLWIKAIYADDAPQLRRFKPKARGRVGKILRRSCH
                     ITVIAEERGGR"
     gene            3997..4680
                     /gene="rpsC"
                     /locus_tag="VGR29_10060"
     CDS             3997..4680
                     /gene="rpsC"
                     /locus_tag="VGR29_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV2073973.1"
                     /translation="MGRKVNPVGFRLGIASDWESKWFAEKQYTELLHEDLAIRKLVMN
                     ELTRAGIARIELERAANKVDVTLHTAKPGIVIGKQGANVERLRQLIEKNVAKKVNIRI
                     EEIKVPETNARLIAESISEQIQRRVAYRRAMKHAIQQAMRRGAKGVKVRLSGRLGGAE
                     MSRTVSEMEGRVPLHTLRANIEFATVHAHTTYGRIGVKVWVYKGDVMPQKAEITEASL
                     TADLAKAGD"
     gene            4749..5192
                     /gene="rplP"
                     /locus_tag="VGR29_10065"
     CDS             4749..5192
                     /gene="rplP"
                     /locus_tag="VGR29_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV2073974.1"
                     /translation="MLMPKRTKYRKVMRGRMTGKAYRGSTISFGDIALQSIEPAWVSS
                     RQIEASRRAITNYLKRGGKVWIRIFPDKSVTQKPAETRMGSGKGSPEYWVAVVKPGRI
                     LFEVGGVDNERASEALRRAAMKMPMKCKVIIRETPGAQTTEEGVA"
     gene            5189..5392
                     /gene="rpmC"
                     /locus_tag="VGR29_10070"
     CDS             5189..5392
                     /gene="rpmC"
                     /locus_tag="VGR29_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV2073975.1"
                     /translation="MKPSELRGMTEEQLEARLKELHTEWRDLRFQDAVGNLTATSRIR
                     EIRKDIARIHTIRTEQANQAVSQ"
     gene            5419..5727
                     /gene="rpsQ"
                     /locus_tag="VGR29_10075"
     CDS             5419..5727
                     /gene="rpsQ"
                     /locus_tag="VGR29_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV2073976.1"
                     /translation="MSEVQDAATPIEAAVNRLQKPRTQKVGRVVSDKMDKTVVVAVDY
                     VRRHPLYHKRITRTSKFFAHDESGNCKPGDLVRIEECRPMSKRKRWIVREVVERAVQI
                     "
     gene            5785..6153
                     /gene="rplN"
                     /locus_tag="VGR29_10080"
     CDS             5785..6153
                     /gene="rplN"
                     /locus_tag="VGR29_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV2073977.1"
                     /translation="MIQTQTRLKVADNSGAREIMCIRVLGGSSPKFGSVGDTIIASVK
                     AAQPNAAVKKGDVVRAVIVRTASEYGRPDGSHIKFDDNAAVLINQQGAPRGTRIFGPV
                     GRELRERQYMRIVSLAPEVL"
     gene            6160..6486
                     /gene="rplX"
                     /locus_tag="VGR29_10085"
     CDS             6160..6486
                     /gene="rplX"
                     /locus_tag="VGR29_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV2073978.1"
                     /translation="MMRKILAGDEVLVISGRNKGSRGVVRKNLTDVDRVVVEGVNIVK
                     KHIKRGRARQAGIVEVEAPFHVSNVKVVCPSCGEPTRIGVRANDEGKNERHCKNCDAV
                     VAQQSR"
     gene            6631..7170
                     /gene="rplE"
                     /locus_tag="VGR29_10090"
     CDS             6631..7170
                     /gene="rplE"
                     /locus_tag="VGR29_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV2073979.1"
                     /translation="MARLHDRYKEDIAPALTSEFGYKNPFQVPKLEKIVLNIGLGEAV
                     ANGRALDAAVNDLTTITGQKPVVTRAKKSIASFKLREGMPIGAMVTLRGARMYEFMDR
                     LVATALPRIRDFRGISPNAFDGRGNYTLGLREQIMFPEIDYDQIDKVRGLEISIVTSA
                     RTDEEGRRLLALLGMPFAR"
     gene            7191..7376
                     /locus_tag="VGR29_10095"
     CDS             7191..7376
                     /locus_tag="VGR29_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEV2073980.1"
                     /translation="MAKKSSIARAARPPKYSTRSVNRCRLCGRSRAYMRKFGVCRICF
                     RELASLGRLPGVTKSSW"
     gene            7432..7830
                     /gene="rpsH"
                     /locus_tag="VGR29_10100"
     CDS             7432..7830
                     /gene="rpsH"
                     /locus_tag="VGR29_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010169919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV2073981.1"
                     /translation="MSVNDPISDMLTRIRNAGMAQKRETSMPATKILIAIAGILKSEG
                     YIEDFQVIEKRPQDQLVLTLSYGPDKRHTIREIKRISKPGLRVYAGKSELPRVKSGLG
                     IAIVSTPDGVLTGYEARRRGIGGEVLCTVW"
     gene            7892..8437
                     /gene="rplF"
                     /locus_tag="VGR29_10105"
     CDS             7892..8437
                     /gene="rplF"
                     /locus_tag="VGR29_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV2073982.1"
                     /translation="MSRIGIQPIVVPNGVNVEIGSDNAVQVRGPKGQLSQKLTPRLQI
                     VQEDGSVRVVRTSEEREVKALHGLTRSLLNNMVVGVTEGYTKTLEIQGVGYRAQMQGK
                     TLVLNVGFSHTVPIVPPEGITFQVEGNNRVLVSGIDKQAVGEEAARIRGVRPPEPYKG
                     KGIRYAGEFVRRKAGKAGKAK"
     gene            8525..8899
                     /gene="rplR"
                     /locus_tag="VGR29_10110"
     CDS             8525..8899
                     /gene="rplR"
                     /locus_tag="VGR29_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV2073983.1"
                     /translation="MRLKYRRALSQRERRHKRVRAKVNGTAARPRLNVYRSSAHIYCQ
                     IIDDVKGHTLVAASDLEDDVRERAGDDAAKVARARAVGEVIAQRAREAGVDAVVFDRG
                     GFLYHGRIKAVADGAREAGLKF"
     gene            8961..9470
                     /gene="rpsE"
                     /locus_tag="VGR29_10115"
                     /pseudo
     CDS             8961..9470
                     /gene="rpsE"
                     /locus_tag="VGR29_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            9693..9905
                     /gene="rpmD"
                     /locus_tag="VGR29_10120"
     CDS             9693..9905
                     /gene="rpmD"
                     /locus_tag="VGR29_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV2073984.1"
                     /translation="MSDTQSTGNGQLRITLVRSTIGRPGDQERAVKSLGLRKVNHTVV
                     RNDDPSIRGTVNKISHLVRVEEVAAE"
     gene            9956..10396
                     /gene="rplO"
                     /locus_tag="VGR29_10125"
                     /pseudo
     CDS             9956..10396
                     /gene="rplO"
                     /locus_tag="VGR29_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            10552..11859
                     /gene="secY"
                     /locus_tag="VGR29_10130"
     CDS             10552..11859
                     /gene="secY"
                     /locus_tag="VGR29_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV2073985.1"
                     /translation="MLQAVINAFRIPDLRRKILFTIAMLVVFRLVASVPIPGVDRQGL
                     RDFIETNQLLGMLNLFSGGGLNNFSIAALGVYPYITATIIMQLMTPIIPRLNELSMEG
                     QQGRNQIDRYTHYLTVPLALLQGYGQMLLFASDQSGNLVQNFGLFDADTFLPTLAILL
                     TLTAGTMLLVWIGELITENGIGNGVSIIIFGGIIASLPSAIGGLVSGGTLGENVAGTV
                     FFVAISLLTIVGVVLINEGQRRIPVHHAKRVRAGRQYGGNTTNIPLKVNSAGMIPLIF
                     AVSIMVFPGMVANFLQSSGTDWVRSLAVNISQWLSPNTVPYNIVYFFMVVGFTFFYTM
                     VVFQQQRIPDSLQRQGAFIPGIRPGRNTAIYLQRVLTRITIVGALFLGVIAILPFIAA
                     RITGVQNLLLSSTSFLIVVGVAIDTMRQLEAQLMMRNYEGFID"
     gene            11930..12616
                     /locus_tag="VGR29_10135"
     CDS             11930..12616
                     /locus_tag="VGR29_10135"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV2073986.1"
                     /translation="MYVILMGAQGSGKGTQAALIGPKLKLVKVATGDLFRAAIAQKSE
                     LGLQVQAILESGNLVPDELTNAIVQVRLAQIAAAKAKGEDVAGALFDGFPRTEPQARA
                     LDEILAHQGESLTAVVEIDVPREKLVARLAGRRVCESCGTVYHVEADPPVVPGICNKC
                     GGKLLQREDDTPEAITRRLELYDDLTRPLLAYYAEQGVLERVDGDQDIQKVEQDLLDA
                     IQSRAESMKA"
     gene            12624..13391
                     /gene="map"
                     /locus_tag="VGR29_10140"
     CDS             12624..13391
                     /gene="map"
                     /locus_tag="VGR29_10140"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEV2073987.1"
                     /translation="MPITIKNEREIEKMRAAGQVVALCHQRVEAAIAPGVTTADLDAL
                     VRDTVKEHGAIASFLGYNGFRGNICASVNEEIVHGIPGSRVLGDGDVIAVDIGAILDG
                     WHGDSAWTYPVGEVSDDARRLLADTEEALKVGVAAARAGNRLGEIGHAIEAYAAPRGY
                     GIVRGYGGHGIGRRMHEDPHVPNYGDRTYGPVLRNGMTLAIEPMLTLGTDETRELEDG
                     WTVITADGTLSAHFEHTVAITDEEPIVLTKRLVSVVK"
     gene            13635..13748
                     /gene="rpmJ"
                     /locus_tag="VGR29_10145"
     CDS             13635..13748
                     /gene="rpmJ"
                     /locus_tag="VGR29_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV2073988.1"
                     /translation="MKVSASVSKRCDRCRIIRRKGIVRVICTNPRHKQRQG"
     gene            13817..14185
                     /gene="rpsM"
                     /locus_tag="VGR29_10150"
     CDS             13817..14185
                     /gene="rpsM"
                     /locus_tag="VGR29_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV2073989.1"
                     /translation="MARIAGVDLPREKRVEVGLTYIYGVGLHTSQRLLEQTGINPDTR
                     VRDLTDDEANRLRDVIDREVRVEGDLRREISMNIKRLMDIGSYRGVRHRRGLPLRGQR
                     TRTNARTRRGPKRAIGGRKK"
     gene            14292..14696
                     /gene="rpsK"
                     /locus_tag="VGR29_10155"
     CDS             14292..14696
                     /gene="rpsK"
                     /locus_tag="VGR29_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV2073990.1"
                     /translation="MSERRRGTAKSRVRRRDRKNIPRGKAYIQATFNNTIVTLTDPNG
                     NVIAWSSAGANGFKGSRKSTPFAAQMTAEAAARRAQEHGLRQVDVYVRGPGSGREMAV
                     RSLQASGVQVISITDTTPIPHNGCRPPKRRRV"
     gene            14821..15873
                     /locus_tag="VGR29_10160"
     CDS             14821..15873
                     /locus_tag="VGR29_10160"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV2073991.1"
                     /translation="MLQQPEFTIKTVEAGRNYGRYQVEPLEPGYGTTLGNSLRRILLR
                     SLQGCAVSRIRIDNVWHEFATVPNVREDVTEIVLNLKSVRLRRVTELRGDVRAHLYAR
                     GEGDGERVVTAGDVEWPVEVDLVNPDQPIATLDGPDAVLDMDLWISRGRGYRPAEAQE
                     TVALGEIPIDSIFTPIERVNYVIEQTRVGSEVDYDRLILEILTDGTIDPDDALSQSAK
                     ILMDHARVFADFTRPEPEIAANSFISEEVQNKPLADLGLSPRVLNALRSKQIDKVGQI
                     LTMDPDQLLSIRNFGPRSLTELREKLTEFGYLSDVPAIDLAAYGEDDSEADFVDALAQ
                     VAEASAVDEDSTNEAP"
     gene            15860..16213
                     /gene="rplQ"
                     /locus_tag="VGR29_10165"
     CDS             15860..16213
                     /gene="rplQ"
                     /locus_tag="VGR29_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV2073992.1"
                     /translation="MRHRKQGRKFGRNPAQRKALLRQLAISLILNERITTTEAKAKEL
                     RSVVEKLVTIAREDSSHSRNIVMSRLDHEPSVNRLFDEVAPRFEGTPGGYTRMAKLGV
                     RKGDAAPMVLIEFVS"
     gene            16279..>16574
                     /locus_tag="VGR29_10170"
     CDS             16279..>16574
                     /locus_tag="VGR29_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073993.1"
                     /translation="MAATLKFTLAYDGTGFRGSQRQKNGRSVQEDLETALSRLNGRAT
                     QVVLAGRTDSGVHAVGQVASIEDVRPDLPDHRMVQAINAHLAEDLAVLGCERREG"
BASE COUNT         3522 a         4662 c         5392 g         2998 t
ORIGIN      
        1 gatcatccac ctgccggaag gcacggagat ggtgatgccc ggcgacaaca tccagatggg
       61 ggtggagctg atcacgccgg tggcgatcga ggaggggctg cgctttgcga ttcgcgaggg
      121 cggccggacc gtcggcgccg gcgccgtcac ttccatcacg gagtaagttt ttattgacca
      181 cggtggccgg tgtgccgcga tgagcggtat accggccact ctgagtgtga gggattctgc
      241 caatggtagc ggcgcgtaag acggctcaaa agatccgtat ccgcctgaag gcctacgatc
      301 acaagattct cgaccagtcg gcggggcgaa tcgtcgagac cgctgagtcg accggcgcca
      361 aggtcgcggg accagtgccc ctgccaacgc ggatcgagaa gttcactgtc atgcgttctc
      421 cgtttatcga taaggactcc caggagcact ttgagatccg gacgcataag cgcctgatcg
      481 acgtgctgga gcccagcgga aacaccattc gcgccttgat gcggctgaat cttccggccg
      541 gcgtggatat cgagatcaag ctctaggtat tgatggaaac cggaatgtgg ccaggaggcc
      601 aatagccggg atagcggcac aacacgctct gcgaacggtt gcggcacgag ctgcttcatc
      661 cgtttcctga ctcagagcag gggagagcca atggttcatg gattgatcgg gcgcaagctc
      721 ggtatgacac agatatttga tgagcagggg ttggcgcggc cggtaacggt cattgaagct
      781 ggcccgtgcg tcgtaaccca gattcgcagc gaagagaagg acggctatga ggccgtccag
      841 ctgggatatg ggatcgccag gaagctgaac aagccggagc agggccacgt aaaggccagc
      901 ggccacatgg tcaagacgct tcgggaagtg aaggccgaca actacggcga tattgaagtt
      961 ggacaagtct ttaaggctga tacgttcgcc gagggcgagg tcgtcgatat caccggtacc
     1021 tcgaagggcc gagggttcca gggcggcatg aagcgtcacg gcttctccgg cgcccagaaa
     1081 acccacggtc agtcggaccg gacacgcgct cctggttcga ttggatccag cgcgactccg
     1141 ggtcgggtct ttaagggtat gcgcatggct ggtcatatgg gccatgcccg cgtgacggtg
     1201 cagaatctca aggtccttcg cgtggacacc gagcgcaatc tgctgctcgt ggaaggatcg
     1261 gttccgggcc cgaacaaggg gacgttgctg attcgacgtg ccgtgaaggg ccggaagtag
     1321 ccgccagttc ggcgcctgaa ggggcgcgtt ggtgagcatg acggctatgg gagcaagaac
     1381 gacgatgcag attgatgttg taaatacaag aggcgaggtc gtgggacaaa cggcgctcga
     1441 cgaaacagtc tggggtatag agccccatat ggcggtgatg caccaggcgc tggttcgcca
     1501 gcaggcgaat gcccgccttg gtaccgccga cactaagacc cgcggtgaag taagcggtgg
     1561 tggacgcaag ccgtggcgcc agaaggggac aggacgcgcc cgtcagggat cgatccggtc
     1621 accacagtgg aaaggtggcg gcgtggtctt cggaccgcat ccgcgctcat acacacaggc
     1681 aatgccgcga cagatgcgcc ggctcgcaat tcgatcggtg ctgtcggcga agctgcgtga
     1741 cgaccgcctc accgttgtgc agggtcttgg tgacatcgag ggtcgcacca aggtgatgaa
     1801 gtctgttctg gaggcgatgc ctgcatcgcg ctcggtactg atcgtgctgc ctgagaacgt
     1861 tgatacgatc gtacgcgctg ctgcaaacct gccgcagacg aagacgatca tcgctccgat
     1921 gctgaatgtt cgcgatgtcc tcaagttcga ccgtctcatc gtgaccgaag aggcgattga
     1981 agtcattgag aagctctggg cgctccctga agagaagcgt cagccgagca cctggaagct
     2041 tgagcgccag gccgcatccg agcttccgga tagtgccgag gaggttgcct agacatggca
     2101 gagcttcgtc tagaggacgt catccgtcgc ccggttatca ccgagaagaa ttcctggctg
     2161 atggacaagg accagtacac gtttgaagtg caccccgact cgaccaagat ccagatcaag
     2221 tccgcggtgc aggatgcgtt cggggtgagg gtgaaagccg tcaacacgct gaacgtcaag
     2281 ccgaaggcaa agtcgcgaat gattcgccgc ggcgccggca ggatttccgg catgggccgg
     2341 ggatggaaga aggccatcgt gacgctccac ccgggcgagc ggatcgacat cttcgagcag
     2401 ttttaacatc gggtagaagg gaacgtttgg aatgccagtt cgcaagtaca agccgacctc
     2461 cccggcgcgc cgctcgatga gtgtgagcac gttcgaggag atcacgaaaa agcgacctga
     2521 gaagagcctg atcgaggctc tgaagaagca ctcgggtcgc aacaatcggg gccgcatcac
     2581 aacccgccat cgcggcggtg gaaacaagcg gttctatcgc attatcgact tcaagcggaa
     2641 caagctggac gtcccggccc gagtggccgc gatcgagtac gatccgaacc gttcggcccg
     2701 gatcgccctg ctgttctacg cggacggtga gaagcgctac atcctggcgc ccctcggcgt
     2761 ccgggtcggc gacacgatcg ttgccggcga gaacgcgccg attcgcacgg ggaacgcgtt
     2821 gccgcttcgg aacatcccga cgggtacgca gattcacaac atcgaactct acccgggtaa
     2881 gggtggccag ctttgccggt cggcgggaac atcggcgcag ctgatggcga aggtcaacaa
     2941 ctacgctcag ctccgcatgc cctccggtga ggttcgcctc gtccacctga cctgtatggc
     3001 gacgctcggg caggtgggca atgtcgatca cgagaatatc gagatcggta aggccggtcg
     3061 tagccgccat atgggccgtc gtccaactgt ccgtggatcc gtgatgaacc ccagcgatca
     3121 cccgcacggt ggtggtgagg gcaaggctcc aatcggtgga cagccgaaga ccaagtgggg
     3181 caagcccgca ttccagcgga cgcgaaacaa caagcggaca gaatcgatga ttgttcgccg
     3241 ccgacccgcc aacaagcggc gctaaccgac gaggcgcgac cggaagtcga tctcacgccg
     3301 ccaccggtag cgcttcattt gatgggaacc caggagaaca acgtgtcgag atcttcaaag
     3361 aagggaccat acatcgaccc gaagcttcag aagaaggtcg atgtcctgaa tcagtcaaac
     3421 cagcgaacgg tgatccgcac ctgggctcgt gcttgcacga tcttcccggg aatggttggt
     3481 cacacgatcg cggtgcacaa cgggcggcag catgtgccga tctacgtcac cgagccgatg
     3541 gtgggccata aactgggaga gttcgctccg acccggacgt atcgcggaca tggcaaggat
     3601 gccgatcgcc gcgggcgccg ctagccggac ctgaacggta gaggacgata aagcaatgga
     3661 agtcagggca agcgcacagc gcgtccgcat ctcgccgcga aaggttcgac tcgtcgtgga
     3721 catggtccgc ggcaagaaag tctcggacgc catccagatg atgaagttcg tgccgaatcg
     3781 cgcggctgtc gatgtcgaga agctgttgaa gtccgtttcg gcaaatgccg agaataatta
     3841 cgacctggat ccggacaatc tctggattaa ggccatctat gccgacgatg cgccgcagct
     3901 ccgccggttc aagccgaagg cgcgtgggcg cgtcggcaag attctgcggc gttcgtgcca
     3961 catcacggtc atcgctgaag aaaggggagg ccgctaatgg gtcgcaaagt taatcccgta
     4021 ggcttccgtc ttggcattgc cagcgactgg gagtcgaagt ggttcgcgga aaagcagtat
     4081 actgagctct tgcacgagga tctcgcgatc cgaaagctcg tcatgaatga gctgacccgt
     4141 gccggtatcg ctcgcatcga gcttgagcga gccgccaaca aggtcgatgt cacgctgcac
     4201 acggcgaagc cgggcatcgt catcggcaag caaggtgcca acgtcgagcg cctgcggcag
     4261 ctgatcgaga agaatgtcgc caagaaggtc aacattcgga tcgaagagat caaggtcccg
     4321 gagaccaatg cacggctcat cgccgagagc atctccgagc agatccagcg ccgtgtggcg
     4381 taccgtcgtg caatgaagca tgccattcag caggcgatgc gacgtggcgc caagggcgtc
     4441 aaggtgcgcc tgtccggccg ccttggtgga gccgagatga gccgcaccgt gagcgagatg
     4501 gaaggccgtg tgccgctgca cacgcttcgc gccaacatcg aattcgcgac cgtccacgca
     4561 catacgacct acggccgcat cggcgtcaag gtttgggtgt acaagggcga cgtgatgccg
     4621 cagaaggccg aaattacgga agcgtcgctg accgccgatc tcgcaaaggc gggcgattaa
     4681 ttcagtcttc cgcgttgcgg ggtcggttgc cggccgccgc aacgtgacgt gaagcggagt
     4741 ctactgacat gttgatgcca aagcggacga agtaccgcaa agtaatgcgc ggccgcatga
     4801 cgggcaaggc ctatcgcggc tcgacgatct cgttcggcga tatcgccctc cagtcgattg
     4861 agcccgcctg ggtaagcagc cggcagatcg aggcctcccg tcgtgcgatc acaaactact
     4921 tgaagcgcgg tggcaaggtc tggatccgaa ttttcccgga caagtccgtc acccagaagc
     4981 cggctgagac ccgaatgggc tcgggcaagg gcagcccgga gtactgggta gccgtcgtca
     5041 agcccggccg catcctcttc gaggtcggcg gtgtggacaa tgagcgagcc tccgaagcgc
     5101 tacgccgggc agcgatgaag atgccgatga agtgcaaggt catcatccgg gaaaccccgg
     5161 gtgcgcaaac gaccgaggaa ggtgtcgcat gaagccgagt gagcttcgag gcatgacaga
     5221 ggagcagctt gaggcccgcc tcaaggagct ccatacggaa tggcgtgacc tgcggttcca
     5281 ggatgcggtc gggaacctga ccgcgacctc ccgcatccgc gagatccgca aggacatcgc
     5341 gcgtatccac accattcgta ccgagcaggc aaaccaggct gtcagccagt gacctggatt
     5401 gaggattaga gggaaccaat gagtgaggtt caggatgctg cgactccgat cgaagctgct
     5461 gtcaaccggt tgcaaaaacc gcggacgcag aaggtcggtc gagttgtgag cgacaagatg
     5521 gacaaaaccg tggtcgtcgc ggtggactat gtgcgccggc acccgctgta tcacaagcgg
     5581 attacccgga cgagcaagtt cttcgcacac gacgagagcg gtaactgcaa gcctggagac
     5641 ctggtccgca ttgaagaatg ccggccaatg tccaagcgga agcgctggat cgttcgcgag
     5701 gttgtcgagc gcgcggttca gatctgaccc gccgcggcgg cgaacccgtc gcgttcggtc
     5761 gatgcccaga gagggtaggc agggatgatt cagacacaga cccggttgaa ggtcgctgac
     5821 aatagcggtg cacgtgaaat catgtgtatc cgtgtgttgg gtggctcctc gccgaaattt
     5881 ggaagcgttg gagataccat catcgcttcc gtcaaggccg cgcaaccgaa tgcagccgtg
     5941 aagaagggcg acgtcgtccg cgctgttatc gttcgcaccg cgagcgagta tggccggccg
     6001 gatgggagcc acatcaagtt cgatgacaac gccgcggtgc tgatcaatca gcagggtgct
     6061 ccgcgtggga cgcgcatctt tggcccggtg ggccgggagt tgcgagagcg ccagtacatg
     6121 cggattgtct ctctagcacc tgaagtgctg tagggatcaa tgatgaggaa gattctcgca
     6181 ggcgatgagg tactcgtcat ctccggtcgc aacaagggct cgcgcggcgt cgttcgcaag
     6241 aacctgacgg atgtcgatcg tgtggtggtt gagggcgtca atatcgtcaa gaagcacatc
     6301 aagcgtggac gcgcgcggca ggcgggcatc gtcgaggtcg aggcgccgtt ccatgtatcg
     6361 aacgtcaagg tcgtgtgtcc ctcgtgcggt gaacctacac gcataggcgt gcgggccaat
     6421 gacgagggca agaacgagcg tcactgcaag aactgtgatg ccgtggtagc gcagcagtcc
     6481 cgctaggcag gtttgggcgc gacaggcagt attggaacca taagaggatt ggcgacctga
     6541 gtgctgaact cgaatcagcg gaccaggtgg gtaacgccac gcgattggta ttgggatacc
     6601 ggacctttat ccggtgtgag gcagggttga atggcacggc tacatgatcg ctacaaggag
     6661 gacatagctc cggccttgac gagcgagttc gggtacaaga acccctttca ggtgccgaag
     6721 ctggagaaga tcgttctgaa tatcggtctc ggtgaggccg ttgcgaacgg ccgcgcactt
     6781 gatgccgcgg tgaacgatct caccacaatc accggccaga agccggtggt gacgcgagcc
     6841 aagaagtcaa tcgcgtcgtt caagcttcgg gaaggcatgc cgatcggcgc gatggtcacc
     6901 cttcgcggcg cgcggatgta tgagttcatg gaccggttgg tagcgactgc gcttccgcgt
     6961 attcgtgact tccgcggcat ttcgccgaat gccttcgacg gtcgcggtaa ctacacactc
     7021 ggtcttcggg agcagatcat gttcccggaa atcgactacg accagattga taaagtgcgc
     7081 ggcctcgaga tcagcattgt gacctccgcc cgtactgatg aagaggggcg ccggttgctc
     7141 gcgcttctcg gaatgccgtt cgcgcggtag cgcggaacca cggaggaatc atggcgaaga
     7201 agagttcgat cgcaagggcg gcccgcccgc ccaagtactc gacgcgctcg gtgaatcgat
     7261 gcaggctctg cggccgcagc cgtgcctata tgcggaagtt cggcgtctgc cgtatctgct
     7321 tccgcgagct ggcttccctc ggacgacttc ctggcgtaac gaagtccagc tggtaatcga
     7381 gtcacccgcg tgctgaacgc gcacggtaga gagcgacgga gaagagaacg catgagcgta
     7441 aatgacccga tcagcgacat gctgacgagg atccgaaatg cggggatggc gcagaagcgc
     7501 gaaacgagca tgcccgctac caagatcctg atagccatcg ccggcatcct gaagagcgaa
     7561 ggctacatcg aggactttca ggtaattgaa aagcgccccc aggaccagct ggtgctgaca
     7621 ctgagctacg gtcctgacaa gcgccacacg attcgagaga tcaagcgcat cagcaagcca
     7681 ggcctgcggg tgtacgccgg caagagcgag cttccgcggg tcaagagtgg tctcgggatc
     7741 gcaatcgtca gcacaccgga tggcgtgctg acggggtacg aagcccggcg tcgcggaatt
     7801 ggcggcgagg tgctctgcac cgtctggtaa gccaatatgc cggagatcac tcccgcgata
     7861 cccggcgcac gacctgattt gaggaagagc catgtcgaga attggaattc agcccattgt
     7921 ggtgccgaac ggcgtgaacg tcgagatcgg tagcgacaat gcggtacagg tgagggggcc
     7981 caagggccag ctgagccaga agttgacgcc gcgccttcag atcgtccagg aggacggatc
     8041 tgttcgcgtg gttcgcacca gtgaggagcg cgaggtgaag gccctgcacg ggctcacccg
     8101 ctcgctcctg aacaacatgg tcgttggtgt gaccgaaggc tacaccaaga ccttggagat
     8161 ccagggagtg gggtaccgcg cgcagatgca ggggaagact cttgtgctga acgtgggctt
     8221 ctcccatacg gtgccgatcg ttccccctga gggaattacc ttccaggtag aaggtaataa
     8281 ccgtgtcctg gtcagcggca tcgacaagca ggctgtgggc gaagaggcgg cccgcattcg
     8341 tggcgtccgg cctcccgagc cgtataaggg caaggggatt cggtacgcag gcgagtttgt
     8401 ccgccgcaag gccggcaagg ctggtaaggc caagtaatta accggcaggg taatcctgta
     8461 gtcgctgcga cggccgccaa tgcatggtgg gccaacgcgg tgccggtccg aacggagtta
     8521 gcacatgcgt ttgaagtaca gaagggcact gagccagcgc gagcgccgtc acaagcgcgt
     8581 gcgcgccaaa gtgaatggga ccgcggcccg gccgcgactt aacgtctacc gctcctcggc
     8641 tcacatatat tgccagatca tcgatgatgt gaagggccac acgcttgtcg ccgcaagtga
     8701 tctcgaagac gatgtccggg agcgggctgg cgacgacgct gccaaagtgg cgagggccag
     8761 ggccgttggg gaagtaattg cccagcgagc cagggaagcc ggcgtcgacg ccgttgtttt
     8821 cgatcggggt ggattcctct atcacggtcg aatcaaggcg gttgctgacg gcgcgcgcga
     8881 ggctggcctg aagttttagg ccgccactat ttccggtgcg taaggcagaa ggccaagtgc
     8941 cggaagatca ggagatatcc atggatcgta atcgggaaga gcgaggttcc cgccgtcgca
     9001 atgacgacga gcaggcgaac gagcttgagg agcggttggt aaaaatcaac cgcgtctcga
     9061 aggtacacaa gggcggacga accttcgcgt tcagcagtat cgtcgtcgtc ggtgacggca
     9121 agggccgcgt cggtgtcggt atcggcaagg ctggcgaagt accggacagc atccgcaagg
     9181 gcggcgagtc tgcgaagaag ctgatgatca aggttccgct ggacggcgga acgattcccc
     9241 atgaggtcga atcgaccttc ggtgcgtctc gcgtgctcct gaagccggcg tcgcctggta
     9301 ccggggttat tgccggtggc gctgtccgcg ccgtggttga agcggcgggc atccgcgaca
     9361 ttctctccaa gtcattgggt agcaataacc cggtgaacgt cgtgcgtgct accgttcagg
     9421 ctctggagga gctggagtcg gagaacgcgg tgattcgccg ccgtaatcgg cagaaggcga
     9481 cgttgcgcaa gccgttcgag catggcggac agtcggccga ggccgtgccg accaagcgac
     9541 ggcagccaga accgcttgtt ccacctcagg aaggtggacg acgtggaggt ccgggcggcg
     9601 gacgcggacg cggccagcag ggcggcggcc gtggtcgtgg tcagcagggc cagcaaggtc
     9661 agcagggttc tgcacagagg ggacggtaag ccatgagcga tacgcaatcg actggcaatg
     9721 gtcagctgcg gatcacactg gtgcgcagca ccatcggacg ccccggcgat caggagcgag
     9781 cggtgaaatc gctcggtctg cgcaaggtga atcacaccgt ggtgcgcaac gacgatcctt
     9841 cgatccgggg aacggtaaac aagatcagcc atctcgtgag ggtggaagaa gtcgccgccg
     9901 agtaaccctt acgtgggttg aacggccggc atgaaggttg ggaacaacga tgcccctgac
     9961 aatagatgat ttgcggccaa acaagggcgc aaacaccagc aagcgccgcc tcggtcgcgg
    10021 ccatggcagt ggccgtggca agacggccgg acgtggcacc aagggccaga actcccgctc
    10081 cggcggtggt gttcggccgg gattcgaagg cggtcagctg ccaattcagc agcgcatgcc
    10141 ttacaagcgt ggcttcacaa acgtctggcg cacgccgtgg gaaacggtca atgttgggcg
    10201 tctcgcggag cttcaaatcg atggaccaat cacccctcag gtgcttgccg agcgtggctt
    10261 gatccgcagc gtccggtttc ccgtgaagat tctcgggaat ggtgagctcg gcacggccat
    10321 caccgtccac gcccacggct tctccaaatc cgcaaaggag ctcatcgagc aggccggcgg
    10381 aaccgtggag acgcttgagc gaacggatca gtggacgagc gcccgaccgc ggacccgacg
    10441 tctgccaatc aaccgcgaga tgaaggcgat gcgcgtcggc aaggtcggtg gaccgagccg
    10501 tcgtgaggca ttggcccaaa tcggggaacg cgggggcgag gaggcctagg catgctccag
    10561 gccgtgatca acgcgttcag gattcctgac ctccgtcgga agatcctgtt taccatagcg
    10621 atgctggtgg tctttcgatt ggtcgccagc gtcccgatcc ccggagtgga tcgccagggt
    10681 cttcgcgact ttatcgagac aaaccagctt ctcgggatgc tgaacctgtt ttccggcggc
    10741 ggtctgaaca acttctcgat cgcagcgctc ggcgtgtatc cctacatcac ggccaccatc
    10801 atcatgcagc tcatgacgcc gattattccg cggctcaatg agttgtctat ggaagggcag
    10861 cagggccgta accagataga tcgctacacg cattatctaa ccgtgcccct ggccctgctt
    10921 cagggctacg gtcagatgct cctgttcgca agtgaccaga gcgggaacct cgtccagaac
    10981 ttcggtttgt tcgatgcgga tacattcctg ccgacgctcg cgattctgct caccctgacc
    11041 gcgggaacga tgttactcgt ctggatcggc gagctcatca ccgagaacgg tatcggcaat
    11101 ggcgtgtcga tcatcatctt cggcggcatc atcgcttcac ttccgagcgc gataggcgga
    11161 ttggtgtcgg gtggaacact aggtgaaaac gtagcgggga cggtcttctt cgttgcgatc
    11221 tcgctactca caatcgtggg tgtcgtgctc atcaatgagg gacagcgacg aatccctgtg
    11281 catcatgcca agcgcgtgcg ggccggtcgg cagtatggcg gcaacacgac caatatcccg
    11341 ctgaaggtca acagcgcggg tatgattccg ctgatcttcg cggtcagcat catggtcttt
    11401 ccggggatgg tcgcgaactt ccttcagtcg tctggtacag attgggtgcg aagcctcgct
    11461 gtcaacatca gccagtggct gagcccaaat actgtgcctt acaacatcgt ctacttcttc
    11521 atggttgttg gattcacgtt cttctacacg atggtcgtat tccagcagca gcggattccg
    11581 gactcgttgc agcgtcaggg cgcgttcatc cccggtatca ggccgggtcg caacaccgca
    11641 atctacctgc agagggtgct cactcgcatc acgatcgtcg gcgcgctctt cctgggagtc
    11701 atcgctatcc tgccgtttat cgcggcacgg ataacaggtg tccagaacct gttgctcagc
    11761 tcgacatcct tcctgatcgt cgtcggtgtc gcgatcgata caatgcggca gcttgaggcc
    11821 cagctgatga tgcgaaacta cgaaggcttc attgactagg cgagcatacc ggtaggtcac
    11881 tcaacgccct gccggtgtgt tggtatcccg cggggaagga catgcccttg tgtatgtgat
    11941 tctgatggga gcccaaggct ccggtaaagg cacccaagcc gctctgatcg gccccaagct
    12001 gaagctcgtc aaggtcgcca cgggcgacct cttccgtgca gcgatcgccc agaaatcaga
    12061 gctcggactg caggtccagg ccattctgga gtcgggcaat ctcgttcctg atgaactgac
    12121 caacgcgatc gttcaggttc gcctcgcgca gattgcggca gcaaaggcga agggtgagga
    12181 cgtcgctggc gcgctcttcg acggatttcc gaggactgag ccgcaagcgc gggcgcttga
    12241 cgagatcctg gcgcaccagg gtgagtcact gaccgccgtg gtcgaaatcg atgtaccgcg
    12301 cgagaagctg gtcgcgcgat tggctggccg gcgcgtgtgc gagtcctgcg gcaccgttta
    12361 ccatgtggaa gcagacccac cggtagtgcc ggggatttgc aataaatgtg gcggcaagct
    12421 cctgcaacgg gaggacgaca cgccggaggc aatcacccgc cgcctggagt tgtacgacga
    12481 tctcacacgg ccgctgcttg cgtactacgc tgagcagggc gtcctcgagc gcgtggacgg
    12541 ggaccaggat atccagaagg tcgagcaaga ccttctggac gccatccaga gccgcgccga
    12601 atcaatgaaa gcctagacga gccatgccga ttacgatcaa gaacgagcga gagattgaga
    12661 agatgcgagc cgccggccaa gtggttgcgc tgtgccacca gagagtggaa gccgccatcg
    12721 cgccaggagt aacaaccgcg gatctcgacg cgctcgttcg cgataccgta aaggagcacg
    12781 gcgcaatcgc cagttttctg gggtacaacg ggttccgggg aaatatctgc gcctctgtaa
    12841 atgaagagat cgttcacggc ataccgggat cgcgcgtcct gggcgacggc gacgtcatcg
    12901 ccgtggatat cggcgcgatc ctcgacgggt ggcatggcga ttccgcctgg acctatcccg
    12961 tcggtgaggt ctccgacgac gcaaggagac tccttgctga tacggaagag gccctgaagg
    13021 taggagtggc ggcagctcgg gcgggtaacc gacttggtga gattggacac gcgatagaag
    13081 cctacgccgc tccgcgcggc tacggcatcg tccggggcta cggcggtcac ggcatcggca
    13141 ggcgaatgca cgaggaccct catgttccaa actacggcga ccgcacgtac ggccccgtgc
    13201 tccgtaatgg catgacgctg gcgattgagc caatgttgac gctgggcacc gacgaaacac
    13261 gcgagttgga agatggctgg acggtcatca ccgccgatgg aacgctctcc gcgcatttcg
    13321 agcacaccgt ggcgatcacc gacgaagaac cgattgtctt gacaaaacgg ctggtttcag
    13381 tggtaaaata gtcattcgtg ggcgcatgtc ggtggacatg cgctatccgc gtgctcgcca
    13441 ttggccctga tcctccttgt aagccaaact cggaagatcg gaggctgcgt gcaatgcatc
    13501 ggcagccggc gagggatgac ccgtggtcgc ccgggagata cgtcgaagaa cactctcacg
    13561 ttcctggctc tcgcgcgaaa aacgaagcag accccatgcg cactgtatgg atatggaatg
    13621 aggagttggt ttcgatgaag gtttcggctt cggttagtaa gcggtgtgat cgctgccgca
    13681 tcatccgccg caagggcatc gtgcgcgtta tttgcacgaa cccacggcac aaacagcgac
    13741 agggctaatt gaaccgtagt ccggagtacc agcagcgctg ggattgatat cggactgcag
    13801 atattgaggg gaaagcatgg ccagaattgc gggtgtcgac ctgccgcgtg agaagcgcgt
    13861 tgaggtgggc ctgacctaca tctatggagt tggtctgcat acgagccaaa ggttgctcga
    13921 gcagaccggg atcaatcctg atacacgcgt ccgcgatctc accgacgacg aggcaaaccg
    13981 gcttcgtgat gtgattgacc gggaagtacg tgtcgagggt gacctgcgcc gcgaaatctc
    14041 catgaatatc aagcgtttga tggatattgg ctcgtatcgt ggtgttcggc accgccgcgg
    14101 cctgccactg cgtggacagc ggacgcgcac gaatgcgcgc acgcgccgtg gtccgaagcg
    14161 ggcaattggt ggacgcaaga agtaattggt cggaagcgat caggtggaga tgggcagggc
    14221 cccgtcaagg atctctgatc gtgaccgtgg aatctgtagt ttcgaagtat taggtggagg
    14281 cattatccgg catgtcagag cgacggcgcg gtacagctaa aagtcgagtt cgaagacgcg
    14341 acaggaagaa tattccccga gggaaggcgt acatccaggc cacgttcaat aatacgattg
    14401 tgacgctgac cgacccgaac ggcaacgtga ttgcatggtc cagcgcaggc gccaatggct
    14461 tcaagggatc tcgtaagagc accccgttcg cggcgcagat gacggctgaa gccgccgccc
    14521 gccgcgcgca agagcatggt cttcgccagg tcgatgtgta tgtccggggc cccggctctg
    14581 gccgggagat ggcagtccgt tcgttgcagg cgagtggtgt gcaggtgatc tcgattaccg
    14641 ataccacgcc gatcccccat aacggatgtc gtcccccgaa gcgccgccgc gtctaacgac
    14701 aacccggcgc atacatcaca cattgatagc cagtcaggta tcaaagggat acatctggct
    14761 ggccgcaccc tctcaccacc aagtagctgg tgagcgggtg acgcgaggag gcgagtccgc
    14821 ttgttgcagc aacccgaatt tacaatcaag acagtcgagg cggggcgcaa ctatgggcgc
    14881 tatcaagttg agccgctcga gcccggttac ggaaccacgc taggcaactc cctgcgccgc
    14941 attcttctgc ggtcgctcca gggctgtgca gtgtcgcgaa tccggatcga caacgtttgg
    15001 cacgagtttg ccaccgtccc caacgtccgc gaggacgtca cggagatcgt gctcaacctc
    15061 aagtcggtcc gcctgcgtcg cgtgaccgag ctccgcggtg acgtgcgtgc gcatctctat
    15121 gcacgtggcg aaggcgacgg cgagcgggtc gtcactgctg gagacgttga gtggccagtt
    15181 gaggttgatc tggtcaaccc cgatcagccg attgcgacgc tcgatgggcc ggatgccgta
    15241 ctcgacatgg atctctggat cagccggggc cgtggctacc gcccggccga agcccaggag
    15301 actgtcgcac tcggtgagat cccgatcgat tcgatcttta cgccgatcga acgcgtgaac
    15361 tatgtcatcg agcagacccg cgtagggtct gaagttgact acgatcggct gatcctggag
    15421 atcctgaccg acgggacgat cgatcccgac gacgctctgt cacagtccgc caagatcctc
    15481 atggatcatg cgcgggtctt tgcagacttc acccggccag aaccagaaat agcggcgaac
    15541 tccttcatct ctgaggaggt gcagaacaag ccgctcgcag atcttggcct gtccccgcgg
    15601 gtgctcaatg cgctgcggag caagcagatc gacaaggtcg gccagatatt gacgatggat
    15661 ccggatcagc tgttgtcgat ccggaacttc ggcccgcgtt cgcttaccga gctgcgcgaa
    15721 aagctgaccg aatttggata cctctccgat gtgccggcga tcgacctcgc tgcctacggc
    15781 gaggacgata gtgaagcaga cttcgtggat gctctggcgc aggtcgctga agcatccgcg
    15841 gttgatgagg attcgaccaa tgaggcaccg taagcaaggc cgaaagttcg gccgcaaccc
    15901 ggcgcagcga aaggcactgc ttcgacagct ggcgatctcg ctcatcctga atgagcgcat
    15961 caccacgacc gaagccaagg ccaaggagct gcgaagcgtc gtcgagaagc ttgtgaccat
    16021 cgcccgtgag gactcgtccc acagccgaaa tatcgtcatg tcgcggcttg atcacgagcc
    16081 atccgtcaac cggctgttcg atgaggttgc tccgcgcttc gagggcacac caggcggcta
    16141 tacgcgtatg gcaaaactag gcgtgcgcaa gggcgatgcg gcaccgatgg tcctgatcga
    16201 gttcgtgagt tagttcgtac tgcgggcagc ctggttgttg agatgcggcc cgcacattgt
    16261 tcctgacgca aggcacgtat ggccgctacc ctgaagttca cgcttgcata cgacggcacc
    16321 ggcttccggg gttcacagcg gcagaagaac gggcgaagcg tacaggagga tctggagacc
    16381 gcgctctcca gattgaacgg tcgggcgacg caggttgtct tggccggccg gacagattcg
    16441 ggtgtccatg ccgtgggcca ggtggccagt atcgaggacg ttcgcccgga tctgcccgat
    16501 caccggatgg tgcaagcgat caatgctcat ctggccgagg atcttgcggt cctgggttgt
    16561 gagcggagag aagg
//
LOCUS       DASYWM010000248        12175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_29596, whole genome shotgun sequence.
ACCESSION   DASYWM010000248 DASYWM010000000
VERSION     DASYWM010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12175
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29596"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..609
                     /locus_tag="VGR29_10175"
     CDS             94..609
                     /locus_tag="VGR29_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV2073994.1"
                     /translation="MSETQKATVSTTVNGEPKTVAVEPRTLLVHMLRDELRLTGTHIG
                     CETGQCGACTVLLDGKAVKSCMVLAVQAEGHHVTTIEGIAPPGQLHPIQQAFWEEHGL
                     QCGYCTPGMILSSLALLSEHPDPDDETVRHGIEGNICRCTGYNSIVKSVQSAAERIGR
                     GDKPQPIQSGD"
     gene            674..1534
                     /locus_tag="VGR29_10180"
     CDS             674..1534
                     /locus_tag="VGR29_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEV2073995.1"
                     /translation="MHPYPFAYRRASTVNEALALLASEEEPKLLAGGQSLLPVMKLRL
                     AQPGTLIDISEIADLRGVRLDGDTLVIGALTTHHDLETDPLIATHAPLLAEAARVVGD
                     QQVRNRGTIGGVLAHADPAADYPAGVLALEATVTATGPNGEREIPVTGFFQGFMTSAL
                     AENELLTSIRIPILDGARGTSYQKLANPASGYAIVGVAAVVTLTADGSVGSLKLGLTG
                     VGDVAYRATAVEEALAGQQPTAEAVKEAASHAVDGIEPLGDLHAPAAYRGKVARNLSR
                     RAIMIAVERA"
     gene            complement(1705..2205)
                     /locus_tag="VGR29_10185"
     CDS             complement(1705..2205)
                     /locus_tag="VGR29_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2073996.1"
                     /translation="MRGQDLPEGVVPGRRFPETPHELPYDRAKVDALLERVRKGEKVR
                     LLDELLACVDWRGAFGADDGQPIDLGHISKLIAYYREKFSDIGSVYMAELLSTEFITE
                     QRARGTIVFSEKLLELGRSDPELWKEIRLFFRRKEFATAMLVHANEDVSDDEMDARQS
                     QWTPER"
     gene            2593..5073
                     /locus_tag="VGR29_10190"
     CDS             2593..5073
                     /locus_tag="VGR29_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV2073997.1"
                     /translation="MSQGRIGRSTRRYPGTLRRSAHFSHSRYGHRGRGLPPHLMPGVS
                     RQKKEEGVFSRRNFLLGGVAVFFVTLLSFLSITVVSAVSGAMGTVSAYRKVNRELPDA
                     ARIAVNTFQTTRIFDRKGTLLQEVDNPDYGWRTFIPMGQMAETFVNASVSAEDSTFWT
                     NQGVEPFAILRGGLIIASGSGSSGGSTITQQLVRAVFPEQISAMDMSLTRKFREALAA
                     VALAQEYSKEDIFTMYVNQIYYGARSYGVEAAANTYFSKHASELTLAESALLAGLPQA
                     PSYYDPTTPEGFEQAKIRQGYVLDQMRKYGYITRAQADEAWAEPLQIRESTRTNAIKA
                     APHFTQYIHGYIFDTFGEDAVYSGLDIYTTIDLDLQRAAEEILVQGVANMQQYQRNNG
                     ALVVICPWNGEILAMVGSADFNNAAISGQVNFATSLIQPGSSMKPIAYAAAFEKGWNP
                     GTVIMDIPTTWDVPGQEPYEPNNYTKQFYGAVHAREALANSFNITAVKATDFAGVEDV
                     MDMARRMGIVDSLENDAGFYGLSLGLGAGELQLLEHTNAYATLANNGRYVPANPIQRI
                     QDSQGNLLFEVTPESIEKEAVQAMNPGNAYQVTSILTDNDARARVFTRDNRFGDTQSA
                     LGRPTAAKSGTTENWRDLWTMGYTSAAAVGVWVGRSGDAGTAQLPEIDGIQAAGPIWQ
                     DVMMLIHETPEFAALLNGPDGQPVPGDFPVPDEAYETDLCAVTGHRPGSGDTIEDWVV
                     RDQEPTLACTEVNEYEAEQLADALEALREGANWANGAAESINRYARMVGRSAVPIEGD
                     DEESEESDDSGETGGTDDEEQPPIEPLD"
     gene            complement(5194..5787)
                     /locus_tag="VGR29_10195"
     CDS             complement(5194..5787)
                     /locus_tag="VGR29_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEV2073998.1"
                     /translation="MSNLDTLDERILSELQSNGRLTMKALAERVGLSSPAMIERVRRL
                     EERGVLAGYRAIVDPHAIGRSLTALISVDLDRRHYDAFLNRLRSEPAVEECHRVTGEH
                     AFIVKAHVASTEDLESLIDELQDVGAKCSSNIVLSTPIVDAPIVPPEGSVSERTRLTR
                     RRRRAAIESAVKGEGAPRRRGRPKAAATASQKEETSE"
     gene            complement(6051..6791)
                     /locus_tag="VGR29_10200"
     CDS             complement(6051..6791)
                     /locus_tag="VGR29_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEV2073999.1"
                     /translation="MTTESPKCLVVGWLYGTKMNIYGDRGNVLAIAQRARWRDIDVEI
                     REIGFGEPIPDDVDIFFWGGGQDQEQVAVSHDMQGEKAEQLRTAIENGAAMLAVCGGY
                     QLLGHEYRPHNGEPLRGVGLFDAASTAGPERFIGNVVVNSRWGTLVGFENHSGLTWLG
                     EGVEPLGKIQVGRGNNGQDGTEGAIYRNAIGCYLHGALLPKNPALTDWLIEMGLRHRY
                     GSADLPDIDDDLERRAHETAMQRAFTTR"
     gene            complement(6845..8275)
                     /locus_tag="VGR29_10205"
     CDS             complement(6845..8275)
                     /locus_tag="VGR29_10205"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEV2074000.1"
                     /translation="MDKEFRTAQGRDLRLVASLATAKVAGAVIRRLGRGGGTSAPGLV
                     ADRLDSAMLTKLAAGLEQGAVIVAGTNGKTTTTRMIADVFELVPWRVVHNRSGSNLVR
                     GAIAAFVQRASLTGCPGGEIAVIEADEAALQELVRRVRPRIVLLNNLFRDQLDRYGEL
                     DTIGKTWRGVLKDLDAGTTVIVNGDDPALVVISDSITAKRICFGLDEQTHRLDVLPHA
                     ADAAVCRVCGADLAYHALYVSHLGDWYCPNCGRKRPDLTYTGSNIELQGVQSLALNIS
                     EGGGHTHHLTVGVPGLYNAYNALAAAAVAREVGIPWELIGQAFDAFRSAFGRIERVQY
                     KGRDLTLALVKNPTGFNEVLRMLTAETGGLALPTMIAINDLDADGRDVSWLWDVDFEL
                     LARGKGDLYTTGLRGPDMANRLKYAGVALDRIHSLPNELPAGLDAFVEAVPEGHAAYM
                     LPSYTAMLALHKTLADRGAVDAFWEQ"
     gene            complement(8268..9080)
                     /gene="mreC"
                     /locus_tag="VGR29_10210"
     CDS             complement(8268..9080)
                     /gene="mreC"
                     /locus_tag="VGR29_10210"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV2074001.1"
                     /translation="MTNVPMRRVVSLVLIFIVISAIFLVLDRRSLLDPIRDGLGEVLA
                     PIASTFEGVVQPRVGGSDLEVAYATVVADRNRVVAENARLKAEVEELKALREQVRVEQ
                     LRPDITYVPARVTGQDPTGSQQFITINKGSADGLRQGMAAVSPDIFVGQITEVQEHQA
                     RVLLVTDPALAVGAMLYDARADGVVYGTRGSGGLLIMRHVDKDVVPSELEWVVTSDVA
                     ASETAQVPSNIPIGVVVGEPALNAQSDQLEIMVQPAADFENLMTVWIAVPNG"
     gene            complement(9158..10213)
                     /locus_tag="VGR29_10215"
     CDS             complement(9158..10213)
                     /locus_tag="VGR29_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEV2074002.1"
                     /translation="MFRPLSTLFGFFSRDLGIDLGTANTLVYVRGKGIVISEPSVVAI
                     DTRTKRALWVGMEAKAMIGKTPESIIAVRPLEDGVIADFDTVEMMLHHFIRKVHTQRL
                     MAPHPRVVIGIPSGVTEVEKRAAKDAALSAGAREAYTIEEPMAAAIGAGLPINEPIGS
                     MIVDIGGGTTEIAVISLGGIVVNHSIRVAGDEIDDALIQYARRDHNLVIGERTAENAK
                     IAAGSAYPLEEEVRVMLRGRDLLTGLPKSVEVSSVELRDAISQPVNTIIELVKTSIEE
                     TPPELIADIMEHGITLAGGGALLMGLDRRLQSELRMPVHRADDPLTCVARGTGRVVES
                     LHLYQRALAGGQELRRG"
     gene            complement(10527..10991)
                     /locus_tag="VGR29_10220"
     CDS             complement(10527..10991)
                     /locus_tag="VGR29_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003913551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV2074003.1"
                     /translation="MHTSSPMTVDTGDRQVARSIVVNAPVDELFALVANPHRHHELDG
                     SGTVRDQAEGPERLNEGDRFTVHMRIYGVPYMITSTVTRAEPGKVVEWRHPAGHRWRY
                     EFEPLGPSETRVTETFDYRDCKVARVYEMLGVPEKNRSGIERTLEKLAHRYA"
     gene            11104..11913
                     /locus_tag="VGR29_10225"
     CDS             11104..11913
                     /locus_tag="VGR29_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2074004.1"
                     /translation="MVRKQAQEDGWATIRDVLSSRPDGTEVGLSVYLFGSGQTLSIDG
                     ERDFASASTIKILILAALARGFDEGRFSPEDKIAVREDLKLEGSGVLNWLETGLRLSL
                     HDHAWLMTAISDNTASNICIETVGLDEINALGNEIGVGKTHLGRMFMGANAPPGPPKN
                     RATADGLVAILKAIENDTAASPERCAWMRSCLDDQQHVDRLPRHLPEGISYRGKTGTI
                     QGIAHDCGALTGPGGRIALAVLTEGFRNPYDADRLIGRIGAAAASLVAGND"
     gene            complement(11928..>12175)
                     /locus_tag="VGR29_10230"
     CDS             complement(11928..>12175)
                     /locus_tag="VGR29_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074005.1"
                     /translation="SDIAGARDGVSDRIRVSFHQGDQQYDLLFDFDVPAPGLVGKEQR
                     TRFLNALAACGVPVSTAAAATAPWRHRGEVHQGRSWQ"
BASE COUNT         2479 a         3860 c         3584 g         2252 t
ORIGIN      
        1 cagccagcgg cgaagatgat cgtcggcaag ttcttcgaat cactggaaac taccgtcacg
       61 cgctagatca caaaggcaac tggaggaaac ccggtgtcag aaacgcagaa ggcgactgtc
      121 tccacgacgg tcaacggcga acccaaaacc gtcgcggtcg agccccgtac cttgctcgtg
      181 catatgctgc gggatgagct gcggctaacc ggtacgcata tcggctgcga gacgggccag
      241 tgcggagcct gcactgtcct gcttgacggc aaggccgtca agagctgcat ggtgctcgcc
      301 gttcaggccg agggccatca cgtcaccacc atcgagggca ttgcccctcc cggccagctt
      361 catccgatcc agcaagcctt ttgggaggag catggcctcc agtgtggcta ttgcactccg
      421 ggcatgattc tctcatcgct ggccctgctg agtgagcatc ctgatcccga cgacgaaact
      481 gtccgccatg gcatcgaggg gaacatctgc cgctgtaccg gctacaactc catcgtgaag
      541 tcggtccaga gcgccgccga gcggatcggc cgcggtgaca aaccacagcc aatccaatcc
      601 ggcgactagg gtccccgata ccggctttgc tgccagacga acatagccac atccacttga
      661 ggaggagatc ctgatgcacc cgtatccctt cgcgtatcgt cgagcgtcaa ccgtcaatga
      721 agcactggcg ttgctcgcct cggaggaaga gcccaagctt ctcgccggcg gccagagcct
      781 gctcccggta atgaagctcc gcctcgcaca gccaggaacg ttgatcgaca tcagcgagat
      841 cgctgacctg cggggcgttc ggctggatgg cgatacgctg gttatcggtg ccctgacaac
      901 ccaccacgat ctggagacgg acccgctgat cgcgacgcat gcgccgcttc tcgcggaggc
      961 tgcgcgggtg gtaggcgatc agcaggtgcg caaccgcggc acgattggcg gtgtactggc
     1021 gcatgctgat cccgcggcgg attatcccgc tggtgttctc gcactggaag cgacagtgac
     1081 cgcgaccggc cccaatggcg aacgggagat tccggttacg ggattcttcc agggattcat
     1141 gacctccgca ctggccgaga atgagctcct gacatcgatc aggattccga ttctggatgg
     1201 cgcgcgcggg acgagctatc agaagctcgc caatcccgcc tcgggatatg cgatcgtcgg
     1261 tgtcgccgcg gtcgtgacgc tgacggcaga cggatcggtt ggttcgctga agctcggcct
     1321 aacaggagtg ggcgatgttg cctaccgcgc aacggcagtg gaggaggcac ttgccggtca
     1381 gcaaccaacg gccgaagcag tcaaggaagc agcctcgcac gccgtcgacg gcatcgagcc
     1441 gctgggcgat ctccatgctc cggccgccta ccggggcaag gtcgccagga atctctcccg
     1501 ccgcgcgatc atgatagcag tggagcgcgc ataaatttcg ggccgctgca ccatttggcc
     1561 gttcattggg cggacactgc ttcgcaggta ccgcccctac tcgggtgaaa gcgtctcagc
     1621 aggtgttggg gcccccgtag cgtgtccgcc cgatgacggg cagcggcatc accggcttga
     1681 acggaggcag ttcgcgccgg ccatttacct ctccggtgtc cactgggact ggcgagcgtc
     1741 catctcgtcg tcagacacgt cctcgttggc atgaaccagc atcgcggtcg caaactcctt
     1801 gcgccggaag aagagccgga tctccttcca gagctcgggg tcgcttcgac caagctccag
     1861 caacttctcc gagaagacga tcgtgccgcg agcgcgctgc tcggtgatga actcggttga
     1921 caacagctct gccatgtaga cagagccgat gtcgctgaac ttttcgcgat agtaggcaat
     1981 cagcttcgag atatgcccga ggtcgatcgg ctggccgtca tccgcaccga acgcaccccg
     2041 ccagtcgaca caggccagta actcatccag caaccggacc ttctcgccct tacggacgcg
     2101 ttcaagcagt gcatcgacct ttgcccggtc atagggaagc tcgtgggggg tctcgggaaa
     2161 tcgacggcca gggaccacgc cttccggcag atcctgtcct cgcatttgtt catcatcctg
     2221 catggtcatg gttcaccagg gaatacaacg ggggagccgt cgtcgacacg tctcatccaa
     2281 tggtagccgg aaggacgcct ttgggcggtc cggtagcctg cgccgcagca ctgaggctgg
     2341 taaaatagca agaccacgag cgttattggc cgtttcgagg tgcgaagacg aaacggatgc
     2401 cgcggctggt acgtcctgat atatgcagga taccgccgcg atgcccttcc tctcatcagc
     2461 cgagcacatc cacaccattc cacatctcac cagggaatat cacgccaaca ccgaaagaca
     2521 cttccagacc gaacaccagc cgagccgccg ttcgacaccg aagcggaatc tgataccagg
     2581 gagaactcac gcatgagtca agggcgcatt ggtcgctcca ctcgtcgata ccccggcacg
     2641 ctgcgccggt cagctcactt cagccactca cgctacgggc atcgtggacg cggcctgccg
     2701 ccccatctca tgccgggcgt cagccggcag aagaaggagg aaggcgtctt ctcacgccgc
     2761 aacttcctgc ttggtggtgt tgccgtcttc tttgtcacgt tgctctcgtt tctctcgata
     2821 acggtggtct ccgcggtctc cggtgccatg ggcaccgtat cggcctaccg caaggtcaac
     2881 agggagctgc cggacgctgc ccgcatcgcc gtcaatacct tccaaacaac ccgcatcttc
     2941 gaccgcaagg gcaccctgct ccaggaagtc gataacccgg attacggatg gcgcacgttc
     3001 atccccatgg gtcaaatggc ggagaccttc gtcaatgcct ccgtttccgc tgaggactcc
     3061 acgttctgga ccaaccaggg ggtagagccc ttcgcgatcc tgcgtggtgg gctcatcatt
     3121 gcctcgggga gtggttcctc cggtggctcc accatcaccc agcagctggt ccgggcggtc
     3181 ttccccgagc agatcagcgc catggatatg agcctgaccc gtaagttccg ggaggccctc
     3241 gctgccgttg ccctggcgca ggagtactcc aaggaagaca tcttcacgat gtacgtgaac
     3301 cagatctact atggggcccg ttcctacggt gtcgaagcgg ctgccaatac ctacttcagc
     3361 aagcacgcct cggagctgac gctggcggag tcggcgcttc tcgccgggct tccccaggca
     3421 ccctcctact acgacccgac caccccggag gggttcgagc aggcgaagat tcgccagggg
     3481 tatgtcctgg atcagatgcg caagtacggc tacatcacgc gggcccaggc tgacgaggca
     3541 tgggccgaac cgctgcagat tcgtgagtcg acgcggacca acgcgatcaa ggcggcaccg
     3601 cacttcaccc agtacattca cggctacatc ttcgacacct tcggtgagga cgccgtctac
     3661 agtggcctgg acatctacac cacgatcgat ctcgatctcc agcgtgccgc cgaggaaatc
     3721 cttgtccagg gtgtcgccaa catgcagcaa taccagcgaa acaatggagc gctggtggtg
     3781 atctgcccat ggaacggcga gatcctggca atggttggct cagccgattt caataatgct
     3841 gccatcagcg gtcaggtcaa cttcgccacg agcctgatcc agcccgggtc atcgatgaag
     3901 cccattgcgt acgcggcggc ctttgaaaag ggctggaacc cggggacggt catcatggat
     3961 atcccgacca cttgggatgt gccgggacag gaaccctacg agcccaacaa ctacaccaag
     4021 cagttttacg gagcggtcca cgcgcgcgag gcactggcca attcgttcaa catcacggcg
     4081 gtcaaggcca ccgattttgc cggtgtcgag gatgtgatgg atatggcacg acgaatggga
     4141 atcgtcgata gcctcgagaa cgatgccggc ttctatggac tctcactcgg ccttggtgcc
     4201 ggcgagctgc agttgcttga gcacaccaac gcgtatgcca cgctggccaa caacggccgg
     4261 tacgttcccg cgaacccgat ccagcggata caggacagcc agggaaacct gctcttcgaa
     4321 gtaacccccg aatcgatcga gaaggaagcc gtccaggcga tgaacccggg caatgcgtat
     4381 caggtcacct ccatcctcac cgacaacgat gcccgggccc gggtattcac gcgagataac
     4441 cgcttcggtg atacgcagtc ggcgttaggg cgacctaccg ccgcgaaatc gggaacgacg
     4501 gagaactggc gcgacctctg gacgatgggc tacacatccg ccgccgccgt cggtgtgtgg
     4561 gtgggccgct ccggtgatgc cggaacggcc cagctgcctg agatcgacgg tatccaggca
     4621 gccggaccga tctggcaaga cgtgatgatg ctcatacacg agacacccga gttcgccgcc
     4681 ctcctcaatg gaccggacgg ccagccggtc cccggggatt tcccggtacc ggacgaggcc
     4741 tacgaaaccg acctctgtgc ggttacggga catcgtcctg gaagcggtga cacgatcgag
     4801 gattgggtgg ttcgagatca ggagccgacc ctggcgtgta cggaagtaaa tgaatacgag
     4861 gccgagcagt tggcggacgc gctcgaggcg ctgcgcgaag gcgccaactg ggcgaatggc
     4921 gcggcggaga gcatcaaccg ctatgcccgg atggtggggc gaagcgcggt tcccatcgag
     4981 ggtgacgacg aagagagcga ggaatctgac gactccggag agacgggtgg caccgacgat
     5041 gaagagcagc cgcctatcga gccgttggac tagcgagggg ctcctctccg gtggcaccgg
     5101 ttcttgcgag gccgcagccg cgaagagatt ttgatccccc atgaaacaca gcgatgcccg
     5161 gcgagcatcg ctgtgtttca tgggtggaag cgactactcg ctggtctctt ccttctggga
     5221 agctgtcgcg gccgcctttg ggcgtccacg tcggcgcgga gccccttccc ccttcaccgc
     5281 tgactcgatg gcggcgcggc gacgacgacg cgtcagtcgc gtgcgctcgc tgaccgagcc
     5341 ttcgggtgga acgatcggcg cgtcgacgat cggtgtggaa agcacgatat tgctggagca
     5401 cttggcgcct acatcctgca gctcatcgat cagcgattcg agatcctcgg tcgaggcaac
     5461 atgcgccttc acgataaagg cgtgctcacc tgtcacccga tggcactcct ccaccgcagg
     5521 ctcggatcga aggcggttga ggaacgcgtc gtaatgccgg cggtccaggt ctacggagat
     5581 caacgccgtc agggaccgac caatcgcgtg cggatccacg atcgcgcggt agcccgcaag
     5641 gactccgcgc tcctcaagac ggcgcacccg ctcgatcatt gcaggcgaag agaggccaac
     5701 gcgttcggcc agcgccttca tcgtcaatcg accattactc tgaagttcgg acaggatacg
     5761 ctcgtccaat gtatccaggt ttgacatagc tattcctcca gtcattacgg gactagacaa
     5821 caaaatgatg cccactgggc gagtactttc gaaaacaaag gcacgttacg gaagcccgcc
     5881 cattttcaga ttgcttcact cgccaacatg aggattcttt ttgtgggcaa cgatgcctct
     5941 gcgtgcatta taagcgaaga ccgcatatag aaaagccaga accggacttc ggcgtcatta
     6001 caacgattct atggtatgcc attggcagaa ataaaccgaa ctaccctgct ctaacgggta
     6061 gtaaaggcac gctgcatcgc cgtctcatgc gcccgacgtt ccaggtcatc atcgatgtct
     6121 ggcaggtccg ctgagccgta gcggtggcga agtcccatct cgatcagcca atctgtcagc
     6181 gcagggttct ttggtaacag tgcgccatga agatagcaac cgattgcatt cctgtagatc
     6241 gcgccttcgg tgccgtcctg accgttattc ccacgtccga cctggatctt ccccaagggc
     6301 tcaacgccct cgcccaacca ggtcaaacca ctgtggttct cgaacccgac cagcgtaccc
     6361 caacggctgt tgacaacaac gttgccaatg aagcgctccg gcccagccgt ggacgccgcg
     6421 tcgaatagcc cgactcctcg caacggctcc ccattgtgag gccggtattc atggccaaga
     6481 agctgatatc caccgcaaac ggccaacatc gcggcgccgt tctcgatcgc cgtgcgtagc
     6541 tgctccgcct tttcaccctg catgtcgtgc gagacggcca cctgttcctg gtcctggccg
     6601 ccaccccaga aaaagatgtc gacatcgtcc gggatgggtt caccaaagcc gatctcacgg
     6661 atctcgacgt cgatgtccct ccagcgcgcg cgctgggcaa tcgccaggac attaccccga
     6721 tcgccataga tattcatctt ggtgccgtag agccacccga caacgaggca ctttggagat
     6781 tcggtcgtca tggaggtttc catcaggatt atcgtttcag tttcggcgca ggaaggcaaa
     6841 caccctactg ctcccagaat gcatcgacgg caccgcggtc tgcgagggtc ttgtgcagcg
     6901 ccagcattgc ggtataggac ggcagcatgt acgcggcgtg tccttcaggt accgcttcaa
     6961 caaaggcgtc gagccctgca ggaagctcgt tggggaggga gtgaatgcga tccagcgcaa
     7021 cacccgcata cttgagccgg ttcgccatgt cagggcctcg cagaccggtc gtgtagagat
     7081 cccctttgcc gcgcgccagc aactcgaaat cgacatccca gagccaggag acatcgcggc
     7141 catccgcgtc aagatcattg attgcgatca tcgttggcaa ggcgagcccg cccgtctccg
     7201 ccgtcagcat ccgcaacact tcgttgaagc cggtgggatt cttgacgaga gccagcgtga
     7261 ggtcgcgccc cttgtactgg acccgctcga tgcgtccgaa cgccgagcga aaggcgtcaa
     7321 aagcttggcc gataagctcc cagggaatgc caacctctct cgcaactgcg gcggcggcaa
     7381 gggcattgta ggcgttgtag agaccgggca cgccaaccgt gagatgatgc gtgtggcccc
     7441 cgccctcaga aatattgagc gcgagagatt ggactccctg gagctcgata ttgctccccg
     7501 tataggtgag atcgggccgc ttccgcccac agtttgggca ataccagtcc cccaaatgcg
     7561 aaacgtaaag ggcgtggtag gcgaggtcgg cgccacagac acggcaaaca gccgcatcgg
     7621 ccgcgtgggg caacacatcc aatcgatgtg tctgctcgtc cagaccgaaa cagatgcgtt
     7681 tggcggttat ggaatccgat atcacgacca gtgccgggtc atccccgttc acgatcactg
     7741 tggtccctgc gtccagatcc ttcaggacac ctcgccacgt tttcccgatc gtgtccagct
     7801 ccccatagcg atccaactgg tcgcgaaaga ggttgttcag caggacgatc cggggtcgca
     7861 cgcgacgaac gagctcctgc agcgcagctt cgtcagcctc gatcactgcg atctcgccgc
     7921 cagggcaacc cgtcagcgat gcccgctgaa caaacgcggc gatagcccca cggacgaggt
     7981 ttgagcccga gcgattgtga accacccgcc acgggacaag ctcgaagaca tcggcaatca
     8041 tccgggtggt ggtcgtcttg ccgttggttc ccgcaacgat caccgccccc tgctccaggc
     8101 cggcggcgag cttggtcagc atggcagaat ccaggcgatc cgcgaccaaa cctggcgctg
     8161 acgtgcctcc accccgcccc agtcgcctga tgacagcccc ggctaccttc gccgttgcaa
     8221 gtgacgccac caggcgcagg tctcgtccct gtgcagttcg aaattcctta tccattggga
     8281 acagctatcc agactgtcat caggttttcg aaatcggctg caggctgcac catgatctcg
     8341 agctggtcgc tctgggcatt gagagcgggt tcgccgacaa caacgccgat cgggatattg
     8401 gatggtacct gtgccgtctc ggacgccgcg acatccgaag tgacgaccca ctccagctcg
     8461 ctaggaacga catccttgtc gacatgccgc atgatgagca gacccccact gccgcgggtg
     8521 ccatagacga cgccgtcggc gcgtgcatcg tacaacatgg ccccgacagc cagagccgga
     8581 tctgtcacga gaagtacacg agcctgatgc tcctgcactt cggtgatctg gcccacgaaa
     8641 atgtcgggac tgaccgcggc cataccttga cgcaggccat ccgccgatcc cttgttgatc
     8701 gtgatgaatt gttgtgagcc agtgggatcc tgcccggtaa ccctcgcagg gacataggtg
     8761 atatcggggc gtaactgctc gactcgtacc tgctcccgga gcgccttgag ttcctccacc
     8821 tcagccttca gtcgtgcatt ttccgcgacc acgcgattac ggtcggcaac caccgttgcg
     8881 tacgctacct ccagatcgga accaccaacc ctcggctgaa cgacgccctc gaaggtactg
     8941 gcgatcggtg ccaatacttc gcccagtcca tcacgaatcg ggtcgagcag ggaacggcga
     9001 tcaaggacca ggaaaatggc tgatatcacg atgaagatga gcaccagcga cacaacgcgc
     9061 cgcattggca cgttggtcat gattcctgtc ctctatctgc cacaagggcg cactgatgcg
     9121 tcacgcgaat ctccctggat ttctcctgca caggcaacta gccgcgccgg agctcctgtc
     9181 caccagccag cgcccgctga tacagatgga gcgattcaac gacccgtccg gttccgcgag
     9241 cgacgcacgt cagcgggtcg tctgcacggt gcactggcat gcggagctcg ctctgcagac
     9301 gacggtccag gcccatcaac aaggcgccgc ctccggcgag agtaatgccg tgctccatga
     9361 tatcggcgat caattccgga ggggtctcct cgatggaggt cttcaccagc tcgatgatgg
     9421 tgttcaccgg ctgcgagatg gcatcgcgca gctcgacaga gctcacctcc accgatttcg
     9481 gcaggcctgt aaggaggtca cggccacgca gcatgaccct gacctcctcc tcaagggggt
     9541 aggccgagcc ggcggcgatc ttggcgttct ccgccgtccg ctcaccgata accagattgt
     9601 ggtcccgccg ggcgtactgg atgagggcat catcaatctc atcgcccgcc acacggatcg
     9661 agtgattgac cacaatgcca cccagtgaga taacagcgat ttcggtcgtg ccgccgccaa
     9721 tatcaacgat catgctgccg atcggctcat tgatgggcag ccccgcaccg atcgcggccg
     9781 ccatgggctc ctcaatcgtg taggcctcgc gggcgccggc ggagagagct gcgtccttcg
     9841 ccgcccgctt ctcaacctcg gtcacaccgc tgggaatacc gatcaccact cgtgggtggg
     9901 gtgccatcag gcgctgcgta tgcaccttgc ggatgaagtg atgcagcatc atctcgaccg
     9961 tgtcgaaatc cgcgatcacg ccatcctcga gcggacgcac agcaatgatg gactccggtg
    10021 ttttgccgat catggctttt gcttccatgc cgacccacaa tgcccgcttc gttcttgtat
    10081 caatggcgac tacggaaggt tccgagataa caatgccctt gcctcgtaca tacacaaggg
    10141 tgttggctgt cccgagatca atgccgagat cccggctaaa gaagccgaac agggtggaaa
    10201 gcggacggaa caaagaagtg ggacctccgg catagcatcc atcatgactg gatcatcagt
    10261 ggagcatcgg aaagagatga tcgggacaga ttcagtattg aatatcggag ctgcgaaggc
    10321 gtggtcggaa tgatcatgcg ggggcaggac cggcgcctga gttcccctcc gggtggcttg
    10381 ccgaaccgac gagcaaccct ccatcggtgg cgcctggcac gctccggagt cgggcggagt
    10441 atagcatagg caccgctcct cacccgccgg acactcccgg gggaagccgt ttcaccgcac
    10501 ggctgagggt aaatgcggta cgtcacctag gcgtacctgt gagccagctt ctccaatgtc
    10561 cgctcgatcc cggaacggtt tttttccggc acgccgagca tctcgtatac cctcgccacc
    10621 ttgcagtcgc gatagtcgaa cgtctcggtg acccttgtct ccgagggacc caagggctcg
    10681 aattcgtatc tccaccgatg tccggcggga tgccgccact cgacgacctt cccgggttct
    10741 gcccgcgtca ccgtgctggt gatcatgtac gggactccat atatcctcat gtgcactgtg
    10801 aagcgatcac cttcgttgag tcgctctggc ccctcggcct ggtcacgcac cgtgccgctg
    10861 ccatccagct catgatggcg gtgcgggttg gccacgagtg cgaagagctc atctacaggt
    10921 gcattgacca caatgctgcg agcaacctgt cgatcgcctg tgtcgacggt cattggtgag
    10981 gaagtgtgca taatcgacag cctttcctga acgggccgga tacatattcc ccgcatatcc
    11041 ccgttgcgac catgttccca tacaatgccg cacatgcgca gttcacgtga aggtaaggcc
    11101 cgcatggtta ggaaacaggc acaggaagac ggctgggcga cgatccgcga cgttttgtcg
    11161 tcccgacccg atggcaccga ggtcgggcta tcggtctatc tctttgggag cggccagacc
    11221 ctctccatcg atggagaaag agatttcgcc tccgccagta cgatcaagat cctcatcctc
    11281 gccgcgctgg cccgcggctt tgatgagggc cggttctcac cggaggacaa gatcgccgtg
    11341 agagaagacc tgaagttgga aggcagcggc gtcctcaact ggctggagac cggcctgagg
    11401 ctctcccttc acgaccacgc gtggctgatg acggcgatct cggacaatac cgcttcgaat
    11461 atctgcatag agacagtcgg actcgatgag atcaacgcac ttggcaacga gattggcgtc
    11521 gggaagacgc atctcggtcg catgttcatg ggagccaacg ctcctcctgg ccccccgaag
    11581 aaccgggcaa ccgccgacgg gctcgttgcg atcctgaagg cgatcgagaa cgacaccgct
    11641 gcctcgccag aacggtgcgc ctggatgcgg tcatgcctgg atgaccagca gcatgtggac
    11701 cgcctgcctc gccatctacc cgaggggatc tcgtatcgag gcaaaaccgg cacgatccaa
    11761 gggattgcac acgattgcgg tgccctcacc gggccgggtg gcaggatcgc cctcgcggtg
    11821 ctcaccgagg gcttcaggaa tccctacgac gcggaccgac tgatcggccg catcggcgcg
    11881 gccgctgcct cacttgtcgc cggaaacgat tgagtgccgg gtcaccccta ttgccaactc
    11941 ctgccctgat gcacctctcc acgatgccgc cacggtgccg tggcggcggc agcggtgcta
    12001 acgggcacgc cacaggcagc aagagcattc aggaaccgtg tccgctgctc cttccctacc
    12061 aggccgggag cagggacgtc gaagtcgaat agcaggtcgt actgctggtc gccctgatga
    12121 aatgaaacgc ggattcgatc ggatacgcca tcgcgcgcgc cggcgatatc agaga
//
LOCUS       DASYWM010000249         5599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_330396, whole genome shotgun sequence.
ACCESSION   DASYWM010000249 DASYWM010000000
VERSION     DASYWM010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330396"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VGR29_10235"
     CDS             complement(<1..200)
                     /locus_tag="VGR29_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HEV2074006.1"
                     /translation="MSEPVDLQELDRMRPPAEAREIIAAYLRRLGMEHVPLMEAIGRV
                     LVQDITAPEDHPPFPASTMDGYA"
     gene            326..1705
                     /locus_tag="VGR29_10240"
     CDS             326..1705
                     /locus_tag="VGR29_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEV2074007.1"
                     /translation="MRNTVTPQRLRWILILAPLASVVAVEAVRHFLLPEFFASWSGFL
                     TLLVMWLLGSSLFTRFIVGQVDGRHRMVQHQNDELLALHHASLATESELDLERVPERV
                     VDEARQLLGVKYGGLTFFRDDGEIGAFITSGITPDVADAIGLPPKGHGVIGVETKTGR
                     TLRLDDISTHPESVGFPPHHPPMKPLLAVPIRSKNGVFGHLYLADEAGFQFGIRDEET
                     HVRFAALSAVAIENAQLHRQVRTLAITEERERIAREMHDSLAQVLGYVNTKAQAAKVL
                     IASNQTERASAQLDQMATAARSAYADVREGILSLRTSLQPDRSLTDTLREYLAIWQEQ
                     SGVSVTLDDSRIPVGALSDLAEVHLLRIVQEALANVRKHAGATEIWIVIEGTGPDVVT
                     TFADNGLGFVIQEREYRGVPQFGLSTMRERAESLGGSFTVTSIPGEGTVIQVRLPAER
                     ARSPEGSAS"
     gene            1711..2361
                     /locus_tag="VGR29_10245"
     CDS             1711..2361
                     /locus_tag="VGR29_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2074008.1"
                     /translation="MIADDHALFRDGLRSLLEAQGIEVLAEARNGNEAVELARLHRPE
                     IVLMDLNMPQVDGLAATRLISVEMPDVRVVVLTASDDDADLFEAVKSGAQGYLQKSLE
                     AEELFAFLEGVLKDEPALTPVLARKVLGEFIRAPQEPAPQPFGSTLTEREQDVLELLV
                     SGVTSNRDLADKLFISENTVKYHLRNILDKLHLNNRAQVVAFAVRHGLVDPGKSGT"
     gene            2586..3356
                     /locus_tag="VGR29_10250"
     CDS             2586..3356
                     /locus_tag="VGR29_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2074009.1"
                     /translation="MLEMIVMVIQAPEISATRILLSVASPQLLPAATAFVTTTVGEGA
                     DIQIVEPDELAELSGAAKHERPAMIVIVGYPGDDAVCEAVLDPAGIALRTLVPVMFFR
                     GADGDPVSWSTTKRILVPLDGSSQSAQVLSVAARIARTTGVPVHLVMVIDPSRVLPPA
                     YAYDPDPWEVIAGLRESAHWALRQAEEPLMREGLPVESSLLYGPVSTVLQSTLTPGDM
                     VIMTIRGTGMALCNANSVARRVLAAATGPVIIHPERSW"
     gene            3471..3941
                     /locus_tag="VGR29_10255"
     CDS             3471..3941
                     /locus_tag="VGR29_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074010.1"
                     /translation="MDRFTIIMVKASLVWLVMGVVIGGLMLVDRGIPGNWRQWLLPTH
                     GHMLFVGWLVQFALGIAYWLLPRKRNVELPVGYRENTALAGAVMLNLGLALRVLGEPL
                     ERTGHASNVSLGLLTASSMLQVVAIVIFVAQLWPRIYGKGRMGKASPATPHKPS"
     gene            4215..>5599
                     /locus_tag="VGR29_10260"
     CDS             4215..>5599
                     /locus_tag="VGR29_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV2074011.1"
                     /translation="MEHDKNPRQRVAPLEMSPDHFRVLGHQLVDRIAEFLAGLPNGSV
                     TPGESLAVVRQALGGEGLPQHGSDPDRLLEEAASLLFNHSMFNGHPRFFGYITSSPAP
                     VGALGDMLAAAVNPNVGAWSLGPMATEIEVQTVRWIAELVGYPTTCGGILVSGGNMAN
                     FVGLLASRRAKAGWDVRSGGMAANPGGALRVYASVETHTWLQKGADLFGLGTDALRWI
                     PTDARQRMDVAALRAAIVSDEAAGECPFLVVGTAGSVSTGAVDPLPELAAICREHGLW
                     FHVDGAYGGFAAALPDAPPDLLGLCEADSVTVDPHKWLYAPLEVGCTLVRDPQALLSA
                     FSYHPPYYRFDGSDDTMVNFHEYGPQNSRGFRALKVWLGLRQAGREGYVRMITDDIAL
                     ARTLHRHVSECPELEPWTQDLSITTFRYVPLGLEPGDEHIEAYLNRLNVEILTRLQEG
                     GEAFLSNAVVSG"
BASE COUNT         1067 a         1595 c         1780 g         1157 t
ORIGIN      
        1 gcatagccgt ccatcgtcga tgccgggaag ggcgggtgat cctccggcgc ggtgatgtcc
       61 tggaccagaa cgcgaccaat ggcctccatg agggggacgt gctccatccc gagccggcga
      121 agatatgccg caataatctc gcgcgcttct gctggcggtc gcatacgatc gagttcttgc
      181 agatcaacgg gttcactcat tggtgcctcg tggtgtaacg atgatgcacg ccgctgttgc
      241 catgtgcacg aagtctctgg gcggcatcct accatgaccc tcttacaatc cagtgaggat
      301 ggtagagccc agggccggag ggctgatgcg aaatacggtc acaccccagc ggttgagatg
      361 gatcttgatt ttggctccac tggcatcggt cgtggctgtg gaggccgttc gtcatttcct
      421 gctgccagag ttctttgcat cgtggtccgg attcctcacg ctcctggtga tgtggctcct
      481 cgggagctcc ctctttacgc gcttcatcgt cggccaggtc gacgggaggc atcgaatggt
      541 gcagcaccag aacgacgagt tgcttgccct gcatcacgcc agtctcgcca ccgaatcgga
      601 gcttgatctg gagcgggtcc cggagcgcgt ggtggatgag gcacgacagc tgctgggcgt
      661 gaaatacggg ggattgacct tctttcgcga tgatggggag atcggcgctt tcatcacctc
      721 cggtatcact cctgacgtcg ccgatgcgat cggtctacca ccaaagggac acggggtgat
      781 cggggtggag acgaaaacag ggaggacgtt gcgactcgat gatatttcga cccatcctga
      841 atcggtcggt tttccgccac accatccgcc aatgaagccg ctgctggcgg tcccgattcg
      901 atccaagaac ggtgtcttcg gtcatctgta tctggcagat gaagctggct tccagttcgg
      961 catacgggat gaggagacgc acgtccgctt cgccgcactg agcgcggtgg cgatcgagaa
     1021 cgcccaactg caccgccagg tgcggacatt agccatcacc gaagagcgcg agcggatagc
     1081 ccgcgagatg cacgacagcc tggctcaggt cctcggttat gtgaatacca aggcgcaggc
     1141 ggcgaaggtg ctgattgcat ccaaccagac agagagagcc tcagcgcagc tggaccagat
     1201 ggccaccgcc gccaggtcgg catatgccga tgtgcgcgaa ggaatcctca gcctgcgcac
     1261 gtcgcttcag ccagatcgct cgctgaccga cacgctgcgc gaatatctgg ctatctggca
     1321 ggagcagagt ggcgtcagcg tgacgctgga cgatagccgg atacccgtcg gtgcgctttc
     1381 tgacctggcc gaggtgcatc tgttgcgcat cgtgcaggag gcactggcaa atgtgcgcaa
     1441 acacgccggg gcgactgaga tttggattgt tatagaggga acaggcccgg atgtcgtgac
     1501 cacgttcgct gacaatgggc tcgggttcgt gatacaggaa cgcgaatatc ggggtgtgcc
     1561 gcagttcggc ctgagcacca tgcgcgagcg agccgaatcg ctcggcgggt cgttcacggt
     1621 gacgtcaata ccgggtgagg ggaccgttat acaggtgcgc ttgccagcgg agcgagccag
     1681 gtcgccggaa gggagtgcct cttgagagtc gtgatagccg atgaccatgc cctttttcgg
     1741 gatggcctga gaagcctcct ggaggcgcag ggcatcgagg tgctggccga ggcaaggaac
     1801 gggaatgagg ccgtcgagtt ggcccggctc catcggccag aaatcgtgct gatggacctc
     1861 aacatgcccc aggtggatgg ccttgccgca accaggttaa tttctgtgga aatgccagat
     1921 gtgcgcgtgg ttgtgctgac ggcgtccgat gacgacgctg atcttttcga ggcggttaaa
     1981 tccggcgctc aggggtatct gcagaagagc ctggaggcgg aggaactctt cgcgttcctc
     2041 gaaggtgtcc tcaaggatga gccggcgctt acgccggtgc ttgcaaggaa ggtactcggc
     2101 gagtttattc gagcacccca agagcctgca ccgcagccgt tcggttccac gttgacggag
     2161 cgggagcagg atgtgctgga gttgctcgtc agcggtgtga catcaaaccg ggacctggca
     2221 gacaagctct ttatcagtga gaacacggtg aagtatcacc tgcgaaacat cctcgacaag
     2281 ctacatctca acaatcgggc ccaggtcgtg gcgtttgcgg tccgccatgg cctcgtcgat
     2341 ccaggcaaga gcggcacgta agccaggaca tttttgaggt cgagggaaac gacgaagcgt
     2401 gggtcagttc ctccaaatgc aggtggatgc aaccaaaccc gacccgtcgg cgttcccctg
     2461 ccataccctc tctcgcagag agtgatcccg gtgcgttccg tatagccacg gtcttcaacc
     2521 tcgaggagca ctcctcatcg gcgggcgccc gtcggtgctt cccgatgtcg cgatgtggat
     2581 gtggcatgtt ggagatgatc gtcatggtaa tccaggcccc ggagatttca gcgacccgta
     2641 tcctgctttc ggtcgcgtcc ccacaactcc tgcctgcagc gacggctttc gtcaccacca
     2701 ctgtgggcga aggagccgat atccagatcg tggagccgga cgagcttgcc gaactgtccg
     2761 gtgccgcgaa gcacgagcgc ccggccatga tcgtcattgt gggctatcca ggtgatgatg
     2821 ccgtgtgcga ggcagtgctc gatcccgcgg gaatcgcact taggacactg gttcccgtca
     2881 tgttcttccg aggggcggat ggtgatccag taagttggtc cacaacgaag cggattctgg
     2941 tgccgctcga cggctcctcc cagtcagcgc aggtgttgtc cgttgcggca agaatcgcca
     3001 ggacgactgg ggtgccggtt cacctcgtca tggtgatcga tccgtcgagg gtcctgcccc
     3061 cggcatatgc ctacgatcca gatccctggg aagttattgc cggactacgc gaaagcgcac
     3121 actgggccct ccgtcaggca gaagaaccct tgatgcgaga agggctccct gtcgagtcct
     3181 cattgcttta tggaccggtg agcaccgtcc tgcaatcgac tttgacgcct ggcgatatgg
     3241 taatcatgac aattcgggga actggcatgg ccctatgcaa tgcgaatagc gtcgccagac
     3301 gcgtgctggc agccgccaca ggtccggtca ttattcaccc agagcgatca tggtgaaatc
     3361 gtcgtggatg gatatgaggc gtgttcctgg gccgagccgt tgtctcgcta gcccacaatg
     3421 tatggtcatt aaagttcgac aggcaaatcg cgcacgggtc tggaccgagg gtggatcgtt
     3481 tcacaatcat catggtaaag gcttcattgg tgtggctggt gatgggtgtt gtcatcggcg
     3541 ggttgatgct cgtcgaccgc ggcatcccgg gtaactggcg ccagtggctg ctgcccacac
     3601 acgggcacat gctctttgtg ggttggctcg tgcaatttgc actcggtatc gcctattggc
     3661 tgctgccacg gaagcggaac gtcgagttac cagttggcta ccgggagaat acggcccttg
     3721 ccggagcagt gatgctgaat cttggtcttg cgctacgtgt gctgggagag ccgctggagc
     3781 gtaccgggca cgcgagcaat gtgtcactcg gacttctcac ggcatcctcg atgctgcagg
     3841 tcgtcgcgat cgtcatcttc gttgcgcagt tgtggccacg catctacggg aaggggagga
     3901 tgggcaaagc gtcccctgcc acaccgcata agccttcctg agagttccgg aacattgaag
     3961 aggcagtacc ttcgatacct gccagtccgg tacggtggag tccgatggga caatcgtagt
     4021 ggtccagccg gctgtctgtt cggagctgac actgcgtttc ctttagcggc gtctgcgcgt
     4081 ccccagatgc cagtatcaat cctcacaccc gtatctgttg aacacagacg gtgctacact
     4141 ggccatgact ctctaacaat ccataagcct ggtctcagcg agacacttcc ggaagcgagc
     4201 agccaatggc aagcatggag catgacaaaa accctcgtca gcgcgtagca ccgcttgaga
     4261 tgagcccaga tcactttcgc gtacttggcc atcaactggt tgatcggatc gccgagttcc
     4321 ttgccgggct gccaaatggg tcggtgactc caggggagtc actcgccgtc gtccgtcagg
     4381 ccctcggagg ggagggcctg ccccaacacg gttctgatcc tgaccggctg ctcgaggaag
     4441 ccgcgtccct tttgttcaac cattccatgt tcaatggcca tccccggttc tttggataca
     4501 tcacatcatc acctgcaccg gtgggggcgc tgggtgacat gcttgcagcg gcggtgaacc
     4561 cgaacgttgg cgcgtggtcg cttgggccca tggcaacaga gatcgaggtt cagactgtac
     4621 gatggatcgc cgaactggtg ggatacccca caacctgtgg aggaatcctg gtcagcggcg
     4681 gcaatatggc gaacttcgtc ggactgctgg catcacgccg ggcgaaagct ggctgggacg
     4741 tgcggagcgg cggaatggcg gcaaatcccg gaggcgcact gcgggtatat gcctccgtag
     4801 aaactcatac atggttgcaa aagggggctg acctctttgg ccttggcacg gatgcactgc
     4861 gatggatccc gacggatgca aggcagcgca tggatgttgc cgcgttgcgc gcggcgatcg
     4921 tctcggatga ggcggcaggc gagtgcccgt ttctggtggt ggggacggcc ggatcggtga
     4981 gcaccggcgc ggtcgatccc ctgcccgaac tcgctgcgat ctgtcgtgag catggattgt
     5041 ggtttcacgt cgatggcgcc tatggcggat tcgccgctgc cttgccggac gcacctccag
     5101 atctgcttgg gctgtgcgag gccgactcag ttaccgtcga cccgcataag tggctatacg
     5161 ccccacttga ggttggttgc acgcttgtgc gggatcctca ggccttgctg agcgccttca
     5221 gctaccatcc gccgtactat cgctttgacg gctccgacga caccatggtg aattttcatg
     5281 agtatgggcc ccagaactcg cgaggcttcc gcgcgctcaa ggtctggctt ggcctgcggc
     5341 aggccggtcg tgagggatac gtccggatga tcacggacga catcgcgctc gcccgaacgc
     5401 tgcaccggca cgttagtgag tgcccggagc tcgaaccctg gacgcaagac ctcagcatca
     5461 cgacctttcg ttacgttcct ctgggcctcg agccaggtga cgagcatatt gaggcttacc
     5521 tgaaccggct caatgttgaa atcctgaccc ggttgcagga ggggggagaa gccttcctct
     5581 ccaacgccgt ggtgagtgg
//
LOCUS       DASYWM010000250         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_367728, whole genome shotgun sequence.
ACCESSION   DASYWM010000250 DASYWM010000000
VERSION     DASYWM010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367728"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2264
                     /locus_tag="VGR29_10265"
     CDS             <1..2264
                     /locus_tag="VGR29_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV2074012.1"
                     /translation="KDDFMTDTKSKKAAPETDETGEQVVAAPQIEEPTQSLEEAVDAA
                     AEESDATDIFFDTPVSIPGAPEASLDGAWLAFLKPNDDGALELWIAPTDGGEAHHLHL
                     PFIPLEDVNPETGRIVRGPQWSSDGSRIALTGLHPDGDRTAVWIVPTGVSATAGEEPA
                     TVVNDATSSTSPESEGGDPDGGQEIVTPAAPEGVGEGMPLPLPKMDNDDAMRELEELE
                     EVESAPAPDVTAPEEEPTSPAPERARMLVDHSGSDRSPRWSTDGEIIAMTSTIDGRDV
                     ISLAPVQGGDSSAIELLTWSRSNDREPVWSRDGNHLAFLRQRPDGPEHADIFCFFLET
                     GELKNLTSDKASAVRHSLEWVPGRNLIAYVTHENDWLSISVINADNKAGWTVTRESGD
                     KTEPRFAQNESRMVYVRTEGFTTVLCERSLHASSAVALDPGEGVVRYPRWLNAKRVAY
                     GFSAPQRPFGFLVQDNLADADRTPVSVPGMPSLAGHAMRQPQPFEFEVGPEEMFSGML
                     YKTHGVAGRVPTIVYMPDGPLATRRGEFQIEEQALASTALTVLAPVIHGASGFGLAVE
                     NDLRDFAGTELETFDLAEVGRALAREEGIARDQIALVGSGYGGALALVTAGARPGVYA
                     AVVAIDPVTDWSIELANCEIPWRNWVTDRFGMPLTDADHYTLRTPATFSAVIDAPLVL
                     VRTASAPEYRKAQMDLFTQDLDEQGVAYEVYEAGEETLPGTLREISKMLTRRFLGGAE
                     MADVVADLKSDNL"
     gene            2496..>3430
                     /locus_tag="VGR29_10270"
     CDS             2496..>3430
                     /locus_tag="VGR29_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEV2074013.1"
                     /translation="MSAHPRQQARPSRKPSQRQQGFTRREFVSASVGAAVAGTAGLVA
                     WKSGVGPGPGGRTIAAVTQTPTPLSNGGVGTPSSASGIVIDPTATPYQTATPEPRSAA
                     SQMVVETSIGELRTAYDKGWFTVSEYVEAALGRINAMDAQGPSLNALIELNPDAMDIA
                     AQLDREAQQGRVRGPLHGVPVLVKDIFATNDQMRTTSGSLSLQENTVVRDAFLIEMLR
                     NAGAIILGKTNLTEFSNFRGGTPNGWSSRGGQTVNPYVLTHSAWGSSSGSAVVTAASY
                     VPFAVGSETDGSIICPASACGVVGLKPTVGLVSRNG"
BASE COUNT          646 a         1071 c         1108 g          605 t
ORIGIN      
        1 tgaaagacga tttcatgacc gataccaaga gcaaaaaggc cgcgccggag acggacgaga
       61 ccggcgagca ggttgtcgcg gcccctcaaa tcgaggagcc gacgcagagc cttgaagaag
      121 cggttgatgc tgctgctgaa gaatcggatg ccactgatat cttcttcgac accccagtat
      181 ccatccccgg cgcaccggag gcttcgctcg atggcgcatg gctggcgttc ctgaaaccaa
      241 acgatgacgg agcgcttgag ctctggatcg cccccaccga cggtggcgag gcacaccacc
      301 tgcaccttcc ctttattccc ctggaggatg tgaatcccga aacagggcgc atcgttcgcg
      361 gtccgcaatg gtcgtcggac gggagcagga ttgcgctgac tggtctgcat ccggacggtg
      421 accgcactgc tgtctggatt gtcccaaccg gtgtcagcgc cactgccggc gaagagcctg
      481 caacggtcgt gaacgatgcg acgtcgtcca ccagcccgga atcagagggt ggcgaccccg
      541 acggcgggca ggagatcgtc actcctgccg ctccggaagg agtcggcgag ggcatgccgc
      601 tgcccctgcc gaagatggac aacgacgatg cgatgcgcga gctggaggag ctcgaggagg
      661 ttgaatcggc accggctccg gatgtaaccg caccggagga agagccgacc tcccctgcac
      721 ctgagcgagc ccggatgctg gtcgatcatt ccgggtcgga tcgatccccg cgctggtcga
      781 cggatggcga gatcatcgcc atgacgagca cgatcgacgg tcgcgatgtg atctccctgg
      841 cgccagtcca gggtggagat tcctcggcta tcgaattact cacctggagc cgcagcaacg
      901 atcgtgagcc ggtttggtcc cgagatggga accaccttgc gtttctccgc cagcgccccg
      961 atggccccga gcacgccgat atcttctgct tcttcctgga gacgggggaa ttgaagaacc
     1021 tcacctctga caaggcgagc gccgtgcggc actcactcga gtgggtgccg gggcgtaacc
     1081 tgattgccta cgtcacgcat gagaatgact ggctctccat ctccgtgatc aacgcggaca
     1141 acaaggctgg ttggacggtc actcgtgaat cgggagacaa gacggagccg cggttcgcac
     1201 agaacgagtc ccgcatggtc tacgtccgca ccgagggctt caccaccgtc ctctgcgaac
     1261 gaagcctgca cgcctcgagt gccgttgcgc tggatcctgg cgaaggtgtt gtgcgctacc
     1321 cacgctggct gaacgcgaag cgcgtggcgt acggtttttc cgcgccgcaa cgaccgttcg
     1381 gcttcctcgt gcaggacaac ctggcggacg cggaccgcac cccggtatcg gtgccgggga
     1441 tgccttccct ggccgggcac gcgatgcggc agccgcagcc attcgagttc gaggttggcc
     1501 cggaggagat gttctccggc atgctctaca agacccacgg ggtggcgggc agggttccca
     1561 cgatcgtcta tatgccggac gggccgttgg caacccggcg tggcgagttc cagatagaag
     1621 agcaggccct cgccagcacg gctctgacgg tgcttgcccc agtcatccat ggcgccagtg
     1681 gcttcggcct cgcggtggag aatgatctcc gggatttcgc gggaactgag ctggagacgt
     1741 ttgacctggc cgaagtgggc cgggcgctcg cgagggaaga aggcatcgcg cgagaccaaa
     1801 ttgccctggt gggatccgga tacggcggcg cgctggcgct ggtgactgcc ggcgcacggc
     1861 caggggtcta cgccgcagtc gtcgcaatcg acccggtgac cgactggtcg atcgaattgg
     1921 cgaactgcga gattccctgg cggaactggg tcaccgatcg attcgggatg cccctgacgg
     1981 atgccgatca ctacaccctg cgcacgccag ctaccttctc ggccgtcatc gacgcgcctc
     2041 tggtgctggt tcggaccgcg agcgcgccgg agtaccggaa ggcccagatg gacctcttta
     2101 cgcaggacct cgatgagcag ggtgtggcat acgaggttta cgaagccggc gaggagacgc
     2161 ttcctggcac cttgcgcgag atcagcaaga tgttgacgcg aaggttcctt ggtggtgccg
     2221 agatggcgga tgtggttgct gacctgaagt ccgataatct ctagaagcct cgatcgcacc
     2281 aggcactcgc atgagggcgt tcactctata accggagtgg acgccttcat gtatcattag
     2341 cgttcgtacg gggtgagatc gccccatgga cgtacccttc ctgctcagcg ttgaggctgg
     2401 atcaacggag cgtaggggcg tccacgtgga gccacagaat gaaccaacga cggccttcag
     2461 gccgcccgaa tgctcttgct ggtcatggat caactgtgag cgcccatcct cgacaacagg
     2521 cgaggccgtc caggaagccg tcccaaaggc agcagggatt cacccggcgt gagttcgtga
     2581 gtgcctcggt gggtgctgcc gttgccggca ctgccggtct cgtcgcatgg aagtccggtg
     2641 tcggcccggg acccggcggt cgaacgatcg ctgcggtaac gcaaacccct accccgctat
     2701 ccaacggcgg cgttggcact ccctcgtcgg cttccgggat cgtcatcgat ccaaccgcaa
     2761 ccccctacca aacagcgaca cccgagccaa gatcggccgc atcgcagatg gtcgtcgaga
     2821 cgagcatcgg agaactgcgc acggcctacg acaagggctg gttcaccgtt tccgagtacg
     2881 tcgaggcggc gcttggccgc atcaacgcca tggacgcaca agggccatcc ctcaacgcgt
     2941 tgatcgagct caaccccgat gcgatggata ttgcggcgca actcgaccgt gaagcgcagc
     3001 agggtcgggt gcgcgggccg ttacatggtg tgccggtgct ggtcaaggat atcttcgcaa
     3061 cgaacgacca gatgcgcacg acatccggat cactctcgct acaggagaac acagtcgttc
     3121 gcgatgcgtt cctgatcgag atgctccgga acgccggggc aatcatcctc ggcaagacca
     3181 acctcacaga gttctccaac ttccgggggg gcacccctaa tggctggagc tcgcgcggtg
     3241 ggcagacggt taatccctat gtcctgaccc actcggcctg gggctccagc tctggctccg
     3301 ccgtggtgac ggcggccagc tacgtcccct tcgccgtggg atcggagacc gacggatcga
     3361 tcatctgtcc cgcctccgcc tgcggggtcg tcggcctgaa gccgactgtt gggttggtca
     3421 gccggaatgg
//
LOCUS       DASYWM010000251         4035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_199463, whole genome shotgun sequence.
ACCESSION   DASYWM010000251 DASYWM010000000
VERSION     DASYWM010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199463"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VGR29_10275"
     CDS             complement(<1..322)
                     /locus_tag="VGR29_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074014.1"
                     /translation="MSEQLDPAQAQHNMWKWKWRRRVAVGQGQYPAQLVLGNARIVNV
                     FTEELIEGDIAIESGYIAGIGTYPNAVETIDLGGAVVAPSFIDAHIHLESSLLWVPEF
                     ARAVV"
     gene            complement(419..913)
                     /locus_tag="VGR29_10280"
     CDS             complement(419..913)
                     /locus_tag="VGR29_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074015.1"
                     /translation="MSFRINETDLDDLDPRDVIARDRLSLTPVLESLQRAGFYAYGTL
                     DDQSRWSIAVDDELGRVDVRVGDDGFEIILWVSSPGLYADEENIWKRNSRSRLARMRI
                     PRIAQGFLEPHQSAMWDEVEEGVAVSEHYQLPFNRAADVGQFVRTQLPKLEDVLSGIE
                     RQLG"
     gene            1317..2687
                     /locus_tag="VGR29_10285"
     CDS             1317..2687
                     /locus_tag="VGR29_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV2074016.1"
                     /translation="MVDSRFATLVDQMRTGAIDRRTFLVRGAALGISAGALMRAMPAM
                     AQDASPAAAGGGSDELVPENLGVAGIEHSTDTSKGTINLYSSWPMTGASEQIGGDSAE
                     SVRLAIELWGGAAGGFAINYEALDDGVAANQGSWDAAKEAENASMVVNDQDAVAYIAT
                     YNSGAAEAAIPITNEAGLAQISPANTAVQLTKESPANPEGYPDVLYPTGERNYMRVVP
                     ADDLQGSAGANFAFNTLGAQTAYILHDQQTYGRGVAESFQATFVELGGEILGFEGFDP
                     NAPEYQALMTKIASTGPQLVYLGAIVNLNASKLLQDFRGLLSPEETAFMGPDGLLNQA
                     FVDGAGDAAEGAYITFGGLPANALEGPGAVWAETMRERLGHEPDAYAAYSFEAAVVVL
                     QAIDAVGEKDRAKILETMFNTTDFRGLTNTWSFTETGDPDTATLSVNIVKDGEITFGE
                     TISPPE"
     gene            2952..3935
                     /locus_tag="VGR29_10290"
     CDS             2952..3935
                     /locus_tag="VGR29_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2074017.1"
                     /translation="MQWTLFFQFLIIGLATGALIALIALGYTLVYGIIELINFAHGEV
                     FMMGAMFAATVVSLMGVTNESTSLVIVGTLLIGLVLSMAFCALLNAGIDRIAYRRLRH
                     APRLAPLIAAIGMSFILQNIAIYWQGANPITPPNIIPSEYKTYNILKEWPILRDWFAD
                     STLRFRLMDLVVMGTTIPLLIILTWFVYRTRIGTAMRATAQDREAAALMGIDINRTIA
                     VAFLLGGSMAGAAGMIALFYNNSARFQMGFQYGLYAFTAAVLGGIGNLNGAVIGGFMI
                     GLIWAYSDGFMGLYIGGWGSQWSNSVIFSILVLVLVFRPAGLFGHSAPEKV"
BASE COUNT          808 a         1139 c         1227 g          861 t
ORIGIN      
        1 ggacgacggc acgtgcgaac tcaggtaccc agaggagcga gctttcgaga tggatgtggg
       61 catcgatgaa ggagggagcc acgaccgctc cgccgagatc aatggtctcg accgcgttgg
      121 gatatgttcc gataccggcg atatatcctg attcgatcgc gatatctccc tcgatcagct
      181 cttcggtgaa gacgttcaca atccgtgcat ttcccaggac cagctgtgcc gggtactgcc
      241 cctggccaac ggctacccga cggcgccact tccatttcca catattgtgc tgcgcctggg
      301 ccggatcaag ttgctcggac atggtgatgg atattcctca atgtggccgt tagcggcaca
      361 ggcataatgt ggcatccagt gaaaggaccg ccggtatata cagacgcaca cgcatcccct
      421 agcctaactg acgttcgatg cctgacagaa cgtcctcaag cttcggcagc tgcgtgcgaa
      481 cgaactgccc cacatccgct gccctgttga atggaagctg gtagtgctcg gagacagcca
      541 cgccttcctc cacttcgtcc cacatcgcgg attgatgcgg ctcaagaaat ccctgggcaa
      601 tgcgagggat cctcattcgc gcaaggcggc tgcgggagtt gcgtttccat atattctcct
      661 catcggcgta gaggccggga gaggacaccc aaagaatgat ctcgaaaccg tcatcaccta
      721 cgcggacatc gacgcgtccg agctcatcgt ccacggcgat gctccagcgc gattggtcgt
      781 caagtgtgcc gtacgcgtag aatccggctc gttgcagcga ttccagcacg ggggtgagcg
      841 agagacgatc ccgggcaatc acgtcacgcg gatcgaggtc gtcgaggtct gtctcattga
      901 tgcggaaaga cacgctggaa gactcctggg cactgaaacg tagcagctgg tggagatcgc
      961 ccgggttggg ggcgttctcg ggtgctggaa ttgtacgggt aatccatatc gctatgcgtc
     1021 tcggtgttgg gatcatcccg catctatact gtatttttcg ttggcaacag ttgacaatcc
     1081 gccaggattg ccggtattgt tccggccacg ttcgacaact gatgtatccg gtctcgctgt
     1141 cgaccgaaga cgctcgctgc agatcgtgat tcctgaacgg acagagctca atcaaaggaa
     1201 cgtcattttt tgatgttcgt ccacagtagt tgcggttcgt aatgaatggg gtggtcccat
     1261 tcacggtcca atccacaggt cggtaggtgc cggccagaac gcggaggtct cgggacatgg
     1321 ttgattcacg gtttgcgacg cttgtcgatc agatgcgcac cggcgccatc gatcgacgta
     1381 cattcctggt gcggggagcg gccctcggta tctcggctgg agcgctcatg cgcgccatgc
     1441 cggctatggc acaggacgca tcgccagcgg cggcaggtgg tggctctgac gagctcgttc
     1501 ctgagaatct cggcgttgca ggtatcgagc acagcacgga caccagcaag ggtacgatca
     1561 atctgtactc ctcctggcca atgacaggcg catccgagca gattggtggc gactctgccg
     1621 agtcagtcag gctggcaatc gaactttggg gtggcgctgc tggtggcttt gccattaact
     1681 acgaagcact ggatgacggt gtcgctgcga accaaggttc gtgggatgca gccaaggaag
     1741 ccgagaatgc ttccatggtg gtgaacgacc aggacgcggt cgcgtacatt gcaacctaca
     1801 actcgggcgc ggcggaagcg gcgatcccga tcacgaacga agccggcctt gcccagatct
     1861 cgcctgcgaa caccgccgtg cagctgacca aggaaagccc ggccaacccg gaaggatacc
     1921 cggacgttct ctatccgacg ggcgaacgca actacatgcg agtcgtcccg gcggacgacc
     1981 tgcagggctc cgcaggggcg aacttcgcgt tcaatacgct cggcgctcag accgcgtata
     2041 tcctgcacga tcagcagaca tatggtcgag gcgttgccga atccttccag gcgactttcg
     2101 tcgagcttgg cggggaaatc ctcggtttcg aaggctttga cccgaatgcg ccggaatacc
     2161 aggcattgat gaccaagatc gcttcgacgg gcccccagct ggtctacctg ggcgcgatcg
     2221 tgaacctgaa cgccagtaag ctgcttcagg acttccgcgg gctgctctcc ccggaggaaa
     2281 ccgcattcat ggggccggac ggcctcctca atcaggcttt cgttgacggt gcgggcgacg
     2341 ccgctgaggg cgcctacatc accttcggtg gtttgccggc gaacgcgctc gaaggtcccg
     2401 gcgccgtatg ggcggagacg atgcgcgagc ggctcggtca tgagccagac gcctacgcag
     2461 cgtattcgtt cgaggccgcg gttgtggttc ttcaggcgat cgatgcagtt ggcgagaagg
     2521 atcgggcgaa gattctcgag accatgttta acaccaccga cttccgcggc ttgaccaaca
     2581 cctggagctt cacggagacc ggggacccgg ataccgcgac gctgtccgtg aatatcgtga
     2641 aggacggcga gattaccttt ggggaaacga tctctccgcc ggaatagccg tgggctacta
     2701 gcactcaccg gacaggattg gttccggcct ggtgaacgag atgcagcggc gttgatgctc
     2761 gatgccgtca aaggtggagt ggtcagcttt aagaaaccga tcgctccacc cgaataagcc
     2821 acagtgatgt catccggacc gggagcaact cctggtccgg atgccgcgta cacgtagggg
     2881 cggttggcag aggaatgact cccgtggcgg catcttgatg cgggccggga tatgtgtcgg
     2941 atagacgcga tatgcagtgg accctgtttt tccagttctt gattattggc ttggcgactg
     3001 gtgcattgat cgccctgatc gctctgggct atacgctcgt gtacggcatt atcgagctca
     3061 ttaacttcgc ccacggtgaa gtcttcatga tgggggcaat gttcgcggca acggtggtct
     3121 cgctgatggg cgtgacgaac gagtcgacct cactcgtgat tgtcggaacg ttgttgattg
     3181 gactggtcct gtcgatggca ttttgtgccc tgctcaatgc ggggatcgac cggatcgcgt
     3241 accggcggtt gcgccatgcc ccgcgcctgg ctccgttgat tgccgccatc ggtatgagct
     3301 tcattctgca gaacatagcc atctattggc agggcgcgaa ccctattacg ccgcccaata
     3361 tcatcccttc cgaatacaag acctacaaca tattgaaaga gtggccaatt ctgcgcgact
     3421 ggttcgcaga ttcgaccctt cgcttcaggc tgatggatct cgtggtgatg ggcacgacga
     3481 ttcccctgct cattattctc acctggttcg tctaccgcac ccgaatcggg acggcaatga
     3541 gagcaacggc gcaagaccgg gaagcggccg cactgatggg gatcgacatc aatagaacca
     3601 ttgccgttgc cttccttctc ggtggctcca tggccggcgc ggccggtatg atcgccttgt
     3661 tctacaacaa ctctgcccga ttccagatgg gattccaata cggattgtat gcctttacag
     3721 cagcggtcct tggtggaatc gggaatctca acggtgccgt gatcggtggc ttcatgatcg
     3781 ggcttatttg ggcgtacagc gacgggttta tgggtctgta catcggtgga tggggaagcc
     3841 agtggagcaa ttcggtcatc ttcagcatcc tggtactcgt gctggtcttt cgtcccgccg
     3901 gcttgttcgg ccattcagca cctgagaagg tctgagcagg cccgaaggag agaccagtat
     3961 ggccgcatcc attgcctcac gaaccgatgt cttctttcag gaacggcgtg agatcgaacg
     4021 ccagcgactg tggaa
//
LOCUS       DASYWM010000252        15969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_218447, whole genome shotgun sequence.
ACCESSION   DASYWM010000252 DASYWM010000000
VERSION     DASYWM010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 15969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15969
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218447"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VGR29_10295"
     CDS             complement(<1..867)
                     /locus_tag="VGR29_10295"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV2074018.1"
                     /translation="MALGCPVTAPRSARANDLAAAARTGAVIGDGSVTVTGLTFDSRA
                     VQPGDLFAALPGSDFDGHSYIPAAIERGAAVVLAERLIESSVPVILVNDSRAALAPIS
                     AAFYGYPSRELTMIGLTGTDGKTTTSFLVRDLLRAGGHQTGLIGTVGISIGDGTEYHL
                     PHQTTPESNLVQGYLREMVERGTDTAILEATSHGLHMHRLDETVFTIAGVTNITREHL
                     EYHKSVENYRRAKAILIERVAGANGVVVLNADDDGSRSMETAAEGSNVVWYGTKDSRV
                     ELYAANIVVSNTG"
     gene            complement(871..1974)
                     /locus_tag="VGR29_10300"
     CDS             complement(871..1974)
                     /locus_tag="VGR29_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEV2074019.1"
                     /translation="MYSNAEGVHPIVSPQTPSRTEPGAPSIGREHPASLWNAALLRHG
                     GNLLQSWEWGEFKAMHGWSPERISVSGSEGEGFAQVLFRHRGPVSVGYIPRGPVLAGN
                     PDKVWPALRAEIDKVAKRHRAITVIMEADQESGLSGTFTEAGLVPGPAHLQPSRTVKV
                     PLLDSDDEMLKGMHQKTRYNVRLAIRRGVEIEVRDGTDQGALSQYYTLMEDTSERNQF
                     HIHSRKYYEDFLRVFGENAFFVFASWEGHLSAAVIAAAFGDEALYMYGASSTQHRAHG
                     AAFLLQFEVMKWARDRGCKSYDLWGIPQEDPETLRADDNTSIAASRGDDWRGIYRFKT
                     GFGGQIIKYPQTLERRYVPVLPSLARRLGVIRG"
     gene            complement(2010..3083)
                     /locus_tag="VGR29_10305"
     CDS             complement(2010..3083)
                     /locus_tag="VGR29_10305"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV2074020.1"
                     /translation="MPQDHNDESGTPFFSLVDQDGSDNEPFDLQEEAFAELEEAQYAS
                     FDEALEAIRGESLEITSLTGFSDISRNEARRLAGIWRELPVESRVTIADHVHTLGQED
                     LFLDFMRFFRLLLDDDSATVRQIAATGLAPYDDESLIEPLTKHATVDVDDDVRVAAME
                     SLGTFMTLGEFGMLEPKTMKNLRQVLLRSVEDAKAPVRLRAAALAGIAVNSDDNRIQE
                     VIEAFYTSGDPDLRMGAIQAMGRSANPLWIPMLETTLRSADPDERQASAKSLGTYDDP
                     AVVPMLTMLAREDQEMPVRLEAIQALGTVGGRNALHSLQTLREHVSDDEIEAVDAAIA
                     EAEDLIALEEAEPEDEFGFDEDV"
     gene            complement(3067..4965)
                     /locus_tag="VGR29_10310"
     CDS             complement(3067..4965)
                     /locus_tag="VGR29_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074021.1"
                     /translation="MVTETEMPAVSFSGSKQLQETASRAFDVMRARGMFVADNAPIRV
                     NKDDLIAFLTTTGDVSASEVDEALAENANVFLVEANGDGAEVVITTRMGRAPSRDAAT
                     DGHTFANRLMTPEPKPTTPVAPVRERVRVDPSWATFTVPDVGEDEEDDELVAQETLTA
                     VIAAPEVVEDVGAEEPMVAATEVPVAPVEPEFTAAEMPVAPAEPEVIEEPIETPAPAA
                     EVIEDVPAAPAVTDPVEVIEESLAVETAEPVEVAAPVEVAPPVRPIEIPAPVEVAPPA
                     AFDRRIASDARQARFGNQSMPEDRVHRLSRGELRRIREYIEEQEQPLTDETLVQDILG
                     VRPNNPNYEFSLFSVNYRLSKEREFEFVGTSDQRFWATTTLPSIGTDRRKHNDIGTDY
                     RFLAEEVDLAAAPRSVESLDHVVTFYEHTLGLLPYDAAMQKLMPAPMLPDQRNAVFTF
                     EIPQTYTTYLVELRYPTPNRGGFLLGLDDFYADSVVPGAIISVSATENDGHYKVEYLE
                     ADDQEDRLLELDDRRAPRYHFRPTTYSCVVDSDWLINESRFPNLGSEKPLADKVRRRN
                     DAVLKATFERIGMDDGDGGKLASFEDLLAAINIERPFSAAALRAALEADPAVSGDDAN
                     GYVYASGS"
     gene            5422..6642
                     /locus_tag="VGR29_10315"
     CDS             5422..6642
                     /locus_tag="VGR29_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2074022.1"
                     /translation="MGNRPLLTLASGHFTVDMYSGLLPVLYPILTDQFQLDLKTVGLV
                     ALAYSGASSISQPFFGWLADRYGTRFTGVALAWSAILFGTIGFAPTFPILLVMAALAG
                     LGSGAFHPFGAITANSVINDANKNKAMSVYVMGGTLGVSIGPLVGALLFSVLGMRGTA
                     IMILPGVSIAAWLLLAMRSVSIAGTRSKTYIAPRVRHALPLSLITIIVAVMMLRMFPT
                     LGLQNFIPLWYDNMGYGASFYGLLATVVVLSSAFGAIGAGTLADRYGRRTVILATIVL
                     SIPAVWAFAEFPGEWAFVSGAAIGFLSGSTSPLLLVMGQQLMVGRAGVASGLLLGLGF
                     VAGAIGTPVFGHIGDAFGMQNAVRSQVLIMLLSLVVAWFLPSESTIRDILDDRRDAEQ
                     AAARVPSGVTTAAD"
     gene            6929..7894
                     /locus_tag="VGR29_10320"
     CDS             6929..7894
                     /locus_tag="VGR29_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV2074023.1"
                     /translation="MHRLTRVFLVLMLAFSALIPFNIASAQESDDLRFVVVTHGQASD
                     PFWSVVQRGAEQAANDMGVTVEYQAPTTFDMVAMSQLIDAAVASNPDGIVVSIPDASA
                     LSPAIQGALDAGIPVISINSGYETSQELGLMLHVGQTEFEAGFGGGQRMAEEGVTNAI
                     CLIHEAGNAGLEDRCRGFTEGLAESGGTVEQLVVDLNNPTEAQQRVEAALAANAEIDG
                     IMALGPTGAAPALMAIEAAGVSDSVSLATFDLSPEVLQGIQDGTVLFAIDQQQYLQGY
                     LPISLLTLYQRNGNELAEDIIQTGPGFVDASNVDQVVDLIEGGTR"
     gene            7968..9029
                     /locus_tag="VGR29_10325"
     CDS             7968..9029
                     /locus_tag="VGR29_10325"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074024.1"
                     /translation="MPTSATAPATVPKQDDRLKPSSSLRRALLRPEVGATVGALALWV
                     YFVVVAGDSGFLSMRGTASYLEVSAELGILAIAVSLLMIGGEFDLSIGSIIGASGMMV
                     TILSVEYGYNIWFAIVIAFLVSLVIGFINGVIVRWSRLPSFIITLATLFIIRGLTIAF
                     TRMITNRTTLGGLEDVEGYESAKSLFGSNIPVMGVDFPVSIVWWIVLTLIATWVLMRT
                     SFGNWIFGVGGNEQAARNTGVPVHRVKISLFMLTAAAAWLVSVIQVINSTGADVLRGT
                     QQEFFAIIATVIGGTLLSGGYGSAIGAAIGALIFGMVRQGIVYAGVDADWFQVFLGGM
                     VLLAVLINQFVRKRAMGGR"
     gene            9029..9829
                     /locus_tag="VGR29_10330"
     CDS             9029..9829
                     /locus_tag="VGR29_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2074025.1"
                     /translation="MPEAAPLIEVTNISKYFGNVMALQDVSMTVYPGEVHCLLGDNGA
                     GKSTLIKTLSGVHAPDHGEIRIDRQRVRFSSPREALQAGIATAYQDLAMIPLMSISRN
                     FFLGSEPTTGWGPFRRFDVAKADAITRVELDRMGIAVRDTSQPVGTLSGGERQSVAIA
                     RAVYFGARILILDEPTAALGVKEAGVVLRYIAQAREQGLGVIFITHNVHHAWAIGDKF
                     TLLSHGRSEGTFTKDETSREEVISLMAGGAALEELEHELGQHAPPSVR"
     gene            complement(10063..10746)
                     /locus_tag="VGR29_10335"
     CDS             complement(10063..10746)
                     /locus_tag="VGR29_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEV2074026.1"
                     /translation="MSQEPRSEFTAYWLFSRKDDWREQLHGSWDSAVQEFIALIDEPG
                     EGVTVRGVYSTIGLRPDVDMIIWAHAPRLEQVTELAVSLDRTAVGRALRRVQGYIGVG
                     GMSQYDPDHGPAFLKGIPAKRYLSVYPFTKTPDWFLIDFPERRRLMLEHGELGREFPD
                     LLTNTVNSFGIQDQEFIVALEDNDPHQIVKMVQKLRGAEVRKYTALDVPIFLGDRKPP
                     ADVLADIKG"
     gene            10919..12760
                     /gene="uvrC"
                     /locus_tag="VGR29_10340"
     CDS             10919..12760
                     /gene="uvrC"
                     /locus_tag="VGR29_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV2074027.1"
                     /translation="MKETAERPDFSNRLAALAQAPGVYLMKDASDTVIYVGKAISLRN
                     RVRSYFQSQRNKDPKTRELVAHITDFEVIRTDTATEALILENELIKKYLPKYNVMLKD
                     SKTYPYIRITNEEWPRVISTRLIVNDGSQYFGPYTSAGSAYKTINLLNRLFPYRKCDK
                     KITGNDEVCLYYHMHQCTAPCISAVDRPTYMKAIDATAMFLSGRGDEIVTDLEDEMEQ
                     ASEAWNFERAAELRDRLSAVRHILERQKIVTAGGTNADIIAVSQGAGGDAGVQVGFLR
                     NGKIIGSEFFPMQATLNDEPANIVSGFITQFYEDAAVTPPRLILQHQVPETERVMIEE
                     WLADKRGSKVELHVPQRGSQRGLVEMVAKSASENLEQARLKFLSDEMKMTAAMTELAD
                     ALDLPRMPRRIECYDNSNLQGTSPVASMVVFEDGKPAKKEYRRFNIKTVVGADDFASM
                     AEVLTRRFRRAREADEEKEGKWTTLPDLVIVDGGKGQLSAAVAALEDVGMQVPICGLA
                     KENEEIFLPGRPDPILLPRDSQALFLVQRVRDEAHRFAITFHRSTRSKSAFKSRLDEI
                     PGIGPTRKKALIRAFGSVKAIQAASVEELSAVEGINTAVAENIKSHL"
     gene            12807..13484
                     /locus_tag="VGR29_10345"
     CDS             12807..13484
                     /locus_tag="VGR29_10345"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEV2074028.1"
                     /translation="MSYLSLEDKRALETAIATMQGGGVIAFPTDTVYGIGASLSHRDA
                     LTRVYDIKGRSHEKPLPVLVSRADVIDRLANQPDPELLRLASHFWPGPLTIILKAGDT
                     LPTEVIAPDGTFGVRIPDHSIALTIAQHNGGAIAVSSANRSGEPPACHAEEILQALGG
                     EIDVILDGGIAPRSLASTVIRREDDTITVIREGAISSEIIQEAWNEMRVADEIASTAG
                     TMTSPGR"
     gene            13569..14546
                     /locus_tag="VGR29_10350"
     CDS             13569..14546
                     /locus_tag="VGR29_10350"
                     /inference="COORDINATES: protein motif:HMM:NF040134.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074029.1"
                     /translation="MNRDNTNPFSSRRRPADLEYEDDQDYDDYINGSDDDEHKVFGLG
                     ILQADDRQPAYGQGVVISLLSAAALLLLAIIAIGEGDRSDPPIFQQTDTLYWIIAAVV
                     LLLTAGGAQYAERTASAAAYAVGSGRPQGGLATAWAVPFISVFAAIVLTATYHNRWML
                     LFGPLIAFLGTAGALLSRDLLDEADEESSRIASTIHTLVVHAVAFLAFSAIYLNKFDT
                     WVAAPLVGLVGGVLILETLERAGIAPPLRIFYAVLGGWVLAQVMIALNWWPTWGWTGG
                     AVLLAVFYAIAGLMLIRVQREQVRKRDLMEYGAVAGTALLVLALRDVFF"
     gene            14574..14777
                     /locus_tag="VGR29_10355"
     CDS             14574..14777
                     /locus_tag="VGR29_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074030.1"
                     /translation="MSDKIINFDAARRKNRGEELDMHQFLSQIDELEEALETMHRFGI
                     NTREELAELIRTLEEQAASRDDE"
     gene            14803..>15969
                     /locus_tag="VGR29_10360"
     CDS             14803..>15969
                     /locus_tag="VGR29_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV2074031.1"
                     /translation="MDLFEAHRRQQLTSRAPLATRMRPRTLDELVGQEHVVGEGTLLR
                     KAIEADRLSSLILWGPPGAGKTTLARIIATTTRATFESVSAVTSGVADLRRAITEARD
                     RLGMNSQRTVLFIDEIHRFNKAQQDAILPHVEDGTVILIGATTENPSFEVNAPLLSRS
                     RVITLRSLADEDVMAILRRALADEERGLGNDELTITDDGLELLANMANGDARFALNTL
                     EFAATGVGEDRQISADLVREAAQRRAATYDKGGDDHYDTISALHKTIRGSDADAALYW
                     LARMLERGDDPLYVARRLVRFASEDIGLADPAALELAMAAQQAVHFLGMPEGALALAE
                     LTVYLALAPKSNAIYKAYGAVQRDVAESRNDPVPIHLRNAPTRFMKNEGFGKGYK"
BASE COUNT         3135 a         5009 c         4659 g         3166 t
ORIGIN      
        1 gccggtgttg ctgacgacga tgtttgctgc atacaattca acgcgagaat ctttggtgcc
       61 gtaccagacg acattcgagc cttctgccgc tgtttccatg gaccgcgaac cgtcatcatc
      121 ggcgttcaat acgacaacac cattggcgcc agccactctc tcaatgagta tggccttcgc
      181 tcgccggtaa ttctccaccg acttgtggta ttcgagatgc tcacgcgtga tgttcgtgac
      241 acctgcaatc gtgaagaccg tttcgtcgag gcggtgcatg tgaagtccgt gtgaggtggc
      301 ctcaaggatt gccgtgtctg taccgcgctc gaccatctca cgcaggtacc cttgaaccag
      361 atttgactct ggtgtcgtct gatgtggcag gtgatactcc gtgccatccc cgatggagat
      421 gccgaccgta cctataagcc cagtctggtg cccccccgcc cgcagcaaat cccggacaag
      481 aaaactggtg gtcgtcttgc cgtcggtccc tgtcaaaccg atcatcgtca actcgcgact
      541 cggataacca tagaatgcgg cggatatcgg ggcaagggcg gctcttgagt cgttcaccag
      601 gatcaccgga acgctagact cgattaaccg ctcggcgagc accaccgccg ctccgcgctc
      661 aattgcagct ggaatgtagc tgtgcccatc gaaatcgctc cccggaaggg cagcaaagag
      721 atcgccaggc tgcactgcac gggaatcgaa cgtcagaccg gtaaccgtca cgcttccgtc
      781 accgatgaca gcgccagtgc gcgcggcagc cgccagatcg ttcgcacgtg ccgaacgcgg
      841 ggcggtaact ggacatccca gtgccacagc ttacccccgg atgacgccaa gccggcgcgc
      901 gagcgacggc aagaccggga catagcgacg ttccagcgtt tgcggatact taatgatctg
      961 cccaccgaat cctgtcttga accggtagat gccacgccag tcgtcacccc tggacgccgc
     1021 gatcgaggtg ttatcgtccg ctcgtagcgt ctccggatcc tcctgcggaa tcccccacag
     1081 atcgtagctc ttgcaacccc gatcgcgagc ccatttcata acttcaaact ggagcaggaa
     1141 ggcggctccg tgggcgcggt gttgtgtgga cgatgcgcca tacatgtaga gagcctcatc
     1201 accgaaggcg gcagcaatga ctgcggccga gagatgtcct tcccaagacg cgaacacgaa
     1261 gaacgcgttt tcaccgaaga ccctaaggaa atcttcgtag tacttccggg aatgaatgtg
     1321 gaactgattc cgctcggaag tgtcttccat caacgtatag tactggctca gggccccctg
     1381 atcggtgcca tccctgacct caatctcaac gccgcgacgg atggcaagac ggacgttgta
     1441 gcgggtcttc tgatgcattc ccttcagcat ctcatcgtcg ctatcgagca agggaacctt
     1501 cacggtgcgc gacggttgca ggtgagcagg tcccggaaca agacccgcct ctgtaaaggt
     1561 gccactcagc ccagactcct gatcggcctc cataatgacg gtgatcgccc ggtgcctctt
     1621 ggcaaccttg tcaatttccg cacggagcgc cggccatacc ttgtcagggt ttcctgccag
     1681 cacaggccct cgggggatat accccacact caccggcccc cggtgcctga acaggacctg
     1741 ggcgaagcct tcgccttcgg agccggacac gctaatccgc tcgggcgacc agccatgcat
     1801 ggccttgaat tctccccact cccatgactg caggagatta ccgccgtggc gcagcagggc
     1861 cgcattccac aggctcgcag ggtgttcgcg cccaatggac ggagcacccg gctccgtgcg
     1921 ggaaggtgtt tgcggtgaaa ctatggggtg aactccttca gcgtttgaat acatggaccc
     1981 gtcaagaata gagatgaatc aaggggaggc tacacatctt catcgaaacc aaattcgtct
     2041 tccggttcag cctcttccag cgcgatgaga tcctcggcct cggcgatagc agcatcaacc
     2101 gcctcgatct cgtcgtcgct gacatgctcg cgcagtgtct gtaacgagtg caacgcgttc
     2161 cgcccgccaa ccgtgcccaa cgcctgaatg gcctcaagac gtaccggcat ctcctgatcc
     2221 tcacgggcga gcatcgtcaa catcgggacc accgccggat cgtcatacgt gcctagggac
     2281 tttgcggacg cctgccgctc gtctggatcg gcactccgca aggtagtctc aagcattgga
     2341 atccagaggg gattagcgga tcggcccatg gcctggattg ctcccatccg caagtccgga
     2401 tcaccggagg tatagaacgc ctcgatcacc tcctggatcc ggttgtcatc cgagttcacc
     2461 gcgatgcccg caagggctgc cgccctgagc cgcactggtg ccttggcatc ctcgactgat
     2521 ctgagcagaa cctgtcggag gttcttcatc gtcttcggct cgagcatgcc gaactcaccc
     2581 aaggtcataa acgtaccgag cgactccata gccgccacgc ggacatcatc gtcgacatca
     2641 acggtggcgt gcttggttag cggctcgatg agtgattcgt cgtcgtacgg ggcaagcccc
     2701 gtcgctgcaa tctggcgcac cgtagcggag tcgtcatcga gcagtaaccg aaagaaacgc
     2761 atgaaatcaa ggaaaaggtc ttcctgcccc aacgtatgca catggtcggc gatagtgacg
     2821 cgagattcta ccggaagctc gcgccagatg ccggccaaac gccgcgcttc gtttcgggag
     2881 atatcggaga aaccagtgag ggaagttatc tccagagact caccacggat cgcctcaaga
     2941 gcttcatcga agctcgcata ctgcgcctct tccagctccg caaaagcctc ttcctggaga
     3001 tcgaacggct cgttgtccga gccgtcctga tccaccagcg agaagaaggg cgtaccgctc
     3061 tcgtcgttat gatcctgagg catagacata cccgtttgca tcatcgccgg agacggcggg
     3121 atcggcctcc aacgccgccc gtagcgctgc tgccgagaac gggcgctcga tattgatcgc
     3181 cgcgagcaga tcctcgaacg aggccagctt gccaccatcg ccatcgtcca tgccaatgcg
     3241 ctcgaacgtc gccttaagaa cggcatcgtt gcggcgtcga actttgtcag caagtggctt
     3301 ctcggaaccg agattgggga agcgactctc gttgatcaac cagtccgagt cgaccacgca
     3361 ggagtaggtg gtggggcgga agtgatagcg cggcgcacgt ctatcgtcca gctccagtag
     3421 ccggtcttcc tgatcgtcag cttcgagata ctccaccttg tagtgaccgt cgttctcggt
     3481 ggcgcttacc gagatgatgg cacccggcac gacgctatcc gcgtagaagt cgtcgagccc
     3541 gagcaggaac ccaccgcggt tcggcgtcgg atagcgaagc tccaccagat aggtggtata
     3601 ggtctgcgga atctcgaagg taaagacggc gttgcgctgg tccggaagca ttggcgccgg
     3661 catcagcttc tgcatggccg catcatatgg gagcaggccg agcgtgtgct cgtagaaagt
     3721 caccacgtgg tcgagggact caacggagcg cggcgcggct gccaggtcta cctcctcggc
     3781 caggaagcgg tagtccgtcc cgatatcgtt gtgcttgcgg cggtcggtcc cgatagacgg
     3841 cagcgtggtg gtcgcccaga aacgctgatc gctggtacca acgaactcga actcccgctc
     3901 ctttgagagg cggtagttca ctgagaagag gctgaactca tagttcgggt tgttcggtcg
     3961 cacaccgagg atatcctgga ccagcgtttc gtcggtgagt ggctgctcct gctcctcgat
     4021 gtactcccgt atacggcgaa gttcaccacg actgaggcgg tgaactcgat cctcaggcat
     4081 cgactggttg ccgaagcggg cctgtcgggc gtcgctggcg attctcctgt cgaaggccgc
     4141 gggcggcgcg acctccaccg gcgctggaat ctcgatcggc ctcacggggg gagcaacttc
     4201 gaccggcgcc gcaacctcga caggctcagc agtctcaacg gccagagact cttcaatgac
     4261 ctcaaccgga tctgtgactg ctggagctgc agggacgtct tcgatgactt ccgcggccgg
     4321 tgctggagtc tcgatcggct cctcaatcac ctcgggctca gcaggcgcaa ccggcatctc
     4381 ggccgcggtg aactcgggct caacaggtgc gacaggcact tccgtcgcgg caaccatggg
     4441 ctcctcggct cctacatcct ctacaacctc aggcgcggca ataacggccg tcagagtctc
     4501 ctgggcgacg agctcatcgt cctcttcgtc ctcgccaaca tccgggactg taaaggtcgc
     4561 ccacgaagga tcgacacgga cgcgttcgcg caccggggcg accggcgttg tcggcttggg
     4621 ctcgggggtc atcaggcggt tggcgaacgt atgcccgtct gtcgcggcat ccctggacgg
     4681 agctcgtccc atccgcgtcg taatcacgac ctcagcgccg tcaccgttcg cttcgaccag
     4741 gaagacattc gcgttttccg ccaatgcctc gtctacctca gacgcactaa catcgcccgt
     4801 agtggtgagg aaggcaatga gatcgtcctt gttcacgcgg attggcgcat tgtcggccac
     4861 gaacatgcca cgcgcgcgca tcacatcgaa tgcccgggac gcggtctcct gaagttgctt
     4921 cgatccggaa aaggagacgg ctggcatctc ggtttcggtc accatatttg gatctgctcc
     4981 tcaatacatt ggatgatgat tgtcccgtgc tgcacgggac gatgcgggaa gtcctctcaa
     5041 gaatcagtaa gtcaggatgt tgtcggaggg tgcgaaccat atgtctgtgt ggtgagcggc
     5101 gatttctggc tgcgaacaaa atcttgatca ctggcgcgat atcattgtgg tatcaacggt
     5161 acccgccact ctggtcctga tcagggtctt aatctcctca agacaccgct gtctactgat
     5221 ctcggggcac agtgaatgcg acgacagagc ccgtcgcaac cgaaggatta tagtctattt
     5281 gcgtccgaga ggcggtgaac cattgaccgc cgatcgctcc gcatgtagaa attgtccttg
     5341 tcacctcacg atttcgggtt cagatcatcc ctacagaaat ggctcatctc aattatggca
     5401 gcgagttcaa ggcgagctat tttgggcaac cgccccttgc tgacgctcgc ttccggccat
     5461 ttcacggtcg acatgtacag cggtctcctc ccggtcctct acccaattct cacggatcag
     5521 ttccagctcg acctgaagac ggtcggcctc gtagcgctcg cctattccgg tgcgtcatcg
     5581 atctcccaac cttttttcgg atggctcgcg gatcggtacg gaacacgctt caccggtgtc
     5641 gctctcgcct ggtcagccat cctcttcggc accatcggct tcgcaccgac ttttccgatc
     5701 ctgctggtca tggctgcgct cgcagggctc ggctcagggg cgttccatcc cttcggtgcc
     5761 atcacggcca actcggtcat caacgatgcg aacaagaaca aggcgatgtc cgtctatgtg
     5821 atgggcggaa cgcttggagt ctcgatcggc ccgcttgtcg gcgccttact cttctcggtc
     5881 ctggggatgc gcggtacggc catcatgatc ttgcctggtg tctccatcgc ggcctggctc
     5941 ctgttggcca tgcgctccgt ctcgattgcc ggtacccgta gcaagaccta tatcgccccg
     6001 agggtcaggc atgcactgcc actttccctg atcacaatca tcgtcgccgt catgatgctg
     6061 cggatgttcc ccacgcttgg tctccagaac ttcatcccgc tctggtacga caacatgggc
     6121 tatggcgcat cgttttacgg cctcctcgcc actgtcgtcg tcttgtccag cgcgtttggc
     6181 gcgatcggtg ccggcacgct ggccgacaga tacggcaggc gtacggtgat cctggcgacc
     6241 atcgtcctga gcatccccgc cgtctgggcc ttcgcggaat tcccgggcga gtgggctttc
     6301 gtttccggag ccgccatagg cttcctgtcc ggctcgacat ctcccttgct gctggtcatg
     6361 ggccaacagc tgatggtcgg acgcgccggg gtggcctccg gactcctcct cgggctcggc
     6421 ttcgtcgctg gcgcgatcgg cacgccggta ttcgggcaca tcggcgatgc gttcgggatg
     6481 cagaacgcag tgcgatccca ggtcctgatc atgctccttt ccctcgtcgt tgcgtggttt
     6541 ctcccgtcgg aaagcacgat ccgcgatatt ctggacgatc gccgggacgc tgagcaggcg
     6601 gctgcacggg ttccctccgg cgttacaacc gcggcagact aggtctcaac tgactcaaga
     6661 aatgacatca ctcctgcccc aaacccggtt gcccggggtt gcatcggggt aagcgagcgg
     6721 tatcatggat aaatatcgat aaagacgaga tgtacttgag agaccgtccg gagccggccg
     6781 atcctggacc gcgccacaga ttctccattg cggagagaga aaggatgggc cacttcctgc
     6841 aggagtcagg gagccttttc acatagccct gcaacgctct cccgcgtgag cggctgagcc
     6901 atttgttgac ccagtgagga gaaccgaaat gcacaggctt acccgcgtct tcctcgtgct
     6961 catgcttgcc ttcagcgccc tcatcccctt caacatcgcg agcgcccagg agtccgatga
     7021 tctccgcttc gtggtcgtca cgcacggcca ggcatccgac cccttctggt cggtcgtcca
     7081 aaggggcgcc gagcaagcgg cgaatgatat gggtgttacg gtcgaatacc aggctccgac
     7141 aaccttcgac atggtagcca tgtcacagct catcgatgct gctgtcgcat cgaatcccga
     7201 cggaattgtc gtctcaatcc ccgacgcctc cgccctttcg ccggcgatcc agggcgcgct
     7261 tgatgccggc atccccgtga tttccatcaa ctccggctat gaaacctccc aggagctcgg
     7321 tctgatgctc catgtcggac aaaccgagtt cgaggctggt ttcggtggcg gacagcgcat
     7381 ggccgaggaa ggcgttacca acgcgatctg cctcattcac gaagcgggca acgccggtct
     7441 tgaggaccgc tgccgcggct tcaccgaagg actcgccgaa agtggcggca cagtggaaca
     7501 gctggttgtt gacctcaaca acccaacaga agcacaacaa cgcgtggaag cggcacttgc
     7561 ggccaacgcc gagatcgacg gcatcatggc gctcggccca accggagccg ctcctgccct
     7621 gatggctatc gaagccgctg gtgtctcgga ttcagtcagt ctcgccacct ttgacctctc
     7681 gccggaggtc cttcagggaa tccaggacgg cactgtcctc tttgcaatcg accagcagca
     7741 atacctgcag ggttatctcc cgatctcgct gctgactctc taccagcgca acggcaacga
     7801 gctggctgag gacatcatcc agacgggccc cggcttcgtc gatgccagca atgtcgatca
     7861 ggtcgttgac ctcattgagg gcggcacccg gtagaccgtc ctgtaggtgg cggaggttct
     7921 tccgccacct atatcgttgc cttcaccccg ccctggagag catcgcaatg ccgacatcgg
     7981 ccactgcccc agccactgtg ccgaaacagg acgaccgtct caagccctcc agctccctgc
     8041 gccgcgcgct gctccgtccg gaggttggcg ccacggtcgg ggctcttgcg ctctgggtct
     8101 acttcgtcgt ggtcgctggg gacagcggct tcctctccat gcgcggcacc gccagttacc
     8161 tggaggtctc cgccgaactt ggcatcctgg ctatcgccgt gtccctgctg atgatcggag
     8221 gtgagttcga cctgtcgatc ggctcgatca tcggtgcctc tggcatgatg gtcacgattc
     8281 tctccgtgga atatggctat aacatctggt ttgcgattgt gatcgcgttc ctggtgtcgc
     8341 tggtcatcgg ctttatcaac ggcgtgatcg tccgctggtc gcggctgccg tcgttcatca
     8401 tcacgctcgc cacgctgttc atcattcgcg gattaaccat cgcctttaca cggatgatca
     8461 ccaatcgcac cacactcggg ggactggagg acgttgaagg gtacgagtcg gccaaatcac
     8521 ttttcggatc taacatcccg gtcatgggcg ttgatttccc ggtttcgatt gtctggtgga
     8581 tcgttctgac gctcattgct acttgggtgc tgatgcgaac ctccttcggt aactggatct
     8641 tcggtgtcgg cggcaacgag caggccgccc ggaacaccgg cgtccccgta catcgcgtca
     8701 agatctcgct cttcatgctc acggccgcgg cagcctggct ggtatcggtc atccaggtaa
     8761 tcaactcgac cggcgcggat gtccttcgtg gcacccagca ggagtttttc gccattatcg
     8821 cgacagttat tggaggcaca ttgctctcgg ggggctatgg ctccgctatt ggtgccgcta
     8881 tcggggcgct gatctttggg atggtcaggc aaggaattgt atacgccggt gttgacgcag
     8941 actggttcca ggtcttcctt ggaggcatgg tcctgctcgc cgtactgatc aaccagttcg
     9001 tgcgcaagcg cgcgatggga ggccgctgat gccggaggca gcaccgctga tagaagtgac
     9061 caacatctcc aagtacttcg ggaatgtgat ggcgctccag gatgtctcga tgacggtcta
     9121 ccccggcgag gtgcactgcc tcctcggcga caacggcgct ggaaaatcga cgctaatcaa
     9181 gacgctgagc ggtgtgcatg cgccagacca tggcgagatc cggatcgatc gacaacgcgt
     9241 ccggttctca tcgcctcgcg aggcgctcca ggccggcatc gctaccgcgt accaggatct
     9301 ggcgatgatt ccgctgatga gcatctcacg aaacttcttc ctcggctcgg aaccgacgac
     9361 cggctggggg ccgttccggc gcttcgacgt tgccaaggcc gacgcgatta cccgggtcga
     9421 gctggaccgg atgggcatcg ccgtccgcga tacatcgcag ccggtcggga ccctctctgg
     9481 cggcgagcgg caatccgtcg cgatcgctcg cgcagtgtac ttcggtgcca gaattttgat
     9541 tctcgacgag ccgaccgcag cactcggcgt caaggaggcc ggggttgtcc tgcgctacat
     9601 cgcgcaggcg cgagagcagg gcctcggtgt catcttcatt acccacaacg ttcaccatgc
     9661 gtgggcgatc ggcgacaagt tcaccctgtt gagccatggt cgctccgagg ggaccttcac
     9721 caaggacgag acctcccggg aagaggtcat ctcgctcatg gccggaggcg ccgcactgga
     9781 ggaactggag catgagctcg gccaacacgc gccgccgtcc gtacgatagc catttcactc
     9841 aacccgatca cgcaattgcg cacttgctgc ccaaatgtca ccggcatccg gggcaacgac
     9901 cggcccgtgt gcggaccgag ctcacatcaa acccggctgg tcactgtcac ccacttccgg
     9961 gcaatagcgt cagccacacg ctcgtgttaa gacgacacag ccgaagggca gccgtcccga
    10021 cacaagtcgg ctgctgctac gcgctgggaa ccgagcgctg gtctagccct tgatgtcggc
    10081 cagaacgtcg gcggggggct tgcgatcacc caggaagatc gggacatcga gcgccgtgta
    10141 tttgcgcacc tcggcacccc ggagcttctg caccatcttc acaatctggt gtggatcgtt
    10201 gtcctcgagc gcgacgatga actcctggtc ctgaatacca aacgagttga cagtgttggt
    10261 cagcaggtca gggaactccc gtcccaactc accatgttcc agcatcagcc ggcgccgctc
    10321 ggggaaatcg atcaggaacc agtccggagt cttggtaaat ggatagacgc tgagataccg
    10381 cttcgccggg atgcccttga gaaacgcggg gccatggtct ggatcgtact gcgacattcc
    10441 gccgactccg atgtacccct gaacccgcct gagcgcacga ccgaccgccg tcctatccaa
    10501 actcaccgca agctccgtga cctgctcgag gcgcggggca tgggcccaga tgatcatgtc
    10561 cacatcgggc cgcaatccga tcgtcgagta gacaccacga actgtgacgc cctcacccgg
    10621 ttcgtcgatc agtgcgatga attcctggac ggcggaatcc caggagccgt ggagttgctc
    10681 gcgccagtcg tccttgcggg agaatagcca gtatgcggtg aattcagaac gcggctcttg
    10741 cgacatggtg actccttgca tgcgggatgg cgctacccgc taagtactag gtagcgacct
    10801 cgatgcttcc aatgatgcgc cgtcgatgct gctggcgcca ctccctgcga gcgcccatgc
    10861 acgaccgatc ttccccgaac ttcccggacg agagccgttt cccggtatac ttcctcacat
    10921 gaaggagacc gctgagcgcc ccgatttttc caaccgtctc gctgctcttg cgcaggcccc
    10981 gggcgtctat cttatgaagg acgcgagcga tacggtcatc tacgtcggca aggcgatctc
    11041 gctccggaac cgcgtgcgct cctacttcca gtcccagcgc aacaaggatc cgaagacccg
    11101 cgagttggtc gcgcatatca ccgactttga ggtgatccgt accgatacgg caaccgaagc
    11161 cctgatcctc gaaaacgagc tgatcaagaa gtacctgccg aaatataacg tcatgctgaa
    11221 agacagcaag acgtacccct acatccggat taccaatgag gaatggccac gcgtcattag
    11281 cacgcgcctc attgtcaacg acggcagcca gtattttggc ccttatacct cggcaggttc
    11341 cgcctacaag accatcaacc tgctcaatcg gctcttcccc taccggaaat gcgacaagaa
    11401 gatcaccgga aacgacgagg tctgcctcta ctaccacatg caccagtgca ccgcgccgtg
    11461 tatcagcgcc gtcgaccgtc ccacctacat gaaggcgatc gacgccacag cgatgtttct
    11521 ctccgggcgt ggtgatgaga tcgtcaccga cctggaggat gagatggagc aggcctcgga
    11581 agcgtggaat tttgaacgtg ccgccgagtt acgagatcgc ctcagcgccg tccggcacat
    11641 cctcgaacgc cagaagattg tcaccgctgg cgggacaaac gccgatatca ttgctgtctc
    11701 acagggagcg ggtggggatg ccggagtgca ggtcggattt ctccgcaatg ggaagatcat
    11761 tggctccgaa ttcttcccga tgcaggcaac acttaatgac gaaccggcaa acatcgtctc
    11821 cggcttcatc acgcagtttt acgaggatgc tgcagtcaca cctccccgcc tgatccttca
    11881 gcaccaggtg ccggagacgg agcgggtgat gatcgaggag tggcttgcgg acaagcgcgg
    11941 gagtaaggta gagcttcacg tgccccagcg cggcagccag cgtgggcttg tggagatggt
    12001 cgccaagagc gcctcggaga atctcgaaca ggcgcgcctg aagttcctct cggatgagat
    12061 gaagatgacc gcggcgatga cggagctcgc ggatgcgctc gacctgccgc ggatgccccg
    12121 ccggatagag tgctacgaca actccaacct gcagggaaca tcgccggtcg catcaatggt
    12181 tgtctttgag gacggtaaac ccgcgaagaa ggagtaccgt cgcttcaaca tcaaaaccgt
    12241 cgttggggcc gacgacttcg cctccatggc cgaggtgctc acccgtcgct tccgccgcgc
    12301 aagggaggcg gacgaggaga aggaaggcaa atggacaacc cttcccgacc tcgtaatcgt
    12361 agatggtggc aagggccagc tgagcgccgc ggtggcagcg cttgaggatg tcggcatgca
    12421 ggtgccgatc tgtggccttg ccaaggagaa tgaggagata ttcctgcccg gtcgtccgga
    12481 tccaatcctg ctgccccgcg actctcaggc actcttcctc gtccagcggg tccgggacga
    12541 ggctcaccga ttcgcgatca cctttcaccg ctcgacccgc tccaagtccg cgttcaaatc
    12601 ccgattggat gagattccgg gcatcggccc tacccgcaag aaggcgctaa tccgggcctt
    12661 tggttcggtc aaggccatcc aggcggcatc agtcgaggag ctctcagccg tcgagggcat
    12721 caacaccgca gtcgccgaga atatcaagtc gcacctgtaa tacgaaacac tgcaaaggtg
    12781 cagtgacaag cagggggtca cgacccatgt catacctctc actggaagac aaacgggcac
    12841 tcgagacagc aatcgcgacg atgcagggag gaggcgtaat cgccttcccg acggacacgg
    12901 tctacggcat cggggcatcg ctatctcatc gggacgctct cacccgtgtc tacgacatca
    12961 agggccggtc ccatgagaag ccgctaccgg tactggtttc ccgcgcggac gtgatcgaca
    13021 gactggccaa ccagccggat cccgaattgc tccggcttgc ttcgcacttc tggccagggc
    13081 cgctcacgat catcctgaag gctggcgaca cccttccaac ggaagtgatc gcaccggacg
    13141 gcacctttgg tgtccgcatc ccggatcact cgatcgcgct gacaattgcc cagcacaacg
    13201 ggggcgccat cgccgtgagc agtgcaaaca ggtccgggga gccgccggcc tgccatgcgg
    13261 aagaaatcct ccaggcgctc ggcggagaaa ttgatgtgat tctcgatgga ggcatcgcgc
    13321 cgcgtagtct cgcgtccacc gtgatcaggc gtgaggatga taccatcacc gtcatacggg
    13381 aaggcgctat cagcagcgag atcatccagg aagcctggaa cgagatgcgg gttgctgacg
    13441 agatagcgtc gacggcagga accatgacgt cgcccggcag ataaactgcg cgaggcaccg
    13501 ggtagtttcg cgtacttacg cgggttgagc gaaaacaccg ttaccgttag agagatcagg
    13561 acgaacgcat gaatcgggat aatacgaacc ccttctcgtc gaggcggcga ccggctgacc
    13621 tcgaatatga agatgatcag gactacgacg actatatcaa cgggtccgac gacgacgagc
    13681 acaaggtctt cggcctgggc atcctccagg cggatgaccg ccagccagcc tacggccagg
    13741 gagtggtcat ctccctgctg agtgccgctg ctctcctgct gctggcgatc atcgcgattg
    13801 gcgagggtga cagaagtgac cccccgatct tccagcaaac cgacaccctg tactggatca
    13861 tcgctgcggt cgtgctactc ctgacagccg gaggtgcaca gtatgcggag cgcaccgcgt
    13921 cggccgcggc ttacgccgtt ggatccggcc gcccacaagg cggtttggct acggcgtggg
    13981 cggtgccgtt catctccgtc tttgccgcga tcgtgctcac cgcgacctat cacaaccggt
    14041 ggatgctgct cttcggtccg cttatcgcct ttctgggcac ggccggagca ctgctatcgc
    14101 gcgacttgct tgatgaagcc gatgaagaat caagtcgtat tgcgtcgacc attcatacgc
    14161 ttgtagtcca tgcggtcgcc tttctcgcct tcagcgccat ctacctcaac aagttcgata
    14221 catgggttgc agcgccactt gtcggtctcg tcgggggcgt gctgattctg gaaacgctgg
    14281 agcgtgccgg tattgctccc ccgttgcgca tcttctatgc ggtgctcggc ggttgggtgc
    14341 ttgcccaggt catgatcgcc ctcaactggt ggccaacttg gggctggacc ggtggtgccg
    14401 tgttgcttgc cgtcttttac gccatcgccg ggctgatgct cattcgcgtc cagcgcgaac
    14461 aggtgcgaaa gcgtgatctc atggagtacg gcgccgttgc cgggacagcg ctgctcgtgc
    14521 ttgccctgcg cgacgtcttc ttctagatca tcacccgaag gagatgcagc cgcatgtcag
    14581 acaagatcat taacttcgat gctgccaggc ggaagaaccg cggagaggaa ctggacatgc
    14641 accagttcct ctcccagatc gacgagcttg aggaagccct cgagacaatg cacaggttcg
    14701 gaatcaacac acgcgaggag cttgccgagc tcatccgaac actggaggag caggccgcat
    14761 ccagggacga cgagtaaccg caccccttac actggcaggt acatggatct cttcgaagcc
    14821 caccggcggc agcaactcac gagccgtgca cctcttgcca cccggatgcg tccacggacg
    14881 ctcgacgaac ttgttggaca ggagcatgtc gttggagaag gaacgctcct gcggaaagcc
    14941 atcgaggcag accggctctc ctcgctgatc ctgtggggtc ctcccggggc cggcaagacg
    15001 acgctcgccc gcatcatcgc gacgacgacg cgcgcgacct tcgagtcggt gagcgcagtc
    15061 acttccggcg tcgcggacct gcgcagggca atcaccgagg caagggaccg ccttggcatg
    15121 aacagccaac gcactgtgct cttcatcgat gagatacatc gcttcaacaa ggcccagcag
    15181 gacgcgatat tgcctcacgt tgaggacggc acggtcatcc tgatcggcgc cacaaccgag
    15241 aacccctcgt ttgaagtcaa cgccccactg ctctcccgct cccgcgttat tacgctccgc
    15301 tcccttgccg acgaagacgt catggccatc ctccgacggg cattggcgga cgaggagcgg
    15361 ggcctgggca acgacgaact gacgatcacc gacgatggcc tggaactgct tgcgaacatg
    15421 gcgaatggcg atgcccggtt cgcactcaat acattggaat tcgctgccac cggcgtaggc
    15481 gaggatcggc agatcagtgc cgatctcgtg cgcgaggccg cccagcgccg tgccgcaacg
    15541 tatgacaaag gcggggatga ccactacgac accatctccg cactgcacaa gacaattcgc
    15601 ggctccgatg cggatgccgc cctctactgg cttgcacgga tgctcgaacg cggtgacgat
    15661 ccactctatg ttgcccgcag gctggtcagg tttgcctcgg aggatattgg cttggccgac
    15721 cctgctgccc tggagctggc catggcggcc cagcaagcag tccacttcct tgggatgccg
    15781 gagggtgcgc tggcgctggc ggagctcacc gtctatttgg cgttggctcc aaagagcaac
    15841 gccatctaca aggcctatgg ggccgtccag agggatgtcg cggagagccg caacgatccc
    15901 gttccaatac acctgcgcaa cgcccccacc cggttcatga aaaacgaggg gttcggcaag
    15961 ggatacaag
//
LOCUS       DASYWM010000253         6581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_536914, whole genome shotgun sequence.
ACCESSION   DASYWM010000253 DASYWM010000000
VERSION     DASYWM010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536914"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /gene="hslV"
                     /locus_tag="VGR29_10365"
     CDS             complement(<1..498)
                     /gene="hslV"
                     /locus_tag="VGR29_10365"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEV2074032.1"
                     /translation="MNQERNWNPHATTILGVKRSGEVALAGDGQVTIGDVVLKHNARK
                     LRTLHDGQVLAGFAGAVADALTLFEKFESQLQAWDGNLRRAAVELAKEWRSDRYLRRL
                     EAQLLVADGESLLLISGEGDVIEPDDGVVAIGSGAPFATAAAKALITNTDMPAEAIVR
                     ESMRVT"
     gene            complement(650..2893)
                     /gene="topA"
                     /locus_tag="VGR29_10370"
     CDS             complement(650..2893)
                     /gene="topA"
                     /locus_tag="VGR29_10370"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV2074033.1"
                     /translation="MATVNADEKPAKSATASKKSTTRKPAVSKKAPSAGRGKLVIVES
                     PAKAKTIGRYLGRGFSVKASMGHIRDLPKSTLGVDVDADFEPKYLIPRDKSKVVKELK
                     ESVQGAREIYLATDPDREGEAIAWHLVAATEAENKTVHRVVFHEITPQAVMAAIENPR
                     EIDMDLVDAQQARRILDRLVGYGISPLLWKKVKRGLSAGRVQSAALRIVVDREREIEQ
                     FRPVEYWSLDADLAKITGKRPTKKDQFRASIHQVDGKKAELTTGEQAHGIVKALEGAS
                     WQVSSVVRRETKRNAQAPFTTSTLQQEASRKLGFSVRRTMQIAQELYEGVDLGTEGTQ
                     GLITYMRTDSVNISTGVQHQTRDLIANMFGGEFVPEKLPFYRKKQKGAQEAHEAIRPT
                     DPGRAPMAIKSRLSAPQFKLYNLVWQRFMASQMAPARLDNTRVDVVAGAEGEIRTGKK
                     PYVFRATGSIVTFPGWMAVYQQGRDEGDTDELDRGALPDVSEGEPLDLQRLLPEQHFT
                     QPPPRFTEATLVKALEEQGIGRPSTYVPTIGTLQARNYVTVEEKKLVPTELGLVVNDL
                     LVEHFPDLFNIGFTSQLEGELDDIASGEREWIPTLHEFYQPFTRTLQNAEKTMERVKI
                     RDEPTDEVCEKCGSPMVIKLGRFGKFLACSAFPECRNSKPLLTRIGVPCPTCREGEVV
                     ERRSKKGRKFYGCKRFPECDFVSWNKPVNEGCPECGSYMVEVGRGSRLKCSSCTYQGA
                     PLARAGD"
     gene            complement(3110..3325)
                     /locus_tag="VGR29_10375"
     CDS             complement(3110..3325)
                     /locus_tag="VGR29_10375"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074034.1"
                     /translation="MQKCEVAAAQQALPMSDADRRLSAVITSEPQHIDDLALLCGTTV
                     SQISGRLMMLELQGMVRNTGVQHYARI"
     gene            complement(3419..4347)
                     /gene="dprA"
                     /locus_tag="VGR29_10380"
                     /pseudo
     CDS             complement(3419..4347)
                     /gene="dprA"
                     /locus_tag="VGR29_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
     gene            4346..5116
                     /locus_tag="VGR29_10385"
     CDS             4346..5116
                     /locus_tag="VGR29_10385"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEV2074035.1"
                     /translation="MNPTNRVKEKLSAGERVFGCFIPLASPEVVEVCALTGFDFALID
                     AEHGPISPETAYPMVLAAESRGIEAFARVGQADKQMVLKFLDIGVSGIMAPQVNTIEQ
                     ATAAVAGTKYFPTGNRGLAGGRMFDFGLTAHLSALVEPLNDRVLTMIQFEHVDALKDL
                     DAILALEHLDVLFVGPNDLAQSLGYPGQPGHPEVTRVADEVVARAKGAGVKTGTVAFT
                     VDQVHNVFGRGFDMAVANAVGLLAGGAKAYIEGGRQAP"
     gene            complement(5213..5458)
                     /locus_tag="VGR29_10390"
     CDS             complement(5213..5458)
                     /locus_tag="VGR29_10390"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV2074036.1"
                     /translation="MEIIYYLIIGLIAGAIAGYLVPGRTPGGLIGTIVVGIVGAILGG
                     WIFNSLLDLGVGGGIIGSIIVAVIGAVIILFALRKMT"
     gene            5859..>6581
                     /locus_tag="VGR29_10395"
     CDS             5859..>6581
                     /locus_tag="VGR29_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2074037.1"
                     /translation="MAAEPQTTTSAAVRQRSGEPLLEVKNLKTQFFTQDGVVRAVDDV
                     SFYIMPGETLGVVGESGSGKSMTGLSLMRLIPNPPGKIVNGEVIFHGRDVLTMSEDEV
                     RGIRGNDIAMIFQDPMTSLNPVLTINRQISESLILHMGMNKNQAKQRAIELLDMVGIP
                     NADERVDQYPHQFSGGMRQRVMIAMALSCNPQLLIADEPTTALDVTIQAQILDLMTTL
                     QSDTGTGVLLITHSMGVVAGMAD"
BASE COUNT         1321 a         2023 c         1879 g         1358 t
ORIGIN      
        1 cgtcacccgc atcgattcgc gaacgatcgc ttcagccggc atatccgtgt tcgtgatcag
       61 ggccttcgcc gcggcagtgg cgaagggagc accagagcca atagcaacga ctccatcgtc
      121 cggctcaatc acatcaccct caccggaaat cagaaggagc gactctccat ccgcgacaag
      181 cagctgcgcc tccaatctgc gcaggtagcg gtcgctccgc cactccttgg ccagctcaac
      241 tgctgcgcga cggaggttgc catcccatgc ctgaagctgc gactcaaact tctcaaacaa
      301 agtgagggca tcggccactg caccggcaaa accggcaagc acctggccat catggagtgt
      361 tcgcagcttc cgcgcattgt gcttcaggac aacatcgcca atcgtcactt gcccatcccc
      421 tgcaagtgca acctcgccgc tgcgctttac gccaaggatc gtggtggcgt gcggattcca
      481 gttccgttcc tgattcataa cttccttcat catcgacttc gtatccaagg cgtgatgtgc
      541 aagatcacag cgcgtgccac aggcgtcagt gtaccgccaa cgagccccgg ttcctgcagg
      601 aaccggggct cgttaaagcc ttctgtctac tggcccgtgg ccagtgacat taatcgcccg
      661 ctcttgcgag cggcgcaccc tggtatgtgc aactggaaca cttcaggcga ctgccacggc
      721 ctacttcgac catatatgac ccgcattccg ggcacccctc attgaccggc ttgttccagg
      781 agacaaagtc gcattcggga aagcgcttgc agccatagaa ttttcgaccc ttcttcgagc
      841 gtcgctccac aacttcaccc tcccggcacg tcgggcacgg cacgccaatg cgagtcagga
      901 gcggcttgga gtttcggcat tccggaaagg cgctgcaggc aaggaacttc ccaaaccggc
      961 cgagcttgat gaccatcggg ctgccacact tctcgcagac ctcgtccgtc ggctcgtccc
     1021 tgatcttcac acgttccatc gtcttctcgg cgttctgcaa cgtccgggtg aagggctgat
     1081 agaactcgtg aagcgtggga atccactccc gctctccgga ggcgatgtcg tcaagttcac
     1141 cttcgagctg cgacgtaaag ccgatattga acagatccgg gaaatgctcc accagcaggt
     1201 cgttcacgac gaggcccagc tcagtgggca ccagcttttt ctcttcaacc gtcacataat
     1261 tccgggcctg cagcgtcccg atcgtcggca cgtaggtact tggacgacca atcccctgct
     1321 cttcaagcgc cttaacgagg gtcgcttccg tgaagcgcgg aggtggttgc gtaaagtgct
     1381 gctcaggcag gagacgttgc aggtcaaggg gctcaccctc ggacacatct ggcagcgctc
     1441 cccggtcgag ctcgtccgtg tctccctcgt ctcgtccttg ttgatagaca gccatccatc
     1501 cggggaacgt cacgatcgaa ccggtggcgc gaaaaacgta aggtttcttg ccggtcctaa
     1561 tctcaccctc tgctccggca acgacatcga cgcgggtgtt gtcgagcctt gcgggtgcca
     1621 tctggctggc catgaaccgt tgccagacga ggttataaag cttgaactgc ggcgcgctca
     1681 ggcgtgactt aatcgccata ggcgcacgtc caggatccgt cgggcggatc gcctcgtgcg
     1741 cctcctgcgc ccccttttgc ttcttccggt agaagggaag tttctccggg acgaattcgc
     1801 cgccaaacat attggctatg agatcgcgtg tttggtgctg aacgcctgtc gaaatattga
     1861 cggagtcggt acgcatgtag gtgatcaaac cctgcgtacc ttccgtacca aggtcgaccc
     1921 cctcgtagag ttcctgcgcg atctgcattg tgcgacgtac cgagaacccg agtttgcgcg
     1981 acgcttcctg ctgtagcgtg ctggtggtga aaggcgcctg ggcattccgc ttggtctcac
     2041 gccgaaccac cgaggaaacc tgccacgatg ctccttctag cgcctttacg atcccatgcg
     2101 cctgttcacc agtggtgagc tccgccttct ttccatcgac ttggtggatc gaagcacgga
     2161 actggtcctt cttggttggt cgcttgcctg taatctttgc caggtccgcg tccaacgacc
     2221 agtactcgac cggccggaac tgctcgatct ctcgttcgcg atcgaccacg atccgcaagg
     2281 cggcggattg gacccgtcca gccgagaggc cacgcttgac cttcttccat agcagcgggc
     2341 tgattccgta tcccaccagg cggtcaagga tcctccgggc ctgttgggca tccacaagat
     2401 ccatgtcgat ttcacgagga ttctcgatcg ccgccatgac cgcctgaggc gtaatctcgt
     2461 gaaagacaac acggtgtacg gtcttgttct ctgcctccgt agccgccacc aggtgccagg
     2521 caatggcttc gccctcgcga tcagggtcgg tcgcaaggta gatctccctg gcgccctgaa
     2581 cgctttcctt cagttcctta acgaccttcg acttgtcgcg tggaatgagg tacttcggct
     2641 caaagtccgc gtcgacatcc accccaagcg tgctcttggg caggtcacga atgtgaccca
     2701 tcgatgcctt caccgagaag ccacggccaa gataccggcc gatcgtcttg gccttcgcgg
     2761 gtgattcgac aatcaccaat ttcccacgac cagcggatgg cgctttcttg ctgacggcag
     2821 gcttgcgagt cgtcgacttc ttgctcgcag ttgccgattt ggcgggtttt tcatccgcat
     2881 tcacggttgc catgtactgt atattccttg atattcgccc gtaggcgaaa tgaaatcccc
     2941 ggtgctcgca ccgctcctgg cggttatcac cgtccgatct tcatcgtcgg attgcagggg
     3001 gtatttcctg attaacaatg gtaggtgatt cgtcaacccc accgtaagac gcgacacgag
     3061 gtccctttca acagtaggaa ccggatgtgt tggctgtcaa gtgatccagc tagatcctgg
     3121 cgtagtgctg tacgcccgta ttcctgacca tgccctggag ctccagcatc atgagccgcc
     3181 cggagatctg ggagaccgtt gtgccacaca gcaatgccag gtcatcgata tgctgtggtt
     3241 cagaggtgat gaccgccgac agacggcgat cagcatcact catcggcagc gcctgctgcg
     3301 cagctgcgac ttcgcacttt tgcatgatct ggaggtcgtc gagaatgtcc tcggcgcttc
     3361 tcaccaagcg cgccccatca cgaatgaggc ggttgcagcc actgctattc gccgcattgg
     3421 ccggaccagg aacagcaaag acatcccgtc catgatcggc agcgaaatcc acggtaatca
     3481 aggctccttg cggtcgggcg cctcgacgac gaccacaccg agggacaggc cggagataat
     3541 cctgttgcgg ggcggaaagt tcacgccgtc cggaggtgta tcgggcaagt agtcggaaac
     3601 taccgctccc tgttcagcaa tgcgctgggc caggttgcgg tgttccggcg ggtagatccg
     3661 atccacgcca cttccgagta cggctatcgt gcgtccgcca gcatccaggg ctgcctggtg
     3721 cgctacccca tcgacacctc gggcaagtcc ggaaactacc gttaccccag cccgtgccag
     3781 gccagtagag attcgtgcgg tcatctcgcg tccgtaggcg gtaatacgcc aggtgccaac
     3841 aatggccaca gcgacacgat ccgtctcaag cagctctccc cggtagtaga gcacaggggg
     3901 aggagcggca acctggttca gcaacgctgg gtaactctcg tccactgtcg ttgccacacg
     3961 cgcccctgac cgttcgaggg aggccatcaa ctccggtaag tccagcccgt tccgggtttt
     4021 gaccagctcg ttgacgggtc gctcctcaag gcaacgcctc agctccgttg gccgggcatg
     4081 ccaggcacgc tccaggctgc cgaaggtctc gagaaggcgc cgcagcctgg cggtcccgat
     4141 atacggcacg cgatgaaacg ccacccagaa cgctcgctcg gggcgtcctg cgctccactc
     4201 aatgccacgt tctgctgagt ccgtagtcgg gatgacctcg tcaattccca tttgcgtccc
     4261 agcgatcctt ctatagaatg gcgcgagttc ggacggtgga gattcacggt cgcttaccgg
     4321 cgaccaattg agagagtgag agaacatgaa cccaacaaac cgggttaagg aaaaactgtc
     4381 agctggcgag cgtgtattcg ggtgctttat accacttgct tcgcccgaag tggtcgaagt
     4441 ctgcgccctc accgggttcg atttcgcatt gatcgacgcg gagcacgggc ccatctcgcc
     4501 ggagacggcg tacccgatgg tcctggccgc tgagtcccgg ggaatcgagg cgttcgcccg
     4561 cgtcggccag gccgacaagc aaatggtgct gaaatttctc gacattggtg tctccggcat
     4621 tatggcgccg caggtgaaca ccatcgaaca ggcaactgcg gctgtggccg gcaccaaata
     4681 ttttccaacg ggcaataggg gcctcgctgg cggccgaatg ttcgactttg gactcacggc
     4741 gcacctgtcg gccctggtgg agccgctcaa tgaccgtgtc ctcacgatga tccagttcga
     4801 gcacgttgac gcgttgaagg accttgatgc aatcctcgcc ctggagcatc tcgatgtcct
     4861 gttcgtgggt ccaaacgatc tcgcgcaatc gctcggctat ccgggccagc ccggccaccc
     4921 ggaggtcacc cgagttgccg atgaggtcgt cgctcgcgcc aagggtgccg gcgtaaaaac
     4981 aggaacggtt gccttcacag tcgaccaggt tcacaacgtg ttcgggaggg gttttgacat
     5041 ggcagtcgcg aatgctgttg ggctcctcgc ggggggcgcg aaagcataca tcgagggcgg
     5101 ccgccaggcg ccatagtgag gcatacgagc ataaaatagg gaagcggcgg tctccctgta
     5161 agaaccgccg cttccctgct cgcgccagaa cgtcgtgatc gtaggaagtt gatcaggtca
     5221 tcttgcgtag agcgaagaga atgatcactg cgccgattac ggcaacgata atggagccaa
     5281 tgatgccccc gcccacgccg agatcaagca ggctattgaa gatccaaccg ccgagtatgg
     5341 ctccgacgat gccgacgacg atcgtgccga tcaagccccc cggggttcgt ccggggacga
     5401 gatagccagc gatcgcacca gcgatgagtc caataatgag gtaataaata atttccatgc
     5461 tgttccctct cctgccttcg acagcgaagc gtatcccttg atgaagggtc ccccctggac
     5521 ccactcgtca tcacggttcc cgcgcaaact atgtgccaag ctgagtgaag tctggtgaaa
     5581 gggcaggacc agcgagaggc cgaaggaaaa ttgtcggcga ttgcctccaa ttcaattgtc
     5641 tgcacaaggg cgctgtgtta tcgtgcacat gtagtatcga gcgagtacag gaacaatccc
     5701 ctatgggcgc aacgaagcgc ccggccagtg gcggcaatca ttgcgatagc tgtcgttgtt
     5761 cgtgtatgaa atacgttgta cagcggatgt gtcgtgaggc tgtcacgggc cgcgctgtac
     5821 gcacagcagg ttccgtttta ggggagagag gagcaaggat ggcggctgag ccacagacca
     5881 ccacttctgc tgcagtaaga cagcgttctg gagagccgct ccttgaggtt aagaacctca
     5941 agacccagtt cttcacgcag gatggcgtcg tcagggcggt cgacgacgtt tccttctata
     6001 ttatgcccgg cgagacgctg ggcgtggtgg gtgaatccgg ctcgggcaaa agcatgacgg
     6061 gtctctccct catgcgcctg atcccgaacc cgccaggcaa gatcgtgaat ggcgaggtta
     6121 tcttccatgg acgtgatgtc ctcacgatgt ccgaggacga ggtccgggga atccgtggga
     6181 acgacatcgc gatgatcttc caggacccga tgacttccct caacccggtg ctcacgatca
     6241 accgccagat tagtgaatcc ctcattctcc atatgggaat gaacaagaac caggcgaagc
     6301 agcgcgcgat cgagctcctg gacatggtag ggattcccaa cgcggacgag cgcgtggacc
     6361 agtatccaca ccagttctcc ggtggtatgc gccagcgcgt catgatcgcc atggcgctct
     6421 cgtgtaaccc gcagctgctc atcgccgacg agcccacaac cgcgctcgac gtgaccattc
     6481 aggcccagat cctggatctg atgactacgc tccagagcga tacgggaacc ggcgtcctgt
     6541 tgatcacaca ctcgatgggt gttgtggccg gtatggcgga t
//
LOCUS       DASYWM010000254         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_367772, whole genome shotgun sequence.
ACCESSION   DASYWM010000254 DASYWM010000000
VERSION     DASYWM010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367772"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VGR29_10400"
     CDS             <1..205
                     /locus_tag="VGR29_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074038.1"
                     /translation="VAQPTSPPLLRSSIHPNRGLSLLRFATRSIRRAGRATVIGSASF
                     ATELQPLGETFQVFKYITWPVAA"
     gene            complement(826..1485)
                     /locus_tag="VGR29_10405"
     CDS             complement(826..1485)
                     /locus_tag="VGR29_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV2074039.1"
                     /translation="MYIIVVGGGKVGYYLTKTLLADGHEVLLIEQDDRKVETYSERLG
                     ACVLEGNGAEAAVQSNAGAARADVVVAVTGEDEDNLVICQMAKQKFQVGRTIARVNNP
                     KNEELFRLLGIDVTVSQTNHILNLIGQAIPERSLIHLLNLRHESLAIIEATISERSPV
                     ANMAIDDIELPLDCAIAAIERGNDLIVPSSSTVIKPGDEIIAVTHRAQEDELRRLLVV
                     E"
     gene            complement(1489..1923)
                     /locus_tag="VGR29_10410"
     CDS             complement(1489..1923)
                     /locus_tag="VGR29_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV2074040.1"
                     /translation="MNVVIMGSGRVGARVASMLDVNGHRVTVIDLQSDAFRKLPDDFG
                     GDTVIGTGIDEDVLRVANIEEADVFIAVSENDNRNIMAAQVARKIFDVPQVILRIYDP
                     VREDTYRRMGYSTVCPTTTISAMILDQVITSGESRRSAAREA"
     gene            complement(1920..2444)
                     /locus_tag="VGR29_10415"
     CDS             complement(1920..2444)
                     /locus_tag="VGR29_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2074041.1"
                     /translation="MVSLRQAFRGSTETTELPVWSRIIVPVTDIDIDLRVLQMVCRIA
                     LRQRIEVTLVYVVEVVQSMPLDAELPSEVARGEHALQASFDFIHRQVPDKRSTVNTEL
                     LQARSAGAAIVDEAIERDAGVVMLSARLQRKRGQLTVGETVDYVLRHAPCEVIVVRHE
                     MPDWLVHALELDQE"
     gene            complement(2522..>3384)
                     /locus_tag="VGR29_10420"
     CDS             complement(2522..>3384)
                     /locus_tag="VGR29_10420"
                     /inference="COORDINATES: protein motif:HMM:NF045129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2074042.1"
                     /translation="LYMAGQVNGTSGYEEAGGQGIVAGANAAARIRDMEPLILGRADA
                     YIGVMIDDLVSTVLDEPYRMLTSRAEYRLILRSDTADARLAALAYERELIGTDRAEAI
                     ADEKAGSQALVSSLEKIWLGNNDRHAAALDAEGLAPATRSMSALELARRPGAPLDAVI
                     RALGRLGFWTDQVPTGLLLDRTEVAIKYGSFIDKEEREASRQLRSGGEQLPPDLDYSL
                     VPGLRVEAIQRLQIASPLTVGQAARTAGVTPSDIGALLIHLTRDRNRTTRDPDHHCHR
                     DPVTPGYRVR"
BASE COUNT          665 a         1021 c         1005 g          693 t
ORIGIN      
        1 agtcgcgcag ccgacatccc cgcccctttt gcggtcgtcg atccatccga accggggact
       61 ttccctattg cggtttgcaa cgagatcgat acgccgcgcc ggccgggcga cggtaatagg
      121 cagcgcctcc ttcgctacag agttgcaacc tctcggagaa acgtttcagg tgttcaagta
      181 cataacctgg ccggtggcag cctaaaggtt gctgcagcct gatgcccttg acgtgagggg
      241 attttcttta gacggccaag gcgtttgcag ggtgggcgat aacctggcca tgtacgacgc
      301 gattggagga cgaaggctgc aatgtgatta atgtggcagt gtggcagcct tggcccttgg
      361 gagagtcact cgccccaatc cacggggttt tcaggtctgc cacattgttt catggtatgt
      421 ggcagccttg atgaggtcgg gcaaatgtcc ccagcgagac ggtacagacg ctctctcggt
      481 aatacgcatc caggaagagg atctgcgccg ttatctcacc cggctcaggt taaagcggtt
      541 tgcgtgatga ttgactgccc gcccaggaag cggagcatcc cacgcttcgc tccggacagg
      601 ctcttgtgtg ctcccttgtt ctggaacggg aggacatgga gtcctcccct acgggagggg
      661 ggagccctat ctttcctgca gcccgctcca accaacgccg aacgcggctg ccgtgtttct
      721 tgcgctcggt actgcctcgt cgctgaatgc gcccccaggt attccaggcg atcaatcagg
      781 atggtcgatg ttagcaggag acttcggcac atcgctggac ggggattact cgacgacaag
      841 caaccgccgc agctcgtctt cctgcgctcg atgggtcacg gcgatgatct catcgccggg
      901 cttgatcacc gtactggaac ttgggacgat gaggtcgttt ccccgttcaa tggcagcgat
      961 ggcacaatcg agaggcagtt caatgtcatc gatggccata ttggccaccg gcgaacgctc
     1021 cgaaatggtt gcttcgataa tggccaggct ctcgtgccgg agattcagca gatggataag
     1081 cgagcgctcc ggaatagcct gacctatgag attgaggatg tggttcgttt ggctgacggt
     1141 gacatcgata ccgagcaggc ggaacagttc ctcattcttc gggttgttga ctctcgctat
     1201 cgttcgcccc acctggaatt tctgcttcgc catctggcag ataacgaggt tgtcctcgtc
     1261 ttccccggtg acagcaacaa ccacatcagc gcgagccgcc ccagcattcg actggaccgc
     1321 ggcttcggcg ccgtttccct caaggacaca ggcgccgagt cgttccgaat aggtttctac
     1381 cttccggtcg tcctgctcga tcaggagaac ttcgtggcca tcggccagga gcgtcttggt
     1441 caggtaatat ccgaccttac caccgcccac aacaatgatg tacacggcct aggcctccct
     1501 tgccgcggag cgacggcttt cgccgctggt gatcacctga tcaaggatca tggcggagat
     1561 ggtggttgtg gggcacaccg tggagtagcc cattcgccga tatgtgtcct cgcgcacagg
     1621 atcgtagatg cggaggatca cctgcggcac atcgaagatc ttgcgggcca cctgagccgc
     1681 catgatgttc cggttatcgt tctcactcac cgcgataaag acatcggcct cttcaatgtt
     1741 ggctacccga aggacatcct catcgatgcc agtgccgatg acggtatcgc cgccgaaatc
     1801 gtccgggagc ttccggaagg cgtccgactg caggtcgatc acggtgaccc tgtgcccgtt
     1861 gacatccagc atggaggcga ctcgtgcacc gacgcgtcca gagcccatga taacgacgtt
     1921 cattcctgat ccagttccag cgcgtgaacg agccaatccg gcatctcgtg ccgcacgacg
     1981 ataacctcgc agggagcgtg tcgcagtacg tagtccacgg tttcgccgac ggttaactga
     2041 ccgcgcttgc gttgcagcct cgcgctcaac atgaccacac cggcatcccg ctcgattgct
     2101 tcgtcgacaa tggccgcccc ggcggatctg gcctgcagca gctcagtgtt gaccgtgctg
     2161 cgtttatcgg gcacctggcg atggataaag tcaaaggatg cctgcaaggc gtgttcgcca
     2221 cgcgctacct cagagggcag ctcggcgtcc agtggcattg actggacaac ctccacgacg
     2281 tacacaagtg tgacttcaat gcgctgccgc agggcgatac gacaaaccat ctggaggacg
     2341 cgtaagtcga tatcgatgtc ggtaaccggc acaatgatgc gggaccagac cggcagctcg
     2401 gttgtttcag tacttccccg aaacgcctgg cgcaaactga ccaaagagca accaccttat
     2461 cgtgaacgtg tcgagtggag ggtccaaaac accctccatg gcgaagatgt aacgcgggag
     2521 tctaccgcac ccggtaaccg ggagtcacgg ggtcgcgatg gcaatggtga tctgggtctc
     2581 tggtcgttcg attacggtca cgtgtgaggt ggatcagcag tgctccgatg tcgctcggag
     2641 tcactcccgc cgtgcgtgcg gcttggccca cggtgagggg actggcgatc tgcagtctct
     2701 ggatcgcctc aacacgcaag cccggaacca gagagtaatc gagatctggt ggcagctgct
     2761 ccccgcccga cctcagctgg cgggatgcct cgcgctcctc cttgtcgatg aacgatccgt
     2821 atttgatcgc gacctcggtc cgatcgagga gcaagccggt agggacctga tcggtccaaa
     2881 atccgagcct ccccagcgca cggatgacgg catcgagggg agcaccggga cgacgggcaa
     2941 gctcgagtgc cgacatcgac cgggtggcgg gcgccaagcc ttccgcatca agtgctgccg
     3001 catggcggtc gttattgccg agccagatct tctcgagcga tgagacaagc gcctgacttc
     3061 ccgctttctc gtcggcgatt gcctcggcac ggtcggtacc gatgagctcc cgttcataag
     3121 ccaaagcagc cagacgcgca tcggctgtgt ccgacctcaa tatcaggcgg tactcggcgc
     3181 gggaggtgag catgcgatag ggctcgtcga gaaccgtgga aacaagatcg tcaatcatca
     3241 cgccaatgta ggcgtcggct cgacccagga tgagcggttc catatcccgg atacgcgccg
     3301 ccgcgttcgc ccccgcgaca atcccctggc cgccggcctc ttcgtaaccg gacgttccgt
     3361 tgacctgacc ggccatatat agtc
//
LOCUS       DASYWM010000255         5874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_367797, whole genome shotgun sequence.
ACCESSION   DASYWM010000255 DASYWM010000000
VERSION     DASYWM010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367797"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..598
                     /locus_tag="VGR29_10425"
     CDS             80..598
                     /locus_tag="VGR29_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074043.1"
                     /translation="MQLPYRVRQFIVHVSADRQGPPHLPVDALRRLTEPMEIQFGILP
                     PGDQRHLLHVFRRLTLWDTDEDTATAGLLHDVGKACRTCNIGIADRCAHVVLNRLVPG
                     PYSLFARLDAPPRWLMGLHRLANHSHRGALAARQAGYSERVCWLIEHHERGGDTDDPE
                     LQLLRKADDSSP"
     gene            595..1425
                     /locus_tag="VGR29_10430"
     CDS             595..1425
                     /locus_tag="VGR29_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEV2074044.1"
                     /translation="MSTQQPTLVPTLSDYQTRLPSFEGPMDVLLRLIERNRLEIADVS
                     LIQLTDQFLDFVVAMTAAPPEVIAEFAMVGTRLTLLKSRSLLPRPPVVEAADEPDPSD
                     LVRQLQAYKQLKVAAHSLGERRESGLVSFRPQAAGAIAGPGQVAPPRLAQYESSVLIR
                     SIRRRLSAVPQTMQTIRQRRIVSIREMIERVTQAASSLPSFRFSDVTCGLQSRSEVAT
                     AFLAVLVLIRGRSLEASQQGLFADIRLAPTSAVDEIEGDPRDVETGFGLKSPILEHPK
                     "
     gene            1422..2126
                     /gene="scpB"
                     /locus_tag="VGR29_10435"
     CDS             1422..2126
                     /gene="scpB"
                     /locus_tag="VGR29_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEV2074045.1"
                     /translation="MSEPKIMPGTGKSGPANGQLRLPTRSPSTLEEGELGALIEALLL
                     VAQEPPAIEDLASAAEVSSEEINRALSLMEQRDDRGWVIQRHGQRVHIATAPRFSEQV
                     RLFLGLDREAKLSSASLEALAIVAYQQPVTRAEIEQVRGVDSSGVLAKLHARGLIEPV
                     ARLTTVGNPIQYGTTADFLSHFGLRSLNELPPLGEIGGRDGKELLAHAAATTAQDAEP
                     EDEDPHAHPASSDDLR"
     gene            complement(2430..3623)
                     /locus_tag="VGR29_10440"
     CDS             complement(2430..3623)
                     /locus_tag="VGR29_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEV2074046.1"
                     /translation="MSYGTDAIYLDHAATTPTDPTVVGSMLPFFGQQFGNPSSIYRAG
                     QDARAALDRARAQVARVLECRLSEVIFTSGATESIGHALRGVAWAARLKAPGGPAPHV
                     ITTTVEHHAVLHTADALERQGFAVTRIGCDREGRVRAADIEAAIRPETCLISVMYANN
                     EIGTIEPVADIGALAHERGVLLHTDAVQAAGVLPLNVDDLHVDLLSLSAHKFYGPKGV
                     GVLYARKHTPLEWQQQGGGQESGRRGGTENVPLIVGMAEALTRAEAVRDQYNIFCAKL
                     RDDLWAQIQERISGVSLNGPQPGTDRLPNNLNISIEGVQGETVLLSLDMGGVAASAGS
                     ACTTGNSEPSHVLLACGLTEQEARASIRLTVGRGNSEAEIGEAVDVLEDAVARVRTLA
                     SAGVV"
     gene            complement(3678..4136)
                     /locus_tag="VGR29_10445"
     CDS             complement(3678..4136)
                     /locus_tag="VGR29_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV2074047.1"
                     /translation="MKVSTRGEYGVRAMVALAHGFGNGPMSITEIARQSNVPPAYLEQ
                     LIAPLRRASLVESKRGARGGYVLAREPESIRIGDIYRVMEGPVAPMDCVSEDLSEQTC
                     PLIDGCETRPVWLKVRDSIIDALDSTTLADLTQTPQQQSVSESANCTTPV"
     gene            complement(4213..5379)
                     /locus_tag="VGR29_10450"
     CDS             complement(4213..5379)
                     /locus_tag="VGR29_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2074048.1"
                     /translation="MIQAFRVGRVLGVDVNIHPTFALILLFAFFQWGIGEDGGVLPFF
                     LGCLLVILVFLSVLAHELGHCLMARQFGIEVLDITLWPFSGVARIEQMPASPRHEFLI
                     ALAGPGVNLGIFMLLLPPVLLVGVLAGGDTFFSPGGLIGALEPASMLAAVAILNLGLM
                     VFNLLPAFPLDGGRMLRASITSAFGRTRATSIATRLGIGIAIVMIVIGTWQRDILFPM
                     LGVFIIFAAQAETRMVRVEDQMRRLKVGQFSLWDMGGISPSDPLTFALRGGPRDLVVT
                     DNGRVVGMLWRSQLLDGLQGGVAGRTVADVMDRAIYVADVEDSVFDVQQHMNRTQRWA
                     IPVTEEGAYRGIFTAERFVSLYRQIAPGLRERHWSLSDEWKEAISVNLRRRRNG"
BASE COUNT         1218 a         1856 c         1676 g         1124 t
ORIGIN      
        1 ttggcggaat gatcgatccg gtccgggaca ccctctacaa tgtcgtgttc actggcctta
       61 tttaacgaga ccggtatcca tgcaactccc gtatcgtgtt cgtcagttca tagtccacgt
      121 cagcgccgat cgccagggtc ctccccacct tccggtcgac gccctccggc ggttgacgga
      181 gccgatggag atccagttcg ggattcttcc gccgggagac cagagacacc tcctgcacgt
      241 gttccgccgc ctgactctgt gggacaccga cgaggatact gcgaccgctg gactgctaca
      301 cgacgttggc aaagcgtgcc ggacgtgcaa catcggcatt gcagaccgct gcgctcatgt
      361 tgtccttaat cgactggtcc ccgggcccta cagcctgttc gcaagattgg acgcacctcc
      421 gcgttggctc atggggctcc accgcctggc aaatcactcc caccgtggag cattggcagc
      481 acgccaggct ggctatagcg aacgcgtctg ctggctcatt gagcatcacg agcggggcgg
      541 tgacaccgac gacccggaac tccagttgct tcgcaaggct gacgattcat cgccatgagc
      601 acccagcagc caacgttagt ccccacgctc tccgactacc aaaccaggct tccttcgttt
      661 gagggcccca tggatgtctt gttgcgactt atcgagcgca atcggctcga aattgccgat
      721 gtttcactca tacagctaac cgaccagttc ctcgacttcg tggtcgcgat gaccgcggca
      781 cctcctgaag tcatcgccga gttcgccatg gtcggaacaa gactgacgtt actgaagagc
      841 cgttcgttgc tacctcgtcc tcctgtggtt gaggcggcgg atgagccgga tccaagcgat
      901 cttgtccgcc agctccaggc atacaaacag ctcaaggtgg ctgcccacag ccttggggaa
      961 cgccgcgagt ccggccttgt ctcctttagg ccccaagccg ccggagccat agcaggtccg
     1021 ggtcaggtcg ccccgccccg gctcgcgcag tacgaatctt cagtgcttat ccgttcgatt
     1081 cgccgacgct tgtcagccgt tcctcagacg atgcaaacta tccgccagcg gcgtatcgtc
     1141 agcattcgcg agatgatcga acgggtcacc caggccgctt cctccttgcc gtcattcagg
     1201 ttcagtgacg tgacctgcgg gttgcagtcc cggtcggagg tggccacagc attcctcgct
     1261 gttctggtcc tgattcgggg aaggtcgctc gaggcgtcgc aacagggcct gttcgcggat
     1321 attagacttg ctcccacttc cgctgtcgac gagattgaag gagacccgcg agacgtcgaa
     1381 accggtttcg gcctgaaaag cccgatcctg gagcatccca aatgagtgaa ccgaagatta
     1441 tgccaggcac tggaaaatcc gggcctgcga atgggcagct acgactcccc acccgttctc
     1501 cgtctaccct tgaggagggc gaactgggcg cgctgatcga agcgcttctt ctggtggcgc
     1561 aggagccgcc ggcgatcgaa gatttggcgt cagcagcgga agtatcatca gaagagatta
     1621 atcgcgccct gtccttgatg gaacagcgcg acgatcgggg ctgggtgatt cagcggcacg
     1681 ggcagcgagt ccatatcgcg acggcaccgc gtttctccga gcaggtgcga ctctttcttg
     1741 gcctggaccg cgaagccaaa ctctcttccg cgtccctcga agcgctggcg atcgtcgcct
     1801 atcagcaacc ggtgacgcgc gccgagatcg agcaggtgcg aggcgttgac tcctcgggcg
     1861 ttctcgcaaa gctccatgcc cgtggactta tcgagcctgt tgcccggctc accacggttg
     1921 gaaatccaat tcagtacgga accaccgccg atttcctcag ccactttggg cttcgatcac
     1981 tgaacgaact cccacccctc ggcgaaatcg gcgggaggga tggaaaagag ctgctcgcac
     2041 atgccgccgc gaccaccgcg caagacgcgg aaccggaaga cgaggatccg catgcccatc
     2101 ccgcatcttc agacgatctc cggtgatcga tcggctaacg cccccgattc agattcaact
     2161 gcccaagggt gtcatttcca ccagaggcgc cacctaaagg ctcgtggaat acatgctgct
     2221 gcatgcgtgg cctgcctcag gttcatagtg gatcgagggc tctcaatgtc ttcccagcgg
     2281 ctcggagccg accgctacgg gctctggcaa tggagggtgg cgcttcaggt tctcccggaa
     2341 agctggaggt tgaaggacac cgccgacacg ggttgtcttc atgctcttgg aaccacgcgt
     2401 ggtccgagca aggcattcgc ggtcgggagt tagacgacgc ctgcactcgc caacgtccgg
     2461 acgcgcgcga ccgcatcttc gagtacgtcg accgcttcac cgatctccgc ctctgagttt
     2521 cctctcccga ctgtcaaccg gatactggcc cgagcttcct gttcggtaag cccgcaggca
     2581 aggagtacat gacttggctc ggagttgccg gtcgtgcagg cgcttccagc cgacgccgcc
     2641 acgccaccca tatccagact caacagaacc gtttccccct gcaccccctc tatcgatatg
     2701 ttgaggttat tcggaaggcg gtctgtgccc ggctgggggc cattgagaga gacgccgcta
     2761 atccgttcct ggatttgggc ccagaggtca tcgcggagtt ttgcgcagaa tatgttgtac
     2821 tgatccctca cagcctctgc ccgcgtaagc gcttcggcca ttcccacaat cagcggcacg
     2881 ttctcggtac cgccacggcg tcccgactcc tggccgcccc cctgctgctg ccactccagc
     2941 ggcgtgtgct ttctggcgta gagcacccca acacccttgg ggccatagaa cttgtgagcg
     3001 gaaagcgaga gcagatcgac gtgcaaatca tcgacattca ggggtaggac gccggccgcc
     3061 tgaaccgcgt ccgtatggag caggacgcct cgctcatgcg ccagggcgcc tatatccgcg
     3121 acgggctcga tcgtaccaat ctcgttgttg gcgtacatga cagaaatcag gcatgtctcg
     3181 ggacggatag ccgcctcgat gtctgcggcc cgcacccgcc cctcccgatc acatccgatg
     3241 cgagtgacgg cgaatccctg tcgctcaagg gcgtccgcag tatgcaagac cgcatgatgc
     3301 tctaccgtcg ttgtgatgac atgaggcgcc ggtccaccgg gggcttttag gcgggccgcc
     3361 caggcgactc cccgcaacgc atggccgatg ctctccgtcg cgccactcgt aaagatcacc
     3421 tcagagagcc tgcattcaag cacgcgtgca acctgcgcac gggcacgatc aagtgccgct
     3481 cgggcatctt gtcctgccct gtagatactt gacgggttgc caaattgttg gccgaagaac
     3541 ggcagcatgg atccgaccac tgtggggtct gtaggagtgg tcgcggcgtg gtcgagataa
     3601 atcgcatcgg ttccatacga catcagacca gttccttcat ttgcgtgatc tcgggatccg
     3661 taggagtgga gcgtgggcta gaccggggtg gtgcagtttg ctgactcgct aaccgattgc
     3721 tgctggggag tctgcgtcag atcggccagt gtagtcgaat caagcgcatc gatgatggaa
     3781 tcgcgaacct tcagccatac agggcgggtc tcgcaaccgt caatcagcgg gcacgtctgc
     3841 tcggaaagat cttccgacac gcagtccatg ggtgcgacag ggccttccat cacgcggtag
     3901 atgtcaccga tccggatcga ttctggttca cgcgcaagca cgtaaccacc gcgggctccg
     3961 cgcttgctct ccaccagcga tgcccgtctc agtggagcaa tcagctgttc cagataggct
     4021 ggcggcacgt tcgactgccg ggcgatctcg gtaatcgaca ttgggccatt accaaacccg
     4081 tgtgcaagcg caaccatcgc gcgcacacca tattcgcccc gtgtcgatac cttcatacag
     4141 ttgatcctcc gcagggatat accatccctg acagtactct accccttccc aagcaattgt
     4201 caagccagca ggttagccgt tccgtcgccg gcggaggttc acagagatcg cctccttcca
     4261 ctcatccgag agtgaccagt ggcgctcccg caagccagga gcgatctgtc ggtaaagact
     4321 cacgaagcgc tcagcggtga aaatgccacg gtaggctccc tcctcggtca cgggaatagc
     4381 ccaccgctgg gtccggttca tatgctgttg cacatcgaaa accgagtcct cgacatccgc
     4441 gacgtagata gcccggtcca tgacgtctgc cacggttcgc ccggctacgc ctccctgcaa
     4501 gccatcaagg agctgcgagc gccacagcat tcccaccacc cggccgttgt ctgttactac
     4561 caaatcgcgg ggaccgccgc gcagggcaaa ggtaagcgga tcggagggtg agattcctcc
     4621 catatcccag agggagaact ggccgacctt gagccggcgc atttgatcct cgacgcggac
     4681 catccgggtc tccgcctgtg ccgcgaagat aatgaagacg ccgagcatgg ggaacagaat
     4741 gtctcgctgc catgtgccaa ttacaatcat gacgatcgcg atgccgatcc cgagacgggt
     4801 cgcgatagac gtcgccctgg tgcgcccaaa cgctgacgtt atggacgcgc gaagcatcct
     4861 tccgccatct agcggaaacg cgggcagcaa attgaagacc atgagaccga gattgaggat
     4921 tgccaccgca gccagcatgg aggccggctc cagtgctccg atcagtccac ccggggagaa
     4981 gaacgtgtcc ccccccgcga gaacgccaac gagcaggact ggaggcaaca gcagcatgaa
     5041 gatgcccagg ttcactcccg gtcccgcgag cgcgatcagg aactcatgtc ggggagacgc
     5101 gggcatctgc tcgatgcgag caacgccgct gaacggccaa agcgtgatgt ccaggacctc
     5161 gatcccgaac tgacgagcca tcaaacagtg acccaactcg tgcgcaagga cagagagaaa
     5221 cacgagtatc acgagcaggc aaccaaggaa aaagggcaga acgccgccgt cttcacctat
     5281 cccccactgg aaaaaggcaa agagaagaat cagggcaaat gtagggtgga tgttgacgtc
     5341 gacgcctaag acgcgtccga ctcggaaggc ctgaatcacc cgctcaatcc ccaaacacac
     5401 accgacgact tctccaacag caaacagggc caatctgaac ttgtcggtac ctcgggatcc
     5461 gaaagaacca caggcaacag gacgtcgccc gcaacaattc tacatgggga cactaatctt
     5521 cagtgacaga ggccgctgga cagcgttttc ttcgcaagaa cgttgccacc agtcacaaca
     5581 ccccatatac tgatgacgca atgcatcggc gctacgagct ctcgtccctt catctctcca
     5641 tgcatcgctt gcagcagtaa tagtggattt gtgcgcctca acgtgaagga gtacctttga
     5701 atggcatgga tggaaaccgg gtttgagttt ggccaggaga tgcggccctg ggtttctccg
     5761 agcctcattg aagcgaatta catcctgagc ctctcgcagc aggagctgca gaacgtcatc
     5821 gaggccgaga tggcgcagaa cccagcgctc gagattgagg acaggtcgac gtgc
//
LOCUS       DASYWM010000256         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_536950, whole genome shotgun sequence.
ACCESSION   DASYWM010000256 DASYWM010000000
VERSION     DASYWM010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536950"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1136
                     /locus_tag="VGR29_10455"
     CDS             162..1136
                     /locus_tag="VGR29_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV2074049.1"
                     /translation="MRPIIKDYARMIAVAIFFLGVFGLASMLLQRTNADNEVEPVQAT
                     PVADASKDLLVDVDWLAQRLDDGTAAPIIIDVGEADQYAREYIPGAVHLWWQDTMNLN
                     GAGYGEAFGLASDASYRPEIGASQDQSIVVYDNSGSKWASRLVWQLRTSGYSNAVVLD
                     GGLSAWKGAGNPTTSSPATPSAVASPVDTWVQDNEITTDQLADWLDDPNVVVIDTRSD
                     DQKQDTVNDTIRTGQIPGSLSLPASAVIRDDGTFASPSELREILAPLGLEQENTIVVY
                     GRFGVETGQVWLALRLAGYEDVRVYDDGWITWGYDEDLPIEPIGTPMS"
     gene            complement(1315..>3019)
                     /locus_tag="VGR29_10460"
     CDS             complement(1315..>3019)
                     /locus_tag="VGR29_10460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEV2074050.1"
                     /translation="TASFSRLVEEITSRESSSDVTADADSVLDALIRLLDRVSIIMPY
                     VHLHLAVNSRDKLAQRLMSECQIALLPFRQANTRLTAWLGKQNIEEYIASSDIAREHA
                     YLLRKTKVEAKHLMPQAEEGLASEMTLSGGSAFGKLHDDLTSQIMVRFEKRPGEEVEL
                     PMSEVRNLATDPDRDVRRRAFEAEITAWKQWETPIAAALNGVKGEHTTLARRRNWESG
                     LEQSLFQNHIDRETLDAMMQAAREAFPDIRRYYDAKARALGIDTLMWYDIIAPVGNNN
                     REWSWDEGMSFLMEQFGTYSHRMRDMVQQALDEQWIDAEPRPGKVGGAFCSGTRGGKS
                     AVLSNFTPSFEGVSTMAHELGHAYHNLCQKPRTVLQRSETPMTLAETASTFCETILRK
                     AAIANGSTDEKFPILEGALVDAAQIVVDISSRFLFEQAIFEQRAERQLTADEFGVIMT
                     DAQKQTYGDAIAEEGLHPYMWAVKGHYYSPNFAFYNYPYMFGLLFGLGLYARYQEDPE
                     TFRAGYDDLLSSTGMADAADLAGRFGFDIRTPDFWRSSLDVIRHDVDEFVALVEQKYG
                     A"
BASE COUNT          584 a          925 c          887 g          623 t
ORIGIN      
        1 acgtagaggt ggtctacatc atcgagcagg gcaatggata ggtcctttca ctccctctag
       61 acgccacttc tttcggctcc tggcgaatgc ccggagccgt tcgcgtatca tgtccccgaa
      121 ccggacgcca tagtcaccgg ccggccgagg agtttgcccg catgcgaccc atcatcaagg
      181 actacgcgcg aatgatcgct gttgcgatct tcttcctcgg ggtcttcggc ctcgcatcga
      241 tgctgctgca gcgcaccaat gccgacaacg aggttgagcc ggtccaggca actcccgtcg
      301 cggatgcaag caaggatctc ctggtggacg tcgactggct cgcccaacgg ctggacgacg
      361 gtaccgccgc ccctatcatc atcgatgtcg gggaagcgga tcagtacgca cgtgagtaca
      421 ttcctggcgc cgtccatctc tggtggcaag acacgatgaa ccttaacggc gctggctacg
      481 gtgaggcttt cggactcgcc agcgacgcgt cgtatcgccc cgagatcggt gcatcccagg
      541 accaaagcat cgtcgtctac gacaacagcg ggagcaaatg ggcctcccgg ctggtttggc
      601 aactcaggac gagcggctat tcaaatgctg ttgtccttga cggaggtctg tccgcgtgga
      661 agggagctgg gaatcccaca acttcttctc ctgccacgcc gtcagccgtc gcatccccgg
      721 tcgacacctg ggttcaggat aacgagatca ccacggacca gcttgccgac tggctggacg
      781 accctaatgt cgtggtgatc gacacccgga gcgacgatca aaagcaggat accgtcaatg
      841 acacgatccg gaccggccag attcccggtt cgctctccct tccggcctct gctgtcattc
      901 gcgacgacgg cacctttgcc tcgccgtccg agcttcggga gatcctcgcg ccgttgggtc
      961 tggagcagga aaataccatc gtggtttatg gccgcttcgg ggtcgaaacc ggccaggtgt
     1021 ggctggcgct tcgtctcgcc gggtatgagg acgtgagggt ctacgacgac ggatggatca
     1081 cctggggtta tgacgaggat ttgccaatcg agccaatcgg tacgccaatg tcgtgatgcg
     1141 gagcgctggt acgggagtat ggtcgattgt ggatgctggc cggatcgaaa agcgttaagc
     1201 agttgccgcg agacacaagg tgcccgcgag catgcgccga tgtacttgcg acacaaataa
     1261 gaagcccgga gctctttcga gattccgggc ttcagtgtct ttggccagtt caggctacgc
     1321 tccgtacttc tgctcgacca gggcaacgaa ctcatcaacg tcgtggcgga taacatcgag
     1381 gctcgaacgc cagaaatccg gcgtccggat atcaaacccg aaccgccctg cgaggtcagc
     1441 ggcgtccgcc atgccggtcg acgagagaag gtcatcgtag cccgcgcgga aggtttccgg
     1501 atcctcctgg tacctcgcat agaggccgag gccaaaaagc aagccgaaca tatacgggta
     1561 gttgtagaac gcgaaattgg ggctgtagta gtgccccttg accgcccaca tataggggtg
     1621 gagcccttcc tcggcgatcg catcgccgta ggtttgcttc tgggcatcgg tcattattac
     1681 gccgaattcg tctgccgtga gctgtcgctc agcccgctgc tcgaagatgg cctgctcaaa
     1741 gaggaaacgg ctgctgatat ccaccacaat ctgggcggcg tccaccagcg caccctccag
     1801 aatcgggaat ttctcgtcag tactgccatt cgcgatcgct gccttccgca ggattgtttc
     1861 gcagaaggtg ctcgctgttt ccgcgagtgt catcggtgtc tcggagcgct gcaggactgt
     1921 ccggggcttc tggcagagat tgtggtaggc gtggccgagc tcgtgggcca tcgtgctgac
     1981 accctcaaac gatggcgtga agttcgagag cacagccgat ttgccgcccc ttgttccaga
     2041 gcaaaacgcg ccacccacct taccggggcg tggctcagca tcgatccact gctcgtccag
     2101 cgcttgctgc accatatccc gcatcctgtg ggaataggtg ccaaattgct ccatgaggaa
     2161 gctcatgccc tcatcccacg accactcgcg attgttgttg cccaccggtg caatgatgtc
     2221 gtaccacatc agcgtatcga tcccgagagc cctcgccttg gcatcgtagt accgacgaat
     2281 atcggggaac gcctcccggg cggcctgcat catcgcatcc agcgtctcgc ggtcgatgtg
     2341 attctggaag agcgattgct cgagcccact ctcccagttc cggcgccgcg ccagggtggt
     2401 gtgctcaccc ttgacgccat tgagtgccgc agctatcgga gtttcccact gcttccaggc
     2461 ggtgatctcc gcctcgaatg cccgccgccg gacatcgcga tcggggtccg tggccaggtt
     2521 tcgcacctca ctcatcggca actcgacctc ttcgcccggt cgcttctcaa agcggaccat
     2581 gatctgggag gtcaggtcgt cgtgcagctt tccgaaggcc gagccaccgg agagcgtcat
     2641 ctcgctggcg agtccctcct ctgcctgcgg catcaggtgc ttggcctcaa ccttcgtctt
     2701 ccgcaaaagg tacgcatgtt ccctggcaat atcagacgat gcgatatatt cctcgatatt
     2761 ttgcttgccc aaccaggcag tcaagcgggt atttgcctga cggaagggca gcagggcgat
     2821 ctggcactcg ctcatcagtc gctgcgcgag cttgtcacgt gagttcaccg ctaaatggag
     2881 gtgaacatag ggcatgatga tggagacccg gtcgagtagc cggatcaacg cgtcgaggac
     2941 gctgtccgca tccgccgtga catctgacga tgactcgcgg ctcgtaattt cctcgacaag
     3001 ccttgagaaa gaagccgtg
//
LOCUS       DASYWM010000257         4364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_274226, whole genome shotgun sequence.
ACCESSION   DASYWM010000257 DASYWM010000000
VERSION     DASYWM010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274226"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VGR29_10465"
     CDS             <1..636
                     /locus_tag="VGR29_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2074051.1"
                     /translation="GGDPVISGEAWTTSAVNLRSGPSTGHQVLRVVPNGAKIGVSTTV
                     QNGFRYVNDQGQTGWMADAYIGSTSDGSQPDPVISGEAWTTSAVNLRSGPSTSNQVLR
                     VVPSGANIGVSTTERSGFLYVSYQGQTGWMSTAYIAYRDGGSQPGDDQGGNYRTTTTA
                     LNLRAEPSTSAKILTVMPAGAKVQLLHTGVGSFGNVNYNGMQGWAALEYLQ"
     gene            746..994
                     /locus_tag="VGR29_10470"
     CDS             746..994
                     /locus_tag="VGR29_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074052.1"
                     /translation="MCVNQYQHEHQVKIVISTYHHDAIQAENVRLAHTDQRRRTRAVE
                     TLRTAVRSLFTPAGDHMSQQLVSRPALVNTPSDKVLIA"
     gene            complement(1157..1381)
                     /locus_tag="VGR29_10475"
     CDS             complement(1157..1381)
                     /locus_tag="VGR29_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074053.1"
                     /translation="MTVQRLKELLAILMIGDGVLALIAPSRHPTLWKFGPRGYQQLMQ
                     AFSDHPTMKRFSGIGQIGLGVWLASRQWPR"
     gene            complement(1469..2590)
                     /locus_tag="VGR29_10480"
     CDS             complement(1469..2590)
                     /locus_tag="VGR29_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074054.1"
                     /translation="MHHAAPLGIMPLRHPRRVTMIVAVALALMPLTASAHVRWFTPEG
                     PYWDPDWGHIFSLQTLLALLSIGAIVTLLALGQRLIGDPLWPRPPFLQRMEPAAPAIL
                     GVQAAIALIYAATQLDLFVPNIELPENVFGVAIAGVAIAAGFTFITGVLTRVGALLII
                     GLFVLAFFFGAWYEALEQVIFVGIALYLVAVGRGVVRYDSGTEEDRTAFSDRLLPNAL
                     PILRWCAGISILVLGFTEKLLAPDLGVAFLQEYPNFNVLREFGLEWFTDRRFVYMAGI
                     VEATAGAALLCGFLPRVVILSLWIPFNLGIPFLPPTELIGHLPILATMYVLLVRGTEG
                     IPPRTAPAGTQPDRETPVDTSQRAATESAPPRHPAGATP"
     gene            complement(2827..3735)
                     /locus_tag="VGR29_10485"
     CDS             complement(2827..3735)
                     /locus_tag="VGR29_10485"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2074055.1"
                     /translation="MANALLPTRSLGATGVIVPVLGYGTASLGKSHVTREHAEQCLNH
                     AIDSGITYLDTSPDYGSEPHVGAVMRTRRNEVFLATKVNRRSQDGVLDELKESLEKLQ
                     TDHVDLIQVHAVNAWADLEQVLAPDGTVAGLLEAREQGLVRFIGITGHARPELLGYAL
                     ERYEFDTVLVALGMADRLVTSPETFVLPRAQERNIGVIAMKVFGHGTFTSRHLALRYS
                     LGLPGVSLAIVGMDTTDHIDENVGLTRDISPLSIDEEQQLVDEVRPLVERDAAESQEG
                     QSDLFWLHDTKVMGWQEHDEPALVRY"
BASE COUNT          906 a         1402 c         1291 g          765 t
ORIGIN      
        1 ggtggggatc cggtgatctc cggtgaagct tggaccacct ccgccgtcaa cctccgctcc
       61 gggccctcaa ccggacacca ggtcctgcgg gtggtaccca atggtgcgaa gatcggcgtc
      121 agcactacgg tgcagaacgg cttccggtat gtcaacgatc aggggcagac cggctggatg
      181 gccgatgcct acatcggctc caccagtgac ggctcccagc cagacccggt gatctccggc
      241 gaggcgtgga ccacctccgc ggtgaatctg cggtcgggcc cgtcgaccag caaccaggtg
      301 ctccgtgtcg tcccaagtgg cgccaacatc ggcgtgagta cgacggagcg aagcggcttc
      361 ctctacgtca gctaccaggg ccagacgggc tggatgtcca ccgcgtacat cgcctatcgc
      421 gatggcggtt cccaaccagg tgacgaccaa ggcggcaatt acaggacgac gacgaccgca
      481 ctgaacctgc gcgccgagcc gagcacatcg gcgaagatcc tgacggtcat gccggcgggt
      541 gccaaggtcc aactcctgca cacgggagtc ggaagctttg gcaatgtcaa ctacaacggc
      601 atgcagggat gggccgccct cgaatacctg cagtagatcg ccctgatggg agcgggctgg
      661 tcagcccgct cccaccccaa tcatggaacc gtctcaacac atccacttgt cggcaaaaac
      721 aggacaccaa aaggaacaaa gcaccgtgtg cgtaaatcag taccaacatg agcatcaggt
      781 caagatcgtc atttccactt accatcacga cgcgatccag gccgagaatg ttcggctcgc
      841 ccacaccgat caacgtcgac gcacccgcgc agtcgaaacc ctccgtaccg cagtccgctc
      901 gctgttcacc ccggccggtg accacatgag ccaacagctg gtctcccgcc cagcgctggt
      961 caatacgcca tccgacaagg tgctcatcgc ctgaccgcat atgcggcagc tggctcaagg
     1021 ccagccctac ttccacgcag aacctccgct gccttcgacg agaccactcg ttcaactcag
     1081 cggaggtttt tctgtacacc gtctcatatt gagaacccgg attgcagcag ttcagcactc
     1141 tgcctttgcc agcgcctcaa cgaggccact ggcgagatgc cagccaaaca ccaagcccta
     1201 tctgtccaat gccggagaat cgcttcatgg tcgggtgatc gctgaacgcc tgcatgagtt
     1261 gttgatatcc tcgcggaccg aacttccaca gcgtaggatg gcggcttggc gcgatcaggg
     1321 caagcactcc atctccgatc atgaggatgg ccagcagttc cttgagtcgt tgcacagtca
     1381 tctcgttctc tcctctttct cttaaacctg ttgcgatggg tccactgcca gggcagcgag
     1441 aataagctgg taccggggac cgcaacactt acggggtggc acccgcggga tggcggggcg
     1501 gtgcactctc agtggctgct ctttgcgacg tgtcgaccgg cgtttcgcgg tccggctgcg
     1561 tcccggcagg agcggtccgc ggcggaatcc cctcggtgcc ccgaaccagc agcacgtaca
     1621 ttgtcgccag gatcggcagg tgaccgatca gctcggtcgg cggcaggaag gggatcccca
     1681 gattgaaggg gatccagagt gaaaggatga cgacacgagg caggaagccg cagagcaggg
     1741 ccgcgccagc ggtcgcctcg acgatgcctg ccatgtagac gaagcgccgg tcggtgaacc
     1801 actccagacc gaactcccgc aacacattga aatttgggta ttcctgcagg aaggcgacac
     1861 cgagatctgg cgccagcagc ttctcggtga agccgaggac gaggatcgag atgccggcac
     1921 accagcgcag gatcggcagg gcatttggca ggagacgatc ggagaaggca gtgcgatcct
     1981 cttccgtgcc gctgtcgtaa cgcaccacgc ccctacccac tgccactagg tagagggcga
     2041 tgcccacgaa gatgacctgc tccaaggcct cgtaccaggc accgaagaag aaggccagca
     2101 caaaaaggcc gatgatgagc agtgcaccga cccgcgtgag gacgccggta ataaaggtga
     2161 aacctgctgc gatcgcaaca ccggcgatgg ctacaccaaa cacattctct ggcagctcga
     2221 tattcggcac aaagagatcg agctgcgttg ccgcatagat gagcgcgatt gccgcctgga
     2281 caccaaggat cgccggcgcc gcaggctcca tgcgctggag gaagggtggc cgcggccaga
     2341 gcggatcgcc gatcaagcgc tgccccagcg cgagtagcgt cacgattgcg ccgatcgaca
     2401 gcagggcgag cagtgtctgg agcgagaaga tgtgccccca atccggatcc caatacggtc
     2461 cttcgggcgt gaaccatcgg acatgcgcgg acgcggtgag cggcatcagt gcaagtgcga
     2521 ctgcgacaat catcgtcacg cggcgtggat ggcgaagtgg catgatcccc agtggggcgg
     2581 cgtgatgcat gtgtaacctg tcctcaacgg gcatggaagc aatcatcggc ggccgagcat
     2641 ccggcacgct ccctcactcc ccttgcagga tggatgccgg gtagcggttc ggcaacacaa
     2701 aaggggcggt caagcgaccg ccccttcttg cttcacctcg gatgatggcg agcgcgtaca
     2761 tcatctggtc cctggaccca tgccccggcg ccgggttaaa ctggcctcac gcgttcacac
     2821 cgggcgctag tacctcacca acgccggctc gtcgtgctcc tgccaaccca ttaccttggt
     2881 atcgtgtaac cagaagagat cgctttggcc ctcctggctt tccgcggcat cacgctctac
     2941 cagtggccgg acctcatcca cgagttgctg ttcctcgtcg atgctcaacg ggctgatgtc
     3001 cctcgtgagg ccgacgttct cgtcgatgtg atccgtcgtg tccatgccga caatcgccag
     3061 cgataccccc ggcagcccca gtgaatagcg gagcgccaga tgacggctcg tgaaggtgcc
     3121 atgcccgaag accttcatgg cgatgacacc gatatttcgc tcttgcgccc tgggcaggac
     3181 gaaggtctcc gggctcgtca ccagacgatc ggccatgcca agggcgacga gcacggtatc
     3241 gaactcgtag cgctccagcg cataacccag taactccggc ctagcgtgcc cggtgatgcc
     3301 gatgaagcgc acgagtccct gctcccgcgc ctccagcaac ccggcgaccg tgccgtccgg
     3361 ggcaagaacc tgctcgagat cggcccacgc gttcaccgca tgaacctgga tcaggtcgac
     3421 atgatcggtc tgcagcttct ccagggattc tttcagctcg tcaagcaccc cgtcctggct
     3481 gcgccgattc accttggtgg cgaggaagac ctcattgcga cgtgtccgca tcaccgcgcc
     3541 gacgtgcggc tcgctgccgt agtcggggga ggtgtcgaga taggtgatcc cgctatcgat
     3601 cgcgtggttc aggcactgtt ccgcgtgctc acgggtaaca tgcgacttgc cgagtgaagc
     3661 ggtcccgtag ccgagaacgg gcacgataac accggtagcg ccgagcgatc gggtgggaag
     3721 aagcgcgttg gccatgggtt gctccatttc aagaaatgcg ctgacgcctc atgcacagga
     3781 gcggggacac gtcgtccggg ttctgcgtaa cggaccatgg agcaagcgca atgccatcaa
     3841 tcggagtgga ctgcgcctcg gcccggatgg agactcgcaa gagcgcggca cagtggtcca
     3901 gcccataaca caaggcgggg aatgagcatc caatcagctt atggaggcgc acgttcacca
     3961 agagggctac cggcttcacg gacgtgtagg gaacgcaatc gtcactctca aacacattga
     4021 gggatatatc gcgctcccac attccatgcc cgccccggat tctgcttgtc catccgtcga
     4081 accccgagca cgtccaagca ccctgccgta gggataatta gctcccgtcc cggcgcactc
     4141 catttccatg cccgcgtcat tcatccgcga gtgtcgaact cccggccagc gttgcagctg
     4201 gttcaaggac tctggccgga tgccctcttg tgcctgtgga ctcatcgtat ctaccttgcc
     4261 cgtcacacca ggatgaatat ccaaacagca ctgtggtcga ggctagaaac ctgcagtctt
     4321 gccgatgatc cacgctgctc tcgaccagcc gaaatctgaa cttc
//
LOCUS       DASYWM010000258         8790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_367861, whole genome shotgun sequence.
ACCESSION   DASYWM010000258 DASYWM010000000
VERSION     DASYWM010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367861"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VGR29_10490"
     CDS             complement(<1..1293)
                     /locus_tag="VGR29_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074056.1"
                     /translation="MSRVLIEEEIETSARPSLWRRADVIAIAVLLVLTLIVMWHRWIF
                     DNWLARHDLLTFFLPWLGSLGDRLRALDIPALNPYIFSGAPFAGDPESGWMYVPAMLV
                     FPFFEVTVAYKLMILLLLVLAGTSTYALARVIGYSVLAALFSAVAFEFGPFLFGQTDC
                     CTVGTKSTAFFPMAFLGVELALRARTWPIRLASWVIAGFAISQMFAGWLGQGVVNALV
                     LIAAWVVFRALITPPEIGWNLKERITQMVTTGTAILVLGLLLGAAGILPRLAANAESN
                     NPGGTYENIPGARAANFHTLASALKTIMVDSLAFRGSSVWGFVLVLMVLAIFLTRRRS
                     IIPFFALAAVVVPALAMGVPVLSDLFYLIPRYESMHSHSPGRVFWLYPFIPAMLAGAA
                     VNELPRLRSMRGRWIIAVIPLVVLALAARHVERVEKVEV"
     gene            complement(1482..4628)
                     /locus_tag="VGR29_10495"
     CDS             complement(1482..4628)
                     /locus_tag="VGR29_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2074057.1"
                     /translation="MATDGTSGTAPGTPRYRNVSGKPLNRRVVLKAAAGLSAIALTGA
                     AATVGTSRPERISAQEIDGTRAREWVAEDHIGVDVEADRDGWVTFSAEFPFWAVGIGW
                     AGDVGTWPVIELQVSYDGAEWGDVWFMTARVDDGGRQSIDGRLFTDLLLTDGQEFIRY
                     RTLDGEGNLSPVDGLVVSYIDPTDGPWAEDRGDTMMRISVAEVNDDTDAPPSIITRAK
                     WGANEDYRYDSFGEIWPPEYETVEHAIVHHAAVNYGSDGYNAVRSIYYYHAITQGWGD
                     IGYNYLVDPTGKIFEGRVGGQNVIGGHSFEYAIGSSGICVIGDFSYQDATEAAKAALV
                     HILAFVTRDLETYATKPFHEIPNLPTICAHRDVNQTSCPGDFLYADLPEIRDLVAATL
                     DAGTLNTGNPAGIVPGDQVKVQTDDGAPLNMRSGAGLSSPVNGSIPNGTLVPVLDGPI
                     SDPNDNWYKVRYDGLDGWVSARYLIVTPPPPPFTEADYQFGTNMRFTTDANVRREPST
                     SSSVLGVATQNTWTVIMAGPIVADSYTWYQIRVPGIGDGWVAKDFIHPAPVDNNPAAK
                     FKVGDTVSAIRSTSVRARPGIAQTVASTASAGTTMQVSVAPLAVTGYIWYGVYSPSFG
                     GGWVAENDIQGATTTPPRTGDLVAGDTVRVTETLNMRSGPSTGNGIIAVLPTGTTGRV
                     LAGPSTGSGYTWYQIQTSSGSGWVVRDWIAKTTTAPPPAGKFVVGDTIRVTDTMNMRS
                     GAGTNFGIIASLPTGTAGTVLDGPRAASGYTWWQLRTPVGTGWAAENWLEKTTTAPPP
                     DPQPEPQPTPGGHPAGTQLRVTDALRLRSAPNTSGGVIATMPTGTTVTVVSGPTSSGG
                     YTWYQLTTPYGNGYAAGEFLQPTGTTTPTDPPPTKPPPPTSGKFAIGATVRVTEALNM
                     RSGPGTGNGVIATLPAGTTGTVLEGPSTGSGYTWWRIQTSQGTGWAAENWLAAASGSP
                     TPPPSGAFATGDRFQVTEALNMRSGAGTGNGIVATLPAGTTGTIAGGPTSASGYTWWQ
                     VQTSSGNGWVAQDWIRKI"
     gene            complement(4816..6720)
                     /locus_tag="VGR29_10500"
     CDS             complement(4816..6720)
                     /locus_tag="VGR29_10500"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEV2074058.1"
                     /translation="MVQPTTTHESGGVSGRFSGAMRFLQGEGMTAPDWSRTARRIGPR
                     LVAGAATLLLILAIGLLVFRVMFDDKIYPAVVVGDVEVGGLTMDQAQERLSDRARDLN
                     LNSITFSYGEKAWTPTLTELGATIRTDAALEQAHSLGREDNAVSRLAFTGNILQDDQT
                     VPLETSLDPTRLDAWFDTVDADIGNPAINAGIVVDGANVSFTPDSTGIVVNREAAKAQ
                     ILTVLQTLQPVDAELPTVVDEPELRVADLEAGKAQVENVLSQPVQIRFEDQTWQVQGT
                     EISQFLRVTSQMSDGKPRTVIDFDRDGLSSYLRETYAGQINRTPVDATVAFSDGLYAT
                     SPSVDGAAVMTNEFADVVAGSFLNGHEPVDVPVHVTKPKVDSNNLAALKIETLIGRGD
                     SNFSGGSEERDHNIYTGIKGVDGTLVAPGEDFSFNGAVGAITPEAGYVESSVIVGEAI
                     GRDVGGGICQVSTTVFRAALMAGFPMPEWYPHTYRLGGYERDGWGPGFDASILQLGDN
                     PANWADYKFTNTTDGWILVQAWTSYPYAVVEIFGHDMGWNVELSEVTQSGPIKGEDME
                     VVDGTLPPGSIVEYAAPLDGLSVGFQRIVTDANGEVISDRWFQTDFAGRGIQYNVSPD
                     MAGKSPGAEG"
     gene            7333..8736
                     /locus_tag="VGR29_10505"
     CDS             7333..8736
                     /locus_tag="VGR29_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2074059.1"
                     /translation="MSRNKTFSHSLSRRRLVGSSLAAAAASTAFASPAVIGAASRQEG
                     TPAGTASGELVEWGFGIQETNPLARARVLAFQEAFPDVNLQIVESFDEQKLLTAAASD
                     TLPDVIWLSRAETATWAAPGVIRPLTEFIERDGYNTAEFYPFAIEEASYEGEVYGIPG
                     GADVRALYVNLDHLEEIGVDVESLDTSDWEQLSELGSQLVQREGDQVQRWGFDHRVQA
                     NNFWLWGYGNGGQFLADDATEATFDSEANVEALQWAVEAYEAQGGSNLYDAFATTFQG
                     DQQFANGSVSMSVYEQWMLSAAIATVAPDMNFRLLPIREWGSGPEGSIATYSGGNGWY
                     ITSGAKNPEAAWEYIKFLHTDETWMIGARAVKALREANNNPYFPSLTGKPEVDQMQID
                     ELYEPVSEAFNSAVDLLPTLLRESTARELYRSPVGGQLEDILLADAIKPAMSGSETPQ
                     DALENADQNAQDAIDMF"
BASE COUNT         1749 a         2723 c         2647 g         1671 t
ORIGIN      
        1 gacctcaact ttctccacgc gctctacgtg ccgggcggca agtgccaaga ccaccagcgg
       61 gatgaccgcg atgatccatc tgccccgcat cgatcgcagc cgtggcaatt cgttcaccgc
      121 agcacctgcc agcatcgcag ggataaatgg gtaaagccag aagacacgcc cagggctgtg
      181 gctgtgcatg ctctcatagc gtgggatgag atagaagagg tccgagagta ccggcacccc
      241 catggcaaga gcaggcacga ccaccgcagc aagagcaaag aaggggatga tcgagcgtcg
      301 ccgtgtcagg aagatggcca gcaccatcag cacgaggaca aatccccaaa cagagcttcc
      361 cctgaacgcc aggctgtcta ccatgatcgt cttcagcgcg cttgcaagcg tatggaaatt
      421 cgctgcccgg gcacccggga tgttctcgta ggtgccaccg ggattgtttg attccgcatt
      481 tgccgcgagt ctgggcagaa tccccgcagc gccgagcagg aggccgagaa caaggatggc
      541 cgtccctgtc gtaaccatct gcgtgatccg ctctttcaga ttccagccaa tctctggcgg
      601 cgtgatgagg gcacggaaga ccacccaggc ggcgatgaga acaagcgcgt tgacaactcc
      661 ctggcccagc catccggcga acatctggct gatcgcaaat ccggcaatga cccacgaagc
      721 gagacgtatt ggccacgtcc gtgccctgag cgccaactca acgccgagaa acgccatggg
      781 gaagaaggcc gtgctcttgg tgccaaccgt gcagcaatcg gtttgcccga agaggaaggg
      841 gccgaactcg aacgcaactg cagagaacag cgctgccagc acgctgtatc caatcacgcg
      901 cgcgagggca tacgttgacg tacctgcaag gaccagcagg agcaggatca tcagcttgta
      961 cgcgacggtg acctcgaaga agggaaagac gagcatcgcc ggcacgtaca tccagccaga
     1021 ttccggatca ccggcgaagg gagcaccact gaagatgtag gggttcaatg ccgggatatc
     1081 gagtgcgcgc aggcgatcgc cgagggatcc cagccagggg agaaagaatg tgagcagatc
     1141 gtggcgtgcg agccagttgt cgaagatcca tcgatgccac atgacgatca gggtgagcac
     1201 cagcagcact gcgatggcga tcacatccgc acgacgccac agcgatggtc gggctgacgt
     1261 ctcgatctcc tcttcaatca acactcgcga cattctccga tcgttcctgt tcttctcgca
     1321 cccgacctga tgctgctcct ggcgacggcc gggatccgtt gcccgggatg ccggagcgct
     1381 tgcactccat acggtgtccg cgtgacgccg atgcaagact cttaccacat aacaacagcc
     1441 gggcgcggtg cccggctgtt gttggtgact agcgtgccag cctagatttt acggatccag
     1501 tcctgggcga cccacccgtt gcccgacgag gtttgcacct gccaccaggt gtagccactc
     1561 gccgaggtcg gtccaccggc aatcgtcccg gtcgtgcccg ccgggagcgt tgcgacgatg
     1621 ccgttgccag tgccagcacc ggagcgcatg ttcagcgcct cggtgacctg gaatctgtca
     1681 ccagttgcaa aggcaccact tggcggcgga gtcggcgaac cggacgccgc cgcaagccag
     1741 ttctcggcgg cccatcccgt tccctgggag gtctggatgc gccaccaggt gtagccgctt
     1801 cccgtgctcg gcccctcaag caccgtaccg gttgtgccgg ccggcagcgt ggcaatgact
     1861 ccgttgcccg tacccggccc cgagcgcatg ttcagggctt ccgtcacccg gaccgtcgcc
     1921 ccaatggcga atttgccgga tgtcggcgga ggcggcttcg tcggtggcgg atcagtgggc
     1981 gttgtggtcc cggttggctg gaggaactca ccagcggcat agccatttcc gtacggcgtc
     2041 gtgagctgat accacgtgta acctcccgac gacgtgggcc cggaaaccac ggttaccgtc
     2101 gtaccggttg gcatggttgc tatgacacca ccgctcgtat tcggtgctga ccgaagcctc
     2161 agtgcgtcgg tcacacggag ctgagtgccg gccggatgtc ctcccggggt gggttgaggc
     2221 tccggctgcg ggtctggcgg aggcgcggtg gtcgttttct cgagccagtt ctcggctgcc
     2281 catcctgtcc cgaccggcgt cctgagctgc caccaggtgt agccactggc cgcccgggga
     2341 ccatccagta ccgtgcctgc tgtaccggtg ggcagcgagg caatgatccc gaagttcgtg
     2401 ccggcaccgg aacgcatatt catggtatcg gtcacgcgga tcgtatcgcc gaccacgaat
     2461 ttgccggctg gtggcggtgc ggtcgttgtc ttggcgatcc agtcacgcac aacccatccg
     2521 gaacccgagc tcgtttggat ctggtaccac gtatacccac tgcctgtgct tggacccgcc
     2581 agtacccggc cggtcgtacc ggttggcagg accgcaatga tgccgttgcc agtgcttggg
     2641 cccgagcgca tattgagcgt ctctgttacg cgaaccgtgt ccccggcgac aagatcgccg
     2701 gttcgaggag gagtggttgt ggccccctgg atgtcgttct ccgcaaccca gccgccgcca
     2761 aagcttgggc tgtagacgcc ataccagata taccccgtga ccgccagcgg cgcgaccgag
     2821 acctgcatgg tggtgcctgc actcgccgtg gatgcgacag tctgcgcgat gcctggacga
     2881 gcacggacgc ttgtcgagcg gatcgcggag actgtatcgc cgaccttgaa tttcgcggcc
     2941 gggttgttgt ccaccggagc ggggtgaata aagtccttcg cgacccaccc gtccccgatg
     3001 cctggtacac ggatctgata ccacgtgtag ctgtcggcga cgatggggcc cgccatgatg
     3061 accgtccacg tgttttgcgt cgccacgccg aggacggagc tgcttgtcga cggctccctg
     3121 cgaacattcg catccgtggt gaagcgcata ttggtgccga actgatagtc cgcttcggtg
     3181 aatggaggag gcggaggggt cacgatgagg tatcgcgcgg agacccagcc atccagcccg
     3241 tcgtaccgga ccttgtacca gttgtcgttg ggatccgaga tggggccgtc aagcaccggc
     3301 accaaggttc cattagggat ggaaccgttg accgggctgc tcaggccggc accgcttcgc
     3361 atgttaagcg gggcgccgtc gtcggtctgc accttcacct ggtcgcccgg gacgataccg
     3421 gcaggatttc ccgtattcag cgtgccagca tccagcgtgg ccgcaaccag gtcacggatc
     3481 tccggcaggt cagcgtagag gaagtcaccc gggcaggatg tctggttgac atcgcgatgt
     3541 gcgcagatgg tcggcaggtt tgggatctcg tggaagggct tggtcgcgta ggtttccaga
     3601 tcccgcgtca caaaggcgag gatgtggacc aacgcggcct ttgccgcctc ggtggcgtcc
     3661 tggtagctga aatccccgat gacgcagatg ccagagctgc cgatggcgta ctcgaaggaa
     3721 tggccgccga tgacattttg gccgccaaca cgcccctcga agatcttgcc ggtcgggtca
     3781 accaggtagt tataaccaat gtcgccccag ccctgggtga tggcgtgata gtagtagatc
     3841 gaacggacag cgttgtatcc atcagagccg tagttgaccg cagcatgatg gacaatggca
     3901 tgctcgactg tttcatattc cggcggccag atctcgccga aggaatcgta tcggtaatct
     3961 tcgttggcgc cccactttgc ccgggtgatg atcgaaggcg gagcgtccgt gtcgtcgttg
     4021 acttcagcca cggagatacg catcatcgtg tccccgcggt cctccgccca gggaccatcg
     4081 gtcgggtcga tgtaggaaac caccagaccg tcgaccggag agaggttccc ttcgccatcc
     4141 agggtgcggt aacggatgaa ttcctgacca tcggtcagga ggagatcggt gaagagacgg
     4201 ccgtcaattg actggcgacc accgtcgtcg acgcgcgcgg tcatgaacca gacatcaccc
     4261 cactcagcac cgtcgtagct gacctgaagt tcaatgaccg gccaggtccc gacatcaccg
     4321 gcccaaccga taccaaccgc ccagaacggg aattcggccg aaaatgtgac ccatccgtca
     4381 cgatccgcct cgacatctac cccaatatgg tcctcggcca cccactcgcg tgcgcgggtg
     4441 ccatcgatct cctgtgctga gattctctcg gggcgcgacg tgccgaccgt tgcagccgct
     4501 ccggtcagcg caatggcact gagcccggca gcggctttca ggacaacacg tcgattcagt
     4561 ggtttgccgg agacattacg ataccgaggc gtgcccgggg ctgtaccgga cgtcccatcc
     4621 gttgccatga tgtagtctcc ccctcccgga gatgcttagc tcttctgcgg ccgaagctgt
     4681 gatttgttcc tggcccgcct ggaaataccc cgagagagcc gtgtcccgca tcaagtttac
     4741 cgtgcaacga cacccctcat gtcttcctgt gtcatgaggg gtgtcgtgca ggtccggcga
     4801 tcactactgg taggtctaac cttcagcacc gggggacttg cctgccatat caggacttac
     4861 attgtactga atgccacgac cggcaaagtc cgtctggaac cagcgatcac tgatcacttc
     4921 accattcgca tcggtcacaa tgcgctggaa cccaaccgag aggccatcca acggtgccgc
     4981 gtactcgaca atggatccgg gcgggagcgt tccatcgacc acttccatgt cctcgccctt
     5041 gatcggaccg ctttgggtca cctcggagag ctcgacattc caccccatgt catggccgaa
     5101 tatctcgacg acggcgtatg gatatgatgt ccacgcctgc accaggatcc agccatcggt
     5161 ggtgttggtg aacttgtaat ctgcccaatt tgccgggttg tcgcccagct gcaggatcga
     5221 ggcgtcgaaa cccggccccc aaccgtcacg ctcgtatcca cccaagcgat aggtatgtgg
     5281 ataccactcc ggcatgggga agccagccat gagggcagcg cggaacacgg tggtcgaaac
     5341 ctggcagata cctccaccaa cgtcgcggcc gattgcttca ccaacgatga cgctcgattc
     5401 cacatacccg gcctccgggg tgattgcgcc aacagcgcca ttgaacgaga aatcctcgcc
     5461 cggggccacc agtgtgccgt ccactccctt gatgcccgtg tagatattgt ggtcacgctc
     5521 ctctgagccc ccactgaagt tcgagtctcc ccggcctatg agggtctcga tcttgagcgc
     5581 ggcgaggttg ttggagtcca ccttcggttt cgtgacatgc accggcacat caacaggttc
     5641 gtgaccatta aggaagctcc cggcaacgac gtcggcgaat tcgttggtca tgacggcggc
     5701 gccgtcaaca cttgggctgg tcgcatacaa gccatccgaa aacgccaccg ttgcgtcaac
     5761 gggggtacgg ttgatctggc ctgcataggt ctcacggagg taagagctca gcccatcccg
     5821 atcgaagtcg atcacggttc gcggcttgcc gtcggacatt tgcgaggtaa cccggaggaa
     5881 ctgggagatc tctgttccct ggacctgcca agtctgatcc tcgaaccgga tctgcaccgg
     5941 ttggctgagg acgttctcga cctgggcctt gccagcttcg agatcagcga cgcgcagctc
     6001 cggttcatcg acgaccgtgg gaagctcggc gtccactggc tgcagcgtct ggaggacggt
     6061 cagaatctgc gctttcgccg cctcacggtt gaccacgata ccggtcgaat caggagtgaa
     6121 ggagacgttc gcgccatcaa ccacgattcc ggcattaatg gcaggattcc cgatatcagc
     6181 atcgacagtg tcgaaccagg catcgagccg ggtaggatcg aggctggttt ccaacgggac
     6241 cgtctggtca tcctggagaa tattgcccgt gaaggccagg cgggacaccg cgttatcctc
     6301 tcggcccagg ctgtgcgcct gctccaatgc agcatcggta cgaattgttg caccaagctc
     6361 ggtcagcgtt ggcgtccagg ctttttctcc atacgagaag gtgatgctgt tgaggttgag
     6421 gtccctggcg cgatctgaca ggcgctcctg cgcctgatcc atggtgagac caccgacctc
     6481 cacatcgcca acaacgacag cgggataaat cttgtcgtca aacatgacgc gaaaaacgag
     6541 gagcccgatt gcaaggatca aaaggagcgt ggccgctccg gcgaccaggc gtggcccgat
     6601 ccgtcgcgcg gtcctggacc agtccggggc ggtcatgcct tcgccctgca agaagcgcat
     6661 tgcgccggag aatcgtccgc tcaccccgcc ggactcatgt gtcgttgtcg gctgcaccat
     6721 gtgtattccg ttaccccaaa actgattcga tgacacagat tgatcgcagc aacgcatctc
     6781 gccagcgata tcgatcccca cgaccggcaa tcacgcgcat gatattgaac tgttgtagtc
     6841 gatggaacga accccgcccc aagcggcagg tgggccgccc gccccgcagt gaactccctt
     6901 aaaatagcat atgccgatct cgcacatgtc tcccaccgct tcgacaggtg ctgacgtctc
     6961 tgcagtaata aacgtccttg tgatgatatt ggactcatac cgagttcccg ggaaatgcgc
     7021 agctcagtgt ggttagggtc cgccgtctga tacagactgg cggcggcgag gtatcgctgg
     7081 ccagtgcgct ctgcggagca acgatgtgcc gtgccccgca tgcttaatta tgaaagaaca
     7141 tccctaaatc tgatagtatg aagcccagag agacaacata atcgggtggc atcgtttctg
     7201 cttctacttg tggtacgacg ttccaggtgc atgactcagg ctccgcaagg cgagagattc
     7261 ggttgtgtcc gcttctaaag gaacgtacgt acatacgcta tacgcacttt atgagtgcag
     7321 gaggtttact caatgtcgag gaacaagaca ttctcacatt cgctgagtcg tcgtcgcctg
     7381 gtcggcagca gtctcgccgc ggctgccgca tccaccgcct tcgcctctcc cgcagtgatt
     7441 ggagctgcct ctcgccagga aggcaccccc gcaggcaccg cgagcggtga gctggtcgag
     7501 tggggattcg gcatccagga gaccaacccc ctggcgcggg cacgtgttct cgccttccag
     7561 gaagccttcc cggatgtgaa cctgcagatc gtggaatcat ttgacgagca gaagcttctc
     7621 accgccgcag cgtctgacac gcttcccgat gtgatttggc tcagccgggc ggagaccgcc
     7681 acctgggccg ctcccggggt tatccgccca ctcaccgagt tcatcgagcg cgatggctac
     7741 aacactgcgg agttctaccc cttcgcgatc gaagaagcct cctacgaggg tgaggtttac
     7801 ggcatcccgg gtggcgctga cgtccgcgcg ctctacgtca atctcgacca cctcgaggag
     7861 atcggcgtgg acgtcgaatc gcttgacacc tccgactggg aacagctcag tgaactcggt
     7921 tcccagctag tccagcgcga aggtgaccag gttcagcggt ggggattcga ccatcgcgtg
     7981 caggcaaaca acttctggct atggggctat ggcaacggcg gccaattcct ggccgacgat
     8041 gccacagaag ccaccttcga ttcggaggcg aacgtcgagg cgttacagtg ggcggtcgaa
     8101 gcctacgagg cgcagggcgg ttccaatctc tacgatgcct tcgcgactac cttccagggt
     8161 gaccagcagt ttgcgaacgg ctccgtctcc atgtcggtgt acgagcaatg gatgctcagc
     8221 gctgcgatcg cgacggttgc gccggacatg aacttccggc tgctcccaat ccgcgagtgg
     8281 ggaagcgggc cggaaggctc gatcgcgaca tactccggcg gtaatgggtg gtacatcacc
     8341 tccggcgcca agaacccgga agcggcctgg gaatacatca agttcctcca cacagacgag
     8401 acctggatga tcggcgcccg ggcggtgaag gcactccgtg aggcgaataa caatccgtac
     8461 ttcccgtcac tgaccggtaa gcccgaagtc gaccagatgc agatcgatga gctctatgag
     8521 ccggtcagtg aggccttcaa cagcgccgta gatctactcc cgaccctgct ccgagagagc
     8581 accgcgcgtg aactctaccg atcaccggtc ggtggccagc ttgaagacat cctgctggcg
     8641 gatgccatca agccagccat gagcggcagc gaaacaccgc aagacgcgct cgagaacgcg
     8701 gaccagaacg cgcaggacgc gatcgacatg ttctagtcga gaggacctgt tgcgcgggat
     8761 ggcctggaaa gcatgctttc caggccatcc
//
LOCUS       DASYWM010000259        23492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_218637, whole genome shotgun sequence.
ACCESSION   DASYWM010000259 DASYWM010000000
VERSION     DASYWM010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 23492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23492
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218637"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VGR29_10510"
     CDS             complement(<1..348)
                     /locus_tag="VGR29_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074060.1"
                     /translation="MTSHPPTNDNAMKVRQRAEGLLQQMTPAEKAGQLTQYFYFGSVS
                     DQESPDGSGPLPQPAVIEAALRRGEVGSLLFVTDPAETNRLQRIAMEETRLKIPLLFG
                     FDVIHGFRTVFPVP"
     gene            complement(406..1017)
                     /locus_tag="VGR29_10515"
     CDS             complement(406..1017)
                     /locus_tag="VGR29_10515"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2074061.1"
                     /translation="MMEDVPLPALIVVCGAPASGKTTLARRLAGNLRLPLLEKDVIKE
                     SLADAIGTQDGEDSRKLDYASTRLLYALATPMLRHGTSLMVECNFDRAFASADLNRMS
                     TTARVQIVQCWSDRETIVRRYRERSLNGQRHGAHFDLEALPDLIAGLDRDAYNLSTLG
                     YPAITVDTTDGLHPGFSAITGLVHAHLRQEPHGLSPTDTPPLP"
     gene            complement(1037..1915)
                     /locus_tag="VGR29_10520"
     CDS             complement(1037..1915)
                     /locus_tag="VGR29_10520"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEV2074062.1"
                     /translation="MSQRLGIVAAFSKPEAKELSTTVRQWLEARGHTTVAEPDILAGD
                     RRNVDAIIVFGGDGLMMRMANAFPDIPLFGINFGKVGFLALVEQKDWTAALQLFLDGK
                     YDIDESSTLQASIYRDDKEYDQGWAINDVVIRGGNRMIEDEVYIDGYYVNTYPGDGMI
                     VSTPRGSTAYCMAAGGPILTPGVRGFAVVPINCHSPIRTPMITSEEALIEIIHSNVHP
                     GYMILDGQQTMEVQKGDLIKIQRGEHTFQLIRVTITNFFDAIRTKFNFQVRPDAVPSR
                     LAIAPARDEADKVVSR"
     gene            complement(1952..2464)
                     /locus_tag="VGR29_10525"
     CDS             complement(1952..2464)
                     /locus_tag="VGR29_10525"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074063.1"
                     /translation="MDQTAVRIIREATGVDAQYLEQLYTFSPGHGPVREIVISYLGLF
                     SPDTPVTAGTIDLAWPLVKDAQPADGLDQMVLDYALIRLRAKLGYSNIAFYLVPSTFT
                     LSELQQTYETILGQPMDKRNFRRRMITSGILTDTRQKRREGSHRPAALYRFNLNDDQA
                     SYLTPPRHED"
     gene            2791..2861
                     /locus_tag="VGR29_10530"
     tRNA            2791..2861
                     /locus_tag="VGR29_10530"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2823..2825,aa:Gln,seq:ttg)
     gene            2918..4366
                     /gene="glmU"
                     /locus_tag="VGR29_10535"
     CDS             2918..4366
                     /gene="glmU"
                     /locus_tag="VGR29_10535"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV2074064.1"
                     /translation="MTNETAPSGVAPRVAVAVLAAGHGKRMRSQTPKHIHPVGGVPLV
                     ERIIRAGLSIQPARLLTVVSPAMAGMPAMLGMQGEFETVIQDPPRGTADAVRIALCEA
                     PEADYLVSLLGDNPLLTAEIVQTLVDGAITSDTKITILTCLLPDAQAYGRIHRDDLGR
                     VTSIIEAKNDHPSQRIGETEINSGIMVLDAAWAREALQQLPLDPVTGEYLLTDLVEMA
                     ASAHVEGEPWPIATVQAPVTVSLGVNNRLQQAEADALVRRQVRERLMHTGVSMIGADT
                     IFIDEQVQIGMDTVILPGTIIMGSTTIGCGCRIGPNTVLDHATIGDNVVIQSSTVEYS
                     MMESGSDAGPYAHIRGGSVIGEEVHVGNYAELKNTAMARGAKVGHFSYLGDATVGESV
                     NIGAGSVTANYDGTTKNRTVLERNVFVGSDTVFVAPVTVGEGAKTGAGSVVTRNVPPN
                     TTVVGVPARQIAKRSAEKSDGQEQDSGRTYKE"
     gene            4368..5303
                     /locus_tag="VGR29_10540"
     CDS             4368..5303
                     /locus_tag="VGR29_10540"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEV2074065.1"
                     /translation="MDGRLQVFAGNAHPALARAICEELEVDLGRAIVTQWKNGETRVK
                     LEENVRGSDVFVVQPTSCPVNHHLMELLLMIDALRRASATRITAVIPYYGYAKQEKKT
                     SGREPISAKLVANLLTVAGATRVLAVDMHSPAIEGFFDIPVDHLRATPLLARALRHDI
                     QHDIVVVSPDAGGVVRAEDFRERAKGSLAIISKQHPGADKTETVEMVGDVDGKIAVIV
                     DDMISTGGTLVQAAKLLKERGAAKIHVCATHGVFADDAIQMIDASSIDEIIVTDTIPL
                     PEHGKGPVKVVSVAPLLAEAIMRTHKGLSVSALFT"
     gene            5438..6811
                     /locus_tag="VGR29_10545"
     CDS             5438..6811
                     /locus_tag="VGR29_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV2074066.1"
                     /translation="MDADDWQHVIFAILALLLVAAAGVVLTIVGQATNQRLLPETADP
                     LHRGKRSTRSLVNPRRATASAMIFVQILATVIAASQITQLIDQWTHDDFNWTAILVVA
                     IVYIVFGQSLPRAIVDKQLTDQFLSRVLVVGRAVAQLLAPVSWLVVGLANLFRRVLPG
                     RDAEVEAIGTEDELSRSRLSPDDGVIGENERQMIDGILSLEEMTVRDIMVPRLDIVGL
                     ERTVSARELIETITKAGHSRIPVYRDTIDNVLGVLYVKDLLPFVIGNTIRIPLLDLIR
                     PAFVVPESKRLGELFTELKRTRIHLAVVADEYGGTAGLVTIEDILEEIVGEIQDEYDT
                     ESPLFEQGEDGFIVADGRLPIEDVEDALGVEFEEDDDFGTLGGFVHKHLGRLPIQGDT
                     FEAENVRVEILEVERHRVRKMRVTRLEVEDEEPADEVRERSDRWRRGGGSDTYDENAT
                     SVEDRLP"
     gene            6811..7599
                     /locus_tag="VGR29_10550"
     CDS             6811..7599
                     /locus_tag="VGR29_10550"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEV2074067.1"
                     /translation="MLGRPVIRFDAVASTMDVLADLAALGAPEGTIVIADYQSAGRGR
                     SGREWTAPPRTSLLYSILLRPELPIRQLTPLSLLVADAIVATLGECHGVQAEIKWPND
                     VLIKGKKVSGVLAQVKSQVDGKVTILGVGVNANILTADLPSNATSLLVELGEPVDHNA
                     LMWRFLNELESRYQDLLSSRIEQRWLAIRNTLAMLGDSVTVKERHADIHGILQGIDDE
                     GALLLDQGGVIRRVVAGDLTRGPRPDEPPDAETATSLPRILPRG"
     gene            7642..8844
                     /locus_tag="VGR29_10555"
     CDS             7642..8844
                     /locus_tag="VGR29_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2074068.1"
                     /translation="MNFELTEEQVQVRDMVREFAEREAAPHVREWDAKQNYSRDIIDK
                     MGESGILGLPIPEEYGGLGLDYVSLALACEEFEYVDTSLRVVLSVHIGLNSLSLLQWA
                     NDEQKQRFLVPQATGEKIATFGLTEPGAGSDAGAIETTATRDGDDYILNGSKMWISLS
                     DIADNFLVFASLDRSQKHKSLVAFVVERGMEGFTTGTIKGKLGVRAGNTGEMAFDNVR
                     VPGANRIGEEGEGFKIAMSAIDQGRFTVGAGAVGLTRAALDASVRYANDRQTFGVPIG
                     KHQLVQQMIAKMVAGYEASQLLVYKAAELKNRGIRNTRETSLAKWYATEAALQAADDA
                     VQIHGSYGFANDYPVERYLRNARGSTIYEGTSQIHQILQAEYALGYRIDKPLSRPQPP
                     AVGFEDEV"
     gene            8967..10148
                     /locus_tag="VGR29_10560"
     CDS             8967..10148
                     /locus_tag="VGR29_10560"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV2074069.1"
                     /translation="MGRSVILGGARTPFGKLNGALASLTAVELGKVAAWAAIDRAGIE
                     DDAINHVIMGQVLQGGAGQNPARQVGLGLGLDRTVTAETINRVCGSGLRSVALADMQI
                     RLGESAVVLAGGMESMSNAPYLLRGARAGYRMGDGELVDMMMHDGLRCAIEGCSMGFH
                     GGRVSDEERISREEQDAWALRSHQRALAAIDDGRMAEEIVPVEVAQKKGTTIVDTDES
                     PRRETSAEALAKLKPAFQKDGTVTAGNAPGINDGAGAVVVADEIWARQRGLTPRATIL
                     ATGQSAWDAPYLAYTPEMAIRNALMKTEHTLEDIELFEINEAFASVAIIASKRLGIDP
                     DKLNVTGGAVALGHPIGASGTRLVITVINELTRRGGGLGAVAICSGGGQGDAMILKVE
                     A"
     gene            10148..10744
                     /locus_tag="VGR29_10565"
     CDS             10148..10744
                     /locus_tag="VGR29_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEV2074070.1"
                     /translation="MDRRAEFSARFPGAIILPFEDAWPAIADDALIAPGAVLIGSVTI
                     GSESSVWFNTTIRGDIAPVTIGARTSVQDNTVVHVNMDAATTIGDDVTIGHSALVHGT
                     TIHNGALIGMGSLVLSYSVIGRNAVVAAGALIPERFYVPDGAVMVGVPAKQRDALSDE
                     QAERLDGISGRYVRVSRTYRETVMMLPSGEEEEPEGEH"
     gene            10734..11534
                     /locus_tag="VGR29_10570"
     CDS             10734..11534
                     /locus_tag="VGR29_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV2074071.1"
                     /translation="MSIDTNVGEQIATITINNPDRLNALDADHLEGLLDAFRATAGDA
                     GVRAIILTGAGEKAFVAGANIKEMSTMSQAEAMRFGRLGHAVGSAIERTPQPVIAAIN
                     GFALGGGCELALACDIRICSTNAVFAQPEVGLGIPPGWGGTQRLPRIVGKGIASELIF
                     TGRRIRADEALRIGLVNAVHEQDSLMEHARVLAREIAASSPAAVRLSKRLIARSQDAH
                     PSSGLAEEAHGFAETFGMHDQQEGMSAFIEKRKPVFENEPHYEFEGDE"
     gene            11531..12289
                     /locus_tag="VGR29_10575"
     CDS             11531..12289
                     /locus_tag="VGR29_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEV2074072.1"
                     /translation="MKIAVLVKQVVDVGSVRIESATGEPKIGGSPVMNTSDAHAVSEA
                     IDLKETTAGEVVAISVGPRIAREELVEALATGADSAIHVVCEEMDRADSLSTARAIAE
                     AVREEGFDVLFTGQKADDFGTGQVGLQLAEMLGIRHLSNVTTITTHADFIHVARDADG
                     FPEELDVQTPVLLILAPRDDASKRHSSLRGMMQAKKKQVREVEPAVPIDSALTWTGPM
                     AQRVSADRVLLEGEPVDEAVAKLAAWLREHRLVG"
     gene            12310..13284
                     /locus_tag="VGR29_10580"
     CDS             12310..13284
                     /locus_tag="VGR29_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEV2074073.1"
                     /translation="MAPGNRVLVLAEVMGGVIIPHSHEMFAAGKAIAEGLQVPLVLGL
                     VGTDQDEALANIGNLDGSIETMTAVVDEGETFPYEAWVDATSELVRSVDPRVVLASGT
                     SFASDYAPRMAARLGLPMASWVTSVSVDHQGLVVTRDVLGGRAETAIRCDTGTPFVIS
                     LEPGMIDPSPIGDVAAISLSDVFAELAPASPGFQSTASRKRLTGADRIVSGGRGMRSE
                     EGVALIEELADVLDAAVGSSGAAVLMGLSPHETQVGSSGEVVRPKLYLAVGLSGTPQH
                     TFGMRESQFIVAINKDPSAPIFQLADFGIVGDLFEVVPALTEALKKPA"
     gene            13290..14432
                     /locus_tag="VGR29_10585"
     CDS             13290..14432
                     /locus_tag="VGR29_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074074.1"
                     /translation="MPYPGSRAFDVLLVGSGLGAAACTFELARGGARVGFVAGVGRPR
                     SLEADGGVVDPLLVLRAFGEGAPLGALVTQRQTFMAESVNTAPLLSPVEEMLPRHVYR
                     RLELETWARERAVAAGAEYVEGFIEETVLPAPGELLELISEVGDQTLSAKTIALCEGS
                     DPRISMRARLRPDYDPEDQLHFAKTIIERPAGDAVYLTGNTRTSWGMPIGVTAVPLES
                     GLIVSVVARIENIMRSNRSSTNALEDLLSSSLGFDLGLQGERIDTGVELSALRRYTRN
                     VRLSHDRLLIGLDASGMLDPREAQRGDVTIRSGTHLAEFLMDPGASPSWDEFARPLLQ
                     QVRAVNADWVDARSTGFVEEPRTGAGRTRALSRAGTGLLRRLRTRG"
     gene            14454..14849
                     /locus_tag="VGR29_10590"
     CDS             14454..14849
                     /locus_tag="VGR29_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2074075.1"
                     /translation="MNIHRLHHTSIPMPPGGNDDARRFYAGVLGLREIPPPSSLRKDR
                     LVWFSVSDDGDEIHLLTVDDFQPNTNGQHLCLVVDDLAAARQALESAGLQVGEEPEIA
                     FRPRFSFQDPFGNKIEITEINGDYLDAER"
     gene            14862..16439
                     /locus_tag="VGR29_10595"
     CDS             14862..16439
                     /locus_tag="VGR29_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2074076.1"
                     /translation="MAAIEPKITYTSTPEQIEAMQARFDDALVEVEKEFGTVYPQIIN
                     GEDRTSQQLFDVVAPARTSLVLGRFPKGTRKDVDDAIATAKAFQPKWDATPWEERVRL
                     VKAAADKVRERKFKLAAIMIRECGKSRTEAIGEVEEGADLLEYYATVFVENDAYQKKM
                     GSEDPRERNTSVLRPVGVWGIMSPFNFPNALAAGPIGAALVTGNTVVFKPASATAISG
                     YELTRAILDGGIPAGALQFVTGGGAEVGDYLVHHPQTDGVVFTGSKETGMDLHNSFST
                     TYPKPIITEMGGKNPTIVTKNADLDKAVEGVARSAFGFSGQKCSACSRAYVDASIYDA
                     FMDLLVKRTGELKVGDPTDRDVFIGPVIDESAVERYEDAVEIAKRDGGTVRYGGERLT
                     GEGFEKGTFVEPTIVDNLPLDHELFKRELFLPFIAVAPVDSLEQALKEANDVEYGLTA
                     GIFSEDKEEVGTFFDKIEAGVVYANRKGGATTGAWPGSQSFTGWKGSGSSGKGALGEY
                     YPALFMREQSRTVVIDD"
     gene            complement(16642..17808)
                     /locus_tag="VGR29_10600"
     CDS             complement(16642..17808)
                     /locus_tag="VGR29_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2074077.1"
                     /translation="MRYETFTLERWMTRWELDVDYDIAESGILPMSLADLYELITPAA
                     RERLERDLHEMPLGYSEARGTIALRIALAATYERATPEDILVTTGAIEANFLLFNALL
                     EPGDHTVVVSPAYQQLHSVPRALGAEVDLWSVVQEDGFAYDLDRFESLVRDDTRLIVI
                     NTPHNPTGAMLTAGELQDLVRIADRVGAWILSDEAYRWLDHPGGEGLPVPLHDSYERA
                     ISVGTLSKPFGLPGLRVGWFAANAEIAAKAWSVRDYVSLSPAKMSDAIAKVVIEERDR
                     ILPRNAAIIEKNLEVATTWFANNDDLVHWSPPRAGLLAMMRYDVDIPSLELADRLAED
                     ARVMLAPGSTFGIEHHLRIGIGQRPDLFEAGLDRTSDYLRRLKQFFYNQLHDGC"
     gene            complement(17805..19208)
                     /locus_tag="VGR29_10605"
     CDS             complement(17805..19208)
                     /locus_tag="VGR29_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074078.1"
                     /translation="MSSGLVRWRPQSLTGKEVAQVHSILGVAMQRLADALLQPIALLA
                     AVVWLLDGSFGDVAVITVVASASLALGSIVMPYVLGLVEDVRLVILGASTVRAAAAAL
                     IGILGWRVTSLEQSDFVALLIVSVLFYQIGSAVNVVTNPRSTIISLEQPTTARSRQVA
                     GAVAGIIGGLVAWRTLGNDTLTFPRSAGWLLALAGIASISAIWFQITAPVRRGAFRHK
                     PSLVSREEVQALLQTGTMCRFLGFRLLFGFADLADPFLVIYGIVQMGLGLRYVGAIIL
                     ILTLAQVAGGVIWTVFRQAEGTRRSLQIAAMLRLAGITIAAGVPLIANSGAYDQRFGS
                     PAIGSWAFVTAFILLGLGQSTYLRNEQAYARRIAEEESLHPASIMLVNFSHIITSMAA
                     FLGAWIIDISSLESAIVVAAMISFLALLSSGLLVGRRTPRRRVLSPGLRGPRRPSRVR
                     KRRRFRRRKPQWQGEHT"
     gene            complement(19307..20122)
                     /locus_tag="VGR29_10610"
     CDS             complement(19307..20122)
                     /locus_tag="VGR29_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEV2074079.1"
                     /translation="MRYINGDAREDGCIFCERLEAANDVESLILFRGQHTFTIMNLYP
                     YNTGHIMLVPNAHASDPAQLSMEALHEMGETLPVLTTALRTVLNCSGFNVGLNIGAVA
                     GAGVAGHLHQHIVPRWLGDANFMPILASTMVIPETIPVTYAKIRAEIQREMSSVDATS
                     FVLIDRVRSLVLLRQGALPRVDLVADQPVWQSIIEAVPREIKWFELAGWAGADVAAPI
                     DDSSIVLTLRGEIGPDLSTGWEAFRLDSPVLDAGTRSVLERAQAQIAPGSSTG"
     gene            20272..21822
                     /locus_tag="VGR29_10615"
     CDS             20272..21822
                     /locus_tag="VGR29_10615"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV2074080.1"
                     /translation="MMPTNSVKSATPSMTAESHIAMIARPMVKPSVRGRGSLQLALIM
                     QAVAMVGDIMSVFLAFWLGYALRYRLELGGEVTEDMWSSFSTFVAPAFAATTIMLVVF
                     PLRGVYQIRQKLSVLDYVPLLVGGYSLVIAGVIILAFFFQFSPSRLIFLYVGIFGLGF
                     MFAHRLVAAFVRRWFLQRGIGVDRVLIVGEGKGGRRLMQAMLGNRGLGYRLVGYVGDA
                     SRGDRLNVATERGILTCPRLGGLDDVGELVVRLHVDEVVVVDDGKGDIDIKRVLDECR
                     NTDAQFRIVPDLLQISLDRVDFSEIGGVPTIGVRDASIRGWHAVIKRTADIIVALMVC
                     VIAMIPMAIVAVLIKRDSEGPIFYRQSRVGQYGQHFTMIKFRCMVNNADQQWAEMVKS
                     MQGGDGRLFKDPNDPRMTKVGKTLRRYSIDELPQIFNVLRGEMSVVGPRPPLQREVDQ
                     YDEWHMQRLLVRPGLTGLWQVNGRSKLAFDEMVRLDLYYAENWTPWLDVKIVLRTIPA
                     VLLGRGAY"
     gene            21827..22450
                     /locus_tag="VGR29_10620"
     CDS             21827..22450
                     /locus_tag="VGR29_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074081.1"
                     /translation="MDLWSYLRALLRWWWWLVLVPLIAFVLAFFVLFPEAPWQTTWKT
                     VITFEGNPAKASSFEYVDFIVLDDMEHLLQSDVIGDQVYLHLPDEITSRYSREDIGQM
                     YSSYRHARFVQIWVTGDEPEVVEAVAQATEAVLPEAVNEYLIPPDNPDFPGMVETVDR
                     LGEPVQLAMERWQKVAAVTGVGVITALCLVGIVEWLSMSHRLKYGER"
     gene            complement(22423..23331)
                     /gene="hisI"
                     /locus_tag="VGR29_10625"
     CDS             complement(22423..23331)
                     /gene="hisI"
                     /locus_tag="VGR29_10625"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEV2074082.1"
                     /translation="MSSDLPIKWPEDGLIPAVIEDVTSGHVLMVGFMNETALTRTRET
                     GLVHFWSRSREKLWKKGETSGHVQHVENIYVNCEQNSLLIEVQQTGAVCHDGYPTCYY
                     RRLDADNSLQRVRERWFDPLDVYGTHNGVADLTRRWWGGYEFLREQDLESQSGTSRLL
                     RNSGTSVLERIQDELRELAGTIDGSHGHTNQRDDIVLEASQVSYWTAVEAIRSGFTWN
                     DVRPDRAFDRSNSESLPARSTVAKLLRVTAEGLRSDAFTPSTAHELFALIATTCLTLA
                     VDPLAPILKDLEDLQTRDYLSPYFSR"
BASE COUNT         4817 a         6441 c         7431 g         4803 t
ORIGIN      
        1 tggaaccggg aagaccgttc gaaaaccgtg tattacgtcg aagccgaaga gcagggggat
       61 cttgagtcgc gtttcctcca tcgcgatgcg ctgaagccgg ttggtttcag cgggatcggt
      121 gacgaagaga agtgaaccga cctcgccacg cctgagcgca gcttctatga cagctggttg
      181 gggtagggga cccgagccat cgggtgactc ctggtcggac acagagccaa agtagaagta
      241 ctgggtgagc tgacccgcct tttccgctgg agtcatctgc tgaaggaggc cctccgctcg
      301 ttggcgaacc ttcatggcat tgtcatttgt gggtgggtga ctggtcattg aggtggtgtc
      361 tccctggcga gtggtgatag gtgagcttgt taatgccacg gacaatcaag gtagaggcgg
      421 cgtgtcggtg ggcgagagtc cgtgaggttc ttgccggagg tgcgcgtgta ccagaccggt
      481 aatcgcggaa aagcccggat gcaatccgtc cgtcgtgtcg acggtaatag ccggatatcc
      541 cagcgtcgac agattgtagg catctctatc cagcccggct atcaagtccg ggagcgcttc
      601 caggtcgaag tgcgcgccat gccgctgacc gttcaaggag cgctcgcgat atcgacgaac
      661 gattgtctcg cgatccgacc aacattgaac gatctgaacg cgcgcggttg ttgacatccg
      721 attgaggtcg gccgaagcga acgcgcgatc gaaattgcat tccaccatga ggctcgtgcc
      781 atgtcggagc atcggcgtgg caagggcgta gagcaaccgc gtgctggcgt aatccaattt
      841 tcgagagtct tccccgtctt gtgttccgat cgcgtccgcc aacgactcct tgatgacgtc
      901 cttctccagc agaggtaatc gcaggtttcc ggccaggcgc cgggcaaggg tcgtcttgcc
      961 tgaggccggt gcgccacaaa cgacgatgag ggcgggcaga ggaacatctt ccatcatgct
     1021 ggaaactcat cacacttcag cgggacacca ccttatccgc ttcgtcacgt gcaggagcaa
     1081 tagcgagccg tgaaggcacg gcgtcgggcc gtacctggaa gttgaatttg gtccggatgg
     1141 cgtcgaagaa gttcgtgatg gtgacgcgga tcaactggaa ggtgtgttcc ccacgttgaa
     1201 tcttgatgag atcgcccttt tggacttcca tagtctgttg gccatcgagg atcatgtatc
     1261 cggggtggac gttagagtgg atgatctcga tgagcgcctc ttccgaggtg atcattggtg
     1321 tgcggatggg ggaatggcag ttgattggca cgaccgcgaa accacgcacc ccgggtgtga
     1381 ggatcggacc gccggctgcc atgcagtagg cggtgctacc gcgcggggtg gagacaatca
     1441 tgccatcccc cggataggtg ttgacgtagt atccgtcgat atagacttcg tcctcgatca
     1501 tccggttgcc accgcggata accacgtcgt tgatggccca gccctggtca tactccttgt
     1561 catcccggta gatcgaagcc tggagcgtcg agctttcgtc gatatcgtat tttccgtcca
     1621 ggaagagttg gagtgcggct gtccagtcct tctgctccac gagcgccagg aaaccaacct
     1681 tgccaaagtt gatgccgaac aagggaatgt ccggaaaggc gttggccatg cgcatcatca
     1741 ggccgtcacc gccgaagacg atgattgcat ccacatttcg ccggtcgccg gcaaggatgt
     1801 ctggctcggc tacggtcgtg tgtccccgag cctcaagcca ttgcctgact gttgtactca
     1861 gctcctttgc ttcgggttta ctgaacgcgg cgacgatacc gagccgttgg gacatccggt
     1921 ggttctccct caatacatgc ggcacagcgc attagtcctc gtgtcttgga ggagtcagat
     1981 aggaagcctg gtcatcattc aggttgaagc gataaagagc cgccggacgg tgactgcctt
     2041 cgcgacgctt ttgacgagta tccgtgagaa tgccgctggt aatcattcgg cggcggaagt
     2101 tgcgcttgtc cattggttgg ccaaggatcg tctcgtacgt ctgctgcaat tcggatagcg
     2161 tgaatgtgga cggtacaaga tagaacgcga tgttcgaata tccgagcttg gcgcggagac
     2221 gaatgagtgc ataatcgagg accatctgat cgagaccatc tgcaggttgg gcatccttga
     2281 ccagaggcca ggcgagatca atggtgccgg ctgtaacagg cgtatctgga gagaagaggc
     2341 cgagatagct gatcacgatc tccctcaccg gtccatgacc ggggctgaag gtataaagct
     2401 gctcaaggta ctgtgcatcg accccggtcg cctcgcgaat gatgcgtacc gctgtctggt
     2461 ccaatgtttc ctccgcagtc ggaaccgaag aaggaagaga gccggcatga cagattgctg
     2521 tttcaagctg ggtaccattc caccggatga gtgcaacaca gacacggaaa gatattcctc
     2581 gtgttgcgtg cggggtatcg tcggaggtta actgggcgga tggcatgtgc ggctggatca
     2641 ttgatgggtg ggccttgtcg ccgatcacgt atgccggcgg gatggagaga agctccggga
     2701 gaggcggcgc tcaacctgct gcaagaatgc cagaagtcgc acctgtctgg cacgtccagc
     2761 cgtttcccgg tagacttggc gtggccgctt tggcggatgg tgaaatggta tcacaagggc
     2821 ctttggagcc tttattccag gttcgaatcc tggtccgcca gccgaaatgc agcccatgtt
     2881 ccccgtcgca tcgcacaacg gaaatgatgt tccccaagtg accaacgaaa ccgctccttc
     2941 cggtgtcgct ccgcgcgtgg cagtggcggt cctcgccgct ggacatggaa agcggatgcg
     3001 gtcgcagact ccaaagcata ttcatcctgt cggaggagta ccgctggttg agcgaattat
     3061 tcgcgccggt ctctccatcc agccggcacg cttgctgacg gttgtcagtc ctgccatggc
     3121 tggcatgccg gcgatgctcg gcatgcaggg agaattcgag accgtgattc aggatcctcc
     3181 ccgcggcacg gcggatgctg tgcggatcgc tctatgcgaa gctcccgagg ccgactacct
     3241 cgtttcattg ctgggcgaca acccgctctt gactgcggag atcgtccaga cactggtgga
     3301 tggtgctatc acatcagaca cgaagatcac catcctgacg tgcctcctac cagacgccca
     3361 agcatatggc cggattcatc gagacgatct aggcagggta acaagcatca ttgaggcgaa
     3421 gaatgatcat ccttcccagc gcatcggtga gacggagatc aatagcggca tcatggtatt
     3481 ggatgccgcc tgggcgcggg aggcattgca gcagctccct ttggatccgg tgactggcga
     3541 gtatctcctt actgacctgg tggagatggc tgccagcgca catgtcgagg gagagccgtg
     3601 gcccatcgct acggtccagg ctccggtgac cgtctccctg ggtgtcaaca atcgcctgca
     3661 gcaagctgaa gctgacgcat tggtccggcg ccaggtccgt gagcgtctca tgcataccgg
     3721 tgtctcaatg atcggtgccg acacgatctt tatcgacgag caggtgcaga ttggtatgga
     3781 caccgtgatc ctgccaggca cgatcatcat gggctcgacg acgattggct gcggctgccg
     3841 catcgggccc aacactgtct tggatcatgc gacgatcggc gacaatgtcg tgatccagtc
     3901 ttcgacggtc gagtattcaa tgatggagag tggctccgac gccggtccat acgcgcatat
     3961 tcgtggtgga tccgtcatcg gggaagaggt tcacgtcggt aattacgccg aactcaagaa
     4021 cactgcaatg gcccgcggcg cgaaagtggg acatttctct tacctggggg acgcgacggt
     4081 cggtgagagc gtcaatattg gtgctggttc agttacggct aattatgacg gcaccacaaa
     4141 aaaccggaca gtcctggaga ggaatgtgtt cgtcgggtcc gacacggtct tcgtcgcgcc
     4201 ggtgaccgtt ggagaggggg cgaagaccgg cgcgggcagc gtggtgactc gcaatgtccc
     4261 gccgaacacg acggtcgttg gtgtgcccgc gcgtcagatc gccaaacggt cggccgagaa
     4321 gagcgatggc caagagcagg actccggccg tacgtacaag gagtagcatg gacggtcgtc
     4381 ttcaggtctt cgcagggaat gctcatccgg cactcgcaag agcgatctgc gaggaactcg
     4441 aagtcgatct tggccgtgcc attgtgacgc aatggaagaa cggcgagacg cgggtcaagc
     4501 tggaggaaaa cgtccgggga tctgacgtct tcgtagtgca accgacttct tgcccggtta
     4561 accaccactt gatggaactg ctcctgatga tcgatgcctt gcgtcgtgca tcggcgaccc
     4621 ggattacagc ggtcatcccg tactacggct acgccaagca ggagaaaaag acctcggggc
     4681 gcgagccaat ctctgccaag ttggtcgcga acctgctgac cgttgcaggt gcgacccgtg
     4741 tgcttgctgt ggatatgcat tcaccggcga tcgagggatt cttcgatatc cccgtggatc
     4801 atctacgagc aacaccgctg ctggctcgtg cactccggca cgatattcag catgacatcg
     4861 tcgtggtgag tccggatgcc gggggtgttg tgcgtgcgga ggatttccga gagcgggcga
     4921 agggttccct ggccatcatc tccaagcagc atccgggcgc ggataagacc gagaccgtgg
     4981 agatggttgg cgatgtggat ggcaagattg ccgttatcgt tgacgacatg atttccaccg
     5041 gtgggacctt ggtgcaagcg gcgaagttgc tcaaggagcg tggtgccgcg aaaatccacg
     5101 tctgcgcgac tcatggcgtc ttcgcagacg acgctatcca gatgatcgac gcatcgtcga
     5161 tcgacgagat catcgtgacc gacacgattc ccctacctga gcacggtaag ggcccggtga
     5221 aggtcgtcag cgtggcaccg ttgctcgcgg aagcgattat gcgtacccac aagggcttga
     5281 gcgtgagcgc gctcttcacc tagatcaggc gctctttcat ctcttgtcgg gcctctcgag
     5341 cgcaaggcaa cctggcgcgg ctttggccag cctcattctt atcttcgatt cataatcaat
     5401 ccggcttgag ccggagcagg cacaggggta aattttcttg gacgccgacg attggcaaca
     5461 tgtgatattc gcgatcctcg cgctgctgtt agtagccgct gcgggagtcg tcttgacgat
     5521 cgtcgggcag gcgacaaatc aacggctcct cccagagact gctgatcctc tccaccgggg
     5581 aaagcgctca acccgttccc tggtgaatcc gcgcagggcg acggccagcg cgatgatctt
     5641 cgtacagatt ctggcgacgg tcatcgcggc aagtcagatt actcagctta ttgaccagtg
     5701 gacgcacgat gatttcaatt ggacggccat tttggtcgtt gcgatcgtct atatcgtctt
     5761 tggacagtcg cttccccgcg cgattgttga caagcaatta accgaccagt tcttgagccg
     5821 cgtgctcgtg gtcggccgcg ctgttgcaca gctactggca ccggtatcat ggctggtcgt
     5881 gggtcttgcc aacctcttca ggagggtcct tccgggcagg gacgccgaag tcgaagcgat
     5941 tggcaccgag gatgaattga gccggagccg gttgtctccc gacgatggtg tcattggcga
     6001 aaatgaacgc cagatgatcg acggcatcct gagtctggag gagatgacgg ttcgggacat
     6061 catggtgccg cgccttgaca tcgtcggctt ggagcggacc gtgagtgccc gcgagctgat
     6121 cgagacgatc acgaaggcag gacattcacg tattcctgtc taccgcgaca ccatcgataa
     6181 cgtgctgggc gtcctctacg tcaaggatct tttaccgttt gtcatcggga ataccattcg
     6241 catcccgcta ctagatctga tccggccggc gtttgtcgtg cccgagtcta agcggctcgg
     6301 ggagcttttc acggaattga aacgcacccg tatccatctc gctgtggtag ccgacgaata
     6361 tggcgggacg gcgggcctcg ttacgatcga ggacattctg gaggagatcg taggcgagat
     6421 ccaggatgag tacgataccg agtcgccgct tttcgagcag ggcgaagacg gatttatcgt
     6481 cgccgacggc cgtctcccca tcgaggacgt tgaggatgcg ctcggggtcg agttcgagga
     6541 agatgacgac ttcggaaccc tcggtggctt tgttcacaag catctcgggc gcctgcccat
     6601 ccagggcgac acgttcgaag ccgagaacgt gcgggtcgag atccttgaag tcgagcggca
     6661 tcgggtccgc aagatgcgcg taacgcggct ggaagtcgaa gacgaagagc ccgcagacga
     6721 ggtgcgagaa cggtccgacc gttggcgacg gggcggaggt agcgacacct acgacgagaa
     6781 cgcgacgtca gtggaggaca ggctgccttg atgcttgggc gcccggtcat ccggtttgat
     6841 gcggtcgcca gcacaatgga tgtgcttgcc gatctggcgg cactcggcgc ccctgaaggc
     6901 acgatcgtta ttgctgacta ccagagtgca ggacgcggac gatccggccg agagtggaca
     6961 gcgccgccca gaacttcgct gctgtattcg atcctgctgc gaccggagct gccgatccgg
     7021 cagttgaccc ccctctccct tctggttgcc gatgcgattg tcgccactct gggagagtgt
     7081 cacggtgtcc aggcagagat caagtggccg aacgatgtcc tcatcaaggg caaaaaagtt
     7141 tccggcgtac tcgcccaggt gaagtcccaa gtggacggga aggtcactat cctgggcgtt
     7201 ggggtcaatg caaatatcct gacggccgat ctcccctcca atgcgaccag tcttttggtg
     7261 gagctgggcg agcctgtcga tcacaatgca ttgatgtggc gcttcctgaa tgagctggag
     7321 agccgatacc aggatctact gtccagtcgc atcgaacaac gctggttagc gatcaggaat
     7381 acgctggcga tgcttggtga ttccgtgacg gttaaggagc ggcatgcgga catccatggc
     7441 atcctgcagg gcattgacga cgaaggggcc ctgctgctcg atcagggtgg cgtcatccgg
     7501 cgcgttgtgg cgggcgattt gactcgggga ccgagacctg atgaacctcc ggatgcagaa
     7561 acggcaactt cgctaccccg tatacttcca cgcggttgat gctgattccc tgaggggtcc
     7621 aacgaaagtg gttgctgcag aatgaatttc gagcttactg aggagcaggt ccaggtacgt
     7681 gacatggtcc gtgagtttgc ggagcgcgag gctgcgccac acgtccggga gtgggacgca
     7741 aaacagaact acagccggga tatcatcgac aagatggggg aatcgggaat actgggtttg
     7801 ccgattcccg aggagtatgg agggctcggg ctggattacg tcagcctggc actggcctgc
     7861 gaagagtttg agtacgtcga tacatccttg cgagtcgtac tcagcgtgca tatcgggctc
     7921 aacagcctgt ccttgctgca gtgggcgaac gacgaacaga agcagcgatt tcttgttcca
     7981 caggccactg gggagaagat tgctaccttc ggactcacgg aacccggcgc ggggtccgac
     8041 gctggtgcta tcgagacgac ggcgaccagg gatggtgacg attacattct caatggttcc
     8101 aagatgtgga tctcactttc ggacatcgcg gacaattttc tcgtttttgc cagcctggac
     8161 cgttcacaga agcacaagag cttggtcgcg ttcgtggtcg agcgcggcat ggagggcttc
     8221 accaccggga caatcaaggg aaagctcggt gtgcgggcag gcaacaccgg tgagatggcg
     8281 ttcgacaacg ttcgggttcc cggcgccaac cgtattggcg aagaaggcga aggcttcaag
     8341 atcgccatga gtgcgattga ccagggacgt ttcacggttg gagcaggggc tgttggcctc
     8401 acgcgggcag cactggatgc aagcgtcagg tatgcgaatg accgccaaac gtttggagtg
     8461 ccgattggca agcatcagct cgtccagcag atgattgcaa agatggtcgc cggatacgag
     8521 gcatcacagt tgctggtcta caaggccgcc gagctcaaga accggggtat tcggaacacg
     8581 cgggagacat ccctcgcgaa gtggtatgcc actgaggcgg cgctgcaggc agccgatgac
     8641 gcagtgcaga tccacggatc ctacggcttc gccaacgact acccggtcga gcgctacctc
     8701 cgcaacgcgc gcggatcgac gatttacgaa ggcacgagcc aaattcacca gatcttgcag
     8761 gcggaatacg ccttgggcta ccggattgac aaaccgctga gccgtccgca gccgccggcg
     8821 gtcggcttcg aggacgaagt ttaatcgtgg cttgtctagc gcgaatggac gttacgtcag
     8881 gccgggcata catgtgcccg gcctatttcc tggcgggatg accgataatg gaatgagcag
     8941 atattggcgt gtgcgaggag aaaccagtgg gaagatcggt cattcttggc ggggcgcgaa
     9001 caccgtttgg aaaactcaac ggagcattgg cgtccctcac tgcagtcgag cttggcaagg
     9061 tggcggcctg ggcagcaatc gatcgcgctg ggatcgaaga tgatgcgatc aaccacgtca
     9121 tcatggggca ggtgcttcag ggaggtgcgg ggcagaaccc agcccgccag gttggactgg
     9181 gcctggggct cgaccgaaca gtcactgcgg agactatcaa ccgggtttgc ggttcaggtt
     9241 tgagatcggt tgcgctggcc gacatgcaga tccgcctcgg tgaaagcgcg gttgtgcttg
     9301 cgggcggcat ggaaagcatg tcaaacgcac catacctgct ccgtggcgca cgggccggct
     9361 atcgcatggg cgacggcgag cttgtggaca tgatgatgca cgatgggctg aggtgcgcga
     9421 ttgaaggctg ttccatgggc ttccatggtg gccgtgtctc tgacgaggag cgaatctccc
     9481 gggaggagca agacgcgtgg gcgctccggt ctcatcagcg agcgcttgca gcgattgacg
     9541 atggccggat ggcagaggag atcgttccgg tcgaggtggc ccaaaagaaa gggacaacca
     9601 tcgtcgatac cgacgaatcg cctcggcggg agacgagcgc cgaggcactg gcgaagctca
     9661 agcctgcgtt ccaaaaggac ggcaccgtca cggcgggcaa cgcgcccggt ataaacgacg
     9721 gcgctggagc agttgtcgtt gcagatgaga tctgggccag gcagcgagga ctcacgccga
     9781 gagccacgat cctcgccacc gggcaatctg cgtgggatgc gccgtatctc gcttacacgc
     9841 ctgaaatggc gattcggaat gcccttatga agactgagca cacgctggag gatatcgagc
     9901 tcttcgagat caacgaggcc ttcgccagcg tcgcgatcat tgccagtaag cgtcttggca
     9961 tcgatccaga caagctgaac gtcactggcg gcgcagtcgc cctcggtcac cccatcgggg
    10021 caagcgggac gcgactcgtc atcaccgtga tcaatgagct gacacgtcgc ggcggcggtc
    10081 ttggcgcggt agccatttgc tccggtggag ggcagggtga cgccatgatc ctgaaggtgg
    10141 aggcgtgatg gatcggcgcg ccgagttctc ggcgagattt cccggcgcga tcatcctgcc
    10201 atttgaagac gcgtggccag ccatcgccga cgacgccttg atcgcacccg gcgctgtgct
    10261 gatagggagc gtgaccattg ggtccgagtc cagtgtctgg ttcaacacga ctatccgcgg
    10321 agatatcgcg ccagtcacaa tcggagcccg gacaagtgtc caggataata cggtcgtaca
    10381 cgtcaacatg gacgccgcta cgactatcgg ggatgacgtc acaatcggtc acagtgccct
    10441 tgtgcatgga acgacgattc acaatggagc actgatcggg atggggtcac tcgtcctctc
    10501 ctattcggtg atcggccgga acgctgtagt ggccgccggc gcgctcattc ccgagcggtt
    10561 ctatgtcccg gatggtgcag tcatggtcgg cgttccggcg aagcaacgtg acgccctgag
    10621 cgacgagcaa gccgagcgac tcgatggtat ctctggtcgg tatgtgaggg tgagtcgcac
    10681 ctaccgcgag accgtgatga tgcttccgag cggggaagag gaggagcctg aaggtgagca
    10741 ttgacaccaa cgtgggcgag cagatcgcta ctatcaccat caataacccc gaccgattga
    10801 atgcgcttga tgccgatcac ctcgaggggc tgctggatgc cttccgggcc actgccggtg
    10861 acgccggcgt gcgggccatc atcctcactg gagctggcga gaaggcgttt gtggcaggcg
    10921 caaacatcaa ggaaatgtcc acgatgtccc aggcggaagc gatgcgcttc ggaaggctgg
    10981 gtcacgcggt gggatcggcc atcgagcgca ctccgcagcc ggtgatcgcg gccatcaatg
    11041 gcttcgcgct tggcggtggt tgcgagctgg ccctggcctg tgacatccgc atttgctcga
    11101 cgaacgcagt ttttgcgcag cccgaagtcg gtttgggtat tccaccagga tggggcggga
    11161 cacagcggct gccgcgcatc gtcggtaaag gtatcgcctc ggagctcatc ttcaccgggc
    11221 ggcgtatcag ggccgacgag gcgctaagga tcggcctcgt caacgcggtt catgagcagg
    11281 acagcctgat ggagcatgct cgcgtgctgg cccgggagat cgccgcgagc agtccggcgg
    11341 cggttcggct ctcgaaacgg ctgattgccc gttcccagga tgcacacccg agctcggggc
    11401 ttgccgagga ggctcatggc tttgcggaaa ccttcgggat gcacgaccaa caagagggca
    11461 tgagcgcatt cattgagaag cgcaagccgg tgtttgagaa cgaaccccat tacgaattcg
    11521 agggagacga atgaagatcg cggtccttgt caagcaggtc gtcgatgtcg gatctgtacg
    11581 catcgagagc gcgaccggtg agccgaaaat tggtggttcc ccggtcatga atacttcaga
    11641 tgcacatgct gtttccgaag cgattgatct gaaggagacg accgccggcg aggtcgtggc
    11701 aatcagtgtg ggaccacgga tcgcgcgcga ggaacttgtt gaggcgctgg cgaccggtgc
    11761 ggatagcgca attcacgttg tttgcgagga gatggacagg gctgattcct tgtcgacggc
    11821 gcgggcaatc gcggaagcag tgcgtgagga agggttcgat gttctcttca cgggccagaa
    11881 ggcggacgat ttcggtacgg gtcaggtggg tcttcagtta gcggaaatgc tgggaattcg
    11941 ccatctctcc aatgtgacca ccataacgac ccacgcagat tttattcatg tcgcgcgtga
    12001 tgcggacggc tttccggaag agctcgacgt ccagacgccg gtcttgctga tcctggcgcc
    12061 gcgtgacgat gcgtcaaagc ggcattcctc cttgcgggga atgatgcagg cgaagaagaa
    12121 gcaagtacgt gaggtggagc cagcggtccc tatcgacagt gcgcttacat ggacagggcc
    12181 gatggcccag cgcgtgagtg cggacagggt tcttctcgag ggtgaacccg tcgacgaggc
    12241 cgtggcgaag ctcgctgcgt ggctgcggga acatcgactc gtcgggtagt tgcggaagga
    12301 cccaggaaaa tggctccagg aaatcgcgtt ttggttctgg cggaagtcat gggaggggtg
    12361 atcattccgc actcgcatga gatgttcgct gctggcaagg ctatcgctga ggggcttcag
    12421 gtgccgcttg tccttgggct ggtcgggacg gatcaggacg aggcactcgc gaacatcggg
    12481 aatcttgacg ggtcgatcga gaccatgacc gcggttgtgg acgagggcga gacgttcccg
    12541 tacgaggcat gggtcgatgc cacaagcgaa ttagtaagga gcgtagaccc acgtgtcgtc
    12601 ctcgcttcag gaacgtcgtt tgccagcgac tatgctccac gtatggcggc tcgtcttgga
    12661 ttgccaatgg catcatgggt cacctccgtc agcgtcgacc atcagggtct ggttgtcacg
    12721 cgggacgttc tgggtgggcg cgcggaaacc gcgatccggt gtgacaccgg gacccccttt
    12781 gttatttcac ttgagccggg gatgatcgat ccctcaccga taggtgacgt ggctgctatc
    12841 tccctttcgg acgtctttgc cgaattggct ccagcgtctc ccgggtttca atcaacagca
    12901 tctcgcaagc gactgacggg tgcggatcgg atcgtctcgg gtggccgggg catgcggtcc
    12961 gaggagggcg tcgcgctcat tgaggaactc gccgatgtgc tcgatgcggc cgttgggtca
    13021 tccggcgccg ccgtgctcat gggattgagc ccccacgaga cgcaggtggg atcgtccggt
    13081 gaggtcgtga ggcccaaact ttacctagca gtcggactca gcggtactcc ccaacacacc
    13141 ttcggcatgc gcgagagcca gttcatcgtg gccatcaaca aggatccgag tgcgcccatc
    13201 ttccaacttg cggattttgg tatcgtcggt gatctcttcg aggtcgtacc cgcgctgacc
    13261 gaggcgctga agaaaccggc atagtcgaga tgccatatcc aggcagccga gctttcgacg
    13321 ttctccttgt agggtccggt ctcggcgcgg ctgcctgtac ctttgaactc gcgcgaggag
    13381 gggcgcgagt agggtttgtt gccggcgtag ggcgtccgcg gtcgctagaa gccgatggcg
    13441 gggtcgtcga cccactgctg gtgcttcgtg cattcggaga gggtgctccc cttggtgcgc
    13501 tggtgacgca gcgacagacc ttcatggcgg aaagtgtcaa cactgctcct ctcttgagtc
    13561 ctgtagagga gatgctcccc cgccacgttt accgacgcct tgagcttgag acctgggcac
    13621 gcgaaagggc agttgccgca ggtgccgagt atgtcgaggg gttcatcgag gaaacggtgc
    13681 ttccggcgcc aggtgaactc ctggagctca tcagcgaggt aggtgatcaa acgttatccg
    13741 ccaagacgat tgcattatgt gagggtagcg atccccgaat ctcgatgcgt gcaagactcc
    13801 gtccggacta cgatcccgag gaccagttgc acttcgcaaa gacgatcatt gagcgacccg
    13861 caggtgacgc ggtgtacctt accggtaaca cgcgcaccag ttggggaatg ccaattggcg
    13921 tcactgccgt tccgcttgag tcgggcttga tagtctcagt ggtggcgcgc atcgagaaca
    13981 tcatgcggag taaccggtca tcgaccaatg cattggagga cctattgtcc tctagtctgg
    14041 ggtttgatct cggattgcag ggtgaacgga tcgacaccgg ggttgaactg tcagcactac
    14101 gtcgatacac acgaaacgtg cgtctctcgc acgatagatt gctaatcggc ctggatgctt
    14161 ccggcatgct tgatccacgt gaggcgcaac gtggcgacgt cacaatcagg agcgggacgc
    14221 atctggcaga attcctgatg gatccaggtg catccccttc ctgggatgag ttcgctcgac
    14281 ccttgctcca acaggttcgg gcggtgaatg ctgattgggt cgatgcccga tcgaccggct
    14341 tcgttgagga accaaggact ggagccgggc gaaccagagc gttgtcgcgt gcagggactg
    14401 gtcttcttcg aaggttgcgt acccgtggct agaccacgtc aggcaggaga atcatgaaca
    14461 tccatcgtct ccaccatacg agtatcccaa tgccgcccgg tggcaacgac gatgctcgtc
    14521 gtttctatgc gggggtattg gggctgcggg agatcccccc gccttcttca ttgcggaagg
    14581 accggctcgt ctggttttcc gtcagtgacg acggggacga aatccatttg ttaacggtcg
    14641 acgatttcca gccgaacacg aacggtcagc atttgtgcct ggttgttgac gacctggctg
    14701 cagcgcggca ggcacttgaa tccgcgggtc tccaagtcgg tgaagagccg gagattgcat
    14761 tccgaccccg tttttcgttt caggatccct ttgggaataa aatagagatt acagagatca
    14821 acggggatta tctggacgca gaaagataga ggggcaaaat catggcagcg attgaaccga
    14881 agattaccta tacatcgacg cccgaacaga ttgaagccat gcaggcaaga ttcgatgatg
    14941 cgttggtaga ggtcgagaaa gaattcggca ccgtctatcc gcagatcatc aacggggagg
    15001 atcgcaccag ccaacaactc ttcgatgtgg tggccccggc gcgaaccagt ctcgttctgg
    15061 gacgattccc aaagggcacg aggaaggatg tcgatgatgc gattgcgacg gccaaagcgt
    15121 tccagcccaa gtgggatgcc actccctggg aagagcgggt tcggctggtg aaggccgccg
    15181 ccgacaaggt gcgcgagcgc aagttcaagc tcgcggcgat tatgatccgg gagtgtggca
    15241 aatcccggac ggaggcgatc ggcgaggtcg aggagggagc ggacctactc gagtactatg
    15301 cgaccgtatt tgtcgagaac gacgcgtatc agaagaagat ggggagcgaa gatccgcgcg
    15361 agaggaacac ctcggtcctg cggcccgtcg gggtctgggg catcatgtca ccgttcaatt
    15421 tcccgaatgc acttgcggct gggcccatcg gggcggcatt ggtaactgga aacactgtcg
    15481 tcttcaagcc agcgagcgcg acggccatct cagggtacga gttaacccgg gcgattcttg
    15541 atggcggcat cccggcggga gccttgcagt tcgtcaccgg gggaggagct gaggtgggcg
    15601 attatctcgt tcaccatccg cagacggacg gcgtggtctt caccggatca aaagagaccg
    15661 gtatggatct ccacaactcc ttcagtacca cctacccgaa gccgatcatc actgagatgg
    15721 gcggcaagaa tccgacgatc gtcacaaaga atgccgatct cgacaaggcg gtggagggcg
    15781 tcgcgcggtc cgcttttggc ttctccggcc agaagtgttc tgcctgttcc cgtgcctacg
    15841 tcgatgcatc gatctacgac gcgttcatgg atctcctggt caagcgcacc ggggaactca
    15901 aggtgggcga tccgacggac cgggacgtct tcatcggtcc agtgatcgat gagtcggcgg
    15961 ttgagcgata cgaagacgcg gtggagattg cgaaacggga tggcggaacc gtccgctatg
    16021 gcggtgagcg actgacgggt gagggcttcg agaaggggac gtttgttgag cccacgatcg
    16081 tcgacaatct cccgctcgat cacgagctct ttaaacggga actcttcctg ccattcatcg
    16141 ccgtggctcc ggttgactcg ctcgaacagg cgcttaagga ggcaaacgac gtcgagtatg
    16201 gattgaccgc gggcatcttc tcggaggaca aggaagaggt tggcaccttc ttcgacaaga
    16261 tcgaggccgg cgtcgtctat gcgaatcgta agggcggtgc gacaaccggg gcatggcccg
    16321 ggagccagtc attcacgggc tggaagggta gtggctcatc tggcaagggc gcgctaggcg
    16381 aatattatcc cgcgctcttc atgcgggagc agagccggac agttgtgatc gacgactaaa
    16441 ggaggggacc gctggaaaca ataccgcttg tacggtcgga cggtctctct tgcgacgcgg
    16501 agcgatttct acagggtcta ttcagtcctc acaccgcatt gcaatagagc tgcttgcagg
    16561 atcattgccg gtttctcctt tatggcgggg aaggacgaca ttgctgcggc aagtcgtccg
    16621 ctacatggaa caagcaaagc tctagcaacc atcatgtaac tggttgtaaa agaattgctt
    16681 gagcctacgc agatagtccg atgtgcggtc caagccagcc tcgaagagat ccggacgctg
    16741 cccgattcct atgcggaggt ggtgctcaat accgaaggtc gagccgggcg ccagcatcac
    16801 tcgggcgtcc tcggccaacc gatcggcaag ttcaagcgat ggaatgtcga cgtcatagcg
    16861 catcatagcc agcaggccgg cccgcggagg agaccagtgg acgagatcgt cgttattggc
    16921 gaaccaggtc gttgccacct cgagattctt ctcgatgatg gcagcattgc gggggagaat
    16981 ccggtctcgc tcctcgatga ccactttcgc aatcgcgtcg ctcatctttg caggactgag
    17041 ggaaacgtaa tctcgaacgc tccaggcctt cgcggcaatc tcggcatttg ctgcaaacca
    17101 gccgacccga agcccgggta ggccaaaggg cttggagagg gtacccacgc tgatggcccg
    17161 ctcatagctg tcatggagcg gcacaggaag cccttctccc cccggatggt caagccagcg
    17221 atatgcttca tcagagagaa tccaggcgcc gacccggtca gcaatccgca ccaggtcctg
    17281 caattcacca gcggtaagca tggcaccggt ggggttgtgc ggcgtattga taacgatgag
    17341 ccgagtatcg tcccgcacca gggattcaaa ccggtcaagg tcgtaagcga aaccgtcctc
    17401 ctgcacgacg gaccagaggt ctacctctgc accgagggcg cgtggaacgc tgtgcaattg
    17461 ctgatatgcc ggggagacga caacggtgtg gtccccaggc tccaggagtg cgttgaagag
    17521 aagaaaattt gcctcgatgg cgcccgtggt gacaaggatg tcttccgggg tagcccgctc
    17581 ataggtggcg gccagagcga ttcggagcgc gattgtgccc cgcgcctcgc tgtaaccgag
    17641 cggcatctcg tggaggtctc gctccaggcg ctcgcgtgcg gcgggagtga tcaactcata
    17701 gagatctgcg agcgacatcg ggaggatacc gctctccgcg atatcgtagt cgacatccag
    17761 ctcccaacgt gtcatccacc gctcgagggt aaacgtctca tatctcaagt gtgttctcct
    17821 tgccactgtg gcttgcggcg cctgaagcgc cgacgcttac ggacgcgact cggtcttcgg
    17881 ggaccgcgga ggccagggga gagcacgcgc cggcgagggg ttcttcggcc gaccagaagc
    17941 ccgctggaaa gcaacgccag gaacgaaatc atggcggcga caacgatcgc gctctccagt
    18001 gacgaaatgt cgatgatcca tgcaccgagg aatgctgcca tcgaggtgat aatgtgagag
    18061 aagttcacca gcatgatcga cgccggatga agcgactctt cctcagcaat gcgcctcgcg
    18121 taagcctgct cgttacgcag gtatgtactt tggccgaggc cgaggaggat aaacgcagtc
    18181 acgaatgccc aggacccgat ggccggggat ccgaagcgtt ggtcgtacgc cccggaattt
    18241 gcgatcagcg ggactcccgc tgcgatggtt attccggcaa gccgtagcat ggcggctatc
    18301 tgcagggagc gacgtgttcc ctcagcctgc cgaaacacag tccagatgac gccaccggcg
    18361 acctgtgcca aggtgaggat gaggataatc gccccgacat agcgaaggcc caatcccatt
    18421 tgcacgatgc cgtagatgac aagaaaggga tccgcgagat cagcaaatcc aaaaaggagg
    18481 cgaaagccca ggaatcggca catcgttccg gtttgaagaa gcgcttgcac ttcctcccgg
    18541 gagacaagtg aaggcttatg tctgaatgcc ccgcgacgga cgggtgcggt gatctggaac
    18601 caaatcgcgg aaatgcttgc aatcccggcg agcgcgagga gccatccggc agagcgaggg
    18661 aacgtgagcg tgtcgtttcc aagggttcgc catgcaacga gcccgccgat gatgccagcc
    18721 accgcaccag cgacctgacg tgaccgcgcc gtggtgggct gctcgaggct gatgatcgtc
    18781 gagcgcggat ttgtcacgac attcacggca gagccgatct gatagaaaag cacgctcaca
    18841 atgagcaacg ccacaaagtc gctctgctcg aggctggtca cacgccagcc gaggatgccg
    18901 atgagggccg cagcggcggc gcgcactgta ctggctccga ggatcaccaa gcgcacgtct
    18961 tcgaccaggc cgaggacgta cggcatgacg atacttccca gtgcaagcga ggccgatgca
    19021 actacggtga tgactgcgac atctccgaag gagccgtcaa gcagccacac gaccgccgca
    19081 agcagcgcta tcggctgcaa caaggcatct gcaaggcgct gcatcgctac accgagtatt
    19141 gagtgcactt gcgcgacctc tttgccggta agtgactgtg gtcgccagcg gacaagcccg
    19201 gaactcacgg aacctcctgg agcgggaaga cgatatatcg acggggcaca cccctgaagg
    19261 gtgccgcgaa acggctccac gtcgtgccag tataggtgtt cactgatcac ccggtgctac
    19321 taccaggggc aatttgcgcc tgcgctcgct caagaactga tctcgtacct gcatcaagaa
    19381 ctggggaatc cagacggaag gcctcccatc ctgttgacag atcagggcct atttctccac
    19441 ggagcgttag cacaatcgat gagtcgtcga tgggagccgc aacatccgct cctgcccatc
    19501 cggccagctc gaaccatttt atttcacgcg ggacagcctc aatgatgctt tgccacaccg
    19561 gctgatccgc cacaagatcg acccttggta gcgcaccttg gcgcaggagt acgaggctgc
    19621 gcacccggtc aatcagtacg aacgaggttg catccacgct gctcatctcg cgttggattt
    19681 ccgcccgaat cttcgcatag gtgacaggaa tggtttccgg gatcaccatc gtgctcgcaa
    19741 gaatcggcat gaagttggcg tcccccagcc agcgtggcac aatgtgttgg tgaaggtgcc
    19801 ctgccactcc tgccccggcc acggctccaa tattcagtcc gacgttgaat cctgaacagt
    19861 tcaacaccgt ccgaagcgcg gtggtgagga ccggcagcgt ctcgcccatc tcatgcagcg
    19921 cctccatcga tagctgtgcc gggtcggaag cgtgagcatt tggcactaac atgatgtgtc
    19981 cggtgttata gggatagagg ttcataattg tgaaggtatg ctggcctcgg aaaaggatta
    20041 gggattccac gtcattggcg gcttcaagcc gttcgcagaa gatgcagccg tcttccctgg
    20101 catcaccatt gatgtagcgc atccgccaag gcgtccagag tcgttccgta ggagatgtgt
    20161 gttccaaatc cattgtgcct caatgcttct gacgtcccta gcaggaacgc tgggcgccgg
    20221 ttactatcgc aggtgcgacc gctcgtcatt gtacgtttgc atagagatct gatgatgcct
    20281 actaacagtg tgaaatccgc aactccctcc atgacagccg agtctcacat cgccatgatc
    20341 gcacggccga tggtgaagcc atcggtacgg ggtagagggt cgctccagct cgccctcatc
    20401 atgcaggcgg tcgccatggt cggcgacatt atgtcggtct ttcttgcgtt ttggctggga
    20461 tatgcgcttc ggtatcgcct ggagttggga ggtgaggtta cagaggacat gtggtcctcc
    20521 ttttccacct tcgtagcgcc ggcgtttgcg gctaccacca ttatgctcgt ggtttttccg
    20581 ctccgggggg tgtaccagat tcgacagaag ttgtcagtgc tcgactacgt accgctctta
    20641 gtcggtggtt actcgctggt cattgccggt gtcatcatcc ttgcattttt ctttcagttc
    20701 agcccatccc gacttatctt tctttatgtc gggatattcg gccttggatt catgtttgcc
    20761 catcgtcttg tggcagcgtt tgtgaggaga tggttcctgc agagaggcat tggcgtggac
    20821 cgtgtcctca tcgtcggtga gggaaaaggc ggccggcggc tcatgcaagc aatgctcgga
    20881 aataggggtc tgggttatcg gcttgtcggt tacgtgggcg acgcctcgcg tggcgatcgt
    20941 ctcaatgtcg ccaccgagcg gggaattctc acgtgtcccc gcctgggagg tctggacgac
    21001 gttggagagt tggtcgtccg cttacacgtg gacgaggtcg tcgtcgttga tgatggcaag
    21061 ggtgatatcg acatcaagcg ggtgctcgac gagtgtcgga acactgacgc ccaattccgg
    21121 attgtgcctg atcttctgca gatctcgctt gatcgtgttg acttctccga aatcggaggc
    21181 gtccccacca tcggtgtgcg ggacgcctcg atccgcggtt ggcatgcagt cattaagcgc
    21241 acggcagaca ttatcgtcgc cttgatggtg tgcgtaattg caatgatccc gatggcgatt
    21301 gtcgcggtgt tgataaaacg cgactcagag ggtccgatct tctatagaca gagtcgcgtt
    21361 gggcaatatg gccagcattt caccatgatc aagttcaggt gtatggtcaa taacgcggat
    21421 cagcaatggg ctgagatggt gaaatcaatg cagggagggg atggccggct cttcaaggat
    21481 ccgaacgacc cccggatgac caaagtcgga aagacgctcc ggcgctacag cattgacgag
    21541 ctcccgcaaa tcttcaatgt attgcgaggg gagatgagtg ttgtggggcc acgcccgcca
    21601 ctccagaggg aggtcgatca atatgacgaa tggcatatgc agaggctgct cgtgcgcccg
    21661 gggcttaccg gactgtggca ggtcaacgga cgaagcaagc tcgccttcga cgagatggtc
    21721 cggctggacc tctactatgc agagaactgg accccgtggc tggacgtaaa gatcgtactc
    21781 cggaccattc ctgcggtcct cttggggcgg ggggcgtact agtctgatgg atctctggtc
    21841 gtatctgagg gcgctgctcc gctggtggtg gtggctggtg ctggttcccc tgatcgcctt
    21901 cgttctggcc tttttcgttc ttttccccga agctccctgg cagacaacgt ggaaaacggt
    21961 tatcaccttc gaaggcaacc cggcgaaggc gagcagcttt gagtacgttg acttcatcgt
    22021 ccttgacgat atggagcacc tgctgcagtc cgatgttatc ggcgatcaag tatatctgca
    22081 tctaccagat gagattacct cccggtattc ccgcgaagat atcggacaga tgtactcgtc
    22141 ctatcgccac gcgcgttttg tacaaatctg ggttaccggt gacgaacccg aggtagtcga
    22201 ggcagtggct caagccaccg aggcggtgtt gcctgaagct gttaacgaat acctgattcc
    22261 tcccgataac ccggactttc ccggcatggt agaaaccgtg gaccggctgg gtgagcccgt
    22321 gcaacttgcg atggagcgct ggcagaaggt tgccgcagta accggtgtcg gggtaattac
    22381 ggcactctgc ctcgtcggta ttgtggaatg gttgagcatg agtcatcggc taaagtacgg
    22441 tgagaggtag tcacgggtct ggagatcctc caggtccttg aggatcggtg cgaggggatc
    22501 gacagcgagc gtgagacacg tcgtggcgat cagcgcaaag agctcgtgtg cggtcgaagg
    22561 ggtgaatgcg tctgaccgca aaccttcggc ggtgacgcga agtaactttg cgacagtgct
    22621 ccgggcgggc aacgattcag agttcgatct gtcgaacgcg cggtccggcc gtacgtcgtt
    22681 ccaggtgaat cccgagcgaa ttgcctcgac cgcggtccag tagctgacct gactggcttc
    22741 caacactatg tcatcgcgtt ggttcgtgtg accgtgggaa ccgtcaatcg tgcccgccag
    22801 ttcccggagt tcgtcctgta tccgctccag tactgaagtt ccagagtttc tcaatagccg
    22861 cgatgtacca gattgtgact cgaggtcctg ctcccggagg aactcataac caccccacca
    22921 acgacgcgtc aagtccgcca caccgttgtg agtgccataa acgtccaggg gatcaaacca
    22981 acgttcccgc actcgctgaa gagagttatc ggcgtccagc cgacggtaat agcaggttgg
    23041 gtagccatca tggcagacgg caccagtttg ctggacctcg atcaagaggc tgttctgttc
    23101 gcagttaacg tagatattct ctacatgctg aacatgccct gaggtttcgc ccttcttcca
    23161 gagcttctcc cgggaccgac tccagaagtg aaccaagccg gtttccctgg tgcgagtcaa
    23221 cgcggtctcg ttcataaagc ccaccatgag tacatggccg gaagtgacat cctcgatgac
    23281 ggctggaatg agtccatcct ctggccactt gatgggcagg tcactgctca cgctgcgtcg
    23341 tccagccgga cggggattcc ccggcctcgg agatagcgct ttacgtcgcc aatgcggagt
    23401 gtgccgaagt ggaagagcga ggccgcaagc acggcgtctg cccggccggg cacgagggcc
    23461 tcatggaaat gctccaggct gccagcgcca cc
//
LOCUS       DASYWM010000260         4284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_349567, whole genome shotgun sequence.
ACCESSION   DASYWM010000260 DASYWM010000000
VERSION     DASYWM010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349567"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VGR29_10630"
     CDS             <1..489
                     /locus_tag="VGR29_10630"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV2074083.1"
                     /translation="TLSVEGRTWINSDGRRNFPSGEIFTGPVEDSVNGHIRFSFPLVT
                     AGRQIEDIRLRFRDGKVVDATAAKNEDYLVQTLDTDEGARYLGEFAFGTNFDIQQFTK
                     NILFDEKIGGTVHMAVGRGYPETGSTNQSAIHWDMICDLRQGGQITVDGELFQKEGRF
                     VV"
     gene            complement(564..881)
                     /locus_tag="VGR29_10635"
     CDS             complement(564..881)
                     /locus_tag="VGR29_10635"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEV2074084.1"
                     /translation="MLFYCAFTWFPGTTRKDVAERVVRQHESGKNKPENIKGWYNLVG
                     GGSGFLVVDYDDPRELTAFLQPYMDVMSFDVRAITENTYEGTVEDMKKFLSGASDNDP
                     IAL"
     gene            1047..2105
                     /locus_tag="VGR29_10640"
     CDS             1047..2105
                     /locus_tag="VGR29_10640"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV2074085.1"
                     /translation="MIDTHDEATLIVTSLLIAEARTVTPVERWLVLGGDGALVAQVAG
                     MGGEVHWRAVDAREVARAPAGVVVTGAIDSETYDSVLLPLPADRNLARRWLLQASDGL
                     EPGGVLILAGANAEGAKSVTADAARVFGAPVAEHYRQKHRIARFRKTEEARDLPAWAS
                     AGGIVPGTWQQFDVDLGEDVVTLETQPGVFAGNRLDAGTRLLLQHLEVEQGARVLDVG
                     CGAGAIGIAASRLGAGHVDLVDANLLAVETSERNLERLGVPGRALASDVFSGVPNARY
                     DLIVSNPPFHRGKQVDYSVANRLIGEAPEHVMRGGRLLVVANAFLAYGKQMERVFRQV
                     ETIAATRQYHILAGTEPR"
     gene            complement(2407..2994)
                     /locus_tag="VGR29_10645"
     CDS             complement(2407..2994)
                     /locus_tag="VGR29_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEV2074086.1"
                     /translation="MKSVAVFCGSSTGTSPEYVDLARALGREIAGRNLTLVFGGGRVG
                     LMGAVADAALKAGGEVIGVIPQALMDKELGHTGISQLRITGSMHERKALMAQEADGFI
                     ALPGGFGTLDEFCEILTWAQLGIHEKPCGLLDTGNGFFRHLRAFFDDAVEAGFVRADH
                     RAMVLEDTDPASLLNRMAAWAPTTTPKWISETPVP"
     gene            complement(3099..3617)
                     /locus_tag="VGR29_10650"
     CDS             complement(3099..3617)
                     /locus_tag="VGR29_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074087.1"
                     /translation="MTESTVESFVLEDDGTFPNNSLPVILYHQAIPLDWHDPARSIEE
                     RFVENGWSGLWRNGIFDFHHYHATAHEVLGCAWGTVQVQLGGPGGRSVSLHAGDVVVL
                     PAGTAHRNLGHSDDYQIVGAYPPGQSPDMCYGNPGERPDAERQIDAVSLPESDPVLGQ
                     HGPLMEQWKTDR"
     gene            complement(3723..>4284)
                     /locus_tag="VGR29_10655"
     CDS             complement(3723..>4284)
                     /locus_tag="VGR29_10655"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEV2074088.1"
                     /translation="SNRIQNGGMGAALLGGGLLLADVLLPIPSSVIMIAHGVAFGIAR
                     GTLLSLTTSVGGAMIGWYIGHRGRGLLGRIVSPAEQENAALLIRRWGMTATVISRFLP
                     VVAETVAVMSGTTDLGWKRVLLAMTIGALPPALIYAIAGTLATDLASGVLVAVSVMFL
                     AAVAWFLGRRLTAPASPAREPTPGQP"
BASE COUNT          803 a         1271 c         1422 g          788 t
ORIGIN      
        1 acgctctcgg tggaggggcg gacatggatc aactccgacg ggcgccggaa cttccccagc
       61 ggggagatct ttaccgggcc ggtcgaggat tccgtcaacg ggcatatccg gttctccttt
      121 ccgctcgtca cggctggacg ccagattgag gacattcgcc tgcgcttcag ggatggcaag
      181 gttgtggatg ccaccgctgc gaagaatgag gactacctgg tccagacact ggatacggac
      241 gagggcgcgc gctacctcgg cgagttcgca ttcggaacaa atttcgatat ccagcagttc
      301 acgaagaaca tcctcttcga tgagaagatc ggtggcacgg tccatatggc ggtcggacgc
      361 ggttatcccg aaaccgggag caccaaccag tccgccatcc attgggacat gatctgcgac
      421 ctgcggcagg gcggacagat caccgtcgat ggggagctct tccagaagga gggcaggttt
      481 gtcgtctagc gcgtctgaag acgaggagtg acaggtacga caagagggca ggggccgcgg
      541 cccctgcccc gccattcaca gggctagagc gcgatcgggt cgttgtccga ggcgccgctc
      601 aggaacttct tcatgtcctc gacggttccc tcgtaggtgt tctcggtgat ggcgcggaca
      661 tcgaagctca tgacatccat gtagggttgc agaaatgccg tcaactcgcg ggggtcgtca
      721 tagtcgacaa cgaggaagcc ggagccacca ccgacgaggt tgtaccaccc cttgatgttt
      781 tccggtttgt tcttgcccga ctcgtgctgt cgcacgacgc gctcggcgac atccttgcga
      841 gtggtgccgg gaaaccaggt gaatgcgcaa tagaagagca ttgttctccc tttctccgca
      901 cagggcgcat cgctgcgtcc tgcacatttt cctgacaaca gtccggaggt cagcgacgat
      961 ctccattgca taatggccag cacaacgcat gccaacttca cgctgacggc ttagcggcag
     1021 tcggaacggg tggacccgga gaactggtga tcgacactca cgacgaagcg accttaatcg
     1081 tcacatccct gctcatcgcc gaggcacgaa ccgtcacgcc ggtggagcga tggctcgtgc
     1141 ttggtgggga cggcgccctt gtggcccagg tggccggtat gggaggggag gtgcattggc
     1201 gagcggtgga tgcccgggag gtggcccgtg cgccagcagg tgtggtggtg accggtgcta
     1261 tcgactccga gacgtacgat tctgtattgc ttcctctccc ggccgatcgt aaccttgcgc
     1321 ggcggtggct gctgcaggcg agcgatgggc tggagccggg tggggtgctg atccttgccg
     1381 gggcgaatgc ggaaggcgcg aaatcggtca ccgcagacgc cgcgcgggtc ttcggtgcgc
     1441 ccgttgccga gcattatcgc cagaagcacc gcatcgcccg cttcaggaag acggaagagg
     1501 cccgtgatct gccagcgtgg gcgagtgcgg gcggtatcgt gcccggaacc tggcaacagt
     1561 tcgatgtcga tttgggcgag gatgtggtga cgctggagac gcaaccgggt gtctttgccg
     1621 ggaatcggct ggatgctgga acccggctgc tgctccagca tctggaggtc gagcagggtg
     1681 cgagggttct cgatgtcggt tgtggcgctg gtgccatcgg gattgcggcc agccggctgg
     1741 gggcggggca cgtcgacctc gtcgatgcga acctcctcgc ggtcgagacc tcggaacgga
     1801 accttgagcg gttgggggtg ccgggccgcg cactcgcaag cgatgtcttc tctggcgtcc
     1861 ccaacgcgcg atatgacctg atcgtgagca acccgccatt tcaccggggc aagcaggtgg
     1921 actactcggt cgccaaccgg ctgatcggcg aagctccaga gcatgtgatg cggggaggtc
     1981 gcctgctcgt cgttgcgaat gcgttcctgg catacgggaa gcagatggag cgggtctttc
     2041 gacaggtgga gacgatcgcc gcaacacggc agtaccacat cctggcgggg acagagccaa
     2101 ggtaacagtg gctcccccac gtgcggttaa ggggcggaca ggtgaggttc cagatccagc
     2161 ccacgactcc tccaggacag acggtatcta aaggagtcac gtatgccggg ttggagcgag
     2221 cacgttggcg ggggacttgc ggccagcgga gatgcgtcga cagccacacc aatgaggcgg
     2281 cggcgcatgc gcttcggtcg gggattcctc gctctgcccg gaatgacgga aagcgcaaga
     2341 ccgacgcgga ttgtgcggaa gagaagacat ccaggcgggg acagagccga ggtaatgcgg
     2401 gagcacctat gggaccgggg tttcgcttat ccacttcggg gtagttgtcg gcgcccaggc
     2461 ggccatgcgg ttgagcaggg aagccgggtc tgtgtcctcc agcaccatcg cgcggtgatc
     2521 tgcccggacg aatccggcct cgaccgcatc atcgaagaag gcgcgcaggt gccggaagaa
     2581 cccgttgccg gtatcgagga ggccgcacgg cttttcgtgg atgccgagct gggcccatgt
     2641 caggatctcg cagaactcgt caagcgtgcc gaagcctccc gggagcgcaa tgaagccgtc
     2701 cgcttcctgc gccatcaatg cctttcgctc gtgcattgat ccggtgatgc ggagttgcga
     2761 gatgccggtg tgcccgagct ccttgtccat caacgcttgg ggaatcaccc cgatcacttc
     2821 tccaccagcc ttcagggccg catccgcaac ggctcccatg agtccgaccc ggcctccacc
     2881 aaagacgagg gtgagatttc ggcccgcgat ctcgcggccc agcgcccggg cgagatcgac
     2941 atactcagga ctggtgccgg tgctggagcc acagaagacc gctactgatt tcatgaacca
     3001 actccatctg aaatccgcgt gataaggctt caccgacgat aaatcccatc ggtttcggcc
     3061 atcgcgacag gaatgctttt cttcgaatcc ggctgcggtc accgatcggt cttccactgt
     3121 tccatgaggg gcccgtgctg gccgaggacc gggtcgctct ctggcaggga gacggcgtcg
     3181 atctgcctct cggcatcggg acgctcccct ggattgccat agcacatgtc aggcgactgg
     3241 ccaggcggat acgcaccgac gatctgatag tcatcactgt gcccgaggtt ccggtgtgcc
     3301 gttccggccg ggagcacgac gacatctccg gcgtggaggc tgacacttcg gccgccgggt
     3361 ccgccaagct gaacctggac cgtcccccag gcgcacccga gcacctcgtg ggcggtggcg
     3421 tgatagtggt ggaagtcgaa gatgccattt cgccagagac cactccagcc gttctcgaca
     3481 aagcgttcct caatggaccg ggccgggtcg tgccagtcaa gagggattgc ctggtggtag
     3541 aggatcacgg gcagagagtt attcggaaag gtgccgtcat cttcaaggac gaaggattcg
     3601 acggttgatt ctgtcattgc agtgcctccg tttcccagga aaggcttgtt tgcatcgcta
     3661 tcccgattgc acttcattgc agggattgcc cttgggcatg cgaagcagtt tgcgatccat
     3721 gattatggtt ggccgggagt tggttcccgt gccggagacg ccggtgctgt cagtctcctc
     3781 ccaaggaacc aggcgaccgc cgccaggaac atgactgaca cggcgacgag tactccactc
     3841 gcgagatcgg tggccagggt gccggcgatc gcgtagatga gtgccggggg gagggccccg
     3901 atcgtcatcg caaggagcac cctcttccat ccgaggtccg ttgtgccgga catcaccgca
     3961 accgtctccg cgacgacggg tagaaagcgg gaaataacag tcgctgtcat tccccagcgg
     4021 cggatcaaca gggcggcgtt ctcctgctcg gccggcgaga cgatgcgccc caacaatccc
     4081 ctgccgcgat gcccgatgta ccagccgatc atggcgccac cgacgctggt ggtcagcgag
     4141 agcagggtcc ccctcgcgat cccgaaagcc actccgtggg caatcatgat tacgctcgag
     4201 gggatgggca ggaggacatc agccagcagg agaccgccgc cgagcagcgc ggcgcccatg
     4261 ccgccattct ggatcctgtt ggac
//
LOCUS       DASYWM010000261         6714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_143030, whole genome shotgun sequence.
ACCESSION   DASYWM010000261 DASYWM010000000
VERSION     DASYWM010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143030"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1693)
                     /locus_tag="VGR29_10660"
     CDS             complement(35..1693)
                     /locus_tag="VGR29_10660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV2074089.1"
                     /translation="MNESVRVIFLGGIAEVGKNLLVLETDRDIIVVDCGLGFPEEDQP
                     GVDLVLPDIGYLQGKRDKIRGFFITHGHEDHIGALPWLWEDIPAPIHATRLTAGLISV
                     KMREKRLMDRVQLEVFDPDTRPSISAGEFVVEPFRVTHSIPDAVGFGITTPAGLIVIT
                     GDFKFDPTPIDNRPTDVELLAEFGDRDVRLLISDCVNVERDGVTPSERVVGETYQDVF
                     SQAPGRIIIATFASLIARIQQVIDTASRHGRKVAALGRSLENNIRVAVELGYLHDPEN
                     VMVSSKTAEKLPDDEIVYIVTGSQGEPMAVLSRIANRDHPKITVGEGDTVVVSAAPIP
                     GNETSVFRIIDQLFRSGAEVVYTARARVHVSGHAGREELRRMIELTRPEFVIPTHGEH
                     RHLALYADLAVESGIARKNITMVDRGDVVELDEQGIGISEQLDVSSVLVQGSSFGHLD
                     DLVLQDRQAMSQDGLVMVAVGIDAGNGVVVSGPKVATRGFTVVQSRDDLTQGVADAVR
                     SALNARPFGSNSQATNRVIREAASRYVYTQTKRRPMILPLILDI"
     gene            complement(1861..2460)
                     /locus_tag="VGR29_10665"
     CDS             complement(1861..2460)
                     /locus_tag="VGR29_10665"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV2074090.1"
                     /translation="MADVREEVVGCTRCELHRSANNGVPGEGSPDARIVIIGEAPGWH
                     EDKQGKPFVGNSGKFLTELLEKAGLKRDEVFITNVVKHRPPGNRDPLPDEIMACSVYL
                     ERQIAAIDPDVIVTLGRFSMAKYFPGEKISKIHGEAKDVGGRFVVPMYHPAAALHNGA
                     LRSTIEEDFQKLPRLLAERDKQRSVDREKSAQASQGTLF"
     gene            complement(2707..5613)
                     /locus_tag="VGR29_10670"
     CDS             complement(2707..5613)
                     /locus_tag="VGR29_10670"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEV2074091.1"
                     /translation="MRSRSSTRLSISLILAMLIGLSTLVTPVAAQVATPGATPPDIVS
                     VEATPDLPETVEEGTTPEVPAEVEIASPETVLIQPDGATPDPALVASPMASPIASPVT
                     DIGGDVDLDVLFIGAHPDDEAFALSTYGEWNEFNDVEVGVVTITRGEGGGNAVGTEEG
                     PALGLLREDEERRAVGVAGIEHIFNLDKVDFYYTVSAELTAETWDYEDTLERVVRVVR
                     ETQPEVIITMNPAPTPGQHGHHQVAGRLAIDAFYAAADPGVFPDQIDNEDLDAWRASS
                     IFRTRASGEGTPGPECAVNYQATEPTDVVYGVWAGTESEANGKTWAQLEREAQRIYAS
                     QGWADFDDAPIDPAEIECDYFTLIDSRAPFDPANTSSTAILEGAVLPADNGLPLGSEF
                     YLTTDIFNVAPGEPFDVTAHLRAEGVDASTEVAISAPEGWTVGEVGKPVEANGELQWT
                     TQVTPAEDAAIDERVRLEATVTTDDGTAMTAEVVAVTADVTGQLEPLPEVAYFRQWVE
                     EVAVPALDSLIFPVFSIGVGETRDISVDVTNTSEEPASGTVTLELPEGFEAEPVAQEF
                     TDLAPGATEAVSFTVTNTDESLLTSNEGGDEGTYAFTITTSTDAGDSVQNAGINLVPS
                     TTVPKAAATPVIDGAVDTDVYTGESLDLSRVWEGDPVDSPEDGTGTAHVVYTEEGIYV
                     AVEVTDDELGTVLPVADAKRHWRTDSVEIAIDPLGTAGNTSTTFKVGVFPTTEEGEPA
                     AYRDADAYQGPVSQTAPGFELASTVSEPYTGYVVETFIPFEALPADIDPDSATMNIFI
                     YDSDTQDLNGQTRLGWSTWQGVQGDPYRWGKIDFEGFTAPADARTTPDEPIMPLEAAQ
                     SADSPMSVLQSAQDGVPLAGKTPVPEGEGLIVEEVTTEEGEHEITFTAQSGGNVHVFL
                     MVDGEVTGEYRDSVSEGSTRSYAFPEVEGADDAQFVVSFVDEEERVQAIAEPLP"
     gene            complement(5700..6515)
                     /locus_tag="VGR29_10675"
     CDS             complement(5700..6515)
                     /locus_tag="VGR29_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2074092.1"
                     /translation="MIRRWLTYFALLLGCVIAIAPFVVTILASVKTTGELVQGIFSLP
                     EEAQWKNYVEAWNQGRFGRFFLNSIIVVVAVVIPSVVFSAMTGFAFARFRFRGSGFIF
                     AYLLLGLVIPLQALVVPLYYVLRELGLLDSLWALILPQIAMSMSFGTLLMRQAFLSIP
                     KEIMEAAIVDGASSWTTLWQIMYPLARPMIGTVALMFFIWTWNEFLLPLVVNIDPRYH
                     TLPVGLLYFQERWTSNIPIVAAGATIIFLPLVAMFLLFQRQLIAGITQGAVKG"
     gene            complement(6508..>6714)
                     /locus_tag="VGR29_10680"
     CDS             complement(6508..>6714)
                     /locus_tag="VGR29_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074093.1"
                     /translation="NALRAFDIIKATTDGGPGDETQVMALYMVNTAFGANQAGYGTAI
                     AVLLAILTLVGSLLVMRAFGEDDD"
BASE COUNT         1384 a         2122 c         1864 g         1344 t
ORIGIN      
        1 gcaaatcgct gtaggggcag tggaggtcga ggacttagat atcgagaatc aacggcaaga
       61 tcatgggccg acgctttgtc tgagtgtaga cgtatctcga tgccgcctca cggatcaccc
      121 tgtttgttgc ttgtgaatta ctgccgaatg gccgcgcatt gagtgcggac cgtaccgcat
      181 ccgctacacc ttgcgtgaga tcgtccctgg actggaccac ggtaaagccg cgagtagcga
      241 ctttggggcc tgacactacc acgccatttc ccgcatcgat acctaccgcc accatgacga
      301 gcccgtcctg cgacattgcc tgccggtcct gcagcaccag atcatcaaga tgcccgaaag
      361 aggagccttg caccagcacg gagcttacgt cgagttgctc agagatgccg ataccctgct
      421 cgtccagttc cacgacatcg cctcgatcga ccatcgtaat gttcttccgc gcaatcccgc
      481 tctccacagc aagatcggca tacagcgcca ggtggcggtg ctcaccatgt gtcggaatca
      541 caaactccgg ccgtgtcaat tcgatcatcc ggcgcagctc ctcgcgtccg gcatgaccag
      601 aaacatgcac ccgcgcccgt gcggtgtaga caacctcggc accggagcga aagagttggt
      661 cgatgatccg gaaaaccgat gtctcgtttc ccgggattgg ggctgcggag acgacaactg
      721 tgtccccctc ccctaccgtt atcttgggat gatcgcgatt ggcgatcctg ctgagcacag
      781 ccatcggctc accctggctc cccgtcacga tgtaaacgat ctcgtcgtct ggtagcttct
      841 ccgcggtctt ggacgaaacc atgacgttct ccgggtcatg gagatacccg agctccacag
      901 cgactcgaat gttattctcc aggcttcgac cgagagccgc gacctttcga ccgtgccgtg
      961 atgcggtatc gatcacttgc tggatgcgcg cgatgagcga ggcgaatgtg gcgatgataa
     1021 tgcggccggg ggcttgggaa aatacgtcct gataggtctc gcctacaaca cgctccgagg
     1081 gagtcacacc gtcccgctcc acgttcacac agtcggagat cagcagccgg acgtcccggt
     1141 caccgaactc ggcgagaagc tctacatccg tcggccggtt atcgatcggt gttggatcga
     1201 acttgaagtc accggtgatg acgatgagtc ctgcgggcgt cgtgatcccg aaaccaaccg
     1261 catccggaat actgtgtgtg acccggaagg gctcgaccac aaactccccg gccgaaatgg
     1321 agggccgggt gtcaggatcg aagacttcca gctgtacccg gtccatgagt cgcttttcac
     1381 gcatcttgac cgagatgagc cccgccgtga gccgggtcgc atggatagga gcaggaatgt
     1441 cctcccagag ccagggaagc gcgccgatat ggtcctcatg tccgtgagtg atgaagaagc
     1501 cgcggatctt gtccctctta ccctgaaggt atccgatatc tggcagcacc agatccacac
     1561 cgggctgatc ctcctccgga aacccgagac cacagtcgac aacgatgatg tcgcgatcgg
     1621 tctcgagaac cagaagattc ttgccgactt cagcgatacc gccgagaaag attacgcgta
     1681 ccgattcatt cacgcttcag gctatccttg atccccggta acagggcgca tcgtcgtaca
     1741 tctccagggt tcctgttcga cggtcacgct tcgatgaaga agactcccaa acgggcgcgc
     1801 tcttcctgcc gtcagcacca ctacgggtca ccggaagcgt cgggcaacat cagcaccgcc
     1861 ttagaacagc gtcccctggc tcgcctgcgc acttttctcg cggtcgacgc tgcgctgctt
     1921 gtcacgctcc gcaagcagcc tcggcaattt ctggaagtct tcctcgattg tgcttcgcag
     1981 tgcgccattg tggagtgccg cggccggatg atacatcggc accacgaagc gccccccgac
     2041 gtccttggcc tcaccgtgga tcttgctgat cttttcgccc gggaagtatt tcgccatgga
     2101 aaaccgtcca agagtcacga tcacgtcggg atcaatggcc gcgatctggc gctccaagta
     2161 taccgagcag gccatgatct catccggaag agggtcgcga tttcccggcg gacggtgttt
     2221 caccacgttc gtaatgaaca cttcgtcgcg cttcagcccc gccttctcaa ggagctcggt
     2281 caggaacttg cccgagttcc ccacgaacgg tttaccctgc ttatcctcgt gccagcccgg
     2341 cgcctccccg ataatgacga ttcgcgcatc cggactgcct tcgccgggaa cgccgttgtt
     2401 cgcggagcga tgcagctcgc accgggtgca gcccactacc tcttcccgaa catccgccaa
     2461 cacccgttcc gccgaacctt cagacattgt cgtacctcca ttaatctccg aacgtgagta
     2521 tagatcgagt atcgcgagca tggacacggc cggcgcggca ctgctccccg acatgaattg
     2581 caagagagca cctgggccaa aggcgggatc gcctcctttc acacactctg ccgataccac
     2641 gcaaagagag ggcccccgga tattccgggg gccctctcgc ttgctaccat gcacatcgag
     2701 ataagactag ggcagcggtt ctgctatcgc ctgcacccgt tcctcttcat cgacgaacga
     2761 cacgacgaac tgtgcgtcat cagcgccctc tacctccggg aacgcgtagc tgcgggtcga
     2821 gccctcgctg acactatccc ggtactcgcc agtgacctcg ccgtccacca tgaggaagac
     2881 atgaacgttt ccacctgact gggcagtaaa ggtgatctca tgttcgccct cctccgtggt
     2941 cacctcctcg acaatgagtc cttctccttc cgggacagga gtcttgccgg cgagcgggac
     3001 tccgtcttgg gcggactgca ggacagacat cggggagtcg gcactttgcg ccgcttctag
     3061 cggcatgatc ggctcgtcgg gagtcgtgcg ggcatctgcc ggtgctgtga atccctcgaa
     3121 gtcgatcttg ccccagcggt acgggtcgcc ttggacgccc tgccaggttg accaccccag
     3181 acgcgtctgc ccgttaaggt cttgcgtgtc ggaatcgtag atgaagatgt tcatcgtcgc
     3241 gctgtcgggg tcgatgtccg ccggcagagc ctcaaacgga atgaaagtct ctacgacata
     3301 accggtgtac ggttcgctta ccgtgcttgc gagttcgaac ccgggtgcgg tctggctcac
     3361 cggaccttgg tacgcatcgg catcacggta ggccgcaggc tctccctctt ccgtcgtggg
     3421 gaagacaccc accttgaaag tcgtggatgt attgcctgcc gtgccaagcg ggtcgatcgc
     3481 aatctcgacg gaatctgtgc gccagtgccg cttggcgtcg gcgactggca ggactgtgcc
     3541 cagctcgtca tccgtcacct caacagcgac atagatgcct tcttcggtgt agacgacatg
     3601 tgccgtccca gtgccgtcct ctggcgaatc gaccgggtca ccttcccaga cgcggctgag
     3661 gtcgagcgat tccccggtat agacgtcagt atcgacagct ccatcgatga cgggtgtcgc
     3721 ggcagccttc ggcaccgtcg tgctcgggac cagattgatg ccggcattct gcaccgagtc
     3781 gcccgcatcg gtggaagttg tgatcgtgaa ggcgtaggta ccttcgtcgc caccttcgtt
     3841 cgaagtcagc agcgattcat cggtgttggt gaccgtaaag gatactgcct ccgtcgcgcc
     3901 gggcgcgaga tcggtaaatt cctgtgcaac cggctctgcc tcgaagccct cgggcaactc
     3961 caacgttacc gttccggatg cgggttcctc cgatgtattt gtgacatcga ccgagatatc
     4021 ccgggtctcg cccacgccaa tggaaaagac aggaaagatg aggctgtcaa gcgccggcac
     4081 ggcgacctct tctacccatt gacggaagta cgcgacttct ggcaacggct caagctgacc
     4141 agtgacatcg gccgtaactg caactacctc cgcggtcatc gccgtgccgt cgtcggtcgt
     4201 gaccgtagct tccagtcgga cacgctcgtc gattgcagca tcctccgcag gcgttacctg
     4261 cgttgtccac tgcagctcac cattcgcctc aaccggcttg cctacctcgc cgacggtcca
     4321 gccttccggt gcggaaatgg ccacctcggt cgaggcatca actccttcag cccgcaggtg
     4381 cgccgtaacg tcgaatggtt cgcccggcgc gacattgaag atgtcagttg tcaggtagaa
     4441 ctcggagcca agcggcagac cgttatccgc cggaaggact gctccctcca ggatcgccgt
     4501 ggacgacgta ttcgcaggat cgaatggtgc cctgctatcg atcagcgtga agtagtcgca
     4561 ttcgatctcg gcgggatcga taggggcatc atcgaagtct gcccaccctt gagacgcata
     4621 gattcgctgg gcctcgcgtt cgagctgcgc ccacgttttg ccattcgcct ctgactctgt
     4681 accagcccaa acgccataga cgacgtcggt cggctcagtc gcctgatagt ttacggcaca
     4741 ctctggccct ggagtgccct caccggaggc acgagtcctg aagatgctgg aggcccgcca
     4801 cgcatccaga tcctcattgt cgatctggtc agggaatacc cccgggtcag cggcagcgta
     4861 gaatgcatcg attgccaggc gacccgccac ctggtggtgg ccatgctggc cgggggtcgg
     4921 cgccggattc atcgtgatga tgacctccgg ctgcgtctct ctcacaacac ggacaacccg
     4981 ctccagcgtg tcctcatagt cccacgtttc ggcggtaagc tctgcgctca ccgtgtagta
     5041 gaagtcaact ttgtcgagat tgaagatgtg ctcgattccc gccacgccaa cggcgcgacg
     5101 ctcctcgtcc tcacgcagga gcccgagcgc aggcccttcc tcggtgccga ccgcgttgcc
     5161 accgccttca ccccgcgtga tggtgaccac accgacctcg acatcgttga actcgttcca
     5221 ttcgccatac gtgctcagcg cgaacgcctc atcatccggg tgagcaccga tgaaaagcac
     5281 atcgagatcg acatcgccac cgatatctgt caccggtgaa gcaatgggcg atgccattgg
     5341 cgaagccacg agggcaggat ccggagtggc tccatccggc tggatcagca cagtctctgg
     5401 agaggcaatc tccacttccg ccggcacctc cggtgttgtg ccctcttcca ccgtttcagg
     5461 taggtcgggc gttgcctcca cagaaacaat gtccggtggt gtggcgcccg gcgttgccac
     5521 ttgtgcagcg accggagtta cgagggtact cagaccgatt agcatcgcaa ggattagcga
     5581 tatgcttaaa cgggtactgg atcgactccg catgctcaac ctccttaaac tcgtaccgtt
     5641 atgtcggggt caccatacga ccatagaacc tccgatctga caagccttgc caaaagagat
     5701 caacccttga ccgccccctg ggtaataccc gcgataagct gcctctggaa gagcaggaac
     5761 atcgctacga gtggaaggaa aatgatcgtc gcgcccgcgg cgacgatggg gatattggat
     5821 gtccagcgct cctggaaata gagcaaaccc acgggcaagg tgtggtacct cggatcaata
     5881 ttgaccacta acggcagcag gaattcgttc caggtccaga tgaagaacat cagcgccacg
     5941 gtaccgatca tcgggcgcgc gagcgggtac atgatctgcc aaagcgtggt ccacgacgat
     6001 gcaccgtcca cgatcgcagc ttccatgatt tctttaggga tcgagaggaa cgcctgcctc
     6061 atcaatagtg tgccgaacga catgctcatc gcgatctgcg gcaagatcaa cgcccacagg
     6121 ctgtcaagca accccaattc ccggagcacg taatagagcg gcactaccag tgcctgcaat
     6181 ggaatcacga gaccaaggag caaatacgcg aagatgaatc ccgagccccg gaaacgaaat
     6241 cgggcgaaag caaatcccgt catcgcggag aaaacgacgg acggaatcac aaccgcaacc
     6301 accacaataa tgctgttcag gaagaatcgg ccgaagcgtc cctgattcca ggcctccaca
     6361 tagttcttcc actgagcttc ttcgggaagg gagaatatcc cctgcaccaa ctcgcccgtc
     6421 gtctttactg aagccaggat cgtgacgacg aacggagcaa tcgcgatcac acatccgagt
     6481 aggagcgcga aataggtcaa ccaccgtcta atcatcgtct tcaccaaatg cacgcatgac
     6541 cagtagagag cctacaaggg tcagaatcgc cagcaggaca gcgatcgctg ttccgtaacc
     6601 cgcctggttc gcgccgaagg cggtgttgac catatagaga gccatcacct gcgtttcatc
     6661 gcccggtcca ccatccgttg tggccttgat gatgtcgaac gcccgtaacg cgtt
//
LOCUS       DASYWM010000262         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_199890, whole genome shotgun sequence.
ACCESSION   DASYWM010000262 DASYWM010000000
VERSION     DASYWM010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199890"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1580)
                     /locus_tag="VGR29_10685"
     CDS             complement(<1..1580)
                     /locus_tag="VGR29_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV2074094.1"
                     /translation="MIDTQTKPVTIEEIAGRLIPGAPQISPDGRHVAFTVATSGRKEK
                     RHNRAIWVSIDGQPARRFTGGSGNNQSPRWSPDSSQILFHAQREEDDHHRLYLIPVHG
                     GEAQQLGDLKGSMSAPRWSPDGAEVSVLLSDTESEDEKKRKEEKDDAVVFEDQDKVDR
                     LWVVEVASGKAKCLTHGKRHVRDYAWSPDGEHLVLVTSDSPTANEMFRSSSLRLIPSA
                     GGTSRHLADFRNLPSDPVIRTVDGDQVVAFVGNDHREDPSYAVWTVPLNGGAKRKLIE
                     NDRAATEFLIADPASPDGLILGQADGAQVRQYRLSIRSGELTPIPMQGLGDRGSIQSA
                     PSVALAAGEMAVVWTAIDVPEEVYRVSSEGRAARLTSFGEALQGRLSGGELVTWESDD
                     GVDIEGVLVYPRGYEEGTRYPLLVQVHGGPAWHWQDRINMSWHDWAQMMAAKGFAVLL
                     PNPRGSIGYGSPFEQLLQDDVGGGESQDLVSGALVMVERGIADRDRLAIGGWSWGGYL
                     TAWTITQTDIFRAAVMGAG"
     gene            complement(1627..>3639)
                     /locus_tag="VGR29_10690"
     CDS             complement(1627..>3639)
                     /locus_tag="VGR29_10690"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV2074095.1"
                     /translation="EFRLAVEEAVRPFRNDPDVAAVYTWGSTSSDTLNTTFISADRTQ
                     SLAILVMAHTATPGASSLEDIRSRLASDTLEIQIGGWPATAEAFLGLAGSDLARAEII
                     SLPITLILLLVIFGSVIAAGLPIILALLSMIATLAALALLSRVMLVNIFSINAVTMLG
                     LAVGIDYALIMVSRFREETTSTAISESIPRIVDTAGRAVLIAGSTVAIGLTGLLIFGV
                     PAAISTGLAGAFVVLTCVALALTALPASFVLWGHRISRRNINFARYPRFLTLVPNQVQ
                     ALRKRYPFPVVIVCGLLLMSLALPLRQLVVSSPTMTILPRTSEPRIVYDTIAESFPNA
                     TLSPISIIVEPQSGTMMSAPNLAAVRTLTTNLGDTPGVSSAESVWEFIPDSISVETFT
                     TSLLIEPEMVRAAAPLLTSDAALINLTPDPGLDAAQRRDLVEDLRAAMPSLTESSLTV
                     RIGGDAGLDLDLMNHVKQRAPLVVGFVIGLTWLALLVQFRSLFLPLKAILLNLISLSA
                     SFGALVWIFQEGHLSNVLAFEPLGYTVVLVPILMFCFLFGLSMDFEVLMLSRIREAWE
                     ETGDNSRAIDLGLHRSAGIVTSSALVMLVVFSAFGTSQLQLVKSLGVGLGLAVFIDAT
                     IIRMLLLPATMQLMGRWNWWLPFASERKSGHSERQPLVHEHRASSS"
BASE COUNT          747 a         1055 c         1163 g          674 t
ORIGIN      
        1 cccgcgccca tgaccgcagc acggaaaatg tcggtctggg tgatcgtcca cgcggtgaga
       61 tagcctcccc aggaccaccc gccgatggcg agccggtcgc ggtcggcgat gcctcgctcg
      121 accatcacca aggcaccgct gaccagatcc tgcgactccc cgccacctac gtcatcctgc
      181 aggagctgtt cgaacggtga tccgtagccg atcgacccgc gcgggttcgg gagtagcacc
      241 gcgaatccct tcgccgccat catctgtgcc cagtcgtgcc agctcatgtt gatccggtct
      301 tgccagtgcc aagccggccc tccatgaacc tggactagca agggatagcg tgttccttcc
      361 tcgtagccgc gtgggtaaac aagcactccc tcgatgtcca cgccgtcatc gctctcccaa
      421 gtcaccagct cgccgcctga cagtcgtcct tgcaacgcct cgccaaagga tgtcaggcgg
      481 gcagccctgc cttcgctgga gactcgatag acctcctccg gtacatcgat tgccgtccag
      541 acgactgcca tttcgccagc tgccaaggcg accgacgggg cgctctggat actgccgcga
      601 tcacccaagc cctgcatcgg gatcggcgtc agctctccgg aacgtatcga gaggcgatac
      661 tggcgcacct gcgcaccgtc agcctgaccc aagatcagcc cgtcgggaga agctgggtca
      721 gcgatgagga actcagttgc cgcgcggtcg ttctcgatca gtttccgctt tgccccacca
      781 ttaagcggaa ctgtccagac ggcgtaggag ggatcctccc ggtggtcgtt tccaacaaag
      841 gcgacaacct ggtctccgtc gacggtgcgg atgacggggt cgctggggag attgcgaaaa
      901 tcggcaaggt gacgtgatgt gccgccggcg gatggaatga ggcgaagcga agatgaccgg
      961 aacatctcat tcgcggttgg ggagtcgctg gtgaccagga caagatgctc accatctggt
     1021 gaccatgcgt agtcccgcac gtggcgcttc ccgtgcgtca ggcatttcgc cttgccgctt
     1081 gccacctcaa cgacccagag ccggtcaacc ttatcctggt cctcaaagac gactgcatcg
     1141 tccttctctt ccttgcgttt cttctcgtcc tcgctttcgg tatcggagag gaggaccgag
     1201 acctccgctc catctggcga ccaccggggt gcgctcatgg agcccttgag atcgccgagc
     1261 tgttgcgctt cgccgccatg aaccgggatc aggtagagac ggtgatggtc gtcctcttcc
     1321 cgctgcgcat ggaagaggat ctgcgagctg tcgggagacc agcgcgggct ctggttgttc
     1381 cccgagccac cggtaaaacg gcgcgcgggt tgcccgtcta tggagaccca gatcgcgcga
     1441 ttatggcgct tctccttgcg gccactagtg gccacagtaa aggcaacgtg tcgaccatcg
     1501 ggcgagatct gcggtgcgcc ggggatgagt cggccagcga tctcctcgat cgtgactggt
     1561 ttggtctggg tatcgatcat ctgttgaatt ctccgatact gggtcttcta actcttgcga
     1621 ttgcaatcag gatgagctgg cgcgatgttc gtggacaagg ggttggcgtt ccgagtggcc
     1681 gctcttccgt tcactggcga atggaagcca ccagttccag cgtcccatca gctgcatggt
     1741 agccgggagc agtagcatcc gtatgatcgt cgcatcgatg aagaccgcaa ggccaagacc
     1801 cacgccgagg gatttgacaa gttgaagttg gctggtgccg aatgcggaaa agacgaccag
     1861 cataacgaga gcagaggagg tgacaatgcc cgctgagcgg tgcaacccca gatcgatggc
     1921 tcggctgttg tctccggtct cctcccaggc ttcccggatg cgtgagagca taaggacctc
     1981 gaaatccatg ctgagaccga agaggaagca gaacatcagg atggggacca acacaaccgt
     2041 gtagccgaga ggctcgaacg cgagcacgtt cgagaggtga ccctcctgga agatccagac
     2101 aagcgcaccg aacgatgccg acagcgagat gaggttgagg aggatcgcct tcagggggag
     2161 aaagagtgag cggaattgta ccaagagcgc cagccaggtg agcccgatga cgaacccaac
     2221 gacgagcggt gcccgctgct tcacatgatt cataagatcg aggtcgagcc cggcatcgcc
     2281 cccgatgcgg actgtgaggc tgctctcagt aagcgacggc attgccgcgc ggagatcctc
     2341 cactagatcg cggcgttgcg ccgcatccag cccggggtct ggcgtcaggt tgatcagggc
     2401 ggcatcgctc gtgagaagag gagcggcggc ccgcaccatc tccggctcaa tcaggagact
     2461 cgtcgtgaag gtctcaaccg aaatactatc cgggataaat tcccagacac tctccgccga
     2521 ggagacacct ggcgtgtcac caagattcgt tgtgagcgta cggactgccg caaggttggg
     2581 tgcgctcatc atcgtgccgg attgcggttc gacgatgatc gagatcggcg agagtgtcgc
     2641 gttcggaaat gactccgcga tggtgtcata aacgatccgt ggctcactgg ttcgcgggag
     2701 gatggtcatc gtgggcgatg agacgaccag ttggcgtagt ggcagggcga gtgacatgag
     2761 caggagcccg cacacaataa cgacggggaa tgggtagcgc ttccggagtg cctggacttg
     2821 attgggaacc aacgtcagga atcgtgggta tcgagcgaag ttaatgtttc gccggctaat
     2881 gcggtgtccc cacaggacga aggatgccgg cagtgcggtc agggcaagcg cgacgcaggt
     2941 gagtactaca aaggctcctg ccaagccggt tgaaatggcg gctggcacac cgaagatgag
     3001 gagtcccgtc aggccgatcg cgacggtgga gccagcgatg agaaccgcgc ggccagcagt
     3061 atcgacaatc ctcgggattg attcactgat tgctgttgat gtggtttcct cccggaagcg
     3121 gctgaccatg atcaatgcat agtcgatgcc gacagcgagg ccaagcatcg tgacggcgtt
     3181 gatcgagaag atattgacca gcatgacccg ggagagcagt gccagggcgg cgagagtggc
     3241 gatcatggaa agcagggcca ggatgatcgg caaaccggcc gcgattacgc ttccaaagat
     3301 tacgagcagc agaatcaggg tgattggaag ggagatgatt tcggcgcggg cgagatcaga
     3361 gccggcaaga ccaagaaacg cctctgcggt ggcaggccat ccgccgattt ggatctccag
     3421 cgtgtcgctg gcgaggcggg agcggatatc ctccaggctg ctggctcccg gcgtcgccgt
     3481 gtgcgccatg acgaggatag ccaggctctg tgtgcggtcc gccgagatga aggtggtatt
     3541 cagggtgtcg cttgaagtcg agccccaggt gtagacggcg gcaacatccg ggtcgttccg
     3601 aaaggggcgt accgcctctt cgaccgcaag tcggaactc
//
LOCUS       DASYWM010000263        10534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_462923, whole genome shotgun sequence.
ACCESSION   DASYWM010000263 DASYWM010000000
VERSION     DASYWM010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462923"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1787)
                     /locus_tag="VGR29_10695"
     CDS             complement(279..1787)
                     /locus_tag="VGR29_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEV2074096.1"
                     /translation="MESHRFDALTRNLATDTSRRRILARLTASAAAGILAHLAPASLQ
                     AQGKPDKPDKPDKRDKPDKRDTPGQGPACREAGHPCEGNQECCPSLECVASGPGAARR
                     CTAVAAEECPTCSTEQVAVTALVSYWIDVACAYDAGADLTTCDCTAAGDASAPAATQV
                     VLPPADVCAEVVGGAAEFLAPAPDQRVAGYASQPGEATLTLKLAGEVEAGGTTTYWVT
                     TETGLVPAEGPGLVRIVHDAIGSVEVRAAACDVAAPEDRAGYDWYGRCQQPAEGLTFD
                     LAPVGDEAQGLEGTTDTSGRLRFGSVAQACTTSRSLTVPGATPNRTRSTPAGRWSSRP
                     IGRPRCGFLPAPGSSQDTNWVGRANSGSHHVVPRWRARHFVGAGAICCAYNDGSGMSA
                     YVLLLVAIVVEVVATSALKASDGFTRLLPSIVVVVGYAVTFYLLTLVLRRFALGFVYA
                     TWSGLGTVGVAVIGVLLFGDAMTVTRVVGIAMVIIGVVILNIGGGRRPTPLR"
     gene            complement(1992..2660)
                     /locus_tag="VGR29_10700"
     CDS             complement(1992..2660)
                     /locus_tag="VGR29_10700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV2074097.1"
                     /translation="MSQEQWTAVDRHFSHILHTGDVVLDAALAASDAAGLPQINVTSS
                     QGKLLMLMARMIGANRILEIGTLGGYSTIWLGRGVAGDGRIVTLELVQKHADVARRNL
                     ERAGLDAITEIRVGKATDSLHQLIAEGTEPFDLVFIDADKASLPDYLEGSLRLSRPGT
                     VIIADNVVRSGKVANPDTTDEDVRGVQRYLELVGADPRLESTAIQTVGAKGYDGFAIS
                     FVVG"
     gene            complement(2820..3197)
                     /locus_tag="VGR29_10705"
     CDS             complement(2820..3197)
                     /locus_tag="VGR29_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074098.1"
                     /translation="MMQRTIGMYIANKIKDEVFAPQKVETEDGEVEYIEPAHDPLDGE
                     LIRDYEGRALGRAYRVPDVAVYVVSGNNADPDAELERALNDPQAETIIHWEGDRVAAL
                     VYVSEQARSNAATGVRGVRADDV"
     gene            complement(3229..3993)
                     /locus_tag="VGR29_10710"
     CDS             complement(3229..3993)
                     /locus_tag="VGR29_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEV2074099.1"
                     /translation="MPDEHDRTPKPYGDPATTTPERVLLIQAHPDDAEFSCAGTIANW
                     AANGAEIHYCSITSGDKGSDDAEMTGVALANMREEEQREACRILGVKSVTFLGYPDAM
                     LMPDLNLRRELVRVMRRIRPTTLVCQDPTMRFAGREYINHPDHIAAGEASLAAVFPST
                     RDRMTFPELLQEGLKPHKVPNIYIYGAAEPDCWVDITTSIETKIAALKAHASQLGDWD
                     PTEMIQEWGRRSAYANPMKPEGTGQYAEAFKYMHLR"
     gene            4210..4707
                     /locus_tag="VGR29_10715"
     CDS             4210..4707
                     /locus_tag="VGR29_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV2074100.1"
                     /translation="MRASNHGLMQPREKLDPRAKRVWMLGDLFGAVVFGLVAFAIAFV
                     ARWVFELHWAWVVVATGVVLALSLTWAIVSPSIRYEQWRFEIREDEVDLLHGLVVRTR
                     QIVPMSRIQHVDTTRGPIQRRYGLASVVFYTAAGGMTIPELSDERAASVRDQIAALAK
                     VHDDL"
     gene            4697..6271
                     /locus_tag="VGR29_10720"
     CDS             4697..6271
                     /locus_tag="VGR29_10720"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV2074101.1"
                     /translation="MISDPGLAPAQDSPDAVAWQRAHPAFMVTALLRNLRGFILPFAL
                     VAIGNSGRESRTGTLIFLGISAVVILLSGIGSVVQWWFYRYALMPDRLLVHSGIIFKQ
                     ERAIPYQRIQTVDLEEAPLDRLLGVARMKIETAAGGGGESEVELKAVRRGAALALREQ
                     LLMARQTVQGDVAPEPGATGQDDETPATRVMESSGELVRKLSLGELLLAGATSGTIGP
                     AAAIIGVGIQFADDIVPQAWWNRVPWDQVTGASRNLSVIAGVVIVIAVFAWLVAIAGT
                     VLTYYGFELRRDGEHLVVQYGLLDRRRTTIPIRRIQAIRIEEGLLRQPFGYAAVRYES
                     AGRAGAAEGGSGTLFPMMPRNAVRDLLAITAPEFAVDVDRPQFQRLPRRSLPRYIVAD
                     SISSLLVVVLIMVGIRFWQDGLPWWGYLPLLWVPIVVVLGWLSFRDAGWKLDERVLML
                     RTRGAARSTLVTPRRRIQYRQTTANPLQRRADLVTLQIAVASGGFGGHYALRHIDAAD
                     GRTLLLALNPRLTSAR"
     gene            complement(6402..7748)
                     /locus_tag="VGR29_10725"
     CDS             complement(6402..7748)
                     /locus_tag="VGR29_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074102.1"
                     /translation="MRFTSRFSRMTGLLVLLLTMLAMLVTSPAASAQSGDSSIEVHNR
                     ICPQNYQGQNYFEDCHDNPPDPGLPFTFTDGVTREGTTNENGNIGFANLPAGTYTITG
                     GAPGAYTNYVVYCAVGTEAESNQQQIPVEYVTGGIQFDLPASTNVICDWYTIPLSQNG
                     EPAPTPTEAAAPDTFDLPIYKLLCEDDPGTTAASDFVMMGTLPAGCEQFAGVTVTVAA
                     ADGTVLGSCVTQATEPCYVNVPIGATVYATEDLTAVPEGYVPLNGETIEQEIPAFSEA
                     WTLFINVRSQPPTPVPTATQVVPTATAIPLEPGQLLQIHEGACSESEEELGTVLFTLT
                     DLRAPVQEENVTTDVVVAETSTSTVPVTLDELIVSDNTIVAYGEGDDPSPVACTEIAG
                     PVNDNGELVLGLKEVDNSNYTGIVYLAPSADGTQTDISVFLAEGLAEENLPATPGA"
     gene            complement(8083..8646)
                     /locus_tag="VGR29_10730"
     CDS             complement(8083..8646)
                     /locus_tag="VGR29_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074103.1"
                     /translation="MPASFPRLRPLLIAIVIVILGAMITAPGSTEARETRTIVDTWTL
                     EIGFVEEPPIQSDTNGLWVKVTQEDQPVEGLEATLSAQAIFAGQARELPLTPVAGQPG
                     VYTSVFIPTQPGEYSLLLKGTIGDQPIEETFNASPEGVPLVASRLDYEFPTAANGIVS
                     NAAYPAAIGGALLLAGLIGLVARRNRH"
     gene            complement(8837..10102)
                     /locus_tag="VGR29_10735"
     CDS             complement(8837..10102)
                     /locus_tag="VGR29_10735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEV2074104.1"
                     /translation="MAVKVFSVASMGREASRTRQRSRHIGAGTLALILAVLSLMLSSL
                     MPAAQQAAAQSSDWSPPSTVYIPETGHSLDRLFLDLWRNAGGASAFGYPISPEITTED
                     GHVVQYLQFARFEYWPEGDENGNTVILGKIGKELRPITVQRSLIASTQPGNSAVLLDS
                     ARQMKAWLPVDAASVDMGRNDLVYVEATQHTIWGGFLTFWQNSGAESYLGNPLTEEYT
                     LNGTTYQVFERGQLTWKPGSAISLVPVGKMLAEKYGVSTEPIAQGTLPTYSENLFVPP
                     PEPTPEPTVEIAKVNEEFADVPSGEVWIDISLSYEYMTVYQGNTVLMETYISSGKPGF
                     ETPAGTYFINSKIPEQDMEGVLGGEYYNVPSVPSVMYFTDVGHAIHGAYWHNNFGTPM
                     SHGCINVPVDLAAYLYNIASVGTRVEIHW"
     gene            complement(10120..10377)
                     /locus_tag="VGR29_10740"
     CDS             complement(10120..10377)
                     /locus_tag="VGR29_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074105.1"
                     /translation="MSAAWLHNHGEGETVPLLHAFIEGRDRTKEPGGVRLAPFAGSAI
                     SGTATNFSTPGDVTGKGMGYVRTRELRLARAENLDLEDGSL"
BASE COUNT         1924 a         3219 c         3312 g         2079 t
ORIGIN      
        1 gatgcccatc agacggtgcg gaccaactgc gacgaggact atctgaactg atcgtagcgc
       61 gcacttctca ccacgcaacg gacgcacacg acgcggctcc tgctccctgc gggggccgtt
      121 gtcgtgtccg gtggttggca cagaacagac gccgttgctg gagccgccgg cgcctgtctg
      181 tgtcccaggg agggacacgt cttgccctgc agtgcacatg ccaacgcccc tcggctgggg
      241 agtcaggggc gttggcggga caggaatgga acattctttc accggagcgg ggtcggtcta
      301 cgccccccac caatgttgag aataaccacc ccgataatca ccatcgctat tcccacgacg
      361 cgcgtgactg tcatcgcgtc accgaacaac agcacgccaa tgacagcgac cccaacggtg
      421 cccagtcccg accaggtggc gtagacgaag ccgagggcaa accgccgcag gaccagggtc
      481 agcaggtaga acgtgactgc atagccgaca acgaccacga tactcggcag gagccgcgtg
      541 aacccgtcgg atgccttgag ggccgaggta gccaccacct ccacgacgat ggcgaccaag
      601 agcaggacat aggcactcat tccacttcca tcattgtatg cacagcatat cgcacccgcg
      661 cccacgaagt ggcgggctcg ccatcgagga actacgtgat gtgacccact gttcgcgcgt
      721 ccaacccagt tggtgtcttg gctactgccc ggcgcaggta aaaatccaca ccgtggacgt
      781 ccgattggcc tcgacgacca cctccccgct ggcgttgacc ttgtccgatt cggcgtggca
      841 ccaggcaccg tcaacgaacg tgaggtggta catgcctgtg ccacgctgcc gaaccgaagc
      901 cggccgctgg tgtccgtcgt cccctcgagc ccctgtgcct cgtcacccac tggagcaagg
      961 tcgaatgtga ggccctccgc aggctgctgg cagcggccgt accagtcata cccggcgcgg
     1021 tcctcgggag cggcaacgtc gcaggcggcg gcacggacct ccacgctgcc gatggcatcg
     1081 tggacgatcc gcacgagacc gggtccttcg gcgggcacca gaccggtctc ggtggtgacc
     1141 caataggtcg tggtcccgcc tgcctcgacc tcgcctgcga gcttcagggt caatgtagcc
     1201 tcccccggtt gcgaggcata gccagccacg cgctggtccg gcgccggtgc caggaactcc
     1261 gctgccccgc cgaccacctc ggcacagaca tcggctggag gcagtacgac ttgggtggct
     1321 gcgggggcac tggcgtcacc agcggcggtg cagtcgcagg tcgtcagatc ggccccggcg
     1381 tcgtaggcgc acgcgacatc gatccagtag gagacgagcg cggtgacggc cacctgctcg
     1441 gtcgagcacg tgggacactc ctccgcagct acagcagtgc agcggcgtgc cgcccccgga
     1501 ccgctggcga cgcactccaa gctcgggcag cattcctggt tcccctcgca ggggtggccg
     1561 gcctctcggc aggcgggacc ctgccccggc gtgtcacgct tatcgggttt gtcacgctta
     1621 tcgggtttgt caggcttgtc gggtttgccc tgggcctgaa gtgacgctgg tgcaaggtga
     1681 gccaggatgc ccgcggcggc actcgcagtg agacgagcaa ggatgcggcg gcgcgaggtg
     1741 tccgtggcca ggttccgggt aagcgcatcg aaacggtggg actccatggc gacagctccc
     1801 tcagtcggcg acggtagaca agtacgaggg gtcaggtaca ggtgacgcga acacagcact
     1861 ccctgtgcca tgagggaaga ggtctgtacg ttgaaggagg acactgatag aagatcagca
     1921 gatgcgcgac gaagtcattg gtcgtgatcg agatcagcac caaccgctga tcatcttgct
     1981 tgacctttgc cttaccccac cacgaaagag atcgcgaatc catcgtagcc cttggcaccc
     2041 acagtctgga tggccgtgct ttccaatcgc ggatcagcac cgaccaactc gagataccgc
     2101 tggacgccac gaacgtcctc atccgtggtg tccgggtttg caaccttgcc gcttcgcacc
     2161 acgttgtccg caatgatcac ggttccggga cgcgacagcc ggaggctccc ttccagatag
     2221 tccggcaggc ttgccttgtc ggcatcgatg aagaccaggt cgaacggctc tgtcccttcg
     2281 gcgatgagtt ggtggagaga gtcggtggcc ttgccgaccc ggatctccgt gattgcgtcc
     2341 aggccggcgc gctcgaggtt tctccgcgca acatccgcat gcttctgcac cagctccagc
     2401 gtcacgatcc ggccatctcc tgccaccccc cgcccgagcc agatggtgct gtagccgccg
     2461 agcgtgccga tctcgagaat acggtttgca ccgatcatcc tggccatcag catcagcaac
     2521 ttgccttggc tcgatgtcac attgatctgc ggtagtccgg ccgcgtcgct tgcggccagc
     2581 gcggcatcaa ggacaacatc cccggtatgc aagatatgac tgaagtgcct gtcgaccgcg
     2641 gtccactgtt cctggctcat cgccgtctcc tgtaatgagt cctgcgggca gggaatacgt
     2701 cccggagtcc gggctgccgc actcccggtg acgcgtttcg gctcccaggt caccgggaag
     2761 gcaagtgtcc gggatgcagc accacagcgg acatacggtc cgcatgaatc ttgaccgccc
     2821 tacacgtcgt cggcgcggac gccgcggaca cccgtcgcgg cgttggagcg cgcctgctcg
     2881 gatacgtaga caagcgccgc cacacgatca ccctcccagt ggatgatcgt ctcagcctgc
     2941 gggtcgttca gtgcccgctc cagctcggca tccggatccg cgttgtttcc ggagacgaca
     3001 tagacggcaa catctggcac gcgataggca cggccgagcg cccgaccctc gtagtcacgt
     3061 atcagctccc cgtcgagagg gtcatgggcg ggctcgatgt actcgacctc gccatcctct
     3121 gtctccactt tctgaggggc gaagacctcg tccttgatct tgttcgcgat gtacattccg
     3181 atcgttcgtt gcatcatggt gagcctcctg tcggtggtga atgtagtgct aacgcaggtg
     3241 catgtacttg aacgcttcgg cgtattggcc ggtcccttcc ggcttcatcg ggttcgcata
     3301 ggccgatctc cggccccatt cctggatcat ctccgttggg tcccaatcgc cgagttggct
     3361 ggcgtgtgcc ttcagcgcgg cgatcttcgt ctcgatcgaa gtggtgatgt cgacccagca
     3421 gtccggttcg gccgcaccat aaatatagat gttcgggacc ttgtgcggct taagcccttc
     3481 ctgcaacagc tccgggaagg tcatgcgatc gcgcgtcgag gggaagacgg ccgccaggga
     3541 cgcctcaccg gctgcgatgt gatccgggtg attgatgtac tcccggccgg caaagcgcat
     3601 ggtgggatcc tggcagacga gcgtggtcgg ccggatgcgc cgcatgaccc gcacaagctc
     3661 gcggcgaaga ttcaggtccg gcatcagcat ggcgtccgga tagccgagaa aggtgaccga
     3721 tttcacgccc aggatcctgc aggcctcgcg ttgctcctcc tcgcgcatat ttgcgagggc
     3781 gacgccggtc atctcggcat cgtcgctgcc cttgtcgccg ctggtgatgg agcagtaatg
     3841 gatctccgcg ccattggccg cccaatttgc gatcgttccc gcgcacgaga actctgcatc
     3901 gtccgggtgg gcctggatga ggaggacgcg ctccggcgtc gtggtagcgg gatcgccgta
     3961 gggcttcggt gtgcggtcgt gttcgtcggg catggttctc gctctcttgt aggctgcaaa
     4021 catgacacac ttcagtgtca gtgacgtcgc gcgggtcgca ctgccagatg gcaccatggc
     4081 cggtgaaacc tgccggtact tctgtccctt gcgagtatcg caagaagacg gctcgcgcgc
     4141 atgcccggtc gtatagggag atcaatgcag ccattagggg aaactaccgt accaacgccc
     4201 gatgctgcag tgagagcatc caatcacggg ctgatgcagc cacgggagaa gctggatccg
     4261 cgcgccaagc gggtatggat gcttggtgat ctctttggag cagtggtctt cggcctcgtg
     4321 gcctttgcga tcgcctttgt tgcgcgctgg gtctttgagc tccactgggc ctgggttgta
     4381 gttgctactg gcgtagtgct tgcgctcagt ctcacctggg cgattgtctc accctcgatc
     4441 cgctacgagc agtggcggtt tgagatccgt gaggatgagg tcgacctgct ccatggcctt
     4501 gttgtcagga cccgtcagat cgtgccaatg tcccgaatcc agcatgttga tacgacacgc
     4561 ggtcccatcc agcggcgata cggactcgcg agcgtcgtct tctacacagc cgcgggaggg
     4621 atgacgatcc cggagctctc cgacgagcgc gcggccagcg tgcgcgacca gatcgccgca
     4681 ttggcaaagg tgcacgatga tctctgatcc aggactcgca ccggcccagg acagcccgga
     4741 cgcggtcgca tggcagcggg cgcatcctgc cttcatggtg accgcactcc tgcgcaatct
     4801 tcgcgggttc atcctgccct tcgccctcgt cgctatcggg aacagcgggc gcgagagcag
     4861 gaccggaacg ctcatcttcc tgggcatctc ggcggtggtg atcctgctct ccggaatcgg
     4921 cagtgtcgtc cagtggtggt tctaccggta tgcgttgatg ccagaccggc tcctcgtgca
     4981 ttccgggatt atcttcaagc aggaacgggc aattccgtat caacgcatcc agacggtcga
     5041 tctcgaggag gcgcccctgg atcgacttct gggcgtcgcc cggatgaaga tcgagaccgc
     5101 ggcgggcggt ggtggcgaga gcgaggtcga gctcaaggct gtccggcggg gggcggcgct
     5161 cgcgctacgg gagcagttgc tcatggcgcg gcagacagtt cagggtgatg tcgcaccgga
     5221 acccggcgct acgggccagg atgatgaaac acctgccaca cgggtaatgg agtcatcagg
     5281 cgaacttgtc cggaagctgt cactgggcga gttgctcctc gccggtgcca catcggggac
     5341 gatcggccct gcggcggcaa tcatcggtgt ggggattcag tttgccgatg atatcgtgcc
     5401 ccaggcctgg tggaaccgcg tcccttggga tcaggtgacc ggtgcctcaa ggaatctcag
     5461 cgtgattgcc ggggttgtca ttgtcatcgc cgtctttgcc tggctggttg cgatcgctgg
     5521 aaccgtgctg acctactacg gctttgagct gcgtcgcgat ggtgagcacc tggtcgtgca
     5581 gtatgggttg ctggaccggc gccggacgac gatcccgatc cgccgcatcc aggcgatccg
     5641 gatcgaggaa ggactgctgc gccagccgtt cggatatgcg gcggtgagat acgaaagtgc
     5701 cgggcgggcg ggagcggccg aagggggctc tggcacgctc ttcccgatga tgccgaggaa
     5761 cgcggtgcgg gatctgcttg ccatcacggc acccgagttc gctgtcgatg tcgacagacc
     5821 gcagtttcag cggctgccgc ggcgatcgct gccccggtac atcgtcgcgg actcgatctc
     5881 ctcgctgctc gtggtcgtcc tgatcatggt cggcatccgg ttctggcagg acggccttcc
     5941 ctggtgggga tatctgccgc ttctgtgggt cccgattgtg gtcgtgttgg gatggctctc
     6001 gtttcgggac gctggctgga agttggacga gagagtgctg atgctgcgca cgcgcggggc
     6061 ggctcgctcc acccttgtta ccccgcgacg gcggattcag tatcgccaga caaccgcgaa
     6121 tccactccag cgccgggctg atctcgtcac gcttcagatt gccgttgcca gtggcgggtt
     6181 cgggggacat tacgcccttc ggcacatcga cgcggcggac ggccgcacgc tcctcttggc
     6241 gctcaatcca cggctgacgt cggcgcgata gagttcgggt cgcagtgccg atcgccaccg
     6301 tctagggcag atcatcatcc acctcgcgat tagctcggaa ggggtcggag ctctgtattg
     6361 ctccggcccc ttcgttcaca ccaatggtgc acggattgtt gctacgcgcc cggtgtcgcc
     6421 gggaggtttt cctcggccag gccttccgcc aggaagaccg aaatatcggt ctgggtgccg
     6481 tcagcggacg gcgcaagata gacgatacca gtgtaattgg aattatccac ctccttgaga
     6541 ccaagcacca gttcgccgtt gtcgttcact ggacctgcga tctcggtaca tgccacaggg
     6601 gagggatcat ccccttcgcc ataggcgacg atcgtgttgt cggacactat cagctcgtca
     6661 agtgtcaccg gcacggtgga ggtggaggtt tcggcgacca ccacatccgt ggtgacgttc
     6721 tcctcctgaa ccggagctcg caggtcggtc agcgtaaaca ggactgtgcc gagttcctct
     6781 tcagactcag aacatgcacc ctcatgaatc tgcagcaact ggcctggctc aagtggtatc
     6841 gccgttgccg tcggcaccac ctgcgttgct gttggcactg gcgttggtgg ctgtgaccgg
     6901 acattgatga agagcgtcca ggcttcagag aacgccggga tctcctgctc gatggtctcg
     6961 ccgttcagcg gtacgtagcc ctccggtact gctgtcaggt cttcggtagc atagacagtt
     7021 gctccgatgg gcacgttcac gtagcaaggt tctgtcgctt gggtaacgca actgccgaga
     7081 actgtgccgt ctgccgcagc gacggtaacc gtgacaccag cgaactgctc gcagccggcc
     7141 ggcagggtgc ccatcatcac gaagtcgcta gctgccgtag taccaggatc gtcctcgcag
     7201 agcagtttgt agatcgggag atcgaatgta tcgggcgccg cagcttcagt gggtgtcggt
     7261 gcgggctctc cattctggct cagcgggatg gtgtaccagt cacagatgac gttggtactt
     7321 gctggtagat cgaactggat acctcccgtc acatattcga cgggtatttg ctgctggttc
     7381 gactcagctt ctgtaccaac cgcacagtac acgacgtagt ttgtataagc acctggggcg
     7441 ccacccgtga tggtgtaggt gcccgcaggg agattagcga agccgatgtt gccgttctca
     7501 ttcgtcgtgc cttcccgggt cacgccatca gtaaacgtga agggcaatcc cggatcgggc
     7561 gggttatcgt gacaatcctc gaagtagttc tgaccttgat agttctgggg gcaaattcgg
     7621 ttgtgaacct cgatcgaact atcacctgat tgtgcagatg cggcgggcga agtgaccagc
     7681 atggcgagca tggtgaggag aagcaccagc agaccggtca ttcgactgaa acgtgacgtg
     7741 aagcgcatct ctgcaacctc caatcctctg ggatgagtcc ccggagcagg atggtaatca
     7801 gcacaatagt gccaatgcag ggactcgtcc tcggctacag gcgaagcggt tttcgcccgc
     7861 gaacaaagag acaccgtgac agatcaattg cactgggtag tctcttccga cggtgttgca
     7921 gcaagatcgg ttcctcactg ccgaggaact gcaggagtct cgagagttcc acccactgaa
     7981 ccttgggcag gagcaataac accggacagc ggctgaacct tactcattgg tcaaccggta
     8041 tccgggattg gattgctatc acggtaccgc cggaatttaa ggtcaatgcc ggttgcgtcg
     8101 cgccacgagc ccgatcaggc cggcaagcaa cagcgccccg ccgatggctg cgggataggc
     8161 agcgttcgag acaatgccat tcgcggcggt cgggaactcg tagtccagac gcgaggccac
     8221 caggggcacg ccctctggtg aggcgttgaa tgtctcctcg atgggctggt cgccgatcgt
     8281 tcccttgaga agcaagctgt actcgcctgg ctgcgtcgga atgaagacag acgtgtagac
     8341 gcctggctgg cctgccaccg gagtcaacgg caattcccgc gcctgaccgg caaagatcgc
     8401 ctgggcggag agcgttgcct ccaggccctc aacgggctgg tcttcttgag tcaccttcac
     8461 ccacagtccg ttggtgtcac tctggatcgg tggctcctcg acaaatccga tctcaagcgt
     8521 ccatgtgtcg acgatcgtgc gggtttcccg ggcctccgta gaacccgggg cggttatcat
     8581 ggcgccaagt atcacaatga caatggcgat cagcaggggc cgcaggcgcg ggaatgaagc
     8641 gggcatgtga gcgtctcctt gcggtaaaaa ctgggataat cgagccgttc ccggtccttc
     8701 gaaaagccta ccatgcacaa tggccgctcc cggaagatga ccggcagggg agccggggct
     8761 ggagaggcgc tgaacacaag aagcccacga gagtgcatcg ctctcgtggg cttcttcgat
     8821 caactcgggc gggaggttac cagtgaatct ccacccgggt gccgaccgat gcgatgttgt
     8881 agaggtaggc tgccaggtca accggtacat tgatgcagcc gtggctcatc ggcgtgccga
     8941 agttgttgtg ccagtatgca ccgtggatcg cgtgtccaac atcggtgaaa tacatcaccg
     9001 agggaacaga cggcacgttg tagtactcac cacccagcac gccttccatg tcctgctcgg
     9061 gaatcttgga gttgatgaag tacgtgccgg caggggtttc gaagcccggc ttgccagagc
     9121 tgatgtaggt ctccatcagc acagtgttgc cctggtagac cgtcatgtat tcgtaggaga
     9181 ggctgatgtc gatccagact tcgccggacg gaacatccgc aaactcctcg ttcaccttgg
     9241 caatctcaac ggtcggctct ggtgtcggtt caggtggcgg aacgaagaga ttctcggaat
     9301 aggtcgggag ggtgccctgg gcgatgggtt cggtgctgac gccgtacttc tccgccagca
     9361 tctttccgac cggcaccagg ctgatggcgc tgcccggctt ccatgtcagc tgaccacgct
     9421 cgaagacctg ataggtcgtg ccattgagcg tgtactcctc ggtgagaggg ttgccgagat
     9481 agctctcagc accactgttc tgccagaagg tgaggaagcc gccccagatc gtgtgctggg
     9541 ttgcctcgac gtagacgaga tcattgcgtc ccatgtcgac cgaagcggca tcgaccggga
     9601 gccacgcctt catctggcgt gcggagtcca gcagcacagc gctgttgccc ggttgtgtcg
     9661 atgcgatgag cgagcgctgc acggtgatcg gccgcaactc cttgccgatc ttgccgagga
     9721 tcacggtgtt gccgttctca tcaccttccg gccagtactc gaagcgtgcg aactggaggt
     9781 actggacaac atgtccgtcc tcggtagtga tctcaggtga gatgggatag ccgaaggcgg
     9841 acgccccacc agcgttacgc cacaggtcga ggaagaggcg gtccagggaa tggcccgttt
     9901 ccggaatata gacagtcgac ggcggtgacc agtcagatga ctgcgcggca gcctgctggg
     9961 ctgccggcat gaggctggaa agcatcaggc tgagaaccgc aagaatcaat gccagcgttc
    10021 ccgcgccaat atgcctcgac cgttgccggg tgcgagatgc ctcacgaccc atcgacgcta
    10081 cagagaaaac cttcaccgcc actccccttc gctctttcat cataacgagc cgtcttcgag
    10141 atccaggttc tccgcccgag cgagacggag ttcccttgtc cggacgtatc ccatgccctt
    10201 gccggtcaca tcgccggggg tagagaagtt cgtcgccgtt cccgaaatgg cacttcccgc
    10261 aaacggtgcc aggcgcacgc cgcctggctc tttggtgcgg tccctaccct cgataaacgc
    10321 atggaggaga ggaacagttt ctccctctcc atggttatgc aaccatgccg ctgacacgca
    10381 ccgcccctga caggcagggc atcgccgtgc cttcatatag gtgtacgtac actatacgtc
    10441 accgggttcc ctatgggaag agggtttcga ccggtcggga tcgaccgatg ccaatgggca
    10501 ccataccacg actgggaggg cgctgtccca tggg
//
LOCUS       DASYWM010000264         4482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_631283, whole genome shotgun sequence.
ACCESSION   DASYWM010000264 DASYWM010000000
VERSION     DASYWM010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631283"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VGR29_10745"
     CDS             complement(<1..234)
                     /locus_tag="VGR29_10745"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEV2074106.1"
                     /translation="MTELSDIALMERITLRHTDALRGLYDRYGRIAFALAYRVVGEAS
                     AAEEVVQDAFETVWNKGHSFDASKGGNVRGWLLT"
     gene            548..1159
                     /locus_tag="VGR29_10750"
     CDS             548..1159
                     /locus_tag="VGR29_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074107.1"
                     /translation="MQQSLQVHPHTLGSPSVPGSVVTVPAGDEARPALWPVALPNGSG
                     SRTDASVHQTTNVNVNVGMPSIHFNTAKAGPSFFVRALWFLAIGWWLSAFFIVAGYVL
                     VASVFLLPMGLWMLNRIPQAQTLRPRTREFTTQFRDNAIAFTEGRKQQIAWYLRLLYL
                     PAGLVLGAAWLVAAWALSLTVIGLPLSVMMVDRAPAIITLQQN"
     gene            1327..1566
                     /locus_tag="VGR29_10755"
     CDS             1327..1566
                     /locus_tag="VGR29_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074108.1"
                     /translation="MEAMTSPTDNSATYSLWHIPTSTLLVMTNAVAEVMRRIELATTE
                     GVFLAELMLNVDQDGSLVGKQHLGPCMLDALPLHE"
     gene            1977..2375
                     /locus_tag="VGR29_10760"
     CDS             1977..2375
                     /locus_tag="VGR29_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074109.1"
                     /translation="MFRRKEMISPESRSARKRAKALGLASYGLGIMQITRPGSMNRFI
                     GVPDYNPNHTLQRILGAREIMSGTGILFGENTSGWMWSRVAGDVMDAGIIGGVLASGL
                     GMPKRLVPALVAVAGLMVLDAKVAMRTGDR"
     gene            2504..2923
                     /locus_tag="VGR29_10765"
     CDS             2504..2923
                     /locus_tag="VGR29_10765"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV2074110.1"
                     /translation="MRLIQTSWPVVILRCLPALLCMAVIFELSSRSELPKVSSISDEA
                     ISLGGHFGAYAILAVCFWWALGMTDLASDKRVLVALAGAVLYGLTDEWHQSFVPGRTP
                     DIRDIFVDGIGAMAGLFLVTLLARRLEADNQERENYR"
     gene            2920..3810
                     /locus_tag="VGR29_10770"
     CDS             2920..3810
                     /locus_tag="VGR29_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV2074111.1"
                     /translation="MSGKLYPDLPEVGPLELGKRVFREFRSDDMPLYAAALSYHSLLA
                     IFPFLIFLLGLLSFLRIPGFFEWVIEQGNVALPPDAARVVNDVVAGIQEGGNGGLLSF
                     GALFALGAASTGVRALMSALNTAYDIAETRAWWKKYPLSVIYTLGLAVLLIIASAGFV
                     LGPQTAQWLSGKVGLGDAFVMIWAWLRFPVVVVILMLVAALVYYVVPNVDQPFRFITP
                     GAMIAVLIWILASIGFSVYVNNFSTYNATYGSLGGIVILLLYFFISAAVLLLGAEINA
                     EIYQLKRGHAEPQDTSGVEE"
     gene            complement(4006..>4482)
                     /locus_tag="VGR29_10775"
     CDS             complement(4006..>4482)
                     /locus_tag="VGR29_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074112.1"
                     /translation="YQYQRGSQVIRVLEMYSVENGTWTLRQQQSESPETDEDTITISV
                     KIGRDPALEISPTSFAETPATRLTIQSSASSDLGVALFSASEEVDTEALVGTAVDAVP
                     ETLTLEGETSVVAGTTDEMLFEGLKEGNYVVAVLDGSGTVTAASTLTVGPPADLGL"
BASE COUNT          814 a         1342 c         1385 g          941 t
ORIGIN      
        1 cgtcaggagc cagccgcgaa cattgccacc cttcgacgca tcgaacgagt gccccttgtt
       61 ccagacggtt tcaaaggcat cctgcaccac ctcctcggca gcggatgcct cgccgaccac
      121 ccggtacgcg agcgcaaagg caatgcggcc ataccggtca tagagtcccc tgagcgcatc
      181 cgtatggcgg agggtgatgc gctccatgag cgcaatgtcg ctcagctcgg tcaccagggt
      241 gtgcagcctt tcgcctgaca gttgaacgtc agcagcatcg gatttggatc attctcaggg
      301 ggcattttcg cacattgcct gccctttcgc attgccggga agactcagta attcccgcaa
      361 tccgcgaatc ggtacattta cggatggcgt ctgaacctgc tgatggcatg ctggttctac
      421 accggattgg tgatgggggg acgggcaaga ccaggagtca cctggcgatt gccctgacga
      481 gaacatcaca ccgtcacgtc cggaacgcgc cgcccggcgt ttgggcaatt acaggaaggg
      541 gttcccagtg cagcagtcgc ttcaggtcca tccgcatacg ctcggctcgc cgtcagttcc
      601 cggttcagtg gtcacggttc ccgctggaga tgaggcccgc ccggccctct ggcccgtcgc
      661 gcttcccaac gggtcaggga gcaggaccga tgcatcggtc catcagacaa ccaacgtcaa
      721 tgtgaacgtc ggcatgccgt cgatccactt caatacggcg aaagcagggc cgtcgttctt
      781 tgtccgggca ctatggttcc tggcgattgg ttggtggctt tccgcattct ttatcgtcgc
      841 tggctatgtt cttgtcgcgt cggtcttcct gctaccaatg ggactatgga tgctgaaccg
      901 cattccccag gcgcagaccc ttcgtcctcg tacccgcgaa ttcaccaccc agtttcgtga
      961 taatgcgatc gccttcaccg agggccggaa gcagcagatc gcgtggtacc tgcgtcttct
     1021 ctatcttccg gccggcctgg ttctcggcgc agcgtggctc gtcgcggcat gggctctcag
     1081 cctgaccgtt atcggcttgc cgctctcggt catgatggtt gaccgtgccc cggcaatcat
     1141 caccctgcag cagaactgaa aatgggggat accgtgtaac agcatggtcg ttcccactga
     1201 tattcagacg ggacgggtcg tgacgaaccc aagttagccc agagcccatg attacttagg
     1261 tggggaagac gaaaaatcag taattttacg gatgccgggg agtcgtggaa ctgtcatgat
     1321 agcagtatgg aagcgatgac gagccccacc gataacagcg ccacttactc cctgtggcat
     1381 atccccacca gcaccctgct ggtgatgacc aatgccgtcg cggaggtgat gcgccggatc
     1441 gaactggcca cgaccgaagg tgtgttcctg gctgagctga tgctcaatgt cgaccaggac
     1501 ggctcgctcg tgggcaagca acacctgggg ccgtgcatgc tcgacgcgct tcctctccat
     1561 gagtagtgtg ttcccccatc acattacttc ctttcgaaga caccgccgcc cagccacggc
     1621 ggtgtcttct tgtgtgaagc gtcggtgcct ggccacagcg tcaaggtgat ggccgaaccg
     1681 gggccgcatg cgcaagtaat tgcccgaagc tggatcttcc catgggaagc gccggacgcg
     1741 gcgcttgatg agagcggcat ccatctcata ttgcggcatg gacggtgttc tggccctcgg
     1801 ccggatctgg agcagcacag cgtacaacgg catgaatact gcatcccgga tcgaggccgg
     1861 tgggtaccac tggcggcagt gattcgaatg agggcaggct ggttgtatgg ccgctcccag
     1921 ccgagcgggg tccaccggcc caacgatgca gcaccacact cgtcaggaga agagagatgt
     1981 tccgacgcaa ggagatgatt tcaccagaat ccaggtccgc ccggaagcgc gccaaggcgc
     2041 tcggcctcgc aagttatggc ctgggcatta tgcagataac ccgaccggga agcatgaacc
     2101 ggtttatcgg cgtccccgat tacaacccca accacacctt gcagcgaata ctcggcgcgc
     2161 gggagatcat gagcggcacc ggcatcctgt tcggggagaa taccagcgga tggatgtggt
     2221 cgcgcgtggc aggtgacgtc atggacgccg gcatcatcgg tggggtgctg gcatcggggc
     2281 tcggaatgcc aaagcgccta gttccggcgc ttgttgcggt ggcggggctc atggtgctgg
     2341 acgccaaggt ggccatgcga acaggcgatc gttagcacgg cggggaatac accggaatcc
     2401 tggttgcgag accggtcgcg gggcaatatg ggtatgcttg tagatatgca tacgcgtgtt
     2461 gccttgccct tttcgggtcc aatccccggg agtatctctc ttcgtgcgac tgatacagac
     2521 gagctggccc gtcgtcattc tgcggtgcct gccggccctg ctctgcatgg cagtgatctt
     2581 cgagctctcc tcccgcagcg agctgccaaa agtcagcagc atcagtgatg aagctatttc
     2641 gctaggcggg cacttcggtg cttacgccat cctcgccgtc tgtttctggt gggcgctggg
     2701 gatgactgac ctggcgtccg acaaacgcgt cctggtcgcg cttgccggcg cagtgctcta
     2761 tgggctcacc gatgaatggc atcaatcctt tgtgccgggg cgaacacccg atatccgtga
     2821 catcttcgtg gacggaattg gtgccatggc agggctcttc cttgtcaccc ttctcgcgcg
     2881 tcgcctggaa gctgacaacc aggaaaggga gaactaccgg tgagcggaaa actctatccg
     2941 gatctgccgg aggtagggcc acttgaactc ggcaagcgcg tgttccggga gttcaggagc
     3001 gacgatatgc cgttgtatgc ggcagcgctt tcgtatcaca gcttgctggc gatcttcccg
     3061 ttcctgatct tcctgctcgg cctgctgagc ttcttgcgca ttccggggtt cttcgaatgg
     3121 gtaattgagc aggggaacgt ggctctgccg cccgatgccg cgcgcgtggt gaacgatgtg
     3181 gtggcgggca tccaggaggg gggcaacggc gggttgcttt cgttcggcgc gttgttcgct
     3241 ttgggagccg cgtccaccgg cgtgcgggca ctgatgagcg cgctcaatac tgcctacgac
     3301 atagccgaga cacgggcatg gtggaagaag tacccactgt cggtgatcta tacgcttggg
     3361 ctcgcggtgt tgctgatcat cgcaagcgcg ggatttgttc tcgggccgca gacggctcaa
     3421 tggctttcgg gcaaggtagg gttaggcgac gcctttgtca tgatctgggc ctggctccgc
     3481 ttcccggtcg tcgtggtgat cctgatgctt gtggcagcgc tggtctacta tgtggtgccg
     3541 aacgtcgatc agccattccg gttcatcacg ccgggtgcaa tgatcgccgt gctgatctgg
     3601 atcctggcgt cgatcgggtt ttcggtctac gtcaataact tcagcaccta caacgcaaca
     3661 tacgggagcc tgggcggcat cgtgatcctg ctcctctact tctttatctc ggcggcagtg
     3721 ctgctccttg gcgcggagat caacgccgag atctaccagc tgaagcgtgg tcacgcggaa
     3781 ccgcaggaca cgagcggcgt cgaagagtag cgcgacttca aacccgtcgc gccccgggat
     3841 tgtaattgcc atggccaagg gagccctccc cgactctgag gtcacccttc ccgggcattg
     3901 acccgtttgc ttatgctggc ccgggcgatt atccggatgc acaacgccgc cctcccgata
     3961 acccgggagg gcggtgttgt ttcggcccta gagtctaccg aacctctaca ggccgaggtc
     4021 cgcaggcgga ccgaccgtca gggtcgaggc ggcggtcacc gtaccactgc cgtcaagcac
     4081 ggcaacaacg tagttgcctt ccttcaggcc ctcgaagagc atctcgtcgg tcgttccggc
     4141 aaccaccgat gtctctccct cgagcgtcag cgtttctggc acggcatcga cggcggtgcc
     4201 cacgagcgcc tctgtgtcca cttcctcaga ggcggagaag agtgcaacgc ccagatccga
     4261 ggaggcgctg ctctggatgg tgagccgggt cgcgggggtt tccgcaaagc tggtaggcga
     4321 gatctcaagc gccgggtcgc gaccgatctt gacggagatc gtgatcgtgt cctcgtccgt
     4381 ctccggcgac tctgactgct gctggcgcag cgtccatgtg ccgttctcca ccgaatacat
     4441 ctcaagcacg cgaatcactt gcgagccgcg ctggtactgg ta
//
LOCUS       DASYWM010000265        29417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_575353, whole genome shotgun sequence.
ACCESSION   DASYWM010000265 DASYWM010000000
VERSION     DASYWM010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 29417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29417
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575353"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VGR29_10780"
     CDS             <1..500
                     /locus_tag="VGR29_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074113.1"
                     /translation="LLLGMIEEGTGIAITHDTSLTPEQITALRQDNGGLQFNIVQWWL
                     ASSTPSLLATAASRESEYNAGWINWEPDLEPSGEFTPGEDAAAFNDLVDRAEAELDQA
                     IRNDLYYEAETLLLRNAVYIPLGYWVQRFLQKPWLQGTRQGPWTGSTPLRIDEEVVVR
                     GRQQG"
     gene            542..1012
                     /locus_tag="VGR29_10785"
     CDS             542..1012
                     /locus_tag="VGR29_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2074114.1"
                     /translation="MRLPFVISVNFFTVERFSLGGAVRLEPVASWLTVWHRARMPRGY
                     SRDLRERLVQAVASGLSVVEVARRMGVSSSSVHRYKRKVAAGVSLAPGTSPGGPRKIG
                     PTEEEALRAQVLATPDATLAEHCAVWTAGGHSPVSRATMSRALRRLDLPLKKRP"
     gene            1042..1476
                     /locus_tag="VGR29_10790"
                     /pseudo
     CDS             1042..1476
                     /locus_tag="VGR29_10790"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1623..3353)
                     /locus_tag="VGR29_10795"
     CDS             complement(1623..3353)
                     /locus_tag="VGR29_10795"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HEV2074115.1"
                     /translation="MSNGNGAFTFVLHSHLPYARQAGMWPHGEEWIHEAIAETYVPLW
                     TALHDLAEDGVDYRITVSVTPILAEQLADPLIVEHFLTYAEERAAWAAADIERFEEAG
                     DQELMALARHFHHWYSRTLTAFRDRFNGDLLGSLKDLQDRGFVEISTCAATHGYLPLL
                     SRDSSIYGQIQTGVDAYIGHFGRRPKAIWLPECAYRPAYVEEENGVQITRPGLESFLA
                     AQDIRVFFSETHTVEGGRPVGKAAGDAVGIYGAAPRRQLVNQPAEQQQEPGTTYKSYW
                     VGDAPGETAVLGRNNRTGQQVWSGSFGYPGDEWYREFHRKDGVSGMQYWRVGGAEVDL
                     GSKPPYNPEKAQERIGDHARHFVHLVDELLQQYKEETGGYGIIASNYDTELFGHWWFE
                     GVDWLKAVLRGLAELNTVDLTTASTWVDEHGGDEVMILPESSWGMGGNHFTWMNPDTE
                     WMWPAIHGAESRMEALVAEHPDANGELLDVLNQAARELLLLESSDWPFLVTTAQANEY
                     ASQRFNEHVDRFNSLADIVERGTEMTSDERETLAALEHRDNPFANIDYRVFAPRQGSA
                     GLAEDAVHAR"
     gene            complement(3522..5093)
                     /gene="malQ"
                     /locus_tag="VGR29_10800"
     CDS             complement(3522..5093)
                     /gene="malQ"
                     /locus_tag="VGR29_10800"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV2074116.1"
                     /translation="MHFPRESGILLHPTSLPGPHGIGDFGEAAFRFVEWLERAGQRLW
                     QVMPLGPTGFGNSPYSSPSAFAGNPLMVSLAWLQGDGLLTEEELAPAYAFPRHAVDFE
                     QVAPFRMRLLRLAYHRLENGASSNLRDAFEAYRADAPSWLHDYALFVSVKDALGGDSW
                     VDWPKDIRTRQPEAVATWGEKLSDDGRFHEFVQFLFHQQWREVRAYANTRGIQIIGDI
                     PIFVALDSADVWSSQDQFQLDEEGRPTFVAGVPPDFFSSTGQLWGNPTYNWPAMKANG
                     YQWWIDRLRATRDMVDIIRIDHFRGFAAAWHVPFGDENATGGHWEIAPGGEVFAAARR
                     ELGEFPVIVEDLGIITPDVVALREILDLPGMNVLHFAFDDDPENMYLPHNVKRHSVTY
                     TATHDNQTTVGWWNSLPEEERHVVRRYLGRPADDIAWDLLRLAWASVSDTAIAPMQDI
                     LRMGDEGRMNTPATATGNWAWRLLPSQLDPSLADGLRDLTNTYGRMERAVPDAAENPW
                     DYTDPKSGISATKSW"
     gene            complement(5217..6023)
                     /locus_tag="VGR29_10805"
     CDS             complement(5217..6023)
                     /locus_tag="VGR29_10805"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2074117.1"
                     /translation="MNKRMKKGHRYRGMMIALTAVLCLLVTACGIGDTDEGPNETDEP
                     SSAQPTVQGPGTDGSDENNGSPEAPTNIDPSSPGAAGDPLETDDKVRPSPGAGDATPA
                     DSAVPVPGLAASTPEVDGTPAADQPPTGDGTTGAPPPAQAGSSAGTPAAGEAAPAPAA
                     AGGSTPEDGPGTAVADGEIPVVDGCEVEDLPAFTGATSTYRVNSELNFRSGPGTDCDL
                     IGQAPLDVSTVVEVIGGPVTRQGEDGIEWVQVRVGEETGWVALEFLDATE"
     gene            complement(6099..7103)
                     /locus_tag="VGR29_10810"
     CDS             complement(6099..7103)
                     /locus_tag="VGR29_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEV2074118.1"
                     /translation="MPKQVGIDLGTANVLVFVRGRGVVINEPSVVAISAKDGKVKAVG
                     IEARDMLGREPRETIEVVRPMRDGVIADYVVTQKMLEYFINKAVGRFSLIRPDVMICV
                     PAGVTGVERRAVRESALNAGARRAYLISEPLAAAIGARVPVADPSGNMIIDIGGGTTE
                     VAVISLNGIVVARSLRVGGSKFDDAISNHIKRKYNLRVGERTAEEVKIAIGSAMPLDD
                     DKVMEVRGRDEVAGLPRTIQIHASEVTDAITGPLEAIIGAVRAVLEETPPELSSDIID
                     KGMILTGGGAMLRHLPDLLTEVTGVPCYVADDPLSCVAIGTGLALEHMDILRDSLEEP
                     "
     gene            complement(7355..8143)
                     /locus_tag="VGR29_10815"
     CDS             complement(7355..8143)
                     /locus_tag="VGR29_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2074119.1"
                     /translation="MNLDLTKMGFGITSTVDHGVVRELAPRLEEAGFRTLWINHGGSG
                     DSLASIQVAASVTTRIRLASGVIPVDRMVPQEIVRTVQERALPLDRLVLGIGSSAPPS
                     PLNTVSNAASFIHERLGAPVMVGALGPRMRRLGVRETQGVLLNWLTPEAARRALADKD
                     SDLADLPDKRAEVALYIRTALGQDARPALEREVARYEAIPSYAANFARLDFQALDSAV
                     YGETREEIREGLEGFLGTVDEPVVRAITANEALEEFIALVDTVV"
     gene            complement(8166..8861)
                     /gene="radC"
                     /locus_tag="VGR29_10820"
     CDS             complement(8166..8861)
                     /gene="radC"
                     /locus_tag="VGR29_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV2074120.1"
                     /translation="MHEVEQAPYRLMIREMQPDERPRERLRSGGPAALSNSELLAILL
                     NTGIKGESVTTMAQRLLHDCGGLTGLMRMEFVELAGVRGVGESKASKVKAAFELGRRL
                     AAISDQGKPRIESPEDVVQLLGIEMAALEQEQLRVVLLDTKNHIQTSRMVYQGSVNAA
                     SVRAAVRHNSVGIVLVHNHPSGDPTPSAADVNVTLDLVKAGDLLDIKLVDHLIIGQGR
                     HVSLKRLGLGFPK"
     gene            complement(8854..9735)
                     /locus_tag="VGR29_10825"
     CDS             complement(8854..9735)
                     /locus_tag="VGR29_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase subunit LigB"
                     /protein_id="HEV2074121.1"
                     /translation="MPLVAAAIMPHGFALIPALSDDAEGAMETRRAMEEVGRRFADAG
                     VEAVVLAGPHGTRVEGAMCVMDVGRAAGTLARGDRSIEMNVPCDRQLVEAILAGARSA
                     GINAAAAGFGGNRADQSVAPLDWGAVTPLWFVGHDANRPLSGNVLAPNPEKDEGPPVV
                     LITPSRSLSRQKMVDFGQMLGRVFAEDRRKISFIASCDWAHTHREDGPYGAHPKAAEV
                     DAKVVQAVKEHELHTLATLSDEDVTDAAIDGLWQTLMLAGIQDVTPCDAEFLSYEVPS
                     YFGMIVAAYSPTAVPHA"
     gene            complement(9846..11069)
                     /locus_tag="VGR29_10830"
     CDS             complement(9846..11069)
                     /locus_tag="VGR29_10830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2074122.1"
                     /translation="MRQSELVLAVYLPTALLALAQGLLLATLPLYAIDLGASYMLISL
                     ISSAAAIGTLVADVPAGIVLHRIGLRRSMLIGCALVVIGTLGLTLDLTPELIVALRVF
                     AGIGTALWGLSRHSFIAQAIPPERRGSAIALFGGINRVGMFGGPAIGGVIATAFGMQV
                     AFLVAGVLGGCAWVAASMFIPRDAPDQIPKKGASGERWRIVRRTLKENAADLSAAGVA
                     QLFAQMIRQGRQILIPLIGAQQLGLNAAEVGTVMTVSAVIDMSMFFPAGVLMDRFGRK
                     FASVPSFAIMALGIGSVAFANSFVALMVAGAVIGLGNGLGSGSMMTLGADLAPAGATG
                     EFLGIWRLIGDLGMVVGPLLVGAFAGALSLDGSAIALMVAGLLASLTLAFLVKETRIM
                     PPPENEERNIVPSAS"
     gene            11361..12326
                     /gene="ftsY"
                     /locus_tag="VGR29_10835"
     CDS             11361..12326
                     /gene="ftsY"
                     /locus_tag="VGR29_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV2074123.1"
                     /translation="MVFRRLFRRQAEPDVQIQKGLEKTRKGVFDEITRLFDRTDIDEE
                     LYEDLEMLLIQADVGWDVSQQLVQELRDRVRDERIQNPADAREVLRLEMTRLLELATR
                     NRKVKILQRGVPWVILVVGVNGTGKTTSIAKLVQYHQGFGRTVMLAAGDTFRAAAIDQ
                     LKVWGDRLDVPVVAHQQNADPGAVVFDAMQAAHNRNADVLIIDTAGRLHTKFNLMAEL
                     HKLKKIIQKHVPEAPQEVLLVIDATTGQNGLVQAAEFTKSVDITDIFISKLDGTARGG
                     IAFSVAKELGTPISYIGTGEKAGDMAEFKPDTYVDSLFFGEGEGL"
     gene            12330..13703
                     /gene="ffh"
                     /locus_tag="VGR29_10840"
     CDS             12330..13703
                     /gene="ffh"
                     /locus_tag="VGR29_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV2074124.1"
                     /translation="MFETLSDRLNETFGKLGGRGKLTEGDIDTAMRDVRRALLEADVN
                     FKVTKDFVAAVKERALGQDVMQSLTPAQTAIGIVNEELIKVLGEEQVPLLQPDRPPQI
                     IMMVGLQGSGKTTHSGKLAVHLRKGGRSPLLVAADVQRPAAVNQLQALGKQVDIPVYQ
                     EGTSQTPVNIAANSVRFARERGHNPIIVDTAGRLQIDEPLMQELVDIKNTINPTEILL
                     VADAMTGQEAVGVAQAFHERLGVTGLILTKMDGDARGGAALSIRAVTGVPIKFIGTGE
                     KLDALEPFYPDRLAGRILGMGDVLSLIERAQEQTDDVDAERMQERMLKGQFDLEDFLD
                     QLQKIKKMGPLNQLLEMIPGVGQQLRQAKTQISDDDYKQIEAIIQGMTPWERRHPEQI
                     IHSRRRRIAAGSGVNRQDVNKLLTQFKEMQKMMGQFGKMAKGGKMPKNMRGMKGIKGM
                     PELPFDF"
     gene            13765..14478
                     /locus_tag="VGR29_10845"
     CDS             13765..14478
                     /locus_tag="VGR29_10845"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV2074125.1"
                     /translation="MSTDATQVNERFGGDPRITPAMEDYLKAIYRIEQEGEPATTHRL
                     AEEMDLSGASVSNMVKRLNDLNLLVHSPYRGVVLTPSGSAVALEVIRHHRLLELYLSQ
                     ALGFEIDQVHEEADRLEHHVSEELETRMEQALGFPEYDPHGHPIPSRNGAVPAIADML
                     LGDLPSGQTAVVSRISDRDPEHLRQIDHLGIGPGTPITAIGDEGDVRLVSVNGVKQAL
                     SGEITALIHVRPDGALATS"
     gene            complement(14468..15124)
                     /locus_tag="VGR29_10850"
     CDS             complement(14468..15124)
                     /locus_tag="VGR29_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEV2074126.1"
                     /translation="MVRAQNRNEIDPPSEGAEKISKWPRTNRRLERIESVLRRRQTSL
                     TVVLEDVHDPHNASAVLRSCDAVGVLDVHLVYVQEEFPRKSFGRSTSASAAKWIRIHE
                     HNSIEECYHHLRGQRFRIAATALEPESSDLYELDFTSPTALVFGNEMRGVSAGGIRGA
                     DCTVCIPMQGMVESLNISVSCAVALYEAMRQRQRAGMYDRAELGPDELARVRDEWLRK
                     "
     gene            complement(15237..15995)
                     /locus_tag="VGR29_10855"
     CDS             complement(15237..15995)
                     /locus_tag="VGR29_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074127.1"
                     /translation="MSLVWRGIRDVFDQLIFMAGLSLAWWICAAPAVFGFMLFLSAPV
                     LLPLALLTTFLMPPATVTLFAMADPRRIVNRPDREEMIGVFRSSVKRGWMIGLATVPL
                     LLILAWNIVYFQGTSSFLAAFVPLWAVLIIFVFILSIYMFSLAGTMESEVRNAFRGGM
                     FVLVSRPFTGALLAILIIVVAVVFTLMVLPMLLIGPALLAGVVNRFVLDVLEVEVIDP
                     NSPTVERAYEREKGINPETGIWERIKRGGRKEPA"
     gene            complement(16086..16685)
                     /locus_tag="VGR29_10860"
     CDS             complement(16086..16685)
                     /locus_tag="VGR29_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074128.1"
                     /translation="MDTNHNTRSRPRFEECDEIVRALVVEYVQRLGLPADCLWVTTDR
                     SLYSRWLRRRIPSSYGGAYCFLKGRGVHAVLINLERIDRSRPRAVEVVVAEELIHMRD
                     HLDRDYRRHAKHGYDRVAHRVSALTGASLEEIRSALIPVKRRPYKYVYACPHCEMRVA
                     RKRTGRWSCPRCSPRFHPRFVLQVVERMDKDMDPESVRT"
     gene            17129..17614
                     /locus_tag="VGR29_10865"
     CDS             17129..17614
                     /locus_tag="VGR29_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2074129.1"
                     /translation="MTDRPRLRARRHSERPHVLIISDEESLSSFLNEGLPMGGFWTTV
                     IASGLQALEVFRLRQFDLVIIDWELQSFGAMELLRRLRGVSTRTSSAEPRTTAPVVLI
                     SEQPADVPPEDIERLGIKRLLRAPLELDEVARELHAVFEEWRSAYPDVPLSDDPARSA
                     R"
     gene            complement(17566..18285)
                     /gene="phoU"
                     /locus_tag="VGR29_10870"
     CDS             complement(17566..18285)
                     /gene="phoU"
                     /locus_tag="VGR29_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV2074130.1"
                     /translation="MTTRLTYENQLQELRDLVVAMASMVNKAVARSMEAMRVQNVALA
                     REVRKGDKAINQMQRQGENMAITLIATQGPMARDLRMIASTMSILSNLERMGDYATGI
                     AKIVIETSNEELLKPLVDLPRMSEIAREMLDESITAYIEGDVDLARAVAIRDDAVDDL
                     YDQIFRELLTYMMEDARVITRATHLLWAAHNVERIADRVTNICERVAYTVTGEFEELD
                     GGRKKRQRALRAGSSESGTSG"
     gene            complement(18362..19225)
                     /gene="pstB"
                     /locus_tag="VGR29_10875"
     CDS             complement(18362..19225)
                     /gene="pstB"
                     /locus_tag="VGR29_10875"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEV2074131.1"
                     /translation="MAVDSGIHRDTVRISIQQGPSESPGPQQEVASPSAPVLRIRDLN
                     VFYGAFHAVKDVDMDIAQNAITAFIGPSGCGKSTVLRCINRMNDLIPTARVEGTVEYH
                     GQDVNGRGVDPVEVRRRIGMVFQRPNPFPKSIHENVAWGARINGYKGNISDLVQQCLE
                     RAALWGEVKDKLKDSGLSLSGGQQQRLCIARALATNPDVILLDEPASALDPISTLKIE
                     ELLRELATDYTIVIVTHNMQQAARVSDQTVFFSLSDERQGILVEAGPTQKMFTNPADP
                     RTEGYITGRFG"
     gene            complement(19229..20128)
                     /gene="pstA"
                     /locus_tag="VGR29_10880"
     CDS             complement(19229..20128)
                     /gene="pstA"
                     /locus_tag="VGR29_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV2074132.1"
                     /translation="MSTSVAAAQGSEQYRARLGVRRVLSEFWRWIFFASTALAIVLLG
                     MLLYSILVPGLPWLSWHLITEMPSRIPEQAGMNSAIWGSIWVVLGSAVLSFVLGLGTA
                     IYLEEYAEKNRWTAFIQTNISNLAGVPSIVYGLLGLVVFVDLMNMGRSVLAGAFTMAI
                     LILPIVVISAREAIRAVPSSLRQASYGLGATKWQTVRSHVLPVAFPGILTGVILAMSR
                     AIGETAPLIVVGGASQVLFRPGSPTDPFMVMPLQIYNWTGRPQPEFEHLAAAAIIVLM
                     IVLLIVNSLAIGLREYFSKKTRI"
     gene            complement(20125..21093)
                     /gene="pstC"
                     /locus_tag="VGR29_10885"
     CDS             complement(20125..21093)
                     /gene="pstC"
                     /locus_tag="VGR29_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV2074133.1"
                     /translation="MTTTPPRPEERPPLDLSGKKKRQASEQLVKYVLVACSLVTVATT
                     VGIIIILLYEALRFFRDIPFWNFLTGSTWTPLFSGSQQEFGVVPLVMGTLMTSVVAAL
                     ISLPLGLGAAIYLSEIASERGRSVLKPILEVLAGVPTIVYGFFALTFITPVLLQPLIP
                     GIGTFNGLSAGIAIGIMALPTVASLSEDALRAVPRSLREAGFALGATKFETSVRVVVP
                     AAVSGIVASFLLAISRAIGETMIVAVAAGQAPAGQSGGSDGWWDLTESMQTMTGFIAY
                     TFSSDISRGTTAFHSLFGVGLLLFFMTYLMNLLSQWVTKRYQEEYE"
     gene            complement(21194..22249)
                     /locus_tag="VGR29_10890"
     CDS             complement(21194..22249)
                     /locus_tag="VGR29_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV2074134.1"
                     /translation="MYLNDLRISRRTFSLAVAGTVAIAPMTRVFAQDDAVVYEPAEEA
                     ELSGEFEADGSSTLGPLTEAAIEEFAAVAPDVRVTNGISGSGSGFERFINGEIAIANA
                     SRQISDKEAALAQENGISWYRFPVAYDGITVAVSAENDFVTDLTVEELAKIWAADGGV
                     ATWADVRPDWPAEPIELYGPGTASGTFDYFSEAILGEEGEVRTDYIPSEDDNVLVQGV
                     AGSPYALGYFGFSYYENNQDTLKAVAIDAGSGPVAPSIETIADGSYAPLARTLFIYVN
                     ANELQGRPELQEFVKFYAATADEIAPSVGYIPLPDDVEQAVYEDVVAAIDGSLPPHSE
                     TRGAASPAASPEASPAA"
     gene            complement(22445..23191)
                     /locus_tag="VGR29_10895"
     CDS             complement(22445..23191)
                     /locus_tag="VGR29_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2074135.1"
                     /translation="MDAAHRSDSEGVTVFIVEDDPTLRSTLAFNLTREGYRVLTADSG
                     QAALDVVGDQGASLDLVILDVMLPGMSGMQVLRNIRAHLDVAVLLLSARGEEQDKVDG
                     FELGADDYVVKPFALRELLARVRAAVRRSALPSARPSGVLMRGPLSIWPDRRCAAVNG
                     CELRLRPKEFGLLVTLAMEPGRVFRRQELLDLVWGDDIIVEERTVDVHISWLRAKLEG
                     AGIGQECIRTVYGAGYRFVLGYEPTRTTAR"
     gene            complement(23412..24749)
                     /locus_tag="VGR29_10900"
     CDS             complement(23412..24749)
                     /locus_tag="VGR29_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV2074136.1"
                     /translation="MTSSVPDNLPPARYSSRPPGFSNLGPLHFYFRMAAVVLLILVSG
                     LAGGMVLERYVIRGSTPSDQSFPDLEAVAGVIDESYYYRPTSEEERERLDRQMEQQAI
                     IGALSSLEDEYTRYLGPELSATALEDLEGRYGGTGIDVAVDEGAVVVANVVADSPADD
                     AGIRRGDVIERIGNQPVDPGNFDAIVRQLRGEVGTSVALSLTRPETGESIELGLVREE
                     IVVPPVTLRMIEGTTIGWLRITIFGNDTLAEVEDAIRTLEQQGATGIVLDLRGNSGGW
                     VTSAQGVLGRFLDPDVGPALYEDTSPEAGEEIPLPILRDENSPSSNLPMVVLVDRGTA
                     SAAEIVAGALKDYGRAVVIGEQTYGKGSVQRIFSLDDGGTLRVTVAEWFTPDMSRIQE
                     QGIEPDVEVTAEEHASTGGDPALATAVGMLQSGDADIDAPEDPTSSPSATPES"
     gene            24812..25624
                     /locus_tag="VGR29_10905"
     CDS             24812..25624
                     /locus_tag="VGR29_10905"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEV2074137.1"
                     /translation="MPPPLLVEDMLMLLVVDVGNTNTVFGVYDLQSDALRTTVRAATR
                     RDRMPDEWFAILAPVLASVSIDPRSIEAMVISSVVPSVTRWLSTMSQERLKITPIVVS
                     VDIDLGLPIDMDDPREVGADRLVNSIAAIHRYGAPVIVIDFGTALNFDVVDARGHYIG
                     GSLSPGLVIALDAMTSRAARLFSVDLVPPPGAIGRNTTHAIQSGLVLGYVGLVEGMIQ
                     RISAEIDGKPTVVVTGGYAELFASQSPLIDAHDPDLTIDGLRLVYERLKARA"
     gene            25777..27855
                     /gene="fusA"
                     /locus_tag="VGR29_10910"
     CDS             25777..27855
                     /gene="fusA"
                     /locus_tag="VGR29_10910"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV2074138.1"
                     /translation="MKQYPPERIFNVGMFSHGGAGKTTLVEAMLFDTGATTRRGRVSQ
                     GTTASDTDPEEIKRHISLTNSVIPIEYKDCKINVIDTPDFADFGGDVRSAMRVVEAAL
                     IVIDAASGVEVGTEQVWHNAERAGLPRMIFVNKMDREYANFRNVLDGLIEAFGKRVAP
                     LQFPIGQEENFRGIVNLLDRQAMVFHDNRDGGYETTEIPDELADDVAFYRQTLVEAIA
                     ENNEELMMRYLDEEEISDDELRQELHQCFENGSVIPMVLGAAYENRGVETLLDAIINV
                     LPSAAERKETGMADGEAVEITPNAEKDLAALAFKTQADPHVGKVTWFRVFAGKISSSS
                     QIHNASTGQDERIGQLFYSRGKEHIPTEAVTAGDIGAVSKLDSVETGHTLTSRPSNLR
                     LHEIEFPNASYSASIHPRSQADLDKLGQALARMQEEDPSLRIERNPETGETIISGMGE
                     PHVQIAVERMKRRAGINVDLGLPRVPYRETITAKTTAEYKHKKQSGGAGQYGHVKLEL
                     EPLHDEDFAFTERVVGGAVPRNFYPAVEKGVREALESGPLGGYPVVNVQATLFDGSYH
                     DVDSNEMAFKIAAKEAFKQGILSGNPTLLEPIMAVSISVSENATGDVMSDLNTRRGQV
                     TGMDSGDNGTTVIEAEVPAGEIQRYATDLRSITQGRGTFTSRFVRYEPVPHHLAEKII
                     TEAKNNGSNG"
     gene            28075..28521
                     /locus_tag="VGR29_10915"
     CDS             28075..28521
                     /locus_tag="VGR29_10915"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEV2074139.1"
                     /translation="MSDSKFQQESLIETTPPKSLAFEIVFDGGSLGNPGKGYGSFEIM
                     SGGEIYKMGREEYTGSITNNQAEYMTLIESLKWLADDLSEDRGKATVTIHGDSQLVVN
                     QINGTWKVKNARMVPLVDEAKKLFKQFGRCTIAWHPRAKSVERLGH"
     gene            complement(28533..28745)
                     /locus_tag="VGR29_10920"
     CDS             complement(28533..28745)
                     /locus_tag="VGR29_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074140.1"
                     /translation="MKQRRSTDSIPTRSQAYEKALFSALVAALIALVVLKMLLGLVFV
                     ASAVVAVVAFVLFFSVAMRMTPRREE"
     gene            28779..29270
                     /locus_tag="VGR29_10925"
     CDS             28779..29270
                     /locus_tag="VGR29_10925"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEV2074141.1"
                     /translation="MARKSIYLASPLGFADSTRGFMNELVTSLEAIVDVENPWDDLRF
                     AGEFQAVQRIDSVKERRERLTRVNTEIGRCNVARIDASDGLFAILDGVDVDSGTAAEI
                     GYAFAAGKYICGLRTDFRLAGDNEAAIVNLQVQYFIVASGGRVATSSPELVELAKVFS
                     GSE"
     gene            29326..29399
                     /locus_tag="VGR29_10930"
     tRNA            29326..29399
                     /locus_tag="VGR29_10930"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29360..29362,aa:Val,seq:gac)
BASE COUNT         6089 a         9196 c         8591 g         5541 t
ORIGIN      
        1 gcctgctcct gggaatgatc gaggagggca ctggcatcgc catcacccat gacacgagcc
       61 tgacgccgga gcagatcacg gccctgcgcc aggacaacgg cggactgcag ttcaacatcg
      121 tgcaatggtg gctggcatcg agcaccccat cgctcctcgc cacagccgcg tctcgggaga
      181 gcgaatacaa tgcggggtgg atcaactggg aacctgacct ggaaccaagt ggcgagttca
      241 cgcccggcga ggatgcggca gctttcaacg atctggtaga ccgggcggaa gcggagctcg
      301 atcaggcaat caggaacgat ctctattacg aggccgaaac actgctgttg aggaacgccg
      361 tctacattcc tctcggatat tgggtgcagc gcttccttca gaagccctgg ctccagggaa
      421 cgcgccaggg accgtggacg gggagcaccc ctctgcggat cgacgaggaa gttgttgtcc
      481 gtggacgaca acaggggtaa acgaagcggt agcttggtca cctgacgaag ggcaaccgca
      541 tgtgcgattg cccttcgtca tttccgtcaa cttcttcacc gtggagaggt tttccctcgg
      601 cggcgccgtg cgcctagagc cggttgcaag ttggttgacc gtatggcatc gtgcgcgcat
      661 gccacgtgga tattcccgcg atctccgtga gcgactcgtg caggcggtgg cgtccggtct
      721 gtcggtggtt gaggtcgcac ggcgaatggg cgtcagctcg agtagcgtgc atcgctacaa
      781 gcgcaaggtc gcggcgggcg tatcgctggc gccaggcacc tcgcccgggg ggccgcgcaa
      841 gatcggtccc accgaggagg aggccttgcg ggctcaggtg ctggcaactc ctgatgccac
      901 gttggccgag cactgcgccg tgtggacggc cggcggccac tcacccgtca gtcgcgcgac
      961 catgagccgt gccttgcggc ggctggacct gccgctcaaa aaaagaccct aatcgcgacc
     1021 gagcgggatg aaaccgcgcg ggtgcagtgg cgcacggtga tggcggacct tgcccccgaa
     1081 acggtgctct ttctcgacga aaccagcaca cagacgacga tgacgcgatc atgcggacgg
     1141 gcaccgcggg gcgtgcgcgt gattggggcc gagccgcgta accacggtgc caatgtcacg
     1201 tgcctggtgg cgatgggccc gaccgggatg cagatgcccc gtgtcttcga gggggccgtc
     1261 accagtgacc tgttcctcgc gtgggtgcgc gagtggctgg tgccgacgct gccacgcggc
     1321 acgacggtgg tgttggacaa cctgagcgtg caccgccatg ccggggttca gccggcagtt
     1381 gaagccgcgg gctgccatct gcgctacctg cccgcctact cgcctgacct caatccaatc
     1441 gaactcgcct tcgccaaact caagacccat ctgcggggcg tcggcacccg gaccctcgac
     1501 ccactgatcg atgccatcgg cgtcggtctc gcccgcatca ccgccgccga cattgccggc
     1561 tattaccggc attgcggctt cactctgccc cagccggaga cgcaaccatc atgagaaccg
     1621 ctctaacgtg cgtgtacggc gtcttcagcc agacccgcgc tgccctgtct cggtgcaaaa
     1681 acgcggtaat cgatgttggc gaagggattg tcacggtgtt cgagagccgc cagcgtctca
     1741 cgctcatcag aggtcatctc ggtgccacgc tcgacgatat cggcgagcga gttgaatcgg
     1801 tcgacgtgct cattgaatcg ctggctggcg tattcattcg cctgcgccgt ggtgacgagg
     1861 aacggccaat cgctgctctc aagcagcagc aattcccggg ccgcttggtt caaaacatcg
     1921 agcagctcgc cgtttgcgtc cggatgctca gcaaccaggg cttccattcg cgactctgcc
     1981 ccgtgaatag ccggccacat ccactcggta tcagggttca tccacgtgaa gtggttgccg
     2041 cccatgcccc agctcgactc gggcaggatc atcacttcat cgccgccatg ctcgtcgacc
     2101 caggtggatg cggttgtcag atccaccgta tttaattccg ccaggccgcg gagaaccgcc
     2161 ttcagccagt caactccctc gaaccaccag tgcccgaaga gctccgtatc gtagttggaa
     2221 gcgatgatgc cgtatccgcc tgtttcttcc ttgtactgct gcagcaactc atcgacgaga
     2281 tgcacgaaat ggcgcgcatg gtccccgatt cgctcctgag ctttctccgg attgtatggt
     2341 ggcttgctgc cgaggtccac ctctgcgcca ccgacacgcc agtactgcat gccggagacg
     2401 ccgtccttgc ggtggaactc ccggtaccat tcatccccgg gatagccgaa ggacccggac
     2461 caaacctgct gaccagtgcg gttgttgcgg ccgagcaccg cggtttctcc cggcgcgtct
     2521 cccacccaat acgacttgta ggtcgtgcca ggctcctgct gttgctccgc tggctgattc
     2581 accagctggc ggcgcggtgc tgctccgtag atgcccactg catctccggc cgccttcccg
     2641 actggacggc ccccctcgac ggtatgcgtt tcgctgaaga agacgcgaat gtcctgcgcg
     2701 gcaaggaagg actccagacc gggccgggtg atctggacac cgttctcctc ttcaacgtag
     2761 gcgggacgat aggcacactc ggggagccag atcgcctttg gccggcgtcc gaagtgcccg
     2821 atatatgcgt cgacgccggt ctggatctgc ccatagatgg atgagtcccg ggagagaagt
     2881 ggcagatacc cgtgagtagc ggcgcacgtg gagatctcca cgaacccgcg atcctgcagg
     2941 tccttcaggg aaccaaggag atctccgttg aagcggtcac ggaacgccgt gagcgtccgc
     3001 gagtaccagt gatggaagtg ccgggcaagc gccatcagtt cctggtcacc agcctcctca
     3061 aatcgctcga tgtcagcggc agcccacgct gctcgctctt ccgcatacgt caggaaatgt
     3121 tcgacgatca agggatcagc gagttgctcg gcgaggattg gtgtcacgct cacagtgatc
     3181 cggtaatcca cgccgtcctc ggccaaatcg tggagggccg tccacagggg gacatacgtc
     3241 tctgcaatcg cttcgtgaat ccattcctcg ccatgtggcc acatgccggc ctgccgtgca
     3301 tagggcaggt ggctgtggag cacgaacgtg aatgctccat ttccgttact catcggcatc
     3361 tccttacatc gcatttcacg tgcgagttgc tgcggcacca gacgttagag gcggcagcat
     3421 acgattttgt caaagtgttc ggcgtcatat cagcttcaat gaaggcaatt cgttcgctcg
     3481 attgctatca ggataccctg ccggtcaacg cgcaagggcg cctaccagga tttggtggcg
     3541 ctgatccctg acttcggatc tgtatagtcc catggattct cggcggcatc cgggaccgcc
     3601 ctctccatac gcccataggt gttggtgagg tcccggaggc cgtctgccag ggacgggtcc
     3661 agttgcgacg ggagaaggcg ccaggcccag tttccggtgg ccgtcgcagg tgtattcatc
     3721 cggccctcgt ctcccatccg gaggatgtcc tgcatcgggg cgatcgccgt gtcagacacc
     3781 gaggcccacg ccagacggag caaatcccag gcaatgtcat ccgctgggcg cccgagatag
     3841 cggcgtacga catgtcgctc ttcttccggc agggagttcc accaacccac ggttgtctgg
     3901 ttgtcgtgtg ttgcggtgta cgtgacggaa tgacgcttga cgttatgagg caggtacatg
     3961 ttttccggat cgtcatcgaa tgcaaaatgc aggacattca tgccgggcag gtcgagaatc
     4021 tcccgaagcg ccacaacatc gggggtgatg ataccgagat cctcaacgat gacagggaac
     4081 tcgcccagct cccttcgggc tgccgcgaag acctcacctc ccggtgcgat ctcccagtgt
     4141 ccccccgttg cattctcatc cccaaacgga acgtgccagg cagcggcaaa tccccggaag
     4201 tgatcgatgc ggataatgtc caccatatcc cgcgtcgcgc gaagtcgatc aatccaccac
     4261 tggtagccat tcgccttcat cgcgggccag ttgtacgttg ggtttcccca gagctgaccg
     4321 gtggaggaga agaaatccgg tggtacaccg gcgacgaagg tgggacgtcc ttcttcgtcg
     4381 agctggaact ggtcctggct ggaccagaca tccgcgctat cgagtgcgac gaagatcgga
     4441 atatcgccga taatctggat tccccgcgtg ttggcgtacg cccgcacttc ccgccattgt
     4501 tgatggaaga ggaactgcac gaactcatgg aagcgcccgt cgtctgagag tttctcaccc
     4561 catgtcgcca cggcttcagg ctggcgggtg cgaatatcct ttggccagtc cacccaggaa
     4621 tcgccaccaa gtgcatcctt gacggacacg aagagcgcgt aatcgtgcag ccagctggga
     4681 gcgtcggctc ggtacgcctc aaacgcgtcg cgcaggttag acgacgcacc gttctccaag
     4741 cgatgatacg caagccggag caatcgcatg cgaaacggag ccacctgctc gaaatcgaca
     4801 gcatgcctgg ggaatgcgta ggcaggggca agctcctctt cggtcaatag accgtccccc
     4861 tggagccatg ccagagacac cataagcgga ttgccggcga acgccgatgg cgaggaatac
     4921 ggcgaattgc cgaagccggt ggggcccaac ggcatgactt gccacagacg ctgaccagct
     4981 ctctcgagcc actccacaaa gcgaaacgct gcctccccaa agtcgccgat gccgtgcggt
     5041 ccgggcaacg atgttggatg gagcagaatc ccgctctctc gggggaaatg caagggatta
     5101 tccttcctga ttgatcctgg ttatcaaatt tgcaagggag attgccaccg ggccagacgg
     5161 acccagcggc tcctggcact gccaggcttg atcacgtccc caacgtcccg aaatcactac
     5221 tcggtagcat cgaggaactc gagtgccacc cagccggtct cctcgccgac acgtacctgt
     5281 acccactcga tcccgtcctc tccttggcga gtgaccggcc cgccgatcac ctccaccaca
     5341 gtggaaacgt cgaggggagc ttgcccgatc aaatcacaat cggtgcctgg gccagaacgg
     5401 aaattcagct cgctgttcac gcggtaggtg gatgtcgcgc cggtgaatgc gggcaggtct
     5461 tcaacttcac agccgtcgac tacgggaatc tccccatccg ccaccgcggt gccgggtcca
     5521 tcctcgggcg tggaacctcc ggcagccgcg ggcgccggcg ctgcctcccc agccgcaggg
     5581 gttccggcac tggaaccggc ttgcgcagga ggaggtgcac cggttgtgcc atcgcctgtc
     5641 ggaggttgat cagccgctgg agtgccatcc acctcggggg tcgaagcagc caggccagga
     5701 acaggcactg ccgaatccgc tggcgtggca tctccggcac ctggcgaagg gcgcacctta
     5761 tcgtccgtct ccagagggtc ccctgccgca ccgggcgagg atggatcgat attggtcggt
     5821 gcctccgggg acccgttgtt ctcgtccgaa ccgtcggtgc ccggtccctg cacggtaggc
     5881 tgagcggaag acggctcatc tgtctcattc gggccctcgt ccgtatcgcc gataccacac
     5941 gctgtcacga gcaggcagag cactgccgtc aaggcgatca tcatccctcg atagcggtgt
     6001 ccttttttca ttcgtttgtt catatctccc tcctcgtttc gcgccttccc ccctcagcca
     6061 acacgagatc acgctgcccg cgcaagccgc caagccttct atggctcctc aagactatct
     6121 ctcaggatgt ccatatgctc gagggcgagc ccggtgccaa tggccacgca tgagagagga
     6181 tcgtctgcaa catagcatgg cacaccggta acctcggtca gcagatcggg aaggtggcgc
     6241 agcatggcgc cgccgccggt caggatcatg cccttgtcga tgatgtccga agacagctcc
     6301 gggggcgtct cctcgagcac ggcgcgaacc gcgccgatga tcgcctctag cggtccagtg
     6361 atcgcatcgg tcacctcgct ggcgtgaatc tggatcgttc gcggaagtcc cgcgacttcg
     6421 tcacgccctc gaacttccat gaccttgtca tcatccagcg gcatggcact gccaatggcg
     6481 atcttgactt cctcggcggt gcgctcgccg acacggaggt tgtactttcg tttgatatgg
     6541 ttgctgatcg cgtcatcgaa cttgctcccg ccgaccctca gggaccgcgc aaccacgatg
     6601 ccgttaagcg agatcaccgc aacttcggtc gttccaccgc cgatatcgat gatcatattc
     6661 ccggaaggat ccgccaccgg aacccgtgcg ccgattgctg ccgcgagcgg ctcactgatg
     6721 agataggccc ggcgcgcccc ggcgttcaag gccgactctc gaacggcacg tcgctccacg
     6781 ccggtaacgc cggcaggaac acagatcatc acatcaggtc gaatgaggga aaaacgtccg
     6841 accgccttgt tgataaagta ctcaagcatc ttctgggtga cgacatagtc ggcgatcacg
     6901 ccatcccgca tggggcgcac gacttcgatc gtctctcggg gctcgcggcc cagcatgtcg
     6961 cgagcctcaa tcccaaccgc ctttaccttc ccgtccttcg ccgagatcgc gaccacggaa
     7021 ggttcgttga ttaccacgcc acgcccgcgc acgaatacca gtacgttcgc ggttcccagg
     7081 tcgattccta cttgtttcgg catgcactgg tcctctgatt ccggatcagg gttaaaggct
     7141 tcctggtatg tcaggaggga cgacccggct ttgagaacgt ctgacctgac gccgggcaca
     7201 cagatcaccg gaatgcgcag ccctaatcat cggagcagtg cgcataccgc tgtgatcaag
     7261 ggtataccag tgcttgcgtg cggtgcacgg gatgcacaaa cacacggcga ccggcgcagc
     7321 agcattacgc cctcaatctc aaggccagaa tgctttacac cacggtatca acgagggcga
     7381 taaactcttc cagtgcttcg ttcgccgtga ttgcccggac caccggctcg tcgaccgtgc
     7441 ccaggaatcc ctcaagcccc tcccggattt cctccctcgt ctccccgtag accgcggaat
     7501 ctaacgcctg gaaatcgagc cttgcgaaat tggctgcgta ggagggaatg gcttcgtagc
     7561 gcgccacctc gcgctccagg gccggtcggg catcctgtcc gagcgccgtc cgtatgtaga
     7621 gtgcgacctc agcccgcttg tcgggaaggt cggcaagatc actgtccttg tcggcaagag
     7681 cacgacgcgc cgcctccggt gtgagccagt tcagtagcac tccctgggtc tcccggaccc
     7741 cgagtcgccg cattcgaggc ccgagggcac cgaccatcac aggtgctccc aaacgctcat
     7801 ggatgaacga cgcggcgttc gagacggtgt tgagaggact tggaggcgca cttgacccga
     7861 tgccgaggac caggcggtcc agcggcagtg cccgctcctg cacggtacgc acgatctctt
     7921 gaggcaccat cctgtccacg gggatcactc cggatgcaag ccggatcctg gtggtcacgg
     7981 atgccgcaac ctggatggaa gcgagcgaat cgccagaacc tccatgattg atccaaagtg
     8041 tgcgaaagcc agcctcctcc agacgcgggg ccaactcgcg gacgacacca tgatcgaccg
     8101 tgcttgtgat gccgaaaccc attttcgtga gatcgaggtt cacgggaaca ccccttcact
     8161 ttcgtctact tcgggaaacc aaggccaaga cgcttcaacg agacatgccg tccctgtcca
     8221 atgatgagat gatccaccag cttgatatcg agcagatcac ccgccttcac caaatcgaga
     8281 gtgacattca catccgctgc cgaaggcgta ggatcaccgg aggggtggtt atgtaccaaa
     8341 actattccta cagagttatg gcggaccgcg gcccgaacgc tggccgcgtt cacgcttccc
     8401 tggtacacca ttctggatgt ctgtatgtgg ttcttggtat cgagcaacac cacccggagc
     8461 tgctcctgct caagcgccgc catctcgatc cccaggagct ggacaacatc ttcgggagac
     8521 tcgatgcgag gctttccctg gtcagagata gcggccagcc ggcgtccaag ctcgaacgcc
     8581 gccttcactt tggaagcctt tgactccccg acgccccgca cccctgccag ctcgacgaac
     8641 tccatccgca tcagcccggt cagaccgcca caatcatgca ggaggcgctg ggccatcgtg
     8701 gtcaccgact cgcccttgat acccgtattg agaaggatcg ccagcagctc gctgttgctc
     8761 aacgcggctg gcccgccgct tctgagtcgc tcccgcgggc gctcatcggg ctgcatctcc
     8821 cggatcatca gccgatatgg tgcctgctct acttcatgca tgtggaaccg ccgtcgggga
     8881 atacgccgcc acaatcatgc cgaaatagga cggcacttca taggacagaa actctgcatc
     8941 gcagggtgtg acgtcctgga ttcctgcgag catcagtgtc tgccacagtc catcgatagc
     9001 agcgtcggtg acatcctcgt cgctcagcgt tgcaagcgta tggagctcat gctccttcac
     9061 cgcctgcacc acctttgcat cgacttcagc cgccttcggg tgagcaccgt atgggccgtc
     9121 ctcccggtgt gtgtgagccc agtcacagga tgcgatgaaa ctgattttcc tcctgtcctc
     9181 ggcaaaaacc cggcccaaca tctgcccgaa atcaaccatt ttttgccgcg acagcgaccg
     9241 tgagggtgtg atgagcacca ccggtggccc ctcatccttc tccgggttgg gtgccaggac
     9301 attgccggaa aggggccggt tggcatcgtg acccacaaac caaagaggcg ttactgctcc
     9361 ccaatccagc ggagccacac tctggtcggc ccgattgccg ccgaatccgg ccgccgcagc
     9421 gttgatgccg gcggaccgcg cgccagcgag gatcgcctcg accagctgtc ggtcgcaagg
     9481 gacgttcatc tcgatgctac ggtcgccacg cgccagcgtc ccggcagccc gcccgacatc
     9541 cataacgcac atcgcgcctt caacccgtgt gccgtgggga cctgcgagta cgaccgcctc
     9601 gaccccggcg tcggcgaacc gccggcccac ctcctccatc gctctccggg tctccatggc
     9661 accctcggcg tcatcactca gcgcagggat cagcgcgaac ccgtggggca tgattgccgc
     9721 cgcgaccaaa ggcataacga cctcctgtaa cgaacaggtg tgtaggcgtt accagtgact
     9781 ctcacgtgcc atcgaagtcg ctaacttctc actcgacgat tatcctcacc agcgataaat
     9841 ccttctcaac tggctgacgg cacaatattc ctctcctcat tctcgggcgg cggcattatg
     9901 cgtgtttctt tcaccaggaa tgccagtgta agggaagcaa gcaaacccgc caccatcagg
     9961 gctatcgcgc taccatcgag gctcagggct ccggcgaagg ctccaacaag gagcggtcca
    10021 acaaccatcc cgagatcgcc tatgagccgc cagatgccca gaaactctcc cgttgcccct
    10081 gccggagcca gatccgctcc cagcgtcatc atcgatccgg agccgagccc gttccccagg
    10141 ccgatcacgg ccccagccac catcaacgca acgaagctgt tcgcaaaagc gaccgacccg
    10201 attccaagag ccataatggc aaagctcggt acagaggcga acttccggcc gaaccgatcc
    10261 atgagaacgc cggcggggaa gaacatcgac atatcgatca ctgccgatac agtcatcacc
    10321 gtccccacct cagctgcgtt caaccccagc tgctgggcgc cgatgagagg gatgaggatc
    10381 tgtcggccct ggcggatcat ctgggcgaac agctgcgcga ccccggccgc gctcaggtcc
    10441 gccgcgttct ccttcaatgt ccgccgcacg atcctccacc gctcgccgct cgcccccttc
    10501 ttcggtatct ggtcgggagc gtcgcgggga atgaacatcg atgcggccac ccaggcacaa
    10561 ccgccaagca cccccgcgac caggaaggcg acctgcatgc cgaacgcggt ggcgataact
    10621 cctccaatgg ccggtccacc gaacattcct acccggttga taccgccgaa gagcgcgatc
    10681 gcgctcccgc gcctctcggg gggaatcgcc tgggcaatga atgagtgacg gctgagaccc
    10741 cagagcgccg tgccgatacc ggcgaagacc cgaagcgcca cgatcagttc aggcgtgaga
    10801 tcaagggtca gccccagcgt gccgataaca accagtgcac agccgatcag catcgaacgc
    10861 cgcagcccga tccggtgcag gactattcca gctggcacgt ccgcgaccag cgtgccgatc
    10921 gccgcagccg atgaaatgag actgatcagc atatagctcg cgccgaggtc gatagcgtac
    10981 agcggcaggg tggccagcag gagtccttgc gcgagcgcaa ggagcgccgt tggcagatag
    11041 accgcaagca caagctcgga ttgacgcatg aatggacctc gcgatgcacg acgccagcgg
    11101 ggttgatcct ccaacccggt cgtatctggg tttgggcgat cgtaccaaga gatgcgcatc
    11161 tgcgaaagcc cgaatccgga acgcgaacac ccccggtgta tactttgcag gcaagggatg
    11221 ggccggatgc atggttattt tgtgttgctc tccggccaat tctccgcgct cacagggatc
    11281 acgctctccc atgcagaccc ctccatcgac acccagtgca tgaagcagcg tcaccgcgct
    11341 aattttcagg agttcacacc ttggtcttca gacgactctt tcgacggcaa gcagagccag
    11401 atgtccagat tcaaaaaggt cttgagaaga cacgcaaggg cgtgtttgac gagatcaccc
    11461 ggctcttcga ccgcaccgat atcgacgagg agctgtacga ggaccttgag atgctcctca
    11521 tccaggctga tgtcggatgg gatgtgagtc agcagcttgt ccaggagctc cgggatcggg
    11581 tgcgggacga gcgtatccag aaccctgccg atgcccgtga ggtcctgcgt ctcgagatga
    11641 caaggctgct ggagctcgcg acccgaaacc ggaaggtcaa gattctccag cgtggtgtgc
    11701 catgggtcat tcttgtcgtg ggcgtcaacg gtactggcaa gacgacctcg atcgcaaaac
    11761 tggttcaata tcaccagggt tttggccgta ccgtcatgct tgcggcggga gacacgttcc
    11821 gggcggcggc gattgaccag ctcaaggtct ggggcgatcg cttggatgtc ccggtggtgg
    11881 cccaccaaca gaacgcagat ccgggggcgg tggtcttcga cgccatgcag gcggcccaca
    11941 accggaacgc tgacgtcctg atcatcgata cggcaggccg gctccacaca aaattcaatt
    12001 tgatggccga gctgcacaag ctcaagaaaa tcatccagaa gcacgtgccg gaggcacctc
    12061 aggaggtgtt gctcgtgatc gacgcgacaa ccggacagaa cgggctcgtt caggcggcgg
    12121 agttcacaaa gtccgtcgat atcaccgaca tcttcatctc caagctggac ggcaccgccc
    12181 ggggaggcat tgccttctcc gtcgcgaagg aactcggcac gccgatctcc tacatcggaa
    12241 ccggcgagaa ggccggcgac atggctgagt tcaagccgga cacatacgtc gattcactct
    12301 tctttggcga aggcgagggc ctgtaacaca tgtttgaaac actctctgat cgtttgaacg
    12361 agaccttcgg caagctcggc ggtcgcggca agctcaccga aggcgacatc gatactgcga
    12421 tgcgagatgt ccgtcgggcg ctgctcgaag cggacgtcaa cttcaaggtc accaaggatt
    12481 tcgttgccgc agtcaaggag cgggcgctcg gccaggacgt catgcaatca ttgactcccg
    12541 cgcagaccgc catcgggatt gtcaatgagg agctgatcaa ggtcctcggc gaggagcagg
    12601 tgcccctcct ccagccggac cgtccgccgc agatcatcat gatggtcggc ctgcagggtt
    12661 ccggtaagac cacccattcc ggcaagctgg ctgtccacct tcgcaagggt ggccggagtc
    12721 ctcttctggt cgcggccgat gtccagcgac ccgccgcggt gaatcagttg caggcgctcg
    12781 gaaagcaggt ggacattccc gtttaccagg aaggcacttc gcagactccc gtcaacattg
    12841 ccgcgaattc agtccgcttc gcccgcgagc gcggtcataa cccgatcatt gttgatactg
    12901 ctggccgcct ccagatcgac gagcccctga tgcaggagct cgttgatatc aagaacacca
    12961 tcaatccaac ggaaatcctc ctggtcgcgg acgccatgac tggccaggag gccgtcgggg
    13021 tagcgcaggc cttccacgag cgcctcggcg taaccggact catcctgacc aagatggacg
    13081 gtgacgcccg cggcggtgcc gcgctctcca tccgcgctgt tacgggcgtg ccgatcaaat
    13141 tcatcggcac cggcgagaaa ttggacgcgc ttgagccgtt ctaccctgac cggctcgccg
    13201 gccggattct cggcatgggc gatgttctct cgctcatcga gagagcccag gagcaaaccg
    13261 acgacgtcga cgcggagcgc atgcaggagc gcatgctcaa gggtcagttc gaccttgagg
    13321 acttcttgga ccagctccaa aagatcaaga agatgggacc gctgaaccag ttgctggaga
    13381 tgatccccgg tgtgggccag caactgcgac aggcgaaaac ccagatatcc gacgacgact
    13441 acaagcagat cgaggcgatt atccaaggga tgacaccctg ggagcgccgc cacccggagc
    13501 agatcatcca ctcccgtcgg cgacggattg cagcaggcag tggcgtgaac cggcaggacg
    13561 tcaacaagct tctgacccag ttcaaggaaa tgcagaagat gatgggccag ttcggcaaga
    13621 tggccaaggg cggcaagatg ccgaaaaaca tgcgcggcat gaaggggata aagggtatgc
    13681 cagagctccc gttcgacttc taggcatcct ggctggcaca caggcgctcg ccatgtctct
    13741 ccgctccggc acgagggtct ctgcatgagc actgacgcaa cacaagtgaa tgaacgcttt
    13801 ggcggcgatc cacggataac ccctgccatg gaggactacc tgaaggcgat ctaccggatc
    13861 gagcaggaag gtgagcccgc taccacccac cgcctcgccg aggagatgga cctgagcggt
    13921 gcctccgtta gcaacatggt caagcgactg aacgatctca atctgctcgt ccacagtccc
    13981 tatcgcggtg tcgtcctgac cccgagtggc agtgccgtag ctctggaagt catccggcac
    14041 catcggctgc ttgagctgta tctctcacaa gccctgggct tcgagattga ccaggtccat
    14101 gaggaggccg accggctgga acaccatgtg tccgaagagc tggagacacg gatggagcag
    14161 gcgctcggat ttccggaata tgacccgcac gggcacccga tccccagccg caatggagca
    14221 gttcccgcaa tcgccgatat gctgcttggt gacctaccgt cgggtcagac cgcggtggtc
    14281 agccgcatca gcgaccgcga tccggaacac ttgcggcaga tcgatcacct cggcatcggt
    14341 cccggtactc ctatcactgc catcggcgac gaaggcgatg tacggctcgt ttcagtgaac
    14401 ggcgtcaagc aagcgctttc gggagagatc accgccctga ttcacgtaag acccgatggg
    14461 gcactcgcta cttcctgagc cactcgtctc ggacgcgagc cagctcatca ggaccaagtt
    14521 ccgcccggtc atacatcccc gcccgctgcc gctgtcgcat tgcctcgtac agcgccaccg
    14581 cgcacgaaac cgagatgttg agactctcca ccatcccctg catggggatg cacaccgtgc
    14641 agtccgcgcc tcgaatacca ccagcactca cgccgcgcat ttcgttaccg aagacaagtg
    14701 ccgtcggcga agtgaaatca agctcgtaga gatcactgct ctcgggctcg agtgccgtcg
    14761 cggcaatcct gaaacgctgc ccgcgaagat ggtggtagca ttcctcgatt gagttgtgct
    14821 catggattcg tatccacttt gcggcagatg cggatgtgct gcgaccaaac gattttcggg
    14881 gaaactcctc ctgcacgtag acaagatgca catccagcac acctactgca tcgcatgaac
    14941 gcagaactgc gcttgcgttg tggggatcgt ggacatcctc cagaacgacg gttagcgacg
    15001 tttgccgcct ccggagaacg ctctcgatac gctccagtcg ccggttcgtt cgtggccact
    15061 tgctgatctt ctccgcgccc tccgaaggag gatcgatttc attccggttc tgcgccctca
    15121 ccattttggg gatcgctcat cggcatgggc gcgcgccgcc ttgggcggct ctgcttcagc
    15181 ccttccgctc gaagtgcagt tcctgaatat tcgcggccgg tctatcgcga cgctcatcat
    15241 gccggctcct tccggccacc tcgcttgatc cgctcccata ttccagtctc cggattaatt
    15301 cccttctccc gctcatacgc ccgctcaacg gttggagagt tcgggtcgat aacctctacc
    15361 tccagcacat ccagcacgaa gcggttcacc actcccgcga ggagtgccgg accgatcagc
    15421 agcatcggca acaccatcag ggtgaacacc accgccacaa cgatgatgag gatagccagg
    15481 agcgcgccgg tgaacggccg ggacactaat acgaacatgc cgccccggaa agcattgcgg
    15541 acctccgatt ccattgtgcc ggccagcgag aacatgtaga tcgacaggat gaagacgaag
    15601 atgatcaaga ccgcccacag cggcacaaac gccgcgagga acgaggatgt gccctggaaa
    15661 tagacgatgt tccaggcaag gatgagcagc aacgggacag ttgccagacc aatcatccag
    15721 cccctcttca ccgaggatcg gaagacgccg atcatctcct cgcggtcggg tctgttcacg
    15781 attcgccttg gatcggccat agcgaaaagc gtaacggtgg cgggtggcat cagaaacgtc
    15841 gttaacaacg ccagcggcag gagaactggc gcagagagga agagcatgaa gccaaacacg
    15901 gccggtgcgg cgcagatcca ccatgcgagt gaaagaccgg ccatgaagat gagctgatcg
    15961 aatacgtccc gaattccgcg ccaaactaac gacaaaaacc gcaccattta ccgcctttgt
    16021 aatcaggatt taggccacga cccggatcct ggggacctca actccgacgg ctcaattgta
    16081 cgacttcagg ttcgaacgct ttccggatcc atatccttgt ccattcgctc gacgacctgc
    16141 agcacaaaac ggggatgaaa acgcggcgag catcgcgggc aagaccacct ccccgtccgc
    16201 ttgcgtgcca cgcgcatctc gcaatgtgga caagcgtaaa cgtacttgta gggccgccgt
    16261 ttgacgggaa tgagtgcgga ccggatctcc tccagggatg caccggtaag cgccgatacc
    16321 cgatgggcca cgcgatcata cccatgcttt gcgtggcggc gataatcacg atcaaggtga
    16381 tcgcgcatgt ggatcaactc ctcagcgacg acgacttcga cagcccgagg tcgcgacctg
    16441 tcgatgcgct cgaggttgat caacacggca tggacgccac ggccctttag gaagcagtac
    16501 gcgccaccat aggaggatgg aatgcgccgg cgcagccatc ggctgtacag cgaacggtcg
    16561 gtggttaccc agagacaatc tgcgggaagc cccagtcgct ggacgtactc caccaccaac
    16621 gccctcacaa tctcatcaca ctcttcaaat cttgggcgcg aacgcgtgtt gtggtttgtg
    16681 tccatccgac ttccttgaac ctgtctctca accagaataa cgggcctctg cttctgacca
    16741 cagctttcac catcgtactg accgtgcgtg ggcaaagata tgccatgacg cggccagtaa
    16801 agaagcgaga cgagcggttt aggttcgctc agcagttggg aacactgttt ggagccctcg
    16861 ttcagcgaga acccgcgaag gagcgccgaa gcttatcaca gacgggcgca gcggtctggc
    16921 gatcagggag aaaacagccg ccaaatttgt aacggacgcc cgacagcact aagggcattg
    16981 tcatcggtgt aacggtgggc gagatgatcc tcgccgggct acgactgcct tatcatgtgc
    17041 gaacggctgc cagcgacagg aagcctgccg gtaggcacgt ccggggttat cttaatccct
    17101 aattgttcaa cgaaaaggca cccacatcgt gaccgaccga ccacgtttgc gagcgcggcg
    17161 ccatagcgaa cgaccgcatg tcctgattat ttccgacgag gaatcactct cctcgtttct
    17221 caacgagggc cttcccatgg gaggcttctg gactacggtg atcgccagtg gccttcaggc
    17281 actggaggtc tttcgccttc gccagtttga cctggtcatc atcgattggg agctccaaag
    17341 cttcggagcc atggagttgc tgcggcggct gcgaggcgtc tcgacgcgaa catcttcagc
    17401 ggagccccgc acgactgccc ccgtagtcct catatccgaa cagccagcgg acgtgccgcc
    17461 cgaggacatc gagaggctgg ggatcaaacg cctgcttcgt gcgccgctgg agctggacga
    17521 ggttgcgcgg gagttgcacg ccgttttcga ggaatggcga tcggcctatc ccgacgtgcc
    17581 gctctccgac gatccggcgc gcagcgcccg ctgacgtttc tttcggccac catcgagctc
    17641 ttcgaactcg ccggtgacgg tataggcaac gcgctcgcat atgttggtca cccggtcggc
    17701 tatccgctcc acattgtgcg ccgcccacag aaggtgggtg gcacgcgtaa tgacccgagc
    17761 gtcctccatc atgtaggtca atagctcgcg gaaaatctgg tcatagagat cgtcgacggc
    17821 gtcatcccgg atcgcgaccg cgcgagcaag gtctacatcc ccttcgatgt acgctgtgat
    17881 ggactcgtcg agcatctccc gcgcgatctc gctcatccgc ggtagatcaa caagcggctt
    17941 cagcagttct tcgttcgacg tctcaatgac gattttggcg ataccagttg catagtcgcc
    18001 catccgttcg aggttggaaa ggatcgacat ggtggaagcg atcatacgga gatcgcgcgc
    18061 catcggtccc tgcgtggcga tgagcgtgat ggccatattc tcgccctgtc gctgcatctg
    18121 gttgatcgcc ttgtcgccct tgcgaacttc cctcgcaagg gcgacgtttt ggacgcgcat
    18181 cgcttccatc gagcgggcga cagccttgtt taccatgctc gccattgcga cgacgagatc
    18241 gcggagctcc tgcagctgat tctcgtaggt gaggcgcgtt gtcatcgatt tctccggaac
    18301 aaagtgtcct gttgctcgtt ctcgtcattt ctgttgtgag agtaagcgac gctcatcgga
    18361 actacccgaa tcggccggtg atatagcctt cggttcgagg atccgccggg ttggtgaaca
    18421 tcttctgtgt cggccctgct tccacgagga tgccttggcg ctcatcgctg agcgaaaaga
    18481 agacggtctg gtcggacacc cgcgcggcct gctgcatgtt atgggtcacg atcacgatcg
    18541 tataatccgt cgccaactct cgcagcaact cctcgatctt gagagtggag attggatcga
    18601 gcgccgatgc cggttcatcg agaagaatga cgtccgggtt cgtcgccaat gcccgcgcga
    18661 tacagagtcg ctgctgctga ccgcccgaaa gcgaaagccc gctgtcctta agcttgtcct
    18721 tcacctcgcc ccacagcgcg gcccgctcga gacattgctg caccaggtca cttatgttgc
    18781 ccttgtaccc attgatccgt gcgccccagg cgacattctc gtggatgctc ttcgggaagg
    18841 ggttcggccg ttgaaacacc attccgatgc gccgccgaac ctcaaccggg tctactcccc
    18901 tgccattcac gtcctgaccg tgatactcga ccgtcccctc gacacgagcc gtcggaatga
    18961 gatcgttcat acggttgatg catcgcagga cggtgctctt gccacagcct gacggaccga
    19021 tgaacgccgt gatcgcattc tgggcaatgt ccatatcgac atccttcacc gcgtggaagg
    19081 cgccatagaa gacattgaga tccctgatgc ggagcacggg agcgctcggg ctcgctacct
    19141 cctgctgagg tccgggcgac tcactgggcc cctgctgaat cgagatgcga acggtatctc
    19201 ggtggatgcc ggagtcaaca gccatgggtt agatcctcgt tttcttgctg aagtattcgc
    19261 gcagaccgat cgccagggag ttgacgatga gcaggacgat catgagaacg atgatcgccg
    19321 ctgctgcaag atgttcaaat tcaggctggg gtctacccgt ccagttgtag atctggaggg
    19381 gcatgaccat gaacgggtca gtcggactgc caggccggaa gagcacctgg ctcgcccctc
    19441 ccacaacgat cagtggggct gtctcgccaa tagcccggga catcgccagg atgacgcccg
    19501 tcaggatgcc gggaaatgcg acgggcagga cgtggctccg caccgtctgc cacttcgtgg
    19561 caccaagccc atacgacgcc tgcctcaggc tcgacggcac cgcccggatc gcctctctgg
    19621 ctgagatgac gacgattgga agaatcagga tcgccatggt gaaggccccg gcgaggactg
    19681 acctccccat gttcatcagg tcgacgaaga ccaccaggcc aaggagccca tagacgatcg
    19741 aagggacgcc cgccaggttc gagatattcg tctggatgaa agcggtccag cggttctttt
    19801 ccgcgtattc ctcaagatag atcgccgtac caagtccaag cacaaaggac aatacggcgg
    19861 atcccaatac gacccagatt gatccccaga tcgctgagtt catacccgcc tgctcaggga
    19921 tccgcgaagg catctcggtg atcaggtgcc agcttagcca gggaagaccg gggacgagga
    19981 tcgaatagag cagcatacca aggaggacga tcgcgagagc ggtcgatgca aaaaagatcc
    20041 agcgccaaaa ttcgctgagt acgcgccgca ctccaaggcg ggcgcgatac tgttcgctgc
    20101 cttgggcagc ggcaacactg gtgctcattc gtattcctcc tggtaccgct tcgtcaccca
    20161 ctggctgagc aggttcatca ggtacgtcat aaagaagagc agcagtccga ccccgaagag
    20221 tgagtgaaac gcagtcgtcc cacgggaaat gtcgctgctg aaggtgtatg caataaaccc
    20281 ggtcatggtc tgcatgctct cagtgaggtc ccaccaaccg tcacttccac cactctgccc
    20341 cgccggcgcc tgcccggcag ccacagcgac aatcatcgtt tcgccaatag cccgggagat
    20401 cgcgagaagg aatgacgcaa cgatcccgga caccgccgcg ggcacaacga cacgtacgga
    20461 ggtctcaaac ttcgtcgccc cgagggcaaa ccctgcctcg cggagtgacc tcggtaccgc
    20521 gcgaagcgca tcctcggaga gggaggcaac ggtgggaagt gccatgatcc cgatggcgat
    20581 accagcggac aaaccgttga aggttccgat tcccgggatc agcggctgca acaagacggg
    20641 cgtgatgaac gtcaaggcga aaaacccgta cacgatcgtc ggcacgccag caagcacttc
    20701 cagaatcggt ttgagaaccg accggcctcg ttcgcttgcg atctcgctca agtaaatggc
    20761 tgcccccagc cccaacggca gggaaatgag cgcagccact acggacgtca tgagcgttcc
    20821 cataaccagt ggcaccacgc caaattcctg ctgggagccc gaaaagagcg gcgtccacgt
    20881 tgatcctgta aggaaattcc aaaatggaat gtccctgaag aatcgaagcg cttcatacaa
    20941 caggatgatg atgatcccga cggtcgttgc cactgtgacc agggagcagg cgaccaggac
    21001 gtacttgacc aactgctctg acgcctggcg ctttttcttg ccagaaagat ccagcggcgg
    21061 cctctcctcg ggtcttggcg gcgtggtcgt catcgatacc actcagagtc tccagactgg
    21121 atgtagtgag aatttctcgt ggtgcatccg gtcggcgccc cgactggatg caccacgagg
    21181 gatcaccagg tggctaggca gccgggcttg cctctggaga agctgccggg ctcgccgctc
    21241 ccctggtctc gctatgcgga ggcagggatc cgtcgatagc cgcaaccaca tcctcataga
    21301 cggcctgctc gacgtcgtcg ggaagcggga tgtacccgac ggacggagca atctcgtccg
    21361 ccgttgcagc atagaacttc acgaactcct gaagctcggg ccgaccctgg agttcgttgg
    21421 cgttcacgta gatgaagagt gtgcgggcaa gcggtgcgta ggagccatcg gcgatagttt
    21481 caatgctggg cgcgacagga ccactaccgg catcaatcgc gacggccttc agcgtgtcct
    21541 ggttattctc ataataggaa aagccgaagt agccgagggc atacgggctg ccggccacgc
    21601 cctgaacgag gacattgtca tcctcgctcg gtatgtagtc tgtgcggacc tcaccctctt
    21661 caccaagaat cgcctcgctg aagtagtcga aggtcccgga cgccgtgcca ggtccataca
    21721 gctcgatcgg ctctgccggc caatccggac ggacatctgc ccaggtggca acgccgccat
    21781 ccgcggccca gatcttggcg agttcctcta cggtgagatc ggtaacaaag tcgttttcgg
    21841 cggagacagc aacggtgatt ccgtcgtagg ccaccgggaa gcggtaccag gaaatcccgt
    21901 tttcctgcgc caacgcggcc tccttgtcgc tgatttgacg ggaagcgttg gcaattgcaa
    21961 tctcgccgtt gatgaagcgt tcaaaaccgc taccggagcc agagatgcca ttggtgactc
    22021 gaacatccgg ggccacggcg gcaaactcct cgatcgcagc ttcggtcaag ggcccaagcg
    22081 tcgaggagcc atctgcctcg aattccccgc taagctcagc ctcctcggct ggttcataga
    22141 cgaccgcatc gtcctgcgca aagacgcgag tcatgggagc aatcgctacc gtaccagcga
    22201 cagcaagcga aaacgttcgg cgagagatac gaagatcgtt caggtacata cgggtcactc
    22261 ctcaattttg tacgagagca ttcgggcctt cagcagaacc attctgcgtt gcgggccacg
    22321 tgtcactagg gctggcgatg caggctctaa cgcccggctc agacagcttc gaggtcattc
    22381 atggaatacg gtctcttgac aacgcgagtc atgcgagctt cggaggcggc accggtcatc
    22441 ccgtctaacg ggctgtcgtt cgggtcggtt cgtaaccgag gacgaatcga tagccggcgc
    22501 cgtagacggt ccggatgcat tcctggccta tcccggctcc ctcgagcttt gccctcaacc
    22561 aggaaatgtg gacgtccacc gttcgctcct cgacgataat gtcgtcgccc cagacgaggt
    22621 cgagcagttc ctgccggcga aaaacccttc ccggttccat ggcgagcgtt acgaggagcc
    22681 cgaactcttt cggacgcaac cgaagttcac agccattcac ggcggcgcat cgacgatccg
    22741 gccaaatact cagtggccct ctcatcagca ccccggaagg acgggccgat ggaagtgccg
    22801 agcggcgaac cgctgccctt acacgggcga gcaattcgcg aagcgcaaac ggtttgacga
    22861 catagtcgtc ggcgccgagc tcaaatccgt ccaccttgtc ttgctcctcg cctctcgccg
    22921 agagcaaaag gacagcaaca tcgaggtgag cccggatgtt tcggagcacc tgcatgccgc
    22981 tcatccctgg cagcatcacg tcgaggatca ccaggtccag cgacgcgccc tgatccccta
    23041 caacatcgag cgccgcctga ccgctgtctg cggtcagcac ccggtagcct tctcgcgtca
    23101 ggttgaacgc aagcgtgctc cgtagggttg ggtcgtcctc gactatgaag actgtgacgc
    23161 cttcactgtc ggatcgatgg gccgcatcca ccaggtcatc tccaccgcgt cagtcatgag
    23221 actccggaaa ggaactcgaa accgtctaca tcagcgatgg taggggctgg acgttaagag
    23281 ggagtaaggc cagtgttaaa ttgcatccaa tggaatgtaa aatctgtgcc atggccgtta
    23341 acaggagaaa agttccggca gtcgcatcat gcagaacatc gaccctccaa caacgcagca
    23401 aacgttcgga atcaggactc cggcgttgcc gagggtgagg aagtgggatc ctcgggcgca
    23461 tcaatatccg cgtcgccaga ttgcagcatc ccgaccgccg tcgcgagagc cggatcgccg
    23521 cccgtcgagg catgctcttc ggccgtcacc tcaacatctg gttcaatccc ctgttcctgt
    23581 attcggctca tgtcgggggt gaaccactcg gccacggtga cacgcagcgt tccaccgtcg
    23641 tcgagggaga agatgcgctg gaccgacccc ttgccatatg tctgctcgcc tatgacaacc
    23701 gctcgaccgt agtccttcag cgcgccggca acgatttccg cggcgctcgc cgtgccgcgg
    23761 tccacgagca cgaccatcgg gagattggag gacgggctgt tctcatcccg gagaatgggc
    23821 agtggtatct cctctccagc ttccggagag gtgtcttcat acagggcagg gccaacatcc
    23881 ggatcgagaa accgcccgag aacgccttgg gccgaggtca cccagccacc actgttgcca
    23941 cgaagatcga gcacgattcc ggtcgcgcct tgctgctcta acgtccggat cgcatcctcg
    24001 acttctgcca acgtgtcgtt gccgaagatg gtgatgcgga gccagccgat agtcgttccc
    24061 tcgatcatcc gcaaggtgac gggcggcacg acgatctcct cccggaccag cccaagctct
    24121 atggactctc ctgtttctgg ccgcgtcagg gagagagcaa ccgacgttcc cacctcgcca
    24181 cgaagctgcc gcacgatggc atcgaagttc cccggatcga ccggctgatt tccgatgcgc
    24241 tcgatcacgt cgccacgtcg gataccggca tcgtcggcgg gagagtccgc aaccacattc
    24301 gcgaccacaa cggccccctc atcaacggct acatcgatgc ccgtgccgcc gtagcggccc
    24361 tccaggtctt ccagtgccgt cgccgacagc tccggcccga ggtacctggt gtactcgtcc
    24421 tccagcgacg agagcgcgcc gatgatcgcc tgctgctcca tctggcgatc gagccgttcc
    24481 ctctcttctt cagacgtagg acggtagtag tagctttcgt cgataacacc cgcaactgct
    24541 tccaggtcgg gaaatgactg atccgacggc gtggagcccc gtatcacata tcgttcgagt
    24601 accatgcctc cagcgagacc gctgaccaaa atcagcagta ccacggctgc catgcggaag
    24661 taaaaatgca atggaccgag attactgaag ccgggcggcc gggaggaata ccgcgccgga
    24721 ggaaggttgt caggtacgga cgaggtcatg cgagagctcc agctgcggga atggacgatc
    24781 gttcactcga attattgtac gcatgggaga gatgccccct ccccttctcg tggaagacat
    24841 gctcatgctg ctggttgttg acgttggaaa caccaacacg gtgttcggag tctatgactt
    24901 acagtcggac gcgctcagga caaccgtgcg cgcagctacc cgccgggatc gcatgcccga
    24961 tgagtggttt gcgattctgg caccggtcct cgcctccgtc agcattgatc cgcgatctat
    25021 cgaggcaatg gtcatctcga gtgtcgtgcc gagcgtgacg cgctggctct cgacgatgag
    25081 ccaggagcgc ctgaagatca cgccgatcgt ggtcagcgta gatatcgatc tcggtttgcc
    25141 gatcgacatg gacgatccgc gcgaagtggg cgcggaccgg ttggtcaact ccatcgcagc
    25201 gatccaccgc tacggcgcgc cggtgatcgt catcgacttc ggcacggcgc tcaacttcga
    25261 tgttgtcgac gccaggggcc attatatcgg tggttcgctg tcgcccggtc tcgtcattgc
    25321 cctggacgcc atgacatcac gggcggcaag gctcttctcg gtcgatctcg tgcctccgcc
    25381 aggggcgatc ggcagaaaca ccacgcacgc gatccagtct gggctcgtcc tcggctacgt
    25441 tgggctggtg gaagggatga tccagcggat ttcggcggag attgacggca agccaacggt
    25501 tgtggtgaca ggcggttacg cggagctctt cgcgagtcaa tctcccctta tcgacgctca
    25561 cgaccccgat ctcacgatcg acggactgcg gctcgtctac gaacggctaa aggcccgcgc
    25621 ttagtagtag gcatttgggt gacagatttg ccctttccaa ttcaagcgcc atggttcgcg
    25681 atatactttt gcgaatccac acacgaagtt tcgccacgat gtgcctgccg tggagaactg
    25741 tgaaggcaca tctgatctcc aggggagagt acggccatga agcagtatcc gccggaacgt
    25801 atcttcaatg tcggcatgtt ttcgcacggt ggggcgggaa agacgaccct cgtcgaagcc
    25861 atgcttttcg acaccggcgc aacgacccgg cggggacgcg tctcccaggg cacaaccgcg
    25921 tcagacaccg acccggagga gatcaagcgc catatctcgc tcaccaacag cgttatccct
    25981 atcgaataca aggactgcaa gatcaacgtc atcgacacgc ctgatttcgc cgacttcggc
    26041 ggcgatgtcc gttctgccat gcgtgtcgtg gaggcggccc tgatcgtgat cgacgcagcg
    26101 tcaggtgtgg aagtcggcac cgaacaggtt tggcacaatg ccgaacgggc cgggcttcca
    26161 cgcatgatct tcgtcaacaa gatggatcgt gagtatgcca acttccggaa cgtcctcgac
    26221 ggcctgatag aggcattcgg caagcgcgtc gccccattgc agttccccat cggccaggag
    26281 gagaacttcc gcggcatcgt caacctgctg gacaggcagg ccatggtctt ccacgacaac
    26341 cgtgacggcg ggtacgaaac cacggagatt ccggacgagc ttgcggacga tgtcgcgttt
    26401 taccgtcaga ccctcgttga ggcaatcgca gaaaacaacg aagagttgat gatgcgatac
    26461 ctcgacgaag aagagatctc ggacgacgaa ctccggcagg agcttcacca gtgctttgag
    26521 aacggatcgg tgatcccgat ggtgcttggt gccgcctatg agaatcgggg cgtggaaacc
    26581 ctgctcgatg cgatcatcaa cgtcctccct tcagcggccg agcgcaagga aacaggcatg
    26641 gccgacggcg aagccgttga gatcacgccc aatgccgaaa aggacctcgc cgcgctcgcc
    26701 ttcaagaccc aggcggatcc tcatgttggc aaggtaacct ggttccgggt cttcgctggc
    26761 aaaatctcat ccagttcaca gatccacaac gcatccaccg gacaggacga acggataggt
    26821 cagctcttct attcccgcgg caaggaacac atccctaccg aagccgtcac cgcaggtgac
    26881 atcggtgcgg tctcgaagct tgattcggtg gagaccggtc acacgctgac ctcccgcccg
    26941 tcgaacctcc ggctgcatga gattgagttc cccaatgcct cctactcggc ttcaatccac
    27001 ccgcgctccc aggcggatct ggataagttg ggccaggcac ttgctcgaat gcaggaggaa
    27061 gatccctccc ttcgcattga gcgcaacccc gagacaggtg agacgatcat ctccgggatg
    27121 ggagaaccgc acgtccagat tgccgtcgag cggatgaaac ggcgcgccgg catcaacgtt
    27181 gatctcggcc ttccgcgtgt cccgtaccgc gagacaatca cagcaaagac gacggcggag
    27241 tacaagcaca agaagcagag cggaggcgca ggtcaatacg gacacgtgaa gcttgagctt
    27301 gagccgcttc acgatgaaga tttcgcgttc accgagcgcg ttgtcggtgg tgcggtcccc
    27361 cgcaacttct acccggcggt cgagaaaggc gtgcgtgagg cgttggagtc cggacccttg
    27421 ggaggttacc cggtagtcaa cgttcaggcg acgctctttg acggtagcta ccatgacgtt
    27481 gactcgaacg aaatggcgtt caagatcgct gccaaggagg ccttcaagca gggcatcctg
    27541 agcggcaacc ccacgcttct tgagcccatc atggctgttt cgatcagcgt ttcggagaac
    27601 gcgacgggcg acgtcatgag tgatctgaac acccgtcgcg gccaggtgac gggcatggac
    27661 tccggtgata acggcacaac ggtcatcgaa gccgaggtgc cagcaggcga gatccagcgc
    27721 tatgccaccg atctccgatc catcacgcag ggccgtggca cattcacgtc ccgcttcgtg
    27781 cgctacgagc cagttccaca ccatctggcg gaaaagatca tcaccgaggc caagaacaac
    27841 ggtagcaatg gctagtcaaa agtaaccgca ccgacgcgat catgatgtac aacatgaggg
    27901 ggggccgtga gcgaaggcgg ctctctcagg ttcatggtgc acggcgagcg tggcttgggg
    27961 ccgtgcccaa cttgtatggg cggtattcac cgaacctcac cgtgatggca caaacggcac
    28021 ggcaaggcag ttgcaagaat tcaaacaacc aggaattcaa aggagaaccc ctcattgtct
    28081 gattcgaagt tccaacagga atccttaata gagacgacgc ctcccaaatc gcttgcattc
    28141 gagatcgtgt tcgatggcgg ttcgctcggc aacccgggaa agggctatgg cagcttcgag
    28201 atcatgtccg ggggcgagat ttacaagatg ggccgggagg aatataccgg ctcgataacc
    28261 aacaaccagg ccgagtacat gacgctgatc gaatcattga agtggcttgc tgacgacttg
    28321 agcgaggacc ggggcaaggc cacggtcacc atccatgggg acagccagct cgtggtcaat
    28381 cagatcaacg gcacatggaa ggtcaagaac gctcgcatgg tcccgcttgt cgatgaggca
    28441 aagaaactct tcaagcagtt cggcaggtgc acaatcgcct ggcatccacg cgcgaaatcg
    28501 gtcgagcgac tggggcatta gtgctccgtc ccctactcct cacgacgagg tgtcatccgc
    28561 atcgcgacgc tgaaaaagag aacgaatgcg acgacagcga ccaccgctga ggcgacaaag
    28621 acgagaccca ggagcatctt gagcacaacc aaggcgatca acgcagccac aagcgccgag
    28681 aagagcgctt tctcataggc ttgggagcgt gtaggtatgg agtctgttga tcgtcgttgt
    28741 ttcatgaccc ggagtatacc ggcaggagca caacgcgcgt ggcacggaaa agcatctatc
    28801 tcgcctctcc actcgggttc gccgactcta cccgtggatt tatgaacgag ttggtcacgt
    28861 cgctggaagc catcgtggac gttgagaacc cttgggacga tctccgtttc gccggcgagt
    28921 tccaggctgt ccagcgcatt gattccgtga aggagcggcg tgaacgtctc accagggtca
    28981 atacggagat cggccggtgt aacgttgcgc ggattgacgc atccgatggt ctcttcgcga
    29041 ttctcgacgg ggtggatgtc gattctggta cagcagccga gattggctat gcgttcgccg
    29101 cagggaaata tatttgcgga cttcgcaccg atttccggct ggccggcgac aacgaagccg
    29161 cgattgtaaa cctgcaggtg caatacttca tagtggcgag cggtggtcgg gtagcgacct
    29221 cgtcacccga gcttgtcgaa ctcgcgaagg tgttctccgg ctctgagtga ccagcgggaa
    29281 gcccttgcac cgcgttgtcg tggcccctat acttcgcgtg ctgaagggcg attagctcag
    29341 ttggttagag cgccacgttg acatcgtgga ggtcactggt tcgagtccag tatcgcccac
    29401 ccaaggccag ccacctc
//
LOCUS       DASYWM010000266        13089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_162789, whole genome shotgun sequence.
ACCESSION   DASYWM010000266 DASYWM010000000
VERSION     DASYWM010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13089
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162789"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VGR29_10935"
     CDS             <1..311
                     /locus_tag="VGR29_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074142.1"
                     /translation="SFNLFPITMAIGTLALALSAWLRSRGAVIGISFAVMFLMYLFET
                     IGRISPTWAGLRWASAFRFYGSAITDGIDWGNAALLLGVSVVLLVLAIGLFDRRDIYT
                     "
     gene            complement(401..1012)
                     /locus_tag="VGR29_10940"
     CDS             complement(401..1012)
                     /locus_tag="VGR29_10940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2074143.1"
                     /translation="MNQQSTTHINLIRHGETDWNVEHRYQGSTDIPLNDLGRKQVKWL
                     ADSMRDESWDVIYSSPSMRAFDTAKAVAAAIGIDEEAIIRDRRLMERGYGEAEGLTLP
                     EREAKWPEGEWPGLESWEEVAERTMKTLHSAVQDNLGKRILIVCHGGVINAILATISE
                     GEIGTGKTVIDNTSRTTLDVTGDIWTIGDVNNIDHLEALAAAD"
     gene            complement(1133..2713)
                     /gene="gltX"
                     /locus_tag="VGR29_10945"
     CDS             complement(1133..2713)
                     /gene="gltX"
                     /locus_tag="VGR29_10945"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV2074144.1"
                     /translation="MSDTIVARDGMAATSSDRPVSIPEDMAEVRVRFAPSPTGSLHIG
                     GARLVMYNVLFAHGEAPRIGKRGSFILRIEDTDQKRLVEGSVQNLMDGLRWLGLDWDE
                     GPDVGGPYGPYVQSERKHSYKEAAEQLVATGHAYHCFCSPERLQAVREEQQRQKLPPG
                     YDRHCRNLLPDEVQAKLDAGESHVIRFKMPLEGETRFIDLLRGEIVYQNDKIDDLVIL
                     KGDGFPTYHLAVVVDDHEMNITHVTRGPEWIPTAPIHVQLYKAFGWDAPIFAHMPLIL
                     SPTGGKLSKRHGGVSLNDFREAGYLPEALLNYFALLGWSLDGKTEIYSFDDLLEHFTL
                     ERVSASPSTFDRDKLDWLNQQWINHRLTVEDLALRVLPILIRAGMVADGPVDETHPQF
                     AQVKEISGLLKDRIRFLTEAPELMSYFLQDELEDYDASLLVPKKVEPQQALTALEAVQ
                     GMLPELDLSEVEEAEAKLRALADDLGMKAGQLFMPIRVAATGRTQSPGLFETLRTIGQ
                     DRVSTRVDAAVTKLRETA"
     gene            3333..4130
                     /locus_tag="VGR29_10950"
     CDS             3333..4130
                     /locus_tag="VGR29_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2074145.1"
                     /translation="MSVLHRNNVTVMGNGDTPMMFAHGYGCDQNIWRLITLPFIDTHR
                     VILFDHVGAGQSELAAYDRTKYSSLQGYAQDVLEICDELDVKHGIFVGHSVSTMIGVL
                     AAIQEPERFDHLVLIGPSPYYINDGDYVGGFSRKDIQELLELQDSNYLGWSASLAPVI
                     MGNEDRPELAGELRNSFCRTDPEIAKQFAAVTFLSDNRADLPKVPVRSLILQCREDAL
                     APLPVGQYVHRHLPNSELAVLNATGHCPHLSAPDETIAAIQAFLGPT"
     gene            4151..5590
                     /locus_tag="VGR29_10955"
     CDS             4151..5590
                     /locus_tag="VGR29_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2074146.1"
                     /translation="MSDDAFRASLADLYEHGPCGYLFARPDGTILRVNQTLLDWIDFG
                     RSDLVGQRRFQDLLTVAGKVFYENQFFPLLRLQGSVKEVAFDIIRRDREPLPVLLNAV
                     LHTDEEGRPTLIAHAIFDASDRRAFEQELIRSRRSAEQLAAVVRLSSDAILSISAAGI
                     IETWNDGAASMFGFVEQDIVGTNLRDILTPSDRHGAWPDLMEELRAGRPVHTEMVGRS
                     ANGESIDVSAGFTPHRDLLGAVGTVSVIMQNIAERRKVERLQQEFLAVTTHELRSPVT
                     GIKGNAQLMKRRGTYSERFADAIIGQAQRLERLIDDLLLASQIQADRLTLALEVIDLV
                     GAARAAVDYLGVDESAVRVEVPEETLLVYADQHRLSQLLTNLLTNAIKYSPKRADVTL
                     RVTRQGDEAHVSVADRGIGIPPEALPHLFDRFYRVAGTARHVQGLGLGLYISRRIAEA
                     HGGRIDVASEPGLGSSFTVRLPLHHASAE"
     gene            complement(6034..8217)
                     /locus_tag="VGR29_10960"
     CDS             complement(6034..8217)
                     /locus_tag="VGR29_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074147.1"
                     /translation="MRNLLGRLNTRSSDDLVRIARFWQVSLPGTDRGRHVGALYRVMT
                     DIRACRTAWSRFDSTSQDIIRTLASSEAGPRTVAEITEQLGLPGKAVRGGAVQLFRLG
                     VLSIQGDNQEIPVGESPRLFLPRELGQDFRRILDEIDAGDQSGEPLRTLLEARDDADL
                     EETAILWGIKVIPGLRRRQELVGDILQQVSTKRQADAVVASLKRPARALWDVVREQGV
                     EGPVSFVEAVDAAGLTVPDATDIRAVVAGTILRDALIDLETSALVLHTYLDDGSRALF
                     VPREILNPGTVATAVPLRALQPLDPETVPEPEQLHPFALAWDLLTVVREITDRGAPVW
                     VPGEPLSLTWQRKLNRRLWFGREEVPPDGYLGMLLYLALGAGVLEPGPPVSGVGSDKN
                     AVRPVPTSQVRHWRGRSFGQQTAALRDVWLVADQWIEGREREQIDVWGADWQGFRRRL
                     LGALREIEGDEWFLASDVAGRIAEQNPGLIGSTFTAASARGGGRRGDARTAVIAQIIE
                     VELQTAMHWLGFVDLSRIAKKGLAMLVTDAARLAAGDSRAVPELPEPASDEPVLTVDD
                     AGLVVLHRPAPVHIWSLTAFGDAEGLRPEATFQLRPGSVGRALGAGFDLEQIMTYLEH
                     QGGKPLPNGLVNLLREWTAGYKRVRMRRAAVLTPDFETGIDELRTIVKEAKLDVIDPG
                     DVPPDGLVVLLPATGEDSATAEETLQSALRAAGYAGQWATDKRTQ"
     gene            complement(8343..8822)
                     /locus_tag="VGR29_10965"
     CDS             complement(8343..8822)
                     /locus_tag="VGR29_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074148.1"
                     /translation="MPSNNSNAKSVEHRETYRHKRNDTRQNDTRHPEAEELDTLNEIA
                     SPGNEREDATTAGVLRPDETPAHFTDSGDQWTVDVGIDVVGSCGHKIGEVVDVRDDYL
                     VVEKGFFMPEDVFVPKSAIADIDPHHLTLNVTKNTADHSHWDEDPEEPTPQRPPAAS"
     gene            complement(9175..10029)
                     /locus_tag="VGR29_10970"
     CDS             complement(9175..10029)
                     /locus_tag="VGR29_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074149.1"
                     /translation="MFRRGFDMVSAEFFLLRCRMATWVLLTVWTLLGLMFGYIVPYVM
                     YRSDDGRPNMLEAMLPEQITQTLLDGFPFFGGAIALILGVLAVGSEFGWDTLKTLFTQ
                     RPGRGVVFTAKMAALAIMLVPFVVPLFASGAIAGVMIARLEGVDIFWPDAMTVIQGML
                     AGWLVLAVWASVGVLLAVATRGTSLAIGFGILYTLVIEGLISALAGTVSLLEPIVDLF
                     LRANTYSLVRPLGGAGSGTGDSAATDGPGAFSGPYVDELQALVVLVAYMVVFIGVSAW
                     LLRRRDVA"
     gene            complement(10022..11038)
                     /locus_tag="VGR29_10975"
     CDS             complement(10022..11038)
                     /locus_tag="VGR29_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2074150.1"
                     /translation="MKDRVNKRSGFSPGRYVIETDQLTKRYGDIVAVGHLDLRVRQGE
                     VYGFLGANGAGKTTTLRMLLGLIQPTSGKASVLGAKPGTEESLMGVGSMIETPAFYPH
                     LSGRHNLVMLAKYTGTPLKRIDQVLAQVDLSKRADDAFQTYSLGMKQRLGVAAALLKD
                     PVLLVLDEPTNGLDPAGMADMRALIRRLGSGDRTVLISSHLMTEIEQICDRVGVIAGG
                     SLVAEGTVDELRGKDRLLIRVDQIEDARRIVAGLVGEQSVAVSSEVLQVTASNQGEIE
                     SAWLNARLVAQGIAVQELRWDRASLESVFLDLTSGQLRQGKRGQSVIHANGQDQESRE
                     VAHV"
     gene            complement(11059..11325)
                     /locus_tag="VGR29_10980"
     CDS             complement(11059..11325)
                     /locus_tag="VGR29_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEV2074151.1"
                     /translation="MTALNLLQPAAIHAFANDGPGEWWWLWRMLMFLLWIVVIALVAR
                     WIIRGRHHELSPFDRARGILAERYARGEIDAEEYRQRSDQLTNR"
     gene            11612..12307
                     /locus_tag="VGR29_10985"
     CDS             11612..12307
                     /locus_tag="VGR29_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2074152.1"
                     /translation="MEQPTTVLVVDDEDDIVALMRDFLEAEGYRVLTAANGHDALATL
                     GESSIDCVLLDVMMPGPSGFDVLRKIRERSQVPVLFLSARQEDSDKIRGLGLGGDDYI
                     VKSSTPGEIVARVKAVLRRYHQGGVNSANLLDFGRLVVDLRAREAWVNGKQVPLTARE
                     FELLQMFVEHPRQVLTRDTLFETLWGPYGDRHSLTVHIGRLREKIEADPSKPDYITTV
                     WGVGYRFEGAPRR"
     gene            12304..>13089
                     /locus_tag="VGR29_10990"
     CDS             12304..>13089
                     /locus_tag="VGR29_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEV2074153.1"
                     /translation="MSTPRRPRTMSIRRWLSLALAALFLAPMLTLTAIGFFVFRDQEG
                     PWELHQDVREQLIEDAGQWEDPEWQAATREELAQDGADVILLVGGEEVFCTRPDPFAG
                     TDGSPREVRTIDVPGADPPRQAHIFTTASTGPPEELREWFVPIALLTALIGTLAAIAW
                     FLRHSIILPLTAASDAARRISAGELDISLPASRVREVADLNQAFESMSDGLRGSLQRQ
                     VAMERDRRLFISAIAHDLRTPLFSLRGSLEGLEKGIATTPEKRD"
BASE COUNT         2484 a         4122 c         3992 g         2491 t
ORIGIN      
        1 cgtcgttcaa tctcttcccg atcaccatgg caatcggcac tcttgcactc gcgctttcag
       61 cctggcttcg cagtcgaggt gcggtgatcg gcatttcgtt cgcggtcatg ttcctgatgt
      121 atttgttcga aacgatcgga aggatctcac cgacctgggc aggactccgc tgggcgtcgg
      181 catttcggtt ctatggaagc gcaatcaccg acgggatcga ttggggaaac gcggccctct
      241 tgctgggtgt ctccgtcgtg ctcctggttc tggcaatcgg gctcttcgat cgacgggaca
      301 tctacaccta ggatcgcggg ccgtaagacg cgacagtgct actgcaagtc atcccgtaca
      361 gtgagcggga gaacctttgt cgacgttcat gccattggct ctagtcggcg gcggccaggg
      421 cttcgagatg atcgatgtta ttcacatcac cgatcgtcca gatatcgccg gtgacgtcga
      481 gcgtcgtccg gctggtgttg tcgatcaccg tcttgccggt accaatctcg ccctcggaga
      541 tcgtcgccag gatcgcgttg atcacaccgc cgtggcagac aataagaatg cgcttgccaa
      601 ggttgtcctg gacggcggag tggagggtct tcatcgtgcg ctccgccacc tcttcccagc
      661 tctccaggcc cggccattca ccttccggcc atttcgcctc gcgctccgga agcgtcaggc
      721 cctcggcctc tccgtaaccg cgctccatca agcgacgatc cctgatgatc gcctcctcat
      781 cgatgccaat agcggcagcg actgctttgg cggtatcgaa ggcgcgcatc gacggactgc
      841 tgtagatgac gtcccacgac tcatcgcgca tggagtcggc aagccatttc acctgctttc
      901 gtccgaggtc gttgagcggg atatcggttg agccttgata ccgatgctcg acattccagt
      961 ccgtctcgcc gtgccgaatg aggttgatgt gtgttgtgct ttgctgattc acaggcgaaa
     1021 gtaatcctcc aacggggtta cagggatgac ccgcaagcgg gagtgcatgc gacattcggc
     1081 catttgcgag atgaccgcag gcgccgcctg gcgctaagcg gctgctacaa actcacgcag
     1141 tctcacgcag ttttgtgacc gcggcatcga cccgtgtgct cacccggtcc tgaccgattg
     1201 tgcggagcgt ctcaaagagc cccggagact gggtgcggcc cgtcgcggca acgcggattg
     1261 gcatgaagag ctgcccggcc ttcataccga gatcgtcggc aagcgctctc agcttcgcct
     1321 cggcctcctc cacctcggag agatcgagtt ccggcaacat cccctgcacc gcttcaagcg
     1381 cggtcagggc ctgctgtggc tccaccttct tcggcaccag caatgaggca tcatagtctt
     1441 ccagctcatc ctgcaggaag taggacatga gctccggtgc ctcggtgagg aacctgatgc
     1501 ggtccttgag caggccagag atctccttca cctgtgcgaa ctgcggatgc gtctcatcga
     1561 ccgggccgtc cgcaaccata ccggccctta tcaggatcgg cagtacgcgg agggcgagat
     1621 cttccaccgt gaggcgatgg ttgatccatt gctggttcag ccagtcgagc ttgtcccgat
     1681 caaaggtgct tggcgacgcg gagacgcgct ccagtgtgaa atgctccagc aagtcgtcga
     1741 acgagtagat ctccgtcttg ccgtccaggc tccagcccag aagcgcgaaa tagttgagca
     1801 gcgcttctgg caggtagcca gcctcccgga agtcgttcag ggacacgcca ccatggcgct
     1861 tggagagctt gccaccggtc ggcgagagaa tgagtggcat atgggcgaag atcggtgcat
     1921 cccagccgaa tgccttgtag agctgcacgt ggatcggcgc ggtcgggatc cactccgggc
     1981 cgcgggtcac gtgggtgata ttcatctcgt gatcgtcgac gacaaccgcg aggtggtagg
     2041 tcgggaagcc gtcacccttc aggatgacca aatcgtcgat cttgtcgttc tggtagacga
     2101 tctcgccgcg gagcaagtcg atgaagcggg tctccccctc gagcggcatc ttgaagcgga
     2161 tgacgtggct ctcgcccgcg tcgagctttg cctggacctc gtcggggaga aggttccggc
     2221 agtgccggtc gtatcccggg ggcagcttct ggcgctgctg ctcctcgcga acggcctgca
     2281 accgttccgg ggagcagaag cagtggtagg cgtggccagt tgcgaccagc tgctcggcag
     2341 cttccttgta ggagtgcttt cgctcgctct gcacgtatgg cccatagggg ccaccgacat
     2401 ccggtccttc atcccaatcg agaccgagcc agcgaagacc gtccataagg ttctgaaccg
     2461 agccctcgac cagtcgcttc tgatccgtgt cctcgatgcg gaggatgaac gagccgcgct
     2521 tgccgatccg gggtgcctcc ccgtgcgcga agagcacgtt gtacatgacg aggcgggcac
     2581 caccgatgtg cagcgatccg gtcgggctcg gcgcgaaacg gacgcgaacc tccgccatat
     2641 cctcggggat gctgaccgga cgatccgacg atgtcgcagc catgccgtca cgcgcaacga
     2701 tagtgtctga catgcaggga tgttcctttg atgatgggtg tcatacaggt gcggtcgtgg
     2761 cgatgcctgc cgcgttgtta cggttaaaag tatacccggg cctcaaggcg gggcgtgtta
     2821 tccgatgaga tgtagcagac gacgcatagg tgatggggta attgcctatc cgtgaggaag
     2881 atgccgccga tgcgcttcgc gtagggttca tcgccctccg ggcaaatagg cagagccgca
     2941 ggctttgagc gtgagcggcc catctgaatg acgattgcag gctgagggtg agcccgcagg
     3001 gcggcaaaaa acggctcgct gacgcttctc gctggtgctg ccacctcggc caggggctcg
     3061 ctgacgcttc tcgccttgca ggcttaatcg gcgaggtgaa cccctggttg agctcagagt
     3121 gacggaggat ggccagtgct tcagtgacat gctgactgtc atcagcgtct ttgcccgggt
     3181 tcgttgtcgg cttgtcgcga tcgagtcctc cgatttggct ggcccgctgg cgggaacgga
     3241 ccggcatgtt ccgccccggt tcgataagat tcgtatgtac gggtcagcga cagccaccat
     3301 gtcgggtacg tccaggctgg cggggagaaa ccatgtccgt cttgcacagg aacaacgtca
     3361 ccgtcatggg aaacggcgac acacccatga tgtttgccca cgggtatggt tgcgaccaga
     3421 acatatggcg cctgatcacg ctgccattca tcgacaccca ccgcgtgatc ctcttcgatc
     3481 atgtcggtgc gggacaatcc gagcttgccg catacgaccg cacgaagtac agctccttgc
     3541 agggctatgc gcaggacgtg ctggagatct gcgatgaact ggacgtgaag cacggtatct
     3601 tcgtcgggca ctcggtcagc accatgatcg gcgtgctggc cgcaatccag gaaccggagc
     3661 gattcgatca tctggtgctc atcggcccgt caccgtatta catcaacgat ggtgactatg
     3721 tcgggggctt cagccggaag gacatacagg agttactcga actccaggac agcaattacc
     3781 tgggttggtc ggccagtttg gcgccggtca tcatgggcaa cgaggaccgg ccggagctcg
     3841 caggtgagct tcggaacagc ttttgccgta ccgacccgga gatcgcgaag cagtttgcgg
     3901 ctgtgacctt cctgtccgac aatcgggccg accttccgaa ggtgccagtc cgctcgctca
     3961 tcctgcagtg ccgggaagac gcccttgccc cactgcccgt cggccagtac gttcatcgcc
     4021 atctcccgaa ttccgagctg gccgtcctca acgcgaccgg gcattgccct cacctcagcg
     4081 cgccggacga gacaatcgct gcaatccagg cattcctcgg accgacatga tgttggatga
     4141 cagcagcagc gtgagcgacg atgccttccg agcgagcctc gcagatctct atgagcacgg
     4201 accctgcggc taccttttcg cccgtccgga tggcacgatc cttcgcgtga accagaccct
     4261 gctcgactgg atcgactttg gccgtagcga cctcgtgggc cagcgacgtt tccaggacct
     4321 tctcaccgtc gcggggaagg ttttctacga gaaccagttc tttccccttc tccgattgca
     4381 ggggtctgtc aaggaggtcg cgttcgatat cattcgacgc gaccgtgagc cgctgccggt
     4441 gctgttgaac gctgtcctgc acaccgacga ggaaggccgc ccgaccctga tagcacatgc
     4501 tatcttcgac gcatccgacc gacgggcatt cgagcaggaa ctgatacgat cccggcgaag
     4561 cgccgagcag ctggcggccg tcgtcaggct gtcgagtgac gcgatactca gcatctccgc
     4621 ggcgggcatt atcgagacct ggaatgacgg ggctgcctcg atgttcggat tcgtggagca
     4681 ggacatcgta ggtacgaatc tgcgcgacat cctgacaccg tcggacaggc acggtgcatg
     4741 gcccgatctc atggaagaac tgcgggcagg acgcccggtc cacacggaaa tggtgggacg
     4801 gagcgcgaac ggcgagagca ttgatgtctc cgccggcttc acaccgcacc gtgatcttct
     4861 gggcgccgtt gggacggtgt cggtgatcat gcagaatatt gccgaacgcc ggaaggtcga
     4921 gcgattgcag caggaattcc ttgcggtgac gacccacgag ctacgctctc ccgtgacggg
     4981 catcaaggga aacgcccagc tcatgaaacg gcgtggcacc tatagcgagc ggttcgccga
     5041 cgcgatcatc ggccaggcac agcggctcga acggctgatc gacgacctgc tgctcgcctc
     5101 gcaaatccag gccgatcggc tcacactcgc cttggaggtg atcgatttgg tgggcgcagc
     5161 gcgcgccgcg gtggactacc tgggtgtgga tgagtcagca gtccgtgtcg aggtgcccga
     5221 ggagaccctg ctggtgtatg ccgaccagca tcgcctcagc cagctgctga ccaacctgct
     5281 gacgaacgcg atcaagtact caccgaagag ggcggatgtg accctccgcg tcactcggca
     5341 aggagacgag gcacacgtgt cggtggcgga tcgaggcatt ggcatcccac ccgaagccct
     5401 gccccacctc ttcgaccgct tttaccgggt ggcaggtacc gctcgccatg tgcaggggct
     5461 cggtttgggc ctttatatct cccggcgcat tgcggaggcc cacggcgggc gcatcgatgt
     5521 tgcatccgaa ccagggctcg ggagctcctt cacggtccgg ctcccactgc accatgcatc
     5581 tgctgaataa gcgatgacca tgtggtcgga tggcggggtg ccttccgtcg cggtcgcacg
     5641 actggcgctg ggcatgtcga cgacgggcca acgccaccgt gatggaggag tggtcacgcc
     5701 gttcactcac ctccccagga tgcacttctg ccagcagcag caacgatgct caggcgtggc
     5761 gcagaccgtg caccgtaata cagggtacat ccgcagcctt cacgcccggt tgcacatggg
     5821 ccggtctacc catgagggag aggccgccaa tgcgcttcgc gtaggattca tcgccctccg
     5881 ggctctgaat gacgggtctt ggatggatga agcagatgag atgaagcaac gagcccttgc
     5941 tttgcccaga atgacgggtt ctggatgaat gacgtggatg agatggggta acgagtgctg
     6001 gcggtccgga gctatcagcg cggatgatcc gccctactgc gtgcgtttgt ccgtcgccca
     6061 ctggccggcg tagccggcgg cacggagtgc ggactggagc gtctcctcgg cggtggcgga
     6121 gtcctctccg gtcgcaggca ggagaacgac caaaccgtcc ggtggcacat caccggggtc
     6181 aatgacgtcc agtttcgctt ccttgacgat cgtgcgcagc tcatcgatgc cggtttcgaa
     6241 atccggtgtc aacacggcag cccggcgcat ccgaacgcgc ttgtatcctg ccgtccactc
     6301 acgcaggaga ttgaccagcc catttgggag gggcttcccg ccctgatgct cgagataggt
     6361 catgatctgc tcgaggtcga atccggcacc gagcgcccgc ccaaccgatc ccggccgcag
     6421 ctggaaggtt gcctccggcc gtagaccctc ggcatcgcca aaggcggtca gcgaccagat
     6481 atgcacgggc gccggacgat gcaacacgac caatccggca tcatccacgg tgagcaccgg
     6541 ttcatcactg gcaggctccg gcagctccgg aacggcgcgg gaatccccgg cggcgagacg
     6601 ggcggcatcg gtgacgagca tcgcgaggcc ctttttcgcg atgcgggaga gatcgacaaa
     6661 acccagccaa tgcatcgccg tctgcagctc cacctcgatg atctgcgcaa tgactgccgt
     6721 acgtgcgtct ccgcggcgcc caccgccacg cgccgatgcc gctgtgaagg ttgagccaat
     6781 cagcccaggg ttctgctccg cgatgcgccc ggcgacatcg ctggcgagga accactcgtc
     6841 accctcgatc tctcgcaagg cgccaaggag acggcggcgg aacccctgcc agtcggcgcc
     6901 ccaaacgtcg atctgctcgc gctcccgccc ctcaatccat tgatcagcca cgagccaaac
     6961 gtccctcagg gcagcggtct gctgcccgaa gcttcggccc cgccagtgcc ggacctggct
     7021 ggttggcact ggccgtaccg cgttcttgtc gctccccacc ccggaaacag gtggaccggg
     7081 ctcaagcacg ccggctccga gtgccaggta gagcagcatc ccgagatatc catcgggcgg
     7141 aacctcctcc cgtccaaacc agagacggcg gttgagcttc cgttgccatg tcagcgagag
     7201 cggctcgccg ggcacccaaa caggcgctcc cctgtcggtg atctcccgca caacagtcag
     7261 gagatcccac gccaacgcga agggatgtaa ctgctccggc tcgggcaccg tctccgggtc
     7321 cagtggctga agtgcgcgca atggcactgc cgtcgcgacc gttccgggat tcaggatctc
     7381 ccggggcaca aaaagcgcgc ggctgccatc gtccaaatag gtgtgcagta ccaacgccga
     7441 ggtttccaga tcgatgagcg catcccggag gatagtgccc gctactacag ccctgatgtc
     7501 cgtcgcatcc gggacggtga gacccgccgc atccacggct tcgacgaacg aaaccggtcc
     7561 ttccaccccc tgctcccgca cgacatccca cagggcgcgc gccggacgtt tgagggatgc
     7621 gacaacagcg tccgcctgcc gcttcgtgct cacctgctgg agaatatcgc cgacaagctc
     7681 ttgcctgcgt cgcaagccgg ggatgacctt gatgccccag agtatcgcgg tctcctcaag
     7741 atccgcatcg tcccgcgcct caagcaaggt gcggagcggc tcgccacttt gatcgcccgc
     7801 gtcgatctcg tccagaatcc gacgaaaatc ctgcccaagc tcacgtggca aaaagagccg
     7861 gggactttcg ccaaccggga tttcctgatt gtcgccctgt atggagagca cgcccagacg
     7921 aaagagctgg accgcacccc ctcgcacggc tttccctggc aatccaagtt gttcggtaat
     7981 ctcggcaacg gtgcggggac cagcttccga gctggcaagc gtgcggatga tgtcctgcga
     8041 ggtggagtcg aaccgggacc aggcggtgcg gcaggcgcga atgtctgtca tgacccggta
     8101 aagggcaccg acatgacgac cacgatctgt cccagggagg gaaacctgcc aaaagcgggc
     8161 gatccgcacc aggtcatcgg atgatctggt gttgaggcga ccgagcaagt tccgcatggg
     8221 gcgactccgg agatgcttcg ccgctgcagg cagtcctcag gtgaggtggc gtcctgcagt
     8281 tccgccacct cactcctgtg gtaagcctat cagacacctt gcgccgcaac cccggggagg
     8341 gatcagctcg ccgcaggtgg gcgttgcgga gtcggctcct cgggatcttc atcccaatga
     8401 gaatggtcgg ccgtgttctt ggtgacgttc aacgtcaggt ggtgcgggtc gatgtcggcg
     8461 atggcgctct tcggaacgaa gacgtcctcc ggcatgaaga agcccttttc gacgaccaga
     8521 taatcgtcac ggacatccac gacctcgccg atcttgtgac cgcatgagcc gacgacatct
     8581 atcccgacat caacggtcca ctgatctccg gaatcagtaa agtgggccgg ggtttcgtct
     8641 ggccgaagca ctccggcggt ggttgcatcc tctcgctcgt tcccgggaga ggcgatctcg
     8701 ttgagcgtgt ccaactcttc tgcttcgggg tggcgtgtgt cgttttgacg cgtatcgttc
     8761 ctcttatgcc gatacgtttc acggtgttca acggatttgg cattggagtt gttgctaggc
     8821 atgagtgacc tcgtccgctg catcgccacg ggtcaagaat cattgcggac cacctccgct
     8881 ggtgcgtcca ttcctggaag acacacaacg gcccgatgtc gtgcagcagc gcctagtatg
     8941 tgacgattgc atgcaaaatt ccactctccc gtaccatgcc catccctcaa tagcttcccg
     9001 tggtccggca ccggtaaggc gatcgatgag agacggacgc agtcgaggaa cccttgtgct
     9061 ggctctgcaa tccgtttgcc ggctgcgagg attcctcgaa aaggctggaa tgacaggttc
     9121 cctggcgacc acgttgaggt ccaatgcgat gcgcaggccc ggcattggag tcccctatgc
     9181 gacatccctc ctccgtagca gccatgcaga aacgccaatg aacaccacca tgtaggcgac
     9241 cagcactacc agcgcctgca actcatcgac gtagggaccg gagaacgcgc caggaccgtc
     9301 tgtcgccgcg ctgtcaccgg tccctgaacc ggcacctcca agcggccgca ccagtgagta
     9361 cgtgtttgca cgcaggaaga ggtcgacgat tggttcaagc aggctcaccg tgcccgccag
     9421 cgcactaatc agtccttcga tcaccagtgt gtagaggatg ccgaacccaa tcgccagcga
     9481 ggtgccgcgc gtcgccactg caagcagcac gccgaccgac gcccacaccg cgaggacaag
     9541 ccaccctgca agcatcccct ggatcaccgt cattgcatcc ggccagaaga tgtcgacgcc
     9601 ctcaaggcgg gcgatcatta caccggcgat cgcaccggag gcgaagaggg ggacaacgaa
     9661 gggcaccagc atgatcgcca gggcggccat cttcgccgtg aaaactacgc cgcgcccggg
     9721 gcgctgcgtg aacagcgtct tcaatgtgtc ccagccgaac tcgctgccca ccgcgagcac
     9781 gccgaggatc agcgcgatcg cgccaccgaa gaacggaaag ccgtcaagca gcgtctgcgt
     9841 gatctgctcg ggcagcatgg cctccaacat gttgggacgg ccatcatcac tgcgatacat
     9901 cacgtatgga acgatgtagc cgaacatcag gccgagcagg gtccatacgg tcagcaggac
     9961 ccatgttgcc atgcggcagc gcagcaggaa aaactccgcc gacaccatat cgaaacctct
    10021 cctaaacatg agccacctcc ctgctctcct gatcttggcc attggcgtgg atcactgatt
    10081 gcccgcgttt cccctgtcgc agttggccag aggtcagatc gaggaagacg gattcaagcg
    10141 atgcgcgatc ccagcgaagc tcctgcaccg cgatcccctg cgccaccaga cgtgcattaa
    10201 gccaggctga ctcgatctcc ccctggttgc tggccgtgac ctgcaggacc tcactgctga
    10261 cagccaccga ttgctccccg acgagcccag ccacgatccg gcgagcgtcc tcgatctgat
    10321 caacacggat gagcagcctg tccttcccgc gcagttcatc caccgtgccc tccgccacga
    10381 ggctgccacc cgcgatcacg ccaacgcggt cgcagatctg ctcgatctcc gtcatcaggt
    10441 ggctggagat caggacagtc cgatcgccgg agccgagtct ccggatgagt gcccgcatgt
    10501 cggccatgcc agcggggtcg agcccgtttg tgggttcgtc gaggaccagc aacactgggt
    10561 ccttcaagag ggccgccgcg acaccaagcc gctgcttcat cccaagtgaa tacgtttgaa
    10621 aggcatcatc ggcacgtttg gacagatcga cctgggcaag cacctgatcg atccgcttca
    10681 acggtgtacc ggtgtacttc gcgagcatta ccaggttgtg cctgccggaa agatgtgggt
    10741 aaaaggccgg tgtctcgatc atcgacccga cgcccatgag gctctcctcg gtgccaggct
    10801 tcgcgcctag caccgaggcc ttgccactag tgggttgaat gagcccgagc agcatcctga
    10861 gcgtcgtggt cttcccggcc ccgttggcgc cgagaaatcc gtagacctct ccctgccgaa
    10921 cgcgcaggtc gagatgaccg accgcgacga tgtcgccata ccgcttggtc agttgatccg
    10981 tttcgatcac gtacctgcca ggactgaaac cggatctctt gttcacgcga tctttcatca
    11041 caatctctcc catcgtcttc aacggttcgt cagttggtcg ctccgctggc gatactcttc
    11101 ggcatcgatc tcgcctcgcg catagcgctc ggcgagaatg ccgcgtgccc gatcgaacgg
    11161 cgacaattcg tggtgcctgc cacggataat ccagcgagcg acgagagcaa tcacgacgat
    11221 ccagagcaag aacatcaaca tcctccagag ccaccaccat tcgcccggtc cgtcattggc
    11281 aaaggcatga atagcggctg gttgcaacag attgagtgcg gtcatggaac tcactccctt
    11341 gtcggtgcct ttggcacatg aacttgatac agacggcgca catggacgtt ggctggcgcc
    11401 gcgctccttc ggccgtccgg cgtgatccgc caccggccaa ttgccaacac cttgatggta
    11461 ggcagtagag atcacgtccc ggtgggtggt tcctcaacag aacctcaacg gtcagtcacc
    11521 gcttcctcat tccagcctcc aggggctcga tatcctttgc gatgggcaca atactggaga
    11581 gcaggaagat ccggtcaggg aggagcagcc catggaacag cccacgaccg tgctggtggt
    11641 cgacgatgag gacgatatcg tcgccctgat gcgtgatttc ctggaggcag aagggtaccg
    11701 ggtactgacc gcggccaacg gacatgacgc gcttgccacg cttggggaat cgtcgatcga
    11761 ctgcgtgctc ctcgatgtga tgatgcccgg gccatcgggg ttcgacgtgc tccgcaagat
    11821 ccgcgagcgc agccaggttc cggtgctctt tctcagtgcg cgtcaggaag acagcgacaa
    11881 gatccgaggg ctggggctcg gtggggatga ctacatcgtg aagtcatcca cccccgggga
    11941 aatcgtggcg cgggtgaaag cggtcttgcg gcgatatcac cagggtggtg tgaactccgc
    12001 taacctgctg gactttggcc gactcgtggt ggatcttcgc gcccgcgaag catgggtaaa
    12061 cggaaagcag gtcccattga ccgcgcgcga gttcgagctg ctgcagatgt tcgtcgagca
    12121 tccgcgacag gtcctgaccc gcgacacgct gttcgaaacg ctgtggggtc catatggaga
    12181 ccgtcactcc ctcacggtgc acatcgggcg cctgcgcgag aagatcgagg cggacccatc
    12241 gaagcccgat tacatcacca ccgtttgggg cgtgggttat cgcttcgagg gagcgccgcg
    12301 ccggtgagca ctccacgacg cccccgaaca atgtcgatcc gccggtggct gtcacttgcc
    12361 ctggcggcac ttttcttggc gccgatgctg acccttaccg ccatcgggtt cttcgtcttc
    12421 cgtgaccagg agggaccgtg ggagcttcac caggatgtca gggagcaact gatcgaagat
    12481 gcaggccaat gggaggaccc ggagtggcaa gccgcgacac gtgaagaatt ggcgcaagat
    12541 ggcgcggacg tcatcctgct ggtgggtggg gaggaggttt tctgcacccg tcccgatccc
    12601 tttgcgggca cggacggttc tccccgcgag gtccgcacga ttgacgttcc cggcgccgat
    12661 ccaccccggc aagcccatat cttcacaacc gcgtcgactg gtcctccgga agaattgagg
    12721 gagtggtttg tcccgatcgc gttgctcacc gcactgatcg ggacattggc ggccatcgcg
    12781 tggtttcttc ggcactccat catcctgccc cttaccgcgg caagcgatgc cgcacggcga
    12841 atttcggcag gggaactgga catctcgtta ccggcatcga gggtccgcga ggtcgcggac
    12901 ctcaaccagg ccttcgaatc gatgagcgat gggcttcgtg ggtcacttca gcgccaggtt
    12961 gccatggagc gcgaccgtcg cctcttcatc agcgcaatcg cgcatgatct ccgcacgccg
    13021 ctcttctcgc tccggggttc actggaaggg ctggagaagg gaatcgccac gacacccgag
    13081 aagcgggac
//
LOCUS       DASYWM010000267         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_669152, whole genome shotgun sequence.
ACCESSION   DASYWM010000267 DASYWM010000000
VERSION     DASYWM010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669152"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1161)
                     /locus_tag="VGR29_10995"
     CDS             complement(67..1161)
                     /locus_tag="VGR29_10995"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074154.1"
                     /translation="MRTRFRHIVSPLIGALSSVAAVIVALLLGAIVIRASGGDPVEAY
                     RALFKGAFEGRRALADTLIYTAPLILGGLAFAIAARAGMFNIGIEGQLVVGGFAAGLV
                     GAWDLGWPTFLYVPLALLAAMIAGGFWGFIPGFLKAKTGAHEVITTIMLNYLAFRLIT
                     YLTLRQSGFLPVDPTSVATERIVDAARLPNILEGTRLHAGIIIALISAVALWFILYRT
                     TFGYKVRAVGLSPGASAYGGINWGAIMMVAMALSGLLAGLAGAGESIGLHGRHWSTPP
                     GYGYTSIAVGLVGRNNPLAVILSGLLFGALSAGAPAMQSADVSKEIIFVIQGLIILAV
                     AALNVMGRLPALRSRMVKRLANRRALRAGI"
     gene            complement(1133..2695)
                     /locus_tag="VGR29_11000"
     CDS             complement(1133..2695)
                     /locus_tag="VGR29_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2074155.1"
                     /translation="MTEAAPTATAPFAIEMRDIVKRFPGVVANDGITLQVRQGEIHAL
                     LGENGAGKSTLMNILFGLYAPDEGQVFVNDKPANFHGPREAVHAGLGMVHQHFMLIPR
                     FTVTENVILGAEGPVTMLDRKSAARRVAGIARSYGLDIDPDARVEDISVGLQQRVEIL
                     RALYQGSRMLILDEPTALLTPQEIEDLYGVLDRLRAAGGTIIFITHKLREVAAVSDRV
                     TVIRRGKTIGTRETKGTTPQELAEMMVGRGVSLTVERQPAAPGETILEARNLQARSDH
                     GHRALDIESLRLRRGEILGVCGVEGNGQGELVEILSGLREWSSGSLLIGGHDVKDRDP
                     NGFRDAGLSYIPEDRHHRGLVLDFPLWENVLLGNTEREPFVRGGMIDSRASQAITRSL
                     MEKYDVRAPSPTTQARNLSGGNQQKLIIARELQRNPDVLLAIQPTRGLDVGAIEFVHK
                     ALIEVRDAGKAVLLVSYDLDEILDLSDRIVVLYQGRITGEFLSGQVSRSKLGLFMGGR
                     TTNDADAIPTHS"
     gene            complement(2751..>3503)
                     /locus_tag="VGR29_11005"
     CDS             complement(2751..>3503)
                     /locus_tag="VGR29_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2074156.1"
                     /translation="APQYPDKYFAIIDTVVEGDNVVSYVFRENEGSFLAGVLAGLMTK
                     TGVIGYVGGIRVPPVMRYEVGYVAGARSVNPDIEILISYSDDFEDPALGKELTLAQYN
                     NGADIVLAAAGRTGIGSFDAAKEKGEGFFVIAADQDQSELGAEFQLAAVLKKLDTAVY
                     DSIEMVLNDSFESGAHDIGIAEDGVGIGFFHESVPQDVKDTIEAYRAAIGEGTIEPPA
                     DDEGLADFTPVPPAEVGGSASPAASPEASPAA"
BASE COUNT          710 a         1077 c         1079 g          637 t
ORIGIN      
        1 aagagtagtg gcgtggtcat ccttagaatg gccgtcagga gtgcgaggga gaaaaagtcc
       61 gtcatgtcag atcccagcgc gcagggcgcg acggttagcc agtcgtttca ccatgcgact
      121 gcggagagcg gggagccgcc ccatcacatt gagtgcggca acggcgagaa tgatcaggcc
      181 ctggatgaca aagatgatct ccttggaaac atcggccgat tgcatggcgg gagctccagc
      241 actcagcgct ccgaagagca ggcccgaaag gatgactgcg agtggattat tgcggccgac
      301 aaggccaact gcgatggagg tatacccata tcccggggga gtgctccagt gccgcccatg
      361 caggccgatg gattcgcccg cgccggcaag cccggcaagg aggccggaca gggccatggc
      421 gaccatcatg atcgcgcccc aattgatgcc gccgtatgct gacgctcctg gtgaaaggcc
      481 gaccgcccga accttgtacc cgaacgtcgt gcggtagagg atgaaccaga gggctaccgc
      541 ggagatgagg gcgatgatga tgccagcatg gagccgggtc ccctcaagga tgttcggcag
      601 gcgggcggca tcaacgatcc gttctgttgc gacggacgtc ggatcaacag gcagaaaccc
      661 gctctgtctc agggtgagat aggtaatcag cctgaacgcc agataattga gcatgatcgt
      721 tgtgatcacc tcatgggcgc ccgtcttcgc tttcaggaat ccagggataa atccccagaa
      781 cccgccggca atcatcgcgg caagcaatgc cagcggcacg tacaggaagg tgggccagcc
      841 gagatcccac gcgcccacca gtccagcagc aaatccgccg actaccaact ggccttcgat
      901 cccgatgttg aacatgccgg cccgggctgc aatcgcgaac gcgagcccac cgagaatcaa
      961 cggcgcggtg tagatgaggg tgtcagcaag ggcccggcgt ccctcgaagg ctcccttgaa
     1021 gagcgcccgg taggcttcca ccgggtcgcc gcccgacgct cgaatgacga tcgcgcccag
     1081 caggagcgca acgatcaccg cggcgaccga gctcaacgcg ccgatcagcg ggctaactat
     1141 gtgtcggaat cgcgtccgca tcgttggtcg tgcgtcctcc catgaagagt ccgagcttgc
     1201 tgcgtgatac ctgtccactc aggaactcgc cggtgatcct gccctgatac agcacgacga
     1261 tgcgatcgct caggtccaga atctcgtcaa ggtcgtacga gaccagcaac actgccttac
     1321 cagcgtctcg tacctcgatc agtgccttgt ggacgaactc gatggcgccg acgtcaagac
     1381 cgcgggtcgg ctggatagcc agcaggacat ctggattgcg ttgcagctcg cgcgcgataa
     1441 tcagcttctg ctgattaccg cctgaaagat tccttgcctg cgtcgtcggc gatggtgccc
     1501 ggacatcgta cttctccatc agggagcggg tgatggcctg ggacgcgcga ctgtcgatca
     1561 taccgccccg gacaaacggc tcgcgttccg tgttgccaag aagcacgttc tcccagagtg
     1621 gaaaatctag gacgagcccg cggtgatgcc ggtcctcggg gatgtaggag agcccggcat
     1681 cgcggaatcc gttgggatcg cggtccttca catcgtgacc gccgatcagc aacgagccgg
     1741 aggaccattc ccggagaccg gagaggattt ccaccagctc gccctggcca ttcccctcca
     1801 cgccgcagac gccgagaatc tcacctcgac gcagcctgag cgactcgata tcgagggctc
     1861 tgtggccgtg gtcgctcctc gcctgaagat ttcgcgcctc aaggattgtc tcgccaggtg
     1921 ccgccggttg tcgctcgacg gtgagcgaga caccacgacc gaccatcatc tcggccagtt
     1981 cttgcggggt tgttcccttc gtctcacggg tgccgatggt cttgccccgc cggatgacgg
     2041 tcacacgatc gctgacagcg gcgacctcgc gcagcttgtg cgtgatgaag atgatcgtgc
     2101 ccccagctgc acggagacga tcgaggaccc cataaagatc ctcgatttcc tgtggggtca
     2161 ggagggcggt aggctcatcg agaatcagca tacggctgcc ctgatagagg gcacgcagga
     2221 tctcaacgcg ctgctggaga ccgacggaga tatcctcgac acgtgcgtcc ggatcgatat
     2281 cgagcccgta ggacctggcg atcccggcaa cccgcctggc ggccgacttc cggtcgagca
     2341 tcgttactgg accctcggca ccgagaatga cattctctgt cacggtaaac ctagggatca
     2401 gcatgaagtg ctggtgaacc atgccgagcc ccgcatgcac agcctctctt ggtccgtgga
     2461 agttcgcagg tttgtcgttg acgaacacct gtccttcgtc gggcgcgtac agaccgaaga
     2521 ggatgttcat cagcgtcgat tttcccgcgc cgttctctcc gagcagcgcg tggatctctc
     2581 cctgccggac ctggagggtg atgccgtcat tcgccacaac cccgggaaat cgcttcacga
     2641 tatcccgcat ctcgatggca aatggtgcag tggcggtggg tgcagcttca gtcatgggca
     2701 cagatagagg agggcacgga tggatgatcc gtgccctcgg cactcaccta ttaggcggct
     2761 ggcgaggctt ccggcgatgc ggccggggaa gcgctgccac ccacctcggc tggaggaacg
     2821 ggcgtgaaat ctgccaggcc ctcgtcatcg gctggtggct caatagtgcc ctcgccgatc
     2881 gctgcccggt acgcttcaat cgtgtccttc acgtcctgcg ggacactctc gtggaagaag
     2941 ccgatgccga caccgtcctc ggcaatgccg atatcgtgcg caccgctctc gaagctgtcg
     3001 ttcaacacca tctcgatgga gtcgtagacc gctgtatcaa gcttcttcag gacggcggcg
     3061 agctggaact cggcaccaag ctccgactga tcttggtccg cggcgatgac gaaaaagccc
     3121 tcacccttct ccttggcggc atcgaacgag ccgatgcctg tgcggcccgc ggcagcaagg
     3181 acaatatcgg caccgttgtt gtactgggcc agggtgagct ccttgcccag cgccggatcc
     3241 tcgaagtcat cggagtagga gatgaggatc tcgatgtccg ggttgacgga acgggcgcca
     3301 gcgacgtagc cgacttcata gcgcatcacg gggggtacgc ggatgccgcc aacgtagccg
     3361 atgacaccgg tcttcgtcat aaggccggcg agtacgccag ccaggaacga accttcgttc
     3421 tcacggaaga cgtaggagac gacgttgtcg ccttcaacca ctgtatcgat aatcgcaaaa
     3481 tacttgtccg gatactgtgg agc
//
LOCUS       DASYWM010000268         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_87472, whole genome shotgun sequence.
ACCESSION   DASYWM010000268 DASYWM010000000
VERSION     DASYWM010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87472"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VGR29_11010"
     CDS             complement(<1..387)
                     /locus_tag="VGR29_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV2074157.1"
                     /translation="MTSTFERYHDAASRLDALIDMTPTPEDTSREAVQRRADVRMRRL
                     VRFLERLGNPHQGSPIIHIGGTSGKGSTSTALAMILTEAGYRTGLHTSPYLQTPLEKL
                     QLDGRLIDPAVFAGIVDELLSEHERWM"
     gene            526..1230
                     /locus_tag="VGR29_11015"
     CDS             526..1230
                     /locus_tag="VGR29_11015"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEV2074158.1"
                     /translation="MLSLLLNIIAPTVIMMRFASEDKLGPVTALLVALAFPFVYGMYG
                     MIRERKIGWMPIVGLVSILISGGVGLLKLPAEWIAVKEATIPLILAFAIIISSWIGKP
                     LARIFLDQILDKERVYKALRERGAFEEYERRTSIATWLLASAFFLSAVLNFILATIVV
                     TADGGTEEYTEQIGRMTALSFPVITIPVFIVLTGTILYIMKTVSTLTGLESEDVLRNP
                     KKEVLKESSDEERVTQ"
     gene            complement(1398..>2252)
                     /locus_tag="VGR29_11020"
     CDS             complement(1398..>2252)
                     /locus_tag="VGR29_11020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HEV2074159.1"
                     /translation="NGLDAERLAGYRAAGVNRLSIGAQTLDRRGLRTLGRQHEAVDVL
                     DAVRMAHSAGFERINLDFIFGWPGQQREAWQADLERVLALGDEGPDHLSLYSLIVEPG
                     TPYADAAARGILQVPDDDAAADLYEDAIAILDAAGWDHYEIANWARTPERYSRHNAIY
                     WRHGDYLGIGAGAYGTIGGIRVMNHMLPETYIRAIEGGTLPHSNSEVIDEKTGRGETM
                     MLGLRLLDDGIQKAAFSQRHGVELETHFGTQIAQMEKIGLLERVDTGVRLTPRGMMLA
                     NDVAAAFL"
BASE COUNT          398 a          718 c          674 g          462 t
ORIGIN      
        1 catccacctt tcgtgctcgg agagcagctc gtcgacaatc ccggcgaaga ccgcagggtc
       61 gatcaaccgt ccgtcgagct gaagcttttc gagcggtgtc tgcaggtaag gtgaggtgtg
      121 gagaccagtg cggtaaccgg cctccgtgag gatcatcgcc agggccgtgc ttgtcgagcc
      181 cttgccggag gttcccccga tatgaatgat ggggctcccc tggtgcggat tccccaaccg
      241 ctcaagaaag cggaccagtc gacgcatgcg cacatctgcc cgccgctgta ctgcctcgcg
      301 cgacgtatcc tccggggtcg gagtcatatc gatcagggca tccaaccggg acgccgcatc
      361 gtggtatcgt tcgaatgtgc tggtcatgtc ccatcccgcg atgtcaccat cgcaccatgc
      421 gcgtcgttcg gcacgcgtgg aagcgctgac cacatgtgcc accgcgtgta tggtacgcgc
      481 aagggaaccg ggtgtgatcg atcttcaggg atacaggact cattgatgct cagtctgctg
      541 ctcaacatca ttgctccgac cgttatcatg atgcgcttcg ccagtgagga taagctcggc
      601 ccggtgaccg cgctgctggt ggcgctggct ttcccctttg tctacggcat gtacgggatg
      661 atcagggagc gcaagatcgg gtggatgccg atcgtcggcc ttgtctcgat cctgatctcc
      721 ggcggcgtcg ggctgctgaa gctcccggcc gagtggatcg ctgtcaagga agccaccatc
      781 ccactgatcc tcgcattcgc gatcatcatc tcatcctgga ttggcaagcc tctcgcccgg
      841 atattcctcg accagatact cgacaaggaa cgcgtttaca aggccctgcg tgagcgtggc
      901 gcctttgaag agtacgagcg ccgcacctcg attgccacgt ggcttctcgc cagtgcgttt
      961 ttcctgtcgg ccgttctgaa cttcatcctg gcgacaatcg tcgtcaccgc cgatggcggt
     1021 accgaggagt acaccgagca gatcggcagg atgaccgccc ttagcttccc ggtgatcacc
     1081 atcccggtct tcatcgtgct cacgggaacg atcctctaca tcatgaagac ggtcagcacg
     1141 ctcacaggcc ttgagtcgga ggacgtgttg cgcaacccga agaaggaagt tctcaaggag
     1201 tcctcagacg aggagcgggt gacccagtag gatcacgatc cagggtaccg gcgaattcta
     1261 tcccggctcc ttgccgctga ttggggcgaa ctccatcttc gcccgtaagc ctggttggat
     1321 cgcctgcagg tgctgggctc aattgctttt gttctcctga gagttccgcg catggcccag
     1381 cgctcgaacg agtcgtccta gaggaacgcc gccgccacgt cgtttgccag catcatcccg
     1441 cgcggcgtca accggacgcc ggtatccacc cgctccagaa ggccgatctt ctccatctgc
     1501 gcgatctggg ttccaaagtg cgtttccagc tcgactccat gccgttggga gaaggccgcc
     1561 ttctggattc cgtcgtccag caatcggagg ccgagcatca ttgtctctcc ccggccggtc
     1621 ttctcgtcga tgacctcgct gttggagtgg gggagggtgc cgccctcaat tgcccggatg
     1681 tacgtctccg gcagcatgtg gttcattacg cgtatcccgc cgatggtgcc gtaggcgcca
     1741 gcgccgatgc cgagatagtc accgtgtcgc cagtagatcg cgttgtggcg ggagtatcgc
     1801 tccggcgtcc gagcccagtt cgcaatctcg taatgatccc aaccagctgc gtcgagtatt
     1861 gcgatcgcat cctcgtacaa atccgccgcc gcgtcatcgt ccggcacctg aaggatgccg
     1921 cgtgccgccg catcggcata gggcgtcccg ggttcgacga ttaggctgta gagcgagaga
     1981 tggtccgggc cctcgtcacc gagggctagc acgcgctcga gatccgcttg ccaggcctcc
     2041 cgctgctggc ccggccagcc gaagatgaaa tccaggttga tgcgctcgaa accggcggaa
     2101 tgcgccatcc ggaccgcatc aaggacatcg accgcctcat gttgacgtcc gagagtgcgc
     2161 agtccccgcc ggtcaagcgt ctgcgcaccg atgctcaggc ggttgacacc ggcagcccgg
     2221 tatccggcca accgttcggc gtccaggcca tt
//
LOCUS       DASYWM010000269         4634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_331769, whole genome shotgun sequence.
ACCESSION   DASYWM010000269 DASYWM010000000
VERSION     DASYWM010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331769"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VGR29_11025"
     CDS             complement(<1..526)
                     /locus_tag="VGR29_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2074160.1"
                     /translation="MAELFKNFIGGQWIESHTGRTFERKNPATGELIGTYTNSDAKDV
                     DAAVSAASRAFKSWRLYPAPKRGEILFRAAQILEQRKEALAREMTEEMGKVLDETRGD
                     VQEAIDMTYYMAGEGRRLFGETTPSEMRSKFQMSVRQPLGVVGLITPWNFPIAIPSWK
                     MMPALIAGNTVIIKP"
     gene            complement(616..687)
                     /locus_tag="VGR29_11030"
     tRNA            complement(616..687)
                     /locus_tag="VGR29_11030"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(653..655),aa:Asn,seq:gtt)
     gene            860..1897
                     /locus_tag="VGR29_11035"
     CDS             860..1897
                     /locus_tag="VGR29_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074161.1"
                     /translation="MWDPRSYEDTDYGLEPGESFEPDAPWEAGDEEVSTAGPHIMTVL
                     GPIDPQDLGICLVHEHLLRQPTPATAWEPDHWLDNEGLISEEIEAFVAMGGRSIVDCS
                     TSDHGRDAAGLVHLAMRAPVHIIGVTGRYNGMRSSRGEDTIDVERLTAEFVADLLDGM
                     GETSARAGMLKIATGRDQITELEVASIQAAAQAHSATGAPVTISTEADTTGLERLHLL
                     TRAGMDLSHVIVGRLDEKLEWDHLVAAAETGAFIAFDGVSKPGHTSDTDRASMLVRLA
                     ESGYADRLLISHGHDRRSSYISYGGQPGITYILERFSLELMDAGAEALLVRNMLIENP
                     ARALTIHPPAC"
     gene            1946..2182
                     /locus_tag="VGR29_11040"
     CDS             1946..2182
                     /locus_tag="VGR29_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074162.1"
                     /translation="MPIRLFLTSARVLDGPAEEGDIPVDRFFVNASEVSEVWVDTEST
                     ITPERGKSATFLLIRNLDLGFTRITGTIERKVQK"
     gene            complement(2357..3184)
                     /locus_tag="VGR29_11045"
     CDS             complement(2357..3184)
                     /locus_tag="VGR29_11045"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEV2074163.1"
                     /translation="MTVPTIYLEQVAALALADQISEWLKERLDEAHADRFVLGLSGGI
                     DSAVVAGLCARAAGPERVLAVMMPSASNPDDLACAMEVAETFGLKSTTIDLTSVADTF
                     YASVPDVATVFSGVLGEEPGNGIEERSQLAKANVRPRLRMLTLYYLANLARGIVVGTG
                     NKTELMIGYFTKYGDGGVDIFPLTDLYKFEVRAVARAIGVPERVITRPPSAGLWEGQT
                     DEQEIGLTYDELDSTLSAMDTGHASLLDSEVHARVQHMVSTTAHKRAPVPTFSRKRP"
     gene            complement(3257..>4634)
                     /gene="lpdA"
                     /locus_tag="VGR29_11050"
     CDS             complement(3257..>4634)
                     /gene="lpdA"
                     /locus_tag="VGR29_11050"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV2074164.1"
                     /translation="LGGGTGGYVAAIRAKQLGLNVAVVERFKIGGVCLHQGCIPTKAM
                     LKSAGVFDTIKHAGDYGVDIKGDISFNYDNALGRSLKIVDGQYKGLQYLFDKKHKIPV
                     FLGTGVLTANDTIEVTLTQGGEKVRLKARDIIINTGSRPRAIEGIEFDGKRVINSDHA
                     VVLEKMPKSIIVRGGGATGVEWASIYHRYGSKVTLVGRIVPNEDKEVSQQLTRAFQRQ
                     KIDMYVDVRPTKDDFEISDKGVKMTIKDKKGKETVVEAEMLLVATGRQANIENIGLET
                     VGVKTEKGFIAVDSMMRTNVEHIYAIGDVNGQQMLAHTAQHHGIIAVEHMVGENPFPL
                     DVLNSPSCTYCEPEIGSVGLTEQAAKDLGYEVLIGKFPMRPNAKAVIEGDPEGFTKVV
                     VDADTKDVLGVHIIGAHATELIAEAGLARFLNASVEELFMTVHPHPTVSEVIGQAAMD
                     ALGHAIDI"
BASE COUNT          901 a         1358 c         1369 g         1006 t
ORIGIN      
        1 ccggtttgat gatgacagtg tttccagcga taagagccgg catcatcttc caggaaggta
       61 tggcgatcgg gaagttccac ggcgtgatga gcccgaccac accaagtggc tggcgcacgg
      121 acatctgaaa tttgctccgc atttccgatg gtgttgtttc cccgaatagg cgtcggccct
      181 cgcctgccat gtagtaggtc atgtcgatgg cttcctggac atccccgcgc gtttcgtcca
      241 ggaccttacc catttcctcg gtcatctcgc gagcaagcgc ttccttgcgc tgctccagga
      301 tctgcgccgc gcggaagagt atttcgccac gcttcggtgc cgggtagagg cgccatgatt
      361 tgaatgccct ggaggctgcg gagacggccg catcgacatc cttcgcgtcg ctgttcgtgt
      421 aggtcccgat gagctccccg gtcgccgggt tcttgcgttc aaacgttcga ccggtgtggg
      481 attcgatcca ctggccacca atgaaattct tgaacaattc ggccatgtcg ccttcctttc
      541 agagcgtggc tcgcgcctgc caacttttgg actctagcaa aaaggcactg ccggaaaccc
      601 ggcagtgcct gatagctccc cgaagaggac tcgaacctct aaccctccgg ttaacagccg
      661 gatgctctac cattgagcta tcggggatcg ggctccttgc gagcaacgac ctgattttac
      721 cacaactcca cctcccttca aggccgtatt cgacctgcga tgtagctgct gtcagagacg
      781 tccgttcagg ctttttactc cctcaccctg ctaggtatga ggaggttggc gggaatgcag
      841 acgggaaggc aatggcaccg tgtgggaccc acgatcatac gaggatacgg actacgggct
      901 cgagccgggt gaaagtttcg agccagacgc cccatgggaa gccggtgacg aggaggtgag
      961 cacagccggt cctcacatta tgacggtgct tggcccgatc gatcctcagg acctcggtat
     1021 ctgcctggtg cacgagcact tgctgaggca gccgaccccg gcaacggcct gggaaccgga
     1081 tcactggctc gacaacgaag gacttatctc cgaggagatt gaggcgttcg tggcgatggg
     1141 aggtcgctcg atcgttgatt gctcaacttc agatcacgga agggatgctg ccggtctggt
     1201 gcatcttgcg atgcgggcgc cggtacatat cattggcgtt accggacggt acaacggcat
     1261 gcgctcttcc cgtggcgagg acaccatcga tgtggaacgc cttacggcgg agttcgtcgc
     1321 ggatctgctc gacggaatgg gggagaccag tgcgcgagcc ggcatgctga aaattgctac
     1381 gggtcgcgac caaatcacag agttggaagt ggcgtccata caggctgcag cgcaggccca
     1441 ttcggcgacg ggcgcgccgg tgacgatcag cacggaggcc gacacgacgg ggctcgagcg
     1501 gctacacctt ttgacaagag ccggcatgga tctgtcccat gtcattgttg gtcgtctgga
     1561 tgagaagctg gaatgggatc atttggtggc ggctgctgag acaggtgcgt tcatcgcgtt
     1621 cgatggggtg agcaagccgg gtcacacttc cgataccgat cgtgcctcga tgctcgtccg
     1681 gctggccgaa tctggctatg ccgatcggtt gcttatctct catggtcacg atcggagatc
     1741 ttcatacatc tcgtatggag ggcagccggg aatcacctac atactggagc gtttctcgct
     1801 tgaactgatg gacgccggcg ccgaggctct gctggtgcga aacatgctta ttgagaatcc
     1861 ggcacgggcc ctcacgatcc atccgccagc ctgctgacaa ccagcaacgt cgtgttgtgc
     1921 gaacagaaac tggagggtat cactcatgcc gatcaggctc tttctcactt ccgcgagagt
     1981 ccttgatggt cccgcagaag agggggatat ccccgtcgat cgcttcttcg ttaacgcatc
     2041 cgaagtatca gaggtgtggg tggataccga gtcgacgatc accccggagc gagggaagtc
     2101 ggcgaccttt ctcctgatac gcaaccttga tctcggtttc accagaatca ccggaactat
     2161 cgagcgcaag gtgcagaagt aggcctggac acagggccgc gtcggcgtac gtcgctgcgg
     2221 tgtggattct ttcacgagac aaatcgggag tgcccgaaga accccttttg ttttccaagg
     2281 ttacatcata tgagcaagaa aaggtgcaga tatcaatacc tgcacctttt cttactcgtc
     2341 gcacgttgct tgtctgttag ggccgcttac ggctgaaggt cgggactggc gcccgcttgt
     2401 gagcagtcgt gctcaccatg tgctggaccc tggcgtgaac ttcggaatcc aggagggacg
     2461 cgtggcctgt gtccatggca ctgagtgtgg agtccagttc atcataggtc agaccgattt
     2521 cctgctcgtc ggtctggcct tcccaaagtc ccgccgacgg cggcctcgta atgacccttt
     2581 ccggtacgcc tatcgctcgc gccacagctc gcacctcgaa tttgtacaga tcggtcaacg
     2641 ggaagatgtc gacaccacca tctccgtact tggtgaagta accgatcatc aactcggtct
     2701 tgtttcccgt gccgacgacg attccccgcg cgaggttggc gagatagtac aacgtcaaca
     2761 tgcgaagcct cggccggacg ttcgctttgg caagctggga ccgttcctcg atcccgtttc
     2821 ccggctcttc gccaagaaca cccgaaaaga ccgtcgctac gtctggaaca gacgcataga
     2881 aggtatcagc aacggaggtc agatcgattg tcgtcgactt gagcccgaat gtctcggcga
     2941 cctccatcgc gcaagcgagg tcgtcggggt tggaggcgga aggcatcatg acggcgagca
     3001 cccgctccgg ccccgcagcg cgggcacaga gcccggcaac cacagcggaa tcaatgccgc
     3061 cgctgagccc gagcacgaac cgatcggcat gcgcctcgtc gagtcgctcc ttgagccatt
     3121 cgctgatttg gtcggcgagc gcgagtgccg cgacctgctc aagatagatg gttggtaccg
     3181 tcatcacatt gtttccttcg acgggtgatt gggtcggccg accgcgattc gagaacggcc
     3241 gatgtcctgg gcttcgctag atatcgatcg cgtggccaag ggcatccatg gctgcctgac
     3301 cgatgacctc tgatacggtc gggtgcgggt gaacggtcat gaagagttcc tccacgctgg
     3361 cgttgaggaa gcgggcaaga ccagcttcag ctatcaactc ggtcgcgtgc gcgccgatga
     3421 tatgcactcc aagaacgtcc ttggtgtcgg catcgaccac gaccttggtg aatccctccg
     3481 ggtctccctc aatgaccgcc ttggcattcg gacgcatcgg gaacttccca atgagaacct
     3541 cgtatccgag gtcctttgca gcctgctcgg tgagaccgac ggatccgatc tccggctcgc
     3601 aataggtgca gcttggggag ttgagcacat ccagcgggaa gggattctcg ccgaccatgt
     3661 gctccacagc gatgatcccg tggtgctggg ctgtgtgagc gagcatctgc tggccattga
     3721 cgtccccgat tgcgtagatg tgctcaacat tcgtccgcat catcgagtca acggcgatga
     3781 agcccttctc ggtcttcaca cccacggtct ccaagccaat gttctcgatg ttcgcctggc
     3841 gcccggtcgc gacgagcagc atttctgcct caacgacggt ttccttgccc ttcttgtcct
     3901 tgatcgtcat cttgacgccc ttgtcggaaa tctcgaagtc atccttcgtc gggcgtacgt
     3961 cgacgtacat gtcgatcttc tggcgctgga aggcgcgcgt cagttgctgg ctgacttcct
     4021 tgtcctcgtt cggaacaatc cggccaacga gagtgacctt cgaaccataa cgatggtaga
     4081 tgctcgccca ctcaacaccg gtggcaccac cgccgcggac gatgatgctc ttcggcatct
     4141 tctccaggac gacggcgtga tcgctgttga taacccgctt tccgtcgaac tcgatgcctt
     4201 caatggcacg cggccgcgag ccggtgttga tgatgatgtc cctggccttg agccggacct
     4261 tctccccacc ctgggtgagg gtcacctcaa tggtgtcatt cgccgttagc actccggtgc
     4321 ccaggaaaac agggatctta tgcttcttgt cgaagaggta ctggagaccc ttgtactggc
     4381 catccacgat cttgagagag cggccgaggg cgttgtcgta gttgaaggag atatcaccct
     4441 taatgtcaac accgtagtcg ccagcatgct tgatggtgtc aaagacaccg gcagacttga
     4501 gcatggcctt ggtggggatg cagccctggt ggagacagac gcctccgatc ttgaaacgtt
     4561 cgaccacggc tacgttcagt ccgagctgtt tcgcccgaat ggcagccaca tagccgccgg
     4621 tgccaccgcc gagg
//
LOCUS       DASYWM010000270         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_425820, whole genome shotgun sequence.
ACCESSION   DASYWM010000270 DASYWM010000000
VERSION     DASYWM010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425820"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..489
                     /locus_tag="VGR29_11055"
     CDS             34..489
                     /locus_tag="VGR29_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV2074165.1"
                     /translation="MSSKHHSADLAMQHMSLFRRIIEEGFTNQEFDVFDQIISPEILE
                     HQPGMGAGPQGVKGAVAYLHSVFPNFTLAIEELTYDGEKVWARLRARGTQEGSHMGMP
                     PTGKPIDVTVFDICRFQDGKMVEHWGVPDRFAIMEQLGMLPVPGPPQAA"
     gene            complement(580..2001)
                     /gene="odhB"
                     /locus_tag="VGR29_11060"
     CDS             complement(580..2001)
                     /gene="odhB"
                     /locus_tag="VGR29_11060"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEV2074166.1"
                     /translation="MTPGSEIAKGQIGRYVQETETGMAIEIKVPALGESLVDAVVGQW
                     LKGEGDSVSQGETILELETDKVNLDVTAESDGVLSAIQKQEGDVVTVGEILGTLDSDG
                     APAAASANGQEETPAEVEPAEAPVAANVAEARAESKEDNPAGTEPATVAPSGDDSSAG
                     RAAPAVRRMAEEHNIDLAQVDGTGPNGRITRDDLMGHLARLQTKSAQPTPAPDVEPQK
                     AAPPQAPATQPAAAPAPAATAQPDREERVRLSRRRQTIATRLVEAQQTAAMLTTFNEV
                     DMSAVMELRSRRKDAFKEAHGVNLGFMSFFTKAVVGALKAFPEVNSELQGTELVLKKY
                     YDIGIAVGAEEGLVVPVVRDADRKSFAGIEKEIRELATKARDGKLTLPELTGGTFTIT
                     NGGVFGSLLSTPILNTPQVGILGMHTIQQRPVAVDGEVVVRPMMYIALSYDHRVVDGS
                     GAVQFLVKIKQLVEDPETLLLEG"
     gene            complement(2066..>4006)
                     /locus_tag="VGR29_11065"
     CDS             complement(2066..>4006)
                     /locus_tag="VGR29_11065"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEV2074167.1"
                     /translation="ARAVQDNRDHPGQPDQEKTRSLAILIHGDAAFPGQGVVAESLNL
                     SGLAGYNTGGTMHIIVNNQIGYTTDWRDSRSTLYAGDLAKGFEIPVIHVNADDPIACL
                     SAARIATEYRKTFGKDFLIDVVGYRRWGHNEGDEPAFTQPLMYQTITRHPTVRKIWAD
                     DLSRQGVITDDDAAAMEKRIYDRLMKIRQGVTEGTETFEDEAPEEVRPREEVDTALTP
                     DRMRDLQTALHALPEDFSPNGKLKRQWSARAAVLDKEDGKLDWAHAESMAFAAMLQEG
                     TPIRLTGQDAQRGTFSQRHLVLHDAKTGDVYAPLEHIPGATASFAVYNSPLSENACVG
                     FEYGYSVNAPEALVIWEAQFGDFVNGAQVIIDQFLSAARSKWGQNPSLVMLLPHGYEG
                     QGPEHSSARLERFLQLAAQDNIRVANCTTSAQYFHLLRRQAARLQNDPRPLVLMTPKS
                     LLRHPLAGSSPAEFVEGTFRPVLDDPKAADRKDTVTRLILCSGKVAVDLETADAREGA
                     EQVAVARVEQLAPFQNTAITKVIENYPNLKEIVWLQEEPRNMGAWGYMGPRLRDLVED
                     RLPVRYIGRPDQASPAEGFAELHQAAQKAIVHAAFAADLKSDDSTAEEEKVPSGDRLT
                     NGKSEEATAEEGKKKVTVSSAD"
BASE COUNT          727 a         1257 c         1200 g          822 t
ORIGIN      
        1 attatattca tactctaatg aaatgaggat cgcatgtcca gcaagcatca ctctgccgac
       61 ctcgctatgc agcacatgag cctcttccgg aggatcatcg aggaaggctt cacaaaccag
      121 gaattcgatg tcttcgacca aatcatctct ccggagatcc tggagcatca accgggcatg
      181 ggagcgggac ctcagggtgt caagggcgcc gttgcctatc tccactccgt cttccccaat
      241 ttcacactgg ccatcgagga actaacatac gacggcgaga aagtatgggc acgactacga
      301 gcacgtggga cccaggaagg ttcgcacatg ggcatgccgc ctacagggaa gcctatcgac
      361 gtcacggtgt tcgacatctg tcgatttcag gatggaaaga tggtcgagca ctggggtgtc
      421 cctgaccggt tcgccatcat ggaacaactc ggcatgctgc cggtgcccgg gcctccccag
      481 gctgcatagc gccggagtgg caactggatg acagacggca aacccccggc gctactgccg
      541 ggggttcgtt gcatccctga ttgatctgtg caggagggac tagccttcca gaagcaaggt
      601 ctcaggatcc tcgacgagct gcttgatctt gaccaggaac tggacggcgc cactgccgtc
      661 gacgacccgg tgatcgtacg acagggcgat gtacatcatc ggacggacga caacttcgcc
      721 gtcgacggca accggccgct gctggatggt gtgcatgccg agaatgccga cttgtggggt
      781 attgagaatc ggcgtggaga ggagcgagcc gaaaacgcca ccgttggtga tcgtgaacgt
      841 gccgcccgta agctccggca atgtcaattt gccgtctcgc gccttcgtcg ccaactcgcg
      901 aatctccttc tcgataccgg cgaagctctt gcggtccgca tcgcgaacca ccggcacaac
      961 caggccttcc tcggcaccaa cggcgatccc gatgtcgtag tacttcttga ggaccaactc
     1021 ggtgccctgg agctcggagt tgacctcagg gaacgccttc agcgcaccaa ccacggcctt
     1081 cgtgaagaag gacatgaacc cgaggttgac gccgtgcgcc tccttgaagg catccttgcg
     1141 gcgacttcga agttccatca cggccgacat atctacttcg ttgaatgttg tcagcatcgc
     1201 cgcggtttgc tgggcctcga cgaggcgggt cgcgatcgtc tggcgacggc gactgaggcg
     1261 cacgcgctct tcgcggtccg gctgcgccgt cgccgccgga gcaggcgcgg ctgccggctg
     1321 tgtagcgggc gcctgtggcg gggctgcctt ctgcggctca acatccggag ccggggtcgg
     1381 ctgagcgctc ttcgtctgca gcctggcaag gtggcccatc agatcatcac gggtgatgcg
     1441 cccgttggga ccagttccat caacttgcgc cagatcgata ttgtgctcct ccgccatccg
     1501 gcgcactgcg ggggcagcgc gacccgccga agaatcatcc cccgacgggg caacggttgc
     1561 tggctcggta ccggccgggt tgtcttcctt actctcggct ctcgcctctg cgacgttcgc
     1621 ggctaccgga gcttcggccg gttcgacctc cgccggcgtt tcttcctgtc cattcgcgga
     1681 tgcggcagcg ggagcaccgt cagaatccag cgtccccaga atctcaccaa cggtgacgac
     1741 atcgccctcc tgcttctgga ttgcgctcag cacaccgtcc gactcggcgg tcacatcgag
     1801 attgaccttg tcggtctcca gttcaagaat cgtctccccc tggctgacgg agtcgccttc
     1861 acccttcagc cattgaccga ccacggcgtc gaccagagac tctcccaatg cgggaacctt
     1921 gatctcgata gccatccctg tttctgtctc ctgcacatat cggccgatct gccctttggc
     1981 gatctccgat cctggcgtca attcccatac gtccactgag cgcgtgattt ggcatttgcc
     2041 tcctcacgcg ctcacctgtc catgttcagt cggccgacga gaccgtaacc tttttcttgc
     2101 cctcttcagc agtagcctct tcggacttgc cgtttgtcaa gcggtcacca gacgggacct
     2161 tttcttcctc ggctgtgctg tcatcggatt tgaggtcagc ggcaaacgct gcgtgaacga
     2221 tcgccttctg cgccgcctgg tgcagctcgg caaagccttc tgccgggctg gcctgatccg
     2281 gtcgaccaat gtagcgtacc gggagccgat cctcgacaag atcacgcagg cggggcccca
     2341 tgtaacccca ggcgcccata ttacgtggct cttcctgcag ccagacgatc tctttcaaat
     2401 tggggtaatt ctcgatgacc ttagtgatcg ccgtgttctg gaagggggcc agctgctcga
     2461 cccgggcaac agccacctgt tcggcgcctt cacgtgcatc ggcagtctca agatccacag
     2521 cgaccttgcc gctgcagagg atgagacggg taaccgtgtc cttgcggtcc gccgccttcg
     2581 ggtcgtcaag caccggccgg aacgtgccct cgacgaactc agcgggcgac gatcccgcga
     2641 gcggatgccg cagcaggctc ttcggagtca tcaacacgag tggccgtgga tcgttctgga
     2701 gacgggccgc ctgtcgtcgc agcagatgga agtactgggc agaggtcgtg cagttcgcaa
     2761 cacggatatt gtcctgtgcg gcaagttgga ggaaccgctc aagtcgcgca ctggagtgct
     2821 caggcccctg accctcatag ccgtgcggca gcagcatcac cagggaaggg ttctgccccc
     2881 actttgagcg agcggcggag aggaattggt cgatgatgac ctgagcaccg ttcacgaagt
     2941 cgccgaactg tgcttcccag atcacaagcg cctcgggcgc attcacgctg tagccatact
     3001 cgaagcccac gcaggcgttc tcagagagcg ggctgttata gacagcgaag gatgctgtag
     3061 ctccggggat gtgttccagc ggcgcgtaga catcgcccgt ctttgcgtca tgtagcacga
     3121 ggtgtcgctg gctaaatgtg ccacgctggg catcctggcc cgtaagccgg atcggcgtgc
     3181 cctcctggag catcgcggca aacgccatgc tctcggcatg cgcccaatcg agcttgccgt
     3241 cctccttgtc gaggacagca gcgcgtgccg accactggcg cttcagcttg ccattgggag
     3301 agaaatcttc gggcagcgca tgcaaggctg tctgcagatc acgcatgcgg tccggggtta
     3361 gcgcggtgtc gacctcttcc cgaggccgga cctcttcggg ggcctcatcc tcgaaggtct
     3421 ctgtgccctc ggtgacgccc tgccggatct tcatcagccg gtcatagatg cgcttctcca
     3481 ttgcagcggc atcatcgtcg gtgataacac cctgcctgga gagatcatcc gcccagattt
     3541 ttcgcacggt tggatgcctg gtgattgtct ggtacatgag tggctgggtg aatgccggct
     3601 cgtcgccttc attgtggccc cagcgccgat acccgacgac atcgatcagg aaatccttgc
     3661 cgaacgtctt gcgatattcg gtcgcgatcc gggcagcgga gagacaggcg atcgggtcat
     3721 ccgcattgac atggataacc gggatctcga agcccttcgc cagatcgccc gcatagagcg
     3781 tcgaccggga atcgcgccag tcggtcgtat acccgatctg gttgttgacg atgatgtgca
     3841 tggtcccgcc ggtgttgtat cccgcgaggc cggagaggtt gagcgactcg gcgacgacgc
     3901 cctgaccggg gaaagccgca tcgccgtgga tcaggatggc gagagagcgt gttttctcct
     3961 gatccggctg tcccgggtga tcccgattgt cctgtacggc acgagc
//
LOCUS       DASYWM010000271        18916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_632089, whole genome shotgun sequence.
ACCESSION   DASYWM010000271 DASYWM010000000
VERSION     DASYWM010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 18916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18916
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632089"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1898
                     /locus_tag="VGR29_11070"
     CDS             <1..1898
                     /locus_tag="VGR29_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEV2074168.1"
                     /translation="RKVAQRKRELEHSLKGIGQRRADTITSIQSMVVKISPPSPLAGW
                     GWANGDHEDPCAACDRLLVTIDGIDLSVHERTEQNLQTLRTLGHNHVQAQERLATYEQ
                     RRKELDVKLENLLKEGDPAQRIDEIAARMKVLLEREVELRTALTRLAELHEQTMQMVS
                     KLQQQHFDELCPTCMRPFTATDMDVTLQALQSRLRELDGKRRELHRELRVHDQELARL
                     QAEQDQLEKRATELARVRQSIEASIPYISSQRNDVEALSAQLRTTLGQLGRADLPGES
                     DLARASEELQTWRRIVDARATIERSRSSLRLLVTEVSDLDQQVREIGEVHYDEVAHTQ
                     LTKDLREAHKAQTTVTQIDRDLSRRPQLEADLASCRGAIASTSATIAKTEQERVEIGF
                     VAAVLDAAVSAAREARQAENIAIEMRHRVQATLRDAENAHEALIKDQERISLLATDAD
                     ARQREYDRLDQMYREFTEFEKFAAAWYVPRLSEITSELIAEVTDGKYDRVVFDNNFGI
                     DIYDGEEEKFPLETFSGGARDVIALCARIALSRVIGGQGAHPLGFLVLDEVFGSLDRD
                     RRTRLLEMLGAITNSGEHFRQIFMISHVDDVRTAPIFDELWQVIESEEGSSSIHNLAP
                     GADIGDL"
     gene            2601..3971
                     /locus_tag="VGR29_11075"
     CDS             2601..3971
                     /locus_tag="VGR29_11075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2074169.1"
                     /translation="MSTETVRIDPVTTRPGGRRRSRTESLSQALRFPAIVWALHFLLT
                     QIPASLAYRYGEIRSVHRVNGVWQPTDGQESGAYQIIRPSLDGWAHWIVEPFRSWDGT
                     WYSMIAEQSYSTTYSALSAFFPLYPWLMDLGSQLTGLPVETVGYLISHLAFLGALIVA
                     YKLVRLDFNERVARMTLVAVTIFPTTFFFSAIYTESLFFFLSVTCLWAARKNDWLLAG
                     IMGFFAAMTRSAGIMLLAPLAVLFIQQHGWDLRRWFPKALLGLLLPAGLAVFGWFLTT
                     KGLDFLDWKNQQWQWNRFSATPWRTLECTFQSCENVTVRGFGGTYETNVPSVSFAWIG
                     DLWDHLSWSYFTSSEFRYTVGQSQWLDVIVTLLAFGLILLGLKKLPLYYSAWMIPPMI
                     VPLLAPSSVFPLMSMPRFVLPLLPLFVMAVLLIPNRKVGYVLAGTSATLLVLLTTQFA
                     LWYWVA"
     gene            4276..5946
                     /locus_tag="VGR29_11080"
     CDS             4276..5946
                     /locus_tag="VGR29_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV2074170.1"
                     /translation="MVNVQESPAKPSTGSTSMTGGQALISSLLRQGIDTIFALPGVQL
                     DGAFDALYDAQNSGDVRVIHTRHEQGAAYMADGYARATGRIGTCMVVPGPGLLNATAA
                     LSTAYACNQPVLCVTGQIQSNLIEFGRGLLHEIPNQLGMIRSVTKHAARATTPSDIPG
                     LVDTAIRELWTGRVRPVEIEVPPDTLLSTGDVALLGAAPARQRTEADPEKIAEAAKLL
                     GSSTSPIIFVGGGIHGSQAWDELRELAEMLQAPVVMTQNGKGALSDRHYLAQHTFAGK
                     NLLAGADVVLAVGTRFVEPTTYSWRLQEDRTVIQMDIDPEEIGRNYPPTVGVVSDAKV
                     GLAALIEETARHNRSRPSREAELSAIKQAAQEKLDSVEPQAPFAHAIRRQLPDDGIFV
                     GEMTQVAYWSNAGFPVYEPRTYLTPGYQGTLGWGFPTSLGVKVGAPEKVVVSVNGDGG
                     FGFALNELATQAQHNIKSITLVFNDGAFGNVRRIQTEDFRGRTIASELKNPNYVKLAE
                     AFGVAGRRATTPAELETQLKEAIADDEPALIEIPVQPMPNPWKTLALR"
     gene            complement(6007..6264)
                     /locus_tag="VGR29_11085"
     CDS             complement(6007..6264)
                     /locus_tag="VGR29_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074171.1"
                     /translation="MENDGQNGLLGMSEAELYGFLDELLQGEAVEAASMTGNSLEEEL
                     NSPGFAAAAAANSYAIKLIMANNAYITRQLLDQGVLPNGDE"
     gene            6593..6832
                     /locus_tag="VGR29_11090"
     CDS             6593..6832
                     /locus_tag="VGR29_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEV2074172.1"
                     /translation="MTYVIAQPCIGTKDASCVEVCPVDCIHSDDDSEIYHINPAECID
                     CGVCAEVCPVEAIFFEDDLPEQWASFKPRNAAYFE"
     gene            complement(6911..9271)
                     /locus_tag="VGR29_11095"
     CDS             complement(6911..9271)
                     /locus_tag="VGR29_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074173.1"
                     /translation="MTLSAPTAVAFAVSGGTPADVREWITVDFVGQPSPYRRGFAVMT
                     ESLTLSGRGEELARHARALILQELRRHQRQPADAALARAFAVANSIVFEEGRFPGGIG
                     QRYLIGATAIVFEGHQATIGHLPPGQLVLAQDGLVYAVPEIGSWLPHYAETQDDAPTP
                     EPLGYASWTAPVLVQTELREGDTLILCTAKIGEGLAELSARPGGDQLSVSGFHGRDPD
                     KVMDVLREVVIERELPVAAAAVIGFPPAERGSGVETIGDIGRNAREQWRHGRAAIRQF
                     LPVRPSRAARPTVAAGVSAIDMSSGAGDLDDVVRVPAAGWRKPEESIQERLIRLTERR
                     SGDWRDTWRQPSELRQLGAPGAHGISRYRDSSLGAGEPSWKHTMPRLPFLRSPVFIVL
                     CVVALLGVSALLYTERGRFLPSEDDYMAHVAEADRQLLLARDTSNEDIVQDALDDAEA
                     ALADAERAGAPEELLSPRQNEITLERDAIQQVVRLADVTRIGGLPAALQSSRTRAIHT
                     PGGIFLANGSLYRLRPESREMQLVLQSGSDVEGLQVGDLFGVAYDGETLVASDGQHVF
                     FAGSAEGSAWQGMTLEEINQQGPWPPGPIAAFAENIYLLVPEYRNIYQFTLNPEEQRV
                     GPMDWVLTGDRVTLNLAVDMTVDGNIYVLLEDGRVLTMRRGAQVASFDLPVFDPETET
                     PLAIVSGPMTGYLYVAVVDDQGDGRVIAVDREGGHMSQLELPAGFSTGESDVLPPFDD
                     LQDIAVDEASGTLYLVNGDAVWTARYSLPPLPVPAGTPPATPPVPQ"
     gene            complement(9447..11591)
                     /locus_tag="VGR29_11100"
     CDS             complement(9447..11591)
                     /locus_tag="VGR29_11100"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2074174.1"
                     /translation="MVSSWVEKLAGKGRRGGNGVLVQIAREVVGRRRSVQGAISEVRH
                     PAVLDALSDDDFRILDQLIEERVASDREFAQVLARLTHAAAHAKSFDRQTVDSALRLD
                     TLLPADDPAREREKLLRDAYKAAQRAGYVQGGRRALGRLGNRAASAGDTERARVLLQQ
                     QVDLGPENTDTEDEVESEILLGDIILRDGNPEGAMDLYERAGRSAERLGYSRGIAESL
                     IHRLDTGGESLDLETRARMEEDALRVSTDLRDDVILAQLIGQHAHTLLALERYEDAVA
                     HLEDGLEVAREVGDLELENACLAMLSDVEQKIGRFETAVDRQRDLVQVEERLGNMPAA
                     ASDAIHYGSTLLTLGRLQDAREVFEHAAKLAEDAGDTRLRQRAFGGLGVTLSAMNHPV
                     DALNSLMQALELARSTQDVAHEAQWLGSIGEALWKFDQGEDAIQAIRQAIEAARRADD
                     VDLQAGMLSLLGQIHLADRKIRQSVEYYQDALDLYRQLGRRDEEVSVLSQLGTMAMDA
                     NQVGDAIQLYGEALDVAAQSGQRAAAVRLYGRLARLAQRQGDTNAALDALTQAVEIAE
                     TIDQPVLLNQALQHLAVAQDAGNHPDALDTYERALRLSREVGDEYGETLMLTNVGARL
                     LAQGRRRDAVEVLEHGLHLADSLGVVGEKLAERARMLVREARSSGSRTGQRDVATRPM
                     VAQPMGEARTRTPQLAPIAEDLNRGPDQAQVS"
     gene            complement(11585..12580)
                     /locus_tag="VGR29_11105"
     CDS             complement(11585..12580)
                     /locus_tag="VGR29_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEV2074175.1"
                     /translation="MEQQEVWSGPVPTLADVFQARKVVRQYLQPTPAIESPALSERMG
                     CRVVVKCENLQPIGAFKIRGGIYLLSTLSDSERAAGVVAASTGNHGQSIAYAARIFGE
                     HATIFVPNGANPLKVAAMERLGADVRFACDEFNDCHDEAGRWAKAEGKYFIHSANVPQ
                     LIAGVGTSTLELMEEHPDLDAIFVAIGGGSGACGACIAGKGISDRLKVYGVQAEGAPV
                     VYESWRERTLKKIGPAKTFAEGIATSEAFSLPAKILWNQVDDIVLVSDSDLRRSMVTL
                     LETTRMVAEGAGAAGLAGLHQRRNELQGKKVAVVLSGGNLTLETLEKALAEERPW"
     gene            12825..13670
                     /gene="pyrF"
                     /locus_tag="VGR29_11110"
     CDS             12825..13670
                     /gene="pyrF"
                     /locus_tag="VGR29_11110"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV2074176.1"
                     /translation="MPTSLSFQDKLDARIGAADSLLCVGLDPVTARMPQGFDGSPEAV
                     VSFCKEIVSATTDLAAAYKPNLAFFMALGTRGLEALHSVREAIPSDIPVILDGKLNDM
                     GQTAKAYSNAVFDTLGFDALTVAPYLGEDALAPYLEKGGRGVIVLCKTSNPGSGDLQD
                     LELASGELLHLHVADRCQEWDRRYPANVGMVVGATYPPQLAEVRRRCPDQVILLPGLG
                     AQGGDAASSVRAGISSSGGRLLCSASRSIMYAGDTADFAEAARAAAGRLRDEINAHRR
                     ARRSA"
     gene            13816..15084
                     /locus_tag="VGR29_11115"
     CDS             13816..15084
                     /locus_tag="VGR29_11115"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEV2074177.1"
                     /translation="MRTVLHSLVTRVFFTILALTLIAPVLPGMAPQPASANSLAEQLG
                     IDPEYIPPDFGQTALSVYVSATGHSVQGYMLDYWRANGAASVYGNPISEAFGARNGLY
                     SQAFERGIFQFSPEWVWTDDAAVRLMPIGTQEVREDRLATRSDGRRTGADRRANAWRP
                     GSESSVRASEVSNEGGRFSNLTGYSISGAFAAWYDSHEGWFYMGAPISEPHIERGVPV
                     QYFENGILMQQEGVITPAPLPREKPEAYGIDTTPVAQDGRPEFSEALFINNPNPFGVD
                     VTQLSGRKRIVVNIPEQKMWVYQGDTLVLETFVSTGLSPNDTEAGNFHVRIKFETQDM
                     SGFTSDTGEVVGLGDEESAEGTVTANTYEVADVPHVMYINYEAEALHGAYWHNNFGQK
                     MSHGCINQPLDVAAFLYDFAPLGTAVDVIE"
     gene            15192..16115
                     /locus_tag="VGR29_11120"
     CDS             15192..16115
                     /locus_tag="VGR29_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2074178.1"
                     /translation="MGHHRNEPHGLPHWDYATGTPLNDSPTGSRRIAEKAPPSFPVQI
                     VAFANQKGGVGKTTSAINTSVVLASRGYRVLLLDMDPQGNATSSLGIDKQELDASIYD
                     VLVEDASIGECRISDVRPGLDVLPTTSTLAAAEIELVGVSDRERRLARACAPIRHEYD
                     IALIDCPPSLGLLTINSLTAASSVVVPIQCEFLALEGVSQLIHTIDLTRRQLNPTLDL
                     LGVLMTMFDARTRLSAHVVDEVRRFFPERIFETIVPRSVRLAEAPSYGQAISEYDPVS
                     RGGSAYASFANELVARLGLKQPSGTDVNSSR"
     gene            16137..17642
                     /gene="guaB"
                     /locus_tag="VGR29_11125"
     CDS             16137..17642
                     /gene="guaB"
                     /locus_tag="VGR29_11125"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV2074179.1"
                     /translation="MTDPQPTSSPMREDKFAGLGLTFDDVLLIPARSDVIPADVSTRT
                     RIAADIELNIPIVSAAMDTVTEARLAIAIAREGGIGIIHRNLSIAEQADEVDKVKRSE
                     SGMIVEPVTLGPDQPVNDALSVMERYHISGVPITEDDGKLVGILTNRDLRFIQNPDQM
                     ISEVMTRENLITAPVGTTLEEAEEILHHHRVEKLPVVDDNGCLKGLITVKDIQKKIQY
                     PNATKDSQGRLRVGAAIGVGPGSEERAQELIRRGVDLLVVDTAHGHNSGVLNMVRRLK
                     SKHSIAIVAGNIATGAAAEALIDAGADGLKVGIGPGSICTTRVVAGTGVPQITAIHEA
                     AKAAQKHNIPIIGDGGIQTSGDIAKAIAAGADAVMLGSLLAGLDECPGEVIVFKGERF
                     KEYRGMGSLGAMRGRSFSKDRYFQGDIGNVSKLVPEGIEGRVAYKGQLGPLVYQLVGG
                     LRSAMGYAGAPDIATLRNDSQLIQITNAGLAESHPHHVTVTREAPNYRMSS"
     gene            17680..18309
                     /locus_tag="VGR29_11130"
     CDS             17680..18309
                     /locus_tag="VGR29_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV2074180.1"
                     /translation="MRNLVYFVATTIDGYITTASDVDPDFLLYDGPQAANLLQEFPET
                     IPGHLRSLLGVPDETPNQRFDTVLMGRATYDIGRAIGVTSPYPHLRQIVVSSSLGPSP
                     DPAVEVISSDVVSQVHEIKRSNGKDIWLCGGGNLAASIINEIDEFIFKISPVVLGGGV
                     SVFGELVGPRKLTMTSHRVYENGFSLLRYRQVAGSSTNALHPKTSHSAR"
     gene            18272..18802
                     /locus_tag="VGR29_11135"
     CDS             18272..18802
                     /locus_tag="VGR29_11135"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2074181.1"
                     /translation="MPSTQKLLTPPGDEPPRVPTGPHLVRRVPYLAGQFLLYLVMFQI
                     YKMVRMQFIPHDESVAYRHAKDILHLEEQLGLMFELDLQRWVLDQGDWLITFFNNVYA
                     YYMWGFQFGMLFLAFMALPRYRYMRRAFFISMAIATPMYLIYPLAPPRFMEPYGWPFM
                     DTLAVYGRTTFSRPAS"
BASE COUNT         3629 a         6133 c         5472 g         3682 t
ORIGIN      
        1 tgcgtaaggt cgcccagcga aagcgcgagc tggagcactc gctgaagggg atcgggcaac
       61 gtcgagccga tacgatcaca tcaatccagt ctatggtcgt caaaatctcg ccaccctctc
      121 cgctcgccgg atggggatgg gcaaatggag atcatgaaga tccctgtgcc gcatgcgatc
      181 gcctgcttgt gaccattgat gggattgatc tctctgttca cgagcgcacg gagcagaatc
      241 ttcagacact tcgaacgctg ggccacaacc atgtgcaggc gcaagagcgc ctcgctactt
      301 atgaacagcg tcgtaaggaa cttgacgtca agctggagaa ccttctgaaa gaaggcgacc
      361 ccgcgcagag aatcgatgaa atcgcggcgc gcatgaaggt cctcctcgag agagaagttg
      421 agctgcgcac ggcgctcacc cggctggcgg agctgcacga gcagacgatg cagatggtat
      481 cgaagcttca gcagcaacat tttgacgaac tttgcccgac atgcatgcgc cccttcaccg
      541 ccaccgatat ggatgtcacg cttcaggcac tacagagccg tttgcgggaa ctggatggga
      601 agcgccgcga actgcatcga gagctacgcg ttcatgacca ggagcttgca aggctgcagg
      661 cagagcaaga tcaactcgag aagcgcgcca ctgaactcgc cagggtgcgc cagagcatcg
      721 aagcgagcat cccctacatc tcgtcccaac ggaacgacgt ggaggcgctg tcggcacaac
      781 tgcgcacgac gcttgggcaa cttggacggg ccgacctgcc gggggagagc gacctggcgc
      841 gggcaagcga ggagctccag acgtggcgca ggatcgtcga tgcccgcgcc accatcgagc
      901 gctcgcgctc ctccctccgc ctactcgtca cggaggtctc ggacctcgac cagcaagtaa
      961 gggagatcgg cgaggttcac tacgacgagg tggcccacac gcagctcacc aaagacctcc
     1021 gggaggcaca caaggcgcag acaacagtca cccagattga ccgcgacctc tcgcggcgac
     1081 cgcagcttga agcggatctc gcctcctgcc ggggagccat cgcctctacc agtgccacta
     1141 tcgcgaagac cgaacaggag cgagtggaga tcgggtttgt cgccgccgtg ctggatgctg
     1201 cagtgagcgc ggcccgcgaa gcacgtcagg ctgagaacat cgccattgaa atgcgtcaca
     1261 gggtgcaggc aactcttcgc gatgcggaga atgcccatga ggcgctcatc aaggatcagg
     1321 agcgcatctc cctgctggca acggatgcgg acgccagaca gcgggagtac gaccgcctgg
     1381 accagatgta ccgggagttc accgagttcg agaagtttgc ggccgcgtgg tatgtccctc
     1441 gcctgagcga gatcacgagc gagctgatcg ccgaagtgac ggatggcaag tacgaccggg
     1501 tggtgttcga caacaacttc ggaatcgaca tctatgatgg cgaggaggag aaatttccac
     1561 tggaaacctt ctccggtggc gcacgcgacg ttattgccct ctgcgcccgg atcgccctct
     1621 cccgggttat cggcggtcag ggagcccatc cactaggctt cctcgttctc gatgaggtct
     1681 tcggctcgct ggaccgggat cgccgaaccc gcttgctgga gatgctggga gcgattacca
     1741 attccgggga gcacttccgc cagatcttca tgatcagcca tgtcgatgac gtccgcacgg
     1801 caccgatctt cgacgagcta tggcaggtca tcgaatccga ggagggttcg agctccatcc
     1861 acaacctggc acccggcgcg gatatcgggg acctgtagcc aaccggatcg actcgcagag
     1921 gcattccctc gtcacatgga tgccgggggc cagctcccgt ccaggttttg ttcccgccat
     1981 ggcacatctc ggttccctca gtgcgtatag gttgagacgg cccctcccgt atcacctgcc
     2041 cagaaggacg tatgctgccg tgacgccgga cgctggcgtg tccgctccgc ctacccgact
     2101 cgatccgcac ccgtgcgtgc agatgagcgt gatcatgatc atcttgggca ttacccggtc
     2161 ctgggaggtg gatactttag agctgttttc agagaggtcg cctatctgta gcgaagggct
     2221 cgctgccgct tttcaccttg caggctcagc caggggggtc gctaccacac ggccccggcg
     2281 gcaaagccgc caagaagcgg cagcgggccc ttcttcgccc aagggctcac ccctgggact
     2341 tgcggtgcag gcggcccgtt cgcggccacg accaagtaat gtcctcctcc gagcagtaag
     2401 gacgacattg ctgcggcaat ggaccgctcc cgctcaattg cttcgcaatt gcctatgcgt
     2461 catccgctac actacgctct ccccttatta ggcgcacgca agcattagag caaggcaacg
     2521 cattcacacc ccaaccacgc aatgtggcat catccaccac gaacgtcatc gtctgttcga
     2581 ccgaccgcga gcgagagcct gtgagcaccg aaaccgtccg tatcgacccc gttacaaccc
     2641 gccccggcgg tcgtcgacgc tcccgcaccg aatcccttag ccaggctctc cgcttcccgg
     2701 caatcgtctg ggcgctgcac ttcctgctga cccagatccc cgcctccctt gcctaccgtt
     2761 acggcgagat ccgctccgtg catcgggtca atggagtctg gcaaccgacg gacggccagg
     2821 aatctggcgc ctaccaaatc attcgtccct cactcgatgg ctgggcacac tggatcgtcg
     2881 agccgttccg tagttgggac ggcacgtggt actcgatgat cgcggagcaa agctacagta
     2941 cgacttactc ggctctctcg gccttcttcc cgctctaccc gtggctgatg gaccttggca
     3001 gccaactgac cggtctgccc gtcgagaccg ttggctatct catctcccac cttgcgttcc
     3061 tgggcgccct gatcgtcgcc tacaaactgg tccgccttga cttcaacgag cgcgtcgccc
     3121 ggatgaccct cgttgccgtg acgatctttc cgacaacatt ctttttctcg gccatctaca
     3181 cggaatcgct ctttttcttc ctctctgtca cctgtctttg ggcagcccgc aagaacgact
     3241 ggctcctcgc cgggatcatg ggcttcttcg ccgcgatgac ccgttcggca ggtatcatgt
     3301 tgctggcgcc cctagccgtg ctcttcatcc agcagcacgg ctgggatctc cgccgctggt
     3361 tcccaaaagc actgcttggg ctccttctgc cggcgggcct tgccgtcttt ggctggttcc
     3421 tgacaacaaa ggggctcgac tttctcgact ggaagaacca gcagtggcaa tggaaccggt
     3481 tctcggcgac tccctggagg accttggagt gcacattcca gagctgtgag aacgtcacgg
     3541 tacggggttt cggtggcacc tatgagacga acgtgccgtc cgtgagcttc gcgtggatcg
     3601 gtgacctctg ggaccacctc agctggagct atttcacctc gtcggagttc cgctacaccg
     3661 tgggtcagag ccagtggctc gatgtcatcg tgacactcct ggcgttcgga ctgatactcc
     3721 tgggcctgaa gaaactgccg ctctactaca gcgcctggat gattccgccg atgatcgtgc
     3781 cgctcctggc gccgagcagc gtcttccccc tcatgagcat gccgcgcttc gtgctgccgc
     3841 tgctgccgct cttcgtaatg gcggtgttgc tgatcccgaa ccggaaggtc ggctatgtcc
     3901 tcgccggcac ctccgccacc ctgctcgtgc tccttaccac tcaattcgcg ctttggtact
     3961 gggtggcata gcggcatcag ggtgggcagt gcccgaaacc cagcccttgg caagcctgga
     4021 cttgtggcgg caactgcccc attttcctgg tctcccggta caatgcccgc agagtaatgt
     4081 gcagagaggc tggcaacatt gtgaaggtcc gaaaggtcgc cgaaggttcg tgtgctcttg
     4141 cgcagcacgg gcctgctggc ccggccactg ctgcccctca ggagcccagc gctggaatcg
     4201 tcctgcagat tactgacttg tccaacttga tggggctgga ggtgcctgcc ccgaacacgg
     4261 tacggagtac ataacgtggt taatgttcag gaatcacccg cgaaaccgtc gacggggtca
     4321 acatccatga ccggcggcca agcgctcatc tcttcgctgc ttcggcaagg catcgacacc
     4381 atcttcgcgc tccccggagt ccaactggac ggtgccttcg atgcgctcta tgacgcgcag
     4441 aacagcggcg atgtacgtgt catccacacc cggcacgagc agggggcggc gtacatggca
     4501 gacggctacg cccgcgcgac cggccgtatt ggcacgtgca tggtggttcc tggaccaggg
     4561 ctcctgaacg caaccgccgc actctccacc gcatatgcat gcaaccagcc agtgctctgc
     4621 gtcacgggcc aaatccagtc caacctgatc gagtttggcc gggggttgct ccacgagatt
     4681 ccaaatcagc tggggatgat ccgctccgtg accaagcatg ccgcacgagc cactacccca
     4741 tccgacattc ccggtcttgt cgacacagcg atcagggaac tctggactgg ccgtgtacgc
     4801 cctgtcgaaa ttgaggtgcc accagacacg cttctctcga cgggggacgt tgccttgctc
     4861 ggcgccgcac ccgcccgtca gcggacggag gcggacccgg agaagatcgc cgaggcggcg
     4921 aagctcctcg gctcctcgac gtccccgatt atcttcgtcg gtggaggcat ccatggctcc
     4981 caggcgtggg acgaactgcg tgagcttgcc gagatgctgc aggcgcctgt cgtgatgaca
     5041 cagaacggca agggcgcgtt gagcgatcgt cactacctgg cccagcacac attcgccggc
     5101 aagaatttgc tggccggagc cgatgtcgtc ctcgccgtcg ggacacggtt tgtggaaccc
     5161 accacctact catggcggct tcaggaagac cggacggtga tccagatgga tatcgatccg
     5221 gaggagatcg gccgcaacta ccccccgacg gtgggcgttg tctccgatgc gaaggttggt
     5281 ctggcggcac tgatcgagga gacggcccgg cacaaccgtt ctcgcccatc ccgggaggcg
     5341 gagttaagcg cgatcaagca ggcggcacag gagaagctgg acagcgtgga gccgcaggcg
     5401 ccctttgccc acgcgatccg acggcagctg ccagacgacg gtatctttgt cggtgagatg
     5461 acccaggtgg cctactggag caatgccggt ttcccggtct acgagccgcg cacatatctg
     5521 acgcccggct accaggggac acttggctgg gggttcccaa cctctctcgg tgtcaaggtc
     5581 ggggctccgg agaaggtagt ggtctcggtc aatggcgacg gcggcttcgg cttcgcccta
     5641 aacgagctgg cgacacaggc gcagcacaac atcaagagca tcaccctcgt gttcaacgat
     5701 ggtgccttcg gcaacgtccg ccggatccag acagaagact tccgcggacg caccatcgca
     5761 tccgagctca agaatcccaa ctatgtgaag ctggcggagg cgttcggtgt tgccggccgc
     5821 cgagcgacca caccagctga actcgagaca cagctcaaag aggcgattgc cgacgacgag
     5881 ccagccctga tcgagattcc ggtgcagccc atgccgaacc catggaagac acttgccctt
     5941 cgctaggggt cgcggacggg tgacgggtgg agacgcgaag ttctccaccc gttaccaccc
     6001 ggagtgctac tcatctccgt tcggcagcac tccctgatcg aggagctgcc gggtgatata
     6061 ggcgttgttc gccataatca gcttgattgc ataggaattc gcggcagcag cagcggcaaa
     6121 cccgggtgaa ttcagttcct cctccaacga attgcccgtc atggaggcag cttccaccgc
     6181 ctctccctgc agcagctcgt ccagaaaccc atacaattcc gcctctgaca ttcccaaaag
     6241 gccattttgg ccgtcgtttt ccatgttccg gctccttcgt caagcatcgg tttaacgcga
     6301 atacccttgc gcgacgcaag cgggaattcg atcaacatct cgattggcag cggactcgtg
     6361 cagtcgcaca atgcccggac tgcccttagc cgcggcgcac gaattgccgc tccctccaat
     6421 tgtgcgacac atcccggtcc tccctcgcct cagcgggtcg ggacttgcag cgcatcttga
     6481 ggtgcgtata ctcgagaata gcctgcgagt tcgtcgatga cgagcgccgt tcgcattaca
     6541 gcgggcccag tgcatgacct tcccatcgcc cctcggggca aggagtctgc gcatgacata
     6601 cgtcatcgct caaccgtgta tcggcaccaa ggacgcttca tgcgtcgagg tctgcccggt
     6661 cgattgtatt cactcggatg acgattccga gatctatcac atcaacccgg cggaatgcat
     6721 cgactgcggt gtctgtgccg aggtctgccc ggtagaagcc atattcttcg aagatgatct
     6781 cccggagcaa tgggcatcgt tcaagcctcg caacgccgcc tacttcgagt agggccgaga
     6841 cgacgctggg caaaagcggt gggcacgtgc ccaccgcttt tcgcgtattc cacatcaggc
     6901 tatgagcggc ttactgcggt actgggggcg ttgcaggggg cgtgcccgcc gggaccggaa
     6961 gcggcggcaa ggaataccgt gcggtccaca ccgcatcgcc attgacgaga tagagtgtgc
     7021 cggacgcctc atccaccgcg atatcctgga gatcatcgaa gggtgggagc acatcgcttt
     7081 cgcctgttga gaacccggcc ggtagctcca gctggctcat gtgccctcct tcacggtcca
     7141 cggcgatgac ccgcccatct ccctgatcgt cgacgaccgc aacgtagaga tagcccgtca
     7201 tcggcccact aacgattgcc agcggcgttt cggtttccgg atcaaacacg ggcaggtcga
     7261 acgaggccac ctgtgctccc cggcgcatcg tgagcacccg cccatcctcg agcaacacgt
     7321 agatattgcc gtccacggtc atgtctaccg ccagattcag cgtcacgcgg tcaccggtca
     7381 atacccaatc catcggccca acgcgttgct cctcgggatt aagcgtgaac tgatagatat
     7441 tccggtattc gggcacgagg agatagatat tctcggcaaa cgcggcaatc gggccgggcg
     7501 gccacggtcc ctgctggttg atctcctcaa gcgtcatgcc ctgccatgcg gaaccctcgg
     7561 cgctgccagc gaagaagacg tgctgtccgt cgctcgcgac caaggtttct ccatcgtagg
     7621 ccaccccgaa gagatccccg acctgcagcc cctccacatc gctcccggat tgaaggacga
     7681 gctgcatctc ccgagattcc ggccgcagtc ggtaaagact cccgtttgcc aggaagatac
     7741 ctcccggcgt gtggatggcc cgcgtccggg acgattgcag cgccgcgggc agccctccaa
     7801 tgcgcgtgac gtctgccagg cgaaccacct gttggatagc atcgcgttcc agggttatct
     7861 cattctgccg tggactgagc aactcctccg gggcgcctgc acgttccgca tccgccagtg
     7921 ccgcctccgc gtcgtcaagc gcgtcctgaa cgatgtcctc attcgacgta tcgcgggcta
     7981 gcagcaactg tcggtctgcc tccgccacat gggccatgta gtcgtcttcc gagggcagga
     8041 aacgaccacg ctccgtatac aggagcgccg acactcccag gagcgcgact acgcagagaa
     8101 cgatgaacac tggtgaccgg aggaacggca gcctcggcat cgtatgcttc cagctcggct
     8161 cgccagcccc gagagaactg tcacggtacc tgctgatgcc atgggcaccc ggtgccccta
     8221 gctgacgcag ctcggacggc tgacgccacg tgtctcgcca gtcaccggac cggcgctctg
     8281 tcaaccggat cagccgctcc tgaatgctct cctcgggctt ccgccatcca gcggccggta
     8341 cgcgtacaac gtcatccagg tcaccggcac cagaactcat atctatcgct gacactccgg
     8401 ccgccacggt gggcctggca gcgcgcgacg gacgcactgg gagaaattgc cggattgcgg
     8461 cgcgtccatg ccgccattgc tcgcgagcgt tgcggccaat atcgccgatc gtctcgacgc
     8521 ccgacccacg ttccgcgggc ggaaacccga tgacagccgc agcggctact gggagctcgc
     8581 gttcgatcac gacctcccga aggacgtcca tcaccttatc cggatcgcgc ccatgaaacc
     8641 cggagaccga cagctggtcg ccgcccggcc tagcgctcag ctccgccagc ccttcgccaa
     8701 tttttgccgt gcaaaggatg agggtgtccc cctcacggag ttccgtctgc accaacactg
     8761 gcgcagtcca tgaagcgtac ccaagcggct ccggggttgg cgcatcgtcc tgcgtttcag
     8821 cgtaatgagg cagccaggag ccgatctcgg gaaccgcgta gaccaggccg tcctgcgcca
     8881 gcacaagctg gccgggcggc aggtgcccga tcgttgcctg atggccctcg aagacgattg
     8941 ctgttgcgcc gatcaggtac cgctggccga tccccccggg aaagcggccc tcctcaaaaa
     9001 cgatgctgtt ggcgacggcg aacgcgcgtg caagcgcagc gtcggccggc tgccgctggt
     9061 gtcgtcgcaa ctcctgcagg atgagtgcac gcgcatgccg agccaattct tcgccgcgac
     9121 cagagagagt gagagactcg gtcatcaccg cgaacccgcg tcgatacggg gaaggttgtc
     9181 cgacgaagtc gaccgttatc cactctcgaa catcagcagg agtgccaccg gaaacggcaa
     9241 aggccactgc ggtgggcgcg gacaacgtca tctggaggga gcgcctgact caggggcggc
     9301 aggccgcgat gtacctgtat ccataaccgt tcgagtatat cggcctccct gaaccgcgtc
     9361 gaaccatcat ggaaaatcgg tcgagcccac atcagtccac gggccacgca cccaagttca
     9421 gcaagggtat taccggtcgc gactgtttag gaaacctggg cctggtcggg gccccggttg
     9481 aggtcttcgg cgatcggcgc caattgcggt gtccgcgtcc gagcttctcc catcggctgc
     9541 gccaccattg gccgtgttgc cacatcacgc tgaccggttc ggctcccgct tgagcgcgcc
     9601 tcccggacaa gcatcctggc gcgctccgca agcttttcac cgacgacccc gagactatcg
     9661 gccaagtgca gtccgtgttc gagcacctct acggcatcgc gccggcgtcc ttgcgcaagc
     9721 aggcgagcac ctacgttcgt cagcatcaat gtctcgccat actcatcacc cacctcacgg
     9781 ctaagccgga gggcgcgctc gtatgtatcg agcgcatccg ggtgattccc ggcatcctga
     9841 gccacggcca gatgctggag agcctgattg agcaacacag gctggtcgat cgtctccgcg
     9901 atctcaacgg cctgcgttag cgcatcgagg gccgcgtttg tgtcgccttg ccgctgggca
     9961 agccgggcaa gccgcccata gagccggaca gccgctgctc gctgaccgct ctgcgccgcg
    10021 acgtcaagag cttcgccata gagctggatt gcatctccaa cctggttcgc atccatcgcc
    10081 atcgtgccga gctgggagag caccgaaact tcctcgtcgc gtcgacccag ctggcgatac
    10141 aggtccagtg catcctggta gtactcgacc gactgccgga tcttgcgatc ggcgagatga
    10201 atctggccga gcaaggagag catgccggcc tgcaaatcga catcatcggc ccggcgcgcg
    10261 gcctcgatcg cctgccggat tgcctgtatc gcatcctcgc cctggtcgaa cttccagagt
    10321 gcctcgccga tactcccgag ccactgcgcc tcgtgagcaa cgtcctgggt tgagcgcgcc
    10381 agttccaggg cctgcatcag gctgttcaac gcgtcaaccg ggtgattcat cgccgataat
    10441 gtgacaccaa gcccgccgaa tgcccgctgc cggagacgag tatcaccagc atcctcagcg
    10501 agtttggctg catgctcgaa gacctcgcga gcatcctgca gccggccaag cgtcaacagt
    10561 gttgagccgt aatggatcgc atcggaggcc gctgctggca tattcccgag cctctcctca
    10621 acctggacga gatcgcgttg ccgatctact gctgtttcaa atcgaccgat cttttgctcg
    10681 acatcggaga gcattgccag gcaagcgttc tcaagctcaa gatcgccgac ctcacgagca
    10741 acctccagtc catcctccag atgcgcgacc gcgtcctcat atcgctccag cgccagcaag
    10801 gtgtgtgcgt gctggccaat gagttgggca agaatgacgt catcgcgaag atcggtggag
    10861 acgcgaagag cgtcttcctc catccgggcg cgagtctcga ggtcgagcga ttccccaccg
    10921 gtgtcgagcc gatggatcag tgattccgca atgccccgcg agtatcccag ccgctccgcg
    10981 gagcgaccgg cccgctcata gagatccatc gcaccttcgg ggtttccgtc ccgcaggata
    11041 atatcgccca gcaggatctc ggattccacc tcgtcttcgg tgtcggtgtt ctcgggaccg
    11101 agatccacct gctgttgcaa cagcacgcgt gcccgctcgg tatccccggc gcttgccgcc
    11161 cggttcccca ggcggccgag tgcccggcgt ccaccctgca cgtatcctgc gcgttgcgcc
    11221 gccttgtagg catcccgcag gagcttctca cgttcccggg cggggtcatc cgcggggagg
    11281 agcgtatcaa gacgcagcgc cgagtcgaca gtctgccggt cgaagctctt ggcgtgggca
    11341 gccgcatggg tgagacgcgc aagcacctgg gcgaactccc gatcggacgc aacgcgctcc
    11401 tcgatcaact gatccagtat ccgaaagtcg tcatcagaga gcgcgtcgag cacggcggga
    11461 tggcgaactt cgctgatcgc accctgaacc gagcgtcgac ggccaaccac ctcgcgcgct
    11521 atctgaacca ggacgccgtt gccaccccgt cggcccttcc cggccagttt ctccacccaa
    11581 ctgctcacca cggtcgctcc tcggccagtg ccttctccaa ggtctcaaga gtgagattcc
    11641 ctccagagag gacaactgcc actttcttcc cttgcaactc attacgtcgc tggtgcaatc
    11701 cggccaaccc agccgctccg gctccttccg caaccatgcg ggttgtttcc aataatgtca
    11761 ccatggacct gcggaggtca gagtcggaaa caaggacaat gtcgtccact tgattccaga
    11821 gaattttcgc cgggaggctg aacgcctcgc ttgtggcgat gccctccgca aaggtcttcg
    11881 ccggaccaat cttcttcaat gtcctctctc gccaactctc gtagaccacc ggggcgccct
    11941 cggcttgcac gccatagact ttcagccggt cagagatacc cttgccagcg atgcaggcgc
    12001 cgcacgctcc actgccaccg ccgatcgcga cgaatattgc atccaggtcc gggtgttcct
    12061 ccatcagttc cagcgtcgag gttcccactc ccgcgatcaa ctgcggcacg ttcgccgagt
    12121 ggatgaagta cttcccttcc gccttcgccc agcgtccagc ctcgtcgtgg caatcattga
    12181 attcatcgca ggcgaagcgg acatccgctc cgagccgttc catcgccgcc accttgagcg
    12241 ggttcgcccc attcggcacg aaaatagtgg cgtgttcgcc gaagattcgc gcagcgtatg
    12301 cgatcgactg tccgtgattc cctgtcgacg ccgccacgac gcctgcggct cgctcgctat
    12361 cggagagggt actgagcaga tagatgccgc cgcgaatctt gaatgcaccg ataggttgca
    12421 gattctcgca ttttaccacc acccgacagc ccatccgttc agacaacgcc ggcgattcga
    12481 tcgccggcgt tggttgcagg tactgacgaa caaccttcct cgcctggaag acgtccgcca
    12541 gagttgggac tggcccggac cacacctctt gctgctccac gtgctgcact ccaacctcgt
    12601 cgctacagcg gcaaatatgc cggaaatttc tgcgtcatcc cggaaacggt atgcagtgca
    12661 cgtacaccct gtacatattg gctggagtgt accacgggca caatacaggt gaaacctgtt
    12721 ccagattgtc acaagcactg gacccgttcc tttccggttg ttatctggta tcgtaaacag
    12781 cgtagttgcc ttattcgcgg cttctcgcat ctggtttgaa cgccatgcct acttcccttt
    12841 catttcagga caagctcgat gcccggatcg gcgccgcgga ttcactcctt tgcgtcgggc
    12901 tcgatcccgt cacagcgagg atgcctcagg gtttcgatgg ctccccggaa gcagttgttt
    12961 ccttctgtaa ggaaatcgtc agcgcgacca ctgaccttgc cgcggcatac aaacccaacc
    13021 tcgccttctt tatggccctt ggcacgcgag ggctggaagc actccattcc gttcgagagg
    13081 caataccgtc cgacatcccg gtcattctcg acggcaaact gaatgatatg gggcagacag
    13141 cgaaggccta cagcaacgcg gtcttcgata cccttggctt cgatgccctg accgttgcgc
    13201 catacctggg cgaggacgct ctggcgccat acctggagaa gggaggcagg ggcgtcatcg
    13261 tgctatgcaa gacgagcaat cccggtagcg gagatctcca ggacctggaa ctcgcctccg
    13321 gtgaactcct tcaccttcac gttgccgacc gctgccaaga atgggaccgt cgctatcccg
    13381 cgaacgtcgg catggtggtg ggagcgacct accctccaca acttgccgag gtccgccgac
    13441 gttgtccgga ccaggtgatc ctgctacccg gtctcggagc ccagggagga gatgcagcct
    13501 catccgttcg cgctggtatc agcagttccg ggggccgctt gctctgttca gcatcccgct
    13561 cgatcatgta tgctggagac actgccgact tcgccgaagc agcccgggct gcagccggtc
    13621 ggctccgcga tgagatcaat gcccaccgca gggctcgacg atctgcctga cgcggcataa
    13681 ctccacattg atgccccgcg cgtatgtctg tttggtggcc cgtcacaggg ggcgcttgtg
    13741 ttcgctgtgt tcgtttcgat aggtgtctgg cgcgaccgtc acccggctct acactatatt
    13801 cctggcagga gagcaatgcg caccgtactt cattcgctcg tcacacgtgt ttttttcacc
    13861 atactggcac tgaccctcat cgctcctgtt ctgccgggaa tggccccaca acctgccagc
    13921 gccaactccc tggcagaaca acttggcatt gaccccgagt acattccgcc agacttcggc
    13981 cagaccgcgc ttagtgtcta cgtctctgcc acaggtcatt ccgtccaggg ctacatgctc
    14041 gattactggc gtgccaacgg cgcagcctcc gtctacggga atccaatctc agaggccttc
    14101 ggcgcccgca acggactcta cagccaggcc ttcgagcgtg ggatcttcca gttctctcct
    14161 gaatgggtgt ggaccgatga tgctgccgtg cgcctgatgc cgatcggcac ccaggaggta
    14221 cgggaggacc gtctggcgac ccgctccgat ggccgtcgca ccggtgctga tcgccgcgcc
    14281 aacgcctggc gcccggggtc ggagtccagc gtccgcgcca gtgaggtctc caacgagggt
    14341 ggtcgcttca gcaatctcac cggctacagc atctccggtg cattcgctgc ctggtatgac
    14401 agccacgagg gctggttcta tatgggtgca ccgatcagcg agccccatat cgagcgtggc
    14461 gtgcccgtgc agtacttcga gaacggcatc ctgatgcagc aggaaggcgt aatcaccccg
    14521 gcgcccctgc cacgcgagaa accggaagcc tacggcattg ataccacacc ggtggcgcag
    14581 gacgggcgac cggagttctc cgaggcgctt ttcatcaaca accccaatcc gttcggcgtc
    14641 gacgttacgc agcttagtgg gcgcaagcgt atcgtcgtca acatcccaga gcagaagatg
    14701 tgggtttatc agggtgacac gctcgtgctc gagacctttg tcagcactgg ccttagcccg
    14761 aatgacacgg aagcaggcaa cttccacgtc cgtatcaagt tcgagacgca ggatatgtcg
    14821 ggctttacca gcgatacggg tgaggttgtc ggactcggcg acgaggaatc ggccgagggc
    14881 acggtgactg cgaacaccta tgaggtagcc gatgtacccc acgtcatgta catcaactac
    14941 gaggcggagg cgttgcatgg cgcatactgg cacaacaatt tcggccagaa aatgagccac
    15001 ggctgtatca accagccgct cgacgttgct gcattcctct atgactttgc tccgctggga
    15061 acggcggtag acgtcattga gtaggacaag cccgacgata tggcaacgcc ggtcgggaag
    15121 agccggtcgg gcgttgccat atacaggtga tcgatggcac gaatccttta tgcttgtacg
    15181 aggtttccgg catgggccac caccggaacg agccgcatgg actcccgcat tgggattacg
    15241 ccaccgggac accattgaac gattcaccaa cgggctctag gcgcatagcg gagaaagctc
    15301 ccccctcatt ccctgtccag atcgtcgcgt tcgccaacca gaagggcggc gtgggaaaaa
    15361 cgaccagcgc gatcaacacc tccgtggtgc tggcctcgcg cggctaccgg gtattgctgc
    15421 tggacatgga cccgcagggg aacgccacct cgtcgctcgg tatcgacaag caggagcttg
    15481 atgcttcgat ctacgacgtt cttgtcgagg acgcttcgat tggcgagtgc cggatcagcg
    15541 atgtccgccc cgggcttgat gtgctcccga cgacctccac cctcgctgcc gccgagattg
    15601 aacttgtcgg cgtatccgat cgggagcggc ggctcgccag ggcgtgtgcc ccgattcgac
    15661 atgagtacga cattgccctg atcgactgcc cgccctcgct cggtctcttg accattaatt
    15721 cgctgacggc tgcgagctcc gttgtagttc cgatccagtg cgagtttctc gcccttgaag
    15781 gcgtcagcca acttattcac acgatcgatc tcacccgccg ccagctgaat ccgactttgg
    15841 atctcctcgg cgtgctgatg accatgttcg atgcccgcac gcggctgtca gcccatgtgg
    15901 tcgatgaggt gcgccggttc tttccggagc ggatcttcga gaccatcgtt ccccgctccg
    15961 tccgcctcgc cgaggcgccg agctacggcc aggccatctc ggaatacgac cctgtgtcac
    16021 gtggtggttc cgcatacgca tccttcgcga acgaactcgt ggctcggctg ggcctcaagc
    16081 agccaagtgg aacagacgtc aactcgtcac gctgatacgc aggagaacac cctcacatga
    16141 ccgaccctca accgacttct tcgcccatgc gtgaggataa attcgccggc cttggcctca
    16201 ccttcgatga tgtcctgctg attccggcca ggtccgatgt catccccgcc gatgtctcaa
    16261 cccggacacg tatcgccgcc gatatcgaac tcaatatccc aattgtctcg gcggcgatgg
    16321 acaccgtcac ggaagctcgg ctcgctatcg ccatcgcgcg tgagggtggc attggcatca
    16381 tccaccgcaa tctctcgatc gcggagcagg cggatgaagt ggacaaggtc aagcgctcgg
    16441 aatccggcat gatcgtggag ccggtgacgc ttggaccgga tcagccggtc aacgacgcgc
    16501 tctcggtgat ggagcggtat cacatctccg gcgtgccaat caccgaagat gacggcaaac
    16561 tggtcggtat ccttaccaac cgtgacctgc gtttcatcca gaacccggac cagatgatct
    16621 ccgaggtaat gacccgggag aacctgatca cggctcccgt gggcaccacg cttgaggaag
    16681 cggaagagat cctccatcat catcgcgtcg agaagctgcc ggtcgtagac gataatgggt
    16741 gcctgaaggg actgatcacc gtcaaggaca tccagaagaa gattcagtac ccgaatgcca
    16801 ccaaggatag ccagggccga ctccgtgtcg gcgcggccat cggtgtcggt cctggttctg
    16861 aggagcgggc tcaggagctc atcaggagag gggttgatct tctggtcgtc gataccgcgc
    16921 atggacacaa cagtggcgtc ctcaacatgg ttcgcaggct caagagcaag cacagcatcg
    16981 ccatcgttgc aggcaacatt gctaccggtg ctgctgccga agcactgatt gacgctggag
    17041 cagatgggct caaggtcggt atcggcccgg gctccatctg cacgacgcga gtcgtggccg
    17101 gcacaggtgt gccccagatc accgccattc acgaagccgc caaggcagcc cagaagcaca
    17161 acattccgat tattggcgat ggcgggatcc agacatcggg cgatatcgcc aaggcgatcg
    17221 cggccggtgc agacgcagtg atgctcggca gcctcctcgc cgggctcgac gagtgccctg
    17281 gcgaggtcat cgtcttcaag ggagagcgct tcaaggaata ccgcggcatg ggttcactcg
    17341 gcgcgatgcg tggccgctcc ttctccaagg atcgttactt tcagggcgac atcgggaatg
    17401 tctccaagct cgttccggag ggcatcgagg gccgagtggc ctacaagggc cagcttggtc
    17461 cgcttgtcta tcagcttgtc ggcggactgc ggtcggccat gggatatgca ggcgcgcctg
    17521 acatcgccac gctgcggaac gactcgcaat tgatccagat caccaatgcc ggtctggccg
    17581 aaagccaccc gcaccatgtg actgtgacgc gggaagcacc gaactaccgg atgagcagct
    17641 aggacgcatt ctcagcacac cggtccccgg agtctcctga tgcgaaatct cgtgtatttc
    17701 gtagcgacga caattgacgg gtacatcacg accgccagcg atgttgaccc tgatttcctg
    17761 ctgtatgacg gcccgcaggc cgcgaatctt ttgcaggagt tcccggagac catccccgga
    17821 caccttcggt cacttctcgg tgtaccggat gaaacaccaa accagcggtt tgatacagtc
    17881 ctgatgggcc gggccacgta cgatattggt cgtgcaatcg gagttaccag tccctacccc
    17941 catctacgtc agatcgttgt gtcttcttcg cttggcccct cacccgaccc agcggtcgag
    18001 gttatctcgt ccgatgtcgt gagccaggtc catgaaatca agcggagcaa tggcaaggac
    18061 atctggctct gtggcggcgg taacctcgcg gcgtcgatta tcaacgagat cgatgagttc
    18121 atcttcaaga tcagcccggt ggtgcttgga ggcggggtct ccgtcttcgg tgagctcgtt
    18181 ggaccacgga agctcaccat gacaagccac cgtgtgtacg agaacggctt ctccctgctg
    18241 cggtaccggc aagttgcagg ctcatctacc aatgccctcc acccaaaaac ttctcactcc
    18301 gccaggtgac gagccgcctc gcgtaccgac tggaccccat cttgtcaggc gcgtcccata
    18361 ccttgcggga cagttcctgc tctatttggt gatgttccag atctacaaga tggtccggat
    18421 gcagttcatt ccccacgacg aatctgtggc gtacaggcat gcgaaggaca tcctccacct
    18481 tgaagagcag cttgggctca tgttcgaact cgatctgcag cgatgggtgc tcgaccaagg
    18541 cgattggctc atcacctttt tcaacaacgt ctatgcctac tacatgtggg gcttccagtt
    18601 cggcatgctc ttcctggcct ttatggccct tcctcgctac cgctacatgc gccgggcgtt
    18661 cttcatcagc atggcgatcg ccacacccat gtacctgatc tacccgctgg cgcctccccg
    18721 ctttatggag ccgtacgggt ggcccttcat ggatacgctg gccgtctatg gccgaactac
    18781 tttttcgaga ccggcctcgt gaccgcaaat cgttttgcgg ccatgccgtc gatgcatgtc
    18841 ggctggacca ccttctttgc catctgcatc atgttgggga tcccgaaccg gaagctcggg
    18901 atcgctgtca tggtgg
//
LOCUS       DASYWM010000272         3858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_594986, whole genome shotgun sequence.
ACCESSION   DASYWM010000272 DASYWM010000000
VERSION     DASYWM010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594986"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(405..995)
                     /locus_tag="VGR29_11140"
     CDS             complement(405..995)
                     /locus_tag="VGR29_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074182.1"
                     /translation="MMKRTFVPTTFVAAIIFAMSIVFGAQVTSGQGGAATPSTQLDTE
                     GGAQAHPAHIHSGTCEELGDVVFPLNDVAPLEADATPAASPAASPAAGLPGEMVVAES
                     TTILEVALDDILGGEHAINVHESAENIDVYIACGNITGTPENNHLRVDLQELNDSGLS
                     GRAVLTANPDGTTTVTVTLTQAEDGATGTPEASPAT"
     gene            1261..1560
                     /locus_tag="VGR29_11145"
     CDS             1261..1560
                     /locus_tag="VGR29_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074183.1"
                     /translation="MTTEDDVQLISEFEARHPELTVALLVPDSPPGDSVTVRMFDKDR
                     PSTPKWEGQGQTVREALEDVESQLHSAQGEQPLQEEKEFLEDVYREIHDGSNKLD"
     gene            complement(2766..3743)
                     /locus_tag="VGR29_11150"
     CDS             complement(2766..3743)
                     /locus_tag="VGR29_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074184.1"
                     /translation="MRRLFFGFLAAILLLPMSMVSVLAAQDATPESAFSDLGLPTLDI
                     TATADAYEGIPDSIEAGRYLVTVTALEEAGQFGAGVAFIQPAGVTGDEFISMLGEVSG
                     PPDESGVGAAAATPIEGGAATPAEGTEEMGGPPDVFYQSLYAGGTFALPGETSQVVLD
                     LTPGEWVAWGDDPGSPQAPVAFEVTGEMPAELPEPESAATLTMGEYVIEVTEGEITSG
                     QQVLRIDNIGAQPHFIAAGTTQLDVTGADIEAVLEAEMTGTPAAVDFNPDEDLEEAFY
                     SGTQSMGTSQWIVADIQPGTLIMICFFPDIEDGMPHAFYGMYTIVEVAE"
BASE COUNT          819 a         1141 c         1130 g          768 t
ORIGIN      
        1 agcatggaga gcgaaataat cgccagaaga gagatgatcg cgaataccat acggtaaacc
       61 tcctgccacg aagatcatgt ctctgtaggt ttgaatgtag tcgtcggcag taagaatgac
      121 ccatcttacc agtcttacac caatgtgttg ttcggtgctt ggaattgatg gggcggagtg
      181 tttccgtata ttgaaggcag gcacttaccg accgcaaagt tcgagctcgg attaccatgg
      241 ggatcggtcc ggacgcccat gtatctacga tggtggcccc ttgttcgaat cgtggccctt
      301 gaaacccggt ggtggagaga ggcctggaag gtgcacacaa acggaccacg gaaagagcat
      361 aagccctcac cgtggtccgc cggaacgctt cgtctgatcg tgctctaggt ggccggagat
      421 gcctcgggcg taccggtggc cccgtcttca gcctgcgtca acgtaacggt cacggtcgtc
      481 gtcccgtccg ggttcgccgt cagcacagcg cggccgctga ggcccgagtc gttgagctcc
      541 tgaagatcaa cccgaaggtg attattctcc ggcgtcccgg tgatattgcc gcatgcgatg
      601 tagacatcaa tattttcggc gctttcgtgg acattgatcg catgctcgcc accgaggatg
      661 tcgtcaagtg cgacctccag gatcgttgtg ctctcggcaa ccaccatctc gccaggcaag
      721 ccggcggcgg gagaagcagc cggtgaggcg gccggggtag catccgcttc aagcggcgca
      781 acatcgttca gcgggaagac aacatcgcca agttcctcgc aggtacccga gtggatatgc
      841 gctgggtggg cttgggcccc accctcagta tctaactgcg tagatggggt agcagcaccc
      901 ccctgcccgc tggtgacttg ggctccaaac acgatgctca tcgcgaaaat gattgccgca
      961 acaaacgtcg tcggcacaaa ggtgcgtttc atcattcgat attctcctgt gtaacgtcgt
     1021 gcgttccggt ggtaccggat ggtttggtcc ggacttcctg acgcgcatgc tttgtgccac
     1081 gctggtacgc cgattcgcga tcgaccacat cgatgcaagg tttattcacc acgcaattgc
     1141 actgattgca gggatcttct tccagagacc accgtttgtg aacataggtg aacaggaatt
     1201 gcgtgggtgc agaatcagtc cgtatcccgc gggctcccga ctagatggaa agaggtaact
     1261 gtgaccaccg aagatgatgt ccaattgatc tcggaattcg aggcccggca cccggagctt
     1321 accgtcgcgc tcctcgtgcc cgattcgcct cccggagata gcgtgaccgt ccggatgttc
     1381 gacaaggacc gcccgagtac cccgaaatgg gaggggcagg gccaaacagt tcgcgaagct
     1441 cttgaggatg tcgagagcca gctgcattcc gcccaggggg agcaaccgct tcaagaggaa
     1501 aaggaatttc ttgaagatgt ctacagggag atccacgacg gcagcaataa actggactga
     1561 tagtcggcta cgttgctgtt gccggaagct tgccccggct ccatggagta cctctctgca
     1621 tggcgacacg ttggctgcaa tcctaggaat ggagcatcgt ggacactcgt agagcagtgt
     1681 gacgatgtcc gtgacgaaag tatgcgagct tcttttggag tccggccccc agatcccgcc
     1741 caaacggcga cctcattggc tcattctatc tattaaaaga gaggacgccg gacgaagggg
     1801 gtgtccggcg tcttcctgtg ggtggaggaa gggggaattg gcgtccctgt cgggctccgg
     1861 gacaggagcc tctcctcgac aggacacaga ccccgcgcat caggatccag caccacacag
     1921 gatcgattcg atgtcacgtc cagatatctg gaagaggctc cgttggcacc acggtggctg
     1981 gatccgtgtt cggtgccaag tcgcctcccc gtcttccgga agggtcccct atagtctgcc
     2041 cggttcccct ccccggcaca tccgcaaaaa catgtatttc catctccgac taaagaccta
     2101 gatccggccc gaccgcgatt aaattcaggc aagaaccacg gcgatgacac cctgtgagat
     2161 cacacagtcg accaaggcaa gtttgctctg gtggatcagc gtcggtacga atgtggcgcc
     2221 ttggagagtg aaacagcgcg cagccgtgct gcatctggag tcaggaggtt ctcgatggtg
     2281 ggcatcctga tcgagcagga gtggccgcag ggcgacgtgc acggtaatca acatcagaaa
     2341 aggaaccctg ccacagttgc tgtcaggggg acaaaacatg gcaggacctc ctcgacggta
     2401 taacgcagct ctactactgt ctggatcact tgcaggaagc ggtaccacag catccgaagg
     2461 ctctggcgtg agcgaacccg tcccaacatc cgattcggac ggttactatc tcagatctgg
     2521 ctcttgcttc ggctaacctg aaccagggca acatcaggcg cacgacaacg gcccctgaca
     2581 gctaccatga gccacgcctg ctacgtgtat tgggtgcggt ggtcgaaacg caacttgcct
     2641 atgctgcggc agatccgacg cccatcgact tcatcagatc gaacaggatg tgtgcactcg
     2701 ttctccaggg cagtgcagcc gggcgcccag ctgcactgcc ctggtgacat gattgtcagg
     2761 cgatgctatt cagcaacttc cacgatggtg tacatgccat agaaggcgtg cggcatgccg
     2821 tcctcgatat cggggaagaa gcagatcatg atcagagtcc cgggctggat atccgccacg
     2881 atccactggc ttgttcccat cgactgggtc ccactgtaga acgcctcttc aaggtcctca
     2941 tctggattga agtccacggc ggccggtgtt ccggtcatct ctgcctcaag gacggcctca
     3001 atgtcggctc ccgtcacgtc cagctgcgtc gtaccagcgg cgatgaagtg tggctgggcg
     3061 ccgatgttgt cgatgcgcag cacctgctgc ccgctcgtta tctcgccctc ggtaacctcg
     3121 atgacgtatt cacccatcgt cagtgtcgcg gccgactcgg gctccggtaa ctcggccggc
     3181 atttcccccg tgacctcaaa ggcgaccggt gcctggggtg atccgggatc gtctccccac
     3241 gccacccatt cgccaggcgt caggtcaagg acgacctgag aagtctcgcc aggaagagca
     3301 aaggtgccac cggcgtacag tgactggtag aagacgtcag gcggtccccc catctcctcg
     3361 gtcccctcag ccggcgtggc ggcaccgcct tcgatcggcg ttgcggcagc ggcaccaacg
     3421 ccactctcgt ctggaggtcc ggaaacttct cccagcatgc tgatgaactc gtcaccagtc
     3481 accccggcag gctgaatgaa ggccacgccg gcaccgaact gtccggcttc ctccaatgcg
     3541 gtgaccgtga ccagataccg accggcctcg atggagtccg ggatcccttc gtaggcgtcg
     3601 gcggtggcgg tgatatcgag cgttggcaat cccagatcgg aaaaggcgct ttcaggcgtc
     3661 gcatcctgtg cggccaggac agacaccata ctcatgggca acagcagtat ggcggcaagg
     3721 aatccgaaga agagacggcg catgggtttc tcccctgtaa tggcgatacg gccaaaaatc
     3781 gaagggacga tacgaacacg catgtgtcaa ccggcgccgg cctcacatcc gcaccgtacc
     3841 gtcccgtcaa cacaatac
//
LOCUS       DASYWM010000273         8261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_388186, whole genome shotgun sequence.
ACCESSION   DASYWM010000273 DASYWM010000000
VERSION     DASYWM010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388186"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VGR29_11155"
     CDS             <1..1173
                     /locus_tag="VGR29_11155"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEV2074185.1"
                     /translation="HYISLLSSLQEGGFNELMRLLVETLRTHRATLLIIDGAGAARML
                     AESDFDYARFMHGLQARTALLNCTMVLLAGEREADVAATHVDGLIQLSNRPTHARDTR
                     WLRVAKLRGSNYLNGQHRFAIGSNGITVFPRLESTQAQMAPAWFDPQKRLPVGIPGLD
                     TALGGGLQEGSSTLAVGTTGAGKTLLGLHFLAEGARRGEPGLIASFNETPRAVVSTAD
                     SAGMGLSPHVDSGLVQIMWHPPLELAPDEWAWQLLATIDEHQPRRLVVDAISDLLPLF
                     AIPERKSLFPPALVNRLRDRGVTTVFIVEVDAFVSPSLIVPVENLSATMDNGILLRTV
                     EVRSSLRRMISILKQRQGAFDPTIRELLVGSNGLTVGDPFDASGLMTGSSAPFSQR"
     gene            1203..1580
                     /locus_tag="VGR29_11160"
     CDS             1203..1580
                     /locus_tag="VGR29_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2074186.1"
                     /translation="MKRILAVDDEPDILELLVEILGIEGYAISAASDGRAALELLQGT
                     GADLVITDTMMPRVDGVALIRSMRDSPGLCDIPVIIMSAAGRPNLDGLDICAFLHKPF
                     DLIALLDAVAEAIDCGPSQKPSA"
     gene            1879..2862
                     /locus_tag="VGR29_11165"
     CDS             1879..2862
                     /locus_tag="VGR29_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2074187.1"
                     /translation="MDIDSWSDADLVRAARAGDSFSFGVLLERYRPPLHALALHMLGH
                     GPQAQDAVHDTFLIALHRLHTLQEPGAVGAWLRAVTRNVCLETLRKTPVVMPLDDLSF
                     GRTLRSPEPSLEERIDQLALKDWVWTALSELPENLRVTAMLRYFGTRASYEEIAAVLG
                     VPTGTVKSRLNQAKAKLAEALLATANLDHSGARQLNESATGYFLAATDQMNRGQGYNI
                     FADAFSDQPEVMLPDGSKLRGRHYLIESLEEDMDAGIKMHLSHVYASKTVTVLEATFE
                     NPASNPTHCPPATTQVHFKRDGKADRIRLYFAPRPATEQGKGPVSEEVNQS"
     gene            2902..3285
                     /locus_tag="VGR29_11170"
     CDS             2902..3285
                     /locus_tag="VGR29_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074188.1"
                     /translation="MMHGKQESIPVVFEQDGVVSRQAQWGEMTVAFERAPVGTDTRPL
                     FQGLPGDSCQCPHWGYVISGQMRVIYPDREEIVGTGEAYYLAPGHNIVCDAKGEVVEF
                     SPHGQYQKTMQAVATRLATNEDKDS"
     gene            3774..6431
                     /locus_tag="VGR29_11175"
     CDS             3774..6431
                     /locus_tag="VGR29_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV2074189.1"
                     /translation="MPVDATPHLQLASELAGMLTWELDLDKGTVRWSPDAERVLGRKP
                     PASLASILACVHPDDREVARQQFNDLARGNNRIDITARIPDPARQRDRWFHCRGARFH
                     DDATGCDRILGIAQDVTGYKQRELNVSFLAEMREHLAAAGTEDVLQVFGERTLTHLSL
                     SRLTLSALSDEKDRLLVLHDQHLTGNPCEKIPGLTGSLLPDMKGALLGGQPVAIEDVD
                     RDPLTAHMADEFHGCGVGSLLYVPYPATGQWHMLLGACKDSPYTWDTGEIVLLQDLAE
                     RLSLRFEHVRIVQELRQSEEQYRALFDSIDEGFCIIEILLDEAGVPTDYRFLETNPMF
                     EQHTGLHNALGKTARELVPDLDEHWFEIYGKVALTGEPVRFVQHAAAMQQRWFDVNAF
                     RIGDPGQRRVAILFKDISEQKRIEQTLRQSEERLRRAIEIETVGIIFFHPQGQVIDAN
                     EAFLRMSGYTRDDLEEGLLRLDTLTPPEWMPESRRAIDEFLSHGRIAPYEKEYLRKDG
                     SRWWGLFAAARVGDNEGVKFIIDITASRQAEAERARLADIVDSSRDAIILCDPDGTII
                     DWNGAAEELYGYTSDEVIGQHIRLVVPPDRRHESRGAFERLVDGEQVPPWESVRTRKD
                     GSTVYVEIRLSPIFGPQGKVVGASAIVRDISERRRLAQAQDDFLAMASHDLRSPVTVL
                     LGRAQLMRRRKTYDEASIETIITQARRIERLAMDLQQVVQMESGQLQLNCSPVSLGTL
                     AREAVDRVRSITDAFDFRVDVPEDPVTGNWDAHRLNQILDNLLGNAVKYSPDGGRIRI
                     AVSENGESARIQVTDQGVGIPDSMQKMLFTRFYRADEAGVASGLGLGLYISRMLAEAH
                     GGSISVDSAPGAGSTFIVDLPFAMPGRQA"
     gene            6604..8070
                     /locus_tag="VGR29_11180"
     CDS             6604..8070
                     /locus_tag="VGR29_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEV2074190.1"
                     /translation="MPVEITALPGVTTASGDIPVSSRRVEAGATRLRLVHPLISTALF
                     FLACWHFFGAIDDRPMHRDEARWIHRAAYIRELMHPFSSYWDEETWIAKGGTLDERYR
                     LRAQPPMGSYVMGIGFLLQGQDLPDIGFWNMDQDDAWNIKRGNQPSPSDVTTARRTSA
                     IVGALTVVSVYFIASRLTNPVGSLMAGIFLAFHPLMSHLATFAGSDAALGLSIAAAAI
                     AAYRLADRPSWLRAILLGVILGLGASTKLSPLGVTAPLALLGVIAVAYRLLLLVRETR
                     GNKPPSAESGRNTWPSWRLGLGLLSTPVVALFTFIASYPYLWQDPVANIQALVDYRQW
                     GMDIQGMLWEHIAVETRTEALQRVGARLGTEWTTLGRLVERFGWSWIPAGFELTLAAA
                     GLVLLLWLVAQRGVWSGPALTATVLTGQAAITIYGLGVDWARYHLPVLLLVAVCIGVL
                     AGEIMAGLGRLPSFRHPSPGIPHELPPPPDSPADLDGR"
     gene            complement(8026..>8261)
                     /locus_tag="VGR29_11185"
     CDS             complement(8026..>8261)
                     /locus_tag="VGR29_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074191.1"
                     /translation="REHLADTPSPSTTGNLYAPMPALNDVRGGWKDGSPKGTRAAKVG
                     MAVVPLALLWRRWSDSDRSSAAVKIRGGIRWRR"
BASE COUNT         1588 a         2634 c         2421 g         1618 t
ORIGIN      
        1 cactacatca gcctcctcag ctcactgcag gagggaggct tcaatgagct gatgcgcttg
       61 ctcgtcgaga ccctgcgcac ccaccgggcg accctcctta tcatcgacgg cgccggagcc
      121 gcccgcatgc tcgccgagtc cgatttcgac tacgcccgtt tcatgcacgg cctgcaggcg
      181 cggaccgccc tcctcaactg caccatggtg ctgctcgccg gtgagcggga ggcagatgtc
      241 gccgccaccc acgttgacgg cctgatccag ctctccaaca ggcccactca tgcccgtgac
      301 acccgctggc tgcgagtagc taagctccgg ggctccaatt acctcaacgg tcagcaccgg
      361 tttgcgattg gcagcaacgg aatcactgta ttcccacgtc tggaatcaac gcaggcgcag
      421 atggcaccag cctggttcga cccacagaag cgcctccccg tcggtatccc gggactcgac
      481 acggcgttgg ggggcggact tcaggaagga agttctaccc tggctgtcgg caccactggt
      541 gcaggcaaga cgctgctcgg cctgcacttc cttgccgaag gtgcgcgccg cggtgagcca
      601 ggtctcatag ccagtttcaa cgaaacccca cgcgccgttg tctctactgc cgatagcgcc
      661 ggaatggggc tcagcccgca cgttgactcg gggttggtcc agattatgtg gcacccaccc
      721 ctggaattgg cgcccgacga atgggcctgg cagctcctgg cgacaataga cgagcatcag
      781 cccaggcggc tcgtcgtcga tgccatcagt gacctccttc cactctttgc aatccccgag
      841 cggaaatccc tgtttccccc ggcgctcgtc aacaggctcc gggaccgggg tgtgacaacc
      901 gtattcatcg tggaggttga tgccttcgtc agcccgtcac tgatcgtccc tgtggaaaac
      961 ctctcagcga cgatggataa cggcattctc ctgcgtacgg tggaggtgcg atcctcgttg
     1021 cgccgaatga tatctatcct caagcagcgc cagggagcct ttgatcccac catccgggag
     1081 ctgttagttg gttcgaacgg cttaacagtg ggcgatccat tcgatgcaag tgggctaatg
     1141 accgggagct ccgcgccctt ctctcaaagg tgaggcggca tagctcgtgc gtaatggcga
     1201 ggatgaaaag gatcctcgcc gtcgacgacg aacccgacat tctggaactg ctggttgaga
     1261 tccttggtat agaaggatat gcaatctccg ctgccagcga tggcagggca gcactggagc
     1321 tgctgcaggg aacaggcgca gacctggtga tcacagacac gatgatgcct cgggtcgacg
     1381 gtgtggccct gatccggtcg atgcgcgata gccccgggct gtgcgatatc ccggtcatca
     1441 tcatgagcgc ggctggccgt cccaatctgg acggtctcga tatctgcgcc ttcctgcaca
     1501 agccattcga tctcatcgcc ctcctcgacg cggtcgccga ggccatcgac tgtggacctt
     1561 cgcagaagcc cagtgcctga gaatcccccc tctctcccct gcatctcgtt aggctgtgat
     1621 cccccgccag gtccctgctc ggtgaacttt ggaaaccgcg ccggtcagat ttgatccctc
     1681 ggacacccat cgcctgcgct ccccttggct gagcggccct ttcggtccct cgctgccgtc
     1741 cagcggggtc ccacgggcac actaggcgac atcaggaaaa aatctcctcg gaacaatcga
     1801 acatttgctt gtgctcgtgc atccttgtta cgtagcgcag aacggttgca acgcactgag
     1861 ccggaggcag gatcgatcgt ggacatagat tcctggagtg atgctgatct ggtgcgagcg
     1921 gcgcgagcgg gtgactcctt cagcttcggt gtcctgctcg aacgatatcg cccacccctt
     1981 catgcgttgg cgcttcacat gctcggccat ggaccccagg cgcaggatgc cgtgcatgac
     2041 accttcctta tcgcactcca tcgattgcat accttgcaag agccaggggc ggtcggggcg
     2101 tggctacgcg cggtaacccg taatgtctgt ctggaaacat tgaggaaaac gccggtcgtc
     2161 atgccgctgg acgatctctc tttcggccga accctgcgat cccctgaacc atcacttgaa
     2221 gagcgcatcg atcagctcgc gctcaaggac tgggtatgga cggctctctc cgagctgcct
     2281 gagaacctgc gagtcaccgc catgctgcgg tacttcggga ctcgcgcctc gtacgaggag
     2341 atcgccgctg tgcttggcgt gccgaccggc accgtaaaga gccgtctcaa tcaggccaag
     2401 gccaagctgg cggaagcgct cctggccacg gcaaacctcg atcactcggg cgcacgccag
     2461 ctcaatgaat cggcgaccgg ctattttctt gctgcaacgg accagatgaa tcggggccag
     2521 ggatataaca tcttcgccga cgccttctca gatcaaccgg aggtgatgct tcccgacggc
     2581 agcaagctgc gtggacgcca ctacctcatt gagagcctgg aggaagacat ggacgccggg
     2641 atcaagatgc atctctccca cgtctacgcg agcaaaaccg tcacggtcct ggaagccacc
     2701 tttgaaaacc ctgccagcaa ccccacccac tgtcctcccg caactacaca ggtgcacttt
     2761 aagcgcgacg gcaaggcaga ccggatacgg ctgtatttcg cacctcgtcc agccacggaa
     2821 caagggaagg gaccggtcag cgaggaagtt aaccaatcgt aaatggaatg atgtgttcca
     2881 ttgatgacta caggaggaac aatgatgcac ggtaagcagg aatccatccc ggtcgttttc
     2941 gaacaggacg gtgttgtcag tcggcaagcc cagtggggtg agatgaccgt cgccttcgag
     3001 cgtgcgccag ttggcacgga cacccgtccg ctgttccagg ggcttcccgg cgacagctgc
     3061 cagtgccccc actggggtta tgtcattagc ggccagatgc gggtcatcta ccccgatcgt
     3121 gaggagatcg tcggcaccgg ggaagcgtat tacctcgctc ccggtcacaa tatcgtctgc
     3181 gacgcaaaag gcgaagtcgt cgagttcagc ccccatggcc aataccagaa gaccatgcag
     3241 gcggtggcta ccagactcgc gactaatgag gacaaggatt cttgatcggc gacaagtcaa
     3301 agtgcactga tggcggtttc gcttaccgga ccgaaatctc ccatgcgaag gagttcaaat
     3361 actcgttctt ctagatccgc tggtttcact gggtatgaat cgcctgtcat tcgcgtcagt
     3421 actcgcgaat gacaggcgcc atcgtttacc ccgcgcgagt ggcagtcctc ccgcccaccc
     3481 cgcacccggc aatctctccc cgggttgata agcacagcga catcgagttc cccattaaca
     3541 gctcccgaca ttcctctcgc gcttggcacc agtctgctcc gggccgcggt acactggccg
     3601 ggatcttgca gtgcaagctt cggcgttcca tcgcgagcca acacggactt cacacttgca
     3661 cgcggcgacc acttcctgtc gcaccgctgg accatgtaat gaaccaggaa accagaacta
     3721 acagttcatc cagctcctca gaatcgaaaa acggtcatga ggcgacgcag cctttgcccg
     3781 tcgacgccac acctcatctc cagctcgcct ccgagttggc gggtatgctg acgtgggaac
     3841 tcgacctcga caaagggacg gtcaggtggt caccagacgc cgagcgcgtg ctgggccgga
     3901 agccgccggc ctcgctggcc agtatcctcg cctgcgttca ccccgatgac agggaagtcg
     3961 ccaggcagca gttcaacgac ctggcacgcg gtaacaaccg gatagacatc accgcgcgca
     4021 tccccgaccc ggcacggcaa cgtgacaggt ggttccattg ccgcggcgcc cggttccatg
     4081 acgacgcaac aggatgcgac cgaatacttg gtatcgcgca ggacgtcacc gggtacaagc
     4141 agcgtgagct gaatgtatcc ttccttgccg aaatgcgcga gcacctggcc gccgccggaa
     4201 ccgaggatgt ccttcaggtc ttcggcgaaa ggacactcac ccacctcagc ctctcccgcc
     4261 tcaccctctc cgcactctcg gatgagaagg atagactcct ggtgctgcac gaccagcacc
     4321 tgaccgggaa tccgtgcgag aagatccctg ggctcacggg ttccttgctc ccggatatga
     4381 aaggcgccct gctgggtggt caaccggtcg cgatcgagga cgtggacagg gatcccctca
     4441 ccgcgcatat ggccgatgag ttccatggat gcggagtagg ctcgctgctc tacgttcctt
     4501 atcccgctac cggccaatgg cacatgctcc ttggtgcctg caaggactcg ccctacacct
     4561 gggacacggg cgagattgtg ctcctgcagg atttggcgga gcgactctcc ctccggttcg
     4621 agcacgtgcg cattgtgcag gaactgcggc aaagtgagga gcagtatcgt gcgctcttcg
     4681 actccattga tgagggcttt tgcatcatcg agatcctgct cgacgaagcc ggtgtgccga
     4741 cagactatcg cttcctcgag accaacccga tgttcgagca gcacaccggg ctgcacaatg
     4801 cgcttggcaa aacggccagg gagttggttc ccgaccttga tgagcactgg tttgagattt
     4861 acgggaaagt tgctctcacc ggggagcccg tgcgcttcgt ccaacacgca gccgccatgc
     4921 agcagagatg gtttgatgtc aacgcattcc gcatcgggga tcccgggcaa cgccgggtgg
     4981 ccattctctt caaggacatc tccgagcaga aacggatcga gcagacgctc cggcagtcgg
     5041 aggaacgcct tcgacgtgcc atcgagattg agacggtagg catcatcttc ttccaccccc
     5101 agggccaggt tatcgacgca aacgaggcct ttctccgtat gagcggctat acccgggatg
     5161 atctggagga ggggctcctt cggctggaca cgttgacgcc gccggagtgg atgccggaat
     5221 cccgccgcgc tatcgacgag tttctctcgc acggtcgcat tgcaccctat gaaaaggaat
     5281 atctccggaa ggatggatcg cgctggtggg gcctctttgc tgccgcgcgt gttggcgaca
     5341 acgagggcgt caagttcatc atcgatatca cagcatcccg gcaagcggaa gcagagcggg
     5401 cacgcctcgc cgatatcgtc gacagctcac gggatgccat tatcctctgc gatccggacg
     5461 gaacgatcat cgactggaac ggagcagccg aagagctcta tgggtacacc tctgatgaag
     5521 tcatcggcca acacatccgg ctcgttgttc ctccagatcg ccggcacgaa tccaggggag
     5581 ccttcgagcg actggtggat ggtgagcagg ttcccccttg ggaatccgtg cggactcgca
     5641 aggacggcag caccgtctac gtagagatcc ggctgtcacc gattttcggc ccacagggga
     5701 aggtcgtagg agcatcggcg attgtccggg acatcagtga gcgaagacgc cttgcccagg
     5761 cccaggatga cttcctcgcc atggcaagtc acgaccttcg ctcgcctgtc accgtgcttc
     5821 tcggccgggc ccagctgatg cgccgtcgaa agacgtacga tgaagcgagc atcgagacga
     5881 tcattaccca ggcccgtcgc atcgaacgct tagcaatgga tctgcagcaa gtagtgcaaa
     5941 tggaatccgg ccagctccag ctcaattgct ccccggtgag cctcggaacg ctcgcccggg
     6001 aggcggttga tcgcgtccgt tcgatcaccg acgcctttga ttttcgcgtc gacgtgcctg
     6061 aggatcctgt caccggaaac tgggatgctc atcgtctcaa ccagatcctc gataacctgc
     6121 tgggaaacgc cgtgaagtat tcgcctgatg gcggaaggat caggattgcg gtctcggaga
     6181 acggggagag cgcccgcatt caggtaaccg accaaggtgt tggcatcccg gatagcatgc
     6241 agaaaatgct attcacccgc ttctaccgtg ccgatgaggc cggagtggca tcgggcctcg
     6301 gtctcggcct ctacatctcc cggatgctcg ccgaagctca tggcggctcg attagcgtcg
     6361 actctgcacc cggtgcagga agcacgttta tcgtcgattt gccgtttgct atgccgggaa
     6421 ggcaggcata acggcctgcc ttcccgtcgt tgttgtcctc atgtccctgc tcgccggacg
     6481 gacccggaga cttgagtcac gctcacaatg tctggcaata ttggtcccgc tgagaccgtg
     6541 tcgtgcctgg tcgcgcgagc gaacgaaact gaccgcgatg cactgcgctc caaaaggaca
     6601 ggaatgccag tcgagataac agcactccct ggcgtgacga ctgcttcagg ggacatcccc
     6661 gtgtcgtcac gccgggtaga agccggtgcc acacgcctcc ggctggtgca tcctctcatc
     6721 tcgactgctc ttttcttcct ggcgtgctgg cacttcttcg gagcgatcga tgaccggccc
     6781 atgcaccgcg atgaggcgcg ctggattcac agggccgcct atatccgtga gctgatgcac
     6841 ccgttcagca gctattggga cgaggaaacg tggattgcca aaggcggcac cctggacgag
     6901 cgataccggc ttcgcgccca gccgcccatg ggctcctatg tgatgggaat cggattcctg
     6961 ctgcagggac aggacttgcc agatatcggc ttttggaata tggaccagga cgatgcctgg
     7021 aacatcaagc gcgggaatca gccatcacct tcggatgtca ccaccgcgcg acgaaccagc
     7081 gcgatcgtcg gcgccttgac agtcgtctcg gtctatttca tcgccagccg cctcacgaat
     7141 cccgtcggca gcctgatggc gggtatcttt ctcgcctttc atcccctgat gagtcatctc
     7201 gccacctttg ccgggtccga tgcggcgctc gggctttcca tcgccgctgc cgcaatcgcg
     7261 gcctaccgtc tcgccgaccg gccatcgtgg ctccgggcga tcctgctcgg cgtgatcctg
     7321 gggttgggtg cttcgaccaa gctcagcccg ctcggtgtca ccgcaccgct ggcgcttctg
     7381 ggtgtcatcg cggtcgccta ccggctcctg ctgcttgttc gcgaaaccag gggcaacaag
     7441 ccgccatcgg ctgaatcagg acgaaacaca tggccgtcat ggaggctggg cttgggcctg
     7501 ctctccacac ctgtcgttgc cctgttcacg ttcatcgcca gctacccgta tctctggcaa
     7561 gatccggtcg cgaatattca ggcgctggtc gactatcgac agtggggtat ggacatccag
     7621 ggcatgctgt gggagcacat cgccgtcgag acacgaacgg aggcactcca gcgcgtcggc
     7681 gcccggcttg gcaccgagtg gacgaccttg ggtcgcctcg tggagcgatt tgggtggagc
     7741 tggattcctg ctgggttcga gttaaccctt gccgccgccg gcctcgtact cctcctctgg
     7801 ttggtggctc agcgaggagt ttggagcggc ccggcgctca ccgcgacggt cctcaccggg
     7861 caggcagcga tcacgatcta cggcctcggt gtagactggg cgcgctatca cctccccgtc
     7921 ctgctgctcg tcgccgtctg catcggtgtt cttgccgggg aaataatggc cggactgggg
     7981 cgactgccta gcttcaggca ccccagcccg ggaatacctc atgaactacc gccgccaccg
     8041 gattcccccg cggatcttga cggccgctga gctccggtcg ctgtcgctcc accggcgcca
     8101 caacagcgcc agtggcacga ccgccatacc cacctttgcg gcgcgagtgc ccttcggaga
     8161 gccgtccttc cacccgcccc tcacgtcatt gagggcaggc attggcgcgt agaggttgcc
     8221 tgtagttgaa ggtgaaggtg tgtccgccag atgctcccgg t
//
LOCUS       DASYWM010000274        13587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_669548, whole genome shotgun sequence.
ACCESSION   DASYWM010000274 DASYWM010000000
VERSION     DASYWM010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13587
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669548"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1300
                     /locus_tag="VGR29_11190"
     CDS             65..1300
                     /locus_tag="VGR29_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2074192.1"
                     /translation="MAASIASRTDVFFQERREIERQRLWKVLVGAAIVALYPLIDSAL
                     GLGLLGSWIPILVYVLLALGLNIVVGYAGLLDLGYVAFFVIGAYAQAFLTSPSSVFVQ
                     KGWVPEFMQNFWPAMAVSWVVAAICGAIIGAPTLRLRGDYLAIVTLGFGEIVPDFFTN
                     TVSVTRGPQGIGQITKPPTIPLPFGWSIGFGTQNQWQWFWLILFVGLLSLFVITRLYD
                     SRMGRAWQAIREDEIAAASMGINPVRTKLWAFALGASFSGFAGSLYSGYVGVVYPDQF
                     QFSLSIVILSMVILGGIGNIYGVLAGALIIGTFDRILAEKLNGPMNWLGEQITWGPVA
                     PIGNFLANHSLTSDRFLIFGLALVLIMLLRPGGLFPSARRAAEMKGEDNNYTFDEDEA
                     DQSLREAREGDVGNREGDI"
     gene            1303..2229
                     /locus_tag="VGR29_11195"
     CDS             1303..2229
                     /locus_tag="VGR29_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2074193.1"
                     /translation="MATTTSPTQATSVSSRETALQARGVTKRFGGLVAVDSVDLQIAK
                     GSISSVIGPNGAGKTTFFNMIAGIYRPTEGDIYFLDRPISSESNRRGSLRPHEVTDIG
                     IARTFQNIRLFANVSAIENVLMGMHTRLTANPLGAILRTRSVRQEEYEAREKAFSLLD
                     YVGLGEQARTTAKNLSYGDQRRLEVARALASDPKLLLLDEPTAGMNPRETEGMTAFID
                     RMRHEFDLTILLIEHDMKVVMGISDHIAVLDHGMKIADGTPREVRENPKVIEAYLGAG
                     SAEVIARLSGEQHAEDGTSIIARPLDVGDGAK"
     gene            2241..2942
                     /locus_tag="VGR29_11200"
     CDS             2241..2942
                     /locus_tag="VGR29_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2074194.1"
                     /translation="MLEMVDVHSYYGHIHALKGISLDVHQGEIVTLIGSNGAGKSTTL
                     RTISGLLTPRDGEVRVLGQKISGMPAHKVSSMGIAQSPEGRRVFPRMTVLENLQMGAF
                     VNGKVDQADLARVFDLFPRLKERTSQNAGTMSGGEQQMLAIGRALMARPKLLLLDEPS
                     MGLSPILVEQIFSIVKDINQQGTTVLLVEQNALMALAIARRGYVLQTGNIVLTDDAKA
                     LMANETVRKAYLGEE"
     gene            3247..5418
                     /gene="glgP"
                     /locus_tag="VGR29_11205"
     CDS             3247..5418
                     /gene="glgP"
                     /locus_tag="VGR29_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEV2074195.1"
                     /translation="MSFKMRASRVDLPERIDRLNELSYNLWWTWQPRARALFRELDPV
                     LWGVCKENPVLFLHRIDPARLSRAVEDPAFLGQYDDVMMRFDTMINASDSETWLAHRH
                     PELLDKKVAYFSAEFGLHRALPIYSGGLGVLAGDHIKEASDMGIPLIAVSLLYRQGYL
                     QQRIDSSGWQHDVPAHLEPDSEPTTQVLNEDGTPVLIEVDLQDGPGSLCLAVWQVKVG
                     RVSLYLLDSDVGGNPEWTRQLSSRLYGGDTEHRLRQEIVLGIGGVRLLRALGIEPTYW
                     HANEGHAALSMLERLREYVHQGKSFEEAQDLVRASTVFTTHTPVPAGHDIFSPEMIDR
                     YFSSYWPELGLDREEFLALGRHGDDPGFNFTALSLRLSAHRNGVSEKHGEVSRQMWHD
                     IWPDTGPENAPIQSITNGVHLRTWISPAMRRVYSRYQPRYWKDQQEKQHVWDTILNIP
                     DDEFWAAHQQAKQALIAEMHERSRKRYVEGQFDAWQVLSSGPFQNPNVLTLGFARRFA
                     TYKRATLIFRDIDRLARILTNAEHPVQLIFAGKAHPADEGGKKFIQDIYLKARDPRLG
                     GRVAFVEDYEMGLASLLVAGVDVWLNNPRYPLEASGTSGMKAAANGVPNFSILDGWWI
                     EGWEPGNRNGWGIESSPRQGDAQDEAEANMMYEMLEEQIVPLYYQRQADGLPHDWIQV
                     AKEAIRTVAPRFSARRMLIDYVEKLYAPAAGVAAREEVAAR"
     gene            5560..6699
                     /locus_tag="VGR29_11210"
     CDS             5560..6699
                     /locus_tag="VGR29_11210"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEV2074196.1"
                     /translation="MSETQLDEMQIPSQLPSADVRLRPLKGLILAGGKGSRLRPLTAT
                     GAKQLVPVANKPVLFYALEQLVEAGIEEIGIITGDSGGQVREAIGDGSQFGAQITYIP
                     QDAPLGLAHAVLTGREFLGDSPFCMYLGDNFLRQGIVGHAEQFRHSDAAGQILLKKVD
                     DTSALGVAVLDANGKVQQLVEKPKIPISPYAVVGVYFFGPEIHDVVPGLVPSARGELE
                     ITDAIQGLIDAGNEVDASLIDDVWIDTGKKDDMLEANRVVLSGITRRIDGEVDSASVL
                     VGEVIIEEGAEIRGSSVRGPAIIGKGARLDGAFVGPFTAIGDRVHLQSCEIEHSIVLA
                     DSSIKNISRRISDSLIGREVVIQRTDHRPHVMRLMIGDHGQLDIS"
     gene            6737..7636
                     /gene="rfbD"
                     /locus_tag="VGR29_11215"
     CDS             6737..7636
                     /gene="rfbD"
                     /locus_tag="VGR29_11215"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV2074197.1"
                     /translation="MTGAGGQLGRYLCTELRDAGYDVIRLGHAEGNDIDAVVDISDAN
                     AVHDVFGDSPPDTVIHAAAYTDVDGCEKNRELANQVNHLGSRNIAEASKRAGAWLLAV
                     GTDFVFSGESDEPYAEDAEPGPISVYGQSKLDGERAVLATEDSFAVARTSWVFGGEGK
                     HFPRTVVSMLQERGAMSVVDDEVSSPTYAGDLARALVALVPLRPSGVLHLSNEGVVSR
                     YQFAREVARIAGLDPRSVTPTSTAEFLAKYPLPAKRPAFSALANERAAAIGVRLPAWE
                     DAVSRYIPRMMSAWSQPSARGQS"
     gene            7558..8667
                     /gene="rfbB"
                     /locus_tag="VGR29_11220"
     CDS             7558..8667
                     /gene="rfbB"
                     /locus_tag="VGR29_11220"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV2074198.1"
                     /translation="MGGRRVAVYTPNDVRLEPTVSERTIVKILVTGGAGFIGSAFVRL
                     VLERYPDDTVTTLDALTYAGNPANLFELEQNPRHSFVHGDIADVSLVDGLVAGHDAVV
                     NFAAETHVDRSLLDPSAFLRSNVEGVYVLLDAMRRQGGRLVQVSTDEVYGDIPEGKFS
                     LESDPLAPRSPYAAAKAGGELFCRSFSVSYGTDVVVTRGCNTIGPRQYPEKLVPLFIT
                     NALEDRPLPVYGDGLQVRDWLHAEDHAAGIDLALRRGRSGEIYNIGAGNERPNIDVVR
                     QLLAHLGKSESLIAYVPDRPGHDRRYALDSNKIRTHLGWAPQRDFESTLGDTIDWYLS
                     HPEWWRAIRNASGEFAEYYERQYGWRLAAAGRDEG"
     gene            8672..9265
                     /locus_tag="VGR29_11225"
     CDS             8672..9265
                     /locus_tag="VGR29_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV2074199.1"
                     /translation="MTGEYSSKTLDAVESVLVRDGGTAPSIFDVTVRSLRTNRDPRGT
                     LTELLRTDWSDVYGDSMPFAQVYTSTTEPGVARDIDTWHVHQNQTDRFYCVSGRIVVA
                     IADPRDDSLSRGKLMLVELSAQEDAPAPLMVTIPARTLHGFVVTSEGPATLLNFPTQL
                     YDPQDEGRMPFAEANITFPDGSPFDYRAVSTWYSSKR"
     gene            complement(9298..10722)
                     /locus_tag="VGR29_11230"
     CDS             complement(9298..10722)
                     /locus_tag="VGR29_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HEV2074200.1"
                     /translation="MTHLSLRHLVFLMLIAMSLTVATGSVAAQRDPLASVVDMGSPQL
                     ETVPPTIFAREQRDSIAGVVNEARLFGVPLVVRVIHTPTPLGLLSEAGAIAGAAPSAQ
                     EITQRMAEEWLATEGVGTSPGAEDGILLLVVIPEDNHQLTTAAFATGDNALPLNGLTQ
                     DRLDHVITTIMPPRFADNTIAAGVHSGIAHLSYDNLFAVPARLQPTEQQERLRDLTNT
                     ALLGMTALGIIALGGLIFWIRRRDSARIPADADTLSPFEAGALARGRVDEPVVTAALL
                     HMIDCGILKASMGHAGVLALAMGHDIATVDPFEQQIMNLLAIEAGQDGRLPNAAVRRV
                     QDVLAPAREWLQDDLATRNLFNRDAKVETVWIILASLALGTLALFSLVPSVVAMSRYG
                     LFTFGIAMLVVAGALAWAARRSWTTSAGEQVLSAWLSSRSHPEEAAIYDTIVHQDALL
                     AAPGGPDTDPAVRMVRELRTLGAG"
     gene            complement(10719..12242)
                     /locus_tag="VGR29_11235"
     CDS             complement(10719..12242)
                     /locus_tag="VGR29_11235"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEV2074201.1"
                     /translation="MRQSLRQTRPPAIQHSLLKRSRRRLRYPRPRSFRPIAWPLVLRG
                     RNTEPRDRFLTSRDRILPDVLLALLFFALSLAVNLGALPTTPTHPDETRWMNRAYYAR
                     DLADPFGPTWQDYVTTRGQPPLGSIVMGIGLAIQGRDLDATGVWDFAYGSDWNERAGA
                     VPTEEDRHAARRTNAVIGAMVAGLAFVLGRLLTNRVGGGIAAGFVAFHPLHITLSTQA
                     LSDQTLALLLMLIFITGWSFARKPTWTRAILLGILLGLGGSVKLSPLLLSAPLALFGV
                     MRLVVDRDRSGVDYALKLLAQPVVALAMFVASYPYLWPAPIRRTWNLYAFRASEMEGQ
                     SEAWPEVAVASPLDALGRFGRYLTDDRSVTGSIIQFVYDGLGIDRAATGLDYIPAVAG
                     IVILIWWIAKRGLWTPAAMVGLLMGAEAGALVFGMKTDFYRYHLPIVVIMAGCIGIGT
                     GTAWSALSHYLRSREVQARRAPTAAARTSIQPLRPTTRNRPSPVRAFEAARAREARQ"
     gene            complement(12239..13177)
                     /locus_tag="VGR29_11240"
     CDS             complement(12239..13177)
                     /locus_tag="VGR29_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074202.1"
                     /translation="MRGTALLTVLMLYFLLPGVPEVMAAQAQPDPPPETLPYFYDPSD
                     VFPEDQQVTLTRDARLMQRSGIPSVVYVRTATAGEAGKESSRAFADTVRREWNVESSP
                     DADDGLVILLSYVPDNPAASTAVASWGTSTFHDSGLTPEYVQSVLDRDVRTLLDMEFP
                     FEAMVYGLRQIRYGGIYFPPPPDPLEGGAKTLHDVLRWVGPVLTIGMVSAFVALSLRR
                     HADKHMAPKFLWKTAGLTGMLVVMLSVLSVIGRSRVGIGSALLILVALAIQAWLWSHP
                     PRQQARHIERRTVPPTSQRLRKRRQARRMLATGMRR"
     gene            complement(13242..>13587)
                     /locus_tag="VGR29_11245"
     CDS             complement(13242..>13587)
                     /locus_tag="VGR29_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074203.1"
                     /translation="AWSSTEEFLATIGLPALGDRLSMLDVVLATIGILLLLGVGLRKG
                     LRSAELAIAGLVIFQTATIIMTMRVDFERYYLPILLGMVVAIGYSVGYAAYGVQKLVT
                     RNDSPAADTARP"
BASE COUNT         2749 a         3855 c         4245 g         2738 t
ORIGIN      
        1 ccgccggctt gttcggccat tcagcacctg agaaggtctg agcaggcccg aaggagagac
       61 cagtatggcc gcatccattg cctcacgaac cgatgtcttc tttcaggaac ggcgtgagat
      121 cgaacgccag cgactgtgga aagtgctcgt cggcgcggcg attgttgccc tctacccgct
      181 tatcgactcc gcgctggggc ttggtctcct cgggtcctgg atcccgattc ttgtctatgt
      241 cctgcttgcc ctcggcctca atatcgtcgt cggttacgca ggtttgctcg atctcggcta
      301 tgttgcgttc ttcgtaatag gcgcctatgc ccaagcgttc ctgacgtcgc cgagtagcgt
      361 tttcgtgcag aaaggctggg taccggagtt catgcagaac ttctggcccg ccatggcggt
      421 ctcctgggtg gtcgctgcga tctgcggcgc gatcatcggt gcgcctacac ttcggcttcg
      481 cggtgactat ctagccatcg taacgctcgg tttcggggag atcgttcccg actttttcac
      541 aaataccgtc agcgttaccc ggggtcccca aggcatcggc cagattacca agccgcccac
      601 gattccgctt ccattcggct ggagcattgg ctttggcacc caaaaccagt ggcaatggtt
      661 ctggctcatt ctgtttgtgg gccttctctc gctctttgtc atcacccgtc tctacgattc
      721 ccgaatgggg cgtgcctggc aggcgatccg ggaggatgag atcgcggcgg ccagtatggg
      781 gatcaacccg gtccggacca aactctgggc cttcgcgctc ggcgcctcgt tttccggttt
      841 cgcaggttcg ctctactccg gttatgtcgg ggtcgtgtat cctgaccaat tccagttctc
      901 gctctcgatc gtcattctgt cgatggtcat ccttggcgga atcggtaaca tctacggcgt
      961 cctggccggt gcgctgatta tcggaacctt cgaccgcatc ctggcggaaa agctcaacgg
     1021 gcccatgaac tggttggggg agcaaatcac atgggggcca gtggcgccca tcgggaactt
     1081 cctggccaat cacagcctca catcggaccg cttcctcatc ttcggtttgg cactggtgct
     1141 tatcatgctg cttcgtccgg gtgggctctt cccgtcggcg cgtcgtgctg cagagatgaa
     1201 gggcgaagac aacaattaca ccttcgacga ggatgaagcc gaccagtcac tgagggaagc
     1261 ccgcgaggga gatgtaggaa atcgcgaggg ggacatttag gtatggcgac gacaacttct
     1321 ccaacgcagg ccacatcggt ttcctcgagg gagaccgcac tccaggcacg gggggtgacc
     1381 aagcgcttcg gcggcctggt tgccgtcgac agtgtggacc tgcagatcgc caaggggtcg
     1441 attagtagcg tgatcggccc gaatggcgcg gggaaaacca cattcttcaa catgattgct
     1501 ggtatctacc ggccgaccga gggagatatc tactttcttg accggcccat ctcgtcggag
     1561 agcaatcgcc gtggatcatt acggccccac gaggtgacgg atattggcat cgcaaggaca
     1621 ttccagaaca ttcggctctt cgccaatgtg tcggccatcg aaaatgtatt gatgggcatg
     1681 cacacacgtc tcaccgccaa tcccctgggt gccatcctga gaaccagatc cgtcaggcag
     1741 gaggagtacg aagctcggga gaaggccttt tcgcttctgg attatgttgg cctaggtgaa
     1801 caggcgcgta caaccgcaaa gaacctctcc tatggcgatc agcgccggct tgaggtggca
     1861 cgggcactgg caagtgatcc aaaactgctg ctgctggatg agcccaccgc cggcatgaac
     1921 ccgcgtgaaa ccgaaggcat gaccgcgttc atcgacagga tgcggcacga gttcgaccta
     1981 acgatccttc tcatcgagca cgacatgaag gtcgttatgg gcatctccga ccatatcgcc
     2041 gtgctggatc atggaatgaa gatcgccgac gggacgccca gagaggtccg ggagaacccc
     2101 aaggtgattg aggcctatct tggtgcaggg tcggctgagg ttatcgcccg gttgtccggt
     2161 gaacaacatg ccgaggatgg aaccagtatt atcgcgagac ccctggatgt tggggatgga
     2221 gcgaaatgaa taaccagaag atgctggaga tggtcgatgt ccactcctac tatggccaca
     2281 tccatgctct gaagggaatc tcgctggatg tgcatcaggg ggagattgtg accctgatcg
     2341 ggtcgaacgg tgcaggaaag agcacaaccc tgcggacgat ctcgggcctg ttgacgccgc
     2401 gcgacgggga ggtacgcgtg cttgggcaaa agatcagtgg gatgccggca cacaaggtct
     2461 cgtcgatggg aatcgcgcaa tcaccggagg gtcggcgcgt cttcccccgg atgaccgtgc
     2521 ttgagaattt gcagatgggc gcgttcgtca acggcaaggt ggaccaggcc gatctcgccc
     2581 gggtattcga cctgttcccg aggcttaagg agcgcaccag ccagaacgcg gggacgatgt
     2641 ccggcggtga acagcagatg cttgcgatag gccgggcgct catggctcgt ccgaagctcc
     2701 tgctccttga cgagccgtcg atgggcttgt cgcccatcct tgtcgagcaa attttttcaa
     2761 tcgtcaagga catcaatcag cagggaacga ccgtccttct cgtcgagcag aatgcgctca
     2821 tggcgctcgc aatcgccagg cggggatacg tccttcaaac aggaaacatc gtgctgacag
     2881 acgatgcgaa ggcgctgatg gcaaatgaga ccgtgcgtaa agcctatctc ggcgaagaat
     2941 aaactgatgg gaagtggcag ctctttgaat gtacaggcga cgatgccttt gacagagccg
     3001 ttcggtcccg ttgcacaaga acttgctacg tcctcactga tgtagcccgg cagcgtgtta
     3061 tgtcggaatc tggtataccc ctactgaatg cgcttcctga tagagcgcag ccggaaccaa
     3121 gttgcaaagc acccgtcacc cactggtggg cgccttccgg tgaaatgggg aatctatcgc
     3181 tggacatgtt cgttcagctg gtactgcggc tggtcctcca gcacgaaatg aacgaggcat
     3241 caccatatga gcttcaagat gcgcgcttcc cgggttgatc tcccggagcg cattgatcga
     3301 ctaaatgagc tttcctacaa cctttggtgg acctggcagc cgcgagcccg ggcgctcttc
     3361 agggagttgg acccggtgct ttggggtgtt tgcaaggaga acccggtact cttcctgcac
     3421 cggatcgacc cggctcgcct gagcagggcg gtcgaggatc ctgcattcct gggtcaatat
     3481 gacgatgtca tgatgcggtt cgacacgatg atcaacgcgt cggacagtga gacctggttg
     3541 gcgcatcgac atccggagct tctggataag aaggtcgcct atttttcagc ggagttcggc
     3601 ctgcaccggg cgctgccgat ctattccggt ggcttgggtg tgctcgctgg cgatcacatc
     3661 aaggaagcta gcgatatggg cattccgctc attgcggtct cgctgctgta tcgccagggc
     3721 tatctgcaac agcgcataga ttccagcggt tggcagcacg atgtaccggc tcatctcgag
     3781 ccggactccg agccaacgac acaggttctg aacgaggacg gcacgccagt cctgattgaa
     3841 gtcgatctcc aggatggacc agggtcgctc tgtcttgctg tctggcaggt gaaggtgggg
     3901 cgagtctcgc tttacctgct tgactccgat gtcggtggga accccgagtg gacccgtcag
     3961 ctctcatcgc gactctacgg tggcgatacg gagcatcggc ttcgacagga gatcgtcctc
     4021 ggcattggag gtgtccgcct gctgcgcgca cttggtatcg agccgaccta ctggcatgcc
     4081 aacgaggggc acgccgcgct ctcgatgctg gagcgattgc gcgagtatgt ccaccagggc
     4141 aagagtttcg aggaggctca ggacctggtg cgggcatcga cagtctttac cacgcacacc
     4201 cccgttcccg cagggcatga catcttctcg ccagagatga tcgaccgata tttctcaagt
     4261 tactggccgg aactcggttt ggatcgcgag gagttccttg cgcttggtcg tcacggcgat
     4321 gatccaggat tcaacttcac agctctgtcg ctacggctgt ccgcacaccg taatggcgtg
     4381 agcgagaagc atggtgaagt ctcccgacag atgtggcacg atatctggcc ggacacggga
     4441 ccggagaacg caccaatcca gtcgatcacc aacggtgtgc acctcaggac ctggatctcc
     4501 ccggccatgc ggagagtcta tagccggtac cagccccggt actggaagga ccagcaggag
     4561 aagcagcacg tttgggacac catattgaat atcccggacg atgaattctg ggccgcgcac
     4621 cagcaagcga agcaggctct gatcgccgag atgcacgaac gttcccgtaa gcgctacgtg
     4681 gagggtcagt tcgatgcgtg gcaggtcctt tcgtcggggc cattccagaa cccgaatgta
     4741 cttacgctcg gcttcgcgcg acgattcgcc acctacaagc gtgccaccct aatcttccgg
     4801 gacattgacc gccttgcgcg gatcttgacc aatgcggaac atccggtgca gctgatcttc
     4861 gccggcaagg ctcaccctgc cgacgagggc gggaagaaat tcattcagga tatctacctg
     4921 aaggccaggg atcctcgttt gggaggtcgg gtggcgttcg tcgaggatta cgagatgggg
     4981 cttgcaagcc ttctcgtggc cggcgtcgat gtatggctga acaacccacg ttatccgctc
     5041 gaagcatccg gcacgagtgg catgaaggcc gcagcaaatg gtgtgcccaa tttcagcatc
     5101 ctggatggtt ggtggattga aggttgggaa ccgggaaaca ggaatggctg gggcatcgag
     5161 tcatcgccca ggcaaggtga tgcccaggac gaggcggaag cgaacatgat gtacgagatg
     5221 ctcgaggagc aaatcgtgcc gctttattac caacgccaag cagatgggct tccccacgac
     5281 tggatccagg tggccaagga ggcgatccgg accgtcgctc cacggttcag cgctcgccgg
     5341 atgctcattg actacgtaga gaagctctac gcgccagcgg cgggcgtggc agcccgcgaa
     5401 gaggtggcgg ccaggtagcg cctccgcttc cagccataca tcgtggaagg actggtctcg
     5461 cgcgtgatga aggtcatgtc gacggccagt cttttttgcc aaggaactta aactgtgcct
     5521 cgtgttcggg atactattgc gcgttggagg ggctgaacca tgagcgagac ccaactggat
     5581 gaaatgcaga tcccatcaca actcccgtca gccgatgtgc ggttgcgccc gctgaaaggg
     5641 ctgattcttg ccggcggtaa ggggtctcga ctgcggccgc tgacggcgac cggcgcaaag
     5701 cagctggttc cggttgccaa caagcctgtg ctcttttacg cgcttgagca gctcgtcgaa
     5761 gcaggcatcg aggagattgg catcatcacg ggtgacagtg gcggccaagt ccgtgaggct
     5821 attggtgacg gttcgcagtt cggagcccaa atcacctata ttccccagga tgcgccgctg
     5881 ggtctggccc atgcggtgct taccggccga gagttccttg gcgactcacc cttttgcatg
     5941 tatctcggag acaattttct gcggcaggga atcgtgggcc acgccgaaca gttccgccac
     6001 agcgacgctg ccggccagat actcctcaag aaggtagatg acacgtccgc actcggtgtc
     6061 gctgtgctcg atgcgaacgg aaaagttcaa cagttggtgg aaaaaccaaa aatccccatc
     6121 tcgccttatg cagtcgtcgg tgtttatttc tttggtcctg aaattcatga cgtcgtcccc
     6181 ggattggttc cctctgcccg tggtgaactc gaaattaccg atgctatcca ggggttgata
     6241 gacgccggca atgaggttga cgcctcgctt atcgatgatg tctggatcga cacgggcaag
     6301 aaggacgaca tgcttgaggc aaaccgggta gtcctgagcg gtattacccg gcggatcgat
     6361 ggcgaagtcg atagcgcctc cgttctggtc ggtgaggtca tcatcgaaga gggtgcggag
     6421 atccgcggca gttccgtgcg tgggcctgcc atcatcggca agggagcacg tcttgacgga
     6481 gcctttgttg gtccctttac ggctattggg gatcgggtgc acctgcagag ctgcgagatc
     6541 gaacattcga tcgtgctcgc agatagttcg atcaagaaca tcagcaggcg aatcagtgat
     6601 tcccttatcg gccgagaggt ggtcatccag cgaaccgacc acaggccgca cgtgatgcgc
     6661 ctcatgatcg gtgaccacgg ccaactggat atttcgtgat gcggagtagc gaatgaggcg
     6721 aggagagcga gtcctcgtga ccggagcagg tggccagttg ggcagatacc tttgcacgga
     6781 gcttcgggac gctggctatg atgtcatcag gctgggtcat gctgaaggca atgatattga
     6841 tgctgtcgtg gacatcagtg acgccaacgc ggttcatgat gtgtttggtg atagcccgcc
     6901 ggacacagtc attcatgcgg ctgcctacac agatgtcgat gggtgtgaga agaaccggga
     6961 gctggcaaac caggtaaatc atctcggttc gaggaatatc gccgaggctt caaaacgtgc
     7021 cggggcgtgg ctcttggcgg tcggcaccga cttcgtcttc tcgggggaga gcgatgagcc
     7081 ctatgccgag gatgccgagc cagggccgat ctccgtatat ggtcaaagca aactggatgg
     7141 tgagcgggcc gtgctcgcta cggaggattc attcgccgta gcaaggacat cctgggtatt
     7201 tggcggcgag ggcaagcact ttccacggac cgtcgtctcg atgctgcagg agcgcggcgc
     7261 gatgtccgtt gtcgatgacg aggtgagctc cccgacgtac gccggtgatc tcgcccgagc
     7321 attggtggcg ctcgttccat tacgaccctc cggggttctg cacctttcca acgaaggtgt
     7381 ggtcagcagg tatcaatttg ccagagaggt cgcgcggatc gccggtttgg acccgaggtc
     7441 ggtgacgccg acgagcaccg cggagttcct ggcaaagtac ccattgccgg cgaagcggcc
     7501 ggccttcagt gcgttggcca acgagcgcgc agctgcaatc ggcgtgaggc tgcccgcatg
     7561 ggaggacgcc gtgtcgcggt atataccccg aatgatgtcc gcctggagcc aaccgtcagc
     7621 gagaggacaa tcgtgaagat tctggtgacc ggcggcgccg gctttattgg ttcggcattc
     7681 gtacgcctcg tgttggaaag gtatccggac gacacggtaa cgacgcttga tgcactgaca
     7741 tatgccggga atcccgccaa tctgtttgaa ttggagcaga atcctcgcca cagctttgtc
     7801 catggcgaca ttgcggatgt ttctctggta gacgggttgg tagccggaca tgatgcggtg
     7861 gtcaactttg ccgctgagac gcacgtcgac cggtcattgc tcgatccgag cgcgtttctc
     7921 cggtcaaacg tggagggcgt ctacgtcctg cttgatgcga tgcggcggca aggtggtcgt
     7981 ctcgtgcagg tcagcacgga cgaggtctat ggtgacattc cggagggcaa gttctcgctc
     8041 gaatctgatc cgcttgcgcc tcgctctccc tacgcggcgg caaaggcggg tggtgagctc
     8101 ttttgccggt cattctcggt ctcctatggc actgatgtcg tcgttacccg gggatgtaat
     8161 acgatcggtc ctcgtcagta tcccgagaag ctcgtgccgc tcttcatcac aaacgccctg
     8221 gaggaccggc cacttccggt ttatggcgat ggactgcagg tacgcgactg gcttcacgcc
     8281 gaggatcatg ctgctggcat tgacctcgcc ctgcggcggg gccggagtgg tgagatctac
     8341 aacatcggcg ccggaaacga gcgcccgaac atcgatgtcg tgcggcagct cctcgctcat
     8401 ttgggcaaga gtgagagcct tatcgcctac gtgccggatc gaccggggca tgacaggcgc
     8461 tatgcgctgg attccaacaa aatccggacc catctgggat gggcaccaca gcgagatttt
     8521 gaatcgacgc ttggagatac gattgactgg tacctgtcgc atcccgagtg gtggcgggcc
     8581 attcgcaatg cctcggggga gtttgccgag tattacgagc gacagtatgg ctggcgcctg
     8641 gcggcggccg gtcgcgatga agggtaagtg gatgacaggg gagtattcat cgaaaacgct
     8701 ggacgcggta gagtccgtgc ttgtacggga cggcggcacg gctccgtcca tcttcgatgt
     8761 cacggttcgc agtctgcgaa caaaccggga cccacgtggc acgttgacgg aactgcttcg
     8821 tactgactgg tctgacgtct atggcgattc catgcctttc gcccaggtct acacctcaac
     8881 aacggaacct ggcgttgccc gcgatatcga cacatggcac gtgcatcaga accaaaccga
     8941 ccggttctac tgcgtttccg ggcgcatcgt cgtagcgatt gccgacccgc gagatgactc
     9001 gctctcgcgc gggaagttga tgctcgtgga actctccgca caggaggatg cgccggcgcc
     9061 gctgatggtg acgatccctg cacgcacgct gcatggcttt gtggtaacga gcgaaggacc
     9121 ggcaaccctg ctcaatttcc cgacccaact ttacgatccc caggacgagg gccgcatgcc
     9181 gttcgctgaa gcgaacatca ccttcccgga cggttccccc ttcgattatc gggccgtttc
     9241 tacatggtat tcgagcaagc ggtaaagcgt tttgccgggg gccggtcgat gactgcccta
     9301 tccagcacca agggttcgta gctcacgcac catgcgaacc gctggatctg tatccgggcc
     9361 cccaggagca gcaagcagtg catcctggtg gacgatggta tcgtagattg cagcttcctc
     9421 cggatgggat cgtgatgaga gccacgccga gaggacctgc tcgcccgcgc tggtggtcca
     9481 acttcggcgg gccgcccaag caagtgcccc ggccaccacg agcatggcga tgccaaaggt
     9541 gaagaggccg tagcgcgaca tggcgactac cgacggcacg agggagaaaa gcgcaagtgt
     9601 tcccaaggcc agactagcga ggatgatcca gacggtttct actttggcgt ccctattgaa
     9661 gagattgcgt gtggcgagat cgtcctgaag ccactcccgg gctggagcga ggacgtcctg
     9721 aacgcgacgc actgcggcat tggggagacg accatcctgt cccgcttcga tagcaagcag
     9781 gttcatgatc tgttgttcaa atggatcgac agtcgcaatg tcgtggccca ttgcaagggc
     9841 cagtaccccc gcatgtccca tcgaagcttt tagaattccg cagtcaatca tatgcagcag
     9901 ggccgccgtg accaccggtt catcgacccg tccccgggca agagctcctg cctcaaatgg
     9961 ggaaagggta tcggcatccg ctggtattcg ggcgctgtcc ctacgccgga tccagaaaat
    10021 gaggccaccc agggcaataa tcccgagcgc ggtcattccc agaagcgcgg tgttggtgag
    10081 gtctcgtaaa cgctcttgct gctcagtcgg ctgaagccgg gctggaaccg cgaagaggtt
    10141 gtcgtatgag agatgcgcga ttccggaatg gacgccggca gcgatggtgt tgtccgcaaa
    10201 gcggggcggc atgatcgttg tgatgacatg atctaggcga tcctgggtga gaccgttcaa
    10261 ggggagggcg ttgtcacccg tcgcgaatgc cgctgtcgtt aactggtggt tgtcttcagg
    10321 aataacaaca agcagcaaga tgccatcctc tgctcctgga gacgttccga caccctctgt
    10381 cgccagccac tcctcggcca tccgttgtgt gatttcctgg gcggagggtg ctgcaccggc
    10441 gatggctccg gcctcggaga gcagacctaa gggtgttggc gtatggatga cacggacgac
    10501 gagaggtacg ccgaagagac gggcttcatt caccacccca gcaatgctgt cgcgctgttc
    10561 ccgtgcgaag atggttggtg ggactgtttc gagttgtggc gaccccatgt caacgacgga
    10621 agcgagtggg tcccgctggg cagcgacgga accagtcgcg acagtcaagc tcatcgcgat
    10681 gagcatcagg aagacgaggt ggcgcaggga gagatgggtc actgccgtgc ctcccttgct
    10741 cgcgccgctt caaatgccct caccggcgac ggtctgttcc gtgttgtcgg tcgaagcggt
    10801 tgaatcgagg tacgagcagc agcggttggc gcgcgcctcg cctgcacctc ccgggagcga
    10861 aggtaatggg acagagcgga ccaagcagtt cccgtgccga tgccgataca cccggccatg
    10921 atgaccacga tcgggagatg gtaccggtag aagtccgtct tcataccgaa cacgagcgca
    10981 ccggcctccg cgcccatcaa gagcccaacc atcgccgccg gggtccagag tccgcgcttg
    11041 gcaatccacc atatcaggat gacgattccg gccacggccg gaatgtagtc gaggccggta
    11101 gcggcgcggt caatgccaag gccgtcatac acgaactgaa tgatgcttcc ggtcaccgag
    11161 cggtcgtccg tcaggtagcg tccgaaacgg ccgagagcat cgagcgggct tgccactgca
    11221 acctcgggcc acgcctcgct ctgcccctcc atctcagagg ctcgaaaggc atagagattc
    11281 cacgtccgac gtattggagc aggccagaga tagggatagc tcgccacaaa catggcaagg
    11341 gccaccacag gttgcgcaag gagtttgagg gcgtagtcaa cccctgatcg gtctcggtca
    11401 acgacgaggc gcatcacacc aaacagggcc aggggcgcgc tgagcaggag cggcgagagc
    11461 ttcaccgatc cgcccaggcc cagcaggata ccgagcagga tcgctcgtgt ccaggttggc
    11521 ttcctggcga aactccatcc cgttatgaag atgagcatga gcagcaacgc caatgtctga
    11581 tcggagagag cctgggtcga gagggtaatg tgcaggggat gaaaggcgac gaaacctgca
    11641 gcaattcccc cgccgacacg gttcgtcagg aggcgcccca ggacgaacgc cagtccagct
    11701 accatggcgc cgatgacggc attggtgcgg cgtgcggcgt ggcggtcttc ttccgtcggt
    11761 acggcaccag ctcgctcgtt ccagtcactc ccataggcga aatcccagac accggttgcg
    11821 tcgaggtctc gcccctggat cgccaaacct atccccatca cgatacttcc gagtggcggt
    11881 tggccgcgcg tggtgacgta gtcctgccat gttgggccga atggatcagc cagatccctc
    11941 gcgtagtagg cgcggttcat ccatcgggtt tcatccggat gggtcggcgt tgtgggaagc
    12001 gctccaaggt tcaccgccag cgacaacgcg aagaaaagca gcgccaggag aacatcgggc
    12061 aggatacgat cccgcgaggt caggaatcga tccctcggtt cggtgttgcg gccacgaagc
    12121 accaatggcc atgcgatggg ccgaaaggac cgggggcgcg gataacgcag ccgacgtcgg
    12181 gagcgtttaa ggaggctgtg ttggattgcc ggtggacggg tttgacgcag ggactgtctc
    12241 atcgtcgcat ccctgtcgcc aacatccttc gggcctgccg tcgcttccgt agccgctggc
    12301 tcgttggagg tacagtgcgg cgctcaatat gccgcgcctg ctgtctcggg ggatgcgacc
    12361 aaagccaagc ttggatcgcc agcgcgacta ggatcagcag ggcgctgccg atgccgaccc
    12421 tgctacggcc gatgaccgac agcaccgaaa gcatgaccac gagcatgccg gtgagcccgg
    12481 ctgttttcca gaggaacttt ggtgccatat gcttgtccgc gtgccgacga agcgacaggg
    12541 ctacaaacgc tgagaccatg ccaatggtga gtactggtcc aacccaccgc aggacgtcgt
    12601 gcaacgtttt agcgccaccc tcgagcggat cgggtggtgg agggaagtaa atgcccccat
    12661 agcggatttg tctcagccca tacaccattg cttcgaaggg aaactccata tcgagcaacg
    12721 tgcggacatc gcgatcgagc accgactgaa cgtattcggg cgtgagaccc gagtcgtgga
    12781 atgtcgatgt cccccacgag gcaacggcag tgctggctgc cgggttgtcc ggaacgtagg
    12841 agaggaggat tacgagacca tcgtcggcat ccggagagga ttcgacattc cattcacgcc
    12901 ggaccgtgtc cgcaaaggca cgcgaggatt cctttccggc ttcaccagcg gttgcggtgc
    12961 ggacataaac aacgctgggg atgccggatc gctgcatcaa tcgggcgtcc cgggtaaggg
    13021 ttacctgttg atcttcggga aagacatcgg aagggtcgta aaagtaggga agtgtctccg
    13081 gtggagggtc tggctgcgct tgtgctgcca tgacctctgg tacaccaggc agaaggaagt
    13141 agagcatcag cacggtgagt agggcggttc ccctcatcct ccagcagtga tggccatgcg
    13201 ttcgcactcc gcgaatgctc tctccactct cagcatccgc attacggtcg tgcagtgtcg
    13261 gctgcgggcg aatcattacg tgtaacaagt ttctggaccc cgtaggcggc gtatcccacg
    13321 ctgtagccga tggcgacaac catgccgagc aagataggaa gatagtagcg ttcaaagtct
    13381 actcgcatag tcatgatgat cgtcgccgtc tggaagataa cgagccccgc gatcgctagc
    13441 tccgcactgc ggagaccctt ccggaggccc acgccaagca ggagcaggat gccgatggtc
    13501 gccagcacaa cgtccagcat ggagaggcgg tctcccaatg ctggtaaacc gatggtggcg
    13561 agaaactctt cggtggagct ccatgcc
//
LOCUS       DASYWM010000275         3903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_50313, whole genome shotgun sequence.
ACCESSION   DASYWM010000275 DASYWM010000000
VERSION     DASYWM010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50313"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..2539)
                     /locus_tag="VGR29_11250"
     CDS             complement(278..2539)
                     /locus_tag="VGR29_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEV2074204.1"
                     /translation="MPGFEADHDTPRRVGQGFDETDAIRVLYGKDTSEGIVSIEPDGP
                     HHARVFRRDLASGETISHREPMPRWIVTTADHPSLGDMQGNYQQLAGDMPLATLVTFD
                     TRQTYSHAASLLQARDPDVIKVRSPLSQYLIASGQTFFKGMAFEDLRRMQLDIETLGF
                     DPTDPDAKIIMVAMRQGDWEDVLVLETDEADLLRQAAEHIRERDPDVIEGHNIFNFDL
                     PYLVERARRANVRLPIGRDGSDPNVADFTQRFRAGAISMPFTTVHIYGRHVIDTYQQI
                     QRWDTSGRLPSYGLKNVIRALSIEREDREFVEGEQIAILWKRGERSKERLARYALDDV
                     RDVDLLSRITLPTEFYQTQVLPMAFQTASTAGTGTKIDHLMMRAYLAELHSLPQPQEV
                     RPYPGGYVELLETGIFGPVVKCDVESLYPSIMLERRILPRTDVMGAFPLLLDDLTRRR
                     IESKRKAATSNGEEHALHDGLQATFKILINSFYGYLGFQRGHFNDYAAAEAITIEGQR
                     IIKEVVANLQQRGSEVIEVDTDGVFFTPPADVTTEEEEHALIRDVSRDMPLRIRLAHD
                     GRYQRMLSLKQKTYALIDFDGKLVMKGSALRSRRMEPCLQQFLQDATRHFLEDDKDAA
                     RERYLSLAQSIQERKLEPRDFSQWQMLREGTIDAHPRLRDLLKANPGRWRYGDRLEIY
                     ERQDGTLGFAEDYARDENVQVLLRHLRDAALRFEPIFESKAEFDAFFPAIQPTTDIAF
                     ARARRPVEQLSLL"
     gene            2551..3336
                     /locus_tag="VGR29_11255"
     CDS             2551..3336
                     /locus_tag="VGR29_11255"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV2074205.1"
                     /translation="MRERFPVMIATRDLRRLSVAARNILMDVLYPKTCAGCGMRGMWL
                     CELCSATLPLLLQHICDACGSPTHEPYGCRAIGPSVAKARAAYPYAGWAGAAIRRFKY
                     GNEPARAEHLAARMIPVLREFGAIDIVVPVPLHPKKLELRGYNQSELLARHIAAEMGL
                     PVRSLLIRNRATLPQVTLDQQRRHANVADAFGLHPGWAIEAGRNIMLIDDVRTTGATT
                     DACALVLKEEARAASVGVLTFAQELSQTDLERWTESLTGSPSP"
     gene            complement(3314..>3903)
                     /locus_tag="VGR29_11260"
     CDS             complement(3314..>3903)
                     /locus_tag="VGR29_11260"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEV2074206.1"
                     /translation="DALAHPNWSMGDKLTIDSATMMNKGLEVIEAHHLFNTAFEDIDV
                     VIHPEQIIHSMVELNDYSIIAQLSQPDMRLPIQYALTYPEHVHGPCRRLDFTTLRQLT
                     FEQPDLERFPALRLARDAGRAGQTFPTVLSAADEVAVNAFMEGRIRFLDIPEAVERVL
                     ERHDPIPVKDLETIAGVDRWARQAANDVVTAKVNR"
BASE COUNT          746 a         1166 c         1165 g          826 t
ORIGIN      
        1 cccgctctgc gtcccccaat ggtacccttg gggatggtct gggcaggatg agtagcaatg
       61 tcacctccgg tgacagcaca tccttcaggt accacagaag tgcgatccgt aacccacgac
      121 catgagcact cttccgccgg aagcaacgtc accctgaatg atgctgcaga ttccgacacg
      181 atagccctgg cagcttaccc catggacgga ttaccgcagt tgttcaagta ccccaaggta
      241 gctctaccta cgctcctcta gacgtttcat tctgatctca cagcaatgag agctgttcca
      301 ctggtctgcg tgctcgcgcg aacgcaatat cggtcgtggg ttggatcgcg gggaagaacg
      361 cgtcgaactc cgcttttgat tcgaagatgg gttcgaagcg gagtgccgcg tctcgcaagt
      421 gcctgagcag tacctggacg ttttcgtcgc gagcgtagtc ttccgcgaaa cctagtgttc
      481 catcctggcg ctcataaatc tcgagccggt ctccatacct ccatcgtccg ggattcgcct
      541 tcagcaagtc gcggagccgg ggatgtgcat cgattgttcc ttcccgcagc atctgccact
      601 ggctgaagtc gcgcggctcg agctttctct cctggatcga ctgagcaagc gaaaggtaac
      661 gctcgcgggc tgcatccttg tcgtcctcaa ggaagtgtcg ggtggcgtcc tgaagaaact
      721 gttgcaggca cggctccatt cgccggcttc gtagtgcact ccccttcatc accagtttgc
      781 cgtcgaaatc gatcaacgca tacgtcttct gcttgagaga cagcatccgc tgatagcgtc
      841 cgtcatgcgc gagacgaatc cggagcggca tgtcccgcga cacgtcccga atgagcgcat
      901 gttcctcttc ctcagttgtc acatctgcgg gaggggtgaa aaacacgccg tcggtgtcga
      961 cctcgatgac ctcggagccg cgttgctgga gattggcaac cacctcctta atgatgcgct
     1021 gtccctcgat agtaattgcc tcggcagcgg cgtagtcatt gaagtgtcca cgctggaatc
     1081 ccagataccc atagaaggaa ttgatcagga tcttgaatgt ggcctgaagc ccatcatgca
     1141 atgcgtgctc ttcaccgttc gaggtcgcgg ctttgcgctt cgactcgatc cgtctccgcg
     1201 tcagatcgtc gagcagaagc ggaaatgcac ccatgacatc ggtgcgggga aggatcctcc
     1261 gctccagcat gatcgatgga tacaggctct cgacatcgca cttgacgact ggtccaaaaa
     1321 tgccggtctc caggagttcc acatagcctc cgggatacgg tctgacctcc tggggctgag
     1381 gcaggctgtg gagttcggcc aggtaggctc gcatcattaa atgatcgatc ttcgtgccgg
     1441 ttcctgccgt cgaggcggtc tgaaaggcca tcgggagaac ctgtgtctga tagaactccg
     1501 taggaagggt gatccgcgag agcaagtcga catctcggac atcgtccagc gcatagcggg
     1561 caagccgctc cttgcttcgc tcacctcgtt tccagaggat ggcaatctgc tctccctcga
     1621 cgaattcccg atcctcgcgc tcgatgctca gggcccggat cacgttcttc aggccataac
     1681 tcgggaggcg gccggacgta tcccagcgct gaatctgctg gtacgtatcg atcacatgac
     1741 gcccgtagat gtgtaccgtc gtgaacggca tggagatcgc cccggcgcga aaccgttgcg
     1801 tgaaatcagc aacgttggga tcgctgccat ctcgtccaat cggaagccgc acgttcgcac
     1861 ggcgggcccg ctcaactagg tacggaagat cgaagttgaa gatgttgtgt ccctcgatga
     1921 cgtcggggtc acgttcccgg atgtgctcgg cggcttgtcg gagcaggtcc gcttcatcgg
     1981 tttcgagaac gaggacatct tcccagtctc cctggcgcat tgccaccatg atgatctttg
     2041 cgtcaggatc ggttgggtca aaaccgagcg tctcgatgtc cagctgcata cggcggagat
     2101 cttcaaacgc catccctttg aagaacgttt gcccggacgc gatgaggtac tgagacaggg
     2161 gcgaccggac cttgataacg tccggatccc gagcttgcag caatgacgcc gcatgcgagt
     2221 acgtctgcct ggtatcgaag gtcaccagcg tggccagtgg catgtcaccc gccagttgct
     2281 ggtaattccc ctgcatgtcg ccaagggaag ggtggtctgc cgtcgtcacg atccagcgtg
     2341 gcattggctc gcgatgcgag atcgtctcac ccgaagccag gtcgcggcga aagacgcgag
     2401 catgatgagg cccatcgggc tctatgctaa cgatgccttc gctcgtatcc ttgccgtaga
     2461 gcacccggat ggcatctgtc tcatcgaacc cctgcccgac cctgcggggc gtgtcgtggt
     2521 cagcttcgaa acctggcatg ttacggcctc atgagggaac gattcccggt gatgatcgcg
     2581 acgcgtgacc tgcgccgcct ctcggtggcg gcacggaaca tcctgatgga cgtgttgtac
     2641 cccaaaacct gcgctggatg cgggatgcgg ggaatgtggc tctgcgagct gtgcagcgcc
     2701 accctgccgc tgctcctcca gcatatctgc gacgcctgcg gatcaccaac gcacgaaccg
     2761 tatggatgtc gcgcgatagg gccttctgtc gcgaaggcga gggctgcata tccctacgcg
     2821 ggatgggccg gcgccgctat caggcggttc aagtatggaa acgagccggc gcgggccgaa
     2881 cacctggccg cgcgaatgat accggtgctc cgggagttcg gtgcgataga catcgtcgtg
     2941 cccgtgcccc ttcatccgaa gaagctcgaa ctccgtgggt acaatcagtc cgagttgttg
     3001 gcgaggcata tcgccgcgga gatgggactc ccagtgcgct ccctcttgat cagaaatcgg
     3061 gccacgttgc cccaggtcac gctggaccag caacgccgac atgccaacgt cgcggatgcc
     3121 ttcgggctcc accccggctg ggcgatcgaa gccggtcgaa acatcatgct gatcgatgac
     3181 gtgcggacca cgggagcgac aactgacgca tgtgcgcttg tgctcaagga agaggcgagg
     3241 gctgcgtccg tcggcgtgct gacgtttgcc caggagttgt cccagaccga tcttgagcgg
     3301 tggacagagt cccttaccgg ttcaccttcg ccgtaacgac gtcgttcgct gcctgccttg
     3361 cccacctgtc taccccggcg atggtttcca aatccttgac ggggatcggg tcatggcgtt
     3421 cgaggacccg ttcgacggct tccgggatgt caagaaagcg gatcctgcct tccatgaatg
     3481 cattgaccgc cacttcgtcc gcggcgctca ggaccgtggg aaaggtttgc ccggctctcc
     3541 ccgcgtcccg ggctagccgg agcgcaggga accgttcgag atcgggttgc tcgaacgtca
     3601 gttgccgcaa cgtcgtgaag tcgagtcgac ggcacgggcc atggacgtgc tcgggatacg
     3661 tcagcgcgta ctgaatcggc agtcgcatgt ctggctggct gagctgggcg ataatgctgt
     3721 agtcgttaag ctcaaccatg gagtggatga tctgttctgg gtgaatcacc acgtcgatat
     3781 cctcgaacgc agtgttgaac aggtgatggg cctcgatcac ttccaggccc ttgttcatca
     3841 tcgtggccga gtcaatcgtg agcttgtcgc ccatgctcca gttgggatgg gccagcgcgt
     3901 cct
//
LOCUS       DASYWM010000276         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_426089, whole genome shotgun sequence.
ACCESSION   DASYWM010000276 DASYWM010000000
VERSION     DASYWM010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426089"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VGR29_11265"
     CDS             <1..205
                     /locus_tag="VGR29_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074207.1"
                     /translation="LSGMSEVAEGVDTTRAAVELAHRTGVEMPISEQMYKVLFEGKSA
                     AEAVRDLMSREPKEEIHGRIESF"
     gene            complement(298..1656)
                     /locus_tag="VGR29_11270"
     CDS             complement(298..1656)
                     /locus_tag="VGR29_11270"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV2074208.1"
                     /translation="MVTTRGAVAGATRPLACLYSSPEKGHIMPATVIMGGQWGDEGKG
                     KLTDALASSAAMVVRANGGSNAGHTVKTDQGTFKLHLVPSGILNGNVICVIGAGVVIE
                     LSTLIGELDGLADRGVDTSNLRISDRAHLVLPYHPEIDRLEEARRESDLIGTTLRGNG
                     PAYADKVSRHGLRVCDLLDPSILRRKLAIEVDAKNEIITRVYESDRIDFDDLYQKLTQ
                     SAERLIPFIAPTEVLVQDTLHANNDVIVECAQGAMLDIDYGTYPFVTSSSPTAAGACQ
                     GAGVAPTQVERVIGVYKAYSTRVGAGPMPTELNDETGERIRRRGNEYGTTTGRPRRTG
                     WFDGVAARQTSRLSGVTELAVTLLDVLDMFDEIRFCSGYRLAGSDLTHVPASLERLEE
                     ATATWETLPGWNRDTTGARHYGDLPENAQAYLARIEEQLGAPARYIGVGPAREQLIDR
                     LV"
     gene            complement(1743..2831)
                     /locus_tag="VGR29_11275"
     CDS             complement(1743..2831)
                     /locus_tag="VGR29_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEV2074209.1"
                     /translation="MPVQFKDYYNVLGVERTATDSTIKTAFRKKARELHPDVNRDKPN
                     AEEQFKELNEAYEVLSDAEKRRMYDRFGEDWQRYRDAGINPDDVNASYGGGASQQSYQ
                     DFEQWFTGGGQSAPNGPWEWSERGGYRETSGRFSDFFNLLFGNEAPNAGARDRFNRPR
                     PIRGDDIEVAAEITLKEAAEGTARKLTLQTPASCQTCGGTGVARGAECPTCDGTGQVT
                     KSKTLEVKIPKGVKTGSRVRIAGQGGLGSYGGPNGDVYLMISVRDDPNFERIGDDIKT
                     TVRVPLFTAVLGGETVVPTLDGRVALTIPPGTQNEKVFRLRGKGLPKSGKGAAGQGDL
                     LATVHVDLPTDLSEEETKLFKQLRNLRQ"
     gene            complement(2892..3500)
                     /locus_tag="VGR29_11280"
     CDS             complement(2892..3500)
                     /locus_tag="VGR29_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEV2074210.1"
                     /translation="MLLSTLLAGFGLSASAGLNAYLPLLILALADRFTTTVELDQPYD
                     LLSSNIGILVIMLILPIELILDKIARVDHVNDLVHSVIRPITAAVAFMAFASQDRDIN
                     AVGAMIIGLVIGGAVHWLKAGTRPRISARTKGVGNPFISMIEDMLAIALSILAVFLPI
                     AIVAALPFGAWLLTRSYRRMSSGESRLMSLLGNQQPPAGSAT"
BASE COUNT          677 a         1049 c         1082 g          724 t
ORIGIN      
        1 gctgagcggg atgtccgaag tggccgaagg tgtagacacg acacgagcgg cggtcgagtt
       61 ggcgcatcgt accggcgtgg agatgccgat cagcgagcaa atgtataagg tcctctttga
      121 agggaaatca gccgccgaag cagtgcggga cctcatgtcc cgcgagccca aggaggaaat
      181 acacggccgt atcgaatcct tctagagggt cggcgaacgg cccgccgggg gaaagcgaca
      241 gtcgagcgtt cagtctgaga atcaggctga tcggcgtagt gtggtgcgat cctggtatta
      301 taccagcctg tcgatcagtt gttctcgagc cggccccaca cctatatagc gcgctggtgc
      361 cccgagttgc tcttcgatcc ttgcgagata agcctgtgca ttctccggga gatcgccgta
      421 gtggcgggcg cccgtggtat ccctgttcca gcccggaagt gtctcccatg tcgctgttgc
      481 ttcttccaga cgttcgagcg aagcggggac atgggtaaga tcgctgccag cgagcctgta
      541 accggaacag aatcggatct cgtcgaacat gtccagaaca tccagcaagg tgacggccag
      601 ctccgtgact ccactcagcc tgcttgtctg gcgtgcagca actccgtcga accacccggt
      661 acggcgaggg cgtccagtgg tggtgccgta ttcattacct cgccgccgaa tgcgctcccc
      721 ggtttcatcg ttgagctcgg tcggcattgg tcctgcaccg acacgggtgc tgtaggcctt
      781 gtatacccca atcacgcgtt caacctgtgt cggggcaacg ccggcgccct ggcacgctcc
      841 cgctgccgtt ggggatgacg aggtgacgaa cgggtaggtt ccgtagtcga tatcaagcat
      901 cgctccctgg gcgcattcga cgattacgtc gttgttggcg tgcagagtgt cctgcacgag
      961 gacttccgtg ggcgcgatga acggaatgag cctctcagcc gactgcgtga gcttctggta
     1021 gagatcatcg aagtcaattc gatccgattc gtagaccctg gtgattatct cgttcttggc
     1081 gtcgacctcg atcgccagct tacggcgcag gatcgaagga tcgagaagat cacagacgcg
     1141 caggccgtgg cgggatacct tatcggcata ggcgggtcca ttgccgcgca gggtggtgcc
     1201 gatgagatcc gactcccgcc gtgcttcctc gagacgatcg atctccggat gataggggag
     1261 gacaagatga gcgcggtcgg agatacgcag gttggacgtg tctactcccc ggtccgcgag
     1321 gccgtcgagc tcgccgatca acgtgctgag ctcgatgact acgccggcgc cgatgacaca
     1381 tatgacattg ccgttgagaa tgccggaggg aacaaggtgc aacttgaagg tgccctggtc
     1441 ggtcttgacg gtatgcccgg cgttgctacc gccattcgcg cgcaccacca ttgccgcact
     1501 ggaggccagc gcatcggtta gcttcccttt gccctcgtct ccccattgtc cacccatgat
     1561 gacggtcgcc ggcataatgt gccctttctc cggtgatgag tagagacacg ccaatggccg
     1621 ggtcgccccg gccactgcgc ctcttgttgt taccaatgtt ccaggtctcc ctggccgttt
     1681 gggcgcggac gcttatgtga cccgcgcgcg ccctggcgct cgccaccttt gcccacactg
     1741 gtctattgtc gcagattcct gagctgcttg aagagtttcg tttcttcctc gctgagatcg
     1801 gtcggcaggt ccacatggac ggttgccagg agatcgccct gtcctgcagc gcccttgccg
     1861 gacttgggaa gtcccttgcc cctgagccgg aaaactttct cgttctgggt tccagggggt
     1921 atggtcagcg cgacgcgtcc atccagggtc ggcacgacgg tttcgccgcc gagaacagca
     1981 gtaaagagag gaacgcgaac agttgtcttg atgtcgtcgc caatgcgttc gaagttggga
     2041 tcgtcgcgca ccgaaatcat gaggtagaca tctccgtttg gcccgccgta gctccccagg
     2101 cctccctggc cggcgatgcg gacacgggat cccgtcttga ctcccttggg gatcttcacc
     2161 tcaagggtct tactcttcgt gacctggccc gtgccgtcac acgtcgggca ctccgctcct
     2221 cgggcaaccc cagtgccgcc gcaggtctgg caggatgcag gagtttggag cgtcagcttg
     2281 cgagcggtgc cctccgctgc ctccttgagc gtaatctccg ccgcgacctc gatgtcatcg
     2341 ccacggatcg gacgcggccg gttgaaccgg tccctggcgc ccgcatttgg cgcctcgttc
     2401 ccgaagagga gattaaagaa gtcggagaat cggccgctcg tttcccggta gccgccgcgc
     2461 tcactccact cccacggccc gttcggagca gattgaccac cgccggtgaa ccattgctcg
     2521 aagtcctggt aggactgctg tgacgccccg ccgccgtagc tcgcattgac gtcatccgga
     2581 ttgatcccgg catcgcggta gcgttgccaa tcctcaccga agcggtcgta catccgtcgc
     2641 ttctcggcgt ccgagagaac ctcgtaggcc tcgttgagct ccttgaattg ctcttcggcg
     2701 tttggcttgt ccctgtttac atcaggatgg agctcccgcg ctttctttcg aaaggcggtt
     2761 ttgattgtgg agtcggtggc agtgcgctca acacccagta cgttgtagta atccttgaac
     2821 tgaacaggca tatctcaccc cagtactatg cactgggtat agtgtaaacc ttgagtcgat
     2881 acgacgcaac cctacgtggc cgagccggcc gggggttgct ggttgccaag aagggacatc
     2941 agccgggact cgccgctgct catccgccga taggagcggg tcagcagcca tgccccgaac
     3001 ggaagggcgg ccacaatcgc gatcggtagg aaaacagcaa gaatgctcag ggcgatcgcg
     3061 agcatgtctt caatcatact aatgaaaggg ttgccgaccc ctttcgtcct ggccgaaatc
     3121 cggggtcgcg tgccggcctt cagccagtga actgctccac cgatcacgag accgatgatc
     3181 atcgcaccga cggcattgat atctcggtcc tggctcgcaa aggccatgaa ggcaacggcg
     3241 gctgtgatgg gacggatcac cgaatgaacg agatcgttca catggtcgac gcgcgcgatc
     3301 ttgtcgagaa tcagctcgat cggcaggata agcatgatga cgaggatgcc aatattggag
     3361 gagaggaggt catacggctg gtccagctcc acggtggtcg tgaatcgatc agccagcgcg
     3421 aggatcagca gcggaagata ggcgttaaga ccagcacttg cagagagacc gaagccggca
     3481 agcagggtag aaagcaacaa gacacctcag tggggcgata aaggaacgca ct
//
LOCUS       DASYWM010000277         9599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_651170, whole genome shotgun sequence.
ACCESSION   DASYWM010000277 DASYWM010000000
VERSION     DASYWM010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651170"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..872
                     /locus_tag="VGR29_11285"
     CDS             306..872
                     /locus_tag="VGR29_11285"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEV2074211.1"
                     /translation="MNNISMDDLFYSFGIAHPGAVILHNFPRGLQFYEREDGIIQDLA
                     ATDILRSRELGVPRYNEFRRVLHLKPIERFEDLTDNPKWREELRCVYEDDVELLDLTV
                     GMYAEKFPAGFGFSDTAFRIFVIMASRRLNSDRFFTTHFNADVYSQAGLDWIADNDMS
                     SVLKRHYPALAPALDGVKNAFAPWSRVA"
     gene            1036..1638
                     /locus_tag="VGR29_11290"
     CDS             1036..1638
                     /locus_tag="VGR29_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV2074212.1"
                     /translation="MSGIPVRGQARGSADAAFAAARKRGCQRLDFTRDYFTALYRDAA
                     TLGIDADVLVGQWSLETGDGTSTYWIEDGNPGGLAAFDDGSNWGLTFSPEKAARAHAV
                     HMARYLGSTNVPDDWIATDARWQAVADAGYVGTVTTTDDLGNGRWATDPQYASKLRSR
                     YVAYWGEPGTTPVQPAGGTDVAITFGNVPIFGHTDRYISD"
     gene            1663..2577
                     /locus_tag="VGR29_11295"
     CDS             1663..2577
                     /locus_tag="VGR29_11295"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV2074213.1"
                     /translation="MGKRNPKFITLHRMVGSLTGTDGWFRRSDVSSITDYGVGIQAVD
                     GAKAGHIYRWNDPTGHRSGWSSGPVSAPFGDGALIVAKYGINAVNRDGISLEISGTDE
                     PIDDFSWGEIVHFCAWWIDRMKIPYTALPKNPHTGINVLIWHTEFTAGTGKQCPFPWM
                     RSKTEQLYTDVAAFLKPYQEGEKTDTGTTPAPDPIVEPVPEPEYVSPVPVLQLSTLGQ
                     QDADTAKSLVVEGATNFIFVNDRVRAIRNTPRYQRAIINGPRIGPDIKEGEEFEVLWH
                     FVEEDGTPWYLTPWWSRVLANDTQRIQD"
     gene            2902..3102
                     /locus_tag="VGR29_11300"
     CDS             2902..3102
                     /locus_tag="VGR29_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074214.1"
                     /translation="MRFRFCVSGQQHERLVLVYEKALAIIRSAMDSHTFADAWQRGGD
                     LTVQKALALLEGRMPFGGSPPV"
     gene            3374..4912
                     /locus_tag="VGR29_11305"
     CDS             3374..4912
                     /locus_tag="VGR29_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2074215.1"
                     /translation="MSDPRGGVVLEAKCITKTYGRTTANDCLTLSIRPGEIVGVLGEN
                     GAGKSTLLAILAGAIQPDSGQLLLNGKSTAFGSPRDALRAGVSIVFQHFALIHTFTVC
                     EQMHLAGWRSRELPDLLGSRFSGNELIGDISLGSRQMVEIARALVSRPRVLLLDEPTS
                     ILTSAETQQLFVLLRGLKEQGMSVVFVTHKLHEAMEVCDRIVVLRKGKDVGELEWSRP
                     GWPSGTDAGLLRLMFGRDEDGTDDAAPQLDTPELQVARSRDLPKRETGSLFCVQEVST
                     RDHPGSQALHRVSLDVGEGEICAIVGVDGQGQRELATVCAGYAPADGTVYLQWRALPI
                     GDAGAFRRAGIAYLTDDRMGEGTVPGFTLEENLILKRQREWPFSRRGRLRPGRVRDRA
                     REEVSRWRIEPQNPQAPIRTLSGGNIQKVLLARELSIASSLLIANNPSQGLDSRTRTQ
                     VWGAIKEFTDGQGGVLLFTTDINEALERADRVGVMYEGAVSPLTPVGDGIRSDLEHMM
                     VAGW"
     gene            4906..6000
                     /locus_tag="VGR29_11310"
     CDS             4906..6000
                     /locus_tag="VGR29_11310"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074216.1"
                     /translation="MVTAHLGFKETGWGRPRLILTSKWMDPLWAVLFALTCGAVLLIV
                     TGHDPVLAYREWVERAILRPSGIQETFVRAIPLLFTGMAVLLALRAGVWNIGIDGQVL
                     VGALAAAVTASSLVDMSAVVMWAGAAVAALLAGATWASIPALLRGRLGINEIITGIMF
                     NYIAMSMTSWLVKGPLGDPAVVLPQTKLIPVEMRLSTLGGTRVHIGLLLAVALVGCLG
                     WFLTRTVAGYELRATGASPAAAAHGQIPVAGYIIAALVASGAIAALAGANDVLSTKGA
                     FQGEWNPAYGFVAFALVFLGQRSVVGLVPAALVFGQLSYAADVMPRAADVAPAFFGVI
                     EGALLVALAVTVWARSTSVGRSGFGRGVKA"
     gene            5997..6941
                     /locus_tag="VGR29_11315"
     CDS             5997..6941
                     /locus_tag="VGR29_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074217.1"
                     /translation="MNIEAVITVLLSVALLSAVPLALASVGEAVGERAGLLNLGTEGI
                     MLLGAFTGFWVSLESRSLALGLLAGALTGVVSGLLFGVIAVVVGADQVLLGLGITLGG
                     SGLTSFLFRETFGASQPLLPGSMARPPGDLADALPIVGPALLGQPWFFYLAWVIVAGA
                     ELMLRGTTLGLRIRAVGESPIAVDTVGISVVSTRMFAAIIAGALSGLAGASLSIVELG
                     FFQPNVTLGTGFIAIALAMLGRWSPWRIAAMAIVFGSLRGLGSGLQLIDVSVQSEFTR
                     MLPYVGVVAALLLSGRGMRAPSALGQTWVRRRGAVRVR"
     gene            7071..8120
                     /locus_tag="VGR29_11320"
     CDS             7071..8120
                     /locus_tag="VGR29_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV2074218.1"
                     /translation="MKRTAFQLLMILTMLLPVGTSVAAEQEVELVSIVTPASRTNQAW
                     DQQGVDNLVATGEKMGFDVAVAENAGYEDITPILQDLADDGSDLIICHASGYQTVCPE
                     FAAQSGIPVVVIENPSAVTEGLVSDIETQAQEAAYLAGVLAGLETKTGTIAIVVTGEP
                     PTWNYMTVGFAEGLHSVRPDATLLYSIVGEAAYDDAPNAKRVTEAVLAGGADIVFGMG
                     DGASFGMIQAIREYNAEQPDKQQARFIDVIGDKSGSEAKDFLLTSVLFDYKGVYKDLV
                     SDLKAEEFGSVVTMNLENNGVRLLEPPIRVDPEHLQILSETRNKIVDGEIEVSAIGDA
                     DGTHERLEELFPGDN"
     gene            complement(8277..9020)
                     /locus_tag="VGR29_11325"
     CDS             complement(8277..9020)
                     /locus_tag="VGR29_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074219.1"
                     /translation="MHSRAHEPILLQLGEERWEQFSGDIAKWLRNVQLAQSSFRHLAE
                     KTATGILDPRVKEMIERIAGSAKDHEKRVDELYRLICREPSNITRMLGEVTARGRQML
                     GNVLGMTGGSSGPWHDLRQMLLANLNAIGAFGAAEQLGLTIGHPQLAHETFSIVRDKS
                     TQQLVIEEYMLELAPQAILYHQEEQGSGEAVDDHHRYPPDEPEGTQPGLPLPGRPDNL
                     VVGNQMGGDLQDATQQQHESSDYGGGNEM"
     gene            complement(9031..>9599)
                     /locus_tag="VGR29_11330"
     CDS             complement(9031..>9599)
                     /locus_tag="VGR29_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074220.1"
                     /translation="IWHIRNASQPYGSIETPDWYGFPDICKDGLPAWDKKHLPNRGIP
                     AEPLIEDPPPWAGPAAFLEKPHSSMAKMDFSPSDAFGFRGELFACEWGTMAPLNTNRT
                     EALDRGFKVIRLNTTTGQAETFLQNKQPAPASYWTGSGGIERPVSCRFSPDGSELYVL
                     DFGVAAVNENAMVAYGHTGVLWRITRAQ"
BASE COUNT         1864 a         2775 c         2988 g         1972 t
ORIGIN      
        1 ctccagacaa tctgacgggc cgtttgtacg tacacactac aggacgtgtg cctccctgtc
       61 gataatccga gcgaggttgg tctgtgtgct tcgagccccc gtgcagttcg gcaatctcgc
      121 gagcgcgacg ggcacgacaa ccgccctcaa ggtctcagga gccgcgtcgg ttgctatcgc
      181 tacggggttg accccttcag cagagagagg caggacggga gacaacgccc cgcacatcga
      241 aaacggacgc actgctggaa aaacgacagt tccctcaagt cgatggcagg gaggccctcg
      301 aaataataaa caatatctcg atggacgacc tgttctattc cttcgggatt gcccatccag
      361 gggcagtcat ccttcacaat ttcccgcgag gtctgcagtt ctatgaacgg gaggatggga
      421 tcatccagga cctggcggcg actgacatcc tcaggagtcg tgagctgggc gtcccacgct
      481 acaacgaatt ccgacgcgtg ttgcacctga aacccatcga gcggttcgaa gacctcacgg
      541 acaatcccaa atggcgtgag gaactgcgct gcgtctacga agacgatgtc gagcttctcg
      601 atcttacagt cggaatgtac gcagagaaat tccccgctgg cttcggcttc agtgacaccg
      661 cattccggat cttcgtgatt atggcatcgc ggcggctcaa cagcgaccgt ttcttcacga
      721 cccatttcaa cgccgacgtt tacagccagg ctggacttga ctggattgcc gacaacgaca
      781 tgtccagcgt cctgaagcgt cattaccccg cactcgcccc ggcgctggat ggggtcaaga
      841 acgccttcgc gccgtggtcg agggtggcgt gagtccctgg atggccgacg tctgcagcag
      901 gggcgcggca tcgatcacag tgtttttccg gcatggaggt acaggaggag gtggcacccg
      961 tgcgaggacc gctgtctacg tggatcgtct ggatttcgtg atttctacgg agtccggagt
     1021 agggaggaat acaccatgag cggcataccg gtcagggggc aagccagggg ctcggccgat
     1081 gctgcgttcg cggccgccag gaagcgcggc tgccagcggc tcgatttcac gcgggactat
     1141 ttcacagcgc tctatcggga cgcggcgaca ctgggcatcg acgccgatgt ccttgtaggg
     1201 caatggtctc tcgagaccgg cgacggcaca tcgacgtatt ggattgagga cggcaatccc
     1261 ggcggtcttg ccgccttcga tgatggttcc aactggggcc tgaccttctc gccggagaag
     1321 gctgcacgcg cgcatgctgt tcacatggcg cgatacctcg gctcgacaaa cgttcccgac
     1381 gactggattg ccactgatgc ccggtggcag gctgtcgcgg acgcgggata cgtcggcacc
     1441 gtcacgacga ccgacgacct cggcaacggg cggtgggcga ccgatccgca gtatgcatcg
     1501 aaactccggt cgcgctatgt ggcgtactgg ggtgaaccag gcacgacgcc ggtccagccg
     1561 gcaggaggta cagacgtggc tattacattt ggcaatgtcc cgatcttcgg ccacaccgat
     1621 cgctacatct cggattagcg tgaagggttt ggctgggaca atctgggcaa gcgcaacccg
     1681 aagttcatca cactgcaccg catggtgggc tcgctgacag gcaccgacgg ctggtttaga
     1741 cgatcagatg tctcgtccat caccgactac ggagtcggca tccaggcggt tgatggagcg
     1801 aaggcagggc atatttaccg ctggaacgac cccactggtc accgctcggg ttggtcctcg
     1861 ggtccagtat cagcaccgtt tggcgacgga gccctgatcg tcgccaaata tggcatcaac
     1921 gcggtcaatc gcgacggcat ctcgctggag atcagcggga cggacgagcc cattgacgat
     1981 ttctcctggg gcgagattgt tcacttctgc gcctggtgga ttgacaggat gaagatccct
     2041 tacacggcct tgccaaaaaa tccgcatacg ggaatcaacg tcctgatctg gcatacggaa
     2101 ttcaccgcag gaaccgggaa acagtgcccg tttccctgga tgcgaagcaa aaccgagcag
     2161 ctctacactg acgtggctgc gttcctgaag ccgtaccagg agggtgagaa gacggacacc
     2221 ggaacaactc cggcgcccga tcccatcgtt gagccagttc cggaaccaga gtatgtgtcg
     2281 cctgtccctg tacttcagct ttccacgctg ggtcaacagg atgctgatac cgcgaagtca
     2341 ctcgtagtgg aaggtgcgac gaacttcatc ttcgttaatg accgcgtgag agcgatccgg
     2401 aatacgcccc ggtaccaacg ggccatcatc aacggtccga ggattgggcc ggatatcaag
     2461 gagggtgagg agttcgaagt gctttggcac tttgtcgaag aggatggcac gccctggtac
     2521 ctcacaccct ggtggagccg tgtcctggcc aatgacaccc agcgtatcca ggactaagag
     2581 atcatcagga atggtggaac tgacgatcac gctccgttgc agcccggtag gtcgagtacc
     2641 gcggcgactc gatcgaagtg acccgacgga gcgcgatcgt tgggctcaga cgctgagtgg
     2701 gatgaatagc aacaccgccg acagcctcgc ggtgttgacg tggcgactcc tctggaggtt
     2761 ccggcgattt catttcccgt tgaagagtaa ctccagatgt acaccagtgc attgcgaata
     2821 aagcttgagc cacatgaacc cgaagcagca ttggattgct gaccacccat cacatccatc
     2881 aggaagacgt ccgctgaagc gatgcgattt cggttctgtg tctcaggaca acaacacgaa
     2941 cggctggtcc tggtctatga gaaggcactg gcgatcattc gttccgcaat ggactcacac
     3001 acattcgccg atgcctggca aagaggtgga gacctgaccg tgcagaaggc gcttgccctg
     3061 ttagaggggc gcatgccgtt cggcggttca cccccagtgt agcgccggta ctcctactct
     3121 cgtcccatac ggtcgattac cgaaccccca tatgtccgtc ccgacaactg acaaagccag
     3181 caggaacgtc ctttctacag gatcggtcgc tgcccggtcc ggatggcata atccgttgga
     3241 gtgcgctcaa ctacctgccg cgctgtgccg cagggcagct acgtgtggcg aggctcttgg
     3301 gcagtctgta cagcctcctc ccagggaact gcgacagcct gatcgattac agacagcatc
     3361 ctatctgagg tttatgtcag acccccgagg cggagtggtg ctcgaagcca aatgcattac
     3421 caagacatac ggacgcacaa cggcgaacga ttgcctgacc ttgtcgatcc gcccgggcga
     3481 gatcgtaggc gtccttggag aaaacggcgc cggtaagtcg acgcttctcg cgattcttgc
     3541 gggtgccatc cagcccgata gcggtcagct cctccttaac ggaaagtcca cagcatttgg
     3601 ttcaccaaga gacgccctcc gggccggagt gtcgatcgtg tttcagcact tcgcgctgat
     3661 tcataccttc accgtttgcg agcagatgca cctggctgga tggagatcca gggaactccc
     3721 ggatcttctc gggtcgcgct tctccggaaa tgagctgatc ggcgacatat ccctcggcag
     3781 tcgacaaatg gtcgaaattg ccagagcgct ggtctctcgt ccacgcgttc tccttctgga
     3841 cgagccaacg tccatcctca cttcggctga gacgcaacag ctctttgtgc tcctgcgcgg
     3901 cctgaaggag caaggaatgt cggtcgtgtt cgtcactcac aaattgcatg aggcaatgga
     3961 ggtgtgcgac cgcatcgtcg ttctccgaaa aggcaaggac gtaggtgaac tggagtggtc
     4021 acggcccggc tggccatccg gaaccgacgc gggcctgttg aggctgatgt tcggtcgaga
     4081 tgaggatggt accgacgacg ctgcgccgca actggacact ccggagctac aggttgcccg
     4141 atctcgtgac ttaccgaagc gcgaaacagg ttcactgttt tgtgtgcagg aagtgtccac
     4201 tcgggatcac ccgggaagtc aggccctgca tcgggtttcg cttgatgttg gcgaggggga
     4261 gatatgcgca attgtcggcg tcgacgggca ggggcaacgc gaactggcga cggtgtgtgc
     4321 tggttatgca ccggccgacg gcaccgtata cctgcaatgg cgcgccttgc cgatcggtga
     4381 tgccggggcg ttcagacgag cgggaatcgc ctatctcact gacgatcgca tgggtgaggg
     4441 gaccgtcccg ggattcacgc ttgaagagaa cctcattttg aaacggcagc gcgagtggcc
     4501 cttcagccgt cgaggtcgat tgcgcccagg cagggtccgg gatcgggcca gggaagaggt
     4561 gtcgcggtgg cgtatcgagc cccagaatcc tcaggccccg attagaacac tttccggggg
     4621 aaatatacag aaggtccttc tggcaaggga gctctcgatc gcatcctcgt tactcatcgc
     4681 caacaatccg tcgcaggggc ttgatagcag aaccagaacg caggtctggg gcgcaatcaa
     4741 ggagttcacc gacggccaag ggggcgtact cctctttaca acagacatca atgaggcgct
     4801 cgagcgcgct gatcgggtag gggtgatgta cgagggcgct gtctcgccac ttacgccggt
     4861 aggtgacgga attcggtcag acctcgaaca tatgatggtc gccggatggt gacggcgcat
     4921 ctcggattca aggaaacggg ttggggccgc ccgcgactca tcttgacctc aaagtggatg
     4981 gatccattgt gggcagtgct gttcgcgctc acatgcgggg cggtcctcct cattgtcacc
     5041 ggtcatgacc cagtgctcgc ataccgggaa tgggtcgagc gcgccatcct tcggcccagc
     5101 ggcatccagg aaacattcgt cagggcaatt ccgcttctgt tcacgggaat ggctgttctg
     5161 ctcgccctgc gggcgggagt atggaacatc ggtatcgatg ggcaggtcct cgtcggtgca
     5221 cttgccgctg cggttaccgc cagctccctg gtcgatatga gcgccgtggt gatgtgggca
     5281 ggcgcagcag tggcagccct tctcgcgggt gcgacgtggg cttcaattcc ggcactgctg
     5341 cggggccggc tgggcatcaa cgaaatcatc accgggatca tgttcaacta catcgcgatg
     5401 tccatgacct catggctggt caagggacca ctgggcgatc ccgctgtggt gttgccccag
     5461 accaaactga tcccggtgga gatgcggttg tcgacactgg gcggcacccg tgtacacata
     5521 gggctcttgc tcgccgtcgc gcttgtcggt tgccttggct ggttcctcac gcgcacggtg
     5581 gccgggtacg aactgagggc gacaggtgcc agcccggccg ctgccgcaca tgggcagatc
     5641 cccgtagcgg gctacatcat cgctgctctg gtagccagcg gggcgatcgc ggccctcgcg
     5701 ggtgcaaatg acgtcctgtc taccaagggc gcattccagg gcgaatggaa tcccgcatac
     5761 ggctttgtgg cgtttgcatt ggtgttcctc ggtcagcgaa gtgtcgtcgg cctggttccg
     5821 gcggcactgg tgtttggtca gctttcctat gccgctgacg tcatgccccg cgccgctgac
     5881 gtcgctcccg ccttcttcgg cgttatcgag ggggctttgc tggtggcact tgccgtgaca
     5941 gtgtgggcaa gatcgaccag cgttggccgc tccgggttcg gacgcggggt caaggcatga
     6001 atattgaagc ggtgattacc gtgctcctct cggtcgcgct gctcagtgca gtaccactcg
     6061 cacttgcaag tgtcggcgaa gcggtgggag aacgggcagg tttattgaac ctcgggaccg
     6121 aagggatcat gcttctcggc gcgttcaccg ggttctgggt ctcgctggag agccgttcgc
     6181 tcgcgttagg tctccttgcc ggagctctca cgggagtcgt cagtggcctg cttttcggcg
     6241 tgatcgcggt ggtcgttgga gcagaccagg tcctgctcgg gcttggaata accctcggcg
     6301 gaagtgggtt gacctccttc ctctttcgcg agacgttcgg tgcctcgcag ccgttactcc
     6361 ctggctcaat ggcgaggcct ccaggcgacc tcgcagatgc actgcccatt gttggtccag
     6421 ccttgctggg ccagccatgg ttcttctacc ttgcctgggt catagtggcg ggggccgagc
     6481 tcatgctcag ggggacgacg cttggcctga gaatccgggc ggtcggtgag tctcccatcg
     6541 cggttgatac cgtgggaatc agcgtcgtgt cgacacgcat gttcgcggcg atcatagcgg
     6601 gtgcactatc ggggcttgca ggtgcatctc tcagcatcgt tgaactcggg tttttccagc
     6661 cgaacgtcac gctcgggacc gggttcatcg cgattgccct ggcaatgctt ggccggtggt
     6721 cgccttggcg aattgctgcg atggcgatcg tgtttggctc gctccgcggg ctcggaagcg
     6781 gcctccagct catcgatgtt tccgttcagt cggagttcac gaggatgctg ccatacgtcg
     6841 gggtcgtggc cgccttgctc ctgagcggac gcggaatgcg tgcgccgtcc gcgcttggcc
     6901 aaacgtgggt tcgaagacgg ggtgccgtac gcgttcggta atgacgtcct tgcattcgtg
     6961 gaaatgggcc ggcaaggagt gcaagttttt ccggggcaga ccgatcgaag tatgatgaga
     7021 cgagaagcac aagcgcctcg atatgccttg ccggagaagt gagacgacgt atgaagagaa
     7081 ccgcctttca actgcttatg atccttacca tgctcctgcc agtcggtact tcagtggccg
     7141 ctgagcagga agtggagctc gtgtcgattg tgacgcctgc gagtcgcacc aaccaggcgt
     7201 gggaccagca aggtgtcgac aatcttgtcg cgaccggcga gaagatgggc ttcgatgtgg
     7261 cagtagcgga gaacgcggga tacgaggaca tcactcccat tctccaggat ctcgcggatg
     7321 atggatcgga tctcatcatc tgccatgcgt ccggctatca aacggtatgc ccggagtttg
     7381 ccgcgcagtc gggtatccct gtggtggtca tcgagaaccc ctcagcggtg acggaaggtc
     7441 tggtatcgga tatcgaaacc caggcccagg aggcggcata tctggcgggg gtgctcgccg
     7501 gtctggagac gaagaccggc acgatagcaa tagtggtcac cggggaacct ccgacctgga
     7561 actatatgac cgtaggcttc gccgaaggac tccactcggt ccggccggat gccaccctgc
     7621 tctacagcat cgtcggagaa gcagcgtacg acgacgctcc aaatgccaag cgggtgactg
     7681 aagcggtgct tgcaggaggt gcggacattg tcttcggcat gggagacggc gcgtcattcg
     7741 gcatgatcca ggcgatccgt gagtacaacg ctgagcagcc ggacaagcag caggcccgct
     7801 tcatcgatgt gatcggcgac aagtccgggt ctgaggcaaa ggatttcctg ctgacgtcgg
     7861 tactcttcga ctacaaaggg gtgtacaagg atctggtcag cgatttgaaa gcggaagaat
     7921 tcggcagtgt cgtgacgatg aacctcgaga acaacggcgt tcgtctgctg gaaccaccaa
     7981 ttcgcgttga tcccgaacac ctgcagatac tgtccgagac acgcaacaag atcgtggacg
     8041 gcgagatcga ggtttctgca ataggcgatg cggacggcac ccacgaacgc ctggaggaac
     8101 tctttccagg ggacaactga tcgacgacgg gcgtggcgtc tacgagcctg ctgatagcag
     8161 cggaccagca atagggtctg ccgcctggag agtcggttcc acggcaaacg ggggaggtag
     8221 cgtgatatcc ttgcgcctcc gctggcggcc gacagggtgc tttcgcggac ctctccctac
     8281 atttcgttcc cacctccata gtcactgctc tcatgctgct gttgcgtggc gtcctggagg
     8341 tcgccaccca tctggttccc cacgaccagg ttgtccggtc tgccaggcag aggcaggccc
     8401 ggctgggtcc cttccggctc gtccggtgga tagcggtgat gatcgtcaac cgcctcaccg
     8461 gagccttgct cttcctgatg gtaaagaatg gcctgggggg ccagctcaag catatactcc
     8521 tcaatgacca gctgctgagt cgatttgtcg cgaacgatcg agaaagtctc gtgggcgagc
     8581 tgcggatggc caatcgtgag ccccagttgc tccgctgcgc caaacgctcc tatggcattg
     8641 agatttgcca gcagcatctg ccgcaggtca tgccatggac ctgacgaacc gccggtcatt
     8701 cccagcacat tgccaagcat ctgccgtccc ctggctgtta cctccccgag catcctggta
     8761 atgttggaag gttcacgaca gatcaggcgg tatagctcgt ccaccctctt ctcgtggtcc
     8821 ttcgcagacc cggcaatacg ctcgatcatc tccttcacac gggggtccag aatcccggta
     8881 gcagtcttct ctgccaggtg ccggaacgaa ctctgggcga gttggacgtt cctcaaccat
     8941 ttggcgatat cgccgctgaa ctgctcccag cgttcctcac caagttgaag caggattggc
     9001 tcatgggctc tgctgtgcat acggctctcc ttactgcgcc ctggtgatac gccacagcac
     9061 gccagtatgg ccgtacgcga ccatagcgtt ctcgttcacg gcggcaacac cgaaatcgag
     9121 gacatatagc tcgcttccat cggggctgaa cctgcacgag acaggacgct cgataccgcc
     9181 gcttccagtc cagtaggaag ccggcgctgg ctgcttgttc tgcaggaacg tttccgcctg
     9241 gcctgtcgtc gtattgaggc ggatgacctt gaagccacgg tccaatgctt cggtgcgatt
     9301 ggtgttcagt ggcgccatgg tgccccattc gcaggcaaag agttcgcccc tgaaaccgaa
     9361 ggcatcggat ggtgagaagt ccattttcgc cattgagcta tggggcttct ccaggaatgc
     9421 ggcaggccca gcccaagggg gtggatcctc gatcaggggc tcagccggga tgcctctatt
     9481 tggcagatgc ttcttgtccc acgcaggtag tccatccttg cagatgtccg ggaagccgta
     9541 ccaatcgggc gtctcgatgg agccataggg ctggctggca tttcggatgt gccagatgc
//
LOCUS       DASYWM010000278         5788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_500748, whole genome shotgun sequence.
ACCESSION   DASYWM010000278 DASYWM010000000
VERSION     DASYWM010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500748"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /gene="otsB"
                     /locus_tag="VGR29_11335"
     CDS             <1..463
                     /gene="otsB"
                     /locus_tag="VGR29_11335"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEV2074221.1"
                     /translation="LEAVTSTEGMLFENKRLSASIHYRNASAPVNIGLVLLPIVEEEA
                     AANGLRVSGGKMLVELRPTAVVSKGTALEEIVRTRNLKGAVFFGDDVTDVDGFRALHR
                     MRDEQGLKAAAVAIRSEDVHPDVVSESDVVLEGVPDMVVTLHRIADLMCST"
     gene            complement(590..1258)
                     /locus_tag="VGR29_11340"
     CDS             complement(590..1258)
                     /locus_tag="VGR29_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEV2074222.1"
                     /translation="MTSLNRANPSPSDGLIKAVGDVIRRFESMSAARDAAVTDGRQVV
                     RLSANAVRVLHRGEDASGLLASARTMMSSIAQKTDPYPNIYWAGYVQDAMKEYAEAEI
                     TAALLQGKRIPSAEDLEVEDASWLNGLAEAGSELRRDVLDALRRDDITRAETLLDEMD
                     AVYSVLVTVDYPDAVTGGLRRTTDQFRSVLERTRGDVTVSVRQSRLEQALRKSEVERA
                     DSTG"
     gene            complement(1468..3003)
                     /locus_tag="VGR29_11345"
     CDS             complement(1468..3003)
                     /locus_tag="VGR29_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074223.1"
                     /translation="MRHTYTRPVWLVIAAFAMMLGVLGAAAPAATAQDSQIPENAPVD
                     IHSGTCEDFLDEPAYDGGAITPTTLNEVRDDDTFGAGILEDEELGATGVDLNQDDALG
                     DDEVVGAIDEDAPVGFAENDLESDVNTDDPYVVAVHAGPDQYETILACGSVGGAQEVD
                     GNQVVRLQPVGDAQFFGYSVLGGDADSLTTYLFQPGTPPEAAAVQDIPGHPVDIHTGT
                     CSDWTEEPVFDVGDMLETNVAAPGEQEAGDMEGEIPAGAENLGPVFVVDNETEFNGTE
                     LAETGEPLVVAVHESAENYSTLVACGQILEIVEEDHIFVILQPVGDSNMTGFLQIFND
                     EEEAHGLLWSCAPLEPQPVEPTPTPEPTPTPTPEPTPTPEPTPTPEPSPTPTEAAVIE
                     ETEVVTEIATEVLTETEVVPAPTATALAQLEGDAADAAAIELGEEPAGPVTSQAGQTI
                     TVNNTSDVERVFRVEELGIEETIPAGEEVEIQLPEDAEAGTYPYQVLEGDEVVFEDDF
                     TIE"
     gene            3392..5245
                     /locus_tag="VGR29_11350"
     CDS             3392..5245
                     /locus_tag="VGR29_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074224.1"
                     /translation="MTSGADARLPMLDHRRDSRQVAATNHAVTLDPPRARVPGHAKSR
                     DHLIAIALGMIVGLGFMLRLFGATQMSPHVDEPASILAAHAAAERGLPILPSGTPYFQ
                     GATLSYLLVPFVWLGFGDLDNLALMRLVPVAAGTLAVFLGYGLGRYVTGNPWIGAAMA
                     ALVAIDPISVQWSGHVRMYALLQVLTVGLAWAFIHLLMRGPSKRRVATVVALFWLAVF
                     THVGATLLLPAMLLTVVVLTRRGLVPRNGFLTLFTLCGMAPLLLLLLNRMLGVTSGDL
                     GESSRGPAIPFVGDNLIAPLARFQVSPAEWELNALAKPSTLFWLIPGLIVAICSVITA
                     RRYLLNRNCAASAETRIGCTVCFIMYWLPTLTVALLTASPKPRYLLHVHVLGYLFVAV
                     LLSRLTRAHWCERAGRRNVVGSALACSAIVIVLAGIGGGLVWRLQHLVVHPDYHTAMA
                     YAASNHSPGQPVIVALPPVAYLAMDAADRDDLYFLAGSRQSTRAERYTLATEDGRIVD
                     YWVGAESVVTAQGLRSILLENPDSLLVIDEHRLTAEWAYGGKMEDVIREMTTVVHRGP
                     GGALVLTPSEGGKSTTRDRDLFFQSGGEQGPGAIGSPCMPARSSRVCGGQA"
     gene            5710..5782
                     /locus_tag="VGR29_11355"
     tRNA            5710..5782
                     /locus_tag="VGR29_11355"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5743..5745,aa:Ala,seq:ggc)
BASE COUNT         1078 a         1729 c         1756 g         1225 t
ORIGIN      
        1 gctcgaagca gtaacgtcca cggagggcat gctcttcgag aacaagcgtc tttcggcctc
       61 catccactat cgcaacgcat ccgccccggt gaatatcggc ctggtgctcc tgcctatcgt
      121 tgaggaagag gccgcagcga acggtctccg ggtctcgggc gggaaaatgc tggtggaact
      181 ccgtccgacc gcggtggtga gcaagggcac ggcgctcgaa gagattgtca ggacgcgcaa
      241 cctcaagggc gcggttttct ttggcgatga tgtgaccgat gttgacggtt tccgggcgct
      301 gcaccggatg cgagatgaac aagggttaaa ggcagcagca gttgccattc gttccgagga
      361 tgtgcatccc gacgttgtct ccgagtctga cgttgtgctc gagggcgtgc cagacatggt
      421 cgttacgctc caccggatcg cagacttgat gtgttctacc tgacagcagg cggcgttccg
      481 tgcgctaatg gcgtcgacgg gtgatcctca gccgcttggc tcttcccttt gctgggcgct
      541 ggtatagact cgagacacgt gcatggacac caatccactc cgacaattcc taccctgtgc
      601 tgtccgctct ttccacttct gacttgcgta gggcctgttc caggcggctt tgtcggacag
      661 agacggtcac atctccccgt gtgcgctcaa gcactgatcg gaactggtcg gtggtgcgcc
      721 gcaaaccgcc ggtcacggcg tccggatagt ccactgtcac gagcacgctg taaacagcgt
      781 ccatttcgtc caaaagcgtc tcagcccgag tgatgtcatc tcgtcgaagg gcgtcaagca
      841 catcccggcg tagctcgctt ccagcctctg ccagcccgtt gagccatgag gcgtcctcga
      901 cctccagatc ctcggcactc gggatcctct tcccctgaag cagtgcagcc gtgatctccg
      961 cctccgcata ttccttcatc gcgtcctgca cgtatccagc ccagtagatg ttggggtatg
     1021 gatcggtctt ctgggcaata gaagacatca tcgttcttgc actggcaagc agtcccgatg
     1081 cgtcctctcc acgatggagc acacgaaccg cgttagcgga cagccgcacc acctgccgtc
     1141 catcggttac tgctgcatcc cgcgccgcac tcattgattc gaagcgacga ataacgtctc
     1201 caacggcttt gatgagaccg tcggaaggac ttgggttcgc tcggttcagg gatgtcacag
     1261 ctgtattgcc tccggggaat gagagaaacc aggtcatcaa aagcctttcg tcggttgtag
     1321 agaacgcctc gaatgccaag cggcagaaaa atctaatcgt ggccaagtac tgaagaccat
     1381 gtgaggagga cgcaaactgg cgccggatca acgttgatcc ggcgccagag cgagcaatct
     1441 cgtaatggtc gggctatgcg ccagccgtta ctcgattgtg aaatcgtcct cgaagacaac
     1501 ctcgtcgcct tcaagcacct gatatgggta ggtaccggct tcggcatcct cgggcagttg
     1561 gatctcaact tcctcacctg ccgggatggt ttcctcgata cccagctcct cgacacggaa
     1621 tacgcgttca acgtccgagg tgttgttcac ggtgatcgtc tggccagctt gcgacgtcac
     1681 cggaccggcc ggctcttcgc cgagctcgat cgcagcagcg tcggctgcat caccttccag
     1741 ctgggccaac gccgtagcgg tcggggctgg aacaacttct gtctcggtca agacttcggt
     1801 cgcgatttcc gtaacaactt ccgtctcctc gattaccgcg gcctctgtcg gcgtcgggct
     1861 cggctccggc gtcggagttg gctccggcgt cggggttggc tcgggcgtcg gggtcggggt
     1921 cggctccggc gtcggggtcg gctccacagg ctggggctcg agtggcgcgc agctccagag
     1981 gagtccatgc gcctcttcct catcgttgaa gatctgaagg aatcccgtca tgttactgtc
     2041 gccaacaggc tggaggatca caaagatgtg gtcctcttcg acgatctcaa ggatctgccc
     2101 gcaggctacg agcgtggagt agttctcggc gctctcatgc acggcgacca ccaatggttc
     2161 gccggtctcg gcgagctcgg tgccgttgaa ttcggtttca ttgtcgacta cgaagaccgg
     2221 gccaaggttc tcggcgccgg cggggatttc gccctccatg tcgcctgctt cttgctcgcc
     2281 aggagcggca acattcgtct ccagcatgtc gccaacatcg aagactggct cttccgtcca
     2341 gtcagagcac gtgcctgtgt gaatatcaac gggatgaccc gggatgtcct gaacggcggc
     2401 ggcctccggc ggtgttccag gctggaagag gtaggtggta agggaatcgg catcgccccc
     2461 aaggaccgag tagccgaaga actgggcgtc gccgaccggc tggagacgga cgacctgatt
     2521 tccgtctacc tcttgcgcac cgcccactga cccacaggca aggatcgttt catactggtc
     2581 cgggccagcg tgcacggcaa caacatacgg atcatcagta ttgacgtccg actcgaggtc
     2641 attctcggcg aagccaaccg gggcgtcttc gtcaatggca ccgacaacct cgtcgtctcc
     2701 caaggcgtcg tcttgattga ggtcaacgcc tgtcgcgccc aactcctcgt cctcgaggat
     2761 accggcaccg aaggtgtcgt cgtcccggac ttcgttgagt gtcgttggag tgatggcgcc
     2821 gccgtcatac gccggctcgt cgaggaagtc ttcacatgta ccggagtgaa tatcgacggg
     2881 agcgttctcc ggtatctgcg aatcctgggc cgttgctgcc ggggcagcag cgcccagcac
     2941 gccgagcatc atcgcgaacg cggctattac cagccagact gggcgggtat atgtgtgtct
     3001 catcgtttct cctctattgg tcgtccctgt tgtgcaggaa cttcgtcgcc ggtatttcca
     3061 taccgttact gctcaaggcg ccgcggcgcg ctcccccttt ccataccgag cgaactgccc
     3121 tcacgaaaac tccagctggt cgatagtggt ccgaccgatg ttttttccgc tgcacgccct
     3181 gtgccatcgc ccacgtcaga aacgatattc cgggaacgta gttgcggaaa gcttttaggc
     3241 aatgtcacct tgcctggacc taatccatac accggtctgc tgagggtacc atactcgtgt
     3301 cagcacgtgg gtgcggcggc cccagctggc atataactgg cttaccgact gactcgtcag
     3361 ctcgaggagg tgacgaagga gtgatgcagg ggtgacgagt ggggctgatg cgcgtttgcc
     3421 gatgcttgat caccggcggg acagtcggca agttgcagcg acaaatcacg ctgttacgct
     3481 cgatcctccc cgcgcaagag tacccgggca tgcaaagagc agggaccacc ttatcgccat
     3541 tgcgttaggg atgatcgtgg gcctgggctt catgctgagg ctgtttggtg caacccagat
     3601 gtcgccccac gtagatgagc cggcgagcat attggcagcc cacgccgcgg ccgagcgagg
     3661 tctgccgatc ctgccttccg gaaccccgta cttccaggga gcaaccctct cctatctcct
     3721 ggtgccgttc gtgtggctag gcttcgggga tctggacaat cttgcgctca tgcgactcgt
     3781 gcccgtagcg gcaggcacgc tcgcagtctt tctgggctat ggcctcggtc ggtatgtaac
     3841 tggaaatcca tggataggcg cggcgatggc cgcgcttgtg gcgatcgacc caatcagcgt
     3901 gcagtggagc ggccacgtgc ggatgtatgc cctgctgcag gtgttgaccg tgggccttgc
     3961 ctgggcgttc atccaccttc tcatgagggg tccgtcgaag cggcgcgtcg ccacagtcgt
     4021 tgccctcttt tggcttgccg tcttcaccca cgttggggcg actctcttgc ttcctgcgat
     4081 gctgctgacc gtcgtggtac tgacccgtcg agggttggtt ccgcgaaacg gttttctcac
     4141 cttgttcaca ctctgtggca tggccccatt gctgctcctg cttttgaatc gaatgcttgg
     4201 agtcacgagt ggggacctcg gtgagagttc gaggggccca gcgataccgt tcgtgggtga
     4261 caacctgatc gcacctttgg cccggttcca ggtgtcgccc gcggaatggg agctaaacgc
     4321 gctggcgaag ccttcaaccc tgttctggct catccccggg ctcatcgttg ccatctgctc
     4381 agtcattacc gcacgacgct acctcctcaa tcgcaactgc gcggcgtcag cagaaacccg
     4441 gatcggctgc acggtctgtt tcatcatgta ttggctgcct acgctgacgg tggctctgct
     4501 tacggcgtcg cccaagccaa ggtatctgct tcacgtgcac gtcctgggat acctgtttgt
     4561 agcggtgttg ctgtccaggc tcacacgggc tcattggtgt gaacgcgccg gtcggcgcaa
     4621 cgtcgtgggt tcggcgctcg cctgcagcgc catcgtcata gtcctggcag gaattggagg
     4681 agggctggtg tggcgcctgc aacacctggt ggtacatccg gattaccaca ctgcaatggc
     4741 ctatgcggcg tctaatcact cacccggaca accagtgatt gtggcactgc cgccggtggc
     4801 gtatctggcg atggatgcag ccgacaggga tgacctttat ttcctggctg gttcccgcca
     4861 aagcacacgc gctgaacgct acacgcttgc aaccgaggac ggcaggatcg tcgactactg
     4921 ggttggagct gaatccgtcg taacggcaca gggccttcgc agcatcctcc tcgagaaccc
     4981 tgactcgctc ctggtcatcg acgagcatcg gctcacggcc gaatgggcgt acggtggaaa
     5041 gatggaggat gttatcagag agatgactac ggtcgttcac aggggcccgg gcggggccct
     5101 cgtcctcacg ccatccgagg gtgggaagag cacaactcgg gacagagacc tgtttttcca
     5161 gagcggggga gagcaagggc ccggagccat tggaagcccc tgcatgcccg cgaggagctc
     5221 tcgagtatgc ggcggccaag catgacggat cagaggaccg attgatgtgg gtgccatcgc
     5281 gatcagaagg gactactggc gagagggtgc aatcccgcaa caaccccatt tccatccact
     5341 ggaatgatgt gatagcgcct gacacaagcc gaagcacatc ctgcaatccg gcgcctgact
     5401 gaggcgcagg caggccacta aaagattgat caaccgcaat tgacaccgta acgggccgga
     5461 acctatactg ctatggctcc gaacgggtaa cccgttggga agcataatag tggccgacat
     5521 taatagttgg ctgtgcgctg aacggaagta gtagacctcg atgtccagag agatgccggt
     5581 tggtgaaagg catcgacagg ggttgaactc gtccgggagc tgcgggggtg aatcagtagc
     5641 ccttgccgga atcctccgtt atcagggagc caagcggacc aaaagacggt tcgtgatcag
     5701 atgccacgcg gggccgtagc tcagctggga gagcaccaca ctggcagtgt gggggtcagg
     5761 ggttcgagtc ccctcggctc cacccgaa
//
LOCUS       DASYWM010000279         3540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_69558, whole genome shotgun sequence.
ACCESSION   DASYWM010000279 DASYWM010000000
VERSION     DASYWM010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69558"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VGR29_11360"
     CDS             complement(<1..491)
                     /locus_tag="VGR29_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV2074225.1"
                     /translation="MAAGRGERFGDAGKILANVCGRPVLAWVLDAMQAAGSVRDVVVV
                     HGDHTEAAIQSLLSSGAWTKVTALVPGGDQRQLSMANGVRAVAEDLEVVLVHDAARPL
                     ITPSLIDMCAERARQTGAAILATPMSDTVKRVEDGVVVATLDRSTLWAAQTPQGFVKE
                     RML"
     gene            complement(515..1381)
                     /locus_tag="VGR29_11365"
     CDS             complement(515..1381)
                     /locus_tag="VGR29_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV2074226.1"
                     /translation="MMVTESAQTRATIDLHTHTNASDGLDAPAELVANASQRGISVLG
                     VTDHDTMDGLDAASEAAAQAGITLVPGVELSTTVDRAEVHVLGYFVDSTESDLRARLS
                     DLAASRVRRVERMIVRLHELGYRVDGEAILAQAEEGSIGRPHVARALMEIGAVDSVND
                     AFERFLKAGRPAYIPRDPFSPEDAVGLLVRYSAIPVLAHPFSTKDIEGTLARLVPAGL
                     KGLETYYAEYSSEQHAQLHAIADGWGLVPTGGSDYHGLGFREGRELGAAPVPQDAWER
                     LSTLYADLAGGA"
     gene            complement(1392..1664)
                     /locus_tag="VGR29_11370"
     CDS             complement(1392..1664)
                     /locus_tag="VGR29_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HEV2074227.1"
                     /translation="MRVAGEVLKVSARSRPSAVAGAIAGVVRESGRAEVQAIGAGATN
                     QAVKAVAIARDYLAETGIDAVCVPAFIDVTIENEDRTAIRLVVEPR"
     gene            complement(2409..>3540)
                     /gene="rny"
                     /locus_tag="VGR29_11375"
     CDS             complement(2409..>3540)
                     /gene="rny"
                     /locus_tag="VGR29_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV2074228.1"
                     /translation="ERVAGLTATEARDQLIADIESEARHMAARRVHEIEREAKEDADR
                     RSRKILATTIQRIASDYVSESTVSVVPLPNDEMKGRIIGREGRNIRALEQATGVDLIV
                     DDTPEAVTVSGFDPVRREVARRALAKLLQDGRIHPARIEEVVYKTQLELEEIMREEGE
                     TIAYEANVPGLHPDLIKLLGRLKFRTSYGQNVLGHSLEVSLLCAALAAELGADVNVAK
                     TAGLLHDIGKAVDHEVEGPHALIGADIAKRLGRSSKIVHAIAAHHNEEEPQTIEAFIV
                     QTADAISGARPGARREMVETYIKRLEALEGVANSFEGVEKSFAIQAGREVRILVRPNS
                     VDDLGATRLAQDVVRKIEETLEYPGQIKVTVIRETRAVEYAR"
BASE COUNT          681 a         1197 c          985 g          677 t
ORIGIN      
        1 tcgagcatcc tctctttcac gaacccctgt ggcgtttgcg cagcccacag tgtggagcga
       61 tccagcgtgg cgaccacaac cccatcttcc acccgcttca cggtatcgga catcggcgtg
      121 gcaaggattg ccgcaccggt ctgccgggcc cgttccgcgc acatgtcgat caacgaaggg
      181 gtaatcaggg gtcgggcagc atcatgcacc aaaacaacct ccagatcctc cgcgactgcg
      241 cggacaccat tggccatgga gagctgccgc tgatcaccgc ccggaacaag ggcagtgacc
      301 tttgtccacg ctcccgagga cagaagggat tgtatggcag cttcggtatg gtcaccgtgg
      361 acgaccacca catcgcggac ggaaccggcc gcctgcatgg catcgagtac ccaggcgagc
      421 acagggcggc cgcagacgtt tgccaggatc ttgcctgcgt caccgaagcg ttcgccacgg
      481 ccggccgcca caatcacaca cccggcgaaa ccaatcatgc cccgccggcc aaatcagcgt
      541 aaagcgtgga aagtcgctcc cacgcgtcct gcggaacagg ggcggcaccg agttcccgcc
      601 cctcccgaaa gcccaagccg tgatagtcgc tgccgccggt cggcaccagg ccccacccgt
      661 ccgcgatcgc gtgcagctgc gcgtgttgct ccgacgagta ctccgcgtaa taggtctcga
      721 gtcccttcag gcccgcaggg accagcctgg cgagtgtccc ctcgatgtcc ttcgtagaga
      781 atggatgggc caataccggg atggcgctgt acctcacgag caggccaacg gcgtcctccg
      841 gtgagaaggg atcacgtggg atgtaggcgg gccgcccagc cttcaggaag cgttcgaacg
      901 catcgttcac agagtccacc gccccgatct ccatcagtgc gcgggccaca tggggacgtc
      961 caatgctgcc ctcttccgcc tgggcgagga tagcttcacc atccacccgg taccccagct
     1021 catgcaggcg gacgatcatc cgctccacgc gacgaacgcg agacgcggca agatcactga
     1081 gcctcgctcg caaatcgctc tcggtgctgt cgacgaagta tccgaggaca tgcacctccg
     1141 cacggtcaac ggtcgttgaa agctctacac cgggcaccag cgttattccg gcctgtgcgg
     1201 ccgcctcgct tgcggcgtcg agaccgtcca ttgtgtcgtg gtccgtcact ccgaggacag
     1261 aaatcccccg ctggctggca ttggcgacca gctcagcagg ggcatccaga ccatccgacg
     1321 cgttggtgtg agtatgaaga tcgatcgttg cccgagtttg tgcggattcg gtgaccatca
     1381 tactggcgtc actatcgtgg ctcgacgacc aaccggatcg ccgtgcggtc ttcattctcg
     1441 atggtgacat cgatgaaggc gggaacacag acggcgtcga tgccggtctc ggcgagataa
     1501 tcccgcgcga tcgctacagc cttgaccgcc tggtttgtcg caccggcacc gatcgcctgg
     1561 acttcagccc gcccggattc acggacaaca cccgcgatcg ccccagcaac cgcgcttggg
     1621 cgggaacggg cagagacctt gagaacctca ccggcaacgc gaataacctc gccttcggcg
     1681 atgtactcaa ccgtcgtgct cacctcatga aaactatggc cgttcgaggg tgctatcgcc
     1741 ggcgcagatt caagtggtgg cttcggctca aacggtgcgc tggttgcatc ccacgaagtc
     1801 tccgcgtccc ctgcgccgac ttccgtctcc gcgggagcat cgaaagaaac gacttcctgc
     1861 tcgatttcca ccgtgaccac ggactcggtg acctgctggt cagctccatc cttctcatcg
     1921 gcatccggcg gaaagctatt gccctccgga ttgatcttga tgtcctgcag attgagcttg
     1981 ctgaagaacc gattcatgcg tgagatgctc tctttccgat ttccattaac ccggagcccg
     2041 gatgctgtga tgccttcagg caaaacaaag ccaggcagga gatcctactc tccggcgtac
     2101 cgattgcgta acttgcccag cgaaagcctc ttctcccccg tcaagcccgg agaggtcctg
     2161 acgtgtcacc gccgagttgc ggtcacgccc ctagtctacg ctgcgactgc ccgttttcgc
     2221 acgaaaaaag cgtaacaata tggaaacagg cgccagtccc tcatggtgta ggccaagcag
     2281 aagacacaaa acgcgggtgg tcgcacctgt tttccaggtc acaaccaccc gcgtgtcgtc
     2341 atcaaaacgc gtaaactatg cgtcatcgcc ggcaggtacc ccataccgta tcagcgatgc
     2401 ctctctcctt accgcgcgta ctcgaccgcg cgggtttctc gaattaccgt gaccttgatc
     2461 tgtcccggat actcgagcgt ctcctcgatc ttccgcacga catcctgcgc aagacgcgtt
     2521 gcgcccagat cgtcaaccga gtttggcctg accaggatcc ggacctcacg gcccgcctgg
     2581 atcgcaaacg acttctcaac accctcaaac gagttggcga cgccttccag cgcctcaagc
     2641 cgcttgatgt acgtctcaac catctcacgt cgcgcaccag ggcgtgcacc tgagatggcg
     2701 tcagccgtct gcacgataaa cgcttcgata gtctgcggct cttcctcgtt gtgatgtgcc
     2761 gcgatggcat gcacgatctt ggaggatcgt cccaaacgct tggcgatatc cgcgccgatc
     2821 agcgcatgcg ggccctccac ttcgtgatca actgccttgc cgatgtcatg caacagaccg
     2881 gcggtcttcg ccacattgac atccgcgccg agctcagccg ccaaggctgc gcatagcagt
     2941 gacacttcta gcgagtgtcc cagcacgttc tggccgtaac tcgtccggaa cttcaatcgg
     3001 ccgagaagct tgatcaggtc agggtgcaga ccgggcacgt tcgcctcgta ggcaatcgtc
     3061 tcgccctcct cccgcatgat ctcctcaagc tcgagctggg tcttgtagac gacttcctcg
     3121 atccgcgccg gatggattcg gccgtcctgc agcagcttcg ccagtgcgcg acgtgcgact
     3181 tcacgccgga ctggatcgaa cccggaaacc gtcacggcct ccggcgtgtc gtcgacgatc
     3241 aagtccacac ccgttgcctg ctcgagcgcc cggatgttac gaccctcacg gccgatgata
     3301 cggcccttca tttcgtcgtt cggaagcgga accacgctca ccgtggactc cgacacgtag
     3361 tcggacgcaa tgcgctggat tgtggttgcc aggatcttac gcgaacgcct gtcggcatct
     3421 tccttagctt cccgctcaat ctcgtgcacg cggcgcgcgg ccatgtgccg tgcctcgctc
     3481 tcaatatccg caatcagttg atcgcgggct tcggtagcag tgagcccggc cacccgctca
//
LOCUS       DASYWM010000280        18456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_482614, whole genome shotgun sequence.
ACCESSION   DASYWM010000280 DASYWM010000000
VERSION     DASYWM010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 18456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18456
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482614"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="VGR29_11380"
     CDS             complement(<1..1323)
                     /locus_tag="VGR29_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV2074229.1"
                     /translation="MTLLVQAANIVYAHGGNQIFEDTSFVLRQGDRVALIGDNGSGKS
                     TLFRLLAREISPASGVVTHRRGLTLGYLHQQSTLDPTRTVREVVARAAGDPTALEDQL
                     RALEDQLTQPLDDGAMADVVDRYSATLERLDAVQNTDHQAALNDVLGGLRFPEHRWDQ
                     RIGDLSGGERKLVDVAQFLLNQPDVLLLDEPDNHFDIDAKAWLEHWLTTTYTGAVCLI
                     SHDRFMIDQVTNTIFELEDGRIRQYPGNYTTYLNLRQARLERTAELRELQEREFRKLK
                     ASAEQLTQWARQNPKFASRAENQRRKMAEERARLESEPAPVLNRRQIQVEFATERGST
                     DVLTAEGVAKSFGEREVFKPFDLLLRHGERVGLVGPNGAGKTTLVRMVLGQELPTRGT
                     IRIGSSINVGYYAQQHETLDPNATPLDMVRKTKPLTEQQALSFLVGLLF"
     gene            complement(1444..2301)
                     /gene="prmC"
                     /locus_tag="VGR29_11385"
     CDS             complement(1444..2301)
                     /gene="prmC"
                     /locus_tag="VGR29_11385"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV2074230.1"
                     /translation="MTIQETLHRATSRLAAGGIETARLDAEVLLRHVLGVDRTQLFLR
                     YPDALPEDTDAAFEALVRRRLDGTPVAYLTGTREFMALPFQVGPGVLIPRPDTEPLVE
                     WALGWLMDRPTARVADIGTGSGAIAISLAAHLPSTFAGEIVAIDTSPDALGVARRNAD
                     NLLPPPRQAWLRVAEGPLTRTLTGQVDLLLANLPYLTPKQISENPDLDAEPRLALDGG
                     ADGLDLIREVIADLPRVLSSGGAAGFEIDPAQEDSVVSLLQDTIPDAKVHTVLDLAGS
                     SRHVVVTRS"
     gene            2691..2891
                     /locus_tag="VGR29_11390"
     CDS             2691..2891
                     /locus_tag="VGR29_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074231.1"
                     /translation="MHRVFSMWILAGAFRPVCYVVCIVAGLYLLEMIVEIIWPTSGKD
                     DDVDDLLARVQRMENCAGAHPS"
     gene            2965..3306
                     /locus_tag="VGR29_11395"
     CDS             2965..3306
                     /locus_tag="VGR29_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074232.1"
                     /translation="MTSHDDTENEMPVTSEEGAELGSVSSESEASVAGSQAADPDKSS
                     GNPEEDVSAASVIPDEMLKHENPAAAAMLEMQQQQATLASRQVGGQTQDRCPCCGQPI
                     RDAQGGVPTSE"
     gene            complement(3917..5026)
                     /gene="prfA"
                     /locus_tag="VGR29_11400"
     CDS             complement(3917..5026)
                     /gene="prfA"
                     /locus_tag="VGR29_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV2074233.1"
                     /translation="MAASMRSFSATLDELQRRLSEVDHMMADPSVATDADKLMELGRE
                     RSDMEPLVTAWSEYKSIEEQIDEARVMAEDTDADLADLAREELKSLRQQRDELEAAIR
                     RMLVPKDPNDEKNVIVEVRAGTGGDEAALFAADLYRMYTRYAERSKWKVEVLSSTPTD
                     GGGFREIIFEVKGKGAYSYLRYESGVHRVQRVPATESQGRIHTSTASVAVLPEAEEVD
                     VQINDNDLRIDVFRSTGHGGQSVNTTDSAVRITHMPSGIVVSMQDEKSQLKNKIKAMS
                     VLRSRLYEQEQQRLAGERGDVRRSQIGSGERSEKIRTYNYPQDRITDHRIGKSVSNIP
                     AVMNGQLDVLIEDLRAADEAERLSEAGMGEAAAAD"
     gene            5179..5886
                     /locus_tag="VGR29_11405"
     CDS             5179..5886
                     /locus_tag="VGR29_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074234.1"
                     /translation="MQQEIDSTPDLCTFTPVDVVVLRYRRNRPADLVIPGRVAEDAAS
                     HIALFTMSGTVLEGQATASGKRLTRAMPFLERERIVNGMADFTWRRNHMLQLILPGEA
                     RATWLLWSDHHWSLQAWYVNLQAPIQRTEWASTWLIICLIDITPNLQWSWKERDVVDE
                     TRRHGLVAPEIHDRMEREGERAIRDIEAWAWTSDADNEHWRLIPHGGSRHFWMGEMPG
                     WTDRLYSSCKGIMSPEG"
     gene            5904..6524
                     /locus_tag="VGR29_11410"
     CDS             5904..6524
                     /locus_tag="VGR29_11410"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV2074235.1"
                     /translation="MRARREPGDQIEVEFIVGEEPFLVMPVTVVEDSDTRIAHYLAAG
                     TRYLRRVLMDGSPVPRVMPLDELRRVGSRLMEDEWRGSHRLIVTRPGRAHAVYLRWSA
                     ATWEFNGWYVNLQEPLQRTESGFTTEDHFLDILVAPDVTWQWKDEDELEEAVAVGRVT
                     RAAADTIRLEGERVIDDIEAERFPFDNSLRLWRPDQAWRIAALPSS"
     gene            6690..8651
                     /locus_tag="VGR29_11415"
     CDS             6690..8651
                     /locus_tag="VGR29_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor GTPase family protein"
                     /protein_id="HEV2074236.1"
                     /translation="MHTLNLGILAHVDAGKTSLTERILFETGVISEVGRVDRGTTQTD
                     TLELERQRGITIQSAVASFRLGDLSVNLIDTPGHSDFIAEVGRALQVLDAVILVVSAV
                     EGVQPQTRRLVRAVRSSGIPLLLFINKIDRAGARSDSLIAEIRRTLGLCPLVLSTVSD
                     LGTRSAAVHPHELDDQSFAAGVLDLLAEHNDAVIAQYISSGDSLPLETLRAEISRQFH
                     DRHIVPAFFGSAITGAGVSNLLDELPQLVGASRDRCTEPLSGVVFKIQRTPHGEKVGL
                     ARLFSGKLNVREHVVFRRTLATGQSASYGARITGIERYHPGGIAIVSEAHAGDIVRLH
                     GVQELQVGDVSGEVLPHTSTATFPPPTLESVIHARDAVDKARLYAALEQLEEQDPLIS
                     IRRGDRDSSISLRLYGEVQKEVIESTLHNDYGVAVDFGTSQTICIERVVGSGSAAEEM
                     GGANPFQASVAFSVEPGAPESGITYRRELGSLPLAFYRAIEETVYETLREGLHGWEVR
                     DGVVTLTKVGFSSVSSTAGDFRKLTPLVLMQALERAGTEVCEPIERFVLEVPKDAVGE
                     VYRMLSFARATPEEATPDGDMSRVTGTIPSSEVHVFEQHLSALAHGEAVFTSTHEGYR
                     RMRGSWPERTRTDFNPLNRTLYLALVSQS"
     gene            8833..10029
                     /locus_tag="VGR29_11420"
     CDS             8833..10029
                     /locus_tag="VGR29_11420"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2074237.1"
                     /translation="MKRLGVIGYGGRIRHMLGEIDRYDTGATVVAIIDPAEARLREQF
                     PEQLENVTFYDDVDRMLDEAEVDGVLIGTQCVHHTPYAIKVLQRNLPLFLEKPVAINE
                     QQLAELFTASRESSSETVVSFPLRLSHLCQMTREILDSGVIGTIENVQAINNVPFYGS
                     NYYHGWMRNEDLTGGLWLQKATHDLDYLTYLIGKPPARIVAVESKTVFTGEMPAGLYC
                     LECPVNDTCPESQKNLFYMQGILDDMHKAEEWWDGRWQCSFAVDTGNHDAATAILQYE
                     SGAHAVYTQNFYARRGAAKRGATFIGYKGTVSFDWVTSEVVVHHHHTPRVERHVLGGG
                     DGGHHGGDRELAHDFLAILNGTGRSRATLEDGLLSAQLCMMAKRSCQTSGFETYHPLR
                     STATVR"
     gene            10250..11224
                     /locus_tag="VGR29_11425"
     CDS             10250..11224
                     /locus_tag="VGR29_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074238.1"
                     /translation="MNFELKRPGRSRFLQRPMNRRKLLHASALSTAGGLLLPGASGAQ
                     ESSLDLPPSQNTSTPSAPIGARFNEGLLPDHRLIMYYGFPENPNMGILGEYPPEQLLP
                     QLLEQKAEYEAVINDRPWKAGFELIASVAQREPGPDGKYIADTDGKWLDMYTQFTEAN
                     DMFLVLDVQMGLKTPKEDYEGLERWLRYDHVHLAIDPEFHVLPGETPGIELGSIDAAD
                     VAEAQAWLLQLAEKYGTSRKMLLIHQFNTYSVSNKDQIKPLEGVDLVLNMDGWGPPWQ
                     KLETWEVIIQQTPIEYNGVKVFYRQDEPIMTPAEVIALEPTPDVINYQ"
     gene            complement(11386..12093)
                     /gene="thpR"
                     /locus_tag="VGR29_11430"
     CDS             complement(11386..12093)
                     /gene="thpR"
                     /locus_tag="VGR29_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEV2074239.1"
                     /translation="MENNDGQQPQRRTPKQRRQRFVRERPPEESEDTPWRLFIAVPLP
                     DEVRAVVARIIADLRGNDWPIRWTDPDNAHLTLHFLGDTAPENAELLRLALGEAIAPH
                     AAFDLRTADLGAFPSIKRPRVLWLGLWGPAHRLDTIRNDIGSLLQSFEVELDEKEFRP
                     HITLGRVRDSRTIRVRDLPGTIRTRFEELATSGEVTHEKPVPFPVREVHLVRSHLSRE
                     GARYEVIGRYPLKASKS"
     gene            12177..13031
                     /gene="pheA"
                     /locus_tag="VGR29_11435"
     CDS             12177..13031
                     /gene="pheA"
                     /locus_tag="VGR29_11435"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV2074240.1"
                     /translation="MAVAYLGPRGTNSEEAAILYAGPEAELEPFSGMPEVIQAVESGA
                     ARAGVLPVENSIEGPVSVSLDLLIHTSNLKLCHEVVVPIRHVLVAKKGARPQGIRRVM
                     SHPQALAQCRLFLEREFPQAEQVAWMSTGGSVQEVARGDDLTVAAIGPERAQHLYGGE
                     VIARDIQDARSNLTRFMVLAHHDAEPTGDDKTFIALKTDKDEPGSLFRMLEPFAKAGI
                     QLTNVITRPTKGWLGEYVFLLDFQGHRKDPVVAKLLDDVREVADELKIIGSCPRFPVE
                     QYRDLLAG"
     gene            13120..13917
                     /locus_tag="VGR29_11440"
     CDS             13120..13917
                     /locus_tag="VGR29_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEV2074241.1"
                     /translation="MTLYIDESLMTAQVRIPLVEGWVETDVPFEIRRNLQAADIGSSD
                     VALIAGPEAAFLGETHFIEPEIAVVAEAMSAIVMRTPVRPDGVEETPIRMLGTGPTAE
                     LLIRALLRPYFGITASTFVRDDDDPSAADAQVVILEGLLALNEPEAGFQEDLAKAWYI
                     LTNAAVVHHVTVIGVEAEARGATTEIDLLKRAVQAGVERRRDVRREMVGDEEIDRDRL
                     AELTNRIRFELSTADRQSLRNLLARGSWGTSYLRALPAFRDELTSPE"
     gene            complement(13932..14414)
                     /locus_tag="VGR29_11445"
     CDS             complement(13932..14414)
                     /locus_tag="VGR29_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEV2074242.1"
                     /translation="MSGGDHLPQLFTVTENVLDPRSVESLVAGPDSGAIVTFIGTVRD
                     NARGLTVTALDYEAYPSAAEKMLARVGTEVADQWPEVRIAISHRIGHLEPGEASVVIA
                     CASPHRENAYDASSFAIARIKEIVPIWKKEWYEDGSSWIGSELDYQIETGRIDTPSGT
                     "
     gene            complement(14403..14645)
                     /gene="moaD"
                     /locus_tag="VGR29_11450"
                     /pseudo
     CDS             complement(14403..14645)
                     /gene="moaD"
                     /locus_tag="VGR29_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
     gene            complement(14756..14983)
                     /locus_tag="VGR29_11455"
     CDS             complement(14756..14983)
                     /locus_tag="VGR29_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074243.1"
                     /translation="MTHKEGRFGVSDVEYNIVTTLSNLLQAEDVLNRYAKDAEEAGES
                     EVSELFRELEQVNNQFASRLRDRLKTVLDQG"
     gene            complement(15157..17256)
                     /locus_tag="VGR29_11460"
     CDS             complement(15157..17256)
                     /locus_tag="VGR29_11460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV2074244.1"
                     /translation="MGNKESKTGKKAARAVKAKGKRDSLKQMRRGLDLEVKRTSAVKH
                     ASSAPERTGRPLKIDDLYKWSLVGDPRVSPDGTRVVAPVLTIDRESDEYRTALWNIPL
                     NGDAPTQLTSGQWTDSSPRWSPDGSWIAFTSNRNEKKTQIFVLPTAGGEAIQVTRGEN
                     EVGEFSWAPDSDRIVFTSRIGPEKEDESESDVKVITSARYKFDGEGFLEDKVSQLYVV
                     RVSTPEEEPTRLTEGQFNHTAPAWSPSGHEIAFVANRDDNWDKSRKNDLWTVNPNGGE
                     PRKLTDGQGSWRAPVWSLDGTSIAITGNADVELHEVNSWLYIIPAAGGKPERVGKELD
                     RYVGDSSMSGPNGPAGAPLRWLSDGSAIDALLSDRGATVVMRFRLNGKRPMQLTGPDR
                     HVTGFDHISSDELVISVTDPTTPAELRRVTAEEETPLTSFNAEWLGQVAIPTPEEFWV
                     KSNGEAIQGWLIRPAGNSSDGAVVPLILNIHGGPHAQFSNAWSHELQMYVARGNALVY
                     INPRGSTGRTDAFAQAVNGAWGIADMPDFMAAVDHVLSLGGLDPERLGVTGGSYGGFS
                     TNWLLGHTDRFRTAVTDRSISNMISMYGTDDIALVSLDPELGTPWDNFKTYWKLSPLK
                     YVENITAPLLIVHSEEDYRCPMEQAEQLFIALKKLDRTVELVRFPNESHGLGRNGKPK
                     HRVERLERTLGWFETYL"
     gene            complement(17346..>18456)
                     /gene="glp"
                     /locus_tag="VGR29_11465"
     CDS             complement(17346..>18456)
                     /gene="glp"
                     /locus_tag="VGR29_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEV2074245.1"
                     /translation="QDITAPEDHPPFPASTMDGYAVFSQDHSPWREIIGTQNAGDTID
                     AEVTEGYAVRIMTGAPLPPGADAVVPVEATELMEDHVVIHQEDVAIGENIRPVGSDVA
                     RGTLVLQAGTRLGPAEIGLVASMGISPVPVARRPRVSVISTGDELVEPDQPIGPGQIR
                     DSNRFSLIAALLAEPVEITFAGKAPDKLDQLEALLRERMATDDIIITSGGVSMGERDL
                     IKAILFDAGDVHVHFRRLFMKPGKPLNFATHGDTLIFGLPGNPVSSLVTFELFIRTAI
                     RQMIGETEVDRPVVRVTLVEGTSPSDRIEYQRGVVSAERNGKLIARSTGLQRSSRLAS
                     FLGANALLVIAPRETDYAAGETIDALMLGAPIPAL"
BASE COUNT         3513 a         5359 c         5886 g         3698 t
ORIGIN      
        1 gaagagcaac ccaacgagga acgacagcgc ctgctgttcc gtgagcggct ttgtcttccg
       61 caccatgtcc agtggcgttg cgttgggatc gagcgtctcg tgctgttgcg cgtagtagcc
      121 gacgttgatg ctggagccga tgcgtatagt tccccgagtt ggcagctcct ggccaaggac
      181 catgcgcaca agcgtggttt tgccagctcc gttcgggcca accagcccga cgcgctcccc
      241 gtgacgcagg agaaggtcga atggcttgaa gacctcacgc tcgccgaagc tcttggccac
      301 gccctcggct gtgagaacgt ctgttgatcc gcgctccgtt gcgaactcca cctgaatttg
      361 gcgccggttg aggacgggtg caggctctga ctccagccgt gcccgttcct cagccatctt
      421 gcggcgttgg ttctcggccc gtgaggcgaa tttcgggttc tgtcgtgccc actgggtgag
      481 ttgttcggcg ctcgccttca acttcctgaa ctcgcgctcc tgcagctcgc gcagctcggc
      541 ggtccgttcc aggcgtgcct ggcggagatt cagatacgta gtgtagttgc cgggatattg
      601 ccgaattctg ccatcctcca gctcgaagat cgtgttcgtt acctggtcga tcatgaagcg
      661 atcgtgcgag atcaggcaga cggcacctgt gtaggtcgtg gtcagccaat gctccagcca
      721 cgccttagcg tcaatgtcga aatggttatc cggctcatcg agcagcagca catccggctg
      781 gttaagcagg aactgcgcga catcgaccaa cttgcgctcc ccaccggaga ggtcgccgat
      841 gcgctgatcc cagcgatgct cgggaaagcg cagaccaccg agcacgtcat tgagcgcagc
      901 ctggtggtca gtgttttgca ccgcgtccag gcgctcaagc gttgcactgt accggtcgac
      961 gacgtccgcc atcgccccat cgtcgagtgg ctgcgtcagt tgatcctcca gtgcccgaag
     1021 ctggtcttcc aacgcggttg gatccccggc tgcacgcgcg acgacctcac gcactgttcg
     1081 ggtggggtcg agtgtcgact gctgatgcag gtacccaaga gtgagcccgc gacggtgcgt
     1141 gaccacgccg ctggctggcg aaatctcgcg tgcaaggaga cgaaagagcg tgctcttgcc
     1201 actgccgttg tcgccaatga gtgccacacg atcgccttga cgcagcacga acgacgtgtc
     1261 ctcaaaaatc tggttgccgc cgtgggcgta cacaatgttg gcggcctgaa caagcagtgt
     1321 catggagtgc cctgtcctgt cctttcgttg cccgagcgcg attgccggcg attcctcagt
     1381 tgaggcgttg gtctgcggtt atgcagtgcc gcctattgtc gcaggtcgaa gttgcagtgg
     1441 gccttacgaa cgggtgacca caacatgccg cgatgatcca gcgaggtcaa ggacagtatg
     1501 gacctttgca tcgggaatcg tgtcctgaag gagtgacacg acgctgtcct cctgggcggg
     1561 atcaatctcg aagccggcgg ccccgccaga cgagagcact cttggcaggt cggcgatcac
     1621 ttcccggatg aggtcgaggc cgtctgcccc gccgtcgagc gccaggcgcg gttcggcgtc
     1681 cagatccggg ttttccgaga tctgctttgg cgtgagatag ggcaggttgg ccagaaggag
     1741 atcgacttgc ccggtaaggg tgcgggtgag cggaccctcg gcaacgcgta accaggcctg
     1801 cctggggggt ggaagaaggt tgtcggcgtt gcgtcgagca acccccagtg cgtcaggaga
     1861 tgtgtcgatg gcgacgatct caccggcgaa ggtggacggc agatgagccg caagggaaat
     1921 cgcgatcgcc ccggagcccg tgccgatatc ggccactcgt gctgtcggtc ggtccattag
     1981 ccatccgagc gcccactcaa ccagcggctc cgtatccggt cggggaatga gcacgcccgg
     2041 tcctacctgg aagggcagcg ccatgaattc ccgggtaccc gtaaggtagg ccacgggtgt
     2101 gccatccaga cgccgccgga ccaaggcctc gaatgcggca tctgtatctt ccggcaatgc
     2161 gtcagggtaa cggagaaaga gctgcgtgcg gtcgacgccg aggacatggc gcaacagcac
     2221 ctcggcatcg agtcgggcgg tttcgatgcc ccccgccgcg aggcgggatg tggcgcggtg
     2281 gagcgtttcc tgaatcgtca tgggtggtcc aacacagagg ggcggcttcg ggtcgcccct
     2341 gcacgagtga ctatcggcta ctaccgagtt cggcactacg gctgacgagc tcctgtccga
     2401 tgctgagcgg atcgcgtcat gagtgaaccc ggggccgtgt ctggctgaat ccgcaagtcc
     2461 acagacgagt agatgtatca gcagaattca cccggatggg ggacgctata gccgcacgca
     2521 gtcaataata ctcccatcga aggtgtgcat tgagctgggg acaccttcac aacacttcct
     2581 caacgccccc ctttgctttc cggcgggggg cgttgctgtg tcctttaatc ttggccccgc
     2641 cgtgggcccg gtctctaagg cgccaggacg cttccggaaa tcgtgtctgt gtgcaccgag
     2701 tgttcagtat gtggatactt gcgggcgcct tcaggccggt ctgttacgtc gtctgcatcg
     2761 ttgccggcct ttacctgctt gagatgatcg tcgagatcat ttggcccacc tccggcaagg
     2821 atgatgacgt tgacgatctc ctggcacgag tccagcgaat ggagaactgc gcgggtgcgc
     2881 acccgtcgtg aggtgatgtc cctcccaggc acttgcaagg ggaccatctg atttcttgcc
     2941 tcatcagcaa tagaaggaat atcgatgaca agccatgacg ataccgagaa cgagatgccg
     3001 gtgacttcag aggagggggc ggaattgggg agcgtgagtt ccgaatccga ggcgagcgtg
     3061 gcgggaagtc aagccgccga tcctgacaag tcatcgggaa acccggaaga ggatgtctcc
     3121 gcggcaagcg tcattcctga cgagatgttg aagcatgaga accccgccgc ggctgccatg
     3181 ttggagatgc aacagcagca ggcaacgctg gcgtcccggc aagttggagg gcagacccag
     3241 gaccgctgcc cctgttgtgg gcaaccgatc cgggatgctc agggaggggt ccccacgtcg
     3301 gaataggctc gccggcttct tgacatgcca tcccggaaac tgttaggacg acgcccctcg
     3361 ctcgaatgcc gggggcgttt gtgtttatcc tgatcacgta gccaattgtt ttcaactcag
     3421 gaggttggcc gtggaaggcg cagcagccgt tgcttgtgag ctgtgtgatg caaaactcac
     3481 cgtctccgat gaagcacttc gagacccaaa taccctggag caggatctgc cccctggctg
     3541 gacactgtac gtgagacggg atgcgagcac cggcgagtat atccgggtgg tgcgttgccc
     3601 ggaccaccaa ccctagctgc ccaaaatttg gcagtcccgc gattcggaca tctcgtggga
     3661 atcgcctgga ttgatcgctc gttacccgcg tgagtgcgcc agagcgcaca gggaacggat
     3721 cctggataag taggggtact cttgccaatg cgctgaagac cagcctacac gtcatcgccg
     3781 ccggggcctt ggagccgctt accaacgcca ttggggtggg gctcgattag atcagtgcct
     3841 ctgatatacg cggcgtccat cggcattgcg gccatccgct tgcgaccgtc caacaccaac
     3901 cgctgtggaa acggctctaa tctgctgcgg cagcctcgcc catgccggct tccgagagtc
     3961 gttcggcctc atcggcggcg cggagatcct cgatcaggac gtcgagttga ccgttcatca
     4021 ccgcggggat attgctgacg ctcttgccga tgcggtggtc ggtgatgcgg tcttgcggat
     4081 agttgtaggt gcggatcttc tcgctccgct caccggagcc gatctgcgaa cgccggacat
     4141 caccgcgttc accggcaagc cgctgctgct cctgctcata gaggcgggag cggaggacgc
     4201 tcattgcctt gatcttgttc ttgagttggc tcttctcgtc ctgcatgctc actacgatgc
     4261 cggagggcat gtgtgtgata cggaccgcgg agtcggttgt gttaacgctc tgtcctccgt
     4321 ggcccgtgga gcggaagacg tcgatgcgca ggtcgttgtc gttgatctgg acgtcgacct
     4381 cctcggcttc gggcaggaca gcgacgctgg ccgtggaggt gtggattcgg ccctgcgatt
     4441 cggtcgcggg cacgcgctgg acgcgatgaa cgccggactc gtagcggagg tagctgtagg
     4501 ctcccttgcc cttgacctcg aagatgatct cccgaaaacc gccgccatcg gtcggggttg
     4561 aggagagaac ctccaccttc cacttgctgc gctccgcgta gcgcgtatac atgcggtaga
     4621 ggtcagccgc gaagagggct gcctcatcgc caccggtacc ggcgcggact tctacaatga
     4681 cgttcttctc gtcgttcggg tccttcggca cgagcatgcg tcggatcgcg gcttcgagct
     4741 cgtcacgttg ctggcggagg gatttcagct cctcgcgggc gagatccgca agatcggcgt
     4801 cggtgtcttc cgccatcact cgtgcctcgt cgatctgttc ctcgatcgac ttgtactcgg
     4861 accaggcagt gacgagcggc tccatgtcag agcgttcgcg accgagctcc atcaatttgt
     4921 cagcgtcggt cgcgacagaa ggatcggcca tcatgtggtc gacctcgctg agccggcgtt
     4981 ggagttcgtc cagggtggcg ctgaaacttc gcatggatgc agccatgacg gtcattactc
     5041 cgggtgttgt ttgcgttggg atcggcggtc ctggatgctg gcatattgga acgacggcgt
     5101 tcaaaagacc gatacacaaa gtataaaccg ttaccagagc ggtcactcgc atgccaagac
     5161 gcagtgtcag gatcagccat gcagcaagaa atcgacagca cccccgattt gtgcaccttc
     5221 acgcccgtgg acgtggtggt gctgcgttac aggagaaatc gaccagctga ccttgtgata
     5281 ccggggcggg ttgccgagga tgccgcgtcc catattgccc tctttacaat gtcgggaact
     5341 gtcctcgagg ggcaggcaac cgccagcggg aagaggctga cccgtgcaat gccttttctg
     5401 gaacgggaac ggattgtcaa cgggatggcg gatttcacct ggaggcgaaa tcatatgctg
     5461 caactgatcc tccccggtga ggcgcgtgcg acatggcttc tgtggagcga tcatcactgg
     5521 tcgttgcagg cctggtacgt caacctccag gcgccaatcc agcggacgga gtgggcttcg
     5581 acatggctga ttatctgctt gatcgatatc acgccgaatc ttcaatggtc gtggaaggaa
     5641 cgggatgtag tcgacgagac acggcggcac ggccttgtcg ccccggagat ccatgatcgg
     5701 atggaacggg agggggaacg cgcgattcgg gatatcgagg cctgggcatg gacctccgat
     5761 gcggacaatg agcactggcg actgatccct catgggggat cccggcattt ctggatgggg
     5821 gagatgccgg gctggacaga ccgtttgtac tcctcttgta agggcattat gagcccggag
     5881 gggtagtcca agggtgggat gcgatgaggg caagaagaga gccgggtgac cagattgagg
     5941 tggagttcat cgttggggaa gaacccttcc tcgtgatgcc tgtcacggtc gtcgaggaca
     6001 gcgacactcg cattgctcac tacctcgccg ccggaacccg gtatctccgc cgggtgctga
     6061 tggatggttc accggtgccc cgggtgatgc cactcgacga gcttcgccgc gttggctccc
     6121 gtctgatgga agatgagtgg cgaggttcgc accggctgat cgttacccga ccgggccggg
     6181 cgcatgccgt ctacctgagg tggagcgcgg ccacctggga gttcaacggt tggtacgtca
     6241 acctgcagga gcccctgcag cggacggaaa gtggattcac cacggaggac cacttcctgg
     6301 acatccttgt agccccggac gtcacttggc agtggaagga tgaggatgag ctggaggagg
     6361 cagtcgcggt cggtcgagtc acccgagccg cggccgacac cattcggctg gagggtgagc
     6421 gggttatcga cgacatcgaa gccgaacggt tcccgttcga caacagcctc aggctatggc
     6481 ggcccgacca ggcatggagg atcgcagccc tgccttcgag ctgagttctt gtagaaaaga
     6541 actcaatgca aaacggttgc gctatcgact cctccaccat agaatcgggg tactggttca
     6601 tcaactgaag ccctccgagg aggttcccat gcggcaaaca tcgagattcc ccatgtccat
     6661 gacattgctc gatggaggct ctttgccgca tgcacacact caatctggga atcctggcgc
     6721 acgtcgacgc cggaaagacg agtcttactg aacgcattct ttttgaaact ggtgtcatct
     6781 ccgaggtcgg ccgcgttgat cgcggcacga cccaaaccga tacgctggag ctggagcgcc
     6841 agcggggcat aacaatccag tcggccgtcg cgtcgtttcg ccttggtgat ctctccgtca
     6901 acctgatcga tacgcccggt cactccgact tcatcgcgga agttgggcgt gcgctacagg
     6961 ttctggacgc tgtgatcctg gtcgtctctg cggtggaggg agtccagcca cagacccggc
     7021 gcctggtgcg agcggtccgt tcatccggga ttccgctcct gctcttcatc aacaagattg
     7081 accgagccgg agcacgtagt gacagcctga tagcggagat caggcgaact ctcggcctgt
     7141 gtccgctggt gctctccact gtgtcggacc tcgggacgcg atcagcggcg gtgcatcctc
     7201 acgagctcga tgatcagtcg ttcgctgctg gcgtgctcga cctgcttgcg gagcacaatg
     7261 acgcggtgat cgcccagtac atttcgagcg gtgactcact tcccctcgaa acgctgcggg
     7321 cagagatcag tcgccagttc cacgatagac atatagtgcc cgcctttttc ggatcagcca
     7381 taactggtgc gggagtatcg aacctgctcg atgagttgcc acaactggtg ggagcgagcc
     7441 gagatcgctg cactgagccg ctttcaggtg tcgtgttcaa gatccagcgg actccgcacg
     7501 gcgaaaaggt aggactggcg cggctcttct ctggcaagct gaatgtccgg gagcacgtcg
     7561 tttttcgccg cacgttagca accgggcagt ccgcctctta cggagctcgc atcaccggga
     7621 tcgagcgata tcaccctggt gggattgcga tcgtgagcga ggcccatgcg ggtgacattg
     7681 tgcgtctcca cggtgtccag gagttgcagg ttggagacgt atcgggtgaa gtgctgcccc
     7741 atacatccac tgcgacgttc ccgccgccaa cgctcgagag cgtgatccat gcacgtgatg
     7801 cggttgacaa ggcgcggctg tatgccgcat tggaacaatt ggaggagcag gatcccctca
     7861 tcagcattcg gcgtggcgat cgggacagca gcatctccct tcgcctctat ggcgaagtcc
     7921 agaaggaggt catcgaatcg acgctccaca acgattacgg tgtggcggtg gacttcggca
     7981 caagccagac gatctgcatc gagcgagtcg ttggcagcgg ttcagcagcc gaggagatgg
     8041 gtggtgcgaa tccgttccaa gcttcagtcg ccttcagtgt cgagcccggg gctcctgaga
     8101 gtgggatcac ctaccgacgc gagctgggct cgctccccct ggcgttctac cgggcgatcg
     8161 aagaaacagt ctacgagaca ctacgagagg gactgcatgg ctgggaggtg cgcgacggcg
     8221 ttgtgaccct gacgaaggtc ggctttagtt ccgtcagcag caccgccggt gatttccgca
     8281 agctcacgcc tctggtgctg atgcaggcac ttgaacgggc gggcacggaa gtttgcgagc
     8341 cgatcgaacg gtttgtgctt gaggttccca aggatgcggt tggcgaggtg tacaggatgt
     8401 tgtcctttgc tcgtgcgacg ccggaggaag cgaccccgga tggtgacatg agccgggtga
     8461 cgggcacaat accctcctcg gaagtacacg tctttgaaca gcacttgtcc gcactcgcgc
     8521 acggtgaggc ggtcttcacc tctacgcatg agggatatcg acggatgcgg ggcagctggc
     8581 cggaacgtac ccggacagat ttcaatccgc tcaaccgcac actctacctg gcactcgtga
     8641 gtcagtcttg aacttggtgg gacggaagtt cgcgggtccg tttgctgatc gctcccggga
     8701 gcctgtgccg atgcggcgtt gcctttgatt cacgacgatc aaaggtatgg cttctgtctt
     8761 gacatcacgt cgcgggggat tgaccatctt cacgaatgag ttggcagcgg tctgaggatg
     8821 ggaatgacgt tcatgaagcg gcttggtgtg attggatacg gcggacgcat ccgccacatg
     8881 cttggcgaga tcgatcgcta cgacaccggt gccacggtcg tcgcgatcat cgaccccgca
     8941 gaagctcgat tgcgggagca gttccccgag cagcttgaga acgtcacctt ttatgacgat
     9001 gtcgatcgaa tgctggacga ggcggaggtc gatggtgtcc tgatcgggac gcagtgtgtc
     9061 catcacaccc cgtatgcgat caaggtattg cagcgaaacc tgccactctt ccttgagaag
     9121 ccggtggcca tcaacgagca gcagctggcg gagctcttca cggcgtcacg ggagtcgtcg
     9181 agcgagacgg tggtctcctt cccgttacgc ctctcacacc tgtgccagat gacgagggag
     9241 atcctcgaca gtggggtgat cggcacgatc gagaacgtgc aggcgatcaa taatgtcccg
     9301 ttctatggct cgaactacta ccacggctgg atgcgtaatg aggacctgac agggggcctc
     9361 tggctgcaga aggcgacgca cgacctggat tacctcacgt acctgatcgg taagccaccg
     9421 gcccggatag tcgccgtcga atccaaaacc gtctttaccg gggagatgcc agcgggcctc
     9481 tattgtcttg aatgcccggt gaacgacaca tgccccgaga gccagaaaaa ccttttctac
     9541 atgcagggca ttctcgacga catgcacaag gcagaggagt ggtgggatgg ccgctggcag
     9601 tgcagctttg ccgtcgatac cgggaatcat gacgccgcga cggcgatcct ccagtacgaa
     9661 tccggtgcac atgcggtcta tacccaaaac ttttacgctc ggcgcggggc tgcgaagcgc
     9721 ggcgcgacct tcatcgggta caagggaacc gtctcgttcg actgggtgac gagtgaggtg
     9781 gttgttcatc accatcacac cccacgggtt gagcgacacg tcctcggcgg tggggacggt
     9841 ggtcaccatg gtggcgaccg cgagcttgcg cacgacttcc tggcgattct caacggtaca
     9901 gggaggagcc gggcaacgct ggaggatggg ttgctcagcg cgcagctctg catgatggca
     9961 aagcggagct gccagacgag tggttttgag acttaccacc ccctgaggag taccgcaacc
    10021 gttcggtagt tgcatggacg gtcgggtatt ctctcgttgc ttacccgtgc tgtcgctctg
    10081 ggaattcctg agaacatggg tcctccgtac attcggagga aaccgtcgac gggccctgtg
    10141 cgtaggcatg aacgggattg cgtgagcgct cacgccggta gcggcatggt atcttcatgc
    10201 cgagaacttc ctgatatccg cagttttccc taaccaaggc aggtaacact tgaatttcga
    10261 attgaagcgt ccgggccgat cgcgattcct tcagcgaccg atgaaccggc ggaagttgct
    10321 gcatgccagc gcgctgtcca ccgcaggcgg gctgttgttg ccaggcgcat ctggtgccca
    10381 agaaagtagc ctggatctcc caccatcgca gaacacctcg accccgtctg cgcccatcgg
    10441 cgcacggttc aacgaaggac tgctaccgga tcatcgcctg atcatgtatt acggatttcc
    10501 cgagaacccg aatatgggaa tccttggtga gtacccgccg gagcagctct tgccgcaatt
    10561 gctcgagcag aaggctgagt acgaggctgt catcaatgac cgcccgtgga aggctggttt
    10621 tgagctgatt gcatctgttg cgcagcgtga accgggccca gacggcaagt acatcgccga
    10681 taccgatggc aagtggctgg acatgtatac ccagttcacg gaagccaacg acatgttcct
    10741 ggtgctcgat gtgcagatgg ggctcaagac gccgaaggaa gactatgaag gactggagcg
    10801 gtggctgcgc tacgaccacg tccacctggc gatcgatccc gagttccatg tgcttccggg
    10861 cgagactccc ggtattgagc tcggatcaat agatgccgca gatgttgcgg aggcgcaggc
    10921 ctggctgttg cagcttgccg agaagtacgg cacatcgcgc aagatgttgc tgatccacca
    10981 gttcaacacc tactccgtat cgaacaagga ccaaattaag ccactcgagg gcgttgacct
    11041 tgtactcaat atggatggat gggggccacc atggcagaag ctggagacat gggaggtgat
    11101 catccagcag acgccgatcg aatataacgg ggttaaggtg ttctaccgtc aggatgagcc
    11161 gatcatgaca ccggccgagg tgatcgcgct ggaacctacg ccagacgtaa ttaactacca
    11221 gtaagtcgat tctcagagac gaatgcagtg taccccggtg gagctgtcca tcggggtaca
    11281 tcgtgcgacg gtgcttgggc gggatgcgtt tctcccattc gatggagtgt ggagcaagca
    11341 ctaccggagt gcatagttct tcaggggcga actccatgtt cgcccctacg atttggatgc
    11401 cttcagaggg taccggccga ttacttcgta gcgggcgccc tctcggctga ggtggcttcg
    11461 aacaagatgc acctcgcgca cgggaaaggg gactggtttc tcgtgcgtaa cctcaccgct
    11521 ggtcgctagc tcctcgaatc gggtgcgaat cgtgccaggg aggtcgcgca cgcggatggt
    11581 gcgggagtcg cgtacccggc cgagcgtgat gtgagggcgg aactccttct cgtccagctc
    11641 gacctcgaag gattgcagca acgatccgat gtcgttccgg atcgtatcca gccggtgggc
    11701 cgggccccag agccccagcc agagcacccg tggacgcttg atggacggga aggcaccgag
    11761 gtcggcggtt ctgaggtcga atgccgcatg tggcgcaatt gcttccccga gcgccaggcg
    11821 gagcagttcc gcgttctctg gagcggtatc gccaaggaaa tgcagcgtca gatgggcgtt
    11881 gtcaggatcc gtccagcgga ttggccagtc gttgccgcga aggtcggcga tgatccgtgc
    11941 gacgaccgct cggacttcat cgggtaacgg cacagcgata aagagacgcc acggggtgtc
    12001 ttcggattcc tcgggcggtc gctcccttac aaagcgctgg cggcgctgct tcggggtgcg
    12061 gcgttgtggc tgttggccgt cgttgttctc catggaggga gtatacggga gcgcgaataa
    12121 gacctcgctc gggtatggtt taccagatga tagtggcgtg acggaggaca ggtgcaatgg
    12181 cggtggcgta tctgggacct cggggcacga acagcgagga ggcggcgatt ctctatgctg
    12241 gaccggaggc tgagctcgag ccattttcgg ggatgccgga agtgatccag gcggtggaga
    12301 gcggagcagc ccgggcggga gtgctgccgg tagagaactc catcgaggga ccggtcagcg
    12361 tctcgctcga tctactgatc catacgtcga acctcaagct gtgtcatgag gtggttgtcc
    12421 cgattcgaca tgtgctcgtt gcgaagaaag gcgcacgacc tcagggcatc cgcagggtga
    12481 tgtcacatcc gcaggcgctc gcacaatgcc ggctcttcct ggagcgggaa ttcccgcagg
    12541 cggagcaggt cgcctggatg tcaactggcg gctcagttca ggaggtcgcc cgtggtgatg
    12601 acctcacggt cgcggccatc gggccggagc gcgcccaaca cctgtatggt ggcgaagtga
    12661 tcgcacgcga catccaggac gcacgtagca acctgacccg gttcatggtg cttgcccatc
    12721 acgatgcaga accgacgggt gacgacaaga ccttcatcgc cctgaagacg gacaaggatg
    12781 agccaggcag ccttttcagg atgctcgaac ccttcgcaaa ggccggcata caactcacga
    12841 atgtgattac ccgaccgacc aagggctggc tgggagagta cgtcttcttg ctggacttcc
    12901 aggggcaccg gaaggacccg gtggttgcga agttgctcga tgacgtccgt gaggtggcgg
    12961 atgaattgaa aatcattggc tcgtgcccgc gctttccggt tgagcaatac cgggacctgt
    13021 tagcggggta gggcggggca acgccgcatc cctgaaacac gggagaccac aagggtctcc
    13081 aatgcagccg aattaaagcc aacatatgag agatacagaa tgaccctcta cattgatgaa
    13141 tcgttgatga ccgcacaagt caggattcca ctggtcgagg gctgggtgga aaccgatgtg
    13201 cctttcgaga tccggaggaa cctccaggcc gcggatatcg gatcgagcga tgtagcgctg
    13261 attgccggac cggaggcggc ctttctgggg gagacgcatt tcatcgagcc agagatcgcc
    13321 gtggtcgcgg aggcgatgag cgcgatcgtg atgcgcacac cagtacggcc ggatggggtg
    13381 gaggagacac cgatccggat gctcggtact ggtccgaccg ccgagctgct gatccgggcc
    13441 ctgcttcgtc cctattttgg catcaccgca tccaccttcg tgagggatga cgatgatccc
    13501 tcggcggccg atgcccaggt cgtgatcctg gagggcctgc tggcgctgaa cgagcccgag
    13561 gcgggctttc aggaggatct ggcaaaggcc tggtacatcc ttaccaacgc cgctgtggtg
    13621 catcatgtga cggttatcgg agtggaggca gaagcgcggg gcgcgacgac cgagattgac
    13681 ctcctgaaac gggccgtcca ggccggtgtt gagcggcggc gcgatgttcg gcgggagatg
    13741 gtgggggatg aggagatcga tcgggatcgg ctggcggagc tgacgaaccg gattcgcttt
    13801 gagctttcca ccgcggatcg acagtcgcta cgcaacctgc tggcacgagg ctcctggggc
    13861 acgtcctacc tgcgggcact gccggcattc cgggatgagc tgacatcgcc ggagtagtcc
    13921 ggcccacacg cctaggttcc ggacggggta tcgatgcgac cggtctcgat ctggtaatcc
    13981 agctctgaac cgatccacga ggagccatcc tcgtaccatt cctttttcca gatcggcacg
    14041 atctccttga tccgggcaat ggcgaacgac gaggcgtcgt atgcgttctc ccgatgcgga
    14101 gatgcgcacg cgatgacaac gctggcctcc cccggttcga ggtgcccgat gcgatgggag
    14161 atcgcgatcc ttacctcggg ccattggtcc gctacttcgg tcccgacacg ggccagcatc
    14221 ttctcggccg ctgacgggta ggcttcgtaa tcgagcgcgg tcaccgtaag accgcgcgca
    14281 ttgtcgcgca ccgtgccgat aaaggtgaca atggcgccgg agtccggtcc agcgacgaga
    14341 gattcgacac tgcgtgggtc cagcacattc tccgtgacag tgaagagctg cggcaaatga
    14401 tccccaccgc tcaccggggg aatcagcgcc acctcatctc cctcctgaag gggatggtcc
    14461 gggtcgacat actcctcgtt caccatcacc atcacggcac gctccagtcc cgcgaggcgt
    14521 ggagcctccc ggatcgcgag gctgaaaatc tccccggcgg tcgtaccatc ggggacataa
    14581 agcgcttcgt cggctttgcc aagatgctcg cgcatgaccg cgaaatagcg aacggtgacg
    14641 ttcatcgaac cccctcagtg ttttccattc agcatgggcg caaggagacc tcgccggagg
    14701 cagaagcgac tcggcgaggt ccctatcatc agcgatgagc agattcggga ccaggctagc
    14761 cctggtcgag gacggtcttc aggcgatcgc gcaggcgact ggcaaactga ttgttcacct
    14821 gttcgagttc cctgaagagt tcggacacct cgctctcgcc cgcttcttcc gcgtccttcg
    14881 cataccggtt caggacatcc tcggcctgaa ggagattgct gagcgtcgta acgatgttgt
    14941 attcgacgtc ggagacgccg aagcgccctt ccttgtgcgt catctctatc ctccctggat
    15001 acggaacgta catccattca ttcctggagc taccgcacga tgtctgccat agcccggagc
    15061 aggaagaatt agaaccgttg actgagcccc acacaccagc cgcttgatag acgccccatc
    15121 ggcgacacgg atccggtggt gcccggttga ccgtggctac aggtacgtct cgaaccagcc
    15181 caaggtgcgt tcaagccttt caacacggtg cttcggctta ccgttgcgac caagaccgtg
    15241 gctctcgttc gggaaacgga ctaactcgac ggtacggtcc agcttcttga gggcgataaa
    15301 gagttgctcg gcctgctcca tgggacagcg gtagtcctcc tcgctatgga caatcagcag
    15361 cggggcggtg atgttctcga cgtactttag cggcgagagt ttccagtagg tcttgaagtt
    15421 gtcccacggc gttccaagct ccggatcgag gctgacgagc gcgatgtcat cggtgccgta
    15481 catgctgatc atgttcgaga tcgatcggtc tgtgacggcc gtcctgaagc gatccgtatg
    15541 cccgagcagc cagttggtgc tgaatccgcc gtaggagcca cccgtcaccc ccagccgctc
    15601 cgggtcaagc ccacccagcg agagcacgtg atcgaccgca gccatgaagt ccggcatgtc
    15661 tgcaatgccc cacgcgccgt tcacggcctg cgcaaacgca tcggtacggc cggtgctgcc
    15721 tcggggattg atgtagacaa gggcgttccc ccgggcgacg tacatctgaa gctcatgcga
    15781 ccatgcgttg ctgaactgcg cgtgcgggcc accgtggata ttcaggatca acggcaccac
    15841 agcgccgtca gacgagttgc ctgctggcct gatgagccaa ccctggattg cctcgccatt
    15901 cgacttcacc cagaattcct ccggggtcgg gatcgcaacc tggcccagcc actcggcgtt
    15961 gaacgacgtc agcggcgttt cctcctcagc ggttacgcgg cggagttccg ccggggtagt
    16021 aggatcggtc acggaaatca ccagctcatc actagagatg tgatcgaagc cggtcacgtg
    16081 ccggtcaggc cctgtcagct gcatcggccg cttgccattg aggcggaagc gcatcaccac
    16141 cgtcgccccc cgatcggaga ggagcgcgtc gatcgcggag ccatccgaca gccaacggag
    16201 cggtgctccg gctggaccat tcggcccgct catcgagctg tcgccgacgt accggtcgag
    16261 ctccttcccg actcgttccg gcttcccgcc agcggcgggg atgatgtaaa gccaggagtt
    16321 cacctcatgc agctctacat cagcattgcc cgtgatggcg atcgaagttc catcgagcga
    16381 ccagacgggg gcacgccacg atccctggcc atcggtcagc ttcctgggct cgccgccatt
    16441 tgggttgacg gtccagaggt cgttcttacg gctcttgtcc cagttgtcat cccgattggc
    16501 gacaaacgcg atctcgtgcc cggagggcga ccaggcagga gcggtgtggt tgaactggcc
    16561 ctcggtgagt ctggtcggct cctcttcggg ggtagagacg cgaacgacgt aaagctggga
    16621 aaccttgtcc tccaaaaacc cctcgccgtc gaacttgtag cgggcggagg tgatgacctt
    16681 gacgtcgctc tcgctctcgt cctccttctc ggggccgatg cgggaggtga agacgatgcg
    16741 gtcgctatcc ggcgcccagg aaaattcgcc gacttcgttc tcgcccctgg tgacctggat
    16801 cgcctcgccg ccggcggtag ggaggacaaa gatctgggtt ttcttctcat tccggttgga
    16861 cgtgaaggcg atccacgaac cgtcgggcga ccagcggggt gaactgtcgg tccactggcc
    16921 cgaggtcagt tgcgtcggtg catccccgtt gagtggaatg ttccacaacg cggtgcggta
    16981 ctcgtctgac tcgcggtcga tcgtcagcac cggagcgaca acacgggtac catccggaga
    17041 gacgcgtgga tcaccgacga gcgaccattt gtagagatcg tcgatcttta ggggccggcc
    17101 agtacgctcg ggagcggagg aggcatgctt gaccgcgctg gtgcgcttta cctccaggtc
    17161 gagaccccgg cgcatctgtt tgagcgaatc cctcttccct ttggccttca ccgccctggc
    17221 agctttcttg ccagttttcg attccttgtt tcccatcgtc aggtggtcct ttcactgcct
    17281 tgcagttgcc caaggctgat tggtgtcagt tcagggtttg gtaagggaca gtctggcgct
    17341 gacccctaca gagctggtat cggcgcacca agcataaggg cgtcgatggt ttccccggcg
    17401 gcgtagtcgg tttcgcgtgg ggcgatgacc agaagtgcgt tcgcgcccag aaacgatgcc
    17461 aggcgggatg atcgctgcag accggtgctg cgggcgatga gcttaccgtt ccgttcggcg
    17521 gagacgaccc cccgctggta ctcgatccgg tctgacggtg atgttccttc aaccagcgtg
    17581 acgcggacga ccggtcgatc gacctctgtt tcgccgatca tctggcgaat cgccgtccga
    17641 atgaagagct cgaacgttac cagcgaagag acgggattgc cgggcaggcc aaagatcaat
    17701 gtgtcaccgt gggtcgcgaa gttgagtggc tttccaggct tcatgaagag ccggcggaag
    17761 tggacatgga catcaccggc gtcgaagagg atggccttga tgaggtcgcg ctcgcccatg
    17821 gagacgcctc cagaggtgat gatgatgtca tcggtcgcca tgcgctcccg caggagggct
    17881 tcaagttggt cgagcttgtc gggagccttg ccagcgaacg tgatctcgac tggttctgcc
    17941 aggagcgcgg cgatgagact gaagcggttg gaatcgcgaa tctggccagg gccaattggc
    18001 tgatccggct ccaccagctc gtcgccggtg gagataacgc tcacccgggg acggcgtgcg
    18061 accggaaccg ggctgatgcc catactggct accaggccga tctctgcggg gccaaggcgg
    18121 gtgcccgcct ggagaacgag cgttcccctt gcaacgtcag accccacagg gcggatgttt
    18181 tccccaattg ctacatcttc ctgatggata accacatggt cctccatcag ttccgtggcc
    18241 tcaactggga ccacggcgtc tgcgccgggc ggtaagggag caccagtcat gatcctgacg
    18301 gcataaccct ccgtgacctc tgcatcaatc gtgtcgccgg cattctgggt gccgatgatc
    18361 tctcgccagg gagagtgatc ctgagagaag acggcatagc cgtccatcgt cgatgccggg
    18421 aagggcgggt gatcctccgg cgcggtgatg tcctgg
//
LOCUS       DASYWM010000281         3839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_669879, whole genome shotgun sequence.
ACCESSION   DASYWM010000281 DASYWM010000000
VERSION     DASYWM010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669879"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1179)
                     /locus_tag="VGR29_11470"
     CDS             complement(241..1179)
                     /locus_tag="VGR29_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV2074246.1"
                     /translation="MKQTRQPALLVLLITTIVAIGWGGTTLNPVARGSDPGSRDTTGL
                     SVALPTTYPQEPARTASPEPTDAPETTPNPTVVPTEPLTPASEPAETPRPPVPENTFD
                     QSYIVERGESGRMEVALTFDAGEGAGFTSEILDFLAANNIKGSFGITGEWAREHPKLV
                     QRIVDDGHHLFNHTDSHRSWTGVSTTGEPLSPEDRIAELERAERTVQDETGYSMKPYF
                     RPPYGDYDVEGLELLKEQGYEYTLWWTCDSLGWNGATAAEVVERCGPDADGGGPGAIV
                     LMHVAQENDYLALDELVEAYRAAGYDFVTMEQMIQP"
     gene            complement(1260..1571)
                     /locus_tag="VGR29_11475"
     CDS             complement(1260..1571)
                     /locus_tag="VGR29_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV2074247.1"
                     /translation="MAAEFNKDDVFDGLKNVYDPEIGINIVDLGLVYDADIDETGDVL
                     VTMTLTSLGCPLGPVIMQEVDNALKDLPGIGDTDVKLVWSPPWTPDMMSEEAKDELGI
                     W"
     gene            complement(1788..2528)
                     /locus_tag="VGR29_11480"
     CDS             complement(1788..2528)
                     /locus_tag="VGR29_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV2074248.1"
                     /translation="MFVVGMLIGFIGAGGAGVMVSLLTIAFGLPIHEAVGTALAAMLF
                     TTISGSISHFREGNVAIAPGVVIGVAGMTGAVFGADIGQDIPEYILQPMAGFALWFLA
                     FLVWLRIRLRGKLKEIAEDATLEPLKPGQFAGGAGVGVLGGMASAFFGVGMAPFVQLG
                     LLTVMRLPLTKAIGTTMLALSFISLSGSIALASHGDVSAIHLVGVTIGMTAGSYAGAK
                     FTRRAPADLLRMAIVATPFLAGVPLIFF"
     gene            complement(2555..2923)
                     /locus_tag="VGR29_11485"
     CDS             complement(2555..2923)
                     /locus_tag="VGR29_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074249.1"
                     /translation="MSFNTYTPPGWWDRTRLLRYAFALGLVFGVGIGWFFHNIISMIF
                     QFGLVVLLLLPLATVAFMWWRSNRERNRMQSSTTVMRWGNGQFPPYGPDMAADQFGRL
                     RFDRDEIIDADDASYQEPRR"
     gene            3210..>3839
                     /locus_tag="VGR29_11490"
     CDS             3210..>3839
                     /locus_tag="VGR29_11490"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HEV2074250.1"
                     /translation="MKIAYFDPFSGASGDMILGALIDAGLRLTDLTMMLSKLELSGYR
                     IGAERVGQHGVHGTRVTVSVFEDVYSRTWGDIRQLIEESGLELAVKTAALQIFERLAV
                     AEAKIHNTTPDEVHFHEVGGADAIVDICGACIGLALMQIDAVMCGPLQLGHGFATSAH
                     GTIPVPAPATLELLAEAKAPAARPTPLIAETPAELLTPTGAAILTTLATF"
BASE COUNT          779 a         1183 c         1125 g          752 t
ORIGIN      
        1 ggtggacata cgctgcatcg atcttcaaag gaagaattcc tggcggctgc ggtgccattt
       61 ctactggcac atgcctgacg tcgtcttctg cccagggagc tcccgccatc tggtgcttgc
      121 aggcacatcc ataggcgcga gtcaatgcca atgtccggtg tggacggact taagctttta
      181 agcttcgcct tcattgccgg gcaagtcgtg ttgagccact cgtgctgggc accagaagga
      241 ctatggctgg atcatctgct ccatggtcac gaagtcgtac cccgcggccc ggtatgcctc
      301 caccagctcg tcgagcgcga gatagtcatt ctcctgggcg acgtgcatca gcacgatcgc
      361 tccgggtcca ccgccgtcgg cgtctggacc gcaccgttca acaacctccg ctgccgttgc
      421 accgttccac cccagactgt cacaggtcca ccaaagcgtg tactcgtagc cctgctcctt
      481 gagcagctcc agtccctcga catcgtagtc accgtacgga ggccggaaat agggcttcat
      541 cgagtatcct gtctcgtcct ggacggtcct ctcggccctc tcgagctcgg caatgcgatc
      601 ttccggcgag agtggctcgc cagtggtcga taccccggtc caggaacgat ggctgtcggt
      661 gtggttgaag agatgatgcc cgtcatcgac tatgcgctga acgagcttgg ggtgctcccg
      721 cgcccattcc cctgtgatac cgaaactccc cttgatgtta tttgccgcca ggaaatcgag
      781 gatctcgctc gtgaagccag ccccctcgcc tgcatcgaac gtgagcgcga cctccattcg
      841 gccggactcc ccccgttcca cgatgtagga ctggtcaaag gtattttcag gaacgggagg
      901 tcgcggcgtt tcagcgggct cggatgctgg tgtcagcggt tcagtaggaa caaccgttgg
      961 gttaggtgtt gtctccggag catctgtcgg ttccggcgat gctgttcgcg ctggctcctg
     1021 aggatacgtg gtcggcaatg cgaccgagag accagtcgta tccctggagc cgggatcact
     1081 accacgagct acagggttaa gcgtggtacc gccccatcca attgccacga tcgtcgtgat
     1141 cagcaggaca agcagggcag gttgtcgcgt ctgtttcatg gatcgggccc ctgccctgta
     1201 cgctacgaat ggcttttgcc ggaaatctgt ctacctaccg gatgttgcaa caacagcccc
     1261 taccagatgc ccagctcgtc cttggcttcc tcgctcatca tgtctggtgt ccagggcggg
     1321 ctccacacca gcttcacgtc cgtatcgccg atgcccggga gatccttcag ggcgttgtca
     1381 acttcctgca tgatgactgg tccgagcggg cacccgagcg acgtgagtgt catcgtgacc
     1441 agcacatcgc ctgtctcatc gatatccgcg tcgtagacga gcccgagatc aacgatgttg
     1501 atgccgattt ccgggtcata gacgttcttg agaccgtcga agacgtcgtc cttgttgaat
     1561 tccgctgcca tgagaagtgc aatcctccgt gagggtatat caatcgatcg ggcggatgcc
     1621 ccggcatcct gacgggtgcg cccgattagt tccattgtac cgggcgtggc gagtcccgcg
     1681 cgggagcagg tcagtatggg gccaatatgc tggtcgttga ggagcatcct gcatcctgca
     1741 cgcctcgctt gctctaccgg atcaaactcc gtcgttgtca accatgttta aaagaagatc
     1801 agcgggacgc ccgcaagaaa gggggtcgct acgatcgcca tccgaagcag gtccgcaggg
     1861 gcacgacggg tgaatttggc gcccgcgtag gaaccagccg tcataccaat cgtgacaccg
     1921 accagatgaa tggcagagac atccccgtgg ctggcaaggg cgatgcttcc gctgaggctg
     1981 atgaaggaga gggccagcat ggtcgtcccg atggccttgg tcaagggcag ccgcatcacg
     2041 gttagcaacc caagctggac gaagggtgcc atgccgacac cgaagaacgc cgatgccatg
     2101 cccccgagca cgcctacgcc ggccccacct gcgaattgcc ccggtttcag cggctcaagt
     2161 gtcgcatctt ccgcgatctc cttgagcttg cctcgtaatc ggatccggag ccataccagg
     2221 aacgccagga accagagcgc aaagcctgcc atgggctgga ggatgtactc ggggatatcc
     2281 tgcccaatat ccgcgccgaa gacagcgccg gtcatccctg ccacaccgat cacgacgccc
     2341 ggcgcgatcg cgacattccc ttcacggaaa tgcgagatcg agcctgagat tgtggtgaag
     2401 agcatggctg cgagggcagt gccgaccgct tcgtggatcg gaagcccgaa ggcaatcgta
     2461 aggagggaaa ccatcacgcc ggcgccacca gccccaatga agccaatgag catgccgaca
     2521 acgaacatga ccgccagaag tgagaaaagc agggtcagcg acgaggctcc tgatacgaag
     2581 catcgtcagc atcgatgatc tcatcccggt caaaccggag gcgaccaaac tggtccgctg
     2641 ccatatcagg gccgtagggc ggaaactgcc cattccccca gcgcatgacg gttgtggacg
     2701 actgcatccg gttgcgttcc cgattcgagc gccaccacat aaacgcaact gttgccagcg
     2761 gcaggagcaa caagacgacc aggccgaact ggaagatcat agagatgatg ttatgaaaga
     2821 accatccaat cccgaccccg aaaacaaggc caagggcaaa ggcgtaacgg agcaggcggg
     2881 tgcgatccca ccatcccggg ggggtgtagg tattgaagct catgtgggca cctccgtaga
     2941 caagacccct ggtcgcaatc agaatgcatc aaacatcggc gacccacagg tgtgctgctg
     3001 tattagtgta tccgaacacc ggccctcccg gtgatggggg ttatccggaa ttccatgcgg
     3061 gaaatccgag ggagccgcga gaacagcgac cggggatctt ccctttccta cgacgccgcg
     3121 actgcgcctc ttcacgatga tttgaagaac gaacgacggc gtatcaacgt ttcgctgaac
     3181 gccttctccg atggagtgtc ctcttcataa tgaagattgc ctacttcgat ccattttccg
     3241 gtgccagcgg cgacatgatc ctcggcgcac tgattgacgc cggactccgt ctcaccgacc
     3301 tcacgatgat gttgagcaag ctcgagctca gtggctatcg catcggcgcg gaacgcgtcg
     3361 gtcagcatgg tgtccatggc acccgcgtga cagtctccgt cttcgaggat gtctattccc
     3421 gtacttgggg agatatccgt cagctgattg aggagtcggg gctggaactc gctgtgaaaa
     3481 cggccgcgct tcaaatcttc gaacgtctgg cggttgccga ggcgaaaatt cacaacacca
     3541 caccggatga ggtgcatttt catgaggtgg gcggcgccga cgcgatcgtc gatatctgcg
     3601 gtgcctgtat tgggctggcc ctgatgcaga tcgacgcggt aatgtgcggg ccgctccagc
     3661 tcggtcacgg tttcgccacc agcgcacacg gcacgattcc ggtccccgct ccggccacgc
     3721 tggagctcct tgccgaggcg aaggcccccg ccgcacggcc gacgccgctc atcgccgaga
     3781 ccccggcgga gcttctcacg ccgaccggcg cggcaatcct cacgaccctg gccaccttt
//
LOCUS       DASYWM010000282        12391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_332651, whole genome shotgun sequence.
ACCESSION   DASYWM010000282 DASYWM010000000
VERSION     DASYWM010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12391
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332651"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1848)
                     /locus_tag="VGR29_11495"
     CDS             complement(136..1848)
                     /locus_tag="VGR29_11495"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV2074251.1"
                     /translation="MPGRRLSWLGYLIHNPSDPGMRLLGKRMNRRAAASTSAGGLLAL
                     ATLRTNAQEAPPIEPIGADEPIPPDTSSAPDAPVEILPESTLSPDEVPAGENVGGSTS
                     GEEIYFAATGHNLGTPFLDTWSAAGGFEVLGAPLSEARYIADVVETHQTFETLTLTYD
                     PDLEYDWRIQALPLDQKMIDRISSAESRKKVTTGSGTGTLYPETGHSLSGSFHAFWEQ
                     HGGVKLFGLPVTEPFSSSAGTSQVFERVVMDRAANGTVMLRRIGPEWVRDNNLEGDEA
                     FVAAPPNNGNTTMVRADGGLRLRGGPSEDAEIRVILPDNAEFIAVAGAEGEWVPGYVD
                     GYSGWVASAFLVAPTSIAVIDSSEWDTSVWQGAALGETNVRAEPTTASAEVRVLAHGE
                     PVVVVDWVRGEEVVENSFIWAQLDDGSYVFARNVGRAAPVQPPPVPGDAPWEGKWIDV
                     HLTQQLMVAYEGRTPVRTVVTTTGMPGWETPTGWFAINTRVENETMDSGAIGAEYFYK
                     LEDVLYTQYFSDRGHALHYAWWRTPETIGRPGSHGCLNMLLADSKFFWDWANIGTPVI
                     VRRV"
     gene            2004..2921
                     /locus_tag="VGR29_11500"
     CDS             2004..2921
                     /locus_tag="VGR29_11500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2074252.1"
                     /translation="MKSELHRFIDYPRGDAPQLGVVFPQTAFFGGSDELRTFATSAES
                     LGFRHVLAYDHVLGASKVTRPDWSGPYSSDDPFVEPFLLFSYMSAVAPSLEFTTGVLI
                     LPQRQTVLVAKQAATLDLLSGGTFRLGVGIGWNRVEYDGLNEDFSNRARRFSEQIDLI
                     RQLTSHEVVDYSGTWHRIDHAGIRPLGIQRPVPVWIGGSAEAAIRRSARIADGFFVNG
                     TVIERMEPALRVLRDELDKQERSESTYGIEVRVSVAENNHDAWKRDFAHWREANVSHI
                     SIVTLGANFDEFPQHLERFAEARRVWDEM"
     gene            complement(3071..3769)
                     /locus_tag="VGR29_11505"
     CDS             complement(3071..3769)
                     /locus_tag="VGR29_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEV2074253.1"
                     /translation="MLMYIVFLIPGLLFMLWAQNKVKSNYKKYSQVRNTAGVTGAQAA
                     RRVLDNAGLYDVQIEAVQGELSDHYDPRSRVLRLSQGVYGVPSIAAIGIAAHEAGHAI
                     QHAKAYAPLQVRTAMVPLVSLGSNLGYMVLFAGLLMASQNIAWIGVGLFSLATLFALA
                     TLPVEFDATKRAKAALVQIGLVDGGVRGGDESKGVANVLDAAAWTYIAGFASSVLTLL
                     YYVMMVSGMGGRRE"
     gene            3990..5075
                     /locus_tag="VGR29_11510"
     CDS             3990..5075
                     /locus_tag="VGR29_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV2074254.1"
                     /translation="MQPSLAHRQNDQEAIERHRLFGLLEAEIDDALTTLSGTAPLMMT
                     MARYHLGLTDSSGEPTTPEIRQAVQGKRIRPLLAMYSAAAVGGRVEEAAPVAAGIELL
                     HNFTLIHDDIQDRSPNRRYRPTVWRIWGNAQAINAGDALFAAAQLALLRTNGTTASPS
                     LVVRLIEAFNTTTIDIVRGQVHDLENEGRPNVTPEDYLTMIAGKTAAIVRYSAWAGAL
                     VGGAPDEIADKLGQVGEAIGMGFQLRDDMLGIWSPAAVTGKDAADDIRRRKQSLPILI
                     LRARASEEDTTRLNALFSQEEIGPEGVLEVLALLTKYDVEETMTVQVGDAHTRAMTIL
                     NEALPNQANAAVDSLKTLVTQLSVRIS"
     gene            5103..5807
                     /locus_tag="VGR29_11515"
     CDS             5103..5807
                     /locus_tag="VGR29_11515"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2074255.1"
                     /translation="MDQDSLDGALGPRLNWTPALPPNGAALAGHSVSLQHMDAEAHAA
                     ALYGASHGEGSDPLLWLYMGSGPFDSLEEYRGSVERNSISTDPLFYTIIPEGEEAAGQ
                     ASYLRIDPANGAIEIGHIWFGSSMQRSRAATEAIFLLAKHAFDELGYRRLEWKCNARN
                     ARSRSAAERLGFTYEGTFRNAVVVRDRNRDTAWFSITKEEWAPIRTAFETWLSDDNFD
                     ADGRQRRSLSAIREAP"
     gene            5804..6502
                     /locus_tag="VGR29_11520"
     CDS             5804..6502
                     /locus_tag="VGR29_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEV2074256.1"
                     /translation="MSSFRPRRRADDEPAAAPKPGSITDIQPQRRDNARVSVYIDGMF
                     AFGLHIDLQLEHYLKKGDVLDEEKIALLLKQDAVKKAISTSLNLIAYRPRSAGELASK
                     LRERGYDAETIDEAIGRMRELNYLDDREFADRWVESRQMHRPRSTRMLRQELQQKGVD
                     RQIIESTIEGAGIDEFGDALALGQKKVESFRALEPQVRDRRLSGFLARRGYSYDVIRR
                     VLEALETGETVDGQ"
     gene            6793..7434
                     /locus_tag="VGR29_11525"
     CDS             6793..7434
                     /locus_tag="VGR29_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2074257.1"
                     /translation="MAEIRWLGHNCFRIRAREATVLMDPVGKKTGFTMARQTADIVTL
                     SHQHAGHTNLEAVKPEYQVIDGPGEYELHGVFITGIRSYHDRNKGTEGGYNTIYRVEF
                     DGLKFGHLGDLGHPLNDDQIESLSSVDILFVPAGGGPLLPPSEMAEVVGGISPRMIIP
                     MQYRAGKGDQSRDPVEPFCKHLGAEVPQPLEKLVVKASDLADQMQLVLLTPDG"
     gene            7505..9196
                     /locus_tag="VGR29_11530"
     CDS             7505..9196
                     /locus_tag="VGR29_11530"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV2074258.1"
                     /translation="MPKYVFVTGGVVSSVGKGITTASLGRLIKNRGASVSIMKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSRASNVTSGQLYSSVIAKE
                     RRGDYLGGTIQVIPHITNEIKSRIRLASRQHDSDVVIVEVGGTVGDIEGQSYLEAIRQ
                     MRRELGRGNSLSIHVTLLPEVGATGELKTKPTQMSVRELRGMGIQPDVIICRADREMP
                     GDVRDKIALFCDVEQDAVIGMPTASSIYEVPLILEDAGLGRYVAKAFGLPQEPNLREW
                     TNMVEHIHEPRKSLRIALIGKYVELHDAYLSVYESLMHAGLANDVNIEVDWINSETVT
                     QEELERELRRVSGVLVPGGFGPRGIEGKVSGVTFAREHNIPYLGLCYGLHMAVIEIAR
                     NLCGLEGANSTEIDANTPHPVIDLMPDQKNVDMGGTMRLGLWECRLQPGTKAADAYGA
                     PSVHERHRHRFEVNNAYRDQLEGAGFTVSGASPNGQLAEIMELRDHPFFVGVQFHPEF
                     RSRPNRPHPLFVDFISAAKEALPEGAQRLLPLEKELLPDSKLMIEVRAGQEMATH"
     gene            complement(9303..12059)
                     /locus_tag="VGR29_11535"
     CDS             complement(9303..12059)
                     /locus_tag="VGR29_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2074259.1"
                     /translation="MAEAARLKGVSYHTVSRAVRKGRLPVERLGRMALISARDLHAWR
                     PMKDRAPRKYRSEQAEGHEPGSDGSLLTPDHLTLARRLAALYEGIHSVASDGTIEEVA
                     QIATEQVAEALYLERMSLWLVDAEVNRGTRLAVVREPLSNLPDTVALDAFPIFSALTE
                     EPSARITADPLQDVPLEAGYTFPSGPLLVVPLLRKNRIVGVMFGDRYGDGIDFTEEQL
                     ALADGVASQVAIALDNTRLRQNERRRRLQMEAILDELANAVSAFDEQGFLTLSNSSER
                     ALFDLSEEESRLGQHATEYLRTKFRHGLDATETDINDSPVMQVLHGERPETLDQVITR
                     NNGETLLLRTSTRPIIAHDKIVGAISVSRDVTPEHRAEIRDRQQLQQLERAAHRSQAI
                     ADLVVEINTGRDRQTVVDTALRRLTQELDADCGAIMLHGEDDHFYLSACRGVRPELFP
                     SWPMDILASPVVAGAFTRYRPLVVPLNQLRVPEPMASELGDEAVAMIIPMQMHRQRIG
                     VAFVGFRKQPMLDDSDLAFGAIWGRQCAQAIDKVMLVGQLEYAHGRLMAIIDQLPQAV
                     LILDEPTGVVTVANQAADRLWGRSLEDGQIHPESLPVVDADGIPLVGEDHPLLRPLQT
                     HKEVIGQPLRVVQDDGTQVEVVANHAPIFDGRGVIIGSVGLLQDRSDFKPLDRAKDEF
                     ISVVAHELRNPLTSLRGNLQLMQRRIRKRADEHSDDELERLDSVITQVDRVADLVSRM
                     LDVSRVDLGSLDIAVAETDASMLARLAVTEAKGLNPDRPFVLEGPDVLPVVWDAARVQ
                     QVLGNLMQNAARYAPDGPVEVNLCETGTDRVKITVRDYGPGVPAKIQKRLFKQYYRFD
                     DGQDDGGTATNGSHGLGIGLYISARLAKAHGGSLTVDDADGGGAIFTLELPRDASRHD
                     TAEE"
BASE COUNT         2526 a         3855 c         3594 g         2416 t
ORIGIN      
        1 gcactatgcc atgttcccag tagggccacc aatgaacgta cagaactttc tgtcaataca
       61 gcgatgccca tgccctagag gatcaagcct ccaggcatat ggccagggaa tacgacgatc
      121 tccgtgccat gcctgctaca cccgccgcac gattacaggc gtgccgatgt ttgcccaatc
      181 ccaaaagaat ttggaatcgg cgagcaacat gttgagacac ccgtggctgc caggtcggcc
      241 aatggtctcc ggcgttctcc accacgcata atgcaatgcg tggccgcggt cggagaaata
      301 ctgcgtatag agcacatcct caagcttgta aaaatactcg gcgccaattg ccccgctatc
      361 catggtctcg ttctcgaccc gggtattgat tgcgaaccac ccggttggcg tctcccaacc
      421 aggcataccg gtggttgtca ccacggttcg gacgggggta cggccctcat acgccaccat
      481 aagctgctgg gtcagatgga catcgatcca cttgccctcc catggtgcat cgcccggaac
      541 cggtggcggc tggacaggag cggcccggcc cacgttgcgg gcaaagacgt agctgccgtc
      601 atccaactgt gcccagatga aggagttctc aacgacctct tcaccacgta cccagtcaac
      661 gacgacgacc ggctctccgt gcgccagcac tcgaacctcc gcactggccg tcgtgggttc
      721 cgcccggaca ttggtttcgc cgagagcagc cccctgccag actgatgtat cccactctga
      781 cgaatcaatc acagcgatag atgtcggcgc gacaaggaac gcagacgcca cccatccgga
      841 ataaccatcg acatagccgg gcacccactc gccctcggca ccggctacgg cgatgaactc
      901 cgcattgtcc ggaagaatga cgcggatctc cgcatcctcg ctgggtccac cacggaggcg
      961 aagcccaccg tcagcccgca ccatggtggt attgccattg ttcgggggag ccgcgacaaa
     1021 cgcctcgtca ccctccaggt tgttgtctct cacccattcc ggaccgatcc gccgaagcat
     1081 caccgtgcca tttgcagccc ggtccatcac cacgcgctca aatacctggg atgtgccagc
     1141 gctgctggag aacggctcgg tcaccggcaa gccaaagagc ttgactccgc catgctgctc
     1201 ccagaaggcg tggaaggatc cggagagcga atgccctgtt tccgggtaca gggtcccggt
     1261 cccactcccg gttgtcacct tcttgcggga ctccgccgat gagatccgat cgatcatctt
     1321 ctgatcgagt ggcagtgcct ggatgcgcca gtcgtactcc agatcgggat cgtaggtgag
     1381 cgtcagcgtt tcgaacgtct gatgggtttc caccacgtca gcgatgtacc gtgcctcgga
     1441 aagtggtgcc ccgagcacct caaatccgcc agcggcactc cacgtgtcca ggaagggcgt
     1501 gcccaggtta tggcctgtcg ctgcgaaata gatctcctca cccgatgtcg agccaccgac
     1561 gttttcgcca gcaggcacct catcggggga cagcgtggat tcgggcagga tctcaacggg
     1621 cgcatcaggt gccgaggacg tatccggtgg aattggctcg tcagcgccga ttggctcaat
     1681 cgggggcgct tcctgagcat tcgtccgcaa ggttgccagt gcaaggaggc caccagccga
     1741 tgtcgatgcc gcggcacgcc gattcatgcg cttgcccagc aaccgcatgc cggggtctga
     1801 tgggttgtga ataagatatc caagccatga gagacggcgt cctggcacga atgtactcct
     1861 ggtgagccgg taacctgcaa tgaaagggtt caaccggttt cataagtatg caacgataca
     1921 gagaacggac cttcgcaccg gtgtgcggct tcctgccgtt cacctccata aacgtacaga
     1981 tgcgcaggaa ggtgtcatca tccatgaaat cggaactgca tcgcttcatc gattacccgc
     2041 gaggtgacgc gcctcaactt ggtgtcgtct ttccacagac agcctttttc ggcgggtcgg
     2101 atgaactccg caccttcgcg acatcggccg aaagcctcgg ctttcggcat gtcctcgcct
     2161 atgaccacgt cctgggtgcg tcaaaagtca cccgccctga ttggagcgga ccctactcga
     2221 gcgacgaccc gttcgtggag cccttcctgc tcttttcgta catgtctgcc gtcgccccgt
     2281 ccctggaatt caccaccggt gtgctgattc ttccgcaacg ccaaacggtg ctcgtcgcga
     2341 agcaggcggc caccctcgat ctcctttccg gagggacctt tcgccttgga gtcggcatcg
     2401 gctggaaccg tgttgaatac gatggactca acgaggattt ctccaaccgg gccaggaggt
     2461 ttagcgagca gattgacctc atccgccagc tcaccagtca cgaggtcgtt gactactccg
     2521 gtacctggca ccggattgac cacgcgggca tccgaccact tggtatccag cgacctgttc
     2581 ctgtctggat cggtggcagc gcagaggcag cgattcgccg ctcggcccga attgccgacg
     2641 gattcttcgt taatggcacc gtaatcgaac gcatggagcc agcgctcagg gttctccggg
     2701 acgaactcga caagcaggag cgctccgaat ccacctacgg tatcgaggtc cgggtgtccg
     2761 tcgcggagaa caaccacgat gcgtggaagc gcgactttgc ccactggcga gaagccaacg
     2821 tcagtcacat ttccattgtc acgctcggcg ccaacttcga cgagttcccc cagcaccttg
     2881 agcgatttgc cgaggccagg cgcgtctggg atgagatgta ggcactcact cgagcggctc
     2941 ttccctgcgc tgcaatggtc gtcataccgg gtcaggcatc ggtgcacagg ctacgatcac
     3001 cttcaataca agacgccctc cccagtcgaa ctggggaggg cgtcgggttc gagcgcgatg
     3061 gtagatgccg ttactcgcgg cggccaccca tgcccgagac catcatgacg taatacagca
     3121 gtgtcaggac ggacgaggcg aatccggcga tgtaggtcca ggcagcagca tcaaggacgt
     3181 ttgccacgcc cttgctctca tctccacctc gaacaccacc atcaaccagc ccgatctgca
     3241 ccagggccgc cttggcgcgc tttgtggcat cgaactcaac aggcagcgtt gccagcgcaa
     3301 agagtgtggc aagactgaag agtccgactc cgatccacgc gatgttctgg ctcgccataa
     3361 ggagaccagc gaacagcacc atataaccga gattggaacc aaggcttacc agcggaacca
     3421 ttgcagtccg cacctggagt ggtgcgtagg cctttgcatg ctggattgcg tgcccggcct
     3481 catgggccgc gatgccaatc gcggcaatcg acggcactcc gtacacgccc tgggacaggc
     3541 gcagaacccg gctgcgagga tcgtagtgat ctgataattc cccctggacc gcttcgatct
     3601 gcacatcgta caaaccggcg ttatccagga ctcgccgggc cgcctgggca ccggtcacgc
     3661 ccgcggtatt gcgtacctgc gagtacttct tgtagttgct cttcaccttg ttctgggccc
     3721 acagcatgaa gagcaggcca gggatgagga agacgatata catcaacatg gtggatcgtg
     3781 tccttatact ttacgcagat gcccggcccc gcgccgtatc cggtggcagt caaggcaacc
     3841 tgcgaagggt gagttctgga ctcgccccgt acgtctattt tacgacattc cgcgctcgtc
     3901 tgttgggatc tctcgccgga tatggcgaca tggtacgcaa catcgcgtgt ggttaaatcc
     3961 gcgtacggat gatggagaac cgcttgaccg tgcagccctc actcgcgcat cgccagaacg
     4021 atcaggaagc catcgagcgc catcgactct ttggccttct tgaggccgag atcgacgacg
     4081 cactaaccac actctccggt acagcgccgc tgatgatgac catggcacgg taccacctcg
     4141 gcctcactga ttcatccggt gagccaacga ctcccgagat tcgccaggct gttcagggca
     4201 aacggatacg cccgctcctg gcgatgtata gcgccgcggc cgttggcggc agggtagagg
     4261 aagcagcccc cgtcgccgcc gggattgagc tgctgcacaa cttcacgctg attcatgacg
     4321 acatccagga tcgcagcccc aatcgtcgct accgtccgac cgtctggcgc atctggggca
     4381 atgctcaggc catcaatgca ggcgacgcgc tctttgctgc tgcccagctt gcgttactgc
     4441 ggacgaacgg aacaacggcc tcaccttcat tggtcgtgcg actcatcgaa gccttcaata
     4501 caacaaccat cgatattgtc cgtgggcagg tccatgatct ggagaacgag ggccgcccga
     4561 atgtgacgcc agaggattac ctgacgatga ttgccggtaa aacggccgcc atcgtccggt
     4621 actcagcctg ggcgggagcc cttgtgggag gcgctccgga tgagatcgct gacaagctcg
     4681 gtcaggttgg agaagcgatc ggcatgggct tccagctccg cgacgacatg ctgggcatct
     4741 ggtcaccggc agcggtgact ggcaaggacg ccgcagacga catccggcgt cgcaaacagt
     4801 cgcttccgat cctgatcctt cgcgcacgcg cctcggagga ggatacgacc cgattgaatg
     4861 cactgttctc tcaagaggaa atcggcccag agggagtgct ggaagtgctg gcgctgctga
     4921 ccaaatacga tgtcgaggaa acgatgacgg tccaggtcgg ggacgcccac actcgtgcaa
     4981 tgacgattct caacgaggca ctcccaaacc aggcgaatgc cgccgtcgac agcttgaaga
     5041 cgctcgtgac ccaattgagt gtgcgcatat cgtaggcaac gtccgggagg aggtgcattg
     5101 tcatggacca ggattcgctc gatggtgcgc tcgggccgcg cctgaactgg accccggccc
     5161 tcccgccgaa cggtgcagcg ctcgccggcc actccgtgtc gctgcagcat atggatgccg
     5221 aagctcatgc tgccgccctc tatggtgcat cccacgggga gggcagtgat ccccttctat
     5281 ggctctacat gggctcaggt ccatttgaca gccttgagga gtatcgcggc tccgttgaga
     5341 ggaacagcat atccaccgat ccgctctttt acaccatcat ccccgagggt gaagaggctg
     5401 ctggacaggc gtcatatctc cggatcgacc ctgctaatgg cgccatcgag atcggccaca
     5461 tctggttcgg atcgtccatg cagcgctcac gagccgccac cgaagcaatc ttcctgctcg
     5521 cgaaacacgc attcgacgag ctgggatatc gccgtctcga atggaaatgc aacgcgcgca
     5581 acgccaggtc ccggagtgcg gccgagcgcc taggatttac ctacgagggc actttccgca
     5641 atgccgtcgt cgttcgcgac cgtaaccggg atactgcctg gttctcgatc acgaaagagg
     5701 agtgggcgcc gatccggacc gcctttgaaa cttggctatc ggacgacaat ttcgatgcgg
     5761 atggccgaca gcgccgctcg ctctcggcga tccgggaggc gccatgagtt ccttccgacc
     5821 gcgccgtcgc gccgacgacg agcccgcggc cgcaccaaaa cctggcagca tcaccgatat
     5881 ccagcctcaa cggcgggaca acgcccgtgt cagcgtttac atcgacggca tgtttgcctt
     5941 tggcctgcat attgacctcc agctcgagca ctacctgaaa aaaggcgacg tgctggatga
     6001 ggaaaagatc gcgctcctgc tcaagcagga cgctgtcaag aaagccatct ccacgtccct
     6061 caacctcatc gcctatcgcc ctcgttcggc gggcgagttg gcaagcaagc ttcgcgagag
     6121 gggctacgac gcggaaacca ttgatgaagc gattggtcgg atgagggagc tgaactacct
     6181 cgacgatcgg gagtttgcgg atcgatgggt ggaatcccgc cagatgcatc ggcctcgatc
     6241 aacccggatg ctcaggcagg aactgcagca aaagggcgtt gaccgtcaga tcatcgaatc
     6301 gaccatcgag ggggcgggta tcgacgagtt cggggacgcc ctcgcgcttg gacagaagaa
     6361 agtggaatca ttccgggcgc ttgagccgca ggtacgggac cggagactga gcggcttcct
     6421 tgcgcgccgc ggctacagct acgacgtgat ccggcgagtg ctggaggcgc tggagaccgg
     6481 ggagacagta gacggccaat agcgcgggat cacggcattc gctctcacgc aacagattcc
     6541 tgattcctga ccttgggaac tggtgccagt gccatggtat gatagaaaga ggctgcggaa
     6601 agacgcgagt cgatcgacgc caaacctttc tttcttcccc aaatgactct atacgtatgc
     6661 aggctcgttc gcgccattgg tcctgaccac cggtgcccac gggaacagcg tgtttgtatt
     6721 tactgatcag gtggtatcgc tgcgcggcac tcttcgccat gcgggtcggc caccagggag
     6781 cacccccggt ctatggctga gattcgttgg cttgggcaca actgtttccg cattcgagcg
     6841 cgggaagcga ccgtgctgat ggatcctgtt ggcaagaaga ccggattcac catggcaagg
     6901 caaacggctg atatcgtcac cctctcgcat cagcatgccg gccacacaaa ccttgaagca
     6961 gtgaagcccg agtaccaggt gattgacggc ccaggtgagt acgagcttca tggcgttttc
     7021 atcaccggca tccggtcgta tcacgacagg aacaagggca ccgagggtgg ctacaacacc
     7081 atctatcgcg tggaattcga tggcctcaag ttcggtcatc tcggcgatct tggtcatccg
     7141 ctgaacgacg accaaatcga atcgctttcg agcgtggata tcctctttgt accggcagga
     7201 ggcggtccac tgctcccgcc atctgagatg gcggaggtcg ttggcggcat ctcgccacgc
     7261 atgatcatcc ccatgcaata tcgcgccggc aagggcgacc aaagccggga tccagtggaa
     7321 cccttctgca agcatctcgg ggctgaagtg ccgcagccgc ttgagaagct cgtcgttaag
     7381 gcatctgacc tcgcagatca gatgcagctc gttctgctta cgcccgacgg ctaagggctg
     7441 ggcacatcgc ttgcatcgtt atttgtcctc gtgctgaatc ctgaaacgtc ccaggaggac
     7501 ctggatgcct aaatacgtat tcgtgaccgg tggtgtggtg tcgtcagtcg gcaagggcat
     7561 caccacggca tcgctcggac gcctcatcaa gaaccgcggc gcgagcgtct cgatcatgaa
     7621 gctggacccc tatctcaacg ttgatccggg cacgatgtcg ccctaccaac atggggaggt
     7681 cttcgttacg gatgatggtg cggagacgga tctcgatctc ggtcattacg agcgcttcat
     7741 cgacgagaac ctttcccgcg cctccaatgt gacgtccggc caactctact catccgtcat
     7801 tgcgaaggag cgtcggggcg attatctcgg cggcacgatc caggtgatcc cccacatcac
     7861 caatgagatc aaatcccgta tccggctcgc ctcgaggcaa cacgattcag atgtcgtgat
     7921 cgtcgaggtt ggcggcactg tgggtgatat cgagggccaa tcctatctcg aggccattcg
     7981 gcagatgcgc cgggagctcg ggcgtggcaa cagcttgagc atccatgtca cgctcctccc
     8041 ggaagttggc gccaccggcg aactcaagac caaaccgacc cagatgagcg ttcgcgagct
     8101 tcgcggcatg gggatccagc cggatgtcat catctgccga gccgaccgag agatgccagg
     8161 cgacgtccgg gacaagatcg cgctcttctg cgatgtcgag caggacgccg ttatcggcat
     8221 gccgactgca tcatcgatct atgaagtacc gctgattctc gaggatgccg gcctgggccg
     8281 ttacgtcgcc aaggcgtttg gcctcccgca ggagccgaac ctgcgcgagt ggacaaacat
     8341 ggttgagcat attcatgagc cccggaaatc gctccgtatt gcgcttatcg gcaagtacgt
     8401 cgaattgcat gatgcctacc tgagcgtcta cgagtccctg atgcacgctg gacttgccaa
     8461 tgacgtcaac atcgaggttg actggatcaa ctccgagaca gtgacccagg aagaactcga
     8521 gcgagagctc cggagggtgt cgggtgtcct cgttccgggt gggttcggtc ctcgtggcat
     8581 tgaggggaag gtgtcgggtg tcacgttcgc gcgcgagcac aacatcccct accttggcct
     8641 ctgctatggt cttcatatgg ccgtgatcga gattgcccgc aacctgtgtg gccttgaggg
     8701 tgccaactcc acggagatcg atgcgaacac gccccatccg gtcatcgacc tgatgcctga
     8761 ccagaagaac gtggacatgg gcggcaccat gcgtcttggt ttatgggagt gccgcctgca
     8821 gccggggacg aaggccgccg atgcctacgg agcgccttcg gtccacgagc gacatcgcca
     8881 tcgattcgag gtcaacaatg cgtaccggga tcaactggag ggagccgggt ttaccgtctc
     8941 cggcgcatcc ccgaacggcc agctcgccga gatcatggag ctcagggacc atccgttctt
     9001 cgtcggtgtc cagttccatc ctgagttccg cagccgccct aaccggccac atcctctttt
     9061 cgtggacttc atctccgccg ccaaggaggc gctgccggaa ggcgcccagc gtctcctgcc
     9121 gctggaaaag gaactcctgc cggatagcaa acttatgata gaagtgcgcg ccgggcagga
     9181 gatggcgact cactaacctc aagcatagat ggatgcagaa cacccgcatg gaaatccatg
     9241 cgggtgttct gcatctggct caccaagcct cggttctgct ctcgggacca gccccctgcc
     9301 cgctactcct cagccgtatc gtggcgggac gcgtccctgg gaagctccag tgtgaagatt
     9361 gccccaccac catcggcgtc atcgacggtc agcgagccgc catgggcctt ggccagtcgc
     9421 gcactaatgt agagaccgat gccgaggcca tggctgccat tggtagcggt tcctccatca
     9481 tcctggccat cgtcgaaccg gtagtactgc ttgaagagcc gcttctggat cttggcaggg
     9541 actccgggac catagtctcg cactgtgatc tttaccctgt ccgttcccgt ctcacagaga
     9601 ttcacctcga ctggtccgtc aggagcgtac cgggcggcat tctgcatcag gttgccaagc
     9661 acctgttgca cacgggcggc gtcccagaca accggtaaca cgtctgggcc ttcaagaacg
     9721 aacggccggt ccgggttcag tccctttgct tcagtgaccg ccaaacgcgc cagcatgctg
     9781 gcgtccgttt ccgccacggc aatatccagg cttcccagat cgacacgcga cacatccagc
     9841 atccggctaa cgaggtccgc aacacggtcg acctgggtga taaccgaatc cagtcgctcc
     9901 agctcatcat ctgaatgctc gtccgcgcgc ttgcgaatac gccgctgcat caactgcagg
     9961 ttgccgcgca aggacgtcaa tgggttgcga agctcatggg ccaccacgga aataaactcg
    10021 tctttcgcac gatcaagcgg cttgaagtcg gaccgatcct gtaggaggcc caccgatccg
    10081 atgatcacac cacgcccgtc aaaaatgggc gcatgattcg ccacgacctc cacctgcgtc
    10141 ccgtcatcct ggaccacccg gagtggttgg ccgatgacct ccttgtgggt ctgcaatgga
    10201 cgaagtaacg ggtgatcctc gccgacgaga ggaatgccgt cagcatccac aactggcaag
    10261 ctctcgggat ggatttgccc gtcctccagg gatcgtcccc acagacggtc tgctgcctgg
    10321 ttggcaacgg tcaccacacc ggtgggctca tcgagaatga ggaccgcttg cggaagctga
    10381 tcgatgatcg ccatcagccg accatgtgcg tactccagct ggccaaccaa catcaccttg
    10441 tcgatcgcct gcgcgcactg acgtccccag attgcgccga aggcgagatc cgagtcatcc
    10501 agcatgggct gcttcctgaa gcccacgaac gctacaccaa tgcgctgtcg gtgcatctgc
    10561 atcgggatga tcatcgcaac agcctcatcg ccgagctctg acgccattgg ctccggcacg
    10621 cgcagttggt tcaggggaac aacaagcggc cgataacgtg tgaaggcgcc ggctaccacc
    10681 ggggatgcca ggatatccat gggccacgat gggaagagct ccggacgaac acctcgacag
    10741 gcggacaggt agaagtgatc gtcctcgcca tgcagcatga tggcgccgca atcagcatcc
    10801 aactcctgcg tcagccgtct caaggcggtg tcaacgactg tctgtcgatc ccggcccgtg
    10861 ttgatctcca ccacgaggtc ggcaatggcc tgggaacggt gggccgcgcg ctccagctgc
    10921 tgcagttgct gcctgtcccg aatctcagca cgatgctccg gggtgacgtc gcgcgagaca
    10981 gagatggcac caacgatctt gtcatgggcg atgatcgggc gtgtactcgt cctcagcagg
    11041 agtgtctccc cattgttccg ggtgatcacc tggtccaaag tctcaggccg ctcgccgtgg
    11101 agcacttgca tcactggaga atcgttaatg tcggtttcag ttgcgtccag accgtgccgg
    11161 aacttggtcc tgaggtactc ggtggcatgc tggcccagcc gggattcctc ctcgctgaga
    11221 tcgaacagcg ctcgctcaga tgaattggag agagtcagga acccctgctc atcgaatgcc
    11281 gagactgcgt tggcgagctc atcgagaatc gcttccatct gcagccggcg tcggcgctcg
    11341 ttctgccgca gcctggtgtt gtccagggcg atagcgacct ggcttgccac cccgtcagca
    11401 agggccaatt gctcctcggt gaagtcaata ccatccccgt aacggtcgcc gaacatgact
    11461 cccacgattc ggttcttgcg gaggagtggg actaccaaaa gcgggccaga cggaaaggtg
    11521 taaccggctt caagcgggac gtcttgcagc ggatcggctg tgatccttgc acttggctcc
    11581 tccgtgagcg cagagaatat cggaaacgcg tccagtgcca ccgtatcggg gaggttcgaa
    11641 agaggttcgc ggacgactgc caaacgcgtc cctcggttga cctcggcgtc gaccagccag
    11701 agagacatgc gttccagata caatgcttca gccacctgct cggtcgcgat ctgggccacc
    11761 tcttcgatgg ttccgtcact tgctactgaa tggatgcctt catagagtgc tgcgaggcgt
    11821 ctcgccagcg tgagatgatc gggagtcagg aggctcccat ccgatccggg ctcatggccc
    11881 tcggcctgct cactacgata tttgcgcggt gcgcggtcct tcattgggcg ccaggcatgg
    11941 agatcgcgtg ccgagatcag cgccatacgc cccaagcgtt ccacaggcaa acgtcccttc
    12001 cgtaccgcac gcgagacggt atggtaggaa acacccttga gccgggcggc ctcggccatc
    12061 gtaaggcgat ccctatcaag gttcacattc ccatcagaat ggcgttgagt tccgttgata
    12121 ccgttgttca tgagcaacca gcccttgccc ggtgagacct gttggccatc accaccccga
    12181 ggatcacaaa caggtcttgc ccggatacac caggatgtgt cgagcgacaa caagtgggca
    12241 gatgcacgct cctccgtgcc caccttgcgc agacccgtaa atcctcagca catcgcatgt
    12301 gtccacagtt ttgcacacct tcggcgatgt tgccagagca ccggatggct ctctcaaact
    12361 gccccgggtt tacgttagtc cagcatctgg t
//
LOCUS       DASYWM010000283         7599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_50883, whole genome shotgun sequence.
ACCESSION   DASYWM010000283 DASYWM010000000
VERSION     DASYWM010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50883"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1211)
                     /gene="argC"
                     /locus_tag="VGR29_11540"
     CDS             complement(162..1211)
                     /gene="argC"
                     /locus_tag="VGR29_11540"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEV2074260.1"
                     /translation="MALDVSIIGGSGYAGGELVRLLLSHPEVNIKQVTSERQAGKFIR
                     TTHPNLRKRCDLKFVSMSKLEPCDVLFLALPHGVSMQKLPEVRDLAPIIIDLSSDFRL
                     NDPEDYPTWYSHEHNDLEALGTFVYGCPELHREEIRSANWIASPGCMSITTILGLYPL
                     FQAGVVDLDMPVVTEAKTGSSGSGGSPSPASHHSERSGVVRSFKATGHRHTAEIIQEL
                     TFDGAKPSMAFSATAIEAVRGILTTSHVFLKDDLQDKDLWKIFRGAYKEEPFMRLVKE
                     NAGVHRYPEPKLLSGSNWCDVGFEKDPHSKRVVVMAATDNLMKGASGQAVQSFNIRMG
                     FPEETAIDFPGLHPI"
     gene            complement(1233..2153)
                     /gene="lysX"
                     /locus_tag="VGR29_11545"
     CDS             complement(1233..2153)
                     /gene="lysX"
                     /locus_tag="VGR29_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEV2074261.1"
                     /translation="MVQEQRPLHVGILMSRVRVEEKLLLAELERREVEIVRFDDREYT
                     LDLAHPHPELAACDVVLERCINHLRALYSLRALNDWGIPTVNTYDVANICGDKLLTTA
                     ALVRDGVPVPRTRIAFTPESALEAIEELGYPVVLKPAVGSWGRLLARVNDRDSAEALL
                     EHKVTLGSFHHGAFYIQEYVEKQGRDIRSFVVGDETICAIYRESPHWITNTARGARAS
                     NCPITPELDAMSRAAAQAVGGGVVAVDLFETPTGLQVNEVNYTMEFRNSIHTTGVDIP
                     AKVVDYVIAVGEEYRAKNADRAEAALVAYK"
     gene            complement(2557..3474)
                     /gene="lysX"
                     /locus_tag="VGR29_11550"
     CDS             complement(2557..3474)
                     /gene="lysX"
                     /locus_tag="VGR29_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEV2074262.1"
                     /translation="MMGIERGAGLRLGVLVSYLRQEEKLILAAARARGMIVTPIFDRE
                     LSLNLSAPSAAESGVDIDVLLDRSVVHSRAGYTLWAMNRWGVPTLNSPAAVTICDDKA
                     RASMVLEAAGIPSPRTYVAYSVESALRACEELGYPAVLKPVAGSWGRLIAKVNGPDQA
                     RAIIRQKSEHGSFHHEIYYIQEFIQKPGRDIRAYVVGDEIVAASYRTSEHWITNAARG
                     AVSVPCPVTPEIEELALRACKVVGARLAGVDLIETDEGLKVIEINTGGEFKGLMTTTN
                     RDIAGEIVEEAAKVAREGVDDPLTAAAAG"
     gene            complement(3543..4628)
                     /locus_tag="VGR29_11555"
     CDS             complement(3543..4628)
                     /locus_tag="VGR29_11555"
                     /inference="COORDINATES: protein motif:HMM:NF003367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-lysine hydrolase"
                     /protein_id="HEV2074263.1"
                     /translation="MPSLSPHSIEAPWTAQERADIELLRSLIAIPSLSGQEENAVEYL
                     RGAMASRGFDVLDDASGSAVGRIGSGEKHIILLGHIDTVPGHIPVQIRDGVLWGRGAV
                     DAKGPLATFVTAASAAADRLNATVTVVGAVGEEVIGSPGANEVAKWPSPDFCIIGEPS
                     GWDAVCLGYRGSFSLIYTLKRSTRHTAGPGQSVAQEAVDFWNALQAEVAEWNAEAKGN
                     FWSVGPSLRAFNTTGNGLEDVATLSIGLRLPPELDVARLKRRIEEIAGDAELQIDGLQ
                     EGYRTTRRSKLIPPFMRAIRAHGGAPRFTLKLGTSDMNVVGPAWGCPIVAYGPGDSSL
                     DHTPEERIDLAEYLRAVRILGDVLASF"
     gene            complement(4606..5490)
                     /locus_tag="VGR29_11560"
     CDS             complement(4606..5490)
                     /locus_tag="VGR29_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074264.1"
                     /translation="MTTAIGVLCARVRVEEKQIITALADAGAVAMPVPPASLPLPSCP
                     APRDLAALGKQSAQGNGANPVPGVIVDRSANRSVAGALLQLLKRAGVEVIDAGLASRG
                     SRLDVAASLMSAGLPRPASLVAFSELSGLQAAASVGFPSTLLPIKPGSTTTVLLDQDT
                     ADAVIEHRVVLGSEDEALILVQAGAPTSADLVRIHVVDGKAIAFEGEEPTTAAIDLAQ
                     SAAQVLDALFASIDIACINGECLVWDVIPVADFRQSTLLGDVTVGGALAALATERASR
                     VAMKAGWEGITHAIALTA"
     gene            complement(5577..5744)
                     /gene="lysW"
                     /locus_tag="VGR29_11565"
     CDS             complement(5577..5744)
                     /gene="lysW"
                     /locus_tag="VGR29_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HEV2074265.1"
                     /translation="MAMATCPECSGEINVENVEVGEIIPCGECGADLEVTGTDPVELS
                     LAPEEGEDWGE"
     gene            complement(5836..6882)
                     /gene="argF"
                     /locus_tag="VGR29_11570"
     CDS             complement(5836..6882)
                     /gene="argF"
                     /locus_tag="VGR29_11570"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV2074266.1"
                     /translation="MTMVSASEARTIRYQMAGEEHELPLLPSLHRRSLLKDTDLSSEE
                     ILAIIQTASMLKMLRDSHISHAWLGGQTLGMIFQHPSTRTRTAFQAGMEQLGGQAIFL
                     GVQDLQLKRGETIEDTARVFSRYVDAVAARVADPVDLVQFAAGATVPVFNGLTSIDHP
                     IEALSDVMTLYEHFGTLRGLTFAYLGDGNNVCHSLMLAMVASGVNVRIAGPKAYGPDP
                     EIVATAQQIAVTTGASLELVDDPRVAVDGADAVYTDVHESMGEQENPAKLMALAPYRV
                     TEELMAQATPEAVFMHCLPLHRGQEVDSAVADGPQSIIFDQAESRLHVHKAVLLQVLY
                     DERRIRNDEVVPQA"
     gene            complement(6893..>7599)
                     /gene="argF"
                     /locus_tag="VGR29_11575"
     CDS             complement(6893..>7599)
                     /gene="argF"
                     /locus_tag="VGR29_11575"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV2074267.1"
                     /translation="FLGVNDLQMKRGESIGDTAQILSRYVDALVARVADHDDLIQLAT
                     SASIPVMNGLSDQCHPLQAFADLLTLQENFGELKGVKLAYLGDGNNMAVSLMLSGAAM
                     GISVSVAAPKSLQPDPVVVQHAQWLGSSTGATVEVTDDPLVAAKDANAIYTDVHVSMG
                     QEDAAGRAVALAPFKVTPDVMGAAAKGAVFMHCLPMHRDEEVSVEVADGPQSVIFDQA
                     ENRLHMHKALLVHTMC"
BASE COUNT         1484 a         2534 c         2146 g         1435 t
ORIGIN      
        1 ctcgaagaac cgcccctcgt catcctgaac ggatgaaacg accccggcca gtcgaatccc
       61 atcgaaggct cttgacgcta cccagccaag tccttcgcca agagcaaaga ttcctcggct
      121 tcgctcggaa tgacggagac ggttcggcgc cggttgtctc gttaaatcgg gtgcaggccg
      181 gggaagtcga tcgcggtctc ttccgggaag cccatccgga tattgaacga ctgcaccgcc
      241 tggcccgagg cgcctttcat caggttgtcc gtcgcggcca tgaccaccac acgtttggag
      301 tgcggatcct tctcgaaccc gacatcgcac cagttcgagc cactcagcag cttcggttcc
      361 ggatagcgat gcacacccgc gttctccttc accagccgca tgaagggttc ttccttgtac
      421 gctccgcgga aaatcttcca gaggtccttg tcctgcaggt catccttcag gaagacgtgc
      481 gacgtcgtga ggattccgcg cacggcctcg attgcggtgg ctgagaaggc catggacggc
      541 ttcgccccat cgaaggtcaa ctcctggatg atctcggccg tatgtcgatg accagtcgcc
      601 ttgaacgagc ggacgacacc actgcgctcg gaatggtgag atgcaggact cggagagccg
      661 ccagagcccg aagatccggt ctttgcttcg gtcaccaccg gcatatcgag atcgacaacg
      721 cctgcctgga agagcggata gaggccaagg atcgtggtga tcgacatgca acccgggctg
      781 gcgatccagt tggccgagcg aatttcctcc cgatgcaact ccgggcaacc atagacgaac
      841 gtcccgagcg cctcgaggtc gttgtgctcg tggctgtacc acgtcgggta atcctcggga
      901 tcgttgagcc ggaagtccga gctgaggtcg ataatgatcg gcgccaagtc ccgcacctcg
      961 ggcaatttct gcatggagac accatgtggc aacgcgagga agaggacgtc gcacggctcc
     1021 aacttgctca tcgaaacgaa tttcagatca caccgcttgc gcaggttcgg gtgcgtcgtg
     1081 cggatgaact tgccggcctg tcgctcggat gtcacctgct tgatattcac ctccggatgc
     1141 gacagcagaa gccggaccaa ttcacctccg gcatagccgg atccaccgat aattgaaacg
     1201 tcgagtgcca cgagaaaatc ctcccgaatt ccttacttgt acgcgaccag ggcagcttct
     1261 gcccgatcgg cattcttcgc gcgatattct tctcccaccg cgatcacgta gtcgacaacc
     1321 ttcgcgggga tatccacccc ggtcgtgtga atgctgttcc ggaactccat ggtgtagttg
     1381 acttcattga cctgcagacc ggtaggcgtt tcaaacaggt ccacggccac cacgccgccg
     1441 ccaacggcct gggccgctgc ccgtgacatg gcatccagct cgggtgtgat cgggcaattg
     1501 ctggccctgg ccccacgcgc cgtattggtg atccagtgtg gcgattcgcg atagatggcg
     1561 catatcgtct cgtcacccac gacaaaggag cgaatatcgc gtccctgctt ttccacgtac
     1621 tcctggatat agaaggcgcc gtggtgaaac gacccaaggg tgaccttgtg ctccaggagc
     1681 gcctcggccg aatcacggtc attgacgcga gccagcagcc gtccccagga gccgacggcc
     1741 ggcttgagca ccaccgggta ccccagctcc tcgatcgcct ccagtgctga ttccggcgtg
     1801 aaggcaatgc gtgtccgggg caccggcacc ccgtcccgaa ccaacgccgc ggtcgtcagc
     1861 agcttgtcgc cgcagatgtt cgccacatcg tacgtattga ccgtcggaat accccagtcg
     1921 ttgagcgccc ggaggctgta gagagcccgc aggtgattga tgcatcgttc caacacaaca
     1981 tcacacgcgg cgagttcggg atgggggtgc gccagatcca gcgtgtactc ccgatcgtcg
     2041 aagcggacga tctcgacttc tcgccgctcg agctccgcca gcagtaattt ctcttccacc
     2101 ctaacccggg acatgaggat gccaacgtgc agaggtcttt gttcctgtac catccggaac
     2161 gtcctttcct ggcgactatt ggcgccagtg tgtgaataga cggccctgtg tcctaccagc
     2221 attctgatga cttgccgggt gctggaaggt tcttccgccg gacgacaacc aaaatgccgg
     2281 tcaagaggac gggggccgct ccgtggtacc acctctgttc gcgatgccct cacgggcact
     2341 cgcctttgcg ggtgcacatg cttatgtgca cccatacccg ctaacggagg tcaccgaccg
     2401 gttctactga tcggagggct ctccgatgtt cttccggttg ctcagggacc ttcactctat
     2461 cgtccgtacc gcggcgcttt caccatctcg ccgctcgctg gggccggttt cgataccgcg
     2521 ttcccttcat cgccatcttt gattgtcgct gaccggttaa cccgccgcgg cagccgtcag
     2581 tggatcgtca accccctcgc gcgcgacctt tgcggcctcc tcgacgatct cacccgcgat
     2641 atcgcggttc gttgttgtca tcaacccctt gaactcaccg ccggtgttga tctcgattac
     2701 cttcagcccc tcatcggtct cgatcagatc aacgcccgcg agccgggcac cgacgacctt
     2761 gcaggcccgc aacgcgagct cctcgatctc cggcgttacc gggcagggca cggagaccgc
     2821 tccccgtgcc gcgttggtga tccaatgctc cgatgttcgg tatgacgcgg caacgatctc
     2881 gtcaccaacg acataggcgc gaatgtcacg gccgggtttc tggatgaact cctggatgta
     2941 gtagatctca tgatgaaagg agccgtgctc cgatttctgc cggatgattg cccgcgcctg
     3001 atcgggacca ttgaccttcg cgatcagccg gccccaggag cccgcaaccg gtttcaacac
     3061 ggccggatac ccgagctcct cgcaagccct cagggcggac tccaccgaat aggcgacata
     3121 cgtcctcggc gacgggatgc cagcagcctc cagcaccatg ctggcgcggg ccttgtcatc
     3181 acagatcgtc acggccgcgg ggctattcag cgtcgggacg ccccaccggt tcatcgccca
     3241 cagggtgtag ccggcacggg agtggaccac gctgcgatcg agcaggacat cgatgtcgac
     3301 accgctctcc gctgccgagg gtgcggagag gttcagcgag agctcgcgat caaagatggg
     3361 cgtcacgatc atgccgcgtg cgcgggccgc cgcgaggatc agcttttctt cctgccggag
     3421 ataggagacg agcactccca gccgaagccc cgcccctcgt tcaattccca tcattcacct
     3481 gttcgcggcc tttggccgca tggatggaca cgagatccac ccctttgggt atcgcacgtc
     3541 cattaaaacg acgccagcac gtcgcccaaa atccggactg cccggagata ttccgccagg
     3601 tcgatccgct cctcgggcgt atggtcaagc gacgaatctc ccggaccata ggctacgatc
     3661 gggcagcccc atgccggtcc gaccacattc atgtcggatg tccccagttt gagtgtgaaa
     3721 cgtggggcac caccatgcgc gcggatcgcc cgcatgaaag gcgggatcag cttcgagcgc
     3781 cgggtcgtcc tgtacccctc ctgcaaacca tcgatctgaa gctccgcatc gcccgcgatc
     3841 tcctcgatac ggcgcttgag acgggctaca tccaactcgg ggggaagccg cagcccgatc
     3901 gagagggtcg ctacatcctc aagcccatta cccgttgtgt tgaaggctcg caacgatggc
     3961 ccaacggacc agaagttccc cttcgcctcc gcgttccact cggccacctc tgcctggagc
     4021 gcattccaga aatcaacagc ctcctgcgcc accgactggc caggaccggc cgtgtgacga
     4081 gtgcttcgct tcaatgtgta gatcagtgaa aaggagccac ggtagccgag gcagactgca
     4141 tcccagccgc tcggctcgcc gatgatacag aaatccggcg agggccactt cgctacctcg
     4201 ttcgcaccgg gcgagccaat gacttcctcg ccgaccgccc caacgacggt gactgtggca
     4261 ttgagccgat ccgccgcggc actggccgca gtaacgaagg ttgcgagcgg gcccttggca
     4321 tcgactgcgc cacgtcccca gagaacaccg tccctgatct ggacgggaat atgaccgggg
     4381 accgtatcga tgtgacccaa caggatgatg tgcttctcgc cagaaccgat cctgcccacg
     4441 gcactcccgg aagcatcatc gagaacatcg aaaccacgcg atgccattgc gccgcgcagg
     4501 tattcgacag cgttctcctc ctgcccggag aggctgggaa ttgcgatcag cgaccgaagc
     4561 agctcaatat ccgcccgctc ctgcgccgtc catggtgctt cgatgctatg cggtgagagc
     4621 gatggcatgc gttatcccct cccagcccgc cttcatcgca acccgggacg cacgttccgt
     4681 cgccaatgcc gccaatgctc caccgacggt cacatcgccg agcaaggtgc tctgccggaa
     4741 atccgcgacc gggatgacgt cccagaccag gcactcgcca ttgatgcaag cgatatcgat
     4801 gcttgcgaac aaggcatcga gcacctgtgc cgcgctctgt gcgagatcaa tagctgcggt
     4861 agttggctcc tcgccctcga aggcaattgc cttcccatcc acaacatgga tacgcaccag
     4921 atcggcggag gtcggtgcac cagcctgaac caggataagt gcctcgtcct cgctgccaag
     4981 taccacgcgg tgctcgatca ccgcgtcagc ggtgtcctga tccagcaaca ccgtcgtggt
     5041 gctgcctggc ttgatcggca gcagggtcga gggaaagcca accgatgccg ccgcctgcag
     5101 cccggaaagc tccgagaatg ccaccaggga tgccggacgc ggcagacccg cactcatcag
     5161 ggacgctgcg acatcaaggc gtgaacccct tgaagccaaa ccggcatcga tgacttcgac
     5221 tccggcgcgc ttcaacagct gcagcagcgc accagcgacc gagcggttgg cgctacgatc
     5281 gacaatcacg ccgggcacgg ggttcgctcc gtttccctgt gccgactgct tgcccagcgc
     5341 ggcgagatcc cgtggcgccg ggcaagaggg caacggcaag ctcgcgggag gcacaggcat
     5401 cgcgaccgct ccggcatcgg cgagggccgt gatgatctgc ttttcttcca cgcgaacgcg
     5461 tgcacacagc acgccaattg ccgttgtcat tgaatcgttc ccccaacgtg cagggtcgac
     5521 cacaaggcct ccctgggcag cctcattggc ttgcccctgc aacacgactg aaagggctac
     5581 tcgccccagt cctcaccctc ttccggggcg agcgacagct ccaccggatc ggttccggtg
     5641 acttcgagat cagcaccgca ctcaccgcac gggatgatct cgccaacctc gacattctcc
     5701 acattgatct cgccgctgca ctccggacat gttgccattg ccatcgcttc ctatccctct
     5761 agcctcatga ggcttcacta cttcggtgtg tccccacgga cacgaacaca taccaatacg
     5821 cttgatctga cgaactcagg cctggggaac aacctcgtcg ttcctgatcc gacgctcgtc
     5881 atacagcacc tgcagcagca ccgccttatg gacatgcagc cggctttccg cctggtcgaa
     5941 gatgatcgac tgcggaccat ccgcgactgc ggaatcgact tcctgcccac gatgcaacgg
     6001 caggcagtgc atgaagaccg cctccggcgt cgcctgcgcc atcaactcct ctgtaacccg
     6061 gtagggtgcc aacgccatca gtttcgccgg gttctcctgc tcgcccatgc tctcatggac
     6121 atcggtatag actgcgtcgg caccgtccac agccactcgc ggatcgtcga ccaactccag
     6181 acttgcccct gttgttacgg caatctgctg cgctgttgca acgatctccg gatcaggccc
     6241 gtaggccttc ggcccggcga ttcgtacatt aacccccgat gccaccatcg caagcatcag
     6301 ggaatgacag acattattac cgtcgcccag atatgcgaac gtcaagcccc gtagcgtgcc
     6361 gaaatgctcg tagagggtca tcacatcgct cagcgcttca atcggatgat cgatggatgt
     6421 gagcccattg aagaccggga ccgttgcccc ggcggcgaac tgcacgaggt cgacggggtc
     6481 ggccacccgg gccgcaaccg cgtcgacata ccggctaaag actcgcgcgg tgtcctcgat
     6541 cgtctccccg cgcttcaact gaagatcctg caccccgaga aagatcgcct gcccgccaag
     6601 ctgctccatc ccggcctgaa acgcagtccg cgttcgcgtg ctcggatgct ggaagatcat
     6661 gccaagcgtc tggccaccca accatgcatg ggagatgtgg ctgtcgcgga gcatcttcag
     6721 catggacgca gtctggatga tggccaggat ctcctccgac gaaagatcgg tatccttcag
     6781 caggcttcga cgatgcaacg acgggagcaa cgggagctca tgctcctcgc cggccatctg
     6841 atatcggatt gtgcgcgctt cgctggctga taccatcgtc atggccatgt ctctagcaca
     6901 tcgtgtgcac aagcaacgcc ttgtgcatgt gcagccggtt ctcggcctga tcgaagatca
     6961 ccgactgcgg accatccgca acctcaaccg agacctcctc gtcccggtgc atcggcaggc
     7021 agtgcatgaa gaccgccccc ttggccgccg cgcccatgac atcgggcgtc accttgaacg
     7081 gtgccagggc aaccgcccgg ccagcggcgt cctcctgccc catgctgaca tggacatccg
     7141 tgtagatcgc attcgcatcc tttgctgcga ccagggggtc gtcggtgacc tcaaccgtcg
     7201 cgcctgtcga actgccaagc cactgggcat gctggacgac aacgggatct ggctgcaatg
     7261 acttcggagc cgcaaccgag acactgatgc ccatcgctgc cccggaaagc atgagcgata
     7321 cggccatgtt gttgccatcc cccagatagg cgagcttcac gcccttaagc tccccgaagt
     7381 tctcctgcag ggtcaggaga tcagcaaagg cctgaagcgg atggcactga tcggagaggc
     7441 cgttcatgac cggaatcgag gctgatgtgg cgagctggat caagtcgtcg tgatcggcga
     7501 cccgggcgac aagcgcatcg acataccggc taagaatctg ggccgtatcg ccaatgctct
     7561 cgccgcgctt catctgcagg tcgttcacgc cgagaaaga
//
LOCUS       DASYWM010000284         9329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_50968, whole genome shotgun sequence.
ACCESSION   DASYWM010000284 DASYWM010000000
VERSION     DASYWM010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50968"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2035
                     /locus_tag="VGR29_11580"
     CDS             <1..2035
                     /locus_tag="VGR29_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEV2074268.1"
                     /translation="HRRMFFRALRWEYTIMSLPTWLLLLSGLLAVAVLLGVLARRAHI
                     PLTVVLALVGFLAGWLGDIESPISAERFEEVLVFVFLPVLVFEAAFSLSTRAFFRNLV
                     SILVLAIPALVISAICVGLSLYGFLDTPLIPALLFGALISATDPVAVVAIFRELGVPR
                     RLLTLVEGESLLNDGVAIVLFTILLSGALGGDLAPAEGMWNFLVVFFGGALIGAAVGL
                     LAALALPWLDRLSAAALSMAVAYGGFVLADDVLGFSGVTATVAAGLVLGGSAPIRATA
                     PVRDMWESLWDTLGYVANALLFLLMGLAIDPALLTENLGAIGLAITAVFVARAVAVVP
                     LMTLLERVAGIPPVGIRNEAVLIWGGLRGGVALALALALPEDLAQRDQFVAMTGGVVL
                     ATLLLNATTIGALVRRLRLDEPSPADRFLAGGARLSAIAAARTRLVDLRLEDPVVVSE
                     LDHAEELAHAELARLSLDSVDEIHVLIRRGLVVERETYQRLSDAGLLPPTAARLLLHE
                     VDDQIEALGVGRVTLASVYQRERPAFDRLMARLASSLPSPLGDDPGSVAFAEASARRL
                     AARRTSEAFELFTRLPNIGADTIKEARATFAWWEAEAEARLTELDTDVTQDMRQVQHQ
                     QAEKLIHMASTDALEDLAEIGLLPARVARRAAETVAAEIGNGRSQPGGAVHEQ"
     gene            2441..3214
                     /locus_tag="VGR29_11585"
     CDS             2441..3214
                     /locus_tag="VGR29_11585"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEV2074269.1"
                     /translation="MFAVISLLAILSLSLLVVRIGAIALMMTGMSRDAAAFQALSAFS
                     GTGFTTSESELAVTTPERRKIIALLIRAGSAGAITVVTSLILSFVGSEDRAPDRLLII
                     VGGLVGLVTLSRSGWFNRMLTPIIQRALSHSTPLVLRDYAALLHLREDYRVAEFEVRP
                     GGWLEGRQLRDLDFPAEGLLILGIVQPSGEYVGAPPTDFVFSSGDTVVVYGREPNLEE
                     VAQRRSSDSRAHEEGKADHAGEVDAKTHTDIDQDAHRSR"
     gene            complement(3662..5122)
                     /locus_tag="VGR29_11590"
     CDS             complement(3662..5122)
                     /locus_tag="VGR29_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2074270.1"
                     /translation="MNVSNDLLQASLEDLYEQGPCGYVFTGLDGMILRVNDTLLNWIG
                     AVREDLIAVRRFQDILTVPGKLFYENQIFPLLRLQGSVQGAAFDLARRGREPLPVLVN
                     SQQRTPDDGRPVLIAHAIFDATDRRAYEQELIRSRRSSEQLAAMVKMSSDAIVSMSDE
                     GVVTTWNAAAERLFGYPAHEIIGKDLRQILAPVKEHAPWQGIWAELRSAHAVQLEMVG
                     SCMDGRTVEVSAGFTPHPDLLGNLNAVSVIMRDIAERRAVERLQHEFLAVTTHELRTP
                     LTGIRANAQLMQRRAAYNERAVAAIIAQADTLQRLTDDLLLASRIQADHLTLVLGQTD
                     LAAVARAAVESLGADESVIRVEAPEGALMVSADQQRLGQVVTNLLTNAIKYSPEGSKI
                     TVRIAPHMQEARISVADHGVGIPPDALPHVFERFYRVAGGTGRVPGLGLGLYISRRIV
                     EAHGGRIDVESAPGYGSTFTVTLPMQPNLTERAVTV"
     gene            complement(5133..5948)
                     /locus_tag="VGR29_11595"
     CDS             complement(5133..5948)
                     /locus_tag="VGR29_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2074271.1"
                     /translation="MSALTRNNVTLVGNGDTPIMFAHGYGCDQNVWRLIIPAFVDTHR
                     VVLFDHVGAGRSDLLAYDRAKYSSLRGYAQDILEICDQLDLKHGIFVGHSVSAMIGLL
                     AAIQEPERFDHLVLIGPSPCYINDGDYFGGFSREDIDDLLELQDSNYLGWASSLAPVM
                     MGNPDRPELAENLRNSFCRTDPDIGKQFAAVTFLSDNRADLPKARVRSLILQCHADTV
                     APLAVGEYVHRHLSDSDMVVLDATGHCPHLSAPEETIEAIKVFLGSQKSCGIR"
     gene            complement(6960..7706)
                     /locus_tag="VGR29_11600"
     CDS             complement(6960..7706)
                     /locus_tag="VGR29_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV2074272.1"
                     /translation="MEFHVALQAYTVRNELAGDYLGTLTKIAEIGYEGVELGPPPADI
                     SLDQLKHHLEQIGLRVVGSHGGLQQLTDDLGTQVDYLHTMGGRFLGLSHRFASRQDVL
                     EAARRFNEIGAACRKEDIQFLYHNHNWEFTKFDGQSAYDLLLDATDPELVKMELDTYW
                     VQRAGEDPVDYLNRLHDRCPLLHIKDMEAGDEQFFAEVGEGLLDWDGILRAADAAGTQ
                     WLVVEQDQCRRPALESITISYRNVQKMGIK"
     gene            complement(7779..8894)
                     /locus_tag="VGR29_11605"
     CDS             complement(7779..8894)
                     /locus_tag="VGR29_11605"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2074273.1"
                     /translation="MQRTKVGVIGCGQISGIYLRNCTRAFDNLEVVACADLVPELAER
                     RAREFGIPKACSVVELLGDPEIEVVLNLTVPHAHAGVNLQALQAGKHVYTEKPFALSR
                     EDADAVLDLARSRNLRVGSAPDTFLGGGLQTCRKLIDDGAIGTPYGASGLILMGNVYE
                     RLHLGTERYLELGWDPLFDMAPYYLTALVFLLGPVRTVTGFTESVLREITITNPASPR
                     HGETVAVPAPMHGAAALHFQSGALATVQAAKESFGYTPRLEVYGTDGILYAPDPNAFG
                     GPIRIHERNGAVRELPCECRFTEDSRGLGVADMASGIRTGGAYRCSGDLASHVLDITL
                     GIFESSRSGHHVEITAPEGLAAGLPAGHALLGVAGGA"
     gene            complement(8950..9213)
                     /locus_tag="VGR29_11610"
     CDS             complement(8950..9213)
                     /locus_tag="VGR29_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074274.1"
                     /translation="MPTIEVIKASEAPQAPKKLSKVADQLVTALNALRKDEVLKLSAD
                     EGKSLRGLKTSVGRITSNAGIKVTSWDDGTYLYVQKADQKSER"
BASE COUNT         1649 a         2872 c         2923 g         1885 t
ORIGIN      
        1 tcaccggagg atgttctttc gcgctctgcg ttgggaatac accatcatga gcctaccgac
       61 ctggctcctc ctcctttcag ggctcctcgc cgtggcggtg ctcctcggcg tcctcgcccg
      121 tcgagcccat atcccactca ccgtcgttct tgcattggtc ggctttctgg ctgggtggct
      181 cggagacatc gagagcccca tcagcgccga gcgattcgag gaggtcctgg ttttcgtctt
      241 cctgcccgtg ctcgtcttcg aagccgcctt cagcctttct acgcgtgctt tttttcgaaa
      301 cctcgtttcg atcctcgtcc tggcgattcc cgccctggtc atctcggcaa tatgcgtcgg
      361 gctctcactc tacggtttcc tcgatacacc gctgatcccg gccctccttt tcggagcgct
      421 catctccgct acggacccgg tggcggtggt ggccatcttt cgcgaactcg gagtgccccg
      481 gcgcctgctc acgctcgtgg aaggagagag cctgctcaac gatggcgtcg ccatcgttct
      541 tttcaccatc ctgctctccg gggcgctcgg tggcgatctc gcccctgccg aagggatgtg
      601 gaacttcctc gtcgtcttct ttggcggcgc tctcatcgga gcggcggtag ggcttctcgc
      661 cgcgctggca ctgccgtggc tggaccggct ttccgcggcc gcactttcca tggccgtcgc
      721 gtacggtggc tttgtcctcg ccgatgatgt gctggggttt tcaggtgtga ctgccactgt
      781 ggcggctggc ctggtgctgg gcgggtctgc cccaatccgc gcgaccgcgc cggtccgcga
      841 catgtgggaa agcctctggg acacgcttgg ctacgtcgcc aacgccctgt tgttcctgct
      901 gatggggctt gcgatcgacc cggctctgct gacggaaaac ctcggcgcga tcgggcttgc
      961 gatcaccgcc gtgttcgtgg cccgggctgt cgctgtcgtg ccactgatga cgctgctcga
     1021 gcgcgtcgcg ggtatcccgc cggtgggaat ccgcaacgag gcggtgctta tctggggcgg
     1081 cctgcggggc ggcgtcgcgc ttgcccttgc acttgcgctg ccggaggacc tcgctcagcg
     1141 ggaccagttc gtcgcgatga cgggtggggt ggtgctggcc acgctactgc tgaacgccac
     1201 gacgatcggc gcgctcgtcc gccgcttgag gctggatgag ccaagcccag ccgatcgatt
     1261 cctggcgggg ggagcacgcc tctccgccat tgccgcggcg cggacgcggc tcgttgacct
     1321 gcgtctcgaa gatccggtgg tcgtgtccga gttggaccat gccgaagagc tggcgcatgc
     1381 cgagttggcg cgtctttcgc ttgattccgt cgatgagatt cacgtgctga ttcgccgggg
     1441 cctggtcgtg gaacgggaaa cgtatcagcg actgagtgat gcgggcctgc tgccgccgac
     1501 tgccgctcgc ctgctgctgc atgaggtgga tgatcagatc gaagcgctgg gggtgggtcg
     1561 tgtcacgttg gccagcgtgt accagcgtga gcgtcccgcc tttgaccggt tgatggccag
     1621 attggcgagc tcattgccaa gcccgctcgg cgacgatcct ggctccgtcg cattcgcgga
     1681 agccagtgcc aggaggcttg cggcgcggcg caccagcgaa gccttcgagt tgttcacgcg
     1741 cctgccgaac atcggagcgg acacgatcaa ggaggccagg gcaacctttg cctggtggga
     1801 ggcggaagcg gaagcgcggc tgaccgaact ggacaccgac gtgacgcagg acatgcgtca
     1861 ggtgcagcat cagcaggccg agaaactcat ccacatggca tcgacggatg ctctcgaaga
     1921 ccttgccgag atcgggttgt tgccagcgag agtggcgcgc cgtgcggcgg agacggttgc
     1981 ggccgagatc ggcaacggtc ggagccaacc cggcggcgcc gtgcacgagc aatgaaggta
     2041 agtgatgggc gatgcgacga cccacatacc gctgaggtcc aggctttcgc gcgcctatgg
     2101 agtcctggtt ccggcttccc cgtgccatgc cgattccgga gcagatcgaa caggtggcag
     2161 agctgggcgt tccatgtgct ccgcatgtgg cggccggcat gtggtgacag gacattggtc
     2221 ggtgatgacc tacgcaccgc cccgtcccct gcacctggcg ggcggagcgc cagccagggt
     2281 tagtcaacct ggtagtagat gtccaggcta tggtgcaacg cccttgccaa tccgtaccgg
     2341 attgccatca tgatcggccc aggcagggat gcaccggttc cacgctggag atgttcccgc
     2401 gacgggaaca aggccctcac tcagggagga gtacgacgga atgtttgcag tcatctccct
     2461 cttggcaatt ctctcgctct ccttgcttgt cgtccggatc ggtgcgattg cgctgatgat
     2521 gacgggcatg tcgcgtgatg cggcggcatt ccaggcgtta tccgcgttct ccggtactgg
     2581 cttcactacc agcgaatcgg agctggcggt gactaccccg gagcgacgga agatcatcgc
     2641 tctgctgatc cgggcaggca gcgccggtgc catcacggta gtcacttcgt tgattctctc
     2701 attcgtcggc tcggaggacc gagcgccaga tcgcctgctg atcatcgttg gcgggctggt
     2761 ggggctggtc acactgtccc ggagtgggtg gttcaaccgc atgctgacgc caatcatcca
     2821 gcgagccttg agccactcaa cgccactggt attgcgcgat tacgctgcgc tgctccatct
     2881 gcgggaggat tatcgtgtag ccgagttcga ggttcgtccc ggggggtggt tggagggccg
     2941 ccaactgcgg gacctcgatt tcccggccga aggtctgctg atcctgggca ttgtgcaacc
     3001 ctccggggag tacgttggtg cccctccgac cgacttcgtg tttagctcgg gcgatacggt
     3061 cgtcgtctat ggccgtgaac cgaatctgga agaggtggcc cagcggcgat cgtcggatag
     3121 ccgggcacac gaggaaggga aagccgacca tgcaggggag gtcgatgcca agacgcacac
     3181 ggatatcgac caggatgctc accgctcccg gtgacaccgg gtaactggct cggaagcgct
     3241 tggagctgac ggaggctgag cgacccaatg ggacataacc gtggaggatg acacccggcg
     3301 cagggggaca gcgtcgacgg ggtcacgtac cgcggtaccc caggcaggaa tcgaatctgc
     3361 gtcctatcgc ttaaaaggca aaatggtcct aaagcagaga atttggtggc tagaccacct
     3421 ggaggcattc atttggactc actgcccgac cggggagtat acccgcgccg gtccacccaa
     3481 attggtgaaa aggccacttc gcaacagcct cgctggctct cggttcccat tttgagatcc
     3541 cgacgtctgc tggagggggc tcctatcccc agtcaagttg cgaggggccg gcgactccgg
     3601 ccatcctcac cggtttggga ccgtcgacaa cccggcgctg cacgggtgtc cttgcgccct
     3661 gctatacggt caccgctcgc tctgtcaggt ttggctgcat tggaagcgta accgtgaacg
     3721 tgctcccgta tccgggcgcc gactccacat cgatgcgccc gccgtgggcc tctacgatcc
     3781 gccgggagat atagagaccc aagcccaggc ccggcactcg accagtccct ccagcgacac
     3841 gatagaaacg ttcgaacaca tggggaagag cgtccggagg aatccctacc ccgtggtcag
     3901 ccaccgagat gcgcgcctct tgcatgtggg gagcaatgcg cacggtgatc ttgcttcctt
     3961 ctggcgagta cttgatcgcg ttcgtcaaaa ggttcgtcac cacttggccc agtcgctgct
     4021 gatccgctga caccatcaag gcgccctcag gcgcctcgac tcggataacc gattcatcgg
     4081 cgcccaacga ctcgacggct gcacgcgcta cggccgccag gtcggtctgc ccaagcacaa
     4141 gtgtcaggtg atctgcctgg attcgtgacg cgagcaggag gtcatcggtg agcctttgga
     4201 gcgtgtccgc ctgggcgatg atcgccgcaa cggctcgctc gttgtaagcc gcgcgccgct
     4261 gcatgagttg ggcatttgcc ctgatgccag tgaggggcgt acgcaattca tgggtcgtca
     4321 ccgccagaaa ttcgtgctgc aatcgctcaa ctgcccgccg ttccgcgata tcccgcatga
     4381 ttaccgatac cgcgttcaga tttcccagaa ggtccgggtg gggtgtaaag ccggctgaaa
     4441 cctcgactgt gcgaccgtcc atgcaggagc ccaccatctc aagctgaacc gcatgcgcgg
     4501 acctgagttc ggcccagatg ccttgccacg gtgcgtgctc cttcaccggc gcgaggatct
     4561 ggcgcaggtc cttcccgatg atttcgtggg ctgggtatcc aaacaggcgc tcagcggcag
     4621 cattccaggt cgtcaccaca ccctcgtctg acatgctgac gatcgcgtcg ctcgacatct
     4681 tgaccatggc cgccaattgc tccgagctac ggcgcgatcg gatcagttcc tgctcataag
     4741 cacggcggtc ggtcgcgtcg aatatggcat gggcgatcag caccggacga ccatcatcag
     4801 gtgtacgttg ctgtgaattg acgaggactg gcagcggctc ccggccgcga cgtgccaggt
     4861 caaaggccgc cccttgcacg gatccctgca agcgtagaag cgggaagatc tgattctcgt
     4921 agaacagctt gccgggcacc gtcaggatgt cctggaaccg gcgcaccgcg atcaggtcct
     4981 cacgcacagc ccctatccag ttgagcaagg tgtcgttcac gcggaggatc atgccgtcca
     5041 agcccgtgaa cacgtagccg cacgggccct gctcgtacag gtcttcgaga ctcgcttgca
     5101 acagatcatt gctcacgttc atgccgcgac tgtcacctga tcccacatga cttctgggag
     5161 ccaaggaaga ctttgatggc ttcgatcgtt tcttccggcg cgctcagatg tgggcagtgg
     5221 ccggttgcat ccagcacaac catgtcactg tccgacaagt gccgatgtac gtactcccct
     5281 acagccagtg gggcgaccgt atccgcatgg cattgcagga tgagtgagcg cacccgcgcc
     5341 ttcggcaggt cggcgcggtt atccgacagg aaggtcaccg ctgcgaactg cttgccaatg
     5401 tcggggtcgg tgcggcaaaa gctgttccgc aggttctcag cgagttcggg tcggtccgga
     5461 ttgcccatca tgaccggtgc caagctcgac gcccagccca ggtaattgct gtcttgcaac
     5521 tcaagcaagt cgtcgatatc ttcgcggctg aagcccccga agtagtcgcc gtcattgata
     5581 tagcacggtg aaggacctat gaggacgagg tgatcaaacc gttccggttc ttgaattgcc
     5641 gcgagcaggc caatcatggc gctgaccgag tgtccaacga agatgccgtg cttcagatcg
     5701 agttggtcac agatctccag gatgtcctgc gcgtagcctc taagcgagct gtatttggcg
     5761 cggtcatagg ccaagagatc ggagcggccg gccccgacat ggtcgaagag cactactcgg
     5821 tgggtgtcga cgaatgccgg gatgatcaag cgccacacgt tctgatcaca gccatatcca
     5881 tgtgcgaaca ttatcggcgt gtcgccgttg cccacgaggg taacgttgtt tctggtcagc
     5941 gcggacatcg taaatccccg acgagcgatc gattggcaag aattctgaca gttctccgga
     6001 tgtattgtat ccggccatgc cgcactgccg gacaggctgc tagaccaaaa gggcgtccgc
     6061 tatgctggca tgccgctact acctgctgaa cggttggttc acttcttcat gtcctgatcg
     6121 atcggctgtg ccatttgcgg cgtcacaaca tggtattgcc ctatacattc ggaattcgtg
     6181 atccgtcgcg ccgccaaatc tctcgtcgcg ccgagccacc cgctgaggct gaacctgcga
     6241 ctatggggct cagagcagga tcaggaggaa gttgagcatg gcagagtcaa atgtgaacgg
     6301 attttagtca cgattgcgat cagccagctt tttatctgtc gccatcgacg gggatctcga
     6361 tcgggctttc aaggacgaat gccacgtcct tcagccgggg agaggcccta cgcctcgtct
     6421 ggaaatcgac ctgcgagtat ggaggcattg agtcggcagc tggctggtac ccaaggcagg
     6481 aatcgaacct gcgaccatcc tcctagaagc cagtcgggcc cgttcaggca cgaaacatcg
     6541 gtagtaccgg ccgcctcatt tccagcgaac tcaccgccgt cgcggcgtct ttcatggcag
     6601 ccatcgtagt acggaaggac gtgggagtgc tcgcttaccc aagtccgcga ttgcatccga
     6661 tggttttcca ccgcaagccg aaaaatctcc cgttaaaaga gcagccagcc caggtacgag
     6721 agttcgtgcg acatggcgga ggtggtcgcg ttgcgccgga actcggcaat gacaagttac
     6781 aggcacgcgt cagtcgccag gatcggacat gccggcgaga gcgctggacg agcatttccg
     6841 aacagtgaca agcgggaggt tcgcatcagc tgcgtcacgc acgaggggag gtcaggcccc
     6901 agtcccgcag cctgactctc cactcgtgac cataccgacc tggtgatacc tgttaggtcc
     6961 tacttgatcc ccatcttctg cacgttacgg tagctgatcg tgatgctctc gagtgccggc
     7021 ctacggcatt ggtcctgctc cacgaccaac cactgcgtgc ccgcagcgtc cgcggcccga
     7081 aggatcccat cccaatcaag caggccttcg ccgacctcgg cgaagaattg ctcatcgccc
     7141 gcctccatgt ccttgatatg cagcagcggg catcggtcgt ggagcctgtt caggtagtcg
     7201 acggggtcct ctcccgcacg ttggacccag taggtatcca gctccatctt gaccagctca
     7261 gggtcggtcg cgtcgagcag gaggtcgtag gcggactgcc catcgaactt ggtgaactcc
     7321 cagttgtggt tgtggtagag gaactggatg tcctccttgc ggcacgcggc cccaatctca
     7381 ttgaagcgcc tcgccgcctc aagcacatcc tgcctcgacg cgaaccggtg ggaaagcccg
     7441 agaaagcgac cgcccatcgt gtgcaggtag tccacctgcg tgccgaggtc gtcggtcaac
     7501 tgctgcagcc cgccatggct tcccaccacg cgcaggccga tctgctccag gtgatgcttg
     7561 agctggtcaa ggctgatgtc cgccggcggg ggcccaagct cgacgccctc gtagccaatc
     7621 tcggcgattt tggtgagggt gccgagataa tcgccggcga gctcattgcg gacggtgtat
     7681 gcctgaagcg cgacgtgaaa ttccacgaga tgctcttctc tttcatcaag gtaccgcctc
     7741 tcggcgcgtg ttcgtaaaaa cgcatgccgc gtgaaaggtt acgctcctcc tgccactccc
     7801 aggagcgcat gaccggccgg cagccccgct gccaggcctt cgggggcggt gatctcgacg
     7861 tggtgccccg accgcgacga ttcgaaaatg ccgagcgtga tatcgagcac atggcttgcc
     7921 aaatcgccgc tgcaccggta ggccccgccg gtacggatcc cagaagccat gtcagccacg
     7981 ccgaggcccc ggctgtcctc ggtgaagcgg cactcgcagg gcagttccct gacggcgccg
     8041 tttcgctcgt ggatgcggat tgggccgccg aaggcgttcg gatctggcgc atagaggatg
     8101 ccgtccgttc cgtagacctc caggcgcggc gtgtagccga agctctcctt ggccgcttgc
     8161 accgtagcca gggcgccgct ctggaagtgg agggctgcgg cgccgtgcat gggcgccggg
     8221 acggcgacgg tctcgccatg gcgcggggac gccgggttgg tgatggtgat ctcgcgaagg
     8281 accgattcgg tgaagcccgt gaccgtgcgc acgggaccga gcaggaagac cagtgccgtc
     8341 aaataatacg gcgccatgtc gaagaggggg tcccaaccga gctccaggta gcgttcagtg
     8401 ccaaggtgga gtctttcata cacgttgccc atgaggatca agccgctggc gccgtatggg
     8461 gtaccgatcg ccccgtcgtc gatgagcttg cggcaggtct gcaaaccacc accgaggaag
     8521 gtgtcgggag cggagcccac acgcaggttt cgggagcgtg cgagatcgag gactgcgtcg
     8581 gcatcctccc gggagagcgc gaacggcttt tcggtgtaga cgtgcttgcc cgcttggagc
     8641 gcctggaggt tgacgcccgc gtgggcgtgg ggcacggtca gattcaggac tacctcgatc
     8701 tctgggtccc cgagcaactc cacaacgctg cacgcctttg ggatgccgaa ctcccgggcg
     8761 cgtcgctcgg ccagttcggg caccaggtca gcgcatgcga ccacctcgag attgtcgaag
     8821 gcgcgggtgc agttccggag gtagatgccg ctaatctgcc cgcagccgat gacgccgacc
     8881 tttgtcctct gcacggtcac cctcctcccg gatctcagtg atgctgtgat ctcttgcaaa
     8941 gcaagacgtt catcgttcgg acttctggtc ggctttctga acgtagaggt acgtcccgtc
     9001 atcccaggac gtaaccttta tccctgcgtt ggatgtaatc cggccaactg acgtcttgag
     9061 gccgcgaaga gacttgccct catcggcact taacttcagc acttcgtcct tcctgagagc
     9121 gtttagtgct gtcacgagtt ggtcggccac cttcgacaac ttcttcggtg cctgaggtgc
     9181 ttcggacgct ttgatgactt caatagtggg catggaactt cctctgattc atgatcgaat
     9241 agtacggttg ccatgatacc gccactagct gtgacgaaga gtgcctcgtc aagagtcgat
     9301 ggggccgggc ggtcctgatt cctggaacg
//
LOCUS       DASYWM010000285         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_370318, whole genome shotgun sequence.
ACCESSION   DASYWM010000285 DASYWM010000000
VERSION     DASYWM010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370318"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VGR29_11615"
     CDS             complement(<1..690)
                     /locus_tag="VGR29_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074275.1"
                     /translation="MSAVSVAAGSRSGIAVSRTRQNQLLRSLKRDPWASASLLFLLAV
                     VLAAILSPVLVPDTINSPSFGSRLLPPLTDGHIAGTDQLGRDVWGRMLLGARISMTVG
                     FLTVVLSALIGVPIGMLAGYYGGWLDVLLMRLVDVQLAFPFILLALTINAILGLGLRN
                     IIITLVIAGWIEYARIARGETLVVREREFVQAAHLTGARSGRIFLRHILPNIITPLIV
                     ISTLQVARFIIA"
     gene            complement(687..1604)
                     /locus_tag="VGR29_11620"
     CDS             complement(687..1604)
                     /locus_tag="VGR29_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074276.1"
                     /translation="MKGYVLGRLASGLIVIFLALTAVFFMLRLSGDPVLLFAPMDTSK
                     TNLEEIRERMGFNEPLAVQYATFMGDAIQGDFGESTRERRPAIEIVLERLPATLQLGG
                     LALVISLGVGIPLGILSATHHGSVWDKVARLIAVLGQAIPGFWLGVLLMLLFAVQLNW
                     LPTSGRGGVQHIILPAITLSALSTARYARLTRSTMLDVLGQDYMRTAKAKGLSNGTVL
                     WRHALKNASISLITMTGLEIGRLIEGAVVIEQVFAWPGMGRVTVQALLNRDFAVVMAA
                     VVLFAAMYTLANLVADLAYGWANPQVKLG"
     gene            complement(1638..>2592)
                     /locus_tag="VGR29_11625"
     CDS             complement(1638..>2592)
                     /locus_tag="VGR29_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2074277.1"
                     /translation="FKFKEWARGEYLLLERNEEYWRNDVMVQEVEFRFSPEFSTRLAA
                     LLAGELGVVKDIPVDSMQTVEESGTARIEAIPSSRINYVALVNNREDSVFNDVRLRQA
                     VNYAVDVNGIIEGIFQGEATRMAGPLSGLNPDVNPNLQPYEHDPEKARALIEEAGYAP
                     GELEITMDAPQGRYPMDTDAALAIAASLGDVGITVNVQYNEWGTHLDKIVNRQTGDMF
                     YLGWGPALDAMGTLAYLFVGDSTYSSYQNPEVEPLIEEATMTVDPEKRKELWFQVQQM
                     VYEDAAWLFLWQQHDIYGVANDISWAPRADEFLWMGETEAR"
BASE COUNT          539 a          779 c          786 g          488 t
ORIGIN      
        1 ggcaatgatg aaacgggcaa cctgcagggt actgatgacg atcagcggcg tgatgatatt
       61 tggcaggata tgccgcagga agatacgacc gcttctcgcg ccggtgaggt gcgcagcctg
      121 gacaaactcc cgctcacgaa cgacgagcgt ttcaccccgg gcgatgcggg catattcgat
      181 ccagccggcg atgaccagcg tgatgatgat gttgcgcaga cccagaccga gaatggcatt
      241 gattgtgagc gcaagcagga tgaacgggaa tgcgagctgg acatcgacca gccgcatcag
      301 caggacatcc agccagcctc cgtagtaccc ggccagcatc ccgatcggca ctccgatgag
      361 cgcggagaga acgacggtca ggaatccgac cgtcatggag atacgggcgc caagaagcat
      421 ccgtccccag acatctcgtc ccagttggtc ggttcccgca atatgcccgt cggtcagagg
      481 tggcaggagg cgggaaccaa agcttggact gttgatggtg tccgggacga gtaccggcga
      541 gaggattgcg gcaaggacca cggcgagcag gaagaggaga gaagcgcttg cccagggatc
      601 tcgcttcagc gatcgcagca gttggttctg tctcgtccgg gaaacggcga tcccggacct
      661 gctgccagcc gcgacggata cggcgctcat ccgagtttca cttgtgggtt cgcccaacca
      721 taggccagat cggccaccag gtttgccagg gtatacatcg ccgcgaagag gacgacagcc
      781 gccatgacca cggcaaaatc cctgttcagg agcgcctgga ccgtgacacg acccattccg
      841 ggccacgcga acacctgctc gatcaccacc gcgccctcaa tgaggcggcc gatctccagc
      901 cctgtcatgg taatgagcga gatactggcg ttcttcagcg catgccgcca aagcaccgtc
      961 ccattggaca atcccttcgc tttggcagtc cgcatgtagt cctgccccag cacgtctagc
     1021 atggtcgatc gcgtgagacg cgcgtatcgc gcggtgctca gcgccgacag ggtaatggct
     1081 ggaagaatga tgtgctgcac acctcctcgg ccacttgtcg gcagccagtt cagttgcacc
     1141 gcaaagagca gcatcaggag gacaccgagc cagaaccccg ggatcgcctg cccaagcacg
     1201 gcgatgagtc gcgcgacctt gtcccatacc gaaccatgat gtgtggcgga gagaatgcca
     1261 agcgggatgc ccacaccgag agagatcacc agcgccaacc ccccaagttg gagcgttgcc
     1321 ggcaagcgct caaggacgat ctcgattgcc ggacgtcgct cgcgggtcga ctcgccgaaa
     1381 tctccctgga tagcgtctcc cataaatgtt gcgtattgaa cagcgagggg ttcgttgaac
     1441 cccatccgct cgcggatttc ctccaggttg gttttggaag tgtccatcgg tgcaaagagg
     1501 agcaccgggt cacctgacag ccgtagcatg aagaagacgg ctgtcagggc caggaagatc
     1561 acaatcagac cggaggcgag acgcccgagg acgtagcctt tcattcagtc ttccccagat
     1621 ggtgatgagg aacggaacta tctcgcctcg gtttcgccca tccagaggaa ctcgtccgcg
     1681 cgaggagccc agctgatgtc attggcaaca ccgtagatgt catgctgctg ccacaggaac
     1741 agccaggcgg cgtcttcgta gaccatctgt tgaacctgaa accagagctc ctttcgcttt
     1801 tcggggtcga cggtcattgt cgcttcctcg atcagcggtt caacctccgg gttttgatag
     1861 ctgctgtagg tggagtcgcc gacaaagaga taggcaagcg tgcccatcgc gtcgagcgcg
     1921 ggtccccagc caaggtagaa catatcaccc gtttggcggt tgacgatctt gtccaggtgc
     1981 gttccccact cgttgtactg gacattgacc gtgatcccga cgtctccgag cgaggcggcg
     2041 atggcgagcg ccgcgtcggt gtccatcgga taacggcctt ggggagcgtc catggtgatc
     2101 tcgagttcac cgggtgcgta ccccgcttcc tcgatcaggg ctcgagcctt ttccgggtca
     2161 tgttcatagg gctgaaggtt cgggttgaca tccggattca gtcccgacag cggtccggcc
     2221 atcctggtgg cctctccctg gaaaataccc tcgatgatgc cgttgacatc gacggcgtag
     2281 ttcaccgcct gccggagacg aacgtcgttg aagacactgt cctcgcggtt gttcacgagc
     2341 gcgacatagt tgatgcggga agatgggatg gcctcgatgc gggcggttcc gctttcctcg
     2401 accgtttgca tcgaatccac cgggatgtct ttcacgacac ccagctcacc ggcgagcagt
     2461 gccgcgaggc gcgtgctgaa ctccgggctg aagcggaact ccacttcctg caccatgacg
     2521 tcgttgcgcc agtactcctc attgcgttcg agaagcaggt actcgccgcg ggcccattcc
     2581 ttgaacttga ac
//
LOCUS       DASYWM010000286         5272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_427029, whole genome shotgun sequence.
ACCESSION   DASYWM010000286 DASYWM010000000
VERSION     DASYWM010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427029"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2754)
                     /locus_tag="VGR29_11630"
     CDS             complement(<1..2754)
                     /locus_tag="VGR29_11630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV2074278.1"
                     /translation="MPYTELHLHTAFSFLDGASLPEEMIAQAVAYGYESLAVTDHDGI
                     YGAMEFAQAARAAGITPITGAEITLDDGSHLTLLAESRIGYGNLCRLLTDSKHGRPPA
                     PVALEEDAPDASLATTSLDDYAAGVILLTGCRESQLAHAIDEDRLRDAERILATYIDW
                     FGRENVVVELQQNFVFGDTQRIAQLITLANRLGVPYAATGNVHYHQQDRYRLQDVLVA
                     IANRATLDTSHRERRPNAQFHLRTPEEMATIFAAWPDAIHTTEMIARRCAAFDLTRDL
                     HYSFPDYETGTGETADTFLARTSWEAFGERYTDPVLPKIEKARAQLQEELRLIKRHGL
                     AGFFLLYRDLLNLARDVAAEIRGREATGSRIFLPPGRGRGSAVSSIVCYLIGLSPVDP
                     LRHDLYVGRFLNDELPSVPDIDLDFPREIRERMIERVYEVYGADRVALVSAFSTYRLR
                     SAVRDVGKVLGLPLPDLDRIAKLSEPRSADSLSEELDRLPEYASRKDAPPWCYLIEIT
                     KQLSGFPRHVTQHVGGMIISATPLNEMIPIQPAAMDGRYICQWDKDSCEDARMIKIDF
                     LALGMLSLVEECLDLIDASGQPPIDLSRIDFNDPEVFRMIQEGDTVGVFQVESRAQIQ
                     MVRRTKPESLDDLVVQVAIVRPGPIVGGAVTPFVERRTNPDFKPFYDHPKLEKILKET
                     LGVILYQEQVLQVAEVIAGFSAGQADQLRRAMTRKRSHEAMESMRALFIAGAEGKDIQ
                     ADLANQIFDKLAGFADYGFPKSHAAAFGFLAYQSCWLKHYYPAEFLCALLNNQPMGFY
                     APHVLINDAKRHGIRVRRPDINASQLECTVEGRRTIRIGLSFIKGISEDAALRIAAER
                     QTHGPYQSLADLIRRVALRPDAQQNLIASGACDSFGLQRREMLWQIGLFISPKGFRNK
                     YTRT"
     gene            complement(2923..3204)
                     /locus_tag="VGR29_11635"
     CDS             complement(2923..3204)
                     /locus_tag="VGR29_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074279.1"
                     /translation="MVKNPGAATRPHHLRALNEPRSITVVAQEGRPLAIVEEQGQQTV
                     AVIQDTWIIQDEWWRQEIHRQYYRLLLNDGRLRTVYHDRVADTWYEQAY"
     gene            complement(3153..4346)
                     /locus_tag="VGR29_11640"
     CDS             complement(3153..4346)
                     /locus_tag="VGR29_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074280.1"
                     /translation="MAIACLRIPHMALRIALLDQPKLDGLPLILSNPESGRAVVIDAT
                     DEATAKGIRSGLTLREASALCPEGIVLMPNPVMESRVAQEILTQLEAISPLVEADEQE
                     QGCWYIDLTGLDRHFPTTTAATEQMIASVPPVLRARAGVATTKFTARVASDIAKPAQV
                     RPITKGGEQRFLANAPVSLLPFPPEMTHQLYRLGLATLGAVAALPAAKLAARFGPDGR
                     LAWELASGIDRRAVVQRPREEIVIEELTMPAPAISREMLMIGLRQLVNRAFSRKSLKG
                     RQARQVTLRAGLENRRSWERTMVLKEPSGASQMITALDLRLQALEMPGPIESVSLHIS
                     GIVSEIAHQQVLPTLRPRHSSSVTEAINQLKQRYGLSPLFRVVEVEPWSRIPERRHAL
                     ITFEP"
     gene            4591..5082
                     /locus_tag="VGR29_11645"
     CDS             4591..5082
                     /locus_tag="VGR29_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2074281.1"
                     /translation="MSETTTSGTISVKNFRESLLFVFDETFANVHGAFLDPNESLFPT
                     LEGISATDASRPVGVGCSSVAAQVNHLNFYFDVAFQYMRGENPGRQDWASSWAKTEVT
                     EEEWTELKEELRVRQRTLVELINGTTSWDNEEIVGGAFAAVAHTAFHLGQIRHSLCVF
                     RQG"
BASE COUNT         1082 a         1421 c         1695 g         1074 t
ORIGIN      
        1 tgtgcgtgtg tacttgttgc ggaagccctt cggtgagatg aagaggccga tttgccagag
       61 catctcccgg cgctgcaagc cgaaactgtc gcatgcgccg gaggcaatca ggttctgttg
      121 cgcatcggga cgcaaggcga cgcgacgaat gagatcggca agggactggt agggcccatg
      181 tgtttgtcgt tcagcagcga ttcggagggc ggcatcttcc gagatgccct tgatgaacga
      241 gagtccgatg cggatggtcc gacgtccctc gacggtacat tccagctggc tggcattgat
      301 gtcgggtcgg cgtacgcgaa tgccgtgacg cttggcatca ttgataagga cgtggggtgc
      361 atagaaaccc atcggctggt tgttaagcag ggcgcagaga aactccgccg gataatagtg
      421 cttcagccag catgactgat aggcgaggaa gccgaaggcg gcagcgtggc tcttggggaa
      481 gccgtaatcg gcgaagccgg ccagcttgtc gaagatctgg ttggcaaggt cggcttggat
      541 atcttttcct tctgcaccgg cgatgaaaag cgcgcgcatc gactccatcg cctcgtgtga
      601 gcgcttgcga gtcatcgcgc ggcggagctg atctgcctgc ccggcactga atccggcaat
      661 gacctcggca acctgaagca cttgctcctg atagaggatg acgcccagcg tctccttcag
      721 gatcttctcc agtttcggat ggtcgtagaa gggcttgaaa tcaggattcg tccgtcgttc
      781 cacgaagggt gtaacggcac cgccaacgat tggtcccggc cggacaatgg caacctggac
      841 gacgagatca tccagcgact ccggtttggt gcgccggacc atctggatct gggcgcggct
      901 ctccacctgg aagactccga cggtatcgcc ctcctggatc attctgaaga cctctggatc
      961 attgaagtca atccggctga ggtcaattgg tggttggccc gaggcgtcga tgagatcgag
     1021 acattcctcg acgagcgata gcatgccaag tgcaagaaag tcaatcttga tcatgcgggc
     1081 atcctcgcag gagtccttgt cccactggca gatgtagcgg ccgtccatgg cggccggctg
     1141 gatcgggatc atctcgttaa gcggcgtcgc ggagatgatc atgccgccga catgctgcgt
     1201 gacgtgacgt gggaaacctg agagctgctt cgtaatttcg atcaggtaac accagggcgg
     1261 agcgtctttg cgggatgcgt attccggcaa gcgatccaac tcctcggaga gactgtccgc
     1321 cgatcgtggc tcactgagct tggcgatacg gtcaagatcg gggaggggaa ggccaagtac
     1381 cttgccgaca tcacgcacgg cgctccggag gcgataggtg gagaaggcag agacgagagc
     1441 gacccgatcc gcgccgtaaa cctcgtagac gcgttcgatc attcgctctc gaatctcgcg
     1501 cgggaagtcg agatcgatat ccggcaccga tggaagctcg tcgttaagga agcggccgac
     1561 gtagagatca tgccgtagcg gatcaaccgg ggagaggccg atcaggtagc agacgatcga
     1621 gctgacggcg gagcctcgcc cacgtcccgg cggcagaaag atacgtgatc ccgttgcctc
     1681 acgtccccgg atctcggcgg ccacatcgcg ggcaagattc aagagatccc ggtagagcag
     1741 gaagaatcct gccagaccgt ggcgcttgat gaggcgcaat tcctcctgga gctgggctcg
     1801 ggccttctcg atctttggta atacgggatc ggtgtaacgc tcgccgaatg cttcccagga
     1861 ggtgcgtgca aggaaggtat cggcagtttc accggtgccg gtttcataat caggaaagct
     1921 gtagtgcaga tcacgggtga gatcaaaggc cgcgcagcgc ctggcaatca tctccgtcgt
     1981 gtggattgca tccggccagg cggcgaagat cgtggccatc tcctccggtg tgcggagatg
     2041 gaactgggcg ttggggcgtc gctcacggtg cgaggtgtcc agtgtggcac gattggcgat
     2101 cgcgacgagt acatcctgca atcgatagcg atcctgctgg tggtagtgga cgttgccggt
     2161 ggcggcgtaa ggaacaccga ggcgattggc gagggtgatg agctgggcaa tgcgttgggt
     2221 atcaccgaac acgaagtttt gctgcaattc gacgacaaca ttttctcgac caaaccagtc
     2281 gatatacgtg gccaggatcc tttcggcatc acgtaggcgg tcctcatcga tggcgtgcgc
     2341 gagttggctc tcccggcagc cagtaagaag gatcacgccc gcggcatagt cgtccagtga
     2401 tgtggtggcc aagctggcgt ccggggcgtc ctcttccagc gccactggag caggtggacg
     2461 gccgtgcttg ctgtcggtga gcaagcggca gaggtttcca tagccgatac gggattcagc
     2521 aagaagcgtc aggtgcgagc cgtcgtcgag cgtgatctcg gcgccggtaa tgggtgtgat
     2581 gccagcggca cgtgcggcct gcgcgaactc catggcgccg tagatgccgt cgtggtccgt
     2641 taccgccagg gactcgtatc cgtaggctac ggcttgggca atcatctcct caggcaggga
     2701 cgccccatcg agaaaggaga aggcggtatg gagatggagc tcggtatagg gcataggagt
     2761 cccggaatat gcccttcggg cattggattc gactggtgtt cgcgacaagg cttacccacc
     2821 tgtggtcccg cgcttcgccg tgaagggcaa ggaaggtgta agccgacgcc ctgcctttac
     2881 tgaagctgtt ggaagagatg tcagacggga accgggagat ccttaatatg cctgctcata
     2941 ccaggtatcg gcgacacgat cgtggtagac agtgcgaagc ctgccgtcgt tgagcaacag
     3001 gcggtaatac tgccgatgaa tctcctgcct ccaccattcg tcctggataa tccaggtgtc
     3061 ctggatcacg gcaacggtct gttgcccttg ctcctcaacg attgcgagcg ggcgtccctc
     3121 ctgagcgaca acggtgatcg accgtggctc attcagggct cgaaggtgat gagggcgtgt
     3181 cgccgctccg ggattcttga ccatggttcc acctccacaa cgcgaaagag tggtgagagt
     3241 ccgtagcgct gcttgagttg gttgattgcc tcggtgaccg atgaggaatg gcgtgggcgc
     3301 agtgtcggta gtacctgttg gtgcgcaatc tcactgacga tgccactgat atggagtgag
     3361 acgctttcga tcgggccggg catctccagc gcctgcaaac ggagatcgag cgcggtgatc
     3421 atctgggatg cgccggatgg ttctttcagc accattgtgc gctcccatga ccggcggttc
     3481 tcgagccccg cccgaagggt gacctggcgg gcctggcgcc ctttaaggct cttgcggctg
     3541 aaggcgcgat tgaccagctg ccggagtcca atcatcagca tctcccggct gatggccggt
     3601 gccggcatcg tcaattcctc gatgacgatc tcctcccgag gtcgctgaac aacggcccgg
     3661 cggtcgatgc cggaggcgag ttcccaggcg agccggccat ccggcccgaa acgggctgct
     3721 agctttgcgg cgggaagggc agcaacggca ccaagcgtgg cgagtcccaa ccggtagagt
     3781 tggtgcgtca tctccggtgg gaaaggcaac aaggagacag gagcattggc gagaaaacgc
     3841 tgttcgcctc cctttgtgat gggtcgcacc tgcgctggtt tcgcgatgtc ggaggctacc
     3901 cgggcggtga atttggtcgt ggcgactccg gcgcgggctc tcaggacggg gggaaccgag
     3961 gcaatcattt gctcggtggc agcggtggtt gttgggaagt gacgatcaag cccggtgaga
     4021 tcgatgtacc agcaaccctg ttcctgttca tcggcctcaa ccagtgggct gatcgcttcc
     4081 aactgcgtca ggatctcctg ggcaacacga ctctccatga ccgggttcgg catcaggaca
     4141 atgccttcgg gacagagtgc ggatgcttcc cgcagcgtca ggccagagcg tatccctttg
     4201 gctgtagctt catcggttgc gtcgatgaca accgcgcgtc cgctctcagg gttactgagg
     4261 atgagcggaa ggccatccag tttcggctgg tcgagcaggg caatgcgtag cgccatgtgc
     4321 ggtatgcgaa gacaggcaat ggccatagat accctccatg atgaaaagcg cgtcctcaac
     4381 gtcgagaatg ggcggggtag ggagagcggg aggtccattc agttcgacgt gaacccagag
     4441 tagaacatat gttctactct gacaagatgt gacgcaaggt cacatcttgt agaggatcaa
     4501 tgggatacac gaaacgcgga caggaattgt ccaatcccat cgctaagatg aaacttcgtg
     4561 agttcgctgg cttttagaga ggaaagatcc gtgagcgaga ctacaacttc aggcacgatt
     4621 tcggtgaaga acttccgcga gtcactgctc tttgtcttcg acgagacatt cgccaatgtg
     4681 cacggcgcct ttcttgaccc gaacgaatca ctctttccaa cgctcgaggg aatctcggcc
     4741 accgatgcgt cgcgcccggt tggtgttggc tgctccagtg tcgcggcgca ggtgaaccac
     4801 ctgaacttct atttcgatgt cgcctttcaa tacatgcggg gcgaaaatcc cggcaggcag
     4861 gattgggcct ccagttgggc gaagaccgag gtgacggagg aggagtggac cgaactcaag
     4921 gaagagttga gggttcgcca acgtacattg gtcgagctga tcaacggcac gacatcctgg
     4981 gacaacgagg agatcgtcgg gggagccttt gccgccgtgg cgcataccgc ctttcacctg
     5041 gggcaaatcc ggcattcgct ttgtgttttt cgccaaggct aacccccaac ggggaccttg
     5101 tcagtatcgt acgtacaaca cgttcaaaga tgcagtacgt actccaaact ctctgaataa
     5161 aaatccccat cccctcaata tatgctcata accgcttgat agtggagcag gggacctcta
     5221 tagttaaaaa ctctggggga ttcccgcaga tgggcgggat cattgagggg at
//
LOCUS       DASYWM010000287        12607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_126563, whole genome shotgun sequence.
ACCESSION   DASYWM010000287 DASYWM010000000
VERSION     DASYWM010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 12607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12607
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126563"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(561..1418)
                     /locus_tag="VGR29_11650"
     CDS             complement(561..1418)
                     /locus_tag="VGR29_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEV2074282.1"
                     /translation="MTTERSRPSALTVGVVQTNSQDDKGANIAEALAGIDRAARMGAR
                     FVTLPETWTHLGPGKGDHAAAELIPRDLTRLLADKAREHTIWLHAGTIHERVPEHDKL
                     YNTTVVFDPSGDIVARYRKMHLFDVDITGHAAYRESDTIEPGEEMVTFDLDGVRIGLA
                     ICYDIRFPELFRILALRGAEIIMLPSAFTLMTGKDHWEPLIRARAIENSVFMVAAAQV
                     GSHPPNRMCYGRSMVVDPWGIVLAQAGDIPTVVTATLELEELARVRAQIPSLRNRIPA
                     SYAWPDARE"
     gene            complement(1467..2237)
                     /locus_tag="VGR29_11655"
     CDS             complement(1467..2237)
                     /locus_tag="VGR29_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV2074283.1"
                     /translation="MEAELNSMRNVAVRLAREAGRYAVAELEGNLGIEVKGNGSDVVT
                     RVDHEAERRIVAGIRDAFPDHAILGEESGRHGDRDAAVRWLVDPLDGTNNYVMDLSMF
                     GVCLTACLGDEPVVAVVHDSVRDITTSAIRDGGATRGERRLTLGEAPPLNRSTLSWTQ
                     GYAVSYDDPFRTRAMESMERATKRVLRTWSPSIDWGLVAAGHIGAFVAYRNEVWDLLG
                     GALIVQEAGGVVHHAPDYDCVIAGHADTVLELRSLLGV"
     gene            2327..2704
                     /gene="crcB"
                     /locus_tag="VGR29_11660"
     CDS             2327..2704
                     /gene="crcB"
                     /locus_tag="VGR29_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV2074284.1"
                     /translation="MEFFWIGIGGFLGANARYFLGHAITQRLGTLFPYGTLIVNLTGA
                     FLIGVIFTVLTDRVVDDRFWRHLVVTGFLGGYTTFSSYTYEAIRLMQEGRWSTTLLYV
                     VGSNILGLLACFAGVWVTKAISA"
     gene            complement(2708..3730)
                     /locus_tag="VGR29_11665"
     CDS             complement(2708..3730)
                     /locus_tag="VGR29_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV2074285.1"
                     /translation="MSNHPTISGEHHHGNAEGSPEHANRRPLAIALLITGLFLVVEII
                     GGLITGSLALLADAGHMATDVAALALSLFAIWLAQRPATPERSYGFARAEVLAALLNA
                     ATLVAISIYIFWEAFSRIGSPPEVDSGPMLVVAAAGLGANAVSAWVLMRGGGHKHNLN
                     TRGAFLHVVGDMLGSVGAIVAALVMLATGWYLADPILSAGIGLLILWSSWQLLRESVE
                     VLLEATPKGIDAMEVRAAMAQVEGVSGVHDLHIWTVTSGLIALSAHVEIAAGRDWHQL
                     LDQCTLPLREHFGIAHVTLQPEILDGSTRRFDGCSLDTEEGRNACRMALPARSLNPRH
                     GQPHQH"
     gene            3887..5104
                     /locus_tag="VGR29_11670"
     CDS             3887..5104
                     /locus_tag="VGR29_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEV2074286.1"
                     /translation="MSSPTRPSSLATSDSGYGMNRRQLIGRLAAAGFSAPVIASILQK
                     SAFAQDASPAATPTGEEILAGVGKDQDLIQQGTTTFEMPMDGYDEFLTPNDLFFIRSN
                     GPLFVDIDPTEWRLKVTGLVNEELELSLADLQSMEQRTITAFLECSGNTRGHFGKEPD
                     QVEGTQWGNGAVGNAEWTGVSLIDVLDKAGVKQGAIDVVSQGGDFDDMQRGLPIEMAY
                     DPDVMLVWQMNGEDLPKPNGGPVRLLVPGWGGIASTKWIVGLDVINQPFQGHFNVESY
                     VVVNEDGTILRPVREMPVKSVITIPGTGSTLDAGSQTISGFAWSGFGGITKVEVSVDG
                     GTDWEEATITEEAGRTSWVRFEHPWDAIPGEHTLRSRATDDRGLQQPLDVPWNARGYG
                     FNAIYEVPVKVNE"
     gene            complement(5162..6040)
                     /locus_tag="VGR29_11675"
     CDS             complement(5162..6040)
                     /locus_tag="VGR29_11675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV2074287.1"
                     /translation="MPGDLWRPDGPNIVVHGNNVDVLPLLPDAAFRLIYIDPPFNTGR
                     MQARQAMKTVRSPTGTRVGFKGQTYETIKGTLFGYNDDFEDYWAFLEPRLEEAWRLLT
                     DDGTLYLHLDYREVHYAKVLLDALFGRECFLNEIIWAYDYGGRSRRKWPSKHDTILVY
                     VKDPKQYYFDSDAVDREPYMAPGLVTPEKAALGKLPTDVWWHTIVSPTGREKTGYATQ
                     KPEGVLRRIIQASSAPGDWVLDAFAGSGTTGAVASQLGRSFVLVDSHPDAIEVMRQRF
                     SDDATVRFVPLAGSSA"
     gene            complement(6080..6304)
                     /locus_tag="VGR29_11680"
     CDS             complement(6080..6304)
                     /locus_tag="VGR29_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074288.1"
                     /translation="MSDQNGSSKRVIRVPEPTVVDPGSREYRDIEDVDVLVSPRDIGS
                     ASRSCLAIIIILLLIVLLICLFLLAQPLVD"
     gene            complement(6301..6915)
                     /locus_tag="VGR29_11685"
     CDS             complement(6301..6915)
                     /locus_tag="VGR29_11685"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV2074289.1"
                     /translation="MISERIGTWVRRYMLHVGAWFASVGITPNMATLIGLALNGIVAV
                     IIAFGYPVLGGVLLLFASAFDMVDGAIARSTNAVSKFGGFFDSTIDRYSEIVVYIGLL
                     VWLNQTGDDHLGSVLTLVAATGALMISYARARAEAIGYKASVGLVARPERVVLLAICL
                     IINQPLWALWVLAVLTHVTAAWRIIHVYRLAIADGGVSTTNRTP"
     gene            complement(6970..8115)
                     /locus_tag="VGR29_11690"
     CDS             complement(6970..8115)
                     /locus_tag="VGR29_11690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2074290.1"
                     /translation="MKIALVSPYSMHKPGGVAEHVAHLRDEFRRLGHTVKVMAPRGPR
                     GGLEVSEDEYGIGRTITIPGNGSKMRLTFDVTLYADVKAIMRREQFDIVHLHEPLMPV
                     LPYMVLLNSSSANVATFHAYSPSQPWYNAFKPYMSFVLGRIDARIAVSPPAKDFINRY
                     FEGDYQVIPNGIDVEAYGPHVAPFPWASDGCKRILFVGRFEEARKGFKYLLRALPMVK
                     QQFSDARLIVLGAGKPEKFEGLMERLGITGVEFKGFVSHEEKARYYASCDVACVPSTG
                     NESFGYVVVEPMAAGKPVVASNIAGYASVMTSGKNGVLVEPRNPQALALALVRVLADR
                     DLQQRLGATGLVDAQQYAWPRVAARVLEVYREATARAGSASWRQEFR"
     gene            complement(8318..9328)
                     /locus_tag="VGR29_11695"
     CDS             complement(8318..9328)
                     /locus_tag="VGR29_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV2074291.1"
                     /translation="MSTTTELTSTDTSARMTDGMTDADGEVEHQQVFPGATRGFLPEL
                     AALAVVIVWGSTFTLTKSTYTEIAPLAFGFARFIIIPVIAFAVLIIQARRTDQPNWWR
                     IKRQDLPLFVLTGVCGYTFYQLGFMLGLAHTSPFSGSLMISLQPIVTLGIVTLLGERQ
                     PLLVWVGVLVSLAGVAMFLANGDGESQLLGNVISFAGGASFAVYQVFNRRLIRDYPPT
                     TYSAYSTLFGAIPLLVICTPGAMRQDWGAISTQSWLTLAYMCVFPVYLAYIVWGWVIG
                     KRGVAISGLTLLVPVVAGVIAVLFFNEAFGPQKLFGGALALAGLLVVQQANRLAQTRR
                     AA"
     gene            9887..10888
                     /locus_tag="VGR29_11700"
     CDS             9887..10888
                     /locus_tag="VGR29_11700"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV2074292.1"
                     /translation="MSSGHWIGIDVSKARLDYCCGSSGKPASVANTPAGHARLLRVLR
                     TREIARIVLESTGAYHLAIVATLQDAGLPVSVMTPQLIKWYRESFGRKAKTDPKDARL
                     LAAFGEARRPYPSRVPTANERVLRELVARRDDLVALIGAEKNRLLVARDPRIRHHIAA
                     SLADAETRRAEIEREIDHLITGDPLLAKRRAQLRTVPGVGWVTSIVLLAYLPELGEMD
                     RRQIAALAGLAPYADDSGTPHKARHCSGGRSQVRRAMYLAAMTCVTHARVKRTVYRDH
                     YHEMSPAKGAKSTLVAIARRMLVLINAMVRDDLTWDDTDIGRGLHARPVPRRTMAPA"
     gene            complement(10995..12530)
                     /locus_tag="VGR29_11705"
     CDS             complement(10995..12530)
                     /locus_tag="VGR29_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2074293.1"
                     /translation="MIELEFLGTGTSTGVPVIGCDCIVCRSGDPLDARLRASVVIRAD
                     DVTLLVDTSPDLRYQMLRSGTRRIDGVLFTHTHADHTAGMDEIRRFNAMQRARIPAWA
                     PENAAADLQERFGYAFREEFPVFGMAPDLDLHVIRDTAPFNVAGVTVQPVPIMHGRLP
                     VLGYRVGNIAYLTDVKTIPEASRDLLRDLDVLVLTSLRLDQHPAHMTLEEALAAIDDL
                     KPRQAYLSHIGHDMGLHADVQAGLPKNVYLAYDGMRVGGPDRIAVISDIHGNMTAYDA
                     VLADIDARGIQRVINLGDVIGKGPGGSAAIARTRERCEATVRGNWDEFIHSKGDEPNT
                     ALKWWYDDLTEEDRAWLRSLPFSIDLRMSGHMVRLFHASADSVFHRVRAQHTDEDFDQ
                     MFRNTEATGNGDKPSVVVYGDIHAAYAEERGNKTLINAGSVGNPLDEPTASYVVLEGV
                     TDGVGAEPFRYEIVRVPYDIEAEISIARERGMLELDEYAVELRSALYRGLQSSKVATS
                     GPD"
BASE COUNT         2582 a         3929 c         3721 g         2375 t
ORIGIN      
        1 atcgtgaccg acccggaaac aacggtcgca tcggtcatcc acatggccgt cgaggttgaa
       61 ccggaagtgg aagccgaagc cgaggaaggt gcagaggccg aagctgccga gggcgaggaa
      121 tccgccgaag gcggcgagga agcatcctca gacgaggagt agacttcttc ttctgcagtc
      181 gatcagcatg cgaaaacggg ccagcggtga aaatctccgc tggcccgttt tcgcattacg
      241 gattcgtttt ccagctgcat gagcctaaaa ccggctctcc gtactattgg cggatatctc
      301 gtcccacaat cttcgggctg gtttcgggcg cacaaaaagg ccggcccaca cgggccggcc
      361 tttttgcttt gggcatcacg gctgtttcgg gcgctaggcg cgcgaaacct ccaccgtgat
      421 gcccgtacaa tcgctactca tgggcatcac ctcctttacc ctaatcggtt ggagttgtga
      481 gcgtccggtc ggcggtgctc ccgacctcgt gggaacttta acacgcacat ccatccagcg
      541 tcaacagaat ctggtgtgat ctattctcgc gcatccggcc aggcgtaaga tgccgggatc
      601 cggttcctga gtgacgggat ctgtgcccgc acacgcgcca actcctccag ctccagggtt
      661 gccgtgacca ccgtcgggat gtctcctgcc tgcgccagca caatgcccca cggatcgacc
      721 accatcgacc gcccgtagca catgcgattc ggggggtggc tccctacctg ggccgcagcg
      781 accatgaaga cgctgttctc gatcgcacgt gcccggatca gtggctccca gtgatcctta
      841 ccggtcatca aggtaaaggc cgatgggagc atgatgatct ccgcgccccg gagcgcgaga
      901 atgcggaaga gctcgggaaa ccggatgtcg taacagatgg ccagtccaat ccggacgccg
      961 tccaggtcga acgtcaccat ctcctcgccc ggctcgatgg tgtccgactc ccggtaggcc
     1021 gcgtggccgg tgatgtcaac atcgaagagg tgcatcttcc ggtagcgggc aacgatatct
     1081 cccgaagggt cgaacacgac ggtggtatta tagagcttgt catgctcggg cacccgctcg
     1141 tgaatggtcc ccgcgtgcaa ccagatggtg tgctcacgcg ccttgtcggc gaggagccgc
     1201 gtgaggtccc tgggaattag ctctgcagcc gcatgatccc ccttgccggg cccaagatgg
     1261 gtccatgtct caggcagcgt cacgaagcgc gcgcccatcc gtgccgcacg atcgatcccg
     1321 gccagcgctt cggcgatatt ggcccccttg tcatcttgcg aattcgtctg gacgacaccg
     1381 acggtcaggg cggacggacg ggagcgttct gtggtcatgg caatcctccg gatatcggga
     1441 tgtttgtccc atcatctcac gtgaacctag acgccgagca gtgaccgcag ctccaggacc
     1501 gtatctgcat gcccggcgat gacacaatcg tagtccggtg cgtggtgcac tacaccgcca
     1561 gcctcctgca cgatcagcgc gccaccgagg agatcccaga cctcgttcct gtaggcgaca
     1621 aaggcaccga tgtgaccggc agccaccaac ccccagtcga tggatggcga ccacgttcgc
     1681 aggacgcgct tcgttgcccg ctccatcgac tccatcgcgc gggtacgaaa gggatcgtcg
     1741 taggacacgg catagccctg ggtccaggag agcgtggacc ggttcagtgg tggtgcctcg
     1801 ccgagcgtca ggcggcgttc tccccgagtc gccccgccat cgcgaatggc tgaggtggtg
     1861 atatcccgca cgctgtcatg gacgaccgcg acaaccggtt catcgccaag acatgcggtg
     1921 agacagaccc cgaacatgga caaatccatc acataattgt tcgtgccatc cagcgggtca
     1981 accagccagc gaaccgcagc atcccggtca ccatgccggc ccgattcctc gcccaggatt
     2041 gcgtgatccg gaaatgcatc acgaattccg gcgacgatcc ggcgctcggc ctcatggtcc
     2101 acccgcgtta caacatctga gccgttgccc ttgacctcga tcccgaggtt gccctcaagc
     2161 tccgctaccg catagcggcc agcttcccgc gccagacgaa ctgcaacatt ccgcatggag
     2221 ttgagttcag cttccattgc atctccccca attgacgaca ttattcgacg gatgagcata
     2281 cgcgattccg tcgggtacac tcgggctgat gcggggaagg atgggcatgg agtttttctg
     2341 gatcgggatc ggtgggtttc ttggggcgaa cgcgcgctac ttccttggcc acgcgatcac
     2401 gcagcgactg ggaacactct tcccctacgg caccctgatc gtcaacctga ccggagcttt
     2461 cctgatcggc gtgatcttca cagtcctgac cgaccgcgtt gtcgatgacc gcttctggag
     2521 gcatctggtg gtgaccgggt tcctgggcgg atacaccacc ttcagcagct atacctacga
     2581 ggccatccgc cttatgcagg agggccgatg gtcaacgaca ctgctctatg tcgttggtag
     2641 caacatcctc gggctcctcg cctgcttcgc aggagtttgg gtgacgaagg caatcagcgc
     2701 ctgatattca gtgctggtgg ggctgcccat gccgaggatt tagggaacgc gccgggagcg
     2761 ccattcggca tgcattgcgc ccctcttcgg tatccagtga gcaaccgtca aagcgtcttg
     2821 tagagccatc gagtatttca ggctgcaacg ttacatgggc tatgccgaag tgctcacgga
     2881 gggggagcgt acactggtcg agcagctgat gccaatccct cccggccgct atctcgacgt
     2941 gcgcgcttaa ggcaatgagc ccggaggtta cggtccagat gtgcagatcg tgcacgcccg
     3001 agactccctc gacttgagcc atcgctgcgc gtacctccat cgcatcgatc cccttcggtg
     3061 ttgcctcaag aagaacctcg accgattctc gcaacaactg ccaggagctc cagagaataa
     3121 gcagcccgat accggcagag aggatcgggt cggccagata ccatcccgtc gcgagcatca
     3181 ccagtgccgc cacaatcgcg ccgaccgagc ccagcatgtc acccacgacg tgcaagaacg
     3241 ccccacgcgt attgagattg tgcttgtgac caccgccacg catcaggacc caggccgaca
     3301 ccgcgtttgc acccaggccc gctgcggcca ccaccaacat cggcccgctg tcgacctccg
     3361 gtggtgagcc aatacgacta aaggcctccc agaatatgta gatcgagatc gcgaccaggg
     3421 tcgcggcatt gagcagcgcc gccaacacct cggcacgggc gaagccgtag gatcgctcgg
     3481 gcgttgcagg tcgttgggcg agccagatcg cgaagagcga gagcgcaagc gcggcgacat
     3541 cggttgccat gtgcccagcg tctgccagca gggccagcga tccggtaatc aatcctccaa
     3601 tgatctccac gacgagaaag agcccggtga tgagaagcgc gatcgcgagc ggacgacggt
     3661 tcgcgtgctc aggggatccc tccgcattgc catggtgatg ctcgccggag attgttggat
     3721 ggttggacat acgggtttcc ttctcaggca ggctttcatc atcacagtac gtgcacgtgt
     3781 catgccgatg gatgccgccg tcaggagttc ccttctgcca gtttgggttt atactcgaag
     3841 aatcggacaa gcactattcg ttatattcag gagaagggga ttcctcgtga gcagcccaac
     3901 ccgtccttcg tcactcgcca caagcgactc gggatacggc atgaaccgcc gccaattgat
     3961 cggacggctt gctgcggcag gtttctcggc tccggtgatt gcctcaatac tgcagaaatc
     4021 cgccttcgcc caggatgcgt cgccagcggc cacgccgacc ggggaggaaa ttcttgccgg
     4081 ggtcgggaag gaccaggatc tcatccagca gggcaccacg acgttcgaga tgccgatgga
     4141 tggctacgat gaattcctga cgcccaacga cctcttcttc atccgctcca acggtcccct
     4201 cttcgtcgat atcgatccga ccgaatggcg ccttaaggtg actggcctcg tcaatgagga
     4261 acttgagctt tcgctcgctg atctccagag tatggagcaa cgcacaatca ccgcattcct
     4321 tgaatgctca gggaacaccc ggggccattt cgggaaggag ccggatcagg tcgagggaac
     4381 ccagtggggt aacggcgctg ttggcaatgc tgaatggacg ggtgtctcgc tgatcgatgt
     4441 cctggacaag gcgggcgtca agcaaggcgc aatcgatgtt gtctcccagg gcggagactt
     4501 cgacgacatg cagcgcggtc tccctataga gatggcgtac gacccggatg tgatgcttgt
     4561 ctggcagatg aacggcgagg atctgccgaa accgaacggc ggtcccgtcc gattgctcgt
     4621 tcccgggtgg ggcggcattg cgtcaacaaa atggatcgtc ggcctggacg tgatcaacca
     4681 acctttccag ggccacttca acgtggagag ctatgttgtc gtcaatgagg atggcacgat
     4741 tctacgcccc gttcgcgaaa tgcccgtgaa gtctgttatc acgattcctg gcaccggttc
     4801 aacgctagat gcaggctcgc aaacgatttc cggattcgcg tggtcgggct ttggtggcat
     4861 caccaaggta gaggtcagtg tcgatggcgg cacggactgg gaggaagcca ccatcaccga
     4921 agaagctggc cgcacctcct gggttcgctt cgagcaccca tgggatgcga tcccgggcga
     4981 gcacactctt agatcccgcg caaccgatga tcgcggcctg cagcaaccgc tggatgtgcc
     5041 ctggaatgcc cggggatatg gcttcaacgc tatctatgag gtcccggtaa aggtcaacga
     5101 atagtcgcaa cttgctgcgg gatcgagcgt tcgtcctccg accgaagggc gagcgatctc
     5161 cctatgcgct gctgcccgca agggggacga agcgcaccgt tgcatcatcg ctgaaccgtt
     5221 ggcgcatgac ctcgatcgca tccggatgtg agtcgaccaa cacgaagctc ctccccagtt
     5281 gactcgccac tgctcccgtg gtgccactcc ccgcaaacgc atccaggacc cagtcgcccg
     5341 gggcactact ggcctggata attctccgca gaacaccctc cggtttctgg gtcgcatagc
     5401 cggtcttttc gcggcccgtc ggcgagacga tcgtatgcca ccagacgtcc gttggtagtt
     5461 tccccagcgc cgctttctcc ggcgtcacga gtcccggcgc catgtagggc tcgcgatcca
     5521 ctgcgtcact gtcgaagtag tactgcttcg gatccttcac atagacgagg atcgtgtcgt
     5581 gcttggatgg ccacttgcgc cgtgagcgac cgccatagtc ataggcccag ataatctcgt
     5641 tgagaaagca ttcccggccg aagagggcat cgagcaggac cttggcgtaa tggacctcgc
     5701 gataatccag gtggaggtag agcgttccat cgtccgtgag caatcgccac gcctcttcca
     5761 gccggggctc cagaaaggcc cagtaatcct cgaagtcgtc gttgtagcca aagagtgtgc
     5821 ccttgatcgt ctcgtacgtt tgccccttga aaccgacccg ggtacctgtg ggagaccgaa
     5881 cggttttcat ggcctgtcgc gcctgcatcc tgccggtgtt gaagggggga tcgatataga
     5941 tcagccggaa ggcggcgtct ggcagcagcg gcaggacgtc gacattgttg ccatggacga
     6001 cgatatttgg gccatcagga cgccagagat caccgggcat acagtctgtc ctttccggat
     6061 cgagacctcg acacggggat cagtcgacga ggggctgcgc cagcaggaag agacagatca
     6121 gcagcacgat gaggagcagg atgatgatga tcgccaggca tgaacgcgat gcggacccga
     6181 tgtctctcgg gctcaccagc acgtcaacat cttcaatgtc ccggtactca cgcgatccag
     6241 gatcaacgac cgtcggttcc ggcaccctga tcacgcgctt gctggagccg ttctggtcgc
     6301 tcatggtgtc cgattcgttg tgctgacacc gccatcggcg atcgccaggc gatagacgtg
     6361 gatgatgcgc caggcagccg tcacgtgggt caggaccgcg agaacccaca acgcccacag
     6421 tggctggttg atgatgagac agatcgccag caggaccaca cgctccgggc gagccacgag
     6481 accgaccgat gccttgtagc caatcgcctc ggcacgggcg cgagcatagc tgatcatcaa
     6541 ggcacccgtc gcggcaacga gcgtgaggac ggaaccgaga tggtcatcac ctgtctggtt
     6601 gagccagacg agcaggccga tatagacaac gatctccgaa taacgatcga tcgtcgagtc
     6661 aaaaaagccg ccgaacttgg agaccgcatt ggttgaccgg gcgatggcgc catcaaccat
     6721 gtcgaacgca ctggcgaaca gcaacagcac tccgccgagg accgggtatc cgaaggcaat
     6781 gatcacggcc acaatgccgt tcagggccaa tccgatgagg gtcgccatgt tgggagtaat
     6841 gcccaccgag gcgaaccacg caccgacatg gagcatatat cgccggaccc atgttccgat
     6901 gcgctccgat atcaccgtcc cgtcccctct ccctgatcgc acccgctggc gaactaccgc
     6961 ttccccggcc taccggaact cctgccgcca cgaggcgctg cctgcccgcg cggtcgcttc
     7021 ccgatagacc tcgagcacgc gcgccgccac gcggggccat gcgtattgct gggcgtccac
     7081 caatccggta gcaccgaggc gctgctgcag gtccctgtct gccagcacgc ggacaagtgc
     7141 caacgcaagc gcctgggggt ttcgcggctc caccaacaca ccgttcttgc cggaggtcat
     7201 cacgcttgca tagccagcga tgttacttgc cactaccggc ttaccggccg ccatcggttc
     7261 caccaccacg tagccaaagc tctcgttgcc cgtcgaggga acgcaggcca catcgcaact
     7321 ggcatagtag cgtgcttttt cttcgtgcga cacgaacccc ttgaactcaa cgccggtgat
     7381 ccccagacgc tccatcaacc cttcgaattt ttccggcttg ccagcaccca ggacaatgag
     7441 cctggcatcc gagaactgct gcttgaccat cggcagggcg cgaagcaggt acttgaagcc
     7501 cttccgtgcc tcctcgaagc ggccaacgaa gagaatccgc ttgcacccgt cgctcgccca
     7561 cgggaagggc gccacatggg gaccgtaggc ctccacatcg atcccattcg gaatcacctg
     7621 atagtcaccc tcgaagtatc tgttgatgaa atccttggcc ggcggggaaa ccgcgattcg
     7681 cgcatcgatc cgccccaaaa cgaacgacat gtacggcttg aacgcgttgt accagggctg
     7741 cgacgggcta taggcatgga aggtcgcgac attcgccgaa gaagaattga gcagcaccat
     7801 gtagggaagc acgggcatca gcggctcgtg gagatggacg atatcgaatt gctcacgccg
     7861 catgatcgcc ttcacatccg cgtagagcgt gacatcgaag gtgagccgca tcttgctgcc
     7921 gttaccgggg atcgtgatgg tacgcccgat accgtactcg tcctcgctca cctcaagccc
     7981 gccgcgggga ccccgaggcg ccatcacctt gacggtgtgg ccgaggcgac gaaattcgtc
     8041 tcgcagatgg gcaacatgct cggcgacacc acccggttta tgcatgctgt acggtgaaac
     8101 aagggcaatc ttcacagggg tgattctagc aggttaggtc tgcgggaccg gatgcagaca
     8161 gtcacggcag gccgagggga agtgagcgaa cagacgctgg attccaacga atcaccaagc
     8221 ggccctccgg gcccgatccg ggtgggacaa tatcgaccgc gaagcatgac ctaacggaga
     8281 cagcagcatt gcgccgcggt ttcccttgct atgcacctta tgctgcccga cgcgtttggg
     8341 cgagccggtt cgcctgctgc acgaccagca gacctgccag ggcaagtgcg ccgccgaaca
     8401 gcttctgcgg tccgaaggcc tcattgaaga agaggaccgc gattaccccg gccacgaccg
     8461 ggaccaggag cgtcaggccg ctgatcgcca ccccgcgctt gccaatgacc cagccccaca
     8521 cgatgtaggc gagatagacg gggaagacgc acatgtaggc aagcgtcagc caggattgcg
     8581 tgctgatcgc tccccagtcc tggcgcatgg ctcctggcgt gcagatgacc agcaacggaa
     8641 tggcaccaaa cagcgtggaa tacgcagagt aggtcgtggg cggataatcc cggatcaggc
     8701 gccggttgaa tacctgatac accgcgaacg atgctccgcc agcaaagctg atcacgttac
     8761 ccagcaactg gctttcacca tcaccattgg ccaggaacat cgcgacaccg gcgagtgaga
     8821 cgagcactcc cacccagacg agaaggggtt ggcgttcacc cagaagcgtc acgatgccca
     8881 gcgtgacgat cggctgcagg gagatcatca acgagccgga aaacggtgat gtatgggcca
     8941 ggcccagcat gaagccaagc tgatagaacg tgtagccgca gacgccggtc aatacgaaga
     9001 gcggcagatc ctgccgcttg atccgccacc agtttggctg atccgttcta cgagcctgga
     9061 tgatgagcac cgcaaacgcg atgacgggaa tgatgatgaa ccgcgcgaaa ccgaacgcga
     9121 ggggtgcgat ctctgtatag gttgatttgg tcagcgtgaa ggtggacccc cagacgatca
     9181 cgacggcaag cgcagcgagc tccggcaaaa agccccgggt cgcacctggg aagacctgct
     9241 gatgttcgac ctcgccgtct gcgtcagtca tcccgtccgt catcctggcg gaggtgtcgg
     9301 tagacgtcag ctctgttgtt gttgacatga tggattacgc ctcaggcaca tatcgaaatt
     9361 ggtctcgcgc atttgtccta tcgaacctgg tgatgcctga cagcgtggtt ataacgatgt
     9421 tggaccgggt ttcacaacag gaaccctttt gttcagtcta ggggaaaagc gtgccatctt
     9481 ctgaccacat ttatccacgc cgcttcgcga ccctacagcg atttgcaatg agatagatac
     9541 ccctgatcgg ccaggtggta gagtgttgcg acccttgctg cagcaatagc cttccctaag
     9601 cttgtcgaat gggtcccgtg caacctgtca ggaggacatt gctgcgccgt ggcggcgacc
     9661 aagttgcatg cgccgcaagg cctctgcttg tatgttccaa ctgacatcga gccggcacct
     9721 gctcctccgc tgggtcttga cgaccgtcgc gtagtccagg tccggctcga tgagggtggt
     9781 tgcccccgac agtcgaacgg ttacatggac gccctccgca taacaagtac gacgttcggg
     9841 tgcagctcca gtgtaatccg cgtccgactc accaaggagc gcaacgatgt catcaggcca
     9901 ctggatcgga atcgatgtgt ccaaggcccg cctggactat tgctgcgggt cgtccgggaa
     9961 gccggcctcc gtcgcgaaca ccccagcggg acatgcccgc ctcctgcgcg tgctgcgcac
    10021 gcgggagatc gccaggatcg tgctggaatc gacgggcgcc tatcatctag cgatcgtcgc
    10081 cacgttgcag gatgccgggc tgccggtgtc cgtgatgacg ccacaattga tcaaatggta
    10141 tcgcgagagc tttgggcgca aggccaagac ggaccccaag gatgcccggt tgctggccgc
    10201 cttcggtgaa gcgcgtcgtc cttacccgtc gcgcgtccca accgccaatg agcgcgtgtt
    10261 gcgggaactc gtggcacgac gggatgatct ggtcgcactt atcggggccg agaagaatcg
    10321 gctcctcgtg gcccgggatc cccggatccg ccaccatatt gccgcctcgc tggccgatgc
    10381 cgagacccgc cgtgccgaga tcgagcgcga gatcgaccac ctgatcaccg gtgacccgct
    10441 cctggccaag cgccgggcgc aactccgcac cgtgcccggc gtaggatggg tgaccagcat
    10501 cgtgctgcta gcctacctgc cggaactggg ggagatggat cggcgtcaga ttgccgcgct
    10561 cgccgggctt gccccgtacg ccgatgattc cggcacccca cacaaggcgc gccattgttc
    10621 cggtggccgc agccaggtcc gccgcgcgat gtatctggcc gcgatgacct gtgtgaccca
    10681 tgcgcgcgtc aagcggacgg tttatcgaga tcactatcac gagatgtccc ctgcgaaggg
    10741 cgccaaatcg acgctggtcg cgattgcacg gcgcatgctg gtgttgatca atgcgatggt
    10801 gcgcgatgac cttacctggg acgacacgga catcggacgt ggtcttcacg cccggccggt
    10861 accccggcgg acgatggctc cagcgtgatg cccatccctt gacattcaag acggttacta
    10921 caacctacaa accttcctct ctgaaaacac cttaaaggca atctcccacc tggtgcgcat
    10981 gacacagtaa ggtttcagtc gggccctgac gtggcgactt tggacgattg caatccgcga
    11041 tacagtgcgg atcgcagctc aacggcgtat tcatccagtt caagcatccc acgctcccta
    11101 gcaatcgaga tctcggcttc tatatcgtat ggaacccgca cgatctcgta ccggaatggc
    11161 tctgccccaa ctccatcagt cacaccttcc agaacgacgt aggaggccgt cggctcgtcc
    11221 agtggattgc cgacactccc ggcgttgatc agcgtcttgt tccctcgctc ttcagcatat
    11281 gcggcatgaa tatcgccata cacgacaacg gaaggcttgt caccattacc agttgcctcc
    11341 gtattccgga acatctggtc aaaatcctcg tcagtgtgtt gagcccgtac ccgatgaaac
    11401 acgctatccg ctgatgcatg aaacagccgc accatgtggc cgctcatccg aaggtcgatc
    11461 gaaaacggca gggatcgcaa ccatgcgcgg tcttcttccg tcaggtcgtc gtaccaccac
    11521 ttcaaagccg tattcggttc atcgcctttc gagtggatga actcatccca attgccgcgg
    11581 actgtcgcct cgcatcgttc gcgggttcgg gcaattgccg ccgagccacc cgggcccttc
    11641 cctatcacat cgcccagatt gattacccgt tgaatgccgc gcgcatcgat gtcagcaagg
    11701 acggcatcat aggcggtcat gttgccgtgg atatcagaga tgaccgcaat ccggtccggt
    11761 cccccaactc gcatcccgtc gtatgccagg tacacattct tcggtaaccc ggcctggaca
    11821 tcggcatgga gccccatgtc gtgcccgatg tgggagagat aggcttggcg cggcttcaga
    11881 tcatcgatcg cggccagggc ctcttcgagt gtcatgtggg ctgggtgctg gtcaaggcga
    11941 agagacgtga ggaccagcac atcgagatcc cggaggaggt cgcgggatgc ctcgggaatc
    12001 gtcttcacat cggtgaggta ggcgatgttg ccgacgcggt accccagcac cggcaaccgg
    12061 ccatgcatga tcggcacggg ttgcaccgtc acgccagcca cgttgaaggg agcggtatcc
    12121 ctgataacgt ggagatcaag atccggtgcc atcccgaaga ccgggaactc ctcacgaaac
    12181 gcgtagccga atcgctcctg cagatcagcc gccgcgttct ctggggccca tgcgggaatc
    12241 cttgccctct gcatggcatt gaatcgccga atctcgtcca taccggcggt atggtcggcg
    12301 tgggtgtgcg tgaacagcac accgtcgata cgccgggtac cgctgcgcag catctggtag
    12361 cgaagatcgg gcgaagtatc gaccagcaac gtgacatcat cggcgcggat cacgactgac
    12421 gcccggagcc gcgcatccag agggtcgccg gatcggcaga caatacagtc gcagccaatc
    12481 acgggcaccc cggttgacgt gccggttccc agaaattcca gttcaatcac aaaggcttgc
    12541 tttcaccaag tgcggcaaga agaacgatga gagggaccgc cgcgttcctc ttcggatgag
    12601 tatacga
//
LOCUS       DASYWM010000288         3596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_351977, whole genome shotgun sequence.
ACCESSION   DASYWM010000288 DASYWM010000000
VERSION     DASYWM010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351977"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VGR29_11710"
     CDS             complement(<1..255)
                     /locus_tag="VGR29_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074294.1"
                     /translation="MPIIRMPANLIMPSQKTNRRNVIRGAAGLGLGIGAAGAFGNAFA
                     QDHNHATPGAGASGGDDIGSDASTESTGDATPAPVNAVTPF"
     gene            742..2508
                     /locus_tag="VGR29_11715"
     CDS             742..2508
                     /locus_tag="VGR29_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2074295.1"
                     /translation="MKRRTPGSPSVTSRAKLQVVIFSSLLVALQLLGMAGLAAAQDAT
                     PGASPVGEATELALVDIPMAPGSEAEGAVPDATVTFNLGGEIDTGDPQVMAFLNEIEI
                     ASKVWVPLLALNEENLPASAGAESATVSADGTVYTFTIRDGMTYTDGEPVTAGNYAYA
                     LKRACSPEVAGNYSNILYAITGCEAWRSADLAGETAGLEAALDESIVAVDDSTLEIHL
                     DYAAGYFPYVMTTWVTYPVREDLVEGNGADWWRDISNYVGNGPFKVVSHTDNQEWVFE
                     RNEDYFRGAPGIQTLVYKEVDSPETALLAYQQGEFDLIGPSSTQLPQVEADPVLSEQL
                     ARQSDASTYYMAFNNASAPFDDVQVRQAFSYAMNRQQYIDQIANGVGIPAGTFLYEGI
                     PGYQTDFQQSYDPEMAKQLLADAGYPEGEGFPVLQLYYNSESAASQQVATFWSQNFQQ
                     VLGVTVEPTPIDVAQLQSLRTNRDPSLLIWLGNWFEDYPHPQNWLSLVYGPGSTRAPL
                     GWDNQEYYDLVTEADRLPIEEAIPLYEQADALLAEQAPVAFYLHGESLVLVSPQLQGY
                     VSYPTSVVDTRYQIEKIYKVAE"
     gene            2647..3579
                     /locus_tag="VGR29_11720"
     CDS             2647..3579
                     /locus_tag="VGR29_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074296.1"
                     /translation="MARYLVTRILWMIPVLWLVATVTFVLMHSAPGSPWDAKSGASGR
                     DLDASLQGSFNRKYGLDEPILVQYLTYLKNAVTLDFGESFVKQDKPVIEVIGDGFPYS
                     ARIGLLALGLALGIGIPIGILAATRHNTAVDYVSLFFTTVSYTLPDFVIAIFLLIIFS
                     VQLGLMPVLFTDWRSYILPALALGTGSAAFIARLTRSSVLEAMRNDYVTTARAKGLSS
                     RAVMARHVVRNAMIPVITVIGPAFAGLITGTIIIERVFGVPGMGHLFIDSINSRDYPV
                     IMGTTLFYAFFITFANLLVDIAYGFADPRIRESR"
BASE COUNT          673 a         1093 c         1063 g          767 t
ORIGIN      
        1 gaacggcgtg actgcgttca ctggtgccgg ggtcgcgtcg ccggtggatt ccgtcgatgc
       61 atcggacccg atgtcgtcac cgccggaagc gcccgcgccg ggtgtggcgt gattgtgatc
      121 ctgggcaaag gcgttgccga acgcacctgc ggcgccaatg cccaggccga ggcccgcagc
      181 cccgcgaatg acgttgcggc gattggtctt ctgcgagggc atgatgagat ttgcgggcat
      241 tcgaataatt ggcatccgta aactcctttt gtgtcccgtg ggacgctctt ccccggtttt
      301 cggttatctg tcacggtctc cgtccggttt ccgggcggtt caatccgttg agggaagtgt
      361 cgtccggaag gcgtggcgcg ggagtctcag aagtggggat caccatgtca atttcatgac
      421 agttggccga atttggcaac tcattccctt ccatctgtgt agcttcgtgt gtccacaggc
      481 gtaggatacc tctggccgct ccgggtgctt acgcttcgcc gaattcgatc acgttggctt
      541 gacactgcga catgtctcac ctacggtagg ttgcacacat acgtaatatc cgcgcatgcg
      601 gaaggtacag gaacagggac gtgcctctat ggggcatccg tagcaactgg cagcagtgcc
      661 gttggaacgc gatgaccatg agcaacccgg tttctggcgt ttggcggcat gcccgcgcac
      721 cagtcgtaca aggaggatct tgtgaagcga cgaacacccg gctcaccatc tgttaccagc
      781 agggcaaagc tccaggttgt catcttttcg agtcttcttg tagcactgca attgttgggg
      841 atggcaggac ttgccgctgc ccaggacgca acacctgggg catcgccggt gggtgaagcg
      901 acagagctgg ctcttgtgga tattcccatg gctccgggta gtgaggctga gggcgccgtt
      961 cctgatgcca cggtcacctt caatctcggt ggcgaaatcg ataccggtga tccccaggtg
     1021 atggccttcc tgaacgagat cgagattgcc tcgaaggtct gggtgccact tctcgcgctc
     1081 aacgaagaga accttcccgc aagcgcgggc gcggagagcg cgacggtttc cgccgacgga
     1141 acggtctata cctttacgat ccgggacgga atgacctaca cggatggcga gccggtgacg
     1201 gcgggcaact atgcctacgc cctgaagcgt gcctgcagcc cggaggttgc cggcaactac
     1261 tccaacattc tctacgcgat cacagggtgt gaagcctggc gcagcgccga tctcgcgggg
     1321 gagacggccg ggctcgaggc ggcgctcgat gaatcgatcg tggccgtgga tgattccacg
     1381 cttgagattc atcttgacta tgccgccggc tatttcccct atgtcatgac gacttgggtg
     1441 acctatcctg tccgcgagga tctggtggaa ggcaacggtg ctgactggtg gcgcgacatc
     1501 agcaactatg tcggcaatgg ccccttcaag gtcgtcagcc acaccgacaa tcaggaatgg
     1561 gtcttcgagc gcaacgagga ttatttccgt ggcgccccgg gtattcagac cctggtctac
     1621 aaggaagtcg acagccccga gaccgcgctc ctcgcgtacc agcagggcga gttcgacctc
     1681 attggtcctt cctcgacaca gttgccacag gttgaggcgg atccggttct gagtgaacaa
     1741 cttgctcgcc aatccgacgc cagtacgtac tatatggcgt tcaacaacgc ctctgcgccc
     1801 ttcgatgacg tgcaggtgcg gcaggccttc tcctatgcca tgaatcgcca gcagtacatc
     1861 gaccagatcg ccaacggtgt tggcatcccg gctggaacgt tcctctatga aggtatcccc
     1921 gggtaccaga ccgacttcca gcagtcgtac gatcccgaga tggcgaagca gcttctggcg
     1981 gatgcaggtt acccggaggg cgagggtttc ccggtcctgc agctctacta caacagcgaa
     2041 tccgccgcgt cccagcaggt cgcgacattc tggtcccaga atttccagca agtgctcggc
     2101 gtgaccgttg agccgacgcc gatcgatgtt gcccagctcc agagcctgcg cacgaaccgc
     2161 gacccatcgc tcctgatctg gcttggcaac tggttcgagg actatccgca tccgcagaac
     2221 tggctctcgc tggtctacgg accgggcagc acccgcgccc cgctaggctg ggacaaccag
     2281 gagtactacg acctcgtcac cgaggccgat cgtctcccga tcgaggaagc gatcccgctg
     2341 tacgagcagg ctgatgcctt gttggcggag caggcacccg ttgcgttcta cctgcacggt
     2401 gagagcctgg tgctcgtgag cccgcagctc cagggctacg taagctaccc caccagcgtc
     2461 gtcgatacgc gttaccagat cgagaagatc tacaaggtgg cggaataacc acgaacagga
     2521 ttctgagacg gcaaggtgtc ccgatgagtg ggacactttg attcgaacat gagaagcggg
     2581 tcggctgggg cccgctttct caatcgcaat gcctccccga ggcgaccagc gtagggaaga
     2641 gcacgcgtgg cgcgttacct ggttacccgc atcctgtgga tgattcccgt tctgtggctc
     2701 gtcgccaccg taacgttcgt gctcatgcac tccgcgccgg gaagtccatg ggacgccaag
     2761 tcgggtgctt ccggccggga tcttgacgcc agcctgcaag ggtctttcaa ccgcaagtac
     2821 gggcttgatg agccgattct ggttcagtac ctcacctatc tcaagaacgc cgtgaccctg
     2881 gacttcggtg aatccttcgt caagcaggac aaaccggtca tcgaggtgat cggcgatggt
     2941 ttcccgtact ccgcccggat cggcctcctg gccctgggct tggccctggg aatcggcatc
     3001 ccgatcggta ttctcgccgc aacacgtcac aacactgccg tcgattacgt tagcctcttc
     3061 ttcaccaccg tcagctatac gctaccggat tttgtgatcg cgatcttcct gctgatcatc
     3121 ttttcggtgc aactcgggct catgccggtc ctctttaccg actggcgtag ctatattctg
     3181 cccgcactgg cactggggac cggcagcgcg gcgttcattg cccgcctgac acgctcaagc
     3241 gtgctggagg cgatgcgcaa cgactatgta accaccgctc gcgccaaggg gctctccagc
     3301 cgtgcggtga tggcccggca tgtcgttcgc aacgccatga tcccggtcat tactgtcatt
     3361 ggtccggcct tcgccggcct gatcaccgga accatcatca tcgaacgcgt cttcggtgtg
     3421 ccggggatgg ggcatctctt tatcgacagc atcaatagtc gtgattaccc ggtcattatg
     3481 ggcaccacac tcttctatgc cttcttcatc accttcgcca atctgctggt cgacattgcc
     3541 tacggttttg ccgatcctcg catccgtgaa tcacgctaac cagacaagga ggaggg
//
LOCUS       DASYWM010000289         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_370556, whole genome shotgun sequence.
ACCESSION   DASYWM010000289 DASYWM010000000
VERSION     DASYWM010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370556"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(422..700)
                     /locus_tag="VGR29_11725"
     CDS             complement(422..700)
                     /locus_tag="VGR29_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074297.1"
                     /translation="MERNDKQRAKRHHRHVEDDEIDRALGELRVPLSVMYGYSQLLQR
                     RINQGRIHEAYACLDGLAAIQRSARVMEANLMRIEAMARARQSDQEES"
     gene            1017..1397
                     /locus_tag="VGR29_11730"
     CDS             1017..1397
                     /locus_tag="VGR29_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074298.1"
                     /translation="MMRDQQRWAYCDVRPDGTITYYGIAEGHRIQARPNVHAVDTNRW
                     VEEAVAWLGWQGWELVSVVHGEGRTFYFRRPIAEHTAEVEAQNIGMEAVSLAVAEEDL
                     VLDADADARSINPARLIEEEFLPE"
     gene            1514..1984
                     /locus_tag="VGR29_11735"
     CDS             1514..1984
                     /locus_tag="VGR29_11735"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2074299.1"
                     /translation="MESILVKLFEHNQWANLRAVDASVHLTDAQLDATVQGTAGTVRD
                     TMMHIAGAEQRYVMRLSGRQPTYGERDGWPGVDTLRQAFNESGRALIELAGRADPDEV
                     LEGEYQGQPYKLPVSTIYVQAINHATEHRSQIATILTQHGIEPPDFSGWAWDRR"
     gene            complement(2578..>2787)
                     /locus_tag="VGR29_11740"
     CDS             complement(2578..>2787)
                     /locus_tag="VGR29_11740"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV2074300.1"
                     /translation="RNDDCIHYLISTTDEADAIWVTETWTTKDAHEASLESAEVRALI
                     GQAIPLMSRSTSDQFELQVIGGKGL"
BASE COUNT          537 a          814 c          889 g          547 t
ORIGIN      
        1 ctcgcctcat cgggcccgac gaccctgcac atgctcctgc gatcgcggct ctgcgcagca
       61 agtaccccca gtatcacgcc atggcaatcg agcagcggcc agtgatagtg atccagccat
      121 accggacgac atcctggggc atctgagtta tttatgagta atggcgtggc aggcgtgcca
      181 gctcccgtcg gcccagtgtc cgtccttgtc caatggctat ctgcagtccg tgaccctcgg
      241 gggaaaggaa caccgccacc tggatggcgg cgttcctgat ggtgcttata gacgcccaat
      301 ctggctcagg tctgtaggac ctccagcggc gttggaagat tgtcaccaac gacagggacg
      361 attgccagga gcgtcaggat cgttatggct cgcctggtta cgtctgtctg attcgcagca
      421 ttcaggattc ctcctgatcc gattgacgtg cgcgcgccat ggcctcaatt ctcatgaggt
      481 ttgcctccat caccctcgcc gatcgctgga tggccgccag cccgtcaagg caggcgtatg
      541 cctcatgaat gcgtccctga ttgatgcggc gctgaagtag ctgtgaatag ccatacatga
      601 ccgagagagg aacccggagt tccccaagcg cgcggtctat ctcgtcatcc tccacgtggc
      661 gatggtgccg tttggcacgt tgcttgtcat tgcgttccat ggttggtctt taccctcaca
      721 caggataaga gtcccccttc tgcgcgggaa cgtgcaatgc tcgtgccggg atcacatcgc
      781 agggaccatg cttcgttcca tggcgtcaaa aggcgtgaac atcccaaccg taaggggacg
      841 tcagtgtgag gcaccgggtg ccagtcacga ctccctgtgc ggagaagatc gtcgcggttt
      901 atagtggcct ccatgagaaa accgggcatc catcaggctt gaaactgtca cagggtgtga
      961 aaaggatcca caacgcctga ggccacgatg acatctgagc ggatgaggag acaggtatga
     1021 tgcgcgatca gcagcggtgg gcctactgcg atgtccggcc cgatggcacc atcacgtatt
     1081 acggcatcgc ggagggccat cgcatccagg cccggcccaa tgtccatgcc gttgacacca
     1141 atcgctgggt ggaggaagcg gttgcctggc ttggctggca ggggtgggaa ttggtcagcg
     1201 tcgtgcacgg ggaggggcgc accttttact tccggcggcc catcgccgag catactgccg
     1261 aggtggaggc gcagaatatt ggcatggagg ccgtcagcct ggctgttgcg gaggaggatc
     1321 tggtcctgga cgccgatgcc gatgctcgct cgatcaaccc ggcccggctc attgaggagg
     1381 agttcctgcc agagtaggga gaccggtagg ggcaagactc cttcctcggg acgcttctgg
     1441 gcggcaggag ttggggcaac gcgagttgcc ggaagacaag cggatagcca catccgcgca
     1501 cggaggggac gcaatggagt cgattctcgt caaactgttc gagcataacc agtgggcgaa
     1561 cctgcgtgcc gtcgatgcgt ctgtgcacct gaccgatgcc cagctcgacg ccaccgtgca
     1621 gggcacggcc ggcaccgttc gcgacacgat gatgcatatt gctggcgccg agcagcgcta
     1681 tgtgatgcgg ttgagcggcc gccagcccac ctacggcgag cgtgatggct ggccgggtgt
     1741 cgacacgctt cggcaggcgt tcaacgagag tggccgtgcg ctgatcgagt tggccgggcg
     1801 agccgatccg gacgaggtgc ttgagggcga gtaccagggc caaccctaca agctgccggt
     1861 ttcgaccatt tacgtgcagg ccatcaacca tgcgaccgag caccgctccc agatcgcgac
     1921 catcctgacc caacacggca ttgagccgcc cgacttctcc ggctgggcct gggacagaag
     1981 gtaatcgaga ggtaccgtgg attggtagcc ccgggtcgtg tggatccgga cgtgacatca
     2041 actaatctcg tgtggtgctg tatcctgatg cgcgcggtcg gtcgcctgtc gacgagaggc
     2101 tctcttatcg gagcacgaca gggataccaa tctattcccc cttcctccat ccacaggaag
     2161 acgccggacg ccctcttcgc atctcgatat cgttccagaa gtggtcctcg gaccggatca
     2221 tgcatgccgg tcccagccgg cgggttgagc gagccgggct gtcgctgtcc cgagagggag
     2281 accagaccgg tgccgggagg gtggaatgag acgcacacag acaccggaga ttggcgccgg
     2341 tgtctgtgtg cgtctcattc cccacaatga ggtatctgca gtttgaggca gtattggacg
     2401 ccgagcatcc cccgaaagag gtatttgagg gagtggacga ggaagagaca cggaacaagc
     2461 cgaatcccct tggctggaga gtgaaggcat cgccgtgctg tcatggctgc gtacgcggag
     2521 atccccgggc tgcatgaggg agcgttgtac atggagacat gcttgatggg gctcgtgcta
     2581 gagacctttc ccgccgatga cctgcaactc gaactgatcg gacgtggacc ggctcatcag
     2641 cggtatcgct tgcccgatca gtgctcgaac ctcggccgat tcgagcgacg cctcatgcgc
     2701 atccttcgtc gtccaggtct cggtcaccca gatcgcatca gcctcgtccg ttgtgctgat
     2761 gaggtagtgg atgcagtcgt cattgcg
//
LOCUS       DASYWM010000290         6063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_445936, whole genome shotgun sequence.
ACCESSION   DASYWM010000290 DASYWM010000000
VERSION     DASYWM010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445936"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VGR29_11745"
     CDS             complement(<1..501)
                     /locus_tag="VGR29_11745"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074301.1"
                     /translation="MATETERFQFDPAVSSAVPAGSDVRQLQQWEGPLDAEELDRMLR
                     YWSASNYLTIGQIYLRENPLLREPLKPEHIKPRLLGHWGTSPGLNMIYVQMNRLIKRH
                     HFDAIYIAGPGHGGPSLVAHTYLEGTYSEIYHTVSADEPGMRQLFRQFSTPGGVPSHV
                     SVPTPGS"
     gene            complement(960..1367)
                     /locus_tag="VGR29_11750"
     CDS             complement(960..1367)
                     /locus_tag="VGR29_11750"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2074302.1"
                     /translation="MVENQLGDNDTIYGTFNLAEVMSEFPPDPGSAARHRAEILVKTD
                     TLRVVLVTMLSGGFMHEHAAPGPITIQVLKGVMNLTVEGESRTLSAGELISLAPGIMH
                     GVEGVEDGAFLLTIAHLSRIPDPGGDEDVPQGL"
     gene            complement(1590..2381)
                     /locus_tag="VGR29_11755"
     CDS             complement(1590..2381)
                     /locus_tag="VGR29_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2074303.1"
                     /translation="MSNHEIKVGVQIQPQHGTFEQNRQAWIEADELGVDTIFNWDHFY
                     PLTGDANGNHFEAYTLLGSMAEVTERAQIGSLVSCNSYRNPHLLADMARTLDHISGGR
                     FILGIGSGWFERDYLNYEYEFGTAAGRLQDLKRDLPKIVERLGKLVPGPVNGHMPMLI
                     GGSGPKVTLRLVAEYADIWHAFGTPEEMAKKGEILDGHCADVGRDPNEIERSSGINVK
                     TLDQADAYVEKGFTHLIMGVTGPEYDLGPARELVQWRDQARKSQG"
     gene            complement(2431..3918)
                     /locus_tag="VGR29_11760"
     CDS             complement(2431..3918)
                     /locus_tag="VGR29_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2074304.1"
                     /translation="MASGSTVQDRTREPESRGKFAPGLTPVSLFLVLVLFWVLTKTGL
                     VLVLGLLALLLGTVLEGPVRRLEDRHFPRAAAIATVYAVLIGSVVALVLLIMPAVRSQ
                     ADDFQEQIPVQLRELEQDWENSSNPLLSGPGRDFLRTGIEFFEEPTSADVAVPDDAAQ
                     RAIPIVTTVGGALVSTLTLLVITFYYLLEKKLIRSLVIEQLSPKFQPRVDRLWTEVEN
                     KVGGWMRGQLMLCLIIGTIATISYGIIGLSFWPLLGLWAGITEIIPIVGPWLGGIPAV
                     IVALTQGWQTALIVGLIILGMQSLENWFLVPRVMRGAVGLTPLAVFLAILAGTQLMGI
                     PGAVLAIPIAATIQVILTDWIDQRKIRRTSEVEPVSGWRWMLNRGVGRENPPDASDGA
                     RGSGAPTGRGQSVSDQDVLGRENVGEGPQGDAGPDPDRESDETAVATWPANPWKNRGS
                     EASAGPAWRGMSNSKRPGPSPASPAGKPAQRDDTSIEAVDKDDIE"
     gene            complement(4076..4456)
                     /locus_tag="VGR29_11765"
     CDS             complement(4076..4456)
                     /locus_tag="VGR29_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074305.1"
                     /translation="MNNSNPFAPQRERLRTRTPMRCPICHSTLGKVIVRDIGSAAPGA
                     IWQIHAGECADHGWFQTEVVRRPPREIFPVTKPFGAARRVVVAGREYYAFSTAWNELP
                     GEERRQPTDPLEERYWQALPLPGV"
     gene            4707..5171
                     /locus_tag="VGR29_11770"
     CDS             4707..5171
                     /locus_tag="VGR29_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074306.1"
                     /translation="MDTGIQSTQRGSGHAALIDQLVDTYRELNTHVRRLPEERLTAGG
                     EEGSVRSVIARMRIDEMRFAQALKERITGVIHADLADKDPVIGTEHRNDTTVMLISQF
                     GTARETTLNLLKNLTDEDWTKQTDDGKSIQDHVQDLVASDRNQLERIKQLLT"
     gene            5185..5784
                     /locus_tag="VGR29_11775"
     CDS             5185..5784
                     /locus_tag="VGR29_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074307.1"
                     /translation="MDSSTEPVMQPEWLQESPPQRPSYDPRDVLYRTQRFSLVRAHIS
                     AFAFGSVLLLVVNLLIGSGSIWAATLIGAWALIVVMHAIIAGIASLILQLLAEDDDIR
                     PASEVRWRPAKTWAATEPPAEPASSWSSPPAPETAPTADPLPDQWTAPPPPPKDTERV
                     SWQTAAQAAWLARPVPTRAEDPSRQPDGGTKPPEPDSNG"
BASE COUNT         1201 a         1939 c         1795 g         1128 t
ORIGIN      
        1 cgagccgggt gtcggcaccg aaacgtggct cggcacgccg ccgggcgtcg agaactggcg
       61 gaagagctgg cgcatgcccg gctcgtcagc ggagacggtg tggtagatct cggagtaggt
      121 gccctcgaga taggtgtggg caacgagtga ggggcctccg tgaccaggac cggcgatgta
      181 gatggcatcg aagtgatgcc gcttgatgag ccggttcatc tgcacgtaga tcatgttaag
      241 gccgggcgag gtcccccagt gaccgagcag gcgcggtttg atgtgctcgg gcttcagcgg
      301 ctcgcgcagc agcggatttt cccgaaggta gatctggccg atggtgaggt agttcgacgc
      361 cgaccagtag cgaagcatcc ggtccagctc ctcggcgtca agcggccctt cccactgctg
      421 gagctgtcgg acatcgctcc ctgcggggac ggcactggag acggctgggt caaactggaa
      481 tcgctctgtc tctgtcgcca tggtgtctgc cctgttccat tgataagcgt gcgggaggta
      541 ctcgttttag tagaagcgcc ccgcgagtgt cgaaactgcg gccgcgagat gccgccgaga
      601 tcaatcggat tcccgcgggc ctcgtcgact cccttccatt attgtcattg atcccggcag
      661 ggaatgccgt tgcagaaccc tgtcaaaaac tgaagaggag atgggaaatt cgtgtacgac
      721 caataacgac ccggcggtca agccgaccaa gatcgctgca agacacatac gatgttggac
      781 gtcagcctct acggcacagc atggacgtga cccgcaggcg cgttccgttc cgatccattg
      841 accagagcgg gacaacatca agaaaatcca ccccgcccga gcgacgggcg gtaagttggc
      901 tgtccaggaa acgaaaacgc ccgatcatgc tgtctgaccg ggcgttttcg tcaattggcc
      961 tagagtccct ggggcacatc ctcgtcgccg cccggatcgg gaatccggct gagatgggca
     1021 atagtgagga ggaacgcgcc gtcttcgaca ccctcgacgc catgcatgat tcccggtgcg
     1081 agcgagataa gctcgcccgc ggagagtgtc cgcgactccc cctcgaccgt gaggttcatc
     1141 acacccttga ggacctggat cgtgatcggt ccgggcgcgg catgttcatg catgaagccg
     1201 ccgctgagca tggtgaccaa cacaacccgc agtgtgtcgg tcttcaccag gatctccgcc
     1261 cggtggcgag ccgcgctccc aggatccggc ggaaactcgg acatcacctc agcgagattg
     1321 aatgtcccgt agatcgtgtc gttgtcgccg agttggttct cgaccatctg cggttcctcc
     1381 tgctctcatc gggcatcccg gggagcacct cccctaccct cgtcgcgttg tttccgcgag
     1441 ccctgtcgca agtatcctgc cgaacagcga cgatgtggcg gtcagcctgc gatccaggaa
     1501 catcctctca ttgcgacgcc ctagacgatc tcgagccgag cccctgcgag aagcagggac
     1561 cgcgcagcgc ccctgggcgc aacccgcggt tatccctggc tcttgcgcgc ctggtcccgc
     1621 cactggacga gttcccgtgc cggtccgagg tcgtactccg gcccggtcac gcccatgatc
     1681 aggtgggtga agcccttctc cacatacgca tcggcctggt ccagcgtctt cacattgatg
     1741 ccggacgagc gctcgatctc gttgggatcc cttcccacat cggcgcaatg cccgtcgaga
     1801 atctcaccct tcttcgccat ctcctccggc gttccaaacg cgtgccagat atccgcatat
     1861 tcggccacga ggcgcagcgt taccttgggc ccgctaccgc cgatcagcat cggcatatga
     1921 ccattgaccg ggccgggaac cagcttgccg aggcgctcga cgatcttcgg caggtcccgc
     1981 ttgaggtcct gcagccggcc ggcagcggtg ccaaactcgt actcgtagtt gaggtagtcc
     2041 cgctcgaacc agccggagcc gatgccgagg ataaaccggc cgcccgagat gtgatccagc
     2101 gtgcgcgcca tatcggccaa cagatgggga ttgcggtagg aattacacga cacgagcgag
     2161 ccgatctggg cgcgctccgt cacctcggcc attgagccaa gcagtgtata agcctcgaaa
     2221 tggttgccat tggcatcgcc agtcagtgga tagaaatggt cccaattaaa aatggtgtcg
     2281 acgcccagct catcggcttc gatccatgcc tgccggttct gctcgaacgt gccgtgctgc
     2341 ggctggatct gtacgccgac cttgatctcg tgattgctca tgcgtggtaa ttgtctcccc
     2401 gtcactgtta cagacatccc ctaaaggcgt ctattcaata tcgtccttgt cgaccgcctc
     2461 aatggaagta tcgtcgcgct gtgcgggctt ccctgccggg cttgccggcg atgggccggg
     2521 ccgcttcgag ttcgacattc cccgccatgc cggaccggcg ctggcctcgc ttccccggtt
     2581 tttccagggg ttggccggcc aggtcgctac cgcggtctcg tctgattccc ggtccggatc
     2641 cggtcccgca tcgccctgag gcccttctcc aacgttctct ctccccaaga catcctggtc
     2701 gctcacactc tgcccgcgac cggttggcgc accgctgcca cgagccccat cgcttgcgtc
     2761 cggtgggttc tcacggccaa caccgcgatt gagcatccat cgccacccgg aaacaggttc
     2821 cacttccgag gttcggcgaa tctttcgctg atcgatccaa tcggtcaaga tgacctggat
     2881 ggtcgccgca atggggatcg caaggaccgc gccggggatg cccattaatt gtgttcctgc
     2941 caaaatcgcc aggaacacgg ccagcggcgt cagccccacc gcaccgcgca tcacgcgcgg
     3001 taccaggaac cagttctcca gcgactgcat gccaagaatg atgagcccga caatgagagc
     3061 ggtctgccat ccctgcgtca gtgccacgat caccgccggg atacctccca gccaggggcc
     3121 cacgatcggg atgatctccg tgatgcccgc ccagaggcca agcagcggcc agaaactgag
     3181 cccgataatc ccataggaga ttgtagcgat cgtgccgatg atgagacaga gcatcaactg
     3241 cccccgcatc cagccaccga ccttgttttc cacctcggtc cagaggcgat cgacacgggg
     3301 ttggaatttc gggctgagct gctcaattac gaggctgcgg atcagtttct tctcgagcag
     3361 gtaatagaag gtgataacca gcaaggtcag ggttgacacc agcgcgccac caactgtcgt
     3421 tacgatcgga atcgcccgct gggcggcatc gtccggcacg gcaacgtccg ccgatgtggg
     3481 ttcctcaaag aattcgatac cggtgcgcag gaagtcacgc cctggtccac tgaggagagg
     3541 attgctactg ttttcccaat cctgctccag ttcccgtagc tggactggaa tctgctcctg
     3601 aaaatcatca gcctgggacc gaactgccgg catgatcagg aggacgagcg caactaccga
     3661 gccgatcagt acggcgtaga ccgttgcgat cgcggcagcg cgggggaagt gacgatcctc
     3721 caggcgccgt acgggcccct caagaactgt cccgagcagg agggccagca gaccgaggac
     3781 gagcaccagc ccggtcttgg tgaggaccca aaagagcacc aggaccagga agagcgagac
     3841 cggtgtgagc ccaggcgcga acttgccccg ggattccggc tcacgcgtcc gatcctgtac
     3901 agttgaacct gatgccatac acatagtctc cagctcgtca gcgacgaaaa attgcaggcg
     3961 gtctccctgc tggattccac aataaacccg cctgggcggc tccccttgtc gtgatgggac
     4021 caagggaccg ccaaagcggg tcaggactcg cagccgtgcc gctcagcggc cggcgtcaaa
     4081 cgccaggaag tggcagcgct tgccagtacc tctcctcaag cgggtcggtt ggctggcgcc
     4141 gctcctcacc cggaagctca ttccatgcgg ttgaaaaggc gtagtactcc cgaccagcga
     4201 ccacgacgcg ccgagcggca ccaaaaggct tggtaacagg gaagatttcg cgtggcggac
     4261 gccgcacgac ctcggtctgg aaccatccat ggtctgcgca ttcgccagca tgaatttgcc
     4321 agatcgcacc aggagcggcg ctgccgatgt cgcgaacaat gaccttgccc aacgtgctgt
     4381 gacagattgg acaacgcatg ggggtacgtg ttcgaagacg ctcgcgttgg ggggcaaaag
     4441 ggttggaatt gttcatgctg aacatctttc gtcttccaat gtcctgacgc gccgacagac
     4501 caagcccgta gtgatgctga caaaagggag tgactgcctg cttctcccga tagtttacca
     4561 tccagcccgg gaactaccca tcgcgatctg caccctgtgc gcccccacta gcgggttcgg
     4621 ctgtaatccg cgaaactact ggtatacggt gtgcgaagcc gaccggatga gcgacatcaa
     4681 gcatgtatcg gaaaggaacc ttacctatgg ataccggcat tcagtcgaca caacgcggca
     4741 gcggtcacgc cgccctgatc gatcaactcg tggacaccta tcgtgagctc aacacccatg
     4801 tccgtcggct gccggaagag cggctgaccg ctggtgggga agagggatcc gttcgctcgg
     4861 tcatcgcgcg gatgaggatc gacgagatgc ggttcgcaca ggcgctgaag gaacgcatca
     4921 cgggtgtgat ccatgctgac ctggctgaca aggatccggt catcggcaca gagcaccgca
     4981 acgatacgac ggtcatgctg atctcccagt tcggcaccgc gcgtgagaca acactcaacc
     5041 tcctgaagaa cctgaccgat gaggactgga cgaagcagac cgatgatggc aagagcatcc
     5101 aggaccatgt ccaggacctg gtggccagcg accggaacca gcttgagcgc atcaagcagt
     5161 tgctgaccta gttcccggat caccatggac tcgtctacag aaccggtgat gcaaccggaa
     5221 tggcttcaag agtctccgcc gcaacggccg tcgtacgatc ccagggacgt gctttaccga
     5281 acccagcgat tctccctggt gcgcgcgcac atcagcgcgt tcgcgttcgg gagcgtactg
     5341 ctgctcgtgg tcaatctcct gatcggatcg ggcagcatct gggccgcgac gttgatcggc
     5401 gcatgggcac tcatcgttgt catgcatgcg atcattgctg gtatcgcgag tctcattctc
     5461 cagctcctcg ccgaagacga cgacattcgt cccgcatcgg aggttcgctg gaggccggca
     5521 aagacgtggg ctgcgactga gccaccagcc gagccggcga gttcctggag ttcaccgccc
     5581 gctccagaga ccgcaccgac cgcggaccct ctccccgatc aatggacggc gccccctccc
     5641 ccaccaaagg acacggagcg tgtttcatgg caaaccgccg ctcaggctgc ctggctggct
     5701 cgacccgtgc cgactcgcgc ggaggatcct tccaggcagc ccgatggcgg tacaaaaccg
     5761 cccgagccag attcgaacgg ctaaaccagt ggtgattcac ccgatccacc ggcctggcaa
     5821 taccgggcag cggatcacct tctatccaat ccacgagcgg tcccaaagca catgacctcc
     5881 cctgcagtcc ccttggccaa catatgacag cactttcccg cgacttcacg gtcgccattt
     5941 ttgtgattca tgacgactgc gtgctcctgc acaggcaccg caagcttggc ctctggcttc
     6001 cgcccggtgg tcacattgag cagaacgagc ttcccgacga ggctgctctg cgcgaggtgg
     6061 agg
//
LOCUS       DASYWM010000291         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_465073, whole genome shotgun sequence.
ACCESSION   DASYWM010000291 DASYWM010000000
VERSION     DASYWM010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465073"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VGR29_11780"
     CDS             complement(<1..426)
                     /locus_tag="VGR29_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEV2074308.1"
                     /translation="MTREQVLNALSKVSEPATGRGLVDLKLVTDVAVEGRAVRLNIDL
                     LSPINTHREKLERDVRAALGTVGGLGEVDLHFRTKVRASSGGRTDAQPIPGVKNTIAV
                     ASGKGGVGKSTVAVNLAVALAQAGAKVGLLDADVYGPSTP"
     gene            624..>1521
                     /locus_tag="VGR29_11785"
     CDS             624..>1521
                     /locus_tag="VGR29_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074309.1"
                     /translation="MMSRRLSRWSIVVFAMLLIGPANLGAMISTPVADDTQLYLDPAA
                     RLTARIPADWTSVASPYMTYAGGDGLITSFLATASTLDEACEEAGDELASGSSFTSSE
                     ATWHNEPACEIDIPHPNPLVGGVRLSAMVVPHPAPFEFWLETISFVVIALDPGHFHDV
                     LSTVSFDLSALTGPEIAHAILDYAEANSFYTDDIDWDSLRAEANALASPGSISIFVNW
                     HLIPALRAAGDNQSFLLPVLANGPADESPVDRFPSGQPRGNLGYLNLPSGALPRDEQP
                     YIEAGLDAITGLDAQNVCGWIID"
BASE COUNT          272 a          501 c          425 g          323 t
ORIGIN      
        1 tggcgtgctt ggaccgtaaa catcagcatc cagcaagccg accttcgcac ctgcctgtgc
       61 aagtgccacc gcgaggttga cggcgacggt cgacttgcca accccgccct tgccggaagc
      121 aacggcaatc gtgttcttca caccggggat cggctgcgca tcggtacgtc ctccactact
      181 cgcccggacc ttcgtccgga agtggaggtc gacctcgccg aggccaccca cggtgccgag
      241 cgctgcccgg acgtcccgct ccaacttctc ccgatgggta ttgatcgggc tgagaagatc
      301 gatattaagc cggacagccc ggccctcgac cgcgacatcc gttaccagtt tgagatctac
      361 caggcctcgc ccggttgcgg gctccgatac tttcgatagt gcgttgagca cttgttcccg
      421 cgtcattgct gcggagccac catccgtcat cgacgtgcct gaacctttcc tgagactccg
      481 gagcacgccg cttctcgacg tgtactccgg gagagtactt actccctaat tgtaccggcc
      541 gcgtctaatc ctgactgctg tggtaaggat tagcgtatgt tagggtgttg gggtcagata
      601 ccgcgttagg agaggatccg cccatgatga gtcgccggct ttcccgatgg tcaatcgttg
      661 tgtttgccat gctcctgatc ggtccagcga acctgggtgc gatgatttcc acgccggtcg
      721 ccgacgacac gcaactctat ctcgaccccg cggcgcgact gacggcacgc attccggctg
      781 actggacgag tgtggccagc ccctatatga cctatgccgg aggtgacggc ctgatcacca
      841 gcttcctcgc cacggcgtcc acccttgatg aggcatgcga ggaggctggc gatgaactcg
      901 catccggttc gtcgttcacc agcagcgagg ctacatggca caacgaacct gcctgcgaga
      961 tcgatatccc gcatcccaat ccgttggtgg gcggtgtccg cctctccgcg atggttgttc
     1021 cacacccagc gccgttcgag ttttggctcg agacgatctc cttcgtggtg attgccctcg
     1081 atcccggtca cttccacgat gtcctgtcga cggtgtcgtt cgacctctcg gcgctgaccg
     1141 gtccggagat cgcccacgcg atcctcgatt acgcagaggc aaactccttc tacacggacg
     1201 acattgactg ggattccctc cgggccgaag ccaacgcgtt ggcttcgcca ggaagtattt
     1261 ccatcttcgt caattggcat ctgatcccag cactgagagc cgctggcgat aaccagtcct
     1321 ttctcttgcc cgtcctcgcc aacggaccgg cagatgagag ccctgtggat cgattcccct
     1381 caggccaacc ccgcggcaac cttggctatc tcaacctgcc gtctggcgcg ctgccacgcg
     1441 atgagcagcc atacattgag gcaggactcg atgccatcac aggcctggac gcacagaacg
     1501 tgtgtggctg gatcattgac c
//
LOCUS       DASYWM010000292         7514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_145699, whole genome shotgun sequence.
ACCESSION   DASYWM010000292 DASYWM010000000
VERSION     DASYWM010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145699"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VGR29_11790"
     CDS             complement(<1..432)
                     /locus_tag="VGR29_11790"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2074310.1"
                     /translation="MEDRVLSQTDIQQRADLIRPGHAQDSAAQSTISVARLLQELRES
                     RGLSKADLAKGAGVDPSSITRFEQGTRTPERETILQMSRAMMLPMLDRDRLLAAGGFR
                     SEVWDDPLLVELAQLLVEPSVPAEARDEARAAVKMAVAYCKL"
     gene            730..2766
                     /locus_tag="VGR29_11795"
     CDS             730..2766
                     /locus_tag="VGR29_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV2074311.1"
                     /translation="MATPTLTTETTIRPSSNSRGRAQVVFDWVRSWRLAILAALSWAF
                     TIGGLVADYASDISTPTLVGIFMLAYLGGGTLATGAAISDLLDRKINVDLLMVLAAVG
                     AAALGSWTEGAILLSLFSTSNALEHHALDKTRNAVRLLMDLTPPTARVERDRALRIIP
                     VEELRLDDVIIVQPGEKIPADARVLSGSSTVDQAAITGESIPAGKIPGDTVFAGTING
                     HGALQARVTRLSTESTLARIVKLVEEAREQKSKLEQFADSFEGRYAAAVIVFSLLVAT
                     VPILFGVDPGDAFYRGMTLLVVMSPCALVISTPAATLSALANAARRGVLVKGGRSLET
                     LGTVDTIAFDKTGTLTVGRPRLTDVVMLNGADPGDILARAAAAEQLSEHPIGTAIVEA
                     ARYQELILPPSSDLEAHVGRGITARVNGEVLAIGNERLFRDLGIDLPKAAVEHQNRLG
                     EEGKSTMIVGDRAGVYAVLAVADTLRPGVRETVAALRKAGVKRTVMLTGDNALVANAI
                     ARSVGIDEVHADLLPEEKLAVIETLKASGKVAMIGDGVNDAPALAMADVGISMGGAGS
                     DVALETSDVVLMSDDLGKLYGTISLSRAMRKTIRANLTFALSIITVLAISTLIVGIPL
                     PLGVVGHEGSTVVVVLNGLRLLAHGGNFTLRARPQGSDQRRDHHEAPQYRLTIA"
     gene            complement(2897..3127)
                     /locus_tag="VGR29_11800"
     CDS             complement(2897..3127)
                     /locus_tag="VGR29_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074312.1"
                     /translation="MKRRAATVVLALAFSLSLVHGAMAAPTVSDNASHNACFGQARAD
                     YAIHRDGPEVTIPERGGENPAENAAWTAEHCS"
     gene            complement(3497..4156)
                     /locus_tag="VGR29_11805"
     CDS             complement(3497..4156)
                     /locus_tag="VGR29_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2074313.1"
                     /translation="MRIVIAEDSVLLREGLIRLLTEHDAEVVAAVADSDALIEAVVRE
                     RPDVSIVDVRMPPTFRDEGLRAAIELRRRIPNTPILVFSQYVEERYAAELLERGTSGI
                     GYLLKDRVADVRDFIHGVRQVAAGGTVLDPEVVAQLVTRRRQDERLASLTPRELEVLA
                     LMAEGRTNQAIADQLFVSEGTVEKHIRGIFMKLQLEDAETMHRRVLAVLTYLRLGEDE
                     R"
     gene            complement(4147..5514)
                     /locus_tag="VGR29_11810"
     CDS             complement(4147..5514)
                     /locus_tag="VGR29_11810"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV2074314.1"
                     /translation="METTQAGAPPRMPDGHQDRDRMKAPFRVSNQRVQRVLDDPNSLW
                     FLLRNLPGVRSWRILSDSLFLLTSFPLGLVWFIVGVIGLTLGFALSFIGVGLVILAVT
                     IGLLIWAAQVERTRLRVFLGVNVAPTIPISHYEGNIARRTWRYIRNPQMWRDFIYLMV
                     LFPIGIAELTLVFVPINLLFGPFMYGLFGSYHVAFWHVNHGFEAWFAVIVGMVLILPL
                     SILINVAAAFHGRLGVMLLGTSKEEVLYERVEALTESRSAVMRAMHMERRRIERDLHD
                     GAQQRLVNLAMDLGRAREKMDTDPEGARQIVEQSHEQAKQVLTEMRDLVRGIHPAVLT
                     DRGLDAAVSAIAGRSPVPVKVDVQIDGRLLEEVEGTAYFVVVEALTNIARHSDATQAS
                     VQIRRDGNWLRITVTDDGRGGADPEMGSGLRGLKDRITALDGSFALSSPAGRGTRLIV
                     EIPCA"
     gene            complement(5747..6988)
                     /locus_tag="VGR29_11815"
     CDS             complement(5747..6988)
                     /locus_tag="VGR29_11815"
                     /inference="COORDINATES: protein motif:HMM:NF006775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEV2074315.1"
                     /translation="MSQEQLAKEIMARADELAQYSEKPDGICRRYGTSEMRAVLERTR
                     EWMLAVDLDVTTDAFGSLIGIYAAEEGAAEHRPFVIGGHLDSVRDAGRYDGILGVLTG
                     LAVIEQMHRQNRRLPFPVQLIAFAEEEGLRFNSTLVGSRAWTGLPVAEQLAYTDEAGI
                     SLREAIRAFGGDPDSLGSGEHPDLLGFLETHIEQGPVLEHEDLPVGVVTSIVGSDRAE
                     IKIAGMAGHAGTVPMALRRDALTAAAELVLEVERIGRQTPGLVATVGELEVHPGATNV
                     IPGSVRLTCEVRHASGEVCTDAIRAIRSSLETICEKRGTSFGWRDIAGYPATPCDPGL
                     VHLLSEAVRAEGVRVLTLASGAGHDAVNIGQIAPVSMLFMRCKEGISHNPAESIHVAD
                     VEVAIRVMLGFLDLIASRQAG"
     gene            7182..>7514
                     /locus_tag="VGR29_11820"
     CDS             7182..>7514
                     /locus_tag="VGR29_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074316.1"
                     /translation="MRKRAPVIDTDIHPTMDIRRVAEFLAEPWHSRFASGNRGPGTLG
                     YWNSAGFHQADAVTEDGEGIASHPETLSRLFFDVYGLEYGVLNSETAIHIGVGPEMDF
                     GAAVLSAMN"
BASE COUNT         1476 a         2456 c         2174 g         1408 t
ORIGIN      
        1 cagcttgcaa taggcaacgg ccatcttcac agcggcacgc gcttcgtctc gtgcttctgc
       61 cggtaccgaa ggctcgacca gcaactgggc cagttccacc agcaggggat cgtcccacac
      121 ttccgaccgg aacccaccag cagcgagcag gcggtcgcga tccagcatcg gcagcatcat
      181 ggccctggac atctgcagga tcgtctcccg ctccggtgtc cgggtgcctt gctcgaacct
      241 ggtaatggag ctggggtcga ccccggcacc cttggccaga tccgctttcg aaagcccgcg
      301 gctctcgcgg agctcctgca gcagcctggc gacggagatc gtcgactggg cagcggagtc
      361 ctgcgcatgc cctggcctga tgagatcagc ccgctgctga atatccgtct ggctcagtac
      421 ccgatcttcc acctgccact cctccacctc tcattgtgag catttttatg ggtagcatac
      481 ccgttttgct gaccgcctat ccctacagct gccccggatg aatggcgtct ggacgaccct
      541 cctcatgttc ctgcgaatca ctctcaaaaa gggtttcaaa gagcaccgga caatgatatg
      601 atgagtcagg aaatgagaat gattctcaac tacgacctga acgctcggaa ttgaatgccc
      661 gctgcagaga cagaaggcag cggcccacgg accactattt cgccaactga ctccatttaa
      721 ggattgcctg tggcgactcc gaccttgaca actgaaacga ccatccggcc gtccagcaac
      781 tccaggggaa gggcacaggt tgtgttcgat tgggttcgct cctggcgtct tgcgatcctg
      841 gcagcgctat cttgggcctt caccattggc ggtctggttg ccgactatgc ttccgacatc
      901 tccacgccaa cgctggtcgg catctttatg ttggcgtacc tggggggcgg cacccttgca
      961 acgggtgccg caatctccga tctcctcgat cgcaagatca acgttgacct cttgatggtc
     1021 cttgccgcgg ttggcgccgc agcgctcggc tcctggaccg agggcgctat cctgctctcc
     1081 ctcttctcga cctccaacgc acttgagcac cacgcgctgg acaagacccg gaacgccgtt
     1141 cgcctcttga tggacctcac gccgccgact gcacgggtag aacgcgaccg cgcgctgagg
     1201 atcatcccgg ttgaggagct caggctggac gatgtcatca tcgtgcagcc tggggagaag
     1261 atccccgccg acgcacgagt cctctctggg tcatcgaccg tcgatcaggc ggcgattacc
     1321 ggcgagtcga ttcccgccgg gaagatcccg ggtgacaccg ttttcgccgg gacaatcaac
     1381 ggtcacgggg cattgcaggc ccgggtaacg aggctcagca ccgaatccac actcgcccgg
     1441 atcgtgaagc ttgtcgagga agcgcgcgag cagaagagca agctggagca gttcgccgat
     1501 tccttcgagg gccgctatgc cgccgcggtc atcgtctttt cgctacttgt agcgacggtg
     1561 ccgattctct tcggggtcga ccccggcgat gcgttttacc ggggtatgac gctccttgtg
     1621 gtcatgtcgc catgcgcgct ggtcatctcg acaccggcgg cgaccctctc cgcgctggcc
     1681 aatgccgccc ggaggggcgt tctggtcaag ggcggccgat cgctggagac ccttggcaca
     1741 gtcgacacca tcgccttcga caagactggc acgctcaccg tgggacgccc ccgtctgacc
     1801 gatgtcgtga tgctcaatgg cgccgatccc ggtgacatcc ttgctcgcgc cgctgccgcg
     1861 gagcagctct cggagcaccc catcggcacc gcgatcgttg aggctgcgcg gtaccaggaa
     1921 ctgatcctcc ccccatcttc agacctcgag gcccatgtcg gacgcggcat aaccgctcgg
     1981 gttaatggtg aggtcctggc catcggtaac gagcgcctct tcagggacct cggaatcgac
     2041 cttccgaagg cggcggtcga gcatcagaac cgccttgggg aagagggtaa atcgacaatg
     2101 atcgtcggcg accgggccgg cgtttatgcc gtcctcgccg tcgccgacac cctccggcca
     2161 ggggtgaggg agacagttgc cgcgttgagg aaggctgggg taaagcgcac cgtgatgctg
     2221 accggcgaca acgcgctggt tgcgaatgcg attgccagga gcgtcggcat cgacgaggtc
     2281 catgccgatc tcctgccgga ggagaagctg gcggtgatcg agacgctgaa ggcaagcggc
     2341 aaggtcgcca tgatcggaga tggtgtcaac gacgccccgg ctctcgcgat ggccgatgta
     2401 ggcatctcca tgggcggcgc gggctccgat gtggctctgg agacttcgga tgtcgtcctg
     2461 atgtcggacg acctgggaaa attgtacgga accatcagcc tcagccgcgc gatgcgaaag
     2521 actatcaggg ccaatctcac ctttgcgctt tccatcatca cggtgctcgc gatctcgacg
     2581 ctgatcgtcg gcatcccgct tccgctcgga gttgtcggtc acgagggcag caccgtcgtc
     2641 gtcgtgctga acggcttgcg gctactggcg cacggcggaa acttcaccct ccgcgcccgg
     2701 ccacaaggct cagaccagcg acgtgatcat cacgaagcgc cacagtaccg gctcacgatc
     2761 gcgtaattcg ccttgatgac ccgcgcttta ctaggctcca ccttgttcga ccatttgagc
     2821 ccatgaatac gacatgccag aaggggaagc gctggcacca gcgcttcccc ttctggcaat
     2881 gaatatcaaa cctatattac gaacaatgtt ctgctgtcca tgcagcattt tcagccgggt
     2941 tctcaccccc acgctcgggg atcgttacct ccggcccgtc acggtgtatc gcgtagtcgg
     3001 cgcgcgcttg tccaaagcac gcattgtggc tcgcgttgtc actgacggtt ggcgccgcca
     3061 tggcgccgtg aaccaaggag agcgagaatg ccagggccaa taccacggtt gctgcacgac
     3121 gcttcatcag agtccactcc ttgatccttg tgctgacgct gcggcccagg taccacatca
     3181 tcaaactgcg cttaccggga ggccgtcccc cgacttctct cgcagcatag ggctccagcc
     3241 tatggctggc tgtcgtccac ggcacagctc atgtgtgcgc ttcgacacac aaggcccgca
     3301 cacgacaacg gcccgtacgt gatgtacggg ccgttgtcgt gtgccttcgg cattcctctc
     3361 accgggtatg cttcaaccca gcagactacc tgataaccgg gttttcgaca tcctggagcg
     3421 gcgcgaaacc acacccgcca gaagggcact ggtcgcccgc ccctttttgt cgtcactcag
     3481 tcgtaacaat ggatcgttac cgttcatcct cgccgaggcg gagatacgtg agcaccgcaa
     3541 gcacccgacg atgcatggtc tcggcatcct ccagctgcag cttcataaag atcccacgaa
     3601 tgtgcttctc aactgtgccc tcgctcacga aaagctgatc ggcgatcgcc tggttggtgc
     3661 gcccctccgc catcagcgcc agcacctcga gctcgcgtgg cgtcagcgat gccagccgtt
     3721 catcctgtcg acggcgggtc accaactggg ccacaacctc ggggtcaagg acagtacctc
     3781 cagcagcgac ctgcctgacc ccgtggataa aatcgcggac gtccgcgacg cgatccttga
     3841 ggagataacc gataccgctg gttccccgtt ccagcaactc ggcggcatag cgctcctcaa
     3901 catactggga gaagaccagg ataggtgtat tgggaattcg ccggcgcagc tcgatcgcgg
     3961 cacggagccc ttcgtcgcga aaggttggag gcatgcgcac atcgacgatg gatacatccg
     4021 ggcgttccct cacaaccgcc tcgatcaggg catcgctgtc ggccacggcg gccacgactt
     4081 ccgcatcgtg ctcggtgagg agccggatca gtccctcacg caacaggaca gaatcctcgg
     4141 cgatgactat gcgcatggga tctccacgat aagccgggta ccgcgtccgg ccgggctgga
     4201 gagcgcgaag cttccatcca atgcagtaat acgatccttg agccctcgca ggccagatcc
     4261 catctcgggg tcagcaccgc cccggccatc gtccgtcacg gtgatccgga gccagttccc
     4321 gtcccggcga atctgcaccg acgcctgggt cgcatcgcta tggcgggcaa tattggtgag
     4381 cgcctccacg acgacaaagt aagccgtgcc ctccacctct tccagcagcc gaccgtcgat
     4441 ctgcacatcc actttcactg gaaccggtga tcggccagcg atagcggaga ctgccgcatc
     4501 cagcccccgg tcggtcagga cagcggggtg aatcccgcgc acgaggtcac gcatctccgt
     4561 cagtacctgc tttgcctgct cgtgcgactg ctccacgatc tggcgcgctc cttccggatc
     4621 ggtgtccatc ttctcgcgtg cccgtcccag gtccattgcc agattgacca accgctgctg
     4681 ggcgccgtca tgcaggtcgc gctcgatacg gcgccgctcc atatgcattg cccgcatcac
     4741 cgcggagcgg ctttccgtca gtgcctccac ccgctcgtag agcacctcct cctttgaggt
     4801 tcccagcagc atcaccccca ggcgaccgtg gaacgcggcc gcaacgttga tcaggatgct
     4861 caacggaagg atgagaacca taccgacgat caccgcgaac cacgcctcga acccgtgatt
     4921 cacatgccag aaagcgacat ggtaggaacc aaacagccca tacatgaacg ggccaaacag
     4981 gagattgatc ggcacgaaca ccagcgtcag ctcggcgatg ccgatcggga agagcaccat
     5041 caggtagatg aaatcccgcc acatctgcgg gtttcggata tagcgccatg ttcgccgcgc
     5101 aatgtttcct tcgtagtggg agatcggtat cgtcggcgcc acgttgaccc cgagaaagac
     5161 cctcagccgc gtacgctcga cctgcgccgc ccagatgagc aacccgatcg tcacggccag
     5221 gatcaccagg ccaacgccaa tgaaggacaa ggcaaagccc agcgtcaggc caatcacgcc
     5281 gacgatgaac cagacgagcc cgagggggaa gctggtcaga agaaagaggc tgtcgctcag
     5341 gatccgccag gaacgcactc ccggcaggtt ccggagcagg aaccagagcg agtttggatc
     5401 gtcgagcacc cgctggaccc gttgattgct gacccggaag ggtgccttca tccgatccct
     5461 gtcctgatgg ccgtccggca tacgtggtgg cgcgcccgcc tgcgttgttt ccatgatagc
     5521 gatcaccttg caactgatag aggaaagcag acagtcgtga ccttcggatc aggtcaggac
     5581 ctggcgaagc gcacgacgag caggatcacc aaacattggt aacggatcat gatgaaacct
     5641 ccctccgcaa cgatctcggc cacctctgcg gccatagctc aatcatcact ggagagggcg
     5701 gggtgccaga atgacgctga cggtggttta ggtggtaggg ctatccctac cccgcttgtc
     5761 tcgatgcgat caaatcgagg aagccgagca tcacacggat ggcgacttcg acatcggcga
     5821 catggatcga ttcggcgggg ttgtgactga tgccctcctt gcagcgcatg aagagcatcg
     5881 acaccggggc aatctgacca atatttaccg cgtcgtgccc ggcaccgctt gcgagtgtga
     5941 ggacgcgcac cccttccgcc cggaccgcct ccgacaggag gtgcaccagg cccgggtcgc
     6001 agggtgtcgc cgggtacccg gcaatgtcgc gccagccgaa tgaggtcccg cgtttctcgc
     6061 agatggtttc gagagacgag cggatggccc gtatggcgtc cgtacaaacc tcccctgacg
     6121 cgtggcgcac ctcacatgtc aggcggactg acccggggat gacattcgtc gcacccggat
     6181 ggacctccag ctcgcccacg gtggcaacca accctggtgt ctgccggcca atccgctcga
     6241 cctcgaggac gagctccgcg gctgcggtga gcgcgtcgcg acgcaacgcc atcggcaccg
     6301 ttcctgcatg tcctgccatt cccgcgatct tgatctccgc gcggtcactc cccacgatcg
     6361 acgtcaccac accaaccggc aagtcctcat gctctaggac cggcccttgc tcaatatgcg
     6421 tttccaaaaa cccgaggagg tccgggtgct cgccggaacc cagggaatca ggatcacccc
     6481 caaacgcccg gattgcttca cggagagaaa tcccagcctc atccgtgtac gccagctgtt
     6541 ccgccacagg aagcccggtc caggcgcggg agccgaccag cgttgagttg aagcgcagcc
     6601 cctcttcctc ggcgaaagca ataagctgga ccggaaatgg aagccgtctg ttttgccggt
     6661 gcatctgctc gataacggca aggccggtca agacaccaag gatgccgtcg tagcgcccgg
     6721 cgtcgcgtac ggagtcgaga tgcccaccaa tgacaaacgg gcgatgctcg gctgccccct
     6781 cctccgcggc atatatcccg atcaggctgc caaaggcatc tgtcgtcacg tccaaatcaa
     6841 cagcaagcat ccattcgcga gtgcgctcga gcaccgcgcg catctcggag gttccatacc
     6901 ggcgacagat gccatcgggc ttctcggaat actgcgccag ctcatcggcg cgggccatga
     6961 tctcttttgc caattgttcc tgggacatgg tggaaagcat ctccaacgtt tgcattcaag
     7021 aggcaacact actccaggca gcatcgccgg ttggatggct tgcggcaagc cggtgtcacc
     7081 atcccgtgat ggttctgctt cctgttgaca agcccccatc gtttggtcag cataccgcca
     7141 ttgaaagtat tcaagcttca ccgagacagg gaatcaggga catgcggaag agagctcccg
     7201 ttatcgacac agacatccat cccacgatgg atattcggcg cgttgcagag ttcctcgctg
     7261 aaccctggca tagccgcttt gcgtcaggca accgtggtcc cggcacgctt ggatactgga
     7321 actccgcggg tttccaccag gcggacgcag tcaccgagga cggcgaaggc attgcgtcgc
     7381 acccggagac gctctcccgg ttgttcttcg atgtatacgg gcttgaatac ggcgtattga
     7441 actccgagac cgccatccac atcggtgttg ggccggaaat ggatttcggc gccgcagtac
     7501 tctccgcgat gaac
//
LOCUS       DASYWM010000293         9346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_370920, whole genome shotgun sequence.
ACCESSION   DASYWM010000293 DASYWM010000000
VERSION     DASYWM010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370920"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VGR29_11825"
     CDS             complement(<1..527)
                     /locus_tag="VGR29_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2074317.1"
                     /translation="MFRLDGKTALVTGGASGIGREIAVLFGQQGATVAIGDINEGAAA
                     SVAEEITDAGDAAFGVRLDVTNMVSAESAIASIVDRTGSLDILVNNAGIGMVGNLTET
                     SPEDFARLIAVNVYGVYNCSHAGVKQMLSQDPKGGCIINMASIAGQVAVARRFAYGTT
                     KGAVISMTQSTAMDYV"
     gene            complement(674..1276)
                     /locus_tag="VGR29_11830"
     CDS             complement(674..1276)
                     /locus_tag="VGR29_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074318.1"
                     /translation="MNEIERPWSAMPPRAVASLFRVYPGRWWIAGGWSLDLYLGHPTR
                     LHEDTDVLVLQRDLEHVHALLPGWVIMAAAPPGTLRHWIEGETLPEGTRDIWCRPPSS
                     DVWRFQLMIIRTEGDRWIFPRDSRITGEIAQLSDVRDGIPIIAPELQLLYKSRVPHCS
                     RDADDLRRMIPRLSQRQQAWLRERVELLYPDSPALELLQL"
     gene            complement(1282..1668)
                     /gene="rbsD"
                     /locus_tag="VGR29_11835"
     CDS             complement(1282..1668)
                     /gene="rbsD"
                     /locus_tag="VGR29_11835"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEV2074319.1"
                     /translation="MKRGGILNPAINHLLASTGHTDYFTICDRGFPIPASQDRIDLAL
                     ADDLPTVLDVIRLVDTEFVIDRIILAEEAESFSPGFVDELRAIRPDLRIETVPHLELK
                     RLSSRGRATIRTGDTTPFANILVISG"
     gene            complement(1665..1892)
                     /locus_tag="VGR29_11840"
     CDS             complement(1665..1892)
                     /locus_tag="VGR29_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074320.1"
                     /translation="MSSDHDRWEYHVEHSDEPLTQQHLNELGAEGWELVAALPTAGHY
                     LFKRTAPDLRERITLEQRDALHSGQETGPVE"
     gene            complement(1889..2638)
                     /locus_tag="VGR29_11845"
     CDS             complement(1889..2638)
                     /locus_tag="VGR29_11845"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV2074321.1"
                     /translation="MSQHARKPIPEPASQIERRNLGTDVYRVLRDRILRRELRAGEKL
                     SDLRLSSELGVSRTPIREALLQLVQDGVVVAEPNRGFFVATFSRKDLEEIFDLRAALE
                     TFALRQITRSDHQEEYERALFELEHAERLIRFATTEDEREAAAEAFLKTDRGFHSWLV
                     EKSGNRRLISIVTGLWAQISIFQQAGAELPGWMELALHQHRTIIQRLVDDDIDGAHSA
                     LESHLLDMKARVLADLAPFDDGVQDESGEAT"
     gene            2811..3986
                     /locus_tag="VGR29_11850"
     CDS             2811..3986
                     /locus_tag="VGR29_11850"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV2074322.1"
                     /translation="MMVSLASRMERLGTETAFQVMARARALEAQGRDIIHLQIGEPDF
                     DTPKNIVDAGAEALRSGWTHYGPANGDPELRRVIADYIHRSRGVDYDAEQVIVTPGGK
                     PIMFFLILALLEAGDEAIFPDPGFPIYQSMIEFVGAKAVPISLREENDFRLDVDDLRG
                     LVTERTKLLIINSPANPTGGVLSEGDLQAIAGLAIENDLIVLSDEIYSELLYEGVHHS
                     IAALPGMAERTVILDGFSKTYAMTGWRLGYGLFPRNLVEPITTLGVNSVSCTSVAVQR
                     AGIEALTGPQDAVAEFREAFRRRRQLVVDRLNAIPGITCVMPKGAFYAFPNIAATGKS
                     SQEFADELLATYGVAAVPGTSFGAAGEGYIRISYANSEANLEKALGLIEQAAGAFIG"
     gene            complement(4032..5711)
                     /locus_tag="VGR29_11855"
     CDS             complement(4032..5711)
                     /locus_tag="VGR29_11855"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2074323.1"
                     /translation="MATGAGRDLGVISVRDIVHHGLPAGTTIVAGATGNGREVTWATR
                     LRPAPPAFGHISGGEIVFLPPGVLDLIDDRLNLADAIRQLATFGVAAVAITGEADSAA
                     CAAADAASVPLLVLPPTVDFGHLERETARAIAERRRVLQQHGQETGRKLMELAIAGES
                     LTDLTTEFSRLGQRAVALEGRDGGLLSFQSEPENLVTEETAQRILQQTNTYAQAWLRS
                     VGSSSAAEPPVHAWTTDGGWTRIVAPVTGRTGLLGNVSILVPAGQEGPEDSVLASRAA
                     AASAVALAREQAAASVRREVELNVLDEMLDGALRSEISLSQQAQRLGHDLNQEFCTLN
                     ARVDPAQAGPARSREGRWAVLEDGLRRAQRNAGLNMLWRVRNTSAEVVWPVTNAGSPA
                     EISGIVQEELTDALQSHGFTEVVSIGIGRPGHGIQGIRRSHQEARQALTLGRRLHGTG
                     HITDFDALGIYRLILAAEQLPELRAFQDEALGSLVSYDRVHRSNLTQTLEAFFAANCS
                     PKEAASILDVHRNTVLYRLDRISEITGLQLDDPDTRLRLHLALHIRLALDA"
     gene            complement(5711..7087)
                     /locus_tag="VGR29_11860"
     CDS             complement(5711..7087)
                     /locus_tag="VGR29_11860"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEV2074324.1"
                     /translation="MATHLDMELDSLTSAHEDIQGAMDALGEQSVMLVNLQFSDIAGG
                     IRTVTIPAALLPKVFERGYRFDGAAMAGGKRQVEIDLFLMPDPTTLTIFPPTNDSPRR
                     AQIFCWVTRRNGQPFAGDPRSALQRQLEAASQLGLDYRVGVELEFYLYKGDDLAHATL
                     LPGATSTGYFGDGEDTSAEARDAIVATLQELGVGVHGAHHETGPGQQELDLRHSGSVR
                     VADQIITTRQIIRQVASRHDMRATFMAKPFADAPGSGMHLFQRLLSYPAGEDLIPEIE
                     GEHDLSPTARHIIGGQLMHAAAMSAILCTTVNSYKRLADGHRAPTHATWARVSHGSLL
                     RLPAAPSGTCADIELRSPDAMANPYLAIAVALGTALDGIRKEYEPPSPLDENLVSYDD
                     DELNRLGVPRLPQTMGEALEALAASDAVRDILGAYIFDQLLSVKRAEWAEYRRHVSPW
                     EHMRYGDL"
     gene            7531..>9346
                     /locus_tag="VGR29_11865"
     CDS             7531..>9346
                     /locus_tag="VGR29_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEV2074325.1"
                     /translation="MESSQRTGHQQLSQAPLYDSAYEHDSCGVGLVVDIKGRPSRSIV
                     EKALAGLVNLTHRGGVGADARTGDGAGILLQIPHALFEETLIEAGASDLKAGEYGVAM
                     LFLPLDLSLRAAAIDAVMSAVAERGVDLLARRDVPVQVDMLSETAAATRPHVAQILVR
                     RPEGVDLGTFERDLMLLRRTMESRVAALGMSDADFFAASCSARTIIYKGFCLPEDLAR
                     FYPDLADPRMESAIALFHQRYSTNTQPTWGLAQPFRLIAHNGEINTVQGNRTWMRART
                     ADLVMSDGTTGAELEPAVAMTGSDSLSLDTALELLRHSGRSLPHSLMMTIPEPWEQLK
                     EMDADRRAFYDFHAGLQEQWDGPAALGFSDGVLAGATLDRNGLRPLRYAITEDGWFIA
                     GSEAGTVEIDQSRIVEKGRLGPGQMILVDTDAGVVLRNRELKQAVASNAPYAEWLRQG
                     RIPLDVAPEIDEPLPVPPEADAKTRAAMTKANPQQVAKQRAFGYTAEDIRLIVMPMAG
                     ERKEPTWSMGDDAPLAVLSEVPRPLTAYFRQRFAQVTNPPIDSLRERSVMALDTWLGP
                     RGNLLEKTPDHARLVHLRSLVLTASEFNAIASLDRLNIA"
BASE COUNT         1773 a         2770 c         2981 g         1822 t
ORIGIN      
        1 acgtaatcca tcgctgtgga ttgcgtcatg gagatcactg cgcccttggt ggtgccgtaa
       61 gcgaagcggc gggcaacggc aacctgtccg gcgatcgacg ccatattgat gatgcacccg
      121 ccctttgggt cctgggaaag catctgcttc acaccggcgt gggagcagtt gtagacacca
      181 tagacattga cggcaatcag gcgggcaaag tcttccggag acgtttcggt gagattgccc
      241 accatgccga taccagcatt gtttacgaga atatccaggg agccagtgcg atcgacaatc
      301 gacgctatgg cggactcggc ggagaccatg ttggtgacat caagccgaac gccgaacgct
      361 gcatcacccg cgtcggtaat ctcttcagca acggatgcgg cggccccctc gttgatgtcg
      421 ccgatcgcga ccgtcgcccc ctgttgacca aagagcacgg caatctcccg tccgattccc
      481 gaggcgcctc cggtcacgag tgcggtcttt ccgtcgagcc tgaacatcca ctctccccgg
      541 attgtgagcg gttcaaaggt acgaattacg tcggcgaaca ccgactatca taccgattgt
      601 atacaaaatc gcaggtgctg catagcgaaa ttaaggctaa aggcggctgg ggaatcatgc
      661 gccgagggat ggatcaaagt tgaagcagtt ccagcgcggg actgtccgga tagagaagct
      721 cgactcgctc gcggagccat gcctgttgcc tttgcgagag ccgagggatc atccgccgaa
      781 ggtcatcggc gtccctggaa caatgtggaa cgcgtgactt gtagaggagc tggagctccg
      841 gtgcgatgat cgggatacca tcgcgtacat ccgagagctg ggcaatctcg ccggtgatcc
      901 ggctgtctcg ggggaatatc catcgatcgc cctccgtgcg gatgatcatc agctgaaacc
      961 gccacacgtc tgatgatggg gggcggcacc agatgtcccg ggtcccctcc ggaagtgtct
     1021 ccccctcgat ccagtgccgc agcgtgccgg gaggggcggc ggccataatc acccagccgg
     1081 gaagcaatgc atgaacgtgc tccaaatcgc gttgcaggac cagcacatct gtatcctcat
     1141 ggagtcgggt gggatgtccg agatagaggt ccagcgacca cccgcctgcg atccaccacc
     1201 gacctggata cactcggaag aggcttgcaa cggccctcgg cggcattgca ctccacggtc
     1261 tctcaatttc attcatggca tctaccctga aatgacgaga atgttggcga aaggtgtcgt
     1321 atcaccggtg cgaatcgtcg cgcgacccct gctactcagg cgtttgagct ccagatgtgg
     1381 cacggtctcg atccgaaggt cgggacggat ggcacgaagt tcgtcgacaa agcccggtga
     1441 aaagctctct gcctcctccg cgaggataat ccggtcgatc acgaactcgg tgtcaacgag
     1501 acggatcaca tcgaggactg ttggcaggtc atcagccagc gcgaggtcga tgcggtcctg
     1561 cgatgcaggg atggggaaac cgcgatcgca gatagtgaaa tagtccgtgt gccctgtgga
     1621 tgcaagcagg tgattgatcg ctgggttgag gatgccgccg cgcttcattc aaccggccct
     1681 gtctcctggc cggagtgcag ggcgtcacgt tgctccagcg tgatccgctc cctgaggtcg
     1741 ggtgcggtcc gtttgaagag atagtggccg gcagttggca aggccgcgac caactcccag
     1801 ccctctgcgc cgagctcgtt gagatgctgc tgggtcagcg gctcgtcgct gtgttcaacg
     1861 tgatactccc accggtcgtg atcactgctc atgttgcctc tcctgattcg tcttggacac
     1921 catcatcgaa cggggccagg tctgccagga cgcgtgcttt catgtccaac aggtgcgact
     1981 caagggcgct gtgggcaccg tcgatgtcgt cgtccacaag gcgctggatg atggttcgat
     2041 gctggtggag ggccagctcc atccatcccg gaagctctgc accggcttgc tggaaaatgg
     2101 agatttgcgc ccagagaccc gtaacgatgg agatcaaacg ccgattgccc gatttctcca
     2161 ccagccagct gtggaaccca cgatctgtct tgaggaacgc ttcggccgct gcctcacgtt
     2221 cgtcctccgt cgtggcgaac ctaatcagcc gctcggcgtg ctcaagctcg aagagcgccc
     2281 gctcatattc ctcctggtga tctgatcggg tgatctgacg gagcgcgaag gtttcgagcg
     2341 cggcacggag gtcgaagatt tcctcgagat ccttgcggga gaaggtcgca acgaaaaagc
     2401 cacgattcgg ttcggccacg acgacgccat cctgtaccaa ctgaaggagt gcctcccgga
     2461 taggggtccg gctgacgccc aactccgagc tgagacgaag atcggagagc ttctcgccag
     2521 ccctcagctc gcgcctcaag atcctgtcgc gcagcacccg atagacatcc gtgcccagat
     2581 tgcggcgctc gatctgcgac gcgggttcgg gtatgggctt tcgagcgtgc tggctcattg
     2641 ggggctccgt gggttggcta cgaacgcacc gcgtatgggt cgcgtggcgc gtcccccggg
     2701 agggactggt tgatcagcgt gtcatcctca gtcatggtgt atccgatata caatgcattt
     2761 cgtccattgg caagatgtgc gccgccataa tgtgaagagg gaagcgtgag atgatggttt
     2821 cattggcatc gcggatggaa cggctgggaa cagagacggc gttccaggtc atggcccgcg
     2881 cccgtgcgct ggaggcgcag ggcagggaca tcatccatct gcaaatcggc gagccggatt
     2941 tcgatacgcc gaagaatatc gtcgatgccg gtgcggaggc cttgcgctcc ggctggaccc
     3001 attacggccc ggcaaacggc gaccccgagc tacggagagt gatcgccgac tacatacatc
     3061 gctcacgtgg tgtggattac gacgccgagc aggtgattgt gacacctggc ggcaaaccga
     3121 tcatgttctt tctgatcctg gcattgctgg aagcgggcga cgaggcgatt ttccccgatc
     3181 ccggctttcc tatctaccaa tcgatgatcg agtttgtggg cgcgaaagcc gtgccgatct
     3241 cgctacggga ggaaaacgac ttccggctcg acgtggacga ccttcgcggg ctggtgacgg
     3301 agagaaccaa gctgctgatt atcaacagcc cggccaaccc gacaggcggc gtcctgtcgg
     3361 aaggtgatct gcaggcaatc gctggattgg cgatcgagaa cgacctgatc gtactgtcgg
     3421 atgagatcta cagcgagctg ctctacgaag gcgtgcacca ctcgattgcg gcattgcccg
     3481 ggatggccga gcgcacggtg attctcgacg gattctcgaa gacgtacgcg atgacgggtt
     3541 ggaggctcgg ttatgggctc ttccccagga acctggtgga gcccatcacg acgctgggcg
     3601 tcaacagcgt ttcgtgtact tccgttgcgg tgcagcgtgc cggcatcgag gctcttaccg
     3661 ggccccagga cgcggtagcc gagttccggg aggccttccg gcgacggcgg cagctcgtcg
     3721 tggacaggct caacgccatc cccggcatca cctgtgtgat gccaaagggg gcattctatg
     3781 ccttcccaaa tatcgcggcg acgggcaaga gtagccagga gtttgctgac gagttgctcg
     3841 ctacatacgg ggtcgctgcg gtacccggaa cgagctttgg cgctgctggc gaaggctaca
     3901 tccggatctc ctatgccaac tcggaagcga atctggaaaa agcacttggg ttgatcgaac
     3961 aggcggctgg ggctttcatt ggctagcgtc aggattggcc ggttcccgga ttgccatcgg
     4021 tctgtagctg gttatgcatc cagtgcgagc cggatgtgca gggcgagatg aaggcgaagt
     4081 cgtgtgtcgg gatcatccag ctgcaggccg gtgatctcgg aaatgcgatc cagccggtag
     4141 agaacggtat tccggtggac atccaggatc gatgcggctt ctttcggact gcagttggcc
     4201 gcgaagaacg cttcaagtgt ctgggtgaga ttcgagcggt ggacgcgatc gtaggagacg
     4261 agggaaccga gcgcttcatc ctggaatgct cgcagctcgg gcaattgctc ggcggcaaga
     4321 atcagccggt agatgcccag ggcatcgaaa tccgttatat ggccagtgcc atgcagccga
     4381 cgccccaggg tgagcgcctg acgtgcctcc tgatgcgagc ggcggatgcc ctggatgcca
     4441 tggcccggcc ggccaatacc gatggagacg acttccgtaa atccgtgcga ctgcaacgca
     4501 tcagtaagct cctcctggac aatgccggaa atctctgcgg gagagccggc gttggtcacg
     4561 ggccagacaa cctcggcgct ggtgttccgt acgcgccata gcatgttgag gccggcgtta
     4621 cgttgcgccc gtcgaagccc atcctcaagc accgcccatc ggccctcccg tgagcgggca
     4681 ggtccggcct gggcaggatc cacccgggcg ttaagcgtgc agaattcctg gttcaagtca
     4741 tgaccgaggc gctgtgcctg ctgggagagc gagatctcgc ttcgcagggc gccgtcaagc
     4801 atttcgtcca atacgttgag ctcaacctcg cgccggacgc tcgcagctgc ctgctcccgg
     4861 gcaagggcaa cggcgcttgc ggccgcagcc cgggacgcga gaaccgaatc ctccgggccc
     4921 tcctgacccg ccggaaccag gatcgagaca ttgccgagca ggccggttcg cccagtgact
     4981 ggcgcgacga tccgggtcca accaccgtcg gttgtccagg catggacggg tggctccgcc
     5041 gcactggacg agcctaccga gcggagccat gcctgagcat aggtgttggt ttgctgcagg
     5101 atacgctgtg cggtttcctc ggtgaccagg ttttccggct ccgactggaa gctgagaagt
     5161 ccgccatccc gaccctccag ggcaaccgcc cgctggccga gccggctgaa ctcagtcgtg
     5221 agatcagtga gcgattctcc agcgatggcc agctccatga gcttgcggcc ggtttcctgg
     5281 ccatgctgct gaagtacccg tcgacgctct gcgattgcgc gcgctgtttc acgctcaagg
     5341 tggccgaaat ccaccgtggg aggcagcacg agcaggggga cggaggctgc gtcagcggca
     5401 gcgcaggcag ccgaatccgc ctcgccagtg atcgcgactg ctgccacgcc gaacgtcgca
     5461 agctggcgta tagcgtccgc aagattcagg cgatcatcaa tcaggtcgag cacaccgggt
     5521 ggcaggaaga caatttcgcc acccgaaatg tgaccgaatg caggcggcgc aggtctgagc
     5581 cgggtggccc atgttacctc gcgaccgttt cctgtggccc cggcaacgat cgtggtgccg
     5641 gccgggagtc cgtgatgaac gatgtcgcga acggagatca ccccgagatc gcggcctgcg
     5701 cctgtggcca ctagaggtct ccgtagcgca tgtgctccca cgggctgacg tggcggcgat
     5761 actccgccca ttcagcccgt ttcaccgaga gcagctggtc gaagatatag gcgccgagga
     5821 tgtcacggac ggcatcgctt gcagcgagcg cctcaagtgc ttcgcccatc gtctggggga
     5881 ggcgtgggac gccaagccgg tttaactcgt cgtcatcgta ggagacgagg ttttcgtcga
     5941 gcggtgaggg cggttcgtac tccttacgga taccgtccag tgcagtaccg agagcgaccg
     6001 cgattgccag gtaggggtta gccattgcat cgggactgcg gagttcaata tccgcgcatg
     6061 tgccggaggg tgccgcgggc agccgcagga gtgacccgtg gctaacccgt gcccatgtcg
     6121 cgtgggttgg cgcccggtgc ccatctgcca ggcgtttgta tgaattgacc gttgtgcaga
     6181 ggatcgcgct cattgccgcc gcgtgcatca gctggccacc gatgatgtga cgagcggtcg
     6241 gcgaaagatc atgctccccc tcgatttcag ggatcaagtc ctcacctgcc ggataggaaa
     6301 gcaatcgctg gaacaggtgc atacccgatc ccggtgcatc ggcgaaaggc ttggccatga
     6361 aggttgcacg catgtcatgt cgactcgcga cctgccgaat gatttgccgg gtcgtgatga
     6421 tctggtcggc gacgcggacg cttccgctgt ggcggagatc gagttcctgt tggcccggtc
     6481 ctgtttcgtg atgcgctcca tggactccaa cgccgagctc ctgcagcgtg gcaacgatcg
     6541 catcgcgcgc ttcggcgctc gtatcctctc cgtccccgaa gtaccccgta ctggttgctc
     6601 ccggaaggag agtggcgtgt gccaggtcat ctcccttgta gaggtagaac tcgagctcga
     6661 cgcctacccg gtaatccagg cccagctggg acgctgcctc gagttgtcgc tgcagggcag
     6721 agcgtggatc tccggcgaaa ggctgcccgt tgcgccgggt gacccagcag aaaatctgcg
     6781 cgcggcgcgg gctgtcgtta gttggcggga agattgtcaa cgtcgtggga tccggcatga
     6841 ggaagagatc gatctccacc tgccgtttgc cgcccgccat cgcggcgccg tcaaagcgat
     6901 aaccgcgttc gaagaccttg gggaggagcg ctgccgggat ggtgaccgtc cggatgccgc
     6961 cggcgatatc gctgaactgc agattgacga gcatgacgct ctgctcgccc agtgcgtcca
     7021 tagcgccctg gatatcttcg tgagcggacg tcagagaatc gagctccatg tcgagatgtg
     7081 ttgccatcaa gggaccctct aaaaactggg aagcatcggc tgtgcgactg cacaaaaggg
     7141 cagctcatca ccatgactcg ccgtccccgt attggccgca ccttgcggta aatccatgct
     7201 cacgtgccat gcaggtgcca tttacgcgcc agtatagagc ttacggacgc actttgtgcg
     7261 cagaacccaa acgtctccct ctgtcttctt ggcgttgtgt acctgtgact cgatttggat
     7321 gaaagataga ctaaagagtc ccacgatgca gtggaatagc gaacgatgtg gtcccgtacc
     7381 agtcactgaa gaatgatggt gatgacaata actggatcga tacgcgctgg ggttcgtgtc
     7441 tgatttcacg atttaggcgc aagaaccatt gccttaacaa acgtcgttcg ccgttctgtc
     7501 ccttgggccg gcggcaacag ggagtgttct atggaatcat cgcaacggac tggtcaccag
     7561 cagttgtcgc aagcaccact ctatgactcg gcctacgagc acgactcctg cggggtcgga
     7621 cttgtcgtag acatcaaggg tcgtccgtcg cgctcaattg tcgaaaaggc gctcgccggg
     7681 cttgtgaacc tgacgcatcg cggtggggtt ggcgcggacg cccgtacggg cgatggcgcg
     7741 ggtatcctgc tgcagattcc gcatgcgctg tttgaggaga cgctcatcga ggctggcgca
     7801 tcggatctca aggcgggcga gtatggcgtg gcgatgctct tcctgccgct ggacctctcc
     7861 ctgcgggccg ccgctatcga cgcggtcatg agtgcggtcg ccgagcgggg agttgatctg
     7921 cttgcccgcc gggatgtgcc tgttcaggtc gatatgctga gtgagacggc cgccgcaacc
     7981 agaccacatg tcgcgcagat cctcgttcgg cggccggagg gtgtcgatct gggcacgttc
     8041 gagcgggacc tgatgctgtt gcggcgaacc atggagagcc gggtcgcggc cctgggcatg
     8101 tcggacgccg acttctttgc ggcatcctgt tcggcccgga cgatcatcta caagggattc
     8161 tgcctgccgg aggacttggc ccggttctat cccgatcttg cggatcctcg gatggaatcg
     8221 gccattgcgc tcttccatca gcgctattcc accaatacac aacccacctg gggtctggcc
     8281 cagccgttca ggctcatcgc ccataatggc gagatcaaca cggttcaggg caaccggacc
     8341 tggatgcggg cccgcacggc ggacctcgtg atgagcgacg ggaccactgg tgccgaactc
     8401 gagcctgccg tcgcgatgac cggcagtgat tcactcagcc tggataccgc gctcgagctg
     8461 cttcgccaca gtggccgctc cctcccacac tcgctgatga tgacgatccc ggagccatgg
     8521 gaacagttga aggagatgga cgccgatcgg cgggccttct acgactttca tgcaggcctg
     8581 caggagcaat gggacggtcc agccgcactc ggcttcagcg atggtgtgct ggccggcgcg
     8641 acccttgatc gcaatgggct ccgtccattg cgctacgcga tcaccgaaga cggatggttt
     8701 atcgctggat ccgaggctgg cactgtcgag attgaccagt ccaggatcgt tgagaaaggg
     8761 cggcttggcc cgggccagat gatcctcgtc gacaccgatg ccggtgtggt gctccgcaac
     8821 agggagttga agcaggcggt cgcttcgaat gcgccgtatg cggaatggct gcgtcaggga
     8881 cggatcccgc tggatgttgc tccggagatc gacgagccac tgccggtccc gccggaggct
     8941 gatgcgaaga cccgcgcggc aatgacgaag gctaatccgc agcaggtcgc gaagcagcga
     9001 gccttcggct atacggccga ggatatccgc ctcatcgtga tgccgatggc tggcgagcgc
     9061 aaggagccga cgtggtcgat gggtgacgac gcgccgcttg cggtgctctc agaggtgccg
     9121 cggcctctga cagcctattt ccggcaacgg tttgcccagg tgaccaaccc gccgatcgac
     9181 tccctccgtg agcgctcggt catggcactc gacacctggc tcggcccacg tggcaatctg
     9241 cttgaaaaaa cacctgatca cgcccggctc gtccatctgc gcagccttgt gctcaccgcc
     9301 agtgagttta atgcaatcgc ctcgctcgac cggctcaata tcgcgc
//
LOCUS       DASYWM010000294         1900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_295613, whole genome shotgun sequence.
ACCESSION   DASYWM010000294 DASYWM010000000
VERSION     DASYWM010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295613"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1162)
                     /locus_tag="VGR29_11870"
     CDS             complement(143..1162)
                     /locus_tag="VGR29_11870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074326.1"
                     /translation="MTDVPRAGQPVMTLRRFTISPRLSHIGQTYGPLAALVALFLFNA
                     FFTDRFLTMQSLRLNLSQVSTIIIVGVGMTLVIATGGIDLSVGSLMAIGGAIAPLIFM
                     SDQGPLSMYAPLTNVIALVAALVVAALFGLFNGVLVAKFQIQPIIATLVLFIAGRGIA
                     QVITNGSIQTFRNPDFQYIGLGTPLGIPFQAFIALAVVLFSAWVVKSTTFGRYLLATG
                     GNEAAARLAGVPVNRIKLIVYVVSGTLAGLAGLIVISINSSSDANLVGLDMELNAIAA
                     VAVGGTPLTGGRANVVGTLIGALILQLIRTTLISHNIQDAVARVVTAAAIVFAVLMQR
                     GRSTG"
     gene            complement(1159..>1900)
                     /locus_tag="VGR29_11875"
     CDS             complement(1159..>1900)
                     /locus_tag="VGR29_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2074327.1"
                     /translation="EAQGLKRGRALRDASVTVNAGEIVGLAGLLGSGRTEVARAIFAA
                     DRIEDGDIRLEGEPANFCSPIDAIRAGIGFSSEDRKSEGIIPYLSVQENMTLAALPSL
                     SQHGIVSKEKQRELVNRFIQRLGIRTSGPDQKIRELSGGNQQKVLLARWLCLNPKLLI
                     LDEPTRGIDVGAKAEIQTLINEFADNGLGVLMISSEIEELAEGSDRVVVLRDGRTVAE
                     MPRSEISEDAIMNAMAHGTDEGVTGATA"
BASE COUNT          386 a          554 c          609 g          351 t
ORIGIN      
        1 gaagagaatc agggtcacca gtgcggcgag ggctccctgt cgctgaagaa tacttgccgt
       61 tcgcgcccag taggaagcct gttgctcttg ccggatatcg gtggttaccg gtgtattcat
      121 gctcgttgaa ccattcctcc ggttatccgg tcgaccgtcc tcgttgcatc aggacggcga
      181 agacaatcgc ggcggcggtg accacgcggg ccacggcgtc ctggatgtta tgggagatca
      241 gggtggtgcg aatcaactgg agaatcagcg ccccgatcag ggtgccgaca acgttggccc
      301 ggccgccggt gagtggcgtg cctcccacgg caaccgccgc tatggcgttg agctccatat
      361 cgagaccgac gaggtttgcg tccgatgatg aattgatgga gatcacgatg aggcccgcaa
      421 ggccggccag tgttcccgag accacataga caatgagctt gatcctgttg accggtactc
      481 ccgcgaggcg cgccgccgcc tcgttgccgc cagtcgccag gagatagcgg ccgaaggtgg
      541 tgctcttcac tacccaggcc gagaagagga cgaccgcgag ggcgataaag gcctggaagg
      601 gaataccaag gggcgtgccc aacccgatgt actggaaatc aggattgcga aaggtctgga
      661 tggatccgtt agtgatcacc tgggcaatgc cgcgcccggc aatgaacagc acaagtgtgg
      721 caatgatcgg ctggatctgg aatttcgcga cgagtacgcc gttgaagagg ccgaacagcg
      781 cagctacaac aagagcggca accagggcga tgacgttggt gagtggagcg tacatcgaga
      841 gtggaccctg atcgctcatg aagatcaggg gggcgatcgc tccaccaatc gccatgaggg
      901 agccgacgga gagatctatg ccgcctgtcg cgatgacaag cgtcatgccg acgccaacaa
      961 taatgatggt cgagacctgg ctcaggttca gccggagtga ctgcatcgtc aggaaccggt
     1021 cagtgaaaaa ggcattgaag aggaacaggg cgacgagggc ggcaagcggc ccgtaggtct
     1081 ggccgatgtg actgaggcgg ggagagattg tgaaacggcg cagtgtcatg accggttgac
     1141 ctgcccgcgg aacatcagtc atgccgttgc tcctgtcacg ccttcatcgg tgccatgagc
     1201 catcgcattc atgatcgcgt cctcactgat ctcgcttcgt ggcatctcgg cgaccgtgcg
     1261 gccgtcccgg aggacaacca cccggtcgct gccttcagca agctcctcga tctccgacga
     1321 gatcatgaga actccgagcc cgttgtcggc gaactcgttg ataagtgtct ggatttccgc
     1381 cttggctccg acatcgatgc ctcgcgtcgg ctcgtcgaga atgagcagct tggggttaag
     1441 gcagagccag cgcgccagca ggaccttctg ctggttgcca ccggagagct cccggatctt
     1501 ctgatccggc cctgaggtgc ggatgccgag gcgctggatg aaacggttga ccagctcccg
     1561 ctgcttctcc ttggagacga taccgtgttg ggagagcgaa ggaagcgccg cgagcgtcat
     1621 gttctcctgg acggagaggt aggggatgat gccctcgctc ttccgatcct cagagctgaa
     1681 gccaatgccg gcgcggatcg cgtcgatcgg agagcaaaag ttggctggtt cgccctcaag
     1741 gcggatatcg ccgtcctcga tcctgtctgc cgcaaagatg gcccgagcga cctcggttct
     1801 cccggacccc agcagacccg cgaggccaac gatctcaccc gcattcaccg tgacgcttgc
     1861 gtcgcgcagc gcccgtccac gtttcagccc ctgtgcctca
//
LOCUS       DASYWM010000295         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_446161, whole genome shotgun sequence.
ACCESSION   DASYWM010000295 DASYWM010000000
VERSION     DASYWM010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446161"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(546..>2224)
                     /locus_tag="VGR29_11880"
     CDS             complement(546..>2224)
                     /locus_tag="VGR29_11880"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV2074328.1"
                     /translation="VEAMVELAHEAAEIAHRFGGVMSGEHGDGLQRSELNELIFGTEL
                     YGVMCDLKTLFDPSGIMNPGKKVNAPSMTEHLRYGPTYHPLQIRTKMDFSREGGFIGA
                     VEACNGAAVCRKTHAGTMCPSYMATLDENDTTRGRANALRNALAGEILSPKDFANDAT
                     YETLDLCISCKACKTECPSSVDMAKIKTEFLAHYHDRHGTPLRARMMGHIHTLSKVAA
                     PVAPVANFMIQTPLTTPVMSMLGIHKDRKLAPFERRTFLRHWRAHRQRTPVAPITRGK
                     VVYFHDTFTTYNYPNIGMATVKLLEAAGFEVLIEQRRACCGRPMLSKGLIDAARKSAR
                     KNVSVLAEYAKKGIPIVGSEPSCILTLRDEYRDLLPDDEDVTVTASQTYMIDEFLAKL
                     EADGDLGIPWKTSTGPEVFFHGHCHQKALIGVEPSMAILRASGCRPTESGAGCCGMAG
                     SFGYETEHYDVSRKIGEERLFPAIEKTAMGVQISVAGVSCHQQIEHFTERGTHHIAEV
                     LAERIDPKQRWQPKVKAPTDVPEMDDPAVQDSDPTDEGTAHAKNQYEGPA"
BASE COUNT          384 a          714 c          661 g          465 t
ORIGIN      
        1 tatcgtcact gatcgctcgc cattgtcatc gatcagcact ccggtcccgg tggctccctg
       61 aggatcctcg ccgcataccg ggcgatcaca aacctgtggg attgtcgcga acccatgtgg
      121 cacggcgacc aagaaccgaa gcgtgtgctc atgcatgccg cacaacccat gcgagggata
      181 taaaggcgac atgccggtga atgtttgcca aatcctatca cggttagggc attcaggggg
      241 tagtctggtc ccgatctttc gacgagcagc acctgtgact gattgacaca tccgggttcg
      301 cctgatattg tacgtacacc tcagccttcc cttctgagga cccaggtgac gaccgcggag
      361 ctccatccct ctgcggtcga aacctggttt tttcttgcca ccgggcgtcc gcccccctcc
      421 ccgccgtcgt gtccgcgtca gtctcctgca acagattgca tctcttgctg aggcagagcc
      481 gcgtgcccgt cccacccagg caaacggccc ctggccccgt gacggtggct ttcgttgccc
      541 aactcctatg ccgggccctc gtactggttc ttggcatgcg cggtgccctc gtcggtcggg
      601 tcggaatcct gcacggcggg atcgtccatc tccggcacgt ccgtgggggc ctttaccttc
      661 ggttgccacc gttgcttcgg atcgatccgc tccgccagca cttcggcgat gtggtgagtg
      721 ccacgctccg tgaagtgctc gatctgctga tgacaggaga ccccggcaac ggagatctgg
      781 acgcccatcg cggtcttctc gatggccggg aagaggcgct cctcgccgat cttgcgcgag
      841 acatcgtaat gctccgtctc atagccgaac gagccggcca tgccgcagca cccggccccg
      901 ctctcggtcg ggcggcagcc tgacgcgcgc aggatcgcca tcgacggctc gacgccgatc
      961 agcgccttct ggtgacaatg cccatggaag aagacctccg gccccgtgct tgtcttccag
     1021 ggaataccga gatccccgtc ggcttccagc ttggccagga actcatcgat catgtaggtc
     1081 tgggaggcgg tgaccgtgac gtcctcatcg tccgggagca gatcacgata ctcgtcccgc
     1141 agcgtcagga tgcagcttgg ctccgatccg acgatcggta tgcccttttt cgcgtattcc
     1201 gccagcacgc tgacgttctt gcgggcactc tttcgcgcag catcgatcag gcccttggag
     1261 agcatcggcc gcccacagca ggcccgccgc tgttcgatga ggacctcgaa cccggcggcc
     1321 tccagcagct ttaccgtcgc cataccgata ttcggataat tataggtcgt gaacgtgtca
     1381 tggaagtaga cgaccttacc ccgcgtgatc ggtgcaacag gtgtgcgctg ccggtgggcg
     1441 cgccagtgcc gaaggaaggt ccgcctctca aagggcgcga gcttgcgatc cttatggatg
     1501 ccgagcatcg acatcacggg cgttgtcagc ggtgtctgga tcatgaaatt cgccaccggt
     1561 gcaaccggtg cggcgacctt ggacaaggtg tgaatgtgac ccatcatccg ggcccgtagc
     1621 ggcgtaccgt ggcgatcgtg atagtgcgcg aggaactcgg tcttgatctt cgccatgtcg
     1681 acgctggaag gacattccgt cttgcaggcc ttgcaggaga tgcagaggtc cagcgtctca
     1741 taggtcgcat cgttcgcgaa atccttcggc gacaggattt cacccgcgag tgcattccgg
     1801 agtgcattcg cgcggccacg ggtggtgtca ttctcatcca gcgtggccat atagctgggg
     1861 cacatcgttc ccgcgtgtgt cttccggcac accgccgcgc cgttgcaggc ctcgaccgcg
     1921 ccgataaagc cgccctcgcg gctgaagtcc atcttcgtcc tgatctggag gggatggtac
     1981 gttggtccgt agcgcagatg ctccgtcatc gagggggcgt tgaccttctt gccagggttc
     2041 atgattccgg acgggtcgaa gagcgttttc aggtcgcaca tgaccccata gagctcagtg
     2101 ccgaagatga gctcgttaag ttctgagcgc tgaagcccgt caccatgctc tccactcatg
     2161 acgccaccga agcggtgggc gatctccgca gcctcatggg caagttccac cattgcctcg
     2221 acgc
//
LOCUS       DASYWM010000296         4228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_389691, whole genome shotgun sequence.
ACCESSION   DASYWM010000296 DASYWM010000000
VERSION     DASYWM010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389691"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VGR29_11885"
     CDS             <1..429
                     /locus_tag="VGR29_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV2074329.1"
                     /translation="FVAYGIIAYLQGQTGVVTFCFIMAGALMGFLWFNAYPAQVIMGD
                     SGSLALGASLAVAAFMTGQWLLLPIVGAVFVGETVSVMIQTSWFKYTRKKTGEGKRIF
                     RMTPLHHHFERLGWSETQVALRFWLMGMMAGLLGCALALL"
     gene            448..1881
                     /gene="murD"
                     /locus_tag="VGR29_11890"
     CDS             448..1881
                     /gene="murD"
                     /locus_tag="VGR29_11890"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV2074330.1"
                     /translation="MSDHIAGTRYTVMGLGILGGGVGVAQYLARHGGMVTVTDMRDAS
                     DLQASIDLLTGLPIAYHLGGHDEADFTNSNADVVVRNPGVRRNNAFLALARESGVAIE
                     MEMSLFFRACRAPILGITGTKGKTSVSTLCSNMLRAWKSETVLAGNMGISALALVDDI
                     GPDQPVVIELSSWQLEALDEHQLGPSVAVLTNISEDHLDSYNGFDDYAGMKRTIGHHG
                     GQDAIVVYNYDDPECAKVVEETSSRLLPFGLSDPGIDGTWLAGDELVLRQGTGEKRVP
                     RPAQLQLSGEHGIRNALAAMAAVAAYGAPIEAIHTGLESFSGVPNRMEEVATIDGVLY
                     INDTAASAPAAAIAGLRVLSDRAKRVHLIAGGADKKTDLAPFADEIALRRPRVVLLDG
                     SATPELMRLLEARAVPYEGPFPDMGTAFAAAIREATAGDVVTLTPGCASFGMFRNEFD
                     RGEQFRSAVLALGQPGLDREMKGRMHV"
     gene            1892..3298
                     /gene="murC"
                     /locus_tag="VGR29_11895"
     CDS             1892..3298
                     /gene="murC"
                     /locus_tag="VGR29_11895"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV2074331.1"
                     /translation="MTANRTLETLPPPPATFHFIGIGGIGMSGLARILSIWGYKVTGS
                     DAAESAQTVSLRELGIPVVVGHHDPTFAGLADVVVTNKRAAANAKTELDAAEKSGARI
                     VKRGDLLGMVANARQSVCVAGSHGKSTTSGMLSVALRDLGADPTFAVGAILAATGTNA
                     EPGDGPHMVVEADEFDRSFHGLYPDVAIITSVAFDHPDIYDDQDAYDEAFLGFVHQIK
                     QGGTLIIAGDDPGAQRLLISVRDLGRDDLTVQTFGETGGVDWQLMRSGNGWSLVDPQG
                     EPHSLKLAVPGRHNARNAIAALAALHVFGFRIDEAAKALGAYTGIGRRFEHKGVVRGA
                     DVIDDYAHHPEEISAVLEAARDSYPGRRVIAVHQPHTYSRTYALMKEFAESLEQADVV
                     VLLEIYGVGEVNTHHISSAQLGSLIRKPVYLVEEPEETARRVLEILEPSDVVMTLGAG
                     TITRVGPLMIERTGEHVE"
     gene            3342..>4228
                     /locus_tag="VGR29_11900"
     CDS             3342..>4228
                     /locus_tag="VGR29_11900"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEV2074332.1"
                     /translation="MSPSDLPNKKIRVAVVFGGRSDEHDVSLRSARTIMDALNPDRYE
                     VVPMGITREGRWIAGEDPMAALIEVSPMFHLGEGEQKVEPTGERSIVPVVGAEALIPG
                     GVTDSVDVFFPALHGPMGEDGTIQGMLELAGLPYVGSGVLGSATAMDKAMTKVVMSQA
                     GIPQLPWMLVLRRDWEQNPEGVEAQIAEEIGFPCFIKPANLGSSVGITKAHNAMELTE
                     GLREAAHFDRRLVVEKGVVGRELEISVLGTDDPIVSVAGEIRPRGEFYDYNAKYIDDT
                     AELIIPAELEPEIQNQLEE"
BASE COUNT          784 a         1189 c         1381 g          874 t
ORIGIN      
        1 ttcgtcgcct acgggatcat tgcctatcta caaggccaga cgggcgtggt gacgttctgt
       61 ttcataatgg ctggcgctct gatgggtttc ctgtggttca acgcctaccc ggcgcaggtc
      121 atcatgggag attccggatc gcttgcactt ggtgcatctc tggcggtggc tgcatttatg
      181 acggggcagt ggcttttact gccaattgtg ggcgcggtgt tcgttggcga gacggtctcg
      241 gtcatgattc agacatcctg gtttaagtac acgcgaaaga agaccggcga gggcaaacga
      301 atcttccgca tgacgccact ccatcaccat tttgaacgtc tcggttggtc cgagacccag
      361 gtcgctctgc gcttttggct tatgggcatg atggcggggc tgctcggatg tgcccttgct
      421 ctcttgtagc gatggttgta gcagtcgatg agtgatcaca tcgcaggtac tcgctatacg
      481 gtcatgggac ttggcattct tggtgggggc gttggggttg cgcaatatct tgcccggcat
      541 ggcggcatgg tcacggtgac cgatatgcgt gatgcctcgg atctccaagc gtcgatcgat
      601 ctgctgaccg gtttgccgat cgcctaccat ctcggcgggc atgacgaggc cgatttcacg
      661 aactcgaatg ccgatgtcgt cgtgcgcaat ccaggggtgc gccgaaataa tgcattcctg
      721 gcactcgcgc gggagtctgg tgtggccatc gaaatggaga tgtcgctctt cttccgggcc
      781 tgcagggcgc cgatccttgg cattaccggc acaaagggga agaccagcgt ttcgacgctg
      841 tgctcgaaca tgctgcgggc ctggaagtcg gagacagtcc tcgcgggaaa catggggatc
      901 tccgcgctgg cgctggttga cgacatcggg ccggaccagc ccgtggtgat tgaattgtcc
      961 agctggcagc ttgaggcgct ggacgagcac cagctcggtc ccagtgtcgc cgtactcaca
     1021 aacatctcgg aggatcatct cgacagctac aacggttttg acgactatgc cgggatgaag
     1081 cgaacgattg gccatcatgg tgggcaggat gccatcgttg tctacaacta cgacgatccg
     1141 gagtgcgcaa aggtggttga ggaaacatca tcgcgactct taccctttgg gctctctgat
     1201 cctggcattg acggcacatg gctcgcgggt gatgagctcg tcctgcggca ggggactggc
     1261 gagaagcggg ttccccgacc agcccaattg cagctgagcg gcgagcacgg cattcgcaat
     1321 gcgctcgcgg cgatggctgc tgttgcggcg tatggagcac cgatcgaggc gatccacacc
     1381 gggcttgaat cgttcagcgg cgtacccaac cgaatggagg aggtcgccac gatcgacggg
     1441 gtgctctaca tcaacgatac tgcggcctct gcgcctgcgg cggcaatcgc cggcttgcgt
     1501 gtgctgagcg atcgcgccaa gcgggttcat ctgattgctg gcggagccga caagaagacc
     1561 gatcttgcac cattcgcgga cgagattgct cttcggcgtc cacgagtggt cctgctcgac
     1621 ggttcggcta caccggagtt gatgcgcctg ctggaggcgc gcgctgttcc ctacgaggga
     1681 ccgttccccg atatgggcac tgcctttgcc gccgcgatac gtgaggcgac agcgggtgac
     1741 gttgtcacgt tgacgccagg ctgcgctagc tttggcatgt tccgcaatga attcgaccgg
     1801 ggtgagcagt tccgttctgc cgtcctcgca ctgggccagc ctgggctgga cagggagatg
     1861 aagggccgaa tgcatgtttg aggatgtcgg cgtgacagca aaccgtactc ttgagacgct
     1921 tccgccacca ccggcaacat tccacttcat cggtatcggc ggtatcggga tgagtggcct
     1981 cgcccggatc ctgagcatct ggggctataa ggtcaccggg tccgacgccg cagaatctgc
     2041 ccagactgtg tcgctgcgcg aactcggcat cccggtcgtt gtcggacacc acgatcccac
     2101 cttcgccggt ctcgcagacg tggtggttac caacaagcgg gcggccgcca atgcgaaaac
     2161 ggagctcgat gcggccgaga aatccggcgc aaggatcgtg aagcggggtg acctgctggg
     2221 catggttgcg aacgcgcggc agtccgtctg cgtggctgga tcacatggca agtcaacaac
     2281 gtcaggcatg ctatccgtcg cgttgcgcga cctcggggct gatccgacat tcgcggttgg
     2341 cgcgatcctc gcggccaccg gaacaaatgc agagcctggt gatggccccc acatggtggt
     2401 tgaggccgac gagtttgatc ggtcgttcca cggcctttac ccggatgtcg ccatcatcac
     2461 gtcggtcgcc ttcgaccatc cggatatcta tgacgaccag gacgcttacg acgaggcgtt
     2521 cctggggttt gttcaccaaa tcaagcaggg cggaacgctg atcatcgctg gggacgaccc
     2581 gggggcgcaa cgtcttctca tttcagttcg agacctgggg cgggacgacc tgacggtcca
     2641 gacgtttggg gaaacgggtg gagtcgactg gcagttgatg cgttcaggga acggttggtc
     2701 gctcgtggat ccgcaggggg aacctcactc cttgaagttg gcggtaccgg gacggcataa
     2761 cgcgcggaat gcgatcgccg cgttggcggc gctccatgtg ttcggatttc gtatcgatga
     2821 agctgccaag gcccttggcg cctatacggg cattggacgc cggttcgagc acaagggcgt
     2881 tgtccgtggc gccgatgtca tcgatgacta cgcccaccat cctgaggaga tttccgcggt
     2941 gctggaggcc gcgcgcgata gttatccggg gcgccgggtg atcgcggtac atcagcctca
     3001 tacctactca cggacctatg ccctgatgaa ggagttcgcc gagtccctcg agcaggctga
     3061 cgtcgtcgtc ttgcttgaga tctacggggt cggcgaagtg aatacccatc acatctccag
     3121 cgcacagctt ggttcactga ttcggaagcc ggtttacctc gtggaggagc ctgaagaaac
     3181 ggcccggagg gtgctcgaga tccttgagcc aagcgatgtc gtcatgacgc ttggcgcagg
     3241 gacgattaca agggtcgggc cgctgatgat cgagcgcacc ggcgagcacg tcgagtgacg
     3301 aatatcgcga cgatcaatta cacccgaagg acgtttcctg catgagtccc agcgatctcc
     3361 ccaacaagaa gatccgcgtt gcagtcgttt tcggcggacg gtccgatgag catgatgtct
     3421 cgctgcgttc tgcccgaacg atcatggatg cccttaatcc cgatcgttac gaggtcgtcc
     3481 caatggggat tacccgggaa ggacgctgga tcgccggaga agaccccatg gcggccctga
     3541 ttgaggtctc gccgatgttc catctcggtg aaggtgagca gaaggtggag cccaccggtg
     3601 agcgctccat cgttccggtc gtcggggccg aggcgttgat accgggtggc gtgacggatt
     3661 cggtggacgt tttctttccc gcactccacg ggccgatggg cgaggacgga acgatccagg
     3721 gcatgcttga gctcgcaggc cttccctatg tgggtagtgg cgtgctcggc tcggcgacgg
     3781 cgatggacaa ggccatgacc aaggtcgtca tgtcccaggc ggggatcccc cagctgccgt
     3841 ggatgctggt gctacgtcgc gattgggagc agaacccgga gggtgtcgag gcacagatcg
     3901 ccgaggagat cggctttccc tgcttcatca agccggcgaa cctgggttcg agcgtaggaa
     3961 tcaccaaggc ccataacgcc atggagctta ccgagggact tcgggaagcg gcgcattttg
     4021 accggcggct ggttgtcgaa aaaggtgtcg ttgggcgaga gctcgagatc tcggtactcg
     4081 ggactgacga ccccatcgtc tcggtcgcgg gcgagatccg tccgcgcggc gagttctacg
     4141 actacaacgc caagtacatc gatgacactg ccgagctcat tattccagcc gagctggagc
     4201 cggagattca gaaccagctc gaggagta
//
LOCUS       DASYWM010000297         4247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_446493, whole genome shotgun sequence.
ACCESSION   DASYWM010000297 DASYWM010000000
VERSION     DASYWM010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446493"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VGR29_11905"
     CDS             <1..735
                     /locus_tag="VGR29_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEV2074333.1"
                     /translation="GGFCTSFPHRKRPFIFMNSSGVGGDVRTLLHEAGHAFHGFASSD
                     LPFIYQRMYGSEMAEVASMSMELLSAPYLRKVDGGYYDDEDYARARIEHLEGIISLLT
                     WVATVDAFQHWIYTDAAAADRDARDAKWIEIWSRFDPNTDWSGLEQERTARWYKQLHI
                     FLYPFYYIEYAIAQLGALQVWRNALHDQAKAVEDYRGALALGGSRPLPELFGAAGARL
                     IFDAEGMAELVELIEGELDSVERIGA"
     gene            1092..1802
                     /locus_tag="VGR29_11910"
     CDS             1092..1802
                     /locus_tag="VGR29_11910"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEV2074334.1"
                     /translation="MASVLINILIPIAILLTLSGEDRLGPIPALLLAVGIPVLFGIYT
                     LQRSRRVNISTVVGIVSVLLTGLIGVLRLDAQLFAIKEAAVPIVFALLIVISDRTRFP
                     IVRLLANQVVMRDRVERALDARGTHRQFRHHLTHTGRFWAGIMTLSGIVKFFLATWIV
                     TSPSGTIEFNRDLAQFQAVQVPTSTTLTMILMLALLAFLVRGTQRATGLPPRQIFKGG
                     ERMASFFGRFGRAQHSST"
     gene            complement(2000..2212)
                     /locus_tag="VGR29_11915"
     CDS             complement(2000..2212)
                     /locus_tag="VGR29_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074335.1"
                     /translation="MKQENPQQAYFRVTDSVPEQTWYWLAIASILASAGFKMANKDHW
                     ALFVGQWPPTFILFALFHRMLRPSGS"
     gene            complement(2439..3368)
                     /locus_tag="VGR29_11920"
     CDS             complement(2439..3368)
                     /locus_tag="VGR29_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074336.1"
                     /translation="MSVVNTSSTSDITQDGALAGAAEPGATRAPGRRPWWHTLFRQKL
                     ALMGASIVGFFALVALFGPLFAPFGPTEQFSQLRLTGPSQNNPFGSDEFGRDILTRLL
                     YGARISFQVGVIAVGISAAIGVVLGLVAGYFGGWIDNIVSLLMDVLFAFPTILLAIAL
                     MAMLGNSLGNVMIAIGLVNAPTFMRVVRGSVLSVRQMTYIESAVSVGASTGTILRRHV
                     FPNITAPLIVHASLNFATAVLAEASLAYLGLGNKPPSPSWGSMVSSSYGFLQLAPWAA
                     IAPGLAIGITVLGFNLLGDGLRDALDPRLRNLS"
     gene            complement(3402..>4247)
                     /locus_tag="VGR29_11925"
     CDS             complement(3402..>4247)
                     /locus_tag="VGR29_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074337.1"
                     /translation="RVLLGADARSTPEQIANIRRSYGLDQPLPVQYLKWLGHVLTGDL
                     GQSLRTGRPLTQELSMRLPVTIQLASFAAVLGMIPALLFGVLAAVKRNSRLDWMTTLT
                     ALAGISVPNFFLATLLVLLFSYKLRWLPNVGYKSFSDAPLDNLQLMILPAASLALPFM
                     AVLMRFTRSAVLEVLGQDYVRVARAKGVAQSRVILRHVLPNAGIPVLTIAGIQVASLL
                     GGTVIVEQIFALPGVGRYIYEAISNRDYPVVQSVTLVMAAIFVLVSLIVDVLYAVLDP
                     RLRNR"
BASE COUNT          885 a         1304 c         1291 g          767 t
ORIGIN      
        1 ggcggctttt gcacgagctt cccccatcgc aagcggccct tcatcttcat gaactcttcg
       61 ggcgttggcg gcgatgtccg caccctcctc catgaggcag gtcacgcctt ccacgggttc
      121 gcctcgtcgg acctgccctt catctaccag cggatgtacg gcagcgagat ggcggaggtc
      181 gcgtcgatgt cgatggagct gctttccgcc ccgtacctcc ggaaggtgga tggcggctac
      241 tacgatgacg aggattatgc ccgcgcccgt atcgagcacc tggagggcat catctccctc
      301 ctcacatggg tggccaccgt cgatgccttc cagcactgga tctacaccga tgcggccgca
      361 gccgaccggg acgcgcggga tgccaagtgg atcgagatct ggtcgcgctt cgaccccaac
      421 accgactgga gtggcctgga gcaggagcgc accgcccgct ggtacaagca gctgcacatc
      481 ttcctctacc ccttctatta catcgagtat gcgatcgcac aactcggtgc gctgcaggta
      541 tggcgaaacg cgcttcatga tcaggcaaag gctgtggagg attaccgggg cgccctcgca
      601 ctcggtgggt ctcgcccatt gcctgagctc tttggtgccg ccggtgcgcg actgatcttc
      661 gacgccgaag gaatggcgga gctagtggag cttatcgaag gcgagctgga ttcagtggag
      721 aggatcgggg cataggtgct gcttgcgaca ctcgagtgtt gggcacgtgg gagggcaaaa
      781 atcgcgatgg caagatcgta tccggatcgc cgcccgacag cgacgccaat agagtgggac
      841 gtggagcgcc ttagatggcc acgcaaaact gtttggccgc tggccatcta ggcagtcttg
      901 acacacgcca gactgcctag actgccatat ttctgagtat agagggggtg gccgcctgaa
      961 gaggtttggt caccatcccg gcacggagag ggaactgtca ggccagacca ggagccatcg
     1021 accgtcagct acaataatcg ctgcacaccc gtgtgacagg gcttccttct tcaggagaca
     1081 tcttccccat catggccagc gtcctgatca atatcctcat cccgatcgct atcctgctga
     1141 ccctgagtgg tgaagatcgg ctcggtccca tcccggcgct cttgctcgcg gtcggcatcc
     1201 cggtcctgtt tggcatctat accctgcaac gctcccgccg ggtcaacatc agcaccgtgg
     1261 ttggcattgt cagtgtcctg ctgacgggcc tcatcggcgt cctgcggctt gatgcgcagc
     1321 tcttcgcgat caaggaagcg gccgtgccga tcgtcttcgc gttgctcatc gtcatcagtg
     1381 atcgcacccg atttcccatc gtcaggttgc tggccaatca ggtcgtcatg cgcgaccgcg
     1441 tcgaacgggc acttgacgcc cgaggcacgc accggcagtt ccggcaccac ctcacgcaca
     1501 cgggccggtt ctgggccgga atcatgacgc tgtccggaat cgtgaagttc ttcctggcca
     1561 catggatcgt caccagcccc agcggcacga tcgagttcaa ccgcgacctg gcccagttcc
     1621 aggccgtgca ggtgcctaca tcgacgaccc tgacgatgat cctcatgctg gccctgctgg
     1681 ccttcctcgt gaggggaaca caacgtgcca ccgggctccc accgcgccag atcttcaagg
     1741 gcggtgagcg gatggccagc ttcttcggtc gctttggccg cgcacagcat agctcaacgt
     1801 aaggcttctg ccccagctct cctctgtagt gagaacctcc gatctccggg atcccggcga
     1861 cgcaatcgcg ctacatcact cgcctgccaa gattgcgttg tggggcgacc aaactactca
     1921 ttcgcacagt ggcgtgccac tgggacttaa actcaaacgc cctgccagac atctggcagg
     1981 gcgtttgatg tgatgcggac taggaaccgc ttggccgaag catgcggtgg aacagcgcga
     2041 agaggatgaa cgtcggtggc cactggccta cgaagagtgc ccagtgatcc ttgttcgcca
     2101 tcttgaatcc ggcggatgca agaatggagg caattgcgag ccagtaccag gtctgctcgg
     2161 gaacgctgtc ggtcacgcgg aagtatgcct gctgcgggtt ttcctgcttc attcgtatct
     2221 ctcctgatgt ggcaagtggt gcagttttca cgtcgaactg acgtcacaca ggagggttaa
     2281 gcacagtgag tgccacgagt tcgtcgacat cccgaagtct ctaggtacgg caacccagaa
     2341 agacgggtat gggagaagac cccatccagc gaaacttaca gctcaagatg ggacctgacg
     2401 gtgatggttg gctttcgtca agcacgaagc agtcggagct agctcaggtt ccggagccgt
     2461 gggtccaatg cgtcgcgcag cccgtcacca agcaggttga atccgagcac cgtgattccg
     2521 atggccaggc caggtgcgat cgcggcccac ggtgccaact ggaggaaccc gtaggaactc
     2581 gaaaccatcg atccccacga gggagaggga ggcttgttgc ccagcccaag ataggcgagt
     2641 gaggcctcgg caagcacagc cgtcgcaaag ttgagcgatg catgcacgat gagcggcgcc
     2701 gtgatgttgg ggaagacatg acggcgcaag attgtccccg tggacgcccc gactgaaacc
     2761 gcggattcga tataggtcat ctggcggacg gagagcactg acccgcggac gactcgcatg
     2821 aaggtgggcg cgttgacgag tccgatcgcg atcatcacgt tgcccagcga attgccaagc
     2881 atcgccataa gcgcaattgc gagaaggatc gtcgggaacg cgaagagcac gtccatcagc
     2941 agcgagacga tgttgtcgat ccaaccacca aagtacccgg ccaccagacc gagcactaca
     3001 ccgatcgccg ccgagatgcc cacggcgatg acgccgacct ggaaggagat ccgcgcgcca
     3061 tagagcagcc gggtcaggat gtccctgccg aattcgtcac ttccaaaggg gttgttctga
     3121 gacgggccag tgaggcgcag ttgtgaaaac tgctcggttg gtccaaaggg cgcgaacagg
     3181 ggcccaaaga gggccaccaa tgcaaagaaa ccgacgatgc tggcgcccat gagggccagc
     3241 ttttgccgga agagcgtatg ccaccatggc cggcgtccag gtgcccgcgt cgcccccggc
     3301 tccgccgctc ccgccagcgc accgtcctgg gtgatgtctg atgtcgagga cgtattcacc
     3361 acactcatgg agacgccaat ccctccggaa ctcgcacgtc gctaacgatt ccgcagccgc
     3421 ggatcgagaa ccgcatacag cacgtcgacg atcaggctca cgagcacgaa aatggcggcc
     3481 attaccagcg tcacgctttg gactacagga taatcccggt tcgagatcgc ctcgtagata
     3541 taacggccca cgcccggcaa cgcaaagatt tgctcgacga tcacggtccc gcccaacagg
     3601 gaggcgacct gaatcccggc aatggtaagg accgggatgc cagcattcgg cagcacgtgg
     3661 cggaggatca ccctgctctg ggcaacaccc ttcgcgcggg ccacccgaac atagtcctgc
     3721 ccaagcacct ccagcacagc ggaccgggta aatcgcatga ggacagccat aaacggaagc
     3781 gccaggctgg ccgccggcag gatcatcaat tgcaggttgt cgagcggtgc atcagagaac
     3841 gatttgtacc caacgttcgg caaccaccgc agcttgtatg agaaaagcag caccagcaat
     3901 gtggcaagga agaaattcgg gaccgagata cctgccagcg cggtcagcgt tgtcatccag
     3961 tcgagcctgg agttgcgctt gaccgcggcc agcaccccaa agagcagtgc cgggatcatg
     4021 ccgaggaccg ctgcaaagga cgccaactgg atggttacag gaaggcgcat ggaaagttcc
     4081 tgcgtcagcg ggcgaccggt tcgcagcgat tgcccgagat ccccggtcag gacgtggccg
     4141 agccacttca ggtactggac tggcaatggc tggtccaacc cataggagcg ccggatgttc
     4201 gcgatctgtt cgggggttga gcgcgcgtca gcacccagga gcacccg
//
LOCUS       DASYWM010000298         6868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_109073, whole genome shotgun sequence.
ACCESSION   DASYWM010000298 DASYWM010000000
VERSION     DASYWM010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109073"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VGR29_11930"
     CDS             <1..298
                     /locus_tag="VGR29_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV2074338.1"
                     /translation="INAAMLGGTRREKPAAYREASPLTRVDADSAPFLIIHGVRDEVV
                     PVEHSRRLVAALRGVDVEVTYGEYPRAYHLTTRDWRFSGPWALAFLGLHLRPEQ"
     gene            complement(728..1384)
                     /locus_tag="VGR29_11935"
     CDS             complement(728..1384)
                     /locus_tag="VGR29_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEV2074339.1"
                     /translation="MLAKVFSCAVVGLEGTLVEIEVDVGSRNPGMVLVGLPDAAVQES
                     RERVRSAIRNSGGKVPFGKITVNLAPADLKKAGPTYDLPIALGILISSNQLLTDVQDA
                     LVVGELSLDGHVRHTPGIISMLTMAANRGLRRAFVPEVDAREAALIEAVEVYPVRTLA
                     DLVNHLSGDVPIQRIDADPASFAAPDLSSLTDFADIRGQEHVKRGLEVAAAGAHNLIM
                     "
     gene            1567..1995
                     /locus_tag="VGR29_11940"
     CDS             1567..1995
                     /locus_tag="VGR29_11940"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEV2074340.1"
                     /translation="MNCQDEIVRGPVDLSDRAGSRAWETFSRGLLRRCPYSGGDNIFS
                     GWFTLKERCPHCQTLFAIEDGYFLGSYVINLGLTSIIAIAAVLWMIIWSDLSVVGMQI
                     SAVALAVLIPLFLYPYSLSLWMMLDLVIHPPGDFSRRHRT"
     gene            2114..3007
                     /gene="hisG"
                     /locus_tag="VGR29_11945"
     CDS             2114..3007
                     /gene="hisG"
                     /locus_tag="VGR29_11945"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEV2074341.1"
                     /translation="MTSARSIINGDNRLKLAIQRSGRLTDHTIALLKQIGLSFESYGQ
                     RLFSQTRNFPLSILYARDDDIPDYVELETVDLGIVGRNLVHEQNADVEEIHALGFGYC
                     QLVVAVPNDSGIESPEQLLNARIATSYPESARRYFEQLGGHPEIVTISGSVEVTPALG
                     VADAIVELTATGSSLQLNDLRSIHTILESEAVLVANKQAMADPAKRENIDRLLLRVRA
                     VDAANKYKYIMMNAPESALSSIRQVLPGLKSPTVVPLADPGWVAIHTAIKEDEFWEKI
                     EQLRVAGASEILVSSLDKFLL"
     gene            3031..4146
                     /gene="hisC"
                     /locus_tag="VGR29_11950"
     CDS             3031..4146
                     /gene="hisC"
                     /locus_tag="VGR29_11950"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV2074342.1"
                     /translation="MVTDLTDFVRFYLTPAAGYTPGASRLTDRPVIRLDWNESPWPLS
                     PTAQEVLVNFQRGNRYPDYSQAPLRKALGEYVGFDSSRIVAGAGLDDVFMTLAMLLIE
                     PGDEIVIADPTFGIYRSLFTLHGAVVRNVPLGPAPEFALDVDGMLDAVNEQTKLVILC
                     NPNNPTGHLFPREAIEQIVDGVSCPVAIDEAYAEFSGIDHLDLAESRNNVIVLRTLSK
                     FAGLAGFRVGYGIFPEVLVPYLQRVTPAFANITTLSAEIAIASLNDLDYLKANRDRIV
                     TERMRVIVALDAMAGVMPFPSATNFVLFRLPVADSTSVLDALARHDVFIRRYGTPGWG
                     LEDCLRVSIGTSVENDAFLAALSAVLQEQSALEEATT"
     gene            4143..4754
                     /gene="hisB"
                     /locus_tag="VGR29_11955"
     CDS             4143..4754
                     /gene="hisB"
                     /locus_tag="VGR29_11955"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEV2074343.1"
                     /translation="MSGQASNAREHSISRTTGETSIDIRLKLDGQGQVEVTTGVPFLD
                     HMVDALGRHGRLDIDLRATGDAAMDPHHTVEDTGILVGQALKAVLGERKGIERYGYAY
                     APLDEALARVVIDCSGRPFLDYNADMPEAVIGLDFAASLVEEFWRSVAMNAGLTMHID
                     LLRARNSHHAAEAIFKAAAVALRNAVRQTGLSDTVPSTKGVLA"
     gene            4751..5347
                     /gene="hisH"
                     /locus_tag="VGR29_11960"
     CDS             4751..5347
                     /gene="hisH"
                     /locus_tag="VGR29_11960"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEV2074344.1"
                     /translation="MIAVVDYGAGNLRSIKRALEVDGAETVVTANSDTIRSADRVVLP
                     GVGNAGAAMERLHDLGLVDVMKDVADAGTPLLGVCLGMQLLFGDQEEGPTVGLKLLQG
                     NARKLPSDHKIPHMGWNTVAFAPHSSLAGLGEASFYFVHSYVVYPTDVTDIAGTTDYG
                     VAFPSVVIRDNIWGTQFHPEKSGDSGLTLLKYWLAWNR"
     gene            5373..6095
                     /gene="hisA"
                     /locus_tag="VGR29_11965"
     CDS             5373..6095
                     /gene="hisA"
                     /locus_tag="VGR29_11965"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF010112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEV2074345.1"
                     /translation="MIVYPAIDIRGGQAVRLVEGDYDQETVYDADPVDAARRWAEGGA
                     EWIHIVDLDGARDGIRGNWEAISRIRAAVPVKLELGGGLRTMEDILAVADLGIDRMVI
                     GSAAIDNPALVSQAVQLYGDRIAVGLDARDGKLAAKGWLEQTETNAIDAAQRFVEAGV
                     CHFIFTDIRRDGKLIGPNLDALRKMIDSVQANVIASGGIGTNEDIRAVRETGAAGVII
                     GAALYKGTVQLADALRITAEEK"
     gene            6101..>6868
                     /gene="hisF"
                     /locus_tag="VGR29_11970"
     CDS             6101..>6868
                     /gene="hisF"
                     /locus_tag="VGR29_11970"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEV2074346.1"
                     /translation="MNGTSKGLTARIIPCLDVTDGRVVKGVEFVELRDAGDPVEMAAF
                     YNREGADELIFLDITASSDHRKTILDVVRETADQVFIPLTVGGGVRTVEDMRALLAAG
                     ADKVAVNTSALLNPDIIDQGAAQFGTQCIVVAMDAQQVPDEKRWEVYTHGGRTATGDD
                     ACEWAREAEERGAGEILLTSMNRDGTGRGYDLALLEAVSGAVNVPVIASGGAGSLEHF
                     HEALVPGRADAVLAASLFHFGTLRIGDVKRYLRGRGIP"
BASE COUNT         1405 a         2053 c         1965 g         1445 t
ORIGIN      
        1 gatcaacgcc gccatgctgg gtggaacccg gcgggagaag ccggcggctt accgtgaagc
       61 ttcgccattg acccgggtcg acgccgatag tgctcccttc ctcatcatcc acggcgtccg
      121 ggacgaggtt gtgcctgtgg agcattcgcg gcggctggtg gccgcgctgc gaggggtaga
      181 cgtggaggtc acctacggcg agtatccccg ggcctatcac ctcacgacga gggactggcg
      241 gttcagtggc ccttgggcac tggcgttcct cggccttcac ctgcgtcccg agcagtgagg
      301 cttcgtgtgg gatcggctag gccgtaagct gctcgtgttc ggttccggtg atgaagatcg
      361 ggtccagctc aaatgtagtg tgagcaaacc atcccaatgc gaccaggaga gcgctgtgat
      421 gcttacagcg accgtgtgag gcaaagctca tgcaatcgcc actgacaccc ggcacataat
      481 gcaccgttcg caggattccc gcttcccctt agtggcacgg cgtttctcgg aactgtcaac
      541 tgttttcgac gcaacaacac cggagggccg cacccccttt tcgctctacc cgcatgcggc
      601 atgcgattgt ctacgcctct gagcagcgcg agacctagat atcttgctac tcgccacctg
      661 gagctcgtag catcacttcc cgatcgggac agtggttcgc gcaaacttaa tgcaggcgtt
      721 ctgtccctta catgatgaga ttgtgagctc cggctgcggc cacctccaac ccccgcttga
      781 cgtgctcctg gcctcgaatg tctgcgaaat cagtgaggga cgagagatcc ggtgctgcaa
      841 aactggcggg gtcagcatcg atgcgctgga tgggaacatc tcccgaaaga tggttcacca
      901 gatcggcaag tgtgcgcacc gggtacactt ctaccgcctc gatgagcgcc gcctcgcgag
      961 catcgacttc tggcacgaat gcccttcgca gccctcgatt cgccgccatt gtcaacattg
     1021 atatgatacc gggcgtgtgc cggacatggc cgtccaatga cagctccccc actaccaagg
     1081 cgtcctgcac gtcagtcagg agttgattgg aggagatcaa aatcccgagt gcaatcggca
     1141 ggtcgtatgt tggtcccgcc ttcttgagat ccgctggggc aagatttacg gtgatcttcc
     1201 cgaacggcac ttttcctccg gaattacgga tcgctgaccg gacgcgctcc cgactttcct
     1261 gcacagcggc gtcaggcaaa ccgaccagca ccatgccagg gttcctcgac ccgacgtcta
     1321 cctcaatctc aacaagtgtc ccctccagac ccacgaccgc gcaactgaaa accttggcca
     1381 acactcgcga caccctcacc cttgagacac agcgacgagg agatcgccgg acacctcttg
     1441 tcccccgccc ggcttatgaa ccacaactgc tattcaactg gagttactgc cggaaatgta
     1501 tcagaattca atgaggcaga gtcgcctgtc ccctcctctc ctctcaccct ttcaggaccc
     1561 gtccatatga actgccaaga tgagatcgtc cgcggacccg tcgatctttc agatcgtgcc
     1621 ggcagccgcg cctgggaaac gttctcgagg ggactgctgc gtcgttgccc gtactccgga
     1681 ggggacaata tcttcagcgg atggttcacg ctgaaggagc gctgcccgca ctgccagacc
     1741 ttatttgcca ttgaggacgg ctattttctt ggttcatatg tcatcaatct cggcctcacc
     1801 tccatcatcg ccatcgcggc tgtgctctgg atgatcattt ggtcggatct gtcggtcgtt
     1861 ggcatgcaga tatcggcagt tgctctagcc gtccttatcc cactcttcct ctacccctac
     1921 tcgctgtcac tgtggatgat gctcgatttg gtcattcatc ctcccggaga tttctcccgg
     1981 cgacatcgca cctgaaggtg gatacgagcc gctccgcgtg ctacaagtga cacgtcgacg
     2041 cggacgccta ctccctgcga ggaggcgctg ttgtgatcga cgtcatcctc tccaataaca
     2101 ggaccacacc tgaatgacat ccgcacgttc gattatcaac ggcgacaatc gcctgaagtt
     2161 ggccatccag cgatccggtc gccttacgga tcacacaatc gccctcctga aacagatcgg
     2221 cctgtcgttc gaaagctatg gacagcgcct cttctcgcag acgagaaact ttccactgtc
     2281 catcctctac gcacgggatg atgacattcc ggattatgtg gaactcgaaa ctgttgacct
     2341 cggcatcgtg ggccgaaacc tggtccacga gcaaaacgcg gatgtggaag agatacatgc
     2401 cctcggcttt ggctactgtc aactagtcgt tgccgtgcct aatgattcag gcatcgaaag
     2461 cccggagcaa ctgctgaacg cacgcatcgc gacttcctac cccgaatctg cacggcgcta
     2521 ttttgagcag ctgggtggac acccggagat tgtaacaatc tccggttcgg tggaagtgac
     2581 gcccgcgctc ggcgtagcgg acgcgattgt cgaactaact gcgacgggtt cgtcactcca
     2641 gctcaacgat cttcgctcta tccacacgat cctggaatcc gaggcggtgc tggtcgcgaa
     2701 caaacaggcg atggcggatc cagccaagcg ggagaacatc gatcgactcc tcctccgggt
     2761 acgcgccgta gatgccgcca acaagtacaa gtacatcatg atgaacgcgc cagaatctgc
     2821 cctgtcgagt attcgccaag tgctcccggg cctgaaatcg cccacagttg tcccgctagc
     2881 tgaccccgga tgggtcgcga tccataccgc catcaaggaa gacgagttct gggagaagat
     2941 cgagcaactc cgcgtcgcgg gtgccagtga gatcctcgtc tcctcgcttg acaagttcct
     3001 gttgtaattc tccaaggaga tcgcctgccg atggtcaccg acctcacaga cttcgttcga
     3061 ttctacctaa cacccgcggc cggttataca ccgggcgcct ctcgccttac cgatcggcca
     3121 gtgatccggc tagactggaa cgaatctcca tggccgctct ccccaactgc ccaggaggtt
     3181 ctagtgaact tccaacgagg aaaccgctac cctgactaca gtcaggcgcc actgcgcaaa
     3241 gccttgggtg agtatgtcgg atttgattcg tccaggattg ttgccggggc tggcctcgat
     3301 gacgttttca tgactctcgc aatgctcctc atcgagcctg gtgatgagat agtgatagct
     3361 gatccaacct tcggcatcta ccggagcctc ttcacactcc atggcgcggt cgttcgcaat
     3421 gttccacttg gccccgcgcc ggagttcgcc ctcgacgtag acgggatgct cgatgcagtt
     3481 aatgagcaga ccaagctcgt tatcctttgt aaccccaaca acccgactgg ccatctcttt
     3541 ccccgggagg caatcgagca aatagtcgac ggcgtttcat gtcccgtcgc catagatgag
     3601 gcgtatgccg agttcagtgg cattgaccat ctagaccttg ccgaatcgcg aaataatgtg
     3661 atcgtgttgc gcaccttgag caagttcgca ggtctggctg gcttcagggt tggctacggc
     3721 atttttccgg aagttctcgt tccgtatctc cagcgagtta cccccgcgtt cgccaacatc
     3781 accacgctga gtgctgaaat tgccatcgcg tcgttgaatg atctcgacta cctcaaggca
     3841 aaccgtgatc ggatcgtaac tgagcggatg cgcgtaatcg ttgcgcttga tgcaatggcg
     3901 ggtgtcatgc cattcccctc ggccacgaac ttcgttctgt tccggctccc ggttgccgat
     3961 agtacgtcgg tgctggatgc acttgctcgg cacgacgtct ttatccgtcg gtacgggaca
     4021 ccgggatggg gccttgagga ttgcttgcgt gtcagcatcg ggacatccgt cgagaacgac
     4081 gcgtttcttg ccgctctttc agcggtactg caggagcaat ccgcactcga ggaagcaaca
     4141 acgtgagtgg gcaggcaagt aacgcacgcg agcacagcat ttcacgcaca actggcgaaa
     4201 cctcaatcga catccggctc aagctggatg gacaggggca ggttgaggta acaacaggcg
     4261 ttccgttcct tgaccatatg gtggatgcgc tgggtcgaca tggccgcctt gatatcgatc
     4321 tcagggccac cggggacgcc gctatggacc cacatcacac agttgaggac acgggaatcc
     4381 tggtcggcca agccctcaaa gccgtgctcg gcgaacgaaa aggcatcgaa cgatacggtt
     4441 acgcatacgc cccgctggac gaggcgttgg ctcgtgtcgt gatcgattgc tctgggcgac
     4501 ccttcctcga ctacaacgcc gatatgccag aagccgttat cggcctggat ttcgctgcca
     4561 gcctcgtcga ggagttctgg agatcagttg cgatgaacgc ggggctgacc atgcacatcg
     4621 atctcttgcg tgcccgcaac agccatcacg ctgcggaggc catcttcaag gcagcagcag
     4681 tcgcacttcg aaacgccgtg cggcaaaccg gtctaagtga caccgttcct tcaacaaaag
     4741 gcgtattggc atgattgcgg tcgtggatta cggagcaggg aatctccgtt caatcaagcg
     4801 tgccctcgag gtagacggtg cagaaaccgt ggtgactgcc aattcggaca cgatccgatc
     4861 ggcggaccgc gtggtactgc ccggcgtcgg caacgccgga gccgcgatgg aacgcctcca
     4921 tgatctcggc ctcgtcgatg tcatgaagga tgtcgctgac gcaggcacac cgctactcgg
     4981 agtgtgcctc ggcatgcagc ttctctttgg agaccaggaa gaaggcccca cggttgggct
     5041 aaaacttttg caaggaaatg cgcgtaagct tccgagcgac cataaaatcc ctcacatggg
     5101 ctggaataca gtcgcgttcg cgcctcattc ttcactcgcg ggtcttggcg aagcttcgtt
     5161 ttactttgtg cattcctacg tcgtctatcc gaccgacgtg accgatatcg ctggaacgac
     5221 tgattatggt gtcgcattcc cgagcgtcgt catccgtgac aacatttggg gcacgcagtt
     5281 ccacccggaa aaaagtggcg attcggggct gacgttgctc aagtattggc tggcctggaa
     5341 tcgctgatca gcactaactg gggacaagaa tcatgatcgt ctacccggca atcgacatcc
     5401 gcggaggcca ggcggttcgt ctggtagaag gcgattacga ccaggaaact gtctacgacg
     5461 ctgatcccgt tgacgcggca cgccgatggg cggaaggtgg cgcggagtgg atccatatcg
     5521 tggatcttga cggtgcccga gacggcatcc gtgggaactg ggaggcgata tcgcgcatcc
     5581 gtgccgctgt tcctgtgaag ctggagcttg gcggtggcct gcgaaccatg gaggatatcc
     5641 ttgcggtagc cgatcttggg atcgaccgca tggtgatcgg atcggccgct atcgacaatc
     5701 cggcgctggt ttcccaagca gtacaacttt acggcgatag gatcgctgta ggcctcgacg
     5761 cgcgggacgg caagctggcc gcgaagggat ggttggaaca aaccgaaacc aatgccattg
     5821 atgccgccca gcggttcgtg gaagcgggag tttgtcactt catcttcacc gacattcgcc
     5881 gcgacgggaa actgatcggg cccaatctcg atgcgcttcg gaaaatgatc gactcggtcc
     5941 aagcaaacgt tattgcatct ggcgggattg gcacgaacga ggatatacgt gcagttcgcg
     6001 aaacaggggc cgcaggcgta atcatcggcg ccgcactgta caagggcact gttcaacttg
     6061 ccgatgcatt acggattacc gctgaggaga agtagaaagc gtgaatggga cttcgaaggg
     6121 acttacggcc cggatcattc cctgtctcga cgtgaccgac ggtcgcgtcg tgaagggtgt
     6181 ggaatttgtg gagctccggg atgcgggaga tcccgtagag atggccgcct tctataatcg
     6241 ggaaggagcc gatgagttga tctttctcga catcacagcc tcctccgatc accgcaagac
     6301 gatccttgat gtcgtcaggg aaacagcgga tcaggtattc atccctctga cggttggggg
     6361 cggcgtccga accgttgagg acatgcgtgc gctgctggcc gctggggccg acaaggtggc
     6421 tgtcaacaca tccgcgctac tcaatccgga catcatcgac cagggcgcgg cacagttcgg
     6481 cacccagtgc atcgtcgtcg cgatggatgc tcaacaggtt cccgatgaga agcgctggga
     6541 ggtctatacg cacggtggcc gcaccgcaac cggcgatgac gcctgtgaat gggcccgtga
     6601 agccgaagag cggggagccg gcgagatcct cctgactagt atgaaccgcg acggcacagg
     6661 gcggggatac gacctggccc ttctcgaagc cgtctcaggt gcagtgaatg tccccgtgat
     6721 tgcctctggt ggcgctggca gcctggagca tttccatgag gccctcgtgc ccggccgggc
     6781 agacgccgtg cttgcggcct cgctcttcca cttcggcaca ctccgcattg gcgacgtaaa
     6841 gcgctatctc cgaggccggg gaatcccc
//
LOCUS       DASYWM010000299         6222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_371449, whole genome shotgun sequence.
ACCESSION   DASYWM010000299 DASYWM010000000
VERSION     DASYWM010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371449"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VGR29_11975"
     CDS             complement(<1..194)
                     /locus_tag="VGR29_11975"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEV2074347.1"
                     /translation="MCGRYVIEDFQELSETLRQVPFQIDYDPNPTWNAAPSQRLPVIV
                     EDDGAWHLRPMQWGLIPRWT"
     gene            complement(350..598)
                     /locus_tag="VGR29_11980"
     CDS             complement(350..598)
                     /locus_tag="VGR29_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074348.1"
                     /translation="MTAILINGLVVLMLLLFAAMALAPLLFSARSTAQVDHSTGEDRV
                     LHVSPVPMIERFRPNGGKQPLPLGKAPSTGDSSRRDAA"
     gene            1188..3038
                     /locus_tag="VGR29_11985"
     CDS             1188..3038
                     /locus_tag="VGR29_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2074349.1"
                     /translation="MKTLIRILGYLKPYRTWLITVYAALFIALGLQLSIPAVLGRAID
                     HGVIARDMSYLGKATLAIVGLATLQALFTFIRTYGTNVLAERVGNDLRNELYETFQEL
                     PFRFFDQAQSGQLMARATDDINNIRGMLMFALRAIVQAIGMLIIIAVIVLMTNFWLGV
                     VALSTTPLLIWWSIHFSITIRPVFLKIQQQFGVMTSALQENVAGGRVVRAFAQEKEEA
                     DRFEAELDELFERNLRATRQWSVSYPMTLALNALSVAGVIWVGGWMVLTGRLSFGTLV
                     QFERYTSMLQEPIRWLGFVVNRMARAIASGDRIFEILDTKRSIKDAPDATTMPDMRGV
                     VEFHDVSFGYAKGKADALHHVSFQARPGQITALVGPTGSGKSSVVSLIPRFYDVAEGQ
                     VTIDGRDVRSITLSSLRSQIGIVMQESFLFSMTVRENIAYGRPGASFEEVVAAAKAAR
                     AHDFILRMSEGYDTVIGERGVTLSGGQKQRLAIARALLVDPKVLILDDATASVDSETE
                     HDIQQALRTLMHGRTSFVIAQRLTTVRDADQVLVFENGEITQRGTHDELLTDSDGFYY
                     ELYSMQRQDQDDASRAVVNEGGPPPLEEERDRELGEPAISVPFTRAGGAS"
     gene            3041..4840
                     /locus_tag="VGR29_11990"
     CDS             3041..4840
                     /locus_tag="VGR29_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2074350.1"
                     /translation="MGQIRVQVDDEILGKAYDSRIASRLLGFLKPYRSRLLFTTAMVI
                     IGTIADLMLPKYFSMVVDEVRQEQRMSMVNLIGIAFVITVIIRFFANWGQYYTTQWLG
                     GRVVFDIRNRMFRHLQNLSIGYIDKRGVGTVMTRIQNDVAVIQELFSETVIGIISNVL
                     VLVGIIVIMLITNWKLALLSFLVLPIMTLVMTYWQKYAKAAYRMTRRTLSIVNANLAE
                     SIAGMRVVQAYTREPTNMKAFRKINGDNLDAAIEAAKYSSMLFPTVSFLSSASTAMIV
                     YFGGQLVFNEALSLGELVLFIAFIDRFFAPIRELSQQYSTMQSAMVAGERIFEVLDVE
                     PEVQDKPDAYELPEIEGKVDYNNVTFGYGNTEILHDIDLHVKPGETIAFVGETGAGKS
                     SMINLLMRFSDVWSGSITIDGHDIRDVTQESLRSQLGIVLQDTFLFGSTIRQNIAYAR
                     SHATSQEIEQAARDVGVHEFIEKLPDGYDTEVQERGVSLSIGQRQLISFARALLADPK
                     ILILDEATSSIDTQTEHLIQQALKRVLVGRTSFVIAHRLSTIREADKVVVMDEGRIVE
                     MGTHDELIAAKGMYYNLYTMQWRSQGTAAGSEE"
     gene            complement(5552..5983)
                     /locus_tag="VGR29_11995"
     CDS             complement(5552..5983)
                     /locus_tag="VGR29_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074351.1"
                     /translation="MRERTLYGLIVLATVMSLGHHIDHIIRGNHVGWPLLAEPTPFTY
                     SIGVYPLILLGLYLYGRGKVGPGYWALLSGAGVVFLSIIHFGPAAVEPPGDIINLYEP
                     RIIGWLAFAWLVAFIAVLVVTSIYEGHAWYRMRRGSAAGQR"
BASE COUNT         1237 a         1871 c         1873 g         1241 t
ORIGIN      
        1 ttggtccacc tcgggatcag cccccactgc atcggccgca gatgccatgc accgtcgtcc
       61 tcgacgatga cgggcagccg ctgggaaggc gcagcgttcc aggtcgggtt cgggtcgtaa
      121 tcgatctgga acgggacctg gcgcagtgtc tcactcagtt cctggaagtc ctcgatgaca
      181 tagcgtccgc acatgtgtga actccattcc tggttgatcc atatggcatc cacggccaat
      241 ccacaggtgg ttttaactat gccaccaccg gtctcgtgcg atgatccctc ccacatcgca
      301 cgagaccggt ggtggctgcg ggaactgatg atcggcggag ccagatctgt caggcggcgt
      361 cacggcggga cgagtctccc gtggacgggg ccttgccgag ggggagtggc tgcttgcccc
      421 cattgggccg gaaccgctcg atcatcggta cgggcgaaac gtgaagcacc cggtcctcac
      481 cggtgctgtg atcaacctgg gccgtactgc gtgccgagaa gaggagtggc gcaagggcca
      541 tggcagcgaa gagcaggagc atgagaacga caaggccatt aatcaggatt gcagtcatgt
      601 tcgacatcct ttccccggtg ttctgaaacc gggaccccgt agtcgggtga cgagacagat
      661 gtgtaaccag ttacgctccg gccgtaacca ccggtttcga gaagcgcttt catcggggaa
      721 aagggtcttc ctgatcctga ttcccggtac cggtggtacg acctgcgggg tggccgcact
      781 tctgtgtacg aacacatgtt ctcatacctg tgaccccttg tcaagcccgc acggacactt
      841 tttagaacac cggttctggc atcgcgggca ggctacgtgc aaaggtgata cggggcagga
      901 gaggcgcgag ggtttacaga tcaccggggt ggggtagact agttcgtcgg gagagtttgt
      961 accaggtgcg ccgcctctca cagggagccg ttagcgcgcc gaacctttgc acgtttcccc
     1021 cttcatgcac cggcgaatcc ggtgcacccg accgtttcgg gcctgggccc tgcggcacag
     1081 aatcatcgct ggccagctca ggctcggccc ggcaatgacc gcttggtcgt gcacgtccac
     1141 gaaccgggat cgatacccat ctccacggac ccagaggttg tctcgccgtg aaaaccctca
     1201 tacgaattct tggctatctg aagccataca gaacctggct catcactgtc tatgcggcac
     1261 tcttcatcgc gctcggtctg cagctgtcga tcccggcggt gctcggccgg gcaatcgacc
     1321 acggcgtcat cgcccgggat atgagctacc tcggcaaggc gaccctcgcg atcgttgggc
     1381 ttgcgacctt gcaggcgctc ttcacgttca ttcgcaccta cggcaccaat gttttggccg
     1441 agcgggtggg gaacgacctg cggaacgagc tctacgagac gttccaggag ctgccctttc
     1501 gctttttcga ccaggcgcag tccgggcagt tgatggcgcg cgctaccgat gacattaaca
     1561 acatccgcgg catgctcatg ttcgcgctgc gggcgattgt gcaggcaatc gggatgctca
     1621 ttatcatcgc ggtcatcgtg ctcatgacca acttctggct cggggtggtc gccctgtcga
     1681 cgacaccctt gctgatttgg tggtctattc acttttcgat caccatccgg ccagtcttcc
     1741 tgaagatcca gcagcagttc ggtgtcatga cctctgccct gcaggagaac gtcgccggcg
     1801 gacgtgtggt tcgcgccttc gcccaggaga aggaggaggc cgaccggttc gaggccgagc
     1861 tggacgagtt gtttgagcgg aacctgcgtg ccacgcggca atggtccgtg agctacccca
     1921 tgacccttgc cctcaatgcc ctcagcgtcg ccggcgtgat ctgggtcggc ggttggatgg
     1981 tcctgacggg gcgcctctcc ttcggtacgc tcgtgcagtt cgagcgctac acctcgatgc
     2041 tgcaggagcc catccgctgg ctcggcttcg tggtgaaccg catggcccgc gcgattgcct
     2101 ccggtgatcg tatcttcgag atcctggata cgaagcggtc catcaaggac gcaccggatg
     2161 ccaccaccat gcctgacatg cgtggcgtgg tggaattcca cgacgtcagc ttcggctatg
     2221 caaagggaaa agctgacgcc ttgcaccacg tctccttcca ggcacgcccg ggacagatca
     2281 cggcgctggt cggtccaacc gggtccggca agagctcggt cgtgagccta atccctcgct
     2341 tctacgacgt tgccgagggg caggtgacta ttgatggcag ggatgtccgc agtatcaccc
     2401 tctcatccct ccggtcgcag atcggcatcg tgatgcagga gagcttcctc ttctcgatga
     2461 cggtgcgcga gaacatcgcc tatggccgcc cgggtgcttc attcgaggaa gttgtggctg
     2521 cggcaaaggc cgcgagggct cacgacttca tcctgcggat gagcgagggc tacgacaccg
     2581 tcatcggcga gcgcggcgtc accctttccg gcggccagaa gcagcgccta gcgatcgctc
     2641 gcgccctgct ggtggatccg aaggtcctta tcctcgatga tgcgaccgcc agcgtggaca
     2701 gcgaaactga gcatgacatc cagcaggccc tgcggaccct gatgcatggc cgcacctcat
     2761 tcgtcatcgc ccagcggctg acgacggtac gggatgccga tcaggtcctc gtcttcgaga
     2821 atggcgagat cacccagcgc ggcacacacg acgagctgtt gaccgattcg gacggtttct
     2881 actacgagct ctactccatg caacggcagg atcaggatga tgccagccgg gcggtggtca
     2941 acgaaggcgg accgccccct ctcgaggagg agcgcgatcg ggaactgggg gagccggcga
     3001 tctctgtgcc gttcacccgc gccgggggag cgagctaacc atgggacaga tacgcgtaca
     3061 ggtagatgat gaaatcctgg gcaaggcgta tgacagccgg atcgccagcc gcctgctcgg
     3121 tttcctcaag ccctaccgct cacgattgct ctttaccacc gctatggtca tcatcggcac
     3181 gattgccgac ctgatgctgc ccaaatactt cagcatggtc gtcgacgagg ttcgccaaga
     3241 gcagcggatg tcgatggtca acctgatcgg catcgccttc gtgatcacgg tgatcatccg
     3301 cttcttcgcc aactggggtc agtactacac cacccagtgg ctcggcgggc gcgtggtctt
     3361 tgatattcgg aacaggatgt tccggcatct ccagaacctg agcatcgggt acatcgacaa
     3421 gcgtggcgtc ggcacggtca tgacgcgcat ccagaacgat gtcgcagtca tccaggagct
     3481 cttttccgag acggtgatcg gcatcatcag taatgtcctg gtgctggttg gcatcatcgt
     3541 tatcatgctg atcacgaact ggaagctggc gctgctcagc tttctcgtgt tgccgatcat
     3601 gacgctggtt atgacctatt ggcagaagta cgccaaggcg gcctaccgca tgacgcggcg
     3661 gacgctctcc atcgtcaatg cgaacttggc cgagagcatt gccggcatgc gcgtggtgca
     3721 ggcctacact cgcgaaccaa ccaacatgaa ggccttccgc aagatcaacg gcgacaatct
     3781 cgatgccgcg atcgaggccg ccaagtactc ctcgatgctc ttccccacgg tgagtttcct
     3841 gtcgtcggca tccaccgcga tgatcgtcta cttcggtggc cagctggtct tcaacgaggc
     3901 gctctctctc ggggagctgg tgctcttcat cgcctttatc gaccgcttct tcgcgccgat
     3961 ccgcgagctc agccaacaat acagcaccat gcagtcggca atggttgccg gcgagcgcat
     4021 cttcgaggtg cttgacgtcg agccggaggt ccaggacaag ccggacgcat acgagttgcc
     4081 cgagatcgag ggcaaggttg actacaataa tgtgaccttc gggtacggca acaccgagat
     4141 cctgcacgac atcgacctgc acgtgaagcc gggtgagacg atcgctttcg tgggcgagac
     4201 tggcgccggc aagagctcaa tgatcaatct gctgatgcgc ttctctgatg tctggagtgg
     4261 cagcatcacc attgacggac acgacattcg cgatgtcacc caggagtcgt tgcgctccca
     4321 gctcggtatc gtgctgcagg acaccttcct cttcgggagc accatccggc agaacatcgc
     4381 ctacgcccgc tcccatgcga ccagtcagga gatcgagcag gccgcccgcg atgtcggcgt
     4441 gcacgagttt atcgagaaat tgccagacgg gtacgacacc gaggtgcagg agcggggggt
     4501 cagtctctcc atcgggcagc ggcagttgat ctcctttgcc agggcgctgc tggcggaccc
     4561 gaaaatcctc atcctggacg aggccaccag ctcgatcgac acgcagaccg agcacctgat
     4621 ccagcaggcc ctgaagcggg tgcttgtggg ccggacatcg tttgtcatcg cgcaccggct
     4681 ctcgaccatt cgggaggccg acaaggtcgt cgtcatggac gagggccgaa tcgtcgagat
     4741 gggcacgcat gacgagctca tcgctgcgaa gggcatgtac tacaacctct acaccatgca
     4801 gtggcgtagc cagggcactg ccgccgggag cgaggagtaa ccgctaggcc aacgtgggat
     4861 caagcgtccc gtctggtggt gtaggtgacc gttcatggac aaactctccg cctttagtcg
     4921 gaggtgaaac tacgcagttt gggtgatgct gcgcttatgc aaagcaggga tactgtagag
     4981 gacccctccg gggacgagga ggagcatcga caacgaagac ggaaccatcc cccgtgtcgt
     5041 tggacaggct cccatagtcc gaccggacgc gacatcaatc gatctggtgg tgctgaatcc
     5101 tgatgcgcgc ggtccataga atttcgagga gaggcttcct cccggaagca cgatagggac
     5161 gacgacctgt tcccccctat catcgcctca ggtgaagacg ccggacgatg ggaacgtccg
     5221 gcattttcac gcctccgaca cggaatccgc acgtggttaa gcaagagcca ccaaggtggt
     5281 tggctggaag gtttcccggg atgtaaggac catggactga ttatccagat ctgcaagttc
     5341 ttcttaataa agcgttttgc ctgctcacta tattcataat actggcggtc aaaagacctc
     5401 ctacgatgac cataattgct gcactggact ggataagtaa agaatagagc cagtttggcc
     5461 cggtcattgg tgcagcgtcc cttacaatag aatctcctct tcggacggtg actctgccag
     5521 ataaaggggt tctaaatgcc ccagcccagc ctcaacgttg acccgctgca gatccccgtc
     5581 tcatgcgata ccatgcatgc ccctcataga tactagtgac gaccagcact gcgataaagg
     5641 ccacgagcca ggcaaacgcc agccagccga tgatccgtgg ttcgtagagg ttgatgatgt
     5701 cgcccggcgg ctcaacagca gcggggccaa aatgaatgat ggacaggaac accacccccg
     5761 ccccgctcag gagcgcccag tagccaggcc cgaccttgcc tctcccgtac aggtacagtc
     5821 ccagtaggat gagcgggtag acgccgatgc tgtaggtgaa cggcgtgggc tctgccagga
     5881 gcggccaccc gacgtggttg ccacgaatta tgtggtcgat gtggtggcct agggacatca
     5941 ctgttgcaag tacgatcagc ccgtaaagtg ttctttcccg catgctgctc ccgtcgctga
     6001 tagtgataga gccgtttttg gcgtgccagt cgacggctta gcgagatcct gtggaccgaa
     6061 acgctatccc agcacaatca gccaggagtg atcgccaccg aacgtcacag cctcactgtg
     6121 ctgcctgtgg tcgaatgcct cagggggaac tgttgggcgc gacggcctgg atgactgcct
     6181 gaacggcgtt gtagagcatg acaatgacgg ccacgaataa gc
//
LOCUS       DASYWM010000300         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_127589, whole genome shotgun sequence.
ACCESSION   DASYWM010000300 DASYWM010000000
VERSION     DASYWM010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127589"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..341
                     /locus_tag="VGR29_12000"
     CDS             120..341
                     /locus_tag="VGR29_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074352.1"
                     /translation="MELDSDPADRATDETATYSERVRERIRRLHATVNGDVTVEQALA
                     QWGEIVRVFGMEVAEQQLSAMERNAGIES"
     gene            complement(666..1040)
                     /locus_tag="VGR29_12005"
     CDS             complement(666..1040)
                     /locus_tag="VGR29_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2074353.1"
                     /translation="MDKRPIILVVDDEAPIRHVLLDLLADEGYQAMEAPNGRIALEMA
                     REHHPDLILMDVMMPELNGRQTLTALHAEPALATIPVVLMSAAGRAMAPADGVVTFLS
                     KPFDLQQVLDVVKTVLLNRSTP"
     gene            complement(1006..2568)
                     /locus_tag="VGR29_12010"
     CDS             complement(1006..2568)
                     /locus_tag="VGR29_12010"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEV2074354.1"
                     /translation="MSTHDGEHTAHPWTAQRVTTGVSGFDEVLDGGLLEGASCLISGG
                     PGTGKTTLGNQIAYHLAADDVNVLFVTVMAEAHDCMLMHIANFGFFRAELVGHRVHYV
                     SLVHQLAENGVSGAIQELRRMVHTYDAKLLVIDGIARCEDFAPSRAAYRSFVSELVAQ
                     MAAYRCSTLLLAQPDSESRTLHDIGTIVDDIVLLEDVSLGVHDTRLLRMLKMRGAVPL
                     RGQHEFVINHAGIDVYPRLEARPLAIQPARPMHQKRLPVGIDGLDSLLQGGLLTGSST
                     LIAGPPGIGKTTLGLHFIAEGAHRGTPGMIASFGEAPERLTSRATGFGLELERHVASG
                     AVRILWQPTSDLPLDAWANQLLAAVVEHRPQRLFIDGLTGLVRLASFPDRLPGFLSAL
                     GNALRAEGIATIISVETPTVEDAELDIPLPEAVATLDNVVLLRYVEPRSRLHRLISVL
                     RVRESGFDPGIREFTITDRGIEVASSSASAASVLGDDTNLPPSPPKSGEHDAAGGTSN
                     NDGQTTDHPGGG"
     gene            2914..>3266
                     /locus_tag="VGR29_12015"
     CDS             2914..>3266
                     /locus_tag="VGR29_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074355.1"
                     /translation="MATRRPPLSLSRRAALAGLAGGGLGQVLNPPASYAKARIADSAP
                     PRFEREPDIVYGENEREVQRLDIYRPVGWKSSRPAVFVFHGGGLVSGGRFQVSWPAME
                     FALAGYLAFAVGYRL"
BASE COUNT          630 a          962 c         1037 g          637 t
ORIGIN      
        1 gtgtatggag aaagcgacgg tgccaactgg aaggcactgc gcgacgagct gtttgagcga
       61 atgcgcctgc agaccgatct ccaagattcg cattagtcca tcgtggtctt ggagtcaatg
      121 tggaactgga tagcgatccc gcagaccggg cgacggacga gaccgccacc tacagtgaac
      181 gtgttcggga acggatccgt cgactgcatg cgacggtcaa tggagacgtc accgtcgagc
      241 aggcgttggc acagtggggt gagatcgtgc gcgtgttcgg gatggaggtc gcggaacagc
      301 aactgtcggc catggagcgc aatgcaggca tagagtcgtg agccggtaaa ctggcgaccc
      361 gagtggaccc tgccttttag ctcaatgcgt gcgagtcagc ggcttcctaa cgcatcaggg
      421 gccacgtgga tcgagggagc gagcacggta gggtgcggag aggagggatg cgtggttcct
      481 gcatggacga ttgagaggca accatgacca agcccctgac gcccgcgggg atctagggac
      541 tggcccttat ctatgcctag gagtgtcgtc ttccggcggg ccgggcgaag gagctgatca
      601 aggcgtacga gcgcttcgat cctttatgag atcttcgcca tcatctgcct gaggacacgc
      661 gcggattatg gcgtgctccg gttcaggagc accgtcttga ccacatcaag cacctgctgg
      721 aggtcaaagg gcttggacag gaaggtcacg accccatcgg ctggtgccat agcccggcct
      781 gctgcgctca tcaatacgac ggggatggtg gcgagggctg gctcggcgtg gagcgccgtg
      841 agggtctgtc ggccattgag ctcgggcatc atcacatcca tcaagatgag atcggggtgg
      901 tgctcccgtg ccatctccag ggcgatgcgt ccattggggg cttccatcgc ctggtatccc
      961 tcgtcggcaa gcagatccag cagcacatgg cgtatcggtg cttcgtcatc caccaccagg
     1021 atgatcggtc gtttgtccat cgttgttcga tgtgcctcca gcagcgtcgt gttcgccact
     1081 cttcggcgga gaggggggaa gattcgtgtc gtcaccaagc acgcttgccg cactcgcact
     1141 cgacgatgca acctcaatgc cacggtcggt aatcgtgaac tcccgaatcc cgggatcaaa
     1201 gccactctca cgcacccgca gcacggagat gagccggtgt aagcgcgatc gcggctccac
     1261 gtatcgcagc aacaccacat tgtcgagcgt tgccaccgcc tcgggcaggg ggatatcgag
     1321 ttcggcatcc tcgaccgtgg gggtctcgac cgagatgatc gttgctattc cctcagcccg
     1381 tagggcgttg cccagcgccg agagaaaccc ggggagccgg tcggggaagg atgccaggcg
     1441 gacgagtccc gtcaagccat caatgaacag tcgttgtggg cggtgctcga cgactgcggc
     1501 gaggagttga ttggcccagg catcgagcgg gaggtccgac gttggttgcc acaagatgcg
     1561 tacagcaccc gaagcgacat gccgttccag ctccagacca aaccctgtcg cacgggaagt
     1621 caggcgttcc ggtgcctcac caaagctggc gatcatcccg ggtgtgccac gatgtgcccc
     1681 ttcagcaatg aagtgaagtc ccagtgtggt cttgccgatc ccgggcggtc ctgcaatgag
     1741 cgtcgaggaa cccgtcagga gcccgccctg taggaggctg tccagtccat cgatgccaac
     1801 aggaagccgt ttctggtgca tgggtcgtgc tggctggatc gcaagcggcc gtgcctccag
     1861 gcggggatag acgtcaatgc cggcatggtt gataacgaac tcgtgttggc cccgcaacgg
     1921 tacggcaccg cgcattttca gcatgcgcag taaccgggtg tcatggacgc ccaagctgac
     1981 gtcctccaac aggacgatgt catcaacgat ggttccgatg tcatgcaggg tgcgactctc
     2041 gctgtcgggc tgagcgagca ggagcgtcga gcagcgatac gcagccatct gcgcgaccag
     2101 ttcggacaca aagctccgat aggccgcacg agagggtgcg aagtcctcgc agcgggcaat
     2161 cccgtcgatg accagcagtt tcgcgtcata cgtatggacc atgcgtcgca gttcctggat
     2221 cgcaccggat actccgttct ccgcgagctg atgcacgagg ctgacgtagt gaacccggtg
     2281 tcccacgagc tcagcacgga agaacccgaa attcgcaata tgcatgagca tgcaatcgtg
     2341 cgcttccgcc atgacggtca cgaagaggac gttcacatca tcagcggcaa ggtgataagc
     2401 gatctggttg cccagtgtgg tcttgcctgt tccggggcca cccgagatca gacacgaggc
     2461 gccctcgagc aggccgccat ctaggacctc atcgaatccc gagacccccg tggtgactcg
     2521 ttgggcagtc catggatgtg ctgtatgttc cccgtcgtgg gtcgacaccg attcttgtcc
     2581 ttatctctgc tcacggattg gcggaacgaa cgatctccaa tgtagcgcat gcacgagctg
     2641 tatggctgca cgccagcgat cttcacaaca caaggacaac tgccccccaa agggagctgg
     2701 agctacctcg tgtacttggg ttgcgtgtag gagatccccg tgacgtcgat gccggtatct
     2761 ggtgtggcgt ctttgcctgc catcgagatc ggatctgagg gtgcacgcca gtatcttttg
     2821 gccttaacga ggggtatgct gagaacagta cctccttggg aagccattga ccccgctgcc
     2881 cttgtgcctc ctcctcgaaa gaaggtgtcc tccatggcaa cccgccgacc acctctatcc
     2941 ctttcgcggc gggcggcgct agccggcctt gccggcggtg gactgggcca ggtccttaat
     3001 ccccctgcgt cgtacgccaa ggctcgaatt gccgactccg ctccacccag gttcgagcga
     3061 gagccggaca ttgtctatgg cgaaaacgag cgcgaggtcc agcgcctcga tatttaccga
     3121 ccggtaggct ggaaaagctc ccggccggct gtgttcgtct tccacggtgg agggctggtc
     3181 tccggcggcc ggtttcaagt gtcttggccc gcgatggaat tcgccctggc cgggtacctt
     3241 gccttcgccg ttggctaccg gctgtt
//
LOCUS       DASYWM010000301         5382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_540575, whole genome shotgun sequence.
ACCESSION   DASYWM010000301 DASYWM010000000
VERSION     DASYWM010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540575"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGR29_12020"
     CDS             complement(<1..364)
                     /locus_tag="VGR29_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074356.1"
                     /translation="MTESTVESFILEDDGTFPNNPLSVILYRQAIPLGEQDPARSTEE
                     RFVENGWSGLWRNGIFDFHHYHATAHEVLGCARGTVQVQLGGPGGQSVMLHAGDIVVL
                     SAGTAHRNLGHSDDYQIVG"
     gene            complement(473..1132)
                     /locus_tag="VGR29_12025"
     CDS             complement(473..1132)
                     /locus_tag="VGR29_12025"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEV2074357.1"
                     /translation="MKRFWIVAAAVATFVLVSFLLVEALGISRLVNPSNRIQDGGMGA
                     ALLGGGLLLADVLLPIPSSVIMIAHGAAFGIPRGTLLSLTTSVGGAMIGWYIGHRGRG
                     LLGRLVPPAEQENAALLIRRWGMTAIVISRFLPVVAETVAVMSRTTDLGWKRVLLAMT
                     IGTLPPALIYAIAGTLATDLASGVLVAVSVMMLAAIAWFVGRRLTAPASPAREPNPGQ
                     L"
     gene            1442..2002
                     /locus_tag="VGR29_12030"
     CDS             1442..2002
                     /locus_tag="VGR29_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2074358.1"
                     /translation="MTSVMRVHIVGGPGSGKTTLTRSLARECSLPLCELDTVAYEHGA
                     GAKRSLDLRLADVVAVSRQDTWITEGIFLGWTDELFDRADAIIWLDLPWHLAAWRILL
                     RHARASRVGANPHQGLRRLGPFLLDARRYYRAPANVAAAPDDDRAVTRDATANLLARY
                     RRKTIHCRSRQDVEAAMRSIAARCGG"
     gene            complement(2039..2713)
                     /locus_tag="VGR29_12035"
     CDS             complement(2039..2713)
                     /locus_tag="VGR29_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074359.1"
                     /translation="MSLPRISRRTFGTGLALSVPAVVAAHNATAQTPVATPEVASAPG
                     TDTFVVPVRPHDILAALLATPFDIGSTLLGMPFEPRRWTDIGQSPYISSVGGVLIQRS
                     DGAGDAGDQILGGYGVYLQPEGAQAARQLGELAHAEAAIETWPLVLGGLQGITIVHGI
                     GSPEVLTLVPVDNVLVIARDIDLGEGESDRGRTVIFRSVRHATVLLDHLERITSLQTW
                     SRQTPG"
     gene            2892..3641
                     /locus_tag="VGR29_12040"
     CDS             2892..3641
                     /locus_tag="VGR29_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEV2074360.1"
                     /translation="MEIVDREVALSEIDETVGPRPAERLVESVRRNGVIVPVILAEHV
                     DDDGELRLVIVDGNRRVAAARAAKLDAVPGRVLQQVSDPEMANLTLVTNSFRTSNYVT
                     EFWAMNELQRSGVNRQKLAEFTGMTPSTITTRELLAELDRRIFIGLSEGKIGPSIALA
                     VARLDPEIQRELGDRFARKGRLTKAEVDAVSPSRNDPPTELPEVDAEGRVLPDDLQYA
                     LVAIARQAVGRGIDEETWGEAAARAFREAQR"
     gene            3841..4110
                     /locus_tag="VGR29_12045"
     CDS             3841..4110
                     /locus_tag="VGR29_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074361.1"
                     /translation="MSTTSSGPNPVCEVGMKHPRDRHRMRPVDGAENVWFCSRHSMYA
                     QLVDPETAEAAKRGDEWGHYEGVDGLVVRQGDERQGGQIVYFREK"
     gene            complement(4387..4890)
                     /locus_tag="VGR29_12050"
     CDS             complement(4387..4890)
                     /locus_tag="VGR29_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074362.1"
                     /translation="MALHTRVPLRTRLLLAALLPGLATAMMVIPPLRSTWLSPMPCAN
                     CSKRQISPLPIFSRSGPDRSIFLFWLLVFVVSAPMVAFVVNWHWPAATNTNRAFLVAL
                     PQPVIVPLMLRFDVWLDLRGGYLLRDSGEEAMAYGIATVGGLLIGLILMILVAVGGQL
                     GARLGDR"
BASE COUNT         1049 a         1593 c         1781 g          959 t
ORIGIN      
        1 caccgacgat ctggtagtca tcgctgtgcc cgaggttccg gtgcgccgtt ccggccgaga
       61 gcacgacaat gtctccggca tgaagcatta cgctctggcc gccgggtccg ccaagctgaa
      121 cctggaccgt cccccgggcg cacccgagca cctcgtgggc ggtggcgtga taatggtgga
      181 agtcgaagat gccattgcgc cagagaccac tccatccgtt ctcgacgaag cgttcctcgg
      241 tagaccgggc cgggtcttgc tcgccaagcg ggattgcctg gcgatagagg atcacggaca
      301 gcgggttatt cggaaaggtg ccgtcatctt caaggatgaa tgattcgacg gtagattctg
      361 tcattgcggt acctccgtct cccaggaaag gcttgtctgc attgctgtcc cgcatcgcac
      421 tccatcgcac ggattgcacc tgtgggcacg cgaagcagct tgcggtccac gatcatagtt
      481 ggccgggatt tggttcccgt gccggagacg ccggtgctgt gagtcgcctc ccgacgaacc
      541 atgcgattgc cgccagcatc atgaccgaca cggcgacgag tactccactc gcgagatcgg
      601 tggccagggt gccggcgatc gcgtagatga gtgccggggg gagggtcccg atcgtcatcg
      661 caaggagcac cctcttccat ccgaggtccg ttgtgcggga catcaccgca accgtctccg
      721 cgacgacggg cagaaagcgg gaaataacaa tcgctgtcat tccccagcgg cggatcaaca
      781 gggcggcgtt ctcctgctcg gccggcggga cgaggcgccc cagcaacccc ctgccgcggt
      841 gcccgatgta ccagccgatc atggcaccgc cgacgctggt ggtcagtgag agcagggtcc
      901 cccgcgggat cccgaaggcc gccccgtggg cgatcatgat cacgctcgag gggatgggaa
      961 ggaggacatc agccagcagg agacctccgc ccagcagcgc ggcgcccatg ccgccatcct
     1021 gaatcctgtt ggacggattg acgagacggg agatcccgag ggcttcgaca aggagaaaac
     1081 tgacgagcac aaacgtcgcg acagcagctg cgacgatcca gaagcgtttc atcgtggggt
     1141 acatccgcgg aatgatatga gatcaagaga gcagatcatg cgcggctcca tgctgcaggg
     1201 gactgttgga tgggcttttg cttgctgctg ccaccggagc attgtatctg cttgtcgatt
     1261 gagtgcatcg cgggccagcg tttgcctatc ccggataggt ggaggagatt gacggtccgg
     1321 ggaagcgtcc atgcggaaag agatgttgct gctccgggag catcagcagg cttcaagccg
     1381 gcgggcggaa ggagcgagcc agcagcagtt aatagtgcca ttgaaacgaa gggatgtgtg
     1441 attgacatcc gtcatgcgcg ttcacatcgt cggtgggccg ggcagcggca agacgacgct
     1501 aacccgcagc ctcgcgaggg aatgctcgct cccgttatgc gaactggata ccgttgccta
     1561 cgagcacggg gcaggtgcga agcggtcact cgatctgcgc ctggccgatg tcgtagccgt
     1621 gtccaggcag gatacctgga tcaccgaggg catattcctg gggtggacgg acgagctgtt
     1681 cgaccgggcc gacgcaatta tctggcttga ccttccgtgg cacctggctg cgtggaggat
     1741 cctgctgaga cacgccaggg cgagccgcgt gggagcaaac ccgcatcagg ggttacgccg
     1801 gctgggccct ttcctgctgg atgcgaggcg gtactatcgt gcaccggcca acgttgcagc
     1861 cgctcccgac gatgaccggg cggtcaccag ggatgctacc gccaatctcc tcgcgcggta
     1921 taggcggaag accatccact gccggtcacg acaggacgtc gaggcagcaa tgaggtcgat
     1981 agcggcgcgt tgcggtggtt gatgctttcc aggacaccat gtctcttggt gggtcgcatc
     2041 aacccggtgt ctgacgagac caagtctgca gactcgtaat ccgctcaaga tgatccagga
     2101 gcacggtggc gtgccgcacg gaccggaaga tgaccgtgcg gccgcggtcg ctctctccct
     2161 ccccgaggtc gatgtcgcga gcgattacca ggacgttgtc caccgggacc agcgtgagca
     2221 cctccggaga tccgatgcca tggacgatgg tgataccctg gaggccaccg aggaccagcg
     2281 gccatgtctc gattgctgcc tcagcgtgag ccagctcccc gagctgcctg gccgcctgcg
     2341 ccccttccgg ttgcaggtag acgccatatc ctcccaaaat ctggtcaccg gcgtctcccg
     2401 cgccgtctga ccgctggatc aacacgccgc caaccgagga gatatatggt gactgaccga
     2461 tatcagtcca tctcctgggc tcgaagggca tacccaagag ggtagagccg atatcgaatg
     2521 gcgtcgcgag cagggcagca agaatgtcgt gtggacgcac ggggacgacg aatgtgtcag
     2581 tcccaggggc actggcgact tcaggtgtcg cgaccggtgt ctgcgcggtt gcgttatgag
     2641 cggccaccac cgccgggaca ctgagcgcga gcccggtgcc aaatgtacgg cgtgagattc
     2701 ggggcaagga catcgtcatg gcctccttta taaggaagaa cgatcaagtc ggttgctcgc
     2761 ggcgacatgg caccatcata tcagagcctc attcccgtgc agcgcacgga acgggtatag
     2821 aacatacgtt tcattcgtga taaggtcctg catatggctg agatagggct gcgtggactg
     2881 gaggggtgtc ggtggagatc gtggatcgcg aggtggcgct gtcggagatc gacgagacgg
     2941 tggggccgcg cccggcggag cgcctggtgg agagcgtgcg acggaatggc gtgatcgtgc
     3001 cggtgatcct ggcggagcat gtcgatgacg acggggagct ccggctggtg atcgtcgatg
     3061 ggaaccggcg cgtggcggct gcacgggcag ccaagctcga tgcggtgcca gggcgtgtcc
     3121 tccagcaggt ttccgatccg gagatggcca acctcacatt ggtcaccaac agcttccgga
     3181 ccagtaacta tgtcaccgag ttctgggcga tgaacgagct ccagcggtcg ggagtcaatc
     3241 gccaaaagct ggccgagttc accggcatga ctccctctac catcaccacc cgcgagctcc
     3301 tcgcggagct ggaccgccgc atcttcatcg gtctcagcga agggaagatc ggtcccagca
     3361 ttgcgctggc cgttgcgcgg ctcgacccgg agatccagcg ggagcttggc gatcgctttg
     3421 cgcgtaaggg gcgcctgacg aaagccgagg tcgacgcggt ttcgccgtcg cggaatgacc
     3481 ctcccaccga gctgccggag gtggatgccg aaggccgggt gctaccggac gacctgcagt
     3541 atgccctcgt cgccattgcg cgccaggcgg ttggccgggg catcgacgag gagacctggg
     3601 gagaggcggc ggcacgtgcg tttcgcgagg cgcagcgcta gcagccgggc ggaagcgacg
     3661 cgccccggga acgagaccat tcctttatag atagatgggg caacccacgc ggagacgaca
     3721 tcggaggggc gctcgggatt gcctgcgttc cggcgtgccc gtccccgacc tgccgttcgg
     3781 ttctatactc agggtgagat gtgcagatcg ttccattcac cagggagtac cgttttacct
     3841 atgtccacaa catcatctgg cccgaatccc gtctgcgaag tggggatgaa gcatccacgc
     3901 gaccggcacc ggatgcgccc ggtcgacggc gcggagaatg tgtggttttg cagccggcat
     3961 tcgatgtacg cgcagctggt cgacccggaa accgcagagg cggccaagcg tggcgacgaa
     4021 tggggtcact atgagggggt cgacggcctc gttgtccggc agggtgacga gcgccagggt
     4081 gggcaaatcg tctacttccg ggagaagtaa caggttccct ctaacactct tgaaatggtg
     4141 cggcattcca agggcttttc attccgttct gacacaccgg tcaggattgc cgcgcatttc
     4201 ccgtgaatgg tgtcgtgtct ggaacctccg ccaagagctg gacgatcgcc cgggcccaca
     4261 cggcttccac gggaatgaca acgacggatt cggctgccag tcataccaag aggtcattga
     4321 cgcggctccc gatccttggg ggccgttgtc gtgcctggtt gggtgcacga tctccgttgc
     4381 cagagcctac cggtccccga gtcgggcccc cagctgtccg ccaacggcca ccaggatcat
     4441 gaggatgagc ccgatgagga gaccgcccac ggttgcgatc ccgtaggcca tcgcctcttc
     4501 gcccgagtct cgcaacagat atccgccccg aaggtccagc cagacatcga aacggagcat
     4561 cagcggcacg atgacaggct ggggcagggc aaccaggaat gcccggtttg tgtttgtggc
     4621 ggcgggccag tgccagttca cgacgaatgc gaccatcggg gcgctgacca caaacacgag
     4681 caaccagaac aagaagatgg atcgatctgg ccccgacctg ctgaagatcg gcaggggaga
     4741 gatttgcctc ttggagcagt tcgcgcatgg catgggcgag agccaggttg agcgcagtgg
     4801 agggatcacc atcatggcgg ttgccagtcc gggcaagagg gcggcaagca ggagtcgggt
     4861 tcggagaggg acacgtgtgt gaagagccac tctgttccgc ctttccgtgc gccggtgagc
     4921 cacctcgatt gtcagacaat gatccccctc atcatatatc ggacacgtgt ggtggctatc
     4981 accggaaaca gacggagagg gagatgaacg acgcagctcc tgagacctcg tgggcattgt
     5041 cgtgctcgga aagccacgac atatgcattc ccggcattcg agccgactgt ccctgcgcgc
     5101 accatccgta ggacgccgaa tacgtatttg tacagaggtc ggattgttat cgctgcacta
     5161 gagtacaaac agaggctgcc gcttggccca cacaacggag gcagcacgct ctcttcccaa
     5221 tcggtgtacg tcgtcagcta gccatccctt gaggatgggg gaggtcacca tgacagtcgc
     5281 ctatttttca gacgtcccga acggcacgcg tgaacaggcc cagcaggtcg gtgcgttcgt
     5341 ccaggagcag ctcggcggac cggacgtagc cccggacggt gg
//
LOCUS       DASYWM010000302         4590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_52543, whole genome shotgun sequence.
ACCESSION   DASYWM010000302 DASYWM010000000
VERSION     DASYWM010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52543"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..1101)
                     /locus_tag="VGR29_12055"
     CDS             complement(283..1101)
                     /locus_tag="VGR29_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074363.1"
                     /translation="MEEQEEGSRKSCFVIAPLGEDGSVVRVRSDKVFRHVIKPAVEEC
                     GYSPLRSDIEPRPGMIGQQIISHLLQDDLVIADLTGLNANVFYELAIRHMVSKPVVQI
                     IQKGESLPFDITQSRTIFLDHTDLDSVERCRQEIITQIGELERDPSLVENPVSQAIRI
                     ETLSQSPDPKDRHDAKFLARLDRLSAQVEMLLLDRQCQRPEIGGSLANGPVVGRPADA
                     VPGSSNANQPGPTPGSPFDRVGGMSVTLEQAVQALGHPQGAGDHPGTCGDSDPA"
     gene            1299..2063
                     /locus_tag="VGR29_12060"
     CDS             1299..2063
                     /locus_tag="VGR29_12060"
                     /inference="COORDINATES: protein motif:HMM:NF009389.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2074364.1"
                     /translation="MELSLAGRIALITGASRLAGIGAATARMFAAAGADIAFTHWTPY
                     DQASYGTTSSEPETLVNELRDMGVRAEGMEVDLSDASSPARLFDTIEARLGPVSILVN
                     NAAYSTHDDHETLTAESLDRHYAVNVRAMALLSVEFARRFTAGSGGRIINLTSGQSVA
                     PMPAELSYATTKGAIEAFTTSLAPGVAKKGIKVNAVDPGGTDTGWMTDEVKAAITSEM
                     AFGRVGLPEDAARLILFIASDAGRWITGQVIHSRGA"
     gene            complement(2083..3162)
                     /locus_tag="VGR29_12065"
     CDS             complement(2083..3162)
                     /locus_tag="VGR29_12065"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:TIGR02089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEV2074365.1"
                     /translation="MESRTIRIAVIPGDGIGKEVVPEGLRVLNRLAERSGGRLSFEFE
                     SFPWGCEHYLATGRMMDVDGLERLAPFDAIYFGAVGWPDVPDHISLWGLRLAICQGFD
                     QYACIRPVQLLPGITSPLRNASPESMDWVVVRENSEGEYAGIGGRNLASRGEASEVAL
                     QTALFTEKGCERIIRYAFNLARSRSRPKVTSVTKSNAQQYAMTLWDEVFARVAKDYPD
                     VETEQFLVDAMAARFVLRPETLEVVVGSNLFGDILSDLGSALAGSLGIAASANIDPER
                     RHPSMFEPVHGSAPDIAGQGIANPIAAIWSGALMLDHLELPEQAAAVVRAIKATTAAG
                     VLTPDLGGTATTREVTDAVLSHLDG"
     gene            3525..4190
                     /gene="nth"
                     /locus_tag="VGR29_12070"
     CDS             3525..4190
                     /gene="nth"
                     /locus_tag="VGR29_12070"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV2074366.1"
                     /translation="MEKRSFDIDIAMQRIREAVAPFPKAAMFELRDDGYGSPFEQLIA
                     CMLSIRTRDEVSVVASRRLFARARTPGALAMMTPDEIDALILDVTFHERKVVQIRDIA
                     IRARDEFAGELPCDPELMQSFSGVGPKCANLALGIACNQQRISVDIHVHRVTNRWGYV
                     HTRTPEQTMTALEGVLPGRYWIEINERLVPFGKHICTGVRPKCSTCPVLEMCQQVGVT
                     SHR"
     gene            complement(4187..>4590)
                     /locus_tag="VGR29_12075"
     CDS             complement(4187..>4590)
                     /locus_tag="VGR29_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2074367.1"
                     /translation="VMEDGHHFDLGGRGLEVLHCPGHSPGGIALLDRANWALFSTDVA
                     YAGALYVYDPANLPTYQASLQRLADLVPILSAVYPAHDASPIKPALLLGMAQGVRDAM
                     DGMEPSSRAGDMARYEFDGFALQVWGMPEPA"
BASE COUNT          904 a         1488 c         1352 g          846 t
ORIGIN      
        1 cccccccccc ccccccaccc cctctcttcg ctcgcagata ttactgacag tcctgtgcag
       61 cggcgtcttg agcccgtcct gcctcccaca ccacgggtca gccccaaaca agacccatcc
      121 cattgcggcc gtgtcacgag cagggagcgc agttaaactg attggctcgt acagattcct
      181 cgcacacgat cgtcattcag acccttagcg acgtcgaagg ggatggatct tacgcgaagc
      241 gctgtcgact gccagagacg gccgaggagg attcgcttgg cactacgccg gatccgaatc
      301 gccacacgtc ccgggatggt cgccggcacc ctgcggatga ccgagggcct gaaccgcctg
      361 ctcaagcgtt accgacattc ctccgacgcg atcaaaaggc gatcccggag ttggaccagg
      421 ctgattcgcg ttgctgctgc ccggcaccgc gtcagcagga cgcccgacga ctggcccgtt
      481 cgcaagagac ccaccgatct ccggacgctg gcactgccgg tccaaaagga gcatctcgac
      541 ctgggcgctc aatcgatcca gtcgcgcgag gaacttcgcg tcatgtcgat cctttggatc
      601 ggggctctgg ctcaacgttt cgatcctgat cgcctgcgaa accggattct cgacgagcga
      661 cggatctcgc tccagctccc cgatctgcgt gatgatctcc tgccggcatc gctcgacact
      721 gtccaggtcc gtgtgatcga gaaagattgt gcgactctgg gtgatgtcaa aaggaaggga
      781 ttcgcccttc tggatgatct ggaccaccgg tttgctcacc atatggcgga tggccagctc
      841 atagaagaca ttggcgttga ggccggtcag gtctgctatc accaaatcat cctggaggag
      901 atggctgata atctgctgcc cgatcattcc cggtctcggc tcgatgtcgg atcgcagagg
      961 ggaataccca cactcctcta cagccggctt gatgacatgc cgaaacacct tgtcggaacg
     1021 aacgcgaacg acactgccgt cctcaccgag cggggcaatg acaaaacacg attttctcga
     1081 tccctcttcc tgctcttcca ccttggtgcc ccttgttcac gtgttcgtgc aggttcgaca
     1141 tcgcggtatg atgcagccgc gcctgaggta ccgcaggcgc aagagagata ccacactcac
     1201 cgactggttg atccagaagg acgaaccggg ggaacgcaac tatcacagcc atttcaggtg
     1261 catcaccgga acatggagaa gcacacgcgg aggaactcat ggaacttagc ctcgcaggac
     1321 ggatcgccct gatcaccggc gccagccgcc tcgccggcat tggcgcggcg actgcccgga
     1381 tgtttgcagc cgccggagcg gatatcgcct ttactcactg gaccccatac gatcaagcat
     1441 cctatggcac tacctcctcc gagccggaga cactggtcaa cgagcttcgc gacatgggtg
     1501 tccgcgcaga gggtatggag gttgatctgt cggatgcttc ctcccctgcc cggctcttcg
     1561 ataccataga agcgcgcctg ggaccggtga gcatcctcgt gaacaacgcg gcgtactcca
     1621 cgcacgatga ccacgagacc ctgacagcag agtcactgga tcgtcactac gcggtgaacg
     1681 tccgggcgat ggcgctgctc tccgtggagt tcgcgcgccg cttcaccgcc ggcagtggcg
     1741 gacgcatcat caatctgacc tccgggcaaa gcgttgcccc gatgcccgcc gagctttcct
     1801 acgccaccac aaaaggtgca atcgaggcct tcacgacttc ccttgccccc ggagtcgcga
     1861 agaagggaat caaagtcaat gccgttgacc caggcgggac cgacaccgga tggatgacag
     1921 acgaggtcaa ggccgcgatt acgtcggaga tggcttttgg ccgcgttgga ctgccggagg
     1981 atgccgcccg cctgatcctc ttcattgcga gcgatgctgg ccgctggatc accgggcagg
     2041 tcatccactc gcgtggtgcc tgaccaactg tggaaaaggc cgtcacccgt ccagatgcga
     2101 cagcacggcg tccgtgacct ctcgggtggt ggctgttccg ccgaggtcag gcgttagcac
     2161 accggcggca gtcgttgcct tgatcgcgcg gacgactgcc gccgcttgct cgggcaactc
     2221 gagatggtcg agcatcaggg caccgctcca gatcgccgca atcgggttgg cgatgccctg
     2281 acccgcgata tctggcgcgg atccgtggac cggctcgaac atgctaggat gtcgtcgctc
     2341 gggatcgatg ttcgcgctcg ccgcaatacc gagacttccc gctagggcgc ttcccaggtc
     2401 tgacaggatg tcgccgaaga ggttggagcc gacgacgacc tcaagcgtct caggccgaag
     2461 cacaaaacgc gctgccatcg cgtccaccag gaactgctcg gtttcgacat ccgggtagtc
     2521 tttggcgaca cgagcgaaga cctcgtccca aagcgtcatc gcatattgct gagcattgct
     2581 ctttgtcacg ctggtcacct tgggacgcga ccgcgagcgt gcgaggttga aggcatagcg
     2641 aataatgcgc tcgcatcctt tctcagtgaa gagagcggtc tggagggcaa cctcggacgc
     2701 ttcaccacga ctcgccaggt tacgtccgcc gatgccggcg tactcgccct cggagttctc
     2761 ccggactacg acccagtcca tcgactcggg agaggcgttg cgcaatgggc tggtgatccc
     2821 aggcagcaat tggaccggac gaatacaggc gtactggtcg aagccttggc agatggcgag
     2881 acgaagaccc cagaggctga tgtggtccgg cacgtcgggc cagccaacgg ccccgaagta
     2941 gatcgcatca aacggcgcga gacgttcaag gccatcgaca tccatcatcc tgccagtcgc
     3001 cagatagtgc tcgcaccccc atggaaacga ctcgaactcg aaactcaggc gtccaccaga
     3061 gcgctcggcg agccggttaa gtacccggag accttcaggg accacttctt tcccgatccc
     3121 gtcgcccggg atgacagcga tgcgaatagt cctcgactcc atgcggttcc cctcccctga
     3181 ctctgcgatg tcaagtactg cccttgatgg tgacgttggt tggattgcac accggttgcc
     3241 acactcagga gtgtcacatc aaagcgtgcc gcgaggacct cacggcgagc ctcgtcctgt
     3301 tgaccgcgaa ggggcccgtc agccgaacac tacacctatc gcccactccg gtcgttcgtg
     3361 ggacagccga cattcgagcc tcgctcatat caggtatcgc tccgggagtg gcatgacgat
     3421 ggtcctcgac gttgcctgac gatcatgcag gatggagtcc tgcctttcgc cccgggctcc
     3481 agcggcacac ggcgtgcacg tgatggggta attggaggac aacgatggag aagcgctcgt
     3541 tcgacatcga catagccatg caacgcatcc gggaggcggt ggcacccttc cccaaggctg
     3601 ccatgttcga gctgcgggat gatggctacg ggtcaccctt cgagcaactc atcgcctgca
     3661 tgctctcgat ccgcacgaga gacgaggtta gtgtcgtcgc ctcccgccgc ctctttgcga
     3721 gggcgcggac gccaggcgca ctcgcgatga tgaccccgga cgagatcgat gcgctcatcc
     3781 tcgacgtgac cttccacgag cggaaggtgg tccagattcg cgacatcgcg atccgcgccc
     3841 gcgacgagtt cgcgggcgag ctcccctgcg atcccgagct gatgcagtcg ttctccggtg
     3901 tcgggccaaa atgtgcgaac ctcgcgctcg ggatcgcctg caaccagcag cggatcagtg
     3961 tcgatatcca tgtccaccgg gtcactaacc gctgggggta cgtccacacc aggacccctg
     4021 aacagacgat gaccgccctg gagggagtgc tgcccggacg gtactggatc gagatcaacg
     4081 agcggcttgt tccttttgga aagcatatct gcaccggcgt ccgccccaag tgctcaacct
     4141 gccccgtact cgagatgtgc cagcaggttg gggtcactag ccaccgctag gccggttcag
     4201 gcatccccca cacctgaagc gcgaaaccat cgaactcgta gcgcgccatg tcgcccgcgc
     4261 ggctggacgg ctccatacca tccatagcgt ccctgacccc ctgcgccatg ccaagaagca
     4321 gcgctggctt gatcgggctg gcgtcatgcg ccgggtagac ggcactcaat attggtacca
     4381 agtccgcgag acgttgcagt gaggcctggt acgttggcag gttggctgga tcatagacgt
     4441 agagggctcc cgcgtacgcg acatcggtgc tgaatagtgc ccagttcgcc ctgtccagaa
     4501 gagcaattcc gccgggggag tggccagggc aatgcagcac ctcaagcccg cgccctccaa
     4561 ggtcgaagtg atgcccgtcc tccataacgc
//
LOCUS       DASYWM010000303         5122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_16132, whole genome shotgun sequence.
ACCESSION   DASYWM010000303 DASYWM010000000
VERSION     DASYWM010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16132"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VGR29_12080"
     CDS             <1..636
                     /locus_tag="VGR29_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074368.1"
                     /translation="RQHSRHFRVDAMNEIERPWNAMPPRAVANLFRAYPGRWWIAGGW
                     SLDLYLGHPTRLHEDTDVLVLQRDLEHVHALLPGWVIMAADPPGTLRHWIEGETLPEG
                     TRDIWCRPSTSDVWRFQLMVICTEGDRWIFPRDSRLTGEIAQLSDVREGIPIIAPEFQ
                     LLYKSRVPHRSRDASDLRRMIPRLSRRRQAWLRERVELLYPDSPALELLQL"
     gene            783..1559
                     /locus_tag="VGR29_12085"
     CDS             783..1559
                     /locus_tag="VGR29_12085"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2074369.1"
                     /translation="MFRLDGKTALVTGGASGIGREIALLFGQQGATVAIGDINEEAAT
                     SVAEEVTDAGGAAFGVRLDVTDMVSAESATASIVDRAGSLDILVNNAGIGMVGNLTET
                     SPEDFARLIAVNVYGVYNCSHAGVKQMLSQDPKGGCIINMASIAGQVAVARRFAYGTT
                     KGAVISMTQSTAMDYVDQGIRCNCICPGTVETPFVEAYLQRYHAGEIEETRKQLHARQ
                     PLGRMGRPQEIAPLALYLASDEAAYVTGAQMVIDGGLTAR"
     gene            1583..3172
                     /locus_tag="VGR29_12090"
     CDS             1583..3172
                     /locus_tag="VGR29_12090"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEV2074370.1"
                     /translation="MKSVAPRKPLLGTANRGRVDFADVAVLLNNEDDVAIAKNALLPR
                     TVLQREGQELVIAQMIPPGHKFAIHAIPKGGDIRRYGQIIGFASTDITAGQHVHTQNV
                     EVHQGELDLDYAFCADYRPVDMVPEAERRTFMGYRRADGRVGTRNYVAVLASVNCSST
                     ATRRVAEYFRDNPIMDQFPNVDGVVGFPTKGGCGAHYGSDYLGLLQRTMAGIIDHPNI
                     GGYIILSLGCETNQPEDLIANTSLNEDAPLVLTIQQDGGFRQSVRAGIEAVINLLPEA
                     NKIEREPIPASEIVLALQCGGSDGWSGVTANPALGRAADEIVRQGGTVVLGETSEVYG
                     GEHLLTRRAVSPEVGQKLVDKIHWWEDYTSMFGAEIDNNPSPGNKLGGLTTIYEKSLG
                     AIAKAGTTPMTDVVGYAERITSRGFVHMDTPGYDPVSVTGQVAGGCNIVCFTTGRGSA
                     FGFKPAPSIKIATNSDLYAKQEPDMDINAGRMLDGITLDELSMEIFEQILDTASGEKT
                     KSELDNIGEEEFNPWIIGATL"
     gene            3148..3708
                     /locus_tag="VGR29_12095"
     CDS             3148..3708
                     /locus_tag="VGR29_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HEV2074371.1"
                     /translation="MDHRRDTLTPLFATFSTNLAAAGGIAHHAQSTTEAVETIAGLPG
                     DLHPKVIWVSSRVQECQPEIIAGLQAAGYELAVPDRPATVRDEPVGLTVARVGIAETG
                     SVLLDEPEIADRSVSLMTNLLIVVCPVDRLVPSLDDAAGVLREISHSGSSYATFVTGP
                     SRTADIERQLTIGVQGPAVMHVVFVP"
     gene            3915..>5122
                     /locus_tag="VGR29_12100"
     CDS             3915..>5122
                     /locus_tag="VGR29_12100"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEV2074372.1"
                     /translation="MTTDTSHGHPETPFEERYQHAVEDLQLSRNLTNFQQSWKQSRGA
                     VMEDIDFQALRKQMKAAKSEAIDNLDHYLDQFIESAKRAGTTVHLADDADQATRIVRD
                     LARQRSVKLVAKSKSMVSEEIELNEVLLKDGIEVVETDLGEWIVQLRGERPSHMVMPA
                     IHLSRQQVGDVFSERLGREISRGDVEEQVRAARDAIRDVFFNAGMGVTGANALIAESG
                     TVMMVTNEGNGRLTSSIPPVHVVLAGIEKLVPTYEDAMTQLRLLARSATAQRLTVYST
                     FIQGPSPGHEQHIVLVDNGRRTMRDLPEFREALHCIRCAACANVCPPYREVGGHVFGY
                     IYSGAIGLVVTPFMHGIDAAAGPQSLCLSCNACEVVCPVEIPLPRQILDVRAMVVKQK
                     GMKRSKQVILS"
BASE COUNT         1014 a         1580 c         1527 g         1001 t
ORIGIN      
        1 cgccaacatt ctcgtcattt cagggtagat gccatgaatg aaatcgagag accgtggaac
       61 gccatgccgc cgagggcggt ggcaaacctc ttccgggcgt atccaggtcg gtggtggatc
      121 gcaggcgggt ggtcgctgga cctttatctt ggacacccca cccggctcca cgaggatacc
      181 gatgtgctgg tcctgcaacg cgatttggag cacgtccatg cattgcttcc tggctgggtc
      241 attatggcgg ctgaccctcc gggcacgcta cggcactgga tcgaggggga gacgctcccg
      301 gaggggaccc gggacatatg gtgtcgtccg tcaacatcgg atgtgtggcg gtttcagttg
      361 atggtcatct gcactgaggg cgatcgatgg atattccctc gtgacagccg gcttactggt
      421 gagatcgccc agctctcgga tgtacgtgag ggtatcccga tcatcgcacc ggagttccag
      481 ctcctctaca agtcacgcgt tccgcatcgt tccagggacg ccagtgacct tcggcggatg
      541 atccctcggc tctcacgaag gcgacaggca tggctccgcg agcgagtcga gcttctctat
      601 cctgacagtc ccgcgctgga attgcttcaa ctttgatcca tccctcggcg cacgattccc
      661 cggccgtctt tagccttgat ttcgctatgc agcacctgcg attttgtata caatcggcat
      721 gttactcggt gtttgccgac gcaattcgta cctttgaatc gctcacgatc cggggagagt
      781 ggatgttcag gctcgacgga aagaccgcac tcgtgaccgg aggcgcctcg ggaatcggac
      841 gggagattgc cctgctcttt ggtcaacaag gggcgacggt tgcgatcggg gacatcaacg
      901 aggaggccgc gacatcggtt gctgaagagg ttaccgacgc aggtggtgca gcgttcggtg
      961 ttcggcttga tgtcactgac atggtctccg ccgaatcggc cacagcatcg atcgtggatc
     1021 gcgccggctc cctggatatt cttgtgaaca atgctgggat tggcatggtg ggcaatctca
     1081 cggaaacgtc tccggaggac tttgcccgcc tgatcgccgt caatgtctat ggtgtttaca
     1141 actgctccca cgctggtgtg aagcagatgc tctcccagga cccaaagggc gggtgcatca
     1201 tcaatatggc atcgatcgcc ggacaggttg ccgttgcccg ccgcttcgct tacggcacca
     1261 ccaagggcgc agtgatctcc atgacgcaat ccacagcgat ggattacgtc gatcagggca
     1321 ttcgttgcaa ctgtatctgt cctggcaccg tcgagacgcc tttcgtggag gcctacctgc
     1381 agcggtacca tgcaggcgaa atcgaggaga cacgaaagca gctccatgct cgccagccac
     1441 ttggccgtat gggccgtccg caagagattg cgccgctcgc actctatctc gcctcagacg
     1501 aggccgctta tgttaccggc gcccagatgg ttatcgatgg cgggctcacc gcccgctagc
     1561 cgaagtacgg aaggattccc tcatgaaatc ggtcgcaccc cgcaaacccc ttcttggtac
     1621 cgccaatcgt ggtcgtgttg acttcgctga tgtcgcggtt ctgctcaaca acgaggatga
     1681 cgtcgcgatc gcgaaaaacg cccttctccc ccgcaccgtg ctccagcgcg aggggcaaga
     1741 actcgtcatc gcgcagatga tcccgcccgg acataagttt gccatccacg ccattccaaa
     1801 gggaggtgac atccgccgct acggccagat catcgggttt gcgtccaccg atatcacagc
     1861 agggcagcat gttcacaccc agaatgtcga ggtgcatcag ggcgaactcg acctggatta
     1921 cgccttttgt gctgactatc gtccagtcga tatggtgcca gaagcagagc gtcgcacctt
     1981 catgggatac cgccgtgcgg atggtcgcgt gggcacccgc aactacgtcg ccgttctcgc
     2041 gagcgtgaat tgctcgtcaa cagcaaccag gcgcgtggcg gaatacttcc gtgataatcc
     2101 gatcatggac cagttcccga acgtcgatgg cgtcgtcggc ttcccgacca agggcggatg
     2161 cggcgcgcac tacggctctg attatctcgg tctcctccag cggaccatgg ccggcattat
     2221 cgatcatccg aacatcggcg gctacatcat cctgagcctc ggctgtgaga ccaaccagcc
     2281 ggaagacctg attgccaaca cctcactgaa tgaagacgcg ccacttgtgc tcacgatcca
     2341 gcaggatggc ggcttccgcc aatctgtccg cgccggtatc gaggccgtga tcaacctcct
     2401 gccggaggcc aacaagatag agcgggagcc gatcccggcc tccgagattg tcctggcact
     2461 ccagtgcggt ggctcggatg gctggtcagg cgtgacagca aacccagcgc tcggccgcgc
     2521 cgccgacgag atcgtccgcc agggaggaac cgtagtcctg ggagagacaa gcgaggtata
     2581 cggaggtgag cacctcctga cgcggcgagc cgtctctccg gaggttggcc agaaactggt
     2641 tgacaagatc cactggtggg aggactacac gtcgatgttc ggggctgaga tcgacaacaa
     2701 tccctcgccc ggcaacaaac tcggtggcct gaccacgatc tatgagaagt ccctcggcgc
     2761 aattgcgaag gccgggacca ccccaatgac ggatgtcgtc ggctacgccg agcgcatcac
     2821 cagccgcggc ttcgtgcata tggacacccc gggatacgac cccgtgtccg tgacggggca
     2881 ggtagccggt ggctgcaaca tcgtctgctt caccaccggt cgtggttccg ccttcggctt
     2941 caaacctgca ccctcgataa agatcgctac caactccgat ctctacgcaa agcaggagcc
     3001 ggacatggac atcaacgccg gccggatgct cgatggcatc acgctggacg agctgagcat
     3061 ggagattttc gagcagattc tggatacggc ttccggcgag aagaccaaga gcgagctgga
     3121 caacatcggc gaggaagagt tcaatccatg gatcatcggc gcgacactct gacgccgctc
     3181 tttgcgacgt tctcgacaaa ccttgccgcg gctggcggaa tcgcgcacca cgctcagtcc
     3241 acgacggaag cagtcgaaac aatcgcgggc ctgccgggtg accttcaccc caaggtgatc
     3301 tgggtatcgt cccgcgtgca ggagtgtcag ccagagatta ttgcggggct tcaggccgcc
     3361 ggatacgagc ttgcggtgcc agacagacca gcgacggtgc gggacgagcc tgtagggctg
     3421 accgtcgccc gagtcgggat cgctgagacc ggatcggtgt tgctcgatga acccgagatc
     3481 gcagatcgat ccgtgagcct aatgacgaac ctgctcatcg tggtttgccc cgtcgatagg
     3541 ctggtaccgt cgcttgacga tgcggctgga gtgctccgcg agatttccca ctccggctca
     3601 tcctacgcca ccttcgtcac cggccccagc cgcacggcag acatcgagcg ccaacttacc
     3661 attggtgttc agggccctgc cgtgatgcac gttgtgttcg ttccgtaaac gacgtgacct
     3721 tgagggacaa cagcgtgaat tgaggggcca acctgtgagt ctcgtgacca attcgcttcg
     3781 cgtcggattc ttcgctccgc tcagaatgac gatccgttgt agccattagc tccgtagagt
     3841 ggaatccgcc cacgcgagat cccgctgtac tgtgtgctgt atgacaatga ccatcctccg
     3901 aaaggccgcc ttgcatgacg accgatacct cccacgggca ccctgagacc ccattcgaag
     3961 agcgctacca gcatgccgtc gaggatctgc agctctcccg caacctgacc aacttccagc
     4021 agtcatggaa gcagtcgcgc ggggccgtca tggaggatat cgacttccag gcgctgcgga
     4081 agcagatgaa ggccgccaag agcgaggcca tcgataacct cgatcattac ctggatcaat
     4141 tcatcgagtc agcgaagcgc gcaggtacca ctgtgcacct ggccgatgat gctgaccaag
     4201 cgacccgaat cgtacgcgac ctggcgcgac aacgatccgt gaaacttgtt gccaaatcga
     4261 agtcgatggt cagcgaggag atcgagctca acgaggtgct cctcaaggac gggatcgagg
     4321 tcgtcgagac ggatctcggg gaatggatcg tccagctccg gggcgagcgg cccagtcaca
     4381 tggtgatgcc cgcgattcac ctctcgcgcc aacaggtcgg cgatgtcttc agcgaacgcc
     4441 tcggccggga gatttcccgt ggcgacgttg aggagcaggt tcgcgcggcg cgggatgcca
     4501 tccgcgatgt cttcttcaac gcgggcatgg gggtcacggg cgccaatgca ttaatcgcag
     4561 aatccggcac ggtgatgatg gtcacaaatg aggggaatgg ccgcctcacg tcctccatcc
     4621 cgccggtcca cgtcgttctt gcgggcatcg agaagctggt ccccacctac gaggacgcaa
     4681 tgacccagct ccggctgctc gcccggagtg caacggccca gcgcttgacc gtctactcaa
     4741 ccttcattca gggaccgagc cccggccatg agcagcacat cgtcctcgtc gacaatggac
     4801 gtcggaccat gcgcgacctc cccgagttcc gtgaggcttt gcactgcatc cgttgcgctg
     4861 cctgcgccaa tgtctgcccg ccctaccgag aggtcggcgg ccacgtcttt ggctatatct
     4921 actccggcgc gatcgggctc gtcgtcaccc cattcatgca cggaatcgac gccgctgctg
     4981 gcccgcagag cctttgcctc tcctgcaacg cctgtgaggt cgtctgcccg gtggagattc
     5041 cgctgccccg gcagatcctc gatgtgcgcg ccatggtggt gaaacagaag gggatgaagc
     5101 ggagcaagca ggttattctc tc
//
LOCUS       DASYWM010000304         5496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_672161, whole genome shotgun sequence.
ACCESSION   DASYWM010000304 DASYWM010000000
VERSION     DASYWM010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672161"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..290)
                     /locus_tag="VGR29_12105"
     CDS             complement(66..290)
                     /locus_tag="VGR29_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074373.1"
                     /translation="MAITKAQAAGLSNDMLLGGVIETIVKESAILSFLPFMEVTGTAL
                     SYNREVTMPDVNFYASGDTSAEATPTFTKA"
     gene            complement(294..653)
                     /locus_tag="VGR29_12110"
     CDS             complement(294..653)
                     /locus_tag="VGR29_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074374.1"
                     /translation="MPELISPLELGQEDGSPDSHDASQASDLEAVREMVLRAHPDVVP
                     ELVAGDSVAALLASVEPTRSAYARLAEAWSDAQPQPVSVPAGGGAPLPIDPEKIPSAE
                     KIRRGLQAANRRTSQEG"
     gene            962..1273
                     /locus_tag="VGR29_12115"
     CDS             962..1273
                     /locus_tag="VGR29_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074375.1"
                     /translation="MSTRNPRPERDETVAGESVVPEQSAAREIVRPEHTRTRNVPPRE
                     EHGENVGPDEPIRFDQNDDVVPPRETESPARAPIVATAMFLPVVILSVVALVLILWFL
                     L"
     gene            complement(1382..2404)
                     /locus_tag="VGR29_12120"
     CDS             complement(1382..2404)
                     /locus_tag="VGR29_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2074376.1"
                     /translation="MGIKIGFTLPNRGVLFGATTSRELVDLAEIADKSGGFNSVWVGD
                     SLFGKPRLEAITLLAAIAARTESVRLGPACMASFPLRDPIQLAYQWASLDLIAEGRTV
                     LVACTGIVPQAGGEIEAKTYNVTNRDRVQRLTEGIQVLKKLWTEDNVSFTGEQYSFEN
                     VTIEPKPAASPRPPIWIANNAPGTNRDLVSKTHRRVIKHADGWQTSKWEADDLAWRLK
                     DIDEQATELGRDPRSIERHLYHNVNLNDDRDAAIEESKKFLDLYYTTDYARDFVEGWV
                     ATGPVEQVVEHIKVYEALGFDEITLRITSWNQREQLDRLMREVLPHFGQSSGQRASTA
                     QKAAVD"
     gene            complement(2424..3275)
                     /locus_tag="VGR29_12125"
     CDS             complement(2424..3275)
                     /locus_tag="VGR29_12125"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2074377.1"
                     /translation="MPTGYDHIVIAVNDLEETIADYTSAGFTVTPGGEHKHGISHNAL
                     VTFQDGSYFELIAFRNGGEGHGTHWPITLQKGEGIVDYALRTSDLEQEVRHLRVSGLD
                     YSDPKDGGRFRPDGQRVDWQTIRYGEGASVPARLPFYCHDLTERTLRVPGGVNAVHAN
                     GVTGIAGVSVVVRDLDAASKDFVALTGDAGSDVQSTLPDVSGARRFAIGAAWVEVVQP
                     AAHASELRAYLENRGELPCQVTLVSPEGSGDVLSGQLTHGARLLVASEPGIPTGFTDA
                     DRSGSPK"
     gene            complement(3360..4310)
                     /locus_tag="VGR29_12130"
     CDS             complement(3360..4310)
                     /locus_tag="VGR29_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074378.1"
                     /translation="MKYWLGRILETIPLLIGVSLLTFAIFRMAPGDPVALVIDPTMVS
                     EEDRQAVREALGLDEPLPVQYVSMMTGLFTGELRSFKSKQPTTEIVMDALPVTIIVGG
                     AGLLIGLIVALPLGTLAARRPGGWVDRLVSLSMTASLAIPSFVLGLVLILLFTERWGM
                     LPGSGIAPIGTIGFAGLESLPYLIMPVAVVAFSPASIFARYLRDALGSVLQDDYVRTA
                     RAKGLSETVVFSRHAVRNALLPVVSLLNSLIPITLGGSVVIEMLFGLPGLGRVTTQAA
                     LTRDYPVVITAVLFVAAIAVITNLVIDLVYGWVDPRVRVE"
     gene            complement(4307..5236)
                     /locus_tag="VGR29_12135"
     CDS             complement(4307..5236)
                     /locus_tag="VGR29_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074379.1"
                     /translation="MSPLQSASSSIAPSLAYRALDVSTGSRQRTPLRFLRRLAAHPRM
                     TVGATIIIVVSALALFAPWIVERPPDIQNVTYRLAPPSSEYWLGGDSYGRDIFSRLLY
                     AGRVSLSVGLLSMILTVVIGVLVGALAGYYGGWIDRILMRLTEIVLVIPTFFLLILTV
                     ATFGRSIPLLILIIGVTSWPINARVVRGEIMRLRKYDFVVASRVVGGKDSWIVVRHLL
                     PHLIPIVIASATIRVANNILIESGLSFLGLGVQPPTPTWGNMIAEGTSLMRQAWWLVG
                     IPGATIFVVVLAFNLFGEGLRDFLDPRTRGRTR"
     gene            complement(5267..>5496)
                     /locus_tag="VGR29_12140"
     CDS             complement(5267..>5496)
                     /locus_tag="VGR29_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074380.1"
                     /translation="NPESDKLIIAAASTIDPEERKRLYYEWQEVIAQDIPHLWIGNPD
                     EIVAYSTGLVVPERPSAYLAWREIDEWYWAE"
BASE COUNT         1147 a         1821 c         1541 g          987 t
ORIGIN      
        1 tctgagtagg cagcctgcag gaagttgtcg acatcggcgt cgccaccgag aatcgtcagc
       61 gcggcctacg ccttggtgaa ggtcggcgtc gcctccgccg aagtgtcgcc cgaggcataa
      121 aagttcacgt ccggcatcgt cacttcccgg ttgtagctca gcgcggttcc ggtcacctcc
      181 atgaacggaa ggaacgagag gatcgccgac tccttgacga tggtttcgat cacaccgccc
      241 aacagcatat cgttcgagag gccagccgcc tgtgccttcg taatcgccac gcgttacccc
      301 tcctgtgacg tgcgccggtt cgccgcctgc aaaccgcgac ggatcttctc ggcagacgga
      361 atcttctccg ggtcaatcgg taacggcgct ccgcccccgg cagggacgga taccggttgc
      421 ggctgtgcgt cgctccatgc ctcggcgagc cgcgcatagg ccgaccgtgt cggctccaca
      481 ctcgcgagca atgcggccac ggagtcacca gcaaccagct ccggcacgac gtcaggatgc
      541 gcgcgcaata ccatctcgcg caccgcctcc agatcgctcg cttgcgatgc gtcgtgtgag
      601 tccggagaac catcttcctg acccagctcc aacggggaaa tcagctcggg cacttcactt
      661 ctccttgcga aacgggcaag aaacgcaacg ggaggacacc ctgtgacagg cgtcctcccg
      721 ttcggggata ccggacatcg cgggatgaac cggcgacgac cgatacgaac atctgttcgt
      781 atcggtcata actactatac gcgttcaccg ctcccgtatg cgccacaggc tgattggtgg
      841 caccccggtt gcaggaacag cagtctagac atcaagagtg atggatcgcc tcgtcaaact
      901 ctcaacatgc cacagagcat ccaacgagcg cagacccatc atcacaagat tggagcaaac
      961 catgagcacg cgaaacccgc gtcccgagcg ggacgaaaca gttgcaggcg aatcagtcgt
     1021 tccggagcag tcagctgcac gcgaaatcgt ccggccagag cacacccgaa cacggaacgt
     1081 accgccccgc gaggagcacg gtgagaatgt cggcccggat gagccgattc gtttcgacca
     1141 gaacgatgat gtcgttccgc cccgcgagac ggaatcacca gcccgtgccc cgatcgttgc
     1201 cacagccatg ttcctgccgg tagtgatcct cagcgtagtt gcgctggtcc tcatcctttg
     1261 gtttctgctc tagggcacca cgtccataac atcccgggcg caagaaggag gcggcccccc
     1321 tgggccgcct tttcgttgat acttgctgga ccggtttcgt gggccaatcg ctgcacctgg
     1381 cttaatcaac cgcagccttt tgtgccgtcg atgcgcgctg gcccgacgac tggccaaagt
     1441 gtggaagcac ctcccgcatc aggcgatcca actgctcacg ctggttccat gaagtgattc
     1501 gcagggtgat ctcatcaaat cccagcgcct catagacctt gatgtgttcc accacctgct
     1561 ccaccggccc ggttgccacc cagccttcaa cgaagtccct cgcatagtcc gtcgtgtagt
     1621 agagatcgag gaattttttg gactcctcaa tcgccgcgtc cctgtcatcg ttcagattga
     1681 cattgtgata gaggtgacgc tcgatactgc gaggatcccg ccccagctcc gtcgcctgtt
     1741 catcgatgtc cttcagtcgc caggcgagat catcagcctc ccatttcgag gtctgccagc
     1801 catccgcgtg cttgatcacg cggcgatggg tcttgctcac cagatcccgg ttggttcccg
     1861 gcgcgttgtt cgcgatccag atcggtggtc gcgggctggc agcgggcttt ggctcgatcg
     1921 tcacgttctc gaacgagtac tgctcgccag tgaagctgac attatcctcg gtccagagct
     1981 tcttgagcac ctggatgccc tcagtcagcc gctgaacacg gtcccggttc gtgacgttat
     2041 aggtcttggc ctcgatctct ccaccggcct gcggaacgat acccgtgcaa gccaccagca
     2101 cggttcggcc ttcggcgatc agatccaggc tcgcccactg gtaagcaagc tggattggat
     2161 cgcggagcgg gaagcttgcc atgcatgctg gccccagccg cacgctttcc gtacgcgccg
     2221 caatcgccgc cagtaaggta atcgcctcta gacgcggctt accgaacagg ctgtcaccca
     2281 cccaaaccga gttgaagcct ccagacttgt cggcaatctc cgcgagatca acaagctccc
     2341 gggaggtcgt tgcgccgaac agcacgccgc gatttggaag cgtgaaacca atcttgatac
     2401 ccatgcgaga cgctctcttt catctatttc ggcgaaccgg atctatccgc atccgtgaat
     2461 ccggttggta tcccgggctc agaggcaaca agcaaccggg caccatgcgt gagttgcccg
     2521 gagagcacgt caccgctccc ctctggcgag acgagggtga cctggcacgg cagttcgccc
     2581 cggttctcca gatatgcccg gagctcggag gcatgcgctg ccggctgaac cacctcaacc
     2641 caggcagcac caatggcgaa ccgcctcgcg ccggacacat cgggtagcgt gctttgtaca
     2701 tcgctaccgg cgtcaccggt cagcgccaca aagtccttgc ttgccgcatc caggtccctc
     2761 accaccacag acaccccggc aatcccggta acgccgttcg catggaccgc gttcacgcct
     2821 cccggcaccc gcagcgttcg ctcggtgagg tcgtggcagt aaaagggaag ccgcgccggc
     2881 acactcgcac cctctccata ccggatggtc tgccagtcca cccgctgacc gtcggggcgg
     2941 aaccgccctc catcctttgg atcgctataa tcgaggccac tcacccgcag atgccggact
     3001 tcctgctcca ggtcactggt gcgcagggcg taatcgacga tcccctcgcc tttctgcagc
     3061 gtgatgggcc agtgcgtgcc atgtccctca ccaccattgc ggaaggcgat caactcgaag
     3121 tacgacccgt cctgaaaagt caccagcgcg ttgtggctga tgccgtgttt gtgctcacca
     3181 ccaggcgtga ccgtgaaacc ggcactcgtg taatccgcga tggtctcctc gagatcattc
     3241 acggcaatga cgatgtgatc atatccggta ggcatctgtc cctcccctgc atcgcaaaac
     3301 gttgagatct ccgttaaaag gcccgctcct ttacgcctgg gtccgggcga acggcccggt
     3361 tactcaaccc tgaccctggg gtcaacccat ccatacacca gatcgatcac aaggttggta
     3421 atgaccgcga tcgcagccac gaaaagcaca gcggtgatca caacggggta gtcacgagtc
     3481 agggcggcct gagtggtgac tcgcccgagc cccggcagcc caaacagcat ctcgatcacg
     3541 accgaccccc cgagcgtgat cgggatcagg gagttgagca acgacaccac aggcagcagc
     3601 gcattccgga ccgcatggcg cgagaagacc accgtttcac tgagcccctt cgcccgggca
     3661 gtacgcacgt aatcgtcttg caacacgctg cccagggcat cgcgaaggta gcgagcgaag
     3721 atggatgccg gactaaacgc cacgactgcc accggcatga tcaggtatgg cagggattcg
     3781 aggcctgcga atccgatcgt tccgattggc gcgatccccg agccggggag cataccccac
     3841 cgctcagtga aaagcaggat cagcacgagc ccaagcacga acgagggaat ggccaggctt
     3901 gccgtcattg agagcgacac caaccggtca acccaaccac cgggtcttcg cgccgcaagc
     3961 gttcccaggg gcaacgcgac gatcaggccg atgagcaacc ccgcaccgcc gacgatgatg
     4021 gtgacaggaa gggcgtccat gacgatctcc gtcgtgggtt gctttgactt gaacgaacga
     4081 agctcgcccg tgaacaaccc cgtcatcatt gacacgtact gcacgggcag gggctcgtcg
     4141 agccccaaag cctcacgaac cgcctgccgg tcctcctccg acaccattgt cgggtcaatc
     4201 accagcgcca cagggtctcc cggagccatg cgaaagatgg caaacgtcaa cagcgagact
     4261 ccgatcagca gcgggatcgt ctccaggatc ctccccaacc agtacttcat cgggttctgc
     4321 ctcgcgtgcg tgggtcgagg aagtcacgca atccctcgcc aaagagattg aacgccagca
     4381 ccaccacgaa gatcgttgcc cccggaatgc cgaccagcca ccatgcctgg cgcatgagac
     4441 tggttccttc agcaatcatg ttcccccatg tcggcgttgg aggctggact ccaagtccca
     4501 ggaagctcag ccccgactcg atcaggatat tgttcgcaac ccggatcgtt gcgctggcaa
     4561 tcacgatcgg gatcaaatgc ggcagcagat gacgcaccac gatccacgag tccttacccc
     4621 cgaccacacg cgacgccacc acgaaatcgt acttccgcag tctcatgatc tcccccctca
     4681 ccacgcgggc attgatcggc catgaggtga cgccgatgat caggatcagc agcggaatgc
     4741 tccgtccgaa cgtcgcaacg gtcaagatga gcaggaagaa tgtcgggata accagcacaa
     4801 tctcggtcaa tcgcatgagg atgcggtcga tccagccacc gtagtacccg gccagggcgc
     4861 caacgagcac gccgatcacg acggtgagga tcatcgacag caatccgacg gagagggaca
     4921 cccgccctgc gtacagcagc cggctaaaga tatcgcgacc ataggagtca ccacccagcc
     4981 agtactcgga agacggcggt gccaagcgat aggtcacgtt ctggatgtcg ggaggacgtt
     5041 caacgatcca tggagcgaac agggcgagcg cggagacgac gatgataatc gtcgccccca
     5101 cggtcatccg cggatgtgcc gccaaccggc gcagaaagcg tagcggtgtg cgctgacgcg
     5161 agcccgtgga gacgtccagc gcgcgataag ccaggctcgg cgctattgag cttgaggcac
     5221 tctggagtgg gctcatgttt gtatcctgat ccgggcccgc cacaagctac tcagcccaat
     5281 accattcgtc aatctcccgc cacgccaggt aggcgctcgg ccgctccggc accaccaacc
     5341 cagtcgagta ggcaacaatt tcatccggat tcccgatcca gagatgcggg atatcttgcg
     5401 cgatgacctc ctgccactcg taatagaggc gtttgcgctc ctccgggtca atggtcgacg
     5461 ccgcggcgat gatcagcttg tcggactccg gattgt
//
LOCUS       DASYWM010000305         8158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_672182, whole genome shotgun sequence.
ACCESSION   DASYWM010000305 DASYWM010000000
VERSION     DASYWM010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672182"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(514..587)
                     /locus_tag="VGR29_12145"
     tRNA            complement(514..587)
                     /locus_tag="VGR29_12145"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(551..553),aa:Asp,seq:gtc)
     gene            complement(843..1967)
                     /locus_tag="VGR29_12150"
     CDS             complement(843..1967)
                     /locus_tag="VGR29_12150"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV2074381.1"
                     /translation="MKTLVEASAAFGQGAGIGRYARNIVTRLISASDDEWALVRAPAN
                     RSEASFFDPVRSDRVKVVTLPFSRRNADRLWFRMRLPVDIRLFGGRGDVVYSPDFTAP
                     PMLGVPRMITVHDLAFMTHPGHTTEALRRYLEGVVPREVARADKVAVVSHATRKDVVR
                     LLGVPEHKVVVVANGVDDRFYQAAPLASAARKRLGLPERYLLMVGTIEPRKNHLNAMR
                     ALERSRVGNHLPLVIAGRTGWGYEETMEQARRLVNRGLVQLLDYFPDEDLPGLYAGAK
                     AVLYPSWTEGFGLPVVEALASGTAVVTGTAPALREVGGDEAIYADPGDVEGLADRIRH
                     VAEQESSDEDRARRQQWTRRFSWDSSTSTVIESLKNLSRR"
     gene            complement(1951..3060)
                     /locus_tag="VGR29_12155"
     CDS             complement(1951..3060)
                     /locus_tag="VGR29_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV2074382.1"
                     /translation="MTTESTKFLHDRPVEVDSVGAIHDATMEREPDGQDPAPDSLGRR
                     FLNLRTLVSFGIAIAILFFVFRGLGIDLGDAWGRMKQANLVLLAGALAIFYSTLAVRA
                     VRWRYMLARAGISRAQGYPVPALPGMFQIILLSWFANCVVPARLGDAYRSFLLKERTS
                     ASFGMSLGTILAERLIDLVMLVTVLLGSGLVVFGTQAPDQAETAFLFGGGVVLFGVVG
                     VVVLWLLRDRIEQLLPVRVMFHYRKLNAGIFRVLRKPIPYAATSVLLWLGDGLRVYLV
                     AWSLGQRISYQAAIMVALISALVSVIPFTPAGLGFVEGFMVTALTQIGVPPGAAIAIA
                     LLDRIVTYFSLIVFGLPLYLWALRSDLKGVRENPG"
     gene            complement(3057..4523)
                     /locus_tag="VGR29_12160"
     CDS             complement(3057..4523)
                     /locus_tag="VGR29_12160"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV2074383.1"
                     /translation="MQAVRSDPAEAFRPRDVIAVLILLAVILGMLGIPNSIGATVVLM
                     GTGILLALIAPAVMVSLVVMTVPVQDSVVLPFFRGELTLTQIAVSAMIVGWVMALRRY
                     RVWLDSITLGFLAIGAAFLVSFIATDDLGLWAGEVYRWVIAAVFFVICRSVLRDWQSV
                     RAVLVAIAASVSAVGAYGIGQISAYRSGDLVVGGVLRVFATFGKPNPLAAYIEFTLPV
                     LLLLVLLGFQRHFRQRIGTMLWLLSGFATGMGIVILTLTQSRGGMLGFAAGTLIILMV
                     LPVRLRLAAIGTGLLLVAIMATTPSGQSQLERFGDALSESEQVQTGSTFNYGTGRSAI
                     WATGSRMFEDEPWTGVGAGEFDYHYREYAAVWYDRFPIGQAHNGWLQMGAQAGIPGVL
                     AFTGWVAAALVSLIGAVRRSIDPRSRALALGALAIMIAFTVHSLVDYLNVLSLGLQLS
                     CVLAIGLNLAPDPLNCAHGKHATGPSMSVAVPLEAEPA"
     gene            complement(4504..5937)
                     /locus_tag="VGR29_12165"
     CDS             complement(4504..5937)
                     /locus_tag="VGR29_12165"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV2074384.1"
                     /translation="MAEFRSDSWRHTPSFMVLASFSLVVIGAVIWTGIASTLFVAVAL
                     LGTAVLLAALLPAAGVAGVLAALPTMYELHPMPRGQFSLLELAILVAAAGTFLNVLAQ
                     LRNRSWDVIRSLVVPAQIVVPVLLLALAAGVSLLTIADPAHRAESLREVRTVIVEPLI
                     FLATARLVLRDTIWRNWVGASFILVGLVIAIGALVQVAFNLGGVAAGSVLRATGPYTH
                     PNNLALFLERTLLFTVAVAFLKPRWWPVWGIVAIQLLGVGATYSRGALLAVAISIGFL
                     MLLLGMHRWLGALAIGGLVVGGMAFLLAPERLIDAGGSGSEPTRFALWRSSVRMALDH
                     PVFGVGPDQFLYQYWRRYVEPIGWPERYSSHPHNLVLDVWLRLGVVGLTAFATLIVGL
                     IWWVRRSFDCIRGDIWSMGGIAALVGGLAHGMVDQGFFLPDLATMTWLFVSFLITTAT
                     AKPPGFSSPPARPRNEPIGRVDASRTF"
     gene            complement(5937..7748)
                     /locus_tag="VGR29_12170"
     CDS             complement(5937..7748)
                     /locus_tag="VGR29_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074385.1"
                     /translation="MVRPYPLDPDDSDRGRKLNAAGQGAGRAGLGAQLAALTADAIVA
                     LLDLRHRRPDLTHFLGALLLLLVGIVLIASTSLTDQYLHRGVESGAERPYVVQPSGRE
                     LATNVDLRIFTPEDIRDVAATLGDAGFGYVRQEFSWAEVETDQGQFNWSLYDPIVEAM
                     NQESIAVIAVIVDAPRWSVAGETVISNTRPPADPGDLQRFARELTAHYGDRVPYVQVW
                     DRPNLASEWGGRPATGATFQPYLAAGFYGAREGNAAVQVITPELAVMPDVAEGVSDLA
                     FLRGLYRADAEDVFDIVGIALDGGTNSPDDRRVSVERENFSRAILTRELMVRHGDADT
                     PVWATTYGWPVGSEVSREEQAEYVVRGLERAWSEWPWMGLMIQWSFAARAEDPESKYA
                     VAPGGSSTPLYRRLTDSDLRRRSDIANTGFAPMNAEAVTFEGIWDDQHLEGRTFRTTN
                     QVGSSTTLRFQGTGLIAFLRSGPQAGAFRLELDGEIIPGGYGDEENLWSFFVSFRTDD
                     LPRRLVSGLDDEEHTLRITLMEPGELTLGGFVVEREAPFVWPIILLTTISVIVLFLGL
                     RFMIYLVAIRSGHLQRRADAPFPALPRMPDWQPGRRA"
     gene            7965..>8158
                     /locus_tag="VGR29_12175"
     CDS             7965..>8158
                     /locus_tag="VGR29_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074386.1"
                     /translation="MTVQSGKRPITADDIKQIKLVSDPQAQPNGDLVTWVVTRVDDDG
                     DTYTSGLWIARSNGENARQLT"
BASE COUNT         1960 a         2526 c         2263 g         1409 t
ORIGIN      
        1 ctgctaccgc gggcggcaag gaggcaaatg cagtcagcgc tcgaccttcc ttgatgtcta
       61 tgaaactcag ctgatgaact acttggctac atttacgatc ccagaaagct accgagaggt
      121 actcctcgct tcgcagacgc ggacacgtgc aaacgccgac gatgatgagg tgcaacggcg
      181 acggcttgag gcgcaattgc tcaagctgcg gacattgttt gagcttggtg attacaccaa
      241 agcagagtac ctggagcgca gggaacagtt gcagcgatct ctggaagcgg caaagacgcc
      301 cggggaggtt ggggaagctc tggaacgagc agcaacattc ttgagcgatt taccggcagc
      361 atggggcgcc gcaactgacg atcaacgcaa tgtgctcgcg cggctgctgt tcgaagaggt
      421 tcacattaaa gacgaatggg ttgcagcagt taagccacaa cccacctttg caccgttttt
      481 caacctcgac tgtcaatctc gacgtctatc cggcggaagc gacgggattc gaacccgcga
      541 tctcagcctt gacagggctg catgttaggc cactacacca cgcctccaac agccccgaaa
      601 gagtaccaaa ggttccgcgg tgcgtcaacc tccgggtgaa ctcagccgcg cgcaacggac
      661 ggctcgaaga ttcgactatc gattgacgta cagcgcccaa gcccaaatac agagcagcag
      721 gaagttgagc ataaaaaacc ccatcccgat caaaaggggc ttcacgcgcg gcgatggtgg
      781 ccagaactcc gatagcgtga gtttcggtag gggctcaagc tccttttcgg actccgctcc
      841 atctacctcc gactcagatt cttgaggctt tctataacgg tagaggtcga agaatcccac
      901 gaaaaccgcc ttgtccactg ctgcctgcgt gcccgatctt catccgacga ctcctgctcg
      961 gccacgtggc gaatccgatc tgccaaacct tcaacatcac cggggtcagc atatattgcc
     1021 tcgtccccac caacctctcg cagcgcgggt gcggtgcccg tgacgacggc tgtgccagat
     1081 gcaagtgctt cgacgactgg aaggccgaac ccctcggtcc aggaaggata gagtacggct
     1141 ttcgcccctg catagagccc gggcaggtcc tcatccggaa agtaatccag gagctggaca
     1201 agtccccggt tcaccagccg ccgcgcttgc tccatcgtct cttcatatcc ccatccggtg
     1261 cgaccagcga ttaccaatgg aagatggttc cccacccggg atctctccag tgcccgcatg
     1321 gcgttcaggt ggtttttgcg cggttcgatc gtgcccacca tcagcaggta acgttctggc
     1381 agtcccagtc tctttcgcgc cgcgctagcc agtggcgccg cctggtagaa ccgatcatca
     1441 acaccgttcg ctacaacaac gaccttgtgc tcagggaccc cgagcaggcg gacgacatct
     1501 ttccgtgtgg catgcgagac gacagccacc ttgtcggcac gagcgacctc acgaggtacc
     1561 acgccctcga gataacgacg gagggcttcg gtggtatggc cgggatgcgt catgaaggca
     1621 agatcgtgca ccgtgatcat ccgcggcaca ccgagcattg gcggtgccgt gaaatccggg
     1681 gagtagacga catctcccct gcccccgaat agccggatat caaccggcag tcgcatccga
     1741 aaccacaggc ggtcggcgtt tcggcggctg aacgggagcg tgaccacctt tactcgatcg
     1801 gacctcaccg ggtcgaaaaa cgaagcctca gatctattag ctggcgcgcg aaccaatgcc
     1861 cattcatcat cgctggcgct gatgagccgg gtgacgatat tgcgcgcgta gcgccctatt
     1921 cccgctccct gaccgaaggc agcactcgcc tcaaccaggg ttttcacgca cacccttgag
     1981 atcgctccga agggcccaca gatacagggg gagcccgaac acgatgaggc tgaagtacgt
     2041 cacaatgcgg tccagcaagg caatcgcgat tgcagcacct ggcggcacac caatctgggt
     2101 gagggcagtc accatgaacc cctcaacgaa cccgagtcct gccggggtga agggaatcac
     2161 tgacacaaga gcactgatca gtgcaaccat gattgccgct tgataagaga ttcgctgccc
     2221 aagcgaccac gcgacgaggt agacccgtaa tccatcgccc aaccagagca gcacgcttgt
     2281 tgccgcatac ggtatcggct tcctcaaaac cctgaagatc ccggcattca atttccggta
     2341 atgaaacatc acgcgtacgg gaagcaactg ctcgatgcgg tcccttagga gccaaagcac
     2401 cacgacccct acgacgccga acaggacgac gccgcctccg aacaggaagg cggtctccgc
     2461 ctggtctggg gcctgtgtgc cgaagacgac aagtccggag ccaagtagga cggtgacgag
     2521 cattacgaga tcgatgaggc gttcagcgag aatggtgccg agagacatcc cgaacgaggc
     2581 cgaagtgcgc tccttgagaa ggaagcttcg ataagcgtca ccgagtcgag cgggaaccac
     2641 gcagttggcg aaccaggaga gcaggatgat ctggaacatg cccggaagtg cgggaacagg
     2701 atacccctgc gctctactaa tgcccgcacg ggcgagcata tatctccatc gcacggcgcg
     2761 aacggcgagg gtcgaataga agatcgccag agcaccggcg agcaacacga gattggcctg
     2821 tttcatcctc ccccatgcgt ctcccagatc gattcccaaa cctcgaaaaa caaagaagag
     2881 aatagcgatg gcgatcccga aggacaccaa agtacggagg tttaggaagc gtctgccgag
     2941 tgaatcgggc gctggatcct gtccgtcggg ctccctttcc attgtcgcgt cgtgaatcgc
     3001 gccgacggaa tcgacctcga cgggccggtc atgaaggaac ttcgtcgatt cggtggtcat
     3061 gcaggctcgg cttctagcgg cacggccacg gacatagagg gaccggtggc atgcttcccg
     3121 tgagcacaat tgagcggatc aggtgcaaga tttagcccta ttgcgagaac gcagctgagc
     3181 tgcagcccca ggctgagcac gttgagataa tcaaccaggc tatgtacggt aaacgcgatc
     3241 ataatggcta gcgcccccaa tgccaatgct ctggaacgag gatcgatact ccgccttacg
     3301 gcgccgatca aggataccag cgctgccgcc acccatcccg tgaacgcgag aacgccagga
     3361 atcccggcct gtgcccccat ctgtaaccag ccattgtgtg cctggccgat cgggaaccgg
     3421 tcataccata cggcggcgta ctcccgatag tgatagtcga actcaccagc accgacgccg
     3481 gtccagggtt catcctcgaa catacggctt cccgttgccc aaatagctga acgaccagtt
     3541 ccataattaa aagtagaacc cgtctgcacc tgttccgatt cgctcagagc atcgccaaat
     3601 cgttccagct gagattgccc tgaaggagtc gttgccatga ttgcaaccag caacaatccc
     3661 gtaccgattg cggccagtct cagccgaacc ggcagaacca tgagaataat caacgtcccc
     3721 gcagcgaacc ccaacatccc tcctctggac tgcgtgagcg tgagaatcac aatgcccatt
     3781 cccgtagcga acccagagag cagccagagc atcgtgccga tccgctgcct aaagtgccgc
     3841 tgaaatccaa gcagcaccag aaggagcaag acgggtaatg tgaattcgat gtaggccgca
     3901 aggggattgg gtttgccgaa tgttgcaaat actcgcaata cgcctcctac tacaagatcc
     3961 cctgaccggt atgcggagat ctgaccaatg ccataggcac cgacggcact gacgcttgca
     4021 gcaatggcaa cgagcactgc tcggacagat tgccagtcac gcagcacgct acggcagatt
     4081 acaaaaaaga ctgcggcaat cacccaccga tacacttctc ccgcccagag cccgaggtcg
     4141 tccgtcgcga tgaaagaaac aagaaatgcg gctccgattg ccagaaaccc cagagtgatg
     4201 ctatcgagcc acacccgata tcgccgaagc gccatcaccc accccactat catggccgac
     4261 actgcgatct gggtaagggt caattctccc cggaagaacg gcagcaccac gctgtcctgc
     4321 accggcaccg tcatgacaac caaagaaacc atcacggccg gggcgatcaa cgcaagcaaa
     4381 ataccagtac ccatgagtac cacggtggcc cctatagaat tcggaatgcc gagcatgccg
     4441 aggatcacgg ctagaaggat gaggacggcg atcacgtccc ttggccgaaa cgcctcggcg
     4501 ggatcagaac gtacggcttg catcgaccct cccaatcggc tcattcctcg ggcgggcagg
     4561 tggagaggaa aaaccgggag gtttcgctgt ggcggtggtg atcaggaagg agacaaatag
     4621 ccacgtcatc gtcgcgagat ccggcaggaa gaatccctgg tccaccattc cgtgggccaa
     4681 gcccccgacg agcgcggcaa tgccgcccat gctccagata tcgccgcgaa tgcagtcgaa
     4741 cgaccgcctc acccaccaga tgagtccgac aatcaacgtc gcaaacgccg tcagacccac
     4801 gacacccaat cgcaaccaca cgtcaagcac gagattgtgc ggatgtgaag agtatcgctc
     4861 cggccagcct atgggctcga catagcgccg ccaatattgg tagagaaact ggtccggtcc
     4921 gacgccaaat accgggtgat ccagagccat ccttacgctc gatcgccaaa gcgcaaagcg
     4981 agtcggctcc gaacccgagc ctccggcgtc aatcaatcgt tcaggggcaa gcaggaacgc
     5041 cattccccca acaaccagac ctccaatcgc cagtgcgccg agccaccggt gcatcccaag
     5101 caggagcatc agaaagccaa tggatatcgc gaccgcaagc agggcacccc gcgaataggt
     5161 cgcgcccact cccagaagct ggatcgcgac aataccccag acaggccacc aacgaggttt
     5221 gaggaatgcg actgcgaccg tgaacagaag ggtgcgttca agaaacagcg ccaggttgtt
     5281 tggatgtgtg tatggcccgg tggccctcag aaccgatcct gcggccacac cgccgaggtt
     5341 gaatgcaacc tggacaaggg caccaatggc gataacgagt ccaactaaaa tgaaggatgc
     5401 tcccacccag ttgcgccata tcgtatcgcg aagcaccaat cgcgctgtcg cgaggaaaat
     5461 gaggggctca acgataaccg tgcgtacctc tcgcagactc tcggcgcggt gggcggggtc
     5521 tgcgatcgtc agcaaagaga cacccgccgc aagcgccagc aatagtaccg gaaccacgat
     5581 ttgcgccggc actaccagtg aacgaattac atcccatgat cggttgcgta gctgggcaag
     5641 gacgttcagg aatgtacccg cggcggcgac aagaatcgcc aactccagca aactgaattg
     5701 gccacgcggc atgggatgca gctcgtacat cgtgggcaac gctgcaagga ccccggccac
     5761 tcctgccgct ggcagaagtg ccgccaatag cactgcggtc cccaacaatg ccactgccac
     5821 aaaaagtgtg gatgcgatgc ccgtccagat gacggcaccg atgaccacca gcgagaatga
     5881 tgcaagaacc atgaatgaag gagtatggcg ccaggagtcg gaacgaaatt cagccatcaa
     5941 gcacgcctgc ccggttgcca atctggcatg cgggggagcg ccggaaatgg cgcatctgcc
     6001 cgacgctgca aatgcccgga ccgaatagca acaagataga tcataaatcg caggccgagg
     6061 aagagcacga taactgagat cgtggtaagg agaatgatgg gccagacaaa gggtgcctct
     6121 cgctccacca caaatccgcc cagtgtcaat tcccccggct ccatcaaggt gatccgcaac
     6181 gtgtgctcct catcgtcgag accgctgacg agtcgacgtg ggaggtcgtc cgtccggaac
     6241 gaaacaaaaa agctccagag attttcttca tcaccgtagc cgccaggaat aatttccccg
     6301 tcgagttcga gcctgaatgc gccggcctgg ggacctgacc ggaggaacgc gatgagcccc
     6361 gtcccctgaa acctcaatgt ggtgctggaa cctacctgat tggtcgtgcg aaacgtgcgc
     6421 ccttcaaggt gctggtcatc ccagatgccc tcgaatgtca cggcctctgc attcatcggg
     6481 gcaaaacctg tgttggcgat atcggaccgg cgccggaggt cggaatcagt taatcgacgg
     6541 tagaggggag ttgaagagcc acctggagca actgcatatt tcgactcggg atcttccgcc
     6601 cgtgccgcga acgaccactg gatcatgagc cccatccaag gccattccga ccacgcccgc
     6661 tccaatcctc tcacgacata ctccgcctgt tcctcgcgag aaacttcgct tccgaccggc
     6721 cacccgtagg tcgtcgccca gacaggcgtg tccgcgtctc cgtgccgcac catcaactcc
     6781 cgtgtcagga tcgcccgcga gaagttttct ctttccacac ttactcggcg atcgtccggg
     6841 gaattggtgc caccatcaag ggcgataccg acaatatcga agacgtcctc cgcatcggct
     6901 ctgtacaacc ctcgaagaaa tgcaaggtcg ctgactccct cggcgacatc cggcatcacg
     6961 gcaagctctg gagtgatgac ctggactgcc gcatttcctt cccttgcacc atagaatccg
     7021 gccgccaggt atggttggaa tgtcgcgccc gtggcgggcc gaccgcccca ttcactcgcc
     7081 aggttcggcc gatcccagac ttggacatac ggcacccgat caccatagtg cgccgtcagt
     7141 tccctggcga agcgctgcag gtcaccgggg tcggcaggag gacgagtgtt gctgatcacc
     7201 gtctcgcccg ccacggacca acgcggagca tcgacgatga ctgcaattac tgcaatgctc
     7261 tcctggttca tcgcctccac gatcggatcg tagagggacc agttgaactg gccctggtcc
     7321 gtctccacct ctgcccagga aaattcctgg cgcacgtagc cgaatccggc atcgccgagt
     7381 gtcgctgcaa catcgcggat atcctccgga gtgaagatgc gcagatcgac attagtcgcc
     7441 agttcgcgac cagacggctg aaccacatat gggcgctcag cacctgactc cacaccgcga
     7501 tgtaggtatt gatccgtcag tgaagttgaa gcaatcaaca caataccgac gagaagtaac
     7561 agcaacgcgc caaggaagtg agtcagatcc ggccggcgat ggcggagatc cagcaacgcg
     7621 acaatagcat ctgccgttag agcggccaac tgcgctccga ggccagcacg cccggccccc
     7681 tgccctgccg cattcaattt tcggccgcgg tccgagtcgt cgggatcaag cggatatggt
     7741 ctcaccacaa gggcagttct cttggatata cagtctggcg ggtgcaggaa tcgaccagtc
     7801 accctcgacg caaatcggcc atggggtgcg ctcaagtgcg ccacgaaagt atagcgcccg
     7861 gtatcgatgc cactgttgag agcacgcgtt gtctccggta tgctcccttg cagtccacga
     7921 tgcaggggcg aatcttccac ctgcccaatg aaggaacgac atacatgaca gtccaaagcg
     7981 gcaaacgacc gattacggcc gatgacatca agcagatcaa gcttgtctcg gacccacagg
     8041 cgcaaccgaa tggtgatctg gtgacatggg ttgtcactcg cgttgatgac gacggtgaca
     8101 cctataccag cgggctctgg atcgcgagga gcaacggtga gaatgcgcgc cagcttac
//
LOCUS       DASYWM010000306        13384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_53223, whole genome shotgun sequence.
ACCESSION   DASYWM010000306 DASYWM010000000
VERSION     DASYWM010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13384
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53223"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /gene="gcvT"
                     /locus_tag="VGR29_12180"
     CDS             <1..1072
                     /gene="gcvT"
                     /locus_tag="VGR29_12180"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEV2074387.1"
                     /translation="LGARIIPFAGWEMPVQYEGIIAEHRAVRSRAGLFDLGHMGQFAV
                     AGPDALAFLQYTTTNDLSTLDPGQAKYTLLSNENGGVIDDLIVYRNPEGEDGYMVVMN
                     ASNGDRDVDWWQEMRAKRDDMNVSLQNTSPEIGMIAIQGPHAEEITQKIASIPLGNID
                     YFAWQNGEIAGVSTKIARTGYTGEDGFEFYASNDEIGAVWDALMDAGKDSNLVPVGLG
                     ARDTLRLEARMPLYGQELGDDISPYEAGVGWAVSLDKGDFVGRDRMAQVKEQGPERRT
                     VGFRMTGRGGAPRTHYPVQLDGEEIGEVTSGAMSPTLGENIGLALIKRSAAGVGKPLQ
                     VIVRGKPVDAVQVKTPFYKRKS"
     gene            1131..1514
                     /gene="gcvH"
                     /locus_tag="VGR29_12185"
     CDS             1131..1514
                     /gene="gcvH"
                     /locus_tag="VGR29_12185"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEV2074388.1"
                     /translation="MSAPENRKYTKTHEWVLLEGDVATLGLTDHAQQELGDITYLELP
                     EAGDTVMSGEAFGVVESVKAASDVYSPIDGEVVERNDSAVDAPEVINQSPYDNAWLIK
                     VKVTDPAQLEGLLDPEAYNEYADQA"
     gene            1623..2978
                     /gene="gcvPA"
                     /locus_tag="VGR29_12190"
     CDS             1623..2978
                     /gene="gcvPA"
                     /locus_tag="VGR29_12190"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEV2074389.1"
                     /translation="MTFNPHTAEDREAMVQTIGVESVEELFAPVPERVRFPQLDLPPV
                     LTEMEAAHRLQQLADKNIVPPAEDIYLGAGSYQHYVPATVGQILSRGEFYTAYTPYQP
                     EVSQGTLQVIYEFQSMVADLLGMEVANASMYDGATALAEGALMSVASSKKKNRVVVAG
                     TVHPAYREVMHTYLDGSGVEIVEAAPKNPFVVTVDDIAPHVTDGLACVVVQYPNFYGA
                     IEDVQALADHAHGAGALFVVNTSPIPLGLLKAPGALGADIVAAEGQPLGVAQSYGGPY
                     VGLLGSKKSLVRQMPGRIAGVTTDSEGKRGFVLTLQTREQHIRREKATSNICTNQGLM
                     ATAATVYMSSLGPEGLREVARACYQHAHYLANRLTELEGFNLAYDQPFFHEFVVQTPG
                     PAAEVNAQLMESGILGGLDLGQLDDALSGHLLLCATELNSRAGIDRFIDIIGSDSNRF
                     R"
     gene            3098..4450
                     /gene="der"
                     /locus_tag="VGR29_12195"
     CDS             3098..4450
                     /gene="der"
                     /locus_tag="VGR29_12195"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV2074390.1"
                     /translation="MAKPIVALVGRPNVGKSTLFNRFIGERRAIVQNEPGTTRDRTYG
                     TADWVGVDFTVVDTGGLMDDDELKPGTSESEARIARETREQAFAAIAEADVVVFMTDV
                     TAGGPTAGDYEIAEILRRADKPTILAVNKADSVNRREVAFEYYELGLGEPMAVSAYHG
                     NGTGDLLDRVVENLPEMDEDEDELEGPRIAIVGRPNVGKSRLLNALLGQERAIVSDVA
                     GTTRDSLDTQIFWEGQPVTLIDTAGIRRRGRVEHGIERVSVMRAMRAIDRADVVVLVI
                     DAQEDFTSQDLHIAGYVEEQKKGLVVVVNKWDLIEKDHSTMDEYRARAARELDFMAYA
                     PVVFISAKLGQRVGQVIEKALEVLEQREKRISTAALNKVLKEAVSKHQPPSRPGKWVK
                     FFYATQADTAPPTFIFFCNDPKQIHFSYRRYLENELRQAFGFEGTPLRLSFRDRHEDL
                     "
     gene            4453..5082
                     /gene="plsY"
                     /locus_tag="VGR29_12200"
     CDS             4453..5082
                     /gene="plsY"
                     /locus_tag="VGR29_12200"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV2074391.1"
                     /translation="MDILRSVLLIAITYGLAGIPWGVVLGRLIKGVDLRSYGSGATGA
                     TNSLRVLGWQISVAVFILDFGKGFLPVLLARWIDLPGWGIALTAIVSVVGHCWSPYIG
                     FRGGKGMATGGGAAVALLPWLFGLLLFVVAVVYLTRYVSLASILTAIVGPLIVLVLWL
                     LGLFPAWWVVGVIGIGSIILWQHRGNIDRLRKGTERKFGRSESPSGNSA"
     gene            complement(5166..6143)
                     /gene="galE"
                     /locus_tag="VGR29_12205"
     CDS             complement(5166..6143)
                     /gene="galE"
                     /locus_tag="VGR29_12205"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV2074392.1"
                     /translation="MRVLVTGGGGYIGSVAIERLVARGHDVVALDDFSRGHAAAVHPN
                     ADKLHCDLRDAGSTAKAVATSGAEAILHFAALHLVPESVTNPGDYYATNVGGGINLLN
                     AARDAGISRFVFSSTAAVYGEPQSLPVRESSPKEPINPYGHSKWIVEQLLPVYAETYG
                     LNYAAFRYFNVAGATEERGEDHDPETHIIPVALQVLLGQRESFTVFGTDYPTPDGTAI
                     RDYVHVADLADAHITAIERLDSPLGAINLGTRDGFSVRQIVESINRVTGQELPIAFGE
                     RRAGDPPALIADTTRARELVDWNPTRSTMDEMIGSAWDWMQRHPNGYES"
     gene            6335..7588
                     /gene="glyA"
                     /locus_tag="VGR29_12210"
     CDS             6335..7588
                     /gene="glyA"
                     /locus_tag="VGR29_12210"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV2074393.1"
                     /translation="MISMDVLASADPEVARAVRQEEMRQIQGIELIASENYVSEAVLE
                     AVGTVLTNKYAEGLPGKRYYGGCEYVDVVENIAIDRVKQLLGAEHANVQPHSGASANL
                     AAFFSLLDPGDKILGMNLSEGGHLTHGKDVNFSGRFFEAHFYGVDQETGLIDYDDVLK
                     RAREVRPKAIISGASAYSRIIDFQRFREIADDVGAYLFSDIAHIAGLIAAGEHPTSVG
                     HAHITTSTAHKTLRGPRSGFILTDEELGPGVDKTVFPGMQGGPLMHVIAGKAVAFGEA
                     LQPSFRDYARQVKANAAAMARAFIDHGLPIISGGTDNHLFLVDVGSKGISGRKAERIL
                     DSVGISTNRNTIPGETRSAFQTSGLRIGTPAITTRGFGVEDSVQVAGFISRVIEAPED
                     ESVLAEVRRDVLELTAGFPVPGISR"
     gene            7631..8692
                     /locus_tag="VGR29_12215"
     CDS             7631..8692
                     /locus_tag="VGR29_12215"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEV2074394.1"
                     /translation="MSNGLIAAVVFIVTLMTSVAFVYKTTDLFSRLGLVDKPGPRRVH
                     STPVPRGLGVAIFLAFLVGVGATYFLPIPRQPQETERILLMVIGAAIVVGVMLVDDAI
                     SLGPGTKLTWQLIAAAVVILPRLRSIDHGMAIENLNLPFIGTLAIPVLIAIPVTFVWL
                     VGMMNVMNWVDGLDGLAGSVTLVSCAVMFVHTYWGPDNVPQFTISLLPLALGAAVLGF
                     LPFNWHPAKVIMGDAGAMFLGYALGVISIIGGAKIATALLVMGLPLLDGLWVTMYRIV
                     NGRSPLYADMGHLHHRLLAAGMGQRRIVLLVSAFSATFGALALLLPSRELKAAGLVLI
                     AFLLLSLLWWLAHKPQASK"
     gene            complement(9000..9380)
                     /gene="rsfS"
                     /locus_tag="VGR29_12220"
     CDS             complement(9000..9380)
                     /gene="rsfS"
                     /locus_tag="VGR29_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV2074395.1"
                     /translation="MTDHQSSMTDEIASSRALAVDIADIIAETPAADTRVIDIHGLTP
                     IADVFIICSGENERQLGAISRTITDEMAKAQIRPLRVEGDTTSGWILMDFGDVIVHVF
                     DVGLRAFYNLEDRWSEAPVLLSIQ"
     gene            complement(9439..9513)
                     /locus_tag="VGR29_12225"
     tRNA            complement(9439..9513)
                     /locus_tag="VGR29_12225"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9479..9481),aa:Ile,seq:cat)
     gene            complement(9681..10664)
                     /locus_tag="VGR29_12230"
     CDS             complement(9681..10664)
                     /locus_tag="VGR29_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HEV2074396.1"
                     /translation="MTDRIRITRGFAIATAIGMLIVLLMGARVTATGSADGCGNDWPL
                     CHGSFLPAGTYESLVEYSHRIVTGAEGILVAITSVLAWPMRRRYPEVKILIPVMAGTL
                     ILQSLMGAAAVKWPQTPEVLASHFGISLICLASAALLARVLTDRHPEKARHGEGALKP
                     QLPDHGVLRFRRLAVASVVISILVAYSGAYVRHRGAELACTSWPTCNGEIIPEFGGLH
                     GVQTIHRIGALIISLVILGLLTLGYRLREVRPDLFTISVLGMLLVVIQSLSGAVVIAS
                     GLQLMATLTHAAIMSLLFVTLCDGVRLTWKQRPEADLSRIGGELQGMAVGD"
     gene            complement(10887..11879)
                     /locus_tag="VGR29_12235"
     CDS             complement(10887..11879)
                     /locus_tag="VGR29_12235"
                     /inference="COORDINATES: protein motif:HMM:NF036726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEV2074397.1"
                     /translation="MVTASSDIFTQDDVFIRWHTHLVEIGAPPFTVTLPTGQDLLNTL
                     HYLEVPEEDVPHVLRLALSPKTDPELWWFLERAVHSLVLYMGKVPGPPRFAPLMDANH
                     PQYRFFYVHVFIAALPHVQQYFRQRSIPESVVQATLADLGRNVRVHRKRTGQGALGVA
                     WWLTLHFRGVIYQLGRLQFERARLGDRTVEQASRIGLGLSADDHVLSIHIPDFLGPLT
                     PEACDESIAEARTFFSTYFPDEPVVAGICNSWLLDPQLADYLREDSNIRRFQGRFQVL
                     PGGYNVNQSIVQFVFGPTTEDLDALPQRSSLERAVVAHLRAGKNWEGRTGWFPW"
     gene            12164..12616
                     /locus_tag="VGR29_12240"
     CDS             12164..12616
                     /locus_tag="VGR29_12240"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV2074398.1"
                     /translation="MSRISRRTLIAGSVLVPIAGIGLRGALAQDNESDATPGASPEAS
                     PMASPGASPMASPAAGSNEVTVIAIDIDFEQSEMRFPADTDVTVILENHGVLEHNWVV
                     DELDAETETIPGGETTSIVVNAPAGEYEYYCSVPGHKEAGMVGTLIVE"
     gene            12743..12818
                     /locus_tag="VGR29_12245"
     tRNA            12743..12818
                     /locus_tag="VGR29_12245"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12776..12778,aa:Gly,seq:tcc)
BASE COUNT         2765 a         3773 c         4072 g         2774 t
ORIGIN      
        1 gctcggcgcc cggattatcc cgttcgccgg ttgggagatg cccgttcagt atgaaggcat
       61 cattgcggaa caccgggcag tcaggagtcg ggctggtttg ttcgacctcg gccacatggg
      121 acagtttgcc gtcgccggtc ccgacgcgct tgcctttctt cagtacacca ccaccaacga
      181 cctgtccacg cttgatcccg gtcaggcgaa gtacacgttg ctctccaatg agaatggcgg
      241 cgtcatcgac gacttgatcg tctaccgcaa cccggagggc gaggacgggt acatggttgt
      301 gatgaacgcc tcgaatggcg atcgtgatgt cgactggtgg caagagatgc gggcgaaacg
      361 cgacgacatg aacgtttcac ttcagaatac gtctcccgag attggaatga tcgcgatcca
      421 agggccgcat gcggaagaga ttacacagaa gatcgcttcg ataccgcttg ggaacattga
      481 ttatttcgca tggcaaaatg gcgagatcgc cggtgtttcg acgaagatcg cgcgtactgg
      541 gtacactggc gaagacgggt tcgagttcta cgcctcgaac gatgagattg gagccgtgtg
      601 ggacgcattg atggacgcgg gcaaggactc taacttggtc ccggtgggtc tcggtgcccg
      661 tgacacgctg cgtctggagg cccgaatgcc tctgtatggc caggaactcg gcgacgatat
      721 cagcccgtat gaggcgggcg tgggttgggc cgtcagtctg gacaagggtg actttgttgg
      781 ccgcgatcgc atggcgcagg tcaaggaaca gggacctgag cgacgtaccg tcggattcag
      841 gatgaccgga cggggaggcg ctccgcgaac ccactacccg gtgcagttgg acggtgagga
      901 gattggtgag gtgaccagcg gtgccatgtc gccgacgctt ggtgagaaca ttgggctcgc
      961 gttgatcaag cggtcggctg caggtgtcgg gaagcctcta caggtcatcg tccgcgggaa
     1021 accggtagac gctgtgcagg tgaagacccc gttttacaag cgaaaatcat aagacgcgtt
     1081 ctttgaacgc cagagaatac ctatcacgac ttggaggagg agggatccac atgagtgcac
     1141 ctgagaatcg caaatacacc aagacccatg agtgggtgct gctggaaggc gatgtggcca
     1201 ctctcggcct aaccgatcac gcacagcagg agttgggtga tatcacctac ctcgaactcc
     1261 cggaagcggg agacaccgtt atgtccgggg aggcgttcgg ggtcgtcgag tcagtgaagg
     1321 ccgcgtcgga tgtctattca cccatcgacg gcgaggtggt cgagcggaat gactctgccg
     1381 tcgacgctcc ggaagtgatc aaccaatcgc catacgacaa tgcgtggttg atcaaggtaa
     1441 aggtgactga tccggcccag cttgagggtc tgttagatcc cgaagcctac aacgagtatg
     1501 ccgaccaggc ataagtgacg gcctaaggcg tccgggaacc agatccggta tgggtcccgg
     1561 agagttccgt ttgcacgacc catattccgg tcgtgaggtt atcgagggca accaggaaag
     1621 ctatgacgtt caatccccat acggccgaag accgcgaggc aatggtgcag accattggtg
     1681 tcgagagcgt ggaggagctc tttgctcctg tgcccgagcg ggtgcgattc cctcagctcg
     1741 atcttcctcc tgtcctcacg gagatggaag cggcccatcg cctgcaacaa ctcgcagaca
     1801 agaacattgt tcctccagct gaggacatct atctcggtgc aggctcatac cagcattacg
     1861 tgccagccac cgtgggccag atcctgtccc gcggtgagtt ctacactgca tacacgccgt
     1921 accagccgga ggtttcccag ggaaccctgc aggtgatcta cgaatttcag tcgatggtcg
     1981 ccgatctgtt ggggatggaa gtcgccaatg catcgatgta cgacggcgca acggcccttg
     2041 cggagggcgc gttgatgtcc gttgcctcct cgaagaagaa gaacagggtg gtggtggcgg
     2101 gcaccgtcca tccggcatac cgcgaggtga tgcacacgta tcttgacgga tccggagtgg
     2161 aaattgtcga ggctgcgccg aagaacccgt ttgtggtgac cgtggatgac attgcaccac
     2221 atgtgacgga cggtctcgcg tgtgtcgtgg tccagtaccc caacttctat ggcgccatcg
     2281 aggacgtcca ggcgctcgcc gatcatgcgc acggcgctgg tgcgctcttt gtcgtcaaca
     2341 catcgccaat tccgcttggt ttgttgaaag cgccaggagc tcttggcgct gatatcgtcg
     2401 ctgccgaggg ccagcccctt ggcgtagccc agagctatgg cggtccgtat gtcggcctgc
     2461 tgggcagcaa gaagagcctc gtgcgacaga tgccgggccg gattgcaggt gtgacgaccg
     2521 actcggaggg caaacgtggc tttgtactga cgctccagac acgtgagcag cacatccggc
     2581 gtgagaaggc gacgagcaac atctgcacga accaggggct catggcaacc gcggcgacag
     2641 tctacatgtc gagcctcggt cccgagggcc tgcgcgaggt agcgcgggct tgttaccagc
     2701 atgcgcatta cctcgcgaac cgcctgacag agcttgaggg gttcaacctg gcttatgatc
     2761 agccgttctt ccatgagttt gtggtgcaga cacccgggcc agcggcagag gtgaacgctc
     2821 agctgatgga gtccggaatc ctgggcgggc tcgatctggg acagttagac gacgccctct
     2881 ctggccatct gttgctgtgt gcgactgagc tgaactccag ggccgggatt gaccggttca
     2941 ttgatataat cggcagcgat tccaaccggt ttcggtgatc gtcgttccgc aggttgctat
     3001 actctgcggg atgtgaattg tttcgcgtat atatagtgcg cttgtcggtt ttgcggccag
     3061 aacctgcccc gctcgccgtg cgccggatgg aacacctgtg gccaagccaa ttgttgccct
     3121 cgtcggccga ccgaacgtgg gcaaatccac actcttcaac cgattcatcg gcgagcggcg
     3181 agcaatcgtt cagaatgagc caggcaccac ccgtgatcgc acctacggca ccgccgattg
     3241 ggtgggagtc gatttcactg tggttgatac cggcgggctc atggatgacg acgagctcaa
     3301 acccggcact agcgagtccg aggcgaggat agcccgcgaa acacgcgagc aggcgttcgc
     3361 cgcgatcgcc gaagcggatg tcgttgtctt catgacggat gtcacggcgg gtggcccgac
     3421 agccggcgac tatgagatag ccgaaatact gcgacgcgca gataagccaa ccattcttgc
     3481 ggtgaataag gctgacagcg tcaaccggcg ggaagtcgcg tttgagtact acgagttggg
     3541 gctcggcgag cccatggcgg tctcagccta tcacgggaac ggcacaggcg atcttctcga
     3601 ccgtgtggtc gagaatcttc cagagatgga cgaggacgag gacgagctgg agggtcctcg
     3661 aatcgccatc gttggtcgtc cgaacgtggg caagagccgc ctgctcaatg ccttgctcgg
     3721 ccaggaacgg gccatcgtca gcgatgtcgc cggcaccacc cgtgacagcc tcgacaccca
     3781 gatcttctgg gagggacagc cggtcaccct gattgatacc gccggcatca ggcggcgcgg
     3841 acgtgtcgag cacggtatcg agcgcgtgag tgtcatgcgg gcgatgcggg cgatcgatag
     3901 agccgatgtc gtcgttctcg tgatcgacgc ccaggaggac tttacatccc aggacctcca
     3961 tattgccggg tacgttgagg agcaaaagaa gggactcgtt gttgtcgtca acaagtggga
     4021 cctcatcgag aaggaccact caaccatgga cgagtaccga gcgcgtgctg ctcgggaact
     4081 cgacttcatg gcgtacgctc cggttgtctt catctcggcg aagttgggcc agcgcgttgg
     4141 ccaggtgatc gagaaggcat tggaagtgct ggaacagcgg gagaagcgca tcagcaccgc
     4201 cgccctcaat aaggtgttga aggaggcagt ctccaagcat cagccgcctt cccggccggg
     4261 caagtgggtc aaattcttct acgcgaccca ggcggatacc gcgcccccaa cgttcatttt
     4321 tttctgcaac gatcccaagc agatccattt ttcctatcgc cgctatctgg agaacgagct
     4381 gcgccaggcc tttgggttcg agggaacgcc gttgcgactt tccttccgcg atcggcatga
     4441 ggatctctag ccgtggacat cctcaggtca gtgctgctga tcgcgatcac gtatggtctg
     4501 gcgggaatcc cgtggggcgt tgtgctgggt cgtctgatca agggcgtgga cctcaggagc
     4561 tatggaagcg gcgcaactgg tgccacgaac tccctgcggg tgctcggttg gcagatctct
     4621 gtggcggtct ttatcctgga ttttgggaag ggctttctgc cggtgcttct tgcccggtgg
     4681 attgacctcc cgggctgggg aatcgcgctc accgctattg tctcagtcgt cggccactgc
     4741 tggtcgccct atatcggatt ccggggtggg aaggggatgg cgacaggcgg cggtgcggcg
     4801 gtcgcgttgc tgccttggct cttcggattg ctactgttcg tcgtcgctgt cgtctatcta
     4861 acccgctacg tgtctcttgc gtcgatcctg acagccattg tcggcccact catcgtgctc
     4921 gtgctgtggc ttttggggct gtttcccgcc tggtgggtcg tcggcgtcat agggattgga
     4981 tcgatcatcc tgtggcaaca tcggggcaat atcgaccgct tgcggaaggg aacagagcgc
     5041 aagtttggca ggagcgagtc cccctccggc aactctgcct gaccaccatg gtctggcttg
     5101 aaaaagtgga agaccggctc cgggaggaac cggtcttcat tgcgtgtcac ggctaatgat
     5161 gggacttagc tttcataccc gttgggatgt cgctgcatcc agtcccacgc tgagccgatc
     5221 atctcgtcca tcgtcgacct ggtaggattc caatccacca gttcacgggc acgagtcgta
     5281 tcggcgatca acgctggagg atcgcccgcg cggcgctccc cgaaggcaat gggtagttcc
     5341 tggccggtga cccggttgat cgactcgacg atctggcgga ccgaaaaacc atcccgagtg
     5401 ccaagattaa tggcaccgag gggggagtcg aggcgctcga tggctgtaat gtgagcatct
     5461 gcaaggtcgg caacgtggac atagtcacga atcgccgtac cgtccggggt cggatagtct
     5521 gtgccaaaaa cggtgaatga ttcgcgctga cccaagagta cctggagggc aaccggaatg
     5581 atgtgggttt ccgggtcgtg atcttctccc cgttcctccg tggctccggc gacattgaaa
     5641 taccgaaagg cggcgtaatt caggccatac gtctcggcgt atacgggtag cagctgctct
     5701 actatccact tggagtgtcc gtatggattg atcggctcct ttggggagct ctcgcggacc
     5761 gggagggact gcggttcacc gtagacggcg gccgtcgacg agaagacaaa gcggcttatg
     5821 ccggcatccc tggcagcgtt gaggagattg attccgccac cgacgttcgt cgcgtagtag
     5881 tcccccgggt tcgtgaccga ttcgggaacg aggtgcaggg cggcgaaatg aagtatcgcc
     5941 tcggcgccgc ttgttgctac cgcctttgca gtggatccgg catcgcggag atcacaatgg
     6001 agcttgtcgg cgttcggatg caccgctgcc gcatggccgc gggagaagtc gtcgagggca
     6061 acaacatcgt gaccgcgcgc aacgagccgc tcgatcgcga cactgccaat gtatccacca
     6121 ccgcccgtaa ccaggacccg catgtacatc ccccgtctgt gcttgaaggc cggtaaaccg
     6181 ctcacctgag gcgaaacacc cgtagcggat aagaaccacc accatggtac actcaactca
     6241 attttggagt ggacaacttg ttgctttcac tggaagtcca ttgcatgggg atgggcgcat
     6301 ttccacacgg ggtgagacaa ggagaacggt gcagatgata agcatggacg tgcttgccag
     6361 tgcggatcca gaggtggcga gggccgttcg tcaggaggaa atgcgccaga ttcagggcat
     6421 cgagcttatt gcctcggaga actacgtcag cgaggcggtg cttgaggctg taggcaccgt
     6481 gttgacgaac aaatacgcgg aaggtctgcc aggcaagcgc tattacggtg gctgcgaata
     6541 tgtcgatgtt gttgagaaca tcgccattga tcgcgtgaag cagctcttgg gcgcagagca
     6601 tgccaacgtc cagcctcact ctggagccag cgccaaccta gcagcgtttt tctccctact
     6661 cgatccaggc gacaagatcc tcgggatgaa cctttccgag ggtgggcatc tgacccatgg
     6721 gaaggacgtc aatttctctg gtcgcttttt tgaggcccat ttctatggag tggaccagga
     6781 aaccggcctg attgactacg acgacgtttt gaagagagcc agggaagtcc ggccaaaagc
     6841 gattatctct ggcgcaagcg cgtattcgcg aatcatcgac ttccagcgct ttcgtgagat
     6901 agcggacgac gtgggcgcgt atctcttctc ggacatcgcc catatcgccg gccttattgc
     6961 cgccggcgag cacccaacat cagtaggaca tgcgcatatc accacctcca cggcccacaa
     7021 gacgctgcgc ggtccccggt ccggcttcat cctgacggat gaagagctgg gtcctggcgt
     7081 cgacaagacc gtttttcccg gcatgcaggg tggccccttg atgcacgtaa tcgcgggtaa
     7141 ggcggtagcc tttggtgaag ctttgcagcc gtccttcagg gactatgcaa ggcaggtgaa
     7201 ggcaaatgcc gcagccatgg caagagcctt tatagaccac ggtctgccga taatctcagg
     7261 tggcacagac aaccatctct tcctcgttga cgttggctcc aaggggatca gcggtcgcaa
     7321 ggcggagcga attctggact cggtgggcat ctcgacaaat cgaaatacga tccccggaga
     7381 aaccaggagt gcgttccaga cgagtggcct ccgcatcggt acacccgcca tcacaacccg
     7441 gggcttcggc gttgaggaca gcgtccaggt ggcagggttc atctctcgtg tgattgaggc
     7501 gcctgaggat gagtcggtgc tcgcggaagt tcgaagagat gttcttgagc tgactgccgg
     7561 ttttcccgtt cccgggatca gccgctagca gattttgttt cggaccgggt caacaggaaa
     7621 gccatgctac gtgtctaatg ggcttattgc ggcagtcgtt ttcatcgtca cattgatgac
     7681 ttctgtcgca ttcgtctaca agaccaccga tctcttcagc cggctgggcc tggtcgataa
     7741 gccgggcccg cgccgtgttc attccacgcc cgtgccgaga gggcttggtg tcgccatctt
     7801 tctcgcattt cttgtcggtg tcggcgcaac ctattttctt ccaattcctc gtcagcccca
     7861 ggagaccgaa cgcatcctgc tcatggtgat cggcgctgca atagtggtcg gggtgatgct
     7921 ggttgatgac gccataagcc tcggaccggg aacgaagctg acctggcagc tgatagccgc
     7981 ggctgtcgtg attttgcctc gcctgcggtc catcgatcat ggaatggcga tcgaaaacct
     8041 gaatctgccg ttcatcggca cactggccat tcccgtactc atcgcgatcc ctgtgacgtt
     8101 tgtctggttg gtcggcatga tgaatgtaat gaactgggtg gatgggttgg acggccttgc
     8161 tggctccgtc accttggtct catgcgctgt gatgttcgtg catacctatt ggggccccga
     8221 taatgtcccg cagttcacga tctccctgtt accgcttgcc cttggggccg cggtcctggg
     8281 tttcctccca ttcaactggc acccggccaa ggtgatcatg ggcgatgccg gcgcgatgtt
     8341 tcttgggtat gccctcgggg tcatctccat tatcggaggg gcaaagatcg ccacggcgct
     8401 gctggtaatg ggacttccgc tgcttgatgg cttgtgggtc accatgtacc ggatagtcaa
     8461 tggacgctcg cccctgtatg cagacatggg ccacctgcat caccgcctgc tcgctgcggg
     8521 gatggggcag agaaggatcg ttcttctggt ttcggcattt tccgccacct tcggcgcatt
     8581 ggcgctcctt ctccctagcc gcgagcttaa ggcggcaggg cttgtgctga ttgctttcct
     8641 gctgctctca ctcctctggt ggctggcgca caagccgcaa gccagtaaat agagagcgcc
     8701 tgaccttcgg gtcaggcgct ctctatttac tcacgtccgc gtttcccagt tactgaagaa
     8761 ggagctcgaa tttgaggcgc tcttcatcag acccgctacg acggtcaatc aggttataca
     8821 acagtgagat cagttgcaaa aggtactttc cgaagaagac ccccaaaacg actcccccaa
     8881 acaaacccgt agtcgcgccc actatgaacg tgatcggtcc accatgcttg aacaccgggc
     8941 tctgtcctct caagttttct aggtctgcca aacgcgggaa ttcacgtagg tccagcgatt
     9001 cattgaatcg acaggagaac aggagcctcc gaccagcgat cctcaaggtt gtagaaggca
     9061 cgcaagccga cgtcaaagac atgaacgatg acatccccga agtccatgag gatccatccc
     9121 gacgtggtgt caccttcaac ccttagcgga cggatctgcg cctttgccat ctcatccgtg
     9181 attgtccggg agatggcccc gagctggcgc tcattttcac cggagcagat gataaacaca
     9241 tctgcgatcg gtgtgagccc atggatgtcg atcacccggg tatccgcggc tggtgtctcg
     9301 gcgatgatgt cggcaatatc gacggcgagt gcccggctgc tggcaatctc gtccgtcatg
     9361 ctgctttgat gatctgtcac gcgatctctg tttctcctgg gttcccggca ctcaggcgcc
     9421 ggctgagggt ggttacagtg gtaggcccgg cagggatcga acctgcgacc aaggggttat
     9481 gagccccgtg ctctaccgct gagctacagg cccacagaac caacaaccaa ctaccgcctc
     9541 ggtgacgcgg atcgcgtatt catacgatga acagcgccgt cacggttgag cgagatacat
     9601 ccgaccgttg catgcgtcgc tcaagacaag cagtgtacgg atgattatag cctgtcgcaa
     9661 gacggaaggg ttaggggaga tcaatcgcct accgccattc cctgaagctc ccctccgatg
     9721 cgactcaggt cggcctcggg acgttgcttc caggtcagcc ggactccatc gcatagcgtg
     9781 acaaagagga gcgacatgat tgccgcatga gtgagcgttg ccatcagctg caatccgctc
     9841 gctataacga cggcaccgga caaggactgg atcaccacca ggagcatccc caaaactgag
     9901 atcgtgaaaa gatcggggcg cacttcccgg aggcggtaac caagtgtcag caggcccagg
     9961 atgaccagcg agatgatgag tgcgccaatg cggtgaatcg tctggacacc atgaagcccc
    10021 ccgaactctg gaataatttc accgttgcag gtgggccacg aggtgcatgc aagctcagcg
    10081 ccgcggtgtc gcacgtacgc tcccgaatag gcgaccagga tggatataac caccgatgcc
    10141 accgccaatc gtcggaaacg aagcactccg tgatccggga gctggggctt gagcgctccc
    10201 tccccgtggc gtgccttctc agggtgcctg tcagtcagca ctcgagcgag gagcgcggcg
    10261 ctggcgaggc agatcaggga gattccgaaa tgactggcaa gaacctccgg ggtctgtggc
    10321 catttcaccg ccgctgcgcc catcagggac tgcaaaatta gtgtgcccgc cataaccgga
    10381 atcaggatct taacttccgg atagcgccta cgcattggcc aggcgaggac tgatgtgatg
    10441 gcaacgagaa taccctcggc tcctgtcaca atgcgatggc tgtactcgac cagcgactca
    10501 taagtaccgg ctggcaagaa gctgccgtgg cagagcggcc agtcattgcc acagccgtcc
    10561 gcagatccag tggctgtcac acgagcaccc atcaagagga cgatcaacat cccgatcgcc
    10621 gttgcgattg cgaatcccct ggtaatcctg atgcgatcgg tcactgccgc ctgccttaat
    10681 ctcgtcgata acctgtaaga gcgatgcgag ccagaacaag acagtcggct cttttgaccg
    10741 catttcaata gtacgtggtt gcgactcctg gttcatgggc taggttggga agggatgttg
    10801 agatgcagca gtcgctgatg gacgtgaagc gttgcggcga ggagccaagg tcggattcgg
    10861 atctaccttt gatgtacaaa acaaccctac caagggaacc agccggtcct gccttcccag
    10921 ttcttgcctg ccctgaggtg ggcgacgacg gccctctcga gtgacgatcg ttgcggcagg
    10981 gcgtccagat cttccgtcgt tggaccaaac acgaactgaa cgatcgactg gttgacgttg
    11041 tacccgccag gcaggacctg gaacctcccc tgaaaacgac gaatgttcga atcctcacgg
    11101 agatagtcgg ccagctgggg atccagcagc caggagttgc agatgccagc aacgacgggc
    11161 tcgtcgggga agtatgtact gaagaaggta cgtgcctcag ctatcgactc atcgcacgct
    11221 tcgggggtca aaggcccaag gaaatcggga atgtgaatcg acagcacatg gtcgtccgcg
    11281 ctgagcccta gtccaatcct ggacgcttgc tctactgtgc ggtcaccaag tcgggcacgt
    11341 tcaaactgca gccgtcccag ctggtagatc accccgcgga aatggagcgt caaccaccac
    11401 gctacaccga gagcgccctg gcctgtccgc ttgcggtgga cgcggacgtt gcgaccgagg
    11461 tcggcaagcg tcgcctgtac cacactttct gggatactgc gttgtctgaa atactgctgg
    11521 acatgaggta gagcagcaat gaagacgtgg acgtagaaga agcggtattg tggatgattg
    11581 gcgtccatga gcggcgcaaa acgagggggt cccggcacct tccccatgta gagcacgaga
    11641 gaatggaccg ctcgttccag gaaccaccac aattcaggat cggtcttggg agacagcgct
    11701 aatcggagga cgtgcggtac gtcctcctcg ggaacctcaa gatagtgcag cgtattcagg
    11761 agatcctgac cagtgggtag agtgacggta aacggcggag cgccgatttc tacgaggtga
    11821 gtatgccaac gaataaaaac gtcatcctga gtgaagatat cggaactagc cgtcaccatt
    11881 ggtcctgcct attgctgaac ggggagtcac ggttggtcgc gctcctggat tgtacctgac
    11941 agcaattggt ttacgatcag gacaccgcga gggattgcga acttcggctg atcgatcgcc
    12001 ggagcatggg tatcagaaga cagcagggtt tccgaactgt cctgtaatag ccgcggcaag
    12061 gattctgcaa gaggagtgtt gtcggtgttc ccgcttgggg aaacacacgg gctggctgtc
    12121 catggtgttc gttattgcgc gcatcccaat aaaggagaga gtaatgtctc gtatttccag
    12181 aagaacactc atcgcaggtt cggtgctggt gccaattgcc ggaatcggcc tgagaggcgc
    12241 tttggcccag gacaatgaaa gcgacgcgac cccgggagcc tcccctgagg cttcgccgat
    12301 ggcatctccc ggcgcctccc caatggcctc cccggccgct ggtagcaacg aagtcaccgt
    12361 catcgccatc gacattgact ttgagcagag cgaaatgcgt tttcccgcag acaccgacgt
    12421 gacggtaatc ctggagaacc acggcgttct tgagcataat tgggttgttg atgaacttga
    12481 tgccgaaacc gaaacaatcc cgggcggcga aacgaccagc attgtggtga acgcgcctgc
    12541 cggcgagtac gagtactact gctcagtacc tggccacaag gaagctggta tggtgggcac
    12601 gttgatcgtc gagtaagcgc ccaattcgca atctgtggaa tgaaagggtc gcccatgtgg
    12661 cgtctctttc gtcggtcggg tcaccggtgg actggagacc tagcaccgac cgccggagcg
    12721 tcgtatactc attccggcgc acgcgggagt aactcagttg gtagagtgtc tgccttccaa
    12781 gcagaatgtc gccggttcga gtccggtctc ccgctccacg aaaagcccat ggaatcaagg
    12841 aaaaccgccc ctcgacatga agggcggttt tgtgtctggg cctccgtgtg gccgttttgt
    12901 ggccatgacg atgttgaaga gagatgaaag attgacctct ctatcggcaa tacgagtttg
    12961 gggtgccacc ttgcgttacg atagaggcac cgaacaagca accgtccact cacgacgata
    13021 gccgtgaaat cagtattgct cttatcccgg gcacacgagc ttctgacaca tgtcgcaagc
    13081 agttttggtt atcgctcttt aagcggtacg atcggtcctt acacgccaac gcacgtatta
    13141 ctcctccaga ggggtataga attccgtgcc tggtcatcac gcatgttcat tccggtccat
    13201 gccaccagct ggcacggccg atcgagcacg atgtccacca tcacacacga gccggccatg
    13261 accgggcacc agacgcctcc ctgctgcccg ttctccacgc cgatgtcttc cagccaccct
    13321 gtcggccttc ccgctcatca tggagcaagc gtatgcggca ctgaccagag gacaccgtgg
    13381 aacg
//
LOCUS       DASYWM010000307         9294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_410538, whole genome shotgun sequence.
ACCESSION   DASYWM010000307 DASYWM010000000
VERSION     DASYWM010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410538"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1443)
                     /gene="tyrS"
                     /locus_tag="VGR29_12250"
     CDS             complement(91..1443)
                     /gene="tyrS"
                     /locus_tag="VGR29_12250"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV2074399.1"
                     /translation="MSAMIQGAVDDRSIPKTPGGRISAPEYARAEGMNPIDYLRQRGY
                     IHDIVDEEGLKAEFEKGPLTYYIGFDPTGPSLHAGHMATIMLLATLQRFGHRPIALAG
                     GGTAMVGDPTGRTSAREMIRIEELDANVSGISVQLARYLDFEGGRFGGNPAALLLNNA
                     DWLLKLEYIPFLRDIGRHFSVNEMLSTETYRNRLEEGGLNFVEFNYRLVQGYDYLHLY
                     RSENCRLQLGGSDQWSNILGGVDLIRRADGGKVYAVVSPLITTLTGEKMGKTGTGLRV
                     WLNPDMFSPYDYYQYWVNTDDAMVETYLKRFTFIRNEEIEDLTSVQGEALREAKRVLA
                     FEATAMAHGVEEAEKAESATRSLFSGGTPSAGDTDLPTTQIARTEVDGELTLADAFVR
                     AGLAKGRGEARRLAQQGGLSVDDEKVSDVDVVFGDILGERNAVLLRAGKKRFKRVAIV
                     "
     gene            complement(1526..2506)
                     /locus_tag="VGR29_12255"
     CDS             complement(1526..2506)
                     /locus_tag="VGR29_12255"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV2074400.1"
                     /translation="MDLHHPGSFLLEDFSLITHGFLETRLSQRVLALTGAALAGAGAF
                     RISPIFASQDQEIVPANLPVKDVYTFLVAGLDTRTVEEPENTDVMMVSRVDLAAGTVR
                     TMSFPRDLLVEIPGVGYDKINRAYDYGSKADNHDWNAGVALMVETFEHNFGLEIDAVA
                     TTNFQGLPAVVDALGGIRVENPYDVYDAEYPTADYGTKEIFYPAGPLELNGEQALEFG
                     RTRHQDGDDGRVMRQQLILTALLEKAQDPSIISKLPELVEAARDAVKTNIPRDVQAQL
                     IAAVPGIDIEDVHWGVMTQYLWGDTTASGMWVYQGDWSTLPGYVQTWLAG"
     gene            2656..3225
                     /locus_tag="VGR29_12260"
     CDS             2656..3225
                     /locus_tag="VGR29_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV2074401.1"
                     /translation="MTDTTTDITWLTDWPEATRKARTSRKPIIIDVYQDNCGGCVRLE
                     EETLADSTVREQIAERFIPVRMHLFKDRVIAREWQIFWTPTVLFADRSGKVRYSSINY
                     LPVAEFLDVMDIGEAVTAMRWQDYETSIARLRDVEARHPEGPLTAEAIYWRGMAEYFR
                     DGHQSASAKAVWEKIRERFPDSIWAKRQP"
     gene            complement(3753..4262)
                     /locus_tag="VGR29_12265"
     CDS             complement(3753..4262)
                     /locus_tag="VGR29_12265"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEV2074402.1"
                     /translation="MSEYQTRTDVLATIEQVWSEYRLRLAELSSEELEGAIDAAGWTV
                     KDHITHVTAWEASVTAGLNRSPMHEAMGISTSDLNQHIDELNERIREAHAGESVQEAL
                     DASARGHRELILAIEAVPEDVLSMPAGDYMPVRNPDSARQPVHNRILGASAVHYAMHL
                     EAIRKIVSG"
     gene            complement(4284..4586)
                     /locus_tag="VGR29_12270"
     CDS             complement(4284..4586)
                     /locus_tag="VGR29_12270"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV2074403.1"
                     /translation="MTVHSSALLTILLMAAVTYALRAGGYWMMGRVTLSPRMEAGLAY
                     LPGAVITALVVPSAIDAGAPSIVGLMAVAIIMRLWRNLFLALVVGIGTVWLLRQAI"
     gene            complement(4583..5344)
                     /locus_tag="VGR29_12275"
     CDS             complement(4583..5344)
                     /locus_tag="VGR29_12275"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV2074404.1"
                     /translation="MNQTSETLSPPVGITFTQGGVVDGLRATAPLAISVATYGAVFGV
                     LSRQVGVTLVEAMLMNVLVFAGAAQLASLDQWAYPLPIMGIVATVLLVNLRLIMLGAS
                     MRPWLKTLSPRIVYPMLHILSDEAWAVAMTRYRNGARDAGIVLGCNLAIVFAWLPAVA
                     FGHVLGATIGNPAALGLDFAFTAVFGAMLVGGYRSRFDLAPWGASGMVAWIVWWLLPG
                     TWYVIAGGLAGFLVAVLRADQRPHHEALDADGAMP"
     gene            5608..6420
                     /locus_tag="VGR29_12280"
     CDS             5608..6420
                     /locus_tag="VGR29_12280"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEV2074405.1"
                     /translation="MNRERTLSRRSFIRRIAVTGVVAAGATSSLLPFVHGVTAQGGLS
                     VGSSVYVASDDVNLRTGAGLTYSIIRTYSNGATATVVGGPTYASGYSWFNIRKYDGTT
                     GWMAVDFIGGPIDDGGSHGGEWSAGAYVMTSSSLNLRTAPGTNSSIIRTYSGGTTATV
                     LAGPQSATGYQWYKVEVWDDGRIGWFAGEYLETARFEPTGSRRSVVDGPLNVRENPGT
                     SSRILGSVPTGGVVVVRDASFVSQNGYTWANVYVEANSGLISWIAAEFTQET"
     gene            6565..7110
                     /locus_tag="VGR29_12285"
     CDS             6565..7110
                     /locus_tag="VGR29_12285"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV2074406.1"
                     /translation="MSQQDQSHDLNTLLKQHIRDVPNFPEPGIIFRDITTLLADAEMF
                     RLVIDKMKDAYSDTRVDKVVVIESRGFIFGTPIAYGLNAGVVPVRKPGKLPGETYAEE
                     YALEYGTNTLEIHQDAIHDHEHVIIVDDLLATGGTVEATIKLVERSGGEIAGISVLAE
                     LVNLKGRERLGGYPIHSLVTY"
     gene            complement(7453..8691)
                     /locus_tag="VGR29_12290"
     CDS             complement(7453..8691)
                     /locus_tag="VGR29_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV2074407.1"
                     /translation="MSTHDFAFCCMIPKADNDWHGSSPKQGEHAAEREHETVTDGVNE
                     TQIPIFDGHNDTLLSLINTGRDYYQRSEIGHIDDVRAREGGLVGGFFACFVPSAEGQA
                     WSTDVTTLLGSGLKGVPLENEWGGPSQAYAQQFTNAMVARLLNLEVGGGVMLHDLGSE
                     DPPAMSDDPLTIIHFAEDLEENIEAGNFSAILHFEGAEMIDENFYALEAYHAAGLRSL
                     GLVWSRSNQFGHGVPFLAESPDAGPGLTDAGKELVMACNALGIMVDVSHLNEKGFWDV
                     AGTTSAPIVATHSNAHALCRTSRNLTDKQLDAIRESNGVAGVNFNVPFLHPENARDVE
                     MPLTVMADHVDYLVDKLGIDGVALGSDFDGALMPNDLSDCSKLPNLVALLRERGYDDD
                     ALLKIGYRNWVRLLCETWGM"
     gene            8743..>9294
                     /locus_tag="VGR29_12295"
     CDS             8743..>9294
                     /locus_tag="VGR29_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2074408.1"
                     /translation="MAEGTLSGKTAVVMGVQNQWSIAWKIATALADAGANVAITYIDD
                     RAKKNAEQLASTVEGMKTFVCDVQKDEEIAALRESLKSEYGKIDFLVHSIAFAPADEL
                     KNRFLETTREGFKLAHEVSVYSLIAVTRELSPIMDKGGSIMTMTYLGSDRVFPKYNVM
                     GVAKAALESTMRYLAADLGEQGIR"
BASE COUNT         1942 a         2928 c         2685 g         1739 t
ORIGIN      
        1 ggagactgtg agcacagagg cgtggaccgg gatcaattgg ccgaccaagc ccctcgggct
       61 gaactccaag acgctcaaga acggctgata ctacacgatc gccacgcgtt tgaagcgctt
      121 cttcccggcg cgcagaagaa cagcgttgcg ctcacccagg atgtcgccaa acacgacatc
      181 gacatccgaa accttctcat cgtcgaccga gagcccaccc tgttgggcca gccggcgagc
      241 ctctcctcgc cccttggcga ggcccgcccg gacgaatgcg tcggccaagg tcaactcacc
      301 gtcgacctcc gtgcgcgcga tctgcgtggt cggaagatcc gtatcgccag ccgatggtgt
      361 ccccccggag aagagcgacc tggtcgccga ctcagccttt tcagcctcct cgaccccatg
      421 agccatcgcg gtcgcctcaa aagcgaggac gcgcttggcc tcacgtagcg cttcaccctg
      481 aaccgacgtc aggtcctcga tctcctcgtt ccggatgaag gtaaaacgct tgaggtatgt
      541 ctccaccatc gcgtcatcgg tgttcaccca atactggtag taatcgtatg gggagaacat
      601 gtcggggttg agccaaacgc ggagccccgt cccggtcttt cccattttct caccggtgag
      661 cgtcgtgatc agcggtgaga cgaccgcgta gaccttcccg ccgtccgcac ggcggatcaa
      721 gtcgacccca ccgaggatgt tcgaccactg atcggatccg cccaattgca gccggcagtt
      781 ctcagaccgg tagagatgca ggtagtcata cccttgcacc agccggtagt tgaactcaac
      841 gaaattgagc ccgccttcct ccagcctgtt cctgtaagtc tccgtcgaga gcatctcgtt
      901 gacggagaag tgccgcccaa tgtcccgcag gaacgggatg tactccagct tcagcagcca
      961 gtcggcgttg ttcagcaaca aggcagccgg attgccgcca aacctcccgc cttcgaagtc
     1021 gaggtagcgt gcaagctgga cgctgatacc ggagacgttt gcatccagct cctcgatgcg
     1081 gatcatctca cgggccgagg tgcgtccagt gggatcgccc accatggcgg tgccaccacc
     1141 ggccagcgcg atcgggcggt gtccgaagcg ctggagcgtc gcgagcagca tgatggtcgc
     1201 catatgcccc gcgtgcagcg atgggccggt tggatcaaag ccgatgtagt aggtcagcgg
     1261 gcccttctcg aactccgcct tgagaccctc ttcatcgacg atgtcatgga tatatccccg
     1321 ctggcgcaga taatcgatcg ggttcattcc ctcggcacgc gcatactcag gtgcgctgat
     1381 gcgtccgccc ggcgtctttg gaatggatcg atcgtctact gctccctgga tcattgcgct
     1441 catctctcat acacaaaaag ggatgtcacc gggcttcccc ggcgacaccc attgtacgcg
     1501 cttggcccac ctgaacgggg ctttactacc ccgcgagcca ggtctggacg tatccgggaa
     1561 gggtggacca atcaccctgg tagacccaca tcccggaggc agttgtgtcg ccccagagat
     1621 actgcgtcat gacaccccaa tggacatcct cgatgtcgat gcccggcaca gcggcgatca
     1681 gctgcgcttg tacatccctg ggaatattgg tcttcaccgc gtcgcgtgcg gcttcaacca
     1741 gctctggcag tttcgaaata atcgaggggt cctgcgcctt ctcgagcagc gccgtcagga
     1801 tgagctgctg acgcatgacg cggccatcat ccccgtcctg atggcgggtg cggccaaact
     1861 caagggcctg ctcgccgttc agctccagcg gcccggccgg atagaagatc tccttggtgc
     1921 cgtaatcggc cgttggatat tccgcatcgt agacatcgta gggattctcg acacggatgc
     1981 caccgagggc atcgacgacc gcgggcagac cctggaagtt ggtggtcgcg acggcatcga
     2041 tctcaagccc gaagttatgc tcgaatgttt cgaccatcag cgccacgccg gcattccagt
     2101 cgtgattgtc cgccttggag ccgtagtcat aggcgcggtt gatcttgtca tacccaacac
     2161 cggggatctc cacaaggaga tctcgtggga acgacatcgt gcgaacggtg ccagccgcga
     2221 ggtcgacccg tgacaccatc atcacgtcgg tgttctccgg ctcctcaacc gtacgcgtgt
     2281 cgagtccggc cacgagaaag gtgtagacat ccttcacggg cagattagcc ggaacgatct
     2341 cctgatcctg tgaggcaaat attgggctga tgcggaacgc gcctgcgcca gcaagcgctg
     2401 caccagtcag ggcaagtacg cgttgcgaga gtcgtgtctc gaggaaacca tgggttatga
     2461 gcgaaaaatc ctccagaagg aacgaaccag gatgatgcag gtccaagtgt acagcgtacg
     2521 ttcgcgcggc cagtagcgta ttacgccact ggacacgcat cctttctgat acaaacgccg
     2581 tcatcccggc ttcggtttcg gcgtcctccg gcataatacc cagagcagcg atggtttttt
     2641 gcgaaggaca gctccatgac cgacaccaca actgacatta cctggctcac cgactggcca
     2701 gaggcaacca ggaaggcccg gacatcgagg aagccaatca ttatcgatgt gtaccaggat
     2761 aactgcggcg gatgtgtccg gctcgaggaa gaaaccctgg ctgactccac cgtacgcgag
     2821 caaatcgcgg aacgctttat cccggttcga atgcacctgt tcaaggatcg agtgattgcc
     2881 cgcgagtggc agattttctg gacgccgaca gtcctcttcg ccgaccgtag cgggaaggtc
     2941 cggtactcct ccatcaatta ccttccggta gccgagttcc tggacgtcat ggacatcggc
     3001 gaggcggtga cggccatgcg atggcaggat tacgagacgt caattgcccg gctcagggac
     3061 gtggaggcac ggcatccgga gggcccgctg acagccgagg cgatttactg gcgcggcatg
     3121 gcggaatact tccgggatgg gcatcagtct gcgagcgcga aggctgtctg ggaaaagatt
     3181 cgggaacgct tccccgactc aatctgggcc aaacgacagc cgtagcagcg aaagcccggg
     3241 attcatacgg agtagtcgcg ctatcccggc ggactcatag acgcatccgg tgatttcggt
     3301 atcgatgcaa ctggagtccg tggctcgcat gcatagtcga ctactacctg gccgtcaagc
     3361 acccacaatt ggggaccagg tggtgctcaa gatgatcatg ctcatgtgga acgacgagca
     3421 tagatcgggt tgggcagacg aagcggggag gccgcgatcc ggcaccccga aatctctcgt
     3481 cctcatgggt gagtgggaca ggaggggctc tcccacctat acgcacgtcg gttccctcag
     3541 tgcgtatagg tgggagcaga gagcggacga aagctggcgc ccggcatccg tgcgaggaag
     3601 gtgccgtcgt cacgagcgca ccacaccatc gaacatcaat gctccaatcg tatccggcat
     3661 cgatggtgaa ggagcggatg gtcaacaacg gcgggccaac ccgtatgcgt ttccggcagg
     3721 ctccatacac gttgatactg cgacgcgcgt ctctatcccg acacgatctt tcggatagct
     3781 tccaaatgca tcgcataatg caccgcggag gcaccgagaa ttcggttatg gaccggttgc
     3841 ctggcggaat ccggattgcg gaccggcatg tagtcaccgg ctggcataga caagacgtct
     3901 tccggcaccg cttcgattgc caggatcagc tcccggtgcc cccgggcgga ggcatccagg
     3961 gcttcctgca cggactcgcc tgcatgcgct tcgcggatcc gttcgttcaa ctcatcgatg
     4021 tgctgattca aatcagatgt ggagattccc attgcctcgt gcattggact ccgattgagg
     4081 ccagcggtca cggatgcctc ccatgccgtc acatgggtga tgtgatcctt cacggtccaa
     4141 cccgcagcgt cgatcgctcc ctcaagctcc tccgaagaca actccgcaag cctcagccgg
     4201 tactccgacc acacctgttc gatcgtcgcg agcacatccg tcctggtctg gtattccgac
     4261 atcgctccca gcttccacgt tgatcatatt gcctgtcgca gcaaccacac cgtcccgata
     4321 cctaccacca acgccaggaa aagattcctc caaagccgca taatgatcgc caccgccatc
     4381 agtcctacga tactgggcgc gcccgcatcg attgccgatg gcactaccaa cgccgtaatc
     4441 acagcgccgg gcaaataggc cagcccggcc tccatccgag gcgagagtgt cacccggccc
     4501 atcatccagt acccgcccgc ccgcagcgcg taggtcacgg ccgccatcag caggatcgtg
     4561 agcagggcag aggaatgcac cgtcatggca tggccccgtc agcatccagc gcttcgtggt
     4621 gtggacgctg atcggcccgg agcacggcca cgaggaaccc cgcgagtccc cccgcaatga
     4681 cgtaccaggt tcccggcaga agccaccaga caatccaggc caccatcccg gaggcacccc
     4741 acggcgcaag gtcgaaccgg cttcggtagc cgcctacgag catcgcccca aagacggcgg
     4801 tgaaggcaaa atcgaggccc agtgccgcgg gattgccgat tgtcgcgcca aggacatgac
     4861 caaacgcgac cgcaggtaac caggcgaaca cgatcgccag attgcagccc agaacgatac
     4921 cggcatcgcg ggcgccattc cggtatctgg tcatcgcgac cgcccaggct tcatcgctca
     4981 ggatatgcaa catcgggtag acgatccgcg gggacagggt cttcaaccac ggacgcatcg
     5041 aggcgccaag catgatcaac cgcaggttga ccagcagcac agtcgccacg atccccataa
     5101 tcgggagtgg ataggcccac tggtcgaggg aggcaagctg ggcggcgccg gcaaagacaa
     5161 ggacgttcat cagcatggct tccaccagcg tgacccccac ctgccgcgag agcacgccga
     5221 acactgctcc gtaggttgcc acgctgatcg ccaacggtgc cgtggcacgc agaccgtcga
     5281 caacgccgcc ctgggtgaag gttatgccca ctggcggaga caacgtctct gacgtctgat
     5341 tcacagtctc gacaatcact cttcagcttt tctctagcgc gcaaccagtt gatatgctcg
     5401 ggcgtttgat gagcagggac cagccaataa acgaatatcc gcagccagcg tcattatggc
     5461 ggcggccaac cggaagaagg gtacatgcct atgggtcatc gagacacggt actcgctctg
     5521 gccggatctg atgtttccac accatgacgg gaccaaccac actgccaaaa tctcccatcc
     5581 attgcatgac caacgcaggt gagcatcatg aacagggaac gcacactttc ccgacgttct
     5641 tttatccgcc gcatcgcggt gaccggcgtt gtcgccgccg gtgcgacatc gtcactcctg
     5701 cccttcgtcc acggggtgac agcgcagggc gggctctccg tcggctcctc cgtctacgtt
     5761 gcatccgacg acgtcaatct tcggacgggt gccggactga cctacagcat catacgcacc
     5821 tactcgaatg gagccaccgc aacggtcgtc gggggcccga cgtacgccag cggatacagc
     5881 tggttcaaca tcaggaagta cgatggcaca acggggtgga tggcggtgga cttcatcggc
     5941 ggtcctatag atgatggggg atcacatggc ggagaatgga gcgcaggcgc ctatgtcatg
     6001 acctcttcca gcctgaatct ccgtacagcg cctggcacaa attcaagcat catccgcacc
     6061 tacagcggag gaacgacggc caccgtcctc gccggtcccc aatctgcaac tggttaccag
     6121 tggtacaagg ttgaggtctg ggatgatggc cggatcgggt ggtttgcggg cgagtatctc
     6181 gaaactgccc gcttcgaacc gacaggatcg cgtcgaagcg tcgtcgatgg accgctcaac
     6241 gtccgggaga accccggtac gagctcacgc attctcggta gcgtgccgac aggcggcgta
     6301 gttgtcgtcc gcgatgcatc gttcgtctcg cagaacggtt atacctgggc caatgtctat
     6361 gtcgaggcaa actccggtct catcagctgg attgccgcgg agttcaccca ggaaacctaa
     6421 tgccccgatt tccgggccca acagcaacac ccgcgcttgg ttcgtcgagc gcgggtgttg
     6481 ctgttggtgc gcctgccggg ggtacacttc cgggcggtca tcgaacgcga tcgaggactc
     6541 ccactcagcc ctacaggacg atccatgtca cagcaggacc agtcacacga cctgaacacg
     6601 ctgctgaagc agcacatccg tgacgtcccg aatttccccg agcccggcat catattccgc
     6661 gatatcacca ccctgcttgc agatgcggag atgttccgcc tcgtgatcga caagatgaag
     6721 gacgcttaca gcgacacgcg agtcgacaag gtagttgtga tcgagagccg gggcttcatt
     6781 ttcgggacgc cgatcgccta cggtctcaac gccggggtcg tgccggtgcg caagcccggc
     6841 aagttgccgg gggagacgta tgccgaggaa tacgcgctcg agtacggcac gaacaccctc
     6901 gagattcatc aggatgcaat ccatgaccac gagcacgtga tcatcgtcga tgacctcctg
     6961 gccactggcg gcactgtgga ggcaacgatc aagctggtcg agcgctccgg tggagagatt
     7021 gccgggatta gtgtgctggc ggaactagtg aatctcaagg gccgggagcg ccttggaggc
     7081 tacccgattc atagcttggt gacgtactaa cccgatcgga catgatcccg cccgcgttaa
     7141 tgcctgcggc attgcctgtc tggccgaagg ccgaagaacc ctacgcgaag cggttcacgt
     7201 ggacaagaca tctgcgtgag tcatgcgacc tcactcgcta agtccgcgaa gccaagccag
     7261 ttcctcgcca cagcgcttcg cgtagggttc ttcgctccgc tcagaaggac ggcggtttgg
     7321 gagctgcaaa atccttcgga atcacatgtt ggttcccatg acgcaaggtt cgcccctacg
     7381 cggataaaac ctcccgacga cgtcagcaat gccggacgag cacacggcgt ctgtcgtata
     7441 tgtactgctt ccttacatcc cccaggtctc gcagagcagg cgaacccagt tccggtaccc
     7501 gatcttcagc agcgcgtcat cgtcatagcc ccgctcgcgg agcaacgcga cgagattcgg
     7561 caacttgctg cagtcagaca gatcgttggg catcaatgcc ccatcgaagt ccgatcccaa
     7621 tgcgacgccg tcgatcccca gcttgtccac gagataatcc acatggtcgg ccatgaccgt
     7681 cagcggcatc tcgacatccc gcgcattttc cgggtgcagg aacggcacgt tgaagttcac
     7741 gcccgcaacc ccattggact ccctgatcgc gtccagctgc ttgtccgtta gattgcgaga
     7801 tgtccggcag agcgcatggg catttgagtg ggtcgccaca atgggcgccg atgtcgttcc
     7861 ggcaacatcc cagaagccct tctcgttcag gtgagagaca tcgaccatga tgccaagggc
     7921 attgcacgcc atcacgagtt ctttgccggc atccgtgaga cctggtcccg cgtccggtga
     7981 ttcggcaagg aacggcacac catgcccgaa ctggttcgac cgtgaccaga ccaggccaag
     8041 cgatcgcagc ccggcggcgt ggtatgcctc cagcgcgtag aagttctcat cgatcatctc
     8101 cgcgccttcg aaatgcagga tggcagagaa gttcccggcc tcgatgtttt cctcaaggtc
     8161 ctcagcgaaa tggatgatcg tcaagggatc gtccgacatt gccggcgggt cctcgctgcc
     8221 cagatcgtgc agcatcacgc cgccaccaac ctcgaggttg agcagtctcg ccaccatggc
     8281 attggtgaat tgctgggcgt acgcctgcga cggaccaccc cactcattct ccagagggac
     8341 gcctttcagg ccgctgccaa gcagggtcgt cacgtcggta gaccaggcct gcccttcagc
     8401 ggaagggacg aagcaggcga agaacccacc gaccaggcca ccctctcgcg cgcggacatc
     8461 gtcgatgtgc ccgatctccg aacgctggta atagtcacgc cccgtattga tcaggctcag
     8521 caacgtgtca ttgtgcccat cgaagatcgg aatctgcgtc tcgttcaccc catcggtcac
     8581 cgtttcatgc tccctttctg cggcatgctc gccctgcttc gggctgcttc catgccaatc
     8641 gttgtctgcc ttcggtatca tgcagcagaa cgcgaaatcg tgcgtactca tgcgcggggc
     8701 gattctcccg cacacggaca cttcagggaa ggaaaccatc acatggccga aggcacgctt
     8761 tccgggaaaa ccgcagtcgt tatgggggtc cagaatcagt ggagcatcgc ctggaagatt
     8821 gcgacagccc tcgctgacgc aggtgccaat gtggcgatca cctatatcga cgaccgggcc
     8881 aagaagaatg ccgagcaact tgcttcaact gttgagggga tgaagacctt cgtctgcgat
     8941 gtccagaagg atgaggagat tgcggcgctc cgcgagtcgc tgaagagcga atacggcaag
     9001 atcgacttcc tggtgcacag cattgccttc gccccggccg atgagctgaa gaaccgcttc
     9061 ctggagacca cccgtgaagg gttcaagctc gcacacgagg tcagcgtcta ctccctcatc
     9121 gccgttacgc gggagctctc gcccatcatg gataagggcg gcagcatcat gacgatgacc
     9181 tacctcggct ccgaccgcgt cttccccaag tacaacgtga tgggtgtcgc caaggcggcg
     9241 ctggaatcga ccatgcgcta cctcgccgcc gatctcgggg agcagggtat tcgg
//
LOCUS       DASYWM010000308         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_297643, whole genome shotgun sequence.
ACCESSION   DASYWM010000308 DASYWM010000000
VERSION     DASYWM010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297643"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VGR29_12300"
     CDS             complement(<1..581)
                     /locus_tag="VGR29_12300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV2074409.1"
                     /translation="MLPISDDNRGRTRQPIVTWVLIAVNILIYAYQWTLDDRNLLSFL
                     DQWATVPAEVSSGQALFTLVTCAFLHGSWFHLGSNMLFLWDFGDNVEDVMGHAKYIIF
                     YLLTAAGASYAQVLINPDSRVPLVGASGAVSGLLAGYIVLFPHGKIQTLIFLGIFVTT
                     TMIPAWVMIGYWIVIQVISGVGSLSMIDEQTGGV"
     gene            complement(683..838)
                     /locus_tag="VGR29_12305"
     CDS             complement(683..838)
                     /locus_tag="VGR29_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV2074410.1"
                     /translation="MIRNIRTAVKFFVYGMVAGLLFAPRSGDETRKELMNWVSSSVKG
                     VTGGGNT"
     gene            complement(934..1722)
                     /locus_tag="VGR29_12310"
     CDS             complement(934..1722)
                     /locus_tag="VGR29_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074411.1"
                     /translation="MAKKKRRNEASVTVSASARARKTPERSGKGGSGSSKKRHNAAFV
                     VGSVFGGLAGAAAALWKTPYSGEELRSKLTGGGKATNGSSTEGTAVVTTVENREHGQS
                     FKGKVLSIVENKLAPIVGVQLGKTANDDGSVRTTVVDHRVEPTTTSMGPGAGVRPDTR
                     TMPAASSSEEYPGATPAYPDDDPVGYDEDESVGAGAGANVTPVDRDGGEPSRMSSPVT
                     PASEDDAASIEDLTTPQIDLVPEAFKEEEGHMKPFPKLGGNERG"
     gene            1958..>3035
                     /locus_tag="VGR29_12315"
     CDS             1958..>3035
                     /locus_tag="VGR29_12315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV2074412.1"
                     /translation="MPTPDPTIPLQPLGNPVLIEIIREEIRRHGPMTFARFMKTALYH
                     DEHGYYRTPHRKPGRGGDFITSPELHPYFGFTIARQIAECWDRLGQPQQLIVREHGAG
                     IGVLAYDIIAALTQQAPDVRDALDYRLVEVNEYRAAESRAAMDEAGLSHLVRSEHPDE
                     ITPELGIVLANEVADALPVHRLIVRDQELRECWVSLDEEGHFLEEEGVLSQEVAELDV
                     PSYLNEVGVDLAAMPERARLDISPAIAEWACSIAANLIRGYAIVIDYGYDAPTLYRDH
                     RLVGTVRGYFEHTVTDNPYIRVGEQDLTAHVDFTWLTRAATESGMTEIGLTTQSEFLT
                     QLGLGDWLVQLQTEPDTSLEEYYRA"
BASE COUNT          583 a         1034 c          810 g          608 t
ORIGIN      
        1 acaccaccag tctgctcatc aatcatcgac agcgacccca cgcccgagat cacctggatc
       61 acaatccagt agccgatcat gacccatgct ggaatcatcg tcgtggtgac gaatatcccg
      121 aggaagatga gcgtctggat cttgccgtgc gggaacagca cgatatagcc agcgagcaac
      181 cctgagacgg cgccacttgc gccaacgagt ggcaccctgc tgtcggggtt gatcagtacc
      241 tgggcgtaac tggcaccagc cgcggtgagc aggtagaaga tgatgtactt cgcatgcccc
      301 atcacatctt cgacattgtc gccaaagtcc cagaggaaga gcatgttgct gccgagatga
      361 aaccatgacc catgcagaaa ggcacaggtg accagcgtaa acagcgcctg gccggaactg
      421 acctccgccg gcaccgtcgc ccactggtcg aggaaactca ggagatttcg gtcatccagt
      481 gtccactggt aggcatagat gaggatgtta accgcaatca ggacccacgt tacaatgggt
      541 tgtctggttc gcccccgatt gtcatcacta atcggtaaca cggtactgct ctcctcttcg
      601 cccgttcagc atacaggaac gcggccaccc tcaagggcga ccgcgctatc tgcgcatttc
      661 ctgcgtactg ctcgatcctg gattaggtgt tgccgccgcc agtcacaccc ttgaccgagc
      721 tcgacaccca attcatgagt tccttgcggg tctcatcacc gctccgtggg gcaaagagca
      781 atccggcaac catcccgtac acaaagaact tcaccgccgt acgaatattc ctgatcatgt
      841 cattggtcct ttatctcttg aacgaacatc cgtgcgccaa gtggcctatt catccaggta
      901 ctcgcgggaa tcatgccgcg accttgcact gatctagccg cgctcgtttc cacccagctt
      961 agggaagggc ttcatatggc cctcctcctc cttgaacgcc tccggcacga gatcgatctg
     1021 cggcgtcgtc agatcctcga tcgaggccgc atcatcttcg cttgctggcg tcaccgggga
     1081 ggacatccgt gatggctcac cgccatcccg gtcgaccggc gtcacgttcg cgcccgcgcc
     1141 agccccaaca ctttcatcct cgtcgtagcc gacgggatca tcgtccggat acgccggggt
     1201 tgccccagga tactcctccg atgaggaggc tgcgggcatc gttctcgtat ccggccgcac
     1261 accggcaccc gggcccatcg acgttgtcgt cggctccacc cggtgatcga cgaccgtcgt
     1321 cctcaccgag ccatcatcgt tcgcggtctt gcccagctgc actcccacga tcggcgcaag
     1381 cttgttctcg acaatcgaca ggaccttgcc cttgaacgac tgcccgtgct cccgattctc
     1441 gactgtcgtc acgacagcgg tgccctcggt gctggagccg ttcgttgcct tcccaccgcc
     1501 agtgagcttg ctccggagct cctcgccgga atagggtgtc ttccacaggg ctgccgctgc
     1561 accggcaagt ccgccgaata cagagccaac aacaaacgcg gcattgtggc gtttcttgct
     1621 cgacccactc ccccctttgc cggatcgctc cggcgttttc cgcgcgcgtg ccgatgcgct
     1681 tactgtgacg gatgcttcat tacggcgttt cttcttggcc acgttctcat cctcctacgt
     1741 cgacgctgat cggtacgctc cgctgttttc cgggctcccg tcgaaacgcc cgcctgaatc
     1801 ctcaggaaac gagcgccaat atctgcagcc tgttatcaca aatgccatgc catcgtatgt
     1861 gcgagcgcat ggagtcaggg gttcccggac cattccggac catgtgaggt ctacaatacc
     1921 tgtgctacac gagcctcttt gagacgcccg agagacaatg cccacacccg acccgacaat
     1981 accgcttcag ccattgggca atcccgtcct gatcgagatt atccgggaag agatccgccg
     2041 tcatggcccc atgaccttcg cccgcttcat gaagaccgcg ctctaccatg atgagcacgg
     2101 ctattaccgc accccgcacc gcaagccagg ccgtggcggc gacttcatca cctcacctga
     2161 gttgcatccc tacttcggct tcaccatcgc ccgccagatt gccgaatgct gggatcgcct
     2221 tggtcaaccg caacagctaa tcgtccgcga acacggagcg ggtatcggcg tccttgccta
     2281 cgacatcatc gccgccctca cgcaacaggc accggatgtc cgcgacgccc ttgactaccg
     2341 gcttgtcgag gtcaatgagt atcgtgccgc cgaatctcgc gcggcgatgg acgaagccgg
     2401 actgagccac ctggtccggt cagagcatcc cgacgagatt actccggaac tgggcatcgt
     2461 tctcgccaac gaggtcgccg atgcactgcc cgtccatcgc ctgatcgtcc gtgaccagga
     2521 gctccgcgag tgctgggtct cgctggatga ggaaggtcac ttccttgagg aggaaggcgt
     2581 cctctcacag gaggtcgccg agctcgatgt cccctcctac cttaatgagg tcggcgtgga
     2641 tctggccgcc atgccagagc gtgcgcggct ggatatctcc cccgctattg ccgaatgggc
     2701 ttgctccatc gccgccaacc tcatccgggg ttacgcgatc gtgatcgact atgggtacga
     2761 cgctccgacc ctctaccggg atcaccggct ggtggggacc gtccgtggct acttcgaaca
     2821 taccgtgacg gacaacccgt acatccgcgt tggcgagcag gaccttaccg cccacgtcga
     2881 cttcacttgg ctcacccggg ccgcgacgga gtccggcatg accgagatcg gcctgacgac
     2941 ccaatccgaa ttcctcaccc agctcggcct cggcgactgg ctcgtgcagc tgcagaccga
     3001 gccggacacc tcccttgagg agtactaccg ggccc
//
LOCUS       DASYWM010000309         8050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_635338, whole genome shotgun sequence.
ACCESSION   DASYWM010000309 DASYWM010000000
VERSION     DASYWM010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635338"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VGR29_12320"
     CDS             <1..430
                     /locus_tag="VGR29_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074413.1"
                     /translation="GVWPLLKQSIMPITVLALFSSAQLTRFVRASMLETINQDYIRTA
                     RAKGLREQAVIVLHAMRNALIPVITIIALQLPGVIGGAVITEQIFLIPGIGSALIKAL
                     SSKDIPVVMAITFGIAVAVVLFNIVADVLYAIMDPRIKYQ"
     gene            494..1480
                     /locus_tag="VGR29_12325"
     CDS             494..1480
                     /locus_tag="VGR29_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074414.1"
                     /translation="MAENSSPTLLATEQREDRKTLASAEALRVRPQRTLWGNAWRQFR
                     HHRLAMAGLFIFIFFILLCFVGPLVYSELYFSPFKYTTSLTPGSEGRVLGTDSMGQDL
                     LARILFGGRVSLSVGIVAALIAVIVGTLVGAAAGFFGGLTDSILMRITDLFISLPQLP
                     LLLLITFLFKQSMQDWAATVFGAAQVGTFILIVGVIGLLAWMPTARLVRASFLSMKEK
                     EFVEAARSIGAEQNSLIFRHILPNVMSPIIVAATLEVGSAIITESSLSFLGLGFPYDV
                     PTWGAMLFNAKDLLQFSPHEAIIPGTMIFLTVLAINYMGDGLRDALDPRKTT"
     gene            1662..2633
                     /locus_tag="VGR29_12330"
     CDS             1662..2633
                     /locus_tag="VGR29_12330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV2074415.1"
                     /translation="MTHRISRRTLLAGTAATALGTIVSSTLAQPASWNWTMPIGYPGE
                     VLGDGFFMRHGFATENTWFNPGAWHTAEDYYVLDGDAGGAGVYAVADGEAVFAGFDYP
                     GPVIIVRHAEDLYSMYGHLDYDLAVESGPVARGQLLGTVLGRDDGRAPSHLHFEIRRF
                     LITSEVNGPAPRYGFACGPDCAPGPGYWPIDAPEHPAAMGWLNPTHVMATRAFTNGVP
                     EGVEVVVAQGAGERTELRSTPEGEPVGEMPLLPGTRYKLRDIMAGPMDSTETSAEGYQ
                     LWYQIAVPDQANPVWIQAAVPSTHDTGSDGRPSSVRLDLLPLVISEE"
     gene            2805..3725
                     /locus_tag="VGR29_12335"
     CDS             2805..3725
                     /locus_tag="VGR29_12335"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV2074416.1"
                     /translation="MATHRFEPDHYFTTMGAHVPVLHIAPGDTVITTTVDATGQDASK
                     QKVAKTPNAQTGPFFIDGAEKGDTLIVRLERIRPNRDTGFTNALLAPNVVEPEFVHEL
                     QDLPRFDWKIDHDAWTAAPLEPIPGLERLQIPLDPMVGCFGVAARGGQAISTMTSAQH
                     GGNMDYRGFREGVTVYLPIFESGALFSLGDGHAVQGDGEIVGTGIEISMDVQFSVDLR
                     KGESIEWPRGENDHEIFTVGNARPLDQALQHATTEMVRWLQSDFGLSVRAASALLGQC
                     VQYEVGNVYDPAYTMVCKVPKRVLSSLGTH"
     gene            4107..5321
                     /locus_tag="VGR29_12340"
     CDS             4107..5321
                     /locus_tag="VGR29_12340"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2074417.1"
                     /translation="MHSVRVYPAFRQLMLATLATNTAFWMYQVAVGWLALQMTDSPFF
                     VAMAGFVGGIPLLLISLPAGVIIDRFDRRNVLLAAQVGVMVVSAIFALLVSTGLIERW
                     SLLVLVAAYGAIMSFIFPTRTAIVPSLVERRDMANAVALNSASQNATRVVGPSLAGVL
                     IGVLGVAETFAVAAVMQTLALAMTTRLPSLASESTPGAERGLSSLTVGARVVAQRPYL
                     LGLIVLALAPTVLVMPYINLMPVFARDVLGLGSSGLGVLLASTGLGTVAGSLSVARSS
                     GGRGSSSAQIITAAAFAATILAFSLTPIAVAAVVLLFVAGWMSASFLALNQTALQLSV
                     EDEIRGRVLSIYLLTWGMLPFGQLLVGALASQLGTPLAMVVSCLMALASIAAITRRFP
                     SLRQPRAAGSDI"
     gene            complement(5332..5973)
                     /locus_tag="VGR29_12345"
     CDS             complement(5332..5973)
                     /locus_tag="VGR29_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074418.1"
                     /translation="MSAREAREHGHHHWVSITAVLGGLLWIFYGVFEMLQPWGEDTEY
                     RDDLNYEVITDTYLYLLYSVPGSLALILTAVSLVGICRLLSRTSETVARISLVLGYIS
                     VVLGGISLAGVIAGFDPVFTGPRIFGTLALGAATLLAGMRARDNFSLPIRREALVGLG
                     LLGLFLLPLWPLVYAVSVIPEWLGAMIIALFGLGWIAIGYRLWPQHPGVPSPS"
     gene            6376..7365
                     /locus_tag="VGR29_12350"
     CDS             6376..7365
                     /locus_tag="VGR29_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2074419.1"
                     /translation="MSSSTTSVAPEPNSDAIIETRGFSKRYGKFLALDSIDLSIPRAS
                     VGLLGANGAGKSTLIRSLLGIIQPSEGSARVVGYDTRTEGIKVREHVGYMPEADALPM
                     STTAADFVGHMAEMSGLPPRQARQRAADVLYQVGLGEERYRLIKGFSTGMRQRAKLAQ
                     AIVHDPKLVFLDEPTAGMDPKGRDEMLELVERIHKTMGISVVFSSHILEDIERVCDYV
                     VILDGGRLITSQPLGDVNALTGEVALQLESGADQFAEALAAQGVQARRFESETSSYDQ
                     LLIKVEDERVYDLVRDIAVAQHSVIRGMRSQARSLEDIYLGNVGAAREVNRVD"
     gene            7355..>8050
                     /locus_tag="VGR29_12355"
     CDS             7355..>8050
                     /locus_tag="VGR29_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2074420.1"
                     /translation="MSTNPVPTTPEAPQRYGEIFDRGYKHYDGPRRGRSGAITSLIGY
                     SMKRAMGIRKSWTAKVLPFLLYIAVTIPLIVMIGLTAIAPDLEFASYSGYVSGIFFIV
                     GIFVGTAAPEMICVDRHERTLPLYFSRAIDRVDYVVAKVIAMSLLTMTMTVIPSVLLW
                     LGRQLTAEGIGQAMRDNIGDLGRVVFVGVMIALVLSALGLAISSFTNRKGVAITIIII
                     SFLVLTGLANAGLE"
BASE COUNT         1484 a         2422 c         2455 g         1689 t
ORIGIN      
        1 gggagtctgg ccgctcctga aacagtcgat catgcccatc acggtgctgg cactctttag
       61 ctcggcacag ctcacgcgct tcgttcgggc ctcgatgctg gagacaatca accaggacta
      121 catccgtaca gcccgcgcca agggtttgcg ggagcaggcc gtgatcgtcc tccacgcgat
      181 gcgaaatgcg ttgattccag tgattacgat catcgccttg cagctgcccg gcgtcatcgg
      241 cggagcggtc atcaccgagc agatcttcct gatcccggga atcggcagcg cgctcatcaa
      301 ggccctgtcc tccaaggata ttcctgtcgt gatggcgatc accttcggca ttgcggtggc
      361 ggtcgtgctc ttcaatatcg ttgcggatgt cctctacgcg atcatggatc cgcgcatcaa
      421 gtaccaatag tcgtcacagg tcaccggcag aattccagat gcggtgtgtt atcgaccggg
      481 gagataatga ttcatggcag agaactcatc gccaacgctg cttgctacag agcagcgaga
      541 ggaccggaag accctggcaa gtgcagaagc gctgcgggtt cgcccccaac gcacgctctg
      601 gggaaatgcc tggcgacagt ttcgtcatca ccgccttgcc atggcgggcc tgttcatctt
      661 catctttttc atcctgctct gcttcgttgg gccgctcgtc tacagtgagc tctatttctc
      721 tccgttcaaa tacacgacga gcctgacgcc cgggagtgag ggccgggtgc tcggcacgga
      781 ctccatgggc caggacctac tggcgcgtat cctcttcggt gggcgcgtgt cgctctcggt
      841 tggtatcgtc gcggcactga ttgcggtgat cgtaggcaca ctcgtcgggg cagctgccgg
      901 cttcttcggc gggttgaccg acagcatcct catgcgtatc accgaccttt tcatcagcct
      961 gcctcaattg ccgctgctgc tgctcattac gttcctcttc aagcaatcga tgcaggattg
     1021 ggcagcgacc gttttcggtg cggcccaggt tggtaccttc atcctcattg tgggtgtcat
     1081 tggtctgctc gcatggatgc cgacggcgcg tctcgtccgg gcgagtttcc tctcgatgaa
     1141 ggagaaggag ttcgtcgagg ctgcacgatc gattggtgcc gagcagaact cgcttatctt
     1201 caggcatatc ctgccgaacg tgatgagccc gatcatcgtt gcggcgacgc tcgaggtcgg
     1261 ctccgccatc atcaccgagt cgtcactctc cttcctcggc ctcggcttcc cctacgatgt
     1321 gccgacttgg ggcgcgatgc tcttcaacgc gaaggatctc ctgcagttca gcccgcacga
     1381 ggcaatcatt cccggcacca tgatcttcct gacggtactt gcgatcaact acatgggcga
     1441 cggcctgcgg gacgcgctcg atccgcgcaa gacgacctga tcgccgcatt ccacctcgtt
     1501 acagatgaca acgcccatgc tggagtcatc tggcatgggc gtcctgttct gcctttggct
     1561 cagggttacg gcgcgccgaa ggcaagtcag gtggaaaata ctttgggcat ggtggttgcg
     1621 ggagcgcagt aagaacgcac gtacgcggag ggcatcgccc catgacacat cgcatctccc
     1681 ggcgcacgct tctcgccggc acagcggcga cggctttggg caccattgtc tcctcgactc
     1741 ttgcccagcc agcctcctgg aactggacga tgcccatcgg gtatcccggt gaggtgcttg
     1801 gcgacgggtt cttcatgcga cacggtttcg caacggagaa cacctggttc aacccgggtg
     1861 cgtggcatac cgctgaggat tactacgtgc tcgatgggga tgccggtggc gccggtgtct
     1921 atgcggttgc ggatggtgag gcggtctttg ccggcttcga ctacccgggg ccggtcatca
     1981 tcgttcgcca tgctgaagac ctctactcga tgtacgggca cctcgattat gaccttgccg
     2041 ttgaaagcgg tcccgttgcc cggggccagt tgcttggcac cgttctggga cgtgacgacg
     2101 gtcgcgcccc gagccacctc catttcgaga ttcgacgttt tcttatcacc tctgaagtca
     2161 acggtccggc acccagatat ggatttgcct gtgggccgga ctgtgcaccg ggaccgggct
     2221 actggccaat cgatgcaccg gagcaccctg cagcaatggg ttggttgaac ccgacccatg
     2281 tgatggcaac ccgggcattt acgaacggag tgcctgaggg cgttgaggtg gtcgtcgcgc
     2341 agggggctgg ggagcgaacg gagctgagat ctacaccaga aggggagccg gtcggcgaga
     2401 tgccgctact tcctggtacc cggtacaagc tccgggacat catggccgga ccgatggact
     2461 ccacggagac gagcgcagag ggttaccagc tctggtacca gatcgcagtc ccggatcagg
     2521 ccaatccagt gtggatccag gctgccgttc cttccacaca cgacaccggc tccgacgggc
     2581 gtccctcctc ggtccggctc gatctcctgc cgctggtcat ttcggaggaa taggccagac
     2641 tcgggttcgc tctattgctt agctgctgat tctcgcggct gacgatcccg cgcccggaaa
     2701 ctctggaggg gttggcagca tttccccggc tgatatgctc catcatattg atttgacatc
     2761 acccgccgct cggggtaacg gacgcatacg agggagttca ctccatggcc acccatcgtt
     2821 tcgagccaga tcactacttc acgacgatgg gcgctcacgt gcccgtgctg cacatcgcac
     2881 cgggcgacac ggtgatcaca accaccgtgg atgcgactgg gcaggatgcc tcaaagcaga
     2941 aagtagccaa aacgcccaac gcgcagacag ggccgttctt tatcgatggt gcggagaagg
     3001 gtgacaccct catcgtgcgg ctggaacgca tcaggccaaa ccgtgacacc gggtttacca
     3061 atgcgctgct cgcgcccaat gtcgtcgagc cggagttcgt ccacgagttg caggacttgc
     3121 cgcgctttga ctggaagatc gaccatgacg cgtggacggc agcgcccctg gagccgatcc
     3181 ccgggctgga acgtctgcag attccgctcg acccgatggt gggttgcttt ggtgttgccg
     3241 cgcgtggtgg tcaggcaatc tcgacgatga cgtctgcgca gcatggtggc aacatggact
     3301 atcgggggtt ccgggaaggt gtgacggtct acctgcccat cttcgagtct ggcgcgctct
     3361 tttccctggg cgatggtcac gcggtgcaag gggatggcga gatcgttggt accgggatcg
     3421 agatctcgat ggatgtccag ttctcggtcg atctgcgtaa aggcgaatcc atcgagtggc
     3481 cgcgaggcga aaacgaccac gaaatcttca ccgttggcaa cgcccgtccg ctcgaccagg
     3541 cactccagca tgcaacaacc gagatggttc gctggctgca gagtgacttt gggctcagtg
     3601 ttcgagccgc aagtgcactc ctggggcagt gcgtgcagta cgaggtcggg aatgtctacg
     3661 accccgcgta taccatggtc tgcaaagtcc ctaagcgcgt gttgtcctca ctggggacac
     3721 attgatgaat ggtattcagg atgaaggagg ctcccgtgga cgagcaatcg acacgcgata
     3781 atgacagcgg cagcaacact caggaaggaa ggcgcccggg ggatgcagca gatcaggcat
     3841 cgactgggca tgagccagct ccgcaggaag acctggtatg cgcggtatgt gggcacccga
     3901 tcggccagga tgacctcatc tgcccgaact gcggggtgag cctggcagca ggataggatc
     3961 gcggccctcc cgaaagctgc gattgctggc aggtcggggc ggcagtaagg aacgggatcg
     4021 agaccacgaa cgtgtcatcg acttcaagcg aaactcgccg gccttcccaa acgggaccgg
     4081 agcgtgatgg gccgccgggc ggcatcatgc attcggtacg cgtctatcct gcgttccggc
     4141 agctcatgct tgccacgctg gcaaccaaca ctgccttctg gatgtatcag gtcgcggtgg
     4201 gctggctggc gctgcagatg accgactcgc cgttcttcgt ggcgatggcc ggattcgttg
     4261 gcggaatccc ccttcttctg atctccctgc cagcgggtgt aatcatcgat cgctttgacc
     4321 ggcgcaatgt cctcctcgcg gcccaggtcg gcgtcatggt ggtgtcggcg atcttcgcgc
     4381 tcctggtcag caccggcctt attgaacggt ggtccctgct ggtgctggtg gctgcctacg
     4441 gcgcgatcat gtccttcatc ttcccgacac gcacggcaat cgttccttcg ctcgtcgagc
     4501 gtcgcgatat ggcgaatgcc gtggcgctta actcagccag ccagaacgcg accagggtcg
     4561 ttgggccatc gcttgccggc gttctcattg gggtcctggg ggtcgcggag acgttcgcag
     4621 tcgccgcggt gatgcagacg ctggcgcttg cgatgaccac acggctcccc tcactggcct
     4681 cggaatcgac acccggtgcc gagcgagggc tgtccagtct caccgtcggt gcacgagtcg
     4741 ttgcacagag gccctatctg cttggcctga tcgtgctggc actggccccg acagtcctcg
     4801 tgatgcccta tatcaacctt atgcccgtct ttgcgcgtga tgtactcggg ctcggctctt
     4861 caggactggg tgtactgctc gccagtactg gtctcggcac ggtggccggg tcgttgtcgg
     4921 tcgcgcgttc gtcgggtggt agagggagtt cttcggcgca gatcatcacc gccgctgcgt
     4981 ttgccgccac catcctggcg ttttcgctca cgccaattgc ggtggcagcg gtggtgttgc
     5041 tctttgtcgc cggttggatg agcgcctcgt tcctggcgct gaaccagact gcactgcaac
     5101 tcagcgtcga ggatgagatt cgcggtcggg tgctctctat ctacctgttg acgtggggga
     5161 tgctgccatt cgggcaactg cttgtcggcg ccctggcgag ccaactcggg acaccactgg
     5221 cgatggtcgt ctcctgcctc atggcgctcg caagcatcgc agcaattacc cggcggttcc
     5281 catcgttacg tcagcccagg gccgcaggca gcgatatttg agctgcgcca atcacgatgg
     5341 tgaaggcaca ccagggtgct ggggccaaag tcgatagccg attgctatcc atccgaggcc
     5401 aaacagggcg atgatcatcg cgccaagcca ctccgggatg acactaacgg cgtacaccaa
     5461 tggccacaac ggcagcagga agagaccgag caggccaagt ccaacgagtg cttctctccg
     5521 gatcggcagt gagaagttat cacgtgccct cattcccgcc agcagggtgg cagcccctag
     5581 agccagtgtc ccgaagatcc ttggaccagt aaacaccggg tcgaatccgg caatcacgcc
     5641 cgccaggctg atgccgccaa gcaccacaga aatgtatccg agcacgagcg atattcgagc
     5701 cacagtttcg cttgtccggg agaggagcct gcatatcccg accaggctta cggctgtcag
     5761 tatcagggcc aggctgccag gcacactata gaggagatat aggtaagtgt ccgtgatgac
     5821 ctcgtagttc aggtcatccc gatactctgt gtcctcaccc cagggttgga gcatctcaaa
     5881 cacaccatag aaaatccaga gcagcccacc cagcacggcg gtgatactca cccaatgatg
     5941 atgcccgtgt tcacgagctt cccgtgctga catcgacatt cacccttggg ggttgagcgt
     6001 acatagaggg aagacggagt tccttgatga cgtccggtgt tcacaagtat agcggtgaaa
     6061 agcgcgtcat cgggagtgaa tacccgatga aggactgggc ccgaggtgca ggactttcct
     6121 ccataacgat gcgggatgcc agactggttc cggttccatt cagtatctac gacggtcgat
     6181 gcctcacgag gtccgaaacc tatttcccct ctgcaccgtt agacgtacga tgcggcttgt
     6241 tgacacctgc ggtgccatgg cactgatata ctgcgacagc agggaccctc gggccttacc
     6301 ggtctaccgt aatcaagaat cctcggtcaa tactggccgc ggagttgtcc gcctgtgaga
     6361 gaggccgtct cgttcatgtc atcttccacc acatcagtgg ctccggagcc gaattcggat
     6421 gccatcatcg agactcgggg cttttccaag cgctatggca aattcctggc gctcgacagt
     6481 atcgacctgt ccatcccgcg ggcgtcagtc gggctgcttg gcgcgaacgg cgccgggaaa
     6541 tcgacgctga tccgcagcct gctcggaatc atccagcctt cggaaggcag cgctcgtgtc
     6601 gttggatacg acacccggac tgagggcatc aaggtgcgcg agcacgtcgg ctatatgccc
     6661 gaggcagatg ccctgccgat gtccacgacg gcagccgact tcgtcggaca catggcggag
     6721 atgagcggcc tgccaccgcg tcaggcccgg cagcgcgccg cggacgtgct ctatcaggtc
     6781 ggcctcggcg aagagcgcta tcgcctgatc aagggattct cgaccggcat gcggcagcgg
     6841 gcaaagctcg cacaggccat cgtgcacgac ccaaagctcg tttttttgga cgagccgacg
     6901 gcgggcatgg atccgaaggg gcgggacgag atgcttgagc tggtcgagcg gatccacaag
     6961 acgatgggga tttcggtggt cttctcctca catatccttg aggatatcga gcgtgtctgc
     7021 gactacgtcg tcattctgga tggcgggcgg ctgatcactt ctcagcccct cggggatgtc
     7081 aacgccctga ccggtgaagt cgcgctgcag ctcgaaagcg gtgccgatca gttcgccgaa
     7141 gccctggcgg cgcaaggcgt ccaggccagg cgattcgaat ctgaaactag ctcctatgac
     7201 cagctgctga ttaaggtcga ggacgagcgg gtgtatgacc tcgttcgtga tattgccgtt
     7261 gcccagcatt cagtgatccg tggcatgcgt tcccaggcac gatcgcttga ggacatctat
     7321 ctcggtaatg tcggggccgc ccgggaggtg aaccgtgtcg actaatcctg tacccacgac
     7381 accggaggca ccgcagcgat acggtgagat cttcgatcgc ggatacaagc actacgatgg
     7441 cccacggcgt ggccggagcg gagcaatcac ctcgctcatc ggctactcga tgaaacgggc
     7501 gatgggcatt cgcaagagct ggacggccaa ggtccttcca tttctcctct acattgccgt
     7561 cacgatccca ttgatcgtca tgattggctt gacggcgatc gcgcccgatc tcgagttcgc
     7621 gtcctattcc ggctatgtct cgggcatctt ctttatcgtg ggtatcttcg ttggcaccgc
     7681 cgcaccggag atgatctgtg tcgatcggca cgaacgcacg cttccactct atttttcgcg
     7741 cgcgatcgac cgtgtggact acgtggttgc caaggtcatc gcgatgtcct tgctcaccat
     7801 gacgatgacc gtgattccgt cggtcctgct atggctggga cgacagctga ccgccgaagg
     7861 cattggtcaa gccatgcggg acaacattgg tgatctcggt cgggtcgttt tcgtcggtgt
     7921 catgatcgcg ctggtcctca gcgcgcttgg cctcgctatc tcgtccttca cgaaccgcaa
     7981 gggtgtagcg atcacgatca ttatcatcag cttcctggtc ctcaccggcc tggccaatgc
     8041 cgggttggag
//
LOCUS       DASYWM010000310         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_579590, whole genome shotgun sequence.
ACCESSION   DASYWM010000310 DASYWM010000000
VERSION     DASYWM010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579590"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VGR29_12360"
     CDS             complement(<1..1234)
                     /locus_tag="VGR29_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEV2074421.1"
                     /translation="MPEEISQSEERYGALCERALEFVRSRGGVVHEDMLITHVFGNTG
                     TPALWRPLLRNLLSSEERLVFHANGEWGIPSIGPANATGAPLLDEFVALDVETTGLKP
                     SRQRIIEIAAIRYRGGQIERSFEALLNPGRAIPEFISKLTTITNEHVREAPAFDDVAA
                     EVVEFIGDSLIVGHNVGFDIGFMNAELGRAGMPPLINDRLDTMGLAVRLLRSLRKPSL
                     DRVASAVGLSPRKIHRAGGDARLTAEVALRLVAEGARQGVTSLDRLKASATVVAPRPR
                     DDVGRARSILDRSMAHAMPKKPGVYLMKDARGEIIYVGKAKNLRDRVSSYYSQPIGYT
                     RKMDGLIEAVTQIDHEVVGSELEALLLESQLIRRLQPRYNTALKRSEHYPYIKVDTTN
                     PWPRVQVARQWKNDGARYF"
     gene            1377..>2184
                     /locus_tag="VGR29_12365"
     CDS             1377..>2184
                     /locus_tag="VGR29_12365"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV2074422.1"
                     /translation="MADTDQQRHVDELRKRLDRYAYEYHVLDAPTVTDAEYDALFNEL
                     RAVEAAHPELVTPDSPTQQVGGYAPSTFGEIRHPRPMLSLSNVYNEEELTAWATRVER
                     FAGTSDFHLVTEPKVDGLAVALTYVNGRLDHAATRGNGVVGDNITPNIQALRTIPTRL
                     HQPESLPMPGTIEVRGEIYMRRSDFEAFNRRMEEQGGKTFMNPRNGAAGSIRQKDPGI
                     TAQRPLRMFAYQIGYILDGTMPKTHWDALAFLRELGFPTSHEAEHHESISQ"
BASE COUNT          446 a          699 c          612 g          427 t
ORIGIN      
        1 caaaataccg ggcgccatcg tttttccact gccgcgcgac ctgcacccgc ggccagggat
       61 tcgttgtgtc caccttgata taggggtaat gctccgagcg cttgagggcc gtgttgtaac
      121 gtggctggag ccgccggatc agttgcgatt ccagcaggag ggcttcgagt tcgctcccaa
      181 ccacctcatg atcgatctgc gtcaccgcct caatcagccc gtccatcttt cgtgtgtaac
      241 cgatcggttg tgaataatag gacgagacgc ggtcgcggag attctttgcc ttcccgacat
      301 agatgatctc gcctcgtgca tccttcatca ggtagacacc cggcttcttc ggcattgcgt
      361 gcgccatgct tcgatccagg atcgaccggg cacgccccac atcgtcgcgg ggacgcggcg
      421 cgacgacggt tgcagacgct ttcaaccggt cgaggctggt gaccccctgt cgtgctcctt
      481 cagcgactaa tcgcaaggcc acctctgcag tcagccgtgc gtcaccaccc gcccggtgta
      541 tcttccgggg cgatagaccg actgcgctgg ccacacgatc cagactcggc tttctcagtg
      601 atcgaagaag gcgcactgcg aggcccatcg tatcgaggcg gtcattgatc aacggcggca
      661 tgccggcacg gccaagttcg gcattcatga acccgatatc gaacccgaca ttatggccca
      721 cgatcaggga gtccccaatg aactccacca cctcggcggc gacatcatcg aacgctggtg
      781 cctcgcgcac atgctcgttg gtgatcgtgg tcagcttact aatgaactca ggaattgccc
      841 gcccggggtt gaggagggcc tcgaaggatc gctcgatctg accaccacgg tatcggatag
      901 ccgcaatctc gatgatccgt tggcggctgg gtttaagccc cgttgtctcc acgtcaagtg
      961 caacaaactc gtccagcagt ggagcgcccg tcgcgttcgc aggaccgatc gagggaatcc
     1021 cccattctcc attcgcatga aaaaccagtc gctcttccga tgaaagcaag tttcgcagga
     1081 gtggccgcca cagcgccggt gtgccggtgt tcccgaagac atgcgtgatc agcatgtcct
     1141 catgcaccac cccacctctg gagcggacga attccagggc ccgctcacac agcgcgccgt
     1201 accgttcttc gctttgggag atttcttcgg gcatgattac gtcgcaccag cgtgtacccg
     1261 ggcaccattc cgccacatat gtgccagccc gggacattcg aacctttgtg ctacattacc
     1321 agaccggtgg atgaacgtac accggtcaag cccgaattca caggagagcg gcctgaatgg
     1381 cggatacgga tcagcagcga cacgtcgatg agcttcggaa gcggctcgat cgatatgcat
     1441 acgagtacca tgtacttgac gcccctacgg tcacggacgc cgagtacgat gcgctcttca
     1501 acgagctccg cgctgtcgag gcggcgcacc cggaactggt gactcccgat tcacccacac
     1561 agcaggttgg tggctacgct ccatcgacct tcggtgagat caggcatccc cggccgatgc
     1621 tttccctcag caatgtctac aacgaggagg agctgaccgc ctgggcaaca cgcgtagagc
     1681 gcttcgccgg cacttcggac ttccatttgg tgaccgagcc caaggtcgat ggacttgccg
     1741 ttgcgctcac gtacgtcaac ggacgactgg atcacgcagc cacccgcggg aacggcgtgg
     1801 tcggtgacaa catcactccc aatatccagg ctctccggac catcccaacc cggctccatc
     1861 agccggaaag tcttccgatg cccggcacaa tcgaggttcg tggcgagatc tatatgcgtc
     1921 gctccgactt cgaggcgttc aatcgacgga tggaagagca gggcggcaag accttcatga
     1981 atccgcgaaa cggcgcagca ggctcgatcc ggcaaaagga tcctgggatc accgcccagc
     2041 ggccgctccg tatgttcgcc taccagatcg gatacatcct cgacggaacg atgccgaaaa
     2101 cgcactggga tgctcttgct ttcctccgcg aactgggatt tccgacatcg cacgaggccg
     2161 agcatcacga aagcatttcc cagg
//
LOCUS       DASYWM010000311         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_486008, whole genome shotgun sequence.
ACCESSION   DASYWM010000311 DASYWM010000000
VERSION     DASYWM010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486008"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..692)
                     /locus_tag="VGR29_12370"
     CDS             complement(270..692)
                     /locus_tag="VGR29_12370"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV2074423.1"
                     /translation="MMSVALNKNLVRRWIELWNTGDIDAIGGFVTDEYVRHDSNGPEV
                     RGPEAEQQRVAMYLAAFPDLRFTIEHLIAEDNLVLARLTVHGTHQGELMGIPRTGNEI
                     TLTAMELYRLVNGKIAEQWVTIDALGLLQKLGVIPVSG"
     gene            1459..2292
                     /locus_tag="VGR29_12375"
     CDS             1459..2292
                     /locus_tag="VGR29_12375"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2074424.1"
                     /translation="MSTVLPHIPSSRAAHRYQASRSRPRRMSTTYPGILREVGIVSLA
                     IFLYFFVRGLMDSKFSLAAEHAHWLVDLEQRLGIFHEPEVQLWVLEHGWLVWLVNAIY
                     MYGHWPVLIATMVWLIWKRRDQFPVYRSALLISGAIGLVAFVTFPMAPPRFLPELGFV
                     DTVTVHSDAYRVLQPPAFTNQYAAMPSLHVGWNLLMGIALFRTIHHRFWQVLAVVMPL
                     AMYLATILTANHFVLDGVIGSLVALIGLTIAMRISAPVSRRDATPATMSFPCKQRMEA
                     A"
     gene            complement(2606..>2900)
                     /locus_tag="VGR29_12380"
     CDS             complement(2606..>2900)
                     /locus_tag="VGR29_12380"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2074425.1"
                     /translation="QAVRMLSANPAQIAGLHPRKGALRVGNEADLVLLDPHGTWVVHN
                     RDILNEGQWSALNDREITGFVVRTMRRGITIYDADRHDEELLPEGSGALLSRA"
BASE COUNT          572 a          912 c          840 g          576 t
ORIGIN      
        1 gtggcttggg cagcgcacga ccctcacgta gtcttcagcg tcctcgtcgt actgccattc
       61 tatatcccat cccgcaggca gctcttgctc gagccgttcg ggtgatcgca tcgcatggtg
      121 tgagacagcc acttcgtggc cacagacttc gcacgaaacc gttgttgcgt tatccattac
      181 cgaacctcct gactcgacaa caagcggcca gatcatgggg ataaacacag gtgccccgac
      241 aggcgagcaa gaggcaggtg tgctgccacc tatccggaaa ccggaatcac cccaagcttc
      301 tgcaggagcc caagcgcgtc tatcgtcacc cactgttcgg caatcttgcc gttgaccagt
      361 cgatagagct ccatcgcggt cagcgtgatt tcattgccgg tccggggaat acccatcagc
      421 tcaccctgat gcgtgccgtg aaccgtcagg cgtgccagca ccaaattgtc ctcggcaatc
      481 agatgctcga tggtaaagcg caggtcggga aaggctgcca ggtacatcgc aacacgctgc
      541 tgttcggcct cgggaccacg gacctccggg ccattggaat catgccggac atattcgtca
      601 gtcacgaagc caccgatcgc gtcaatatca cccgtgttcc acagctcgat ccaacggcgc
      661 accagattct tgttcaacgc gacggacatc atcagcgctc cttgctccct ggtggggagg
      721 agaatgatat tggcctgccg ggaccccatg ctacatcgtc acgctgaggc gtcaagcaca
      781 ctgaagcttg atctgatgag ccaatgggtc tccgatcgcg ttcccgacag tggcgtgcgc
      841 cgaatcagga gcggcggcat cacgccccgt ggccacgctc gaaacacctc atcacctgcg
      901 acgctttcca gtcacatgcg gggcgctgtg ccgggcagtg aggatcatcg gggaacagga
      961 tcacgagggg caaccaatcc cacccgtcgg acggaggcgc acccgataga gcatcccgag
     1021 ccgcaccagg cgatggagat agctgctcac ccgaacgtgc gagtcgatcc cttgggaaca
     1081 agcatgccga tggtggctgt atgtagtcaa tccgtgtcgc aagacggtgg aattggtgac
     1141 actcgaaaat cccgcgcaaa gccccggata caggcttcca gtgccggggc aggagacgaa
     1201 actgggatcg ggatcacaac cgcactctgg cacgacacgg ctccgccgaa aggtggagct
     1261 atgttcccac catttggtcg gtgtcttgag gatgatgagt cgctataaaa gagttatcgg
     1321 cgccttaccc ccggcgatta gttcgggaaa ggcccacttg ctgggtcggg aactaaagac
     1381 cgaacgtttt cgttaaggtc attaaggttg atgggaccca aaccaggata attcatacct
     1441 ctatccaagg accttgccat gagcaccgtg ctcccgcata ttccctcatc tcgcgcggct
     1501 caccgctatc aggcgtcgcg ttcccgaccc cggcggatgt ccaccacgta tccgggcatt
     1561 ctccgggaag tcgggatcgt ctcgcttgcg atctttctct actttttcgt gcgcgggctg
     1621 atggatagca agttctcgct cgccgcggag cacgcccact ggctggtgga cctcgaacag
     1681 cggctcggta tcttccacga gccggaagta cagctatggg tcctggaaca cggctggctg
     1741 gtgtggctgg tcaatgccat ttatatgtat ggtcactggc cggtgctcat cgcgaccatg
     1801 gtgtggttga tctggaagcg gcgggaccaa ttcccggtct atcgctccgc cctgctgatc
     1861 tcgggcgcga tcgggttggt tgccttcgtg accttcccca tggcgccgcc ccgattcctg
     1921 ccggagctgg gattcgttga tacggtcacg gtacattccg acgcctatcg ggtcctgcaa
     1981 ccaccggcct ttacgaacca gtacgccgcg atgccaagcc tccatgtcgg ctggaacctg
     2041 ttgatgggaa tcgcgctctt ccggacaatc caccaccgtt tctggcaagt gcttgcggtc
     2101 gtcatgccgc tggccatgta tctggcgacg atcctgaccg ccaatcactt tgttctggac
     2161 ggcgtcattg gctccctggt cgcccttatc ggtctcacga tcgccatgcg aatcagcgct
     2221 ccagtctcca gaagggacgc cactccagca acgatgagct ttccttgcaa gcaaaggatg
     2281 gaagctgcct gacgctggac ccctccgtca ttcagagcgc agcaaaggct cgctgcggcc
     2341 cggccaagcc cgcaagggga gaagcggagc gagccttttg ggccctgaag gctcggccat
     2401 gagaatcctg cgcgaagcgg tgtaggcgag ccggcatgag gcttcctgga aacgaccggg
     2461 tcatcctgca caaacccttg cttcccgcca tggacggcgt gccaatcccc tgccactgct
     2521 gcagaggatt gacacgccgt ccatcacgcg gccctggccg ggtcgccgaa caaacctcat
     2581 gaagaccgct ggtactgctc aagcctcatg cccgcgagag cagggcaccg cttccctcgg
     2641 ggagtagctc ttcgtcgtga cgatcggcat cgtagatggt aatgccgcga cgcatggtgc
     2701 ggacgacgaa gcccgtgatc tcccgatcat tgagagcgga ccactgaccc tcgttgagga
     2761 tgtcgcggtt gtgcaccacc cacgttccgt gagggtcgag gagcacgaga tcggcctcat
     2821 tgcccacccg taacgccccc ttgcgtggat gcagcccggc gatctgcgcg gggttggccg
     2881 agagcatccg taccgcctgc
//
LOCUS       DASYWM010000312         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_579735, whole genome shotgun sequence.
ACCESSION   DASYWM010000312 DASYWM010000000
VERSION     DASYWM010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579735"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VGR29_12385"
     CDS             <1..237
                     /locus_tag="VGR29_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEV2074426.1"
                     /translation="RSMQTEKNDQKIGYNVLTDEYVNMIDAGITDPVKVTRSAVANAT
                     SIAGMILTTEALISDKPEKEMAGMPPGGMGGMDY"
     gene            complement(406..711)
                     /locus_tag="VGR29_12390"
     CDS             complement(406..711)
                     /locus_tag="VGR29_12390"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV2074427.1"
                     /translation="MSNKPGLKVIIDPHRCIANAKCTTAAPGIFVLDEETGVAVLEDF
                     EGATVEQIFAGARACPTQAIQIEQYGHRVFPQILTPMWGDSRPSDGHDDNDGPSSKM"
     gene            complement(765..1424)
                     /locus_tag="VGR29_12395"
     CDS             complement(765..1424)
                     /locus_tag="VGR29_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV2074428.1"
                     /translation="MDIPYRIIASDGHHEIEIRKSRFICSVFRASTEEEARSHIDSIR
                     KQYWDASHNCTAWALGPDLKLQRSSDDGEPSGTAGIPMLEVLRRRQITDTVAVVTRYF
                     GGTMLGAGGLIRAYGGAVTGAIDAIGVVERKRLSVQTLVAPYDDAGRIENAIRSSEYP
                     LGDVSYGEKVTFELVMEPELVQPFRHWLAEMTNGTIVPLDSGERFVDVRAPTEAQHQP
                     K"
BASE COUNT          346 a          512 c          475 g          345 t
ORIGIN      
        1 cgctcgatgc agaccgagaa gaacgaccag aagatcggtt acaacgtgct caccgacgag
       61 tacgtgaaca tgatcgatgc cggcatcact gatccggtga aggtcacccg ctccgcggtg
      121 gcgaatgcca cgtcgatcgc cggtatgatc ctgacgaccg aggccctcat ctccgacaag
      181 ccggagaagg aaatggccgg catgccgcca ggtggcatgg gcggaatgga ctactaggag
      241 aaacatcacc ggagcaggat ccgttcccgt tccggtgatc tcaaaggttg acacaaaagg
      301 agggggaagg catctagcct tccccctcct tttgtgtcaa cgtcgggacc tgctgccctg
      361 caacccctga cgatccaagg gatgtcgatg gggcgcgagc gtcgatcaca tcttcgacga
      421 gggcccgtcg ttgtcgtcgt gaccgtcaga cggccgcgag tcgccccaca tcggagtgag
      481 gatctgcggg aagacccgat gtccatattg ctctatctgg attgcctgag tcgggcatgc
      541 ccgtgcgcca gcaaagattt gctcaacagt cgccccctcg aaatcctcga gaaccgcgac
      601 gcctgtctcc tcatccagca cgaagatgcc aggagcggca gtcgtgcatt tcgcattcgc
      661 gatgcagcga tgtgggtcta tgatcacctt cagtccgggc ttgtttgaca tcgttattct
      721 cctctgcgtc ctctcgacgc gactggttat catcaacttt accgctattt tggctgatgt
      781 tgggcttcag ttggtgcacg cacgtccaca aatcgttcgc cggaatccaa cggaacaatg
      841 gttccattcg tcatttcggc cagccagtgg cggaagggct ggaccagctc cggttccatg
      901 accagctcga aggtgacttt ctcgccgtac gagacatccc ccagcggata ctcgctcgac
      961 cggattgcat tctcgatccg gcccgcgtca tcgtagggag cgacgagcgt ctgaaccgaa
     1021 agccgcttcc tctccaccac cccgatagcg tcgatcgcac ctgtaactgc cccaccgtag
     1081 gcccggatca acccgccagc acccagcatg gtgcccccga aatagcgagt gacgaccgcg
     1141 accgtatccg taatctgcct gcgcctaagg acctcaagca tcgggatgcc cgctgtacct
     1201 gatggctcgc catcatcgct ggaacgctgg agcttgaggt cagggccaag tgcccaggcc
     1261 gtgcaattat ggctggcgtc ccaatattgc ttgcggatgg aatcgatatg cgagcgggct
     1321 tcttcctccg tggaggcccg gaacaccgag caaataaacc gggacttcct aatttcgatc
     1381 tcgtggtgcc cgtcgctggc aatgatgcgg tacgggatgt ccatggtcct cccattagcg
     1441 actgcggtct caaggttcag ccgttatgca ggtacattct acgcggcaca ctcgcgaata
     1501 taggtggcgg acagtcctgt ttcgcgatct gtcggtcctg ggaatcctgt gtggccggca
     1561 ttgtgcaaac tggcataatt ccggaagcga ccatcgctcg ccgcattgag cgaaagcacc
     1621 cacgagctgc tttcgcggca cattgaccgg catcatggct gcgaaaagag ccagatct
//
LOCUS       DASYWM010000313        10000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_148216, whole genome shotgun sequence.
ACCESSION   DASYWM010000313 DASYWM010000000
VERSION     DASYWM010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10000
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148216"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1166..3421
                     /locus_tag="VGR29_12400"
     CDS             1166..3421
                     /locus_tag="VGR29_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV2074429.1"
                     /translation="MATHTRAQGSTKKNSRSRSSRKSQATGLSLWALRGREFLSGLIP
                     KVAVSERLQREMLGVVLVVLALISSWALGRGQEDGALIAWWGKVLRVTLGNAAFIVPV
                     FAALTAVRAYAQSSGPILLFRHYVGGMLFLLAVTGLMSFGGENYGGNVGDVAGDIARG
                     ALGAVASGLVFFAAGVVSVFLLADTDLQTLDRDVRRLLPERKPRPSKTNESPDTAKKA
                     GQAGKEVKPVVRRAAPKLESARPEYVPPVINLPAKPEKGPVTEAALAQPLPLPAEVAS
                     NEKPLPLPDLRQLPYYEAVVPDPADLEAKAKTIEATLQSFKVEASVREINPGPAVTQF
                     ALEPSSGVKVRRITELQNDLALALAAHSVRVEAPIPGMARVGIEIPNEKIATVGLREV
                     LESPAFTKSKAKLPLPLGRDVNGRYIVGDLTKMPHLLIAGATGSGKSVCINGIISTLI
                     SIKQPDELRLLMIDPKMVELTGYNGVPHLQCPVVTEMDKVVSALRMVLREMNRRYELF
                     KQVGVRNIDGYRMRMVDEPDAEKLPYLVTIIDELADLMMTTPDEVETLLARLTQMARA
                     TGIHLIIATQRPSVNVLTGLIKANVPARIAFAVSSMIDSRVVLDMPGAERLLGRGDML
                     YLPPDEAKPTRIQGAFIDDDVHSIVDHWHTVSPIPQYAPEWMDLPSSKESKGGYGGDT
                     EDDGEEEPYMQQALDIVRAQGTASASMLQRRLRIGYNRAARIIEQMEDDGVIGPADGA
                     KGRPVLVSFEE"
     gene            complement(3507..3827)
                     /locus_tag="VGR29_12405"
     CDS             complement(3507..3827)
                     /locus_tag="VGR29_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEV2074430.1"
                     /translation="MEFSDKTLTCRECSKPFVWTAGEQAFYKDRQLINIPARCTECRS
                     NRKERLGLQRQPITEVVCAECGLTTTVPFVPRNGRPVYCSTCLAGVRAQEESRQPSTA
                     ATSV"
     gene            4095..4706
                     /gene="recR"
                     /locus_tag="VGR29_12410"
     CDS             4095..4706
                     /gene="recR"
                     /locus_tag="VGR29_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEV2074431.1"
                     /translation="MTSRYGSATAPPVAQLIEELSKLPGIGPKTASRLAFHLLRTSRD
                     DALALAEAILEVKERIRLCSNCFNVTESDPCDICADQKREHVICVVEKPLDIVAIERT
                     NEYRGKYHVLHGAISPVDGIGPDKLKIRELVERVEHEKPEEIVVFTNLDLPGDATATY
                     LHRILTPLGVNVSRPASGLPVGGDLEYADEMTLGRAMLGRRVL"
     gene            4879..6033
                     /locus_tag="VGR29_12415"
     CDS             4879..6033
                     /locus_tag="VGR29_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074432.1"
                     /translation="MHDQAPYRQVPSEDVSPATDHGTPSVRIEVRRAGFTDLLALSRF
                     SSRYQLNQPGSSHTDSDPARATLRGYLPFSREDQPVFVATNEEERRLLGFVQFRVAGP
                     DQRWIAESIGANAGVYDPEPVVLELVRHAIKAAGLSGVKRLYARLEAECPIEQPMRQI
                     GFTPYGRELVMASPSVPVLPAARGVRIQEQADVWAIHQLYLQSTPRDVQYAEALTSHS
                     WDVDAVLRSRGYGCRGWLIADHHLAVAYARAITRRDAHIVDLMVLPDHRDVLPALLSS
                     VFRELATLPSRLVYVVVRHYQSEIVVVLEDLGFVAQIEQEVHIRYTTASVRSSVMAAH
                     YSVADSQKEPAVKRVPTFFQGTADRYYMQEGVLAPEDRRNEAPRAFETGG"
     gene            6079..7938
                     /locus_tag="VGR29_12420"
     CDS             6079..7938
                     /locus_tag="VGR29_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEV2074433.1"
                     /translation="MSSFPQALPDEHTRSAAETEVTDDIRELLEVLPEAISRRVIQLQ
                     DNHESGELIEIVLDLGRPPEARFQHDEIDLGDLDVSRADLGHVAERIGQFGDDNRAGI
                     ERTLHRISAIRNRSGEIVGLTCRIGRAVYGTIAIISDLIESGQSVLLLGRPGVGKTTL
                     LRETARVLADELRKRVIVVDTSNEIAGDGDIPHPAIGRARRMQVPTPTAQHAVMIEAV
                     ENHMPQVVVIDEIGTELEAMAARTIAERGVQLIGTAHGNSLENLMLNPTLTDLIGGIQ
                     SVTLSDEEARRRGTQKSILERKAPPTFGVMVELVDRDEVVVHLDVAATVDALLRGAPV
                     RSEHRHRKLDGTIETSSSEDVRRDPSSPRRGPSVVNPSSRNAPRTTNDRGERNFADIF
                     ASGEPRDEERSTELLSRGHRHLTAIDTTEATVQPRAAAPAVTAFAKGRGTDDKPLRIF
                     PFGVSRNRLDQSISRLKVPAVIVRTERDADIVLTLKNAYRQKAQPVRDAEIRGIPVYV
                     LRSNTNTQMENVLMSIFPQARIEEQEDDGDGGDEPAPSRRERRTNDVVIAMSEAEEVI
                     SAVMQGAPATALRPQAPPIRRMQHEMAERYNLASRSRGRDPFRHVEIYRRGMQ"
     gene            7939..8595
                     /gene="tmk"
                     /locus_tag="VGR29_12425"
     CDS             7939..8595
                     /gene="tmk"
                     /locus_tag="VGR29_12425"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV2074434.1"
                     /translation="MRGPFITLEGPEGAGKSVQAAFLASFLEEQGWDVLSTREPGGTG
                     FGDALREILLGTDGVAILPEAEVLLLAAGRAQHVRERILPALDAGVAVVCDRFVDSTL
                     AYQGGGHGIPLEQLLPIQKFATGGLEPDLRILLDVPVDIGLRRRHADPSLVNRIDLAS
                     REFHERVRATFLQLAKANPEGWSIIDGREDVASVARKMHTDVQRRVLARFAVPNPGVV
                     "
     gene            8620..8958
                     /locus_tag="VGR29_12430"
     CDS             8620..8958
                     /locus_tag="VGR29_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEV2074435.1"
                     /translation="MKLVIAVVQGKDAEPLLEALTTHGFRATQINSAGGFLRESNATL
                     MIGVDDGEVERVRSLVQENCHSRTRFVNPLMPIADVAEFYSSSPVEVLVGGATLFVLS
                     VNRYERLEAS"
     gene            8995..9795
                     /locus_tag="VGR29_12435"
     CDS             8995..9795
                     /locus_tag="VGR29_12435"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2074436.1"
                     /translation="MEIDSFIARALQILLALGGAYLLATWFVLAIWTFRDIEARSRNA
                     VTQIFSTLLAVFFWIPGVLLYMLLRPKETLDEAYQRSLEEEYLLQDLQELPLCPTCNH
                     FVHDDWQLCPHCNTQLREPCGNCRHLVDLRWDICPYCGQEQEAHVPHVHEKKADGGKH
                     GWIDPAIIEKRLRDEEARRQREAPRALPAATAIDRDLLSPAAQNLTQETTVITARPSQ
                     DGRAPLTQVGKSPVGPDITDLRGDGSNGTEEKVGEPVKIPRKFRINAD"
BASE COUNT         2007 a         2854 c         3109 g         2030 t
ORIGIN      
        1 cccaccgccg cagtgcttca gggcatcgta gaggatgtgc tcatcccctg gaggcgacgc
       61 cacttggctc cagtggaggc gttagcaaca cattccaccg acctggacag ttgagagagg
      121 cccgcagcct acattacggc ttacagcagt ttgccacgag attgataggc cggggcgggt
      181 gaaggtggta gccaggggct cactgtcgca gcaatagcct gccctgagcc tgtcgaatgg
      241 gtcatgtgca acctgtcagg aggacagtgc tgcgtcgtgg tcgcgaaggg gccgcatgca
      301 ccgcaagtcc gccgcgacgg acgtgtaacc ttcctgcaaa tgctttagat ggccaagtac
      361 ataacctggc cggtggcagc ctaaaggttc ctgcaacccg atgtccttga cgtgacaggg
      421 gtttctttag atggccacgg tgtgtgcagg gcgggcgagc acctggccag gtcagacgcg
      481 gtgggaggac gaaggctgcc acgtatttca tgtggcagtg tggcggcctt ggcccgtgtg
      541 agggtcattc tccccaatcc atggggtgtt caggtctgcc atatcgctcg atactcgttg
      601 gcagccttga cgaggtcggg caggtgtcac cagcgagatg gtacagacgc tctcccggtg
      661 atacgcgtcc aggaagagga tctgcgccgt tatctcgccc ggatcagatt gaagcggtgt
      721 gcgtgaccat tgaccggatg cctgcgtcgg ggagcaccac ataggcgaat ccaaaggatt
      781 cgatgggccg ctgtcgctca atacctgtgg acttcctgtt tgcgaatgcg gcccattgct
      841 gtcctccctt gtcagggggc gggaggacat ggagtcctcc cctacgggat ggggaagccc
      901 catccttcct gcaaaccgat ctaggtgatg tgagaccgcc gaacaaatta gtccgcagaa
      961 gccatctggc gagtgagcgt gtcctgccca ccaggaatcc caagccgcaa ccgttcctat
     1021 caccctttgc gccatccctc gaccattttg actccgtggc atataatgta cgtacagtca
     1081 tgcagacgct ggacgcaact cacggttcct gagagccgtg ctgatgcggt gaaggaaaat
     1141 acgttcgttt tggagtagcg ggccgatggc gacccacacc cgggcacagg gatccaccaa
     1201 aaagaattcc aggagccggt cctcgagaaa gtcgcaggca accggacttt cgttgtgggc
     1261 cctgcgtgga cgtgagttcc tgagcggtct gatccccaag gttgcagttt cagaacgcct
     1321 tcagcgagaa atgctaggtg ttgtccttgt ggtccttgcc ctaatcagct cgtgggcact
     1381 cgggagaggt caggaagacg gagcccttat tgcctggtgg gggaaggtcc tccgagtaac
     1441 gcttggcaat gcggcgttca ttgttcctgt cttcgctgcg ctaacggccg tgcgcgctta
     1501 cgcacagagt tccgggccga tcctcctgtt ccgccactat gtcggcggga tgcttttcct
     1561 gttagctgtc acgggattga tgtcgttcgg cggcgagaac tacgggggca acgttgggga
     1621 tgtcgctggc gatatcgcac gtggagcgct tggtgcggtg gcatcgggcc tggtgttttt
     1681 tgccgctggc gttgtctccg tcttcctcct tgctgatacc gatcttcaga cgcttgaccg
     1741 ggatgttcga cgcttgctgc cggagcgaaa gccacgcccg tcgaaaacga atgagtcgcc
     1801 cgacactgcg aagaaggctg gtcaggcagg gaaggaggtc aagccggttg tccggagggc
     1861 cgccccaaag cttgagtccg cacgcccgga atatgtaccg ccggtcatca atctccccgc
     1921 taaaccggag aaagggccgg tgactgaagc ggctcttgcg caacctctgc cgttgcccgc
     1981 cgaagttgct tccaatgaga agcctctgcc actgccggat ttacgccaac tgccttacta
     2041 cgaagcagtc gtgccggatc ccgccgatct tgaggcgaag gcaaagacca tagaggcaac
     2101 cttgcagagc ttcaaggtgg aggcatcggt tcgcgagatt aacccgggac cggcggtgac
     2161 gcagtttgcg ttggagccaa gcagcggtgt caaggtgcgc cgaatcacag agcttcagaa
     2221 cgatttggct cttgcgctcg ccgcacatag cgtccgggta gaggcgccaa ttcccggcat
     2281 ggcgcgtgtg ggtattgaga tcccaaatga gaagatcgcg acggtcggtc ttcgcgaggt
     2341 gctggaatcc ccggcgttta ccaagagcaa ggcgaagttg cccttgccgc tgggccgaga
     2401 cgtcaacggg cgttacatcg tcggcgacct gacgaagatg ccgcatctcc ttattgcagg
     2461 tgcaaccggc tccggaaaga gtgtgtgtat caacggcatc atctcaacgc ttatcagtat
     2521 caagcagcct gatgagcttc ggctcctgat gatcgatccg aaaatggtcg agcttaccgg
     2581 gtacaatggc gtgccgcacc tccagtgtcc cgttgtaacg gaaatggaca aggttgtcag
     2641 cgccttgcgc atggtgctgc gggagatgaa ccgacgctac gagctcttca aacaggtagg
     2701 cgtgcgcaac atcgatggct accggatgcg gatggtggac gagcctgatg cggagaagct
     2761 tccctatctg gtgacgatca tcgatgagct cgcggacctg atgatgacaa ctcccgacga
     2821 ggtcgaaacc ttgcttgccc ggctgacgca gatggcccgc gcgacgggaa ttcacctgat
     2881 catcgcgacg cagcgacctt cggtgaacgt gctgacgggt ttgatcaagg cgaatgtgcc
     2941 cgcccggatc gcgtttgcgg tttcttccat gatcgatagc cgggtcgtgc tggatatgcc
     3001 tggagccgag cgattgttgg gacgcggcga catgctttac ctgccgccag acgaggccaa
     3061 gccgacacgc atccagggag cgtttatcga cgatgacgtg cactccatcg ttgatcattg
     3121 gcatactgtc tcacccattc cccaatacgc cccggaatgg atggacctgc cgagctcgaa
     3181 ggagtccaag ggcggctatg gcggtgacac cgaagacgat ggcgaggaag agccatacat
     3241 gcaacaggcg ctcgatatcg tgcgggcgca gggaacggcg tcagcctcga tgctgcagcg
     3301 tcgtcttcgg atcggctaca accgggctgc gaggattatc gagcagatgg aagacgacgg
     3361 ggtgatcgga cccgcggatg gggcaaaggg acgaccggtc ctggtcagct tcgaggaata
     3421 gtcaccatgc ccctggccct tcggccagac ttggaacgcc cgcgacgaaa ttcgtcgcgg
     3481 gcgtttgttt cactggaagt cgccccttat acggacgtgg cagcggtgga gggttgcctg
     3541 gattcttcct gtgctcgcac acccgcaagg cacgtcgagc aatagaccgg cctgccgttt
     3601 ctggggacga acggaacggt tgtcgtcagc ccgcactccg cgcagacgac ttcggtgatc
     3661 ggctgccgtt gcaggcccag tcgttcctta cgattcgacc ggcactcggt gcagcgcgcc
     3721 gggatgttaa tgagttgacg atccttgtaa aaggcttgct caccggccgt ccacacgaat
     3781 ggcttgctgc actcgcggca ggtcaacgtt ttgtcactga attccatctc tggcctccga
     3841 gtcccgtaac gaatctatcg tttcaaggcc gcgcttcgtt gaagcccgat cctttgatgc
     3901 agaccctggc gctcagcgcc atacggctat gtcgccatcc atacggacac ccagtgaggg
     3961 tgagacgaaa ctgtttgagc gaccgatcgc cgaatacgtg attcccacgg ccagcatatc
     4021 agaaaaatct agaaagtgat acacttcaaa tgacatctgt gagaacagat gttccataaa
     4081 taacgaggaa cttaatgaca tcacgatatg gctccgcgac ggctccgccc gttgcccaac
     4141 tcattgaaga gctttccaag ttgccgggaa tcggcccaaa gactgcttct cggttggcct
     4201 ttcatttatt gcgcacatcg cgcgacgatg cgttggcgct cgctgaggcc atccttgaag
     4261 tcaaggagcg aatccggctg tgctcgaatt gcttcaacgt cacggagtcc gatccttgcg
     4321 acatttgtgc tgaccagaaa cgagagcacg tcatctgcgt ggtcgagaaa ccactggaca
     4381 ttgttgcaat cgaacgcaca aatgagtacc gcggcaaata tcacgtgctc catggagcga
     4441 tctcccctgt cgatggtatt ggcccagaca agctgaagat tcgcgagctg gtggagcggg
     4501 tagagcacga gaagcccgaa gagattgtgg tcttcaccaa ccttgaccta cctggcgatg
     4561 cgaccgcaac ctacctgcac cggatcttga cgcccctagg tgtgaatgtt tcccgtcccg
     4621 cgagtggatt gccggtcggt ggcgaccttg aatacgcgga cgagatgact ctcggtcggg
     4681 cgatgctggg ccggcgcgtt ctttaatcgt cccggcccgg attggctcgg gaattgggct
     4741 ctctgggggt tgagtcctgt agcgagaatc gctagaatca agtcaatgga taatttgggt
     4801 cgtcaccccg cacgtgacgt tctgctgtta cccgcaactc atgacggcca gggcgatgaa
     4861 gtcgcccacg aggctggcat gcacgaccaa gcgccctatc gacaggtgcc ctcagaagac
     4921 gtgagcccgg caaccgatca cggtacaccg agtgtacgga tcgaggtgcg ccgcgctggt
     4981 ttcacagatc ttcttgcgct ctcccgcttc tcctcccgct accagttgaa tcagccaggc
     5041 agctcacaca ccgatagcga tccggcccgg gcgacactgc gcggatactt gccgttctcg
     5101 cgagaagacc aacctgtctt cgtggcgacg aatgaggaag agcggcgtct gcttgggttt
     5161 gtgcagttcc gtgttgctgg gccggaccag cgatggatcg cagaatcaat cggcgcaaat
     5221 gccggtgtct atgatcccga gcctgtcgtt ctcgagttgg ttcgtcatgc gatcaaggcc
     5281 gcggggttga gcggtgtgaa gcggctctat gcacgtctcg aagcggagtg cccgatcgag
     5341 caaccgatgc ggcaaattgg ctttacgcct tacggacggg aacttgtgat ggcatcgcct
     5401 tcagtgcccg ttctcccggc tgctcgcggc gtccgaatcc aggagcaggc tgatgtctgg
     5461 gccatccacc agctctacct gcagtccacg ccgcgggatg tgcagtacgc cgaggcgctc
     5521 accagccata gctgggatgt ggacgccgtc ttgcgtagcc gggggtatgg atgccggggc
     5581 tggcttatag cagatcacca cctggcggtg gcctatgcga gagcgatcac cagaagggat
     5641 gcccacatcg tcgaccttat ggtgttgcct gaccatcgag atgtcctccc ggcattgctt
     5701 tcctccgttt ttcgggaatt ggcaaccctc ccttcccgcc tggtgtacgt tgtggttcgc
     5761 cactaccaat cggagattgt cgtcgtgctg gaggatctgg ggttcgttgc ccaaatagag
     5821 caggaggttc atatccgcta tacgacggca tcggtgcggt cctctgtgat ggcggcacat
     5881 tattccgttg ccgattcaca aaaagagccc gcagtgaagc gcgtaccgac gtttttccag
     5941 ggcaccgcgg acaggtacta catgcaggaa ggagtactgg ctcctgaaga ccggcgaaat
     6001 gaggcacctc gcgcttttga aacgggtggg tagtcgtacc attggcggct gtcacctatc
     6061 taccagacgg agagtgaatt gagcagtttt cctcaagcgc tgcccgacga gcacaccaga
     6121 tcggctgccg agaccgaggt aacggacgat atccgcgagt tacttgaggt gttgccggag
     6181 gcgatttcac gacgagtgat ccagcttcag gacaaccatg agtctggcga actcattgaa
     6241 atagtgctgg acttgggacg accaccagaa gcccgtttcc agcatgacga gatcgatctt
     6301 ggagacctgg atgtctctcg cgccgatctt gggcacgttg ctgagcgcat cggacagttc
     6361 ggcgacgata atcgtgctgg aattgagcgg acactccacc ggatctcggc aatccggaac
     6421 cggtcaggtg aaatcgttgg tctgacgtgc cggatcgggc gagccgtcta tggcacgata
     6481 gcgatcatca gcgatctcat cgagtcaggg cagagtgtgc tgctgcttgg ccggccaggc
     6541 gtcggcaaga ccacgctcct gcgcgagact gcccgggtcc tggcggatga gttacggaag
     6601 cgcgtgattg ttgtggacac cagcaacgag atcgcgggtg acggagatat tccgcatccg
     6661 gcgatcggtc gcgcccgccg catgcaggtc ccgacgccca cggcacaaca tgcggtgatg
     6721 atcgaagccg tcgagaacca catgccgcag gtcgtcgtga tcgacgagat tggtacagaa
     6781 ctggaggcga tggcggcccg cacgatcgca gaacgaggcg tccagctcat tggtacggcg
     6841 cacggtaaca gcctcgaaaa cctgatgctc aaccccacgc tcaccgatct catcggaggc
     6901 atccagtcgg tcacactgtc ggatgaggaa gcccgtcggc gcggcacgca gaaatcgatc
     6961 ctggagcgca aggccccccc gacattcgga gtgatggtgg aacttgtgga tcgggacgaa
     7021 gtcgtggtgc atctcgatgt ggcagcgacc gtcgacgccc tcttgcgtgg tgctcccgtt
     7081 cggtcggagc accggcaccg aaagctggat ggcacaatcg agacgagttc ctccgaggat
     7141 gtacgtcggg atccgagctc cccaagacga ggaccatcgg tcgtcaaccc ctcttcgcgg
     7201 aatgccccac gcaccacgaa cgatcgcgga gagcggaact tcgctgacat tttcgcgagt
     7261 ggggagccgc gggacgagga gcgatcgaca gaattgctct ctaggggtca tcgacacctc
     7321 acagccatcg ataccactga agcgaccgtc cagccacgag cagcggcgcc ggctgttact
     7381 gcgttcgcca agggccgtgg gaccgacgac aagccgctga gaatctttcc gttcggcgtg
     7441 agccggaatc ggcttgacca gtcgatctct cgcctgaagg tgcccgctgt catcgttcgg
     7501 accgagcgag acgcagacat cgtgttgacg ctcaagaatg cgtatcgtca aaaggcgcag
     7561 cctgttcggg atgctgagat acgaggtatt cccgtgtatg tgctgaggtc gaacacgaac
     7621 acgcagatgg agaacgtcct gatgtcgatc ttcccgcaag cgaggatcga ggagcaggag
     7681 gatgacggcg acggcgggga cgaacccgcg cccagccgac gggagcgccg gacgaacgat
     7741 gtggtcatcg ccatgtcgga ggcggaagag gtgatctctg cagttatgca aggagcgcct
     7801 gcgacagcgc ttcgtccgca agcgccgccg atccgacgga tgcagcatga gatggccgag
     7861 cggtacaacc tggcgtcgag gagccggggc cgtgacccat tccggcatgt ggagatctat
     7921 cggcggggca tgcagtaggt gcggggccca ttcatcacac tcgaaggccc ggagggtgcg
     7981 gggaagtctg ttcaggcagc gtttctcgca tccttccttg aggagcaggg ctgggatgtc
     8041 cttagcacac gggaaccggg agggaccggc tttggggatg ccctccggga gattcttcta
     8101 ggcacggatg gtgtagcgat tctcccggag gcggaagtgc ttttgctggc ggctggccgc
     8161 gcccaacatg tccgtgagcg cattcttccc gcccttgacg ccggggttgc tgtggtctgc
     8221 gatagattcg tggattcgac cctggcctat cagggtggtg gacacgggat tccgcttgag
     8281 cagctcttgc caatccaaaa attcgcgacg ggtggtctcg agccggattt acggattctc
     8341 ctcgatgtcc cggtcgatat agggctcagg cggagacacg ccgatccctc tctggttaat
     8401 cggattgacc ttgcgtcacg ggagtttcat gagcgggtac gggctacgtt cctgcagctc
     8461 gcgaaggcga atccggaggg ctggagcatc atcgacggcc gggaagatgt ggcatcggtc
     8521 gcccgaaaga tgcacactga cgtgcagaga cgcgtacttg ccaggttcgc tgtgccaaat
     8581 ccgggagtgg tatgatcgcg ctgccgagga tgtgatgtca tgaagctcgt gatcgccgtt
     8641 gtccaaggaa aagatgcgga gccgctgctg gaagcgctga ccacccacgg gttccgcgcc
     8701 acgcagatca acagcgctgg cggattcctg cgcgagagta acgcgacgct gatgatcggc
     8761 gttgatgacg gcgaggtaga gcgcgttcgt tcgctcgtgc aggagaattg tcattcccgc
     8821 acccggttcg ttaatcccct gatgccgatc gctgacgtag ctgaatttta ttccagcagc
     8881 ccggtcgagg tgctcgttgg cggcgcaacg ctattcgtac tatctgtgaa tcgctacgaa
     8941 cgtctcgaag cttcataaat ttattcccgt ggggtctgcc aaaggagcag ttgtttggaa
     9001 atcgattcgt tcattgcccg tgcgttacag atccttctgg ccctcggtgg cgcttatctt
     9061 cttgcaacct ggttcgtatt ggcgatatgg accttccggg atatcgaggc gcgcagccgc
     9121 aacgcagtca cccaaatttt ttccacgctg ctggccgtct ttttctggat tcctggcgtg
     9181 ctgctctaca tgctgctccg gccgaaagag accctggacg aggcttatca acggtcgctg
     9241 gaggaggagt atctgctcca ggatctgcag gaacttccac tctgtccaac gtgcaatcac
     9301 ttcgtgcatg acgattggca gctctgccct cactgcaata cgcagcttcg cgaaccgtgc
     9361 ggcaactgcc ggcaccttgt agacctgcgc tgggatatct gtccctattg cggtcaggag
     9421 caggaggccc atgtcccgca tgtccacgag aagaaagcgg acggcggcaa gcatggctgg
     9481 atcgatccag cgatcatcga gaagcgcctg cgggacgagg aggcccggcg tcagcgtgaa
     9541 gcgccacgtg ccctgccagc cgccaccgcg atcgatcgcg atctcctgag tcctgctgcc
     9601 cagaacctaa cccaggaaac gacggttatc accgcgaggc cttcacagga tggtcgagcg
     9661 ccattgacac aggtaggcaa gtcacctgta gggccagaca tcaccgatct ccggggtgac
     9721 ggcagcaacg ggaccgaaga aaaggttggg gagccggtga aaattccgag aaaattccgc
     9781 atcaacgccg actaaagcgg ctttcatata tcctgcctgt ctggcagact gtgggggatg
     9841 atgtgcccgc acgcctctca cgacgtccgt gagcgagccc tggccgcagt ggatgacggt
     9901 catcgagtgg ctgacgtcgc cgcaatgtat gccattgatt catccaccct tcgccgctgg
     9961 caccggcagc gtgcccgcac gggctcctgc cgggcccgcc
//
LOCUS       DASYWM010000314         4647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_298287, whole genome shotgun sequence.
ACCESSION   DASYWM010000314 DASYWM010000000
VERSION     DASYWM010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298287"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2603)
                     /locus_tag="VGR29_12440"
     CDS             complement(<1..2603)
                     /locus_tag="VGR29_12440"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV2074437.1"
                     /translation="MSDRTLTPKSTLSSVAHPAVSAESADEIQSAIDQFSTMYPFPLD
                     DFQREAITTLLRGDSVMVAAPTGSGKTVVAEFGIYDAFKRTGRVIYTAPIKALSNQKF
                     RDLRVIYGQEVGLLTGDVTENRDARIIVMTTEILRNMLLQSPWELDDVETVIFDEIHY
                     LADPDRGTTWEESIILCPDHIQLICLSATVTNASEISGWIGRTHRPIRLIVHNKRPVP
                     LALYYFHEHELTKVIDHHGKRVAHFSNVGGEARRNRGQPRKPGGRQREVSDEPQPHEI
                     VDALKEQNLLPAIYFLFSRNDCQQFAERLAMMRPQLTTSAQAEQIEQVLASYLANIRP
                     EDQELEQVQAVLQLARKGIGFHHAGLLPILKQMVEVLFGRGLMQVVFATDTLALGVNM
                     PARTVVIGRMTKWDGRRRRVLIPNEFQQMAGRAGRRGMDVYGHVVIPYSAYIPYKEAM
                     EIATGDLHPVQSAFAIRYNTVLNLWDPPRGERVRQMLQRSLAQYQTSQRIRIIEHDVL
                     EIEAELVALTAGRAEEIARADELYEEYASLSRSIQTLEHEERKIRQEISSVRTDIETA
                     TPWTEPGRLALRKLLRSVPAGLVVHTRDHGWSIFLGRGGQGGVGLFMSQSDGQIHLLH
                     EYRLIDYLQEDARVDLPDALIEPPDMVRDPFEIVDRETLDGVMAALAALDLPDLDELA
                     RAHRELIRQQTDEKVSKLESELMEIDSQIAQLNEQKDTHPFRDTRTRKEHQKSLRQRD
                     TLEQEKNTLADILDREIDAEDVRIRGIIRGIRDILHRFGYLRQGYPTEKADMLADIFD
                     NDGLILCELIDRGILDPLKPEELAEVFSWFSFDRDFRYTNGFSLPNRLIATRRRLEDL
                     EHDILGEERDL"
     gene            2768..3850
                     /locus_tag="VGR29_12445"
     CDS             2768..3850
                     /locus_tag="VGR29_12445"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV2074438.1"
                     /translation="MRIGINAALLGAKQGYRQTGVSRYIGELVDGLNDTKAPGDKVIL
                     LGRKLGFITDAPSARILWEQTGLPASMALHRLEVLHGPMHVVPILTRIPSVMTVHDLA
                     FLRFPGQLPGNRRAFLVAATRMSAHKVDRIITVSHNTADDVIDWLKVPEEKVEAIPLA
                     PSSRVRPVSGRSLDVFRMKWNIDRPYVLAVGTLEPRKNLPLLLRAFARIKDEVDHQLV
                     LVGPEGWLTAELRQTLNELDLGDRVRLTGFVSDEELGGWYSAADLFAFPSLYEGFGLP
                     SLEAMRCGAPVLASNSSCFPEVISDAGVLIPPTDESLWAETMRELLRDPDRRGGLARR
                     GLARAGEFSWRRTAEETYRVYTEIGR"
     gene            3847..>4647
                     /gene="thiL"
                     /locus_tag="VGR29_12450"
     CDS             3847..>4647
                     /gene="thiL"
                     /locus_tag="VGR29_12450"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEV2074439.1"
                     /translation="MTALPRVRDVGEFGLIAALNAVLPHTVRVNGRVTLGIGDDAAIW
                     APTSGTTSVITTDTLIEGRHFRLDWTDWRSLGHKMLAVNLSDIASMGAMPVLATITLG
                     LTGDEHVADLEELYRGAVALAEPHDVAIAGGDVVRTTGPIVLSVTVVGEGEPLLRRDG
                     ARPGDRLVVSGTLGASAAGLRLLESPETRSGATTADLLVGAHLRPNPRIALGKVLARY
                     GATAAMDLSDGLLGDLPKLLAASGVGARLDSRLVPVIPAVRALFPIHWL"
BASE COUNT          842 a         1355 c         1475 g          975 t
ORIGIN      
        1 ttcaaatcgc gctcttcgcc gaggatatcg tgctccaggt cttcaagtcg gcgccgtgtc
       61 gcgatgagcc gattagggag tgagaacccg ttggtgtagc ggaaatcacg atcgaagctg
      121 aaccaggaga agacctcagc aagctcctcg ggtttcagtg gatccaggat cccccggtca
      181 atcagctcgc acaggatcag gccgtcgtta tcaaagatgt cggccagcat gtcggccttc
      241 tcggtcgggt aaccctgtcg caggtacccg aaccgatgga ggatatcccg gatcccccta
      301 ataatgcccc ggatgcggac atcctcagca tcgatctcgc ggtcgaggat atccgccagg
      361 gtgttctttt cctgctcgag cgtgtctcgc tgtcgcaggg atttctggtg ctccttgcgg
      421 gtcctggtgt cgcgaaaggg gtgggtgtcc ttctgctcat tgagttgggc aatctgggaa
      481 tcgatctcca tgagctctga ctccagcttg cttaccttct catcggtctg ctggcggatg
      541 agttcccggt gtgcacgggc cagctcatca agatcgggta gatcgagtgc ggctagcgcg
      601 gccatcaccc cgtcaagcgt ctcccggtcg acgatctcaa atgggtcacg caccatgtcc
      661 ggcggctcaa tgagcgcatc cggcaggtcg acccgcgcat cctcctgcag gtagtcgatc
      721 aggcggtatt cgtggaggag atggatctgg ccgtctgatt gcgacatgaa gaggccgaca
      781 cctccctgtc caccacgtcc caggaagatg gaccaaccgt ggtcacgagt atggacgacc
      841 aacccggccg gtactgagcg cagcaatttt ctcagcgcga gacgaccggg ctcggtccac
      901 ggggtggcgg tttcgatatc cgtgcggacc gaggagattt cctgccggat cttgcgctcc
      961 tcatgctcca gcgtctggat ggaacggctg agactggcgt actcctcgta gagctcgtcg
     1021 gcgcgcgcga tctcctcggc tcgtccggct gtgagcgcaa caagctccgc ctcgatctcc
     1081 agcacatcgt gctcgatgat ccggatgcgc tggctggtct ggtactgcgc gagactgcgc
     1141 tgcaacatct ggcgcacgcg ctccccgcgc ggcgggtccc agagattgag cacggtgttg
     1201 tagcggatag cgaacgccga ttgcacggga tgcaagtcgc cggtcgcgat ctccattgct
     1261 tccttgtacg ggatgtaagc tgaataggga atcacgacat gaccgtagac gtccatgccc
     1321 cgacgcccgg cacgaccggc catctgctgg aactcattcg ggataaggac acgccgccgt
     1381 ctgccgtccc atttggtcat ccgtccaatg acgaccgtgc gggctggcat gttgacaccg
     1441 agggcaagtg tatcggtcgc aaagacgacc tgcatcaggc cgcgaccaaa gagcacctca
     1501 accatctgct tgagaatggg taagaggccg gcatgatgga agccgatccc cttccgggcc
     1561 agctggagca cagcctggac ctgctcaagc tcctggtctt cgggccggat attcgccagg
     1621 tagctcgcca ggacctgctc gatctgctcg gcctgcgccg aagtagtaag ctgcggccgc
     1681 atcatcgcca gtcgctcggc gaattgttgg caatcgttcc ggctgaagag aaagtagatc
     1741 gctggaagga gattctgttc ctttagtgcg tcgacgatct catgcggctg tggctcgtcg
     1801 ctgacttcgc gttgtcgtcc ccccggtttt cgaggttgtc cccggtttcg gcgtgcttca
     1861 ccgccaacgt tggagaaatg cgcgacccgc ttgccatggt gatcgatcac cttggtcagc
     1921 tcgtgctcat ggaagtagta gagcgcaagc ggaaccggtc tcttgttatg cacgatgagg
     1981 cggatgggac ggtgggtacg cccgatccag ccggaaatct cacttgcatt cgtgacagtg
     2041 gcggagaggc agatgagctg gatatgatcc gggcagagga tgatcgattc ttcccacgtg
     2101 gtcccgcggt cgggatcggc aaggtagtga atctcatcga agatgactgt ctcaacgtcg
     2161 tcaagttccc acggactctg aaggagcata ttccgcagaa tctcggtggt catgacgatg
     2221 atccgggcgt cccggttctc cgtcacatct ccggtgagaa gaccgacctc ctggccatag
     2281 atgacgcgaa gatcgcggaa tttctggttg gaaagtgcct taatcggagc agtgtagatc
     2341 acccgcccgg tacgcttgaa cgcgtcgtag atgccgaact cggcaacgac ggttttgccg
     2401 gagccggtgg gcgccgccac catcaccgaa tcaccacgca gtaatgttgt aatcgcctcc
     2461 ctctggaaat cgtccagcgg gaacgggtac atcgtgctga attggtcgat cgcgctttgt
     2521 atctcgtcgg ctgattccgc ggacaccgcg ggatgggcca cggaggatag ggtggatttg
     2581 ggggtcaagg tgcgatcgga caagcctcaa tactccataa caggatatcg tgtggggaag
     2641 ttgactgttc gtggaaattt ggcagctctc ggacgtccct gccggacatc gcattcgttt
     2701 cctcttacag tataggactg acggtgtcac aggtacgatg caacatccga cggggaaggt
     2761 gaaaagcgtg cggatcggga tcaatgcagc ccttctggga gcgaagcaag ggtatcggca
     2821 gacaggtgtc agccggtata tcggtgagct cgttgacggg ctcaacgata cgaaggcccc
     2881 gggtgacaag gtcatcctac tcggccgcaa actggggttc atcactgatg ctccatccgc
     2941 gcgcatcctc tgggagcaga cggggttgcc cgcatcgatg gcacttcatc gtctcgaagt
     3001 cctgcacggg cccatgcacg ttgtgcccat cctgacccgt atcccgagcg tcatgacggt
     3061 gcatgacctc gcctttctcc ggttcccggg tcagcttccc ggcaatcgtc gcgccttcct
     3121 cgtggcagca acacgcatga gcgcgcacaa ggtggatcgg atcatcaccg tctcgcacaa
     3181 caccgctgat gacgtcattg actggctcaa ggtaccggag gagaaagttg aggccattcc
     3241 tctggcgcct tcatcgagag tgaggccggt ttccgggaga tcgctcgatg tcttccggat
     3301 gaagtggaat atcgaccgcc cttatgtgtt ggcggtcggc acgctggagc cccgcaagaa
     3361 cctgccgctc ctgctgcgtg ccttcgcgag gatcaaggac gaagtagatc atcagctggt
     3421 actggtcgga cccgaggggt ggctgaccgc agagctgagg cagacattaa acgagctgga
     3481 cttgggcgac cgtgtgcgat tgaccggctt tgtttccgat gaggagttgg gcggttggta
     3541 ttccgccgcc gatctctttg ccttcccttc cctgtatgag ggtttcgggc tgccgtcgct
     3601 ggaggcgatg cggtgtggag cgccggtcct ggcatccaac tcctcgtgtt tccccgaggt
     3661 tatcagcgat gctggtgtcc tcatcccgcc gaccgatgag tcgctctggg cggagaccat
     3721 gcgcgagttg ctgcgcgatc cggaccgacg gggcgggctg gcccggcgtg gactcgcccg
     3781 cgccggggag ttctcctgga ggcgaaccgc ggaggagacc taccgggtct atacggagat
     3841 cggccgttga ccgccctgcc tcgagtcagg gatgttggcg aattcggcct gattgctgca
     3901 ctgaatgccg tcctacccca tacggttcgg gtgaatgggc gtgtgactct gggtatcggc
     3961 gacgatgcag cgatctgggc tcccacgtcc ggcacgacct cggttatcac cacggatacg
     4021 ctgatcgagg ggaggcactt ccggctggat tggaccgatt ggcggagcct tggacacaaa
     4081 atgcttgccg tcaacctttc cgatatcgcc tcaatgggcg cgatgccggt gctcgcgacg
     4141 atcacccttg gtttgacggg cgacgagcat gttgctgacc tggaggagct ctaccgcggc
     4201 gccgtagccc tggcggaacc gcacgatgtc gctatcgcgg ggggcgatgt ggtgcgcaca
     4261 actggtccca tagtgctctc cgttaccgtc gtgggtgagg gggaaccgtt gctccggcgg
     4321 gatggcgcgc ggccgggtga ccgattggtc gtcagcggta cgcttggagc ttcagcagcc
     4381 gggttacggt tgcttgaatc gccggaaacc cgatcggggg cgacgaccgc cgacctgctc
     4441 gtcggtgctc acctgcgtcc aaatccgcgt attgcgcttg gcaaggtgct tgcccgttac
     4501 ggagcaaccg cggcaatgga cctgagtgac gggctgctgg gggacttgcc caagctgctg
     4561 gccgccagtg gtgtcggcgc gcgcctggac agccggctgg taccggtcat tcccgcggta
     4621 cgggcactct tcccaatcca ttggctc
//
LOCUS       DASYWM010000315         6299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_486446, whole genome shotgun sequence.
ACCESSION   DASYWM010000315 DASYWM010000000
VERSION     DASYWM010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486446"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VGR29_12455"
     CDS             complement(<1..200)
                     /locus_tag="VGR29_12455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922723.1"
                     /note="The point of entry for the majority of electrons
                     that traverse the respiratory chain eventually resulting
                     in the reduction of oxygen; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEV2074440.1"
                     /translation="MGLIDDRFDKNVFTTSLDFLFNWGRRSSLWWLQFGLACCAIEMI
                     SASMARFDLSERFGMLFRSSPR"
     gene            complement(191..556)
                     /gene="ndhC"
                     /locus_tag="VGR29_12460"
     CDS             complement(191..556)
                     /gene="ndhC"
                     /locus_tag="VGR29_12460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEV2074441.1"
                     /translation="MIILGENWIAIVFMIVATFAMGMLLLTLARIVAPTKPSEAKSRP
                     YESGIPDVKPVRPQFTARFYVIAMLFVIFDLEAAFMFPWAVNFDLLGLNGFMHMLVFI
                     ALLFVSYAYAWKKGALEWV"
     gene            complement(1150..2592)
                     /locus_tag="VGR29_12465"
     CDS             complement(1150..2592)
                     /locus_tag="VGR29_12465"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEV2074442.1"
                     /translation="MGEHQLRRRPGNRVVRRRLHATNVIDLPKETETRDVPTATAHAK
                     QFVVGVSLLIVVGALLLMLPIASESGESTHPTDAFFTAMSAASVTGLVTVDTATHWTF
                     FGELVILVLIQLGGFGFMVGASVVLVALGRGSSLRDTLMMQDGSPTMSLREVTSLSRR
                     IIRFMLITEAIGAILLTAHFLQHESPLNAIWHGIFHSVSAFCNAGFDLQGGFNSVIGL
                     SGSPLVILTIGGLIQAGALSFMVLNDVWVKRKWRDLYLDSKLVLITNFLLIIAGMMFF
                     LIVEWNTALSRMDAELRPMNALFQSVAARTAGFASVNFNDAQPATLFLWVGLMLIGGA
                     AGSTAGGIKLATIAILFLAIVATLRGQEEPQTFGRRIAPNLIFRSLAIVALFMAMHFM
                     LALTLAITEDVFNDAAFGFAPLMFEAMSGLATVGLSTGITPELSIPGKLVLFVAMFIG
                     RLGPITAAYALQRRQQPARFRFPEAHVRIG"
     gene            complement(2595..3302)
                     /locus_tag="VGR29_12470"
     CDS             complement(2595..3302)
                     /locus_tag="VGR29_12470"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV2074443.1"
                     /translation="MPDSRRNGSLERVTVIGLGRFGTSVAKTLHELGYEVTAIDLREK
                     NIQSAADYVTLAAQGDGTDQELLEQLNVDKSDVAVVAQGESLEASVLSTLLLKKLQVP
                     WVISKAKTVLHGELLQKVGANQVVFPENDAGIRLAHSLGVRHISDYITLSPSAGIAKI
                     EAPANLVGHTIEECTRGQQAKMNVLLIKRGNQLITVPHYQEVIMAHDQLVIVGADADI
                     ETFIQRDLASPGTKLAR"
     gene            complement(3495..4484)
                     /locus_tag="VGR29_12475"
     CDS             complement(3495..4484)
                     /locus_tag="VGR29_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2074444.1"
                     /translation="MTMHYGTIDGVDKPVSRLVQGTIPLSSGREEESFRLLDEIFELG
                     CRTFDTAHNYGRGDCETVFGAWMRDRGVREEIVVLGKGAHPYDGRERVTPEDITADLH
                     DSLARQQHEYFDMYLLHRDNPSLPVGPIVEVLNQHKRDGKISIFGGSNWSTSRIAEAN
                     TYARENGLEPFAVSSPNFSLAVQIQPPWEGCISISGDVGKADREWYRENNMPIVPWSS
                     LAGGFFSGRFTRDNVDEMAANGDYFEKLCVASYCSEENFQRLDRVREMAERDDMTIPQ
                     VAMAYVMSQPQEIFALVGCRNGNEFAENAAMLDRRLTQEELDWLDLTSDNRPW"
     gene            complement(4523..5551)
                     /locus_tag="VGR29_12480"
     CDS             complement(4523..5551)
                     /locus_tag="VGR29_12480"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2074445.1"
                     /translation="MVTEPGNTPFIPPGQPVRWGIIGTGKIAGIFANDIPHADNATLQ
                     AVGSRSQASANAFGDTYNIPRRYDRYEDLANDPEVDVVYVATPHPAHHDVTIMCLEAG
                     KHVLCEKSFAMNAAEALEMIDAAKKNNLFLMEAMWTRFRPLMYKVREIVNSGEIGEVQ
                     FLTANIGWTATFDPEFRLYAPELGGGGLLDAGVYPVSFASMVLGKPDQVCGVATLGQT
                     NVDENAMIAMHYPSGAVASVGFTIRSSPVSLGMICGTRGTILIDHDWHRPTTFSLRKP
                     GEEPQHFDYTLKAGVGYQFEMNEVERCLREGRTESDVMPLSETLTIMETMESVLREWG
                     VRYPSDRN"
     gene            5618..6238
                     /locus_tag="VGR29_12485"
     CDS             5618..6238
                     /locus_tag="VGR29_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074446.1"
                     /translation="MIDANRKQQNAIVVSLCGPSNAGKSQLAKATVAALGSEVASRVP
                     VDYFFIPRPESVSLDAFFSQPLGWDWDLLRERLALPVGTRTSTPDADFDAFQRRAPDG
                     GPGFTVRPIMLCDAMAPFPQSDLIALIDVSDTQRLRRVAERDLPWGTSVGNRWMHLEE
                     TWGNVSKELGPDLVLDGMEPLAVNAGNLCQTISALFVIDRTTTIEE"
BASE COUNT         1295 a         1826 c         1907 g         1271 t
ORIGIN      
        1 tggcgggggg atgagcggaa gagcataccg aagcgctcgg agaggtcaaa gcgcgccatg
       61 gaagcgctga tcatctcgat cgcgcaacag gcgagaccga actgcaacca ccacagggaa
      121 gaacgccggc cccagttaaa aagaaagtcc agagatgtcg tgaagacgtt cttgtcgaag
      181 cgatcgtcga tcaaacccat tcgagggcac ctttcttcca ggcatatgca tagctgacga
      241 acagcagtgc gataaagacc aacatatgca tgaaaccgtt caaaccgagt agatcaaaat
      301 tgactgccca tggaaacatg aatgcagctt ccagatcgaa gatgacaaag agcatcgcga
      361 tcacataaaa ccgggcggta aactgtgggc ggactggctt gacatcggga ataccgctct
      421 cgtagggtcg tgatttggcc tcactcggtt ttgtcggggc cacgatccgg gcgagcgtca
      481 ggaggagcat gcccatcgcg aatgttgcga cgatcatgaa gacaatcgca atccagttct
      541 cgccaagaat tatcacgaat cccgctccac gctgaggttc aatcggcagg tccaccgtca
      601 ggaaaagtac ggacgttgaa gccgcgaagc gcttcctgcc ccagcctgtt cctgctcccg
      661 gatggggtgt gggtcggtgg caggtgacac gttaaggggc gtgcacacgc aggccggaaa
      721 cgcccacatt tcgtgtgaat ggtagcacaa tgagcccggc cctcttcccg tgccaagccc
      781 cccaatgacg ggagagcagg ggccgccgat ggagtatttg tgaatcgttg ccggacatcg
      841 cagtgggaat caccgggtcg tccagaggct ggacaacagt gacgcagact aggtgctcga
      901 aggggtatga acctattttg gccaggggca tgcgtatgga cccggaagtg tatacattcg
      961 gaccatttgt catcgccacg ggcactctag tattaggtca acaccaacgt gatcgtgtcg
     1021 ccagccacgg ctcgatcgtc ccccttccct ccaacgtggt gtttggtagc cgcacgggcc
     1081 atcccccgtg cggttgcttt gtttaacagg gactctccta ttgctgcaac cacgatggtg
     1141 atctcacgcc tagccgatgc ggacatgcgc ctccggaaac cggaagcggg ccggttgttg
     1201 tctgcgctgc aaagcatagg cggccgtgat tggtcccagt cggccgatga acatggccac
     1261 aaaaagcacc agcttgccgg ggatcgagag ctcaggggtg atgcccgttg agaggccaac
     1321 tgtggcaagg ccgctcattg cctcgaacat cagtggtgcg aagccgaacg cggcgtcgtt
     1381 gaagacatcc tcggtgatgg cgagtgtcag ggcgagcatg aagtgcatcg ccatgaaaag
     1441 agccacgatc gccagcgatc ggaaaatgag gtttggtgcg atgcgtcgtc cgaacgtctg
     1501 gggctcctcc tggccccgca gggtagcgac gatcgccagg aagagaatgg cgatcgttgc
     1561 cagcttgatg ccgcccgcgg ttgatccagc tgcgcctcca atgagcatga gtccgaccca
     1621 gaggaagagc gtggctggct gcgcatcgtt gaagttgaca ctggcgaatc ccgcggtgcg
     1681 ggcggcaact gactgaaaaa gggcattcat cggccgcagc tccgcatcca tgcggcttag
     1741 ggcagtgttc cactccacga tcaggaagaa catcatcccg gcgatgatca gcagaaaatt
     1801 ggtaatcagg accagcttgg aatcgagata gagatcgcgc catttgcgct tgacccacac
     1861 atcgttcaag accatgaacg agagggctcc cgcctggatg agcccgccga tcgtgaggat
     1921 caccagggga gatcccgaga ggccgatcac agagttgaac ccaccttgca ggtcgaatcc
     1981 tgcgttgcag aaggccgaga cggagtggaa gatcccgtgc cagatggcat tgagcggcga
     2041 ctcatgttgc agaaagtgag ccgtgagcag gattgctccg atcgcttcgg taatcagcat
     2101 gaagcgtatt atgcgccggg aaagcgacgt gacctcacgc agggacatcg tgggggagcc
     2161 gtcctgcatc atcagcgtgt cgcggaggct ggacccgcgt ccaagggcaa cgaggaccac
     2221 cgatgccccc accataaatc cgaacccacc gagctggatg aggacgagaa tcaccaattc
     2281 cccgaaaaac gtccagtgtg tggcggtatc taccgtcaca aggcccgtga cagatgccgc
     2341 ggacatcgcc gtgaagaagg catcggtcgg atgcgttgac tcaccactct cgctggcgat
     2401 cggcagcatg agcagcaagg cgccaacgac gatcagcagg ctgacaccaa caacgaattg
     2461 cttggcatgg gcggttgccg tagggacatc gcgagtctcg gtctctttcg gcagatcgat
     2521 cacgttcgtc gcatggagac gccggcgtac cactcgattg ccgggacgcc tgcgcagctg
     2581 atgctcgccc acggctacct cgccagtttg gttcccggtg aggccaggtc ccgttgaata
     2641 aacgtctcaa tgtcggcgtc ggcaccgacg atcaccagct ggtcgtgcgc catgatcact
     2701 tcctggtagt gaggaacggt gatgagctgg ttgccccgct tgataaggag gacgttcatc
     2761 ttggcctgct ggcctctggt gcactcctcg attgtgtgtc cgacgagatt ggcaggtgcc
     2821 tcaatcttgg cgataccggc cgagggcgag agcgtgatgt agtcgctgat gtggcgcacg
     2881 ccgagggagt gggcaagccg gatgccggca tcgttttccg ggaaaacaac ctgattcgcg
     2941 ccaaccttct ggagcagctc accatgaagg accgtctttg ctttcgagat cacccacggc
     3001 acctgcagct tcttgaggag cagcgtgctc agcacgctgg cttcaagact ctctccctga
     3061 gcgaccactg cgacatcaga cttgtcgaca ttgagctgct cgaggagttc ctggtcggtg
     3121 ccgtctccct gagccgcaag cgtcacatag tccgctgccg actggatgtt cttctcccgg
     3181 aggtcgatcg cggtaacctc gtaaccaagt tcgtgaagcg ttttggccac gctggttccg
     3241 aagcgcccaa gaccgatgac agtaacgcgt tcaagtgagc catttcgtcg ggaatcaggc
     3301 accgtctgca ctatcctctg tgtgagtgac gctctttacc gatcacctga cggctcctgc
     3361 cgtcccggta tcgtgacgat tgtacgcagg ttcccggtcc atgtccctcg tcgaccctgc
     3421 gctgtagttg gcggtgtagc agggggagat cgtggtgagg gcctccggtg atctgattga
     3481 taggatggca gaagttacca gggacggttg tcgctggtga ggtcgagcca gtccagctct
     3541 tcctgcgtca gcctgcgatc cagcatcgct gcgttctcgg cgaattcgtt gccgttccgg
     3601 caaccgacca gggcgaagat ctcctgaggc tggctcatga cgtaggccat ggctacttga
     3661 ggaatcgtca tgtcgtcccg ttcggccatc tctctgacgc ggtcgaggcg ctggaaattc
     3721 tcctcggaac agtacgaagc aacgcagagc ttctcgaagt agtccccgtt cgcggccatt
     3781 tcatccacgt tgtcacgggt gaaacgcccg gagaaaaagc cgccagcaag gcttgaccat
     3841 ggcactatgg gcatgttgtt ctcccgatac cattcccggt cggccttgcc gacatcaccg
     3901 ctgatgctga tgcacccttc ccatggaggc tggatctgga cagcgaggct gaagttgggg
     3961 ctgctcaccg cgaacggctc taacccgttc tccctggcat acgtgtttgc ctcggcaatc
     4021 cggcttgtcg accagttcga gccaccgaaa atgctaatct tgccgtcccg cttgtgctgg
     4081 ttcagcacct cgacgatcgg tcccacgggc agggacgggt tgtccctatg cagcaaatac
     4141 atatcgaagt actcgtgctg ttggcgtgcc agggagtcgt gcaggtcggc ggtgatgtct
     4201 tccggcgtga cgcgctcgcg gccatcgtag gggtgcgccc ccttgccaag gacgacgatc
     4261 tcctcacgca ccccgcgatc ccgcatccag gcgccgaaca ccgtctcgca atcgcctcta
     4321 ccgtagttgt gggcggtatc gaaggtgcgg cagccgagct caaagatctc gtccagaagc
     4381 cggaagctct cctcctcccg accggaggag agtgggatcg ttccctgcac gagccgggat
     4441 accggcttgt caacgccatc gatcgtgcca tagtgcatgg tcataactct cgcgaagcct
     4501 tcctgtgttc ctgcaaccta ggttagttgc gatcgctggg gtatcggact ccccactcac
     4561 gcaacacaga ctccatcgtt tccatgatcg tgagcgtttc cgaaagaggc atgacgtcac
     4621 tctccgtgcg cccctcgcgc aggcagcgct cgacctcgtt catctcaaac tggtagccga
     4681 cccctgcctt cagcgtgtag tcgaagtgct gcggctcctc gcccggtttt cggagcgaga
     4741 aggttgtcgg tcggtgccag tcgtggtcga tgaggatcgt tcccctggtg ccgcagatca
     4801 tgccgaggct taccgggctg gagcgaatgg tgaagccgac agaggccacc gcgccggagg
     4861 gatagtgcat ggcgatcatt gcgttctcat cgacgttcgt ctggccaagc gtagcaacgc
     4921 cgcagacctg gtccggcttg ccgagcacca ttgaggcaaa ggagacgggg taaacaccgg
     4981 catcgagaag accgcctccc ccaagctcgg gcgcatagag ccggaactcc ggatcgaagg
     5041 tcgccgtcca gccaatattt gcggtcagga actggacctc accgatctca ccgctgttga
     5101 cgatctcgcg caccttgtac atcaggggcc ggaagcgggt ccacattgcc tccatcagga
     5161 agagattgtt cttcttcgcg gcatcgatca tctccagtgc ctcggcggca ttcatggcga
     5221 atgacttctc acagaggaca tgtttgccgg cttcaaggca catgatggtc acgtcgtgat
     5281 gggccgggtg cggtgtcgca acgtagacaa cgtccacctc cggatcattg gcgaggtctt
     5341 catacctgtc gtagcgcctc gggatgttgt acgtgtcacc gaaagcgttc gcgctcgcct
     5401 ggctgcggga gccaaccgcc tgcagcgtag cgttgtccgc atgcggaata tcgttggcga
     5461 agatacctgc aatttttccg gtaccgatga tgccccatcg gaccggctgt ccaggcggga
     5521 taaacggggt gttgcccggc tccgttacca tcagctatac gtcctttcat cgtgaggtcc
     5581 taggcaggaa tcctgtgcat acgtgaatgc tgccagaatg atagatgcaa accggaaaca
     5641 acagaatgcc attgttgtct cgctctgcgg gccatcgaat gccggcaagt cgcagctcgc
     5701 gaaggccacg gttgcggcgc tgggttcaga ggtcgcctcg cgggtaccgg ttgattactt
     5761 ctttatccct cgcccggaat cggtctcact cgacgcattc tttagccagc ccttggggtg
     5821 ggactgggat ctcctacggg agcggcttgc gttgccggtt ggcaccagga catccacacc
     5881 ggacgctgac ttcgatgcct ttcagcgccg tgcacctgac ggagggcctg gatttaccgt
     5941 ccggcctatc atgctgtgcg atgccatggc accattcccg cagtccgatc tgatcgcgct
     6001 catcgatgtt tcggatactc agcggctgcg gcgggtggcc gagcgggacc ttccctgggg
     6061 tacatcggtc ggaaatcgat ggatgcatct ggaagagaca tggggcaacg tatcgaagga
     6121 gctgggtccg gatctggtcc tcgacggcat ggagccgcta gcggtcaatg cggggaactt
     6181 gtgccaaacg atttcggcac tcttcgtgat cgatagaacc acgactatag aggagtaacc
     6241 atggcatttg aaccacagga tattccggcg ggaacgccgg tcgtcggtct caatgggga
//
LOCUS       DASYWM010000316         5581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_392358, whole genome shotgun sequence.
ACCESSION   DASYWM010000316 DASYWM010000000
VERSION     DASYWM010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392358"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1550)
                     /locus_tag="VGR29_12490"
     CDS             complement(165..1550)
                     /locus_tag="VGR29_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074447.1"
                     /translation="MKRFTGTGRRIGGRSGEKVLKQPRILVRSLLLLSIIYSLLYSGT
                     GVSAVAAQAGATSFTSPMTGSTIYITGSWTIDTASVASQDGIESITLTGPYDFMQVWF
                     MPAGLDLVAARDIILDSFATEFGTFVEIDRGAYGDVSYSLDMTSSEGVEFGVFSLFLG
                     QRASGFVEYYVYFGPVIYFQQGFAAAQQSITVNGNPVFSGVDGAGLQNLLSANAGAAG
                     GAVQPPAGEVVPGNQPPAAPTQVPAQVPAQPTAAAPPAAPTEAPAPPPSDPAAASGEG
                     AAYLTTLQSELGYIQTTITDATAQFGGLEGDNSAAAIEAISRIAAEWSAYPDRAAGIV
                     APAGYEGIHASYQALAGQVGQLGTSWSGFVDSIQTGDVTTIQSTLQTFLDQFVGIQGQ
                     VDALNAQIAESQVGSVAEAQPTAAPATAATEVAPAQDSGEGAAYISAIQAELTYLEGT
                     LSNFATKLRHA"
     gene            1898..3085
                     /locus_tag="VGR29_12495"
     CDS             1898..3085
                     /locus_tag="VGR29_12495"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV2074448.1"
                     /translation="MRTPHDFFATRVRQAAPSPTLAVSDRARQLKEEGVDVIDLGGGD
                     PDFITPEHIRQAAINAMNAGETHYVASAGTPALRKAIAQKLLDDNGITVNPTKGILVT
                     PGGKAALFQAIMAFVEPGVDVMILEPAWVSYTPMVELAGGRPVHVGLDPDENFAITRE
                     LLDEHVTPESRILLLNSPNNPTGRVATDEELSIVADFAREHDLLVFTDEMYEKIIYDE
                     NTHTSIATLPDMAARTLTFNGLSKAYAMTGWRLGYVAGPDAYIDKISTIHSHSVTCAT
                     SFAQAGGVAAYTGPQEIIHEMVSAWDRRRNLITEGVNRIKGLRVQTIEGAFYAFVDGR
                     ETGMDAVTLSRTLLDDAAVAVVPGTAFGDAGEGHFRLSFATSDAVLETSLERIGKLLG
                     TRD"
     gene            3133..3582
                     /locus_tag="VGR29_12500"
     CDS             3133..3582
                     /locus_tag="VGR29_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV2074449.1"
                     /translation="MSIDARLSELGLELPEPITPAGNYVRYQTTGNLLFISGTGPSSE
                     HKKGKVGAEVSLEEAYAAARSVGLQILATANAALGSLDRITRTVKVLGMVNSDPGFGE
                     HPKVINGCSDLLVEVLGDAGKHTRSAIGFVSLPNQISVEIEATFEFE"
     gene            3729..4202
                     /gene="greA"
                     /locus_tag="VGR29_12505"
     CDS             3729..4202
                     /gene="greA"
                     /locus_tag="VGR29_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV2074450.1"
                     /translation="MVDRKKIPFTREGFERVSKELKELVEVKRPEIVAAVAEARSHGD
                     LRENAAYDVARQDQAMIEKRISDLENKLRNAEIIEDSDRGVEGVVALGRTVTVDFDGD
                     EEVYKIVGPAEAMPIKGLISDESPMGSQLKGRKVGDRVTVKTPGGQAEILIKAVE"
     gene            4282..5109
                     /locus_tag="VGR29_12510"
     CDS             4282..5109
                     /locus_tag="VGR29_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEV2074451.1"
                     /translation="MPSEQDEAYRRIWQHLRQVSSVADGRHDSEEWRGKRGDFVMCCV
                     RIPPDALSSSLDQVHETLGTFPYVRVHPKSFLHVTVQELGFLTDEPLRRGDITREWLD
                     EFIGQAENPVGEYSPFTISLGGINSFVDAVFLDVHDGGWLSRIHGRLIDFVSVPPSNR
                     YAYLPVVTIGHYTESAPIGNLVAALTPWRDRNFGTFRAESIDIVKLRTSVPYPDMELI
                     HRFELGHQQRLIEMVQGTGSSGPSQPVISADPPVGIEAIEPITSAGSSNFKRSTRRR"
     gene            complement(5153..5374)
                     /locus_tag="VGR29_12515"
     CDS             complement(5153..5374)
                     /locus_tag="VGR29_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV2074452.1"
                     /translation="MVIPANTDVTLRLVNEGVASHNLTIDGAGIVSPVIPGGEETTIV
                     VNLPSGTYSIGCDVPGHRMAGMTGVLHAE"
     gene            complement(5379..>5581)
                     /locus_tag="VGR29_12520"
     CDS             complement(5379..>5581)
                     /locus_tag="VGR29_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074453.1"
                     /translation="TQEVVVGGLMLPNGIAFEEDGSLLIVTGATSPGEPMGQLLRCEG
                     IAVAADVTEEVVVALLDPHFRS"
BASE COUNT         1169 a         1703 c         1606 g         1103 t
ORIGIN      
        1 agctgttgct cagttgctcg atctgtcctg cgattgccct gtaggaggca tcaatatcct
       61 cataccctgg cggggcaaca actgctgccg cgcgctccgg ataagcctcc cattcatccg
      121 acacgcggtt aatctccgcg atcgcggctt cggacgtttc tccttcaagc atgccgaagt
      181 ttggtggcaa agttggagag cgtgccctcc agataggtta gctctgcctg aatagcactg
      241 atgtacgccg ccccttcacc ggagtcttga gctggcgcta cctctgtcgc tgctgttgct
      301 ggcgcggcgg tcggctgtgc ttccgcgaca ctcccgacct ggctctcagc tatctgcgcg
      361 ttcagcgcgt cgacttgccc ttgaatacct acgaattggt cgaggaatgt ctgtagcgtg
      421 gattggatcg tcgtcacatc accggtctga atgctgtcga caaatccgga ccagctggtg
      481 ccgagttgac ccacctgtcc ggcgagtgct tgataggaag cgtggatgcc ctcatacccg
      541 gccggagcta cgatgcctgc ggcacgatcg ggatacgcgc tccactccgc cgcaatacgg
      601 ctgatcgcct caatggctgc agcagaatta tcgccttcaa ggccgccgaa ttgggcagta
      661 gcgtctgtga tcgttgtctg gatatacccc aactcggact gaagcgtggt gaggtatgcg
      721 gcgccttcac cggatgcggc agcaggatca ctgggcgggg gagcaggggc ttcggttggc
      781 gccgctggcg gcgcagccgc cgtcggttgg gctggaacct gggcaggcac ctgtgtcgga
      841 gccgctggag gctggttacc cggaactacc tctcccgcag ggggctgaac cgccccaccg
      901 gccgcaccgg cgttcgccga caagagattc tggagcccgg cgccatcgac accgctgaag
      961 acaggattgc cgttcaccgt aatgctctgc tgcgctgccg cgaacccttg ctggaaataa
     1021 atgacgggtc caaagtacac gtagtactcc acaaacccac tggcacgttg accaaggaag
     1081 agtgagaaga ctccgaactc gacgccttcc gagctcgtca tatccagcga gtaacttaca
     1141 tctccgtatg caccgcgatc gatctcaacg aatgtgccga attcggtggc gaacgagtcc
     1201 aagatgatgt cccgcgcagc gaccagatcg agacccgcgg gcatgaacca gacttgcata
     1261 aagtcgtatg ggcccgtcag cgtaatgctc tcgatcccat cctgtgacgc gacagaggcc
     1321 gtgtcgatcg tccacgaacc cgtgatgtag atggtggagc cggtcatggg cgaggtgaag
     1381 ctcgttgcac cggcctgcgc ggcaacagca ctcacccccg tgcccgaata cagcaatgag
     1441 tagataatcg acagaagaag caacgatctg acaagaatgc gtggctgttt caacaccttt
     1501 tctccacttc gtccgccgat gcggcggcca gtccctgtaa accgcttcat ttgttcgtca
     1561 ctccctagcg ccctttgctt aaggatctcc gaaacagtgc agcgaagcgt catgctgtcg
     1621 gtgtgattcc tcacgtcgtg gcttctgacc catgaacgcg caccttagga aaaataacgc
     1681 cggacgggaa gtggtagttc ccatatcgcc tcgccggaat caaccgatgg ccgtttccat
     1741 tcgtgacgcc atcagggcca gcgccgcgcc gttcgtatcg cagtgggagg agttattcca
     1801 gtgttcgccg tttgcgggta aactgccagg gacacagaac taccgaacac ttcttgcgcc
     1861 tatccgcagt cgaacgagga cgcctatcgt gagcaccatg aggacaccac acgacttctt
     1921 cgcaacgcga gtacgccagg cagcgccgtc cccgaccctg gccgtctccg accgcgcgcg
     1981 ccagctcaag gaggaggggg tggacgtcat cgaccttggc ggtggcgatc cagacttcat
     2041 cactcccgag cacatccgtc aggcagcaat caacgccatg aacgcgggtg agactcatta
     2101 cgtcgccagc gccggcacac ccgccctccg caaggcgatc gcgcaaaagc tcctggacga
     2161 caatggcatc accgtcaatc cgacgaaagg tattctggtt acccccggcg gcaaggcagc
     2221 gctcttccag gccatcatgg ccttcgtcga gccgggcgtc gatgtgatga ttctcgagcc
     2281 cgcctgggtc tcctatacgc cgatggtcga actcgccggc ggccggccgg ttcacgtggg
     2341 cctcgacccg gacgagaact tcgccatcac ccgagagctt ctcgacgagc acgtcacgcc
     2401 agaatcacgc atcctgctgc tgaactcgcc aaacaatcca accggccgtg tggcaacaga
     2461 cgaggaactt tcaatcgtcg ccgacttcgc cagggaacat gatcttctcg tctttaccga
     2521 cgagatgtac gagaagatca tctatgacga gaacacgcac accagcatcg ccaccctacc
     2581 cgatatggca gcccgcacat tgacgttcaa cgggctttcc aaggcctacg caatgaccgg
     2641 ctggaggctc gggtacgtag ccggacccga tgcctacatc gacaagatct cgactatcca
     2701 tagccattct gtaacgtgcg cgacgtcctt cgcgcaggct ggtggtgttg ccgcttacac
     2761 cggtccccag gagatcatcc atgagatggt ctccgcctgg gatcgccgcc gaaatttgat
     2821 cacggagggc gtgaacagga tcaagggtct cagggtgcaa accatcgagg gcgcgttcta
     2881 cgcatttgtc gacggccggg aaaccggcat ggacgccgtc acgctgtcca ggacgcttct
     2941 cgacgacgcg gccgtcgcag tcgttcctgg cacggcattc ggtgacgcag gcgagggcca
     3001 ctttcgcctc tcgtttgcga ccagcgatgc tgtgttagag acctcccttg agcgtatcgg
     3061 caagctactc ggaacgcgcg attaagggca taacgctcga taacaacgtc tgtaaccgta
     3121 caaggagttt ccatgagcat tgacgcacga ctctcggagc tgggtcttga gctccccgaa
     3181 cccatcacgc cggcagggaa ctatgtccgg taccaaacca cggggaacct gctctttata
     3241 agtggcaccg gcccatcttc cgagcacaag aagggcaagg tgggcgcaga agtctctctt
     3301 gaggaggcct atgccgccgc acggagtgtt ggactccaga ttcttgccac tgccaatgcg
     3361 gcgcttggat cgctcgaccg gatcacgcgg actgtgaagg tccttggcat ggttaacagc
     3421 gatccaggct tcggcgaaca ccccaaggtt atcaacggct gctcggatct ccttgtcgag
     3481 gttctcggcg atgctggaaa gcacacccgg tcagcgatcg gtttcgtctc gcttcccaat
     3541 cagatcagtg tggagatcga agcgaccttc gaattcgagt agtacctgga gaagaacaca
     3601 atacaaacca gaaggcggat gcgcggtgct tgcaacagca tcagggaatg catcttgcct
     3661 tctctattca tcacgtgcaa tgacgccttc gcgcacatac cgatgtgctt taacgtttcg
     3721 aggatccaat ggtagacagg aagaagatac cgtttacgcg ggaaggattc gaacgcgtca
     3781 gcaaagagct gaaggaactg gtcgaggtca agcgcccgga gattgtggct gcggtggcag
     3841 aagctcgctc gcacggcgat ctccgggaga acgccgcata cgatgttgcc aggcaagacc
     3901 aggcgatgat cgagaagcgg atctccgacc ttgagaacaa gctccgaaac gcggaaatca
     3961 ttgaggattc agacaggggc gtcgagggtg ttgtggcctt gggccgcacc gtcacagtcg
     4021 acttcgacgg cgacgaagag gtgtacaaga ttgtcgggcc cgccgaggcc atgccgataa
     4081 agggactgat ttccgatgaa tcgcctatgg gcagccagct caagggcagg aaggtcggcg
     4141 accgcgtgac cgtcaaaaca ccgggcggcc aggccgagat cctgatcaag gcagttgaat
     4201 aatctctcgc atcgagaact ctcgcaaaaa gccatccgtt ggctgctcaa atggcgccat
     4261 cctttctggt ggctccccga tgtgccttca gagcaggatg aagcatatcg ccgcatctgg
     4321 caacatctcc ggcaggtttc cagcgtggcc gatggccgcc atgactcaga ggagtggcgg
     4381 ggcaaacggg gcgatttcgt catgtgctgc gtccggattc ctccggacgc actctcctcc
     4441 tcactggacc aggtccacga gacgcttggg acattcccat acgttcgtgt ccacccaaag
     4501 agcttcctcc atgtcacggt gcaggaactg ggtttcctca cggatgagcc actgcggaga
     4561 ggagacatta cgcgagagtg gctggatgag ttcatcggcc aggccgaaaa cccggtcggg
     4621 gagtactctc ccttcacgat ctcgttgggc gggattaact cattcgtcga tgcagtgttc
     4681 ctcgacgtgc atgatggtgg ctggctctcc cggattcatg gacgactgat tgacttcgta
     4741 tccgttccgc ctagtaaccg gtatgcctat cttcccgttg tcactatcgg ccactatacc
     4801 gagtcagcac ccataggaaa cctcgtagca gcgctcaccc catggcggga ccgaaacttc
     4861 ggcacattcc gcgcggagtc catcgacatt gttaagctgc gcaccagtgt gccttacccc
     4921 gacatggagc tcatccaccg gttcgaactc ggtcaccagc agcggttgat agagatggtg
     4981 cagggcaccg gttcctccgg accatcgcaa cccgtcattt ccgcagatcc accggtcggc
     5041 atagaagcaa tcgagccaat aacaagtgct ggctccagta atttcaaacg aagcacccgc
     5101 cggagatgat ccggcgggtg cttcttggag acttggctgg ggcgtctcgt tgctactcgg
     5161 cgtgcagcac gccagtcatt cccgccatgc ggtgacccgg gacatcacac ccaatactgt
     5221 aggtgcctga aggcaggttg accacgatcg tggtctcctc cccgcccggg atcacgggtg
     5281 atacgatgcc cgctccgtcg atcgtcaggt tgtgggacgc cacgccttca ttgacgagtc
     5341 gcagtgtgac atcggtgttt gccggaatga cgatcgtatt aggatcgaaa gtgtgggtcg
     5401 agcaaagcga ccaccacctc ctctgtcaca tcggccgcca cagcgatgcc ctcgcagcgc
     5461 agcaactggc ccatcggctc gccagggctg gtcgcacctg tgacaatcag caggctgccg
     5521 tcttcctcga acgcgatacc gttcggcagc atcaggccgc caacgaccac ttcctgcgtg
     5581 c
//
LOCUS       DASYWM010000317         2937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_505137, whole genome shotgun sequence.
ACCESSION   DASYWM010000317 DASYWM010000000
VERSION     DASYWM010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505137"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VGR29_12525"
     CDS             complement(<1..120)
                     /locus_tag="VGR29_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV2074454.1"
                     /translation="MAKQKFDRSKPHVNVGTIGHVDHGKTTLTAAISKTLAMQG"
     gene            complement(377..452)
                     /locus_tag="VGR29_12530"
     tRNA            complement(377..452)
                     /locus_tag="VGR29_12530"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(417..419),aa:Thr,seq:ggt)
     gene            complement(488..568)
                     /locus_tag="VGR29_12535"
     tRNA            complement(488..568)
                     /locus_tag="VGR29_12535"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(532..534),aa:Tyr,seq:gta)
     gene            complement(610..682)
                     /locus_tag="VGR29_12540"
     tRNA            complement(610..682)
                     /locus_tag="VGR29_12540"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(647..649),aa:Thr,seq:tgt)
     gene            complement(825..1838)
                     /locus_tag="VGR29_12545"
     CDS             complement(825..1838)
                     /locus_tag="VGR29_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074455.1"
                     /translation="MVVANTTAPASAGPLGEQSERDLTNMRQSRSLWSNALRRLRRNR
                     MAMAGILFLGFLALVAVFAPVIVPHNPVQTDVANAGTYRKAAWLTDPNPLRTGSWDYP
                     LGTDSIGRDVFSRLVYGTRVSLVVGFIPMLVILFIGVPLGLIAGYCGGRVDNLLMRMT
                     DVVYAFPALLLAIIMQISFGEYAIGKLLNGLVLLFISLSIVNWTGVARLVRGETLSLK
                     EKEFVEASRTSGSGGLRIIAKHILPNALGPIIVAAAFIVPGAIIAEAVLSYLGVGVRP
                     STNLDSPFPTSWGQMILDGSKSYTAQSWMLIAPSIAVALITLSFTFIGDGLRDALDPR
                     GAD"
     gene            complement(1851..2867)
                     /locus_tag="VGR29_12550"
     CDS             complement(1851..2867)
                     /locus_tag="VGR29_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074456.1"
                     /translation="MLQFVIRRLLWIVPVVVVVAAVTFFLMYQAPGGPFDLEKPMPEA
                     AQRTLNARFGLDKPLWTNPVEFEDRWDQGVRNPFSLARGFLDSQFFNYMGRILTLDFG
                     PTYESRGSETVQEVIVRQFPVSLRIGLVGVIFALIIGLPLGVISALRQNTWVDYISLG
                     VSTLGISVPTFVSGLLVLIFLSQTFGVSPIKRPEAWQGLFSTAYLLPGIILGLGTTAF
                     IARLTRSSVLEVKRQDFVRTARAKGVGESPVVLRHILRNALLPVVTIIGPAAADLITG
                     SIIIETIFNAPGLGKTFVSAIGKRDYSLIMGVTIFYAVLIALANVLVDLAYGLLDPRI
                     RTRR"
BASE COUNT          638 a          897 c          873 g          529 t
ORIGIN      
        1 cccctgcatc gccagcgtct tggagatcgc cgccgtcaac gtcgtcttgc catgatccac
       61 atgcccgatc gtccccacgt tcacatgcgg cttcgatcgg tcgaacttct gcttggccat
      121 tccaggtcct ccttgccccg ggccataggc acgggggtca catgagagcg cgacggtcgc
      181 tacgtcacca ctcgcctctt ggaaatctcc aactccagca gcgcgtcggg cagcgcctcg
      241 gacagcacac ccggctatcc atccccgatt gctaaaccgc acacgggaac gccgccggag
      301 ctcgggccgc gtcaggcgac cctcccgcac gaaacagaga ggccccggca ggagcctctc
      361 gcatcgctga acaagctgga gcccacaccc ggatttgaac cggggacctc gttcttacca
      421 agaacgcgct ctgccggctg agctatgtgg gcccgaatcg gtaccgctgt ggcggcagcc
      481 tgttcggtgg gcagggacgg attcgaaccg ccgtagctct cgcacctggt ttacagccag
      541 gcccttttaa ccactcaggc acctgccctc ttggctctcg gcctgctgtc cgactgcacc
      601 gcggagggga gccgacgatg ggactcgaac ccgcaacctg ctgtttacaa aacagctgct
      661 ctgccaattg agctacgtcg gcacagcaca aaaccgatgt ttcggccgcc tgaaagtata
      721 ggaacgccga tttggcgctg tcaaggaaga tgggccgccg atcttgccgg gccggactgg
      781 accgtcgtcc cggacgcccg ctcgcggacg gcttcaacac cgtatcaatc cgcgcccctg
      841 ggatcgagag catccctgag cccgtcaccg atgaaggtga acgacaaggt gatgagggcg
      901 acagcgatac tgggcgcaat cagcatccag gactgggcgg tgtaagactt cgagccatcc
      961 agaatcatct gcccccagct cgttgggaaa ggtgaatcga ggttggtgct tggccggacc
     1021 ccgacgccca gatagctgag caccgcttcg gcgatgatgg caccaggcac gatgaacgcc
     1081 gcggcgacga tgatcggccc cagcgcattg ggcaggatgt gcttcgcgat gatccgcaag
     1141 cctcccgagc cgctggtcct ggaggcttcg acgaattcct tttccttgag cgagagggtt
     1201 tcacctctta cgagacgagc cactccggtc cagttgacga tcgacaagct gatgaacagg
     1261 aggacaaggc cgttgaggag cttgccaatc gcatattccc cgaaggagat ctgcatgatg
     1321 atggcgagga gcaaggcagg aaaggcatag acgacgtcgg tcattcgcat caacaggttg
     1381 tcgacacgcc ctccacaata cccggcgatc agccccagcg gaacgccgat gaacaggatc
     1441 acgagcatgg ggataaagcc gactaccagc gacacccgtg ttccgtagac caggcggctg
     1501 aagacgtcgc gcccgatgga atccgttccg agtggataat cccaactgcc cgtccgcaag
     1561 gggttcggat cggtcagcca cgccgccttt cggtacgtgc cggcattcgc cacatccgtt
     1621 tgaaccgggt tgtgtggcac gatcaccggg gcaaagactg ccacgagcgc gaggaaaccg
     1681 agaaacagaa tgccggccat ggccattcgg ttccggcgaa ggcgccggag cgcattgctc
     1741 caaaggctcc gggattggcg catgttcgtc aggtcacgct cggactgttc accgagcggt
     1801 ccggcggaag cgggagcggt ggtattggcg actaccatga tgactcctcc ttaccgtcgc
     1861 gtccggatgc ggggatccag taatccatag gctaaatcga ctagaacgtt cgcgagtgca
     1921 atcagcaccg catagaaaat cgtgacaccc ataatgagcg aatagtcccg cttgccaatg
     1981 gccgacacga aggtcttgcc cagtcccggc gcgttgaaga tggtctcgat aatgatcgat
     2041 cccgtgatga ggtcagccgc cgccgggcca ataatcgtga ccactggcag caacgcgttg
     2101 cggagaatat gcctgagtac gacaggactt tcgccgacgc ccttggctcg cgcggtccgt
     2161 acgaaatcct ggcgtttgac ctcgaggacg cttgaccgtg tcagtcgggc gatgaacgcc
     2221 gttgtaccaa ggccgagaat aatacctggc aagaggtacg ctgtggaaaa gagcccctgc
     2281 cacgcctctg ggcgcttgat tggcgaaacc ccaaatgttt ggctgagaaa gatcaggaca
     2341 aggagtccgc tgacaaatgt gggcaccgaa ataccgagcg tcgagacgcc cagactgatg
     2401 taatccaccc acgtgttttg acgaagtgcg ctgatcaccc cgagtggcag cccgatgatg
     2461 agggcgaata tcactccgac caaaccgatt ctcagggaca ccgggaactg acgaacgatg
     2521 acttcctgaa cggtctccga gccacgggac tcgtatgttg gtccaaagtc gagggtcagg
     2581 atccggccca tatagttgaa gaactggctg tcgaggaaac cgcgtgcgag gctgaatgga
     2641 tttcggaccc cttggtccca tcggtcctcg aactctacgg gattagtcca gagcggtttg
     2701 tccagtccga accgggcgtt cagggtcctc tgcgcggctt ccggcatcgg cttttcgaga
     2761 tcgaatgggc cacccggggc ctggtacatc aggaagaacg tgaccgccgc aaccactacg
     2821 accaccggca cgatccacaa taatcggcga atcacaaatt gaagcatgtt ggcctctggt
     2881 gaaccactgg cggttttagt caatcattcg gggagcagcc gcgacacgaa agtgtcg
//
LOCUS       DASYWM010000318         6417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_505341, whole genome shotgun sequence.
ACCESSION   DASYWM010000318 DASYWM010000000
VERSION     DASYWM010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505341"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VGR29_12555"
     CDS             <1..652
                     /locus_tag="VGR29_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEV2074457.1"
                     /translation="NERVRGNIANALAVTAAAIGAGVPLACIRDSLRTFSTAFWQTPG
                     RFNLMMIEGKQVVVDYCHNVDALQWIADFVERTAVPQSVGVIAIAGDRRDEDIHEFGE
                     LAGRVFDRIIIRKHGDLRGRVEGEVAGLLRDAALAAGLPPERITVVLDEIEAVHTAID
                     LATPGDLVAAMVYQIPKVWEALAKRQEQPMVPSGGLPSPPEMLHVDGSLRESTARL"
     gene            1018..1674
                     /locus_tag="VGR29_12560"
     CDS             1018..1674
                     /locus_tag="VGR29_12560"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV2074458.1"
                     /translation="MRVRRALGSFLVIFAALGESASAEHSPHFDAECPTGTAQIVLDP
                     GHGGPDPGAVDEQIGLYERDVALEIATRAAEILREQGYTVALTRDDNATELGNSERGE
                     IANACQALVLVEIHLNGSTDPEINYSQAFWGEKEKDLALSLTMSNALTSLHIPLAGVD
                     RFDNGGLLRAKMPSVLVEAVFLTNPDEAKKLANGTGTRQDEIAQAIANGIAAWMELQD
                     "
     gene            1710..2477
                     /locus_tag="VGR29_12565"
     CDS             1710..2477
                     /locus_tag="VGR29_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074459.1"
                     /translation="MTTSPNRNRIAILIALMLVGGIFVAISVQQSGREQRVPITYPPG
                     AVVHGGTYGEWIARHWQWIASIPLDTNPGYDVTGQSCGDGQTGPVFFLPRNLPPCKVP
                     AGMAILVPIAGSECSSTELPPFSGTNEEELRFCAAEEVDRYTNIRLSVDGRDVPEIEA
                     YRTSSPLFALSLPDHNILGAPTGVAYAVADGYQAILRPLPPGEHEILIHLELKDGTVL
                     PDKVVRLKVVEPTWSAPAATPDIATPMVTPVATQVRR"
     gene            2617..3096
                     /locus_tag="VGR29_12570"
     CDS             2617..3096
                     /locus_tag="VGR29_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV2074460.1"
                     /translation="MAPPAPPLRININEQIHEVAASPDTPLLHVLRNDLNLNAAKYGC
                     GLGRCGTCTVHLDGEAVHACEVPVGDVGHREITTLEGLGRVGELHPLQRAFLDEQAAQ
                     CGYCIPGIIMTAAALLERNPRPSDVELRAALEGKLCRCGSHTRILRAVHRVIDSDQA"
     gene            3120..5264
                     /locus_tag="VGR29_12575"
     CDS             3120..5264
                     /locus_tag="VGR29_12575"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV2074461.1"
                     /translation="MDRPAYQTEQDVRTRDTVAVGSFLEVDPDGTVNVYSGKVELGTG
                     IATAFAQIVADELGVPFDRIRMVMGDTALTPDQGTTAGSKTVQIAGRLLRRAAAEARR
                     ELLDRAADWLEVAPEDLTVMDGVVRPVDGSAAAIPIGDLAGEPFSRKIPDNDEVRGAH
                     HVGLVGQSVPRVDLFAKVTGGEAYVHDIQLDGMLHGRVIRPYVRTMAGAGRIAHVDDR
                     QAKVMPGVVAIVRNGDFLGVVAEREEESIRAAESVQVTWEEGDRLPSREGLHELMRAQ
                     PENDTEPLHKGDVDAAMGSAARTMTATYRFPMQAHASMGPSCAVADVRGGSATIYTST
                     QGVYGLRQALAPLLGLDEDRTRLIFREGAGCYGHNGADDVTADAALLSQAVGRPVRVQ
                     WMRRDEFAWEPKGPAMLVDMGAGLDDGGAVTAWDHTVWTPTHTTRPGGQPGNLLAGQQ
                     VDPPMLPAVLRNIGGDRNAPTTYTFPSERVTMHWLNDAPLRPSAFRSLGGLHNTTANE
                     MFLDEVATATGVDPVEMRLRYLGDPRAREVVNAAAQRARWGSPLPAIEGLKSGRGFAY
                     ARYEAEYTYVAMVAEVAVDPDRGGARVTRVVVAHDCGLIVNPDGVRNQIEGNVIQGIS
                     RSLKEEVTWDNHEVTSLTWETYPILTFPEVPEIEIVLIDRPDAPSYGAGEPAICPVTA
                     AIGNAIFATTGVRLRDVPFTPERLLAAMSSGD"
     gene            complement(5466..6038)
                     /locus_tag="VGR29_12580"
     CDS             complement(5466..6038)
                     /locus_tag="VGR29_12580"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV2074462.1"
                     /translation="MVSGTGGGGLNCRTAPVSGSVITILPEGARIEVVASSQNGWAQV
                     RCASQVGWASAAYLVFNVGSGSGSGAVWIDVNLTKQYMIVYQGSNIIGQTYVSTGRYG
                     FDTPPGTFYVNYKLPSQTMTGVLGGEYYYVEDVPWVMYFTDRGHAIHGAYWHNNFGYR
                     MSHGCVNLPVGFAAWLYSISPVGTRVYIHY"
     gene            6211..>6417
                     /locus_tag="VGR29_12585"
     CDS             6211..>6417
                     /locus_tag="VGR29_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074463.1"
                     /translation="MTAPETGAVRQFSPPPPVPVTVAVDAVPDTGGVVGAGAGLVEDV
                     ETVAYVADTQPSIPTQRTGSQPLLT"
BASE COUNT         1301 a         1947 c         2003 g         1166 t
ORIGIN      
        1 caacgagcgg gtgcggggta acattgccaa cgcgctggcc gtcaccgctg ccgcgattgg
       61 cgctggtgta ccgctcgcct gcattcggga ttcgttgcga accttcagca ccgcattttg
      121 gcaaacgcca ggccgcttca acctgatgat gattgaaggc aaacaggttg tggtcgacta
      181 ctgccacaat gtggatgcac tccaatggat cgccgacttt gtagagcgaa cagcggtccc
      241 gcagtccgtc ggcgtcatcg ccattgccgg ggaccggcgc gacgaggata tccacgaatt
      301 cggagaactg gcgggacgcg tgttcgatcg cattattatc cgcaagcatg gggacctcag
      361 gggtcgggtg gaaggcgagg tggcggggct gcttcgcgat gctgctcttg cggctggtct
      421 tccaccagag cgcatcacgg ttgtgctgga cgagatagag gccgttcata ccgcgatcga
      481 tctcgcgacg ccgggcgacc tggtcgctgc aatggtgtac cagattccca aggtgtggga
      541 agccctcgcc aaacgccagg agcaacctat ggtgccgtca ggagggcttc cgtctccccc
      601 ggagatgctc catgtcgatg gatcgcttcg ggagtcaacc gctcgtctat aacgacaaac
      661 ggcatgggcg atctccagtg ccaataatgt gattcccgct cgcacagcgc tagcgttgat
      721 gcctctactg cttgagcatt tagaagcggc gaaaccactc gatagcctaa ggaactctgc
      781 atccacgctg aagattccgt taaagatgaa ttctcgcagt gtatctggga ccgtggcgag
      841 gaatctcgct gtcatcgagc tccttcgctt tagccctgaa cctgctgaca cccaatcacc
      901 atgcaatctg ctatcgcccc cgcaacggca gttgtgtatg atttcgccat gcaatcaatt
      961 aggccaaacc accggacctt tcgcatgttc gtagtgacag ggagacgggg acacgtcatg
     1021 agggtgcggc gcgcgctggg ctcgttcctg gtaatcttcg ctgctctggg agagagcgcc
     1081 tcagccgagc acagcccgca cttcgatgct gaatgcccga cagggaccgc tcagatcgtg
     1141 ctggacccgg gccacggcgg acctgacccg ggtgccgtcg acgagcagat tgggttgtat
     1201 gaaagagatg ttgctctcga gattgcaaca cgggctgccg agatcctgag ggagcagggg
     1261 tacacggttg cccttacccg ggacgataac gccacggagt tagggaactc ggagcgaggc
     1321 gagatcgcga acgcgtgtca ggcactcgtc ctcgttgaga ttcatctcaa cggctcgacc
     1381 gaccccgaga tcaactattc ccaagccttt tggggcgaaa aagagaagga tctggctctt
     1441 agcctgacca tgagcaatgc gctcacatcg cttcacattc ctctcgctgg cgtcgatcgc
     1501 ttcgacaacg gtggcttgct ccgggcgaag atgccgagcg tgctggtaga agccgtcttc
     1561 ctgacaaatc cggacgaggc gaagaaactg gccaacggca ctggtacgcg tcaggacgaa
     1621 atcgcacagg caattgcgaa cggcatcgcc gcctggatgg aactgcagga ctaacgttta
     1681 ctccacagaa cgaacttacc gagggatcca tgacaacgtc gccgaaccgc aaccgcatcg
     1741 ccatattgat cgcactgatg ctggtcggcg gaatattcgt tgccatctct gtccaacagt
     1801 cagggcgaga gcaaagggtc ccgatcacct atccgcccgg tgccgttgtc cacggtggca
     1861 cgtacggcga atggattgcc agacactggc agtggatagc cagcatccct ttggacacta
     1921 atcccggata cgatgtaaca ggccaaagct gcggagacgg gcaaaccggg ccagtctttt
     1981 tcctgccccg taacctacca ccttgcaaag tcccggccgg gatggcgatt ctggtgccca
     2041 ttgcaggcag tgagtgctcc tcgacagaac tgcctccgtt ctctggtacg aacgaggagg
     2101 agctccgctt ctgcgcggcg gaggaagtcg accggtacac caatatccgg ctgagcgtag
     2161 acggtcggga cgtacccgaa attgaggcgt accggacatc atcaccactc ttcgccttgt
     2221 cgctcccgga tcacaacatc cttggggcac cgaccggcgt cgcatatgca gtggcggacg
     2281 ggtatcaggc catcctcaga cccctgccac ctggcgaaca cgagatcctg attcaccttg
     2341 agctgaagga cggcacggtc ctgcctgaca aagtggtccg cctcaaggtg gtggaaccga
     2401 catggtcggc tccggccgca actccagata tcgcgactcc gatggtgacg ccagtggcaa
     2461 cacaggtcag gagataaccg accgccttgc ggcatccgac tacatcaccg aattccgact
     2521 accggcccgc aggcgatttc ctgtcccaaa cccgcacagg tagctgccca tctagcatgg
     2581 gcgttcacaa gcacaatcag gatcggagag cagcgtatgg caccaccagc cccgccactg
     2641 cgaatcaaca tcaacgaaca gatacatgaa gttgcggcgt cgccggacac gccgctgctc
     2701 cacgtgctgc gcaatgatct caatctcaat gcggcgaagt atggatgcgg gctgggtcgc
     2761 tgcggcacct gtactgtgca cctcgacggc gaggctgtgc acgcctgcga agtgccggtc
     2821 ggcgacgtgg ggcaccgcga gataacgacg ctcgagggtc tgggcagggt gggggagctg
     2881 cacccgctac agcgggcgtt cctcgacgag caagcggccc agtgcgggta ctgcatccct
     2941 ggaatcatca tgacggccgc tgcgctgctg gagaggaacc cacgtccgtc ggacgtagag
     3001 cttcgcgcgg cgctggaagg gaagctgtgc cgctgtggct cgcatacccg catcttacgg
     3061 gcggtccatc gagtgataga tagcgaccag gcgtagcgac gaccgggaag gacagtacca
     3121 tggaccggcc agcctaccag acggagcagg atgtccgcac gcgcgacaca gtggcagttg
     3181 gctccttcct tgaagttgat ccagacggca ccgtgaacgt ctattctggc aaggtcgagc
     3241 tgggtaccgg catcgcgacc gcctttgccc agattgtcgc ggacgagctc ggcgtgccgt
     3301 tcgaccgcat tcgcatggtt atgggcgata cggcgctaac tccggatcag ggcaccaccg
     3361 cgggctccaa gacggtccag atcgccggtc ggctcctgcg acgggcagcg gccgaggccc
     3421 gccgggaact cctggaccgc gccgccgact ggcttgaggt ggcgcctgaa gacctcacgg
     3481 tcatggacgg cgtcgtgcga ccggtcgatg gctcagccgc ggcgatcccg atcggcgacc
     3541 ttgccggtga gccattcagc cgaaagattc ccgacaatga tgaggtgcgg ggagcgcatc
     3601 atgtcggtct tgttgggcaa tccgtgccgc gcgtcgatct ctttgccaag gtaacgggtg
     3661 gcgaggccta tgtacacgac atccagcttg acggcatgct gcacggccgg gtgatccggc
     3721 catatgtgcg cacgatggct ggcgccggtc gcatcgccca tgttgacgac cgccaggcga
     3781 aggtaatgcc tggagtcgtg gcgatcgttc gcaatggaga tttcctaggc gtggttgccg
     3841 aacgagagga ggagtcaatc cgtgcagccg aatccgtgca agtgacttgg gaggagggcg
     3901 accgtcttcc gtcccgggaa ggactgcacg aactgatgcg ggctcagccg gaaaacgaca
     3961 cggagccgct gcacaaggga gatgtggatg cagcgatggg atcggcggcg cgcaccatga
     4021 cagcgaccta ccggttcccg atgcaggctc atgcctccat gggtccctcc tgcgccgtcg
     4081 ccgacgtgcg ggggggcagc gccacgatct acaccagcac gcagggtgtc tacggcctcc
     4141 gccaggccct ggcacccctg ctaggtttgg atgaggatcg gacccggctc attttccggg
     4201 agggggcagg gtgctacggg cacaacggcg cggacgatgt cactgccgat gccgcgctgc
     4261 tctcacaggc ggtcggcaga cccgtgcgcg tgcaatggat gcgcagggac gagttcgcgt
     4321 gggagccgaa gggtccggcg atgctcgtcg acatgggagc aggattggat gacggaggcg
     4381 cggtcaccgc atgggaccat accgtctgga cgccgacgca tacaacccgc cccggggggc
     4441 agccgggcaa cctccttgcg ggtcagcagg tggatccgcc gatgttaccg gcggtgctcc
     4501 gaaatatcgg gggtgaccgc aacgcgccta ctacctatac ctttccaagc gaacgcgtca
     4561 ccatgcactg gctcaacgat gcgccactgc gcccgtcggc gttcaggagc ctcggtgggc
     4621 ttcacaatac gaccgcgaat gagatgttcc tcgacgaagt tgcaactgcg accggcgtcg
     4681 acccggtcga gatgcggttg cgctatctgg gcgatccccg ggcacgcgaa gtggtcaatg
     4741 ctgctgccca gcgcgcccgc tgggggtcgc cactgccggc catcgagggt ttaaagtcag
     4801 gcaggggctt cgcctacgcg cgctatgaag cggaatacac ctatgtcgcc atggtcgcag
     4861 aggtcgcggt cgatccagac cgaggtggag cgcgggtcac gcgagtagta gtcgcccacg
     4921 actgcgggct gatcgtcaac ccggacggtg tcaggaacca gatcgaaggc aacgtcattc
     4981 agggtatcag ccggtccctc aaggaggagg tcacctggga caaccatgag gtgacgagtt
     5041 tgacgtggga aacctatcca atcctgactt tccctgaggt gccggaaatc gagatcgtgc
     5101 tcatcgaccg gcccgatgca ccatcctacg gagccggcga gccagcgatc tgcccagtca
     5161 ccgctgcaat cggcaacgcc atctttgcca ccactggcgt gcggttgcgg gacgtaccgt
     5221 tcacgcctga acgtctcctt gcggcgatga gctctggaga ctagcgctct attgcacaac
     5281 caggtgcttg gcccagcaat ctcactcggt acaaaagggg ccgggtagcg cactccttgc
     5341 aatacgagtc ctgaagcaga tagctccagc ggttacggct gttcggagcc aaatgccgtc
     5401 atgaacctcg atgagggagc ggagatgcat ctccggtccc tcatctggat caccaagtgt
     5461 acttgctagt agtgaatgta gacccgtgta cccacgggcg agatgctata cagccacgct
     5521 gcaaacccga cgggcaggtt gacgcatcca tgactcatcc ggtaaccgaa gttattgtgc
     5581 caataggcac catggatggc atgaccgcgg tccgtgaagt acatcaccca cggtacgtcc
     5641 tcgacgtagt aatactcgcc gcccaacacc ccggtcatcg tttgggacgg caacttgtag
     5701 ttgacgtaga acgtgcctgg tggggtatcg aagccgtacc ggccggtact gacataggtt
     5761 tgaccaatga tgttgctacc ctggtacacg atcatgtatt gcttcgtcag gttgacatcg
     5821 atccagaccg cgccggagcc gctgccactt cctacattga agacaaggta ggcggcagaa
     5881 gcccacccca cctgactggc gcagcgcacc tgcgcccacc cgttctgtga cgacgccacg
     5941 acctcgatcc gtgcgccttc cggaagaata gtgataaccg agcccgaaac cggcgccgta
     6001 cgacagttca atccgccacc gccggtgcca gataccatgg catagctgga gctacccgac
     6061 ggcgcagtcg gtgtcggatt cgtgcctgat gtggaaagcg tgacgtaggt tgcggagacc
     6121 cacgcgtcct gcccactgca acgaaccggc agccagccgt tcgtgacggc gccacgggtc
     6181 tctaccagtg cgcctttcgg aagaatggtg atgactgcac ctgaaaccgg cgctgtgcgg
     6241 cagttcagcc cacccccgcc agttccagta acggttgcag tggacgcggt gccagacact
     6301 ggcggcgttg tcggagccgg cgccgggttg gttgaagatg ttgagacggt ggcgtacgtg
     6361 gcggataccc agccgtccat cccgacgcag cggacaggca gccagccatt gctgacg
//
LOCUS       DASYWM010000319         7701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_355667, whole genome shotgun sequence.
ACCESSION   DASYWM010000319 DASYWM010000000
VERSION     DASYWM010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355667"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VGR29_12590"
     CDS             <1..1224
                     /locus_tag="VGR29_12590"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV2074464.1"
                     /translation="VAVMYRTTAQSRALEEAFRTSEFRYRIVGGVRFYERKEVRDILA
                     VLRLVYNPSDNVSLQRVIDNFPIGRGFGPRALEVAVDWAAEHRRSTLDAFLALVPSVA
                     ARADVPAPSFSGAGKTAAQRIGTTFAILGEHQKTHSLAELFDSIIDVTEFRKSFDGGT
                     EEELQRWANVQELRNDLERYDNVDPEEALPNYLEQVALISDADTMADDTQGTVTLITL
                     HSAKGLEFPVVFIAGAEEGLLPINRAVEAEAFDPAPMEEERRLFYVGITRAQKLLYIT
                     YAANRMTYGQYRDGVVSRFVQSIPAEAVRALTRETAARSSNTGRSYGKTSPGSLASKV
                     TSSWSMPEPVAKKVPEALPDYKEGNQVFHPKFGEGQITEITSRKNGDMEISVAFKRHG
                     QKRLIASLANMDILS"
     gene            1238..2287
                     /locus_tag="VGR29_12595"
     CDS             1238..2287
                     /locus_tag="VGR29_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEV2074465.1"
                     /translation="MNGTSGESIRERLERQEDALLVARACRSAGASRVHPEPPSEMRT
                     EFQRDRDRIIHTKSFRRLMHKTQVFIGPKGDHYRTRLTHTLEVMQIGRTIGRALGLNE
                     DLIEAIGMGHDLGHTPFGHAGERALAEVFPGFRHNEQSLRIVDTLENDGRGLNLTLPV
                     RDGILRHSKARASIMQSHASVPTSLEGHVLKIADGVAYMNHDLDDAIRAGIVSIEEVP
                     AEVTRVLGNRHSQRINTLVADIVNHSDVALPDGEIVMSGGIHEAADVLRDFLYERVYD
                     PINTRPETLHARDIVRTHYHYFLDNPDGMTEPYVVAVDNEPVERLVADFVASMTDRFA
                     TELYERLHAPSVMAP"
     gene            2384..4267
                     /gene="dnaG"
                     /locus_tag="VGR29_12600"
     CDS             2384..4267
                     /gene="dnaG"
                     /locus_tag="VGR29_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV2074466.1"
                     /translation="MANDAVTEVRDRIDVVDLVQGYVPSLRKTGSSFKGLCPFHNEKT
                     PSFIVFPDSQNFHCFGCGKGGDIFTFYMLVENVEFREALKELANRAGVELEVGAPQLP
                     EEDEKRRKLIEVNELVGMWFHHVLKATRHGEPGRQVVTDRGISDEMVEQFRLGFAPDS
                     WDALLNFLASRNISAELAAEAGLASKRDSGGYYDRFRNRLIFPIRDRDANIVGFGGRA
                     LGDAQPKYLNSPQTLIFDKSSLVYALDVAKDAIRAANEVVIVEGYMDAITAHQFGFNN
                     VVASMGTALTEQQVAQIKRGSSRIVLALDSDAAGQMATLRGLETMADALDAEVRPVPT
                     PNGLLRFERKLKAEISIVQLAEGKDPDEIIRKSPEKWPELIRTARPFLDFTIDMLTRD
                     VDTSNPEAKSDAVRRIIPLLRQIPDGVKENHYTRMLARRLDISEQVIVSEKRKLSIGG
                     KSAAIRAGLKPTAIEKVRRRSTEDYLVALLLRHHQLTHDILVRIPEEDIQDARNREII
                     ALLRDPSTNELEGRDLTVAMDDELADHAEHLLDTLEGRPEQFPSHIQTEARQVLETLG
                     RERFSFLMRQLHHSLQSAQQDQDGVAIQELREQIVKLSKRHAEHYPSPSPYFQDSRSE
                     RKF"
     gene            complement(4353..5117)
                     /locus_tag="VGR29_12605"
     CDS             complement(4353..5117)
                     /locus_tag="VGR29_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HEV2074467.1"
                     /translation="MGVFGSVAVISDIHGNLPALDSVLSEPLVANADALVVAGDHASG
                     PQPTEVLDRLRSLGDRAFLVAGNADREMVNIARGRDVPIPDEVTAWAASRLSSEHVTM
                     LEVLPHPLVVNIAGFGQVLCCHGSPRRIDDVVLVDSGLQRWQEVFQNVDPNIKTVVCG
                     HTHMPFVRLASGRLVINPGSVGMPYGRKDPSWAILSQRKVTLGSTGVDSDDVARRISE
                     SSRFPGVNHWIRDYVLGRPSDVDVIDAFGPMDGRST"
     gene            complement(5297..5373)
                     /locus_tag="VGR29_12610"
     tRNA            complement(5297..5373)
                     /locus_tag="VGR29_12610"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5337..5339),aa:Pro,seq:cgg)
     gene            5773..5848
                     /locus_tag="VGR29_12615"
     tRNA            5773..5848
                     /locus_tag="VGR29_12615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5806..5808,aa:Arg,seq:cct)
     gene            5994..6575
                     /gene="raiA"
                     /locus_tag="VGR29_12620"
     CDS             5994..6575
                     /gene="raiA"
                     /locus_tag="VGR29_12620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV2074468.1"
                     /translation="MELQIRANGTKVTDGMREFIDRRMTKLDRLADRVIDAQLELRTE
                     TTRTEAEITTAQLTLRTRKHVLRAEAEHVEAARAIDMVIDKLITQVRRYNDKRTSRKR
                     RGGRGEFAATAATETALPRDPDFTDMPDGVTDEFEVARTKSFQMDPMDVETAIEQMEL
                     IGHDFFFFKNEEDEKFSVLYRRRDGTYGILAPE"
     gene            complement(6649..6987)
                     /locus_tag="VGR29_12625"
     CDS             complement(6649..6987)
                     /locus_tag="VGR29_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074469.1"
                     /translation="MNVQEGLGEVRRRLVENKAQPESIKVVDAILQRASLPAASGASA
                     TSLLQLVRMLMRTPVAASKPSVYNDFVRIEADLEVQAEEIRTIREEEDRRPIPKTKKY
                     YKDIKKKGEK"
     gene            7121..>7701
                     /gene="thiD"
                     /locus_tag="VGR29_12630"
     CDS             7121..>7701
                     /gene="thiD"
                     /locus_tag="VGR29_12630"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV2074470.1"
                     /translation="MTSQPLYKALTIAGSDSGGGAGIQADIKTFASLGLYGLSVVTAV
                     TAQNTVEVAAIAEVPEEVVIAQIDTVLEDIGAHAAKTGMLSSIPIIQNTADRLEAWGV
                     PYLVVDPVMVSKSGAPLLQPEAVASLVKDLLPLATVVTPNVAEAEVLSNRQIRSPEDA
                     LEAARAIHALGPQVVVITGGHMEGPANDLVFSG"
BASE COUNT         1598 a         2362 c         2295 g         1446 t
ORIGIN      
        1 gtcgctgtga tgtaccggac cacggcgcag agccgcgcgc tggaggaagc gttccgcacc
       61 agcgagttcc gctacaggat cgtcggcgga gttcgcttct acgagcgcaa ggaagtgcga
      121 gacatccttg cggtcctgag actggtgtac aacccctccg acaacgtcag cctccagcgc
      181 gtgatcgaca acttccccat cggaaggggc tttggtccac gtgcgctcga ggtcgcggtc
      241 gactgggccg ccgaacaccg gcggtcgaca cttgacgcct ttctggctct cgtcccctct
      301 gtcgccgcca gggccgacgt cccggcaccc tcgttctccg gcgcggggaa gaccgccgct
      361 cagcgaatcg gcacaacatt cgccattctt ggggagcacc agaagacgca tagtctcgcg
      421 gaactctttg actcgatcat cgatgtcacg gagttccgaa agtccttcga cggaggaact
      481 gaggaagaat tgcagcgctg ggccaatgtc caggagcttc gcaacgacct tgagcgatat
      541 gacaacgtcg accccgagga ggcactgccc aactacctgg agcaagtcgc gctcatttcg
      601 gatgctgaca caatggcgga tgacacccag ggcaccgtca cgttgatcac attgcattcg
      661 gccaagggtc tcgagttccc ggttgtcttc attgcggggg cggaggaagg actgctgccg
      721 atcaaccgag cggtggaagc tgaagcgttc gatcccgctc ccatggagga agagcgccgc
      781 ctcttctacg tcggcattac ccgcgcccag aagttgctct acatcaccta tgcggccaat
      841 cgaatgacct atggccagta cagggatggc gtcgtctcca gatttgtcca gagcatccct
      901 gcggaagccg tgcgggcgct gacacgcgag accgcggccc gatccagcaa cactggccgg
      961 agttatggga agacatcccc agggtcgttg gcctctaaag tcacatcctc gtggtcgatg
     1021 cctgaacccg tggcgaagaa ggtgcccgag gcgctccccg actacaagga aggaaaccag
     1081 gttttccatc caaagttcgg cgagggacaa atcacagaga tcacttcccg caagaacggc
     1141 gacatggaga tctccgttgc cttcaagcgc cacggtcaga agcgactgat cgcctcgctg
     1201 gcgaatatgg acattctttc ctgacggagg catcggtatg aacggtacct caggagagtc
     1261 gatccgggaa aggcttgagc gccaggagga cgcgctgctg gtggcccgcg cgtgccggag
     1321 cgccggtgca agccgcgttc atcccgagcc tccgtcggag atgcgaaccg aattccagcg
     1381 ggatcgggac cggatcatcc ataccaagag cttccggcgg ctgatgcaca agacccaggt
     1441 cttcatcggg ccaaaggggg atcactaccg cacccggttg acacatacgc tcgaggtcat
     1501 gcagatcggc cgaacgattg gacgggcgct cggccttaac gaagatctga tcgaggcgat
     1561 cgggatgggc catgacctcg gccatacccc gttcggccac gcgggcgaac gggcccttgc
     1621 cgaagtcttt cccgggtttc ggcacaatga gcaaagcctc cgtatcgtgg ataccctgga
     1681 gaacgatggc cgtggcttga acctgaccct ccccgtgagg gacggcatcc ttcgccattc
     1741 caaggcacga gcatcgatta tgcagagcca cgcatcggtc ccgacatccc tcgaggggca
     1801 tgtcctcaag atcgccgatg gcgttgccta catgaaccac gacctcgacg acgcaatccg
     1861 ggcgggcatc gtgtcaatcg aagaggtccc ggcagaagtg acgcgggtgc tcgggaacag
     1921 gcactcgcag cgaatcaata cccttgtcgc cgacatcgtg aatcactctg atgtggcatt
     1981 gcccgatggt gagatagtca tgtccggcgg catccacgaa gcagcagacg tcctgcgtga
     2041 cttcctctat gaacgcgtgt acgatcccat caacacacgt cctgaaaccc tccacgcgcg
     2101 ggatatcgta cgtacgcact accattactt cctggacaat ccagacggaa tgaccgaacc
     2161 ctatgttgtt gccgtcgaca atgagcctgt cgagaggctg gtggctgact tcgtcgcctc
     2221 gatgaccgac cgtttcgcta cggagttgta cgagcggcta cacgctcctt ccgtgatggc
     2281 accctagcgg ggagtgacca ggggaaccgg tcatccatcc cacgacaatt agtgaccgca
     2341 acgtccgcgt gaccagcacc accgctccag acaggaaccg ccaatggcca acgatgctgt
     2401 aaccgaggtt agagaccgta tcgatgtcgt cgatctcgtg caggggtacg tgccgtcgct
     2461 gagaaagacc ggctcgagct tcaagggcct ctgccccttc cataacgaaa agacgccatc
     2521 cttcatcgtg tttcctgact cccagaattt ccactgcttc ggctgcggaa agggaggcga
     2581 catctttacc ttctacatgc tggtggaaaa cgtcgagttc cgggaagccc tcaaggagct
     2641 ggcgaaccgc gcgggcgtcg agctcgaggt gggggcgccg cagttgcccg aggaggacga
     2701 aaagcggcga aagctgatcg aggtgaacga gctggtcggt atgtggttcc accatgtact
     2761 taaggcaacc cgccatggag agcccgggcg tcaggtcgtg acggaccgcg gtatctccga
     2821 tgagatggtt gagcagttcc ggctggggtt tgctcccgat tcgtgggacg ccctgctcaa
     2881 tttcctcgcc tcgcggaata tctccgcgga gcttgccgcc gaagcgggat tggcaagcaa
     2941 gcgtgactcg ggcggttatt acgatcggtt ccgaaaccgt ctcatcttcc ctatccgtga
     3001 tcgcgatgcc aatatcgttg gcttcggtgg acgagcgctc ggtgacgctc agccgaaata
     3061 cctcaactca ccgcagaccc tgatctttga caagagctcc ctcgtctatg cgctggatgt
     3121 cgcaaaggac gcgattcgcg ctgcgaatga ggtggtcatc gtcgaggggt acatggacgc
     3181 gatcactgcc caccaattcg gtttcaacaa tgttgtcgcg tctatgggta ccgcactcac
     3241 ggagcagcag gtggcccaga tcaagcgggg gagcagccgc atcgtactcg cgctggattc
     3301 cgacgcagcc ggacagatgg ccaccctgcg cggactcgag acgatggcgg atgctctcga
     3361 tgccgaagtg cggccggtgc cgacgcccaa cggcctcctc cgttttgagc gaaaactcaa
     3421 ggctgagata tcgatcgtcc agctcgccga gggtaaggat ccggacgaga tcatccgcaa
     3481 gtcaccggag aagtggccgg aactgatccg cactgcccgt cccttcctcg acttcacgat
     3541 cgatatgctc actcgtgatg ttgacacctc taaccccgag gcaaaatccg atgctgttcg
     3601 gcggatcatt cctctgctgc gccaaattcc cgacggcgtc aaggagaacc attacacccg
     3661 catgctggcg cggcggctcg atatctcgga gcaggtcatc gtctctgaaa agcgcaagct
     3721 cagcatcggg ggtaagtcgg ccgcgattcg cgctggcctg aagccgaccg ctattgagaa
     3781 agttcgccgc cgatcaaccg aggactacct tgtggcgctt ctcctccgcc atcaccagct
     3841 cacgcatgat atcctcgtgc ggattcctga ggaggatatc caggatgcaa ggaaccggga
     3901 gattatcgcg ctgctccgcg atccatcaac gaatgagctc gaggggcggg atctgacggt
     3961 ggcgatggac gacgagctcg ccgaccacgc agagcacctg ctggataccc ttgagggccg
     4021 tcccgagcag ttcccgagcc atatccagac cgaggcgcgt caggtgctgg agacgttggg
     4081 ccgggaacgg ttctccttcc tcatgcggca gctgcatcac tcccttcagt cggcacagca
     4141 ggatcaggat ggagtggcta tccaggagct gcgggaacag atcgtgaagt tatccaaacg
     4201 gcacgcggaa cattacccca gccccagccc ctactttcag gacagcaggt ccgagcgaaa
     4261 gttttagggc cttggttccg gttcctgctt cccatgcgga ttgaattgag cctgcgggat
     4321 cagctcctga tgcgtcgctc gcgcaaggag ggtcaggtgc tacgtccgtc catcggccca
     4381 aacgcgtcaa tcacatcgac atcagacggc cttcccagga cgtagtcccg gatccagtgg
     4441 ttcacaccgg gaaaccggga tgattcggaa atacggcgag ccacgtcgtc cgagtccacc
     4501 cccgtggacc cgagtgtgac tttcctctgg gacagaatcg cccatgaggg atccttcctg
     4561 ccgtaaggca tgccaacgct tccgggattg atcacgagcc tgccagaggc caggcgcacg
     4621 aacggcatat gtgtatgccc acagacaacg gtcttgatgt tgggatcgac attctgaaac
     4681 acctcctgcc aacgctgaag accggaatcc acgaggacga catcgtcgat ccgccgggga
     4741 cttccgtggc agcaaagtac ctgaccaaag cctgcgatgt tcacgaccag cgggtgaggc
     4801 aggacctcca gcattgtgac gtgctcgctg gagagacggg aagcagccca agcggtgacc
     4861 tcgtcgggaa tcggtacatc ccttccccgc gctatgttca ccatctcgcg atcagcgttc
     4921 cctgcgacga ggaatgcccg gtcgccgagc gagcgcagac ggtccagaac ctcggtgggc
     4981 tggggccccg acgcgtgatc tcccgcgaca accagggcat cggcgttggc gaccagcggc
     5041 tcggagagca cggaatccag tgccggaagg ttaccgtgga tatcagagat gaccgcgacg
     5101 ctaccaaaga ctcccattgt caccgccatc cttcaccctt ggcatgggac ctgagcagcc
     5161 aatcgttcag cgggagcaag gtcctgccga aacgcggccg ctggacatat acgcgccaat
     5221 ttctggtcca agaccagggc aggaaacaga aaactcccct gcacttcagc gtgcagaaga
     5281 gttgattccc ggtgtctggt cggggcgaga ggatttgaac ctccgacctc agcgtcccga
     5341 acgctgcgcg ctgccggact gcgccacgcc ccgtgacgac tgcaggagaa taccacgtag
     5401 acgcctggag caacaagacg gcccttgccg gccacctcac atgtgtcccg gcgcgaactt
     5461 cacactgaag ctgttagcat tgtgcaggca gggcacagcc tcgtgacccg cggctgtcga
     5521 tctgctccat ctctccacaa caacgacagg gatggtgtcg gaactatttt gaggacggac
     5581 ggtcaaagcc ccctgaaaac ggctcttctt ggcggagagt tgtatgcaac aacatcgacg
     5641 gagtaaggca ccggctcgct tcgtccagcc aagacggctg gatgctgtct tctcgaggcg
     5701 accctgtcct atccccttgc cgaatggacc gggaggtgtg gtagcatcgc agcagcgagc
     5761 gagtagcttc ctgcccgtgt agctcagcgg atagagcgtc accgtcctaa ggtgagcgtc
     5821 gggggttcga gtccctccgc gggcgccact cacgtcttga tccaggtgca agccaaccac
     5881 cacgttttgg ttcgtggttc tcctctgcac ccgcgcttgt gatccatgca ttgcgtctga
     5941 catggggcac caatcgggga ccccattgga agtctcgtta cgaggaggct gcaatggaac
     6001 ttcagatccg agccaatggc accaaggtga ccgacggcat gcgggagttc attgaccggc
     6061 gaatgacgaa gctggacagg ctggccgaca gggtcatcga tgcgcaactc gagcttcgga
     6121 ctgagaccac gcggaccgag gccgagataa cgaccgcgca gctcaccctc cgaacccgca
     6181 aacacgtcct ccgcgccgag gccgagcatg tcgaggcggc cagggcaatt gatatggtca
     6241 tcgacaagct cattacccag gtccgccgtt acaacgacaa gcgaacgagc cggaagcgcc
     6301 ggggtggacg cggcgagttt gcagccaccg cggcgaccga gacagcgctt ccccgtgacc
     6361 ccgatttcac ggatatgcct gacggcgtta cggacgagtt cgaagtggcc cgcaccaagt
     6421 ccttccagat ggacccgatg gatgtcgaga cagcgatcga gcaaatggag ctgatcggcc
     6481 acgacttctt tttcttcaag aatgaagagg acgagaagtt cagcgtgctc tatcgtcgcc
     6541 gggacgggac ctacggcatc ctcgcacccg aataacgagg atcgaccctc ctcactactc
     6601 ctcaggagcg cctcccgcac tggtcagggg aggcgcttcg tgtctcctct acttttcacc
     6661 cttcttcttg atgtccttgt agtacttctt ggtttttgga atcggacgcc gatcctcttc
     6721 ctcgcgaatc gtccgtatct cctcggcctg gacttccagg tcagcctcga tccggacgaa
     6781 atcgttgtag accgaaggtt tggatgcggc gaccggggtg cgcatcagca tccttacaag
     6841 ctgcagcagt gatgtggcgg atgcgccgct ggctgcgggt agcgaagccc gttgcagaat
     6901 cgcgtcgacc accttgatag actccggttg cgccttgttc tcgaccagcc tgcgccggac
     6961 ctcaccgagg ccctcctgta cgttcatgtg gctttccttt cgactgcggg agaacccttg
     7021 tcccgggtag tatatgcagg agtagacgtg ggcgtaggta gagcgctctt tatagatagc
     7081 gcacgcctcg cgggtcccaa taatcaagga tgagctccca gtgacatctc aaccgctata
     7141 caaggcactg acgatcgccg gatcggattc cggcggtgga gccggcatcc aggcagatat
     7201 caagactttc gcgtcgcttg gactttacgg actctccgtg gtcaccgccg tcacggcaca
     7261 gaacactgtt gaggtggccg cgatcgccga agtaccagag gaagtggtca tcgcccaaat
     7321 cgacaccgtt cttgaggaca tcggtgctca cgccgcgaag acaggcatgt tgagcagcat
     7381 cccgattatc cagaacacgg cggatcggct ggaggcgtgg ggcgtgccat acctcgttgt
     7441 cgatcctgta atggtgtcga aatcgggcgc gccgcttctc cagcccgagg cagtcgcatc
     7501 gctcgtgaag gacctcttac ccctggccac ggtggtgaca cccaatgttg cggaggcgga
     7561 ggtcctttcc aaccgtcaga tccggtcacc agaggacgcg cttgaagcgg cacgggctat
     7621 tcacgccctc gggccgcagg tggtcgtcat cacaggcggc catatggagg gcccggcgaa
     7681 tgacctggtc ttcagcggcg a
//
LOCUS       DASYWM010000320         9135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_486871, whole genome shotgun sequence.
ACCESSION   DASYWM010000320 DASYWM010000000
VERSION     DASYWM010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486871"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            739..1128
                     /locus_tag="VGR29_12635"
     CDS             739..1128
                     /locus_tag="VGR29_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV2074471.1"
                     /translation="MTEINAVVSNRAPVAIGPYSQAITANGTVYTSGVIPLDPETKEI
                     DGADITTQTERVLKSLASLLEDAGSSLDQVVKTTCFLQDLNDFATFNQIYASYFNSKP
                     APARSTVQVAKLPMGVLVEIEAVALLK"
     gene            1197..1273
                     /locus_tag="VGR29_12640"
     tRNA            1197..1273
                     /locus_tag="VGR29_12640"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1231..1233,aa:Val,seq:cac)
     gene            1604..2332
                     /gene="extP"
                     /locus_tag="VGR29_12645"
     CDS             1604..2332
                     /gene="extP"
                     /locus_tag="VGR29_12645"
                     /inference="COORDINATES: protein motif:HMM:NF040969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HEV2074472.1"
                     /translation="MRREQTLADRITGSALWRSVVRHGYPDTQRNQALIIASNVFLHI
                     HPVKIKRYATKVTYTFCLGGLSFFMFLVLTMTGVLLMFYYVPSADQAYQSMLDIEGSV
                     TFGQLMRNMHRWSAQGMVIAVFLHMGRVFYTGSYKPPREFNWVIGVLLLVTTFLLSFT
                     GYLLPWDQLAYWAITVGAEIGGAAPIVGPQVSRVLLGDFQIGQSTLIRFYTLHVIFLP
                     LVAAFLMAVHFWRIRKDGGISGPL"
     gene            2381..3169
                     /locus_tag="VGR29_12650"
     CDS             2381..3169
                     /locus_tag="VGR29_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074473.1"
                     /translation="MADIATPPSGGAPAAIDEKKRQKLLELVRGRALARPTDLAEDEV
                     MVWPSLVVVEAVSAVVFTLILVVLSLLVNAPLLERANPNQTPNPSKAPWYFLNLQEML
                     LHMDAGLAGVIIPTIALGGIAAIPYLDRSPLGVGILGTSSKGRKIIAFTAVYTTVVLV
                     GMVVLDEITERGTQFGIGKALQDQGAPEWVGGIVLSSLFIIVSIAVLVGLVQAIFKPT
                     RRELIIALFTGFFVTYFVLGIFGTSFRGQGQNLTWPWDLPLVHH"
     gene            3238..3834
                     /locus_tag="VGR29_12655"
     CDS             3238..3834
                     /locus_tag="VGR29_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV2074474.1"
                     /translation="MNPLLRTVVGLGTGWVASQGVAAVAHLQSGHGQTLAKKLHSRRS
                     FTRNAALGATGIVLAQAGVAFGLLMWPNKTGAFGGELTVGADSIPEVGATPFRHQEGK
                     FYIVRTEDGIEALYWKCVHLGCTVPWIEGDELFICPCHGSVYEYNGSRIAGPATRAMD
                     LMPTEVGEDGSVTVNTSPSALIQRPEYSPDHATPYPFS"
     gene            3905..4900
                     /locus_tag="VGR29_12660"
     CDS             3905..4900
                     /locus_tag="VGR29_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEV2074475.1"
                     /translation="MGAVRKLATVVIIGLVALSTLMVVYLAAEPDRRGSETSEQQSTM
                     LVRGTELYITYCLQCHGPAGLGAAGRDGRVGGILNQDAVIPEDEQGDLNANFQSDDPV
                     ARSVAEDWIRFRIAYGVPPDPIAEAKVMPAFANDLNVEEMNALVYLIMDGDWNYVYNT
                     IVHETGVHVAEEECLENPDAEYCDDVSQAPPVYPTAPPAGGNPNAESDEATTPESADE
                     GAADDAAASEGESAAGASEGGEAAASLEAQDIAWSQTELRVKPGDTIEMTNTGQLPHD
                     FTVDELGIQEVTPTNGDTVTITIPEDAEDGEFEFYCSVPGHKEAGMVGTLIIEAP"
     gene            4919..5125
                     /locus_tag="VGR29_12665"
     CDS             4919..5125
                     /locus_tag="VGR29_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074476.1"
                     /translation="MLITNLATAFGTLAETVELALAFIMMIGGIIITHLIQRWADKTG
                     GEQPAAANVGQGGIAQYEDNTMRD"
     gene            5326..5787
                     /locus_tag="VGR29_12670"
     CDS             5326..5787
                     /locus_tag="VGR29_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074477.1"
                     /translation="MANRNSPTKTYLWCDTCRRSYRHEETNSGACPVCGAGTRELGKF
                     SAILRGLMANELSPSPLVSKHRQLVRLIWTHNGMGEQYYRVIAPEMSYNRFEAKVTEI
                     LCQGAEEGWVEFVIPPAPREEESAYQLKLVDEDRFISELQALAGPDAAIEE"
     gene            5793..6413
                     /locus_tag="VGR29_12675"
     CDS             5793..6413
                     /locus_tag="VGR29_12675"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEV2074478.1"
                     /translation="MRDHEERLEDRTIGDYLDTLASSAPAPGGGSVAGLIGALSAALG
                     EMVISLTRDTEPSLEEAAGKLRAFRSSAMASSAADEMAYSSFVEASRMSKETGEDKAR
                     RTATMQEALEEAAAVPLELAGTAIQLLEHFEPVVRLGSKHVLSDVATAITLAVACVDC
                     SLINVRVNLPMMKNIEVAGSLRGKAQKVEQQAHALAEELRDELNKR"
     gene            complement(6511..6864)
                     /locus_tag="VGR29_12680"
     CDS             complement(6511..6864)
                     /locus_tag="VGR29_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074479.1"
                     /translation="MPRIDYEQLADRRSGERSTAVRGRVEAPRELQAKRYVGSELLTN
                     ADMGPKEVHKHARLDEAGEHLVRMAVRQLSLSARAYHRVLKLARTIADLAVSDQIRSE
                     HVTEALQYRPRQAEI"
     gene            complement(6853..7383)
                     /locus_tag="VGR29_12685"
                     /pseudo
     CDS             complement(6853..7383)
                     /locus_tag="VGR29_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            7498..7836
                     /locus_tag="VGR29_12690"
     CDS             7498..7836
                     /locus_tag="VGR29_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074480.1"
                     /translation="MIVKSAVSRLTPTRSQYVRSAKLDDSVLLLIEPCIDLSECCLSR
                     SAFGFYPGDLSLDQLPRKTVIFSFGIATHKALPLLLHLVKHPCRGFTTVLEGGVWTIL
                     SAAFYFGDDR"
     gene            complement(8558..8998)
                     /locus_tag="VGR29_12695"
     CDS             complement(8558..8998)
                     /locus_tag="VGR29_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074481.1"
                     /translation="MALHARVHPRTRLLLAALLPALATAVMLIPPLFGTTGPGPGRSL
                     ALFWLLVFVVSAPMVAFVVNWHWPAATTANRAFLAALPQPVVVPLMLWLAVSLDIQRG
                     NLVRDTSEEAMTSGFATVGGLLIGLILMILVAVGGQLGARLVDR"
BASE COUNT         1894 a         2738 c         2747 g         1756 t
ORIGIN      
        1 ctgatctctc agcagcggac aagtagtata cctgcccggt ccggtccgtg tcaagcccct
       61 cccgaaagag ccgcttgcgc cggacctgct gcccggtcgt tcccggagca gcggagagca
      121 agtgtacccg ctcccccacc ccctgtcaag accctgtttc caggaacctc tctcagggtc
      181 cgcgccaccg atttctcagc agcggagagc aactatagct gcccccctgc cacctgtcaa
      241 ggctgcccca tcgacgggcc gcgcgcacca ccaggtcccc ggcagcgcag cgcaggtcta
      301 ccgatcctca acggggcgtc acggccccgc tcaggacgag ggagacggaa gatgggcacg
      361 ggccgcccac ccacctctat agggacaggc aacagggcgc gggagagggg ggatgggcgg
      421 aagagggaca caccgcgccc aactgcttct tgttcagagg ggtgccgggt tgctccatgc
      481 ttcgcagctt ggaccaatga accgttgcgt gagcacgctc gaagcaatgc gcggggtgta
      541 gtgagcaatc gcgcttttca tggggagact atgtagcctc atatggaaga accaaaacgg
      601 agcaacaaga ataaggaatt taaactacag acccgaccga acgcatccca ctcgccgggt
      661 acttcacaat ctggcacaat gcctcgactt cggctctcgt gtgccgcaat tggcggctcc
      721 catacagaaa ggttgatcat gactgagatc aatgcggtcg tctctaatag agcaccggtg
      781 gcaattggtc cctactccca ggcgatcacc gccaacggca cggtctacac atctggtgtc
      841 atcccgctag atccggagac aaaggaaatc gatggtgctg atatcactac gcagaccgag
      901 cgggtcctca aaagccttgc ttccctgctg gaggatgccg gctcatcgct tgatcaggtg
      961 gtcaagacca cctgcttcct gcaggatctg aatgacttcg cgacgttcaa ccagatctac
     1021 gcttcgtact tcaattcgaa gccggcgccg gccaggagta ccgtgcaggt cgcgaagctt
     1081 ccgatggggg tgcttgtcga gatcgaggcc gtcgcgctac tcaagtaaat agcagcctgt
     1141 gcggggttgg cgggcgtgtc aatcgacatg tatactcccc gcaccatcaa agtgatgggc
     1201 gtatagctca gctggccaga gcgcacggtt cacatccgtg aggtcacagg ttcaagtcct
     1261 gttacgccca ccaggacaaa cacaagcgtc gatgtcgaga gaagcgccac acggaagatc
     1321 cggggcgcat ttttgtgccc aggcagcaag gcacgaagca gaactcctgt attcgcgtgc
     1381 ctgaaagcgg cacctgggca caattttctt ggagttttac ttgaccgggt ataacagccc
     1441 ggaccacaat atcaacatcg attgtcccgg ctccgggccg gtagtcggtc tccgggtttc
     1501 cagtggcact atcgcgtgcc ttcctgatgc aaccgggcca gacatccgta caacaacata
     1561 tcgcggacat cgtcgttagg tcacccttgg aggaggaagt cgcatgaggc gcgagcagac
     1621 gctggccgat cggatcactg gctcagcgtt atggcggtcg gtggtgcgtc acgggtaccc
     1681 cgacacgcag cgtaaccagg cgctgatcat cgccagcaac gtgttcctcc atatccatcc
     1741 ggtcaagatc aagcgttacg cgacaaaagt gacgtacacc ttctgtctgg gcgggttgtc
     1801 cttcttcatg ttcctcgtgt tgaccatgac gggcgtcctc ctgatgttct attacgtccc
     1861 gtccgcagac caggcctacc agtcgatgct ggacattgag ggtagtgtca cctttggcca
     1921 actgatgcgg aacatgcacc gctggtccgc gcagggcatg gttatcgccg tgttccttca
     1981 tatgggacgc gtcttctata ccggctcata caagccgccg cgcgagttca actgggtgat
     2041 cggcgttctt ctgctcgtga ccaccttcct gctttccttt accggctatc ttctcccgtg
     2101 ggaccaactc gcctactggg cgatcaccgt cggcgcggaa attggtggtg cggctccgat
     2161 cgtcggcccg caagtgagcc gggtgctgct tggtgacttc cagattggcc agagcacgct
     2221 catccgcttc tacacattgc acgtcatctt cctcccgctc gttgccgcct tcctgatggc
     2281 cgttcacttc tggcgcatcc gcaaggacgg cggtatctct ggcccgctgt aggcccgaat
     2341 tcttctactg gtagctcagt gatctgaagg aaggaatcgc atggcagata tcgcaacacc
     2401 gccctcggga ggcgcaccag cggccatcga tgaaaagaag cggcagaagc tccttgagct
     2461 cgttcgcggt cgcgcccttg cccgaccgac cgacctcgcg gaggacgaag tgatggtgtg
     2521 gccatcactt gtcgtcgtcg aggcggtgag tgcggtcgtc ttcacgctca ttctcgtcgt
     2581 gctttcgctg ctggtaaatg cgccacttct ggagcgtgca aacccgaacc agacgccgaa
     2641 cccgtccaag gcgccgtggt acttcctgaa tcttcaggaa atgctgctgc acatggacgc
     2701 cggattggcg ggtgtcatca tcccaacaat cgctctcggc ggtattgccg caatccccta
     2761 tcttgacaga agtccgctcg gtgtcggcat tctggggaca tcgagcaagg gtcgaaagat
     2821 catcgcgttc accgccgtgt acacgaccgt ggtgcttgta ggtatggtcg tgctcgacga
     2881 aatcacggaa cgcgggacgc agttcggcat tggcaaggcg ctccaggatc agggggcacc
     2941 cgagtgggtg ggcggcatcg tgctctcctc actctttatc atcgtttcaa ttgcagtact
     3001 cgtcggattg gtacaggcaa ttttcaaacc cacacgccgg gagctcatca tcgccctatt
     3061 caccggtttt ttcgtcacct actttgtgct cggcatcttc ggtacatcgt tccgtggcca
     3121 aggtcaaaac ctgacatggc cgtgggattt gccgcttgtt catcactagg cgttctcgcc
     3181 actatcccgg cttccggtgc gcatctgcca gttgcgggga caaggaatga tttccgtatg
     3241 aatccactcc tccgaacagt tgtcggtctg ggcacgggtt gggtggccag ccaaggtgta
     3301 gcagccgtgg cgcaccttca atccggtcac ggccagaccc tcgcaaagaa gcttcattcg
     3361 cgccgctcct ttacgcgcaa tgccgcgctt ggagcaacgg gtatcgtgtt ggcacaggcc
     3421 ggcgttgcat ttggcctgct gatgtggccg aacaaaacgg gcgcgttcgg cggcgaactg
     3481 accgtcggcg cagacagcat ccccgaagtg ggagcaacac cgttccggca tcaggagggc
     3541 aagttttaca ttgtccgcac cgaggatggg attgaggcgc tctactggaa atgcgtacac
     3601 ctgggctgca ccgtgccctg gatcgaggga gacgagctct tcatctgccc atgccacggt
     3661 tcggtctatg aatacaacgg ctcgcggatc gctggtcccg ccacccgtgc gatggacctc
     3721 atgccaacag aggtgggcga agacgggtcg gttacggtga acacaagccc ttctgcgctg
     3781 attcagcgtc cagagtactc cccggaccac gcgacgccgt atccattttc ctaggtggtg
     3841 ccgccaatat gaggtttgcc atcacagcga gtgacggcat gggatggata aggagtaaac
     3901 aagcgtggga gctgtacgaa agctcgcgac ggtcgtcatc atcggattgg tcgcgttatc
     3961 aaccctgatg gtggtgtacc tcgcggcaga gccggaccga cgcgggagcg aaacatcaga
     4021 acaacaatcc accatgttgg ttcgcggcac tgagctttac atcacctact gcttgcaatg
     4081 ccatggtcca gcgggtctgg gtgcggcagg tcgggatgga cgagttggag gtattctcaa
     4141 ccaagatgca gtcatccctg aggacgagca aggcgacctg aacgccaatt tccagtcaga
     4201 cgatccggtt gcccgctctg ttgccgagga ctggattcgt ttccggatcg cctatggcgt
     4261 cccgcccgac ccaattgcgg aagccaaagt catgccggcc ttcgcaaacg acctcaatgt
     4321 ggaggaaatg aacgctctgg tctatctcat catggacggc gattggaact acgtctacaa
     4381 caccatcgtg catgagactg gcgttcacgt agctgaggag gagtgcctgg agaacccgga
     4441 cgccgagtac tgtgacgacg tttcccaggc acctccggta tacccgacag cgcctccagc
     4501 cgggggtaat cccaacgcgg aaagcgacga ggcaaccacc ccggaatcag cagacgaagg
     4561 agctgcggac gatgccgctg cctctgaggg ggaatcagcg gcgggagcat cggaaggtgg
     4621 tgaggcagcg gccagcctcg aggcacagga tattgcctgg tctcagacgg aacttagggt
     4681 gaaacccggc gatacgatcg agatgacgaa caccggtcag ttgccgcacg actttacagt
     4741 tgatgaactg ggcatccagg aagtgacgcc aaccaatggc gacacagtga cgatcaccat
     4801 cccggaagat gccgaggacg gtgagtttga attctattgt tcggtcccgg gccacaagga
     4861 agcgggcatg gtcggcaccc tgatcatcga agccccgtag agacaggagc aggcagaaat
     4921 gctgatcaca aacctcgcca ccgcattcgg aaccctggcg gaaactgttg agctggcgct
     4981 ggcgtttatc atgatgattg gcggaattat catcactcac ctcatccagc ggtgggcaga
     5041 caagacaggc ggagagcaac cggcggcagc taacgtgggc caaggcggga tcgcccaata
     5101 cgaagacaac acaatgcgcg actagcctcg ttgtcttttt ggcagacatg accgaaagaa
     5161 cctgaccgcg gctgcggtca ggttctttgc ttccctcatc tcggcgaagg aaaagggcac
     5221 atgtcctcat cgtcctgacc accgactatg cgctgcgaca tagcaaaaag ccaatgacgc
     5281 gacatggagt tccacacggg gacatatcaa cggaacggaa cgaacgtggc aaatcgaaac
     5341 agcccgacga aaacatacct ctggtgcgat acatgtcgcc ggagctacag acacgaggaa
     5401 accaacagcg gtgcatgccc cgtttgcgga gccggaacgc gtgagctggg caagttctcg
     5461 gcgattctcc ggggcctcat ggccaacgag ctctccccct caccgctcgt ctcgaaacac
     5521 cgtcagctcg tgcgactgat ctggacgcac aacggcatgg gcgagcagta ctatcgggtg
     5581 atcgctcctg aaatgtcgta caaccggttc gaggcaaagg tgaccgaaat cctgtgccag
     5641 ggtgcagagg aaggatgggt agagttcgtg attccgcccg ctccacgcga ggaggaatcc
     5701 gcttaccagc tgaaacttgt agacgaagac cgatttatca gcgaacttca ggctctggcc
     5761 gggccggatg ccgcgatcga ggaatgaatg tcatgaggga tcacgaagag cgacttgaag
     5821 accgaacgat aggggactac ctcgacacat tggcatcgtc tgcgccagct ccaggcggcg
     5881 gcagtgttgc tggtctcatt ggtgctctct cggctgcact tggggaaatg gtcatcagcc
     5941 tgactcgtga cacggaaccg tcgctggagg aagcagcagg aaagctccga gcattccgga
     6001 gctcggcgat ggcgtcgagt gccgcggacg aaatggctta tagcagcttc gttgaagcat
     6061 caaggatgtc gaaggaaacc ggcgaggaca aggcgcgtcg cactgccacc atgcaggagg
     6121 cattggaaga agccgcagcg gtgccactgg aactcgcggg aacggcgatc cagttgctag
     6181 agcacttcga gccggttgtc cggttgggca gcaagcatgt cctctcagac gtggcaaccg
     6241 ctatcaccct tgctgtcgcg tgtgtcgatt gctccctcat caacgtccgg gtcaacctgc
     6301 ccatgatgaa gaacatcgag gtagccggca gtctacgtgg taaggcccag aaggtggagc
     6361 agcaagccca tgcgcttgcc gaggagctgc gcgacgaact caacaagaga taaagcatct
     6421 accgtccccc atggcccagc ccttggggac cggcccggtc gagtcggcgc gagcccgaag
     6481 tctcctcacc cgacccgcca gagcccgcgt ctagatttcg gcttgacgcg gccgatactg
     6541 gagagcctcc gtcacatgtt cggatcgaat ctggtctgat acggcgagat ccgcgatcgt
     6601 ccgcgccagc ttcagcacac gatggtacgc ccgagccgaa aggctgagct gtcgaaccgc
     6661 catgcgcacc aggtgctccc ctgcctcgtc cagccgcgca tgcttatgca cctctttcgg
     6721 ccccatatct gcatttgtga gcaactccga cccaacgtac cgctttgcct ggagctcacg
     6781 aggcgcttcg acgcgtcccc gcacagcggt cgatcgctct cccgaacgcc ggtccgccaa
     6841 ctgttcgtaa tcgatccggg gcacttccag atggatgtca atccggtcca gcaacggccc
     6901 actgatgcgc ttctggtaac gggtcactgc cgatggggcg cagcggcatt cccgcaccgg
     6961 atccccgaag aacccgcagg ggcaaggatt cattgccgcg atcaacgtca cgctggccgg
     7021 aaagctcagc gtgccagatg cgcgggccaa tgtcacccgg cgatcctcca acggctgtct
     7081 caatacctcc agcgtttgaa cgctgaattc gggaagctca tccaggaaaa gcactccccg
     7141 gtgggcgaga ctgatctccc cgggtctggg tcacgtgccg ccgccgacga gaccagcatg
     7201 gctcgtggtg tgatgaggcg accggaaggg tcggtcacgc actaaaggtg tctccggagg
     7261 cagtagtccg cggacgctgt agatacgcgt aacctcaagg gcctcgtcaa gactcattgg
     7321 gggcagaatc gaggggagtg cccgcgccag caatgtcttg ccggagccag gagggccggt
     7381 cattacgatg aatgagactt cacacctgca ccgttagttg ttgggattga tccgaggagg
     7441 gtccattctc tgccgttcgc gtcgatacgc tcgattaaag cttcaacggc gttccgcttg
     7501 atagtgaagt cggcggtgtc cagattgacg cctacccggt cgcagtacgt ccgtagcgca
     7561 aagctcgacg atagcgttct cctgctgatc gagccgtgca tcgatctcag cgagtgctgc
     7621 ctgagccgct cggcgttcgg cttctacccg ggtgatctct cgctcgacca actcccacgg
     7681 aaaaccgtca tcttcagctt cggcatagcg acgcacaagg cgctcccgct gcttctccat
     7741 cttgtgaagc atccgtgccg cggtttcacg actgttctgg agggtggcgt ctggaccatt
     7801 ctgtcggcgg cgttctactt cggcgatgat cgttgacggg tcgcgcagaa tggactcgat
     7861 ctgactccat gcccacattt cgacgtctac tcccggcccg cgcttaccgc cgcagggacc
     7921 gcccgcgcga tctcgggagc tacatcgaat aaacgtagga attgtgttct ggactggtgt
     7981 acatgggaca accacagacc gaacagaaga cgagaccgcg tagcaggtag ggccgagtcg
     8041 cattgcgtgt tgccgcgtgg gccatctgcg atggaacccg gacaccagca gacattgggc
     8101 ggatggtcgg tgcgggactc ggtgtcgagg tgcccgaggc ggtggtgcgc ctggccctga
     8161 gccagttggc cgaggtccat ttgctcgttg gcgtgctccc atccaaggtg caggagccgc
     8221 gtcagtcccg gcgggtgctg ctgcgacgga tggcgatcgc gggtggggtc gccctgccga
     8281 cactggcccc gatctccgcc ccgagcgccg tcgctgcgac ctcacactgt atacggcggg
     8341 ggcagggtga ctgcagacag accggtacgt cttgctgtac aggacttaca tgtcagccgg
     8401 atggtaaatg caccggcgga actgctgttt tataacgtct gccgtatgca ggtcgtctag
     8461 cagcctcacc acgcaacgca cgcacacgac gcggctacct gataccttcg ggggccgttg
     8521 tcgtgcgtgg tgggtgcacg atctctgatg ccagagccta ccggtcgacg agtcgggccc
     8581 ccagctgtcc gccaacggcc accaggatca tgaggatgag cccgatgagg agaccaccca
     8641 cggtcgcgaa cccggaggtc atcgcctctt cgctggtgtc tcgcaccaga ttgcccctct
     8701 ggatatccaa cgagacagcg agccagagca tgagcggcac gacgacaggc tggggtagag
     8761 cagccaggaa tgcccggttc gcggtcgtgg cggcgggcca gtgccagttc acgacgaatg
     8821 cgaccatcgg ggcgctgacc acaaacacga gcaaccagaa caaggcgagg gaacgcccag
     8881 gcccagggcc ggtcgtgccg aagagcggag ggatcaacat cacggcggtc gccagtgcgg
     8941 gcaagagggc agcaagcagg agtcgggtcc ggggatggac acgtgcgtga agagccactc
     9001 tgttccgcct ttccgtgcac cggtgagcca tctcgatcgt gagacattga tctccctgat
     9061 cctcctcgtc atacatctga cacgcatctg acacgcgtga gggctatcac cggggagcgg
     9121 cggagagaga gatga
//
LOCUS       DASYWM010000321         6315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_636607, whole genome shotgun sequence.
ACCESSION   DASYWM010000321 DASYWM010000000
VERSION     DASYWM010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636607"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VGR29_12700"
     CDS             <1..1024
                     /locus_tag="VGR29_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV2074482.1"
                     /translation="GTNCENLPETHEFLRKLRRMVDEEFPGKILLAEANQWPSDVVEY
                     FGSEEDPEFHMAFHFPLMPRMFMAMRQETRTPLVEIMESTPAISEHSQWAIFLRNHDE
                     LTLEMVTDAERDYMYFEYAKDPRMRINVGIRRRLAPLIDNGRRQIEMLNSLLFSMPGT
                     PVLYYGDEIGMGDNIYLGDRNGVRTPMQWSIDRNAGFSRADWARLYSPVIMDPVYGYL
                     AVNVEAQTRVATSLLNWMKRLIAVRKRYRAFGRGSIRFLHPDNQKILVYVRHYENETI
                     LCVVNLSRYVQAAEIDLSEYEGWQPVELMGETRFPHIGALPYFLTFGPHAFYWFRLES
                     PEQQPH"
     gene            1049..2467
                     /locus_tag="VGR29_12705"
     CDS             1049..2467
                     /locus_tag="VGR29_12705"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074483.1"
                     /translation="MSFEERAVEAVRQSESWIKNQRWYGDKGRSAHTLTPEVVVPVKI
                     ARLDAALVIARFAFDRGADSRYFVPLAATTGQIPTDGGVDLRDAMEDSDFLSWFIKGF
                     EDERLIEGEVTWRWRRLTHDFPSTHGLNFANARIISAEQSNTSVVFDNQFISKVFRRL
                     QPGINPDLEIGEFLSADGRFSHAPQLYGVVEMDSGEEPIAVAAIQQFVPNQGDGWNWL
                     LGQLSSLSVETRASLVDSVALLGTRTGEMHVALSSDTDDEAFSHEEFTRQDAEDLISR
                     VIAEIQESVEGLATRLSPPDVEQLHKGLGQLMGGAWSLVGAVKTRVHGDYHLGQTLRT
                     LDDDFCLIDFEGEPSRPMEQRRTKQSPLKDVAGMLRSLDYAVATVSGSTVDEAQRDAI
                     SAWHNDASTAFIEAYRSAIGGATTALVPEDEQRFTNGLNLLIAEKALYEVRYELNNRP
                     DWLPIPLNGIRRLAGIPIPQGT"
     gene            2484..3464
                     /locus_tag="VGR29_12710"
     CDS             2484..3464
                     /locus_tag="VGR29_12710"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV2074484.1"
                     /translation="MSNTAPQKLPANPGRLAPLMLAAIVLDVLLVVIMVTMIPLGFLR
                     GGLREVCSSAGLLLGILLALNWSDRWGRTISGGVALSEGTAQFLVAIALVVLCTAIIG
                     YGGSATFAYRPGPGGRMYGAFLALLNGIVFTGFLINQVTDDVYEGQLPNAVQEGYVSR
                     ALSVGFEWVLLVGALGVIAATVFGMFVRERSDDEFAFAPAPGPAMPEPLQATQRTAEV
                     PLDVPPAGDAEGETGGRPSSPVRIREVRHWEDQTPQRKGQPDYGGGWRQTWPEAKGKG
                     SALPWEQQPPSTERSSRPLPGPPGKGGQKSPSRRDILRDWMKEDSSRGES"
     gene            complement(3521..4312)
                     /locus_tag="VGR29_12715"
     CDS             complement(3521..4312)
                     /locus_tag="VGR29_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074485.1"
                     /translation="MERATGITRRALLTSVGLGILAGGTWGAAIQALATPSAPSLSVV
                     GKEDMQIVLLDTTEVRVLLLLGSPDDDLQSQIPGMLTMLRQRVDLLVGASSSVNALGS
                     SFRNRWQLVHTLAIAEPASPPVASETLTTVRDDLMIDVGNGVSLDLRIAVRGAWKQTV
                     NSHTLWAAIIRYGEHKIVLAPSGEALVELGATSPSLALVPVSPLGDIANTIGPGAIAT
                     NSRDDLTLQVQEPLGTVLVRIYAQDTARFELSRSGLTLPAWHERI"
     gene            complement(4328..5224)
                     /gene="ispE"
                     /locus_tag="VGR29_12720"
     CDS             complement(4328..5224)
                     /gene="ispE"
                     /locus_tag="VGR29_12720"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEV2074486.1"
                     /translation="MSTGSQARVLAPAKINLGLEITGRRADGYHDIRSVLAMVDLNDE
                     LTFTLRGNSRATRIDGMPHIPQADNLVTRAIVAFTSRTGIEAACDVGVQKRIPAPSGL
                     GGGSSDAAATLLVLNQSHGCPLREDEIHLLGASLGSDVPFFLGSPAALASGIGTTLAP
                     LPAPAGWVLIVVPSLDLRAKTAALYSSIRPMNFTDGRNVERIAECIRAGQSIPTELLT
                     NAFERPLEAIVPGIRDLVRRMSQAGCGNVALSGAGPAHYSIFETENLAHEVAQRMKGS
                     LKPGEQLFVAPFRTSPLTVKIQ"
     gene            complement(5221..6063)
                     /gene="rsmA"
                     /locus_tag="VGR29_12725"
     CDS             complement(5221..6063)
                     /gene="rsmA"
                     /locus_tag="VGR29_12725"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV2074487.1"
                     /translation="MTPTLQRSAANWQRTLRKHNIRPTRSMGQNFLIDPSIVSAIVEI
                     AEVGPEELVIEVGPGMGILTRELLSLGARVIGVELDRELAAFLREDLRDIGSFSLVEQ
                     DARHVVVEDITAGRPYRVVANLPYSAATVIIRRFMESPTPPSRMTVMVQKEVAERMTA
                     PQGEMSLLGLATDLYAQARIALIVPSEVFLPPPKVESAVIDLLVREALRGSQVTRDRM
                     FELATMAFQRKRKTVSNGLSMGLSRPKAELDQLLRSLDIDPKRRPQTLSVDEWLTIAG
                     ALPA"
     gene            complement(6060..>6315)
                     /locus_tag="VGR29_12730"
     CDS             complement(6060..>6315)
                     /locus_tag="VGR29_12730"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074488.1"
                     /translation="ASAVDGLLDIIAVADAGAFGIARALPRLLRSTHLSHPIVRHWKS
                     TGVTIESSTSILVETDGEQWDTMPHHYSLVPNALTWLEPI"
BASE COUNT         1247 a         1813 c         1970 g         1285 t
ORIGIN      
        1 gggaaccaac tgcgagaacc tcccggaaac gcacgagttc ctgcggaagc tgcgccgtat
       61 ggtcgatgag gagttccctg gaaagatttt gcttgcggag gcaaaccagt ggccttccga
      121 cgtggtggag tacttcggtt cagaggagga cccggagttc cacatggcct tccactttcc
      181 gctgatgcca cgcatgttca tggcgatgcg gcaggaaacc agaacgccgc tcgtcgagat
      241 catggaatca acaccggcta tttccgagca ttcccaatgg gccatcttcc tcaggaatca
      301 tgatgagctc accctcgaga tggtgaccga tgctgagcgg gattacatgt acttcgagta
      361 cgcgaaggat ccccggatgc ggatcaatgt cggcattcgt cggcgcctgg cgcctctcat
      421 cgacaatggc cgccggcaga tcgagatgct gaactcgctc ctgttctcga tgcccggtac
      481 gccggttctc tattacggcg atgaaatcgg catgggggac aacatttacc tcggtgaccg
      541 gaacggtgtg cggacgccga tgcagtggtc gatcgaccgc aatgccggtt ttagtcgggc
      601 ggactgggcg cgactctact caccagtcat catggacccg gtctatgggt atctggcggt
      661 caatgttgag gcccagaccc gtgtcgccac ctccctgctg aactggatga agcgactgat
      721 cgccgtgcgg aagcggtatc gggctttcgg ccgcggcagc attcgcttcc tgcatccgga
      781 caaccagaaa atactagtgt atgtgcggca ctatgaaaat gagacgatcc tctgcgtggt
      841 gaatttgagc cggtacgtgc aggccgcgga aatcgacctt tcggaatacg aagggtggca
      901 gccggtggag cttatgggcg aaacgcgctt cccgcacatt ggtgcgctac cgtacttcct
      961 gacgttcggg ccgcacgctt tctactggtt ccggctcgag agccccgagc aacagccaca
     1021 ctaacgctgg agggcaacgg tgatattcat gtcgtttgaa gagcgcgcag ttgaggcagt
     1081 cagacagtct gaatcgtgga tcaagaacca gcgatggtac ggcgacaagg ggcgttctgc
     1141 tcataccctc acaccagagg tcgtcgtccc cgtaaaaatt gcacggctcg atgctgccct
     1201 ggtgattgcg cgttttgcct tcgaccgtgg ggctgatagt cgctattttg tgccactggc
     1261 ggcaacaact ggacagatac cgactgacgg tggcgtggac ctccgtgatg cgatggagga
     1321 ttcggatttt ctgagttggt tcatcaaggg tttcgaggat gaacgcctca tcgagggtga
     1381 ggtcacctgg cgttggcgcc ggctgacgca cgattttccg tcgacccacg gccttaactt
     1441 tgccaatgcg cggatcatct cggcggagca aagcaacacc tctgtcgtct ttgacaacca
     1501 gttcatcagc aaggtgttcc ggcgcttgca accgggcatc aatcctgacc ttgaaatcgg
     1561 tgagttcctg tcggccgacg gtcgcttctc gcacgcgcca cagctgtacg gagtcgttga
     1621 gatggactcc ggcgaggagc cgatcgcggt agccgcaatt cagcagttcg tgccgaacca
     1681 gggtgatggc tggaactggc tccttggtca gctgagctcg ctgagtgtgg aaacaagggc
     1741 gtcgcttgtc gactcggtcg cgctcctggg gacgagaacg ggagagatgc atgttgcgct
     1801 ttcatctgat accgacgatg aggcgttctc ccacgaggag ttcacgcgtc aggacgctga
     1861 ggacttgatc tcgcgggtga tcgccgaaat ccaggaatca gttgagggcc tggcaacgcg
     1921 gctgagccct cctgacgtgg agcaactcca taagggactt ggacaactga tgggtggcgc
     1981 atggtccctc gtcggagccg tcaagacacg cgtacacggg gactaccatc tggggcagac
     2041 gttgaggacg ttggatgatg acttctgtct cattgatttc gaaggcgagc cttcgcgccc
     2101 gatggagcag cgacggacca agcaatcgcc gttgaaggac gtcgctggga tgttgcggtc
     2161 ccttgactat gcagtcgcga ccgtcagtgg atcgacggtc gacgaagcac agcgcgatgc
     2221 catatcggcg tggcacaacg atgcttccac ggcattcatc gaggcgtaca ggagtgcaat
     2281 cggtggggcg accacggcac tggtccccga ggatgagcag cggtttacca acggactcaa
     2341 tctgctcatt gctgagaaag cgctctacga ggttcgctac gaactcaaca accggcctga
     2401 ctggcttccg atcccgctca atggcattcg gcgactggcc ggcataccga ttccccaagg
     2461 gacatagcga gtcgtataca gtagtgagca acactgctcc gcagaagctg cccgctaacc
     2521 cgggaagatt ggctccgctc atgcttgccg ctatcgtgct cgatgtgctt ttggtcgtca
     2581 ttatggtgac catgatcccg ctcggattcc tgcgcggcgg tctgcgtgag gtctgcagca
     2641 gcgcgggttt attgctggga atcctgcttg ccctcaactg gtcggaccga tggggtcgca
     2701 cgatctccgg cggagtcgcg ttaagcgaag ggaccgcaca attcctggtc gcgattgcac
     2761 ttgtggtgct ctgcacagcg atcatcgggt acggcggaag cgcgactttc gcgtatcgac
     2821 cgggacctgg cggccggatg tacggcgcgt tcctggcgct gctcaacggc atcgttttca
     2881 ctggtttcct gatcaaccag gtgaccgacg acgtttacga aggccagctt ccgaacgccg
     2941 tgcaggaggg ctacgtctca cgggccctct ctgttggctt cgagtgggta ttgctggtcg
     3001 gtgctctcgg tgtgattgcg gcgaccgtgt tcggcatgtt cgttcgagag cgatctgacg
     3061 atgaattcgc tttcgccccg gcgccgggac cggccatgcc ggagcccctg caggcgacac
     3121 agcgaacagc ggaggtgcct ctcgatgtgc cgccagccgg agatgcggag ggggagacag
     3181 gtggacggcc atcgtcaccc gtccgtatcc gtgaggtgcg ccattgggag gaccagaccc
     3241 ctcagcgaaa agggcaaccg gattatggcg gtggttggcg ccagacgtgg ccggaggcaa
     3301 agggaaaggg atcggctctc ccttgggagc agcagccacc atcaactgaa cgctcgtcca
     3361 gacctctgcc tggccctcca ggcaagggag gacaaaaatc tccatcgcga cgggatatct
     3421 tgcgcgactg gatgaaggag gactcgtcgc ggggcgaaag ctagagcgtg catcctgtac
     3481 aacggctggc gtccaccgtg gatatgtacg cggagagcgg tcagatacgt tcatgccagg
     3541 ccggcaacgt aaggccgctt ctgctgagtt cgaatcgtgc agtgtcctgc gcatagatac
     3601 ggacgagcac cgtgcccaac ggttcctgta cctgaagggt gaggtcgtca cggctgttcg
     3661 tggcaatggc acccgggccg atggtgttcg ctatatctcc gaggggcgat accggtacca
     3721 gagccagtga cggcgaggtc gcgcccagtt ccacgagcgc ctctccgctt ggagccagaa
     3781 caattttgtg ctctccatag cggatgatgg ccgcccatag cgtatgtgag ttcaccgtct
     3841 gcttccaggc gcctctcaca gcaatacgga gatccaggga cacgccgttc cctacatcga
     3901 tcatgaggtc gtctcttacc gtcgtgagtg tttcactcgc gacgggaggg gatgcaggct
     3961 ccgcgatggc aagcgtgtga acaagttgcc agcggtttcg gaagcttgat ccaagtgcgt
     4021 tcacggagga ggaagcgccg acaagcaggt caactcgctg gcgcagcatt gtcaacatcc
     4081 ccggaatctg actctgaaga tcatcgtccg gtgaaccaag gagaaggagt acacggactt
     4141 ccgtggtgtc cagcaggacg atctgcatgt cctccttgcc gactaccgac aaggatgggg
     4201 cagaaggcgt ggcaagtgcc tggatggcgg cgccccatgt acctcctgcc agaatgccaa
     4261 gtccgacgct ggtgagcagc gctcttcgtg tgattcctgt cgcgcgctcc atgatccgat
     4321 ccggatgcta ctggattttg acggtcaacg gtgatgtgcg gaaaggagca acaaacagct
     4381 gttcgcctgg cttcagcgat cccttcatcc gctgtgcgac ctcatgcgcc aagttttccg
     4441 tctcgaatat ggagtaatgt gcaggtcccg cgccgctaag tgccacgttt ccacatccag
     4501 cctgactcat gcgcctgacg agatccctga tacctggcac gatggcttca agcggacgct
     4561 cgaatgcgtt cgtcagaagc tctgtcggga tgctctggcc ggcccgtata cactctgcga
     4621 tccgctcgac gttcctgccg tcggtgaagt tcatcggcct gatgctgcta tagagggcgg
     4681 cggtctttgc tcgcaaatcc agcgatggga caacgatcag cacccatcct gcaggagcgg
     4741 ggaggggggc aagggtcgtc ccaattccgc ttgccagggc ggcaggagat ccgagaaaga
     4801 atggcacatc gctgccaaga cttgcaccga gcaggtggat ctcgtcttca cgaagggggc
     4861 acccatgcga ctggttgagg accaggagcg ttgccgcggc atcggaactt ccgccaccga
     4921 gtcccgaggg agcaggaatt ctcttctgta cgccaacgtc gcaagcggcc tcaatgccgg
     4981 tacgtgaggt gaaggcgacg atcgcacggg tgacaagatt gtccgcctgc ggtatatgcg
     5041 gcatgccgtc aatgcgggtg gcgcgtgaat ttccgcggag cgtgaacgtg agttcgtcgt
     5101 tgagatcgac catggcaagg accgatcgga tatcgtgata gccatccgcc cggcgtcccg
     5161 tgatctccag gccgagattg atcttggcgg gggctagcac gcgcgcctgt gaacccgtgc
     5221 tcatgctggg agcgcacctg cgatggtgag ccactcgtct acggagagcg tttggggccg
     5281 tcgtttcggg tcgatgtcga gcgacctcag gagttgatcc agttccgcct ttggccggct
     5341 cagacccatc gaaaggccgt tactgaccgt tttgcgcttg cgctgaaacg ccattgtcgc
     5401 gagctcgaac atccggtctc gtgtcacctg gctgccccgc agcgcctcgc gaaccagaag
     5461 atcgattacg gcggattcga ccttcggcgg cggcaggaag acctcggagg gaacaatgag
     5521 cgctattctg gcttgcgcat acaggtcagt cgcaaggccg agcagggaca tctctccctg
     5581 tggtgccgtc attcgctcgg caacttcctt ctgcaccatg acggtcatac ggctcggggg
     5641 tgtaggcgac tccataaacc gccggataat gactgtggcg gcgctgtagg gcagattcgc
     5701 aacaacacgg taggggcgac ccgccgtaat atcctcgacc accacgtgtc gagcgtcttg
     5761 ctccaccagt gagaagctcc cgatgtcccg gagatcctct cgcaggaatg ccgccagctc
     5821 gcgatccagc tcgacaccga tcactcttgc accgagagaa agcaactcgc gtgtgaggat
     5881 gcccatgcca gggccgacct cgatcaccaa ctcctcgggc ccaacctcgg caatctcaac
     5941 gatcgccgac acgatcgatg gatcgatcag aaagttctgt cccatggagc gagtcggccg
     6001 aatattgtgc ttgcgcaggg tgcgctgcca gttcgcagcg gatcgctgca gcgtcggtgt
     6061 catatgggct ccagccatgt cagcgcattt ggtaccaacg aatagtggtg cggcatagtg
     6121 tcccactgct cgccatccgt ctcgacgagg atgcttgtcg atgattcgat cgtcacaccg
     6181 gtgctcttcc aatgccgcac gatcgggtga ctgagatggg tggatctcag gagccgcggc
     6241 agggcacgcg cgataccaaa cgctccagcg tccgctacag cgataatatc gaggagtccg
     6301 tctacagcgc tggcg
//
LOCUS       DASYWM010000322         3914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_205122, whole genome shotgun sequence.
ACCESSION   DASYWM010000322 DASYWM010000000
VERSION     DASYWM010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205122"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VGR29_12735"
     CDS             <1..423
                     /locus_tag="VGR29_12735"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV2074489.1"
                     /translation="EVICHNDFAPYNMVFEQQRITGVIDFDMASPGPRLWDLAYLAYR
                     LIPLTGGTNPDGVRSDEKTRLSRLDTLIAAYGSPFSRLAFLGMVQQRLRHLARFSEEL
                     ADETGRVQLQEHAALYRSDSEYIQQLLAGLTAEPGTTA"
     gene            521..724
                     /locus_tag="VGR29_12740"
     CDS             521..724
                     /locus_tag="VGR29_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074490.1"
                     /translation="MDKILPKDQGKHDILIVVLIAVPLMVAGFVWAMIDGGYTDPAEP
                     GNGISIPAPSPAASPAFLATPES"
     gene            1055..2542
                     /locus_tag="VGR29_12745"
                     /pseudo
     CDS             1055..2542
                     /locus_tag="VGR29_12745"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
     gene            2539..2907
                     /locus_tag="VGR29_12750"
     CDS             2539..2907
                     /locus_tag="VGR29_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2074491.1"
                     /translation="MKRVLVVDDEPFIRQVIGDVLQDEGYDALFASSGHAMLKLLETE
                     HPDLVLLDLMMPDGDGQEALQAMQSQPHLADIPVVVVSTGVARQRLDGVTVRFLAKPF
                     DLDKLLQIVIDTIGPALAPE"
     gene            complement(3038..>3914)
                     /locus_tag="VGR29_12755"
     CDS             complement(3038..>3914)
                     /locus_tag="VGR29_12755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV2074492.1"
                     /translation="AMRVEDLLVDGQDWTDEEYIRYLSEGPWLGEIELRRKGGKRVPV
                     EVLATVVELPTGPVYLSALRDVSERHRLERLRRDFLAMVTHDLRTPLTTVRGNAQLLK
                     RRAEYRPAQVDAVLAATAQMQQLINNLADVVVLEGGSVALRKVPVDLTVLVDEVAKEL
                     QDAFEARRVTIDTPATAVFGAWDRARLKQVLTNLMENALKYAAPDSDVVVQVEDCGDE
                     ARVTVKDQGLGIAPEHLPRLFDRFYRADVTGVGGLGLGLYISRMLLEAHGGRIWAESE
                     VGQGSTFTLALPKHA"
BASE COUNT          757 a         1249 c         1132 g          776 t
ORIGIN      
        1 gaggtgatct gccataacga tttcgctccg tacaatatgg tgttcgagca gcagcggatc
       61 acgggcgtga tcgattttga tatggcctct cccggaccac gtctctggga tctcgcttac
      121 ctcgcctacc gtctcatccc cctcacgggc gggacaaatc ccgacggggt tcgctctgat
      181 gagaagacgc gactatcccg tctcgatacc ttgatcgcgg cctatggcag cccgttctct
      241 cggttggcct ttcttgggat ggtgcaacag cggctccggc acctggcgcg tttcagcgag
      301 gagcttgccg atgaaacggg tcgggtgcaa cttcaggaac atgcagcgct ctatcgttcc
      361 gacagtgagt acatccagca actgctggcc gggctcactg cggaaccggg caccaccgcc
      421 tgatccggga gagtgcggca agcgtgtgtg cgatagttga tggatattgc acgccgcggc
      481 cagtgcggat cacgctacgt tcccggagcc cagttaacac atggacaaga ttctgccaaa
      541 ggatcagggc aagcacgata tcctgatcgt cgtcctcatc gcggttcccc tgatggtggc
      601 tggcttcgtc tgggcgatga ttgacggcgg ctacactgat ccggctgagc cagggaacgg
      661 tatttcgatt ccggctccct cccctgctgc aagcccggcc ttcctcgcca ccccggaaag
      721 ctagaggttt ttccaggccc atccgggaaa ggttcaaaca tcctctgaac ctccaattgg
      781 gtcgtcgcct tgctctcatc aagcgtgcag ctcctagaga aaatctcctt tgccggcggc
      841 agatgcaaca cggtcatcac tagaggtccg ttcacttggc accacatcag ctggtcaacc
      901 aaacacccct ggaaggaccc atttcggggt ggcgacacgt gctccggccg ttgtgtcaag
      961 atgatttgca ccggattccg ggcatgtcat gtgcgcacgc cttggcgaga agcaattgat
     1021 ctgcgtttca tatccagttg gatgaaagga caagatgagc gagcagcggc agtcaggtga
     1081 tggaccaacc ctggagcggt taaggaccgg aatcgcgggc tttgacgcca ttactgatgg
     1141 aggactgccg actcgacgta cgttcctggt tacgggggcg cccgacactg gcaagaccac
     1201 cttcggcaac cacctggcgt ttgcccacgc cgcccggggc ggccaggtga tcgttgccac
     1261 cttgctaacg gaatcgcacg atgtcctcct gcaggacctg cagggttttc gcttctttga
     1321 tgcggacatg gtgggccacc gggttcacta cctgagcctc ctgaccccgc tcatggaagg
     1381 cgggctgaat gccaccatcg atgtcctgcg ccaggaagta cggcaggcca acgcgacgct
     1441 gctggtcgtt gacggcacgg cgatcgcaga tgatttcgcc tcgtcgagct tcgacctccg
     1501 ccgctttgtg caacgacttg aatcccaggc ttcactcctt ggctgcacca ccgtgcttct
     1561 taccagcacg ggtgccagga actccgaccc actgtgggcg catgccaacg gtgtggtctt
     1621 gctgacaaac catctcgtca gttcccgtca tgtgcgcacg ctggagctgg tcaaattgcg
     1681 cggtgccggg tacgcaaatg gagcacatga gttcgcggtc agggaggacg gaatcaccgt
     1741 cttcccccgc ctggagtcct tggcaggacg ccatcggcgg gcccaaagga cgcagcatgg
     1801 gctggggact ggtattcccg gactggactc gatgctgggg ggagggctca tgcccttgtc
     1861 aaccaccatc gtcatgggga cgccgggagc cggcaagacc atcctcggat tgcacttcct
     1921 gacggagggg gcaattcggg gagaacgcag cctgttcgcc ggcttccacg agacggagga
     1981 cgatctgtgt accacggctg aagggatcgg gctcgaccta agcaaccaca ttgacagtgg
     2041 gctgatccgg gtcctctgaa gcccgcccct ggagctctcg gtcgacgatt gggcttggcg
     2101 ggtcttgacc gcaatcgaag agcacaagcc cacccgcgtc ttcattgatg cgatcacgga
     2161 tgtgtatcgc ctcataaccg agcctcagcg gacgccaatg tttgtctctg cgttggtcaa
     2221 tgagctacgc actcgtggtg caacggccct gatcgcggcg gagatcgacg aatacacaag
     2281 tgagaagctg gcagtaccaa tccccgctgc atcagctgcg atggataacg gcatcttgct
     2341 acggcacccc gaagtccagg gagagctacg acgcctggtt gcggtgccga aggtgcgcca
     2401 gtcggcatct gatccatcca ttcgcgaatt ccagatcacg gaccgtggta tggtcgtcac
     2461 tcgccccttc tcggcaacct ctggccttct cactggccgc ggcgaaccca caagtaagga
     2521 gggcggggac gagccgtcat gaagcgagtg ttagtggtag atgatgagcc gttcatccgg
     2581 caggtcatcg gcgatgtcct ccaggacgag gggtacgacg ctctcttcgc cagcagtggc
     2641 cacgcgatgc tgaaactgct cgaaacggag catcctgacc tggtccttct tgacctgatg
     2701 atgcccgacg gcgatggaca ggaagcgctg caggccatgc agtcgcaacc gcacctggca
     2761 gatattcccg tcgtggtcgt gagcacgggc gtcgcccgtc aacggctgga cggggtgact
     2821 gtaaggttcc ttgccaaacc ctttgatctt gacaaactgc tgcagatcgt gatcgacact
     2881 atcggccccg ccttggcgcc tgagtagtcc tagccagcca ccacaaacgc ctcctgaccg
     2941 gccccgggtc ggatggtcgg gatacatgcc gatatcgggc accgcggcgt tgacgggctg
     3001 gtaacttcct tacctgcttc ccataaagga ctgccgctca ggcgtgcttc ggcagcgcca
     3061 gcgtaaaggt actgccctgc cctacctctg attcggccca gattcgaccg ccatgagctt
     3121 cgagcaacat ccggctgatg tagagcccca accccaagcc ccccaccccc gtgacatccg
     3181 cccggtagaa ccggtcaaag agtcgaggca gatgttcagg ggcaatccct aggccctgat
     3241 ccttcaccgt cacccgtgcc tcgtcaccgc agtcttcgac ctgaaccacg acatcggaat
     3301 ccggtgccgc gtacttcaaa gcgttctcca tgaggttggt caatacctgc ttcaaccgcg
     3361 ccctgtccca cgctccaaac accgcggttg caggggtgtc aatggtcact cggcgcgcct
     3421 caaacgcatc ctgcagttcc ttcgccacct cgtccacgag aaccgtgagg tcgacaggca
     3481 ccttccgcag ggctacagag cccccttcca gcaccacgac atcggccagg ttgttgatca
     3541 gttgctgcat ctgggcagtg gccgcgagca cggcatccac ctgcgctggc cggtactcgg
     3601 cccgtcgctt caacaactgc gcattacccc ttacggtcgt caagggagtg cggagatcgt
     3661 gggtcaccat cgcaagaaaa tctcgtcgca gccgctccag ccgatgacgc tcggagacat
     3721 cacgcagtgc cgacagatac acgggcccgg tcggcagctc cactaccgtc gcaaggacct
     3781 cgaccggcac tctcttccca cctttgcggc gtagctctat ctcgcccagc cagggacctt
     3841 cggacagata ccggatgtac tcttcgtccg tccagtcctg cccgtccacc agcaaatcct
     3901 ccacccgcat cgcc
//
LOCUS       DASYWM010000323         4345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_468477, whole genome shotgun sequence.
ACCESSION   DASYWM010000323 DASYWM010000000
VERSION     DASYWM010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468477"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..750)
                     /locus_tag="VGR29_12760"
     CDS             complement(55..750)
                     /locus_tag="VGR29_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074493.1"
                     /translation="MVDDFLEAIQRPISSPRLHSYGTPSDELATKLLNYFWNIELSQA
                     FYPLLQAYEVALHNTIHHTLSDHFETEWWFDLPQVLGQDQLETVAEAKADLERKNKIL
                     TSGRLVAELMFGFWTSLLNRPFEEPLWHTGRPILLLSAFPNLPKHRRTLRAVYDRTQL
                     ANTLRNRIFHYEPIWNRPTLQADHDHILEALGWISTEIHEAVTFYDRFNDVYGRGSDR
                     SRQALWHHLDLEP"
     gene            1186..1329
                     /locus_tag="VGR29_12765"
     CDS             1186..1329
                     /locus_tag="VGR29_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074494.1"
                     /translation="MTNWGEPKETCWIVTVMIQRDFYHAGEINHIRALHNRADRWAYD
                     RDR"
     gene            complement(1560..1919)
                     /locus_tag="VGR29_12770"
     CDS             complement(1560..1919)
                     /locus_tag="VGR29_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074495.1"
                     /translation="MRAITLAALAAGYVDPGEFLTMPNTVYVCPVACHREFGERLLVR
                     DPDGAWFLWMGDGGMPELIRVPARLAGWIADHPHMVPVASPGEVPHMWFEVSSLPVTP
                     VAPVAPVPTERAGPPPF"
     gene            complement(2470..2883)
                     /locus_tag="VGR29_12775"
     CDS             complement(2470..2883)
                     /locus_tag="VGR29_12775"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV2074496.1"
                     /translation="MATSHLSPAPADPALTQSESHKAAVPAAAPPAEHPDGVTERRPH
                     PDAYGEDKTKILKRLRRIEGQVRGISRMVEEDKYCIDILTQISAIVASTRSVGLLLLE
                     DHIRGCVVNAGPDEREATLEELNRAIERFTRSVGS"
     gene            3365..3547
                     /locus_tag="VGR29_12780"
     CDS             3365..3547
                     /locus_tag="VGR29_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074497.1"
                     /translation="MRSIIIRGMLAGVAGMAAMTAGEMLEQMVTHRPNSFVPAHTLER
                     LLGLPRKRDSERLWLN"
     gene            3551..3808
                     /locus_tag="VGR29_12785"
     CDS             3551..3808
                     /locus_tag="VGR29_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074498.1"
                     /translation="MHWGQGILLGAARAFMAERGVRGPVGSFLFWNLRLLTDQTLENA
                     TGAGALPWTWPTDEQVIDITHKGVYAFTTGIVVDRLVAARH"
     gene            complement(4037..>4345)
                     /locus_tag="VGR29_12790"
     CDS             complement(4037..>4345)
                     /locus_tag="VGR29_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEV2074499.1"
                     /translation="GQATPIAQNMTSVLVKSAREVASLVGFIGGGTGYRPYVARLHEY
                     EHEGDDLWMEGYAALFSGDLAPLDVIRWKDIYEQLEAAIDGCEAVARVIELSLDRIRT
                     "
BASE COUNT          840 a         1287 c         1398 g          820 t
ORIGIN      
        1 gctgtgtggc tctcgcgcgc tcgtgtctag gtatttgatt ggcagcgaca gcagtcacgg
       61 ctccagatcg agatgatgcc aaagcgcttg ccggctgcga tcactccctc gtccatagac
      121 gtcattgaac cggtcgtaga aggttacggc ctcgtggatc tcggtgctga tccagcccag
      181 agcttcgaga atgtgatcgt ggtcagcctg gagtgtgggc cggttccaga tcggttcgta
      241 gtggaagatg cggttgcgta gcgtgttggc aagttgggta cggtcgtaaa ccgcacgaag
      301 tgttcgtcgg tgcttgggaa gattggggaa ggcgctcaga agcaggatcg gccggccggt
      361 gtgccagagg ggctcttcaa agggacgatt caagaggctt gtccagaagc caaacatcag
      421 ctctgcgacg agccggcctg aggtcagtat cttgttctta cgctccaaat cagctttcgc
      481 ctcggccacc gtctccagct gatcctgtcc cagcacctgg ggaaggtcga accaccactc
      541 cgtttcgaag tgatcactca aggtgtggtg gatcgtattg tgcagcgcaa cctcgtaggc
      601 ctggaggagt ggatagaacg cctgggacaa ctcaatattc cagaagtagt tgagcagctt
      661 ggtcgccagt tcgtcgctgg gagtgccgta ggagtgcagc cgtggtgagg agatggggcg
      721 ctggatggct tccaggaagt catcgaccac gggcaaatcc tgagcgaggg gtgacagaac
      781 cccgggacca tcaccggctc taaggcacgg gagaggatac ccggggaatg acacctgcaa
      841 ttctagttgc gtagtttagg caagtcaatg cataagatgt accccacagt atctgagtgt
      901 cgctgtatgg gatggcgttc tttcataggt tgggtgcgcg gcggtcgacc tgcgcatcca
      961 tggtcaggat ctctctgatg aaaggcccgg ggccgatctg aatctaagct cctgaacttg
     1021 tccccgtaag gatcccgact ccctccacgg tactgatgat cctgccagcc accctgcgga
     1081 tctcttcaac gttggtctga aggcaatccc tagctgtgcg acgggcacga ccgcctccgc
     1141 cgctccttcg ccacactcac cgacgatgcg ctcgatcggc cccggatgac gaactggggc
     1201 gagccgaaag aaacgtgctg gatcgtgacg gtgatgatcc agcgcgattt ctatcacgcc
     1261 ggggagatca accatatacg ggcgctgcac aaccgcgcgg accgctgggc gtacgacagg
     1321 gacaggtgac ggacgacggc ccaaagattc tcaggttcca cgctgctatg cgacgccccc
     1381 gatccttggg agaggtgtgg cgggaacgca ttgtgaagtg cataagaagg agcggatgtc
     1441 acgggcggct ccggacccga ccacagggaa ccgcgtaggt gctgacatgc tgacccgctg
     1501 ccatgacgat cggatgtgca gcgtagcgct tcgccccaag gcgcaccgca ccgatgcgat
     1561 cagaacggcg gcggccctgc acgctccgtg ggaaccggcg caactggtgc aacgggcgtt
     1621 accggcagcg acgagacctc gaaccacata tgcgggacct cgcccggaga cgcgacaggg
     1681 accatatgcg gatgatcggc gatccacccg gcaagacgcg cgggtactct gatcagttcc
     1741 ggcatcccgc catcgcccat ccagaggaac caggcaccgt ccggatccct gaccaggagg
     1801 cgctcgccaa actcccggtg acatgccacc ggacagacat agaccgtgtt cggcatcgtc
     1861 aagaactctc ccggatcaac gtatccagcc gcgagcgccg caagggtaat cgcgcgcatc
     1921 gcgcatgctg aggggctgct gcgatcgggc ccctgaccac gcctgagccc gtttgccagg
     1981 cggcaggaac aggccgagca tctggaccgg aattcagcac atcaggaaaa gggccgcggc
     2041 gggtgatgtt acgaccgccg gagaattgca cggcgtggtg ccgtaccaga acgggtggat
     2101 cgaatgcgga gggaccttgc ggcactccgg gcccgagtca ttgcggtgaa cgcaatgcgc
     2161 ttgccatgtc accgacagaa ccgaacgagg gatcgcccag gttatcgact gcgccagctg
     2221 agccggcagg tcaggggatg gtcgcacttt gagatttgtt gtgatccccg tgcgttttga
     2281 atctggatgc ggtcgagcgt aaaacatctt gccgcgggga attacgagaa tggcccctgc
     2341 gactggatgt cgacggtcaa ctgggtagga tggctgcgcc accgcctatg cgcgggagct
     2401 accggtcgac tggcgtctaa tcgtccattg ggatcgccgc catgacatgg acgctggcgg
     2461 tgggccggtt cagctcccga cgctccgggt gaagcgctcg attgcccggt tgagctcttc
     2521 gagcgttgct tcccgctcat cgggtccggc gttgacgaca cagccgcgga tgtgatcctc
     2581 cagcagcaga aggcccaccg agcgggtgga ggcgacgatc gcggagatct gggtgaggat
     2641 gtcgatacag tacttgtcct cctcgaccat gcgggagatg ccccgtacct gaccttcgat
     2701 ccggcgcaac cgcttgagga tcttggtctt gtcctccccg taggcatcgg gatgcggacg
     2761 gcgttccgtc accccgtcag ggtgctccgc tggcggtgcc gctgccggta ccgctgcttt
     2821 gtgggattcg gattgggtca gggccggatc ggcgggtgcc gggctgagat ggctggtcgc
     2881 catggtgatg gtctcctgga tggtggcgtc gccggagcaa gggtgagatg cacgctttgg
     2941 cagccccaac cacgacgccg ttggtctgtc tctagtatgc cactactcca tggggaaccc
     3001 ggcagcaccg acgatccgcc gtcccgaaat cgacgttcca gtttggccga tccgatcagc
     3061 agaggaaacg ttccgggcat gtgcggaccc ctgtcttgcc gatgtcatgc gcaggccgca
     3121 tccactggtc accacccgtg acccctggag gagcgggacg cgctccgcct gacgacgatt
     3181 cccgcggtca aggacctgac ggaccaacca tacccgttcc tcttacctga gcatgccggg
     3241 cgatgtcacg ggctccatcg gtcaacgtag gcacggcact accatgcgca gcaggattct
     3301 cacatggcgg cggagctcca ctgatgcctc gccaggacaa gacaggacaa gaaggatatg
     3361 aatcatgcga tcgatcatca tccgtgggat gctcgccggc gtggcgggta tggcggccat
     3421 gacggctggc gagatgctgg agcagatggt cacccatcgt ccgaactcct ttgtcccggc
     3481 acatactctc gaacgattac tgggtttgcc gcgcaaacgc gacagcgaac gactctggtt
     3541 gaactgaacg atgcactggg ggcaaggcat cctgctgggg gcggcgagag cgttcatggc
     3601 tgaacggggg gtgaggggtc cggtcggatc gttcctgttc tggaacctgc ggctgttgac
     3661 cgatcaaacg ctggagaacg caactggtgc cggcgcgctg ccctggacct ggccaaccga
     3721 tgagcaagtg atcgatatca cccacaaggg tgtctacgca ttcacgaccg ggatcgtcgt
     3781 cgatcggctt gtggccgcac gtcactagcc gaccatcccg gtccttccgc tcaatggacc
     3841 ccgatggaac gcctgcgatg cttgggttcc catccccgac cgtgcatgtc ttatcgccgg
     3901 aaacgggcaa ggaagtcagg ggtgaaccgc ctgtcaatgt gatgcatctg cggaccgaaa
     3961 cacggaccat ccctcccgca tgtcctatct gatgcgcgcc cgattcatcc cgcgggaggt
     4021 gaagttgtct cgttcgttat gtccggatgc ggtcgaggga gagttcgatc accctggcga
     4081 ccgcctcgca gccatcgatc gccgcttcaa gttgttcgta gatgtccttc cagcggatga
     4141 catcgagcgg cgcgagatcg ccggagaaga gcgcggcgta cccctccatc cacaggtcgt
     4201 caccttcgtg ctcgtactcg tgcaggcggg cgacataggg acgatagccg gtcccgcccc
     4261 cgataaagcc gacgagcgag gcgacttccc gggcggattt gacgagcacc gatgtcatgt
     4321 tctgagcgat cggggtcgcc tggcc
//
LOCUS       DASYWM010000324        19124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_280981, whole genome shotgun sequence.
ACCESSION   DASYWM010000324 DASYWM010000000
VERSION     DASYWM010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 19124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19124
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280981"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..782
                     /locus_tag="VGR29_12795"
     CDS             102..782
                     /locus_tag="VGR29_12795"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEV2074500.1"
                     /translation="MTTTRDKMLNSLLTSTMNRRRMLINAGIVGGSVAAMGTLGSVGA
                     QTNATPAADAGAGVAFQNPVEVLNFALTLEHLEAAFYRDGLAQFGVEGITALGFQQSV
                     FDSLNEIAQHEVTHVDTLTQVITDLGGQPVAEAQYDFGYADAAGFLTTAQALEDTGVS
                     AYQGAASSLIESDELLTAALTIHAVEARHAAYLALLNAMIPFPEAFNPTLTPNQVLEI
                     AGPFIVSQ"
     gene            complement(1006..2709)
                     /locus_tag="VGR29_12800"
     CDS             complement(1006..2709)
                     /locus_tag="VGR29_12800"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEV2074501.1"
                     /translation="MAVSRATGLQVGRSRWLTIIVVLGAFLVGGLLTPSQVSAHAELV
                     NTTPAEGALLETQPDQVTLTFTEAVEPVQEAFRLFNSDGLVATLETPATGETLEIALP
                     GDLADGTYVVDWRVVSLDSHPITGALTFSIGEESGAPVEVATSSSDVTPIASIVQGIA
                     YLSLLSTVGLIWFRVFVVRDVDGRTISLATGGAILSAIAHLLLIPLTTIRQVGESIGA
                     LADPEMWEVEPLSVTLRAVIFVVAGLAIEIHFARSSDATRRTRRAVALGGLLALGSLT
                     VVGHTADIPPTWLMHGSDFVHGAAGAIWFGGLLGLGRYLYVALRTRSSGNRPGPGDAA
                     QVVARFSWLAGWAFAALAISGAVIAFKILGSWDALLHTTYGRILIAKLSLVVIPFGLA
                     VWNRLRLVPSVQRDPEASSAWNRLRTTVLTEAGLLVVVLSLTGFLVLQNPAVAGSDGV
                     EAVAAVPFEEAEALGDGSLEITITPAMTGENEISLMVHDADGLPIQLLVDPEIRLSLP
                     EAGLGPITSTATSAGHPGHYASQVQVPFEGEWTVEVVTRISKYEEPMTTFTVPIPHLP
                     D"
     gene            complement(2950..3510)
                     /locus_tag="VGR29_12805"
     CDS             complement(2950..3510)
                     /locus_tag="VGR29_12805"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HEV2074502.1"
                     /translation="MRRVRQLALGVGGAALLAVAGVAPVSAHVGITADETAAGAHTLL
                     TVGFSHGCEGSPTTKISISVPEEITSIAPGMNYGWTVEKVMESEATPAATPAEGAHGE
                     GGRVSEIIYTAKEPLPDGFYDQFLLSVQLPEDGEGETLYFPIIQTCEEDETAWVQIPE
                     EGQDDDELEAPAPGVTIAAPEEESEE"
     gene            complement(3694..4038)
                     /locus_tag="VGR29_12810"
     CDS             complement(3694..4038)
                     /locus_tag="VGR29_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEV2074503.1"
                     /translation="MLLKGYLWLLLRNRNRSNHGCGTLHARFVEREKEPSTRTSFCWA
                     SCSTNGRLNFNDELTGMDRELGDYVIVHELLHFSAPNHGRLWKMLMRVHLGDYERLEA
                     RLRTNPATELKP"
     gene            complement(4348..4770)
                     /locus_tag="VGR29_12815"
     CDS             complement(4348..4770)
                     /locus_tag="VGR29_12815"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEV2074504.1"
                     /translation="MNSPDRVSYFYSVLRLVPRVERGECFNVGVAMLSRSRRFLAVRT
                     DLDEEKLAVLAPGVHPEIIREQLVALETVAAGDPAAGPMAALEPAERFHWITSPSSTI
                     IQPSPVHSGLTTDPQLTLDRLFNELVGPVERALDDQMK"
     gene            complement(4763..5620)
                     /locus_tag="VGR29_12820"
     CDS             complement(4763..5620)
                     /locus_tag="VGR29_12820"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEV2074505.1"
                     /translation="MTDLPLVTPEVREVTATRFVLPLREGGSVPGLFEADDDGLYVVK
                     FHGAAQGPKTLTAELLAGEIGRKLGLRVPELVLVDLPVELSIGEPDLEIQDHLARSVG
                     LNLGLDFLPGALPFDPATRTPVDPVLAADIVWFDAYIANVDRTTRNPNMLRWHDGLWL
                     IDHGAAIYPHHRWQDPAAQGRRSFAAIKDHVLLPVAGSLVDADARMAARLDESVLWSI
                     VGSIPEAWLPENQEIGNAEQQREAYMAYFTARLEAPRPFIEEGEHSRTSEGVGAIDPH
                     RATRGRRRE"
     gene            complement(5795..6253)
                     /locus_tag="VGR29_12825"
     CDS             complement(5795..6253)
                     /locus_tag="VGR29_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074506.1"
                     /translation="MQTQSQDQDTTTTAPFGVNSGPPASQTPSSRPVAASDILERIDM
                     LIAETAYLRESVHALKQIESAGVGDAGSPGDIGTQAKATAIASVVEQREQTNRKMIAL
                     LDRMYDDLSPTTAMRKQPRSPEETKLIERLIDQSSEMSDDTLRAFLDALA"
     gene            6736..7806
                     /locus_tag="VGR29_12830"
     CDS             6736..7806
                     /locus_tag="VGR29_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2074507.1"
                     /translation="MTHRLTASTPDQHVPALAAPIIEIRGLVKRYKGFTAVDGIDLEV
                     RAGEIFGILGPNGAGKTTTLEMIEGLRVPDEGSIRVAGIDAVTRNDDVRKIIGVQLQT
                     TALFPYLNAHELVELFARFYEVEDASSRATELLKLVNLNEKRDARVDELSGGQQQRLS
                     IALALVNTPKITFLDEPTTGLDPHARRSLWQTILDVRAAGTTVVLTTHYMEEAEVLCD
                     RIAIMDSGQVIACDTPEGLIRDLPQDAVVSATIAGHGSMSISAADIEAIPGVTGATIA
                     PDGTPPTITAQTRDVQATLVGLLQLAEQRRITLGELTSTRANLEDVFLSLTGRTYTQA
                     DDLPHGEETASAGKKRRRRGRS"
     gene            7909..8970
                     /locus_tag="VGR29_12835"
     CDS             7909..8970
                     /locus_tag="VGR29_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2074508.1"
                     /translation="MQSFVAMVVANLKMTVRNRQAIFWNLAFPAIFILMFGAVFGNGD
                     LDQFTVGIAGGESEFRAAVIDSMRNTDAFDIHEGSTDEELAALKKDDRSVVLVFPEEG
                     SDGAVEMYSSEASGPSGQIATGVTRSVVMEVAGQSSGISIEQKQISTLNASYIDWFIP
                     GILGMSLMNSGIIGISTAFVSFREKGILRRIKVTPFALWKFILARIVAALVISLVTSG
                     ILIGIGWLIWDLTVRGNPLLVIAVLVVVSMAMLAIGYAIAAFARNTETAATYANMVTF
                     PMMFLSGVFFPLGSMPDWLQPLISIMPLKYGVEALREPMLYGNGLGTIWQDLLILAAI
                     FVAFMAFAIRFFKWDATAR"
     gene            complement(9168..10217)
                     /locus_tag="VGR29_12840"
     CDS             complement(9168..10217)
                     /locus_tag="VGR29_12840"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEV2074509.1"
                     /translation="MTEPTVRVMIASPLEPEHIATIRRALPAHVELLHEPTLLPPTRY
                     VSDHHGPAEFRRTPEQERQWQEMAASADIAFEFPDSNRYPHAYAPNLKWVQTTSAGVG
                     QMVTRLGITPGEFIITTASGVHARPLAEFVFMVLLMAVKDHQRLAAGQQAHHWERFCA
                     DELSGKTLAIIGPGKIGQEVARIGRCFDMHPVALGRDVRPERAARIGVDRLFTRDELP
                     EMLGMADALVLCAPHTPETENIMDQAAFDALKPGVILVNIGRGPLVDEEVMLGKLKDG
                     TIRFAGLDVFRIEPLPADSPFWDLPNVIVNPHSASTSIHENGRIVEIFVHNLACFLDG
                     RFDEMRNVLDIARMY"
     gene            10499..11437
                     /locus_tag="VGR29_12845"
     CDS             10499..11437
                     /locus_tag="VGR29_12845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV2074510.1"
                     /translation="MQHAASRESSLTRFAWLSMAAAVATISLKSGAYLLTGSVSLLSD
                     ALESLVNLVAAIVALIALIVAAKEPDEEHTYGHAKAEYFSSGLEGVLVLFAALVIAVT
                     AVPRLMDPEPIEQVGAGLAVSVVASLVNGAVAWRLFRAAASYRSITLLASARHLITDI
                     WTSGGVLLGVTLVALTGWNRLDALVALAVAANIVWTGWSLVRKSMLGLLDTALPPEDL
                     QHIHDILARYRAEHGIETHALRTRAAGTRRFVSVHVLVPGDWTVQRGHRLLDEMEHDI
                     HAVLPETTMFTHLESLDDPASWDDVRLDRSTADAAQ"
     gene            complement(11532..12191)
                     /locus_tag="VGR29_12850"
     CDS             complement(11532..12191)
                     /locus_tag="VGR29_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074511.1"
                     /translation="MAEEEQHQRAGESGRRGRLARSSEGGARQRSRSVYAVLAVGIVA
                     LLSLLLIIYFSSDRDLPEQPICTTVDAESARQAVLEGRITRVTIAYDDTVIRPSAPKW
                     GPVLARLDYSDGQCANLPQGIVNQDDIYLLLGTITAFNDITENTQVEITYDRETSLQP
                     GLFTVPTPSPTPTEEPAATPVPSPSPSPEPTPTTEPDVPVGPILLPGSPEASPELTPG
                     V"
     gene            complement(12308..12610)
                     /locus_tag="VGR29_12855"
     CDS             complement(12308..12610)
                     /locus_tag="VGR29_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074512.1"
                     /translation="MFNRDNAIGIMLLGACTVVAAILIYAIVTGEQLRVDFPPYISWP
                     LGIAFVGLILYGLFSNFRDRRSSGRGHSWPNPQTGNKTLGDRFREWRKKDSDSFGR"
     gene            complement(12610..13680)
                     /locus_tag="VGR29_12860"
     CDS             complement(12610..13680)
                     /locus_tag="VGR29_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074513.1"
                     /translation="MEPRPQQPRQPSGQRSQSPGQSPPGGRSRQPASTEHYRARPAAE
                     PHQQVYPEGYDPSAVVPPPRTDYDYSPLDLAPPGQRRRRQFIAGAIGALSVVLVGALI
                     VFGWILLRDETPSDDENRAAIATQAPSGGDLAAAPGENAAGTPAVATEAPTTAPAPTQ
                     PASTDPVPPAPAGQAVATDQDSLIATLPNAAMLPPGFGEGADTVRTQEEVVNALGGSR
                     IAEQNLANWGWTANVERAFNNTAPEAGATSNITISLHGFKDAASAAQALPFYSDVLAA
                     NGYYDVPAPALGESARMLQLDQEDGGTLVALYVQQGPILYRVGGYALGGNPTQDVINV
                     ATQMLAGTQPQAIVWPESMRIT"
     gene            14125..14472
                     /locus_tag="VGR29_12865"
     CDS             14125..14472
                     /locus_tag="VGR29_12865"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV2074514.1"
                     /translation="MSNPNPETAMDTPTPGSYSRDDRMVSEAPATSRRTTDAPLARFY
                     MSGDLRAATGLSRTHLDFYLREGIILPTARTESGYLLFDDSEVAVAHQVISWREQGVS
                     IRDIRKRLHRDCE"
     gene            14478..15515
                     /locus_tag="VGR29_12870"
     CDS             14478..15515
                     /locus_tag="VGR29_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2074515.1"
                     /translation="MASSTLSPTILGLNDRPGRGARIAEAFALTGDPFPLDSHGDIFV
                     ATSVHGPAREELRRWIAEMLEDPNRNDRLAVVTGEEGSGKSHLLTVIAHEFASDARRR
                     LVILPGSPGQRTDAQILRAILSALGADPIGRTGLELRREIQGALRAVDQQGIQPGLLV
                     DGADFKGSHLELIRNLLRDTEGTGLWIVLFGTPDLQLRVSRRRSLRGLMGPAIPLGPL
                     PVADLDMVLDERIDAMRTDRTPTTLFPPGVRTTVHSWSEGNIAKLVGIAGACLLASAI
                     QGNEAVTPEIARQVARDLTIDEANGVRPVSTSSQEGNVVQTQIPLLLDGPPPRLSSTT
                     TQQALWKEDES"
     gene            16147..18321
                     /locus_tag="VGR29_12875"
     CDS             16147..18321
                     /locus_tag="VGR29_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074516.1"
                     /translation="MTPRRPDNREQRHIPSPRLDEPSAIRGDGMPVSTEGQLTGIVGD
                     ESASSGQSRSGEDTRRRGWFWHWNSIVTQFAPIIGLKGVGLLNSYTVWTDRREESPHR
                     GYAFPSQQSEAAFYGEDRNELITINKILVALDLIEIRKEMVPRTDEKGRRWKVPHNLY
                     RVKDHGDDFNLTANDVMRVVRLADKDRAVYRYIRRIFSPKFAPIDRDNIWHEILEELR
                     PTDVWQRLAARTERDETRASARSKAGHASRRTSDTETLFSLPDDSDSGAFEATAPASS
                     NDSATGDTNDSDTFAPTSDAITNNGSATSVAKSSNGSWRKPTSGDAPANRGKATSVAP
                     TSTTYYQELTTTTTGSEGKKSGEDTPAMAASIQMNGPGFVPDDRRDEDATVRLFDEAN
                     GRPSTAAARRHLKRIADDYADLARSAGVSGWALVGSAIDEAVSSGSAFVAPKRVREIV
                     ARWDRDGVPGMYVPKTQFGDPVASRGPQPVTSGPGAGHRSLQSVGLPGEGDPVDVWYA
                     TCHSLSPSLPQRVVEALRSGVSIVGYEAGDVMLQVSTDELARSIRDEWAELIQRKLGV
                     VLRRPVRILLGGPAPQDPDDPAPTPTSTPSPKPGTRRTGPIGSPPSARQPVPVFRIEE
                     CGMSSAQVWTSVLDDLRGSGAVPGAEIDTWLRDSELIGRDPESDAFIVGVPHALAERR
                     AARFRSSLETATMHIVGFDCEIEIVRTQAWLTAQDVSTGTGG"
     gene            18548..19024
                     /locus_tag="VGR29_12880"
     CDS             18548..19024
                     /locus_tag="VGR29_12880"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV2074517.1"
                     /translation="MTPNRTVDEQARDDAFRKRSGSHVERYIASDGREGYDDNSHRAP
                     TLLLTTMGRRTGKLHIAPLYFAEDGGRYIVIASKGGSDRDPQWYLNLVAHPEVGVQVR
                     DQVFRAIARTADADEKERLWPIMGSAYPFYNDYRRATDRDIPLVVLEPISAQGDSR"
BASE COUNT         3622 a         6046 c         5973 g         3483 t
ORIGIN      
        1 ccatgcagga agtcctcgac gccgccggcc cgttcatcgt cagccagtag cgcaccggtt
       61 gccaatcgct tttcacgcaa gaaaaagaac tggagtctct catgaccacc acacgcgaca
      121 agatgctcaa cagcctgctc acctcaacca tgaaccgccg tcgcatgctc atcaacgccg
      181 ggatcgtcgg cggtagcgtt gctgcgatgg gcaccctcgg atccgtcggg gctcagacca
      241 atgcgacgcc agcggccgac gcaggcgcag gtgtcgcctt ccagaacccg gtggaggtcc
      301 tcaatttcgc cctgacactg gagcatctcg aggcagcgtt ctatcgtgac ggcctcgccc
      361 agttcggcgt cgaaggcatc accgcccttg gcttccagca gtcggtcttc gattccctga
      421 acgagatcgc ccagcacgag gttacgcacg ttgataccct gacacaggtc atcaccgacc
      481 ttggtggtca accggtcgcc gaagcccagt acgacttcgg atacgccgac gccgctggtt
      541 tcctgacgac ggcccaggcg cttgaggaca ccggtgtctc cgcctaccag ggcgccgctt
      601 cctccctgat cgagagcgat gagctgctga ccgccgcact gacgatccac gccgttgaag
      661 cgcgtcacgc cgcctatctc gcgctcctga acgcaatgat ccccttcccg gaggccttca
      721 acccgacgct gacacctaac caggttcttg agattgctgg tccgttcatc gtcagccagt
      781 aaggctaact ccttgcattc cctatcccca gcacagcggg cgagatcaac gttgatcttg
      841 cccgctgtgc tgttgtgtct ggacgcgcag gcagtcatca tcacagtgcg gtgagcaggg
      901 gtgccccctc tttcagggca tcacaagatc accgtcagca aggcattcaa ggactactca
      961 ctgaaaagcg gccgttcatc ctggatcgtt gctggggccg gtgtgtcagt cgggtaggtg
     1021 agggatcggc accgtgaacg tggtcatcgg ctcctcgtat ttcgagatgc gcgtgaccac
     1081 ctcgacggtc cattcccctt cgaacggcac ctggacctgg ctggcatagt gcccggggtg
     1141 gccagccgat gtcgccgtgg atgtgatcgg gccaagtccc gcctcgggga gtgagagccg
     1201 tatctccggg tcgaccagaa gctgaatcgg gagtccgtcg gcgtcgtgga ccatcagcga
     1261 gatctcgttc tcgcccgtca tggcgggcgt gatggtgatc tccagcgacc catcaccgag
     1321 cgcctcagcc tcctcgaacg ggacagctgc caccgcctcc acaccatccg atccggcaac
     1381 cgccggattc tgcagcacca ggaacccggt cagcgaaagc actaccacga gcaggcccgc
     1441 ctcggtcagg accgtggtcc gcagccggtt ccaggccgag gaggcctccg gatcgcgctg
     1501 gacggatggc accaggcgca ggcggttcca caccgcgaga ccaaagggga tcaccacaag
     1561 cgagagcttg gcaatcagga tgcgcccata cgtcgtgtgg agcagggcat cccaggaccc
     1621 gagaatcttg aacgcaatta ccgccccgga gattgccagt gccgcgaagg cccagccggc
     1681 cagccaggag aagcgggcaa caacctgtgc ggcatcccct ggccctgggc ggttgccgga
     1741 cgatcgcgtg cggagtgcca cgtacagata tcgccccaac ccgagcagcc ccccgaacca
     1801 gatggctccg gccgcgccat gcacgaaatc gctgccatgc atgagccacg ttggagggat
     1861 gtccgcggta tggccaacga ccgtcagcga tccaagcgcc aacaggccgc ccagcgcgac
     1921 cgcccggcgc gtgcgcctgg ttgcatcgga tgagcgagcg aaatggatct cgatggctaa
     1981 tccggcgacc acgaagatga ccgcgcgcag ggtgaccgac agcggctcca cttcccacat
     2041 ttcgggatcg gccagggcac cgatgctctc acccacctgg cggatcgtgg ttagggggat
     2101 caatagcaga tgggcgatcg ccgaaaggat cgctccaccc gtcgccaggg agatggtccg
     2161 cccgtcgaca tcccggacaa cgaagacgcg gaaccagatc aggccgactg tcgagagcag
     2221 ggagagatag gcgatgccct ggacgatgct cgctattggc gtcacatccg atgagctggt
     2281 ggcaacctcg accggtgcgc cgctttcctc tccgatggag aaggtgaggg cgccggtgat
     2341 cggatgggag tcgagcgaga cgacacgcca gtcgactaca taggtaccat ccgccagatc
     2401 gcccggcaac gcgatttcga gggtctctcc cgtcgccggc gtctccagcg tcgcaaccag
     2461 accgtcactg ttgaacaacc ggaatgcctc ttgcacaggt tcgacggctt cggtgaaggt
     2521 caacgtcacc tggtctggct gggtctccag cagagccccc tcggccggag tggtgttgac
     2581 cagctcggcg tgcgccgaga cctgggaggg cgtgaggagg ccaccaacaa ggaacgcccc
     2641 caggacgacg atgatcgtca gccaccggga gcgccccacc tgaagacctg ttgcccgcga
     2701 aaccgccatc actactgatc cttcaaagcg gggacacgtc cacagcgaca ccccgcacac
     2761 atgatgaacg gtaccagatg cgaagctcgg taaccggcga aatgccggtc ccaacgggaa
     2821 tgcgtgcgag accgcacaga aggtagagtc accttgcggt caccgctgat ggagaccggg
     2881 gcagattctg cgcctgcccc tcgccgttca atgcacagat ggcgattgat tctggcccgt
     2941 gccggtcggc tactcttcac tctcctcctc cggcgcggcg atcgtcacgc ccggagccgg
     3001 ggcctcgagt tcgtcatcgt cctgtccctc ttccgggatt tgcacccagg cagtctcgtc
     3061 ctcctcgcag gtctggatga tcgggaagta gagcgtttcg ccctcgccat cctcaggcag
     3121 ttgcacggag agcaggaact ggtcgtagaa tccatccggc agcggctcct tggcggtata
     3181 aatgatctcg ctgacgcgac cgccctcacc atgggcgccc tcagccggcg tcgcggctgg
     3241 cgttgcctcg ctctccatca ccttctcgac cgtccagccg taattcatgc ccggtgcgat
     3301 gctggtgatc tcctcgggta cggagatgga gatcttcgtc gttggggatc cctcgcatcc
     3361 atgcgagaac ccaaccgtca gcagggtgtg agctcccgct gccgtctcat ccgccgtgat
     3421 cccgacatgg gcacttactg gcgccacccc tgccacagca agcagcgccg cccccccaac
     3481 acccagcgcc aattggcgaa cacgccgcat tgcctcattc gtgagcacac ctcgagtctc
     3541 tgactatgga ctaccggagt ggcaagcacc acctcgcccc ctacaataga tcgactctga
     3601 cacggcggca tggccgacgc aatacactca gggggcatca acctgtcgag gggacgggtg
     3661 cgaccaggtc atcgaagtgc cggcgaaagg gagctatggc ttgagttcag tcgcggggtt
     3721 cgtcctcagc ctggcttcga gacgctcata gtcacccaga tggacgcgca tgagcatctt
     3781 ccataacctg ccatgattcg gggcggagaa gtgcagcaac tcgtgaacga tcacatagtc
     3841 accgagctcg cggtccatgc ctgtcagctc atcattgaag ttcagccggc cattggtaga
     3901 gcacgacgcc cagcagaatg aagtccttgt acttggctcc ttctctcgct ccacgaatcg
     3961 agcatgcagc gtcccacaac catgattcga gcgatttctg tttctcaaca acagccacag
     4021 gtaccccttc aacaacacgg gatcaattca gacactcgtg cgagagacga ccggttgccg
     4081 tccagttcgc gacggttggg ttggcgcaaa gatccaaagt gattgtatcc aattggtcgc
     4141 ggacatgccg ccgcacggca agcgccccct ttcacactgc cactatctcc ctataccgac
     4201 aaattggcca tctaggcagc ctaagtcaag atcgacgctt aggctgccag atttcgcccg
     4261 cgctctgaac aacttggcag cctgaagaaa tccctgctat gtcggcgaaa accgcctcaa
     4321 tggagcctta ggttgccact ggccaaatta tttcatttgg tcatctaacg cgcgctccac
     4381 cggacccacc agctcattga agagccgatc gagggtcagc tgtggatcgg tcgtcaggcc
     4441 ggagtgaacg ggggagggct ggatgatcgt gctgctgggg gaggtgatcc agtggaaccg
     4501 ctcggcgggc tccagcgcag ccatcggacc agccgcagga tcgcccgcgg cgaccgtctc
     4561 cagcgcgaca agttgctcgc ggatgatctc cggatgtacc cccggtgcga ggaccgcgag
     4621 tttttcctca tcaagatccg tccggaccgc gaggaagcgc cgtgaccggg agagcatcgc
     4681 cacaccgacg ttgaagcatt cgccacgctc gacccggggc acgagccgca ggaccgaata
     4741 gaagtaggac acacgatccg ggctattcac ggcggcggcc ccgtgtcgcc cggtgcggat
     4801 cgatcgcgcc gacaccttcc gaggtgcggg agtgctcgcc ctcctcgatg aacgggcgtg
     4861 gcgcctccag ccgagcggtg aaataggcca tgtaggcctc ccgctgctgc tcggcgtttc
     4921 cgatctcctg gttctccggc agccaggcct cggggatgct gccgacgatg ctccagagca
     4981 cggactcatc cagccgggcg gccatccgcg catcggcgtc gaccagcgat cccgccaccg
     5041 gcaggagcac atgatccttg atcgcggcga aggatcggcg accctgcgcc gcgggatcct
     5101 gccagcgatg atgcggataa atcgcggcgc catgatcgat cagccagagc ccgtcgtgcc
     5161 agcgcagcat gttcgggttc cgggtggtgc gatcaacatt cgcaatgtac gcatcaaacc
     5221 agacgatatc ggcggcaagc accgggtcaa ccggcgtccg cgtcgcgggg tcgaacggca
     5281 gtgcgcccgg gaggaagtcg aggccgaggt tgaggccaac ggatcgggca agatgatcct
     5341 gaatctcgag gtccggctcg ccgatgctca gctctacggg aagatcgaca agcaccagct
     5401 ccggaaccct gagtcccagc ttgcggccga tctccccggc aagcagctcg gcggtgagtg
     5461 tcttcggccc ctgggcagcg ccatggaact tcaccacgta aaggccgtcg tcgtccgcct
     5521 caaagagacc gggaacagag cccccctccc gcaaggggag gacgaacctg gtagcggtga
     5581 cctcccgtac ctccggggtc accagtggga gatctgtcat cgtcgtcgca tatcctgcgt
     5641 gaatggtgat cgcgtctctg gcccgcagta tcccatacgc cgagcgctcc ggacggcgag
     5701 atgtgggacg cctgtcgaca tcctgcaccg ggaggatcgt caggggagca gcaagacggg
     5761 gcgggcggct ggccgccccg tcttgtgaca tacgctacgc gagcgcatca agaaacgccc
     5821 ggagtgtgtc gtccgacatc tccgacgact gatcgatcaa ccgttcgatc agtttcgttt
     5881 cctcaggaga ccgcggttgt ttcctcatag ccgtggtcgg cgagagatca tcgtacatcc
     5941 tgtcgagcag cgcaatcatt ttccgattcg tctgctcccg ttgctcgacg acggaggcga
     6001 tggccgttgc cttcgcctga gtcccgatat cacccgggct gcctgcgtcg cccaccccgg
     6061 cgctctcgat ctgcttgagc gcgtgcacgc tttcgcgcag gtacgcggtc tccgcgatca
     6121 gcatatcgat gcgctcgagg atatcgctcg ccgcgaccgg ccgcgaggat ggcgtttgcg
     6181 atgccggtgg tccgctgtta acgccgaacg gcgcggtcgt cgttgtgtcc tggtcctgtg
     6241 attgggtctg catgatcgtc tcctcgccct gggggtccgg aggacgcgtc agttctatgg
     6301 tatccgggtc gatgaagcgc gccctgccgt gcttgacaag acccaaggct cttttgcgat
     6361 aggttgactg caaggagttt ccggcgccgt caacaacgcg aacgtttttt gtgatgggtg
     6421 tcctcccctg tcatatcaac gccgatgaag ggaaccgacc gttttttgta atgggtgtca
     6481 tccccgcggt tccatcgtat cgtagcatgc acctttgtcg aatggacacg tcagtcgttg
     6541 ttcgcaggcg taagtacggt cgacgccgag gcgtcaggag gccgggggcg agccagtcga
     6601 tgcgttgggc gttgcggtga agaagcccgg agctccttcc gatgtgcgcg catcggtgcg
     6661 ggtaccattg cagggacaag gggtcgcaag ccggcctcgg gcccttcctc tcgccttcac
     6721 ccggagacct ccccgatgac ccatcgcctc acggcgtcga cacccgatca gcatgtcccg
     6781 gcacttgccg cacccatcat cgagatccgg gggctggtca agcgctacaa gggcttcacc
     6841 gctgtcgacg ggatcgatct cgaggttcgc gccggggaga tcttcggcat tctcgggccg
     6901 aatggcgccg ggaagaccac cacgctggag atgatcgagg ggctccgtgt accagacgag
     6961 ggaagcatcc gcgtggcggg gatcgatgca gtgacccgca acgacgatgt ccgcaagatc
     7021 atcggcgtgc agctgcagac gacggcgctc tttccctacc tgaacgccca cgagctggtg
     7081 gagctcttcg cccggttcta cgaggtcgag gacgcctcgt cacgcgcgac cgagctgctt
     7141 aagctcgtca acctgaacga gaagagggac gcccgcgtcg atgagttgtc gggtggccag
     7201 cagcaacggc tcagcatcgc ccttgcgctc gttaacacgc cgaagatcac ctttctcgat
     7261 gagccgacga cgggtctcga cccgcatgca cgccgcagcc tgtggcagac gattttggat
     7321 gtccgcgccg ctggcacgac ggtcgtgctg acgacgcatt atatggaaga agctgaggtc
     7381 ctctgcgacc ggatcgcgat catggacagc ggccaggtga tcgcctgcga cactccggag
     7441 ggcttgatcc gggacctgcc gcaggacgct gttgtcagcg caacgatcgc cggacatggc
     7501 tccatgtcga tcagcgcagc ggatatcgag gccattcccg gcgtcaccgg cgccaccatc
     7561 gcaccggacg ggacgccccc aacgatcacg gcgcaaacgc gcgatgtgca ggcaacactg
     7621 gtgggtctgt tgcagctcgc ggagcagcgg aggatcacgc tgggcgagct caccagcacc
     7681 cgcgcgaacc tggaagatgt cttcctgagt cttaccggcc ggacctacac gcaggccgac
     7741 gacctcccgc atggagagga aaccgcatcg gctggcaaga agagacgccg acgcggccgg
     7801 agctgatcag ctcgcgatca ccagggatta ggtcctctta acccttcccg gcctctcctt
     7861 cggtcctacg ttatttctga agattcgtct cccgggagcg cacatcacat gcaatcgttc
     7921 gtcgccatgg tcgtcgccaa cctgaaaatg accgttcgca accggcaagc catcttctgg
     7981 aatctcgcct ttccggcgat cttcatcctc atgttcggcg ccgtcttcgg caacggtgat
     8041 ctggaccagt tcaccgtcgg tatcgcgggc ggggaaagcg agtttcgtgc tgcggtcatc
     8101 gactcgatga ggaacaccga cgccttcgat atccatgagg gcagcaccga cgaagagctc
     8161 gccgccctga aaaaggatga ccggagcgtg gtgctggtct ttccggagga ggggagtgat
     8221 ggagcggtcg agatgtactc ctcggaggcg agcggcccct ccgggcagat cgcgaccggc
     8281 gtgacgcgca gcgtcgtcat ggaggtggcc ggtcaaagca gcgggatctc catcgagcag
     8341 aagcagatat cgacgctcaa cgcgagctac atcgactggt tcatcccggg aattctcggc
     8401 atgagcctga tgaactcagg catcatcgga atctcgaccg ccttcgtctc attccgcgag
     8461 aagggcatcc tgcgacggat caaggtgacg cccttcgcgc tctggaagtt catcctcgcg
     8521 cggatcgtgg cggcgctcgt catctccctg gtcaccagcg gcatcctgat cggaatcggc
     8581 tggctgatct gggatctcac cgtacgcggc aacccgctgc tggtcatcgc ggtcctggtc
     8641 gtcgtctcga tggcaatgct ggcgatcggc tacgcgatcg cagcctttgc ccgcaacacc
     8701 gagacagcgg caacctacgc gaacatggtc accttcccga tgatgttcct ctccggcgtc
     8761 ttcttcccgc tcggatcaat gccggattgg ctgcagccgc tgatcagcat catgccgctc
     8821 aagtacgggg tcgaggccct gcgagagccg atgctctacg gcaacggcct cggcaccatc
     8881 tggcaggacc tgctcatcct cgccgcgatc ttcgtcgcct tcatggcctt cgccatccgc
     8941 ttcttcaagt gggacgcgac cgcaagatag agagcagccg tcggcttggg atgtcaccat
     9001 catcccgctg aaacgctatc gccccacgcc ttcgacgttg cccgtatccc ttccagaacg
     9061 tgcatcgacc actccgtcat cctgagcttg tcgaaggatc ccccgcggag cgcgttgggt
     9121 cacacaccga cgcgaagtag caatgcccgt ccggtgattg tgcccatcta atacatccgt
     9181 gcgatatcca gcacattacg catctcatcg aaccgcccgt cgagaaaaca ggcgaggttg
     9241 tggacgaaga tctcgacaat ccgcccgttc tcgtggatcg atgtgctggc cgaatgggga
     9301 ttgacgatca cgttcggcaa atcccagaag ggggaatcgg ccggcagggg ctcgatacga
     9361 aaaacatcca gcccggcaaa gcggatggtg ccgtctttca gcttcccaag catcacctcc
     9421 tcatccacca acggaccccg gccgatattg acgaggatta ccccgggttt gagcgcgtcg
     9481 aatgcggcct ggtccatgat gttctcggtc tccggcgtat ggggcgcgca gaggacaagg
     9541 gcatcggcca tgcccagcat ctccggtagc tcatcgcgcg tgaagagccg atcgacgccg
     9601 atccgggctg cccgctcggg acggacatcg cggccaaggg cgacgggatg catgtcaaag
     9661 cagcggccga tccgggccac ctcctgcccg atcttccccg gaccgatgat ggcgagggtc
     9721 ttgccggaga gttcgtcggc gcagaagcgc tcccagtgat gcgcctgctg cccggcagcc
     9781 agccgctggt gatccttgac ggccatcagc aagaccatga agacgaactc ggccaggggc
     9841 cgggcatgca cgccgctggc ggtggtgatg atgaactcac ccggcgtgat gccgagccgg
     9901 gtcaccatct ggccgacacc ggcactggtc gtctggaccc atttcaggtt tggtgcgtag
     9961 gcatgtgggt aacgattgga gtcaggaaac tcgaaggcga tatcggcgct ggcggccatc
    10021 tcctgccact gtcgctcttg ctcgggcgtg cgccggaact ctgcaggacc gtgatgatca
    10081 gacacatagc gcgtcggcgg gagcagcgtg ggctcgtgga gcagctcgac gtgtgctggc
    10141 agggcacgcc ggatcgtcgc gatgtgctcg ggttcgagtg gtgacgcgat catcacacgc
    10201 acggtcggct cagtcatcag tccctctcca aacgctcatg ttgctcattg ggcagggaag
    10261 tctacccgtg attcactgct ccagacgaac cggatctctt tccacctgta tattcgctgc
    10321 atctctccca gcatggggca ttcgcccagc ccattgtcca cctccttgca gcagggggat
    10381 gagatggagc cgaccccata cctttcttag tcccggctaa acgaagtaag ctcagtggtc
    10441 atcgagctcc agtgtcgagc cttggtgccg aagctgatgc ccgaggagca tttgtctcat
    10501 gcaacacgct gcatcaagag aatcctcgct cacgagattc gcttggctat caatggcggc
    10561 cgccgtcgcg acgatctccc tcaaatcagg ggcgtacctc ctgacggggt cagtcagtct
    10621 cctttccgat gccctggaat cgctcgtcaa cctcgtggcg gccatcgttg ccctgatagc
    10681 gctcattgtc gccgccaagg agccggacga ggaacatacc tacgggcacg ccaaggcgga
    10741 gtatttcagc agcggccttg aaggcgtgct ggtgctgttt gcggcactgg tgatcgccgt
    10801 gactgccgta ccccgcctga tggacccgga gccgatcgag caggttggcg cgggcctggc
    10861 cgtttcagtt gtggcctcgc ttgtcaatgg agctgtcgcc tggcgtctct ttcgtgcagc
    10921 ggcatcctac cggtcgatca ccctgcttgc cagtgcccgt cacctgataa cggatatctg
    10981 gacgtcgggc ggcgtactcc ttggtgttac cctggtcgcc cttaccggat ggaaccgtct
    11041 tgatgcgctg gttgccctcg cggtagccgc caacatcgtc tggacgggct ggtccctggt
    11101 acggaaatcg atgctgggtt tgctggatac ggcgcttcca ccggaagacc tgcagcacat
    11161 ccatgacatt cttgctcgct atcgcgcaga gcacggtatc gaaacccatg ccctccgcac
    11221 ccgcgccgca ggaacccggc ggttcgtctc cgttcacgtg ctggtaccgg gagactggac
    11281 ggtgcagcgg ggacatcgcc tgcttgacga gatggagcac gatatccatg ccgtcctgcc
    11341 ggagacgacg atgttcaccc acctggaatc gctcgatgac ccggcgtcgt gggacgatgt
    11401 gaggctggat cgatcgaccg ccgacgcggc tcaataagat cgggcccacc cagcgcttca
    11461 aatcttgagt ggttcgcctt gggatgaccg tcagtgactc acctgtccca acggcgtgat
    11521 ggcgtgccgt ctcagacgcc gggtgtgagt tcgggagatg cttccgggct cccggggagc
    11581 aggatcggtc cgaccggaac gtccggctca gtcgtgggtg tgggctccgg tgaaggcgaa
    11641 ggcgagggga caggtgttgc cgcaggctcc tccgtcggtg tgggcgatgg tgtcgggact
    11701 gtaaagaggc ccggctgcag gcttgtctcg cgatcgtagg tgatctcgac ctgggtattc
    11761 tcggtaatgt cgttgaacgc cgtaattgtg ccaagcaaca ggtagatgtc atcctggttc
    11821 acaattccct gcggcaggtt tgcgcactgg ccatcggagt aatcaagccg cgcgaggaca
    11881 ggaccccatt tcggggcaga ggggcgaatc actgtatcgt cataggcaat cgtcacccgg
    11941 gtaatcctcc cttcgaggac ggcctgccgg gcgctctccg catcgaccgt ggtgcagatg
    12001 ggctgctcgg gaaggtcccg gtccgacgag aagtagatga tcagcaagag ggagagcagg
    12061 gcgacaatac cgacggcaag gacagcgtag acggatcgtg atcgctgacg tgcgccaccc
    12121 tcggaagacc gggccaaacg accccgtcgg cctgattcgc ccgcccgttg gtgctgttct
    12181 tcctccgcca tcggcccggt ctctccttcc acgaccattg gccgcacatg acagggcacg
    12241 accacaccac gtattcccac ccgcctgccg gcatcatcct atgcagaccc cgcctgtgca
    12301 acgctgatta ccggccgaaa gaatcgctgt ccttttttct ccactcgcgg aagcgatctc
    12361 caagcgtctt gttgccggtc tgcgggttcg gccatgagtg gccgcgacca ctcgatcgcc
    12421 gatcacggaa gttgctgaac agtccgtaga ggatcaaccc gacgaaggcg ataccgagtg
    12481 gccagctgat atagggtggg aaatcgaccc gcagctgctc tccggtgacg atcgcataaa
    12541 tcaggatcgc cgcaaccact gtgcaggcgc caagcagcat gatcccgata gcgttgtcgc
    12601 ggttgaacat caggtgatcc tcatcgattc cggccataca atggcttgcg gctgggtacc
    12661 ggcaagcatc tgcgtggcga cattgatgac gtcctgggtt ggattgccac cgagggcgta
    12721 gccaccgaca cggtaaagaa tagggccctg ttggacatag agcgcgacga gggtcccgcc
    12781 gtcctcctga tccagttgca gcatgcgggc gctctcaccc agagccggcg ccgggacgtc
    12841 gtaatagccg ttcgcggcaa ggacatccga gtagaacggc agggcctgcg cggcggaggc
    12901 agcatccttg aacccgtgca ggctgatggt gatgttggaa gtggcgccag cttcgggcgc
    12961 ggtgttgttg aacgcgcgct ccacattcgc ggtccaaccc cagttggcga ggttctgttc
    13021 ggcgatgcgg ctaccaccca gggcgttcac gacctcctcc tgggttcgga cagtatcggc
    13081 gccctcgccg aatcccggtg gaagcatggc agcgttcggc agggtggcga tcagggaatc
    13141 ctgatcggta gcgactgcct ggcccgccgg agcgggaggg acagggtcgg tcgaggcggg
    13201 ttgggttggc gccggagcgg tggtcggggc ttcggtggct acggcgggag ttccggcggc
    13261 gttctcaccg ggagccgccg ccagatcgcc gccggagggg gcctgtgtcg cgatcgctgc
    13321 gcggttctcg tcgtccgaag gggtttcgtc ccgcaagagg atccatccga agacaatcag
    13381 cgccccaacg agcaccacgc tgagcgcacc gatcgcgccg gcgatgaact ggcggcggcg
    13441 acgctgaccc ggcggtgcga gatcgagagg gctgtagtcg taatccgttc gcggtggggg
    13501 gacgacggca gaagggtcat aaccttctgg atagacctgc tggtgaggtt cagcagccgg
    13561 ccgcgcccgg taatgctcgg ttgatgccgg ttgccgggaa cggccacccg gcggggactg
    13621 gccgggcgac tggctccgct gccccgacgg ttgacgcggt tgttgcggtc ttggctccat
    13681 agagtctttt ctcaggtcct ttcgtagcaa tcggtggtag aggccgcgga atgccgtgtg
    13741 tgcccggatg aaacgctgcg agcgagcgag gccgggaggg agggtgatgc gcgcagggcg
    13801 aatttcgggc caaaggacat cgcggattct gccccgatgc cctgtccaga gggaagtata
    13861 ccgggcaggc cgcgtgtcgt ggcgaaacgc tcccaccccc gcatacgccc cggcatcgct
    13921 taccgcgccg agccgtatat agatggaacg cgtgtgcagg gattgcgttt cctcgtgtca
    13981 ccggctcatg ccggcgaacg atagggtatt tacattcgcg ccccaaccgc gtaggatact
    14041 ggtatgcaat tgcagagtgc cttggcatcc ggacgctggc acgcatcttt actcaccgga
    14101 cgccctccat ttcaaggagc ttcaatgtcg aatccgaacc ctgagaccgc aatggacact
    14161 ccgactccag gatcctacag tcgcgatgac cgcatggtaa gcgaagctcc cgcgacctca
    14221 cgccgtacca ccgatgcacc gctggctcgc ttctatatgt cgggcgatct ccgcgccgct
    14281 accggcctca gccggaccca tctcgatttc tatctgcgcg agggcatcat cctccccacc
    14341 gcccgcaccg agagtgggta tctgctcttt gatgacagcg aggtcgccgt ggctcatcag
    14401 gtcatcagct ggcgcgagca gggggtcagc attcgcgaca tccgcaagcg cctccaccgg
    14461 gactgtgagt agtcccgatg gcctcctcga cgctttcccc cactatcctg ggattgaacg
    14521 atagacccgg acgcggagcg cggattgctg aggctttcgc cctgacgggt gatccctttc
    14581 cgctggacag tcatggcgac atcttcgtcg ccacctccgt gcatgggccg gctcgcgagg
    14641 agctgcggcg ctggatcgcg gagatgctgg aggacccgaa ccggaacgac cgcctcgcgg
    14701 tcgtcacagg ggaagagggc tccggcaaat cgcacctgct taccgtgatc gctcacgaat
    14761 tcgcgtccga tgcacgccgc cgccttgtca tattgccggg ctcgcccgga cagcggacgg
    14821 acgctcagat cctgcgcgcg atcctctccg ccctcggggc agacccgatc ggtcgcaccg
    14881 gtctcgagct gcgccgggaa atccagggcg ccttgcgtgc cgtcgatcaa cagggcatcc
    14941 aaccggggct cctggtcgat ggcgcagatt tcaagggctc gcacctggag ctgattcgca
    15001 atctcctccg ggacaccgaa ggcacgggcc tctggatcgt gctctttggg accccggatc
    15061 tccagcttcg cgtgagccgc cgccgctcac tccgtgggtt gatgggcccg gcgatccccc
    15121 ttggaccatt gccggtggcg gatctcgaca tggtgcttga cgaacggatc gacgcaatgc
    15181 gtaccgaccg cacgccgacg acgctcttcc ccccgggcgt gcgcacaacc gttcacagct
    15241 ggtcagaagg aaacatcgcg aagctcgtag ggatcgctgg agcgtgcctg cttgcttccg
    15301 ccatacaggg aaatgaggcc gtcacaccgg agatcgcccg ccaggtagcg agggatctca
    15361 cgatcgatga ggccaatggt gtacgtcccg tcagcacgtc cagtcaagag gggaacgtcg
    15421 tccagacaca gatccccctg ctgctcgacg ggcccccgcc gagattgtcg tccacaacga
    15481 cacagcaggc gctgtggaag gaggacgaat catgatagcc ggggcaggat taagcgtacg
    15541 tacagagcgt cggaacgggc gagcaggacg tatgctggaa gctgcggtga gtgtaccggg
    15601 tagattcgtg tcgggcttcg atcgcattcg catgagccga aagggacagg cggctgcgat
    15661 cggtgccgga acgccaaacg gcagcgacag gcgagcaatg tgctcaggtg atgagaagga
    15721 aagggagggt tgccagcacg cagagtgcat cgtcgagcgg atgcatcatg ggagattcgg
    15781 gtaccgcgat atgtgcgcca acacatctcc ggttagggaa tcgtcccttc gtccattcga
    15841 aacgacgata cgtatcagca agaccggcag gccaatgacg gttgcagaca aagaaaaaga
    15901 ccggtgcgcc aacaccggac ttcttcgcaa agtgtcattg gttgtcacat tcgggattcg
    15961 atcggaccgt cgccaaaccg cctgatcagg attccgaacc ggagccgctt ttttcatccc
    16021 cgccaaggga gggttctgtg tacccaagcg acgttacgag gattctagtc acaccacctg
    16081 cgatgtgtca agaggctgcg tgcctcaccg tcacagttgc gctacgcccg tgggcgcgat
    16141 agcgtcgtga cacctcgacg accagacaac cgcgagcagc ggcacatacc ttcaccccgg
    16201 ctggatgagc catcggccat ccggggcgac ggcatgcccg tgagcacgga aggccaactt
    16261 accggtatcg tgggcgatga gtccgcaagc agcggccaaa gccgctccgg tgaggacaca
    16321 cgacgccgcg gctggttctg gcactggaac agcatcgtca cccagtttgc cccgatcatc
    16381 gggctcaagg gcgtcggcct gctcaactcc tacacggtct ggaccgatcg ccgcgaggag
    16441 tcgccacacc ggggctatgc cttcccctct cagcagagtg aggcagcctt ctatggagaa
    16501 gaccggaacg agctgatcac gatcaacaag atcctggtcg cgctcgacct gatcgagatc
    16561 cgcaaggaga tggtgccgag gacggacgaa aagggccgcc gctggaaggt cccgcacaac
    16621 ctctaccgcg tgaaggatca cggcgacgac ttcaacctca ccgccaatga cgtcatgcgc
    16681 gtggtgcggc ttgccgacaa ggaccgcgct gtctaccggt atatccgtcg catcttctcc
    16741 ccgaaattcg caccgatcga tcgtgacaac atctggcacg agatcctgga agagctccgg
    16801 ccaaccgatg tctggcagcg gcttgcggcc cgcaccgagc gggatgaaac ccgggcgtcg
    16861 gcacggagca aggccggtca cgcctcccga cgaacgtcgg acaccgagac gcttttttct
    16921 ttgcctgacg acagtgacag cggggcattc gaagcgacag cacccgccag cagtaatgac
    16981 agcgcaactg gcgacactaa tgacagtgac acatttgctc caacctctga tgcaattacc
    17041 aacaatggtt cagcgacctc tgttgcaaag agcagcaatg gttcatggcg gaaaccgaca
    17101 agcggtgatg ctccggccaa cagaggtaag gcaaccagtg ttgccccgac cagcactacg
    17161 tattaccaag agttaacaac gacaaccacc ggtagtgagg gaaagaaatc gggtgaagac
    17221 acgccggcaa tggcagcttc catccagatg aatggacctg gttttgtgcc ggatgaccgc
    17281 cgcgacgaag atgcgacagt gcgattgttc gatgaggcga atggacggcc ctcgaccgcg
    17341 gcggcgcggc ggcacctgaa acgaatcgcc gacgattatg ccgatctggc ccggtcggcc
    17401 ggtgtctccg gatgggcgct cgtcggcagc gcgatcgatg aagccgtctc ctcggggagc
    17461 gccttcgtcg ccccgaagcg cgtgcgagag atcgtcgccc gctgggatcg cgatggtgtg
    17521 ccagggatgt atgtcccgaa gacccagttc ggtgaccctg tggcaagccg tggtccgcaa
    17581 cctgtgacaa gcggaccggg tgctggtcac aggtctcttc agtcggtcgg gttgccgggc
    17641 gagggtgacc cggtcgatgt ctggtacgcg acctgccact cactctcccc ttcgcttccg
    17701 cagagagtgg tcgaggcgtt gcggtcggga gtctctatcg tcgggtatga ggctggagac
    17761 gtgatgctac aggtttcgac cgacgaactt gccaggagca tccgcgatga gtgggcggag
    17821 ttgatccagc gcaagttggg tgtggtcctt cgccggccgg tgcgaatcct cttgggtggg
    17881 ccggctccgc aggacccgga tgatcccgct ccgactccga ccagcacgcc ctcaccaaag
    17941 ccgggtacgc gacggaccgg accgatcggg tcaccgccat cggcccgtca gccagtgccg
    18001 gtcttcagga tcgaggagtg tggcatgagc agcgcgcagg tctggacctc cgtgcttgat
    18061 gacctgcggg gctcgggggc ggttccggga gccgagatcg atacgtggct ccgagactcc
    18121 gagctgatcg gtcgcgaccc ggagagtgac gccttcatcg tcggtgtccc gcacgcactt
    18181 gccgagcgtc gtgcggcccg gttccgctcc tcgctggaga ccgcgacgat gcatatcgtg
    18241 ggctttgact gcgagattga gattgtccgc acacaagcat ggctcaccgc acaggacgtc
    18301 tccacaggta ccggtggata ggaacctcgc ggatcacctg gaatcgagcg gccggtctcg
    18361 ctcatagctt gtcgtggagt cccgcagggt cgggatacgg tcgacccgta cgggtcacaa
    18421 tccctcctga gggagcgcag cagctccctc aggagggtag gctggccgga gtggcccagc
    18481 gtaagctgcg gtaggagtag catcgcggag tggcatcgac gcactcgtta ctgagatgga
    18541 gaccaccatg accccaaatc gaacggttga cgaacaggct cgcgatgacg cgttccggaa
    18601 gcgctcgggg agccacgtcg agcggtatat cgccagcgat gggagagagg ggtatgacga
    18661 caactcccac agggcgccga cgctgctgct gacgacgatg gggcggcgga ccggtaagct
    18721 tcacatcgcg cctctctact tcgcggagga tggcgggcga tatatcgtca ttgcgtcaaa
    18781 gggtggctcg gaccgggatc cccagtggta cctgaatctg gtcgcgcatc ccgaggttgg
    18841 cgtgcaggtt cgcgatcagg tattccgggc aatcgcacga acggccgatg cggacgagaa
    18901 agagcgcctc tggccgatca tggggagcgc ctatcccttc tacaacgact accggcgggc
    18961 gacggaccgt gacatccctc tcgtcgttct ggagccgatc tcggcgcagg gcgactcccg
    19021 atagaccgca tctcgcgagg aagaattgtt ccggccttgg ctcactggcc cggccaccgg
    19081 tcatcccgaa agaggcgtcc tgtgccgtgg catcagcgcg gcac
//
LOCUS       DASYWM010000325         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_524712, whole genome shotgun sequence.
ACCESSION   DASYWM010000325 DASYWM010000000
VERSION     DASYWM010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524712"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..966
                     /locus_tag="VGR29_12885"
     CDS             52..966
                     /locus_tag="VGR29_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV2074518.1"
                     /translation="MHEAAQVAQVTQVKHALPGTLGTSTNPDDLEVIGEGFSSLILAA
                     GDTVFRVPRHREAADRQAAVVRVLPELQRLLPVHISAPTVSVPASATLPFGAVAFERL
                     PGIPMRSGYAGPSIAGALGRLLAALHAIRPHHLSIPVRGRAGVDRARHVAMDAALAFL
                     RETLSAPDYVHIVRWWASYRGFRRLPLPTPALVHGDLWYGNILVDPGHTRILGLLDWE
                     EMAFDDPAQDFATLRHSGNAFSDDALAAYAHAGGAIDVGLLARREWHWECREVIGIAS
                     AIKTGDTAEIEETLDKLMSGPVMRSRRR"
     gene            complement(1093..2490)
                     /locus_tag="VGR29_12890"
     CDS             complement(1093..2490)
                     /locus_tag="VGR29_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074519.1"
                     /translation="MSRLTFLTVIVALVSSMLPRVAPALAQGDTADEQNAATAAYDIS
                     VLEAFSSYNAIYDRMHPDAQVFIPREAVVEWYENDFGPKQPQPITVTDVQMVEWVWPV
                     TGKTYPYTAEVAFEQSFGDGTSITDVVRLVQDDAGSWRWFFGRSREFVDTQIARYADV
                     ESVAPTAPSSSAQSSAGSSRCELVELYPGYPGYRGYITGLVGPGDHECLSDLEQMDPS
                     FSRETQDLENSLAASRLSISGPMNVWTWENWMAIEAERGLQPTCYSCLLVDASGRGPE
                     PNGAFIDPSDSRLLIGTSLSRFTGDTSTSSRLLQDHFLSSGTDNYFAAQFEASVAKEQ
                     GLPADAHLRALASLVANRPHLNSYELLATYRELMQVLYTPGASGKFHNLNLDLLYQVL
                     MGQGGYVPTPSTAHPNDQVFTIFLGFDALAPIMDSASYSAARSQLEVAADTWLIEVGT
                     NPTLSFAAYLRSEGW"
BASE COUNT          591 a          911 c          874 g          568 t
ORIGIN      
        1 cccccccccc cctctctcgt tccagcctga agcactggag gcaaaaaggc gatgcacgag
       61 gccgcccagg tcgctcaggt cacacaagtc aagcatgcgc tgcccggaac cctgggcacg
      121 agtacgaacc ccgatgacct ggaggtgatc ggcgaagggt tcagttccct gatcctcgcg
      181 gcgggcgaca cggtcttccg cgttccccgt caccgcgagg cggcggatcg acaggcagcg
      241 gtcgtgcgtg tcctgcccga gcttcagcgg ttgctcccgg tccacatctc cgcaccgacc
      301 gtgagcgtcc ccgccagcgc cacgctcccc ttcggtgcgg tcgcgttcga gcgcttgccc
      361 ggcatcccga tgcgttcggg ctacgccggt ccctccattg ccggggcgct gggccgcctt
      421 ctcgccgcct tgcacgcgat ccggccccat cacctctcca tcccggttcg cggccgtgcc
      481 ggcgtcgacc gggcccgcca cgtcgcgatg gacgcggctc tcgcctttct tcgcgaaacc
      541 ctctccgctc cggattatgt ccatatcgtt agatggtggg cgtcgtaccg tggatttcgc
      601 cgactgccac tgcctacccc ggcgctcgtt cacggcgatc tctggtatgg caacatcctg
      661 gtcgatccgg gacacacccg catccttggt cttcttgact gggaggagat ggcgttcgat
      721 gacccggcgc aggatttcgc caccctgcgc cattccggca acgccttcag cgatgacgcg
      781 ctcgccgcct atgcccacgc aggcggcgcg atcgacgtcg gcctcctcgc tcgccgggag
      841 tggcactggg aatgtcggga ggtcatcggg atcgcctccg ccatcaagac cggagatacg
      901 gcagagattg aggagacgct tgacaagctc atgagcgggc cggtgatgcg gtcgaggcgg
      961 cgttgatgat gtaccccttc cagcaccggc tgatctcaat ccaaggcaac ccttgtctcg
     1021 gccttccgtc gactgttaca aggtggatca ctatgaaact aaacggatgc gagccgactg
     1081 ccacgcccgt gattaccaac cctcgctgcg caagtacgcg gcaaagctca gcgtggggtt
     1141 agtgccgact tcgattaacc aggtatcggc ggcaacttct aattgagacc gagctgcgct
     1201 gtaactcgct gagtccataa tcggcgctaa agcatcgaag ccgagaaaga ttgtgaagac
     1261 ttggtcgttc ggatgagcag tcgacggcgt tggcacataa ccaccctgac ccatcagaac
     1321 ttggtagagc agatcgagat tgagattgtg gaacttcccg gacgcgccag gtgtgtagag
     1381 tacctgcatc aactcacggt aggtcgcaag gagctcatac gagttgaggt gaggtctatt
     1441 ggccacaaga ctggcaaggg cgcgaaggtg tgcgtccgca ggaagaccct gctcctttgc
     1501 tacggatgct tcgaactgag cagcgaaata gttgtccgtc ccgctggaca aaaagtgatc
     1561 ttgaagcagg cgtgacgagg tacttgtgtc gccggtaaaa cgggaaagcg atgtgccgat
     1621 cagcaggcgg gagtcgctcg gatcgatgaa cgcgccgttg ggttccggtc ctcgaccgct
     1681 ggcatcgacg agcaggcagg aatagcacgt gggctgaagc ccccgttcgg cttcgattgc
     1741 catccagttc tcccaggtcc acacattcat cggaccggaa atgctaagac gtgacgcagc
     1801 cagactgttc tccaggtctt gagtttcgcg cgagaaggaa ggatccattt gctcaagatc
     1861 gcttaggcat tcatggtcgc cgggcccaac gagtcccgtg atgtagccgc ggtacccggg
     1921 atacccagga tagagttcaa ccagttcgca acgtgacgaa cccgcgctgg attgggctga
     1981 ggacgaggga gcggtgggtg cgacagactc gacatcggca tagcgggcga tctgcgtatc
     2041 gacgaattcc cggctgcgac cgaagaacca gcgccaggaa cctgcgtcgt cctgaaccag
     2101 acgaacgaca tccgtgatcg aggtgccatc cccgaacgat tgctcgaagg ccacttcagc
     2161 cgtgtaggga tacgtttttc cggttacggg ccacacccac tccaccatct gcacgtcagt
     2221 gacggtaatc ggttgcggct gcttcgggcc gaagtcgttc tcataccact cgaccacggc
     2281 ctctcgggga atgaagacct gggcatctgg gtgcatccgg tcatatatcg cgttatagga
     2341 actgaaagct tctagcaccg agatgtcata ggcagccgtg gcggcatttt gttcatccgc
     2401 agtatcgccc tgagcaaggg cgggtgcgac acgaggaagc atcgaactta caagtgcgac
     2461 gatgaccgtc aggaatgtga gacgagacat cgcttactcc ccgaccaacg agcaacgtga
     2521 tcctctgtac agcctaattc ctgggcgctc caacggtgaa cgtctgagga caaaacacca
     2581 tggtctcggc tcccagttgc agcggtgagg ttagtcgcct ggggaagttc ccaaccttgg
     2641 cacaccgcca actacgttat gtcttggcaa catcaatata ctggaaaaat gtcagaaaac
     2701 gagtgcccta atgctgcttt cgctggtctg gcccgatcct agccgccctt ccgccaggac
     2761 gccagcaacg gcttctggtg aacagatgcg ggaaagttgg atgatcggca aactcaccac
     2821 ctgtcctcgt cagccaccat gtgcagcagc cgccacttgc ctgccctgat ttggtcggca
     2881 caactgtatg ggccggccac taccaaacgg gccatggctc tgcccgcgag agagccccgg
     2941 gggg
//
LOCUS       DASYWM010000326         4664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_412353, whole genome shotgun sequence.
ACCESSION   DASYWM010000326 DASYWM010000000
VERSION     DASYWM010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412353"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VGR29_12895"
     CDS             <1..664
                     /locus_tag="VGR29_12895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEV2074520.1"
                     /translation="IVGAIIMRAILIAFAGVLLGSFHWIIYVFGAFLIFTGYKFLRGG
                     HEAPSLESNQLVRFAKRFYPVTDGYEGQRFFVVKNGVRYMTPLFLVLPLVESTDLVFA
                     VDSIPAIYAITDDPFIVFTSNIFAILGLRALYFVLAGYLADVKYLKPGLAGVLVFVGA
                     KMLLVDVYKVPALVSLAVIVTILAVAFGASALVRRREQTATGGIGVGAPHGPDSALEK
                     TS"
     gene            1337..3202
                     /locus_tag="VGR29_12900"
     CDS             1337..3202
                     /locus_tag="VGR29_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074521.1"
                     /translation="MAGPHDRAGEHEQHSWWKVMCLTGVDYFSTLGYQPGIAVLAAGA
                     LAPLATLILVLVTLFGALPMYKRVAAASPHGDGSISMLEHLLPWWQGKFLVLCLIGFV
                     ATGFIITITLSAADATAHVVENPLTHWAHGHEVLVTLLLVSLLGAVFLKGFGEAIGIA
                     IVLVGVYLALNLVVVATGLVEIARHPDVISNWRNALTADYSNPLVMIGAALLLFPKLA
                     LGLSGFETGVVVMPLVKGDPNDTGTNVQGRIRNTRKLLTSAALIMSVMLILSSIVTTL
                     LIPAIEFEEGGEANGRALAYLAHGDLGNLFGTIYDISTISILWFAGASALAGLLNIVP
                     RYLPRYGMAPDWALATRPLVLIFTAICFVVTILFNADVDKQAGAYATGVLAVITSATV
                     AVTLAARRKHQPRATLGFGLVAAIFIYTTVVTILGDIQGLKIAAIFIVGIVVTSLVSR
                     VWRSTELRVGNVELDATAERLIADASWRGTIRIIANHPDDRSNREYLIKEREERAASH
                     IPAGDPVLFLEVNISDASEFAPSLSVRGYKVGKHAVLRTEGAAVPNTIAALLMYLRDR
                     TGKQPHIYFGWTEGNPLKYLARFILFGEGDVAPVTHEIIRRAEPDPKRRPAIHVG"
     gene            3378..>4664
                     /locus_tag="VGR29_12905"
     CDS             3378..>4664
                     /locus_tag="VGR29_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEV2074522.1"
                     /translation="MTDVLITGGRIIDGTGNAWFYGDVALSGERIERIAPPGTIDPAT
                     ATNVIDATGHVVAPGFIDIQSHSIIPFLDDRRSVSKVTQGVTTEILGEMWTPAPFGGK
                     IASPFDNALVHRLPDQAEEWEERAKGWTRFGDWLADLESRKVSVNVGSFLGGGTVREF
                     GMGNSIGEATPEAIEAMRTMTADAMEDGAFGIATALIYPPNAFSSTEELVAVMEIVAQ
                     YNGVHITHMRSEGDQIFEALAETIEIAERTGVITEIYHLKAAGIPNWPKMPKVIEEID
                     AARARGLDISADMYPYEGSGTGLAACLPPWAEADGKRQENLRDPEMRAKILEEMRNPT
                     EHWENLGARSGPDNVILAEFSKPEHKKYQGKRLSEVAEDLGMDWHEAILHLLDVEGHN
                     IFTMYLAMSEENMKLQFEQPWIKFGTDAGGIDPERVA"
BASE COUNT          918 a         1432 c         1363 g          951 t
ORIGIN      
        1 cattgtcggc gcgatcatca tgcgcgcgat cctgattgcc ttcgccggcg tgctgctcgg
       61 cagcttccac tggatcatct atgtcttcgg tgcgttcctc atcttcaccg gctacaagtt
      121 ccttcgcgga gggcatgagg ccccctcgct ggagtccaac caactggtgc ggttcgccaa
      181 gcggttctat ccggttacgg atggctacga gggacaacgg ttctttgtcg taaagaacgg
      241 cgtgcgctac atgacgccgc tcttcctcgt cctgcccttg gtcgagagta ccgacctcgt
      301 gttcgcggtc gattccatcc cggcgatcta cgcgatcact gacgatccgt tcatcgtctt
      361 cacatccaac atcttcgcca ttctcggctt gcgcgcgctc tatttcgtgc tggccgggta
      421 cctggccgac gtcaagtacc tcaagccggg actcgcaggt gtgctggtct tcgtcggtgc
      481 caagatgctg ctggtcgatg tctacaaggt gcctgcgctg gtaagtctgg ctgtcatcgt
      541 aacgatcctt gcagtcgcgt tcggcgcctc cgcgctcgtg cgccgcaggg agcagaccgc
      601 aacgggaggc ataggtgtgg gtgcgccaca tggtccggat agcgcgctgg agaagaccag
      661 ctaacgaggg gacgagggga cgcgaccgag aagctcagac tgatgttggc ccagcagacg
      721 ttgggccaac gcattcgcgg acaacatcac ggaatccata cttatacaca gcggctttcg
      781 ggagcagttg tcctgctctt gcggccggtg cgagaaatgt gggccggttt ccaggggctg
      841 gcccaaaacc accatctggc agcaggtacc aaccggggat gtcacgcgct ccttcggctt
      901 tgtacttcca gatgtttgcg caccgagcgt ccgggcgtcc tccacacgcg tgggccggtt
      961 accatgagcc ctcttttgtg gcacctgtac ccatggcaga agcgggatta atagtcgacg
     1021 accggataaa tcctgctcaa gaggacggct ccaccaacat agattccggt ggatgcaaca
     1081 cgaggccgga aaggattgcg caaccgaggc gcatggactg taaaatgcat tgagtttgct
     1141 caagatacag acgacgctcg tcgcctgaag gtaatggttg ctggagcgca attacttgaa
     1201 gactcgccct aatgtgtcca gtcgccacta cccgacacgc atgccagtcc agagccctac
     1261 gccttccaaa cggaagcgca gaggaaccac aggctccgcc gtcggtcact ggttgctcaa
     1321 ggggcaccca cgtgagatgg ctgggcccca cgaccgcgcg ggagagcacg agcagcactc
     1381 atggtggaag gtgatgtgcc tgacaggtgt cgactacttc tcgaccctgg gctatcaacc
     1441 aggcattgcc gtgctggccg caggcgctct cgccccactt gccaccctca tcctggtact
     1501 ggtcaccctc tttggcgccc tgccaatgta caagcgcgta gccgccgcga gtccgcacgg
     1561 agatggttca atctccatgc tggaacatct gctcccctgg tggcagggca agttcctcgt
     1621 tctctgcctg atcggctttg tcgccactgg cttcattatc accatcacgc tttctgccgc
     1681 cgacgccacg gctcacgtgg tggagaaccc cctcacccac tgggcccacg ggcacgaggt
     1741 tcttgtcacg ctgttgcttg ttagtcttct cggcgcagtc tttctaaagg gtttcggcga
     1801 agcgatcggt atcgcaatcg tacttgtcgg cgtctatctc gccctcaacc ttgtcgttgt
     1861 ggccaccgga ctggtcgaga tcgcccggca tcccgacgtg atttcgaatt ggcgtaatgc
     1921 gctgactgct gattacagca acccattggt gatgatcggc gcggcgctcc tccttttccc
     1981 gaagctggcc ttgggtcttt caggattcga gaccggcgtt gttgtcatgc cattggtcaa
     2041 gggagacccc aacgacaccg gcaccaatgt gcagggtcga atccggaaca cgcgaaagct
     2101 gctgacgagc gccgccctga tcatgagtgt catgcttatc ctcagcagca tcgtaaccac
     2161 cttgctcatt cccgctattg agttcgagga aggtggggaa gcgaacgggc gtgcacttgc
     2221 ctaccttgct catggagatc tcggcaatct ctttggcaca atctacgaca tcagcacgat
     2281 ttcaatcctc tggttcgcgg gggcctcagc cctggccggc ctcctgaata ttgtcccgcg
     2341 ttacctcccg cgctacggga tggcccccga ctgggccttg gccacccgac cactcgtgct
     2401 tatttttacg gccatttgct tcgtggttac gatcctcttc aatgcagacg tggacaagca
     2461 ggccggtgcc tatgccacag gagttctggc ggttataacc tccgcgacgg tggcggtcac
     2521 cctggctgcc cggcgaaaac atcagcctcg cgccacgctg ggcttcggcc tcgtggctgc
     2581 gatattcatt tacaccacag tggtaacgat tcttggggat atccagggtc tcaagatcgc
     2641 cgccatcttc atcgtaggca tcgtggtcac gtcgctcgta tcgcgtgtct ggcgatcgac
     2701 cgagttgcgc gtcggcaacg tcgagcttga tgccacagcg gagcgcctca tcgccgatgc
     2761 ctcatggcgc ggcacgatca ggatcatcgc gaatcacccg gatgaccgct ccaatcggga
     2821 gtacttgatc aaggaacgag aggaacgggc tgccagccat atcccggctg gggaccctgt
     2881 cctgttcctg gaagtcaata ttagcgatgc ctcggagttt gcgccttcac tctccgtccg
     2941 gggctataag gttggaaagc acgccgtctt gcgtacagag ggggcggccg ttcccaacac
     3001 catcgctgcg ctcctcatgt acctgcgcga tcgaacaggg aagcaaccgc acatatactt
     3061 cggatggaca gaaggaaatc cattgaagta cctcgcgcgc tttatcctgt ttggcgaagg
     3121 cgatgttgct cccgtcaccc acgagatcat tcgacgcgcc gagccagacc cgaagcgccg
     3181 cccagccatc cacgtcggct agatgctatc cgcgatgtga agcacaggcg gcacaggaaa
     3241 tccctgttca cttggtacga tctcgtgaga atcgagccat cgtccggacc tgtccgtgtc
     3301 accacgttcc catatcttct ttaaccggga tatgatggcg gcgttggaat cacctacaga
     3361 caggagagac tggattcatg acggatgtat taatcaccgg cggacggatt atcgacggca
     3421 ccggcaacgc ctggttctat ggcgatgttg ctcttagcgg cgagcggatc gagcgtatcg
     3481 caccgcccgg cacgatcgac cccgcgacag caacaaatgt catcgacgcg accgggcacg
     3541 tcgtagcgcc aggttttatc gatatccagt cccactcgat cattcccttc ctcgacgacc
     3601 ggcgctcggt atccaaagtg acgcagggcg tgacgacaga gatcctgggc gagatgtgga
     3661 cgcccgcccc cttcggcggc aagatcgctt cgcctttcga caacgcgctg gtacatcgcc
     3721 tgccggatca ggcggaggaa tgggaagagc gcgcgaaggg atggacccgc ttcggcgatt
     3781 ggctggccga cctggagtcc cgcaaggtct ccgtcaatgt cggctccttc ctcggcggtg
     3841 gaactgtacg cgagttcggc atggggaaca gcatcggcga ggcgacgcca gaggcgatcg
     3901 aggcaatgcg caccatgacc gcagacgcca tggaagacgg cgccttcggc atcgcgacgg
     3961 cccttatcta cccacccaac gccttctcca gcaccgagga gctggttgcg gtcatggaga
     4021 tcgtcgccca atataacggc gtccacatca cccacatgcg ctccgagggc gaccagatct
     4081 ttgaggccct tgcggaaacc atcgagattg ccgagcgaac cggcgtgatc acggagatct
     4141 atcatcttaa ggctgcaggc atcccgaact ggccgaagat gcccaaagtg atcgaggaga
     4201 ttgacgccgc acgggcacgt gggcttgata tcagcgccga catgtacccg tacgagggct
     4261 caggcactgg cctcgccgcg tgccttcccc cgtgggccga ggccgatgga aaacgccagg
     4321 agaacctgcg cgatccggag atgcgcgcga agatccttga ggaaatgcgt aacccaacgg
     4381 agcactggga aaacctgggc gctcggtcag ggccggacaa cgtcattctc gccgagttct
     4441 cgaaacccga gcacaagaaa taccagggca aacgcctctc cgaggtggcg gaggacctgg
     4501 gcatggactg gcatgaggcc atcctgcacc tgctcgacgt cgaggggcac aacatcttca
     4561 ccatgtacct cgcgatgagt gaggagaaca tgaagctgca gttcgagcag ccgtggatca
     4621 agttcggcac cgatgctggc ggaattgatc ccgagcgagt tgcg
//
LOCUS       DASYWM010000327         2991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_393497, whole genome shotgun sequence.
ACCESSION   DASYWM010000327 DASYWM010000000
VERSION     DASYWM010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393497"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VGR29_12910"
     CDS             <1..598
                     /locus_tag="VGR29_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEV2074523.1"
                     /translation="ARHAANLGVLWIDTHPDLNTPESSFSGHIHGMPLGTAIGLAPES
                     MRQASLLAGQVPMIQPEHLCLLGIRDIDAVEEEVIAEKGIFARTMDEWNDEGILPGLE
                     AALKHLECQGVDAIHLSFDLDVLDPAFMPGTGTRYPGGLTVREASQVLRYLGAWEGPI
                     RSMDLVELNPTLDASGYSTDIALHLLATALGERMLSRG"
     gene            complement(658..1929)
                     /locus_tag="VGR29_12915"
     CDS             complement(658..1929)
                     /locus_tag="VGR29_12915"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEV2074524.1"
                     /translation="MQERFRGNHAAGSSHGRPKAVAAVARSDDWRTAVDSLLHATLDG
                     QTTAPDLVVIFPSSAYADDYPDLIRDVWQRSGAGCLIGSSSRGVIAGSVCYESEPSIS
                     MLALWLPGATLTPVRLHQSMLDAFDDPDLQEGLYGPPAAETRGWILFADPYRMDAQDA
                     VRRLRTRYPGVPVVGALASTLKQDRQVWVFFDDQVFDEGGVALAIGGPYDLQVVVSQG
                     GDPIGEPWTITGAERNLITSISNRPAVDVMRETINALPRDVGLEVERNLMLGFPVDEY
                     QEQFHRGDFVIRGLLGIDEERGALVVGSLPRCGQTVQFQLRDAGSSALDLQQVLVDAR
                     ALVGESDVVAGMICTCKGRGSAMFGRPDHDARAVRTAFRDLPFAGMFSFGEIGPVRGV
                     PALNGFAMSLGVIVHRTENSPEPDGHPLASG"
     gene            complement(2116..>2991)
                     /gene="ispH"
                     /locus_tag="VGR29_12920"
     CDS             complement(2116..>2991)
                     /gene="ispH"
                     /locus_tag="VGR29_12920"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEV2074525.1"
                     /translation="SPESRVAPIGDIIHNPQVVDRLRERGVEGATSVEVAKDRGFRRV
                     AITAHGMGPHLAVEADTNGMELIDTTCPLVTKVQRLAQKLVRQGYYLVVYGDSYHPEV
                     KSVLGWANTSKSCAAKHAEDLPWNAARGEKGEGKVSPPRKVALVSQTTKNIDELMVFS
                     NQLQRMVMPEGGEFRLCNTICEPTTERQNALKRLTDQVHLVFAIGGKKSSNTARLAEV
                     GNQMGVQSYHIERADDIQDEWLTAVGDGNIGITAGASTPDDVIEDVVAYLAERGFNPP
                     EGGIRPVDPDYVPAF"
BASE COUNT          583 a          944 c          906 g          558 t
ORIGIN      
        1 cgcccgtcat gcagcgaatc tgggtgtgct ctggatcgat acccatccgg atctcaatac
       61 ccccgagagc agcttcagcg ggcatatcca cggcatgcca cttggcaccg cgatcggcct
      121 agcaccggag agcatgcggc aggcgtctct tctcgccggc caggtaccga tgattcagcc
      181 ggagcatctg tgcctgctcg gcattcggga tatcgatgcg gtagaagaag aggtcattgc
      241 cgagaagggt atcttcgccc ggacaatgga cgagtggaac gacgagggga ttctccccgg
      301 ccttgaggca gccttgaaac atctggagtg ccagggcgtc gacgcgattc acctttcgtt
      361 cgatctcgat gtgcttgatc ccgcctttat gccgggcacc ggtacgcgct accctggcgg
      421 gctgactgtc cgggaggcat cgcaggtact gcgctatctc ggtgcctggg aaggaccaat
      481 ccgctcaatg gatttggtcg agctcaaccc aacactcgat gcctcgggct acagcacgga
      541 tatcgcgctc caccttcttg ccaccgccct tggcgagcgc atgctgagcc gcgggtagcc
      601 ttccgcagcc caaagttcct ccctgaaccg gtgtaggggc tgacttccgt aggaatctca
      661 gcccgatgcc aggggatgac catcaggttc cggggagttt tcagtccgat gcacgatcac
      721 ccccagcgac atcgcgaacc cgttgagggc cggcacgcca cgtacgggac cgatctcgcc
      781 aaaggagaac atccccgcga aagggaggtc ccggaacgct gtccgaacgg cacgcgcgtc
      841 gtgatccggg cgaccgaaca tggcgctgcc gcgaccttta caggtgcaaa tcatcccggc
      901 aaccacgtcg ctttcgccga ccagcgcgcg ggcatccacg agcacctgct gcaggtccag
      961 ggcagagctc ccggcatcgc gtagctggaa ctgcaccgtc tgcccacaac gagggagact
     1021 gcccacgacc agggcgccac gttcctcatc gatgccaagc agtccccgga tgacgaaatc
     1081 accccggtgg aactgttcct gatactcgtc taccgggaag ccgagcatca ggttgcgctc
     1141 gacctccagg ccgacgtccc tgggcaaggc attgatcgtc tcgcgcatca catcgacagc
     1201 gggacgattg gagatcgatg tgatgaggtt ccgctcggca ccggtaatgg tccatggctc
     1261 gccgatcggg tcccctccct gcgacaccac gacctgaagg tcatagggcc cgccgatcgc
     1321 aagcgcgaca cctccttcat cgaacacctg gtcgtcgaag aagacccaaa cctggcgatc
     1381 ctgcttcagg gtcgatgcga gagcaccgac gactggaaca ccgggatacc gggtgcggag
     1441 gcggcgcacg gcgtcctggg cgtccattct gtacgggtca gcaaagagga tccagccacg
     1501 cgtttcagcc gcgggtgggc catagagacc ctcctgcaga tcggggtcgt cgaatgcatc
     1561 cagcatcgat tgatgcaacc gcacgggtgt cagtgttgcc ccgggtagcc agagtgccag
     1621 catcgagatc gatggctcgc tctcataaca gactgatccg gcaatgactc cacgagacga
     1681 gctcccgatc aggcaacctg cccccgaacg ctgccagacg tcacgaatga gatcagggta
     1741 gtcatcggcg taggcgctgc tggggaagat caccacgagg tccggcgcgg tggtttggcc
     1801 gtcgagcgtt gcgtgcagca gggagtcgac cgcggtgcgc caatcatcac ttcgcgctac
     1861 ggctgccacg gcctttgggc gtccatgcga ggagcctgcc gcatgattgc ctctgaaacg
     1921 ctcctgcatt cgcactctcc ataggtgccc ttgaattgaa tgatgtcggt gagccgatgg
     1981 aaaaaccgat catacccgcc tttgccacag acgcaagagg caaaccggat acagctgcac
     2041 gaggagacgc caacaagaaa gcggcgatcc cggaatccag gatcgccact ctttcgtttt
     2101 tcgcttaatc agggatcaga atgccggaac gtagtccggg tcgacggggc ggataccgcc
     2161 ctctggcggg ttgaatccgc gctcggcgag ataggcaacg acgtcttcga tcacgtcgtc
     2221 cggggtggaa gcacccgcgg taatgccgat attgccatca cccacggcgg tgagccactc
     2281 gtcctggata tcgtcggccc gctcaatgtg ataggactgc acgcccatct gattgccgac
     2341 ctcggcgagc cgtgcggtat tcgagctctt cttgccaccg atggcgaaga ccagatgcac
     2401 ctggtcggtc aatcgcttga gggcattctg gcgctcggtg gtcggctcac agatcgtgtt
     2461 acagaggcgg aattcgccgc cctctggcat caccatccgc tgcaactgat tggagaagac
     2521 catcagctca tcgatattct tggtcgtctg gctcaccagg gcgaccttgc gcggtggcga
     2581 taccttgccc tcacccttct cgccccgcgc ggcgttccag ggcaggtcct ccgcgtgctt
     2641 ggcggcacat gacttggatg tgttcgccca accgagcacg ctctttactt cgggatgata
     2701 tgagtcgccg tagaccacga ggtaatagcc ttgacggacg agcttctgtg ccaggcgctg
     2761 gaccttggtt accagcgggc aggtggtatc gataagctcc atgccattgg tgtccgcctc
     2821 gacggcgaga tgagggccca taccgtgggc agtgatggca acgcggcgga aaccgcgatc
     2881 tttcgccacc tccacggagg tcgccccttc aacgccgcgc tcgcgaaggc gatccacaac
     2941 ctgcgggttg tggatgatat cgccgattgg tgccacgcgg ctttctggcg a
//
LOCUS       DASYWM010000328         4512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_618950, whole genome shotgun sequence.
ACCESSION   DASYWM010000328 DASYWM010000000
VERSION     DASYWM010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618950"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VGR29_12925"
     CDS             complement(<1..864)
                     /locus_tag="VGR29_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEV2074526.1"
                     /translation="MTRSVVLGVDSSTQSTKVLALDVETGEVVGSGRAPHSGADIQSP
                     EEWWQALALAVRQVLSSGIEVKAISVAGQQHGCVLIDAKGEVVRPAPLWNNVASAPDA
                     EQLNAMADFSAEVGSLLVASFTITKLAHISRTVKEDISRTAAVGLPHDWLNFRLTGEL
                     TTDRGDASGTGWWSPRDGEYRRDLLALAVGEDDAGRINLPILRGPEEPASLLSKLAAN
                     ALGLPAGIPVGPGTGDNMAAALGVGAKPGEIIISLGTSGTAFAVSETPTSDPTGEVAG
                     FADATGRFLPLA"
     gene            complement(1003..2178)
                     /gene="xylA"
                     /locus_tag="VGR29_12930"
     CDS             complement(1003..2178)
                     /gene="xylA"
                     /locus_tag="VGR29_12930"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEV2074527.1"
                     /translation="MSDTYAPQKSDRFTFGLWTVGNMGRDPFGDPVRNAVDPVHSVQK
                     LAELGAWGVNFHDNDLVPFDASPHDRDRIVREFKQALDDNGMVVPMATTNLFTHPIFR
                     DGAFTSSDKLVRAFALQKTMAAIDLGAEVGASTYVFWGGREGSETEAYRDAADALKWF
                     REAVNYLSEYVRDQGYGMRFALEPKPNEPRGDLYLPTVGHALHFISTLDHPEMVGVNP
                     EFAHETMAGLNFVHGVAQALEANKLFHIDLNDQQPGRYDQDFRFASENPKRAFFLIKL
                     LEDSGYDGPRHFDAHAYRTEDQDGVWAFARGCMRSYLILKEKVQQFHADPEISRVIAE
                     LKEMDNAYDGPEASAGYTGDHAEALRQQAFDTETMAQRGRRYEELDQLVMDLLLGAR"
     gene            complement(2309..3481)
                     /locus_tag="VGR29_12935"
     CDS             complement(2309..3481)
                     /locus_tag="VGR29_12935"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2074528.1"
                     /translation="MAEIGVGMVGYKFMGRTHSNAYRQVARFFDVDPEPRMVALAGRD
                     EASVKEAAEDLGWESYVTDYHGLIERDDIQLVDVASPGFTHKEIVVAALEAGKHVLCE
                     KPLSNTLDEAQEMLSAARKAGTIAMVNFNYRRVPAVQLAKRLIDDGRIGEIRHFRANY
                     LQDWILDPQFPLVWRMKKDLAGSGALGDIGAHIVDLGQYLVGQITEVVGMMETFVKQR
                     PLEVSSGGGSGLSASAGQEMGDVTVDDASSFLAKFENGAMGSFNATRFAAGRRNHNTF
                     EINGSLGSISFNLERMNELEVFLVEDEEGLQGFRTINVTESVHPYTGNWWPAGHIIGY
                     EHTFVHVVKDLIDGISKGVTPHPDFEDGYRNQAILDAVERSAESRSWTSPKEPRLS"
     gene            complement(3538..>4512)
                     /locus_tag="VGR29_12940"
     CDS             complement(3538..>4512)
                     /locus_tag="VGR29_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV2074529.1"
                     /translation="GQWADLPLEELAPKAKEMGFDGLELACWGDHFDVQRAITEPNYC
                     ESRWDILNKNDLKCFAVGNHLVGQAVCDAVIDQRHEGILPPHVWGDGEPESVRQRAAE
                     EMKDTARAAARLGVPVVTGFTGSAIWHVLAMFPPVPPEMIEAGYQDFADRWNPILDVF
                     DEVGVKFALEVHPSEIAYDYWTTKRTLEAIDHREGFGINFDPSHLYWQMLDPVAFVHE
                     FGDRIYHAHVKESIRVLDGRNGILGSHLLFGDHRRGWDFVSPGRGGVPFERVFRALND
                     VGYDGPLSIEWEDNGMNRNQGAPEAVELVKRLDLVPSDVAFDAAFQQN"
BASE COUNT          900 a         1490 c         1255 g          867 t
ORIGIN      
        1 cgccaggggc aagaaccgac ccgtggcgtc tgcgaacccc gcaacctccc cggtcgggtc
       61 ggatgtcggc gtctcgctca cggcgaatgc agtgccgctc gttcccaggc tgatgatgat
      121 ttcgcctggc ttggcgccca cccccaatgc agccgccata ttgtccccgg tgcctggccc
      181 gacagggata cctgccggca gcccaagcgc atttgccgcg agcttggaga gcaggcttgc
      241 cggctcttcg ggcccacgca ggatcggcag gttgatccgc ccggcatcat cctcaccgac
      301 cgcaagggcc aggagatccc gtcgatactc gccatcgcgt ggcgaccacc agccggtacc
      361 cgaggcgtcc ccccggtccg ttgtcagctc gccggtgaga cggaaattca accagtcatg
      421 tggcaaaccg acggctgcag tgcgagagat gtcttccttc acggttcggg agatatgggc
      481 gagcttggtg atcgtaaatg acgcaaccag caaggacccg acctctgctg aaaaatcggc
      541 catcgcgttc agctgctcgg catccggagc cgaagcgaca ttattccaaa gcggcgctgg
      601 acgaaccacc tcgcccttcg catcgatgag gacgcacccg tgctgttgcc cggcgacgga
      661 gattgccttg acttcgattc ccgatgacag aacctggcgg acagcgagcg cgagcgcttg
      721 ccaccactct tcaggagact ggatgtccgc cccgctatgt ggtgcccgcc ctgacccgac
      781 aacctccccc gtctcgacat ccagggcaag caccttggtg gactgcgtgc tcgaatcaac
      841 tcccagaacg acagaacggg tcatacagtt tcctttgctt gagctagtgc aggagccact
      901 actctttgcc gttcaacacg ccggaaagta gctcgtcggc attgccaggg cagatcatgg
      961 agcccgatga ttccgatccg ccccctattc tcaatcgaga tcctagcggg ctccgagcag
     1021 gagatccatc acgagctggt ccagctcttc gtatcgacga ccgcgctgcg ccatcgtctc
     1081 cgtatcaaac gcctgctgcc tgagtgcctc ggcgtggtca ccggtgtaac cagcgctcgc
     1141 ctcggggcca tcgtacgcgt tgtccatctc cttaagctcg gcaatcacgc ggctgatctc
     1201 cgggtcggca tggaactgct gcaccttctc cttcaggatc aagtaggatc gcatgcaccc
     1261 gcgggcgaag gcccaaacgc cgtcctgatc ctcggtccgg taggcgtgcg cgtcgaaatg
     1321 acgcggacca tcatagccgc tatcctcgag caacttgatg aggaagaagg cacgcttcgg
     1381 attctccgag gcgaaccgga agtcctggtc gtaccggcct ggctgctggt cattgaggtc
     1441 aatgtggaag agcttgttcg cttcgagcgc ctgtgccact ccatggacga agttcagccc
     1501 tgccatggtc tcgtgagcaa actcggggtt cacacccacc atctccgggt gatcgagcgt
     1561 cgagataaag tggagtgcgt ggccaaccgt tgggagatac agatccccac gaggctcgtt
     1621 gggcttcggc tccaaggcga accgcatgcc gtatccctga tcgcgaacgt attcactcag
     1681 gtagttgact gcctcacgga accacttcag tgcatccgcg gcgtcccggt aggcctcggt
     1741 ctccgaacct tcacgtccgc cccagaagac gtaggtcgac gctcccacct cggcaccaag
     1801 atcgatcgcc gccatggtct tttgcagcgc gaaggcacgt accagcttgt cggaagaggt
     1861 aaaagcccca tccctgaaaa tgggatgcgt gaacaggttc gttgtcgcca tcggcaccac
     1921 catgccgttg tcatcaagcg cctgcttgaa ctcacgaacg atgcggtccc gatcatgggg
     1981 agaggcatca aagggaacca gatcattgtc gtggaaattg actccccacg ctcccagctc
     2041 cgcgagtttc tgcacgctat ggaccggatc gaccgcgttc cgtaccggat ccccgaatgg
     2101 gtcgcgaccc atgttaccga ccgtccataa gccgaaggtg aacctgtcgc tcttctgtgg
     2161 agcgtatgta tccgacattt gccctctcct cccttgtcca gatgaaccgg tcgccccttg
     2221 ggcgtcaggt gccaaggcac caccacggcg ctaaccccgt tgaaatgctg gtgagccggt
     2281 caaaagcacg tccgcagtgc ggcccacgct agctcaaccg cggctccttc ggtgatgtcc
     2341 agctccggga ctccgcgctc cgctccacgg catcaaggat ggcctggtta cggtacccgt
     2401 cctcgaagtc ggggtgcgga gtcacgccct tgctgatgcc gtctatcagg tccttcacaa
     2461 cgtgcacgaa ggtgtgctca taaccgatga tgtgcccggc aggccaccag ttgccagtgt
     2521 aagggtggac agactccgtc acattaattg tccggaagcc ctgcagcccc tcctcatcct
     2581 cgaccaggaa tacctcaagc tcgttcatgc gctcaagatt gaaggaaatc gagccaaggg
     2641 agccattgat ctcaaaggtg ttgtggtttc gccggcctgc cgcaaagcgg gtggcattga
     2701 agctccccat cgcgccgttc tcgaatttcg ccaggaagct cgaggcgtca tcgaccgtca
     2761 catcgcccat ctcctgcccg gcacttgccg acaggccaga tccgccgccg ctgcttactt
     2821 caagcggacg ctgcttgacg aaggtctcca tcatgccaac gacttcggtg atctggccca
     2881 cgaggtattg acccaggtca acgatgtggg cgccgatatc cccaagcgca ccggagcctg
     2941 ccagatcctt cttcattcgc cagacgagcg gaaactgggg atccagaatc caatcctgga
     3001 gataattggc acggaaatgc cggatctccc cgatccggcc atcgtcgatc aaccgcttgg
     3061 ccagctggac tgccgggacg cgccggtagt tgaaattgac catggcgatc gtgccggcct
     3121 tgcgggccgc ggaaagcatc tcctgcgctt cgtcgagcgt gttggacaac ggcttctcac
     3181 acaagacatg cttgcccgcc tccaatgccg caaccacaat ctccttgtgg gtaaaacccg
     3241 gcgacgcaac atccacgagc tggatatcgt cgcgctcgat caggccatga tagtccgtga
     3301 cgtaggactc ccagcccaga tcctcggcgg cttccttgac tgacgcctca tcccgccccg
     3361 ccaatgcaac catccgtggc tccggatcga catcaaagaa cctcgccacc tggcgatagg
     3421 cgttcgaatg ggtgcggccc ataaacttgt agcccaccat gccgacaccg atctctgcca
     3481 tagttcatct cctagcaaaa acgaccgaac tccgccgctc ggaatcctta tgacacatca
     3541 attctgctgg aatgccgcgt cgaacgcgac atccgatggc acgaggtcta gtcgcttcac
     3601 caactccacc gcttccggtg cgccctgatt ccggttcatg ccgttatcct cccactcgat
     3661 agacagtggg ccatcgtaac cgacatcgtt cagcgcccgg aacacgcgct caaatggaac
     3721 accaccacgg cccggtgaca caaaatccca tccccgacgg tgatcaccaa acagaaggtg
     3781 ggatccaaga atgccgttgc ggccatcgag cacccggatc gattccttaa catgcgcatg
     3841 gtagatccga tcgccgaact catggacgaa cgcaaccggg tcgagcatct gccagtagag
     3901 gtgactgggg tcgaagttga taccgaaccc ctcgcggtga tcgatcgctt ccaaggtgcg
     3961 cttcgttgtc caatagtcat aggcgatctc ggacgggtgc acctcaagcg cgaacttgac
     4021 cccgacctcg tcgaagacat ccaggatggg gttccaccta tcggcgaagt cctggtagcc
     4081 ggcctcgatc atttcggggg ggaccggcgg aaacatcgcg aggacgtgcc agatggcgga
     4141 cccggtgaac ccggtcacga ccgggacgcc taaccgcgcc gcggcccggg ccgtgtcctt
     4201 catttcctct gctgctcgct gccgcacgct ctcgggttcg ccatcacccc agacatgcgg
     4261 gggcaggata ccctcgtggc gctggtcgat gacggcgtca cagaccgcct ggcccaccag
     4321 gtggttgccg acggcgaagc acttcaggtc gttcttattg agaatgtccc agcggctctc
     4381 acaataattc ggctccgtga tcgctcgctg gacatcgaag tgatcacccc aacacgcgag
     4441 ctccaggccg tcgaagccca tctccttcgc tttgggcgca agctcctcga gcggcaaatc
     4501 ggcccattgg cc
//
LOCUS       DASYWM010000329         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_168779, whole genome shotgun sequence.
ACCESSION   DASYWM010000329 DASYWM010000000
VERSION     DASYWM010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168779"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VGR29_12945"
     CDS             complement(<1..494)
                     /locus_tag="VGR29_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV2074530.1"
                     /translation="MSSEVQDVEHMDIALDPEDAREYELARMRHSAAHLMAEAVQEIF
                     PDARFGIGPAIDNGFYYDFDLPRPLTPEDLKEIEKLMKKNQKRNESFERNVVSREEAM
                     EIFKNNPYKLELIQNFPDDEEISTYQQGNFLDLCRGPHVESTRKIGPFKLQSVAGAYW
                     RGDE"
     gene            complement(813..1994)
                     /locus_tag="VGR29_12950"
     CDS             complement(813..1994)
                     /locus_tag="VGR29_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2074531.1"
                     /translation="MTLLRLQALIILSLLVSLPLPAGSSVAQEVPELQITSSHYILLD
                     AETGEVYAQRNADKEVAIASLTKVFTAVQALNMAPLDTPITTKSSDLQSAEATTMGFG
                     PGETYTLEDLIYGMLLPSGNDAAYAIARSLGYQKGDTDKQAVDRFMALVNQRVENMGL
                     KHTHLLNPDGWGVPGHYSSASDVAAFMRYAIEYPFLVDVMGTRSYTTSNGLLTVTNSN
                     KLLNSYGALVAGKTGYDDDAGWCLVTLAESRNTRMVAVTLDGVAPDDWYDDNRVLLDY
                     GFRRKPEIAASGDRFNGDIVGFVDPDLAQLAKSGKPEAAISGSAAVTAQFDPPRPSGN
                     ILPANGRSTDMNPTRATTWLAFIAAFALVGASGALHWRGSGDPISRTERARVDFRRSR
                     S"
     gene            complement(2117..2190)
                     /locus_tag="VGR29_12955"
     tRNA            complement(2117..2190)
                     /locus_tag="VGR29_12955"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2154..2156),aa:Val,seq:gac)
BASE COUNT          456 a          633 c          688 g          462 t
ORIGIN      
        1 ttctcgtcac cccgccagta ggcgccggcg acggactgga gcttgaacgg cccaatcttg
       61 cgcgtgctct ccacgtgcgg gccgcggcag agatcgagga agtttccctg ctggtatgtc
      121 gatatctcct catcgtcggg aaagttttga atgagctcga gcttgtaggg attgttcttg
      181 aagatctcca tcgcttcctc acgggaaacg acattgcgct caaaactctc gttgcgcttc
      241 tgattcttct tcatcagctt ctcgatctcc ttcaggtcct ccggcgtgag agggcggggg
      301 agatcgaaat cgtagtagaa gccgttgtct atggccggtc cgatcccgaa cctggcgtcg
      361 gggaaaatct cctgcaccgc ttcagccatc aagtgggcgg cggaatggcg catgcgggcg
      421 agctcgtact ccctcgcgtc ctcggggtcg agggcgatat ccatatgttc gacgtcttgt
      481 acttcgctgg acatgatgtt cctcctgtgg agaatacggg acacaaaacg gcccggcagt
      541 ccgcaaggga cgccgggccg acgtggttcc acccttcttc cgaacgctga ccaggaggat
      601 ggcgcagcat tcgctcgatt tccccggtaa cgggaggacc cgggcagcga tagccgaggg
      661 aaaacctccg ggttcacgtg cctgctcaca ggtggtatcg cgaccgctaa cgatggtccg
      721 gcttacacca ctcccggatt cgctgtcatg tcccgccgcg cgacatgtcc tgatcaatgc
      781 atgagtatct taggttgtac ctgcaaacaa gcttaggaac gagagcgcct aaagtcaacc
      841 cgtgctcgtt ccgtgcgtga gatcggatcg cccgaaccgc gccagtgtag tgcgccgctc
      901 gcgccgacca gcgcaaatgc tgcaataaat gcgagccacg tggttgcccg agtgggattc
      961 atatctgtgg agcggccatt cgcaggcaag atgttacccg acggccgggg cggatcgaac
     1021 tgcgcggtga cggcagcaga accggagatc gccgcctccg gcttgccgct tttcgccagt
     1081 tgagccagat ccgggtcgac gaatcccaca atatccccat tgaaccggtc acctgacgcg
     1141 gcgatctccg gcttcctgcg aaagccatag tcgagcagga cgcgattgtc atcgtaccag
     1201 tcatctggcg ccactccatc aagcgtgacg gctaccatcc gtgtattgcg tgactcggca
     1261 agggtgacca ggcaccaacc agcgtcgtcg tcatagccgg ttttgcctgc caccagcgcg
     1321 ccatagctgt tcagcaattt attggagttg gtgacggtaa gaagcccgtt ggacgtggtg
     1381 taggagcgag tccccatgac atcgacgaga aagggatatt caatagcgta acgcatgaag
     1441 gccgccacat cggaggcaga ggaatagtga cccggaacgc cccatccatc cgggtttaga
     1501 aggtgggtgt gcttcaggcc catgttttct acacgctggt ttaccagggc catgaaccgg
     1561 tctaccgctt gtttgtcggt gtcacccttc tgatagccca ggcttcgcgc gatcgcgtag
     1621 gcagcatcgt tgcccgacgg gaggagcatg ccgtagataa gatcctcgag cgtatacgtc
     1681 tcgcccggtc caaagcccat cgtcgtcgcc tcggcactct ggagatcgga cgattttgtg
     1741 gtgatcggtg tatcgagggg agccatgttg agggcctgga ccgcagtaaa gaccttggtc
     1801 aggctcgcga tggccacttc cttgtcggca ttgcgttggg cgtagacttc gccagtctca
     1861 gcgtccagca gaatataatg ggaagacgtg atctggagct cgggaacctc ttgcgccacg
     1921 ctgctgcccg cggggagcgg caaggatacg aggagagaga ggatgatgag agcttgcagc
     1981 cggagaaggg tcatggagcc accctgggat gtctgtggag gaaatcgggg gtgggaaact
     2041 gcggtccgtg agggtctcat attcatgggg aaatgataaa caaaaagcca gccgcgttga
     2101 ggtggctggc cttgggtggg cgatactgga ctcgaaccag tgacctccac gatgtcaacg
     2161 tggcgctcta accaactgag ctaatcgccc ttcagcacgc gaagtatagg ggccacgaca
     2221 acgcggtgca agggcttcc
//
LOCUS       DASYWM010000330         6130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_412709, whole genome shotgun sequence.
ACCESSION   DASYWM010000330 DASYWM010000000
VERSION     DASYWM010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412709"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..825)
                     /locus_tag="VGR29_12960"
     CDS             complement(43..825)
                     /locus_tag="VGR29_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2074532.1"
                     /translation="MTRLTVLGGSAAGVGTGQGCSGYLVQSDSTNLVLDLGPDTLLEL
                     RKHANFRTLDGIVISHLHLDHVLDLFALRFALKYNPEPAPKAIPLHLPPDGLAFMERA
                     AALFVTPGEDTDDYFSSVFDLSEYDPGSEVTVGDFTLTIAPTAHVIPCWAIRVHSADN
                     ADDLFYTADTGTDADLDPIAAGAAVMIAEAAMPPDSDPQKIGSLHLTPQQAGDTAERA
                     GARELVLTHIWEELGPDTSAEAARKHFSGRVTIATPGASFSW"
     gene            complement(877..1308)
                     /locus_tag="VGR29_12965"
     CDS             complement(877..1308)
                     /locus_tag="VGR29_12965"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2074533.1"
                     /translation="MPGLSEIDFGQIEGLTIHQVLEQFPELQPHLDDLHDMELTSPGG
                     ESRRGFVERVMATFLGLIDQYEGQKIVVVCHGGVISSFYAQLEQGPVNDFVRYAVANC
                     SVSHFIVTPEQTLIQMWNDIKHLDEVQEGPVKLIPSLMEGR"
     gene            complement(1319..1612)
                     /locus_tag="VGR29_12970"
     CDS             complement(1319..1612)
                     /locus_tag="VGR29_12970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2074534.1"
                     /translation="MTTTTNIASHSDRSTASDLVNHPREGTTELFLVRHGQTPANVNH
                     LLVGSTDIPLDALGERQARMVGARFAGIDIDAVVTSPLQRAQDSGGDRRGHGA"
     gene            complement(1581..2282)
                     /gene="nth"
                     /locus_tag="VGR29_12975"
     CDS             complement(1581..2282)
                     /gene="nth"
                     /locus_tag="VGR29_12975"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV2074535.1"
                     /translation="MQQPPIPIPEEIIDKFENIERLLDVTYGPRVLRASRDPISELIG
                     TILSQNTSDTNTSRSMATLRERFPTWQHVRDAATVDIVDAIRSGGLANRKAPRIQAVL
                     EEVVARQGDTDLTFLAGMPLEEAKAWLTSMNGIGPKTAACVLLFSLGRPAMPVDTHVG
                     RVMARLGVVPDRTSTITKERILETLIGPDPDTVYAIHVETIEHGRRICRAQRPKCPEC
                     PLREYCDYYNEHCLA"
     gene            2484..3812
                     /locus_tag="VGR29_12980"
     CDS             2484..3812
                     /locus_tag="VGR29_12980"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV2074536.1"
                     /translation="MPTRAIVIRGGRVVDPVSGRDEVADIRIEDGVVTSVGHVDAPGD
                     AEVIDAEGLLVSPGWIDVHVHLRQPGFDAKESIATGTAAAVAGGFVAVACMPNTKPAL
                     DSPEVLRTLLEDVDRTGVARVYPIATITRGRTGDEAVDFDAVIDAGAIGWSDDGDTTA
                     NSAIMREVLVASRRLNRPVIVHCEDKAIATGAMHEGEVSRKLGIEGIPAAAEEIIIAR
                     DLMLAELTGGWLHVCHVSTGRGIELIHEAKQRGVNVTAEVMPHHLVMSDEWVAGSREL
                     HNTDEAAGAQAQPCDPNTKVNPPLRSQDNTRKLLKALQDGWFDCFGTDHAPHADPEKS
                     VEYQQAAFGLSGLEFAFPLSYALVRAGHLGVADLVRRWTVEPAKILGVDPMSIREGSV
                     ANITVFDPEESWTVTPEALKTKSKNTPLLSMTLRGRAVLTIVDGEVQHRA"
     gene            3805..4953
                     /gene="carA"
                     /locus_tag="VGR29_12985"
     CDS             3805..4953
                     /gene="carA"
                     /locus_tag="VGR29_12985"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV2074537.1"
                     /translation="MPDAKTRYAPEWDAVLALADGKVFRGKGFGAGIDGSGEVVFTTS
                     MTGYQEVCTDPSFRGQIVCMTYPLIGNYGVTQEDNQSRRPWIAGLVVHEVAPQPSNWR
                     SEGTLDDYLRRYNVPGISGVDTRALTRHIRSVGDMRAVLVHNAKDMTEEEIVARAQAA
                     KLPGERDVVGDVMNAEEATFGDENAGPHVAVVDCGVKMNIINSLTSRGARVTVLPYGT
                     SYDRVRELDPDGVIVSPGPGDPANLDEGLGIVRSLLEDRRPYFGICLGHQLLARAIGA
                     ETGKLKFGHRGGNQPIMDDRSGRVTMTAQNHSFQVNAGSVPTGDGWQVALRNLNDGSV
                     EGLAHRDLPVLSVQFHPEAAPGPADNGVLFDEFLEMISVPQQDHRGKN"
     gene            4955..>6130
                     /gene="carB"
                     /locus_tag="VGR29_12990"
     CDS             4955..>6130
                     /gene="carB"
                     /locus_tag="VGR29_12990"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV2074538.1"
                     /translation="MTAGSQEHVSRRPESVLVIGSGPIVIGQAAEFDYAGTQACQSLR
                     EEGIRTILVNSNPATIMTDEDIADAVYIEPLTPDVIRRVIQRERPEGLLPTLGGQTGL
                     NLAVQVSELGILDRYNVRLLGTPLSAIQKAEDRELFKDLLNEIGEPAIESEVIHSLEE
                     AREFVKRVPLPLVIRPAYTLGGTGGGVAYTDQDLERIVASGLRASPIRQVLLERSLMG
                     WKEIEYEVMRDSADTCITICNMENFDPMGVHTGDSIVVAPSQTLSDREYQMLRTSALN
                     IIRALGIEGGCNVQYALNPHSMEYGVIEVNPRVSRSSALASKATGYPIARISTKIAIG
                     KRLDELTNVVTGKTTAAFEPALDYVVAKIPRWPFDKFPRADRSITTQMKATGEVMAID
                     "
BASE COUNT         1218 a         1743 c         1956 g         1213 t
ORIGIN      
        1 caagagagac gccgctgaca tccctgatcg tcgtcgtggc ctctaccacg agaaggaagc
       61 accgggtgtg gcaatagtca cacggccgga gaagtgtttt ctggcggcct ctgcggacgt
      121 atccggcccc agttcttccc aaatatgcgt aaggaccagc tcgcgtgcgc ctgccctctc
      181 agcagtgtca cctgcctgct ggggagtgag atggagactg ccaattttct gcgggtcgga
      241 gtcaggtggc atggcggctt ccgcgatcat gacggccgcg cccgctgcga ttgggtccag
      301 atccgcatcg gtgccggtgt ccgccgtgta gaagagatcg tcggcgttgt cggcagagtg
      361 gacgcggatt gcccagcagg ggatgacatg agccgttggc gcgatggtaa gcgtgaagtc
      421 tccaacggtg acctcggagc ccgggtcgta ttcgctcaga tcaaacaccg aggagaagta
      481 gtcgtcagtg tcctcgcctg gagtgacgaa aagggccgca gccctctcca taaaggcgag
      541 cccgtcggga ggtaggtgga gtgggatcgc cttcggggct ggctcggggt tgtacttgag
      601 ggcaaagcga agcgcgaaca aatcaagcac gtgatccaga tgcaggtgag aaatcacgat
      661 cccgtcaagt gtgcggaaat tcgcgtgctt gcggagctcc agcaatgtat ccgggccaag
      721 atcaagcacc aggtttgtgc tgtcactctg gacgagatat ccggaacagc cctgtccggt
      781 gccaacgccc gcggcactcc cgccaagcac ggtaagtcgg gtcatccttc gaactccttg
      841 attcccacga atacggctgt caggctaaga acgatgtcat cgaccttcca tcagcgatgg
      901 tatcagcttc accggtcctt cctgtacctc atcaaggtgt ttgatgtcgt tccacatctg
      961 gatgagcgtc tgctcgggcg tgacgataaa gtggctgacg ctgcagttgg cgacagcgta
     1021 ccggacaaaa tcgttgaccg gtccttgttc cagctgggcg tagaacgagc taatgacgcc
     1081 tccatggcag acgactacta tcttctggcc ctcgtactga tcgatcagtc ccaggaacgt
     1141 cgccataacg cgctccacga agccacggcg tgattcgcct cccggcgagg tgagttccat
     1201 atcatgtaga tcgtcaaggt ggggttggag ttccgggaat tgctccagga cctggtgaat
     1261 ggtgagcccc tcgatctggc cgaagtcgat ctcgctgagg cctggcacaa ccagaggctc
     1321 atgccccgtg acctcggcga tcgcctccgc tgtcctgcgc gcgctgaagc gggctcgtca
     1381 ccacggcgtc aatgtctatc ccggcgaaac gggcgcccac cattcgggcc tggcgctcgc
     1441 cgagcgcatc gagcggtatg tcagttgagc caacgagtag atgattgaca ttcgcgggcg
     1501 tctgaccgtg ccgtaccagg aacaactccg tcgtcccttc ccgcggatga ttgaccagat
     1561 cggaggccgt tgatcgatcg ctatgcgagg caatgttcgt tgtagtagtc acagtactcc
     1621 ctgagaggac attcggggca ttttggccgt tgcgctcggc aaatccggcg accgtgctcg
     1681 atggtttcaa cgtgaatggc atagacggtg tccggatcag gaccaatcag tgtctcgagg
     1741 atgcgctctt tggtgatcgt actcgtccgg tctggaacga cgccgagtcg agccatgacc
     1801 cgacccacat gggtatcgac tggcatggcc ggtcttccaa ggctgaagag cagcacgcac
     1861 gccgcggttt tcggtccgat gccattcatg ctcgtcagcc atgccttcgc ctcttcaagc
     1921 ggcatcccgg caagaaaggt aagatcggta tctccttgcc tggcaacgac ttcttccagc
     1981 accgcctgga tacggggcgc cttccggttg gcaagaccgc cggaacgaat tgcgtcgaca
     2041 atatccaccg tcgctgcgtc tcggacatgt tgccatgtcg ggaagcgctc gcggagtgtc
     2101 gccattgaac gcgacgtatt ggtgtcagaa gtgttctggg acagaattgt tccaataagc
     2161 tccgagattg ggtcgcggct ggcacgcaga acacgcggtc cgtatgtgac atcgagaagt
     2221 cgctcgatat tctcgaactt gtcgatgatc tcctcgggga tggggatcgg gggttgctgc
     2281 atacgttttc ctgtcgggtg ccaagcgccg gtggtatact ctcgtcgttt gcgtggtatg
     2341 cgagaggcta ctcctgaaca tgatcttccg tctccccaac accataaccc agatctcgat
     2401 gccctcgggt cctgaaacgg acgtcggggg tgttttttta tgatgtgctg tatagcagat
     2461 ggccaagacg gagaggcggg accgtgccaa cgcgagcgat agtcattaga ggcggacgag
     2521 tggtcgatcc cgtgtccggt agggacgagg tggccgatat ccgcatcgag gatggcgtag
     2581 tcaccagtgt cggacatgtt gacgctccag gggatgcgga ggtcatcgat gcggaaggcc
     2641 tgcttgtcag tccaggttgg atcgatgtgc acgttcatct tcgccaacca ggtttcgacg
     2701 caaaggaaag tatcgcaacc gggacggccg ctgccgttgc aggcggcttt gtcgcggttg
     2761 cctgcatgcc gaacacaaag ccggcgctgg actctcctga agtccttagg acccttctgg
     2821 aggatgtgga tcggaccggt gtcgcgcgcg tctatccaat cgctacgatc acacgaggcc
     2881 gtaccggtga tgaggcggtg gattttgacg ccgtgatcga cgcgggcgcc atcggctggt
     2941 cggacgatgg tgatacgacc gcgaacagtg cgatcatgcg tgaggtactt gtcgcttcga
     3001 ggcggctcaa ccggccggtc atcgttcatt gcgaggacaa ggcgattgcc acaggagcca
     3061 tgcacgaggg cgaggtatcg agaaagctgg gcattgaggg cattcctgca gcggccgagg
     3121 agatcatcat cgcgcgagac ctgatgctgg cggaactgac cggtggctgg ctgcacgtct
     3181 gccatgtcag cacgggacgc ggtattgagc tgatccacga agcgaagcag cgcggtgtca
     3241 acgtgactgc ggaggtcatg cctcatcatc tcgtcatgag cgacgagtgg gttgccggat
     3301 ctcgggagtt gcacaacacg gatgaagcag ccggagccca ggcgcagccc tgtgacccca
     3361 ataccaaagt caatccgccg ctccgctcac aggacaacac gcgtaaactc cttaaagcgc
     3421 tgcaggacgg atggttcgat tgtttcggca ccgatcacgc tccgcatgct gatcccgaga
     3481 aatcggtgga ataccagcaa gcagcgttcg gactgagcgg gctggagttt gcgttcccgc
     3541 tttcctacgc ccttgtacgc gccggtcatc tcggcgtggc tgatctcgtc cgccgctgga
     3601 cggtggagcc ggcaaagatc ctcggcgtcg atccaatgag tatccgggag gggtcggtgg
     3661 ccaatatcac ggtgttcgat cctgaggaat cctggacggt gacccctgaa gcactcaaga
     3721 caaaaagcaa gaacacgccc ttgcttagta tgacactgcg aggcagggcg gtattaacga
     3781 ttgtggatgg ggaggttcag caccgtgcct gatgcgaaaa cgcgatatgc gccagagtgg
     3841 gacgcggtcc ttgccttggc cgatggcaag gtgtttaggg gcaaggggtt tggtgccggc
     3901 atcgacggct cgggcgaagt tgtcttcacc acgtcaatga ctggttatca ggaggtctgc
     3961 accgatccct cgttccgggg ccagatcgtg tgcatgacct atccattgat cggtaactac
     4021 ggtgtgacgc aggaggataa tcagtcacgc cggccatgga tcgccgggct agtggttcat
     4081 gaggttgctc cccagccgtc gaactggcga tcggagggca cgcttgatga ctacctccgg
     4141 cgctacaacg tccccggcat atcgggtgtc gacacgcgcg cgctcacgcg tcacatccgc
     4201 tcggtagggg acatgcgggc agtgctcgtc cataacgcga aggacatgac cgaagaggaa
     4261 atcgtcgctc gtgcgcaggc ggccaagctg ccgggtgagc gtgacgttgt tggcgacgtg
     4321 atgaacgccg aagaggccac cttcggtgat gagaacgccg gcccgcatgt cgcggtggtg
     4381 gactgtggcg tcaagatgaa catcatcaac tccctgacga gccggggggc acgcgtcacg
     4441 gtgctgccct atgggacttc ctacgatcgg gtgcgggagc tcgatcccga tggcgtgatt
     4501 gtctcgcctg gaccgggaga tcccgccaac ctggacgagg ggttgggcat cgtgcggtca
     4561 ttgctcgagg accggcgacc ctatttcggc atctgccttg gacaccagct gctcgcgcgg
     4621 gcgattggtg ccgagacagg caagctcaag ttcgggcacc gcggtggaaa ccaacccatc
     4681 atggacgacc ggagcgggcg ggtgacgatg accgcacaga accacagctt tcaagtgaac
     4741 gcgggctccg tgcccaccgg tgacgggtgg caggtcgcgc tgcgtaatct taacgatggt
     4801 tctgtcgagg ggctggcaca tcgggacttg ccagtgcttt ccgtgcagtt ccatccggaa
     4861 gcggcgcccg gtccagccga caatggcgtc ctcttcgacg aatttctgga gatgatctcg
     4921 gttccgcagc aggatcacag gggaaagaac taacgtgaca gcaggatccc aggaacatgt
     4981 ctccaggcgc cccgagagcg tgctcgtgat cggctccggc ccgatcgtca tcgggcaagc
     5041 agcagagttc gattacgccg gtacccaggc gtgtcaatcg ctgcgggaag aagggatccg
     5101 gacgatcctc gtgaactcca acccggccac gatcatgaca gacgaggata tcgcggacgc
     5161 tgtctacatt gagccactga cgcctgatgt catccggcgg gtcatacagc gcgagcgtcc
     5221 cgaagggttg ctgcccacgc tcggcggaca gacggggttg aacctggcag ttcaggtttc
     5281 cgagctgggc atcctggatc ggtacaacgt ccggctgctt ggaacgccac tgtcagcaat
     5341 ccagaaggcg gaagaccgcg agctcttcaa ggatctgttg aacgagatcg gggagccggc
     5401 gatcgagagc gaggttatcc actccctgga agaggcacgg gagttcgtaa agcgggtgcc
     5461 gttgccactc gtcatccgtc ctgcgtacac cctcggcgga actggcggcg gcgttgccta
     5521 tacggaccag gacctggagc ggatcgtggc ctccggcctc cgcgccagcc cgatccgcca
     5581 ggtgctgctg gagcgttccc tgatgggttg gaaggagatc gagtacgagg tcatgcgcga
     5641 tagcgcagac acgtgcatta cgatctgcaa catggagaat ttcgatccta tgggggtgca
     5701 taccggcgac agcatcgtcg tcgcaccctc gcagaccctg tcggatcgtg aataccagat
     5761 gctgcggacg tccgcgctga acatcatccg cgcgctcggg atcgaaggcg gttgtaacgt
     5821 ccagtacgcg ttgaatccgc attcgatgga gtacggcgta atcgaggtga acccgcgtgt
     5881 gagccgctcc tccgcgctgg cctccaaggc gacagggtac cctatcgcgc ggatttccac
     5941 caagatcgcg atcggaaagc ggctggacga gctgaccaac gtcgttactg gtaagaccac
     6001 ggcggcgttt gagcctgcac tggactatgt tgtcgccaag attccccgct ggccgtttga
     6061 caagttcccc cgtgctgacc gcagcatcac cacccagatg aaggccacag gcgaagtgat
     6121 ggcaatcgat
//
LOCUS       DASYWM010000331         8418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_412720, whole genome shotgun sequence.
ACCESSION   DASYWM010000331 DASYWM010000000
VERSION     DASYWM010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412720"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VGR29_12995"
     CDS             <1..598
                     /locus_tag="VGR29_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074539.1"
                     /translation="LLQDVLQRHGNSEPLLYQEIGHWLGREHNMKARAWYGVPFSSFM
                     DAAQEGGYVTLTTSGGNSYASLPSTSIEDQIIDDGDEDEEEIDTGAAVRLESLQSEER
                     RALFDALRELDNDPRLKYFTFRTILRHLLSTSALPRLNETQIRSLLNDLANREPPILI
                     RTTRRGRNPSGGTYTFAGFTLTPDEDALRVDETEYDSD"
     gene            complement(748..2028)
                     /locus_tag="VGR29_13000"
     CDS             complement(748..2028)
                     /locus_tag="VGR29_13000"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV2074540.1"
                     /translation="MAVGTITRDEAQALAVIAQRLDRRSLRRRPTRSDVLDIFEHLGT
                     IQLDTISVISRSHETVLWSRLGSYDCNFVTDLYAEDSALTEYLGHAASILPTDLLPLF
                     RPYMEKARASHAGWSAQPESQAVMRRVIDRITAEGPITSRDFEPPPEATRAGQWEWWG
                     NKPERQALATLWIRGEVMVHKRDRGFARYFDLSERVVPGFWEGEVLSTDTQQRALLGH
                     ALRALGITTARWASDYFRTGGPPHVPIARVRELLKEFVEEGVAVPVEVPGIAEPVWMD
                     AALQERLELLRERKARPTLTTLLSPFDNLIWNRSRGEQLWDFFYRLECYVPEPKRQYG
                     YYSLPILHRGKIVGRLDPSFRRKTEVLTIKALHLEQWVRPSETLARSIAGAIEDLLAF
                     LGGTPGSWILAETSGQAITDLLRPYAGDVTVTRE"
     gene            2190..3296
                     /locus_tag="VGR29_13005"
     CDS             2190..3296
                     /locus_tag="VGR29_13005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2074541.1"
                     /translation="MRVALVHDYLTQYGGAERVLEVLHERYPDAPVYTSLYDPNHLPD
                     SFKSWDIRVSMLGSIPGSARTHRLWAPLYPAIFGHVGFHAIKDVDIVIADTSAWAHHT
                     RPRHDVPVIGYCHSPARFLYGDPNYLGAARIPAPLRPASSGLFTALRWLDRRAAERLA
                     HVVANSGAVRERILNDWGVDSTVIHPPVNIDRFRPAEPVDAEDWYLVVSRLVPHKWID
                     RAVRACTIANKRLKVIGSGRSEHALRRIAGPTIEFLGERSDAYVVDHMQRCRALILPG
                     IEDFGMTSIEAQAAGRPVIAARGGGALETVIENVTGLLFEPRNDDELVGLLHHEQTWD
                     SGVIQQHTRQFGTDVFLEKMNTVVQDVLCEAARP"
     gene            complement(3476..4234)
                     /locus_tag="VGR29_13010"
     CDS             complement(3476..4234)
                     /locus_tag="VGR29_13010"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEV2074542.1"
                     /translation="MPTSLPASSSPMAVTVVIPTYNEFENIDALVEEVLTLEPVPQVL
                     IVDDSSPDGTGELADQLAREHPGQVFVLHRTEKEGLGRAYIAGFQRALELGSSHIAQM
                     DADHSHRPSDLQIMIDVAAETGADLVLGSRYIAGGRTVGWPWHRKLISRAGGFYAGTI
                     LGVPIRDLTGGLKLWTSETLRDIDLMSIHSDGYSFQIETTYRALQRKHMVEQVPITFT
                     DRVAGASKLSRRVVIEAALMVWPLRLRQFFRRPW"
     gene            complement(4234..4617)
                     /locus_tag="VGR29_13015"
     CDS             complement(4234..4617)
                     /locus_tag="VGR29_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2074543.1"
                     /translation="MSSRILVVEDQDDVAQLIDVVLKGEGYTVAIARDGAQGLMMSRD
                     WQPDLILMDIMLPGVDGGTLIARLRQEPETADLPIIAMSASRTLRDRTPELQADALLS
                     KPFDVDALLVQVQFLLTRGRGDGDA"
     gene            complement(4726..6069)
                     /gene="murA"
                     /locus_tag="VGR29_13020"
     CDS             complement(4726..6069)
                     /gene="murA"
                     /locus_tag="VGR29_13020"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV2074544.1"
                     /translation="MSVFPETTIADTAVEERLLKIRGGNVLHGEVRISGAKNAALKAL
                     AASLLTTEEVVLNNVPMIADVLSMAELLRAFGAEVDIDKEHDRVAVRATEIVRTDAPS
                     HLFKATRASVVVTGPMLAREGEISFYMPGGDQIGKRPIDMHLRGFQRMGTEIIRNAEG
                     IQAKVAKLQGARIYMDYPSHTGTEAIMMASTLAAGTTVIVNASAEPEIVWLGNMLNRM
                     GARISGLGSPIITVEGVERLRGVSEIVIPDRLEAATYAIAAVITGGEVTLQRIFEPHL
                     LPITEKLLEAGAEVWHRDGRMLIRAGKELRSVDIQTLPFPGFPTDSQSTFVPLLTQAT
                     GVATVHERVYEDRLRYTDELVKMGADIRVARFGEKGEFLATSAEIHGPTRLRGTRVAS
                     LDIRSAVSLVLAGLVAEGETQLTDVYHIDRGYADFVNKLNELGADLTDTGEPAKV"
     gene            complement(6285..6716)
                     /locus_tag="VGR29_13025"
     CDS             complement(6285..6716)
                     /locus_tag="VGR29_13025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV2074545.1"
                     /translation="MAKLSMELVTGERVVYQADDVDMVVAPGSEGTLGILPNHAALIS
                     LLSAGEVRVKKGNQEDSLIVFGGFVEVLDNRVIILADSAERAEEIDLERAVRARDAAE
                     VSLRNESDRVSVAEADAALRRAALRLRIGERRRRNSSGAPR"
     gene            complement(6779..8218)
                     /gene="atpD"
                     /locus_tag="VGR29_13030"
     CDS             complement(6779..8218)
                     /gene="atpD"
                     /locus_tag="VGR29_13030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV2074546.1"
                     /translation="MAAPATAPQQQKTGATGTIVQITGVVVDAEFPPDQLPQIYNALE
                     TDLPDGRRLVLEVQQHLGDDRVRAVAMSTTDGLRRGQEVLDMGQAIAVPVGQATLGRI
                     FNVTGEPIDELGPINATEFRPIHQEPPSFEEQSTAVEVLETGTKVIDLIAPFTRGGKI
                     GVFGGAGVGKTVIITELIRNIAAEFGGYSVFCGVGERTREGTALWGELAESGVRDQTA
                     LVFGQMNEPPGARLRVSLTGLTMAEYFRDEGRDVLVFIDNIFRFVQAGSEVSALLGRM
                     PSAVGYQPTLANEMGQLQERITSTKTGSITSVQAVYVPADDYTDPAPSSVFAHLDATL
                     ALERSIAERGLFPAVDPLSSTSRILDPLIIGQEHYDVARAVQQILQRYRDLQDIIAIL
                     GVEELSDEDKQIVARARRIELFMSQPYFTAEQFTGNPGRFVSLADTVRSFKEIVDGKH
                     DHLPESVFRYKGSIDEVVEAAERLEAENR"
     gene            complement(8308..>8418)
                     /locus_tag="VGR29_13035"
     CDS             complement(8308..>8418)
                     /locus_tag="VGR29_13035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV2074547.1"
                     /translation="KELVDDLDLAFNKARQAQITNEVAEISAGANAFRTD"
BASE COUNT         1649 a         2648 c         2485 g         1636 t
ORIGIN      
        1 attattgcag gacgtccttc agcgccacgg caatagtgag ccgctgctgt atcaggagat
       61 cgggcattgg ctgggccgcg agcacaatat gaaagcgcga gcgtggtacg gcgtcccatt
      121 cagcagtttc atggatgccg cgcaagaggg cggatacgtt acgctgacga cctcgggcgg
      181 caacagttac gcctcgctcc cctcaacaag tatcgaagat caaatcattg atgatggcga
      241 cgaggatgaa gaggagatcg acactggcgc cgccgtccga ctggaatcgc tacagtccga
      301 ggagcgcagg gccctcttcg atgcactcag ggagctcgac aacgaccccc gcctgaaata
      361 cttcaccttc cgcacaatct tgcggcatct tctctcgacc agcgcgcttc cccgcctgaa
      421 cgagacgcag atccgcagcc tcttgaacga tcttgcgaac cgcgagccac cgatcctgat
      481 ccgcacaacc agacgcggac gcaatccctc gggcggtacc tacacctttg ccgggttcac
      541 gctcacgcca gatgaggacg cgctccgggt ggatgaaacg gaatacgatt ccgactagag
      601 cggcgtgccg gtcgacacaa tgagcgatgt gatgcgcagc tgagcgacga atgtcgtcag
      661 gaatggcagg gtaagcggag tgccccgccc ctcagatgtg tctcacggcc acaggaggtt
      721 cctggctctc cccaaccccc tcgcctacta ctcccgcgta actgtgacgt cccccgcata
      781 cgggcgcaac aaatcagtta tggcctgtcc actggtttcc gccaagatcc atgaacctgg
      841 tgtgcctcca aggaatgcaa gcagatcctc gatggccccg gcgatcgagc gtgcgagcgt
      901 ctcacttggc cgtacccatt gctcaaggtg cagcgcctta atcgtgagga cctctgtctt
      961 gcggcgaaag ctggggtcaa ggcgtccaac aatcttcccc cggtgcagaa tcggcaggga
     1021 atagtagccg tactgccgtt tgggctcggg cacgtagcac tccaaccgat agaaaaagtc
     1081 ccatagctgc tccccccggg accggttcca tatcagatta tcgaaggggg aaagaagtgt
     1141 cgtgagcgtc gggcgtgcct tgcgctcacg gagcagttcc agccgctcct gcaatgccgc
     1201 atccatccac accggctcag cgatcccggg aacctcgacg ggaacggcga ccccctcttc
     1261 gacaaactcc ttcaatagct cgcgcaccct ggcaatagga acatggggcg gaccaccggt
     1321 ccggaagtaa tctgaagccc aacgtgcggt ggtgatcccc aatgcccgca atgcgtggcc
     1381 taacagcgcc cgctgctgtg tgtcggtgga gagaacctcc ccttcccaaa agcctggaac
     1441 tacccgctct gaaagatcga aatatcgcgc gaagccccgg tctcgtttgt ggaccatgac
     1501 ctcgccgcga atccacaacg tcgcaagggc ctggcgctcc ggtttgtttc cccaccattc
     1561 ccattgcccg gcccgcgtag cctccggtgg cggctcaaag tctcgtgagg tgatcggccc
     1621 ttccgcagta atccgatcta tgaccctgcg catgaccgcc tggctctcag gttgcgcgct
     1681 ccatcccgcg tgagacgcac gcgccttctc catatagggg cgaaagagcg gtaacagatc
     1741 ggtcggcagg atcgatgcag cgtgccccag gtattccgtc agcgcactgt cctcggcata
     1801 gagatcggtt acgaaattac agtcataaga cccgagccga ctccagagga cggtttcgtg
     1861 agagcgggag atgacagaga tggtatcgag ctggatggtg cccaggtgct cgaagatatc
     1921 caggacatcg cttctggtgg gtcggcggcg aagactccga cggtccagcc tctgcgcaat
     1981 gaccgccagt gcttgtgcct catccctggt gattgtccca accgccactc atgactcctc
     2041 tctgcatcgt cgagcgagta catccgcatg tcgagcgtcg acgtttccct gcgaatacac
     2101 gctgcacatg ctatcgcaca ccacggacac ctcctgtccg cgttatgctt gctgcggaat
     2161 attgtcgttc tggaggattc aacctatcca tgcgcgttgc gctcgttcac gactacctaa
     2221 cccaatacgg cggcgccgaa cgtgtcctcg aagttctcca cgaacgctat cctgacgcac
     2281 ccgtctatac ctcgctctac gatccgaacc acctgcctga ctccttcaag agttgggata
     2341 tccgggtgag catgctcggc agtatccctg gatccgcgcg aacgcatcgg ctttgggcac
     2401 cgctttatcc tgctatcttc gggcacgtcg gattccatgc gatcaaggat gtcgatattg
     2461 tgatcgcgga caccagcgca tgggcccacc atacgcggcc aaggcacgac gttccggtca
     2521 ttggctattg tcactcgcct gcccgcttcc tctacggcga ccctaactac cttggcgctg
     2581 caaggattcc tgcgccgctt cgaccagcga gcagcggcct ctttacggcc ctgcgctggc
     2641 tggatcggcg tgcggcagag cgtcttgcgc acgtcgtcgc taactcaggc gccgtccgtg
     2701 agcgcattct gaacgactgg ggcgtggact ccacagtgat ccacccacca gtcaatatcg
     2761 atcgattccg acctgcagaa ccagtggatg ctgaggattg gtatctcgtc gtttcccgac
     2821 tcgtcccgca caagtggatc gatcgggcgg ttcgtgcatg cacgattgcc aacaagcggc
     2881 taaaggtgat cggaagcgga aggtcagaac atgcgctccg gcgcattgcc ggtcccacga
     2941 tcgagttcct gggtgagaga tccgatgcct acgtcgtgga tcacatgcaa cgctgccgcg
     3001 cgctcattct tcccggtatt gaagacttcg gcatgacatc gatagaggcg caagccgccg
     3061 gccggcctgt tatcgccgcg cgtggtggcg gagcgttgga aacggtgatc gagaacgtga
     3121 ctggcctgct cttcgaacct cgaaacgatg atgagctcgt tgggctgctc catcacgagc
     3181 agacctggga tagcggggtt atccagcagc acacgcgtca gtttgggact gatgtcttcc
     3241 tggagaagat gaatacagtc gtgcaggacg ttctctgtga ggccgccagg ccatagcttc
     3301 tctcgcatag gagaggcgac taccgaacgg gcttgtcgcg cggaagaagt actgcgacca
     3361 tatccagggt ttgagccacc cgtaggtagc cttgggtctt ccgaccgtca cgcccgccgt
     3421 ttgacacgtc catcgaagga cgcagggtgt tatccggcaa gaaatggaac ggaccctacc
     3481 acggacggcg aaagaactgt cgcagcctga gtggccagac catcaaggca gcttcaatca
     3541 ccacgcgccg cgaaagcttg gacgctcccg ccacccgatc cgtgaacgta atcgggactt
     3601 gctcgaccat atgtttccgc tggagcgcgc ggtaggtcgt ctcgatctgg aagctgtatc
     3661 cgtcggaatg aatgctcatc aggtcgatgt cacggagcgt ctcgctcgtc caaagcttca
     3721 gacctcccgt gagatcgcgg atcggcaccc caagtatggt tcccgcgtag aaccctccag
     3781 cgcggctgat caacttccga tgccagggcc atccgactgt ccgtccaccg gcgatatacc
     3841 ggcttccaag aaccaggtcg gcgcccgtct cggcggcgac atcgatcatg atctggagat
     3901 cggagggacg atgcgaatga tcggcgtcca tctgcgcgat gtgagacgac cccagttcca
     3961 gtgcccgctg gaaccccgcg atgtaggcgc gtcccagccc ttccttctcc gtccggtgca
     4021 gaacaaacac ctgtcccggg tgctcgcgcg cgagctgatc cgccagctcg ccggtaccat
     4081 cgggcgagga atcgtccacg atgagcacct gcggcactgg ttccagggtc agcacctctt
     4141 cgacaagcgc atcgatgttc tcaaactcgt tgtacgtggg aatgacgacc gtcacggcca
     4201 ttggggagct tgaggccggg agagaggtag gcatcaggcg tcgccgtcgc cccggccgcg
     4261 agtgagcaga aactgcacct gaaccagcag cgcgtcgaca tcgaacggct tggagagcaa
     4321 ggcgtccgcc tgcagctcgg gcgttcggtc cctcagcgtc cggctggctg acatggcaat
     4381 gattgggaga tcagctgtct ccggttcctg gcggagacga gcgatgagcg tgcctccatc
     4441 aacacccgga agcatgatat ccatcaggat gaggtccggt tgccagtcac gggacatcat
     4501 gaggccctgc gcaccatccc gagcaatggc aacggtgtat ccctcgccct tcaagacaac
     4561 atcgatgagc tgggcgacat catcctgatc ttcaacgacg agaattcgtg acgacacgcg
     4621 attgcttcct tacgtaacgg gcgcctcaag gcgccggcga ttctccgttc aacctactct
     4681 accgcaatgt acgaacgcaa cttccggacc ggcctcgtcc agcgtctata cctttgcggg
     4741 ttcgccggta tccgtgagat ctgccccaag ctcgttgagc ttgttgacga agtcggcata
     4801 gccgcggtcg atatgataaa cgtcggtaag ctgggtttcc ccctcagcga ccagcccggc
     4861 cagcacgaga ctcacagccg aacgtatatc cagcgacgcg acgcgggtgc cccgaagcct
     4921 ggttggtccg tgaatctccg cagatgtcgc aaggaactcc cccttctcgc cgaagcgggc
     4981 gacccggatg tccgcgccca tcttcaccag ctcgtccgta tagcgcaggc gatcctcgta
     5041 cacccgctcg tgcactgtag cgacgcctgt cgcctgggtc agcagcggaa caaaggtcga
     5101 ttggctatcc gtgggaaaac cggggaatgg cagcgtctgg atatcgacgg accgcagttc
     5161 ctttccggcc cggatcaaca ttctcccatc gcgatgccag acctccgcgc ccgcttcgag
     5221 cagcttctcc gtgataggta acaaatgggg ctcaaaaatt cgctggagcg ttacctcacc
     5281 gccggtgatg accgccgcga tcgcgtaagt cgccgcctca agccggtctg gaatgacgat
     5341 ttccgagacg ccacgcagcc gctcaacacc ttcgacagtg atgatgggag agcccagccc
     5401 gctgatgcgc gcgcccatcc ggttcagcat attgccaagc cagactattt ccggttccgc
     5461 cgatgcattg acgatcaccg tcgtgccagc cgccagggta gaggccatca tgatggcttc
     5521 ggttcccgta tggctgggat aatccatgta aatgcgggcg ccttggagct tggcgacctt
     5581 ggcctggatt ccctcggcat tgcggattat ctccgtaccc atccgctgga agccacgcaa
     5641 gtgcatatcg atagggcgct tgccgatctg gtcgccaccg ggcatgtaga aggagatttc
     5701 gccctcccgc gccagcatcg gcccggtgac caccacagat gcgcgggttg ccttgaagag
     5761 atgtgacggg gcgtctgtgc gaacgatctc ggtcgctcgt accgcgacgc ggtcgtgctc
     5821 cttgtcgatg tcaacctccg cgccgaacgc ccgcagcagc tccgccatgg agagcacatc
     5881 agcgatcatg gggacgttat tcagtacgac ctcttcagtt gtaagcagcg acgcggccag
     5941 tgccttcaac gcggcgttct ttgcgccgct gatacgaacc tcgccatgaa ggacgttgcc
     6001 tccccggatt ttgagcaggc gctcctctac cgcggtgtca gcaatggtgg tttcggggaa
     6061 gacggacatg gatgcgaccc ttccggtcat gattgaaagg tggtggttga atcggtggcg
     6121 gcgcgtgaag accaccgcac gttgtctcgc aacaacgggc ccatggcgtt ccggcaacgg
     6181 cccggacaag tatagatgtg ctaccccatg ggacaagcac gcacagagcc ggcgatgtcg
     6241 gtgtaacatc gccggctccg ccctcttccc cttgataggt acgtttagcg tggcgctcca
     6301 ctgctgttac gacgacggcg ctcgccgatc ctgagccgga gcgctgcgcg gcgaagcgcc
     6361 gcatcggcct cggcgacgct cacccggtcg gactcgtttc gcagtgacac ctctgccgca
     6421 tcgcgtgcgc gtaccgcccg ctcgaggtca atctcctccg cccgttccgc actatctgcg
     6481 aggatgatca cgcggttgtc gaggacctca acaaatccgc cgaagacaat cagggagtct
     6541 tcctggttgc ctttcttgac acgcacctca ccggcggaaa ggagggagat gagcgcggcg
     6601 tggttcggca agatgccgag ggttccctcg gagccaggtg caaccaccat atctacgtca
     6661 tctgcctggt agacgacacg ctcacctgtt acgagctcca ttgagagctt tgccacggac
     6721 aacctcctgt agcgaggagg tcacaccgtt gttcggcgcg acctccattc tcgccccctt
     6781 accggttttc ggcctcaagg cgctcggcgg cttcaacgac ttcatcgatg ctgcccttgt
     6841 aacggaagac gctctccggc aggtgatcgt gcttgccgtc gacgatctcc ttgaaggagc
     6901 gaaccgtgtc tgcaagcgag acgaagcggc ccgggttgcc ggtaaactgc tcggctgtga
     6961 agtacggctg ggacatgaag agctcgatgc ggcgggcacg ggccacgatc tgcttgtcct
     7021 cgtcgctcag ctcctcgaca ccaagaatgg cgatgatgtc ctgcaggtca cgataacgct
     7081 gcaagatctg ctgcacggcg cgggccacat cgtagtgctc ctgcccgatg atcagcggat
     7141 cgaggatacg gctagtcgac gaaagtggat cgaccgccgg gaacagaccg cgctcggcaa
     7201 tcgagcgctc caacgcgagc gtcgcgtcaa ggtgagcgaa cacggacgac ggggccggat
     7261 cggtgtagtc gtcagctggc acgtagacgg cctgcacgga cgtaatggag ccagttttcg
     7321 tcgaggtaat gcgttcctga agctgaccca tctcgttcgc gagggtcggc tgatatccca
     7381 cagcgctcgg catacgcccg aggagggccg atacctcgga gcccgcctgc acgaaccgga
     7441 agatgttgtc gataaagacg agcacgtcgc ggccttcgtc gcggaagtac tcagccatgg
     7501 tcaggccggt aagcgacaca cggagacggg ctcccggcgg ctcattcatc tggccaaaga
     7561 cgagggcggt ctggtcacgc acgccggact cggcaagctc gccccagagc gcggttccct
     7621 cacgggtccg ctcacccacg ccgcagaaga cggagtagcc accgaactcg gccgcaatgt
     7681 tacggatcag ctcggtgata atgactgtct tgccgacgcc ggcaccgccg aagacgccga
     7741 tcttgccgcc ccgggtgaag ggggcgatca ggtcgatgac cttggtgcct gtctcaagca
     7801 cctcaacagc cgttgactgc tcctcgaagg agggcggctc ctggtgaatc ggccggaact
     7861 cggtcgcgtt gatgggtccc agctcgtcga tcggttcacc cgtcacattg aagatacggc
     7921 cgagcgttgc ctgcccgacc ggcacggcaa tcgcctgacc catgtccagg acttcctggc
     7981 cacgacgtag accgtcggtg gtgctcatgg caacggcgcg aacgcggtcg tcaccgagat
     8041 gctgctgcac ctcaagcacg agtcgccgcc catcggggag gtctgtttca agcgcgttgt
     8101 aaatctgtgg gagttggtct ggcgggaatt cagcgtcgac cacgacaccg gtgatctgga
     8161 cgatcgtgcc ggtagcgccg gttttctgct gttggggtgc ggttgctggt gcagccatgt
     8221 tgcgttcctt acctgaagcg ggtgccgtga ggctgtaacc gctcatgcct caaatctctc
     8281 tttgcaatat cgtcgtgcgg tacggcccta gtcggtccgg aaggcgttcg cgcccgccga
     8341 gatctcggcg acttcgttgg tgatctgtgc ctgacgcgcc ttgttgaaag caaggtccag
     8401 gtcgtcaacg agctcttt
//
LOCUS       DASYWM010000332         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_544159, whole genome shotgun sequence.
ACCESSION   DASYWM010000332 DASYWM010000000
VERSION     DASYWM010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544159"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VGR29_13040"
     CDS             <1..820
                     /locus_tag="VGR29_13040"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEV2074548.1"
                     /translation="PVEPTTGRHGGYALRPGFRLPPMMFNADEALGLAIALLMSRTTR
                     GAELPPGVESALAKIERVLPPELSRRIHALREGVSLPVDDHLGSPSFPDPHVLAKLAQ
                     ACITRQRCWMRYSRPDGDESAREVDPYGIGAFFGRWYLHGWCHLRNDHRTFRIDRIRR
                     VDVLPQTFEAPEEMDVIAAIESSLALSWTTWRIEVEVEASVSDVREYVPRSFAIVEEI
                     GEGRSRIRGSSNTLEWFVLRIAELPFRMRIIEPPELRDAFRRCAERMLEIAERP"
     gene            complement(855..1292)
                     /locus_tag="VGR29_13045"
     CDS             complement(855..1292)
                     /locus_tag="VGR29_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2074549.1"
                     /translation="MRAAQEADIPTLYELHWSAMHDYVDRTWGWDEDDQFARFHHYAS
                     ASPLQVIEVDGQPAGFIHIEHTADAVEIVNIELASTAQGRGIGTELLKQAIADAGKKG
                     VPLRLQVLKANPAAQRLYVRIGFEKTGETETHVRMQWGGGPGK"
     gene            1544..>3036
                     /locus_tag="VGR29_13050"
     CDS             1544..>3036
                     /locus_tag="VGR29_13050"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEV2074550.1"
                     /translation="MTMDLGAWLLLLSTLLAGAVLLGVLARRANVPLTVVLALVGFLA
                     GWLGNLQSPIGGERFEEVLVFVFLPVLVFEAALGLSTRAFFRNLVPILVLAIPALAVS
                     AICVGLSLYIGLDTPLIPALLFGALISATDPVAVVAIFRELGVPRRLLTLVEGESLLN
                     DGVAIVLFTILLAAALGGDLNAFEGVWEFIVVCFGGVAIGAGVGFLAALALPWLDRLS
                     AAALSLAVAYGGFVLADDVLGFSGVTATVAAGLVLGGSAPIRATAPVRELWEGLWEAL
                     GYVANALLFLLIGLAIDPSLLTENIGAIGLAIVVVLLARAIAVVPLMVLIERVGNIPP
                     VGMRNQAVLIWGGLRGGVALALALALPEELAQRDQFVAMTGGVVLATLLLNATTIGML
                     VRRLGLDEPSPADRFLAGGARLSAITAARERLADLQLEDPIVMSELDQAEEETRAELA
                     RLSLDEAAELQLVTRRGLAVERETYQHLSDVGLLPPTATRVLLHEVD"
BASE COUNT          470 a          949 c          944 g          673 t
ORIGIN      
        1 gccggttgag ccgacgacgg gccgacatgg cggctacgcc ctgcggccag ggttccgcct
       61 gccgccaatg atgttcaatg ccgacgaggc acttgggttg gcgattgccc tgctgatgag
      121 ccgtacgacc cgaggtgcgg agcttcctcc aggcgtcgag agcgccttgg ccaagatcga
      181 gcgcgtactc ccgccagagc tatcgcgccg gatccacgcg ttgcgggaag gcgtatcgct
      241 tccggtggac gaccatctcg ggtcgcccag cttccccgac ccacatgtcc tcgccaaact
      301 ggcccaggcg tgcatcaccc gacagcgctg ctggatgcgc tacagccgac cagatggtga
      361 cgagagcgct cgggaagttg acccctatgg aataggcgcc ttctttgggc gctggtatct
      421 ccatggctgg tgccatcttc ggaacgatca ccgcaccttc cggatcgacc gtattcgccg
      481 ggtggatgtc ctgccgcaga ccttcgaggc gcccgaggag atggacgtga tcgcggccat
      541 cgaatcgtca ctggccctgt catggacgac gtggcgaatc gaagtcgaag tggaggcatc
      601 cgtgtcagac gttcgcgaat atgtgccacg gtccttcgcg atcgtcgagg agatcggtga
      661 agggcgcagc cggattcgag gatcatcgaa cactctggag tggtttgtgt tgcgaatcgc
      721 cgagctgcca ttccggatga ggatcatcga gccaccggag ctccgcgacg ccttccggcg
      781 atgcgccgaa cgcatgctgg agatcgcgga gcgcccgtag cagcgagcaa tggaaatggg
      841 gtggctagtg tgccctactt cccaggccca ccaccccatt gcatgcgtac atgagtctcc
      901 gtttcgccgg tcttctcaaa gcctatccgt acatagaggc gctgcgcagc ggggttcgcc
      961 ttgagcacct gcagtcgcag aggcacccct ttctttcctg catctgcaat ggcctgtttc
     1021 agcaactccg tgccaatacc tctgccttgg gcggttgatg ccagctcgat gttgacaatc
     1081 tccaccgcat ccgccgtgtg ctcgatatgg atgaacccag ctggttggcc atccacctcg
     1141 atcacctgca acggcgatgc ggacgcatag tgatgaaatc gtgcgaactg atcatcctca
     1201 tcccagcccc acgtccggtc gacatagtca tgcatcgcgg accagtgcaa ctcatacagg
     1261 gtggggatgt cagcctcctg tgccgccctc atgtgataca caacggccct ccatgccagc
     1321 catcccgatg atccggccat gccagaatgg cccatcgctt aacgaataag agttctcggg
     1381 cttgtacaca ggtcggttcc cggttcccac gatcaggaat actggatctt ctccggcaag
     1441 tgcctgttca cggcgctggt gcgggcgtat gctcggcgtg ataatacacg caattcatgc
     1501 atgtctttaa gggctatccg cttgcgcttg tcgtgggaga cacatgacca tggacttggg
     1561 tgcctggctc cttctcctct caacgcttct tgccggcgct gtactgctcg gcgtcctcgc
     1621 gcgtcgagcc aacgttccct tgacggttgt tctggctctt gtgggcttcc tcgccggctg
     1681 gctgggcaac ctccagagcc cgatcggggg tgaacggttc gaagaggttc tggtcttcgt
     1741 cttcctcccc gtgctcgtct tcgaggccgc tcttggcctc tccacccgcg ccttctttcg
     1801 taaccttgtt ccaatcctcg ttctggcgat tcccgcgctt gctgtctcgg caatctgtgt
     1861 cggtctctcc ctctacattg gcctcgatac gcccctgatc cccgcgctcc tcttcggagc
     1921 gctcatttcc gccaccgatc cggtcgctgt agtggccatc tttcgtgaac tcggtgtgcc
     1981 gaggcgcctg ctgacgcttg tcgaggggga gagcctcctc aacgatggtg tcgccatcgt
     2041 cctctttacg atcttgcttg cagcggcgct tggtggggac ctcaacgcgt tcgaaggagt
     2101 gtgggagttc atcgtcgtct gcttcggcgg cgtggctatt ggggcaggag tgggctttct
     2161 cgcggccctg gcgctgccat ggctcgatcg gctctcggcg gccgcactct ccctggccgt
     2221 cgcttatggt ggtttcgtgc ttgccgatga tgtgcttgga ttctcagggg tgaccgccac
     2281 ggttgccgcc gggctggtgc tgggcggctc tgccccaatc cgcgccactg cgccggtccg
     2341 ggaactgtgg gaggggctgt gggaagcgct tggctacgtc gccaatgccc tcctgttctt
     2401 gctgatcggt ctcgcaattg atccatccct gctgactgag aacattggtg ccatcggcct
     2461 cgcgatcgtt gtcgtgttgc ttgctcgcgc catcgccgtt gtgcccctaa tggtcctgat
     2521 tgagcgggtg gggaatattc cgccggtggg tatgcgcaat caggcggtgc tcatctgggg
     2581 tgggttgcgg ggcggcgtgg cgcttgcctt ggcgcttgcc ctgccggaag agctcgccca
     2641 gcgggatcag ttcgtggcga tgaccggtgg tgtggtgctg gccacgctgc tgctcaatgc
     2701 cacaacgatc ggcatgctcg tccgtcgcct tgggcttgac gaacccagcc cggctgatcg
     2761 ctttctggcg ggtggtgcgc gcctctcggc cattaccgca gcgagggaac gacttgctga
     2821 cctgcagttg gaagacccga tcgtcatgtc tgagctcgac caggccgaag aagagacgcg
     2881 ggcggagttg gcgcgcctct cactcgacga ggcagccgaa ctccagctgg tgacgcgccg
     2941 ggggctcgcg gtggaacggg agacctatca gcacctgagc gatgtgggcc tgctaccacc
     3001 aacggctacg cgcgtgttgc tgcatgaggt cgatga
//
LOCUS       DASYWM010000333         4162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_562773, whole genome shotgun sequence.
ACCESSION   DASYWM010000333 DASYWM010000000
VERSION     DASYWM010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562773"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="VGR29_13055"
     CDS             complement(<1..1165)
                     /locus_tag="VGR29_13055"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV2074551.1"
                     /translation="MISSAQTTERYPPIGDYAIIGDCRSAALVSCEGSLDWLCWPQFD
                     SPSIFASLLDAEQGGRFSLQPTDPFRTERHYLPGTNVLETVFHTDSGSVALRDVMTVA
                     SEEYKQDALVPEHEILREIEGLAGTVEVEIHYEPRPDYARRTVSLKQRGRLGVWCRAG
                     RAALVLHSDAPLDISSDKRSASSTIRVLAGERRFLSLTWSDDGPAVVPALGASARARL
                     EQSVEWWQEWSNRSTYKGPYREAVERSLLALKLMCFAPSGAVIAAPTTSLPEKIGGVR
                     NWDYRYCWLRDASYTVRALLELGYEEEAEAYCGWLLHATRLTWPELQVVYDVYGKARL
                     PEDELDHLEGYAGSRPVRIGNGAHDQIQLDVYGEVLDAIFRLVNGPDFFDRDTV"
     gene            complement(1200..2213)
                     /locus_tag="VGR29_13060"
     CDS             complement(1200..2213)
                     /locus_tag="VGR29_13060"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2074552.1"
                     /translation="MSNNMDEIPGGGQVVVVTGASSGVGRAVARAFGERGARVGLLSR
                     NEDALNAAAAEIRSAGGEALVCPTDVADSQAVERAAAQIEAQWGRIDVWVNCAMATVF
                     APVAEMTPDEYRRVTDVTYLGYVHGTLAALRRMRPRDQGTIVQIGSALAYRSIPLQSA
                     YCAAKAAIRGFTDSLRTELIHEGSSVRITAVHLPAINTPQAIRQRNKMPRQSQPVPPM
                     FDPEIIARQVVWASDHAPREMLVGTPTMKAVWAQKFIPGLLGRYLGKNGWDAQFTDEP
                     NRQDGDILFETLPGDPGAHGPYRDREQGPDLQMRLRPHARSLAAAVGVGCVLAIKAIG
                     RRA"
     gene            complement(2263..3606)
                     /locus_tag="VGR29_13065"
     CDS             complement(2263..3606)
                     /locus_tag="VGR29_13065"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEV2074553.1"
                     /translation="MQADPDEMIRRHRTQTLWMYFANIILGLWLLTSPAIFDYRSEQL
                     AWSDALSGALIVALGIVAFFPRGDFWGRWGICLVGTWLLFAPLIFWAPTAFVYVNDTL
                     IGGLVISFSVLAPMMPGKAHHMAMMQPGPDVPPGWTYNPSSWWQRGPIIALGLISFLI
                     SRYLAAYQLGHIDTVWDPFFKTGTARVLTSDVSKAWPVSDAGLGATSYLLEALSGFMG
                     GIRRWRTMPWMVVMFGVLVVPLGITSIILVILQPVMVGTWCTLCLTTALFMLVMIPLA
                     VDEIWAMVQFMLHTRREGKPMWRTFWIGGTIEGGSEDTRSPEFDGPRFPMVPSMVWGV
                     TIPGNLLISALIGFWLMAAPPVFDSTGTVANSDFLAGPLVAVIAVIAMAEVARPLRFI
                     NVVIGLWLVSTPWFLSGTTTGSTVNDIVAGLALIGLSLPRGSVRERYDGWQPYIV"
     gene            complement(3741..3938)
                     /locus_tag="VGR29_13070"
     CDS             complement(3741..3938)
                     /locus_tag="VGR29_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074554.1"
                     /translation="MVLHPPVMHMVLFTMAGFVITLIAEWLATDMLDWWAYAERMPTL
                     PLLQWMILPPLVIWFVQPQFA"
BASE COUNT          828 a         1308 c         1290 g          736 t
ORIGIN      
        1 gaacggtatc ccggtcgaaa aagtcagggc cattcactaa tcggaagatg gcgtcgagca
       61 cctctccgta cacgtccagc tggatctggt catgagcgcc attaccgata cgcacgggcc
      121 gagaaccggc gtatccctcg agatgatcca gttcgtcctc cggcaggcgc gccttaccat
      181 agacgtcgta gaccacctgg agctcgggcc acgtcagacg tgtcgcgtgg agaagccagc
      241 cgcagtaggc ttcggcttct tcctcgtagc caagctcgag gagtgcgcgg acggtgtacg
      301 aggcgtcacg cagccagcaa taacgatagt cccagtttcg tacgcccccg atcttctcag
      361 gaagtgacgt tgtcggcgcg gcgatgacgg cacccgacgg ggcgaagcac atgagcttga
      421 gcgccaggag gctccgctcc acagcctcac ggtacggtcc cttgtaagtg gagcggttgg
      481 accactcttg ccaccactcg actgattgct ccaaacgtgc acgggccgaa gcgccaagcg
      541 ccgggacgac cgccgggccg tcatcgctcc aggtgaggga gagaaagcgt cgctctccgg
      601 cgaggacgcg aatggtagaa ctggcggacc gcttgtcgga ggagatgtcg agcggcgcgt
      661 cgctgtggag gaccagcgct gcgcgaccag cacggcacca cactccaagc ctgccgcgct
      721 gtttgaggga aacggttcgc cgggcatagt ccggccgggg ctcatagtgg atctccacct
      781 cgaccgttcc ggccaggcct tcgatctcgc ggaggatctc gtgctccgga accagcgcgt
      841 cctgcttgta ttcctcggac gccacggtca tgacatcgcg cagggccacc gagccgctgt
      901 cggtatggaa gacggtttca agcacgttgg tgccggggag atagtgcctc tcagttcgaa
      961 atggatcagt cggttgtagt gagaagcgtc ctccctgctc ggcgtcgagc agggaggcaa
     1021 agatcgacgg actatcaaac tgtggccagc agagccagtc gagggagccc tcacaagaga
     1081 cgagtgctgc acttcggcaa tccccgataa tggcgtagtc gcctatggga ggatagcgtt
     1141 cggtggtttg ggcagaggag atcatgttcc ctcgcccccc gcactccacg gtgtcgtggt
     1201 catgcgcgtc gtccgatcgc tttgatcgcc agcacacatc ctaccccgac tgctgccgcg
     1261 agggaacggg catgaggacg cagacgcatt tgaagatccg gcccttgctc ccgatcacgg
     1321 taggggccat gcgcacccgg atcacccggc aatgtctcga acaggatatc gccatcctgt
     1381 cggttaggtt catccgtgaa ctgggcgtcc cacccgtttt tccccaggta ccggccgagc
     1441 aggcccggga taaacttctg ggcccaaacc gccttcatcg ttggagtccc gacaagcatc
     1501 tcccgcggcg catggtcgct tgcccagaca acctgcctgg cgatgatctc cgggtcgaac
     1561 atcggcggta cgggctggga ctggcgcggc atcttgtttc gctgccggat cgcctgcggg
     1621 gtgttgatcg ccggcagatg gacagctgtg attcgcacgc tgctcccctc atgtatcagt
     1681 tccgtgcgca gcgagtcggt aaaaccgcga atggcagcct tggcggcaca ataggcgctc
     1741 tggagcggga tggagcggta ggcaagtgct gatccgatct ggacgatggt cccctgatct
     1801 cgcggtcgca ttcgtcgcag ggccgccagg gtgccgtgga cgtaccccag gtacgtgaca
     1861 tcggtcacgc gtcggtactc atccggggtc atctcggcga ccggggcgaa cacggtcgcc
     1921 atcgcacagt tgacccagac atcgatccgg ccccactggg cttcgatctg ggcggcggca
     1981 cgttcaaccg cttgggagtc ggcgacatcg gtcgggcaaa ccagcgcctc tccgccggct
     2041 gaccgtatct ctgcggctgc ggcattgagg gcatcctcgt ttcgagacaa cagtcccacg
     2101 cgggcgccgc gctctccaaa ggcccgtgcg actgcgcgcc cgacaccgct ggatgcaccg
     2161 gtcacaacga ccacctgacc gccacccggt atttcatcca tgttgttgct caagtccatg
     2221 tccttcctga accgcgatac ggctcgccgt atcgtcgacc cattacacaa tgtacggttg
     2281 ccacccgtcg tatcgctcac ggacgctgcc acgcggcagg cttagaccaa tcagagcgag
     2341 gcccgcaacg atatcgttga cggtcgatcc cgtcgtggtg ccactgagga accacggtgt
     2401 ggagaccagc cagagcccga tgacgacgtt gatgaagcgg agtggacgcg cgacctcggc
     2461 catcgcgatg acggctatga cggccacaag ggggccggcg aggaagtcac tgttcgcgac
     2521 ggtgcctgtg gaatcaaaca caggcggtgc tgccatcaac cagaagccta tcaacgcgct
     2581 tatcagcagg ttcccgggaa tggtcacgcc ccacaccatc gatggaacca tcgggaaccg
     2641 gggtccgtcg aattcaggag atcgcgtatc ttccgagccg ccctcgatcg tgccgccaat
     2701 ccagaaagtt cgccacatcg gcttgccttc gcggcgtgtg tggagcatga actgcaccat
     2761 cgcccagatt tcgtcgaccg ccagtgggat catcaccagc ataaagagcg ctgtggtaag
     2821 gcagagggtg caccacgtgc cgaccatcac cggttgcagg atcaccagga taatgctggt
     2881 gatgccgagt gggaccacga ggacgccgaa catcaccacc atccatggca tcgttcgcca
     2941 gcgacgaata ccacccatga atcccgacag tgcttccagt agatacgagg ttgcaccgag
     3001 gcccgcatcg gagacgggcc acgctttgga gacatccgac gtcagaaccc tcgccgtgcc
     3061 ggtcttgaag aacgggtccc agactgtatc gatgtggcca agctggtagg ccgccaggta
     3121 gcgggagatc agaaagctta tgaggccgag tgcgataatc ggtccgcgct gccaccagct
     3181 ggatggattg taggtccaac cgggcggaac gtctggccca ggctgcatca tcgccatgtg
     3241 atgcgccttg ccgggcatca tcggggcgag cacggagaac gagatcacga gtcccccgat
     3301 caaggtgtcg ttgacgtaca cgaacgccgt gggcgcccag aagatcaacg gtgcgaacag
     3361 gagccaggtg ccgaccaggc agattcccca gcgaccccag aaatccccgc gcgggaagaa
     3421 cgcaacgata ccgagtgcaa cgatcaaggc accactcagg gcgtcactcc acgccagctg
     3481 ctcgcttcga tagtcgaata tggccgggct ggtgagcagc cacagaccca ggatgatgtt
     3541 ggcgaagtac atccacagtg tttgtgtccg atgtcggcgg atcatctcgt cgggatcagc
     3601 ctgcatcgcg gatccgggtc cggaatgatc cccgcccgtg ctcggagtga cgctaggcgc
     3661 gtccgcctta ccttccgggg agaatctgtt cccactcccg tgcggttcct gttgcatggc
     3721 tcaatgtccc attcatcgaa tcacgcgaac tgaggctgga cgaaccatat caccaacggg
     3781 ggcaggatca tccactggag caggggcagc gtcggcatcc gctcagcata cgcccaccag
     3841 tccagcatgt ccgtggctag ccactccgcg atcagggtta tcacgaaccc tgccatcgtg
     3901 aaaagcacca tgtgcatgac gggtggatgc agcaccacgc atgtctcgac gcaacgagcg
     3961 tgactggcgt gaatggggcc agagcgacta tcgcatcgcc gtgcgccccg cccgcattcc
     4021 cggtgagcac cagcagggcg catgatggct actctcccaa tgtgcgttcg acggtgaccg
     4081 cggggaacat cacgtggaga aagggctgct ccatgccgtt ggggagcatg aacccggtcg
     4141 ggaccggctg cggctcgccc tt
//
LOCUS       DASYWM010000334        13328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_506529, whole genome shotgun sequence.
ACCESSION   DASYWM010000334 DASYWM010000000
VERSION     DASYWM010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 13328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13328
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506529"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VGR29_13075"
     CDS             complement(<1..841)
                     /locus_tag="VGR29_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074555.1"
                     /translation="MAVNIGTAPERWLRGVERLVASMANLLPRNATRARRGRRIPAHW
                     VMQRGPARATAPVDALGGFPGSLRSRNALERTTVAVTSRYLVVGEGTNDGFALRMSDL
                     LSADMVRPSLWSNPGLVVSFQDGAVTSTFALNFRGLARGLSGRYRAEEVLRVLQEQGV
                     QRLGLHRLSGGPSLALPWEEVRVHENETLLWAGRARACVGGWYGAVQRSCRVWLTRTS
                     LLWCCSDGEGVNRLSFSDIAGARDGVSDRIRVSFHQGDQQYDLLFDFDVPAPGLVGKE
                     QRTR"
     gene            1197..1736
                     /locus_tag="VGR29_13080"
     CDS             1197..1736
                     /locus_tag="VGR29_13080"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV2074556.1"
                     /translation="MTNHRYGPSDFLDTPVGQVMITVTGAGLAAIDFVPGRSVGDFEA
                     DLVRRGIEPSPDESVLADVLEELTHYFAGELRTFTSQTDLTGISDFTRRVIAATAEIP
                     YGETHTYGEIAQAIGSPGATQAVGNALGANPVPIVIPCHRVIRGDGSMGWYTGGQPIK
                     RTLLGLEGVHFPVQRSLEM"
     gene            complement(1737..2447)
                     /locus_tag="VGR29_13085"
     CDS             complement(1737..2447)
                     /locus_tag="VGR29_13085"
                     /inference="COORDINATES: protein motif:HMM:NF021365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEV2074557.1"
                     /translation="MNARRSANLLMLAARAAVPGETKTRLGRVIGMERAAELYEAFLR
                     DLAARLTRDRGGHDVAWTYSPPEIDFLPWLERIGARSGPGIHVVPQQGQDWGVRQDYL
                     LRWGHEQGYERSVLIATDSPHLERAVIDQAFEGLQSHDIALGRVHDGGYYLIGMAGYW
                     DVLRNVPMSTSSAADSLIGQARSLGRSLIEMPATFDVDEARDLQHLVRALSPDGKDSP
                     ATWAALERLGLRDDVVGS"
     gene            complement(2444..3187)
                     /locus_tag="VGR29_13090"
     CDS             complement(2444..3187)
                     /locus_tag="VGR29_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2074558.1"
                     /translation="MSTQADQVLTAAVIIPCLNEENSIGLLVGDVQEAAADLDLPVRI
                     VSIIVVDNGSDDATAKVAEEAGATVVPEPRRGYGRGCLTGVEAAGEVDVIVLMDGDRS
                     DRPDELPLLLEPLLAGEADLVIGSRMLGTYEPGSMLPQQIVGNKVAAVLLRLLYGVRV
                     TDIGPFRIIRRQTLLGLGMREMTYGWSVEMIARAARAGLAVREVPVTYRNRAGGVSKV
                     SGNLRASLKAGYRIIGAILKSRRGPVGPA"
     gene            complement(3184..4314)
                     /locus_tag="VGR29_13095"
     CDS             complement(3184..4314)
                     /locus_tag="VGR29_13095"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV2074559.1"
                     /translation="MTTALVTGGAGLIGSHIVDAALARGWDVRVLDSLERQTHRAGQP
                     VWVPSEVDFIKGDVRNRADWERALQGVDLVFHEAAYGGYMPEIAKFIHVNSYGTALML
                     ETIRNRNLPVQKVVIASSQAVYNEGAYSCPEHGHFYGETRDIARLEAGDYAMHCPTCD
                     RDAVPVPTDEQAPMGGENVYAISKADQERLVMTFGRATGMPVVALRYSCTYGPRQSLF
                     NPYTGVIAIFCTRLMNDLVPVVYEDGRQSRDLVFVEDVARANVLVAADERANGRVFNV
                     GTGNAVAIGDLASMLSEKLGKQIPPQIQGEFRPGEMRALISDIARIGELGFVPTVDLA
                     IGIDRYLGWIREQGSVRDYFAAAERRLKARQVVKRSRQAAPK"
     gene            complement(4311..5549)
                     /locus_tag="VGR29_13100"
     CDS             complement(4311..5549)
                     /locus_tag="VGR29_13100"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2074560.1"
                     /translation="MSIEQETSLEAQSHERQKHVPNQLTQRTRAVHAGENRDGNTRPL
                     VTPIYQSTAFASDEAGISSERHECGEPSYSRDRFPNLRELEQAVADLECADAGCATSS
                     GMAAISLICLTFLSAGDHVVLAEGSYCDTAELLSEVFGKFGVDVTVVSVAEGGCVESA
                     ITPRTRLLLAETIANPSMTVPDLDALADTAHRHDVLLCVDNTFATPIFCRPIEHGADL
                     VVHSATKFLGGHHDLTAGVVVGFAPLIDRLQTTGYLVGTLPGAMDAWLALRGIRTLAP
                     RMTWIAQTAGVVARALEGHPAIAEVRYPGTASGTKAEIIQRMLPDGAGGMLAIQLAGG
                     DAATETLISALRLIPYVPSLGGVTTSVCYPPRSLEAREQERQANGWLRFSIGLESPED
                     LIADITQALAAIEDESRERG"
     gene            complement(5654..7600)
                     /locus_tag="VGR29_13105"
     CDS             complement(5654..7600)
                     /locus_tag="VGR29_13105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2074561.1"
                     /translation="MREEPSGSDGIEQRSPEAPREAIATWPERSPDRPEPPDQATTPV
                     PLAHPDGPVTALPVTKPRSSRLRSVVRNWPVLIPLALFAIAAMVVPTMTNIATTDDWG
                     YTRSVEILLDDGHLRVFPVVAATAVGQILWGALFGLTFGMTLGVMRLSTVVMVALGAV
                     ALYAILRQLGIGRGRSATGMALYLFNPLGFILSFTFMTDPHFVSILLISLALYMHGLR
                     LGREIGWVIVIASIVAGYAFLIRQQGALIPLSVVLWLIVSRRLPVSRPGIRRALQVAL
                     APALMLLGYYLWLRFFNDVPDVQEGFLQGVIEAGWSGTWLLLQRLPFYVVMYLGVFLM
                     PVIVAILPGFRERLQNRFFESPLGYWLFLGFTGVALTMFFVMTAKGGVMPYMPQFVGP
                     SGFGPPDVQGSRRRIVGEGMLLETLTITAILGAILIGLLVLRRVRGGQQVLRPRRPAT
                     GTPDNHAAGLVAMVAAWQFIGMLPPSYHYLNRGVSLDRYMLPLVPLGIALLLWAVRDI
                     SLAQPVTWLGIALFAVVSTAGTRDYLVFMDAVWDMGRFANERGVENTKLDAGSGWDGY
                     HLYTSMLDEGITKAKSPPGSPWWVYFYAKPTDSTYIVSTVLRAHRGYIMVAHREYDQW
                     LDDEPGDVYLLRRFDAPWPILDPP"
     gene            complement(7713..8585)
                     /locus_tag="VGR29_13110"
     CDS             complement(7713..8585)
                     /locus_tag="VGR29_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2074562.1"
                     /translation="MRTSRERSNEIMRSWYQDAIHQPFALAHGDRNRAGALLVHGFTG
                     SPADMRALGEVVHARGLDVHVMRLPGMASEIDQLNDMTAEIWRDAVLRQWAERRQRYE
                     RTVLVGYSMGGALSLLAAAEESPDLLMLIAPLTRINDRRVPFLPVAKYVTRGVRPYAR
                     LKWDDPRVHEWFDRTRPSMMTRDPEHQQVMEHEAIYSARMLDELRRLLVQTRRSAPKV
                     SSPTIILQGISDRIVLRRDTRELVTRIGGPVTYREMPGDHYLPLPAFDGWSRLCPAID
                     GELRAWMEMNEVTP"
     gene            complement(8596..9585)
                     /locus_tag="VGR29_13115"
     CDS             complement(8596..9585)
                     /locus_tag="VGR29_13115"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEV2074563.1"
                     /translation="MNQDGSTGRPSGEAQELAVFVDFENIRYSTINSFGREPDPLVWR
                     DKALKYGLMSVARAYADFDQHPPQMRTRLDVAGFELHHYPAKRTSDAAGREKIASRSD
                     LNLVIDIINTALVRPDINTFLLFTGDKDFIRLVTTLRNRLGKRVVICGVPGSVSPDLV
                     IAAGEEDPLEMSQSAEVDIAVIRAIDAYIRQLHEGFVPTQSHMSRTLWRFLDRTLLPS
                     EHIEAKVMEFLRKGVLGKRQTINGQGQELVTTELNREHPLVTRALDLPGGDLEIAPKV
                     GEEYEYEDPESTAFTTDRSQDVPDRSNGRGYHRRFAPTNRGLRQRTPVGEAGE"
     gene            complement(10064..11083)
                     /locus_tag="VGR29_13120"
     CDS             complement(10064..11083)
                     /locus_tag="VGR29_13120"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV2074564.1"
                     /translation="MSSPGADAATGDTMANYAVGVDFGGTKVIASVVDVKSGKISGSA
                     KVRTSLFDSGDALMERIYEVVDGALGDAGAKTRDIEGIGVGIAGQVNADEGLLIGTAN
                     LSRSVVNLPMAKMLKKRYDVNAEIRNDVQIAAVGEHHFGAGKGVDNFICVFVGTGIGG
                     AIVVNGELIRGAAGNAGEIGHTTIMADGRICGCGGRGHLEAYASRTAITKTILGELRR
                     GRKSIVTDLIDDLDPTAGHAAIRSGVLKKAVSKKDEVVTEVISDAGRYLGMGLSSVVN
                     FVNPQRIILGGGVIESVDLLFKKTEYWTRHDSLPHAGDQVEIVRSKLGDFSGVVGAAV
                     IAAGA"
     gene            complement(11125..12084)
                     /locus_tag="VGR29_13125"
     CDS             complement(11125..12084)
                     /locus_tag="VGR29_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2074565.1"
                     /translation="MSTSDTDTNVVSEPIWFQSQRTMSLGLMLPVGEASHFGETPRFS
                     DMVEMARVAREVGFEAIWLADHFSFTEAETGDVRGVWECWTMMAAVAAEVPDVQVGSL
                     VACTGFRNPGVIAKMTESIDEISGGRFILGLGAGWHEPEYDQFGFPFDHRVSRFDDAI
                     SIIHPLLREGRADYQGRFFQANGAVNRPRGPRESGAPILVGSSGDRMLGLIARYADAW
                     NTVWHSDSEAVKPLMEKVDAACDEAGRDPKSLIRTAGGNFAMEGYLERRPNAIKGDTD
                     TMAERLNGFRDLGLSHFVCGLDPCTPETIRSFEPVVKKFDATE"
     gene            12882..12998
                     /locus_tag="VGR29_13130"
     CDS             12882..12998
                     /locus_tag="VGR29_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2074566.1"
                     /translation="MMGQDVTSLDGKRGWANESAYCASKFGLTGLTQSLGES"
     gene            13034..>13328
                     /locus_tag="VGR29_13135"
     CDS             13034..>13328
                     /locus_tag="VGR29_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074567.1"
                     /translation="MSSDGGRISERRPKVRGEQSKPLRLSSRGDEHTLSRVPALGFRF
                     RRRPPFYTSLSPPRRSQFSFFALIATVLVYAAVRVLDIQRVVTIDEPVFLSITA"
BASE COUNT         2698 a         4293 c         3920 g         2417 t
ORIGIN      
        1 accgtgtccg ctgctccttc cctaccaggc cgggagcagg gacgtcgaag tcgaatagca
       61 ggtcgtactg ctggtcgccc tgatgaaatg aaacgcggat tcgatcggat acgccatcgc
      121 gcgcgccggc gatatcagag aagcttagcc gattgacccc ctcaccatcg ctacagcacc
      181 agaggagcga tgtcctggtg agccagactc gacaggaacg ctgtacggca ccataccaac
      241 cgccgacaca ggccctggcc cttcccgccc agagcagtgt ctcgttctcg tggacgcgaa
      301 cctcctccca ggggagcgcg agggagggac ctcccgagag ccgatgcaga cccagccgct
      361 gaacgccctg ctcttgcagg acacggagca cctcctctgc ccggtaccgg ccagagagcc
      421 cacgtgccag accgcgaaaa ttcagggcga atgtgctggt gaccgcgccg tcctgaaagc
      481 ttactaccag gccggggttg gaccagagcg agggccgaac catgtctgcc gagagcaaat
      541 cactcatgcg gagcgcaaag ccgtcgttcg ttccctcacc cacgacgagg tagcgagagg
      601 tgacggctac cgttgtgcgc tcaagagcgt tccgcgatct gagggaccca ggaaacccac
      661 ccagggcgtc cacgggcgca gtcgcacgcg cgggaccacg ttgcatgacc caatgggccg
      721 gaatacggcg gccacgccgc gcacgtgtgg cgtttcgtgg cagcagattc gccatgctag
      781 ccacaaggcg ctccacgcct cgcaaccagc gctccggggc cgtcccaata ttgaccgcca
      841 tgctctgcgt tcccttcccc taggtttgac gcggcgccgt caacgccatg aattcctgtc
      901 ccggcaattg tgccaaagag gcaaagaatc aactaccagt cttgtccgta cattccttac
      961 gtgccgatcc ataagccccg gattcatcgc ataatgtatc gggaccgaac atcggggttt
     1021 caaccgcatg aggagatatg gcgcgaatat caaccgtcaa tcggagtgat tgtcagcaac
     1081 ccgtgagaaa ccatctcttc atcccgttga atggcggtca agggcgcgcc acggaatggg
     1141 gaaagcacta tcaacaggtt gacgaggatc gaggacatcg gcttgcagga ttcgaaatga
     1201 cgaaccacag atacgggcca tcagatttcc tggacactcc agtgggccag gtgatgatca
     1261 ccgtcactgg tgcgggtttg gcggcgatcg acttcgtgcc cggcagatcg gtaggggact
     1321 ttgaggcaga cctggtacga cgagggatcg aaccatcccc cgacgaatcg gtacttgccg
     1381 acgtcctgga ggagctaacg cattacttcg cgggggaact cagaaccttc accagtcaga
     1441 cggacctgac gggtatcagc gatttcaccc gccgggtgat cgccgcgacg gcagagattc
     1501 cctatggtga aacccacacg tatggagaaa tcgcgcaggc tatcggtagc ccgggagcca
     1561 ctcaggccgt cggcaacgca ctcggcgcga acccggtgcc gatcgtcatt ccctgtcacc
     1621 gagtgatacg tggcgatggc agcatgggct ggtataccgg tggccagccc atcaaacgca
     1681 cgctccttgg cctggaaggg gtgcacttcc cggtgcaacg gtcgctcgag atgtagttag
     1741 gatccgacaa cgtcgtcccg caggccaagt cgttccagcg ccgcccaggt tgccggggag
     1801 tccttcccgt caggcgacag tgcccggacc aggtgttgga gatcgcgtgc ctcatcgaca
     1861 tcgaacgttg cgggcatctc gatcagggag cgaccgagcg atcgcgcctg cccgatgagg
     1921 gaatcagcgg cggaactggt gctcatcggg acattccgca agacatccca gtaccccgcc
     1981 atcccgatca ggtaataccc accatcatgg acgcgaccga gggcaatatc gtggctctgc
     2041 aggccctcaa aggcttgatc gatcactgcc cgctccagat gcggggagtc tgtggcgatc
     2101 aggaccgagc gctcgtagcc ctgctcgtgt ccccaacgca gaaggtagtc ctgccttaca
     2161 ccccaatctt gcccctgctg ggggaccaca tggattccgg gtccgctcct tgcaccgatg
     2221 cgttcaagcc acggaaggaa atcgatctcc ggcggggagt aggtccaggc tacgtcgtgc
     2281 ccccctcgat cgcgcgtcag gcgagccgcg aggtcgcgca gaaacgcctc gtagagctcc
     2341 gccgcgcgct ccatgccgat gactcggccg aggcgcgtct tcgtttcgcc gggaacggcg
     2401 gcacgcgccg ccagcatcag caggttcgct gatcggcgcg cattcatgcc gggccgaccg
     2461 ggccgcgccg ggacttgagg atagcgccga taatgcgata cccagccttg agcgatgccc
     2521 gcagattacc gctcaccttg gagacaccac ctgcacggtt gcggtacgtc accggcacct
     2581 cgcgcaccgc caggccggca cgcgcggccc tggcgatcat ctcgaccgac cagccgtagg
     2641 tcatctcccg catcccgagg ccaaggagcg tctgccggcg gatgatacgg aacgggccga
     2701 tatcggtcac ccgcacgcca tagaggagcc gaagcagcac agcagccacc ttgttcccga
     2761 cgatctgctg cggcagcatc gatcctggct cataggtgcc aagcatccgt gagccgatta
     2821 ccagatccgc ctcaccggca agcaacggct ccaaaagcaa tggcagctcg tccggccgat
     2881 cgctgcggtc gccatccatg agaacgatca cgtcgacctc accggctgcc tcgactccgg
     2941 tcaggcatcc ccgaccatag ccccgccgtg gctctggtac aactgtcgca cctgcctctt
     3001 ccgccacttt cgcggttgcg tcatcgctac cgttatcgac gacaatgatg ctcacgatcc
     3061 gcaccgggag atccagatcg gcggcagcct cttgcacgtc accgacaagc aagccgatgg
     3121 aattttcctc gttgagacag ggaatgatta cggctgccgt aagcacctga tccgcttgtg
     3181 tgctcatttg ggagcagcct gacgagaccg cttgaccacc tgacgtgcct tcaaccggcg
     3241 ctcggcggcg gcgaaataat cacggacgct cccctgctcc cggatccaac ccagatacct
     3301 gtcgatgccg atagccagat caactgttgg cacaaagcca agctcaccga tgcgagcgat
     3361 gtcgctgatc agcgcgcgca tctcccccgg acggaactct ccctgaatct gcggcggaat
     3421 ctgcttgccg agtttctccg acagcatcga tgccagatca ccgatggcga cggcattgcc
     3481 cgtaccgaca ttgaagacgc gtccgtttgc ccgttcgtcc gccgcaacga gcacgttcgc
     3541 ccgggcgaca tcctccacaa agacgagatc ccgggattgc cgcccatcct catagacgac
     3601 cggcaccaga tcgttcatca ggcgcgtgca gaagattgcg atgacacccg tatagggatt
     3661 gaaaagcgac tgtcgcggtc cataggtgca ggagtacctg agcgccacga ctggcatccc
     3721 ggtggcgcga ccaaaggtca tcacgaggcg ttcctgatcc gcctttgaga tggcgtagac
     3781 gttctcgcca cccattggcg cctgctcatc agtggggact ggtacggcat cccgatcgca
     3841 ggtgggacag tgcatcgcat aatcgcccgc ttcgagccgg gcgatgtccc gcgtctcgcc
     3901 atagaaatga ccatgctccg ggcaggaata cgcgccctcg ttgtagaccg cctgggacga
     3961 ggcaatgacg actttctgga caggcaggtt gcgattgcgg atcgtctcca gcatcagcgc
     4021 ggtaccgtag gagttgacat ggatgaactt ggcgatctcc ggcatgtagc cgccgtaggc
     4081 tgcctcgtga aagacgagat caacgccttg cagggcccgc tcccagtccg cgcgattgcg
     4141 gacatcgccc ttgatgaaat cgacctcgga cggtacccac accggttgac ccgctcgatg
     4201 cgtctgccgc tccaggctat caagcacccg cacatcccac ccccgagcga gggcagcgtc
     4261 aacgatgtga gagccgatca ggccggctcc tccggtgacc aatgcggtcg tcatcctctt
     4321 tccctactct cgtcttcgat agcggcaagt gcctgcgtga tgtcggcaat caggtcttct
     4381 ggtgactcca gaccaattga gaaccggagc cacccgttcg cctgcctctc ttgctctcgt
     4441 gcctccagcg agcgtggcgg ataacagacg gacgttgtca cgccaccgag gcttgggaca
     4501 taggggatca aacgcagggc cgaaatgagc gtctcggtag ccgcatcgcc cccggcaagc
     4561 tggatggcca gcatcccacc ggccccatcc ggcagcatac gctggatgat ctccgctttt
     4621 gttccggacg cggttcccgg atatcggacc tcggcaatcg ccgggtggcc ttccagcgca
     4681 cgtgccacca ctcctgccgt ctgggcaatc catgtcatgc gcggcgcgag cgtgcggatg
     4741 ccccgaagcg ccagccatgc atccatcgct ccgggcaatg tcccgaccag ataccccgtc
     4801 gtctgcaacc gatcgattag cggggcaaaa ccaacgacca cacctgcggt cagatcgtga
     4861 tgtccgccaa ggaatttcgt cgcgctgtgg acgaccaaat ccgcgccgtg ttcgatcggg
     4921 cgacagaaga tcggggtggc gaaggtgttg tcgacgcaaa ggagaacatc atgacgatgg
     4981 gcggtatctg ccaaggcatc gagatcgggt acggtcatcg aggggttggc gatcgtctcg
     5041 gcgagcagga gccgggtccg cggcgtgatt gcactctcca cgcaaccgcc ctcagccaca
     5101 ctgacgacgg tgacatcgac cccaaatttc ccaaacacct cgctcagcag ttcggcggta
     5161 tcgcagtagg atccttcggc aagcaccacg tgatcaccag cagagagaaa ggtgagacag
     5221 atgagggaga tcgccgccat cccggaagag gttgcgcagc ccgcatcagc acattccaga
     5281 tcagcgaccg cctgctccag ctcacgcagg tttgggaacc gatcccggct gtagctcggc
     5341 tcaccgcatt cgtgtctctc cgacgagatt ccggcctcat cagaggcgaa cgcggtgctc
     5401 tggtagatgg gtgttacgag ggggcgagtg ttgccatccc ggttctcccc ggcatggacg
     5461 gcgcgcgtcc gttgcgtcaa ctggtttggc acgtgtttct gccgttcatg gctctgcgcc
     5521 tccagcgacg tttcctgctc tatggacacc catattcctt cctgcagaca tccctcggga
     5581 tcgcctgaga cgcgtccggt atgccgggct agtccgatgt agacgaatat tgcaaaattc
     5641 gggcccctgt ctgtcaggga ggatcgagga ttggccaggg agcatcgaac cggcgcagga
     5701 ggtagacatc gcctggttca tcgtccagcc attggtcgta ttcgcggtgc gcaaccatga
     5761 tgtagccgcg gtgggcacgg agaacggttg aaacgatgta ggttgaatcg gtcggcttgg
     5821 cataaaaata gacccaccat ggtgagccgg gaggcgactt ggccttggtg atgccctcat
     5881 ccagcatgga ggtatagagg tggtatccat cccagcccga gccagcatcc agcttcgtgt
     5941 tctcgacacc ccgctcgttc gcgaagcgac ccatgtccca gacagcatcc atgaaaacaa
     6001 gataatcgcg ggtgccggcc gtagagacga ccgcaaacag ggcgataccc agccaggtca
     6061 ccggctgcgc cagcgaaatg tcccgcaccg cccataagag aagtgcaatg cccagtggga
     6121 ccagcggcaa catgtatcga tccagtgaaa caccgcgatt gaggtagtga tacgagggcg
     6181 gtagcatgcc gatgaactgc catgccgcca ccatggcgac cagaccagcc gcatggttgt
     6241 caggggtgcc tgtggcggga cgccgtggtc gcaagacctg ctgtccacct ctcacgcgcc
     6301 gcagcaccag gagcccgatc aggatcgcgc ctaggattgc cgtgatggtc agtgtttcga
     6361 ggagcatccc ttcgccgacg atcctgcgcc gggagccctg cacatcaggc gggccgaagc
     6421 cggaggggcc gacgaactgc ggcatatagg gcatgacgcc cccttttgca gtcatcacga
     6481 agaacatcgt cagcgcgacg ccggtaaagc caaggaaaag ccagtacccc aatggtgatt
     6541 cgaagaaccg gttctgaagc cgctcacgaa acccggggag gatcgcgacg ataacaggca
     6601 tgaggaaaac gccaagatac atcaccacgt agaagggcag ccgctggagc agcaaccatg
     6661 ttcccgacca cccggcctca atcactccct ggagaaagcc ctcctggaca tccggtacat
     6721 cgttgaagaa acgcaaccac aggtagtatc cgaggagcat cagggccggt gccagtgcaa
     6781 cctggagcgc gcgcctgata ccaggccggc tcaccgggag gcgcctactg acaattaacc
     6841 agagcacaac cgagagcgga atgagcgccc cttgctggcg aatcaggaag gcataccccg
     6901 ccacgatgga cgcaatcacg atcacccagc cgatctcgcg cccgagccgc aatccgtgca
     6961 tgtagagcgc gagcgagatg agcaagattg aaacgaaatg cgggtccgtc atgaatgtaa
     7021 aggaaagaat gaaaccgagg ggattgaaga ggtagagggc cataccggtt gcgctgcggc
     7081 ccctcccgat acccagctgg cgcaggatcg catagagggc aaccgctccc aaggcaacca
     7141 tcaccacggt ggagaggcgc atcacgccaa gcgtcatgcc gaaggtgagc ccgaagagtg
     7201 ctccccagag gatctggccc accgccgtcg cggccacgac cgggaagaca cgcaagtgcc
     7261 cgtcgtccag caggatctcc accgaccgtg tgtagcccca gtcgtcggtg gtggcgatgt
     7321 tcgtcatggt ggggacgacc atcgccgcga tggcaaagag ggccagggga atcaggaccg
     7381 gccagtttcg gacgacggag cgcagacgag aggaccgggg ctttgtcacc ggcagggcag
     7441 tgaccggacc atcagggtgc gccaagggga ctggtgtcgt cgcctggtca ggtggctccg
     7501 gccgatccgg tgatcgctcg ggccaggtcg cgatcgcctc ccgaggtgct tccggcgacc
     7561 gttgctcgat tccgtcggag cccgatggtt cttctctcac gcacctgccc tcgtatcgtc
     7621 attgtgtgtc gtggcgcctg cccgcacatc ggcactctgg tcgatctggg gagcccgcat
     7681 gaggattcca tggtgctgcc agagggccga acctacggtg ttacctcgtt catctccatc
     7741 catgcacgaa gctcaccatc gattgccggg cacagccgcg accacccatc gaaggccggc
     7801 aacggcaggt aatggtcgcc aggcatctcg cggtacgtca ccggcccccc gatccgcgtg
     7861 accaattcgc gggtatcgcg gcgcaggaca atgcggtcgc tgattccctg caggatgatg
     7921 gtgggcgagc tcacctttgg ggctgaacga cgggtttgca ccaacagccg cctcaactcg
     7981 tcaagcatac gtgcggaata gatggcctca tgttccatca cctgctgatg ttcggggtca
     8041 cgcgtcatca tcgaagggcg ggtacggtcg aaccactcgt gcacccgagg gtcgtcccac
     8101 ttcaatctgg cgtagggccg aaccccccgc gtcacatact tggccaccgg caagaagggg
     8161 actcgtcgat cgttgatccg cgtcagcggt gcgataagca tcaacaggtc aggcgattcc
     8221 tcggcggcgg caagcagcga gagcgctccc cccatcgagt agcccacaag caccgttcgc
     8281 tcgtatcgct gccggcgctc tgcccattgc cggaggaccg cgtcgcgcca gatctccgca
     8341 gtcatatcat tgagctggtc gatctccgat gccatcccgg ggagccgcat gacatggacg
     8401 tcgagcccac gggcatgcac cacctcgccg agggcgcgca tatccgccgg tgagccggtg
     8461 aacccgtgaa ccagcagggc accagctctg ttccggtcac catgagccag ggcaaagggc
     8521 tggtggatcg catcctggta ccaggagcgc attatctcgt tgcttcgttc gcgtgatgtc
     8581 cgcaaggaaa gccctctact cgccggcttc gccgaccggc gtgcgctgcc gaaggccacg
     8641 gttggtcggg gcgaaccttc ggtggtaccc gcggccattc gaccgatcag gaacatcctg
     8701 gctgcgatcc gtcgtgaacg cggtgctctc cggatcctcg tactcgtact cctcgccaac
     8761 cttgggagcg atctccagat cgcccccggg aagatccagc gcgcgggtca caagcgggtg
     8821 ttcccgatta agctcggtag tgacgagttc ctgcccctga ccattgatcg tctggcgctt
     8881 gccaagcaca cccttgcgca ggaactccat cacctttgcc tcgatgtgct ccgagggcag
     8941 caacgtgcga tccaggaacc gccacagcgt cctgctcatg tgactctgcg tcggaacgaa
     9001 accctcgtgc aactggcgga tataggcatc gatcgcccgg atcacggcga tatcgacctc
     9061 cgccgactgc gacatctcca ggggatcctc ttctccggcg gcgatcacca aatccgggct
     9121 caccgacccc ggcacgccac agatgacgac ccgcttgccg agccggttcc tgagcgtggt
     9181 gacgaggcgg atgaaatcct tgtcgccggt aaagagcaaa aaggtgttga tgtctggccg
     9241 caccaatgcg gtattgatga tatcgatcac gagattgagg tcagagcggg aggcgatctt
     9301 ctcacggccc gccgcgtccg aagtccgctt ggcgggatag tgatgcagct caaaaccggc
     9361 cacatccagc cgggtacgca tctggggtgg gtgctgatcg aagtccgcat aggcacgtgc
     9421 cacagacatc aggccgtatt tcagcgcctt gtcacgccac accagtggat ccggctcccg
     9481 tccgaaggag ttgatcgtcg aataccggat gttctcaaaa tcgacaaaga ccgcgagttc
     9541 ttgggcttca ccggacgggc gtccagtgga tccatcctga ttcatccata tcctccaaaa
     9601 ccaaatatct gtgcatggcc cttgtacatc cggagatctc attcttacct ggttcgggag
     9661 cgtcgcaccg tacctgtccc cggtcgtatc gcgaggcacc attgatccct gaaaaccgca
     9721 tatcgatcaa gagattgtgc acattgccgt ggggaggcaa cgctatttca ctccccaccg
     9781 cttccggact caatacatct gcttcccggg cgtgccagca tcttaattgc ggagtgtacc
     9841 gcacctgcaa atgcacctgt acctgcatgg caagggagaa gcaaagcgct tcccctgttc
     9901 ctggacgggg cgatattatt gcaccatggt gggttaaagc gttcaacaac cttgctgttg
     9961 cgggctacag agagcgtcat gctgacagaa gcgaaaacgc tgtgcgacgt atcccggcaa
    10021 caacaatgtc cgaaacccgc cacgccggag cctgcccggc ggcctatgcc cctgcggcaa
    10081 tgaccgccgc gccgaccaca ccggagaagt cacccagctt cgatcgtacg atctccacct
    10141 gatcacccgc gtgcgggagc gaatcgtgac gcgtccaata ctccgtcttc ttgaaaagca
    10201 gatcgaccga ctcaatcaca ccgccgccaa gaatgatacg ctggggattc acgaagttca
    10261 cgacggatga gagccccatg ccgagatacc tgccggcatc cgagatcacc tcggtcacga
    10321 cctcatcttt tttggaaacc gccttcttga gaactcccga gcggattgcc gcgtggcccg
    10381 ccgtcggatc aagatcatca atcaggtcgg tcacgatcga cttgcggcca cgacgaagct
    10441 cccccaggat cgtcttcgtg atcgccgttc gggacgcata cgcctcaaga tgtccacgtc
    10501 caccgcagcc gcagatgcgg ccatcggcca tgatggtggt atggccgatc tcgccagcgt
    10561 tcccggcggc gccacgaatg agttcgccgt tgacgacgat cgcgccaccg atcccggtgc
    10621 ccacgaaaac acagatgaag ttatcgacgc ccttgccggc gccgaagtga tgctcgccga
    10681 ccgcggcgat ctggacgtca ttgcggatct cggcgttgac gtcgtaccgc ttcttcagca
    10741 tcttcgccat cggcaggttg acgaccgacc ggctcaagtt ggccgtcccg atcagcaagc
    10801 cttcgtcggc attgacctgt ccggcaatac caacgccaat gccctctata tcccttgttt
    10861 tcgccccggc atcacccagg gcgccgtcga ccacttcata gatgcgctcc atcaaagcgt
    10921 caccggaatc gaagaggctg gttcggacct tcgctgaacc gctaattttc ccgctcttga
    10981 cgtccacgac cgaggcaatc accttggtgc cgccgaagtc cacgcctact gcataattcg
    11041 ccatagtgtc cccagtagca gcatcggcgc caggggagct caccaggaac tcccacacgg
    11101 gccgagccga acgaaactct tccactattc agtcgcatca aacttcttga cgactggctc
    11161 gaatgaacgg attgtctccg gagtgcacgg gtcaagccca cacacaaaat gggatagtcc
    11221 gagatcgcgg aagccgttca atcgctccgc catcgtgtcg gtatctccct tgatcgcgtt
    11281 cgggcgacgc tccagatagc cttccatcgc gaagttgccg ccagctgtcc ggatgagcga
    11341 cttcggatca cgcccggcct catcgcaggc ggcatcgacc ttttccatga gcggtttcac
    11401 cgcctccgag tcgctgtgcc agacggtgtt ccaggcgtcg gcgtatcgcg cgatcagacc
    11461 aagcatgcgg tctcccgagg agccgacaag aatcggcgca ccgctctcgc gcggaccacg
    11521 gggccggttg accgccccgt tggcctggaa gaagcgcccc tggtagtcgg cccttccctc
    11581 ccgcaggagg ggatggatga tgctgatcgc atcgtcgaag cggctgaccc tatgatcgaa
    11641 cgggaacccg aactgatcgt attcgggctc atgccagccg gcgccaagac cgagaataaa
    11701 gcgtccaccg ctgatctcgt cgatcgactc ggtcatcttg gcaatgacgc ccggattgcg
    11761 gaacccggtg caggccacca acgatccgac ctggacatcc gggacctcgg ccgcgacggc
    11821 agccatcatc gtccagcact cccagacgcc gcgaacgtct ccggtttcag cctctgtgaa
    11881 cgagaagtga tccgccagcc agatcgcctc gaagccgacc tcgcgcgcga cccgtgccat
    11941 ctccaccatg tcggagaacc gtggggtttc cccaaaatga gatgcctctc cgacgggaag
    12001 catcaaccca aggctcatcg tacgttgtga ttggaaccag attggttcgg aaaccacatt
    12061 ggtatcggtg tcagacgtgc tcatgctctc tcctggtgcg tggcgaacac atttcctgcg
    12121 tcaacctacc gcacgccgga tgccctgccc agtggcctct cgcatctgga ggccaaacac
    12181 agcgacatgt ggcgccctag gcaaccccgg ccccgcccgc cgtgcggtgc ggaggcagat
    12241 cagccggggt gaccaaaacg atcatgatca cgatcattcg cacgcggcgt atcgatcatg
    12301 ttgggacggc accggatgcg ggagatcggt gaacgcgctc tggcggtcgg tcacatgcgt
    12361 gctccgcgct ctcacgaccg cccctactgt taatacgcgt cgagggggcc agctgcgcca
    12421 gggcccgggc actcaccgag ggcgtcagcg tcgcagtgcg agacagacgg atgcccagcc
    12481 acgtcgatga tggcggcaag acgaccgccc cttctgctgg cggatacata cgggcggcga
    12541 gagagccgtt tcccgggagt gtagggaatg gcatggggac tctcaatcga ttgtggggat
    12601 atatcgcgca cccctttcca tgcccgcctt gggtccgact cgttcccgcc atactcatgg
    12661 gcgcatccat ctgtgcccaa gtcacgttgt ccagccaccc cttggtggct ggcaaacctg
    12721 gcatagcggg tctttatgtg tagccgcggt ccgatcgagg cggccagtgt gctgctgttc
    12781 cgcgcttctc gtcatgccaa cgccaatcaa tgtcgggggt ggcccattca ccgcgaccgc
    12841 atttcaccac agggacagcg cattgtgtga gaggcatggc gatgatgggt caggatgtca
    12901 cgagtttgga cggcaagcgt gggtgggcca acgagagcgc ctactgcgcc tcaaagttcg
    12961 ggctgaccgg gttgacgcaa tccctgggtg agagctagcc tgtcgtcaaa cgacgttgcc
    13021 ctgggaattc gatatgagta gtgatggcgg gcgtatcagc gagcgacgtc ccaaggtgcg
    13081 tggtgagcag agtaaaccgc ttcgtctctc cagtcgtgga gatgaacaca cactgtcacg
    13141 ggtccccgca ctcggctttc ggtttaggcg tcgtcccccc ttctacacgt cgctctctcc
    13201 ccctcgccgc tctcagttct cgtttttcgc cctgatcgct accgtgctgg tctatgccgc
    13261 ggttcgagtg ctcgatatcc agcgtgttgt cacaatcgat gaacctgttt ttctttcgat
    13321 caccgcca
//
LOCUS       DASYWM010000335         9065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_300071, whole genome shotgun sequence.
ACCESSION   DASYWM010000335 DASYWM010000000
VERSION     DASYWM010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300071"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VGR29_13140"
     CDS             <1..844
                     /locus_tag="VGR29_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074568.1"
                     /translation="QNVGGRTGDWFPGSLDAVGREVMVHHRRAPIDLMLDVHFEGDPG
                     STKGVFLIMPDGDGLRTVTPYTGSDALAANTLDHSMAREIARAVSRSTGLLMRQRGVI
                     EPGVMSERATHVGAGLGWRLAMFGYTAPARERMVRLVLECGNIEADASIIDRPGFAHQ
                     VAGGVMEGVARAMGMGPVQAPVYPGFGTTGTLREPRLTTVTLDAVTVNARQWAELDAA
                     IRGVWKAGRRFWTRGWAIGEAVEGNPVWWITGKGLKSDLQWRVWSGGTDLAGMDVLGL
                     PCVK"
     gene            complement(1091..1471)
                     /locus_tag="VGR29_13145"
     CDS             complement(1091..1471)
                     /locus_tag="VGR29_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074569.1"
                     /translation="MPRHEKFEYSAPPPSPGATPAQSIIMGFVGGVLALVTMTGVQKS
                     ASSAIQVGSFLVALGLLFGIGMVSKSRTVQMIVAMLLIFAGGITIGLGANVGSWLWYV
                     GAAFFFLCAIVVLIKDQIVERSRR"
     gene            complement(1533..2129)
                     /locus_tag="VGR29_13150"
     CDS             complement(1533..2129)
                     /locus_tag="VGR29_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2074570.1"
                     /translation="MKLILKRPFALLLLCVAMVIGAAGITASAQDGATATPAAAVTRE
                     VINEGHPDAAPGQVLQLVRYTIPPNMLLPVHIHPGIQVVVVESGTLHYTVVEGSVPIT
                     RAAAEGTPALTEQLTSGQETDFGPGDRFVEPAGMVHFGENRTGKPVVLLVASLFEADA
                     PPSTLVEVSPVATPPVAEEAFCRSGVVTTYTDRASLPH"
     gene            2518..2796
                     /locus_tag="VGR29_13155"
     CDS             2518..2796
                     /locus_tag="VGR29_13155"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEV2074571.1"
                     /translation="MLATWIGQGIKTARKTMEDAEGVRKLAEQFGGKKDLLLWTQGRY
                     DLVGVMDIPSDEALAVLALQIGASGATRTETLRGFTADEMSGILERLG"
     gene            2927..4051
                     /locus_tag="VGR29_13160"
     CDS             2927..4051
                     /locus_tag="VGR29_13160"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV2074572.1"
                     /translation="MDRAEIPAWLTTSEVTVPLRRKFGESAVSVAGWSCHLLIGGSSQ
                     ILGVWRVAGNARIGDTLQPCSMILKGWPAPEGGMLPSALDWPHRERDLYSSGLLAHLP
                     GGISAPDHYGEVCLPDGSLWIWLEDVTDESDGVWPVDRYATLARRMGRFSGAYLTGCP
                     FPDSDGLSQRWLRQRVEAAAPAMATFKQSIHHPQVERVYSPHVVDTFMRIWARRDAYC
                     AYLEGFPQTFCHMDAFRRNVFIRKEPDGSEDMLLIDWAFAGKGAVSEELTSLIAASVI
                     LMEVPVEEARYLEAEAIESYISGLGDAGWRGDPEMVRTGYGVAAALRYGVGALRLSMS
                     ILLEERYHPYVEQVLKHPMDEILANMAAVHAWMADLVPDA"
     gene            4171..4596
                     /locus_tag="VGR29_13165"
     CDS             4171..4596
                     /locus_tag="VGR29_13165"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEV2074573.1"
                     /translation="MTMNPLVGTWRLLSFEVRDERGEITYPFGRDVLGFITYTADGHM
                     SVQFGRKDRPHLGADDWWGATPAEISAAARDYFAYCGTYVYQDGEVVHRIGLSLMPNW
                     IDGTQLRLVQLDRDTATLSTHPMPVCGQLQVATLVWQRV"
     gene            4887..5216
                     /locus_tag="VGR29_13170"
     CDS             4887..5216
                     /locus_tag="VGR29_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074574.1"
                     /translation="MFRSIRLLIVLALAIASLLLGSLQFAFADPDVSDAPVPLHRHYI
                     VTSNGDRVEVGPRLCDDLDNPALRSAFNQFHSNLHIALPNSPGPAQSAPGLHDGRGAE
                     ITGVPGC"
     gene            5445..5633
                     /locus_tag="VGR29_13175"
     CDS             5445..5633
                     /locus_tag="VGR29_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074575.1"
                     /translation="MRRSVVSLAFVLTMVLGAVPGPARVAVAAPAEPAVCVAETITGV
                     ISTAGGRFLATAIVVVDA"
     gene            5903..6670
                     /locus_tag="VGR29_13180"
     CDS             5903..6670
                     /locus_tag="VGR29_13180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2074576.1"
                     /translation="MTDQHGASLNGKVAIVTGASSGLGRATAMALAQHGAHVALIARS
                     GAGLQDIAQEIASGSGSRALPLPLDLADETALVGAVARTVADLGRVDILINAAGTDKP
                     GPAETLTTEDWDQVLAVNLRAPFILAREVFPHMRQAGGGTIVNVSSVAGKRGWANASA
                     YCASKFGLTGLTQSLAAEGKLHGIRASILYPGGMATGWGKWSAAERDDALEEPPPPTK
                     ALPPDEVAALIVWMVTAPAELVLNEAVVSPLEEEGWP"
     gene            complement(7138..7314)
                     /locus_tag="VGR29_13185"
     CDS             complement(7138..7314)
                     /locus_tag="VGR29_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074577.1"
                     /translation="MVFLLLLALQLKTVMLPHSHSTPIDGGVNARAAIPELPGDVDRR
                     PFMAVSVPAVDIAD"
     gene            7313..7717
                     /locus_tag="VGR29_13190"
     CDS             7313..7717
                     /locus_tag="VGR29_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV2074578.1"
                     /translation="MAQESAKAATELVAEAKSRTENLTPDDVTREMAEADAVLIDLRE
                     PQERAQNGTIPGAIHAPRGMLEFYADPANSYHRPEFDPNRRTILHCASGGRSALAADT
                     LKQLGYSNVAHLDGGINAWKETGHEVEEGGSS"
     gene            complement(7960..8439)
                     /locus_tag="VGR29_13195"
     CDS             complement(7960..8439)
                     /locus_tag="VGR29_13195"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2074579.1"
                     /translation="MTPGTEPFVLGPDESRGIGGGPWRTRVRGAETGGLLIIGDATMP
                     PMSAGPSLHIHTHEDEASYVVEGVLTVVLGSERFDVPAGGIAWLPRGVPHTFANLTND
                     PVRVVGVAVPAGLEGMWAEFAAYFASLQGPPDEAALDEIGKRYGITVIGPPIEIPER"
     gene            complement(8763..>9065)
                     /locus_tag="VGR29_13200"
     CDS             complement(8763..>9065)
                     /locus_tag="VGR29_13200"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2074580.1"
                     /translation="EVKTPLKESRMNDMVPGGPRWHGAHHIALTTRDMDATVRFYHQL
                     LGMPLVGSVGANPIHGRHYMFDAGGFLIGFFEHPDHAMSAVPAGWSRAFAFLPGAF"
BASE COUNT         1621 a         2776 c         2976 g         1692 t
ORIGIN      
        1 ccagaatgtg ggtggccgca ccggggactg gtttcccggt tcgcttgatg ccgtcgggcg
       61 ggaggtaatg gtccatcacc gacgggcacc aatcgacctg atgctggatg tccactttga
      121 gggagacccc ggcagcacga aaggcgtctt cctgatcatg ccggacggcg atggcctccg
      181 taccgtcacc ccgtacactg gctccgatgc cctggcggcg aatacgctcg accacagcat
      241 ggcgcgggag attgcccggg cagtctcccg tagcaccggg ctcttgatgc gccagcgtgg
      301 cgtcatcgag ccgggcgtga tgagcgagcg ggcgacccac gtcggcgcgg gcctcggctg
      361 gcggttggcg atgttcggct ataccgctcc agcccgggag cggatggtcc gcctcgttct
      421 cgagtgcggc aacatcgagg cggatgcatc gatcatcgat cggcctgggt tcgcccacca
      481 ggtggctggg ggcgtcatgg agggcgtcgc gcgagcaatg gggatggggc cggtgcaggc
      541 tccggtgtac ccggggttcg gaacgaccgg cacgctgcgg gagccgcgac tgacgacagt
      601 gaccctggat gcggtcacgg tcaatgcccg gcaatgggca gagctggatg cggcgatccg
      661 tggcgtctgg aaggcgggtc gacggttctg gacgcgtggt tgggcgatcg gggaggcggt
      721 ggaggggaac ccggtctggt ggatcaccgg caaggggctg aagtcggatc tccagtggcg
      781 ggtctggtcg ggcgggacgg acctcgcggg gatggatgtg ctgggacttc cctgcgtgaa
      841 gtaaggatgc gaatcggttg gggctggggt agtggttcgg caccggcttc gcccgggagt
      901 cttcatatat aaacaatgcc gcaggcatta atcggccgcg tccgctcgac gcctgcggca
      961 ttgcctacgc gcgagagtgg tcccttgccg cagcagcgtc gtgccgtgcg ccgtgtcagg
     1021 aggacattgc tgcggcaagt cctccgctac agatagcgac atctctgaaa atagctccag
     1081 ggtggctcga ttatcggcgg gagcgctcga cgatctggtc cttgatcagg acgacgatgg
     1141 cgcagaggaa gaagaaggct gcaccaacat accagagcca tgagccgacg ttggctccga
     1201 gcccgatcgt gatgcctccc gcgaagatga gcagcatggc gacgatcatc tggaccgttc
     1261 gtgacttcga caccatgccg ataccgaaga ggagaccgag tgcgacgagg aagctgccga
     1321 cctggatcgc actgctggcg ctcttctgca cgccggtcat ggtcacgagc gcgaggacgc
     1381 cgccgacaaa gcccatgatg atggattggg cgggcgtcgc gccgggcgat ggcggtggag
     1441 cggaatattc gaatttctcg tggcgaggca tggtgtgcgc ggactccaga tgatgtacgt
     1501 caacccggga caagtgtaat ctggcacgtt cctcaatggg gaagggatgc acggtcggtg
     1561 tatgttgtga caacccccga gcggcaaaag gcttcctcag ccaccggcgg ggtggcaacg
     1621 ggggacacct cgacaagggt gctgggcggc gcatcggcct cgaagagtga ggcgacaagg
     1681 aggacaactg gcttgccggt ccggttctcg ccgaaatgca ccatccccgc aggctccacg
     1741 aaccggtcgc cggggccgaa atccgtctct tggccgcttg tcagctgttc cgtcagggcg
     1801 ggcgtgccct cagccgcggc gcgggtgatc gggacgcttc cctcaacgac cgtgtagtgc
     1861 agggtgcctg actcaaccac cacaacctgt atgccgggat ggatatgcac cggcagcagc
     1921 atgttcggtg ggatggtata gcgcacgagc tggaggacct gccccggcgc ggcatccgga
     1981 tggccttcgt tgatcacttc gcgggtcacc gcggcggcag gtgttgcggt ggcaccgtcc
     2041 tgtgcgctcg ccgtgatccc ggccgcgccg atcaccatgg ccacacagag caggagcagt
     2101 gcgaagggac gcttcaggat caacttcata ccggtgtcct cctgacacta atgcgcctgc
     2161 cgcagcatct gtgtttattg tgagacccgg cctcacgcta tgcgaagagg cctcccgctg
     2221 caatccaccc gactccggca tccggcctgc agaccatacg gcccttcccg ctgatgcggt
     2281 ctgtcgttgt gctcccttgt tcgggaggcg cgaggccgca atgggcggca tgcattcaat
     2341 gcctacggcc ttgcctcctg gtccgtgcga atcgtcctgg ccactgtcct ccctgttgtc
     2401 ggcagagcgc gccggcagac gtgtgttgac tcctcccgcc tcgggagtat catgaagttg
     2461 ggccccagcc taaacatact gcgtcatcaa ggagcgggag cagtgccata gtacctggtg
     2521 cttgcaacct ggatcggcca aggcatcaag accgcccgaa agacgatgga agatgccgaa
     2581 ggggtgcgca aacttgccga gcaattcggg ggcaagaagg acctgctgct gtggacacag
     2641 ggacgctatg acctcgtagg ggtgatggat atacccagcg atgaggccct tgccgtcctc
     2701 gcgcttcaga tcggagcatc tggagcaacc cggaccgaga ccctgcgcgg tttcaccgcc
     2761 gacgagatga gtggcattct ggaacgcctc ggctgagcgc gcgaccagtg ccaagccagg
     2821 tgcctgatcc tcacgagggc tgcttgagcc tgatgcggtc actgcttcgt ttgctgcgcc
     2881 cgccatgtga ccatgccgcc gtagtgggca caggaaggag aaggggatgg acagagccga
     2941 aatcccggca tggctgacga cctccgaggt gaccgtccct ctccgccgga aattcggtga
     3001 gtcagccgtc tccgtcgcag gctggtcgtg ccatctcctt atcggcggca gcagccagat
     3061 actgggtgtg tggcgtgtgg caggcaacgc caggatcggc gacacgctcc agccctgctc
     3121 gatgatcctc aaggggtggc ccgcaccgga ggggggcatg ttgccctccg ccctggattg
     3181 gccgcaccgg gaacgggacc tgtacagctc cgggctgctt gcacatctgc caggcgggat
     3241 ctcggcgccg gaccattacg gagaggtatg tctgcctgat gggtcactgt ggatctggct
     3301 tgaggacgtt acggacgaga gcgacggtgt ctggcccgtt gatcggtacg cgacgcttgc
     3361 tcgtcgtatg ggccggttca gcggtgccta cctgactggc tgtccttttc ccgattctga
     3421 cggcctgagc cagcggtggc ttcggcagcg ggtcgaggcg gccgcgccag cgatggcaac
     3481 gttcaagcaa tcgatacacc acccgcaggt cgagcgtgtc tactcgccgc atgtcgtcga
     3541 cacattcatg cgcatctggg ccaggcgtga cgcctattgt gcctatctgg aggggtttcc
     3601 ccagacgttt tgccacatgg atgcattccg gcgcaacgtc ttcatccgga aagagccgga
     3661 cgggagcgag gacatgctcc tgatcgattg ggcattcgcc ggcaaagggg cagtcagcga
     3721 ggagttgaca tccctgattg cggcgagcgt aatcctcatg gaagtgcctg tggaggaggc
     3781 ccggtacctc gaagcagagg cgattgagag ctacatctcg ggcttggggg acgcaggctg
     3841 gcgcggggat cctgagatgg ttcggaccgg gtatggcgtt gccgctgcgc tgcgctacgg
     3901 tgtcggggca ctgcgcctct cgatgtctat cttgctcgag gaacggtatc atccctatgt
     3961 cgagcaggtg ctgaaacacc ctatggacga gatccttgcg aacatggcgg cagtgcatgc
     4021 atggatggcg gacctggttc ccgacgcgtg atgcctgtgc cgcgtggtcg caatgcgtct
     4081 gcctccgccg ggccccgctt catgcgtgaa ggtactgcag gacggcttag ggaacgcttc
     4141 tgataacgcc gcatgccagg gagatggccg atgacgatga atccgctggt cggcacctgg
     4201 aggcttctgt ccttcgaagt ccgggacgaa cgtggagaga tcacctatcc cttcgggcga
     4261 gacgtgctcg gcttcatcac ctataccgcg gatgggcaca tgtcggtgca gttcggacga
     4321 aaggaccggc cgcacctcgg cgccgatgac tggtgggggg caacgcccgc ggagatttcc
     4381 gccgcggccc gcgactactt cgcgtactgt ggcacgtatg tgtaccagga tggggaagtt
     4441 gtgcaccgga tcggattgag cctgatgccg aactggatcg atgggacgca gcttcgcctg
     4501 gtccaactgg accgcgatac ggccaccctc tcgacgcacc ccatgccggt gtgcggccag
     4561 ttgcaagtcg ccacgctcgt ctggcaacgg gtctgaagct cctggtgtcg caccgaccag
     4621 agatgtgtgc tgacacctgt aatctcaatg ggcggtacca ccccctgcgc agccggtggg
     4681 gagattggtt atcgccggca actgcacgaa ttacctacat gctcccgcgc caattaatga
     4741 gtatttctac cgatgccctc ttccttccgc tcgtccagac tggcggtatg ggattccaca
     4801 tcccgacacc tgtccttact gggcgtttgt gtaacgcatc tatccgggga caccggagat
     4861 gcgacatcgc gaaagggagg ctcttcatgt tccgctctat ccgcctgctc atcgtccttg
     4921 ccctggccat cgcctcgttg ctgctcggca gccttcagtt cgcattcgcc gatccggacg
     4981 tgagtgatgc cccggttcct cttcaccggc actatatcgt gacgtctaat ggtgacaggg
     5041 tcgaggtggg cccccggctc tgtgacgacc tggataatcc ggcgttgcgg tctgcattca
     5101 accagttcca ctcgaacctg cacatcgctt tgcccaactc tccggggcca gcacaaagtg
     5161 cacctggctt acacgatggc cgcggtgcgg agattacagg ggttcccggt tgctagtggg
     5221 cccggacgtc agtctccaag aaacctgcac ccgtcgcggt ggcttgggcg tctcatcagg
     5281 ccacagaggc agtcgacacg gctccccaaa acgggagccg ttgtcatgtc cccgccgatt
     5341 gacagtggta tcaattgcca gatctgtctg ctacgatgcg aggagggtcc cggacaaccg
     5401 gtctgtttgc ggcagcgtga ccgcgcgaaa gaaaagtatc ggacatgcgc agatccgtgg
     5461 tgtctctggc gttcgtcttg accatggtgc ttggggctgt gcctggtcct gcccgcgtgg
     5521 cggtagcggc gcctgctgag ccggccgttt gtgtggccga aacgatcacc ggggttatca
     5581 gcactgccgg tgggcgattc ttggcaacag caatcgtcgt ggtagatgct taggttgggg
     5641 gagcaggcga gtttatcgcc cccagggcgg tgaatgactt ctgcccacca cctgcttaga
     5701 aatgtctgtc atctcgatgg agtagccgtt catatcctcg ttcaacatga aggacgcccg
     5761 gcattatgcc gggcgtcctt ccttgcgcta gctggcacct tccggtgccc gtcatccttt
     5821 cagggccggt caagctcgcg gggatggcac attgggtgcg acggcgtgtc tccatggata
     5881 tcgctcagtg agaggcaggg ggatgacaga tcagcatggc gcgagcttga atggcaaggt
     5941 ggcaatcgtg acgggtgcga gcagcgggct gggccgggcg acggcgatgg ccctggctca
     6001 gcatggcgcc catgtcgcgt tgattgcccg cagcggtgct ggccttcagg atattgctca
     6061 agagattgcc tccggcagcg ggagccgcgc cctgccgctg cccctcgacc tcgccgatga
     6121 gacggcgctg gtcggggcag tggctcgcac cgtggcggat ctgggccggg tcgatatcct
     6181 gatcaatgcc gctggcacgg acaagccggg tcccgccgag accctgacga cggaggactg
     6241 ggaccaggtc ctggctgtca acctgcgtgc acccttcatc ctcgccaggg aagtcttccc
     6301 gcatatgcgg caggcgggtg ggggaacgat cgtgaacgtg tcgtcggtgg ccggcaagcg
     6361 tgggtgggcc aacgcgagcg cctactgtgc ctcgaagttc gggttgaccg gcctgacgca
     6421 atccctggct gctgaaggca aactgcacgg catccgggcg tcgatcctct atccgggcgg
     6481 catggccacc ggctggggga aatggtccgc ggcggagcgt gatgatgcat tggaggaacc
     6541 gccgccaccc acaaaagccc tgccgccgga cgaggtggcg gccctgatcg tatggatggt
     6601 cacggcaccc gctgaactcg tgctcaacga ggcggtcgtc agcccgctcg aagaagaggg
     6661 ctggccgtaa tgccatgatc ccgatgggcc gattgcctgg tagtcggatc gtaatgatgg
     6721 cattgagatg ggccgctccc gctcaatgcc tgcggaattg cctgattggc cgcaggccag
     6781 cgaaccctac acgaagcgtg gtgaccggga ccgagtttgg cctcgtgaac ccggcaacgg
     6841 agcttgcatg acccacgcgg gtgcctcgtt cgcgagcacc gcttcgcgtg ggattcttcg
     6901 ctttgctcag aatgacgggg tatgaggcct ccacggcact cacgaatgag atcgccccta
     6961 ccagacatcc ctcgcgcccc gtcaggagga cccgttcgac acgctgaggg taggctattg
     7021 ccctcaaatc ccgcttccct cgccgcaagt ccaagggtga gccctccggc gacaggcggc
     7081 agcgacccct tcgctacaga cagacgacct ctctgaaaac agctttagcc ggccctccta
     7141 atcggcgatg tctaccgccg ggacgctcac cgccatgaac gggcgcctgt cgacatctcc
     7201 cgggagctcc ggtatcgccg ctcgggcgtt gactccgccg tcgatcggcg tagagtggga
     7261 gtgaggcaac atcacggttt tgagctggag agccagcaaa aggaggaaca ccatggccca
     7321 ggaatcagcg aaggcggcca ccgaactcgt cgccgaggcg aagagccgga cggagaacct
     7381 aacgccggac gacgtcaccc gggagatggc ggaggcggat gccgtcctca tcgacctgcg
     7441 tgaacctcag gagcgagccc agaacggcac catccccggt gcaatccatg caccacgcgg
     7501 gatgctcgag ttctacgccg atccggccaa ttcgtaccat cggcccgaat tcgaccccaa
     7561 ccgtcggaca atcctccact gtgcctcggg cgggcgttct gccttggccg cggacacgct
     7621 gaagcaattg ggctacagca acgtcgcgca cctcgacggc gggatcaacg cctggaagga
     7681 aaccgggcac gaggtggaag agggcggctc ctcctaggtc agctgatggc gctagcgcag
     7741 catgctcggc gcgttccaga gaaggacctc tccttgtggg aggtccttct ccgtgcccgg
     7801 agagtcggtt cctagcggca attgccaatg ctcgttgtgg gccactatgg gcgggcccgg
     7861 tctacgttca gcgccagtaa ccgcgacagg atggcgtcct ccgcgttcgc aaacgggcgc
     7921 ataatgcagg tacgatgccc tggacggggc gattgcaggc tacctctccg gaatctcgat
     7981 cggtgggccg atcacggtaa ttccgtaacg cttcccgatc tcatccagtg ccgcttcgtc
     8041 aggtggcccc tgcagggatg caaaatacgc ggcgaactcg gcccacatgc cctccaggcc
     8101 agccggcacc gcgacaccca ccacgcggac cggatcgttg gtgaggttgg cgaatgtgtg
     8161 tggtacccct cgcggcagcc acgcgatccc gccagccggc acatcgaacc gctcgctgcc
     8221 tagcacgacc gtcaagactc cctcgacgac gtaacttgcc tcatcctcat gtgtgtggat
     8281 gtgaagagat ggtcccgcgg acatgggtgg catggttgcg tcgccgatga tcagtagccc
     8341 gccggtctca gccccgcgga cccgggttcg ccaaggccct ccgccgatgc cacgggattc
     8401 gtccggcccg aggacaaacg gctcggtacc tggtgtcaca cggtcttcca taaccatcct
     8461 ccgcatcgtg acaggcacga ccgtgagcgg tgctccggct gttagtcggg acgtcgccct
     8521 tgttcgtgga tttcgttcgc cgaagggacg gggtcgggat cagcgagtat ccacccggca
     8581 tcccctttct ccgaaccgat cggagtccag gtcacctccc acacgatgcc gttgttgtcg
     8641 acgaacagga actggcgcat cggtccctca tgcaaccact ccgtgacctc gacatctgcg
     8701 gagacaagct gcgcccgaag agcctccagc cccgcctcct tctcgacctt cagtgccagg
     8761 tgctagaagg caccgggcag aaaagcgaat gcccggctcc aacccgctgg cacggcggac
     8821 atggcatggt ccggatgctc gaagaagccg atcaggaaac cgcccgcatc aaacatgtaa
     8881 tggcgtccat ggatagggtt ggctcccacg ctgcccacga gtggcatacc aagcagttgg
     8941 tgatagaagc gcacggtcgc atccatatcc ctcgtcgtca gggcgatgtg atgagcaccg
     9001 tgccagcgtg gcccgccggg aaccatatcg ttcatgcgcg actccttcaa cggtgttttg
     9061 acttc
//
LOCUS       DASYWM010000336         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_450356, whole genome shotgun sequence.
ACCESSION   DASYWM010000336 DASYWM010000000
VERSION     DASYWM010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450356"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1447)
                     /locus_tag="VGR29_13205"
     CDS             complement(248..1447)
                     /locus_tag="VGR29_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV2074581.1"
                     /translation="MVTVTKLTQDVDEILPGVIADRRHLHENPELGFQEVKTAAFVKQ
                     RLEALGVEDIRTGINKTGVTGLVRGTAKGPGRNVLVRADMDALPIHEENEVDYKSQND
                     GVMHACGHDAHTAILLGLARVLTDRKSEFSGTVKLLFQPAEELPPGGAKGMIEEGVLN
                     DPPIEAVFGLHMSQGDPVGKIMVGAGPVMAAADGFKIIIQGKGGHAAYPSESIDPVVI
                     GSQIVVALQSLVSRETDPMQSAVLSTCVFKAGDAFNVIPDTAELAGTVRTFKTEVQDH
                     IERRIGEIATGIAESMQATAEVTYTRGYPATVNDEAMTQLARESAIQVVGEENVIEME
                     PKMGAEDFSYFLQERPGSYFFVGSKNEERGLTWGHHHPKFDIDEESLGAGLGTMATSV
                     LTYLTGP"
     gene            1639..1926
                     /locus_tag="VGR29_13210"
     CDS             1639..1926
                     /locus_tag="VGR29_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate o-methyltransferase 1"
                     /protein_id="HEV2074582.1"
                     /translation="MTEEQEPATQPLDENEATRLANAAVDSIGGPRSVYRNPRMAYAF
                     NATRMVEIEGHQVEIRYGEISTPAIVTIEGWVYQIYDEEIELLMRPPKKKS"
     gene            complement(2015..2353)
                     /locus_tag="VGR29_13215"
     CDS             complement(2015..2353)
                     /locus_tag="VGR29_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074583.1"
                     /translation="MGKDDLRQELWIPDNPDGSGWAGIDWVSAVRGSRYHYRHGAPHD
                     DAAGALPSFYKVVTRSETEQIPTPHENTYYVPALRLSDFLSELIWSSGSETIWHIEPC
                     EDPPPEARVK"
     gene            complement(2353..2928)
                     /locus_tag="VGR29_13220"
     CDS             complement(2353..2928)
                     /locus_tag="VGR29_13220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV2074584.1"
                     /translation="MMKDRPTQRSGGGIVSRSGQRSGPRDVDADNLPELMRGWRRRWA
                     SGVAVATARAADGSLRGITLTAVMPVSLDPPYLAFALALEGEFLGIVRGADACCVHIV
                     DRDQEFLSERFAGRAPLPDGIFTGVPYEMRGGLPVLAGVSAWAMGSVERIDATGDHAL
                     VILQVTAAGIGQDTDDPLLSYEGRYRGIEAS"
     gene            complement(2933..3415)
                     /locus_tag="VGR29_13225"
     CDS             complement(2933..3415)
                     /locus_tag="VGR29_13225"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2074585.1"
                     /translation="MRNSNRLPASSVPRYEEPRPVRYTLSDLEQETGVNARTIRYYIT
                     EGLLQPAYGRGPTATYDPDHLLRLRMIQQLKDERLSLKDIKERLSHLTPEDIAVILKI
                     QMQPETESWRRYVLHPEFEISVKETSPGDRAMTHAHAFDLIIEYARTVLEDLDRPEKE
                     "
BASE COUNT          682 a         1118 c         1006 g          697 t
ORIGIN      
        1 cagcaacgaa cttcgcggtt tcgtattcct cgaatgccag ttccgggttc tcgtggagat
       61 gccgccggtc ggcgacaacg ccgggaagaa tctcgtcgat atcattgcgc agtgttgcgg
      121 ttaccatgat cgcgattgcc ctttctgata gaggcgtgct ggtcaagatg ggatcgaaca
      181 tccgggaatg tgtcttagta cgggaccccg cctgagcgaa atccgacgtg ccctgccagg
      241 agcgagacta tggacccgtc aggtacgtga gcacgctcgt cgccatcgtg ccaagtccgg
      301 cgccgagcga ctcctcatcg atatcgaatt tcggatggtg gtgcccccac gtaagccctc
      361 gctcctcgtt cttgctgcca acgaagaagt acgagcccgg tcgctcctga aggaagtacg
      421 agaagtcctc tgcacccatc ttgggctcca tctcgatgac attctcttca ccgacaacct
      481 ggatcgccga ctcccgcgcc aactgggtca tcgcctcatc gttgaccgtg gcgggatagc
      541 cccgggtgta ggtcacttcg gcggtcgcct gcatcgactc tgcgataccg gtcgcaatct
      601 cgccaattcg ccgctcgatg tggtcctgga cttccgtctt gaaggtgcgg acggttcccg
      661 caagttcggc ggtatccgga atcacattga acgcatcacc ggccttgaag acgcatgtgc
      721 tcagcactgc gctctgcatc gggtcggtct cgcgcgaaac cagtgattga agcgcgacaa
      781 cgatctggga accaatgacg accgggtcga tactttcgct tggataggcg gcgtgaccgc
      841 ccttgccctg gatgatgatc ttgaagccgt cggccgcagc catcaccgga ccagcgccga
      901 ccatgatctt gccaacagga tctccctggg acatatgcaa tccaaagacg gcctcgatcg
      961 gcgggtcgtt cagcacgccc tcctcgatca tgcccttcgc cccacccggg ggaagctctt
     1021 cagcgggttg gaagagcagc ttcacggtgc cggagaactc gctctttcgg tctgtcaaca
     1081 cacgcgccaa cccgagcaga attgccgtgt gtgcgtcatg tccacaggcg tgcataacgc
     1141 catcgttctg cgacttgtag tcgacctcgt tctcctcgtg aatcggcaac gcgtccatat
     1201 cagcgcggac cagtacgttt cggcccggtc ccttggcagt gccgcggacg agacctgtca
     1261 ccccggtctt gttgatcccc gtgcggatgt cctcgacacc caacgcctcc agccgctgct
     1321 tcacgaatgc cgcggttttc acctcctgga atcccagctc cgggttctca tgaagatggc
     1381 ggcgatcggc gataacccct gggaggatct cgtcgacatc ctgggtcagt ttggtaacag
     1441 tgaccatgaa cctcgtactc cttgtgaatg tggatgcttt tgcccgtacc ggaaagtgaa
     1501 cccgcacggc acacggggca ccataccaga cctggcgctc cccgcaaacg catcgcggct
     1561 gacatggggt gatagcatca gggacaacct cggccgccag gcacggtctg agatcgacac
     1621 gctgaatggg agccagaagt gacagaggag caggaaccag caacacagcc gctggacgag
     1681 aacgaggcca cccggctcgc gaacgccgcg gtcgactcga ttggtggccc gcgctccgtc
     1741 tatcgcaacc cgcgcatggc atacgccttc aatgccacgc gcatggttga gatcgagggg
     1801 caccaggttg aaattcgtta cggtgagatc tcaacaccgg ccatcgtgac catcgaggga
     1861 tgggtgtatc agatctacga tgaggagatc gagctcctta tgaggccgcc gaagaaaaag
     1921 agctagtctg cgggcatgct catgatcccc ggcgcgtcgc cgcctgatgt tcagcccgac
     1981 gtgtcatttt tccgcttgtc cgccgccctc gtcattactt cacccgagcc tccgggggcg
     2041 gatcctcgca tggctcgata tgccagatcg tttcagagcc gctgctccag atcaattccg
     2101 agaggaaatc ggagagcctc agtgctggaa cgtaatacgt gttctcgtga ggcgtgggga
     2161 tttgctctgt ctcggagcgg gtaaccacct tatagaagga cggaagggct ccagccgcat
     2221 catcatgggg tgcaccgtgc cggtagtgat accggctgcc acggacggcg ctgacccagt
     2281 cgatacccgc ccaaccgctg ccatcgggat tgtccgggat ccacaactcc tgacgcaggt
     2341 cgtccttgcc catcaggaag cctcgatccc acgatagcgc ccctcatagc tcaggagtgg
     2401 atcatcggta tcttgcccga tacccgcggc tgtcacttgg aggatgacca gcgcgtgatc
     2461 accagtcgcg tcgattcgct ctaccgagcc catggcccac gccgatactc cggcgagtac
     2521 tggtagcccg ccgcgcatct cataaggaac cccggtaaag atcccatccg gtagcggagc
     2581 tcgccccgcg aagcgctcgc tgagaaattc ctggtcacga tcaacaatgt ggacgcaaca
     2641 cgcgtctgcc ccccgcacaa tcccaagaaa ctccccctcc aaggccagtg caaacgcgag
     2701 atatggagga tcgagggata ccggcatcac ggcagtcagg gtgatgcccc ggagcgagcc
     2761 atctgctgcc cgggctgtag caacagccac tccactcgcc caccgtcgcc tccagccgcg
     2821 catcaactcc ggcaggttgt ccgcgtcgac gtcgcgtgga ccggatcgct ggccactgcg
     2881 ggagacgatg ccaccaccgc tacgctgggt gggccggtcc ttcatcatgg cgtcactcct
     2941 tttccgggcg atcgagatcc tcgagcacag tcctcgcata ctcgataatc aggtcgaagg
     3001 cgtgagcatg ggtcatggcg cggtcacccg gtgatgtttc cttcaccgat atctcgaatt
     3061 caggatgcag cacgtaccgc cgccagctct ccgtctccgg ttgcatctgg atcttcagga
     3121 tgaccgcgat gtcctccggt gtaaggtgcg agagtcgctc cttgatatct ttcaacgaga
     3181 gccgctcatc cttcaactgc tggatcattc gcaggcgaag caggtggtcc ggatcgtagg
     3241 tcgccgtcgg cccgcgcccg tatgccggct gcagcagccc ctcggtgatg tagtaacgaa
     3301 tggtgcgggc gttcaccccc gtttcctgct caaggtctga gagggtgtac cgaacagggc
     3361 ggggctcctc ataacggggc acgctgctcg ctggcaatcg attggaattt cgcacggtcc
     3421 acctccctgc tcggcaagca cgggcagaag tcaaacgaca cttccctgtc attatgaccg
     3481 gccctgattc ggagcgcatc cta
//
LOCUS       DASYWM010000337        11794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_243623, whole genome shotgun sequence.
ACCESSION   DASYWM010000337 DASYWM010000000
VERSION     DASYWM010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 11794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11794
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243623"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VGR29_13230"
     CDS             <1..544
                     /locus_tag="VGR29_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV2074586.1"
                     /translation="VLANEPILIIGAGGLGLMALTVLQGMGGHGAIVADIDASKREAA
                     LKNGAYAAIDPTVPNAVDQIKAASPHGRGLRAAIDLVGNPSTAQLAIDSLVKGGKLIA
                     VGLMGGEITLPLPLIPMRALAIEGNYVGSLEDMRELMALVREKQVPAPPLTKRPLDQA
                     QAVIDELRGGRITGRAVLIP"
     gene            complement(516..1139)
                     /locus_tag="VGR29_13235"
     CDS             complement(516..1139)
                     /locus_tag="VGR29_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV2074587.1"
                     /translation="MSRILGIVLAAGMSRRLGRPKQLLMLDGTPLIAHVVDRALASTL
                     DEVILVTGAHGADVRAATEDRNVLLVHNPDFEEGQGSSLAAGITALDADVDAAVVLLG
                     DQPGISPEVINRAIKARRESGAPVVMARYGNQRGHPVLFGRELFSKLGNLTADTGGRE
                     IVRDHQDSLVLVDGGADAPPPDVDTEEAWAQLQSQWLSLRGSAPPAR"
     gene            complement(1136..1900)
                     /locus_tag="VGR29_13240"
     CDS             complement(1136..1900)
                     /locus_tag="VGR29_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEV2074588.1"
                     /translation="MSRPGFERVRELVDQGVPVAVATIIRGEGLGSKMIIMQDTVEGT
                     LGPRDLDDQVASDGRELLAAERSESRTYGDREIFLDVYPLPPHLVIFGAVHVAQALSN
                     FAQALGFQVTVVDARRALATRKRFPNVEKLITLWPDDAYQQLAVGPNTWIAILTHDPK
                     FDEPALLGALKTNARYIGAIGSRKTNLDRRERLSTAGVSDEDLARVRGPIGLDIGGQT
                     PEEMAIAILAEMIAVRYGRSGGYLVHSKGNIRGDAG"
     gene            complement(1897..2217)
                     /locus_tag="VGR29_13245"
     CDS             complement(1897..2217)
                     /locus_tag="VGR29_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEV2074589.1"
                     /translation="MSEWLPEIDEWKRDGKQVALATVTKIEGSAPRPVGARLAVTQDG
                     EMAGSVSGGCVENAVVLEAQQALKSGTPRLLRYGISDEMAWDVGLACGGTIEVFVEPM
                     EAPE"
     gene            complement(2243..3490)
                     /locus_tag="VGR29_13250"
     CDS             complement(2243..3490)
                     /locus_tag="VGR29_13250"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2074590.1"
                     /translation="MGQNLIHFGRRLREAGLPVGSGQIISLFEALQAVTVGQRDDVYH
                     AAKAVLITRPEQIPAFDIEFSRFWKDLLSRAPMLLEEYLQDDEESIGAPRKPSEASDD
                     PQSKQGEAGSSSDKTMLAIEGTDDATDDSNAEDFEAPPEDVLIFSARDAFRKKDFSQY
                     TPEEIAEARRIMSKMHWKLGTRQTRRKERAIRGEFIDHRAMLRSALRQGGVPLELKWR
                     KRKEKMRPLVLICDISGSMDRYSRLLLQFVHTLEAGLDSVEVFVFGTRLTRITRELRK
                     RDVDQAITDVVNAVDDWSGGTRIGEAIRDFNFTWSRRVLRSNATVVLISDGWDRGDPY
                     LLGTEMARLQRSCRRLIWLNPLLGAPGYQPLTQGIRAALPYVDYFLPVHNLRSLEALA
                     QLLSALDDQPPLRRQQGAEAFRH"
     gene            complement(3552..4493)
                     /locus_tag="VGR29_13255"
     CDS             complement(3552..4493)
                     /locus_tag="VGR29_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV2074591.1"
                     /translation="MNAATTPLTTQAERPTFDSVDQLEQILSAQRYVTDRGLATTVYL
                     ALKLGKPLLLEGEAGVGKTEIAKVLSAALQTPLIRLQCYEGLDANSAIYEWDYPRQML
                     FLRSLEATTEVTTETIRRDLFSDDFLLKRPLLQAIDAAHDRSPVLLIDEVDRADQELE
                     AFLLELLSDFQVTVPELGTIRALHKPIVILTSNRTREIHDALKRRCLYFWVDFPTYQK
                     ELRILSAKVPDAPQVLAEQICAFTQGLRQIDLFKSPGMAETIDWAEALLALGTNQLQD
                     EVVQDTLGVILKYQEDVDKVSGDVAQRLVARATHASR"
     gene            5077..5736
                     /locus_tag="VGR29_13260"
     CDS             5077..5736
                     /locus_tag="VGR29_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2074592.1"
                     /translation="MSYDPNERVTTPDVPFTGTADMARSHADSVEVKNRVQFGPIVAG
                     ILTAIASMLVLTVLGLAIGASALEPREVGEGVGTAATIWGIASAIISFFLGGWVAAKT
                     AAVAGVGSGMINGFIVGAGILALVLWLVGSGLGAAIGLIGSNVGDIANIVQDAGVTTD
                     EAQQQTEQATTQVEAPNSFEAVRDSAWGTLAGLLLPLVAGAAGGAVGHNKRSDVIQSG
                     R"
     gene            complement(5816..6535)
                     /gene="tsaB"
                     /locus_tag="VGR29_13265"
     CDS             complement(5816..6535)
                     /gene="tsaB"
                     /locus_tag="VGR29_13265"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV2074593.1"
                     /translation="MTHLESGTLPSIPASGDWVLAIDTSTEQAGLALGHGSCLFERSW
                     EAGRTQTTSVLPAIDALLREARIKVSDLAAIAIATGPGTFTGLRVGMSIAKGLVLARD
                     LPLIGIPTLDIAASAVNDVTELIAVLPAGRGRVVWLRYGPYADQEPRNTTVPQLVEYL
                     TDIPEVLVIGELAVEHQQTIERSHTHTRWENRQPGILLERARERWLRGEADDPVTLEP
                     TYLHGVTVIAGPVRDRLRKED"
     gene            6745..7725
                     /locus_tag="VGR29_13270"
     CDS             6745..7725
                     /locus_tag="VGR29_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2074594.1"
                     /translation="MAIHSGTAPQPLQERAEDPAKAYHTPKRGVKWFAGKEWLLFLLF
                     IFPNFFFLFMFTYWPLWENVKLSLQQTDLLAFSGTPKYVGMENYEWLFGNRTFRYVVQ
                     NTVIFIVSCVGLTLIFGLSIALLLNERLKLRNGVRALVFAPYLLSGAAIGIVWAYIFD
                     PRFGLLAQILDWFSIPSPHWLDRPAWALPAIIIVYVWKNLGFAVVIFLAGLQGIPKDL
                     YDAAKVDGAGPWWRFKSVTLPMLSPISFFLMVTSVLATFQAFDIQQVMTAGGPAYATT
                     TLVYYVYEQSFGQGANFGRAAAAAIILFLSMLVITAIQMFYTEKKVHYDG"
     gene            7718..8590
                     /locus_tag="VGR29_13275"
     CDS             7718..8590
                     /locus_tag="VGR29_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2074595.1"
                     /translation="MDRLNAHQKATVGHAFGYVGLLLVTLVIGLPLFWMLSGSLKNLQ
                     EIYTFPPEWIPRNPKWSNFREAWESMPFARFYVNSLIITFFGASAQLFCGVLCAYAFA
                     FLHFPAKNWLFLGVLAALMVPPEVVILPNFLFFGNTVRDWFGLEDNWVNTYQAIVLPG
                     AATAFGTFLMRQGFMALPKDVLDAAKVDGAGHLRTMFGIVLPMAKPIVITFAMISMVN
                     KWNDYLWPLVITRTTEMRPITVGVRLLFDAEGNNNWGVIMAGTSFVILPLLVVYLLGQ
                     RYIIEGLTAGAMKG"
     gene            8778..10094
                     /locus_tag="VGR29_13280"
     CDS             8778..10094
                     /locus_tag="VGR29_13280"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2074596.1"
                     /translation="MIKDFSRRSIIKGAASAAVGASVLGAPSKTFAAPARLQSTGSNA
                     EITLWTAFGSGVNGEAQAKLVEDFNAQGNGVTVTAQVYESYEAVAQALITGLQTGDVP
                     DVATLSDVWWFRFYLSQALADLTEMAQEPDDYVETLYADYQRNGGQFGVPFARSTPLL
                     YFNRDVLEAAGLDESIFETWSSTVEAAPDLLAAGDNMTAAFGFGNAASYGAWFLHGPV
                     WAFDGRYSDEEFNILIDQEGAVNCGEFMREFVQSGNAAAVTDPSTDFQTGGIAATIQS
                     TGALGGITEAAQFDVGTHFLPEEIEFGCPTGGTGLSVMAGASDENKQAAMDFISFSTN
                     TENATYWAMTTGYMPVRNSAIESEEFQTFITENPNNRVAIEQLPLTRPQDTARVFIPN
                     GDQILGQGWEQVLVNNVPAAEAFAEVAQILNEEKEPVLEALQELEG"
     gene            complement(10214..10657)
                     /gene="tsaE"
                     /locus_tag="VGR29_13285"
     CDS             complement(10214..10657)
                     /gene="tsaE"
                     /locus_tag="VGR29_13285"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV2074597.1"
                     /translation="MNDELEIGSHAAMQAFGRAFAQQLGAGDVVLLHGDLGVGKTTLT
                     QGIAAGLGVEGPVQSPTFTLVQEHLGRVLNLYHLDLYRLDDPSELESMGYETYLEPED
                     GVSVVEWPERAGDWVPPVFWLIQIAHRGGDHRRLTCRFVGEVTDS"
     gene            complement(10666..11277)
                     /locus_tag="VGR29_13290"
     CDS             complement(10666..11277)
                     /locus_tag="VGR29_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV2074598.1"
                     /translation="MQVESAGAGRDTDSAAVLRAIRERRSIGKCTEEIPDPEIIRQVL
                     EAGTWAPNHHLTEPWRFVVLSGDARTGLGEAMGEAAAREAANVEAGETARSRAVGKPL
                     RAPYVIAIYADPDPSAPEIEEIASAAAAAQNMLLAAHALGLAAMWRSGALIYSPEVRR
                     YLNLPERAVMLGVLYVGYPAMEPPQRERRPMDDVVAWWDAAPE"
     gene            complement(11435..>11794)
                     /locus_tag="VGR29_13295"
     CDS             complement(11435..>11794)
                     /locus_tag="VGR29_13295"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEV2074599.1"
                     /translation="TAAMDLSDGLLGDLPKLLAASGVGARLDSRLVPVIPAVRALFPI
                     HWLELALRGGEDYELLATVRPDALEEIQREAARVGATLTEIGEIVSSPAGESMLIITD
                     AEGVTKAVPEGAFDHFG"
BASE COUNT         2288 a         3683 c         3369 g         2454 t
ORIGIN      
        1 ggtcctggca aatgagccga tcctgatcat cggcgctggc gggctggggc tcatggcgct
       61 gaccgtactc cagggtatgg gcggacacgg cgcgatcgtt gccgatatcg atgcttccaa
      121 acgcgaggcc gccctgaaaa acggcgccta cgccgcgatt gatccgacgg ttcccaatgc
      181 ggtggaccag atcaaggcgg cgtctccgca tggccggggg ctccgggctg cgatcgatct
      241 ggtcggcaat ccctccaccg ctcaattggc aatcgattca ctggtgaagg gaggaaaact
      301 gatcgcagtg ggactcatgg gcggagagat aacccttccg ctcccgttga tcccgatgcg
      361 agccctcgca atcgagggga attacgttgg cagcctcgaa gacatgcgag aactcatggc
      421 attggtgaga gagaaacagg tccccgcgcc gcctctcacc aagcgtccac tagaccaggc
      481 acaagcggtg attgatgaac ttcgcggcgg caggattacc gggcgggcgg tgctgatccc
      541 ctgagactca gccactggga ttgcaattgg gcccaggctt cttcggtatc gacatccggc
      601 ggcggtgcat cggctccgcc gtccacgagc accagggaat cctggtggtc acggacgatc
      661 tcgcgaccac ccgtgtcggc ggtgaggttc cccaacttcg aaaagagttc gcggccaaag
      721 agcaccgggt gaccccgctg gttcccgtac cgggccatca ccactggagc gcccgactcc
      781 cggcgtgcct tgattgcgcg attaatgacc tctggggaaa ttccgggctg atccccgagc
      841 aacaccactg ccgcgtccac atcggcatca agcgcggtga tccctgccgc aagcgatgat
      901 ccctgtcctt cctcaaaatc aggattgtgg acaagaagga cattcctgtc ctcggttgct
      961 gctcgcacgt cggcaccatg agcaccggtg acgaggatca cctcgtccag ggtagaggcc
     1021 agcgcgcgat cgacgacgtg cgcgatgaga ggtgtcccgt ccagcatgag gagctgcttt
     1081 ggtcgtccga gacggcgcga catgcccgcc gccagcacga ttccgaggat ccggctcacc
     1141 cggcatcgcc gcgaatgttg cccttcgaat ggacaagata gccaccgctc cgcccatagc
     1201 gtacggcgat catctcggcc aggatggcga tcgccatctc ttccggagtt tgacctccga
     1261 tatccagacc aattggaccc cggacacgtg caagatcctc gtcgctcaca ccggcagtac
     1321 tgaggcgctc tcgccgatcc agattggttt tgcggctccc tattgcgccg atgtaacgag
     1381 cattggtctt gagcgcgcca agcaatgccg gttcgtcgaa cttgggatca tgcgtcagga
     1441 ttgcgatcca cgtattcgga ccaacagcta actgctggta ggcatcgtcg ggccacaaag
     1501 tgatgagctt ctcaacattg ggaaagcgct tccgggttgc cagtgcccgg cgcgcgtcca
     1561 caactgtcac ctggaaaccc agcgcctggg cgaagttcga gagcgcctgc gccacgtgca
     1621 cggcgccgaa aatcaccagg tgcggtggca acgggtagac gtcgagaaaa atctcccggt
     1681 caccataggt gcgtgattcg gaccgctcgg ccgcaagcag ttcgcggccg tcggaagcga
     1741 cctgatcatc gagatcgcga gggccaagcg ttccctcgac ggtgtcctgc atgatgatca
     1801 tctttgaccc caggccttcg ccccggatga tcgtcgcgac tgcaacaggg acaccctggt
     1861 cgaccagttc gcgcacccgc tcgaacccgg gacgactcat tcaggggcct ccataggctc
     1921 gacaaagacc tcgatcgtgc cgccacatgc cagaccaacg tcccacgcca tctcatctga
     1981 gattccatag cgaagcagcc tcggtgtgcc ggatttgagc gcctgttgtg cctccagcac
     2041 gacggcgttc tcgacacatc cgccgcttac tgagccggcc atctcaccgt cttgagtcac
     2101 agcaagcctt gcaccgacgg gtctcggcgc agagccctca atctttgtga cagtagcgag
     2161 ggcaacctgt ttgccatccc tcttccactc gtcgatctct ggtaaccatt cgctcacggg
     2221 tatgctcctt tcccggcacg tctcaatgcc ggaatgcctc ggccccttgc tggcgtcgca
     2281 acggtggctg atcgtccagc gcgctcagca gctgagccag ggcctccagg ctcctgagat
     2341 tatgcaccgg caggaagtag tcaacgtacg gcaacgccgc ccggatgcct tgggtaagcg
     2401 gttgatatcc cggcgcgccc aggagggggt tgagccagat aagccggcga cacgaccgct
     2461 ggagcctggc catctcagtt ccgagaaggt agggatcgcc gcgatcccag ccgtcgctga
     2521 tgagcacgac tgttgcattg gatcgcagca ctcggcgact ccacgtgaaa ttgaaatcac
     2581 gtattgcctc accgatccgc gtgccgcccg accagtcgtc gacggcattg acgacatcgg
     2641 tgattgcctg gtcaacgtcc cgcttgcgga gttcgcgggt aatccgtgtc aggcgcgtgc
     2701 caaagacaaa aacctcaacg gaatcgagcc cggcttcaag cgtgtgcacg aattgcagca
     2761 gcagccgaga gtagcggtcc atggatcccg agatatcgca aatgagcacc agtggacgca
     2821 tcttctcctt gcgcttgcgc catttcagct ccagcggcac gccaccctgc cgtaacgccg
     2881 accgcagcat ggcacggtga tcgatgaact ccccgcggat tgcccgctcc ttgcgccggg
     2941 tctggcgtgt accgagcttc cagtgcatct tcgacatgat tcgcctggct tcggcaatct
     3001 cttccggagt gtattgcgag aagtccttct tccggaaggc gtcccgggcg ctgaagatca
     3061 gcacatcctc cggtggtgcc tcgaagtcct ccgcgttgct gtcatcggtg gcatcgtcgg
     3121 tgccttcaat cgcaagcatc gttttatcgg acgagctacc cgcttcgccc tgtttactct
     3181 ggggatcgtc gctggcttcg gaaggtttcc tgggcgctcc gatggattcc tcgtcatcct
     3241 gcagatactc ctccaggagc atgggtgcac gggacaggag gtccttccag aagcgcgaga
     3301 actcgatgtc gaatgcgggt atctgctccg ggcgggtaat cagcaccgcc ttcgccgcgt
     3361 ggtagacgtc gtcgcgctga ccaaccgtta ccgcctgcag ggcctcgaaa aggctgatga
     3421 tctgccccga cccaaccgga agccctgcct cgcggagacg ccggccgaaa tggatcagat
     3481 tctggcccac tctcgacgcc cgtacggcag actcatccat agtcaaaccg ttagtcgcct
     3541 cccggccgtt gtcagcgtga agcatgggtt gcccgtgcaa cgagacgctg ggcgacatca
     3601 ccactgacct tgtccacatc ctcctggtac ttcaggatga cgcccaacgt gtcctgcacc
     3661 acctcatcct gcaactggtt tgtcccaagt gcgagaagcg cctctgccca gtcgatggtc
     3721 tcggccattc ccggggattt gaacaggtca atctgacgca gcccctgcgt aaaggcgcag
     3781 atttgctcag ccaacacttg gggtgcatct ggcaccttgg ccgacaggat cctcagctct
     3841 ttctggtagg tggggaaatc aacccagaaa tacaggcagc gccgcttgag cgcgtcatga
     3901 atctcccgcg tgcggttgga cgtgaggatc acgattggct tgtggagggc acggattgtc
     3961 ccgagctccg gcaccgttac ctggaagtcg gagagcaact cgaggagaaa tgcctcaagt
     4021 tcctggtccg cgcgatcaac ctcgtcgatg aggagcacgg gtgaccggtc gtgggcagca
     4081 tcgatagcct gcaacagggg ccgcttgagc agaaaatcgt cgctgaagag atcgcgccgg
     4141 atcgtctctg tggtcacctc cgtcgttgcc tcgagtgacc ggagaaagag catctggcgg
     4201 ggatagtccc attcatagat ggcagagttc gcgtccaggc cctcgtagca ctgcaggcgg
     4261 ataagtggcg tctgtagagc agcggaaagc accttggcga tctccgtctt gccaacgccc
     4321 gcctcaccct ccagaagcaa tggcttgccg agcttcagcg cgagatacac cgttgtcgcg
     4381 aggccgcggt cggtaacgta acgctgggcc gacaatatct gctcgagctg gtcgacggaa
     4441 tcaaaggtcg ggcgttctgc ctgtgtcgtc agtggggtgg ttgcggcgtt caaaatagtc
     4501 cctcgttcaa gcgttgtcat cctggtcgcg aaccgaagca tcccgcgtgg agcacgtgtc
     4561 atgacatcag gtactgggaa gcggttggtc ccccaggtga taaagagacc agcttggcca
     4621 tgtgctctgg cgtccgctgt tcaccggact aaccaattgt ctctttatgg aaccgcgaga
     4681 tcggtaccaa cagcgacggt ggattccgga gccgtgcatt aaccgcttcg gacagcatcc
     4741 aaagtatccc tgttgcgcta ttggctgacc acacggcaac aggcgttgcg cgcttccgaa
     4801 tccccgaagt cgcaagcgga aagccccaga aggttgacga ggacgatcgg cgggcctgcg
     4861 ccttgaggag cccgttctcg ttcgccattc atggcggctc tgactctagc gaacccactt
     4921 cggtccccag acacggtacc gataacgtta gccccgccag ctcccttccc ctcgtgcctc
     4981 cgtcctgaag aagcgtggca ccgtgcatgc gaagactgcc ttgtcgatga attgtcggct
     5041 ttttcacaat tttagtattc gaggagttct taatagatga gttatgaccc aaatgaaagg
     5101 gtgacgaccc ccgacgtacc gtttaccggc actgcggata tggctcgcag ccatgccgat
     5161 tcagttgagg ttaagaaccg ggtccagttc ggcccgatcg tggccggtat cctcaccgcc
     5221 atcgcctcca tgctggtcct gacggtgctc ggcctcgcaa tcggcgcctc tgccctggag
     5281 ccccgcgaag ttggcgaggg tgttggcact gctgccacga tctggggtat cgccagtgcc
     5341 attatctcgt tcttcctcgg tggctgggtc gccgcgaaga cggccgctgt tgccggcgtg
     5401 ggtagcggaa tgatcaatgg cttcatcgtg ggcgcgggaa ttcttgccct cgtcctgtgg
     5461 ctggttggct ccggtcttgg cgcggcgatc ggtcttattg gcagcaatgt cggtgatatt
     5521 gccaacatcg tgcaggacgc cggtgtcacg accgatgaag cccagcagca gactgaacag
     5581 gcgacgacac aggttgaggc cccgaactcc ttcgaggctg tccgcgacag cgcttggggt
     5641 acgttggccg gtctcctgct cccgctcgtg gctggtgccg ccggtggagc agtgggacat
     5701 aacaagcgca gtgatgtcat ccaatccggg aggtaaattt cgctctatcc ggctacaagc
     5761 catcagcata aggaaggagg ggcctctcgg ttcctccttc tttatgttca atacattagt
     5821 cctctttacg aagccgatca cgcaccgggc ccgcaattac tgtcaccccg tgcaaatacg
     5881 tgggttccag tgtcaccggg tcatctgcct cgcctcgtag ccaccgctca cgcgcgcgct
     5941 ccagcaatat ccccggctgg cgattctccc atcgtgtgtg cgtatgcgag cgttcgatgg
     6001 tttgttgatg ctcaacggca agctcgccga tgaccaaaac ctctggtatg tcggtcaggt
     6061 attcaacgag ctggggcacg gtagtgttac gcggttcctg atccgcataa ggtccatatc
     6121 gcagccacac gacccgccca cgtcctgccg gcaagacggc gatgagttcc gtcacatcgt
     6181 tgacggcact cgctgcgatg tccagcgtcg ggattccgat aagaggaaga tcccgcgcca
     6241 gcaccagccc tttggcgatg ctcatgccga cacgcaaccc agtgaaggtg ccaggcccag
     6301 tcgcaatggc aattgcagcg agatcactga ccttgatgcg cgcttcgcgc aagagtgcgt
     6361 cgatagctgg cagcacggag gtcgtctgtg ttcttccagc ctcccaggat cgctcgaaga
     6421 ggcaagaccc atgacccagg gcaagaccag cctgctcggt cgaggtatcg atcgcgagga
     6481 cccaatcgcc actcgcgggg attgatggta acgtgccgga ttcaagatgc gtcacaggtt
     6541 gtgcggacct cccacaacgg actagtatgt taatgattta aggccacgcg acaggtgatc
     6601 attcacgtaa acctcggcgt atgtgcgctt gggtttctga agagcgctgg cctactggaa
     6661 gcttacgtca gcgtccttca tcccggctct cgcaataacc acccgggttg atactgcctg
     6721 acggattgac atcggggaac gtgcatggcg attcatagcg gtaccgcgcc acaaccgctt
     6781 caggaacgag ccgaggaccc agccaaggca tatcacactc ccaaacgggg agtaaagtgg
     6841 tttgcgggca aggagtggct cctcttcctg ctctttatct tccccaattt tttcttcctg
     6901 ttcatgttca cctattggcc cctctgggag aacgtcaagc tcagtcttca gcagaccgac
     6961 ctgctggcgt tcagcggcac ccccaagtac gtcgggatgg agaattatga gtggttgttc
     7021 ggcaacagga ccttccgata cgttgtccag aacaccgtca tctttatcgt ctcctgcgtc
     7081 ggcctgacac tgattttcgg attgtcgatt gcgttgctcc tcaacgagag gctaaagcta
     7141 cgaaacggcg ttcgggcact ggtctttgcg ccctaccttc tctccggtgc agccatcggt
     7201 atcgtctggg cctacatctt cgacccgcgc tttggcctgc tggcgcagat tctcgattgg
     7261 ttctcaatcc cgtcgccgca ttggctggac cgccctgcct gggcgcttcc agcgatcatc
     7321 atcgtctatg tctggaaaaa cctgggcttc gctgtcgtga tcttcctcgc cggactccag
     7381 ggcatcccca aggatctcta cgatgctgca aaggtcgatg gcgccggccc ttggtggcgt
     7441 ttcaaatcgg tcaccctgcc aatgctctcg ccgatctcct tcttcctcat ggtgacgtcc
     7501 gtgctcgcga cgttccaggc ttttgacatc cagcaggtca tgaccgcagg cggccccgcc
     7561 tatgccacga ccactctcgt ctattacgtc tacgagcaga gcttcggtca gggcgccaat
     7621 ttcggtcgag ctgccgctgc tgccatcatc ctcttcctct cgatgttggt aatcaccgca
     7681 attcagatgt tctatacgga gaagaaggtt cactacgatg gatagactga acgcgcatca
     7741 gaaagcgact gttggccatg cgtttggcta tgttggtctt ctcctcgtga ccctggttat
     7801 cggtctgcca ctcttctgga tgctttccgg ctcgctcaag aatctccagg agatctacac
     7861 cttccctccg gaatggattc cgcgcaatcc caagtggagc aacttccggg aagcgtggga
     7921 gtcaatgcca ttcgcccgat tctacgtgaa ttcgctcatc atcacgttct tcggggcaag
     7981 cgctcagctc ttttgcggtg tactctgcgc ctacgccttc gcgttccttc actttcctgc
     8041 caagaattgg ctcttcctcg gtgtgctggc ggcgctcatg gttccgccgg aagtcgtgat
     8101 cctgccgaat ttcctcttct tcggaaacac cgtgcgtgac tggtttggcc ttgaggacaa
     8161 ctgggtcaac acctaccagg cgatcgtgct acctggcgca gcgacggcat tcggtacctt
     8221 cctcatgcga cagggcttca tggcgctgcc gaaggacgtg cttgacgccg ccaaggtgga
     8281 tggcgccgga cacctacgca ccatgttcgg catcgtcctg ccaatggcga agccgatcgt
     8341 tatcaccttt gccatgatct caatggtcaa caagtggaac gactacctct ggcccctggt
     8401 gatcactcgt accaccgaaa tgcgaccgat tacggttggc gttcgcctcc ttttcgatgc
     8461 tgaaggcaac aacaactggg gcgtaatcat ggccggaaca tcattcgtca tcctgcctct
     8521 actcgttgtc tatctcctcg gccagcgcta catcatcgag ggcctgaccg ctggagcgat
     8581 gaagggttaa ggatcgaaac cttaacaaag aggcgatgat gcggcataat caatgcgcct
     8641 acactgttta acacgcgctc atcagcggct tttcatctcc aataccgcgg gcgcctgcac
     8701 tcaggagtag acgggattcc atggtggaat tcccggatta gctcaatggc ttgagcgaac
     8761 aggaggatcg gataacgatg attaaggact tttcacgtcg ttccatcatc aagggcgctg
     8821 ccagtgcagc ggtcggtgct tcggtgctag gagcacccag caagactttc gccgctccag
     8881 cacgtctaca gtccaccggc tcgaatgccg aaatcacgct ctggacggcg tttggaagcg
     8941 gcgtcaatgg cgaggcccag gccaagttgg tggaggattt caacgcccaa ggtaatggcg
     9001 ttaccgtcac cgcacaagtc tatgagagtt acgaagctgt cgcccaagcg ctcatcacgg
     9061 gcctgcaaac cggcgatgtg cctgatgtcg ccacactttc tgacgtatgg tggttccgct
     9121 tctacctttc ccaagcgctc gcagatctga cggagatggc tcaagagccg gacgactacg
     9181 tggaaacact ctacgctgac taccagcgca atggtggtca gtttggcgtg ccgttcgccc
     9241 gctcaacccc gctgctctat ttcaaccgcg atgtgcttga ggctgcgggt cttgatgaat
     9301 ccatcttcga gacgtggtcc agcaccgtcg aggccgctcc ggacctgctc gccgcgggag
     9361 acaacatgac cgctgccttt ggtttcggca atgccgccag ctacggcgcg tggttccttc
     9421 acggtccggt ctgggcgttc gacggccgtt attccgatga ggagttcaat atcctcatcg
     9481 accaggaagg tgctgtgaac tgtggtgagt tcatgcgtga gttcgtacag agcggcaatg
     9541 ccgctgccgt cacggacccg agcacggact tccaaaccgg tggaattgcc gccaccattc
     9601 agtccaccgg tgcgctcggg ggcatcacag aagcggccca gttcgacgtc ggcacccact
     9661 tcctcccgga agagatcgag ttcggctgcc caacgggtgg cactggtctt tccgtaatgg
     9721 ccggggcatc cgacgagaac aagcaggctg ctatggactt catctcgttc tcgaccaaca
     9781 ccgagaacgc cacctactgg gcgatgacga cgggctacat gccggtccgc aactcggcca
     9841 tcgagagcga ggaattccaa accttcatta ccgagaatcc gaacaaccgg gtggcgattg
     9901 agcagctgcc gctgacgcgg ccccaggata ccgcgcgagt cttcatcccg aacggcgatc
     9961 agattctcgg ccaaggttgg gaacaggttc tcgtgaacaa cgtcccggcc gccgaggcct
    10021 tcgcagaggt cgcgcagatc ctcaatgagg agaaggagcc ggttctcgag gctctccagg
    10081 agcttgaggg ctagacccct tcttcaggtc aattgcaagc agcaatggag aagctccggg
    10141 atgctaaccc ggagcttctc catttgctcg ttgacgatcg tgatggttat atcggagcga
    10201 ccggttgatt cgcttaagaa tctgtcactt cacctacaaa ccggcacgtc aggcgacgat
    10261 gatcccctcc gcggtgcgcg atctggatga gccagaagac cggtggcacc caatcacccg
    10321 cgcgctccgg ccactcgacc acactcacgc catcctccgg ctcaaggtac gtttcatacc
    10381 ccatgctctc cagctcagat ggatcgtcca gccgatagag gtccaggtgg tagaggttga
    10441 gaaccctccc tagatgctcc tggacaagcg tgaaggtggg gctctgtaca ggaccttcga
    10501 ctccgagtcc tgcggcgatt ccctgggtca gcgtcgtttt gccgacgccg agatccccat
    10561 gcagcagcac aacgtcaccg gcacccagct gttgcgcgaa cgctctcccg aatgcctgca
    10621 tcgcggcgtg ggagcctatc tcgagttcgt cattcacctg atgcattact cgggtgccgc
    10681 gtcccaccag gccaccacat cgtccattgg tcgccgctcg cgctgcggcg gctccattgc
    10741 cggataaccc acataaagga cccccaacat aacggcacgt tccggcagat tgaggtatcg
    10801 ccgcacttcc ggagagtaga tgagcgcacc ggatcgccac atcgccgcaa gccccagtgc
    10861 atgcgccgcc agcagcatgt tttgcgcggc tgccgcggct gatgcgatct cctcgatctc
    10921 cggcgccgag ggatcgggat ccgcatagat ggcaatcaca tagggtgctc gcaacggctt
    10981 gccaaccgct cgtgaccggg ccgtctctcc tgcctctacg ttcgcagctt cccgggcagc
    11041 cgcctccccc atcgcctcac cgaggccagt ccgcgcgtca ccgctgagga ccacaaaccg
    11101 ccatggctcg gtcaggtgat ggtttggcgc ccacgttccc gcctcgagga cctgccggat
    11161 gatttccgga tccgggattt cttcagtgca ctttccgatg ctccggcgct ctcgaatggc
    11221 gcgcagcacc gccgccgaat cggtatcacg tccagctcct gctgattcaa cctgcatgcc
    11281 actccaccat ttgggtgcac agtgttgcgc ctgtgcaacg tcgttcagga cgatccatat
    11341 ccgacttagt aagtcggtat ttatgcacgc aatgataggc gcatgggcgt tgacggagca
    11401 agcagccctt ccggatactt cgaagagtca ctcatcagcc gaaatggtca aatgcaccct
    11461 cagggacagc cttcgtcaca ccttccgcat ccgtaatgat gagcatcgat tcaccagcag
    11521 gactcgatac gatttcgccg atctcggtca gcgtcgcacc taccctagct gcctcacgct
    11581 gaatctcttc cagggcatcg gggcggaccg tggccagcag ctcgtaatcc tccccgcccc
    11641 gcaatgcgag ttcgagccaa tggattggga agagtgcccg taccgcggga atgaccggta
    11701 ccagccggct gtccaggcgc gcgccgacac cactggcggc cagcagcttg ggcaagtccc
    11761 ccagcagccc gtcactcagg tccattgccg cggt
//
LOCUS       DASYWM010000338         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U6281
            k141_243934, whole genome shotgun sequence.
ACCESSION   DASYWM010000338 DASYWM010000000
VERSION     DASYWM010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955733
            Sequence Read Archive: SRR12284406
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,662
            CDSs (total)                      :: 2,624
            Genes (coding)                    :: 2,605
            CDSs (with protein)               :: 2,605
            Genes (RNA)                       :: 38
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 15 of 19
            Pseudo Genes (internal stop)      :: 3 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243934"
                     /isolate="SMAG_U6281"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="39.9022 N 96.2267 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VGR29_13300"
     CDS             complement(<1..303)
                     /locus_tag="VGR29_13300"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV2074600.1"
                     /translation="MPDGAVPKPRLRDLGITIGAMPTGPLNAITDVTGVRVGHTTVIH
                     GEGARRPGEGPARTGVTAIHPHEGSAFTSMVPAAIEVLNGAGEITGRSQVDEYGLLE"
     gene            complement(386..1723)
                     /locus_tag="VGR29_13305"
     CDS             complement(386..1723)
                     /locus_tag="VGR29_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV2074601.1"
                     /translation="MTTADLGNIRTNTASHEETIEKYRRYVNTSFVAGIEPVVVGKAE
                     GSTVHDADGTVYLDCFAGISVVNAGHRHPRVLDAVRAQLDQVVHAATYIYHVPVVADL
                     AEKLAQVTPGDLTKSFFGNSGAEGIETAVRLAKAYTGRHEVISLTHSFHGRTAGTLSI
                     TGNKARKTRGGPYLAGVAFAPAPYVYRNPFGTDDPEVVADRCAEMVEWAISYQTSGDV
                     AAFIAEPVMGEGGILVPPSSYFSRIKEILDRHGILFIADEVQSGFGRTGKMFAIEHFG
                     IVPDIMVMAKGIADGFPLSATIARDEIADCLRPGEHLSTFGGNPVSCAAALANIAVLE
                     DEQLPQESARKGAHAIGRLQELAKRHELIGDVRGLGLMIGAELVTDRESKGPASKQAV
                     EVRRLCREAGVLIGVGGQAGNVLRIQPPLVISDEQLDHAIDTIDKALAIVAGQ"
     gene            complement(1809..2840)
                     /locus_tag="VGR29_13310"
     CDS             complement(1809..2840)
                     /locus_tag="VGR29_13310"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV2074602.1"
                     /translation="MDPASDTGLQLHSGPLNAVTDVPGITVGHYTHDEVRRGVTAVLC
                     QEGAAAGVSVRGSNPGTVNTDALAATAIGSLVHGIGLTGGSLFGLAATAGMAEWLFEQ
                     GVGLRMDGAVLPVMSGAVIFDLRLSDPTIVPTADWGRQAAEAATDGEIARGNVGVGAG
                     ATAGKGPGCIKTKGGLGTASLVLPGGIVVGALVVVNSLGGLIHPLTGKLYATDGGFDT
                     PLLYHLKDEVPDEVASRANTTLGLIATNADLEKSQLIKIADLAHDGLARAIRPMHTMR
                     DGDTIFALSPYVNRVRLEDTADAKLTDLVGAAAADAMVLAVLDAARQTEGIDAWPSVT
                     DAQAAFTSL"
BASE COUNT          582 a          995 c          930 g          623 t
ORIGIN      
        1 ttcaaggagc ccatattcgt cgacctgtga gcgtcccgtg atctcgccag cgccattcag
       61 tacctcgatg gcggccggaa ccatggaggt aaacgcggat ccctcatgcg ggtggatggc
      121 tgttacgcca gtccgcgccg gaccctcgcc cgggcgacgt gctccttcac cgtggatcac
      181 ggtggtatga ccgacccgca cacctgtcac atccgtgatg gcgttgagcg ggccggtcgg
      241 catcgcaccg atcgtgatac cgagatcccg aagacggggt ttgggtacgg caccgtcagg
      301 cattctcgcc accattgacc atttcgccat cacaagagtg ggtcaagaat ttgtccttgc
      361 tgaaagggtc cacacgctct actcgttact gcccagcgac gatagcgagc gccttgtcga
      421 tggtgtcgat ggcgtgatcg agctgctcgt cgctgatgac gagcgggggc tggatgcgaa
      481 ggacattgcc ggcttgccca ccgacgccga tgagcacgcc cgcttcacgg caaagacgtc
      541 ggacctcgac ggcttgcttc gatgctggcc ccttgctctc gcggtcggtg acaagctcgg
      601 caccaatcat caggcccaaa ccacggacat caccgataag ctcatggcgt ttcgcaagct
      661 cttggagtct cccgatcgcg tgtgcccctt tgcgggcaga ctcctgcggc agttgttcat
      721 cctccagaac agcaatgttg gcgagcgccg ccgcgcagga tacgggattg ccgccaaagg
      781 ttgaaaggtg ctctcccggc cggaggcagt ctgcgatctc gtcccgggcg atggtcgcac
      841 tcagcgggaa gccatcggcg atcccctttg ccatcaccat gatgtctggc acgatgccga
      901 aatgctcgat cgcaaacatc ttcccggtgc gtccgaagcc actctggacc tcatccgcga
      961 taaagaggat gccatggcga tcgaggatct ccttgatacg ggagaagtag gaggaagggg
     1021 ggacgaggat gccgccttcg cccatgacgg gctcggcgat gaatgccgcc acgtcgccac
     1081 ttgtctggta gctaatggcc cactcgacca tctccgcgca ccggtccgcg acgacctctg
     1141 gatcgtccgt gccaaacggg ttgcggtaga catagggagc cggcgcaaag gcgacaccgg
     1201 cgaggtaggg gccgccgcgg gtcttgcgag ccttgtttcc ggtgatggag agggtaccgg
     1261 cggttcgccc atggaaagaa tgggtgaggc tgatcacctc atggcggccg gtgtaggcct
     1321 tggctagccg gaccgctgtc tcgatgcctt ccgcaccgga gttcccgaaa aatgacttgg
     1381 tcagatcgcc cggtgtaacc tgcgccaact tctccgccaa gtcagcgacg accggcacat
     1441 gatagatgta ggtcgcagca tgaactacct ggtccagctg cgcgcgaact gcgtccagca
     1501 cccggggatg gcgatggcca gcgttgacga ccgagatacc cgcgaagcag tcgagataca
     1561 ccgttccgtc agcatcgtga acggttgagc cttccgcttt cccaacgacc accggctcaa
     1621 tcccggcaac aaaactggtg ttcacatagc ggcggtattt ttcgatcgtt tcctcatggg
     1681 aggcagtatt cgtcctaata ttgccgagat cagcagtcgt catctctcac tcctgtcctt
     1741 cagggcccat gtccagcggc ctctttactc caacacgaac tggtttcaat ttcgcgccaa
     1801 tccccgccct agagggaggt gaatgcggcc tgtgcatcgg tgaccgacgg ccaggcgtcg
     1861 atgccttcgg tctggcgtgc cgcgtcgagg acggcaagta ccatcgcgtc cgcagccgcc
     1921 gcgccgacca gatctgtcag cttcgcatct gccgtgtcct ccaggcgcac gcgatttacg
     1981 tacggggaaa gcgcgaagat cgtatcaccg tcccgcatgg tatgcatcgg acggattgcc
     2041 cgcgccagac catcgtgcgc gagatcggcg atcttgatca attggctctt ctcgagatcc
     2101 gcattcgttg ctataagccc gagagtcgta ttggcccgcg atgccacttc gtccgggacc
     2161 tcgtctttca ggtggtagag aagcggtgta tcgaagccgc cgtctgtggc gtagagtttt
     2221 cctgtcaggg ggtggatgag accgccaagc gagttgacaa ctaccagcgc accgacgaca
     2281 atcccgcccg gcaagaccaa tgacgccgtc ccaagtcctc ccttggtctt gatgcatccg
     2341 gggcccttgc ctgcggtcgc gccggctcca acgcccacat tgcctcgtgc gatctcacca
     2401 tccgttgcgg cctccgcagc ttgtcgtccc cagtctgctg tcggaacgat cgtcgggtca
     2461 cttagccgga ggtcgaagat cactgcgccg gacatgacgg ggagaacggc cccatccatc
     2521 cgcaacccca cgccctgctc aaaaagccat tcggccattc ctgccgttgc cgcgaggccg
     2581 aagaggctcc cgcctgtcag tccgatccca tggaccagcg agccgatagc ggtcgcagcg
     2641 agcgcatcgg tattcacggt gcccggatta gacccgcgca ccgacacgcc agcggccgct
     2701 ccctcctgac agaggacggc ggtaaccccg cggcgaacct catcatgcgt gtaatgcccc
     2761 acggtgattc ccggcacatc ggtcaccgcg ttgagcggtc cggagtggag ctggagtcca
     2821 gtatccgatg caggatccat cacttcttcc cctctattca tggccgcatg gagcggatag
     2881 cagtacgttc atattcggtg actctcggtg tgctgatcca cttgtgtgaa tctcgttcac
     2941 ggtagtgcat cccagtttgc ccagaagcag ttctctacac tcgctaacga ggtccgggcg
     3001 ccttttccgt cgcacctcct gcctggtggt tgatattcct tcgcggacca cccggatgac
     3061 ggggcgttgc cccttgacgc gtggaatcgg tgaccgagtg ctggacgagg gggacgttga
     3121 tccacagaac
//
